[Histonet] Frozen eye problems

[Boyce, Bobbie]

Hello Histoneters,

I'm having problems getting a good section on paraformaldyehyde fixed
adult mouse eyes frozen in TBS tissue freezing medium. One of the
problems was the orentation of them. They were too close to the edge. I
also, wanted to try a harder freezing medium, so I corrected oriented
and used an OCT equivalent tissue freezing medium.  They looked better,
but still not as nice as I think they should be.

My eye contact tells me that they should be cut at -20 for adult and -14
for neonatal. They have me set the knife angle at 0 to -2.5. 

I also tried cutting these as I would cut any other frozen issue, but
I'm just pulling my hair out.

Does anyone have any other suggestions that I might try? Your help would
be greatly appreciated.

Bobbie Boyce
Histology Specialist III
duPont Hospital for Children
Wilmington, DE
302-651-6771 (Lab)
302-651-5010 (Fax)