[Histonet] Re: Recycled alcohol

[Tim Higgins]

Hey Michelle,

The easiest way to find out if it's the recycled regeants is to use fresh
reagents and see how the stain looks.  Obvisously if it still shows sign of
a lack of color or detail than I would think it could be the stains
themselves.

What manufacture and type of Hematoxylin and Eosin are you using?

Thanks,

Tim

Message: 9
Date: Fri, 22 Jul 2011 07:06:19 -0400
From: "histotech <@t> imagesbyhopper.com" <histotech <@t> imagesbyhopper.com>
Subject: [Histonet] Recycled alcohol
To: "Histonet <@t> Lists. Utsouthwestern. Edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A7B6C430-34ED-4666-86E5-8D2FA304FF50 <@t> imagesbyhopper.com>
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Hi Histonetters!

For those who use recycled alcohol, I have a few questions.

Do you use it in your automatic H&E stainers?
Are you having any troubles with proper, consistent H&E staining?

We are using recycled alcohol, and wish to continue to do so IF we can.  We
are having issues with our staining - hematoxylin isn't as purple as it
should be, eosin is way too pink/red. We use recycled alcohol when
deparaffinizing and running down to water. For the record, we are also using
recycled xylene.  After staining, we use 2 recycled 100s and two fresh 100s.
I have increased the hem time, decreased the eosin time, adjusted the acid
alcohol and bluing... I'm tearing my hair out trying to troubleshoot this!
Can anyone help me?

Thanks!
Michelle