[Histonet] RE: Cresyl violet stain on 50 um mouse brain sections

[Entwistle, Laura]

We have done the same, but also adding a 50 and 70 ETOH as well.  Our slides are also gel coated.  We also have them in a 1 to 1 alcohol/chloroform, but only have them in for 30 minutes.

Laura

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael, Susan
Sent: Friday, July 29, 2011 6:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections

I am having trouble keeping my sections on the slides when I stain with cresyl violet.  These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water.  Into the cresyl violet solution, then 100% to 95% ETOH.  Is it the water?  Is it the slides?  Any suggestions?

Susan
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