[Histonet] Processing

[Rene J Buesa]

Set your formalin at 45ºC, dehydration at room temp with vacuum/pressure and the paraffin as usual.
René J.

--- On Wed, 7/6/11, Nicole Schuster <njbates15 <@t> yahoo.com> wrote:


From: Nicole Schuster <njbates15 <@t> yahoo.com>
Subject: [Histonet] Processing
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, July 6, 2011, 3:57 PM


For large tissue specimens (prostate, placenta, etc.) what temperature do you use for the solutions on your processor? We currently have them all set at 40 degrees Celsius. For paraffin it is set at 60 degrees Celsius. I read the dehydrating and clearing solutions should be at ambient temperatures... Thought I'd check into it. Thanks! :)

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