[Histonet] Cresyl violet stain on 50 um mouse brain sections

[Michael, Susan]

I am having trouble keeping my sections on the slides when I stain with cresyl violet.  These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water.  Into the cresyl violet solution, then 100% to 95% ETOH.  Is it the water?  Is it the slides?  Any suggestions?

Susan