From lpwenk <@t> sbcglobal.net Fri Jul 1 04:47:11 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Jul 1 04:47:15 2011 Subject: [Histonet] School Program Director Position Message-ID: <270673B48E754C689A9E18C640862B98@HP2010> I?m planning on retiring September 2012 (next year). Our hospital (William Beaumont Hospital, Royal Oak, Michigan) is currently looking for someone to co-teach with me for the next year. We train 2 HT and 4 HTL each year. The program director does all the lectures and labs for histotechnology, immunology (IHC and IF), electron microscope, muscle enzyme histochemistry, molecular pathology, shares responsibility with the MT program director for education methodology and management classes, and does research/process improvement projects with the students, which they then write up as PowerPoints and present to the residents, pathologists and techs. We?re looking for someone with: - HTL(ASCP) - Baccalaureate degree (BA or BS) minimum - 5 years histotechnology experience minimum - 3 years teaching experience minimum (including preparing presentations, and writing goals,objectives and exams) For more information, go to the Careers Page on the NSH website www.nsh.org - On middle, right side, under Quick Link, click on Careers Center (I don?t know if you have to ?register? yourself at this time) - Under Job Seeker, click on View Jobs - Click on Program Director, Schools of Histotechnology, posted on 06/30/11 Peggy A. Wenk, HTL(ASCP)SLS Program Director Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 From settembr <@t> umdnj.edu Fri Jul 1 06:51:44 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Fri Jul 1 06:51:53 2011 Subject: [Histonet] Luciferase In-Reply-To: <4E0C8D23.2B7F.00C9.1@geisinger.edu> References: <1309449651.4032.YahooMailClassic@web65709.mail.ac4.yahoo.com> <4E0C684E.2B7F.00C9.1@geisinger.edu> <4E0CA760.6010008@umdnj.edu> <4E0C8D23.2B7F.00C9.1@geisinger.edu> Message-ID: This is so much fun. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, June 30, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu; McAuliffe, William Subject: Re: [Histonet] Luciferase You can, but the retrieval involves a young priest and an old priest. ff McAuliffe 6/30/2011 12:42 PM >>> It is a devil to stain. Geoff On 6/30/2011 12:13 PM, Angela Bitting wrote: > ok there is a really BAD religious joke coming soon, isn't there? > > >>>> Rene J Buesa 6/30/2011 12:00 PM>>> > You would have to have an antibody to it and I don't know any one > exists, but I can be wrong. > Ren? J. > > --- On Thu, 6/30/11, sgoebel@mirnarx.com wrote: > > > From: sgoebel@mirnarx.com > Subject: [Histonet] Luciferase > To: histonet@lists.utsouthwestern.edu > Date: Thursday, June 30, 2011, 11:55 AM > > > Can you stain for luciferase in tissue? > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Fri Jul 1 11:46:01 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Jul 1 11:46:06 2011 Subject: [Histonet] MOHS Technician Needed in Denver Message-ID: MPath Search Partners, a division of Allied Search Partners is currently looking for a MOHS Histology Technician in Denver, CO. Schedule: M-F Full Time Day Shift Requirements: Previous work experience in MOHS surgery Summary of Job: Preparation of tissue specimens Cutting frozen sections on the cryostat Maintenance of laboratory equipment, reagents, and slide organization Opportunity for patient care as a surgical assistant including triage of patients, assistance with operative procedures, suture removal and maintenance of surgical equipment Salary: Dependant on experience Full Medical and other benefits offered To apply: Please send resume to alyssa@alliedsearchpartners.com -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From d-emge <@t> northwestern.edu Fri Jul 1 11:47:11 2011 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Fri Jul 1 11:47:28 2011 Subject: [Histonet] Shrunken Fibers Problem Mouse Muscle Fresh Frozen Sections H&E Stained Message-ID: <000901cc380e$86304630$9290d290$@edu> I am having a big problem with the H&E stained 6 micron frozen sections of mouse muscle tissue. The Immunofluorescence of adjacent sections on another slide are fine, but on the H&E stained slides the fibers have shrunk and there are large spaces between the fibers. The sections are from the same tissue, adjacent slides, cut at the same time. The sections are from fresh muscle tissue sticking out of tragacanth paste that was flash frozen in a beaker of isopentane in liquid nitrogen. The sections on slides were brought to the lab on dry ice and stored in our -20 for H&E staining the next day. The sections looked fine under the scope unstained, but shrunk and had large spaces after H&E staining. I did not see freezing artifact: holes, spidery blown out cells etc on the unstained slides just before staining and as I said the IF adjacent slides of the same tissue looks fine. I believe the tissue is reacting with the alcohol and xylene since for IF only water based reagents are used. Stain protocol: Air dry and bring sections to RT, 10% NBF 5min, 95% Etoh 1min, 80% Etoh 1 min, H2O 2min, Harris Hematoxylin 2 mins, H20 1min, 0.5% HCl 70% Etoh 2 secs, H20 2mins, Sat. Lithium Carbonate blueing 30secs, H20 1min, 80% Etoh, Eosin, - dehydrate, clear, mount w/ xlyene based mount. All other frozen section tissue types look great stained with this protocol. The H&E actually looked great, just not the muscle section morphology after H&E staining. I see Google images of frozen muscle sections stained with H&E so there must be a way to do the stain without this effect on frozen muscle sections. I would be grateful to have anyone that does work with frozen mouse muscle tissue give me some advice and detailed protocols. Thank you, Donna Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 d-emge@northwestern.edu 312-503-2679 From sgoebel <@t> mirnarx.com Fri Jul 1 12:48:33 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Jul 1 12:48:36 2011 Subject: [Histonet] Vasculature in tumors Message-ID: Hello all and happy long weekend (yes our boss gave us the rest of the day off at 2!!!) Looking to stain vasculature in tumors. I did trichrome, pentachrome, and PAS (with digestion) and am being told that these are not optimal for what they are looking for. Any other ideas to stain blood vessels would be awesome!! Thanks ya'll! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From hborgeri <@t> wfubmc.edu Fri Jul 1 12:53:40 2011 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Jul 1 12:53:58 2011 Subject: [Histonet] RE: Vasculature in tumors In-Reply-To: References: Message-ID: <1199C3D2DD5872438C656168966E32650484EF@EXCHDB1.medctr.ad.wfubmc.edu> Hi Sarah, You can try CD34, VEGF, and FVIII. Hermina Hermina M. Borgerink, MALS, HT, HTL(ASCP)QIHC Wake Forest University Primate Center Department of Pathology Medical Center Blvd. Winston-Salem. N C? 27157 Tel. (336) 716-1538 Fax (336) 716-1515 Email: hborgeri@wakehealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Friday, July 01, 2011 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vasculature in tumors Hello all and happy long weekend (yes our boss gave us the rest of the day off at 2!!!) Looking to stain vasculature in tumors. I did trichrome, pentachrome, and PAS (with digestion) and am being told that these are not optimal for what they are looking for. Any other ideas to stain blood vessels would be awesome!! Thanks ya'll! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Fri Jul 1 12:54:43 2011 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Jul 1 12:54:46 2011 Subject: [Histonet] Vasculature in tumors In-Reply-To: References: Message-ID: You are probably looking for capillaries maybe arterioles so there wont be any elastic lamina for the pentachrome stain to detect. Instead try IHC with an endothelial marker like VE Cadherin (best) or in a pinch, CD31 or PECAM. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Fri, 1 Jul 2011 12:48:33 -0500 > From: sgoebel@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Vasculature in tumors > > Hello all and happy long weekend (yes our boss gave us the rest of the > day off at 2!!!) > > > > Looking to stain vasculature in tumors. I did trichrome, pentachrome, > and PAS (with digestion) and am being told that these are not optimal > for what they are looking for. Any other ideas to stain blood vessels > would be awesome!! > > > > Thanks ya'll! > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Fri Jul 1 13:02:30 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Jul 1 13:02:34 2011 Subject: [Histonet] ...maybe a little more specific Message-ID: So am getting lots of responses for IHC stains (which is ok), but I am doing these stains on mouse xenografts and the background is getting in my way a lot. Was just trying to find a simple special that would do the trick =) If IHC is the only way, so be it...was just hoping... What about Verhoeff??? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From Ronald.Houston <@t> nationwidechildrens.org Fri Jul 1 13:10:24 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jul 1 13:10:31 2011 Subject: [Histonet] RE: ...maybe a little more specific In-Reply-To: References: Message-ID: Sarah, if you don't want to go to the hassle of working up antibodies on xenografts, you could use Ulex Europaeus lectin histochemistry which should work on any species - great for capillaries Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Friday, July 01, 2011 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ...maybe a little more specific So am getting lots of responses for IHC stains (which is ok), but I am doing these stains on mouse xenografts and the background is getting in my way a lot. Was just trying to find a simple special that would do the trick =) If IHC is the only way, so be it...was just hoping... What about Verhoeff??? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rjbuesa <@t> yahoo.com Fri Jul 1 14:58:06 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 1 14:58:09 2011 Subject: [Histonet] RE: ...maybe a little more specific In-Reply-To: Message-ID: <1309550286.412.YahooMailClassic@web65714.mail.ac4.yahoo.com> Sarah: Ronald's?suggestion of using Ulex europaeus lectin is a great idea. I second it. Ren? J. --- On Fri, 7/1/11, Houston, Ronald wrote: From: Houston, Ronald Subject: [Histonet] RE: ...maybe a little more specific To: "'sgoebel@mirnarx.com'" , "histonet@lists.utsouthwestern.edu" Date: Friday, July 1, 2011, 2:10 PM Sarah, if you don't want to go to the hassle of working up antibodies on xenografts, you could use Ulex Europaeus lectin histochemistry which should work on any species - great for capillaries Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Friday, July 01, 2011 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ...maybe a little more specific So am getting lots of responses for IHC stains (which is ok), but I am doing these stains on mouse xenografts and the background is getting in my way a lot.? Was just trying to find a simple special that would do the trick =) If IHC is the only way, so be it...was just hoping... What about Verhoeff??? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BBranton <@t> sarapath.com Fri Jul 1 16:08:14 2011 From: BBranton <@t> sarapath.com (Brian Branton) Date: Fri Jul 1 16:08:46 2011 Subject: [Histonet] Remove my name Message-ID: Hello Histonet, Please remove my name from the list, as I am on vacation next week. I will re-subscribe when I return. Thanks, Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology Sarasota Professional Enterprises II (941) 362-8963 (941) 362-8964 FAX From Diane.Tokugawa <@t> kp.org Fri Jul 1 18:14:41 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Fri Jul 1 18:14:58 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 07/01/2011 and will not return until 07/11/2011. Note: For Cytology issues, please call Wanda at 8-421-5426, For Histology issues, please call the general histology lab 8-421- 5408, Mario 8-421-4961 (day), Kiran 8-421-5404 (late afternoon/eve) or Wanda Lau for Cyto/Histo issues 8-421-5426. From pruegg <@t> ihctech.net Sat Jul 2 11:08:30 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jul 2 11:08:34 2011 Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 In-Reply-To: <7DA79EBDBD92BF408EF392413737878D3942992FAA@NADCWPMSGCMS01.hca.corpad.net> References: <7DA79EBDBD92BF408EF392413737878D3942992FAA@NADCWPMSGCMS01.hca.corpad.net> Message-ID: <2522F1B6DC464C8996D641BAFC6C4BEF@prueggihctechlt> I thought Ventana and Dako both had FDA approved Her2, I know Dako has Hercept Test. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Tuesday, June 28, 2011 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, & HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, & HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ken_Marissael <@t> vwr.com Sat Jul 2 13:03:00 2011 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Sat Jul 2 13:03:06 2011 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 07/02/2011 and will not return until 07/12/2011. I will be away on vacation and will not have access to either computer or Blackberry. While I am away, please contact VWR Healthcare Customer Service at 877-881-1192. If you have an urgent requirement, please contact either Jackie Zerillo (609)410-6152 or Scott Nuetzel (410)302-3045 From coralmani <@t> yahoo.co.in Sat Jul 2 14:38:21 2011 From: coralmani <@t> yahoo.co.in (mani kandan) Date: Sat Jul 2 14:38:30 2011 Subject: [Histonet] antibodies for immunohistochemistry Message-ID: <1309635501.45557.YahooMailClassic@web94715.mail.in2.yahoo.com> Hai,? ? ? i am planing to ihc on mouse femour and ?cells derived from femour BM(cell blocks), my target antigens are c-kit,sca-1,pcna,ki67.so nw i am in dielamma from which company i have to select antibody. i am looking for your valuable sugessions. M.Manikandan,Researcher,Stemcell unit,King Saud university,Riyadh,KSA+966552012697 From gu.lang <@t> gmx.at Sun Jul 3 04:28:51 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Jul 3 04:28:55 2011 Subject: AW: [Histonet] VENTANA ULTRA & ER,PR,HER2 In-Reply-To: <2522F1B6DC464C8996D641BAFC6C4BEF@prueggihctechlt> References: <7DA79EBDBD92BF408EF392413737878D3942992FAA@NADCWPMSGCMS01.hca.corpad.net> <2522F1B6DC464C8996D641BAFC6C4BEF@prueggihctechlt> Message-ID: <3670E386E3A84DCE93B572E264AA1223@dielangs.at> The Her2 CB11 clone of Ventana is FDA approved. In the package insert of the Her2 4B5 clone is stated, that the antibody was compared to CB11 and shows same or better performance. The CB11 clone was exchanged through 4B5. On the NordiQC website Ventana 4B5 and Dako Herceptest are called FDA approved. http://www.nordiqc.org/Run-30-B10/Assessment/assessment-B10-HER2.htm So in my opinion the company wouldn't exchange its antibody, if it wouldn't be approved now or soon. Gudrun Lang -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Samstag, 02. Juli 2011 18:09 An: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] VENTANA ULTRA & ER,PR,HER2 I thought Ventana and Dako both had FDA approved Her2, I know Dako has Hercept Test. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Tuesday, June 28, 2011 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, & HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, & HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Sun Jul 3 11:50:59 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Sun Jul 3 11:51:04 2011 Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 In-Reply-To: <3670E386E3A84DCE93B572E264AA1223@dielangs.at> References: <7DA79EBDBD92BF408EF392413737878D3942992FAA@NADCWPMSGCMS01.hca.corpad.net> <2522F1B6DC464C8996D641BAFC6C4BEF@prueggihctechlt> <3670E386E3A84DCE93B572E264AA1223@dielangs.at> Message-ID: I think Barbara was referring to the approval for the ER, PR and Her2 antibodies being for use with the Benchmark XT stainers and that they aren't yet approved for use on the ULTRA stainers using newer formulated bulk reagents. It's funny because they both use the same detection kits and antibodies. The formulation for the bulk reagents is different and it is a new instrument, so I think that's why they need another approval from the FDA. The antibodies were already approved for use with the Benchmark XT stainers. We aren't running Ventana's new Dual ISH Her2 probe kit but we will be looking at it. It does seem to have some perks from the lab's point of view. The instrument does the entire ISH procedure/stain. No more having to mix the reagents for the pretreatment as with Abbott's Pathvysion Kit, repeated pipetting, 22 x 22 coverslips or rubber cement. It's even worse if you don't have a VP2000 pretreatment unit, then you're stuck doing FISH pretreatment by hand with coplin jars in waterbaths. You don't need a fluorescent scope to see the slide with Ventana's new kit and it would be easy for the pathologist to make sure the correct area is scored for Her2 amplification (as can be a problem with the pathologist circling the area of interest on an H&E and the tech matching it up to another unstained serial section with Her2 FISH). I know a lot of labs use the Pathvysion kit "off label" and dilute the probe and change other things from the original FDA approved protocol. Then again, even deparaffinzing the slide using xylene is "off label", since it was approved using Hemo-D substitute. I imagine it would be harder to go "off label" with Ventana's software. I guess you've got to weigh the pros and cons as with everything. Mark Tarango On Sun, Jul 3, 2011 at 2:28 AM, Gudrun Lang wrote: > The Her2 CB11 clone of Ventana is FDA approved. In the package insert of > the > Her2 4B5 clone is stated, that the antibody was compared to CB11 and shows > same or better performance. > The CB11 clone was exchanged through 4B5. > > On the NordiQC website Ventana 4B5 and Dako Herceptest are called FDA > approved. > http://www.nordiqc.org/Run-30-B10/Assessment/assessment-B10-HER2.htm > > So in my opinion the company wouldn't exchange its antibody, if it wouldn't > be approved now or soon. > > Gudrun Lang > > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy > Ruegg > Gesendet: Samstag, 02. Juli 2011 18:09 > An: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu > Betreff: RE: [Histonet] VENTANA ULTRA & ER,PR,HER2 > > I thought Ventana and Dako both had FDA approved Her2, I know Dako has > Hercept Test. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Barbara.Crill@LPNT.net > Sent: Tuesday, June 28, 2011 8:54 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 > > We are investigating getting the Ventana Ultra. > I discovered that the ER, PR, & HER2 are not yet FDA approved. > > If you are using the Ventana Ultra how are you doing the ER, PR, & HER2? > Do you use the Benchmark XT? > > Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? > > > > > ANTOINETTE CRILL, > E-mail: barbara.crill@LPNT.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shehnazster <@t> gmail.com Sun Jul 3 12:32:21 2011 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Sun Jul 3 12:32:26 2011 Subject: [Histonet] corrective action for failed water test Message-ID: Hello Histonetters, I was wondering what the corrective action for failed water testing would be? Thanks in advance S Kahn From rjbuesa <@t> yahoo.com Sun Jul 3 12:41:57 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jul 3 12:42:00 2011 Subject: [Histonet] corrective action for failed water test In-Reply-To: Message-ID: <1309714917.37008.YahooMailClassic@web65709.mail.ac4.yahoo.com> You cannot have corrective actions for each different task. You have to have a general procedure for corrective actions for any task. Usually the procedure is determined by your Human Resources department and you have to include it in your Standard Operation Procedure manual and each member of?the staff?has to sign acknowledging the procedure. Then and only then you can follow the different steps in the corrective action. Ren? J. --- On Sun, 7/3/11, shehnaz khan wrote: From: shehnaz khan Subject: [Histonet] corrective action for failed water test To: histonet@lists.utsouthwestern.edu Date: Sunday, July 3, 2011, 1:32 PM Hello Histonetters, I was wondering what the? corrective action for failed water testing would be? Thanks in advance S Kahn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sun Jul 3 22:10:04 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Jul 3 22:10:10 2011 Subject: [Histonet] Vasculature, Non IHC Message-ID: Hi Sarah, I think Verhoeff vanGieson would be much more effective here than a pentachrome. It is possible for subsequent steps in the pentachrome procedures to over differentiate the elastic or possibly just obscure it. Furthermore the vanGieson counterstain contrasts the elastic better. If you are trying to get away from the whole VVG staining while demonstrating the vasculature, you could also try a Picrosirius Red. This will deminstrate the collagen fibers nicely. Please let me know if you need a nice procedure for this. It can be a easy stain, but it is just as easy to goof up if the solutions aren't prepared and maintained properly. If I think of another I'll post it, but that is what popped into mind first. There are also a number of other Trichrome techniques you could try. Possibly Gomori's, Mallory's or Lillie's might work better for this than the commonly used Masson's. By the way, I too was taken aback by the total absence of traditional histochemical suggestions. Is IHC such a crutch these days, or am I just getting old? Amos Message: 4 Date: Fri, 1 Jul 2011 13:02:30 -0500 From: Subject: [Histonet] ...maybe a little more specific To: Message-ID: Content-Type: text/plain; charset="us-ascii" So am getting lots of responses for IHC stains (which is ok), but I am doing these stains on mouse xenografts and the background is getting in my way a lot. Was just trying to find a simple special that would do the trick =) If IHC is the only way, so be it...was just hoping... What about Verhoeff??? From amosbrooks <@t> gmail.com Sun Jul 3 22:19:38 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Jul 3 22:19:42 2011 Subject: [Histonet] antibodies for immunohistochemistry Message-ID: Hi, C-Kit and PCNA from Dako work well on mouse tissues. Ki67 rabbit monoclonal clone SP6 from various vendors works well too. I have tried Thermo and Biocare Medical with great success on murine samples. I have no experience with SCA-1, although I can say the historic re-enactment group (SCA) is definately more interesting than the antibody :-) Have a great weekend, Amos Message: 2 Date: Sun, 3 Jul 2011 01:08:21 +0530 (IST) From: mani kandan Subject: [Histonet] antibodies for immunohistochemistry To: histonet@lists.utsouthwestern.edu Message-ID: <1309635501.45557.YahooMailClassic@web94715.mail.in2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hai, i am planing to ihc on mouse femour and cells derived from femour BM(cell blocks), my target antigens are c-kit,sca-1,pcna,ki67.so nw i am in dielamma from which company i have to select antibody. i am looking for your valuable sugessions. From rjbuesa <@t> yahoo.com Mon Jul 4 10:34:24 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 4 10:34:30 2011 Subject: [Histonet] Vasculature, Non IHC In-Reply-To: Message-ID: <1309793664.83385.YahooMailClassic@web65708.mail.ac4.yahoo.com> Amos: I think that the IHC suggestions abounded because, put it simply, they are "so simple" (pardon the pun!). Consider that all the steps are common to any IHC procedure and only the antibody changes. You will have all is necessary to complete them. HC, on the other hand, will require a set of reagents different for each procedure, plus it will also require the ability to complete it, based on experience on each HC. That is why, I think, people recommend IHC more; it is simpler. Ren? J. --- On Sun, 7/3/11, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] Vasculature, Non IHC To: sgoebel@mirnarx.com, histonet@lists.utsouthwestern.edu Date: Sunday, July 3, 2011, 11:10 PM Hi Sarah, ? ???I think Verhoeff vanGieson would be much more effective here than a pentachrome. It is possible for subsequent steps in the pentachrome procedures to over differentiate the elastic or possibly just obscure it. Furthermore the vanGieson counterstain contrasts the elastic better. ? ? If you are trying to get away from the whole VVG staining while demonstrating the vasculature, you could also try a Picrosirius Red. This will deminstrate the collagen fibers nicely. Please let me know if you need a nice procedure for this. It can be a easy stain, but it is just as easy to goof up if the solutions aren't prepared and maintained properly. If I think of another I'll post it, but that is what popped into mind first. ? ? There are also a number of other Trichrome techniques you could try. Possibly Gomori's, Mallory's or Lillie's might work better for this than the commonly used Masson's. ? ? By the way, I too was taken aback by the total absence of traditional histochemical suggestions. Is IHC such a crutch these days, or am I just getting old? Amos Message: 4 Date: Fri, 1 Jul 2011 13:02:30 -0500 From: Subject: [Histonet] ...maybe a little more specific To: Message-ID: ? ? ??? Content-Type: text/plain;? ? ???charset="us-ascii" So am getting lots of responses for IHC stains (which is ok), but I am doing these stains on mouse xenografts and the background is getting in my way a lot.? Was just trying to find a simple special that would do the trick =) If IHC is the only way, so be it...was just hoping... What about Verhoeff??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Mon Jul 4 16:02:44 2011 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Mon Jul 4 16:02:56 2011 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 07/01/2011 and will not return until 07/05/2011. I am out of the office today. I will return on July 5th. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From wilson6848 <@t> yahoo.com Mon Jul 4 18:15:26 2011 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Mon Jul 4 18:15:31 2011 Subject: [Histonet] Looking for Position in IHC/HISTOLOGY Message-ID: <1309821326.26450.YahooMailRC@web120902.mail.ne1.yahoo.com> ?? ?I am an HTL(ASCP)QIHC Certified Histotech with an extensive experience in IHC. I am looking for position in IHC/HISTOLOGY.? Willing to relocate. ?Thanks,?Wilson. From Erin.Martin <@t> ucsf.edu Mon Jul 4 21:56:50 2011 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Jul 4 21:56:55 2011 Subject: [Histonet] Re: Failed water test Message-ID: <379A927A452F3D43A3C8705F4E67905F166C05F43D@EX05.net.ucsf.edu> Hi, In our lab, the water is tested every 6 months. If it fails, we disinfect the DI dispenser (to rule out that the nozzle was contaminated) and resubmit a sample to micro. If it fails second time we would would contact the vendor to figure out what the problem is - we've never had to go that far. Hope this helps! Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 From sdeppeler <@t> ksu.edu.sa Tue Jul 5 03:34:04 2011 From: sdeppeler <@t> ksu.edu.sa (Stacy Deppeler) Date: Tue Jul 5 03:34:11 2011 Subject: [Histonet] Re: antibodies for immunohistochemistry Message-ID: Hi Mani, As a fellow KSU researcher I would suggest trying companies such as Dako, Santa Cruz, RnD Systems, Abcam, and Millipore as they all have distributors in KSA who can get your antibodies in a fairly reasonable timeframe. From your list I have only used the Ki67 from SantaCruz, which is alright but I would expect that Dako may have a much better one. If you need help with distributors names then contact me off list. Good luck! -- Stacy Deppeler. Research Assistant Department of Ophthalmology King Abdulaziz University Hospital Riyadh, KSA Date: Sun, 3 Jul 2011 01:08:21 +0530 (IST) From: mani kandan Subject: [Histonet] antibodies for immunohistochemistry To: histonet@lists.utsouthwestern.edu Message-ID: <1309635501.45557.YahooMailClassic@web94715.mail.in2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hai,? ? ? i am planing to ihc on mouse femour and ?cells derived from femour BM(cell blocks), my target antigens are c-kit,sca-1,pcna,ki67.so nw i am in dielamma from which company i have to select antibody. i am looking for your valuable sugessions. M.Manikandan,Researcher, Stemcell unit,King Saud university,Riyadh,KSA+966552012697 From ree3 <@t> leicester.ac.uk Tue Jul 5 04:22:57 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Tue Jul 5 04:23:06 2011 Subject: [Histonet] RE: ...maybe a little more specific In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A101768B23167A@EXC-MBX3.cfs.le.ac.uk> In our paper on peliosis hepatis in mice we used peroxidase conjugated wheat germ agglutinin purchased from Sigma, worked a treat. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: 01 July 2011 19:03 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ...maybe a little more specific So am getting lots of responses for IHC stains (which is ok), but I am doing these stains on mouse xenografts and the background is getting in my way a lot. Was just trying to find a simple special that would do the trick =) If IHC is the only way, so be it...was just hoping... What about Verhoeff??? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue Jul 5 04:45:44 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Jul 5 04:45:47 2011 Subject: [Histonet] corrective action for failed water test In-Reply-To: References: Message-ID: Are you asking for suggestions of what steps to take when the water testing fails? Are are you asking how correct someone who incorrectly did the testing of the water? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: shehnaz khan Sent: Sunday, July 03, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] corrective action for failed water test Hello Histonetters, I was wondering what the corrective action for failed water testing would be? Thanks in advance S Kahn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Tue Jul 5 06:54:56 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Tue Jul 5 06:55:09 2011 Subject: [Histonet] Failed water test Message-ID: <8CE09217E7DF4A5-2FC-4F574@webmail-d029.sysops.aol.com> Some years ago, our deionized water system became contaminated by a gram negative organism. (I don't know what the bacteria live on, in the dark, with only deionized water to "eat"). We have a contract with a deionized water company, but they said it would be impossible to sterilize the whole system, with many labs on different floors and water lines weaving through the building and dead ends here and there. Now we screw filters on each spigot we use. The resistivity is in the right range, and the culture tests are negative. When the filter gets clogged up, the water runs slowly, and we know its time to change the filter. Michael Titford Pathology USA Mobile AL USA From Sara.Lees <@t> covance.com Tue Jul 5 07:50:31 2011 From: Sara.Lees <@t> covance.com (Lees, Sara) Date: Tue Jul 5 07:50:47 2011 Subject: [Histonet] Pan B cells ICC method Message-ID: <248907F494F5B740A86B9C6FF4C3D59F5F133D@crwxch04.ent.covance.com> Hi, Does anyone have a good Pan B cell method for immunocytochemistry, to be used on formalin fixed mouse/rat tissue in paraffin. As its a method I have never done before. Kind Regards Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From d-emge <@t> northwestern.edu Tue Jul 5 08:50:46 2011 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Tue Jul 5 08:50:54 2011 Subject: [Histonet] Mouse Muscle Fresh Frozen Sections H&E Stained, large spaces between fibers Message-ID: <003201cc3b1a$8ac55b40$a05011c0$@edu> I would be grateful to have some advice and detailed protocols from anyone that works with frozen mouse muscle tissue. I am having a big problem with the H&E stained 6 micron frozen sections of mouse muscle tissue. The fibers have shrunk and there are large spaces between the fibers. The Immunofluorescence on another slide are fine. The sections are from the same tissue, adjacent slides, cut at the same time. The sections are from fresh muscle tissue sticking out of tragacanth paste that was flash frozen in a beaker of isopentane in liquid nitrogen. The sections on slides were brought to the lab on dry ice and stored in our -20 for H&E staining the next day. The sections looked fine under the scope unstained, but shrank and had large spaces after H&E staining. I did not see freezing artifact: holes, spidery blown out cells etc on the unstained slides just before staining and as I said the IF adjacent slides of the same tissue looks fine. I believe the tissue is reacting with the alcohol and xylene since for IF only water based reagents are used. Stain protocol: Air dry and bring sections to RT, 10% NBF 5min, 95% Etoh 1min, 80% Etoh 1 min, H2O 2min, Harris Hematoxylin 2 mins, H20 1min, 0.5% HCl 70% Etoh 2 secs, H20 2mins, Sat. Lithium Carbonate blueing 30secs, H20 1min, 80% Etoh, Eosin, - dehydrate, clear, mount w/ xlyene based mount. All other frozen section tissue types look great stained with this protocol. The H&E actually looked great, just not the muscle section morphology after H&E staining. I see Google images of frozen muscle sections stained with H&E so there must be a way to do the stain without this effect on frozen muscle sections. Thank you, Donna Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 d-emge@northwestern.edu 312-503-2679 From Marcia_Gaiser <@t> ssmhc.com Tue Jul 5 09:14:46 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Tue Jul 5 09:14:55 2011 Subject: [Histonet] FT histotech position in Oklahoma City OK Message-ID: <728F817C02110E498D803A7C3B0C6248068D43B5A6@S009-APEXM06.ds.ad.ssmhc.com> POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) ? or ? other nationally recognized certifying agency acceptable to the Laboratory Director ? or ? experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.com, Ad# 10762, or contact Anna King at (405) 272-6105 for more information. Thank you! Anna King HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From histotech <@t> imagesbyhopper.com Tue Jul 5 09:42:07 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Jul 5 09:42:22 2011 Subject: [Histonet] Isopropyl alcohol Message-ID: We recycle our isopropyl alcohol. We have been told by our microwave manufacturer that we cannot use the recycled alcohol on the microwave processor. As a result, I am trying to find additional uses for the recycled isopropyl. Is there any reason why I can't use the iso in place of ethanol when running the slides down to water on the H&E stainer? Any others uses? Any input is greatly appreciated! :o) Michelle From dreynold <@t> mdanderson.org Tue Jul 5 09:20:51 2011 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Tue Jul 5 09:43:17 2011 Subject: [Histonet] Brigati Stable DAB Message-ID: <785BBF0C5F49CE41BA74460A43A08F022EA0189F1E@DCPWVMBXC0VS3.mdanderson.edu> I just learned the company making the Brigati Stable DAB is no longer making it. Does anyone know if someone else has been able to pick up this formulation and produce it or are planning to make it. We have been using this for over 20 years and I really hate to change. What DAB are others using that you are happy with? Donna Reynolds HT (ASCP), Chief Histology Lab Core IHC Lab Dept. Cancer Biology, SRB 1.660 713-792-8106 From Montina.VanMeter <@t> pbrc.edu Tue Jul 5 09:58:53 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Tue Jul 5 09:59:32 2011 Subject: [Histonet] Brigati Stable DAB In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F022EA0189F1E@DCPWVMBXC0VS3.mdanderson.edu> References: <785BBF0C5F49CE41BA74460A43A08F022EA0189F1E@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: Invitrogen - cat# 750118 Sent from my iPhone On Jul 5, 2011, at 10:46 AM, "Reynolds,Donna M" wrote: > I just learned the company making the Brigati Stable DAB is no longer making it. Does anyone know if someone else has been able to pick up this formulation and produce it or are planning to make it. We have been using this for over 20 years and I really hate to change. What DAB are others using that you are happy with? > > Donna Reynolds HT (ASCP), > Chief Histology Lab > Core IHC Lab > Dept. Cancer Biology, SRB 1.660 > 713-792-8106 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jul 5 10:58:56 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 5 10:59:00 2011 Subject: [Histonet] Isopropyl alcohol In-Reply-To: Message-ID: <1309881536.51662.YahooMailClassic@web65711.mail.ac4.yahoo.com> First of all, if you recycle correctly your 2-propanol there is no objective reason why you cannot use it in your MW processor, unless your provider wants you to buy?your?2-propanol from them. Ren? J. --- On Tue, 7/5/11, histotech@imagesbyhopper.com wrote: From: histotech@imagesbyhopper.com Subject: [Histonet] Isopropyl alcohol To: "Histonet@Lists. Utsouthwestern. Edu" Date: Tuesday, July 5, 2011, 10:42 AM We recycle our isopropyl alcohol. We have been told by our microwave manufacturer that we cannot use the recycled alcohol on the microwave processor. As a result, I am trying to find additional uses for the recycled isopropyl.? Is there any reason why I can't use the iso in place of ethanol when running the slides down to water on the H&E stainer? Any others uses? Any input is greatly appreciated!? :o) Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chak_bou <@t> yahoo.com Tue Jul 5 11:35:35 2011 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Tue Jul 5 11:35:40 2011 Subject: [Histonet] SAG2Y antibody Message-ID: <1309883735.14646.YahooMailClassic@web161803.mail.bf1.yahoo.com> Hi Histonetters, Does anyone using SAG2Y?toxoplasma?antibody? if so, can you share with me the staining protocol? Thank you! Chakib Boussahmain HTL(ASCP) MIT From leiker <@t> buffalo.edu Tue Jul 5 11:39:16 2011 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Tue Jul 5 11:39:26 2011 Subject: [Histonet] Vasculature in tumors In-Reply-To: References: Message-ID: I agree... I've used CD31 to stain tumor vasculature. Some tumor vasculature isn't even lined with endothelium; there's just blood "channels." But most should be lined. Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, July 01, 2011 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors You are probably looking for capillaries maybe arterioles so there wont be any elastic lamina for the pentachrome stain to detect. Instead try IHC with an endothelial marker like VE Cadherin (best) or in a pinch, CD31 or PECAM. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Fri, 1 Jul 2011 12:48:33 -0500 > From: sgoebel@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Vasculature in tumors > > Hello all and happy long weekend (yes our boss gave us the rest of the > day off at 2!!!) > > > > Looking to stain vasculature in tumors. I did trichrome, pentachrome, > and PAS (with digestion) and am being told that these are not optimal > for what they are looking for. Any other ideas to stain blood vessels > would be awesome!! > > > > Thanks ya'll! > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Jul 5 12:10:02 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jul 5 12:10:07 2011 Subject: [Histonet] Vasculature in tumors - long response In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE0646F756@SBS2K8.premierlab.local> Hello all I have been following this discussion and I'm going to put in my 2 cents. Tumor vasculature has traditionally been measured with IHC and CD31 or possibly another IHC marker. We use CD31 here for mouse xenograft samples. We have several antibodies we use and it is dependent upon the species. Human tumors (not xenografts) are stained with CD31 but a different antibody (Dako) than the mouse xenografts (Dianova). For other species, canine, porcine, etc we use factor VIII. Factor VIII is not the best marker since it may not pick up fine capillaries but we have been unsuccessful in getting a CD31 to work in FFPE tissue for those species and we have tried several antibodies. The dako antibody will also work on rabbit samples. I can't remember off the top of my head on what we use for rat but it may be Factor VIII. I believe that a large number of publications in which tumor vasculature is measured CD31 has been used. I do not believe that any special stain will provide you with the information that is needed for this type of staining and analysis, especially if you are going to publish the results. The IHC stain for CD31 (for mouse xenograft samples) had been tricky in the past since most antibodies that stain mouse endothelial cells only worked in frozen sections or zinc fixed (not zinc formalin) paraffin embedded samples. Santa Cruz did have a goat polyclonal antibody that did work on FFPE samples for a period of time, early 2000's, but that antibody no longer works in FFPE samples (only frozen, something about the goat dying and the new lots of antisera did not work in FFPE tissues). If you are staining mouse xenograft samples then the Dianova antibody would be the best one to use in my opinion. I need to state that we have not used or even tried VE Cadherin or other IHC markers for vessels, so I really can't comment on those, they may work very well. We use CD31 since we are also running analysis and in order to do that you need a very clean stain and we can achieve that with the Dianova antibody, prior to the Dianova antibody we had used the santa cruz antibody (we had the particular lot number that worked in FFPE tissues, which also worked in multiple species, I do miss that antibody). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leiker, Merced Sent: Tuesday, July 05, 2011 10:39 AM To: 'JR R'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors I agree... I've used CD31 to stain tumor vasculature. Some tumor vasculature isn't even lined with endothelium; there's just blood "channels." But most should be lined. Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, July 01, 2011 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors You are probably looking for capillaries maybe arterioles so there wont be any elastic lamina for the pentachrome stain to detect. Instead try IHC with an endothelial marker like VE Cadherin (best) or in a pinch, CD31 or PECAM. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Fri, 1 Jul 2011 12:48:33 -0500 > From: sgoebel@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Vasculature in tumors > > Hello all and happy long weekend (yes our boss gave us the rest of the > day off at 2!!!) > > > > Looking to stain vasculature in tumors. I did trichrome, pentachrome, > and PAS (with digestion) and am being told that these are not optimal > for what they are looking for. Any other ideas to stain blood vessels > would be awesome!! > > > > Thanks ya'll! > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Jul 5 14:15:00 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jul 5 14:15:05 2011 Subject: [Histonet] Vasculature in tumors - long response In-Reply-To: <14E2C6176416974295479C64A11CB9AE0646F756@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE0646F756@SBS2K8.premierlab.local> Message-ID: <79275D4AB748418A87E8E2A6E5ABBD38@Patsyoffice> I agree with Liz on most all of this about CD31 and love the Dianova rat anti ms ab on mouse xenographs, I have also tried the BC rat < ms anti CD31 and it works but not as good as Dianova for the really early vessels. Another alternative I have used in the past on rats besides F8 and SMA is SC goat < CD34 but the problem with CD34 is that it stains lots of other things besides endothelial cells, it stains all progenitor cells, but that has been an option for rat tissue. Always looking for good endothelial cell markers for rat especially but all species. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, July 05, 2011 11:10 AM To: 'Leiker, Merced'; 'JR R'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors - long response Hello all I have been following this discussion and I'm going to put in my 2 cents. Tumor vasculature has traditionally been measured with IHC and CD31 or possibly another IHC marker. We use CD31 here for mouse xenograft samples. We have several antibodies we use and it is dependent upon the species. Human tumors (not xenografts) are stained with CD31 but a different antibody (Dako) than the mouse xenografts (Dianova). For other species, canine, porcine, etc we use factor VIII. Factor VIII is not the best marker since it may not pick up fine capillaries but we have been unsuccessful in getting a CD31 to work in FFPE tissue for those species and we have tried several antibodies. The dako antibody will also work on rabbit samples. I can't remember off the top of my head on what we use for rat but it may be Factor VIII. I believe that a large number of publications in which tumor vasculature is measured CD31 has been used. I do not believe that any special stain will provide you with the information that is needed for this type of staining and analysis, especially if you are going to publish the results. The IHC stain for CD31 (for mouse xenograft samples) had been tricky in the past since most antibodies that stain mouse endothelial cells only worked in frozen sections or zinc fixed (not zinc formalin) paraffin embedded samples. Santa Cruz did have a goat polyclonal antibody that did work on FFPE samples for a period of time, early 2000's, but that antibody no longer works in FFPE samples (only frozen, something about the goat dying and the new lots of antisera did not work in FFPE tissues). If you are staining mouse xenograft samples then the Dianova antibody would be the best one to use in my opinion. I need to state that we have not used or even tried VE Cadherin or other IHC markers for vessels, so I really can't comment on those, they may work very well. We use CD31 since we are also running analysis and in order to do that you need a very clean stain and we can achieve that with the Dianova antibody, prior to the Dianova antibody we had used the santa cruz antibody (we had the particular lot number that worked in FFPE tissues, which also worked in multiple species, I do miss that antibody). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leiker, Merced Sent: Tuesday, July 05, 2011 10:39 AM To: 'JR R'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors I agree... I've used CD31 to stain tumor vasculature. Some tumor vasculature isn't even lined with endothelium; there's just blood "channels." But most should be lined. Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, July 01, 2011 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors You are probably looking for capillaries maybe arterioles so there wont be any elastic lamina for the pentachrome stain to detect. Instead try IHC with an endothelial marker like VE Cadherin (best) or in a pinch, CD31 or PECAM. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Fri, 1 Jul 2011 12:48:33 -0500 > From: sgoebel@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Vasculature in tumors > > Hello all and happy long weekend (yes our boss gave us the rest of the > day off at 2!!!) > > > > Looking to stain vasculature in tumors. I did trichrome, pentachrome, > and PAS (with digestion) and am being told that these are not optimal > for what they are looking for. Any other ideas to stain blood vessels > would be awesome!! > > > > Thanks ya'll! > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Jul 5 18:13:37 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jul 5 18:13:54 2011 Subject: [Histonet] RE: Brigati Stable DAB In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F022EA0189F1E@DCPWVMBXC0VS3.mdanderson.edu> References: <785BBF0C5F49CE41BA74460A43A08F022EA0189F1E@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A7157188901F9@xmdb02.nch.kids> I have found the following very easy to use (an in-house method): Principle: The peroxidase enzyme coupled to the ABC Complex is demonstrated using hydrogen peroxide as a substrate and 3,3 diaminobenzidine (DAB) as the electron acceptor. This causes polymerisation of the DAB resulting in a solvent resistant precipitate. All histochemical counterstains (except those containing a yellow or brown dye) can be used. Solutions: 1. 3,3, Diaminobenzidine Tetrahydrochloride Grade II (Sigma D5637). 2. Tris Buffer-Wash 3. 30% Hydrogen Peroxidase 4. DAB Stock Solutions Caution Irritant 1. Weigh out 2g DAB in a fume hood 2. Dissolve in 50ml Tris Buffer-Wash 3. Label fifty 1ml reagent vials 4. Aliquot 0.5ml DAB solution in each tube 5. Freeze and Store at -20oC 5. DAB Working Solution: 1. Defrost one vial of DAB Stock. 2. Add to 50ml Tris Buffer-Wash 3. Add 50?l Hydrogen Peroxide 4. Incubate slides for 5-7min until sections appear brown Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M Sent: Wednesday, 6 July 2011 12:21 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Brigati Stable DAB I just learned the company making the Brigati Stable DAB is no longer making it. Does anyone know if someone else has been able to pick up this formulation and produce it or are planning to make it. We have been using this for over 20 years and I really hate to change. What DAB are others using that you are happy with? Donna Reynolds HT (ASCP), Chief Histology Lab Core IHC Lab Dept. Cancer Biology, SRB 1.660 713-792-8106 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From andreahooper <@t> rocketmail.com Tue Jul 5 18:24:10 2011 From: andreahooper <@t> rocketmail.com (andreahooper@rocketmail.com) Date: Tue Jul 5 18:25:13 2011 Subject: [Histonet] Vasculature in tumors - long response In-Reply-To: <79275D4AB748418A87E8E2A6E5ABBD38@Patsyoffice> References: <14E2C6176416974295479C64A11CB9AE0646F756@SBS2K8.premierlab.local><79275D4AB748418A87E8E2A6E5ABBD38@Patsyoffice> Message-ID: <46560247-1309908305-cardhu_decombobulator_blackberry.rim.net-2090221244-@b25.c23.bise6.blackberry> I also agree with Liz's well said synopsis. Be aware that in mouse and human CD34 is not as "pan" EC as CD31 or VE-cadherin, it is very endothelial bed selective. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Patsy Ruegg" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Tue, 5 Jul 2011 13:15:00 To: 'Elizabeth Chlipala'; 'Leiker, Merced'; 'JR R'; Subject: RE: [Histonet] Vasculature in tumors - long response I agree with Liz on most all of this about CD31 and love the Dianova rat anti ms ab on mouse xenographs, I have also tried the BC rat < ms anti CD31 and it works but not as good as Dianova for the really early vessels. Another alternative I have used in the past on rats besides F8 and SMA is SC goat < CD34 but the problem with CD34 is that it stains lots of other things besides endothelial cells, it stains all progenitor cells, but that has been an option for rat tissue. Always looking for good endothelial cell markers for rat especially but all species. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, July 05, 2011 11:10 AM To: 'Leiker, Merced'; 'JR R'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors - long response Hello all I have been following this discussion and I'm going to put in my 2 cents. Tumor vasculature has traditionally been measured with IHC and CD31 or possibly another IHC marker. We use CD31 here for mouse xenograft samples. We have several antibodies we use and it is dependent upon the species. Human tumors (not xenografts) are stained with CD31 but a different antibody (Dako) than the mouse xenografts (Dianova). For other species, canine, porcine, etc we use factor VIII. Factor VIII is not the best marker since it may not pick up fine capillaries but we have been unsuccessful in getting a CD31 to work in FFPE tissue for those species and we have tried several antibodies. The dako antibody will also work on rabbit samples. I can't remember off the top of my head on what we use for rat but it may be Factor VIII. I believe that a large number of publications in which tumor vasculature is measured CD31 has been used. I do not believe that any special stain will provide you with the information that is needed for this type of staining and analysis, especially if you are going to publish the results. The IHC stain for CD31 (for mouse xenograft samples) had been tricky in the past since most antibodies that stain mouse endothelial cells only worked in frozen sections or zinc fixed (not zinc formalin) paraffin embedded samples. Santa Cruz did have a goat polyclonal antibody that did work on FFPE samples for a period of time, early 2000's, but that antibody no longer works in FFPE samples (only frozen, something about the goat dying and the new lots of antisera did not work in FFPE tissues). If you are staining mouse xenograft samples then the Dianova antibody would be the best one to use in my opinion. I need to state that we have not used or even tried VE Cadherin or other IHC markers for vessels, so I really can't comment on those, they may work very well. We use CD31 since we are also running analysis and in order to do that you need a very clean stain and we can achieve that with the Dianova antibody, prior to the Dianova antibody we had used the santa cruz antibody (we had the particular lot number that worked in FFPE tissues, which also worked in multiple species, I do miss that antibody). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leiker, Merced Sent: Tuesday, July 05, 2011 10:39 AM To: 'JR R'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors I agree... I've used CD31 to stain tumor vasculature. Some tumor vasculature isn't even lined with endothelium; there's just blood "channels." But most should be lined. Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, July 01, 2011 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors You are probably looking for capillaries maybe arterioles so there wont be any elastic lamina for the pentachrome stain to detect. Instead try IHC with an endothelial marker like VE Cadherin (best) or in a pinch, CD31 or PECAM. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Fri, 1 Jul 2011 12:48:33 -0500 > From: sgoebel@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Vasculature in tumors > > Hello all and happy long weekend (yes our boss gave us the rest of the > day off at 2!!!) > > > > Looking to stain vasculature in tumors. I did trichrome, pentachrome, > and PAS (with digestion) and am being told that these are not optimal > for what they are looking for. Any other ideas to stain blood vessels > would be awesome!! > > > > Thanks ya'll! > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue Jul 5 18:37:47 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Jul 5 18:37:50 2011 Subject: [Histonet] corrective action for failed water test In-Reply-To: References: Message-ID: <69128314AA8C4F92BB75768505C2FD28@HP2010> We?ve had several water issues over the years. First question is ? what failed? pH? Bacteria count? Silicates? Conductivity? Take your readings, and compile a chart showing what the levels are SUPPOSED to be, what they HAD been the last couple of times (when they were ?normal? for you), and what they are NOW. Contact your head pathologist and department manager. Show them the chart. Tell them why this is bad. Have them notify the lab techs and other pathologists of the problem and the possible effect. - increased bacteria ? watch for false positives - increased silicates or conductivity ? silver stains more likely to precipitate - pH off ? stains may not work right (not stain at all, stain light, stain dark, background staining, precipitate ? depends what the stain is, and what the pH is) Contact your water treatment department in your facility. Ours is in the Power House. You might have to contact Maintenance, or Safety, to find out who is in charge. Call a meeting. See if they know of any change. (in our situation,one time, they forgot to change the filter in the deionizer for 1.5 years, instead of every 3 months.). (Another time, they decided to try out the backup deionizer unit that had never been used in 20 years. Didn?t tell us, and didn?t look inside to see 20 years worth of rust. Talk about lots of contamination and conductivity levels to the moon!) Call the local water treatment plant. Find out if they had made any changes. (Our situation, one time, the water treatment plant had increased the amount of phosphates, to help counteract the lead leaching out of lead pipes in the poorer part of the counties (trying to make it safer for people who were drinking the water). But increased phosphates led to an increase of bacteria.) Contact the people who would have the blue prints of the d. water system (maintenance?). Was it hooked up correctly. D. water is supposed to be a loop system. If the water isn?t used, it flows back to the deionizer. It?s easier to remove small amount of ions from previous d. water, than to continuously take new tap water and deionizing it. (In our situation, one time, they built a new lab, and dead-ended the d. water pipes. That?s where the bacteria grew, and then flowed backwards through all the pipes.) After we made the pipes into a loop, we ended up installing UV lights near the deionizers, to zap the water before it went into the deionizer, to kill the bacteria. In a couple of weeks, are bacteria numbers were back to almost non-existent. In the meantime, buy some filters that fit on the taps, install, and write the date on top of the filter. When it starts running slow, it?s time to change. Since the date is written on top, you know how often you have to change. And you will know when the water is getting cleaner, and you can go longer periods of time before the next change. We order ours through US Filters, which is now Siemens. I don?t know what the order number is. If you need it, contact me at work at pwenk@beaumont.edu If it?s taking too long to correct, see who has Millipore water filters built into their lines (mol. path? special chemistry?). You can fill up gallon jugs each day from these other departments. Or buy distilled water from the grocery store (check it?s numbers first). Use for special stains, particularly silver stains and microorganism stains. (BTW ? some of these incidents are all related ? maintenance switching from paper reminders to computer, forgot to put ?change filters? in computer, so they didn?t get changed, AND at the same time the water treatment plant in the county decide to increase phosphates.) Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 From: shehnaz khan Sent: Tuesday, July 05, 2011 10:34 AM To: Lee & Peggy Wenk Subject: Re: [Histonet] corrective action for failed water test Hi What steps to take when the water testing fails - please. Thanks in advance. S Kahn On Tue, Jul 5, 2011 at 12:45 PM, Lee & Peggy Wenk wrote: Are you asking for suggestions of what steps to take when the water testing fails? Are are you asking how correct someone who incorrectly did the testing of the water? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: shehnaz khan Sent: Sunday, July 03, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] corrective action for failed water test Hello Histonetters, I was wondering what the corrective action for failed water testing would be? Thanks in advance S Kahn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Wed Jul 6 04:15:39 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Jul 6 04:15:53 2011 Subject: [Histonet] Isopropyl alcohol In-Reply-To: <1309881536.51662.YahooMailClassic@web65711.mail.ac4.yahoo.com> References: <1309881536.51662.YahooMailClassic@web65711.mail.ac4.yahoo.com> Message-ID: <9DFE44C5-210F-4195-973C-274E8DE428CF@imagesbyhopper.com> Rene, I was just as surprised! When we purchased the mw, we were led to believe that we could use the recycled alcohol, but I was told later that we should not for quality reasons - that recycled is never 100% pure and that water contamination will adversely affect the processing. So, can I use it on my H&E stainer without negatively affecting the stain quality? As always, thanks for you thoughts, Michelle On Jul 5, 2011, at 11:58 AM, Rene J Buesa wrote: > First of all, if you recycle correctly your 2-propanol there is no objective reason why you cannot use it in your MW processor, unless your provider wants you to buy your 2-propanol from them. > Ren? J. > > --- On Tue, 7/5/11, histotech@imagesbyhopper.com wrote: > > From: histotech@imagesbyhopper.com > Subject: [Histonet] Isopropyl alcohol > To: "Histonet@Lists. Utsouthwestern. Edu" > Date: Tuesday, July 5, 2011, 10:42 AM > > We recycle our isopropyl alcohol. We have been told by our microwave manufacturer that we cannot use the recycled alcohol on the microwave processor. > > As a result, I am trying to find additional uses for the recycled isopropyl. Is there any reason why I can't use the iso in place of ethanol when running the slides down to water on the H&E stainer? > > Any others uses? > > Any input is greatly appreciated! :o) > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Wed Jul 6 05:34:52 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Jul 6 05:35:16 2011 Subject: [Histonet] School Program Director Position In-Reply-To: <270673B48E754C689A9E18C640862B98@HP2010> References: <270673B48E754C689A9E18C640862B98@HP2010> Message-ID: <1CD7D42C763549719B0C2F40F3661BA3@HP2010> To answer a couple of questions that have been asked of me: A) What constitutes "teaching experience"? Obviously, teaching in a NAACLS accredited HT or HTL program, even if it's only 1 topic. But, it could also include teaching a course at a community college, or some lectures or workshops at state or national meetings, or even doing some in-services. But it should include writing objectives and evaluations (tests questions). There is no requirement as to needing "X number of hours" of teaching. Just that there has been some teaching in the last 3 years. The more, the better. B) Why all the requirements for teaching experience, supervisory experience, etc. listed on the NSH jobs webpage? Is there some "wiggle room"? The requirements listed are the NAACLS requirements for Program Directors. If you are missing a little bit, that's what the 1 year co-teach with me is for, to pick up what you are missing (never did EM, for example, or not a lot of experience writing exam questions). Sorry, must have BA/BS - NAACLS requirement. Since this is a HTL program, along with HT, we need someone to have passed the HTL exam. So no wiggle room in either of these. (Hint for those people who think they want to be a program director some day - look at the requirements on my job listing, realize that the program director positions for all HT and HTL programs have the same requirements. Some places require a master's degree. There will be a lot of us program directors retiring in the next 10+ years, so start working on getting the degree, the HTL certification, the teaching experience, even if it's just presenting at state and/or national symposiums.) www.nsh.org click on career center, under job seeker click on view jobs C) Are you going to stop teaching at NSH? No, I hope not. I plan to continue to give presentations at NSH (if they accept my abstracts), and actually hope to increase the number of states I can give talks at. (hint, hint - invite me). Maybe write an article or two or three. Do some more teleconferences. Help new HT/HTL programs get off the ground, if they need help. Maybe consult. I'd like to think I have more options to do more teaching in more places. My husband has been retired for 4.5 years, we've had a motor home for 0.5 years, and he's wondering when I'm going to catch up with him, so we can travel more. Any other questions, toss them my way. Peggy A. Wenk, BA, BS, HTL(ASCP)SLS Program Director, Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 www.beaumont.edu/alliedhealth -----Original Message----- From: Lee & Peggy Wenk Sent: Friday, July 01, 2011 5:47 AM To: Histonet Subject: [Histonet] School Program Director Position I?m planning on retiring September 2012 (next year). Our hospital (William Beaumont Hospital, Royal Oak, Michigan) is currently looking for someone to co-teach with me for the next year. We train 2 HT and 4 HTL each year. The program director does all the lectures and labs for histotechnology, immunology (IHC and IF), electron microscope, muscle enzyme histochemistry, molecular pathology, shares responsibility with the MT program director for education methodology and management classes, and does research/process improvement projects with the students, which they then write up as PowerPoints and present to the residents, pathologists and techs. We?re looking for someone with: - HTL(ASCP) - Baccalaureate degree (BA or BS) minimum - 5 years histotechnology experience minimum - 3 years teaching experience minimum (including preparing presentations, and writing goals,objectives and exams) For more information, go to the Careers Page on the NSH website www.nsh.org - On middle, right side, under Quick Link, click on Careers Center (I don?t know if you have to ?register? yourself at this time) - Under Job Seeker, click on View Jobs - Click on Program Director, Schools of Histotechnology, posted on 06/30/11 Peggy A. Wenk, HTL(ASCP)SLS Program Director Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brian <@t> prometheushealthcare.com Wed Jul 6 08:01:24 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Wed Jul 6 08:01:36 2011 Subject: [Histonet] Histology Director Opening Message-ID: <016f01cc3bdc$d2dec340$789c49c0$@com> Physician owned laboratory located in Houston TX is currently searching for a Histology Director. Job desc: This candidate will oversee all technical and management duties in the histology laboratory and will be an integral part of the management team. * Responsible for all functions of the lab including compliance with all accrediting agencies. * Personnel management of the laboratory. * Safety and educational programs and compliance. * Oversee purchasing of supplies and equipment. * Budgeting and financial reporting. * Interaction with clients on technical and lab operation issues. * Develop and implement policies and procedures for the department. * Evaluate and recommend new procedures, instrumentation, and technology. * Ongoing training/education with histotechnology staff. Job Requirements: B.S. or B.A. Degree * Registered as Histotechnologist with ASCP or other national certifying agency. * Excellent oral and written communication skills. * Five or more years of experience in supervision and leadership of outpatient laboratory operations. * Information systems and Microsoft applications experience. * Immunohistochemistry experience/accredidation a plus. * Gross examination capabilities a plus. Please contact me today for immediate consideration. Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From jengirl1014 <@t> yahoo.com Wed Jul 6 08:17:29 2011 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Wed Jul 6 08:17:36 2011 Subject: [Histonet] Thermo Stainmate Message-ID: <1309958249.52399.YahooMailRC@web125408.mail.ne1.yahoo.com> Hi all! I hope you can help me out!? My lab recently obtained the Thermo Stainmate slide stainer with 10 baths.? I was wondering if anyone had a H & E protocol for it that worked well.? I have one protocol that I've been using, but the changes require more than 10 baths.? Thanks so much for all of your help!? Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 933 720 Rutland Avenue Baltimore, MD? 21205 phone:???? fax:???????? 410-955-9677 cell:???????? 443-631-6361 e-mail:? jsipes1@jhmi.edu From sbreeden <@t> nmda.nmsu.edu Wed Jul 6 08:28:28 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jul 6 08:28:34 2011 Subject: [Histonet] OT: Wednesday Half-Wit Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF847@nmdamailsvr.nmda.ad.nmsu.edu> I used to use Sub-X in the processor. Changing paraffin was no problem because I'd just drain it into a Styrofoam box, allow it to set, seal it up, mark it "waste paraffin" and put it into the regular trash. Easy, right? Well - then I changed back to using xylene. First time I changed paraffin, I used my tried-and-true method, only to arrive at the lab in the morning to find a partially dissolved Styrofoam box on the counter, surrounded by a solidified pool of paraffin. Eureka! Xylene dissolves Styrofoam! It was not pretty but I think I've invented a new space-age compound. Fortunately, I was struck by an Inspiration - use an empty xylene/alcohol bottle with the top cut off (so I can insert the paraffin drain). I don't know why I'm posting this except to show that when confronted with an unusual circumstance, women rule! Now, back to work. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From candice_camille <@t> yahoo.com Wed Jul 6 08:51:29 2011 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Wed Jul 6 08:51:34 2011 Subject: [Histonet] Ehrlich hematoxylin Message-ID: <1309960289.61746.YahooMailRC@web125417.mail.ne1.yahoo.com> Hello All I was hoping that someone could answer a question for me. I work in a small lab?were we?need to make or own ehrlich hematoxylin. We have all the chemical needed to?make it. My question is... How long does it normally take the sodium iodate to dissolve and at what point?do you add it in. Ours is not dissoving even after we left it over night. Please Help!!!!? Thanks ?I remain yours truely, Candice Camille From sgoebel <@t> mirnarx.com Wed Jul 6 09:01:54 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Jul 6 09:01:58 2011 Subject: [Histonet] OT: Wednesday Half-Wit In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF847@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B051DF847@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: We used to put a red bio-hazard bag (the heavy duty one's) into a cardboard box. This usually did not leak. Just saying if you still want to use your box method =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, July 06, 2011 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Wednesday Half-Wit I used to use Sub-X in the processor. Changing paraffin was no problem because I'd just drain it into a Styrofoam box, allow it to set, seal it up, mark it "waste paraffin" and put it into the regular trash. Easy, right? Well - then I changed back to using xylene. First time I changed paraffin, I used my tried-and-true method, only to arrive at the lab in the morning to find a partially dissolved Styrofoam box on the counter, surrounded by a solidified pool of paraffin. Eureka! Xylene dissolves Styrofoam! It was not pretty but I think I've invented a new space-age compound. Fortunately, I was struck by an Inspiration - use an empty xylene/alcohol bottle with the top cut off (so I can insert the paraffin drain). I don't know why I'm posting this except to show that when confronted with an unusual circumstance, women rule! Now, back to work. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Wed Jul 6 10:02:03 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Jul 6 10:02:45 2011 Subject: [Histonet] CBG Recycler Giveaway References: Message-ID: CBG Biotech Benchtop Solvent Recycler Model MSLV-03U, has only been used occasionally, free to a good laboratory home. If you would like this instrument, only requirement is that you pay shipping, please send email off-list to: gagnone@kgh.kari.net Recycler product information here: Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From pkarlisch <@t> hmc.psu.edu Wed Jul 6 10:31:54 2011 From: pkarlisch <@t> hmc.psu.edu (karlisch, patricia) Date: Wed Jul 6 10:32:00 2011 Subject: [Histonet] Slide Labelers Message-ID: Is anyone else out in histoland having issues with the Leica Slide labeler not printing 'permanently' on their recommended slides. We are using the recommended clipped corner, extra adhesive slides for our H&E but every so often the print rubs off after a couple of days. The slides then need to be paper labeled. I am afraid that over time the slides will not be readable. Pat Patricia Karlisch Supervisor, Histology Pathology and Laboratory Medicine Penn State Hershey Medical Center 500 University Drive, PA 17033 Tel: 717-531-6072 FAX 717-531-7741 pkarlisch@hmc.psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately From MLashus <@t> pathgroup.com Wed Jul 6 10:42:38 2011 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Wed Jul 6 10:44:33 2011 Subject: [Histonet] 1DH1 Antibody Message-ID: <197CD0B02A81F94994A285C59C8AE05C07936955CB@pgnexchange.pathgroup.com> Does anyone have a working protocol for the 1DH1 Antibody to be used on the Ventana XT or Ultra? Thanks, Mighnon Lashus, HT (ASCP) PathGroup Chattanooga 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 Phone: 423-493-0207 Fax: 423-493-0208 Email: mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From flnails <@t> texaschildrens.org Wed Jul 6 10:45:36 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Jul 6 10:45:42 2011 Subject: [Histonet] RE: Slide Labelers In-Reply-To: References: Message-ID: I use a clipped corner slide from Statlab and we do not experience any problems. (IJL-6110) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karlisch, patricia Sent: Wednesday, July 06, 2011 10:32 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Slide Labelers Is anyone else out in histoland having issues with the Leica Slide labeler not printing 'permanently' on their recommended slides. We are using the recommended clipped corner, extra adhesive slides for our H&E but every so often the print rubs off after a couple of days. The slides then need to be paper labeled. I am afraid that over time the slides will not be readable. Pat Patricia Karlisch Supervisor, Histology Pathology and Laboratory Medicine Penn State Hershey Medical Center 500 University Drive, PA 17033 Tel: 717-531-6072 FAX 717-531-7741 pkarlisch@hmc.psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From jcox90 <@t> yahoo.com Wed Jul 6 10:46:05 2011 From: jcox90 <@t> yahoo.com (jcox90@yahoo.com) Date: Wed Jul 6 10:46:09 2011 Subject: [Histonet] Looking for Histology work for month of July Message-ID: <1309967165.50681.YahooMailRC@web161608.mail.bf1.yahoo.com> Hi all, I am starting permanent job Aug 1, looking for fill-in for month of July. I am located in Orange County California. I could travel as far as San Diego. I am HT ascp and have 17 years experience. Anyone needing a vacation?? Have a wonderful day Netters! Jill Cox, HT ASCP From STACEY.LANGENBERG <@t> UCDENVER.EDU Wed Jul 6 10:48:30 2011 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Wed Jul 6 10:48:49 2011 Subject: [Histonet] Slide Labelers Message-ID: <617E6E0E-885F-42BA-BB1D-6823EF0F0283@ucdenver.edu> Is your flash tube flashing? Is it old? Spent? We have 6 of them and have never in the 2 years we have had them seen that problem. Stacey Sent from myTouch 4G ----- Reply message ----- From: "karlisch, patricia" To: "'histonet@lists.utsouthwestern.edu'" Subject: [Histonet] Slide Labelers Date: Wed, Jul 6, 2011 9:34 am Is anyone else out in histoland having issues with the Leica Slide labeler not printing 'permanently' on their recommended slides. We are using the recommended clipped corner, extra adhesive slides for our H&E but every so often the print rubs off after a couple of days. The slides then need to be paper labeled. I am afraid that over time the slides will not be readable. Pat Patricia Karlisch Supervisor, Histology Pathology and Laboratory Medicine Penn State Hershey Medical Center 500 University Drive, PA 17033 Tel: 717-531-6072 FAX 717-531-7741 pkarlisch@hmc.psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jul 6 11:18:31 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Jul 6 11:18:39 2011 Subject: [Histonet] RE: Slide Labelers In-Reply-To: References: Message-ID: Only if the slide does not make it down to the "flash" stage........ Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karlisch, patricia Sent: Wednesday, July 06, 2011 11:32 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Slide Labelers Is anyone else out in histoland having issues with the Leica Slide labeler not printing 'permanently' on their recommended slides. We are using the recommended clipped corner, extra adhesive slides for our H&E but every so often the print rubs off after a couple of days. The slides then need to be paper labeled. I am afraid that over time the slides will not be readable. Pat Patricia Karlisch Supervisor, Histology Pathology and Laboratory Medicine Penn State Hershey Medical Center 500 University Drive, PA 17033 Tel: 717-531-6072 FAX 717-531-7741 pkarlisch@hmc.psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ashley.Troutman <@t> Vanderbilt.Edu Wed Jul 6 11:24:33 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Wed Jul 6 11:24:40 2011 Subject: [Histonet] Vasculature in tumors Message-ID: <7B310892042DA74CB3590053F424CFE6143EE30149@ITS-HCWNEM06.ds.Vanderbilt.edu> We use CD34 for endothelial staining in blood vessels and Podoplanin (D2-40) for lymphatic vessels. These are for humans only. (Mine was worth less than a half-cent...but hopefully helpful.) :) Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Date: Tue, 5 Jul 2011 11:10:02 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] Vasculature in tumors - long response To: "'Leiker, Merced'" , 'JR R' , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE0646F756@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Hello all I have been following this discussion and I'm going to put in my 2 cents. Tumor vasculature has traditionally been measured with IHC and CD31 or possibly another IHC marker. We use CD31 here for mouse xenograft samples. We have several antibodies we use and it is dependent upon the species. Human tumors (not xenografts) are stained with CD31 but a different antibody (Dako) than the mouse xenografts (Dianova). For other species, canine, porcine, etc we use factor VIII. Factor VIII is not the best marker since it may not pick up fine capillaries but we have been unsuccessful in getting a CD31 to work in FFPE tissue for those species and we have tried several antibodies. The dako antibody will also work on rabbit samples. I can't remember off the top of my head on what we use for rat but it may be Factor VIII. I believe that a large number of publications in which tumor vasculature is measured CD31 has been used. I do not believe that any special stain will provide you with the information that is needed for this type of staining and analysis, especially if you are going to publish the results. The IHC stain for CD31 (for mouse xenograft samples) had been tricky in the past since most antibodies that stain mouse endothelial cells only worked in frozen sections or zinc fixed (not zinc formalin) paraffin embedded samples. Santa Cruz did have a goat polyclonal antibody that did work on FFPE samples for a period of time, early 2000's, but that antibody no longer works in FFPE samples (only frozen, something about the goat dying and the new lots of antisera did not work in FFPE tissues). If you are staining mouse xenograft samples then the Dianova antibody would be the best one to use in my opinion. I need to state that we have not used or even tried VE Cadherin or other IHC markers for vessels, so I really can't comment on those, they may work very well. We use CD31 since we are also running analysis and in order to do that you need a very clean stain and we can achieve that with the Dianova antibody, prior to the Dianova antibody we had used the santa cruz antibody (we had the particular lot number that worked in FFPE tissues, which also worked in multiple species, I do miss that antibody). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leiker, Merced Sent: Tuesday, July 05, 2011 10:39 AM To: 'JR R'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors I agree... I've used CD31 to stain tumor vasculature. Some tumor vasculature isn't even lined with endothelium; there's just blood "channels." But most should be lined. Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, July 01, 2011 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Vasculature in tumors You are probably looking for capillaries maybe arterioles so there wont be any elastic lamina for the pentachrome stain to detect. Instead try IHC with an endothelial marker like VE Cadherin (best) or in a pinch, CD31 or PECAM. Jerry Ricks Research Scientist University of Washington Department of Pathology From ross <@t> premierlab.com Wed Jul 6 12:28:33 2011 From: ross <@t> premierlab.com (Ross Benik) Date: Wed Jul 6 12:31:13 2011 Subject: [Histonet] Collagen Type VII Message-ID: <14E2C6176416974295479C64A11CB9AE098C643A@SBS2K8.premierlab.local> Hello Histonet, I'm checking to see if anyone has used (with success) a Collagen Type VII antibody on human tissue for IHC-FFPE sections? We've checked with several vendors and so far all we can find are Col VII antibodies that work only in frozen sections. Thanks! Ross From Timothy.Morken <@t> ucsfmedctr.org Wed Jul 6 13:29:59 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Jul 6 13:30:07 2011 Subject: [Histonet] EM Supervisor job opening soon Message-ID: We will have an EM lab supervisor's job opening soon at UC San Francisco Medical Center. You can search for it at http://www.ucsfhr.ucsf.edu/careers/ The lab has 3 techs now and does EM of kidney disease, kidney, heart, lung, pancreas transplants, as well as IF for kidney, skin and lung, and muscle enzyme histochemistry. Department website: http://pathology.ucsf.edu/clinical/renal/renal-index.html Contact me with questions. Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org From mtitford <@t> aol.com Wed Jul 6 13:37:24 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jul 6 13:37:42 2011 Subject: [Histonet] Ehrlich's Hematoxylin Message-ID: <8CE0A22E2097ED5-1B44-80872@webmail-d139.sysops.aol.com> Candice Camille asks about Ehrlich's hematoxylin- The sodium iodate to ripen the hematoxylin should be added last of all, after all the other ingredients, to ripen the stain. (So you will not see if it has dissolved, or not!). Better to ripen the Ehrlich's hematoxylin naturally. Do not add sodium iodate. Instead place the bottle in a sunny windowsill and shake it up every day. Loosen the stopper and let the hematoxylin breath. It will take a few weeks to ripen (very un-20th Century!). You can use it when it changes color to dark red to maroon color. but when ripened it will last for ever such a long time provided it is not contaminated. It gives good regressive staining and will also stain mucins. Michael Titford Pathology USA Mobile AL USA From ervelazquez <@t> gmail.com Wed Jul 6 14:31:02 2011 From: ervelazquez <@t> gmail.com (Eric Velazquez) Date: Wed Jul 6 14:31:07 2011 Subject: [Histonet] Re: HT Position - Irvine, CA In-Reply-To: References: Message-ID: This position has been filled. Thank you for your interest. -Eric Velazquez Agendia Inc. On Fri, Jun 24, 2011 at 4:56 PM, Eric Velazquez wrote: > Hi Histonet, > > We are currently looking for a full-time Histotechnician (preferably HT > certified) with experience in IHC. Please read the description below and if > you're interested please submit your resume via e-mail to: * > eric.velazquez@agendia.com*. > > *ESSENTIAL DUTIES AND RESPONSIBILITIES* > > - Performs histology aspects of the lab, which includes cutting tissue(frozen, FFPE), > manually staining sections for H&E imaging, IHC staining, loading > slides on ScanScope for imaging and scoring by pathologist > - Maintains laboratory logs so that current information is complete and > readily available to staff, including clinical log sheet, ScanScope > identification > - Performs all aspects of lab support to ensure timely completion of > samples. Reports any discrepancies to the Director Of Laboratory > Operations. > - Performs various maintenance and/or housekeeping tasks to keep > laboratory clean, and ensure ease of use. > - Cleans and fills water baths, bulk reagents, dump waste containers > - Reports any malfunctions of equipment to the Director Of Laboratory > Operations. > - Washes all laboratory dishes to ensure a clean supply when needed. > - Performs data entry tasks in the laboratory and office when needed. > - Follows all Agendia, Inc.?s health and safety policies and procedures > - Performs other related duties as required or assigned > > Thank you for your interest. > > -Eric Velazquez > Agendia Inc. > > > From njbates15 <@t> yahoo.com Wed Jul 6 14:57:50 2011 From: njbates15 <@t> yahoo.com (Nicole Schuster) Date: Wed Jul 6 14:58:03 2011 Subject: [Histonet] Processing Message-ID: For large tissue specimens (prostate, placenta, etc.) what temperature do you use for the solutions on your processor? We currently have them all set at 40 degrees Celsius. For paraffin it is set at 60 degrees Celsius. I read the dehydrating and clearing solutions should be at ambient temperatures... Thought I'd check into it. Thanks! :) Sent from my iPhone From rjbuesa <@t> yahoo.com Wed Jul 6 15:12:38 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 6 15:12:45 2011 Subject: [Histonet] Processing In-Reply-To: Message-ID: <1309983158.77990.YahooMailClassic@web65705.mail.ac4.yahoo.com> Set your formalin at 45?C, dehydration at room temp with vacuum/pressure and the paraffin as usual. Ren? J. --- On Wed, 7/6/11, Nicole Schuster wrote: From: Nicole Schuster Subject: [Histonet] Processing To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 6, 2011, 3:57 PM For large tissue specimens (prostate, placenta, etc.) what temperature do you use for the solutions on your processor? We currently have them all set at 40 degrees Celsius. For paraffin it is set at 60 degrees Celsius. I read the dehydrating and clearing solutions should be at ambient temperatures... Thought I'd check into it. Thanks! :) Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Wed Jul 6 15:27:35 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Wed Jul 6 15:27:44 2011 Subject: [Histonet] question on EM Message-ID: <7111DB39D045004C9CF29E79C71B28BC102C085FD1@CMHEXCC01MBX.childrensmemorial.org> Hi, For those out there that do EM- can you please tell me if you ever just embed and hold a specimen and if so how do you bill it? We just stopped doing them in house and are sending them out and occasionally have a case that is embed only. We are billed with an 88399, but I'm not sure if we can billthe same to the patients? Thanks, Lisa From shehnazster <@t> gmail.com Wed Jul 6 16:19:23 2011 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Wed Jul 6 16:19:28 2011 Subject: [Histonet] safe handling of tissue for histology containing radionuclides Message-ID: Hi Everyone, Could someone kindly shed some light on safe handling of tissue for histology containing radionuclides - to comply with CAP standards. How is this done? What should I include in the policy / work instruction? Thanks in advance S Kahn From laurie.colbert <@t> huntingtonhospital.com Wed Jul 6 16:31:38 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jul 6 16:31:41 2011 Subject: [Histonet] Processing In-Reply-To: References: Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AE2E@EXCHANGE3.huntingtonhospital.com> We heat from 38-40 degrees for all reagents except the paraffin, which is heated to 60 degrees. I don't use any heat at all on my small biopsies. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Schuster Sent: Wednesday, July 06, 2011 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing For large tissue specimens (prostate, placenta, etc.) what temperature do you use for the solutions on your processor? We currently have them all set at 40 degrees Celsius. For paraffin it is set at 60 degrees Celsius. I read the dehydrating and clearing solutions should be at ambient temperatures... Thought I'd check into it. Thanks! :) Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Candy.A.Bales <@t> uth.tmc.edu Wed Jul 6 16:47:27 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Wed Jul 6 16:47:30 2011 Subject: [Histonet] MICROWAVE PROCESSING Message-ID: <62C915811DD5A142851D95CA6BC5D1E41B39DB598D@UTHCMS1.uthouston.edu> On behalf of a colleague, For those who use microwaves for processing, what type of paraffin do you use/prefer? Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Health Science Center Houston School of Dentistry Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From tkngflght <@t> yahoo.com Wed Jul 6 17:11:38 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jul 6 17:11:44 2011 Subject: [Histonet] temps and job seekers... Message-ID: <1309990298.70788.YahooMailClassic@web39422.mail.mud.yahoo.com> Hi All- ? We still need a few really good techs for temp positions.?Day and night shift. Also have a number of permanent (regular) job openings to consider. ? Happy to give you the fifty-cent tour of traveling and help with a resume--call if you are curious! ? Cheryl Kerry, HT(ASCP) ? Full Staff Inc. 800.756.3306 phone & fax ? From Rcartun <@t> harthosp.org Wed Jul 6 19:25:41 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 6 19:25:52 2011 Subject: [Histonet] Dpc4 IHC Message-ID: <4E14C4C6.7400.0077.1@harthosp.org> Is anyone doing IHC for the Dpc4 gene product for adenocarcinoma in biopsy specimens of the pancreas and bile duct and, if so, do you find it useful? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From amitapandey <@t> torrentpharma.com Wed Jul 6 23:22:38 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Wed Jul 6 23:23:19 2011 Subject: [Histonet] Routine H & E stain, In-Reply-To: <1309960289.61746.YahooMailRC@web125417.mail.ne1.yahoo.com> References: <1309960289.61746.YahooMailRC@web125417.mail.ne1.yahoo.com> Message-ID: Hello Histonetters, I am from toxicopathology lab where we perform H&E on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result is good (Purplish pink look stained slide) on all tissue, but our observation is after 5-6 months time this get faded and become towards pinkish type though we can observe the slide. We want to retain its fresh stained color. Please suggest me how to keep stable stain for longer period or do you suggest to switch to any other type of haematoxylin - prepared or commercially available? Looking forward for your feed back. Amita From itai.moshe <@t> mail.huji.ac.il Thu Jul 7 03:22:16 2011 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Thu Jul 7 03:22:21 2011 Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation Message-ID: Dear All, Does leaving the samples (diaphragm, liver) over night in ethanol 70% at 4C during the fixation process, will be better for the tissue fixation, and does not harm the sample ? Does the fixation process should be done straight forward from the first step to the last one without any over night stops (except from the PFA, Bouin's step) ? My fixation protocol is like this: 1) immediately after killing the mouse i'm putting the sections in a fixation solution that is made from: 10ml formaldehyde 37%+5ml PBSx20+85ml DDW - pH 7, Or bouin's solution over night at 4C. 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT 3) Xylen x2 - each for 1Hr at RT. 4) Paraffin x3 - each at 60C for 1Hr. Thank you all very much in advance Itai M Doe From itai.moshe <@t> mail.huji.ac.il Thu Jul 7 04:09:59 2011 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Thu Jul 7 04:10:03 2011 Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101768B2316B3@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A101768B2316B3@EXC-MBX3.cfs.le.ac.uk> Message-ID: I had a problem with liver fixation with PFA, most of the time I've got no signal, so I've switched to Bouin's, and got good resalts, although it is harder to cut bouin's sections with microtom. Could it be because that I've switched from Paraformaldehyde powder to formaldehyde solution (37%) (that contains methanol as a preservative) ? I have one good PFA powder experiment that I've done, and some excellent old experiments that someone else in my lab did many years ago, but i don't know how, all worked great with PFA fixation, but i could not replicate the that. I've tried to do a Formaldehyde 24Hr fixation R.T for the mouse liver samples, but the results was catastrophic and even the DAPI didn't worked. So i thought that maybe that because that the working samples were in 70% ETOH over night. 2011/7/7 Edwards, Richard E. > Leaving tissue overnight in 70% ethanol at 4C should have no detrimental > effect n the tissues, why 4C overnight with Bouin's?? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Itai Moshe > Sent: 07 July 2011 09:22 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation > > Dear All, > Does leaving the samples (diaphragm, liver) over night in ethanol 70% at 4C > during the fixation process, will be better for the tissue fixation, and > does not harm the sample ? > Does the fixation process should be done straight forward from the first > step to the last one without any over night stops (except from the PFA, > Bouin's step) ? > > > My fixation protocol is like this: > 1) immediately after killing the mouse i'm putting the sections in a > fixation solution that is made from: 10ml formaldehyde 37%+5ml PBSx20+85ml > DDW - pH 7, Or bouin's solution over night at 4C. > 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT > 3) Xylen x2 - each for 1Hr at RT. > 4) Paraffin x3 - each at 60C for 1Hr. > > Thank you all very much in advance > > Itai M > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Itai Moshe Mark Pines lab Institute of Animal Sciences, Volcani Center. Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew University of Jerusalem Israel From itai.moshe <@t> mail.huji.ac.il Thu Jul 7 04:19:11 2011 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Thu Jul 7 04:19:16 2011 Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101768B2316BF@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A101768B2316B3@EXC-MBX3.cfs.le.ac.uk> <7722595275A4DD4FA225B92CDBF174A101768B2316BF@EXC-MBX3.cfs.le.ac.uk> Message-ID: Thank's, Did you used it for mouse liver section ? Can you please post a your fixation protocol ? 2011/7/7 Edwards, Richard E. > I have no experience of using Bouin?s fixed tissue for immunos, tho? > it is an excellent fixative for many tinctorial procedures providing the > fixation time is carefully controlled?I do not think that the methanol in > the formalin could affect anything adversely, also 10% neutral buffered > formalin is the fixative of choice for most labs, I have never had > a problem with it.**** > > ** ** > > *From:* Itai Moshe [mailto:itai.moshe@mail.huji.ac.il] > *Sent:* 07 July 2011 10:10 > *To:* Edwards, Richard E.; histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Leaving sample over night in ETOH 70% during > fixation**** > > ** ** > > I had a problem with liver fixation with PFA, most of the time I've got no > signal, so I've switched to Bouin's, and got good resalts, although it is > harder to cut bouin's sections with microtom.**** > > ** ** > > Could it be because that I've switched from Paraformaldehyde powder to > formaldehyde solution (37%) (that contains methanol as a preservative) ?** > ** > > I have one good PFA powder experiment that I've done, and > some excellent old experiments that someone else in my lab did many years > ago, but i don't know how, all worked great with PFA fixation, but i could > not replicate the that.**** > > ** ** > > I've tried to do a Formaldehyde 24Hr fixation R.T for the mouse liver > samples, but the results was catastrophic and even the DAPI didn't worked. > **** > > ** ** > > So i thought that maybe that because that the working samples were in 70% > ETOH over night.**** > > ** ** > > ** ** > > 2011/7/7 Edwards, Richard E. **** > > Leaving tissue overnight in 70% ethanol at 4C should have no detrimental > effect n the tissues, why 4C overnight with Bouin's??**** > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Itai Moshe > Sent: 07 July 2011 09:22 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation > > Dear All, > Does leaving the samples (diaphragm, liver) over night in ethanol 70% at 4C > during the fixation process, will be better for the tissue fixation, and > does not harm the sample ? > Does the fixation process should be done straight forward from the first > step to the last one without any over night stops (except from the PFA, > Bouin's step) ? > > > My fixation protocol is like this: > 1) immediately after killing the mouse i'm putting the sections in a > fixation solution that is made from: 10ml formaldehyde 37%+5ml PBSx20+85ml > DDW - pH 7, Or bouin's solution over night at 4C. > 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT > 3) Xylen x2 - each for 1Hr at RT. > 4) Paraffin x3 - each at 60C for 1Hr. > > Thank you all very much in advance > > Itai M**** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet**** > > > > -- > Itai Moshe > Mark Pines lab > Institute of Animal Sciences, Volcani Center. > Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew > University of Jerusalem > Israel**** > -- Itai Moshe Mark Pines lab Institute of Animal Sciences, Volcani Center. Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew University of Jerusalem Israel From mab70 <@t> medschl.cam.ac.uk Thu Jul 7 04:34:19 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Thu Jul 7 04:35:34 2011 Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation References: Message-ID: Storing your samples overnight in 70% ethanol after fixation won't harm your samples, but they should be adequately fixed prior to this. I do not like xylene for mouse tissues, it makes them too brittle. Instead I used histoclear 11 from National Diagnostics. Histoclear 11 is less expensive than the original histoclear, but performs well in my experience. I found that for most of the tissues I processed (I never tried diaphragm) a shorter schedule worked well and in fact the process you describe may be too long for some tissues. The Society of histotechnologists produces a booklet with suggested processing schedules for a wide range of animals and tissues, I found this invaluable in designing my protocols. My process for mouse tissues took aroung 6 hours using a processing machine equipped with vacuum on all stations. Unfortunately as I have retired I don't have access to my SOP's any more. However, if you obtain the booklet from the Society, you will be able to devise a good protocol. At the end of the day, if your tissues section and stain well, then your process is satisfactory. It's always helpful to compare your sections with other people's if you can then you have a benchmark of quality. This can be difficult and frustrating in a small research lab. You may find that postfixing your liver samples in formol alcohol (90ml absolute ethanol to 10ml 37% formaldehyde or use Pen fix from thermo shandon) will improve the morphology. I used to do this for a couple of hours; you may need to experiment, especially if you are doing immunohistochemistry which is presumably why you are using paraformaldehyde. Good luck Margaret ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Itai Moshe Sent: Thu 07/07/2011 09:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation Dear All, Does leaving the samples (diaphragm, liver) over night in ethanol 70% at 4C during the fixation process, will be better for the tissue fixation, and does not harm the sample ? Does the fixation process should be done straight forward from the first step to the last one without any over night stops (except from the PFA, Bouin's step) ? My fixation protocol is like this: 1) immediately after killing the mouse i'm putting the sections in a fixation solution that is made from: 10ml formaldehyde 37%+5ml PBSx20+85ml DDW - pH 7, Or bouin's solution over night at 4C. 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT 3) Xylen x2 - each for 1Hr at RT. 4) Paraffin x3 - each at 60C for 1Hr. Thank you all very much in advance Itai M Doe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LisaKennedy <@t> catholichealth.net Thu Jul 7 04:52:40 2011 From: LisaKennedy <@t> catholichealth.net (Kennedy, Lisa) Date: Thu Jul 7 04:53:03 2011 Subject: [Histonet] Bone Marrows Message-ID: <117A261944D6524F8BEBA799181EC4B7097D75@chimsx08.CHI.catholichealth.net> Are there any Histo Techs out there who assist with bone marrows IN the operating room? Lisa From CThornton <@t> dahlchase.com Thu Jul 7 05:00:47 2011 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Thu Jul 7 05:02:07 2011 Subject: [Histonet] RE: Bone Marrows In-Reply-To: <117A261944D6524F8BEBA799181EC4B7097D75@chimsx08.CHI.catholichealth.net> References: <117A261944D6524F8BEBA799181EC4B7097D75@chimsx08.CHI.catholichealth.net> Message-ID: I do, as well as several of my coworkers. We assist with bone marrows in inpatient rooms, pediatric sedation, outpatient surgery center, and occasionally in the main OR. What is your question? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kennedy, Lisa [LisaKennedy@catholichealth.net] Sent: Thursday, July 07, 2011 5:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Marrows Are there any Histo Techs out there who assist with bone marrows IN the operating room? Lisa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nelsonrnch <@t> verizon.net Thu Jul 7 05:36:25 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Thu Jul 7 05:36:29 2011 Subject: [Histonet] Routine H & E stain, In-Reply-To: References: <1309960289.61746.YahooMailRC@web125417.mail.ne1.yahoo.com> Message-ID: <1310034985.3692.YahooMailRC@web84308.mail.re1.yahoo.com> Are?you using synthetic xylene in your H&E/COVER SLIPPING protocol.?If yes, you might want to consider ending your protocol with the real thing (XYLENE).?I had the same?situation until we switched to real XYLENE.?No more H&E fading. THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ________________________________ From: "amitapandey@torrentpharma.com" To: Histonet ; histonet-bounces@lists.utsouthwestern.edu; histonet Sent: Wed, July 6, 2011 9:22:38 PM Subject: [Histonet] Routine H & E stain, Hello Histonetters, I am from toxicopathology lab where we perform H&E on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result is good (Purplish pink look stained slide) on all tissue, but? our observation is after 5-6 months time this get faded and become towards pinkish type though we can observe the slide. We want to retain its fresh stained color. Please suggest me how to keep stable stain for longer period or do you suggest to switch to any other type of haematoxylin - prepared or commercially available? Looking forward for your feed back. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nelsonrnch <@t> verizon.net Thu Jul 7 05:43:39 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Thu Jul 7 05:43:42 2011 Subject: [Histonet] Routine H & E stain, In-Reply-To: References: <1309960289.61746.YahooMailRC@web125417.mail.ne1.yahoo.com> Message-ID: <1310035419.32177.YahooMailRC@web84304.mail.re1.yahoo.com> I should also have added not all synthetic Xylenes do this and I also use a Microwave Processor. These variables?may have been a factor. Wonderful world of Histology, TRIAL AND ERROR.? ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ________________________________ From: "amitapandey@torrentpharma.com" To: Histonet ; histonet-bounces@lists.utsouthwestern.edu; histonet Sent: Wed, July 6, 2011 9:22:38 PM Subject: [Histonet] Routine H & E stain, Hello Histonetters, I am from toxicopathology lab where we perform H&E on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result is good (Purplish pink look stained slide) on all tissue, but? our observation is after 5-6 months time this get faded and become towards pinkish type though we can observe the slide. We want to retain its fresh stained color. Please suggest me how to keep stable stain for longer period or do you suggest to switch to any other type of haematoxylin - prepared or commercially available? Looking forward for your feed back. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Thu Jul 7 06:49:01 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Jul 7 06:49:10 2011 Subject: [Histonet] Bone Marrows In-Reply-To: <117A261944D6524F8BEBA799181EC4B7097D75@chimsx08.CHI.catholichealth.net> Message-ID: Yes we do. We assist with all bone marrows regardles of where they are (not hematology). We dress out in scrubs, etc. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From kgrobert <@t> rci.rutgers.edu Thu Jul 7 07:52:24 2011 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Thu Jul 7 07:52:28 2011 Subject: [Histonet] Routine H & E stain, In-Reply-To: References: <1309960289.61746.YahooMailRC@web125417.mail.ne1.yahoo.com> Message-ID: Amita, We are a neurotoxicology lab that also provides histology services for other researchers inside & outside Rutgers, and we use Gill's #3 hematoxylin for our routine H&Es on mouse and rat tissues. No fading that I have noticed in all the years that I have been here. We get ours from Thermo Fisher, or whatever they call themselves nowadays. :o) Good luck, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 > Hello Histonetters, > > I am from toxicopathology lab where we perform H&E on rat tissues and > store these slides for 10 long years. > > We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ > Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. > The staining result is good (Purplish pink look stained slide) on all > tissue, but our observation is after 5-6 months time this get faded and > become towards pinkish type though we can observe the slide. > We want to retain its fresh stained color. > > Please suggest me how to keep stable stain for longer period or do you > suggest to switch to any other type of haematoxylin - prepared or > commercially available? > > Looking forward for your feed back. > Amita > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Thu Jul 7 09:44:35 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 7 09:44:40 2011 Subject: [Histonet] Routine H & E stain, In-Reply-To: Message-ID: <1310049875.90302.YahooMailClassic@web65715.mail.ac4.yahoo.com> It seems that your mounting medium is acid. Try to correct that rather than changing the staining. Ren? J. --- On Thu, 7/7/11, amitapandey@torrentpharma.com wrote: From: amitapandey@torrentpharma.com Subject: [Histonet] Routine H & E stain, To: "Histonet" , histonet-bounces@lists.utsouthwestern.edu, "histonet" Date: Thursday, July 7, 2011, 12:22 AM Hello Histonetters, I am from toxicopathology lab where we perform H&E on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result is good (Purplish pink look stained slide) on all tissue, but? our observation is after 5-6 months time this get faded and become towards pinkish type though we can observe the slide. We want to retain its fresh stained color. Please suggest me how to keep stable stain for longer period or do you suggest to switch to any other type of haematoxylin - prepared or commercially available? Looking forward for your feed back. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jul 7 09:52:18 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 7 09:52:26 2011 Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation In-Reply-To: Message-ID: <1310050338.14182.YahooMailClassic@web65712.mail.ac4.yahoo.com> If you have an overnight step it is better to prolong the formalin fixation instead of leaving the tissues in 70EthOL. Since formalin will require at least 24 hours to completely?bind and 48 to crosslink, if you leave your tissues overnight in 70EthOL the unfixed tissue will be fixed by the alcohol instead and that can cause difficulties in the subsequent tests. Ren? J. --- On Thu, 7/7/11, Itai Moshe wrote: From: Itai Moshe Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation To: histonet@lists.utsouthwestern.edu Date: Thursday, July 7, 2011, 4:22 AM Dear All, Does leaving the samples (diaphragm, liver) over night in ethanol 70% at 4C during the fixation process, will be better for the tissue fixation, and does not harm the sample ? Does the fixation process should be done straight forward from the first step to the last one without any over night stops (except from the PFA, Bouin's step) ? My fixation protocol is like this: 1) immediately after killing the mouse i'm putting the sections in a fixation solution that is made from: 10ml formaldehyde 37%+5ml PBSx20+85ml DDW? - pH 7, Or bouin's solution over night at 4C. 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT 3) Xylen x2 - each for 1Hr at RT. 4) Paraffin x3 - each at 60C for 1Hr. Thank you all very much in advance Itai M Doe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jul 7 09:56:45 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 7 09:56:48 2011 Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation In-Reply-To: Message-ID: <1310050605.87990.YahooMailClassic@web65704.mail.ac4.yahoo.com> Bouin's and PFA are completely different in their action an results. If you get good results with Bouin's use it and try to overcome your cutting difficulties. There should be no differences using PFA or 37% formaldehyde. Methanol is only used to prevent formaldehyde polymerization and does not interfere with fixation. The problem is that you will need longer fixation times to obtain good results when using formaldehyde.Ren? J. --- On Thu, 7/7/11, Itai Moshe wrote: From: Itai Moshe Subject: Re: [Histonet] Leaving sample over night in ETOH 70% during fixation To: "Edwards, Richard E." , histonet@lists.utsouthwestern.edu Date: Thursday, July 7, 2011, 5:09 AM I had a problem with liver fixation with PFA, most of the time I've got no signal, so I've switched to Bouin's, and got good resalts, although it is harder to cut bouin's sections with microtom. Could it be because that I've switched from Paraformaldehyde powder to formaldehyde solution (37%) (that contains methanol as a preservative) ? I have one good PFA? powder experiment that I've done, and some excellent old experiments that someone else in my lab did many years ago, but i don't know how, all worked great with PFA fixation, but i could not replicate the that. I've tried to do a Formaldehyde 24Hr fixation R.T for the mouse liver samples, but the results was catastrophic and even the DAPI didn't worked. So i thought that maybe that because that the working samples were in 70% ETOH over night. 2011/7/7 Edwards, Richard E. > Leaving? tissue overnight in 70% ethanol at 4C should have no detrimental > effect n the tissues, why 4C overnight with Bouin's?? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Itai Moshe > Sent: 07 July 2011 09:22 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leaving sample over night in ETOH 70% during fixation > > Dear All, > Does leaving the samples (diaphragm, liver) over night in ethanol 70% at 4C > during the fixation process, will be better for the tissue fixation, and > does not harm the sample ? > Does the fixation process should be done straight forward from the first > step to the last one without any over night stops (except from the PFA, > Bouin's step) ? > > > My fixation protocol is like this: > 1) immediately after killing the mouse i'm putting the sections in a > fixation solution that is made from: 10ml formaldehyde 37%+5ml PBSx20+85ml > DDW? - pH 7, Or bouin's solution over night at 4C. > 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT > 3) Xylen x2 - each for 1Hr at RT. > 4) Paraffin x3 - each at 60C for 1Hr. > > Thank you all very much in advance > > Itai M > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Itai Moshe Mark Pines lab Institute of Animal Sciences, Volcani Center. Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew University of Jerusalem Israel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Jul 7 10:07:49 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Jul 7 10:08:04 2011 Subject: [Histonet] EM supervisor postion at UC San Francisco, job description Message-ID: Here is the EM job I recently mentioned would be open. See the HR website at http://jobs.ucsfmedicalcenter.org/ Job Description Job ID: 2257 Job Title: Electron Microscopy Lab Supervisor (HISTOTECHNOLOGIST, SUPVR) Job Code: 9068 Department: Pathology-Surgical / Histology Location: Parnassus Full/Part Time: Full-Time Regular/Temporary: Regular Shift: Not Applicable Weekly Hours: 40 Salary Range: $68993.6 - $113776 Union Information: This classification is not represented by a union ________________________________ Return to Previous Page ________________________________ Appointment Type: Career Percentage: 100% Shift: Days Shift Length: 8 hours Work Days: Monday-Friday Department: Pathology Summary of Duties: Under general direction from the Director of Clinical Operations and Medical Director, the incumbent serves as supervisor to 3 Histotechnologists. In addition to supervisory duties, the incumbent serves as a technical expert providing direction to staff as well as performing all technical aspects of diagnostic electron microscopy, immunofluorescence microscopy and muscle histochemistry procedures. As supervisor, incumbent recruits, hires, trains, completes performance evaluations, and resolves employee issues. Provides orientation, completes competency assessments, maintains staff schedules, training and compliance documentation. Implements new procedures as needed, updates and maintains the lab manual and annual reviews required for accreditation. Ensures that all equipment is well-maintained and that staff training and documentation are completed as required. Ensures that QC documentation is complete and quality standards are maintained in the laboratory. Advises and assists researchers planning research projects and determines the ability of the laboratory to accommodate research projects, updating the Medical Director as required. Other duties as assigned. Required Qualifications: * College degree in a biological science, chemistry or a related field or equivalent education and work experience in electron microscopy required, plus three years of senior-level experience in a hospital pathology laboratory performing all aspects of diagnostic electron microscopy work-up. * Extensive knowledge of tissue ultrastructure, including Ultrastructural pathology required. * Knowledge / demonstrated skill in of electron microscope maintenance required. * Excellent interpersonal communication skills required. * Demonstrated ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines required. * Previous, recent supervisory experience in an Electron Microscopy Laboratory required. * Understanding of regulatory requirements required. Preferred Qualifications: * EMSA Certification in Electron Microscopy strongly preferred. * Experience in muscle histochemistry highly desirable. * Formal training in electron microscopy. * Experience with digital EM imaging. * Experience in Immunofluorescence technique. * Laboratory supervisory experience in teaching hospital. Required Licenses/Certifications: * N/A Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org From Barry.R.Rittman <@t> uth.tmc.edu Thu Jul 7 10:48:50 2011 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Jul 7 10:48:54 2011 Subject: [Histonet] Routine H & E stain, In-Reply-To: <1310049875.90302.YahooMailClassic@web65715.mail.ac4.yahoo.com> References: <1310049875.90302.YahooMailClassic@web65715.mail.ac4.yahoo.com> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9018D998FD0@UTHCMS1.uthouston.edu> I believe that this could be a multifactorial problem. There is a tendency for us, "in the interests of efficiency" to process tissue and slides as rapidly as possible. In a lot of cases I suspect that the absolute minimum time in reagents causes some carry over that can result in fading of the stains. The quality of the xylene, as one person already pointed out may be a factor. Even using "real xylene", the composition, sulfur content etc can vary considerably and affect staining. There was some concern in the 1950s over fading of slides containing silver stains due to using xylene. However I still have some stained with silver that were cleared with xylene and still look really good. The use of xylene substitutes has not (to my admittedly imperfect knowledge) been tested over a few decades. It would also be interesting to know whether the type of hematoxylin stain used has any effect on fading of hematoxylin. I have always been an advocate for Ehrlich's hematoxylin and have 50+ year old slides that are still as bright as the day they were stained but not much experience with other hematoxylins. Mounting media may also have an effect. In the days of the dinosaur we used Canada balsam in xylene and later, DPX. Slides mounted in these still look really good, in fact dare I say beautiful. Might be an idea to use some pH paper to check pH of the xylene and mountants. Some have also suggested that exposure to UV light may also be a factor. Not sure that there is much evidence for this. I have seen some slides from the mid 1800s that while not pristine still have some hematoxylin staining. Bottom line, is that we have no bloody idea what is causing your hematoxylin to fade- suggest that failing all else, you pray and enjoy a glass of Zinfandel (Paso Robles 2006 was a really good year). Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, July 07, 2011 9:45 AM To: Histonet; histonet-bounces@lists.utsouthwestern.edu; histonet; amitapandey@torrentpharma.com Subject: Re: [Histonet] Routine H & E stain, It seems that your mounting medium is acid. Try to correct that rather than changing the staining. Ren? J. --- On Thu, 7/7/11, amitapandey@torrentpharma.com wrote: From: amitapandey@torrentpharma.com Subject: [Histonet] Routine H & E stain, To: "Histonet" , histonet-bounces@lists.utsouthwestern.edu, "histonet" Date: Thursday, July 7, 2011, 12:22 AM Hello Histonetters, I am from toxicopathology lab where we perform H&E on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result is good (Purplish pink look stained slide) on all tissue, but? our observation is after 5-6 months time this get faded and become towards pinkish type though we can observe the slide. We want to retain its fresh stained color. Please suggest me how to keep stable stain for longer period or do you suggest to switch to any other type of haematoxylin - prepared or commercially available? Looking forward for your feed back. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ARodriguez <@t> emc.org Thu Jul 7 11:37:43 2011 From: ARodriguez <@t> emc.org (Rodriguez, Arnold) Date: Thu Jul 7 11:38:19 2011 Subject: [Histonet] Tissue Cassette Printer Recommendations Message-ID: <3340FC2AE9CFEE4E9D001D077700C6A21FBD7F81@NT106.info.sys> Hello All, We are interested in purchasing a new cassette printer and I would sincerely appreciate any recommendations for this instrument. We are particularly looking for reliability, print speed, LIS connectivity and barcode technology. Thank you very much. Arnold Rodriguez, HT (ASCP) Supervisor, Anatomic Pathology Eisenhower Medical Center From mfisher <@t> ecrmc.org Thu Jul 7 11:43:40 2011 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Thu Jul 7 11:43:49 2011 Subject: [Histonet] Bone Marrows Message-ID: We assist with bone marrows either at bedside or in Outpatient Observation, never in the OR. All other bone marrows are performed at the Oncologist's office. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From JEllin <@t> yumaregional.org Thu Jul 7 11:48:37 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Jul 7 11:48:42 2011 Subject: [Histonet] RE: Tissue Cassette Printer Recommendations In-Reply-To: <3340FC2AE9CFEE4E9D001D077700C6A21FBD7F81@NT106.info.sys> References: <3340FC2AE9CFEE4E9D001D077700C6A21FBD7F81@NT106.info.sys> Message-ID: I would look at Thermo, Leica and General Data printers . Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Arnold Sent: Thursday, July 07, 2011 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Cassette Printer Recommendations Hello All, We are interested in purchasing a new cassette printer and I would sincerely appreciate any recommendations for this instrument. We are particularly looking for reliability, print speed, LIS connectivity and barcode technology. Thank you very much. Arnold Rodriguez, HT (ASCP) Supervisor, Anatomic Pathology Eisenhower Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited, if you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From liz <@t> premierlab.com Thu Jul 7 11:52:44 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jul 7 11:52:49 2011 Subject: [Histonet] RE: Tissue Cassette Printer Recommendations In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE0989F972@SBS2K8.premierlab.local> I second Jesus's comments especially if you are moving towards bar coding. You will need a cassette printer that can print a high quality 2D bar code. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, July 07, 2011 10:49 AM To: 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Cassette Printer Recommendations I would look at Thermo, Leica and General Data printers . Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Arnold Sent: Thursday, July 07, 2011 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Cassette Printer Recommendations Hello All, We are interested in purchasing a new cassette printer and I would sincerely appreciate any recommendations for this instrument. We are particularly looking for reliability, print speed, LIS connectivity and barcode technology. Thank you very much. Arnold Rodriguez, HT (ASCP) Supervisor, Anatomic Pathology Eisenhower Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited, if you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbrya <@t> lexclin.com Thu Jul 7 11:55:07 2011 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Jul 7 11:55:11 2011 Subject: [Histonet] RE: Tissue Cassette Printer Recommendations In-Reply-To: <14E2C6176416974295479C64A11CB9AE0989F972@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE0989F972@SBS2K8.premierlab.local> Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36E5A@EXCHANGESB> I am intersted in this info also. I would like to know what printers everyone are using with the Vantage system or a bar coding system. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, July 07, 2011 12:53 PM To: 'Jesus Ellin'; 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Cassette Printer Recommendations I second Jesus's comments especially if you are moving towards bar coding. You will need a cassette printer that can print a high quality 2D bar code. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, July 07, 2011 10:49 AM To: 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Cassette Printer Recommendations I would look at Thermo, Leica and General Data printers . Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Arnold Sent: Thursday, July 07, 2011 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Cassette Printer Recommendations Hello All, We are interested in purchasing a new cassette printer and I would sincerely appreciate any recommendations for this instrument. We are particularly looking for reliability, print speed, LIS connectivity and barcode technology. Thank you very much. Arnold Rodriguez, HT (ASCP) Supervisor, Anatomic Pathology Eisenhower Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited, if you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From JEllin <@t> yumaregional.org Thu Jul 7 12:05:11 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Jul 7 12:05:19 2011 Subject: [Histonet] RE: Tissue Cassette Printer Recommendations In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36E5A@EXCHANGESB> References: <14E2C6176416974295479C64A11CB9AE0989F972@SBS2K8.premierlab.local> <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36E5A@EXCHANGESB> Message-ID: I do not have Vantage, so I cannot comment on that. But you have to take into account the environment and the scanning quality of the print. I would also look at space, location, downtime, workflow, even clarity of barcode meaning Datamatirx, ect. There are also issues with specific cassette colors and cassette surface texture playing a part in the scanning. -----Original Message----- From: Carol Bryant [mailto:cbrya@lexclin.com] Sent: Thursday, July 07, 2011 9:55 AM To: 'Elizabeth Chlipala'; Jesus Ellin; 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu Subject: RE: Tissue Cassette Printer Recommendations I am intersted in this info also. I would like to know what printers everyone are using with the Vantage system or a bar coding system. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, July 07, 2011 12:53 PM To: 'Jesus Ellin'; 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Cassette Printer Recommendations I second Jesus's comments especially if you are moving towards bar coding. You will need a cassette printer that can print a high quality 2D bar code. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, July 07, 2011 10:49 AM To: 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Cassette Printer Recommendations I would look at Thermo, Leica and General Data printers . Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Arnold Sent: Thursday, July 07, 2011 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Cassette Printer Recommendations Hello All, We are interested in purchasing a new cassette printer and I would sincerely appreciate any recommendations for this instrument. We are particularly looking for reliability, print speed, LIS connectivity and barcode technology. Thank you very much. Arnold Rodriguez, HT (ASCP) Supervisor, Anatomic Pathology Eisenhower Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited, if you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited, if you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From JWeems <@t> sjha.org Thu Jul 7 12:05:59 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jul 7 12:06:03 2011 Subject: [Histonet] RE: Tissue Cassette Printer Recommendations In-Reply-To: <3340FC2AE9CFEE4E9D001D077700C6A21FBD7F81@NT106.info.sys> References: <3340FC2AE9CFEE4E9D001D077700C6A21FBD7F81@NT106.info.sys> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408211CB7CA@CHEXCMS10.one.ads.che.org> We have used Sakura for years now --- cassette and slide. They do a great job. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Arnold Sent: Thursday, July 07, 2011 12:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Cassette Printer Recommendations Hello All, We are interested in purchasing a new cassette printer and I would sincerely appreciate any recommendations for this instrument. We are particularly looking for reliability, print speed, LIS connectivity and barcode technology. Thank you very much. Arnold Rodriguez, HT (ASCP) Supervisor, Anatomic Pathology Eisenhower Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From christiegowan <@t> msn.com Thu Jul 7 12:21:51 2011 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Jul 7 12:21:54 2011 Subject: [Histonet] RE: Tissue Cassette Printer Recommendations In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16408211CB7CA@CHEXCMS10.one.ads.che.org> References: <3340FC2AE9CFEE4E9D001D077700C6A21FBD7F81@NT106.info.sys>, <92AD9B20A6C38C4587A9FEBE3A30E16408211CB7CA@CHEXCMS10.one.ads.che.org> Message-ID: The Sakura and the Leica are the same system as they share a patent (this is what I am told). We use the Leica IPC for our cassettes and we have the Vantage system. We did a demo of the Thermo printer but were not happy with the speed and the fact that we would need to change who we order cassettes from. We did change the color of our routine cassettes from blue to white as the white scanned better at the vantage stations. We still use blue but only for autopsy cases. We use the Vantage system at the printer to scan in the patient bar code, choose a hopper with the color we want and hit print all. So far it has worked quite well. Christie Gowan cgowan@uabmc.edu From wdesalvo.cac <@t> hotmail.com Thu Jul 7 12:36:13 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Jul 7 12:36:19 2011 Subject: [Histonet] RE: Tissue Cassette Printer Recommendations In-Reply-To: References: , <14E2C6176416974295479C64A11CB9AE0989F972@SBS2K8.premierlab.local>, <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36E5A@EXCHANGESB>, Message-ID: I agree with the previous comments and we are in the process of installing Vantage. During our testing we found that Ventana will work with any vendor, the key issue is interfacing to your LIS. As part of the process, we tested several vendor cassette printers. We chose Thermo's new Printmate 150 for a combination of reasons that worked for our process; tape transfer, speed of print, footprint (using at the grossing station) and quality of print (2D and Linear). Thermo has a 2 hopper and 6 hopper Printmate, heat transfer and prints cassettes with 2D bar code in about 8 sec General Data sells the the 1 hopper CL-01 and 12 hopper CL-12. Laser process that requires a special coated cassette that allows the Laser to etch. Prints in about 4 sec Leica sells the CL-01 and their older model IPC, inkjet and special treatment of print. Sakura sells a similar model of the Leica IPC Consider using "light" colored cassettes. The darker and red colored are more difficult to get the hand help bar code readers to recognize (red laser). Contact the vendors and I am sure they will be glad to let you demo their instrument for several days. Test them all and decide what works best in your workflow and process. William DeSalvo, B.S., HTL(ASCP) > From: JEllin@yumaregional.org > To: cbrya@lexclin.com; liz@premierlab.com; ARodriguez@emc.org; histonet@lists.utsouthwestern.edu > Date: Thu, 7 Jul 2011 17:05:11 +0000 > CC: > Subject: [Histonet] RE: Tissue Cassette Printer Recommendations > > I do not have Vantage, so I cannot comment on that. But you have to take into account the environment and the scanning quality of the print. I would also look at space, location, downtime, workflow, even clarity of barcode meaning Datamatirx, ect. There are also issues with specific cassette colors and cassette surface texture playing a part in the scanning. > > -----Original Message----- > From: Carol Bryant [mailto:cbrya@lexclin.com] > Sent: Thursday, July 07, 2011 9:55 AM > To: 'Elizabeth Chlipala'; Jesus Ellin; 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu > Subject: RE: Tissue Cassette Printer Recommendations > > I am intersted in this info also. I would like to know what printers everyone are using with the Vantage system or a bar coding system. > Thank you, > Carol > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala > Sent: Thursday, July 07, 2011 12:53 PM > To: 'Jesus Ellin'; 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Tissue Cassette Printer Recommendations > > I second Jesus's comments especially if you are moving towards bar coding. You will need a cassette printer that can print a high quality 2D bar code. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin > Sent: Thursday, July 07, 2011 10:49 AM > To: 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Tissue Cassette Printer Recommendations > > I would look at Thermo, Leica and General Data printers . > > Jesus Ellin > Yuma Regional Medical Center > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Arnold > Sent: Thursday, July 07, 2011 9:38 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Tissue Cassette Printer Recommendations > > Hello All, > > We are interested in purchasing a new cassette printer and I would > sincerely appreciate any recommendations for this instrument. We are > particularly looking for reliability, print speed, LIS connectivity and > barcode technology. > > Thank you very much. > > Arnold Rodriguez, HT (ASCP) > Supervisor, Anatomic Pathology > Eisenhower Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > DISCLAIMER: > This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited, if you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > NOTICE OF CONFIDENTIALITY > > This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. > > > > DISCLAIMER: > This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited, if you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HIMANSHU.GUPTA <@t> UCDENVER.EDU Thu Jul 7 12:37:39 2011 From: HIMANSHU.GUPTA <@t> UCDENVER.EDU (Gupta, Himanshu) Date: Thu Jul 7 12:37:44 2011 Subject: [Histonet] Request to help for rat eye histology Message-ID: <4DB7DE16B81BF74AAC37A993A79905D7010FCD3FC9BF@CORTEZ.ucdenver.pvt> Hi I need an advice from all experts there on histology of Rat eye. I need to take clear section of eye (cross section) so that I can see each layer of cells clearly. Please advise me step by step process to prepare the histology slides/sections of rat eye. I try it many times but I am not able to get the clear sections, the cells/layers got broken. Regards himanshu From christiegowan <@t> msn.com Thu Jul 7 13:05:59 2011 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Jul 7 13:06:03 2011 Subject: [Histonet] Request to help for rat eye histology In-Reply-To: <4DB7DE16B81BF74AAC37A993A79905D7010FCD3FC9BF@CORTEZ.ucdenver.pvt> References: <4DB7DE16B81BF74AAC37A993A79905D7010FCD3FC9BF@CORTEZ.ucdenver.pvt> Message-ID: Are you fixing the eyes in Davidson's? We found that using this fixative which I would have to look up since it has been so long since I did rat eyes helped maintain better morphology. > From: HIMANSHU.GUPTA@UCDENVER.EDU > To: histonet@lists.utsouthwestern.edu > Date: Thu, 7 Jul 2011 11:37:39 -0600 > Subject: [Histonet] Request to help for rat eye histology > > Hi > > I need an advice from all experts there on histology of Rat eye. > > I need to take clear section of eye (cross section) so that I can see each layer of cells clearly. Please advise me step by step process to prepare the histology slides/sections of rat eye. > > I try it many times but I am not able to get the clear sections, the cells/layers got broken. > > Regards > > himanshu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jul 7 13:06:06 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jul 7 13:06:10 2011 Subject: [Histonet] RE: Request to help for rat eye histology - long response In-Reply-To: <4DB7DE16B81BF74AAC37A993A79905D7010FCD3FC9BF@CORTEZ.ucdenver.pvt> Message-ID: <14E2C6176416974295479C64A11CB9AE0989F977@SBS2K8.premierlab.local> We process rat eyes all of the time. I know that the collection of the eyes is important and I can not comment on that since we do not collect the eyes, but anytime you are handling both mouse or rat eyes for retinal work you need to be as gentle as possible. For retinal work you need to fix in davidsons fixative (don't know how long, since we receive the eyes in 70% alcohol) formalin fixative will not work the retina will detach. One we receive the eyes which have been marked with ink as to the superior portion, we trim about 1mm or so on the nasal side (you will also need to know if the eye is right or left to help you determine which is the nasal side). I trim with a microtome blade and using a small round trimming matrix that the eye sits on top. The eyes are processed on a same day processing cycle - 20 minutes per station with proper instead of xylene. We do not process overnight. 70% - 20 minutes 80% - 20 minutes 95% - 20 minutes 100% - 20 minutes 100% - 20 minutes Propar - 20 minutes Propar - 20 minutes Propar - 20 minutes Paraffin - 20 minutes Paraffin - 20 minutes Paraffin - 20 minutes When embedding we have a deep base mold filled with melted paraffin that we drop the eye into to make sure that there are no air bubbles in the eye caviety. You can gently move the lens to get the air bubbles out if necessary, but you really do not want to manipulate the eye much since you might cause separation and tearing of the structures. Once we know that the eye has no air bubbles we place it in the appropriate base mold, we use the small square molds with the superior side of the eye towards the cassette label. We trim into the correct area, we have a microscope next to the microtome so we can make sure that we are at the optic nerve head and then place the trimmed blocks on ice for about 30 minutes or so, we then cut ribbons of 5 micron sections depending upon the study design and place on a good plus slide, let dry overnight and stain the following day with H&E. Since you are in Denver we are just in Longmont so if you want to stop by and see how we do this just e-mail me back. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gupta, Himanshu Sent: Thursday, July 07, 2011 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Request to help for rat eye histology Hi I need an advice from all experts there on histology of Rat eye. I need to take clear section of eye (cross section) so that I can see each layer of cells clearly. Please advise me step by step process to prepare the histology slides/sections of rat eye. I try it many times but I am not able to get the clear sections, the cells/layers got broken. Regards himanshu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Thu Jul 7 13:41:44 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Jul 7 13:41:53 2011 Subject: [Histonet] Bond III In-Reply-To: References: Message-ID: We have been using the Bond III for about 4 months and have starting experiencing staining on our negative control slide. We inserted and additional blocking solution and it help for the IHC stains but we are still experiencing the problem the with insitu. Has anyone experienced this problem before and how did you solve it? Thanks, Felton _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From mpence <@t> grhs.net Thu Jul 7 13:55:18 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jul 7 13:55:22 2011 Subject: [Histonet] Specimen Collection Instructions Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974C1E@is-e2k3.grhs.net> How has everyone handled this question GEN.40104 Instructions are distributed to physicians and paramedical personnel for proper collection, handling, transportation, and preparation of cytologic and tissue specimens. I feel this question is asking me to spell out to a Dr how to collect his/her specimens. To me this is like telling him/her how to practice medicine. I hope someone would like to share what you have seen that works. Thanks, Mike From efodo57 <@t> charter.net Thu Jul 7 14:12:22 2011 From: efodo57 <@t> charter.net (Elaine) Date: Thu Jul 7 14:12:25 2011 Subject: [Histonet] Specimen Collection Message-ID: <48036ac7.1499ce.1310603e2d1.Webtop.48@charter.net> You DO have to tell physicians exactly how to collect specimens. They know how to do their job but they have no idea how we do ours, even if they did a rotation thru pathology. Sometimes, you may have to tell them each time they want to send a specimen if they don't do it routinely. We still have staff that call each time they need to send a muscle bx even though they've been told numerous times and have received written instructions. Don't think of it as telling them how to do their job, but rather consider it good patient care for wanting to make sure it's done right the first time. Elaine From techmana12 <@t> yahoo.com Thu Jul 7 14:28:52 2011 From: techmana12 <@t> yahoo.com (Dorothy Glass) Date: Thu Jul 7 14:28:55 2011 Subject: [Histonet] pax2 Message-ID: <1310066932.40742.YahooMailNeo@web114512.mail.gq1.yahoo.com> Does anyone have a working protocol for pax2 on the Ventana Ultra or XT?? Can you please share on Histonet? ? Dorothy Glass Supervisor, SEPA LABS Brunswick,Ga. From akbitting <@t> geisinger.edu Thu Jul 7 14:36:38 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Jul 7 14:36:48 2011 Subject: [Histonet] pax2 In-Reply-To: <1310066932.40742.YahooMailNeo@web114512.mail.gq1.yahoo.com> References: <1310066932.40742.YahooMailNeo@web114512.mail.gq1.yahoo.com> Message-ID: <4E15D286.2B7F.00C9.1@geisinger.edu> CC1 standard, 32 min incubation with heat disabled. I use Cellmarques predilute and UltraView DAB detection. >>> Dorothy Glass 7/7/2011 3:28 PM >>> Does anyone have a working protocol for pax2 on the Ventana Ultra or XT? Can you please share on Histonet? Dorothy Glass Supervisor, SEPA LABS Brunswick,Ga. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From jo-ann.bader <@t> mcgill.ca Thu Jul 7 14:37:51 2011 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Thu Jul 7 14:38:15 2011 Subject: [Histonet] Stripped H&E's - HELP! Message-ID: <76D119EF12C904418800ED67CCB2062929A6E6CA82@EXMBXVS1.campus.mcgill.ca> Hi All, Over the past year we have had a recurring problems with our H&E's. The come out of our Gemini Stainer stripped but not on every run; we can go for months without a problem and then all of a sudden it is back again. The stripping is in the Eosin, our pathologist confirms the the hemotoxylin is not affected. There are 3 of us cutting on 3 different microtomes. The age on the microtomes range in age from 10 years to less than 1 year. Some of the solution we have tried are: We numbered all our racks to see if some of our older racks may have micro cracks in them that may retain solution which gradually dripped onto the slides. After checking for over a month there was no pattern to racks and bad staining. We tried lowering the level of the solutions to below the coated end of the slides. No results We tried changing the positions of the pots for the solutions, thinking that if there was a leak from the solutions above we would notice a difference. No luck. We checked to see if the stripes only appeared near the end of our stains life. Not that either Right now I have a technician sitting in front of the stainer as it runs to see if she can catch any abnormalities in the way the machine is transferring the slides. We rotate our alcohols every run. We change every solution after every 450 slides. I have put a call into the company to see if I can get to speak to a specialist on this machine but in the mean time I was hoping someone out there could help. Has anyone seen such a problem I, we, have run out of ideas aside from it being the machine and not being able to find out exactly how. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From kaitlin <@t> prometheushealthcare.com Thu Jul 7 14:38:07 2011 From: kaitlin <@t> prometheushealthcare.com (Kaitlin Webster) Date: Thu Jul 7 14:38:17 2011 Subject: [Histonet] HT Position in Las Vegas Message-ID: <010901cc3cdd$68a49fc0$39eddf40$@prometheushealthcare.com> Histology position in Las Vegas, Nevada with national reference lab. The position is a night shift and includes $5k sign-on bonus as well as relocation package. Pease contact me for immediate consideration. Kaitlin Webster Account Manager Prometheus Healthcare Office (301) 693-9057 Cell (407) 334-4438 Fax (301) 368-2478 Kaitlin@prometheushealthcare.com www.prometheushealthcare.com From trathborne <@t> somerset-healthcare.com Thu Jul 7 14:36:15 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Jul 7 14:38:30 2011 Subject: [Histonet] RE: Specimen Collection Instructions In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974C1E@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974C1E@is-e2k3.grhs.net> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570702F3C7@SMCMAIL01.somerset-healthcare.com> We have the basic specimen requirements (taken from our procedure manuals) from each of the Lab areas, and place them in a binder for each of the Nursing units. So whether it is a fluid or a tissue specimen, or if it requires special handling or collection, it is in this manual. If something arrives collected improperly, the unit manager is notified and a specimen incident report is generated. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, July 07, 2011 2:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Collection Instructions How has everyone handled this question GEN.40104 Instructions are distributed to physicians and paramedical personnel for proper collection, handling, transportation, and preparation of cytologic and tissue specimens. I feel this question is asking me to spell out to a Dr how to collect his/her specimens. To me this is like telling him/her how to practice medicine. I hope someone would like to share what you have seen that works. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From christiegowan <@t> msn.com Thu Jul 7 14:58:05 2011 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Jul 7 14:58:09 2011 Subject: [Histonet] RE: Specimen Collection Instructions In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570702F3C7@SMCMAIL01.somerset-healthcare.com> References: <661949901A768E4F9CC16D8AF8F2838C03974C1E@is-e2k3.grhs.net>, <3AD061FE740D464FAC7BF6B5CFB7570702F3C7@SMCMAIL01.somerset-healthcare.com> Message-ID: We have a website at our hospital that is accessible to all physicians and nursing staff for collection of all specimens. We call the website LabSource. > From: trathborne@somerset-healthcare.com > To: mpence@grhs.net; histonet@lists.utsouthwestern.edu > Date: Thu, 7 Jul 2011 19:36:15 +0000 > CC: > Subject: [Histonet] RE: Specimen Collection Instructions > > We have the basic specimen requirements (taken from our procedure manuals) from each of the Lab areas, and place them in a binder for each of the Nursing units. So whether it is a fluid or a tissue specimen, or if it requires special handling or collection, it is in this manual. If something arrives collected improperly, the unit manager is notified and a specimen incident report is generated. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Thursday, July 07, 2011 2:55 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Specimen Collection Instructions > > How has everyone handled this question GEN.40104 Instructions are distributed to physicians and paramedical personnel for proper collection, handling, transportation, and preparation of cytologic and tissue specimens. > > I feel this question is asking me to spell out to a Dr how to collect his/her specimens. To me this is like telling him/her how to practice medicine. > > I hope someone would like to share what you have seen that works. > > Thanks, Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From veronique.barres <@t> gmail.com Thu Jul 7 15:07:11 2011 From: veronique.barres <@t> gmail.com (=?ISO-8859-1?Q?V=E9ronique_Barr=E8s?=) Date: Thu Jul 7 15:07:34 2011 Subject: [Histonet] Secondary antibody for Ventana detection kits Message-ID: Hi, I would like to know if you finally optimized your staining with the anti-goat and the open secondary kit. If yes, can you tell me which antibody and dilution you used? I'm currently trying to do the same thing here. So far I only tried secondary antibodies from santa-cruz (the same that we use for western blot) without any positive result. Thanks, V?ronique Barr?s, Research Assistant Institut du cancer de Montreal Phone: 514-890-8000 ext. 24647 From ARodriguez <@t> emc.org Thu Jul 7 15:06:58 2011 From: ARodriguez <@t> emc.org (Rodriguez, Arnold) Date: Thu Jul 7 15:08:47 2011 Subject: [Histonet] RE: Tissue Cassette Printer Recommendations In-Reply-To: References: <3340FC2AE9CFEE4E9D001D077700C6A21FBD7F81@NT106.info.sys>, <92AD9B20A6C38C4587A9FEBE3A30E16408211CB7CA@CHEXCMS10.one.ads.che.org> Message-ID: <3340FC2AE9CFEE4E9D001D077700C6A21FBD7F87@NT106.info.sys> Thank you for the prompt reply. I was also told that the Sakura and Leica printers are the same. Speed of printing is close to the top of our least, so thank you for that bit of information as well. Have a wonderful day. Arnold Rodriguez, HT (ASCP) Supervisor Anatomic Pathology Eisenhower Medical Center ________________________________ From: CHRISTIE GOWAN [mailto:christiegowan@msn.com] Sent: Thursday, July 07, 2011 10:22 AM To: jweems@sjha.org; Rodriguez, Arnold; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Tissue Cassette Printer Recommendations The Sakura and the Leica are the same system as they share a patent (this is what I am told). We use the Leica IPC for our cassettes and we have the Vantage system. We did a demo of the Thermo printer but were not happy with the speed and the fact that we would need to change who we order cassettes from. We did change the color of our routine cassettes from blue to white as the white scanned better at the vantage stations. We still use blue but only for autopsy cases. We use the Vantage system at the printer to scan in the patient bar code, choose a hopper with the color we want and hit print all. So far it has worked quite well. Christie Gowan cgowan@uabmc.edu From marktarango <@t> gmail.com Thu Jul 7 15:13:12 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jul 7 15:13:18 2011 Subject: [Histonet] pax2 In-Reply-To: <4E15D286.2B7F.00C9.1@geisinger.edu> References: <1310066932.40742.YahooMailNeo@web114512.mail.gq1.yahoo.com> <4E15D286.2B7F.00C9.1@geisinger.edu> Message-ID: The only problem here is that the Cell Marque antibody is currently on backorder until at least the end of August from what I've heard. Mark On Thu, Jul 7, 2011 at 12:36 PM, Angela Bitting wrote: > CC1 standard, 32 min incubation with heat disabled. I use Cellmarques > predilute > and UltraView DAB detection. > > >>> Dorothy Glass 7/7/2011 3:28 PM >>> > Does anyone have a working protocol for pax2 on the Ventana Ultra or XT? > Can you please share on Histonet? > > Dorothy Glass > Supervisor, SEPA LABS > Brunswick,Ga. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It is > intended solely for the addressee. Access to this message by anyone else is > unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ARodriguez <@t> emc.org Thu Jul 7 15:14:11 2011 From: ARodriguez <@t> emc.org (Rodriguez, Arnold) Date: Thu Jul 7 15:15:10 2011 Subject: [Histonet] RE: Tissue Cassette Printer Recommendations In-Reply-To: References: <14E2C6176416974295479C64A11CB9AE0989F972@SBS2K8.premierlab.local> <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36E5A@EXCHANGESB> Message-ID: <3340FC2AE9CFEE4E9D001D077700C6A21FBD7F8A@NT106.info.sys> All very good suggestions and definitely things to consider. Thank you Jesus. -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Thursday, July 07, 2011 10:05 AM To: 'Carol Bryant'; 'Elizabeth Chlipala'; Rodriguez, Arnold; histonet@lists.utsouthwestern.edu Subject: RE: Tissue Cassette Printer Recommendations I do not have Vantage, so I cannot comment on that. But you have to take into account the environment and the scanning quality of the print. I would also look at space, location, downtime, workflow, even clarity of barcode meaning Datamatirx, ect. There are also issues with specific cassette colors and cassette surface texture playing a part in the scanning. -----Original Message----- From: Carol Bryant [mailto:cbrya@lexclin.com] Sent: Thursday, July 07, 2011 9:55 AM To: 'Elizabeth Chlipala'; Jesus Ellin; 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu Subject: RE: Tissue Cassette Printer Recommendations I am intersted in this info also. I would like to know what printers everyone are using with the Vantage system or a bar coding system. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, July 07, 2011 12:53 PM To: 'Jesus Ellin'; 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Cassette Printer Recommendations I second Jesus's comments especially if you are moving towards bar coding. You will need a cassette printer that can print a high quality 2D bar code. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, July 07, 2011 10:49 AM To: 'Rodriguez, Arnold'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Cassette Printer Recommendations I would look at Thermo, Leica and General Data printers . Jesus Ellin Yuma Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Arnold Sent: Thursday, July 07, 2011 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Cassette Printer Recommendations Hello All, We are interested in purchasing a new cassette printer and I would sincerely appreciate any recommendations for this instrument. We are particularly looking for reliability, print speed, LIS connectivity and barcode technology. Thank you very much. Arnold Rodriguez, HT (ASCP) Supervisor, Anatomic Pathology Eisenhower Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited, if you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited, if you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From laurie.colbert <@t> huntingtonhospital.com Thu Jul 7 15:19:28 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jul 7 15:19:32 2011 Subject: [Histonet] Stripped H&E's - HELP! In-Reply-To: <76D119EF12C904418800ED67CCB2062929A6E6CA82@EXMBXVS1.campus.mcgill.ca> References: <76D119EF12C904418800ED67CCB2062929A6E6CA82@EXMBXVS1.campus.mcgill.ca> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AE39@EXCHANGE3.huntingtonhospital.com> Make sure the level of the alcohols after the eosin covers the tops of the slides. It's most likely water dripping down the slides, and this cause the eosin on the sections to bleed so they looked striped. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Thursday, July 07, 2011 12:38 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stripped H&E's - HELP! Hi All, Over the past year we have had a recurring problems with our H&E's. The come out of our Gemini Stainer stripped but not on every run; we can go for months without a problem and then all of a sudden it is back again. The stripping is in the Eosin, our pathologist confirms the the hemotoxylin is not affected. There are 3 of us cutting on 3 different microtomes. The age on the microtomes range in age from 10 years to less than 1 year. Some of the solution we have tried are: We numbered all our racks to see if some of our older racks may have micro cracks in them that may retain solution which gradually dripped onto the slides. After checking for over a month there was no pattern to racks and bad staining. We tried lowering the level of the solutions to below the coated end of the slides. No results We tried changing the positions of the pots for the solutions, thinking that if there was a leak from the solutions above we would notice a difference. No luck. We checked to see if the stripes only appeared near the end of our stains life. Not that either Right now I have a technician sitting in front of the stainer as it runs to see if she can catch any abnormalities in the way the machine is transferring the slides. We rotate our alcohols every run. We change every solution after every 450 slides. I have put a call into the company to see if I can get to speak to a specialist on this machine but in the mean time I was hoping someone out there could help. Has anyone seen such a problem I, we, have run out of ideas aside from it being the machine and not being able to find out exactly how. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Jul 7 16:03:25 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jul 7 16:03:32 2011 Subject: [Histonet] RE: Stripped H&E's - HELP! In-Reply-To: <76D119EF12C904418800ED67CCB2062929A6E6CA82@EXMBXVS1.campus.mcgill.ca> References: <76D119EF12C904418800ED67CCB2062929A6E6CA82@EXMBXVS1.campus.mcgill.ca> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408211CB898@CHEXCMS10.one.ads.che.org> Are you sure the alcohol covers the slides well? Sounds like water running from the top of the slide. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Thursday, July 07, 2011 15:38 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stripped H&E's - HELP! Hi All, Over the past year we have had a recurring problems with our H&E's. The come out of our Gemini Stainer stripped but not on every run; we can go for months without a problem and then all of a sudden it is back again. The stripping is in the Eosin, our pathologist confirms the the hemotoxylin is not affected. There are 3 of us cutting on 3 different microtomes. The age on the microtomes range in age from 10 years to less than 1 year. Some of the solution we have tried are: We numbered all our racks to see if some of our older racks may have micro cracks in them that may retain solution which gradually dripped onto the slides. After checking for over a month there was no pattern to racks and bad staining. We tried lowering the level of the solutions to below the coated end of the slides. No results We tried changing the positions of the pots for the solutions, thinking that if there was a leak from the solutions above we would notice a difference. No luck. We checked to see if the stripes only appeared near the end of our stains life. Not that either Right now I have a technician sitting in front of the stainer as it runs to see if she can catch any abnormalities in the way the machine is transferring the slides. We rotate our alcohols every run. We change every solution after every 450 slides. I have put a call into the company to see if I can get to speak to a specialist on this machine but in the mean time I was hoping someone out there could help. Has anyone seen such a problem I, we, have run out of ideas aside from it being the machine and not being able to find out exactly how. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ltougas <@t> dawsoncollege.qc.ca Thu Jul 7 15:23:24 2011 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Thu Jul 7 17:39:30 2011 Subject: [Histonet] microtome knife holder Message-ID: <7618BC6D53F39149B7B57615C218AADF275F8672D9@EXCHANGE.ad.dawsoncollege.qc.ca> Hi again everyone, I also wanted to ask if anyone has, or new if there was ever, a regular knife (not blade) holder for the Reichert Yung 2030 microtome and/or the Leica microtome series. thank you again in advance, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College 514-931-8731, ext 1519 From ltougas <@t> dawsoncollege.qc.ca Thu Jul 7 15:17:36 2011 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Thu Jul 7 18:52:25 2011 Subject: [Histonet] low profile vs high profile blades Message-ID: <7618BC6D53F39149B7B57615C218AADF275F8672D8@EXCHANGE.ad.dawsoncollege.qc.ca> Hi everyone, Maybe because I had been using the same type all along (low profile) but I was just wondering what is the technical reason for having the two types of blades? One cuts better than the other? Price? Longer lasting? Thank you in advance for your feedback, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal, Canada From amitapandey <@t> torrentpharma.com Thu Jul 7 23:10:02 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Thu Jul 7 23:10:33 2011 Subject: [Histonet] Routine H & E stain, In-Reply-To: References: <1309960289.61746.YahooMailRC@web125417.mail.ne1.yahoo.com> Message-ID: We are using Xylene sulphur free- LR grade( from Rankem, India). I don't know about its synthetic nature but i will inquire this to manufacture. Mountant DPX from Merck . Do you have any suggestion on this combination? Thanks for all your feed back. Amita From: To: Cc: Date: 07/07/11 07:10 PM Subject: RE: [Histonet] Routine H & E stain, I have always used Harris and have not experienced this problem? I do buy mine pre-made from VWR (cat#-95057-858). I agree with the xylene comment, also what mounting media are you using? The mounting media can fade your stain over time as well. Good Luck! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amitapandey@torrentpharma.com Sent: Wednesday, July 06, 2011 11:23 PM To: Histonet; histonet-bounces@lists.utsouthwestern.edu; histonet Subject: [Histonet] Routine H & E stain, Hello Histonetters, I am from toxicopathology lab where we perform H&E on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result is good (Purplish pink look stained slide) on all tissue, but our observation is after 5-6 months time this get faded and become towards pinkish type though we can observe the slide. We want to retain its fresh stained color. Please suggest me how to keep stable stain for longer period or do you suggest to switch to any other type of haematoxylin - prepared or commercially available? Looking forward for your feed back. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfonner <@t> labpath.com Fri Jul 8 04:51:29 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Fri Jul 8 04:55:12 2011 Subject: [Histonet] Stripped H&E's - HELP! In-Reply-To: <76D119EF12C904418800ED67CCB2062929A6E6CA82@EXMBXVS1.campus.mcgill.ca> References: <76D119EF12C904418800ED67CCB2062929A6E6CA82@EXMBXVS1.campus.mcgill.ca> Message-ID: <000301cc3d54$9c980f20$d5c82d60$@com> Jo-Ann, I have seen this problem many times over the years. Nearly 100% of the time it is due to the level of the alcohol after the eosin being too low. Whether you water rinse or go straight into a 95% etoh, the level of your first alcohol after eosin MUST be covering your entire slide. If not, the eosin will slowly drip down the slides in the subsequent solutions. This can also happen after hematoxylin. Keep the hematoxylin and eosin LOWER than the solutions that come after them. Hope this helps. Regards, Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Thursday, July 07, 2011 3:38 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stripped H&E's - HELP! Hi All, Over the past year we have had a recurring problems with our H&E's. The come out of our Gemini Stainer stripped but not on every run; we can go for months without a problem and then all of a sudden it is back again. The stripping is in the Eosin, our pathologist confirms the the hemotoxylin is not affected. There are 3 of us cutting on 3 different microtomes. The age on the microtomes range in age from 10 years to less than 1 year. Some of the solution we have tried are: We numbered all our racks to see if some of our older racks may have micro cracks in them that may retain solution which gradually dripped onto the slides. After checking for over a month there was no pattern to racks and bad staining. We tried lowering the level of the solutions to below the coated end of the slides. No results We tried changing the positions of the pots for the solutions, thinking that if there was a leak from the solutions above we would notice a difference. No luck. We checked to see if the stripes only appeared near the end of our stains life. Not that either Right now I have a technician sitting in front of the stainer as it runs to see if she can catch any abnormalities in the way the machine is transferring the slides. We rotate our alcohols every run. We change every solution after every 450 slides. I have put a call into the company to see if I can get to speak to a specialist on this machine but in the mean time I was hoping someone out there could help. Has anyone seen such a problem I, we, have run out of ideas aside from it being the machine and not being able to find out exactly how. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leticia.figliuolo <@t> roche.com Fri Jul 8 07:33:38 2011 From: leticia.figliuolo <@t> roche.com (Figliuolo, Leticia) Date: Fri Jul 8 07:33:45 2011 Subject: [Histonet] PAS in frozen sections Message-ID: <069E6CF048B915488720412DAFD1474D03FC2A5901@RNUMSEM702.nala.roche.com> Does anyone have a procedure for doing a PAS on frozen tissue?? For how long should I fix the slides?... Do I have to shorten the times in the regents? Any tips/tricks would be greatly appreciated. We are staining rat lung tissue. Thank you, Leticia Figliuolo e-mail: leticia.figliuolo@roche.com From Wanda.Smith <@t> HCAhealthcare.com Fri Jul 8 07:52:26 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Jul 8 07:52:33 2011 Subject: [Histonet] RE: Specimen Collection Instructions In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974C1E@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974C1E@is-e2k3.grhs.net> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA261DE61670@NADCWPMSGCMS03.hca.corpad.net> Mike, Pathology and Lab print a collection manual every few years with the requirements for blood test (tubes, amounts, etc) and collection requirements for cytology and tissue specimens (fresh, frozen sections, fresh fluids). We also have the manual on-line and we give the physician offices access to this on-line manual if they have any questions. It seems to work well for us. The on-line manual is best because if you make changes, you don't have to reprint the whole book. Hope this helps!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, July 07, 2011 2:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Collection Instructions How has everyone handled this question GEN.40104 Instructions are distributed to physicians and paramedical personnel for proper collection, handling, transportation, and preparation of cytologic and tissue specimens. I feel this question is asking me to spell out to a Dr how to collect his/her specimens. To me this is like telling him/her how to practice medicine. I hope someone would like to share what you have seen that works. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shehnazster <@t> gmail.com Fri Jul 8 08:29:54 2011 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Fri Jul 8 08:29:58 2011 Subject: [Histonet] safe handling of tissue for histology containing radionuclides In-Reply-To: References: Message-ID: Could someone please shed some light. Hi Everyone, Could someone kindly shed some light on safe handling of tissue for histology containing radionuclides - to comply with CAP standards. How is this done? What should I include in the policy / work instruction? Thanks in advance S Kahn From talulahgosh <@t> gmail.com Fri Jul 8 09:01:11 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jul 8 09:01:16 2011 Subject: [Histonet] microtome knife holder In-Reply-To: <7618BC6D53F39149B7B57615C218AADF275F8672D9@EXCHANGE.ad.dawsoncollege.qc.ca> References: <7618BC6D53F39149B7B57615C218AADF275F8672D9@EXCHANGE.ad.dawsoncollege.qc.ca> Message-ID: All of the Leica cryostats have knife holders, I would contact Leica to ask as their website is terrible. You could also just google it and I bet some other company sells them. The other one, I'm not sure of. We don't use disposable blades so we've never had a knife holder for that. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Thu, Jul 7, 2011 at 4:23 PM, Liette Tougas wrote: > Hi again everyone, > > I also wanted to ask if anyone has, or new if there was ever, a regular > knife (not blade) holder for the Reichert Yung 2030 microtome and/or the > Leica microtome series. > > thank you again in advance, > > Liette Tougas, RT, B.Sc., M.Sc. > Biomedical Laboratory Technology Department > Dawson College > 514-931-8731, ext 1519 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Jul 8 09:01:47 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 8 09:01:50 2011 Subject: [Histonet] low profile vs high profile blades In-Reply-To: <7618BC6D53F39149B7B57615C218AADF275F8672D8@EXCHANGE.ad.dawsoncollege.qc.ca> Message-ID: <1310133707.93653.YahooMailClassic@web65704.mail.ac4.yahoo.com> It depends on the embedded tissue. For harder tissues high profile are more suitable. Ren? J. --- On Thu, 7/7/11, Liette Tougas wrote: From: Liette Tougas Subject: [Histonet] low profile vs high profile blades To: "histonet@lists.utsouthwestern.edu" Date: Thursday, July 7, 2011, 4:17 PM Hi everyone, Maybe because I had been using the same type all along (low profile) but I was just wondering what is the technical reason for having the two types of blades?? One cuts better than the other? Price? Longer lasting? Thank you in advance for your feedback, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jul 8 09:03:53 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 8 09:03:57 2011 Subject: [Histonet] microtome knife holder In-Reply-To: <7618BC6D53F39149B7B57615C218AADF275F8672D9@EXCHANGE.ad.dawsoncollege.qc.ca> Message-ID: <1310133833.32667.YahooMailClassic@web65709.mail.ac4.yahoo.com> Knives (not disposable blades) do not need holders. They are used by themselves. Ren? J. --- On Thu, 7/7/11, Liette Tougas wrote: From: Liette Tougas Subject: [Histonet] microtome knife holder To: "histonet@lists.utsouthwestern.edu" Date: Thursday, July 7, 2011, 4:23 PM Hi again everyone, I also wanted to ask if anyone has, or new if there was ever, a regular knife (not blade) holder for the Reichert Yung 2030 microtome and/or the Leica microtome series. thank you again in advance, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College 514-931-8731, ext 1519 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Fri Jul 8 09:17:57 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Jul 8 09:18:04 2011 Subject: [Histonet] safe handling of tissue for histology containingradionuclides In-Reply-To: References: Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D7010A1F30@usctmx1176.merck.com> My lab work with tissues containing radionuclides fairly often, but since we are in industry and not under CAP regs I can only give you some general info. It depends on the specific radionuclide and thus it's half-life. Half-lifes for common SPECT isotopes are: I-125 60 days Tc-99m 6 hours In-111 2.8 days Ga-67 3.26 days for common PET isotopes: F-18 2 hours C-11 20 min. Cu-64 12.7 hours Ga- 68 1.13 hours Others you may encounter: H-3 12 years P-32 14.3 days P-33 25.4 days S-35 87.44 days C-14 5730 years We have designated areas in the lab for working with radioactivity and designated cryostats. We only process tissue with short half-lives (F-18, C-11, Cu-64, Tc99m, Ga-68) to paraffin, and before doing so we wait 10 half-lives before processing. We all wear personal dosimetry when handling tissues containing radionuclides and that dosimetry is monitored monthly for our exposure. All radioactive waste, including disposable PPE, is collected in special waste containers for proper disposal - absolutely nothing down the sink or in regular trash. Labs areas are surveyed with the appropriate meters/probes depending on the radionuclide, and wipe tests are performed and wipes counted with a scintillation counter. That scratches the surface - others can chime in Best regards, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shehnaz khan Sent: Friday, July 08, 2011 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] safe handling of tissue for histology containingradionuclides Could someone please shed some light. Hi Everyone, Could someone kindly shed some light on safe handling of tissue for histology containing radionuclides - to comply with CAP standards. How is this done? What should I include in the policy / work instruction? Thanks in advance S Kahn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From brett_connolly <@t> merck.com Fri Jul 8 09:21:10 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Jul 8 09:21:14 2011 Subject: [Histonet] microtome knife holder In-Reply-To: <1310133833.32667.YahooMailClassic@web65709.mail.ac4.yahoo.com> References: <7618BC6D53F39149B7B57615C218AADF275F8672D9@EXCHANGE.ad.dawsoncollege.qc.ca> <1310133833.32667.YahooMailClassic@web65709.mail.ac4.yahoo.com> Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D7010A1F32@usctmx1176.merck.com> Um, actually steel knives do in fact require a holder and disposable blades require a different holder, we have both from Leica. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, July 08, 2011 10:04 AM To: histonet@lists.utsouthwestern.edu; Liette Tougas Subject: Re: [Histonet] microtome knife holder Knives (not disposable blades) do not need holders. They are used by themselves. Ren? J. --- On Thu, 7/7/11, Liette Tougas wrote: From: Liette Tougas Subject: [Histonet] microtome knife holder To: "histonet@lists.utsouthwestern.edu" Date: Thursday, July 7, 2011, 4:23 PM Hi again everyone, I also wanted to ask if anyone has, or new if there was ever, a regular knife (not blade) holder for the Reichert Yung 2030 microtome and/or the Leica microtome series. thank you again in advance, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College 514-931-8731, ext 1519 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From flnails <@t> texaschildrens.org Fri Jul 8 11:26:01 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Jul 8 11:26:11 2011 Subject: [Histonet] HM525 Cryostat In-Reply-To: References: <1310066932.40742.YahooMailNeo@web114512.mail.gq1.yahoo.com> <4E15D286.2B7F.00C9.1@geisinger.edu> Message-ID: I purchased a new Microm HM525 cryostat about 3 months ago. The unit overheated causing some of the internal parts to warp. Has anyone else experienced this or similar issue? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From plucas <@t> biopath.org Fri Jul 8 11:44:51 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Jul 8 11:39:39 2011 Subject: [Histonet] Embedding Media Message-ID: <9251EDA88CA748D6861A0C3BB0E32FCB@biopath.local> Hello, We are considering a switch to a different brand of paraffin and this is because I feel we are having too many compressions in some of our tissue sections. Currently, we use Tissue Path Paraplast, regular. I would like to get feedback from you as to what you prefer. Looking on line, Richard Allan has a product called Signature Series Paraffin that comes in a type L that offers compression-free sections and I was also hoping to get any feedback on that product. I would greatly appreciate any suggestions and thoughts. Thanks in advance, Paula From efodo57 <@t> charter.net Fri Jul 8 12:15:16 2011 From: efodo57 <@t> charter.net (Elaine) Date: Fri Jul 8 12:15:19 2011 Subject: [Histonet] Embedding Media Message-ID: <5a08812c.18b859.1310abf076e.Webtop.45@charter.net> We use Paraplast Plus. It's a little 'sticky" but works very well. From thiggins <@t> cddmedical.com Fri Jul 8 12:59:29 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Fri Jul 8 12:59:33 2011 Subject: [Histonet] Re: Embedding Media References: <20110708170448.4BD7E12CCF7E@barracuda.crvinc.net> Message-ID: <001c01cc3d98$c8b61040$e001a8c0@cdd.loc> Hi Paula, Obviously there are no "compression free" paraffin's on the market. Look for a paraffin with a higher polymer content, this makes the paraffin firmer and less likely to compress. Other factor for into section compression but paraffin is a good place to start. The higher polymer paraffin's are not as suited for processing, it is more for the embedding portion of the process. Use a paraffin with lower polymers content in the processor and higher for embedding and you will like the results. Tim Date: Fri, 8 Jul 2011 09:44:51 -0700 From: "Paula Lucas" Subject: [Histonet] Embedding Media To: Message-ID: <9251EDA88CA748D6861A0C3BB0E32FCB@biopath.local> Content-Type: text/plain; charset="us-ascii" Hello, We are considering a switch to a different brand of paraffin and this is because I feel we are having too many compressions in some of our tissue sections. Currently, we use Tissue Path Paraplast, regular. I would like to get feedback from you as to what you prefer. Looking on line, Richard Allan has a product called Signature Series Paraffin that comes in a type L that offers compression-free sections and I was also hoping to get any feedback on that product. I would greatly appreciate any suggestions and thoughts. Thanks in advance, Paula From ross <@t> premierlab.com Fri Jul 8 13:07:53 2011 From: ross <@t> premierlab.com (Ross Benik) Date: Fri Jul 8 13:07:57 2011 Subject: [Histonet] Direct Mouse IgG Message-ID: <14E2C6176416974295479C64A11CB9AE098C6444@SBS2K8.premierlab.local> Hello Histonet, Does anyone have a working protocol that stains directly for mouse IgG (fluorescent) on frozen sections? We are currently staining with a goat anti mouse IgG 555 (highly cross absorbed) and we have having a hard time deciding what is specific staining and what is no. Information such as micron thickness, method of fixation (acetone/methanol, NBF etc) and staining parameters would be great! Thanks, Ross From Marcia_Gaiser <@t> ssmhc.com Fri Jul 8 13:30:13 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Fri Jul 8 13:30:22 2011 Subject: [Histonet] HT Supervisor Position in Oklahoma City OK Message-ID: <728F817C02110E498D803A7C3B0C6248068D43B5B0@S009-APEXM06.ds.ad.ssmhc.com> POSITION SUMMARY: Plan, coordinate, and supervise the functions and activities of personnel in the histology section of the pathology laboratory. Ensure the validity and accuracy of the test results. POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, AND BONDING: Certified as an HT or HLT by the American Society of Clinical Pathology (ASCP) or other nationally recognized certifying agency acceptable to the Laboratory Director; or experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. Supervisory experience preferred. Apply online at www.saintsok.com, Ad# 11522. Thank you! Anna King HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax [https://mobile.ssmhc.com/owa/attachment.ashx?id=RgAAAABMsXdcX9YUQLcxZSN9Shu5BwByj4F8AhEOSY2AOnw7DGJIAAUpEpPHAAByj4F8AhEOSY2AOnw7DGJIAAaNIGfyAAAJ&attcnt=1&attid0=EAC4fHC6%2fw7NSKW36Sa%2f%2bEMD] Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From thiggins <@t> cddmedical.com Fri Jul 8 13:47:51 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Fri Jul 8 13:47:54 2011 Subject: [Histonet] Tissue controls Message-ID: <003301cc3d9f$8addd530$e001a8c0@cdd.loc> I have an abundance of H-pylori controls that I would be willing to part with and a number others as well. Thanks, Tim From efodo57 <@t> charter.net Fri Jul 8 14:49:54 2011 From: efodo57 <@t> charter.net (Elaine) Date: Fri Jul 8 14:49:57 2011 Subject: [Histonet] Histonet] Tissue controls Message-ID: <359c0bfc.155f47.1310b4c986b.Webtop.48@charter.net> Would you happen to have any AFB, fungus or copper controls?? Elaine From histotech <@t> imagesbyhopper.com Fri Jul 8 14:56:48 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Jul 8 15:02:17 2011 Subject: [Histonet] Embedding Media In-Reply-To: <9251EDA88CA748D6861A0C3BB0E32FCB@biopath.local> References: <9251EDA88CA748D6861A0C3BB0E32FCB@biopath.local> Message-ID: We use the infiltration & embedding paraffin from Surgipath/Leica and are very happy with it. Michelle Sent from my iPhone On Jul 8, 2011, at 12:44 PM, "Paula Lucas" wrote: > Hello, > > > > We are considering a switch to a different brand of paraffin and this is > because I feel we are having too many compressions in some of our tissue > sections. Currently, we use Tissue Path Paraplast, regular. > > > > I would like to get feedback from you as to what you prefer. Looking on > line, Richard Allan has a product called Signature Series Paraffin that > comes in a type L that offers compression-free sections and I was also > hoping to get any feedback on that product. > > > > I would greatly appreciate any suggestions and thoughts. > > Thanks in advance, > > Paula > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From efodo57 <@t> charter.net Fri Jul 8 15:26:40 2011 From: efodo57 <@t> charter.net (Elaine) Date: Fri Jul 8 15:26:44 2011 Subject: [Histonet] Histonet] Tissue controls Message-ID: <41b4853e.1566e5.1310b6e410c.Webtop.48@charter.net> Well rotting meat is a new one for me!! We do purchase our AFB from them, we just do so many it would be more cost effective to have our own blocks. Thanks. From sadey <@t> hotmail.ca Sat Jul 9 08:31:52 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sat Jul 9 08:31:56 2011 Subject: [Histonet] Number of blocks before a processor change??? Message-ID: Hello: We currently change our processors every 5 runs. I am thinking it would be more cost and time effective to count the number of blocks before doing a change. If you count your blocks could you share with me at what number you do a change. We run all types of tissues. We use a gallon of reagent in each position. Vip5. Thanks in advance. :) Sheila From sadey <@t> hotmail.ca Sat Jul 9 08:36:12 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sat Jul 9 08:36:16 2011 Subject: [Histonet] prostate bx containers Message-ID: Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila From rjbuesa <@t> yahoo.com Sat Jul 9 09:36:36 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 9 09:36:41 2011 Subject: [Histonet] Number of blocks before a processor change??? In-Reply-To: Message-ID: <1310222196.80179.YahooMailClassic@web65710.mail.ac4.yahoo.com> I always counted the number of blocks processed per run. When my VIP reached its maximum (e.g. 300 blocks), I changed all the alcohols of less than 100%, and moved "down" the 100EthOL, the xylenes and the paraffins (meaning eliminating the first 100 EthOL, xylene and paraffin, and replacing the last). Ren? J. --- On Sat, 7/9/11, Sheila Adey wrote: From: Sheila Adey Subject: [Histonet] Number of blocks before a processor change??? To: histonet@lists.utsouthwestern.edu Date: Saturday, July 9, 2011, 9:31 AM Hello: We currently change our processors every 5 runs. I am thinking it would be more cost and time effective to count the number of blocks before doing a change. If you count your blocks could you share with me at what number you do a change. We run all types of tissues. We use a gallon of reagent in each position. Vip5. Thanks in advance. :) Sheila ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shehnazster <@t> gmail.com Sat Jul 9 10:56:42 2011 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Sat Jul 9 10:56:47 2011 Subject: [Histonet] Many thanks: radionuclides query Message-ID: Hi All, Sincere thanks to all who responded to my query on tissue containing radionuclides and CAP - especially Galbraith Joe, Connolly Bret and Dawn. Apologies if I missed out anyone else. Joyce Fortin: I have forwarded all of these replies to you. Best Wishes Shehnaz Kahn From pvalente <@t> sbcglobal.net Sat Jul 9 12:44:34 2011 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Sat Jul 9 12:44:37 2011 Subject: [Histonet] Number of blocks before a processor change??? In-Reply-To: References: Message-ID: <1310233474.14733.YahooMailRC@web81704.mail.mud.yahoo.com> We rotate one of each solution type(including wax) every 50 blocks or every 2 runs(whichever occurs first). Helps counteract carryover from sponges and seems to work better and conserve a little on reagents than when doing complete change less frequently. Note: we rune a large number of small biopsies using sponges. Pat Valente Histo Manager Healthtronics San Antonio ________________________________ From: Sheila Adey To: histonet@lists.utsouthwestern.edu Sent: Sat, July 9, 2011 8:31:52 AM Subject: [Histonet] Number of blocks before a processor change??? Hello: We currently change our processors every 5 runs. I am thinking it would be more cost and time effective to count the number of blocks before doing a change. If you count your blocks could you share with me at what number you do a change. We run all types of tissues. We use a gallon of reagent in each position. Vip5. Thanks in advance. :) Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From t.dettmering <@t> gsi.de Sat Jul 9 12:52:12 2011 From: t.dettmering <@t> gsi.de (Till Dettmering) Date: Sat Jul 9 12:52:19 2011 Subject: [Histonet] Fluorescent Label for Collagen-1 Message-ID: <09C7280F-1C2F-4894-8063-F058F000B132@gsi.de> Dear Histonetters, I'm performing fluorescent microscopy on PFA-fixed, paraffin-embedded rat tissue. I need to stain Collagen with a fluorescent label. I was trying a goat-anti-hu/ms/rat-Col1A Ab from Santa Cruz (sc-25974) but I simply can't see a signal. My question would be whether one of you can recommend either an antibody which actually works for rat tissue or an alternative fluorescent probe I could use. I recently read about the Collagen-binding protein CNA35, which looks very promising, but I don't have the means of labeling this protein for myself and I couldn't find a vendor for a labeled version of CNA35. I'd be glad to hear your thoughts and ideas on this topic! All the best, Till From TMcNemar <@t> lmhealth.org Sun Jul 10 09:53:32 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Sun Jul 10 09:53:40 2011 Subject: [Histonet] In search of a cryostat... Message-ID: Hello all, I am looking to budget for a new cryostat and was wondering what others are using. I would like one that is safe, well lit, roomy, and has some sort of on-board decontamination routine. All input appreciated. Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From rjbuesa <@t> yahoo.com Sun Jul 10 10:55:47 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jul 10 10:55:52 2011 Subject: [Histonet] In search of a cryostat... In-Reply-To: Message-ID: <1310313347.81123.YahooMailClassic@web65713.mail.ac4.yahoo.com> Buy Leica Ren? J. --- On Sun, 7/10/11, Tom McNemar wrote: From: Tom McNemar Subject: [Histonet] In search of a cryostat... To: "histonet@lists.utsouthwestern.edu" Date: Sunday, July 10, 2011, 10:53 AM Hello all, I am looking to budget for a new cryostat and was wondering what others are using.? I would like one that is safe, well lit, roomy, and has some sort of on-board decontamination routine.? All input appreciated.? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Sun Jul 10 14:29:56 2011 From: member <@t> linkedin.com (Cesar Romero via LinkedIn) Date: Sun Jul 10 14:29:59 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <2135085559.5396297.1310326196303.JavaMail.app@ela4-bed78.prod> LinkedIn ------------ Cesar Romero requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Cesar Accept invitation from Cesar Romero http://www.linkedin.com/e/yvpgd1-gpyedvt7-51/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I130293311_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYNcjcPej8McP59bQNMmDtTd5h9bPsQc3gPe3sUd3cLrCBxbOYWrSlI/EML_comm_afe/ View invitation from Cesar Romero http://www.linkedin.com/e/yvpgd1-gpyedvt7-51/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I130293311_13/3cNnP4NcPcVcz0PckALqnpPbOYWrSlI/svi/ ------------------------------------------ Why might connecting with Cesar Romero be a good idea? People Cesar Romero knows can discover your profile: Connecting to Cesar Romero will attract the attention of LinkedIn users. See who's been viewing your profile: http://www.linkedin.com/e/yvpgd1-gpyedvt7-51/wvp/inv18_wvmp/ -- (c) 2011, LinkedIn Corporation From amitapandey <@t> torrentpharma.com Mon Jul 11 01:07:47 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Mon Jul 11 01:08:24 2011 Subject: [Histonet] Re: Embedding Media In-Reply-To: <001c01cc3d98$c8b61040$e001a8c0@cdd.loc> References: <20110708170448.4BD7E12CCF7E@barracuda.crvinc.net> <001c01cc3d98$c8b61040$e001a8c0@cdd.loc> Message-ID: I continuation of same discussion , i would like to have clarification for our lab..we use ....beaded paraffin "Leica Paraplast" both for processing and embedding . Do you advice us to use different paraffin for both steps? Paula, Please share if you get any feed back on Richard Allan product. Amita From: "Tim Higgins" To: Date: 07/08/2011 11:34 PM Subject: [Histonet] Re: Embedding Media Sent by: histonet-bounces@lists.utsouthwestern.edu Hi Paula, Obviously there are no "compression free" paraffin's on the market. Look for a paraffin with a higher polymer content, this makes the paraffin firmer and less likely to compress. Other factor for into section compression but paraffin is a good place to start. The higher polymer paraffin's are not as suited for processing, it is more for the embedding portion of the process. Use a paraffin with lower polymers content in the processor and higher for embedding and you will like the results. Tim Date: Fri, 8 Jul 2011 09:44:51 -0700 From: "Paula Lucas" Subject: [Histonet] Embedding Media To: Message-ID: <9251EDA88CA748D6861A0C3BB0E32FCB@biopath.local> Content-Type: text/plain; charset="us-ascii" Hello, We are considering a switch to a different brand of paraffin and this is because I feel we are having too many compressions in some of our tissue sections. Currently, we use Tissue Path Paraplast, regular. I would like to get feedback from you as to what you prefer. Looking on line, Richard Allan has a product called Signature Series Paraffin that comes in a type L that offers compression-free sections and I was also hoping to get any feedback on that product. I would greatly appreciate any suggestions and thoughts. Thanks in advance, Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Mon Jul 11 04:22:22 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Jul 11 04:22:37 2011 Subject: [Histonet] Waste disposal Message-ID: For those who use the Ventana IHC and special stainers, how do you dispose of the liquid waste that is generated? Thanks! From histotech <@t> imagesbyhopper.com Mon Jul 11 05:44:43 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Jul 11 05:44:52 2011 Subject: [Histonet] Re: Embedding Media In-Reply-To: References: <20110708170448.4BD7E12CCF7E@barracuda.crvinc.net> <001c01cc3d98$c8b61040$e001a8c0@cdd.loc> Message-ID: <02572341-7224-41E3-A831-E00185FD690E@imagesbyhopper.com> Amita, The paraffin that we use is also a Leica product. It was formerly sold under the Surgipath label, but since to companies have merged together, it now sells under the Leica name. It is a "beaded" pellet paraffin and has a version suitable for infiltration and a version suitable for embedding. The box of the embedding paraffin suggests it can be utilized for *both* purposes, however we do not choose to use it that way. So yes, we use two different types of paraffin. Hope that helps! Michelle On Jul 11, 2011, at 2:07 AM, amitapandey@torrentpharma.com wrote: > I continuation of same discussion , i would like to have clarification for > our lab..we use ....beaded paraffin "Leica Paraplast" both for processing > and embedding . Do you advice us to use different paraffin for both steps? > > > Paula, Please share if you get any feed back on Richard Allan product. > > Amita > > > From: "Tim Higgins" > To: > Date: 07/08/2011 11:34 PM > Subject: [Histonet] Re: Embedding Media > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > Hi Paula, > > Obviously there are no "compression free" paraffin's on the market. Look > for a paraffin with a higher polymer content, this makes the paraffin > firmer > and less likely to compress. Other factor for into section compression > but > paraffin is a good place to start. > > The higher polymer paraffin's are not as suited for processing, it is more > for the embedding portion of the process. > > Use a paraffin with lower polymers content in the processor and higher for > embedding and you will like the results. > > > Tim > > > Date: Fri, 8 Jul 2011 09:44:51 -0700 > From: "Paula Lucas" > Subject: [Histonet] Embedding Media > To: > Message-ID: <9251EDA88CA748D6861A0C3BB0E32FCB@biopath.local> > Content-Type: text/plain; charset="us-ascii" > > Hello, > > > > We are considering a switch to a different brand of paraffin and this is > because I feel we are having too many compressions in some of our tissue > sections. Currently, we use Tissue Path Paraplast, regular. > > > > I would like to get feedback from you as to what you prefer. Looking on > line, Richard Allan has a product called Signature Series Paraffin that > comes in a type L that offers compression-free sections and I was also > hoping to get any feedback on that product. > > > > I would greatly appreciate any suggestions and thoughts. > > Thanks in advance, > > Paula > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Saro.Bascaramurty <@t> nrc-cnrc.gc.ca Mon Jul 11 10:40:06 2011 From: Saro.Bascaramurty <@t> nrc-cnrc.gc.ca (Bascaramurty, Saro) Date: Mon Jul 11 10:40:11 2011 Subject: [Histonet] Written SOP Message-ID: Hi, I am wondering if anyone of you have a written SOP on 'Cryosectioning of animal tissues implanted with human cells that are totally not screened for Hep B, HEP C, HIV, Mycobacterium tuberculosis and other adventitious agents' . If you have one written, would you be kind enough to share with me? Thanks in advance! Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramurty@nrc-cnrc.gc.ca From cpyse <@t> x-celllab.com Mon Jul 11 10:41:04 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Mon Jul 11 10:40:59 2011 Subject: [Histonet] water Message-ID: <000c01cc3fe0$f1a10e70$d4e32b50$@com> Hi Histonetters I have 2 tech off on vacation so I thought I would take the easy way this time. Does anyone know the difference between distilled water system and the reverse osmosis water system? Could if effect the IHC staining by using one or the other? Usually I would research it myself but it has be crazy around here lately. Thanks in advance for any help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext.232 e-mail cpyse@x-celllab.com From rjbuesa <@t> yahoo.com Mon Jul 11 10:45:35 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 11 10:45:43 2011 Subject: [Histonet] Re: Embedding Media In-Reply-To: Message-ID: <1310399135.64444.YahooMailClassic@web65702.mail.ac4.yahoo.com> In spite of many opinions on the contrary, I always found an error to use two different paraffins: = one for INFILTRATION and the other for EMBEDDING. You have to consider that, theoretical this is a mistake, because the paraffin that has infiltrated the tissue and REMAINS inside it sustaining its microscopic architecture should be the same, with the same resistance to cutting, than the embedding paraffin. The thing is that you have to select a paraffin adequate for your subjects. When many years ago I used to work with plant material I always used a high melting point paraffin (63-65?C from Merck), a lower melting point (53-57?C) for adult animal tissue, and a softer paraffin (45-50?C) for embryonic material. Having a tissue infiltrated with a paraffin with a certain melting point (resistance) surrounded by ANOTHER paraffin with a different melting point (either higher or lower, but usually higher) is a mistake. Select a paraffin adequate for your subject and use it for both the infiltration and the embedding. Ren? J. --- On Mon, 7/11/11, amitapandey@torrentpharma.com wrote: From: amitapandey@torrentpharma.com Subject: Re: [Histonet] Re: Embedding Media To: "Tim Higgins" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Monday, July 11, 2011, 2:07 AM I continuation of same discussion , i would like to have clarification for our lab..we use ....beaded paraffin "Leica Paraplast"? both for processing and embedding . Do you advice us to use different paraffin for both steps? Paula, Please share if you get any feed back on Richard Allan product. Amita From:???"Tim Higgins" To:? ??? Date:???07/08/2011 11:34 PM Subject:? ? ? ? [Histonet] Re: Embedding Media Sent by:? ? ? ? histonet-bounces@lists.utsouthwestern.edu Hi Paula, Obviously there are no "compression free" paraffin's on the market.? Look for a paraffin with a higher polymer content, this makes the paraffin firmer and less likely to compress.? Other factor for into section compression but paraffin is a good place to start. The higher polymer paraffin's are not as suited for processing, it is more for the embedding portion of the process. Use a paraffin with lower polymers content in the processor and higher for embedding and you will like the results. Tim Date: Fri, 8 Jul 2011 09:44:51 -0700 From: "Paula Lucas" Subject: [Histonet] Embedding Media To: Message-ID: <9251EDA88CA748D6861A0C3BB0E32FCB@biopath.local> Content-Type: text/plain; charset="us-ascii" Hello, We are considering a switch to a different brand of paraffin and this is because I feel we are having too many compressions in some of our tissue sections.? Currently, we use Tissue Path Paraplast, regular. I would like to get feedback from you as to what you prefer.? Looking on line, Richard Allan has a product called Signature Series Paraffin that comes in a type L that offers compression-free sections and I was also hoping to get any feedback on that product. I would greatly appreciate any suggestions and thoughts. Thanks in advance, Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> wvhcs.org Mon Jul 11 10:44:52 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Mon Jul 11 10:45:49 2011 Subject: [Histonet] prostate bx containers References: Message-ID: We don't currently use them but I've used them in the past. As long as you don't 'bump' them on the grossing station while they're open, they're fine. I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ________________________________ From: Sheila Adey [mailto:sadey@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From rjbuesa <@t> yahoo.com Mon Jul 11 10:50:28 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 11 10:50:33 2011 Subject: [Histonet] Written SOP In-Reply-To: Message-ID: <1310399428.42173.YahooMailClassic@web65705.mail.ac4.yahoo.com> Really? Do you think that anybody is going to have an SOP for such an specific and narrow subject? Please remember that an SOP procedure is nothing other than the description of the method you use in a way that anybody else can follow it, and do the same thing always. I think that you will be better of if you describe how do you complete such an assignment. Ren? K. --- On Mon, 7/11/11, Bascaramurty, Saro wrote: From: Bascaramurty, Saro Subject: [Histonet] Written SOP To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 11, 2011, 11:40 AM Hi, I am wondering if anyone of you have a written SOP on 'Cryosectioning of animal tissues implanted with human cells that are totally not screened for Hep B, HEP C, HIV, Mycobacterium tuberculosis and other adventitious agents' . If you have one written, would you be kind enough to share with me? Thanks in advance! Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramurty@nrc-cnrc.gc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arme <@t> optonline.net Mon Jul 11 10:52:27 2011 From: arme <@t> optonline.net (American Resource Medical) Date: Mon Jul 11 10:52:35 2011 Subject: [Histonet] For Sale - Mopec MB100 Countertop Grossing Station Message-ID: <006701cc3fe2$8930fe70$9b92fb50$@net> For Sale - Mopec MB100 Countertop Grossing Station This is a used piece in excellent condition and half the price of new. http://media.mopec.com/media/pdf/Catalog2007smaller159.pdf This is a countertop unit. American ReSource Medical P: 201.833.1550 From rjbuesa <@t> yahoo.com Mon Jul 11 10:55:29 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 11 10:55:33 2011 Subject: [Histonet] water In-Reply-To: <000c01cc3fe0$f1a10e70$d4e32b50$@com> Message-ID: <1310399729.34085.YahooMailClassic@web65716.mail.ac4.yahoo.com> Distilled water is nothing more than water without salts or ions. How do you get to that is irrelevant as long as the water, at the end, is "pure". Now days it is frequent to distinguish between "distilled" (meaning that the water first was evaporated?using a heat source?and later was condensated by removing the heat from the water vapor) and "deionized" (meaning that the ions were removed chemically), but the final product ("pure" water) is the same and completely interchangeable for IHC or any other purpose. Ren? J. --- On Mon, 7/11/11, Cynthia Pyse wrote: From: Cynthia Pyse Subject: [Histonet] water To: histonet@lists.utsouthwestern.edu Date: Monday, July 11, 2011, 11:41 AM Hi Histonetters I have 2 tech off on vacation so I thought I would take the easy way this time. Does anyone know the difference between distilled water system and the reverse osmosis water system? Could if effect the IHC staining by using one or the other? Usually I would research it myself but it has be crazy around here lately. Thanks in advance for any help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext.232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Jul 11 11:00:11 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Jul 11 11:00:18 2011 Subject: [Histonet] Written SOP In-Reply-To: <1310399428.42173.YahooMailClassic@web65705.mail.ac4.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE0989F9AD@SBS2K8.premierlab.local> I would think that you could potentially use an SOP for frozen sections in the clinical setting, such as following bloodborne pathogens standards. I would ban the use of freeze spray in the cryostat and that you may want to use a particulate respirator such as the #M N95 1860S, and other standard PPE's that you use when sectioning frozen sections in the clinical setting. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 11, 2011 9:50 AM To: histonet@lists.utsouthwestern.edu; SaroBascaramurty Subject: Re: [Histonet] Written SOP Really? Do you think that anybody is going to have an SOP for such an specific and narrow subject? Please remember that an SOP procedure is nothing other than the description of the method you use in a way that anybody else can follow it, and do the same thing always. I think that you will be better of if you describe how do you complete such an assignment. Ren? K. --- On Mon, 7/11/11, Bascaramurty, Saro wrote: From: Bascaramurty, Saro Subject: [Histonet] Written SOP To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 11, 2011, 11:40 AM Hi, I am wondering if anyone of you have a written SOP on 'Cryosectioning of animal tissues implanted with human cells that are totally not screened for Hep B, HEP C, HIV, Mycobacterium tuberculosis and other adventitious agents' . If you have one written, would you be kind enough to share with me? Thanks in advance! Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramurty@nrc-cnrc.gc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Mon Jul 11 11:08:11 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Mon Jul 11 11:08:14 2011 Subject: [Histonet] Test Message-ID: So I got married and my name changed (meaning so did my email) tried sending out a broad email, but it didn't work. Just making sure I'm updated now =) Happy Monday all!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From thiggins <@t> cddmedical.com Mon Jul 11 11:28:02 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Mon Jul 11 11:28:11 2011 Subject: Fw: [Histonet] Re: Embedding Media Message-ID: <006701cc3fe7$813ee600$e001a8c0@cdd.loc> > Last comment on the paraffin conversion for me. > > You can use bees wax if you want in your processor and embedder that doesn't > mean you going to get the optimal results for every situation. Paraffin > types are a personal preference for most people or labs. It's a little more > hassle to keep two paraffins on hand instead of just one.. Low polymer > paraffins penetrate better, faster, so and so forth and the higher polymer > paraffins take a little longer but supports tissue better for sectioning. > There are positives and negatives for both. Using one paraffin type is > fine, all personal preference. We could go on forever with this!! > > > I am not endorsing any paraffin on here, used many different paraffins and > each work as anticipated. > > Thanks, > > Tim > > > > > Sent: Monday, July 11, 2011 5:44 AM > Subject: Re: [Histonet] Re: Embedding Media > > > Amita, > > The paraffin that we use is also a Leica product. It was formerly sold under > the Surgipath label, but since to companies have merged together, it now > sells under the Leica name. It is a "beaded" pellet paraffin and has a > version suitable for infiltration and a version suitable for embedding. The > box of the embedding paraffin suggests it can be utilized for *both* > purposes, however we do not choose to use it that way. So yes, we use two > different types of paraffin. > > Hope that helps! > Michelle > > > > I continuation of same discussion , i would like to have clarification for > > our lab..we use ....beaded paraffin "Leica Paraplast" both for processing > > and embedding . Do you advice us to use different paraffin for both steps? > > > > > > Paula, Please share if you get any feed back on Richard Allan product. > > > > Amita > > > > > > > Subject: [Histonet] Re: Embedding Media > > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > > > > > Hi Paula, > > > > Obviously there are no "compression free" paraffin's on the market. Look > > for a paraffin with a higher polymer content, this makes the paraffin > > firmer > > and less likely to compress. Other factor for into section compression > > but > > paraffin is a good place to start. > > > > The higher polymer paraffin's are not as suited for processing, it is more > > for the embedding portion of the process. > > > > Use a paraffin with lower polymers content in the processor and higher for > > embedding and you will like the results. > > > > > > Tim > > > > > > > Subject: [Histonet] Embedding Media > > To: > > > > > Hello, > > > > > > > > We are considering a switch to a different brand of paraffin and this is > > because I feel we are having too many compressions in some of our tissue > > sections. Currently, we use Tissue Path Paraplast, regular. > > > > > > > > I would like to get feedback from you as to what you prefer. Looking on > > line, Richard Allan has a product called Signature Series Paraffin that > > comes in a type L that offers compression-free sections and I was also > > hoping to get any feedback on that product. > > > > > > > > I would greatly appreciate any suggestions and thoughts. > > > > Thanks in advance, > > > > Paula > > > > > > > From lpjones <@t> srhs-pa.org Mon Jul 11 12:15:32 2011 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Mon Jul 11 12:17:32 2011 Subject: [Histonet] prostate bx containers In-Reply-To: References: , Message-ID: <4AE8039AEA096143B965CBC6D092166802351B3938@EXCH2007.srhs-pa.org> We looked at these, but our Pathologist's first question was "can we bill for 6 or 8 bottles if they are technically all in one?" How is that handled? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Monday, July 11, 2011 11:44 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We don't currently use them but I've used them in the past. As long as you don't 'bump' them on the grossing station while they're open, they're fine. I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ________________________________ From: Sheila Adey [mailto:sadey@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From histotech <@t> imagesbyhopper.com Mon Jul 11 12:24:18 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Jul 11 12:24:36 2011 Subject: [Histonet] Test In-Reply-To: References: Message-ID: <69D062BA-014C-4910-85FD-90CFD190C4DA@imagesbyhopper.com> CONGRATS!!! :-D Sent from my iPhone On Jul 11, 2011, at 12:08 PM, wrote: > So I got married and my name changed (meaning so did my email) tried > sending out a broad email, but it didn't work. Just making sure I'm > updated now =) > > Happy Monday all!! > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria <@t> gmail.com Mon Jul 11 12:34:16 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Jul 11 12:34:19 2011 Subject: [Histonet] prostate bx containers In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B3938@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D092166802351B3938@EXCH2007.srhs-pa.org> Message-ID: The sites are individual according to the specimen requisition form and on the container. It is in how the labeling is done. Also when the pathologist reads them out they will be reading by site/position. I've never used these containers but have received specimens in a single container individually wrapped and identified where the pathologist read them as they were. Vikki On Jul 11, 2011 1:17 PM, "Jones, Laura" wrote: > We looked at these, but our Pathologist's first question was "can we bill for 6 or 8 bottles if they are technically all in one?" How is that handled? > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [ mjdessoye@wvhcs.org] > Sent: Monday, July 11, 2011 11:44 AM > To: Sheila Adey; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] prostate bx containers > > We don't currently use them but I've used them in the past. As long as you don't 'bump' them on the grossing station while they're open, they're fine. I have yet to see one leak. > > Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | > 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 > > ________________________________ > > From: Sheila Adey [mailto:sadey@hotmail.ca] > Sent: Sat 7/9/2011 9:36 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prostate bx containers > > > > > Hello: > > Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. > We are going to trial them but I would like some opinions on them. > We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. > Thanks > Sheila > > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Wyoming Valley Health Care System. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sharon Regional Health System is the area's largest hospital > and provider of health care services. Visit us online at > http://www.sharonregional.com for a complete listing of our > services, primary care physicians and specialists, and satellite locations. > > Confidentiality Note: This message is intended for use > only by the individual or entity to which it is addressed > and may contain information that is privileged, > confidential, and exempt from disclosure under applicable > law. If the reader of this message is not the intended > recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. If > you have received this communication in error, please > contact the sender immediately and destroy the material in > its entirety, whether electronic or hard copy. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Mon Jul 11 13:03:12 2011 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Mon Jul 11 13:03:19 2011 Subject: [Histonet] Waste disposal Message-ID: <2B2ECF33934F5D4996D8BE03EFDF397608503ACC@VHAV09MSGA3.v09.med.va.gov> For any waste disposal you have to check with city, state and federal regulations for all of the rules and then usually you will follow the one with the highest regulation to cover yourself. I work at a VA and have the good fortune to have a whole department to utilize for all of our waste disposal questions. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From mwich <@t> 7thwavelabs.com Mon Jul 11 13:37:52 2011 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Mon Jul 11 13:37:57 2011 Subject: [Histonet] pan B-cell marker for frozen mouse tissue Message-ID: <62A8156F8071C8439080D626DF8C33A6018C0BF6@wave-mail.7thwave.local> I am trying to find the most appropriate pan B-cell marker for IHC on frozen mouse tissue. I know that CD45R/B220 used to be the most commonly used, but recent publications suggest PAX5 or CD19 as more restricted to the B-cell lineage. Does anyone have any other suggestions, or are these generally what people are using these days? Thanks for any info. From anonwums1 <@t> gmail.com Mon Jul 11 14:02:43 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Jul 11 14:02:48 2011 Subject: [Histonet] pan B-cell marker for frozen mouse tissue In-Reply-To: <62A8156F8071C8439080D626DF8C33A6018C0BF6@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A6018C0BF6@wave-mail.7thwave.local> Message-ID: B220 is a pretty good B-cell marker, although you're right that it's not restricted to the B-cell lineage. It's also expressed in some T-cells and NK cells, for example. Cells that are double positive for B220 and IgG or IgM are B-cells almost exclusively. CD19 is a good marker for mature B-cells, although pre-pro B cells do not express CD19. If you're looking at any organ other than bone marrow or spleen, there shouldn't be many pre-pro B cells and you can probably just use CD19. Adam On Mon, Jul 11, 2011 at 1:37 PM, Michele Wich wrote: > I am trying to find the most appropriate pan B-cell marker for IHC on > frozen mouse tissue. I know that CD45R/B220 used to be the most commonly > used, but recent publications suggest PAX5 or CD19 as more restricted to > the B-cell lineage. Does anyone have any other suggestions, or are these > generally what people are using these days? > > Thanks for any info. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shehnazster <@t> gmail.com Mon Jul 11 14:19:32 2011 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Mon Jul 11 14:19:35 2011 Subject: [Histonet] specimen container analytic interference Message-ID: Hi Netters, Please shed some light on the ffg: specimen container analytic interference. What should I do to comply with this standard? Thanx again S Kahn From rjbuesa <@t> yahoo.com Mon Jul 11 14:53:56 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 11 14:53:59 2011 Subject: [Histonet] prostate bx containers In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B3938@EXCH2007.srhs-pa.org> Message-ID: <1310414036.456.YahooMailClassic@web65709.mail.ac4.yahoo.com> Your pathologist is right. You cannot. Billing is PER block Ren? J. --- On Mon, 7/11/11, Jones, Laura wrote: From: Jones, Laura Subject: RE: [Histonet] prostate bx containers To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 11, 2011, 1:15 PM We looked at these, but our Pathologist's first question was "can we bill for 6 or 8 bottles if they are technically all in one?"? How is that handled? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Monday, July 11, 2011 11:44 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We don't currently use them but I've used them in the past.? As long as you don't 'bump' them on the grossing station while they're open, they're fine.? I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ________________________________ From: Sheila Adey [mailto:sadey@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note:? This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited.? If you have received this communication in error,? please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy.? Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nacaela.Johnson <@t> USONCOLOGY.COM Mon Jul 11 15:02:46 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Mon Jul 11 15:02:49 2011 Subject: [Histonet] prostate bx containers In-Reply-To: <1310414036.456.YahooMailClassic@web65709.mail.ac4.yahoo.com> References: <4AE8039AEA096143B965CBC6D092166802351B3938@EXCH2007.srhs-pa.org> <1310414036.456.YahooMailClassic@web65709.mail.ac4.yahoo.com> Message-ID: <71882EED22A283429E8424513A22922D595283@txhous1eb015.uson.usoncology.int> Isn't it better to generate more blocks when so many cores are put in one vial? Therefore, the billing would be for the amount of blocks created correct? Thanks, Nacaela -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 11, 2011 2:54 PM To: histonet@lists.utsouthwestern.edu; LauraJones Subject: RE: [Histonet] prostate bx containers Your pathologist is right. You cannot. Billing is PER block Ren? J. --- On Mon, 7/11/11, Jones, Laura wrote: From: Jones, Laura Subject: RE: [Histonet] prostate bx containers To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 11, 2011, 1:15 PM We looked at these, but our Pathologist's first question was "can we bill for 6 or 8 bottles if they are technically all in one?"? How is that handled? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Monday, July 11, 2011 11:44 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We don't currently use them but I've used them in the past.? As long as you don't 'bump' them on the grossing station while they're open, they're fine.? I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ________________________________ From: Sheila Adey [mailto:sadey@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note:? This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited.? If you have received this communication in error,? please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy.? Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From HornHV <@t> archildrens.org Mon Jul 11 15:21:25 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jul 11 15:21:28 2011 Subject: [Histonet] prostate bx containers In-Reply-To: <1310414036.456.YahooMailClassic@web65709.mail.ac4.yahoo.com> References: <4AE8039AEA096143B965CBC6D092166802351B3938@EXCH2007.srhs-pa.org> <1310414036.456.YahooMailClassic@web65709.mail.ac4.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB7198B85ACBA@EVS1.archildrens.org> According to the CPT code book it is per specimen. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 11, 2011 2:54 PM To: histonet@lists.utsouthwestern.edu; LauraJones Subject: RE: [Histonet] prostate bx containers Your pathologist is right. You cannot. Billing is PER block Ren? J. --- On Mon, 7/11/11, Jones, Laura wrote: From: Jones, Laura Subject: RE: [Histonet] prostate bx containers To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 11, 2011, 1:15 PM We looked at these, but our Pathologist's first question was "can we bill for 6 or 8 bottles if they are technically all in one?"? How is that handled? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Monday, July 11, 2011 11:44 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We don't currently use them but I've used them in the past.? As long as you don't 'bump' them on the grossing station while they're open, they're fine.? I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ________________________________ From: Sheila Adey [mailto:sadey@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note:? This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited.? If you have received this communication in error,? please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy.? Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From trathborne <@t> somerset-healthcare.com Mon Jul 11 15:21:08 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Jul 11 15:22:05 2011 Subject: [Histonet] prostate bx containers In-Reply-To: <1310414036.456.YahooMailClassic@web65709.mail.ac4.yahoo.com> References: <4AE8039AEA096143B965CBC6D092166802351B3938@EXCH2007.srhs-pa.org> <1310414036.456.YahooMailClassic@web65709.mail.ac4.yahoo.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570702FD44@SMCMAIL01.somerset-healthcare.com> Billing, I believe is per specimen, not per block. You may receive a specimen and divide it into 20 blocks, but it is still just one charge. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 11, 2011 3:54 PM To: histonet@lists.utsouthwestern.edu; LauraJones Subject: RE: [Histonet] prostate bx containers Your pathologist is right. You cannot. Billing is PER block Ren? J. --- On Mon, 7/11/11, Jones, Laura wrote: From: Jones, Laura Subject: RE: [Histonet] prostate bx containers To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 11, 2011, 1:15 PM We looked at these, but our Pathologist's first question was "can we bill for 6 or 8 bottles if they are technically all in one?"? How is that handled? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Monday, July 11, 2011 11:44 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We don't currently use them but I've used them in the past.? As long as you don't 'bump' them on the grossing station while they're open, they're fine.? I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ________________________________ From: Sheila Adey [mailto:sadey@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note:? This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited.? If you have received this communication in error,? please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy.? Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From emilystebens <@t> gmail.com Mon Jul 11 17:51:15 2011 From: emilystebens <@t> gmail.com (emily stebens) Date: Mon Jul 11 17:51:20 2011 Subject: [Histonet] Re:Waste disposal Message-ID: > We used Ventana Ultra in our lab. We sent a sample of the waste to our > safety department and they had the sample tested. They told us that it > could be dumped down the sink. From sabeti_shahram <@t> yahoo.com Mon Jul 11 22:24:43 2011 From: sabeti_shahram <@t> yahoo.com (Shahram Sabeti) Date: Mon Jul 11 22:24:47 2011 Subject: [Histonet] restaining method of wright-giemsa stained peripheral blood slides Message-ID: <1310441083.76362.YahooMailNeo@web35606.mail.mud.yahoo.com> dear fellows, ??? I have some?precious blood slides at hand but with a major problem,pallor, because all of them are unmounted and a long time has elapsed from their preparation .their restaining was frightening for me because a fault could result in? their deterioration. ?? do you have any experience in this respect.thank you?in advance for your help. sincerely yours, sabeti? From rjbuesa <@t> yahoo.com Tue Jul 12 08:57:55 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 12 08:57:59 2011 Subject: [Histonet] restaining method of wright-giemsa stained peripheral blood slides In-Reply-To: <1310441083.76362.YahooMailNeo@web35606.mail.mud.yahoo.com> Message-ID: <1310479075.99866.YahooMailClassic@web65715.mail.ac4.yahoo.com> Prepare a 1% alcoholic (100 EthOL) solution of acetic acid and immerse (one by one) the smears in it untilll all the color leaches out. Wash them thoroughly in distilled water, and restain them as usual with your Wright-Giemsa solution.Ren? J. --- On Mon, 7/11/11, Shahram Sabeti wrote: From: Shahram Sabeti Subject: [Histonet] restaining method of wright-giemsa stained peripheral blood slides To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 11, 2011, 11:24 PM dear fellows, ??? I have some?precious blood slides at hand but with a major problem,pallor, because all of them are unmounted and a long time has elapsed from their preparation .their restaining was frightening for me because a fault could result in? their deterioration. ?? do you have any experience in this respect.thank you?in advance for your help. sincerely yours, sabeti? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nele.Degryse <@t> UGent.be Tue Jul 12 09:07:10 2011 From: Nele.Degryse <@t> UGent.be (Nele Degryse) Date: Tue Jul 12 09:07:16 2011 Subject: [Histonet] Remove me Message-ID: <20110712160710.833429umteozzx1q@webmail.ugent.be> Could you please remove me from the mailing list? Kind regards, Nele Degryse From Julia.Koob <@t> nygh.on.ca Tue Jul 12 09:16:39 2011 From: Julia.Koob <@t> nygh.on.ca (Julia Koob) Date: Tue Jul 12 09:17:22 2011 Subject: [Histonet] Remove me In-Reply-To: <20110712160710.833429umteozzx1q@webmail.ugent.be> References: <20110712160710.833429umteozzx1q@webmail.ugent.be> Message-ID: <7E804D2A4A37154E8CB0C5E0A8D73B39099A725AFF@NYGH-EXCHSERV.nygh.local> Yes, please remove me fromt he mailing list as well.. Julia Koob Technical Co-ordinator, Histology Laboratory North York General Hospital 416-756-6892 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nele Degryse [Nele.Degryse@UGent.be] Sent: Tuesday, July 12, 2011 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remove me Could you please remove me from the mailing list? Kind regards, Nele Degryse _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- This email message and any attachments are intended only for the use of the individual to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the recipient of this email is not the intended recipient (or the employee or agent responsible for delivering the email to the intended recipient), you are hereby notified that any review, dissemination, distribution or copying or other use of this message is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return email and delete this message and attachments from your system. Thank You. ----- From sbaldwin <@t> mhhcc.org Tue Jul 12 10:36:42 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Jul 12 10:36:43 2011 Subject: [Histonet] WASTE DISPOSAL Message-ID: Histonetters The stericycle chemists came to our place and looked at all our MSDS sheets that go with the Ventana by products, according to him it is not hazardous waste but most of the byproduct is liquid coverslip which is very oily, therefor he suggest we haul it off with the hazardous waste and this is what we are doing. Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From mward <@t> wfubmc.edu Tue Jul 12 12:25:37 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Jul 12 12:25:50 2011 Subject: [Histonet] Langerin antibody Message-ID: Hi all! I have been asked by one of our Pathology fellows to see if anyone is performing this antibody-Langerin- and if so, would you be willing to perform it for us. It is not something that we would be adding to our test menu at this time. Please let me know the fee and how many slides you would need. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From ryramirez <@t> cox.net Tue Jul 12 12:37:54 2011 From: ryramirez <@t> cox.net (=?utf-8?B?cnlyYW1pcmV6QGNveC5uZXQ=?=) Date: Tue Jul 12 12:37:46 2011 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUmVtb3ZlIG1l?= Message-ID: <20110712173740.MNIY3912.fed1rmfepo102.cox.net@fed1rmimpo02.cox.net> Remove me too. Sent from my Verizon Wireless Phone ----- Reply message ----- From: "Julia Koob" Date: Tue, Jul 12, 2011 7:16 am Subject: [Histonet] Remove me To: "Nele Degryse" , "histonet@lists.utsouthwestern.edu" Yes, please remove me fromt he mailing list as well.. Julia Koob Technical Co-ordinator, Histology Laboratory North York General Hospital 416-756-6892 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nele Degryse [Nele.Degryse@UGent.be] Sent: Tuesday, July 12, 2011 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remove me Could you please remove me from the mailing list? Kind regards, Nele Degryse _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- This email message and any attachments are intended only for the use of the individual to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the recipient of this email is not the intended recipient (or the employee or agent responsible for delivering the email to the intended recipient), you are hereby notified that any review, dissemination, distribution or copying or other use of this message is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return email and delete this message and attachments from your system. Thank You. ----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Jul 12 12:40:39 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Jul 12 12:41:28 2011 Subject: [Histonet] Remove me In-Reply-To: <20110712173740.MNIY3912.fed1rmfepo102.cox.net@fed1rmimpo02.cox.net> References: <20110712173740.MNIY3912.fed1rmfepo102.cox.net@fed1rmimpo02.cox.net> Message-ID: you have to remove yourself. follow the instructions. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ryramirez@cox.net [ryramirez@cox.net] Sent: Tuesday, July 12, 2011 1:37 PM To: Julia Koob; Nele Degryse; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Remove me Remove me too. Sent from my Verizon Wireless Phone ----- Reply message ----- From: "Julia Koob" Date: Tue, Jul 12, 2011 7:16 am Subject: [Histonet] Remove me To: "Nele Degryse" , "histonet@lists.utsouthwestern.edu" Yes, please remove me fromt he mailing list as well.. Julia Koob Technical Co-ordinator, Histology Laboratory North York General Hospital 416-756-6892 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nele Degryse [Nele.Degryse@UGent.be] Sent: Tuesday, July 12, 2011 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remove me Could you please remove me from the mailing list? Kind regards, Nele Degryse _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- This email message and any attachments are intended only for the use of the individual to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the recipient of this email is not the intended recipient (or the employee or agent responsible for delivering the email to the intended recipient), you are hereby notified that any review, dissemination, distribution or copying or other use of this message is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return email and delete this message and attachments from your system. Thank You. ----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lscott <@t> sfcn.org Tue Jul 12 13:21:38 2011 From: lscott <@t> sfcn.org (lscott@sfcn.org) Date: Tue Jul 12 13:21:43 2011 Subject: [Histonet] What is a great manual mirotome Message-ID: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) From trathborne <@t> somerset-healthcare.com Tue Jul 12 13:29:56 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jul 12 13:31:36 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570703008B@SMCMAIL01.somerset-healthcare.com> We have the 2235 and some 2135's, and have been satisfied with all of them. Just the usual wear and tear problems seen over time. Do you have Leica service your current microtome? Is it always the same problem? Most service companies will offer a 30 day guarantee on their work. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lscott@sfcn.org Sent: Tuesday, July 12, 2011 2:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From liz <@t> premierlab.com Tue Jul 12 13:35:41 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jul 12 13:35:49 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> Message-ID: <14E2C6176416974295479C64A11CB9AE0989F9C4@SBS2K8.premierlab.local> I find it hard to believe that you are not getting what you need from the Leica 2235, I don't have that particular model we have two RM2255 and one RM2145 and we just love them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lscott@sfcn.org Sent: Tuesday, July 12, 2011 12:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nacaela.Johnson <@t> USONCOLOGY.COM Tue Jul 12 13:45:58 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Tue Jul 12 13:46:06 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <14E2C6176416974295479C64A11CB9AE0989F9C4@SBS2K8.premierlab.local> References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> <14E2C6176416974295479C64A11CB9AE0989F9C4@SBS2K8.premierlab.local> Message-ID: <71882EED22A283429E8424513A22922D595291@txhous1eb015.uson.usoncology.int> I use the RM2235 and absolutely love it. I have only had wear and tear issues as well. What kind of inconsistency are you having with yours? Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, July 12, 2011 1:36 PM To: 'lscott@sfcn.org'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What is a great manual mirotome I find it hard to believe that you are not getting what you need from the Leica 2235, I don't have that particular model we have two RM2255 and one RM2145 and we just love them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lscott@sfcn.org Sent: Tuesday, July 12, 2011 12:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From Nikki.Wahlberg <@t> bsci.com Tue Jul 12 13:48:13 2011 From: Nikki.Wahlberg <@t> bsci.com (Wahlberg, Nikki) Date: Tue Jul 12 13:48:22 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <14E2C6176416974295479C64A11CB9AE0989F9C4@SBS2K8.premierlab.local> References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> <14E2C6176416974295479C64A11CB9AE0989F9C4@SBS2K8.premierlab.local> Message-ID: <87224B7198E1794A8C5E679FF761CCC70EA14C4DD2@MAPEXCMSP01.bsci.bossci.com> We use the Leica RM2255, RM2235, RM2165 and RM2265 and we love all of them! We are a research lab and are cutting paraffin and plastic. What are the specific issues that you are having with them? Nikki Nikki M Wahlberg Histology Lab Supervisor Preclinical Sciences Boston Scientific 763-694-5739 wahlbern@bsci.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, July 12, 2011 1:36 PM To: 'lscott@sfcn.org'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What is a great manual mirotome I find it hard to believe that you are not getting what you need from the Leica 2235, I don't have that particular model we have two RM2255 and one RM2145 and we just love them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lscott@sfcn.org Sent: Tuesday, July 12, 2011 12:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Tue Jul 12 14:11:51 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Tue Jul 12 14:11:58 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> Message-ID: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lscott@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Tue Jul 12 14:25:46 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Jul 12 14:25:58 2011 Subject: [Histonet] What is a great manual microtomes In-Reply-To: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> Message-ID: We have two Leica's and love them a RM 2255 and a RM2235. No problems. A real work horse! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net lscott@sfcn.org Sent by: histonet-bounces@lists.utsouthwestern.edu 07/12/2011 02:22 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From sbreeden <@t> nmda.nmsu.edu Tue Jul 12 14:32:46 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jul 12 14:32:51 2011 Subject: [Histonet] What is a great manual microtomes In-Reply-To: References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF86E@nmdamailsvr.nmda.ad.nmsu.edu> What she said! RM2235 without question! From foreightl <@t> gmail.com Tue Jul 12 14:40:12 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Jul 12 14:40:20 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> Message-ID: I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, wrote: > I am a manual tome junky! I like the Thermo Finesse (I think it's 325). > These are the old microms...they work like a charm =) > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > lscott@sfcn.org > Sent: Tuesday, July 12, 2011 1:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] What is a great manual mirotome > > Hi, > Our small lab is looking for some advise on what microtome to replace a > fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 > and the Leica 2125. We are looking for reliability and have net gotten > it > from the RM2235. Is anyone using either of these that would be willing > to > offer suggestions? > > Thanks, > > Scott Hendricksen HT (ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From b-frederick <@t> northwestern.edu Tue Jul 12 14:46:28 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jul 12 14:46:37 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E11AFFF@evcspmbx2.ads.northwestern.edu> I want my AO820 back! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdysart@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, wrote: > I am a manual tome junky! I like the Thermo Finesse (I think it's 325). > These are the old microms...they work like a charm =) > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > lscott@sfcn.org > Sent: Tuesday, July 12, 2011 1:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] What is a great manual mirotome > > Hi, > Our small lab is looking for some advise on what microtome to replace > a fairly new Leica RM2235 with. We have been looking at the Sakura SRM > 200 and the Leica 2125. We are looking for reliability and have net > gotten it from the RM2235. Is anyone using either of these that would > be willing to offer suggestions? > > Thanks, > > Scott Hendricksen HT (ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jsjurczak <@t> comcast.net Tue Jul 12 14:47:27 2011 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Tue Jul 12 14:47:37 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: Message-ID: <493187241.500642.1310500047053.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> We have tried the Leicas thru the years and found that post 2030's that our arms were not long enough to turn the handwheel without completely straightening our elbows. This led to soreness in our elbows. Their microtomes kept getting larger and larger with the handwheel further back. The 2125 solves that problem for us. We really like ours. ----- Original Message ----- From: "Patrick Laurie" To: sdysart@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Sent: Tuesday, July 12, 2011 2:40:12 PM Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, wrote: > I am a manual tome junky! I like the Thermo Finesse (I think it's 325). > These are the old microms...they work like a charm =) > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > lscott@sfcn.org > Sent: Tuesday, July 12, 2011 1:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] What is a great manual mirotome > > Hi, > Our small lab is looking for some advise on what microtome to replace a > fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 > and the Leica 2125. We are looking for reliability and have net gotten > it > from the RM2235. Is anyone using either of these that would be willing > to > offer suggestions? > > Thanks, > > Scott Hendricksen HT (ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve8438 <@t> gmail.com Tue Jul 12 14:49:18 2011 From: steve8438 <@t> gmail.com (Steve Mello) Date: Tue Jul 12 14:49:30 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <493187241.500642.1310500047053.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> References: <493187241.500642.1310500047053.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Message-ID: <1794423046-1310500159-cardhu_decombobulator_blackberry.rim.net-1995947896-@b3.c1.bise6.blackberry> Leitz 1512. Great workhorse if u can find one Sent via BlackBerry by AT&T -----Original Message----- From: jsjurczak@comcast.net Sender: histonet-bounces@lists.utsouthwestern.edu Date: Tue, 12 Jul 2011 19:47:27 To: Patrick Laurie Cc: Subject: Re: [Histonet] What is a great manual mirotome We have tried the Leicas thru the years and found that post 2030's that our arms were not long enough to turn the handwheel without completely straightening our elbows. This led to soreness in our elbows. Their microtomes kept getting larger and larger with the handwheel further back. The 2125 solves that problem for us. We really like ours. ----- Original Message ----- From: "Patrick Laurie" To: sdysart@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Sent: Tuesday, July 12, 2011 2:40:12 PM Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, wrote: > I am a manual tome junky! I like the Thermo Finesse (I think it's 325). > These are the old microms...they work like a charm =) > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > lscott@sfcn.org > Sent: Tuesday, July 12, 2011 1:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] What is a great manual mirotome > > Hi, > Our small lab is looking for some advise on what microtome to replace a > fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 > and the Leica 2125. We are looking for reliability and have net gotten > it > from the RM2235. Is anyone using either of these that would be willing > to > offer suggestions? > > Thanks, > > Scott Hendricksen HT (ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Jul 12 14:51:41 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jul 12 14:51:46 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <493187241.500642.1310500047053.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> References: <493187241.500642.1310500047053.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E11B01B@evcspmbx2.ads.northwestern.edu> I actually use a Microm HM315 and have never had issues with it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jsjurczak@comcast.net Sent: Tuesday, July 12, 2011 2:47 PM To: Patrick Laurie Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome We have tried the Leicas thru the years and found that post 2030's that our arms were not long enough to turn the handwheel without completely straightening our elbows. This led to soreness in our elbows. Their microtomes kept getting larger and larger with the handwheel further back. The 2125 solves that problem for us. We really like ours. ----- Original Message ----- From: "Patrick Laurie" To: sdysart@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Sent: Tuesday, July 12, 2011 2:40:12 PM Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, wrote: > I am a manual tome junky! I like the Thermo Finesse (I think it's 325). > These are the old microms...they work like a charm =) > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > lscott@sfcn.org > Sent: Tuesday, July 12, 2011 1:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] What is a great manual mirotome > > Hi, > Our small lab is looking for some advise on what microtome to replace > a fairly new Leica RM2235 with. We have been looking at the Sakura SRM > 200 and the Leica 2125. We are looking for reliability and have net > gotten it from the RM2235. Is anyone using either of these that would > be willing to offer suggestions? > > Thanks, > > Scott Hendricksen HT (ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Jul 12 14:55:46 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Jul 12 14:55:54 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E11AFFF@evcspmbx2.ads.northwestern.edu> References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> <62C639732D3F274DACED033EBDF6ADAF1E11AFFF@evcspmbx2.ads.northwestern.edu> Message-ID: <1310500546.96759.YahooMailRC@web1109.biz.mail.sk1.yahoo.com> I still use AO820s!!! Keep them greased and oiled and they will just keep going, and going, and going.... ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Bernice Frederick To: Patrick Laurie ; "sdysart@mirnarx.com" Cc: "Histonet@lists.utsouthwestern.edu" Sent: Tue, July 12, 2011 2:46:28 PM Subject: RE: [Histonet] What is a great manual mirotome I want my AO820 back! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdysart@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265.? A good non-automated version is the RM 2235.? I've also used the Microm (thermo-fisher-shandon) HM325S which work well.? The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025.? I however have never used the Leica 2125.? I've always had good service from them, especially if you have a service contract.? Good luck! On Tue, Jul 12, 2011 at 12:11 PM, wrote: > I am a manual tome junky!? I like the Thermo Finesse (I think it's 325). > These are the old microms...they work like a charm =) > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas? 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > lscott@sfcn.org > Sent: Tuesday, July 12, 2011 1:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] What is a great manual mirotome > >? Hi, > Our small lab is looking for some advise on what microtome to replace > a fairly new Leica RM2235 with. We have been looking at the Sakura SRM > 200 and the Leica 2125. We are looking for reliability and have net > gotten it from the RM2235. Is anyone using either of these that would > be willing to offer suggestions? > > Thanks, > > Scott Hendricksen HT (ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Jul 12 14:57:28 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Jul 12 14:58:29 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E11AFFF@evcspmbx2.ads.northwestern.edu> References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> <62C639732D3F274DACED033EBDF6ADAF1E11AFFF@evcspmbx2.ads.northwestern.edu> Message-ID: <4E1CA728.9020000@pathology.washington.edu> The old black ones, gotta love them. Most of the young techs out there probably have never seen one. Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 7/12/2011 12:46 PM, Bernice Frederick wrote: > I want my AO820 back! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie > Sent: Tuesday, July 12, 2011 2:40 PM > To: sdysart@mirnarx.com > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] What is a great manual mirotome > > I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! > > On Tue, Jul 12, 2011 at 12:11 PM, wrote: > >> I am a manual tome junky! I like the Thermo Finesse (I think it's 325). >> These are the old microms...they work like a charm =) >> >> Sarah Goebel-Dysart, BA, HT(ASCP) >> Histotechnologist >> Mirna Therapeutics >> 2150 Woodward Street >> Suite 100 >> Austin, Texas 78744 >> (512)901-0900 ext. 6912 >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> lscott@sfcn.org >> Sent: Tuesday, July 12, 2011 1:22 PM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] What is a great manual mirotome >> >> Hi, >> Our small lab is looking for some advise on what microtome to replace >> a fairly new Leica RM2235 with. We have been looking at the Sakura SRM >> 200 and the Leica 2125. We are looking for reliability and have net >> gotten it from the RM2235. Is anyone using either of these that would >> be willing to offer suggestions? >> >> Thanks, >> >> Scott Hendricksen HT (ASCP) >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology& Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > plaurie@cellnetix.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From techonebs <@t> comcast.net Tue Jul 12 17:46:02 2011 From: techonebs <@t> comcast.net (Matt Mincer) Date: Tue Jul 12 17:44:09 2011 Subject: [Histonet] Histobath Message-ID: <4E1CCEAA.7000506@comcast.net> Hey All, I am working with a company to develop a replacement for the Histobath. They should have a prototype to me by the end of the week. I am looking for a lab, preferably in the Chicago, that is willing to test and assess the product. Let me know if you are interested. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 From ltougas <@t> dawsoncollege.qc.ca Tue Jul 12 18:15:53 2011 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Tue Jul 12 18:16:03 2011 Subject: [Histonet] microtome knife holder In-Reply-To: References: <7618BC6D53F39149B7B57615C218AADF275F8672D9@EXCHANGE.ad.dawsoncollege.qc.ca>, <1310133833.32667.YahooMailClassic@web65709.mail.ac4.yahoo.com>, Message-ID: <7618BC6D53F39149B7B57615C218AADF275F8672E9@EXCHANGE.ad.dawsoncollege.qc.ca> Thank you Hugh and all others who replied. In fact what I really meant was rather a knife "clamp", I guess, as on the older microtomes, where we insert either a regular knife. So my next question is: does anyone still have a knife "clamp" for either a 2030 Reichert Jung microtome or a Leica microtome. I found those pictures on the web that represent what I am looking for. Thank you again in advance, Liette Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal, Qc 514-931-8731, ext 1519 [http://images.dotmed.com/cgi-bin/size.pl?t=2&a=3&i=779208.jpg][http://websites.labx.com/rankin/pics/3066.JPG] ________________________________ From: Hugh Luk [hlukey@msn.com] Sent: July 12, 2011 4:04 PM To: Liette Tougas Subject: RE: [Histonet] microtome knife holder Liette, The Reichert Jung 2030 came with a choice of disposable blade (see picture) or fixed blade (see link) holders. One...never both:-( http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=110710454143&clk_rvr_id=247196707126 When Leica purchased Reichert, Jung and other microtome lines, they absorbed them into their new units. Meaning, they do not sell parts or encourage repair of older units. We have replaced all of our aging 2030's with 2255's. And sorry, they were disposed of. Rene is correct in his statement, sort of. Technically, there is a blade holder that looks like a long knife for disposable blades; if you had this and the fixed blade holder, you would be fine. I'm guessing Rene's lab used this set-up. Everyone in Hawaii, went the other way. Good luck with parts. Feel free to ask histonet again. Your best bet is to find someone who is getting rid of their unit, as the knife holder should have never worn out. Hugh Hawaii > Date: Fri, 8 Jul 2011 07:03:53 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; ltougas@dawsoncollege.qc.ca > Subject: Re: [Histonet] microtome knife holder > CC: > > Knives (not disposable blades) do not need holders. They are used by themselves. > Ren? J. > > --- On Thu, 7/7/11, Liette Tougas wrote: > > > From: Liette Tougas > Subject: [Histonet] microtome knife holder > To: "histonet@lists.utsouthwestern.edu" > Date: Thursday, July 7, 2011, 4:23 PM > > > Hi again everyone, > > I also wanted to ask if anyone has, or new if there was ever, a regular knife (not blade) holder for the Reichert Yung 2030 microtome and/or the Leica microtome series. > > thank you again in advance, > > Liette Tougas, RT, B.Sc., M.Sc. > Biomedical Laboratory Technology Department > Dawson College > 514-931-8731, ext 1519 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Tue Jul 12 19:04:51 2011 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Jul 12 19:04:59 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <4E1CA728.9020000@pathology.washington.edu> References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> , <62C639732D3F274DACED033EBDF6ADAF1E11AFFF@evcspmbx2.ads.northwestern.edu>, <4E1CA728.9020000@pathology.washington.edu> Message-ID: I paid $30.00 for my pre-owned Spencer 820. Liked it so well I bought two. It has served as a virtually foolproof and indestructible workhorse for 22 years. I have produced, literally more than a tone of slides with it. Jerry L Ricks Research Scientist University of Washington Department of Pathology > Date: Tue, 12 Jul 2011 12:57:28 -0700 > From: victor@pathology.washington.edu > To: b-frederick@northwestern.edu > Subject: Re: [Histonet] What is a great manual mirotome > CC: Histonet@lists.utsouthwestern.edu > > The old black ones, gotta love them. Most of the young techs out there > probably have never seen one. > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > On 7/12/2011 12:46 PM, Bernice Frederick wrote: > > I want my AO820 back! > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie > > Sent: Tuesday, July 12, 2011 2:40 PM > > To: sdysart@mirnarx.com > > Cc: Histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] What is a great manual mirotome > > > > I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! > > > > On Tue, Jul 12, 2011 at 12:11 PM, wrote: > > > >> I am a manual tome junky! I like the Thermo Finesse (I think it's 325). > >> These are the old microms...they work like a charm =) > >> > >> Sarah Goebel-Dysart, BA, HT(ASCP) > >> Histotechnologist > >> Mirna Therapeutics > >> 2150 Woodward Street > >> Suite 100 > >> Austin, Texas 78744 > >> (512)901-0900 ext. 6912 > >> > >> > >> -----Original Message----- > >> From: histonet-bounces@lists.utsouthwestern.edu > >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >> lscott@sfcn.org > >> Sent: Tuesday, July 12, 2011 1:22 PM > >> To: Histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] What is a great manual mirotome > >> > >> Hi, > >> Our small lab is looking for some advise on what microtome to replace > >> a fairly new Leica RM2235 with. We have been looking at the Sakura SRM > >> 200 and the Leica 2125. We are looking for reliability and have net > >> gotten it from the RM2235. Is anyone using either of these that would > >> be willing to offer suggestions? > >> > >> Thanks, > >> > >> Scott Hendricksen HT (ASCP) > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > -- > > Patrick Laurie HT(ASCP)QIHC > > CellNetix Pathology& Laboratories > > 1124 Columbia Street, Suite 200 > > Seattle, WA 98104 > > plaurie@cellnetix.com > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From techonebs <@t> comcast.net Tue Jul 12 19:16:59 2011 From: techonebs <@t> comcast.net (Matt Mincer) Date: Tue Jul 12 19:15:08 2011 Subject: [Histonet] Histobath revised Message-ID: <4E1CE3FB.4050702@comcast.net> Hey again. I was not refering to a tissue flotation bath. The Histobath was a product sold by Shandon. It had a chamber filled with isopentane or alcohol and was used for quick freezing tissue for a cryostat. Sorry if there was any confusion. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 From jshea121 <@t> roadrunner.com Tue Jul 12 20:40:07 2011 From: jshea121 <@t> roadrunner.com (Shea's) Date: Tue Jul 12 20:40:06 2011 Subject: [Histonet] What is a great manual mirotome Message-ID: Our 23 year old Reichert Jung 2030s, with high profile blades. They are work horses ....Only one repair (the spring). Maintenance contract on a microtome? From Diane.Tokugawa <@t> kp.org Wed Jul 13 00:33:25 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Wed Jul 13 00:33:44 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 07/12/2011 and will not return until 07/14/2011. Note: For Cytology issues, please call Molly at 8-421-5487, For Histology issues, please call the general histology lab 8-421- 5408. From tgenade <@t> gmail.com Wed Jul 13 06:23:11 2011 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Wed Jul 13 06:23:16 2011 Subject: [Histonet] confocal questions: mixing fluorescent dyes with proteins Message-ID: Hello, I'm working on a Zeiss confocal microscope. We use chiefly Alexa488 and Cy3 together with Hoechst 33258. Can anyone suggest a fluorescent protein which can be discerned amongst the Alexa488, Cy3 and Hoechst 33258 emission spectra? I was thinking maybe mBanana? Thanks -- Tyrone Genade email: tgenade@gmail.com tel: +27-84-632-1925 (c) Department of Human Biology University of Cape Town South Africa From sfonner <@t> labpath.com Wed Jul 13 06:39:00 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Wed Jul 13 06:42:58 2011 Subject: [Histonet] What is a great manual mirotome In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E11AFFF@evcspmbx2.ads.northwestern.edu> References: <15b0d0334296d5fa0be3d12c62d6ab76.squirrel@mail.sfcn.org> <62C639732D3F274DACED033EBDF6ADAF1E11AFFF@evcspmbx2.ads.northwestern.edu> Message-ID: <000001cc4151$75ce42f0$616ac8d0$@com> You sure can't beat an old AO! The old Leitz microtomes with the wheel on the front were awesome too. Ahhh memories. Sheila HT, ASCP KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Tuesday, July 12, 2011 3:46 PM To: Patrick Laurie; sdysart@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What is a great manual mirotome I want my AO820 back! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdysart@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, wrote: > I am a manual tome junky! I like the Thermo Finesse (I think it's 325). > These are the old microms...they work like a charm =) > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > lscott@sfcn.org > Sent: Tuesday, July 12, 2011 1:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] What is a great manual mirotome > > Hi, > Our small lab is looking for some advise on what microtome to replace > a fairly new Leica RM2235 with. We have been looking at the Sakura SRM > 200 and the Leica 2125. We are looking for reliability and have net > gotten it from the RM2235. Is anyone using either of these that would > be willing to offer suggestions? > > Thanks, > > Scott Hendricksen HT (ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Wed Jul 13 06:55:20 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Jul 13 06:55:31 2011 Subject: [Histonet] MT-5000 specimen holder Message-ID: <24A4826E8EF0964D86BC5317306F58A55DF6BE9079@mmc-mail.ad.mhsil.com> Looking for a MT5000 specimen holder. Old ultramicotrome we use for thick sectioning. Any ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From ragamouni.sravanthi <@t> gmail.com Wed Jul 13 07:35:29 2011 From: ragamouni.sravanthi <@t> gmail.com (ragamouni sravanthi) Date: Wed Jul 13 07:35:33 2011 Subject: [Histonet] Regarding Antigen retrieval technique for antigen collagen/osteoclacin Message-ID: hello, i am working on MMA embedded bone sections and i want to IHC of collagen so can anyone suggest me the the appropriate antigen retrieval technique to be used. Any suggestion would be encouraged.please help me out in this regard. With regards R.Sravanthi From mjdessoye <@t> wvhcs.org Wed Jul 13 07:50:08 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Wed Jul 13 07:52:11 2011 Subject: [Histonet] prostate bx containers References: , <4AE8039AEA096143B965CBC6D092166802351B3938@EXCH2007.srhs-pa.org> Message-ID: We still treat the specimens as though they were different specimen parts (A, B, C, D, etc...) I doubt that the physical arrangement of the containers affects the billing if you are still treating each core as a different specimen... ________________________________ From: Jones, Laura [mailto:lpjones@srhs-pa.org] Sent: Mon 7/11/2011 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We looked at these, but our Pathologist's first question was "can we bill for 6 or 8 bottles if they are technically all in one?" How is that handled? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Monday, July 11, 2011 11:44 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We don't currently use them but I've used them in the past. As long as you don't 'bump' them on the grossing station while they're open, they're fine. I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ________________________________ From: Sheila Adey [mailto:sadey@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From TroyerDA <@t> EVMS.EDU Wed Jul 13 08:04:12 2011 From: TroyerDA <@t> EVMS.EDU (Troyer, Dean A.) Date: Wed Jul 13 08:04:23 2011 Subject: [Histonet] prostate bx containers References: , <4AE8039AEA096143B965CBC6D092166802351B3938@EXCH2007.srhs-pa.org> Message-ID: <71EE6DB05CB563458E8CBA495B3E4BD003F563A2@romulus.evms.net> It might take a Phaldelphia lawyer to figure the multiwell Simport thing out. However, I am aware that the American Urological Association and conversations with other pathology leaders advocates treating one container as one CPT 88305. If you received six cores in one specimen "container" , don't assume you can separate those into six blocks and bill six 88305s. If you get six containers with one core in each container, you can bill six 88305s. Same amount of tissue. Same amount of work. Alice in Wonderland, but I wouldn't assume you can safely bill separately for tissue you've separated out of a biopsy container. Expecially core needle biopsies of the prostate. One of the reasons an 88305 technical fee was originally set rather high was to accomodate those nasty TURPs that would have a dozen blocks coming out of one container. Dean Troyer ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dessoye, Michael J Sent: Wed 7/13/2011 8:50 AM To: Jones, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We still treat the specimens as though they were different specimen parts (A, B, C, D, etc...) I doubt that the physical arrangement of the containers affects the billing if you are still treating each core as a different specimen... ________________________________ From: Jones, Laura [mailto:lpjones@srhs-pa.org] Sent: Mon 7/11/2011 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We looked at these, but our Pathologist's first question was "can we bill for 6 or 8 bottles if they are technically all in one?" How is that handled? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Monday, July 11, 2011 11:44 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We don't currently use them but I've used them in the past. As long as you don't 'bump' them on the grossing station while they're open, they're fine. I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ________________________________ From: Sheila Adey [mailto:sadey@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Jul 13 08:14:28 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jul 13 08:14:34 2011 Subject: [Histonet] Remote Alarm for Processor Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF87C@nmdamailsvr.nmda.ad.nmsu.edu> Do you use a remote alarm on your processor? If so, would you tell me what brand/model/manufacturer and how it works (keeping in mind that I am Electronically Challenged)? I run a Leica ASP300 in a new building with only digital phone lines and zero cell phone reception inside the building. I can't think of anything else that would complicate this issue more than that! Any help out there? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From wbenton <@t> cua.md Wed Jul 13 08:19:36 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Jul 13 08:21:47 2011 Subject: [Histonet] RE: Remote Alarm for Processor In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF87C@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B051DF87C@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <0B8979A204680A42B93A52B486088CD91FC4D9A473@CUAEXH1.GCU-MD.local> You need to get an autodialer. Search for a company in your area. If you have Biomed or telecommunications in your facility they should be able to install it for you. I purchased a land line autodailer from Radio Shack years ago for three Sakura VIPs. VOIP autodialers are available as well. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Wednesday, July 13, 2011 9:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote Alarm for Processor Do you use a remote alarm on your processor? If so, would you tell me what brand/model/manufacturer and how it works (keeping in mind that I am Electronically Challenged)? I run a Leica ASP300 in a new building with only digital phone lines and zero cell phone reception inside the building. I can't think of anything else that would complicate this issue more than that! Any help out there? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From TMcNemar <@t> lmhealth.org Wed Jul 13 08:54:42 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jul 13 08:54:53 2011 Subject: [Histonet] RE: Remote Alarm for Processor In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF87C@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B051DF87C@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: We have an alarm system that monitors all of the refrigerators and freezers in the lab and our processor is tied into that. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, July 13, 2011 9:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote Alarm for Processor Do you use a remote alarm on your processor? If so, would you tell me what brand/model/manufacturer and how it works (keeping in mind that I am Electronically Challenged)? I run a Leica ASP300 in a new building with only digital phone lines and zero cell phone reception inside the building. I can't think of anything else that would complicate this issue more than that! Any help out there? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From thiggins <@t> cddmedical.com Wed Jul 13 09:06:08 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Wed Jul 13 09:06:12 2011 Subject: [Histonet] Control Tissue Message-ID: <005d01cc4166$03bd9ed0$e001a8c0@cdd.loc> My apologies, I wasn't ready for the number of responses I received over the control tissue and might have missed some people. If I didn't contact you back shoot me another email so I can start working on it. Control Tissue still Available Iron/FE Tonsil Hpylori CD15/30 TTF1 p16 AFB Fungus I will need is your: FedEx or UPS # Name Department Address Phone # Thanks, Tim From ftryka <@t> tetonhospital.org Wed Jul 13 10:29:55 2011 From: ftryka <@t> tetonhospital.org (Tryka, A. Francine) Date: Wed Jul 13 10:30:02 2011 Subject: [Histonet] alcohol as a fixative Message-ID: Hello all in histoland. I have a clinician who may be sensitive to formalin. One of my colleagues suggests that he place his endoscopy specimens into alcohol instead of the usual formalin to mitigate his exposure. Have any of you dealt with this problem? What percent alcohol would you suggest? Or methanol? Would you transfer it back into formalin upon receipt in the lab? Thanks, Franci Tryka ________________________________ Correspondence, including e-mail and other electronic communications, to and from employees and elected officials of the Teton County Hospital District, dba St. John's Medical Center, may be subject to disclosure under the Wyoming Public Records Act. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender by reply e-mail and destroy all copies of the original message. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Wed Jul 13 10:52:34 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 13 10:52:42 2011 Subject: [Histonet] alcohol as a fixative In-Reply-To: Message-ID: <1310572354.81035.YahooMailClassic@web65703.mail.ac4.yahoo.com> If? the the biopsies are placed in alcohol, they will be fixed in alcohol and it is in no way advisable to place them in NBF after wards. It will be a double and almost incompatible fixation that most likely will ruin the specimens affecting their processing and ulterior staining procedures (specially IHC).. If you clinician "may be sensitive" to formalin he can use, besides the gloves, a mask. You are endangering the specimens and I think that this?ought be your (and your pathologist) main concern. I think that a small "sacrifice" by the clinician is the least the patient deserves to assure a correctly fixed biopsy. Ren? J. --- On Wed, 7/13/11, Tryka, A. Francine wrote: From: Tryka, A. Francine Subject: [Histonet] alcohol as a fixative To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, July 13, 2011, 11:29 AM Hello all in histoland.? I have a clinician who may be sensitive to formalin.? One of my colleagues suggests that he place his endoscopy specimens into alcohol instead of the usual formalin to mitigate his exposure.???Have any of you dealt with this problem?? What percent alcohol would you suggest?? Or methanol?? Would you transfer it back into formalin upon receipt in the lab?? Thanks, Franci Tryka ________________________________ Correspondence, including e-mail and other electronic communications, to and from employees and elected officials of the Teton County Hospital District, dba St. John's Medical Center, may be subject to disclosure under the Wyoming Public Records Act. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender by reply e-mail and destroy all copies of the original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From k84as <@t> yahoo.com Wed Jul 13 10:53:24 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Wed Jul 13 10:53:29 2011 Subject: [Histonet] scholarship Message-ID: <1310572404.53072.YahooMailClassic@web112606.mail.gq1.yahoo.com> hi all i'm going to apply for PhD degree in using educational technology in Histology teaching. do you know any university i could contact for acceptance letter? i have my funded scholarship ? thanx From cpyse <@t> x-celllab.com Wed Jul 13 11:08:21 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Jul 13 11:08:25 2011 Subject: [Histonet] alcohol as a fixative In-Reply-To: References: Message-ID: <001601cc4177$170c2f90$45248eb0$@com> Most clinician I know have other staff in the procedure room. Why not have someone other than the clinician place the specimen in the formalin. This way you get the best possible fixation for the patient. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tryka, A. Francine Sent: Wednesday, July 13, 2011 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcohol as a fixative Hello all in histoland. I have a clinician who may be sensitive to formalin. One of my colleagues suggests that he place his endoscopy specimens into alcohol instead of the usual formalin to mitigate his exposure. Have any of you dealt with this problem? What percent alcohol would you suggest? Or methanol? Would you transfer it back into formalin upon receipt in the lab? Thanks, Franci Tryka ________________________________ Correspondence, including e-mail and other electronic communications, to and from employees and elected officials of the Teton County Hospital District, dba St. John's Medical Center, may be subject to disclosure under the Wyoming Public Records Act. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender by reply e-mail and destroy all copies of the original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Jul 13 11:20:07 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jul 13 11:20:07 2011 Subject: [Histonet] RELIA Histology Careers Bulletin 7-13-2011 I hope you are having a great Summer!! Message-ID: <158E6810365F4D3FAD9C1653BDBD4F82@ownerf1abaad51> Hi Histonetters!! What are you doing on your summer vacation? Been anyplace you would like to move to? Closer to the beach or the mountains? Closer to family or friends? I can help!! There is still time this summer to make that move. I have opportunities in all kinds of labs in all areas of the country. Your next position is just a phone call or an e-mail away. All of the opportunities that I represent are permanent full time positions with companies that offer excellent salaries, benefits and relocation assistance. Here are some of the opportunities that I am most excited about: HISTOLOGY SUPERVISORS AND MANAGERS: LA - Histology Supervisor ? Baton Rouge, LA MA ? Night Shift Supervisor ? Boston area TN ? Histology Supervisor ? Nashville, TN OR ? Immunohistochemistry Lead Tech/Supervisor - Portland HISTOTECHNICIANS/HISTOTECHNOLOGISTS NC ? Grossing Histotechnologist-Charlotte MD ? Grossing Histotechnologist - Baltimore TX ? Histology Tech - Tyler LA ? Night Shift Histology Tech - Lafayette NC ? Day shift - Asheville NY ? Day shift ? Long Island OR ? Histotechnician/Histotechnologist ? Portland FL ? Mohs Histotech ? Sarasota MA ? Day shift ? Cape Cod CA- Histotechnologist - Valencia If you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1@earthlink.net or give me a call toll free at 866-607-3542. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From katelin09htl <@t> gmail.com Wed Jul 13 12:42:25 2011 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Wed Jul 13 12:42:30 2011 Subject: [Histonet] Quick Drying Mounting Media Message-ID: Hi histonet, We have a brand new Leica CV5030 Coverslipper and I am looking for the quickest drying media available. We are currently using Micromount by Surgipath/Leica and it is not drying fast enough. Any suggestions are appreciated as we have to package our slides for shipping and the media is either sticking to the bubble wrap or creating circles on the slides from the pressure of the bubble wrap. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 From katelin09htl <@t> gmail.com Wed Jul 13 13:14:01 2011 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Wed Jul 13 13:14:06 2011 Subject: [Histonet] Quick Drying Mounting Media In-Reply-To: <4E1D9478.E948.00AC.1@mercyhealth.com> References: <4E1D9478.E948.00AC.1@mercyhealth.com> Message-ID: We are shipping them out same day. At this point, if I can not find a quicker drying media I will have to delay the shipments by one day. What temperature do you dry them at? On Wed, Jul 13, 2011 at 10:50 AM, Marcia Funk wrote: > Katelin- do you ship the dame day ? We dry our slides in a oven daily > and file > ship the next day . seems to work > Marcia > > Marcia Funk > Histology Laboratory > Mercy Medical Center North Iowa > Mason City, IA, 50401 > 641-428-7907 > > > >>> Katelin Lester 7/13/2011 12:42 PM >>> > > Hi histonet, > We have a brand new Leica CV5030 Coverslipper and I am looking for the > quickest drying media available. We are currently using Micromount by > Surgipath/Leica and it is not drying fast enough. > Any suggestions are appreciated as we have to package our slides for > shipping and the media is either sticking to the bubble wrap or creating > circles on the slides from the pressure of the bubble wrap. > > > -- > Katelin Lester, HTL > Gastroenterology Specialists of Oregon, P.C. > Pathology Laboratory > (971) 224-2408 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 From jclark <@t> pcnm.com Wed Jul 13 13:22:17 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Jul 13 13:22:22 2011 Subject: [Histonet] IHC for H. pylori Message-ID: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> Hi All, was wondering how everyone does their IHC for H. pylori. Do you automatically run it on all biopsies of the stomach, or do you screen first with a giemsa and run IHC on the ones that are negative by giemsa? Thanks for the input! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico From foreightl <@t> gmail.com Wed Jul 13 13:29:24 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Wed Jul 13 13:29:32 2011 Subject: [Histonet] IHC for H. pylori In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> Message-ID: Our pathology groups decision was to run IHC for H.pylori on all stomach biopsies. On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark wrote: > Hi All, was wondering how everyone does their IHC for H. pylori. Do you > automatically run it on all biopsies of the stomach, or do you screen first > with a giemsa and run IHC on the ones that are negative by giemsa? Thanks > for the input! > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From hrfulklab <@t> gmail.com Wed Jul 13 13:29:30 2011 From: hrfulklab <@t> gmail.com (H R) Date: Wed Jul 13 13:29:36 2011 Subject: [Histonet] QC vs QA Message-ID: Working on my procedure manual for CLIA and was wondering what the difference is between QC procedures and QA procedures.? From amber.mckenzie <@t> gastrodocs.net Wed Jul 13 13:32:08 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Jul 13 13:35:54 2011 Subject: [Histonet] QC vs QA In-Reply-To: References: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B370448A5F8@giamail2.Gia.com> If you only need to keep QC records for 2 years, is it the same for QA records? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R Sent: Wednesday, July 13, 2011 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC vs QA Working on my procedure manual for CLIA and was wondering what the difference is between QC procedures and QA procedures.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Jul 13 13:37:50 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 13 13:37:59 2011 Subject: [Histonet] IHC for H. pylori In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> Message-ID: <4E1DADBE.7400.0077.1@harthosp.org> We only run IHC for H. pylori when the appropriate inflammatory background is present or when the patient has tested positive in the past and we receive a follow-up gastric specimen where bugs are not identified on H&E. Running H. pylori IHC on every gastric biopsy is uncalled for and borders on "Fraud and Abuse". Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Joanne Clark 7/13/2011 2:22 PM >>> Hi All, was wondering how everyone does their IHC for H. pylori. Do you automatically run it on all biopsies of the stomach, or do you screen first with a giemsa and run IHC on the ones that are negative by giemsa? Thanks for the input! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Jul 13 13:47:13 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jul 13 13:47:21 2011 Subject: [Histonet] IHC for H. pylori In-Reply-To: <4E1DADBE.7400.0077.1@harthosp.org> References: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> <4E1DADBE.7400.0077.1@harthosp.org> Message-ID: We run the H. pylori IHC stain on all stomach specimens to improve turn around time; however, when an inflammatory background is not appreciated by the pathologist the charge is removed before sign-out and we eat the cost of producing the slide. Mark On Wed, Jul 13, 2011 at 11:37 AM, Richard Cartun wrote: > We only run IHC for H. pylori when the appropriate inflammatory background > is present or when the patient has tested positive in the past and we > receive a follow-up gastric specimen where bugs are not identified on H&E. > > Running H. pylori IHC on every gastric biopsy is uncalled for and borders > on "Fraud and Abuse". > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> Joanne Clark 7/13/2011 2:22 PM >>> > Hi All, was wondering how everyone does their IHC for H. pylori. Do you > automatically run it on all biopsies of the stomach, or do you screen first > with a giemsa and run IHC on the ones that are negative by giemsa? Thanks > for the input! > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Wed Jul 13 14:11:29 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jul 13 14:11:34 2011 Subject: [Histonet] RELIA Hot Management Job Alert Anatomic Pathology Lab Manager for top Lab in Austin, TX Message-ID: <756593A6AD9F49EFA099D740BFD653AB@ownerf1abaad51> Hi Histonetters!! Here is a an exciting opportunity HOT OFF THE PRESSES! Anatomic Laboratory Manager ? Leading Diagnostic Lab ? Austin, TX RELIA Solutions the nation?s only recruiting firm specializing in the permanent placement of histology professionals has been engaged by a leading Pathology Lab in Austin, TX to assist in their search for an Anatomic Pathology Laboratory Manager. My client offers a very competitive compensation package including bonuses and relocation assistance along with an incredible benefits package. This person will be responsible for the overall daily and financial operation of the Histology and Cytology Departments. Direct supervision of their department supervisors, delegating responsibilities as appropriate to these General Supervisors. Working directly in support of the Technical supervisors for these areas. As well as developing or approving department specific policies. Participating in the development and approval of laboratory wide policies and procedures; ensuring that all laboratory policies and procedures are followed; accepting and supporting activities delegated by the Medical Director and Vice President of Operations. Qualifications: 1.Bachelors or equivalent degree in the sciences. 2.Minimum 10 years experience in laboratory operations. 3.ASCP certification or equivalent for testing areas. 4.Leadership skills and supervisory abilities. 5.Organize and coordinate diverse activities. 6.Continuous improvement experience For more information contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Keywords histology histologist anatomic pathology cytology Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From pauls <@t> ncimicro.com Wed Jul 13 14:14:18 2011 From: pauls <@t> ncimicro.com (Paul Scholder) Date: Wed Jul 13 14:14:22 2011 Subject: [Histonet] Nbldsu Message-ID: From mtitford <@t> aol.com Wed Jul 13 14:18:55 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jul 13 14:19:19 2011 Subject: [Histonet] And now, IHC playing cards! Message-ID: <8CE0FA8D804645B-2794-3F7C@webmail-d180.sysops.aol.com> Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma coffee mugs) will be amused to learn that Cell Marque are giving away packs of playing cards. Each card shows a section stained with a different antobody (one of theirs of course, together with the catalogue number). No, I don't have shares in Cell Marque, but I am continually amused by the different methods companies in all walks of life use to advertise their products. Some are, well, just original! Michael Titford Pathology USA Mobile AL USA From dbpiontek <@t> hotmail.com Wed Jul 13 14:23:32 2011 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Wed Jul 13 14:25:23 2011 Subject: [Histonet] Re: Message-ID: It?s the most powerful treatment I?ve ever used!... http://israelnlp.com/inbox.php?epyahooID=76n4 From Rcartun <@t> harthosp.org Wed Jul 13 14:27:55 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 13 14:28:02 2011 Subject: [Histonet] Question - Anatomic Pathology Manager Message-ID: <4E1DB97B.7400.0077.1@harthosp.org> How many Hospital-based Pathology Departments with 55,000 surgical specimens, 100,000 PAPs, and 6,000 non-GYN cytology cases have separate managers for Cytology and Histology? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From ASelf <@t> georgetownhospitalsystem.org Wed Jul 13 14:42:38 2011 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Wed Jul 13 14:42:43 2011 Subject: [Histonet] Tzank Smears Message-ID: Hello Histonetters, I was wandering if anyone had a SOP for the collection and processing/staining of tzank smears that they would be willing to share with me? Thanks in advance for everyone's help. Amy Self Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From laurie.colbert <@t> huntingtonhospital.com Wed Jul 13 14:46:31 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jul 13 14:46:35 2011 Subject: [Histonet] SlideMate Procedure Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AE7A@EXCHANGE3.huntingtonhospital.com> Does anyone have a PSLIM/SlideMate procedure that they would be willing to share with me? Laurie Colbert From Fawn.Bomar <@t> HalifaxRegional.com Wed Jul 13 15:07:01 2011 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Wed Jul 13 15:07:19 2011 Subject: [Histonet] IHC for H. pylori In-Reply-To: References: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan>, Message-ID: We run giemsa's on all stomach bx's ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreightl@gmail.com] Sent: Wednesday, July 13, 2011 2:29 PM To: Joanne Clark Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC for H. pylori *** WARNING *** This email has already passed extensive Anti-Spam and Anti-Virus scanning measures and found to have a high probability of not containing spam or virus content. However... This email has failed Domain Keys Identified Mail (DKIM) verification. 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If you need further information concerning this classification, please contact CareBridge Support with the below information: Filter: ib_dkim_fail Policy: $THROTTLED-conn_accept Time: 14:30:17 Message ID: 56429152 Envelope From: histonet-bounces@lists.utsouthwestern.edu Envelope Recipients: donna.hunter@northside.com, terri.brown@northside.com, fawn.bomar@halifaxregional.com From IP: swlx162.swmed.edu --- Headers --- From histonet-bounces@lists.utsouthwestern.edu Wed Jul 13 14:30:17 2011 X-IronPort-RCPT-TO: donna.hunter@northside.com X-IronPort-RCPT-TO: terri.brown@northside.com X-IronPort-RCPT-TO: fawn.bomar@halifaxregional.com Received: from swlx162.swmed.edu ([199.165.152.162]) by ironport2.carebridge.net with ESMTP; 13 Jul 2011 14:30:16 -0400 Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Qh4Bh-0004Ss-Ai; Wed, 13 Jul 2011 13:29:33 -0500 Received: from [199.242.239.114] (helo=swlxmx2.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Qh4Be-0004Sn-Bw for histonet@lists.utsouthwestern.edu; Wed, 13 Jul 2011 13:29:30 -0500 Received: from mail-gy0-f170.google.com ([209.85.160.170]) by swlxmx2.swmed.edu with esmtps (TLSv1:RC4-SHA:128) (Exim 4.76) (envelope-from ) id 1Qh4BZ-0003KS-0v for histonet@lists.utsouthwestern.edu; Wed, 13 Jul 2011 13:29:30 -0500 Received: by gyb13 with SMTP id 13so4681932gyb.1 for ; Wed, 13 Jul 2011 11:29:24 -0700 (PDT) DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=gmail.com; s=gamma; h=mime-version:in-reply-to:references:date:message-id:subject:from:to :cc:content-type; bh=lOG2hfqGVG6xcFwEvpzyuF7pMx4zd9N+bkiO1fp2Hu4=; b=lMlgbSyOHSzOkPu4h9jvfu3Tthsx82aI3mQrNy2pGaByT6kLNNTbnKGb4YTCMT9JN/ OtZItQexDVs9Vj2s0qj0TsYSMvL5Te3b4nvutmrrdzV5obG20Cs40cn0Pvx6xEb3QhXO 80KaRKs+mQ4pE8cp6U38LCIZYowV53YdafTK4= MIME-Version: 1.0 Received: by 10.101.214.10 with SMTP id r10mr1372056anq.115.1310581764172; Wed, 13 Jul 2011 11:29:24 -0700 (PDT) Received: by 10.100.110.18 with HTTP; Wed, 13 Jul 2011 11:29:24 -0700 (PDT) In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> Date: Wed, 13 Jul 2011 11:29:24 -0700 Message-ID: From: Patrick Laurie To: Joanne Clark X-Scan-Signature: e86858519762891a1be11808e8cb03b9 X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on swlxmx2.swmed.edu X-Spam-Level: X-Spam-Status: No, score=-0.6 required=5.0 tests=DKIM_SIGNED,DKIM_VALID, DKIM_VALID_AU, FREEMAIL_FROM, HTML_MESSAGE, J_CHICKENPOX_16, RCVD_IN_DNSWL_LOW, SPF_PASS autolearn=disabled version=3.3.1 X-Spam-Relay-Country: US Subject: Re: [Histonet] IHC for H. pylori Content-Type: text/plain; charset=ISO-8859-1 X-Content-Filtered-By: Mailman/MimeDel 2.1.5 Cc: "histonet@lists.utsouthwestern.edu" X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.1.5 Precedence: list List-Id: For the exchange of information pertaining to histotechnology and related fields List-Unsubscribe: , List-Archive: List-Post: List-Help: List-Subscribe: , Sender: histonet-bounces@lists.utsouthwestern.edu Errors-To: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: f1c10eec54d1b063b9d41be68e9394bc X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false ************* Received Email Follows ************* Our pathology groups decision was to run IHC for H.pylori on all stomach biopsies. On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark wrote: > Hi All, was wondering how everyone does their IHC for H. pylori. Do you > automatically run it on all biopsies of the stomach, or do you screen first > with a giemsa and run IHC on the ones that are negative by giemsa? Thanks > for the input! > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From LSebree <@t> uwhealth.org Wed Jul 13 15:27:48 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Jul 13 15:28:16 2011 Subject: [Histonet] Reference labs doing Fast Myosin IHC stain Message-ID: I'm looking for a reference lab that can do Fast Myosin immunohistochemistry staining on FFPE human tissue. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From nelsonrnch <@t> verizon.net Wed Jul 13 15:38:17 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Wed Jul 13 15:38:20 2011 Subject: [Histonet] QC vs QA In-Reply-To: References: Message-ID: <1310589497.49973.YahooMailRC@web84308.mail.re1.yahoo.com> Quality Assurance outlines all required?QC activities used to identify policies or procedures that are not working as desired, especially those that are not immediately apparent from following routine QC procedures. The purspose of?a QA?plan is effectively achieved by providing a mechanism for identifying problems so that corrective actions can be taken to ensure, reliable, and prompt reporting of patient test results. Quality?Control is to assess the effectiveness of the quality control system to identify inaccuracies in?test systems prior to result reporting. To verify the?proper use?and evaluation of quality control data by testing personnel. To assess test methodologies based upon past QC results. ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ________________________________ From: H R To: histonet@lists.utsouthwestern.edu Sent: Wed, July 13, 2011 11:29:30 AM Subject: [Histonet] QC vs QA Working on my procedure manual for CLIA and was wondering what the difference is between QC procedures and QA procedures.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hctrupath <@t> att.net Wed Jul 13 15:41:46 2011 From: hctrupath <@t> att.net (Heather Cooper) Date: Wed Jul 13 15:41:49 2011 Subject: [Histonet] Bone Marrow Biopsies Message-ID: <1310589706.46384.YahooMailRC@web80013.mail.sp1.yahoo.com> What is the procedure for collecting and processing bone marrow bx? How much time should it be placed in DECAL?????????????? From Marilyn.A.Weiss <@t> kp.org Wed Jul 13 18:02:55 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Jul 13 18:03:28 2011 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 07/13/2011 and will not return until 07/18/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From andreahooper <@t> rocketmail.com Wed Jul 13 22:10:16 2011 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Jul 13 22:10:23 2011 Subject: [Histonet] Antigen retrieval survey In-Reply-To: Message-ID: <1310613016.98546.YahooMailClassic@web113107.mail.gq1.yahoo.com> Hi All, ? I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite?antigen retrieval method and/or panel? ? Buffer Source/composition Temperature Device ? Thanks, Andrea From andreahooper <@t> rocketmail.com Wed Jul 13 22:13:43 2011 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Jul 13 22:13:47 2011 Subject: [Histonet] confocal questions: mixing fluorescent dyes with proteins In-Reply-To: Message-ID: <1310613223.80664.YahooMailClassic@web113107.mail.gq1.yahoo.com> It will depend on how your scope is configured. On our system, when using the three you have outlined I routinely use CY5 or Alexa633 (or equivalent in far red category)?on our scope. It's actually my personal favorite. If you have any doubts check out the spectral viewer on Invitrogen and also BD Bioscience's website. --- On Wed, 7/13/11, Tyrone Genade wrote: From: Tyrone Genade Subject: [Histonet] confocal questions: mixing fluorescent dyes with proteins To: "histonet" Date: Wednesday, July 13, 2011, 11:23 AM Hello, I'm working on a Zeiss confocal microscope. We use chiefly Alexa488 and Cy3 together with Hoechst 33258. Can anyone suggest a fluorescent protein which can be discerned amongst the Alexa488, Cy3 and Hoechst 33258 emission spectra? I was thinking maybe mBanana? Thanks -- Tyrone Genade email: tgenade@gmail.com tel: +27-84-632-1925 (c) Department of Human Biology University of Cape Town South Africa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Wed Jul 13 22:17:03 2011 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Jul 13 22:17:11 2011 Subject: [Histonet] In search of a cryostat... In-Reply-To: <1310313347.81123.YahooMailClassic@web65713.mail.ac4.yahoo.com> Message-ID: <1310613423.83362.YahooMailClassic@web113106.mail.gq1.yahoo.com> Agree, switching to Leica with disposable blade holder changed my life. Never looked back. Love the CM3050S. --- On Sun, 7/10/11, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] In search of a cryostat... To: "histonet@lists.utsouthwestern.edu" , "Tom McNemar" Date: Sunday, July 10, 2011, 3:55 PM Buy Leica Ren? J. --- On Sun, 7/10/11, Tom McNemar wrote: From: Tom McNemar Subject: [Histonet] In search of a cryostat... To: "histonet@lists.utsouthwestern.edu" Date: Sunday, July 10, 2011, 10:53 AM Hello all, I am looking to budget for a new cryostat and was wondering what others are using.? I would like one that is safe, well lit, roomy, and has some sort of on-board decontamination routine.? All input appreciated.? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Wed Jul 13 22:40:16 2011 From: andreahooper <@t> rocketmail.com (Andrea T. Hooper) Date: Wed Jul 13 22:40:23 2011 Subject: [Histonet] Anti-RFP Message-ID: <1310614816.57254.YahooMailClassic@web113116.mail.gq1.yahoo.com> Is anyone currently staining for RFP in FFPE or frozen sections using a detecting antibody? I am curious as to what anti-RFP Ab would be recommended. There is one I plan to try from Invitrogen (rabbit anti-RFP) but of course would love feedback if people have used one in particular. ? Thanks, Andrea From gu.lang <@t> gmx.at Thu Jul 14 02:57:55 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jul 14 02:58:02 2011 Subject: AW: [Histonet] Quick Drying Mounting Media In-Reply-To: References: Message-ID: I think it's more depending on the organic solvent used before coverslipping. We use butyl acetate in the last trogh in the stainer and in the coverslipper. This reagent dries/ evaporates very fast. So after 15 min there is hardly a smell of it and we deliver our slides immediately. We use Pertex as coverslipping medium. Gudrun Histolab, Akh Linz, Austria -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Katelin Lester Gesendet: Mittwoch, 13. Juli 2011 19:42 An: Histonet Betreff: [Histonet] Quick Drying Mounting Media Hi histonet, We have a brand new Leica CV5030 Coverslipper and I am looking for the quickest drying media available. We are currently using Micromount by Surgipath/Leica and it is not drying fast enough. Any suggestions are appreciated as we have to package our slides for shipping and the media is either sticking to the bubble wrap or creating circles on the slides from the pressure of the bubble wrap. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shehnazster <@t> gmail.com Thu Jul 14 04:21:10 2011 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Thu Jul 14 04:21:15 2011 Subject: [Histonet] specimen container analytic interference In-Reply-To: References: Message-ID: Hi Netters, Please shed some light on the ffg: specimen container analytic interference. What should I do to comply with this standard in the histo - cyto setting? Thanx again S Kahn From histotech <@t> imagesbyhopper.com Thu Jul 14 05:20:03 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Jul 14 05:20:16 2011 Subject: AW: [Histonet] Quick Drying Mounting Media In-Reply-To: References: Message-ID: <4A2541E6-2820-438A-9374-F3480E1246A4@imagesbyhopper.com> Have you tried drying them under a "blow dryer"? That's how we used to dry our slides before we sent them out. It was a commercially available slide dryer. Michelle Sent from my iPhone On Jul 14, 2011, at 3:57 AM, "Gudrun Lang" wrote: > I think it's more depending on the organic solvent used before > coverslipping. > We use butyl acetate in the last trogh in the stainer and in the > coverslipper. This reagent dries/ evaporates very fast. So after 15 min > there is hardly a smell of it and we deliver our slides immediately. > We use Pertex as coverslipping medium. > > Gudrun > Histolab, Akh Linz, Austria > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Katelin > Lester > Gesendet: Mittwoch, 13. Juli 2011 19:42 > An: Histonet > Betreff: [Histonet] Quick Drying Mounting Media > > Hi histonet, > We have a brand new Leica CV5030 Coverslipper and I am looking for the > quickest drying media available. We are currently using Micromount by > Surgipath/Leica and it is not drying fast enough. > Any suggestions are appreciated as we have to package our slides for > shipping and the media is either sticking to the bubble wrap or creating > circles on the slides from the pressure of the bubble wrap. > > > -- > Katelin Lester, HTL > Gastroenterology Specialists of Oregon, P.C. > Pathology Laboratory > (971) 224-2408 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Thu Jul 14 08:46:17 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jul 14 08:46:21 2011 Subject: [Histonet] And now, IHC playing cards! In-Reply-To: <8CE0FA8D804645B-2794-3F7C@webmail-d180.sysops.aol.com> References: <8CE0FA8D804645B-2794-3F7C@webmail-d180.sysops.aol.com> Message-ID: Do you have to buy anything to get them? Or do you just go to their website? A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, wrote: > > > Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma > coffee mugs) will be amused to learn that Cell Marque are giving away packs > of playing cards. Each card shows a section stained with a different > antobody (one of theirs of course, together with the catalogue number). > > No, I don't have shares in Cell Marque, but I am continually amused by the > different methods companies in all walks of life use to advertise their > products. Some are, well, just original! > > Michael Titford > Pathology USA > Mobile AL USA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Thu Jul 14 09:04:54 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jul 14 09:04:57 2011 Subject: [Histonet] And now, IHC playing cards! In-Reply-To: <8CE0FA8D804645B-2794-3F7C@webmail-d180.sysops.aol.com> References: <8CE0FA8D804645B-2794-3F7C@webmail-d180.sysops.aol.com> Message-ID: Hello again! I went to their site and can't find anything...hopefully I didn't miss it!! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, wrote: > > > Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma > coffee mugs) will be amused to learn that Cell Marque are giving away packs > of playing cards. Each card shows a section stained with a different > antobody (one of theirs of course, together with the catalogue number). > > No, I don't have shares in Cell Marque, but I am continually amused by the > different methods companies in all walks of life use to advertise their > products. Some are, well, just original! > > Michael Titford > Pathology USA > Mobile AL USA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Thu Jul 14 09:06:27 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 14 09:06:30 2011 Subject: [Histonet] Antigen retrieval survey In-Reply-To: <1310613016.98546.YahooMailClassic@web113107.mail.gq1.yahoo.com> Message-ID: <1310652387.83279.YahooMailClassic@web65711.mail.ac4.yahoo.com> Citrate buffer Ren? J. --- On Wed, 7/13/11, Andrea T. Hooper wrote: From: Andrea T. Hooper Subject: [Histonet] Antigen retrieval survey To: "Histonet" Date: Wednesday, July 13, 2011, 11:10 PM Hi All, ? I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite?antigen retrieval method and/or panel? ? Buffer Source/composition Temperature Device ? Thanks, Andrea _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thiggins <@t> cddmedical.com Thu Jul 14 09:09:56 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Thu Jul 14 09:10:05 2011 Subject: [Histonet] Re: Histonet Digest, Vol 92, Issue 18 References: <20110714034501.B26DD12DADB1@barracuda.crvinc.net> Message-ID: <001d01cc422f$b643e220$e001a8c0@cdd.loc> On all stomach biopsies. Tim ----- Original Message ----- From: To: Sent: Wednesday, July 13, 2011 10:45 PM Subject: Histonet Digest, Vol 92, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Quick Drying Mounting Media (Katelin Lester) 2. Re: Quick Drying Mounting Media (Katelin Lester) 3. IHC for H. pylori (Joanne Clark) 4. Re: IHC for H. pylori (Patrick Laurie) 5. QC vs QA (H R) 6. RE: QC vs QA (Amber McKenzie) 7. Re: IHC for H. pylori (Richard Cartun) 8. Re: IHC for H. pylori (Mark Tarango) 9. RELIA Hot Management Job Alert Anatomic Pathology Lab Manager for top Lab in Austin, TX (Pam Barker) 10. Nbldsu (Paul Scholder) 11. And now, IHC playing cards! (mtitford@aol.com) 12. Re: (Denise Piontek) 13. Question - Anatomic Pathology Manager (Richard Cartun) 14. Tzank Smears (Amy Self) 15. SlideMate Procedure (Laurie Colbert) 16. RE: IHC for H. pylori (Fawn Bomar) 17. Reference labs doing Fast Myosin IHC stain (Sebree Linda A) 18. Re: QC vs QA (SHANE NELSON) 19. Bone Marrow Biopsies (Heather Cooper) 20. out of office (Marilyn.A.Weiss@kp.org) 21. Antigen retrieval survey (Andrea T. Hooper) 22. Re: confocal questions: mixing fluorescent dyes with proteins (Andrea T. Hooper) 23. Re: In search of a cryostat... (Andrea T. Hooper) 24. Anti-RFP (Andrea T. Hooper) ---------------------------------------------------------------------- Message: 1 Date: Wed, 13 Jul 2011 10:42:25 -0700 From: Katelin Lester Subject: [Histonet] Quick Drying Mounting Media To: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi histonet, We have a brand new Leica CV5030 Coverslipper and I am looking for the quickest drying media available. We are currently using Micromount by Surgipath/Leica and it is not drying fast enough. Any suggestions are appreciated as we have to package our slides for shipping and the media is either sticking to the bubble wrap or creating circles on the slides from the pressure of the bubble wrap. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 ------------------------------ Message: 2 Date: Wed, 13 Jul 2011 11:14:01 -0700 From: Katelin Lester Subject: Re: [Histonet] Quick Drying Mounting Media To: Marcia Funk , Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We are shipping them out same day. At this point, if I can not find a quicker drying media I will have to delay the shipments by one day. What temperature do you dry them at? On Wed, Jul 13, 2011 at 10:50 AM, Marcia Funk wrote: > Katelin- do you ship the dame day ? We dry our slides in a oven daily > and file > ship the next day . seems to work > Marcia > > Marcia Funk > Histology Laboratory > Mercy Medical Center North Iowa > Mason City, IA, 50401 > 641-428-7907 > > > >>> Katelin Lester 7/13/2011 12:42 PM >>> > > Hi histonet, > We have a brand new Leica CV5030 Coverslipper and I am looking for the > quickest drying media available. We are currently using Micromount by > Surgipath/Leica and it is not drying fast enough. > Any suggestions are appreciated as we have to package our slides for > shipping and the media is either sticking to the bubble wrap or creating > circles on the slides from the pressure of the bubble wrap. > > > -- > Katelin Lester, HTL > Gastroenterology Specialists of Oregon, P.C. > Pathology Laboratory > (971) 224-2408 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 ------------------------------ Message: 3 Date: Wed, 13 Jul 2011 18:22:17 +0000 From: Joanne Clark Subject: [Histonet] IHC for H. pylori To: "histonet@lists.utsouthwestern.edu" Message-ID: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> Content-Type: text/plain; charset="us-ascii" Hi All, was wondering how everyone does their IHC for H. pylori. Do you automatically run it on all biopsies of the stomach, or do you screen first with a giemsa and run IHC on the ones that are negative by giemsa? Thanks for the input! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 4 Date: Wed, 13 Jul 2011 11:29:24 -0700 From: Patrick Laurie Subject: Re: [Histonet] IHC for H. pylori To: Joanne Clark Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Our pathology groups decision was to run IHC for H.pylori on all stomach biopsies. On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark wrote: > Hi All, was wondering how everyone does their IHC for H. pylori. Do you > automatically run it on all biopsies of the stomach, or do you screen first > with a giemsa and run IHC on the ones that are negative by giemsa? Thanks > for the input! > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com ------------------------------ Message: 5 Date: Wed, 13 Jul 2011 14:29:30 -0400 From: H R Subject: [Histonet] QC vs QA To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Working on my procedure manual for CLIA and was wondering what the difference is between QC procedures and QA procedures.? ------------------------------ Message: 6 Date: Wed, 13 Jul 2011 13:32:08 -0500 From: "Amber McKenzie" Subject: RE: [Histonet] QC vs QA To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B370448A5F8@giamail2.Gia.com> Content-Type: text/plain; charset="us-ascii" If you only need to keep QC records for 2 years, is it the same for QA records? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R Sent: Wednesday, July 13, 2011 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC vs QA Working on my procedure manual for CLIA and was wondering what the difference is between QC procedures and QA procedures.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 13 Jul 2011 14:37:50 -0400 From: "Richard Cartun" Subject: Re: [Histonet] IHC for H. pylori To: "histonet@lists.utsouthwestern.edu" , "Joanne Clark" Message-ID: <4E1DADBE.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII We only run IHC for H. pylori when the appropriate inflammatory background is present or when the patient has tested positive in the past and we receive a follow-up gastric specimen where bugs are not identified on H&E. Running H. pylori IHC on every gastric biopsy is uncalled for and borders on "Fraud and Abuse". Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Joanne Clark 7/13/2011 2:22 PM >>> Hi All, was wondering how everyone does their IHC for H. pylori. Do you automatically run it on all biopsies of the stomach, or do you screen first with a giemsa and run IHC on the ones that are negative by giemsa? Thanks for the input! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 13 Jul 2011 11:47:13 -0700 From: Mark Tarango Subject: Re: [Histonet] IHC for H. pylori To: Richard Cartun Cc: "histonet@lists.utsouthwestern.edu" , Joanne Clark Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We run the H. pylori IHC stain on all stomach specimens to improve turn around time; however, when an inflammatory background is not appreciated by the pathologist the charge is removed before sign-out and we eat the cost of producing the slide. Mark On Wed, Jul 13, 2011 at 11:37 AM, Richard Cartun wrote: > We only run IHC for H. pylori when the appropriate inflammatory background > is present or when the patient has tested positive in the past and we > receive a follow-up gastric specimen where bugs are not identified on H&E. > > Running H. pylori IHC on every gastric biopsy is uncalled for and borders > on "Fraud and Abuse". > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> Joanne Clark 7/13/2011 2:22 PM >>> > Hi All, was wondering how everyone does their IHC for H. pylori. Do you > automatically run it on all biopsies of the stomach, or do you screen first > with a giemsa and run IHC on the ones that are negative by giemsa? Thanks > for the input! > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Wed, 13 Jul 2011 15:11:29 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Hot Management Job Alert Anatomic Pathology Lab Manager for top Lab in Austin, TX To: "'Histonet'" Message-ID: <756593A6AD9F49EFA099D740BFD653AB@ownerf1abaad51> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! Here is a an exciting opportunity HOT OFF THE PRESSES! Anatomic Laboratory Manager - Leading Diagnostic Lab - Austin, TX RELIA Solutions the nation's only recruiting firm specializing in the permanent placement of histology professionals has been engaged by a leading Pathology Lab in Austin, TX to assist in their search for an Anatomic Pathology Laboratory Manager. My client offers a very competitive compensation package including bonuses and relocation assistance along with an incredible benefits package. This person will be responsible for the overall daily and financial operation of the Histology and Cytology Departments. Direct supervision of their department supervisors, delegating responsibilities as appropriate to these General Supervisors. Working directly in support of the Technical supervisors for these areas. As well as developing or approving department specific policies. Participating in the development and approval of laboratory wide policies and procedures; ensuring that all laboratory policies and procedures are followed; accepting and supporting activities delegated by the Medical Director and Vice President of Operations. Qualifications: 1.Bachelors or equivalent degree in the sciences. 2.Minimum 10 years experience in laboratory operations. 3.ASCP certification or equivalent for testing areas. 4.Leadership skills and supervisory abilities. 5.Organize and coordinate diverse activities. 6.Continuous improvement experience For more information contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Keywords histology histologist anatomic pathology cytology Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 10 Date: Wed, 13 Jul 2011 14:14:18 -0500 From: Paul Scholder Subject: [Histonet] Nbldsu To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 ------------------------------ Message: 11 Date: Wed, 13 Jul 2011 15:18:55 -0400 From: mtitford@aol.com Subject: [Histonet] And now, IHC playing cards! To: histonet@lists.utsouthwestern.edu Message-ID: <8CE0FA8D804645B-2794-3F7C@webmail-d180.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma coffee mugs) will be amused to learn that Cell Marque are giving away packs of playing cards. Each card shows a section stained with a different antobody (one of theirs of course, together with the catalogue number). No, I don't have shares in Cell Marque, but I am continually amused by the different methods companies in all walks of life use to advertise their products. Some are, well, just original! Michael Titford Pathology USA Mobile AL USA ------------------------------ Message: 12 Date: Wed, 13 Jul 2011 15:23:32 -0400 From: Denise Piontek Subject: [Histonet] Re: To: , , , , , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" It's the most powerful treatment I've ever used!... http://israelnlp.com/inbox.php?epyahooID=76n4 ------------------------------ Message: 13 Date: Wed, 13 Jul 2011 15:27:55 -0400 From: "Richard Cartun" Subject: [Histonet] Question - Anatomic Pathology Manager To: "Histonet" Message-ID: <4E1DB97B.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII How many Hospital-based Pathology Departments with 55,000 surgical specimens, 100,000 PAPs, and 6,000 non-GYN cytology cases have separate managers for Cytology and Histology? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 14 Date: Wed, 13 Jul 2011 15:42:38 -0400 From: Amy Self Subject: [Histonet] Tzank Smears To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonetters, I was wandering if anyone had a SOP for the collection and processing/staining of tzank smears that they would be willing to share with me? Thanks in advance for everyone's help. Amy Self Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 15 Date: Wed, 13 Jul 2011 12:46:31 -0700 From: "Laurie Colbert" Subject: [Histonet] SlideMate Procedure To: Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AE7A@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Does anyone have a PSLIM/SlideMate procedure that they would be willing to share with me? Laurie Colbert ------------------------------ Message: 16 Date: Wed, 13 Jul 2011 16:07:01 -0400 From: Fawn Bomar Subject: RE: [Histonet] IHC for H. pylori To: Patrick Laurie , Joanne Clark Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We run giemsa's on all stomach bx's ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreightl@gmail.com] Sent: Wednesday, July 13, 2011 2:29 PM To: Joanne Clark Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC for H. pylori *** WARNING *** This email has already passed extensive Anti-Spam and Anti-Virus scanning measures and found to have a high probability of not containing spam or virus content. However... This email has failed Domain Keys Identified Mail (DKIM) verification. DKIM references can be found at http://www.dkim.org and is defined by the overseeing organization as: "DomainKeys Identified Mail (DKIM) lets an organization take responsibility for a message while it is in transit. The organization is a handler of the message, either as its originator or as an intermediary. Their reputation is the basis for evaluating whether to trust the message for delivery. Technically DKIM provides a method for validating a domain name identity that is associated with a message through cryptographic authentication. " In this email, a DKIM key was supplied in the email and the DKIM cryptographic authentication has failed (with 100% certainty). Due to this, you should not necessarily trust that it was really sent from the domain stated by the sender "Patrick Laurie /histonet-bounces@lists.utsouthwestern.edu". All electronic checks have been exhausted and you will need to make a personal decision on whether to trust this sender. 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If you need further information concerning this classification, please contact CareBridge Support with the below information: Filter: ib_dkim_fail Policy: $THROTTLED-conn_accept Time: 14:30:17 Message ID: 56429152 Envelope From: histonet-bounces@lists.utsouthwestern.edu Envelope Recipients: donna.hunter@northside.com, terri.brown@northside.com, fawn.bomar@halifaxregional.com >From IP: swlx162.swmed.edu --- Headers --- From histonet-bounces@lists.utsouthwestern.edu Wed Jul 13 14:30:17 2011 X-IronPort-RCPT-TO: donna.hunter@northside.com X-IronPort-RCPT-TO: terri.brown@northside.com X-IronPort-RCPT-TO: fawn.bomar@halifaxregional.com Received: from swlx162.swmed.edu ([199.165.152.162]) by ironport2.carebridge.net with ESMTP; 13 Jul 2011 14:30:16 -0400 Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Qh4Bh-0004Ss-Ai; Wed, 13 Jul 2011 13:29:33 -0500 Received: from [199.242.239.114] (helo=swlxmx2.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Qh4Be-0004Sn-Bw for histonet@lists.utsouthwestern.edu; Wed, 13 Jul 2011 13:29:30 -0500 Received: from mail-gy0-f170.google.com ([209.85.160.170]) by swlxmx2.swmed.edu with esmtps (TLSv1:RC4-SHA:128) (Exim 4.76) (envelope-from ) id 1Qh4BZ-0003KS-0v for histonet@lists.utsouthwestern.edu; Wed, 13 Jul 2011 13:29:30 -0500 Received: by gyb13 with SMTP id 13so4681932gyb.1 for ; Wed, 13 Jul 2011 11:29:24 -0700 (PDT) DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=gmail.com; s=gamma; h=mime-version:in-reply-to:references:date:message-id:subject:from:to :cc:content-type; bh=lOG2hfqGVG6xcFwEvpzyuF7pMx4zd9N+bkiO1fp2Hu4=; b=lMlgbSyOHSzOkPu4h9jvfu3Tthsx82aI3mQrNy2pGaByT6kLNNTbnKGb4YTCMT9JN/ OtZItQexDVs9Vj2s0qj0TsYSMvL5Te3b4nvutmrrdzV5obG20Cs40cn0Pvx6xEb3QhXO 80KaRKs+mQ4pE8cp6U38LCIZYowV53YdafTK4= MIME-Version: 1.0 Received: by 10.101.214.10 with SMTP id r10mr1372056anq.115.1310581764172; Wed, 13 Jul 2011 11:29:24 -0700 (PDT) Received: by 10.100.110.18 with HTTP; Wed, 13 Jul 2011 11:29:24 -0700 (PDT) In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3786E6F61@S10MAILD001N1.SH10.lan> Date: Wed, 13 Jul 2011 11:29:24 -0700 Message-ID: From: Patrick Laurie To: Joanne Clark X-Scan-Signature: e86858519762891a1be11808e8cb03b9 X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on swlxmx2.swmed.edu X-Spam-Level: X-Spam-Status: No, score=-0.6 required=5.0 tests=DKIM_SIGNED,DKIM_VALID, DKIM_VALID_AU, FREEMAIL_FROM, HTML_MESSAGE, J_CHICKENPOX_16, RCVD_IN_DNSWL_LOW, SPF_PASS autolearn=disabled version=3.3.1 X-Spam-Relay-Country: US Subject: Re: [Histonet] IHC for H. pylori Content-Type: text/plain; charset=ISO-8859-1 X-Content-Filtered-By: Mailman/MimeDel 2.1.5 Cc: "histonet@lists.utsouthwestern.edu" X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.1.5 Precedence: list List-Id: For the exchange of information pertaining to histotechnology and related fields List-Unsubscribe: , List-Archive: List-Post: List-Help: List-Subscribe: , Sender: histonet-bounces@lists.utsouthwestern.edu Errors-To: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: f1c10eec54d1b063b9d41be68e9394bc X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false ************* Received Email Follows ************* Our pathology groups decision was to run IHC for H.pylori on all stomach biopsies. On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark wrote: > Hi All, was wondering how everyone does their IHC for H. pylori. Do you > automatically run it on all biopsies of the stomach, or do you screen first > with a giemsa and run IHC on the ones that are negative by giemsa? Thanks > for the input! > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 17 Date: Wed, 13 Jul 2011 15:27:48 -0500 From: "Sebree Linda A" Subject: [Histonet] Reference labs doing Fast Myosin IHC stain To: Message-ID: Content-Type: text/plain; charset="us-ascii" I'm looking for a reference lab that can do Fast Myosin immunohistochemistry staining on FFPE human tissue. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ------------------------------ Message: 18 Date: Wed, 13 Jul 2011 13:38:17 -0700 (PDT) From: SHANE NELSON Subject: Re: [Histonet] QC vs QA To: Histonet Message-ID: <1310589497.49973.YahooMailRC@web84308.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Quality Assurance outlines all required QC activities used to identify policies or procedures that are not working as desired, especially those that are not immediately apparent from following routine QC procedures. The purspose of a QA plan is effectively achieved by providing a mechanism for identifying problems so that corrective actions can be taken to ensure, reliable, and prompt reporting of patient test results. Quality Control is to assess the effectiveness of the quality control system to identify inaccuracies in test systems prior to result reporting. To verify the proper use and evaluation of quality control data by testing personnel. To assess test methodologies based upon past QC results. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ________________________________ From: H R To: histonet@lists.utsouthwestern.edu Sent: Wed, July 13, 2011 11:29:30 AM Subject: [Histonet] QC vs QA Working on my procedure manual for CLIA and was wondering what the difference is between QC procedures and QA procedures.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 13 Jul 2011 13:41:46 -0700 (PDT) From: Heather Cooper Subject: [Histonet] Bone Marrow Biopsies To: histonet@lists.utsouthwestern.edu Message-ID: <1310589706.46384.YahooMailRC@web80013.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii What is the procedure for collecting and processing bone marrow bx? How much time should it be placed in DECAL?????????????? ------------------------------ Message: 20 Date: Wed, 13 Jul 2011 16:02:55 -0700 From: Marilyn.A.Weiss@kp.org Subject: [Histonet] out of office To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 07/13/2011 and will not return until 07/18/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. ------------------------------ Message: 21 Date: Wed, 13 Jul 2011 20:10:16 -0700 (PDT) From: "Andrea T. Hooper" Subject: [Histonet] Antigen retrieval survey To: Histonet Message-ID: <1310613016.98546.YahooMailClassic@web113107.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All, I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite antigen retrieval method and/or panel? Buffer Source/composition Temperature Device Thanks, Andrea ------------------------------ Message: 22 Date: Wed, 13 Jul 2011 20:13:43 -0700 (PDT) From: "Andrea T. Hooper" Subject: Re: [Histonet] confocal questions: mixing fluorescent dyes with proteins To: tgenade@gmail.com Cc: Histonet Message-ID: <1310613223.80664.YahooMailClassic@web113107.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 It will depend on how your scope is configured. On our system, when using the three you have outlined I routinely use CY5 or Alexa633 (or equivalent in far red category) on our scope. It's actually my personal favorite. If you have any doubts check out the spectral viewer on Invitrogen and also BD Bioscience's website. --- On Wed, 7/13/11, Tyrone Genade wrote: From: Tyrone Genade Subject: [Histonet] confocal questions: mixing fluorescent dyes with proteins To: "histonet" Date: Wednesday, July 13, 2011, 11:23 AM Hello, I'm working on a Zeiss confocal microscope. We use chiefly Alexa488 and Cy3 together with Hoechst 33258. Can anyone suggest a fluorescent protein which can be discerned amongst the Alexa488, Cy3 and Hoechst 33258 emission spectra? I was thinking maybe mBanana? Thanks -- Tyrone Genade email: tgenade@gmail.com tel: +27-84-632-1925 (c) Department of Human Biology University of Cape Town South Africa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Wed, 13 Jul 2011 20:17:03 -0700 (PDT) From: "Andrea T. Hooper" Subject: Re: [Histonet] In search of a cryostat... To: "histonet@lists.utsouthwestern.edu" , Tom McNemar , Rene J Buesa Message-ID: <1310613423.83362.YahooMailClassic@web113106.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Agree, switching to Leica with disposable blade holder changed my life. Never looked back. Love the CM3050S. --- On Sun, 7/10/11, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] In search of a cryostat... To: "histonet@lists.utsouthwestern.edu" , "Tom McNemar" Date: Sunday, July 10, 2011, 3:55 PM Buy Leica Ren? J. --- On Sun, 7/10/11, Tom McNemar wrote: From: Tom McNemar Subject: [Histonet] In search of a cryostat... To: "histonet@lists.utsouthwestern.edu" Date: Sunday, July 10, 2011, 10:53 AM Hello all, I am looking to budget for a new cryostat and was wondering what others are using. I would like one that is safe, well lit, roomy, and has some sort of on-board decontamination routine. All input appreciated. Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 24 Date: Wed, 13 Jul 2011 20:40:16 -0700 (PDT) From: "Andrea T. Hooper" Subject: [Histonet] Anti-RFP To: Histonet Message-ID: <1310614816.57254.YahooMailClassic@web113116.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Is anyone currently staining for RFP in FFPE or frozen sections using a detecting antibody? I am curious as to what anti-RFP Ab would be recommended. There is one I plan to try from Invitrogen (rabbit anti-RFP) but of course would love feedback if people have used one in particular. Thanks, Andrea ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 18 **************************************** From TGoins <@t> mt.gov Thu Jul 14 09:38:05 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Jul 14 09:38:09 2011 Subject: [Histonet] Antigen retrieval survey In-Reply-To: <1310613016.98546.YahooMailClassic@web113107.mail.gq1.yahoo.com> References: <1310613016.98546.YahooMailClassic@web113107.mail.gq1.yahoo.com> Message-ID: I hate to be pessimistic, but different clones of monoclonal antibodies can have different optimum retrieval requirements, not to mention tissue type, source species, etc. What I do believe however, is that enzymatic retrieval is under-sold - no equipment purchase is necessary so you will not see companies advocating their use. Nearly half of the antibodies we use are optimally retrieved with enzymes (pronase, trypsin, pepsin) rather than heat retrieval. Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: Wednesday, July 13, 2011 9:10 PM To: Histonet Subject: [Histonet] Antigen retrieval survey Hi All, ? I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite?antigen retrieval method and/or panel? ? Buffer Source/composition Temperature Device ? Thanks, Andrea _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbrya <@t> lexclin.com Thu Jul 14 09:58:50 2011 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Jul 14 09:58:57 2011 Subject: [Histonet] tissue processing problem Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36ED8@EXCHANGESB> We have several tissue blocks with cells that have shrunken. The pathologist called them "shrink o cytes". The tissue affected is mostly small gastric polyps. This sounds like a dehydration issue. Any suggestions would be greatly appreciated. Thank you, Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From mw <@t> personifysearch.com Thu Jul 14 10:00:54 2011 From: mw <@t> personifysearch.com (Matt Ward) Date: Thu Jul 14 10:01:05 2011 Subject: [Histonet] New Position Alert - Field Histology Southern CA Message-ID: <5af01b5a6184e2a15b038f961e61961f@mail.gmail.com> Good Morning Everyone, New Position Alert!! One of our retained Cancer Diagnostics clients has opened up an Field Technical Support Opportunity based in Southern CA. This position is perfect for a Histotech looking to get out of the lab and into the field. Field Support Specialist - IHC The primary responsibility of this role will be to provide in-field technical applications support for current and next generation range of products. The FSS will install/set-up instrumentation in customer laboratories, perform demonstrations and maintain demonstration equipment in a clean and operational manner. The FSS will also train customers on the use of instrumentation. Conducting in-field post purchase applications support and performing instrument validations will be a key responsibility of this position. Position offers: Competitive Base Salary + Bonus/Commission + $700/Month for a Car Allowance and Gas Card, Cell Phone, Laptop, 401k, Full Benefits Open due to Promotion!! Please send your resumes to mw@personifysearch.com, or call me at 800.875.6188 ext. 103 Also, if you know of anyone who may be interested please do not hesitate to forward this e-mail, and have them reach out to me. Have a great day! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From James.Reilly <@t> glasgow.ac.uk Thu Jul 14 10:07:52 2011 From: James.Reilly <@t> glasgow.ac.uk (Jim Reilly) Date: Thu Jul 14 10:07:57 2011 Subject: [Histonet] Antigen Retrieval Message-ID: Citrate buffer is my first port of call. Jim Reilly From rjbuesa <@t> yahoo.com Thu Jul 14 10:08:33 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 14 10:08:37 2011 Subject: [Histonet] tissue processing problem In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36ED8@EXCHANGESB> Message-ID: <1310656113.97243.YahooMailClassic@web65703.mail.ac4.yahoo.com> You are right, it is most likely a dehydration issue. Try to start with 70EthOL and increase gradually until 100EthOL Ren? J. --- On Thu, 7/14/11, Carol Bryant wrote: From: Carol Bryant Subject: [Histonet] tissue processing problem To: "histonet@lists.utsouthwestern.edu" Date: Thursday, July 14, 2011, 10:58 AM We have several tissue blocks with cells that have shrunken.? The pathologist called them "shrink o cytes".???The tissue affected is mostly small gastric polyps.? This sounds like a dehydration issue.???Any suggestions would be greatly appreciated. Thank you, Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.? If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.? Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Thu Jul 14 10:44:19 2011 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Thu Jul 14 10:44:23 2011 Subject: [Histonet] Antigen retrieval survey In-Reply-To: <1310613016.98546.YahooMailClassic@web113107.mail.gq1.yahoo.com> References: <1310613016.98546.YahooMailClassic@web113107.mail.gq1.yahoo.com> Message-ID: 96 degree water bath for half an hour in either citrate buffer (pH 6) or Tris-HCl (pH 9), depending on which gives better retrieval for that antibody. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: Wednesday, July 13, 2011 11:10 PM To: Histonet Subject: [Histonet] Antigen retrieval survey Hi All, ? I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite?antigen retrieval method and/or panel? ? Buffer Source/composition Temperature Device ? Thanks, Andrea _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Thu Jul 14 10:51:18 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Thu Jul 14 10:51:27 2011 Subject: [Histonet] Autoclave water Message-ID: Hello all, We are having an issue with water (Millipore filtered and tap is the same problem). We put the water into glass pyrex jars (the kind with the orange lid) and autoclave. After they are done there are tiny little naked eye visible particles of stuff floating in the water. It shimmers and almost looks like small shards of glass? We bought new jars and it seemed to be fine for awhile, but the particles are back...any ideas?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From rsrichmond <@t> gmail.com Thu Jul 14 11:31:50 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Jul 14 11:31:53 2011 Subject: [Histonet] Re: IHC for H. pylori Message-ID: The three pathology services I've worked in in the last few yers that did immunohistochemical staining for Helicobacter pylori did it on all gastric biopsy specimens. Usually "R/O H.p." is on the requisition. I prefer a blue stain (Giemsa or Diff-Quik II or equivalent) if I have one slide a day to read, IHC if I have ten. Bob Richmond Samurai Pathologist Knoxville TN From daniel.sjolander <@t> gmail.com Thu Jul 14 11:51:19 2011 From: daniel.sjolander <@t> gmail.com (Daniel Sjolander) Date: Thu Jul 14 11:51:46 2011 Subject: [Histonet] Autoclave water In-Reply-To: References: Message-ID: Same with deionized, filtered water? /daniel On Thu, Jul 14, 2011 at 5:51 PM, wrote: > Hello all, > > We are having an issue with water (Millipore filtered and tap is the > same problem). We put the water into glass pyrex jars (the kind with > the orange lid) and autoclave. After they are done there are tiny > little naked eye visible particles of stuff floating in the water. It > shimmers and almost looks like small shards of glass? We bought new > jars and it seemed to be fine for awhile, but the particles are > back...any ideas?? > > Thanks > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From trathborne <@t> somerset-healthcare.com Thu Jul 14 11:55:16 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Jul 14 11:57:04 2011 Subject: [Histonet] Autoclave water In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB757070307C4@SMCMAIL01.somerset-healthcare.com> Have you tried glass lids? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Sjolander Sent: Thursday, July 14, 2011 12:51 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Autoclave water Same with deionized, filtered water? /daniel On Thu, Jul 14, 2011 at 5:51 PM, wrote: > Hello all, > > We are having an issue with water (Millipore filtered and tap is the > same problem). We put the water into glass pyrex jars (the kind with > the orange lid) and autoclave. After they are done there are tiny > little naked eye visible particles of stuff floating in the water. It > shimmers and almost looks like small shards of glass? We bought new > jars and it seemed to be fine for awhile, but the particles are > back...any ideas?? > > Thanks > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Mark.Elliott <@t> hli.ubc.ca Thu Jul 14 12:22:57 2011 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Thu Jul 14 12:23:20 2011 Subject: [Histonet] Re: Histonet Digest, Vol 92, Issue 19 In-Reply-To: References: Message-ID: <4E1EC381020000D600067041@mail.mrl.ubc.ca> Hello everyone. We will be starting a big IHC project on elephant hearts soon and I was wondering if there is anyone one the list who has worked with elephant tissues/hearts who might be willing to give us any hints/tips etc as we proceed. Any info/advice would be greatly appreciated. Mark in rainy Vancouver summer has only shown it's head for about a week ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Nacaela.Johnson <@t> USONCOLOGY.COM Thu Jul 14 12:55:34 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Thu Jul 14 12:55:36 2011 Subject: [Histonet] And now, IHC playing cards! In-Reply-To: References: <8CE0FA8D804645B-2794-3F7C@webmail-d180.sysops.aol.com> Message-ID: <71882EED22A283429E8424513A22922D5952B9@txhous1eb015.uson.usoncology.int> I emailed my rep and asked her to send me some. I'm studying for the QIHC and told her it would be a good study tool. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, July 14, 2011 8:46 AM To: mtitford@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] And now, IHC playing cards! Do you have to buy anything to get them? Or do you just go to their website? A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, wrote: > > > Those of you that like freebies (like Dako mousepads, Thermo pens and > Sigma coffee mugs) will be amused to learn that Cell Marque are giving > away packs of playing cards. Each card shows a section stained with a > different antobody (one of theirs of course, together with the catalogue number). > > No, I don't have shares in Cell Marque, but I am continually amused by > the different methods companies in all walks of life use to advertise > their products. Some are, well, just original! > > Michael Titford > Pathology USA > Mobile AL USA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From sbreeden <@t> nmda.nmsu.edu Thu Jul 14 13:23:14 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jul 14 13:23:19 2011 Subject: [Histonet] Lab humidity range Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF896@nmdamailsvr.nmda.ad.nmsu.edu> We're prepping for a Big Inspection (I could tell you who but then I'd have to enter the Witness Protection Program). The Inspecting Organization will want me to cite a range for HUMIDITY in the lab. Because this not only hinges on our weather (we're in Monsoon Season right now and the lab humidity is 47% as I write but is normally between 17-20%), but also MUST be based on any of the histology equipment that may be affected by humidity in any way. Realizing that we all work in different "environments" and usually have little or no control over the HVAC system, what did you decide upon as your HUMIDITY RANGE for the SOP under which you operate? If there are no specific equipment requirements, I will be able to give a range of 0-100% without the necessity for a Corrective Action Report. If I am too specific and give too narrow a range, I may end up filling out a Corrective Action Report for days when I'm "out of range". Please give me as much detail as you are able, without being required to join me in hiding. Your input will be rewarded (don't ask). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From Nancy_Schmitt <@t> pa-ucl.com Thu Jul 14 13:27:21 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Jul 14 13:27:26 2011 Subject: [Histonet] P16 Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367911B7@PEITHA.wad.pa-ucl.com> What are folks using for P16? Thanks for your help- Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Anna.Inman <@t> stmarygj.org Thu Jul 14 13:47:07 2011 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Thu Jul 14 13:47:19 2011 Subject: [Histonet] TTF-1 Message-ID: <2925AE271EAAD440AF48FCCEB8002D091C076CFE@smgmail01.smgj.sclhs.net> Is anyone using the SPT24 clone for TTF-1 using the Ultraview platform on the Benchmark XT? Our pathologists have read this clone is supposed to be more sensitive than Ventana's 8G7G3/1 clone Thank you Anna Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From marktarango <@t> gmail.com Thu Jul 14 13:52:10 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jul 14 13:52:14 2011 Subject: [Histonet] TTF-1 In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D091C076CFE@smgmail01.smgj.sclhs.net> References: <2925AE271EAAD440AF48FCCEB8002D091C076CFE@smgmail01.smgj.sclhs.net> Message-ID: My lab uses the SPT24 clone for TTF-1. Let me know if you'd like any protocol info. Mark On Thu, Jul 14, 2011 at 11:47 AM, Inman, Anna wrote: > Is anyone using the SPT24 clone for TTF-1 using the Ultraview platform > on the Benchmark XT? > > Our pathologists have read this clone is supposed to be more sensitive > than Ventana's 8G7G3/1 clone > > Thank you > > Anna > > > > Anna.Inman@stmarygj.org > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mward <@t> wfubmc.edu Thu Jul 14 14:24:09 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Jul 14 14:24:26 2011 Subject: [Histonet] RE: Lab humidity range In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF896@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B051DF896@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: We chose 10 - 90% as our range after reading what few instrument manuals even addressed the issue. I can only wish we could get to 17% humidity here in NC. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, July 14, 2011 2:23 PM To: Histonet@lists.utsouthwestern.edu Cc: Hanosh, Timothy J.; Jillson, Gregory Subject: [Histonet] Lab humidity range We're prepping for a Big Inspection (I could tell you who but then I'd have to enter the Witness Protection Program). The Inspecting Organization will want me to cite a range for HUMIDITY in the lab. Because this not only hinges on our weather (we're in Monsoon Season right now and the lab humidity is 47% as I write but is normally between 17-20%), but also MUST be based on any of the histology equipment that may be affected by humidity in any way. Realizing that we all work in different "environments" and usually have little or no control over the HVAC system, what did you decide upon as your HUMIDITY RANGE for the SOP under which you operate? If there are no specific equipment requirements, I will be able to give a range of 0-100% without the necessity for a Corrective Action Report. If I am too specific and give too narrow a range, I may end up filling out a Corrective Action Report for days when I'm "out of range". Please give me as much detail as you are able, without being required to join me in hiding. Your input will be rewarded (don't ask). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Jul 14 14:34:37 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jul 14 14:34:41 2011 Subject: [Histonet] Humidididity Ranges Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF8A3@nmdamailsvr.nmda.ad.nmsu.edu> I love your replies because it gives me quite a range in order to avoid Incarceration, but I need to know THE CRITERIA (specific equipment operating parameters, for example?) you used to come to that range. I'm walkin' the plank here and I'm getting close to the end... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From wdesalvo.cac <@t> hotmail.com Thu Jul 14 18:14:28 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Jul 14 18:14:32 2011 Subject: [Histonet] Histobath revised In-Reply-To: <4E1CE3FB.4050702@comcast.net> References: <4E1CE3FB.4050702@comcast.net> Message-ID: We continue to use the Histobath, but the istrument is no longer being produced. I suggest you search the instruent resellers for a unit. We have had to move to the Hacker-Bright Clini RF, a floor model and , of course, the cost is significantly higher. William DeSalvo, B.S., HTL(ASCP) > Date: Tue, 12 Jul 2011 19:16:59 -0500 > From: techonebs@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histobath revised > > Hey again. > > I was not refering to a tissue flotation bath. The Histobath was a > product sold by Shandon. It had a chamber filled with isopentane or > alcohol and was used for quick freezing tissue for a cryostat. Sorry if > there was any confusion. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barbara.Crill <@t> LPNT.net Fri Jul 15 08:01:06 2011 From: Barbara.Crill <@t> LPNT.net (Barbara.Crill@LPNT.net) Date: Fri Jul 15 08:01:15 2011 Subject: [Histonet] Ultra LCS VS regular LCS Message-ID: <7DA79EBDBD92BF408EF392413737878D3943467322@NADCWPMSGCMS01.hca.corpad.net> Is anyone using the LCS for the BenchmarkXT in place of the Ultra LCS in the Ultra instrument? We heard that this could be done with the same results but I am not sure what the difference is between the two LCS's and if you can substitute. Any thought? ANTOINETTE CRILL TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER E-mail: barbara.crill@LPNT.net From edpace96 <@t> yahoo.com Fri Jul 15 09:54:44 2011 From: edpace96 <@t> yahoo.com (Eric Pace) Date: Fri Jul 15 09:54:48 2011 Subject: [Histonet] Job opening in Houston, TX. Message-ID: <1310741684.75952.YahooMailClassic@web80014.mail.sp1.yahoo.com> ANNOUNCEMENT NUMBER: 14600 - T JOB TITLE: Histology Technician II DEPARTMENT: Institute of Forensic Sciences HOURS: 40 Hours Per Week SALARY: Commensurate With Experience Based On 26 Pay Periods EDUCATION: Completion of an Associate?s degree and completion of histology school with histo-technician certification (ASCP) American Society of Clinical Pathologist at the time of employment or within one year of employment. EXPERIENCE: One year of experience in a histology laboratory in which responsibilities included production of stained slides and preservation of tissue samples in both paraffin and formalin. JOB SKILLS: The successful applicant must have expertise in the use of microtomes, manual staining procedures, manual slide coverslipping, automated slide stainers, automated slide coverslipper, tissue processors and tissue embedding. Must be capable of understanding and adhering to strict protocols for the handling, trimming and archival of tissue samples and blocks; knowledge of histology laboratory safety rules and procedures is essential. Good interpersonal skills are a must. JOB DESCRIPTION: Prepares stained slides of autopsy tissues in a careful controlled environment. Prepares paraffin blocks of tissue for long-term storage and labels the samples in accordance with histology laboratory protocols; assists with archival of paraffin-embedded and non-paraffin-embedded formalin fixed tissues. Provides all tissue slides to the assigned Assistant Medical Examiner, Deputy Chief Medical Examiner or Chief Medical Examiner on a timely basis; assists with entry o data into a computerized database to track laboratory efficiency as required. Other job assignments as assigned by the Deputy Chief Medical Examiner. Position requires a high level of confidentiality, responsibility and dependability. PHYSICAL REQUIREMENTS: must be able to sit and stand for prolonged periods of time; able to lift up to 40lbs; stooping and bending may be required. EMPLOYMENT IS CONTINGENT UPON PASSING A CRIMINAL BACKGROUND CHECK. HARRIS COUNTY HAS AN EMPLOYMENT AT WILL POLICY. CLOSING DATE: Open Until Filled APPLY AT: 1310 PRAIRIE - SUITE 170 UPON RECEIVING A CONDITIONAL OFFER OF EMPLOYMENT, ALL APPLICANTS ARE SCREENED FOR THE PRESENCE OF ILLEGAL DRUGS. If you have any question feel free to e-mail back. Thanks, Eric Pace, BS, HT(ASCP) From rjbuesa <@t> yahoo.com Fri Jul 15 10:08:39 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 15 10:08:46 2011 Subject: [Histonet] Lab humidity range Message-ID: <1310742519.75404.YahooMailClassic@web65711.mail.ac4.yahoo.com> The only instruments that could be affected by HIGH humidity are those with electronic relays that could get "humid" and fail. This is something that is taken care off by the A/C systems in any laboratory so I do not think that any usual instrument?will be?affected by humidity. Another thing will be anhydrous chemicals which will require heat dehydration if humidified. As to the range you have 2 choices: ask your maintenance departments the range they set to their A/C - dehumidifying systems, or ask your local meteorological station to give you the historic humidity ranges for your area. Ren? J. --- On Thu, 7/14/11, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Lab humidity range To: Histonet@lists.utsouthwestern.edu Cc: "Hanosh, Timothy J." , "Jillson, Gregory" Date: Thursday, July 14, 2011, 2:23 PM We're prepping for a Big Inspection (I could tell you who but then I'd have to enter the Witness Protection Program).? The Inspecting Organization will want me to cite a range for HUMIDITY in the lab. Because this not only hinges on our weather (we're in Monsoon Season right now and the lab humidity is 47% as I write but is normally between 17-20%), but also MUST be based on any of the histology equipment that may be affected by humidity in any way.???Realizing that we all work in different "environments" and usually have little or no control over the HVAC system, what did you decide upon as your HUMIDITY RANGE for the SOP under which you operate?? If there are no specific equipment requirements, I will be able to give a range of 0-100% without the necessity for a Corrective Action Report.? If I am too specific and give too narrow a range, I may end up filling out a Corrective Action Report for days when I'm "out of range".? Please give me as much detail as you are able, without being required to join me in hiding.? Your input will be rewarded (don't ask). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Fri Jul 15 10:44:36 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Fri Jul 15 11:38:24 2011 Subject: [Histonet] RE: P16 In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C367911B7@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C367911B7@PEITHA.wad.pa-ucl.com> Message-ID: The only company currently allowed to sell it (have it, etc.) MTM Labs 1-866-686-5227 CAT#: 9581 Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, July 14, 2011 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P16 What are folks using for P16? Thanks for your help- Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aeaton <@t> cellmarque.com Fri Jul 15 10:53:22 2011 From: aeaton <@t> cellmarque.com (Adrianna Eaton) Date: Fri Jul 15 11:48:11 2011 Subject: [Histonet] And now, IHC playing cards! In-Reply-To: References: <8CE0FA8D804645B-2794-3F7C@webmail-d180.sysops.aol.com> Message-ID: <0C5591F288E3994DBB023203F7730D241EBCFCAB@cmrocmb2.cellmarque.local> Hello all, The playing cards are still available! We have a cancer quiz on our Cell Marque Facebook (http://www.facebook.com/cellmarque) wall that people can take and if they send us their score and a ship to address, we send them a free deck of cards. If you can?t get on our Facebook page due to firewall restrictions, here?s a direct link to the quiz: http://www.sporcle.com/games/jkgordo/cancersubtypes. E-mail your quiz score and shipping address to marketing@cellmarque.com and you will get your free deck of cards. Thank you for your participation! Best Regards, Adrianna Eaton Technical Consultant 6600 Sierra College Blvd. Rocklin, CA 95677 (916) 746-8900 x8923 (916) 746-8989 (fax) www.cellmarque.com From Passion to Product to Patient.? Learn with Cell Marque on:? ?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, July 14, 2011 7:05 AM To: mtitford@aol.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] And now, IHC playing cards! Hello again! I went to their site and can't find anything...hopefully I didn't miss it!! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, wrote: > > > Those of you that like freebies (like Dako mousepads, Thermo pens and > Sigma coffee mugs) will be amused to learn that Cell Marque are giving > away packs of playing cards. Each card shows a section stained with a > different antobody (one of theirs of course, together with the catalogue number). > > No, I don't have shares in Cell Marque, but I am continually amused by > the different methods companies in all walks of life use to advertise > their products. Some are, well, just original! > > Michael Titford > Pathology USA > Mobile AL USA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Fri Jul 15 12:25:11 2011 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Fri Jul 15 17:19:36 2011 Subject: [Histonet] Lab Humidity Range Message-ID: <2B2ECF33934F5D4996D8BE03EFDF3976085A55B5@VHAV09MSGA3.v09.med.va.gov> Per industrial hygiene: our recycler should be operating at RH 10-15% Any suggestions for our lab would be helpful: we are averaging 58% to 78%. In addition to the HVAC system (new air handler) we run 2 dehumidifiers and a portable air conditioner in our lab to maintain the current humidity levels. We use Formula 83 xylene sub and with quite a lot difficulty from April to September. At our highest you could watch the 100% ETOH change in front of our eyes, thus going thru a lot of reagent. We use H2Blu in the F83 to help absorb any moisture carry over in the stain line and change/rotate F83/100%ETOH frequently, sometimes several times a day. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From ASelf <@t> georgetownhospitalsystem.org Fri Jul 15 13:00:11 2011 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Fri Jul 15 17:34:39 2011 Subject: [Histonet] Meditech Downtime Message-ID: Happy Friday Histonetters, For those of you on Meditech Pathology Module or any other pathology computer system what procedure do you follow for down time? Do you have a time frame that you go by for the amount of time that the computer system will be down for before you start doing everything manually? Hope everyone has a great weekend. Thanks in advance for your help, Amy . NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From FUNKM <@t> mercyhealth.com Fri Jul 15 13:24:43 2011 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Fri Jul 15 17:37:26 2011 Subject: [Histonet] Lab Humidity Range In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF3976085A55B5@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF3976085A55B5@VHAV09MSGA3.v09.med.va.gov> Message-ID: <4E203F9B.E948.00AC.1@mercyhealth.com> Hello- we have major air bubbles with our Leica cover slipper this past week. It been hot and humid in Iowa do other folks have problems ? Do you change your medium for summer ? Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 >>> "White, Lisa M." 7/15/2011 12:25 PM >>> Per industrial hygiene: our recycler should be operating at RH 10-15% Any suggestions for our lab would be helpful: we are averaging 58% to 78%. In addition to the HVAC system (new air handler) we run 2 dehumidifiers and a portable air conditioner in our lab to maintain the current humidity levels. We use Formula 83 xylene sub and with quite a lot difficulty from April to September. At our highest you could watch the 100% ETOH change in front of our eyes, thus going thru a lot of reagent. We use H2Blu in the F83 to help absorb any moisture carry over in the stain line and change/rotate F83/100%ETOH frequently, sometimes several times a day. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Fri Jul 15 13:40:54 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Jul 15 17:43:02 2011 Subject: [Histonet] RE: Meditech Downtime In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA261E076FBB@NADCWPMSGCMS03.hca.corpad.net> Amy, Happy Friday to you!!! We normally stamp our requisitions with a number and when accessioning we tell Meditech what the number is rather than Meditech assigning a number to the case. So, when there is a down time, we continue stamping the requisitions, labeling the specimens, making the cassettes and grossing in. After Meditech comes back up, we accession all the specimens at once. Hope this helps. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Friday, July 15, 2011 2:00 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Meditech Downtime Happy Friday Histonetters, For those of you on Meditech Pathology Module or any other pathology computer system what procedure do you follow for down time? Do you have a time frame that you go by for the amount of time that the computer system will be down for before you start doing everything manually? Hope everyone has a great weekend. Thanks in advance for your help, Amy . NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sarah_taba <@t> yahoo.com Fri Jul 15 14:31:03 2011 From: sarah_taba <@t> yahoo.com (sarah Tabatabaei) Date: Fri Jul 15 17:49:34 2011 Subject: [Histonet] reducing auto-flourescence in human peripheral tissue In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA261E076FBB@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA261E076FBB@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <1310758263.46928.YahooMailNeo@web45713.mail.sp1.yahoo.com> Hi everyone, Does anyone know how to reduce auto-florescence when staining human peripheral tissue with fluorescent IHC? I am trying to stain my human intervertebral discs with PGP 9.5 1:1000. But I always get a huge signal to noise when looking at my samples under the fluorescent microscope. Thank you for your time ? Zahra From rsrichmond <@t> gmail.com Fri Jul 15 19:37:16 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Jul 15 23:51:14 2011 Subject: [Histonet] Re: Histobath revised Message-ID: Would people using the Histobath or its successors please try out the non-flammable fluoroether in place of the potentially explosive acetone or 2-methylbutane? http://www.histosearch.com/histonet/May08A/RE.HistonetRe.Alternative.html Bob Richmond Samurai Pathologist Knoxville TN From ruppert.amysue <@t> marshfieldclinic.org Fri Jul 15 22:10:57 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Sat Jul 16 00:13:41 2011 Subject: [Histonet] autoclave water Message-ID: <201107160310.p6G3At87016070@mailhost2.mfldclin.edu> Do these jars go through your lab dishwashing dept? Could it be soap residue or some other residue from the dishwashing? Have you tried to rinse the jars well prior to putting the water for autoclaving? The dishwashing dept should be able to test for any residue. amysue ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From kiran_g <@t> sbcglobal.net Sat Jul 16 19:33:47 2011 From: kiran_g <@t> sbcglobal.net (kiran_g@sbcglobal.net) Date: Sat Jul 16 19:33:54 2011 Subject: [Histonet] IHC Validation Message-ID: <1741709272-1310862828-cardhu_decombobulator_blackberry.rim.net-2051366286-@b4.c14.bise6.blackberry> How long it will take to do validation if you have to start with 20 stains ? Any tips to make it more efficient will be appreciated. Thank you -K Sent from my Verizon Wireless BlackBerry From olstrauss <@t> gmail.com Sun Jul 17 01:01:05 2011 From: olstrauss <@t> gmail.com (Otto Strauss) Date: Sun Jul 17 01:01:32 2011 Subject: [Histonet] Light microscopy to get orientation for fluorescent Message-ID: <1A3F0615-3578-4C66-BA31-D2E40E60753E@gmail.com> I am trying to do fluorescent immunohistochemistry on liver tissue. Unfortunately because the liver is such a homogenous tissue it is difficult to get an appropriate orientation with just fluoroscopic views. I was wondering if there is a way of staying with non fluorescent dyes(like H&E) so that I can get a picture with light microscopy, to have orientation within the liver when viewing it with fluorescent microscopy? Regards. Otto Auckland NZ From dr_sadushe <@t> yahoo.com Sun Jul 17 08:12:30 2011 From: dr_sadushe <@t> yahoo.com (Sadushe Loxha) Date: Sun Jul 17 08:12:35 2011 Subject: [Histonet] (no subject) Message-ID: <1310908350.55904.YahooMailMobile@web36104.mail.mud.yahoo.com> http://www.killahmodels.com/blog/wp-content/themes/tubally.htm From anonwums1 <@t> gmail.com Sun Jul 17 09:51:49 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Sun Jul 17 09:54:56 2011 Subject: [Histonet] Light microscopy to get orientation for fluorescent In-Reply-To: <1A3F0615-3578-4C66-BA31-D2E40E60753E@gmail.com> References: <1A3F0615-3578-4C66-BA31-D2E40E60753E@gmail.com> Message-ID: Try differential interference microscopy (DIC). Many fluorescent scopes will have this as an option. It will give you faint outlines of the cells. Adam On Sun, Jul 17, 2011 at 1:01 AM, Otto Strauss wrote: > I am trying to do fluorescent immunohistochemistry on liver tissue. > Unfortunately because the liver is such a homogenous tissue it is difficult > to get an appropriate orientation with just fluoroscopic views. > > I was wondering if there is a way of staying with non fluorescent dyes(like > H&E) so that I can get a picture with light microscopy, to have orientation > within the liver when viewing it with fluorescent microscopy? > > Regards. > > Otto > Auckland > NZ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Sun Jul 17 09:54:31 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jul 17 09:56:52 2011 Subject: [Histonet] IHC Validation In-Reply-To: <1741709272-1310862828-cardhu_decombobulator_blackberry.rim.net-2051366286-@b4.c14.bise6.blackberry> Message-ID: <1310914471.92271.YahooMailClassic@web65705.mail.ac4.yahoo.com> It will depend in how you do the validation: manually or with a high output autostainer. Ren? J. --- On Sat, 7/16/11, kiran_g@sbcglobal.net wrote: From: kiran_g@sbcglobal.net Subject: [Histonet] IHC Validation To: histonet@lists.utsouthwestern.edu Date: Saturday, July 16, 2011, 8:33 PM How long it will take to do validation if you have to start with 20 stains ? Any tips to make it more efficient will be appreciated. Thank you -K Sent from my Verizon Wireless BlackBerry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Sun Jul 17 17:10:33 2011 From: member <@t> linkedin.com (Sheree Holmes via LinkedIn) Date: Sun Jul 17 17:10:36 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1217399822.5174163.1310940633806.JavaMail.app@ela4-bed81.prod> LinkedIn ------------ Sheree Holmes requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Sheree Accept invitation from Sheree Holmes http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I133508382_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYOe3cUc3kPcP59bT9NtPlhgCBAbPgTc3cNdPcSdjcLrCBxbOYWrSlI/EML_comm_afe/ View invitation from Sheree Holmes http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I133508382_13/3cNnP8UcPwMdjcPckALqnpPbOYWrSlI/svi/ ------------------------------------------ DID YOU KNOW your LinkedIn profile helps you control your public image when people search for you? Setting your profile as public means your LinkedIn profile will come up when people enter your name in leading search engines. Take control of your image! http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/ewp/inv-22/ -- (c) 2011, LinkedIn Corporation From epeters2 <@t> gmu.edu Sun Jul 17 18:10:36 2011 From: epeters2 <@t> gmu.edu (Esther Peters) Date: Sun Jul 17 18:10:39 2011 Subject: [Histonet] Water droplets on slides Message-ID: <4E236BEC.90509@gmu.edu> I am having problems with water droplets appearing on slides when transferring the rack from the last 100% dehydrant (Richard-Allan) to either SafeClear II or xylenes. I saw that some of you had noted this problem might be in the xylene substitute, but switching to xylene seemed to solve it. I am wondering if the "100%" isn't? I have poured immediately before use from freshly opened bottles, and still have the problem. Would appreciate any help (of course, the humidity is high right now, but...)! -- Esther C. Peters, Ph.D., Assistant Professor; Department of Environmental Science & Policy; George Mason University From megan.french <@t> mcri.edu.au Sun Jul 17 22:43:25 2011 From: megan.french <@t> mcri.edu.au (Megan French) Date: Sun Jul 17 22:45:01 2011 Subject: [Histonet] RNAlater buffer & fixation/embedding in OCT Message-ID: Has anyone successful fixed & embedded tissues in OCT after using RNAlater buffer? I've found a paper that says this doesn't affect tissue integrity but I need for info regarding protocol (To defrost? Fixative? Timing? Temperature Etc) ...and more than anything feedback from someone who has used/uses this product. Product: http://www.ambion.com/techlib/resources/RNAlater/ Paper: http://www.nature.com/modpathol/journal/v14/n2/pdf/3880267a.pdf Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne) Research Assistant Surgical Research; Infection & Immunity Theme Murdoch Children's Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia E megan.french@mcri.edu.au * ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From megan.french <@t> mcri.edu.au Sun Jul 17 22:44:55 2011 From: megan.french <@t> mcri.edu.au (Megan French) Date: Sun Jul 17 22:45:05 2011 Subject: [Histonet] positive staining on my secondary only controls...need a DAB superquench? Message-ID: Having some trouble with my IHC optimisation. Hopefully it's a simple fix someone may be able to provide some guidance (Im a junior RA! Hello histoworld!). Im using a DAB protocol with swine-anti-rabbit HRP on human colon. I have successful staining of my primary (yay!), somewhat successful staining with my antibody preabsorbed with peptide (yet still some staining)...BUT im seeing positive staining on my secondary only controls (may explain why im getting a bit of staining on my preabsorped specimens). I quench with 5% hydrogen peroxide for 5 min on day 1 (prior to primaries/no primary if secondary control) and on day 2 add 1 DAKO DAB chromogen tablet to 10ml PBS and 10ul hydrogen peroxide again before immersing slides for 8 min, then stain with haematoxylin per usual, dehydrate, clear & mount. I feel like I need a super quencher or something?! Any tips would be most helpful. (My slides also undergo antigen retrieval in citrate buffer ph6 for 10min @ 95deg and 20min at RT) Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne) Research Assistant Surgical Research; Infection & Immunity Theme Murdoch Children's Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia E megan.french@mcri.edu.au * ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From lori.w <@t> sympatico.ca Mon Jul 18 05:27:00 2011 From: lori.w <@t> sympatico.ca (L White) Date: Mon Jul 18 05:27:03 2011 Subject: [Histonet] Lab humidity range In-Reply-To: References: Message-ID: Sakura has developed a threshold for humidity if you are using their film coverslipper. Other than that, I am not aware of any specific requirements for routine histology equipment. Lori From Nancy_Schmitt <@t> pa-ucl.com Mon Jul 18 08:24:28 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Jul 18 08:26:32 2011 Subject: [Histonet] P16 on the BOND Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36791319@PEITHA.wad.pa-ucl.com> Happy Monday- I should have been more specific in my question - is anyone using p16 on the BOND IHC instrument? Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From TJJ <@t> stowers.org Mon Jul 18 08:44:24 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Jul 18 08:46:29 2011 Subject: [Histonet] Re: Histobath revised Message-ID: <2C40E43D1F7A56408C4463FD245DDDF977056669@EXCHMB-02.stowers-institute.org> Dr. Richmond recommended using non-flammable and non-explosive Novec (tm) Engineered Fluid HFE-7100. We are currently using this for our freezing and it works very well. The main thing we have noticed is the blocks float in it and do not sink as they would with other solvents. It is a 3M product, and my contact there last year was ebinder@mmm.com (Erin Binder). Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From leticia.figliuolo <@t> roche.com Mon Jul 18 08:44:39 2011 From: leticia.figliuolo <@t> roche.com (Figliuolo, Leticia) Date: Mon Jul 18 08:46:31 2011 Subject: [Histonet] Alcian blue-safranin Message-ID: <069E6CF048B915488720412DAFD1474D03FC377975@RNUMSEM702.nala.roche.com> Hello everybody, I was wondering if anyone has a protocol for Alcian Blue-Safranin (for mast cells). I would greatly appreciate any help. Thank you! Leticia Figliuolo e-mail: leticia.figliuolo@roche.com From Ronald.Houston <@t> nationwidechildrens.org Mon Jul 18 08:57:38 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Jul 18 08:57:52 2011 Subject: [Histonet] RE: Re: Histobath revised In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF977056669@EXCHMB-02.stowers-institute.org> References: <2C40E43D1F7A56408C4463FD245DDDF977056669@EXCHMB-02.stowers-institute.org> Message-ID: I second Terri's comments Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Monday, July 18, 2011 9:44 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Histobath revised Dr. Richmond recommended using non-flammable and non-explosive Novec (tm) Engineered Fluid HFE-7100. We are currently using this for our freezing and it works very well. The main thing we have noticed is the blocks float in it and do not sink as they would with other solvents. It is a 3M product, and my contact there last year was ebinder@mmm.com (Erin Binder). Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From liz <@t> premierlab.com Mon Jul 18 09:32:44 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Jul 18 09:35:33 2011 Subject: [Histonet] positive staining on my secondary only controls...need a DAB superquench? In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA051A@SBS2K8.premierlab.local> Megan Pre absorbing your antibody for a control for IHC is not always going to work there are publications out there that do not recommend it. I'll try to find the reference I'm talking about. I pulled this out of one of my presentations Protocols available on the web - for western blots http://www.upstate.com/misc/protocol_detail.q.prot.e.peptide-competion.a.name.e.Peptide_Competition http://store.crpinc.com/prot_peptide_comp.aspx "The absorption control is less important because if cannot determine whether the protein bound in the tissue is the same protein that used for absorption. Therefore, the recommended guidelines for immunohistochemistry include negative controls and positive controls but do not include absorption controls." Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Megan French Sent: Sunday, July 17, 2011 9:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] positive staining on my secondary only controls...need a DAB superquench? Having some trouble with my IHC optimisation. Hopefully it's a simple fix someone may be able to provide some guidance (Im a junior RA! Hello histoworld!). Im using a DAB protocol with swine-anti-rabbit HRP on human colon. I have successful staining of my primary (yay!), somewhat successful staining with my antibody preabsorbed with peptide (yet still some staining)...BUT im seeing positive staining on my secondary only controls (may explain why im getting a bit of staining on my preabsorped specimens). I quench with 5% hydrogen peroxide for 5 min on day 1 (prior to primaries/no primary if secondary control) and on day 2 add 1 DAKO DAB chromogen tablet to 10ml PBS and 10ul hydrogen peroxide again before immersing slides for 8 min, then stain with haematoxylin per usual, dehydrate, clear & mount. I feel like I need a super quencher or something?! Any tips would be most helpful. (My slides also undergo antigen retrieval in citrate buffer ph6 for 10min @ 95deg and 20min at RT) Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne) Research Assistant Surgical Research; Infection & Immunity Theme Murdoch Children's Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia E megan.french@mcri.edu.au * ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Mon Jul 18 10:02:37 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Mon Jul 18 10:02:46 2011 Subject: [Histonet] RE: P16 on the BOND In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36791319@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36791319@PEITHA.wad.pa-ucl.com> Message-ID: Hi Nancy, I am using the p16 with the Bond. I am using the Bond's ER1 for 20min with their Refine detection kit. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Monday, July 18, 2011 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P16 on the BOND Happy Monday- I should have been more specific in my question - is anyone using p16 on the BOND IHC instrument? Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV <@t> mail.amc.edu Mon Jul 18 10:16:57 2011 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Mon Jul 18 10:19:49 2011 Subject: [Histonet] Spirabrush Message-ID: Hi, I was wondering if anyone has had any experience with processing specimens obtained using the Spirabrush for GYN cases. We have an account who would like to submit his specimens using this method and we wanted to know if people are handling these as Cytology specimens or Histology specimens. Any information would be greatly appreciated. Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From sbreeden <@t> nmda.nmsu.edu Mon Jul 18 11:05:18 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Jul 18 11:05:23 2011 Subject: [Histonet] Leica SELECTECH Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF8C1@nmdamailsvr.nmda.ad.nmsu.edu> Need to speak with a Technical contact regarding SelecTech staining system - someone who can give details about expiration dates. Cannot find contact info online. Thanks! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From liz <@t> premierlab.com Mon Jul 18 11:14:23 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Jul 18 11:14:26 2011 Subject: [Histonet] acceptance criteria of IHC stains Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0524@SBS2K8.premierlab.local> Hello everyone Has anyone out there had to write acceptance criteria for a USP document, any help would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 From lpwenk <@t> sbcglobal.net Mon Jul 18 11:43:56 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jul 18 11:44:06 2011 Subject: [Histonet] Peggy Wenk's job postings Message-ID: <033A63EE883E4A36AFBE246D19F2A0DB@HP2010> Histonetters - There seems to be a little glitch in our hospital's application process. Several people have said that they have applied for the position of Program Director for the Schools of Histotechnology at William Beaumont Hospital, Royal Oak, MI. But no one is showing up in the system. Could all those interested please go two things: 1. Reapply 2. Send resume (in a separate email, in addition to attaching it to the application process) to our Human Resources person and to me, just in case the application doesn't show up again. Diane Soper, Human Resources dsoper@beaumont.edu Peggy Wenk, School, pwenk@beaumont.edu For more information about the job, and directions on where to apply, go to the NSH website: www.nsh.org On the right, click on Career Center On the left/middle, click on View Jobs Look for the 6/30/11 Program Director position, click on View I apologize for the inconvenience. Peggy A. Wenk, HTL(ASCP)SLS Program Director Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 Lee & Peggy Wenk From dbpiontek <@t> hotmail.com Mon Jul 18 12:37:38 2011 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Mon Jul 18 12:37:45 2011 Subject: [Histonet] Re: Message-ID: There?s nothing better to have some fun in your bed! ... http://nacar-ar.com.ar/inbox.php?ohotmailID=37fi5 From christina.thurby <@t> bms.com Mon Jul 18 14:35:32 2011 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Mon Jul 18 14:35:47 2011 Subject: [Histonet] Paraffin Block Storage & Temperature Range Message-ID: Hello, Can some of you all let me know the set ranges for paraffin block storage rooms. We are currently storing our paraffin blocks in a controlled temperature room, but may need to increase the temperature high point to 81 F and the critical high point to 86 F. Does this sound appropriate? We are using Paraplast, the vendor product information says to store at temperatures below 97 F. I am curious to know at what temperatures the blocks will begin 'sticking together'. Thanks! Christina Thurby Bristol Myers Squibb 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From abright <@t> brightinstruments.com Mon Jul 18 15:14:21 2011 From: abright <@t> brightinstruments.com (abright@brightinstruments.com) Date: Mon Jul 18 15:14:33 2011 Subject: [Histonet] Re: Histobath revised In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF977056669@EXCHMB-02.stowers-institute.org> References: <2C40E43D1F7A56408C4463FD245DDDF977056669@EXCHMB-02.stowers-institute.org> Message-ID: <766564020-1311020062-cardhu_decombobulator_blackberry.rim.net-1480678361-@b26.c2.bise7.blackberry> We supply a container with our Clini-Rapid freezer that stops the specimen (blocks) floating in the Novec solution. Alan Bright www.brightinstruments.com Sent from my BlackBerry? wireless device -----Original Message----- From: "Johnson, Teri" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 18 Jul 2011 08:44:24 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Histobath revised Dr. Richmond recommended using non-flammable and non-explosive Novec (tm) Engineered Fluid HFE-7100. We are currently using this for our freezing and it works very well. The main thing we have noticed is the blocks float in it and do not sink as they would with other solvents. It is a 3M product, and my contact there last year was ebinder@mmm.com (Erin Binder). Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01F9pM6D8) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01F9pM6D8&m=d2b41b86c463&t=20110718&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01F9pM6D8&m=d2b41b86c463&t=20110718&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01F9pM6D8&m=d2b41b86c463&t=20110718&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From Keri.Colwell <@t> inspection.gc.ca Mon Jul 18 16:21:05 2011 From: Keri.Colwell <@t> inspection.gc.ca (Keri Colwell) Date: Mon Jul 18 16:21:19 2011 Subject: [Histonet] Microtome draft shield Message-ID: <4E244F60.C0AD.00BF.1@inspection.gc.ca> Hi Everyone, I work in a lab which is under negative pressure (air is continuously flowing in), and due to the layout of the rooms are microtomes are located next to two different doorways. We are looking for some sort of draft shield to place around each microtome and water bath that will reduce the effects of the airflow and personnel movements on our ribbons. Anyone have any suggestions as to who might sell such a thing? Thanks in advance! Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone: 403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada From drmoses111 <@t> comcast.net Mon Jul 18 16:51:20 2011 From: drmoses111 <@t> comcast.net (drmoses111@comcast.net) Date: Mon Jul 18 16:51:23 2011 Subject: [Histonet] Thymoma control block to share Message-ID: <1738069675.782437.1311025880102.JavaMail.root@sz0037a.westchester.pa.mail.comcast.net> Hello, I am working up TdT and I need a formalin fixed paraffin embedded thymoma block. The thymus from my autopies are just not optimal. I would gladly share any of control blocks in trade. Thank you, Renee Boston-Moses HT,QIHC Histology Department Virtua Health System 100 Bowman Dr. Voorhees, NJ 08043 From abright <@t> brightinstruments.com Tue Jul 19 04:53:09 2011 From: abright <@t> brightinstruments.com (abright@brightinstruments.com) Date: Tue Jul 19 04:53:29 2011 Subject: [Histonet] Microtome draft shield In-Reply-To: <4E244F60.C0AD.00BF.1@inspection.gc.ca> References: <4E244F60.C0AD.00BF.1@inspection.gc.ca> Message-ID: <165730107-1311069194-cardhu_decombobulator_blackberry.rim.net-602771580-@b26.c2.bise7.blackberry> Dear Keri, Our Bright M3500 microtome is fitted with a clear retractable visor over the specimen and knife area that will more than likely solve your problem. Please take a look on www.brightinstruments.com Alan Bright Bright Instrument Co. Cambs England Sent from my BlackBerry? wireless device -----Original Message----- From: "Keri Colwell" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 18 Jul 2011 17:21:05 To: Subject: [Histonet] Microtome draft shield Hi Everyone, I work in a lab which is under negative pressure (air is continuously flowing in), and due to the layout of the rooms are microtomes are located next to two different doorways. We are looking for some sort of draft shield to place around each microtome and water bath that will reduce the effects of the airflow and personnel movements on our ribbons. Anyone have any suggestions as to who might sell such a thing? Thanks in advance! Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone: 403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01F9xp2Mf) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01F9xp2Mf&m=d96cad56b2e4&t=20110718&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01F9xp2Mf&m=d96cad56b2e4&t=20110718&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01F9xp2Mf&m=d96cad56b2e4&t=20110718&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From trathborne <@t> somerset-healthcare.com Tue Jul 19 08:11:06 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jul 19 08:14:20 2011 Subject: [Histonet] Microtome draft shield In-Reply-To: <4E244F60.C0AD.00BF.1@inspection.gc.ca> References: <4E244F60.C0AD.00BF.1@inspection.gc.ca> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707030FFC@SMCMAIL01.somerset-healthcare.com> Haven't seen anything that specific, but you might want to look at the various biohazard splash guards. They are a clear Plexiglas, and they have a base to support them. You would be able to move them around (or have them mounted to the counter if desired), and they come in an assortment of angles and sizes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Monday, July 18, 2011 5:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome draft shield Hi Everyone, I work in a lab which is under negative pressure (air is continuously flowing in), and due to the layout of the rooms are microtomes are located next to two different doorways. We are looking for some sort of draft shield to place around each microtome and water bath that will reduce the effects of the airflow and personnel movements on our ribbons. Anyone have any suggestions as to who might sell such a thing? Thanks in advance! Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone: 403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From jclark <@t> pcnm.com Tue Jul 19 10:11:10 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Tue Jul 19 10:11:50 2011 Subject: [Histonet] ER/PR Message-ID: <0494A7D4E8CC254EA2FB81464982E3786E8884@S10MAILD001N1.SH10.lan> Hi All, I would like some input on ER/PR by IHC. We have a lot of problems with tissue detachment from the slides. We use a DAKO autostainer and do the heat retrieval in the Pascal pressure cooker. We use high pH retrieval buffer and heat to 120 degrees and hold for 1 minute, but when you look microscopically at the slides the heat has really played a number on the tissue and it looks chewed up. The slides have been in the slide dryer for at least an hour before running, sometimes longer. Any ideas how I can reduce the effects of the heat on the tissue without compromising the staining? Thanks for your help. Joanne Clark, HT Histology Supervisor PCNM From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Jul 19 10:20:54 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Jul 19 10:23:07 2011 Subject: [Histonet] RE: ER/PR In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3786E8884@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3786E8884@S10MAILD001N1.SH10.lan> Message-ID: We heat ours to 99C for 20 minutes with a cool down. Using the Dako PT link. (water bath) BUT....before you change your pretreatment Make sure that your tissue is fixed properly, processed properly. After it is cut, make sure that the slides dry before you baked them in the oven. Any water left under those sections will lift the tissue off for sure. Hope that helps. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark [jclark@pcnm.com] Sent: Tuesday, July 19, 2011 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR Hi All, I would like some input on ER/PR by IHC. We have a lot of problems with tissue detachment from the slides. We use a DAKO autostainer and do the heat retrieval in the Pascal pressure cooker. We use high pH retrieval buffer and heat to 120 degrees and hold for 1 minute, but when you look microscopically at the slides the heat has really played a number on the tissue and it looks chewed up. The slides have been in the slide dryer for at least an hour before running, sometimes longer. Any ideas how I can reduce the effects of the heat on the tissue without compromising the staining? Thanks for your help. Joanne Clark, HT Histology Supervisor PCNM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WBRID <@t> capefearvalley.com Tue Jul 19 13:09:41 2011 From: WBRID <@t> capefearvalley.com (Wendy Bridges) Date: Tue Jul 19 13:09:46 2011 Subject: [Histonet] HSV I&II control slides Message-ID: Does anyone out there have any HSV I&II tissues available for controls? Newcomer does not supply them anymore. Thank you, Wendy Bridges, HT (ASCP), LTS Department of Pathology Cape Fear Valley Health System Fayetteville, NC 910-615-6142 CONFIDENTIALITY NOTICE: This electronic mail transmission may contain information that is privileged and/or confidential. Additionally, this communication may contain individual protected health information ("PHI") that is subject to protection under state and federal laws, or other privileged, confidential or proprietary information of Cape Fear Valley Health System that may not be further disclosed. Please be advised that any disclosure, copying, distribution or other use of the contents of this message by anyone other than the intended recipient is prohibited. If you have received this communication in error, please notify the sender immediately by replying to the message and deleting it from your computer. From mward <@t> wfubmc.edu Tue Jul 19 13:24:34 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Jul 19 13:24:47 2011 Subject: [Histonet] C FLIP AND NKF Beta antibodies Message-ID: I have been asked about a research project involving these two antibodies and we are having some trouble finding sources that are appropriate for formalin fixed, paraffin embedded tissues. Does anyone have experience with these antibodies? Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From a.byrnes <@t> accelpath.com Tue Jul 19 13:38:53 2011 From: a.byrnes <@t> accelpath.com (Andrew Byrnes) Date: Tue Jul 19 13:39:01 2011 Subject: [Histonet] locums pathologist survey Message-ID: <81B464DB-4316-4CF1-B7A4-8D57E0CB6BC9@accelpath.com> Dear Histonet, I was wondering if anyone in histoland can tell me what your experience has been with a locums pathologist. Cost? And how was the service? My understanding is that they can cost around $2500 per day PLUS expenses. Thanks in advance, Andrew Andrew Byrnes www.AccelPath.com From mbrooks <@t> incytepathology.com Tue Jul 19 13:50:02 2011 From: mbrooks <@t> incytepathology.com (Matt Brooks) Date: Tue Jul 19 13:50:10 2011 Subject: [Histonet] Evening Histotech Position Opening Message-ID: <706224670091FE47997AEF88EFADE7CA01FA56AA@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello All, I have a full time evening position available for a registered HT or HTL in Spokane Valley, WA. The hours are 5:00 p.m. to 1:30 a.m. M-F. InCyte Pathology is a great company; it was voted one of the top 100 Washington Best Companies to work for List in 2011. Check out the website at http://incytepathology.com/. We offer an excellent benefits package including medical, dental, vision, annual bonus, 401K and profit sharing. Let me know if you are interested. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 (W) 720-253-7204 (C) From ASelf <@t> georgetownhospitalsystem.org Tue Jul 19 14:12:06 2011 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue Jul 19 14:12:14 2011 Subject: [Histonet] Assisting with Autopsies Message-ID: Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From trathborne <@t> somerset-healthcare.com Tue Jul 19 14:14:48 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jul 19 14:16:23 2011 Subject: [Histonet] RE: Assisting with Autopsies In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB757070311E6@SMCMAIL01.somerset-healthcare.com> Although our autopsy volume is also low, our two PA's take turns assisting. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From ChaseM <@t> childrensdayton.org Tue Jul 19 14:18:26 2011 From: ChaseM <@t> childrensdayton.org (Matthew Chase) Date: Tue Jul 19 14:18:33 2011 Subject: [Histonet] RE: Assisting with Autopsies In-Reply-To: References: Message-ID: <9719621676F71F49AEA7CCC6F4561B3D045A99548F@PEXCHNG1.cmc-dayton.org> We are small too, about 3-5 per year and that is one thing I hate...yes we do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: The information contained in this e-mail and any accompanying documents or files is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited and possibly a violation of federal/state law or regulations. If you received this information in error, please notify The Children's Medical Center of Dayton immediately via telephone at (937) 641-5293, or via electronic mail cmcconfidentiality@childrensdayton.org and promptly destroy the original message. Thank you. From LSetlak <@t> childrensmemorial.org Tue Jul 19 14:18:27 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Tue Jul 19 14:18:41 2011 Subject: [Histonet] RE: Assisting with Autopsies In-Reply-To: References: Message-ID: <7111DB39D045004C9CF29E79C71B28BC10882F4235@CMHEXCC01MBX.childrensmemorial.org> We have a diener and residents who help and our PA as well. We do about 40 a year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 2:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From diane.gladney <@t> us.army.mil Tue Jul 19 14:24:12 2011 From: diane.gladney <@t> us.army.mil (Gladney, Diane C Ms CIV USA MEDCOM MACH) Date: Tue Jul 19 14:24:36 2011 Subject: [Histonet] RE: Assisting with Autopsies (UNCLASSIFIED) In-Reply-To: References: Message-ID: Classification: UNCLASSIFIED Caveats: NONE Many years ago when we did autopsies here, the Histotechs helped. We were more like a pair of extra hands but that can be most helpful to the pathologist. We are a military hospital and some of the active duty Medical Techs liked to come and help us out. We closed our autopsy suite in the mid 1990's since we were performing only 4-6 autopsies per year. If we have need for an autopsy, they are performed at the VA hospital here. They are a large hospital with their own denier. All in all, it was a great experience but not something that I really miss doing. Thanks, Diane G. Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a denier to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE From trathborne <@t> somerset-healthcare.com Tue Jul 19 14:27:53 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jul 19 14:38:50 2011 Subject: [Histonet] Controls with patient specimen on same slide Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Bauer.Karen <@t> mayo.edu Tue Jul 19 14:41:23 2011 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Jul 19 14:41:30 2011 Subject: [Histonet] Assisting with Autopsies In-Reply-To: References: Message-ID: <53FC421CC200C5429929EDE6C3676F3001807ED5@msgebe34> Hi Amy, We do about the same number of autopsies per year... Only on in-house patients and they have to be physician requested, no family requested. We used to hire a diener to come and do them and the techs would assist. None of the techs cared to do that, so I usually volunteered, since I actually like doing them. Eventually, the docs decided to just train me and now I'm the hospital diener. It was a lot easier to have a full time employee around to do them than try to have an outsider come in once in a while. Easier to keep up on competencies, vacation times and such. Before, if the diener was on vacation, there was no one else to do them. The docs were not happy when they had to do it themselves. :) Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.karen@mayo.edu ___________________________________________ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 2:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Tue Jul 19 14:42:31 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jul 19 14:42:37 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> Message-ID: Hi Toni, We put the control on the same slide and after it's stained and coverslipped we draw a line to seperate the control and patient tissue with each side of the line having either a "C" or "P" written on it. Mark On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni < trathborne@somerset-healthcare.com> wrote: > > Hi, > I'm interested in knowing how many of you are performing ihc with the > control tissue and the patient tissue on the same slide. I have seen slides > available which have designated areas for each tissue to be placed so there > will not be any confusion. If you're doing it, have you encountered any > problems? What benefits have you noticed since implementing this process? > Are your pathologists in favor of this? If you're not, why not? > Thanks, > Toni > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LSebree <@t> uwhealth.org Tue Jul 19 14:46:25 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jul 19 14:46:30 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> Message-ID: Whenever possible we put patient tissue on slides with positive control tissue on it. Everyone likes it and then you're assured your control and patient got the same staining experience. We used to use the "red box" control slides but discontinued due to the additional cost. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, July 19, 2011 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls with patient specimen on same slide Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Tue Jul 19 14:56:56 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Tue Jul 19 14:57:06 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> Message-ID: <7111DB39D045004C9CF29E79C71B28BC10882F4239@CMHEXCC01MBX.childrensmemorial.org> We often do the same or use separate slides. We used to use the slides with the designated "control" area but had some problems with the reagents getting to all areas of the slide. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Tuesday, July 19, 2011 2:43 PM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Controls with patient specimen on same slide Hi Toni, We put the control on the same slide and after it's stained and coverslipped we draw a line to seperate the control and patient tissue with each side of the line having either a "C" or "P" written on it. Mark On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni < trathborne@somerset-healthcare.com> wrote: > > Hi, > I'm interested in knowing how many of you are performing ihc with the > control tissue and the patient tissue on the same slide. I have seen slides > available which have designated areas for each tissue to be placed so there > will not be any confusion. If you're doing it, have you encountered any > problems? What benefits have you noticed since implementing this process? > Are your pathologists in favor of this? If you're not, why not? > Thanks, > Toni > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue Jul 19 15:20:07 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Jul 19 15:20:16 2011 Subject: [Histonet] RE: Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> Message-ID: Toni, I've been doing this for over a decade with great success. As far as I'm concerned, there is no better way to ensure that your IHC slide worked. When you use a batch control slide for CD20 in position #3, that is not proof that something didn't go wrong during the process on the CD20 in position #9. The only time that I use batch controls is when I receive precut slides for staining with no room for control tissue. The biggest drawback is trying to fit both a known positive control section and the patient tissue on the same slide when the patient's section is very large. Another problem is that some IHC's are very particular in wanting a FRESH control section which makes it difficult to have precut control slides (if it is only ordered occasionally). I am a reference IHC lab & all the pathologists that I do work for like the practice. Glen Dawson BS, HT (ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, July 19, 2011 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls with patient specimen on same slide Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Jul 19 15:22:49 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jul 19 15:22:53 2011 Subject: [Histonet] RE: Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082122A62B@CHEXCMS10.one.ads.che.org> We do that. Not only does it save the cost of an extra slide, it assures that both sections are treated the exact same way. Our pathologists are very happy with it. We don't use the expensive slides, we just always put the control at the top. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, July 19, 2011 15:28 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls with patient specimen on same slide Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From azdudley <@t> hotmail.com Tue Jul 19 15:43:56 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue Jul 19 15:44:00 2011 Subject: [Histonet] er pr validation Message-ID: I know this has been talked about before here but how many slides are people doing for er pr validation, and are you doing it with another hosp? thanks so much for your help. anita dudley providence hosp mobile al From marktarango <@t> gmail.com Tue Jul 19 15:55:52 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jul 19 15:55:55 2011 Subject: [Histonet] er pr validation In-Reply-To: References: Message-ID: Hi Anita, We ran 40 cases and sent the same blocks to Phenopath for them to run for comparision. There is a requistion for doing an ER/PR validation on their website. Mark On Tue, Jul 19, 2011 at 1:43 PM, anita dudley wrote: > > I know this has been talked about before here but how many slides are > people doing for er pr validation, and are you doing it with another hosp? > thanks so much for your help. > > anita dudley > providence hosp > mobile al > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Tue Jul 19 17:03:04 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Jul 19 17:03:12 2011 Subject: [Histonet] Light microscopy to get orientation for fluorescent Message-ID: Hi Otto, It is common to use DAPI as basically a fluorescent counter stain for this. It labels nucleii in a blue band with so it shouldn't interfere with other (red green) labeling. Vector makes a fluorescent mounting media that contains DAPI and it is really simple to just put the coverslip on right after you finish and it's done. Amos Message: 2 Date: Sun, 17 Jul 2011 18:01:05 +1200 From: Otto Strauss Subject: [Histonet] Light microscopy to get orientation for fluorescent To: "histonet@lists.utsouthwestern.edu" Message-ID: <1A3F0615-3578-4C66-BA31-D2E40E60753E@gmail.com> Content-Type: text/plain; charset=us-ascii I am trying to do fluorescent immunohistochemistry on liver tissue. Unfortunately because the liver is such a homogenous tissue it is difficult to get an appropriate orientation with just fluoroscopic views. I was wondering if there is a way of staying with non fluorescent dyes(like H&E) so that I can get a picture with light microscopy, to have orientation within the liver when viewing it with fluorescent microscopy? Regards. Otto Auckland NZ From dreynold <@t> mdanderson.org Tue Jul 19 17:06:10 2011 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Tue Jul 19 17:06:19 2011 Subject: [Histonet] Re: ER/PR In-Reply-To: References: Message-ID: <785BBF0C5F49CE41BA74460A43A08F022EA0189F60@DCPWVMBXC0VS3.mdanderson.edu> I don't work with these antibodies but when we were doing bone marrow bx pressure cooker, high pH retrieval we lost a lot of tissue. This was what we had previously work out for our antibody. We replaced the pressure cooker with a 70?C water bath, kept the high pH retrieval and cooked for 2 hours. The stain and the morphology were beautiful. We lost none of our tissue. We also tried 4 hours with the same conditions but decided the 2 hours best for our tissue and antibody. ------------------------------ Message: 9 Date: Tue, 19 Jul 2011 11:20:54 -0400 From: "McMahon, Loralee A" Subject: [Histonet] RE: ER/PR To: Joanne Clark , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We heat ours to 99C for 20 minutes with a cool down. Using the Dako PT link. (water bath) BUT....before you change your pretreatment Make sure that your tissue is fixed properly, processed properly. After it is cut, make sure that the slides dry before you baked them in the oven. Any water left under those sections will lift the tissue off for sure. Hope that helps. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark [jclark@pcnm.com] Sent: Tuesday, July 19, 2011 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR Hi All, I would like some input on ER/PR by IHC. We have a lot of problems with tissue detachment from the slides. We use a DAKO autostainer and do the heat retrieval in the Pascal pressure cooker. We use high pH retrieval buffer and heat to 120 degrees and hold for 1 minute, but when you look microscopically at the slides the heat has really played a number on the tissue and it looks chewed up. The slides have been in the slide dryer for at least an hour before running, sometimes longer. Any ideas how I can reduce the effects of the heat on the tissue without compromising the staining? Thanks for your help. Joanne Clark, HT Histology Supervisor PCNM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 24 **************************************** From Rcartun <@t> harthosp.org Tue Jul 19 17:40:26 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jul 19 17:40:35 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> Message-ID: <4E25CF9A.7400.0077.1@harthosp.org> We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 7/19/2011 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Jul 19 18:01:27 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jul 19 18:01:37 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <4E25CF9A.7400.0077.1@harthosp.org> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> <4E25CF9A.7400.0077.1@harthosp.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082122A647@CHEXCMS10.one.ads.che.org> We used to do batch controls too. And we cut unstained slides in a similar fashion also. In order to put the control on the already cut slide, one must stand on one's head or hang themselves upside down from the rafters, but it can be done.. Just put the slide in the water label end first and you have it! Took me years to get it going, and took awhile for techs to adjust, but now it's very routine. And if you use a derm punch you can get lots of control tissue from your blocks without destroying the block. And you save many dollars and give the pathologists assurance that the tissue was treated the same. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, July 19, 2011 18:40 To: histonet@lists.utsouthwestern.edu; Toni Rathborne Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 7/19/2011 >>> 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rsrichmond <@t> gmail.com Tue Jul 19 18:20:52 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Jul 19 18:21:01 2011 Subject: [Histonet] Re: locum tenens pathologist survey Message-ID: Andrew Byrnes at www.AccelPath.com asks: >>I was wondering if anyone in histoland can tell me what your experience has been with a locums pathologist. Cost? And how was the service? My understanding is that they can cost around $2500 per day PLUS expenses.<< Well, having been in locum tenens pathology practice for over 30 years, more than 60 pathology services - now 72 years old and still at it. First of all, it's "locum tenens". There is no such word as "locums". You hire a locum tenens pathologist. The Latin literally means "holding a place" (for someone who's not there). The plural is locos tenentes, sounds like the crazy people who rent upstairs. I've never been paid more than $700 a day, sometimes with mileage and motel room paid for. I have the idea that the locum tenens agency charges about the same amount again, so that you pay twice as much to an agency as you'd pay me. Sort of like flat-backin'. I would encourage anyone wanting to hire a locum tenens pathologist to negotiate with them directly, rather than working through an agency, understanding that management types are more comfortable working with agencies where they can talk to their own kind of people. My relationships with my private clients have always been much better than when I had an agency mediating the relationship. Your pathologist should handle this rather than delegating it, using the College of American Pathologists Web site as a resource - though I wish the Web site functioned a lot better than it does. I'll be happy to talk with your pathologist (or with you!) about all of these matters. I hope that I continue to provide exemplary service! Flagrant self-promotion now - I'm licensed in TN NC MO GA KY. Bob Richmond Samurai Pathologist Knoxville TN From tkngflght <@t> yahoo.com Tue Jul 19 20:18:16 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Jul 19 20:18:20 2011 Subject: [Histonet] Temps needed --call if curious? Message-ID: <1311124696.42285.YahooMailClassic@web39423.mail.mud.yahoo.com> ? From tkngflght <@t> yahoo.com Tue Jul 19 20:33:31 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Jul 19 20:33:36 2011 Subject: [Histonet] Temps needed --call if curious? WITH phone number In-Reply-To: <1311124696.42285.YahooMailClassic@web39423.mail.mud.yahoo.com> Message-ID: <1311125611.65337.YahooMailClassic@web39410.mail.mud.yahoo.com> Blank email--sorry!? User glitch. ? Okeydoke--what do you want to know?? Fifteen minutes on the phone will get you the following: ? --the good vs. bad of traveling (yup--kinda like?most other jobs) --how it works (and doesn't work) --who pays for what and what will I make? --what happens when I'm done? --why it might (or might NOT) for for you.... ? Satisfy that curiousity.? We have a couple of positions to?staff and more coming across the next few months.? I won't push you-- I will make sure you have all the information you need to make the right decision for yourself and your family. ? Plus it's a really fun conversation!? Give me a call? ? Cheryl Kerry, HT(ASCP) & long-time travel tech 281.852.9457 Full Staff Inc. ? ? From Laura.Miller <@t> leica-microsystems.com Tue Jul 19 21:10:19 2011 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Tue Jul 19 21:10:28 2011 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 07/19/2011 and will not return until 07/20/2011. I am out of the office. I will return on July 21st. I will reply to your message on Thursday ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From k84as <@t> yahoo.com Tue Jul 19 21:18:03 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Tue Jul 19 21:18:10 2011 Subject: [Histonet] (no subject) Message-ID: <1311128283.70818.YahooMailMobile@web112607.mail.gq1.yahoo.com> http://tricorengineering.com.au/tmp/templates_c/ma1.htm From Susan.Walzer <@t> HCAHealthcare.com Wed Jul 20 02:31:55 2011 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Wed Jul 20 02:32:05 2011 Subject: [Histonet] RE: Assisting with Autopsies In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2BB00D27F2@FWDCWPMSGCMS09.hca.corpad.net> We are a small hospital and though we no longer do in house autopsies we still had an autopsy assistant.(most prefer to be called this) We always had a pool of people available to do this job. Training as a histotech does not include this job and I have always refused to do it. I know there are techs who do not mind and some who supplement their income doing it but histotechs should not be forced to do them, certainly not free of charge. If enough histotechs in an area stand together and refuse the pathologists will find assistants. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Jul 20 05:06:01 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Jul 20 05:06:14 2011 Subject: [Histonet] RE: Assisting with Autopsies In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2BB00D27F2@FWDCWPMSGCMS09.hca.corpad.net> References: <4BF03F5404EBDE409AF9232DA74B9DED2BB00D27F2@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF04EEAE@LRGHEXVS1.practice.lrgh.org> We have always helped with autopsies, just comes with the territory. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, July 20, 2011 3:32 AM To: ASelf@georgetownhospitalsystem.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Assisting with Autopsies We are a small hospital and though we no longer do in house autopsies we still had an autopsy assistant.(most prefer to be called this) We always had a pool of people available to do this job. Training as a histotech does not include this job and I have always refused to do it. I know there are techs who do not mind and some who supplement their income doing it but histotechs should not be forced to do them, certainly not free of charge. If enough histotechs in an area stand together and refuse the pathologists will find assistants. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From TMcNemar <@t> lmhealth.org Wed Jul 20 05:13:00 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jul 20 05:13:11 2011 Subject: [Histonet] RE: Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> Message-ID: We put patients and controls on the same slide. We used to use the slides with the red box for the control but encountered staining issues. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, July 19, 2011 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls with patient specimen on same slide Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From histotech <@t> imagesbyhopper.com Wed Jul 20 05:18:41 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Jul 20 05:18:59 2011 Subject: [Histonet] RE: Assisting with Autopsies In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DF04EEAE@LRGHEXVS1.practice.lrgh.org> References: <4BF03F5404EBDE409AF9232DA74B9DED2BB00D27F2@FWDCWPMSGCMS09.hca.corpad.net> <38667E7FB77ECD4E91BFAEB8D986386323DF04EEAE@LRGHEXVS1.practice.lrgh.org> Message-ID: <75816322-DA7C-44DE-A342-38B6691671E7@imagesbyhopper.com> We do, on average, about 15 autopsies per year. We have a diener who does them for us. The pathologists are present and are often are hands on during the autopsy. Sometimes a lab aide will be there in the mode of a scribe. Our histotechs do not participate in autopsies. Michelle On Jul 20, 2011, at 6:06 AM, "Podawiltz, Thomas" wrote: > We have always helped with autopsies, just comes with the territory. > > > > Tom Podawiltz HT (ASCP) > Histology Section Head/Laboratory Safety Officer. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com > Sent: Wednesday, July 20, 2011 3:32 AM > To: ASelf@georgetownhospitalsystem.org; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Assisting with Autopsies > > We are a small hospital and though we no longer do in house autopsies we still had an autopsy assistant.(most prefer to be called this) We always had a pool of people available to do this job. Training as a histotech does not include this job and I have always refused to do it. I know there are techs who do not mind and some who supplement their income doing it but histotechs should not be forced to do them, certainly not free of charge. If enough histotechs in an area stand together and refuse the pathologists will find assistants. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self > Sent: Tuesday, July 19, 2011 3:12 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Assisting with Autopsies > > Hello All, > > We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. > > Does your facility use histotechs or a diener to assist with the autopsy? > > > Thanks in advance for all of your help, Amy > > > Amy Self > Georgetown Hospital System > 843-527-7179 > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lisa.White3 <@t> va.gov Wed Jul 20 06:47:49 2011 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Wed Jul 20 06:48:01 2011 Subject: [Histonet] Assisting with Autopsies Message-ID: <2B2ECF33934F5D4996D8BE03EFDF3976085F3F5A@VHAV09MSGA3.v09.med.va.gov> Here we all take turns doing the autopsies. We have call for weekends/holidays as well. We do have pathologist and residents in the suite with us, however we do the harvest and hand everything in one block from the trachea to the testicles to the docs to dissect and then we remove the brain and pituitary and place those if fixative for them. Clean up the body (put it back into the crypt) and then clean as much of the suite as possible while the docs are still dissecting the block. It is hard work and with the type of patient that we service it is not uncommon to have difficulties with extensive adhesions etc. I have had patients so large that I could hardly look into the cavity where I was trying to cut (needed a ladder). Years ago at a community hospital I was only an extra pair of hands, ahhh the good old days. You will need to be trained and it doesn't sound like your facility do enough to do this quickly, if they insist that HT/HTL be diener to assist then they need to have a good sign off procedure to train you properly so that there are no safety issues and give you time to practice. The other thing for us is we basically loose one tech for the day by the time they are finished and if you are like most labs and short staffed this can be an issue in getting the daily workload out on time. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax Date: Tue, 19 Jul 2011 15:12:06 -0400 From: Amy Self Subject: [Histonet] Assisting with Autopsies To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From DKBoyd <@t> chs.net Wed Jul 20 07:28:07 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Jul 20 07:28:19 2011 Subject: [Histonet] Assisting with Autopsies In-Reply-To: Message-ID: We have two histotechs who assist with autopsies. It is part of their job description. We also have an 800 number for an independent company that performs autopsies on site or at a local mortuary. These are usually cases that are suspicious for litigation by the family. We only perform autopsies on "in house" patients and at the attending physician's request. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Amy Self Sent by: histonet-bounces@lists.utsouthwestern.edu 07/19/2011 03:12 PM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From trathborne <@t> somerset-healthcare.com Wed Jul 20 08:18:52 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jul 20 08:20:33 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <4E25CF9A.7400.0077.1@harthosp.org> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> <4E25CF9A.7400.0077.1@harthosp.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 7/19/2011 >>> 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From thiggins <@t> cddmedical.com Wed Jul 20 08:27:02 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Wed Jul 20 08:27:06 2011 Subject: [Histonet] Controls with patient specimen on same slide References: <20110719230426.C84CB10CCDD3@barracuda.crvinc.net> Message-ID: <000f01cc46e0$b6b5fb20$e001a8c0@cdd.loc> It is the most effective and economical way to run your IHC's. We have been doing it that way for as long as I have been in histology. Thanks, Tim ----- Original Message ----- From: To: Sent: Tuesday, July 19, 2011 6:04 PM Subject: Histonet Digest, Vol 92, Issue 25 Hi, > I'm interested in knowing how many of you are performing ihc with the > control tissue and the patient tissue on the same slide. I have seen slides > available which have designated areas for each tissue to be placed so there > will not be any confusion. If you're doing it, have you encountered any > problems? What benefits have you noticed since implementing this process? > Are your pathologists in favor of this? If you're not, why not? > Thanks, > Toni From LSebree <@t> uwhealth.org Wed Jul 20 08:52:32 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Jul 20 08:53:33 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com><4E25CF9A.7400.0077.1@harthosp.org> <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> Message-ID: Just one. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 8:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 7/19/2011 >>> 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Kuhnla <@t> chsli.org Wed Jul 20 09:45:59 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Jul 20 09:46:30 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com><4E25CF9A.7400.0077.1@harthosp.org> <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> Message-ID: Each slide or else the whole theory of knowing each slide got treated the same goes out the window. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 7/19/2011 >>> 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From settembr <@t> umdnj.edu Wed Jul 20 09:48:54 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Wed Jul 20 09:49:10 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> <4E25CF9A.7400.0077.1@harthosp.org> <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> Message-ID: If I have a cytokeratin AE1/AE3 to run on 5 blocks of the same patient, I use only 1 positive control slide (same slide as patient tissue) Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 7/19/2011 >>> 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Jul 20 10:02:22 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jul 20 10:02:32 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> <4E25CF9A.7400.0077.1@harthosp.org> <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> Message-ID: We do put controls on each slide in a case. Sometimes it's just one slide that failed in a run. A batch control wouldn't tell you which slide failed if there are no internal controls in the patient tissue. I personally wouldn't feel comfortable doing IHC with batch controls. Mark On Wed, Jul 20, 2011 at 6:18 AM, Rathborne, Toni < trathborne@somerset-healthcare.com> wrote: > All of these responses are great. So here's a follow up question. > Do you place a control tissue on EACH slide if you have multiple blocks for > a case, or just on one of the slides? > > -----Original Message----- > From: Richard Cartun [mailto:Rcartun@harthosp.org] > Sent: Tuesday, July 19, 2011 6:40 PM > To: histonet@lists.utsouthwestern.edu; Rathborne, Toni > Subject: Re: [Histonet] Controls with patient specimen on same slide > > We do not put our positive control tissue on the test slide; we run batch > controls. Many of the unstained slides (breast, GI, and prostate biopsies) > that we use for IHC testing are cut in our Histology Laboratory as part of a > part-type slide protocol. For example, we cut 7 slides, 2 sections on each > slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if > needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for > us to place the positive control tissue on the same slide. In addition, I > receive a lot of consult cases from other hospitals where they send us > unstained slides for testing. Once again, it would be difficult to place > the positive control tissue on the same slide and it would slow us down in > terms of starting those slides once they arrive. However, I think the main > reason we don't pursue putting the positive control tissue on the same slide > is the fact that it would consume an enormous amount of control tissue. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Rathborne, Toni" 7/19/2011 > >>> 3:27 PM >>> > > Hi, > I'm interested in knowing how many of you are performing ihc with the > control tissue and the patient tissue on the same slide. I have seen slides > available which have designated areas for each tissue to be placed so there > will not be any confusion. If you're doing it, have you encountered any > problems? What benefits have you noticed since implementing this process? > Are your pathologists in favor of this? If you're not, why not? > Thanks, > Toni > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error by > e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, > ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------------------------------------------------- > This message was secured by ZixCorp(R). > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amkmadden <@t> gmail.com Wed Jul 20 10:32:07 2011 From: amkmadden <@t> gmail.com (Amanda Madden) Date: Wed Jul 20 10:32:31 2011 Subject: [Histonet] Leica Service Technician Message-ID: Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda From SHargrove <@t> urhcs.org Wed Jul 20 10:35:33 2011 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Wed Jul 20 10:35:39 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: Message-ID: In our Lab we put a control on every slide. We keep multiple controls cut and stored in refrigerator. Most are multi- tissue controls. We use the Ventana detection kits and it is not cost efficient to use 2 uses of the detection kit per antibody. Of course there are times that this will not work, then our cost per test goes up, but we cannot charge any more for a control. (Embedded image moved to file: pic32000.jpg) From Robert.Cordero <@t> comphealth.com Wed Jul 20 10:37:44 2011 From: Robert.Cordero <@t> comphealth.com (Robert Cordero) Date: Wed Jul 20 10:37:51 2011 Subject: [Histonet] please remove me from the list, thank you! Message-ID: <0BA36F96367E8A4CBB27E112FAA6488A0679E9@vslcexmbp02.mychg.com> From MSHERWOOD <@t> PARTNERS.ORG Wed Jul 20 10:43:46 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jul 20 10:43:54 2011 Subject: [Histonet] Leica Service Technician In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB58D3@PHSXMB30.partners.org> Amanda, We have a Microm HM550 cryostat and have used Brian J. Hurley (New England Biomedical Services). He is an independent service engineer. He's very good. In fact ThermoFisher uses him if a service visit is needed. 781-331-8642 617-774-7368 (cell) Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Ronald.Houston <@t> nationwidechildrens.org Wed Jul 20 10:55:50 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Jul 20 10:56:04 2011 Subject: [Histonet] Cat Scratch control Message-ID: Does anyone have an extra Cat Scratch control block to spare? We currently buy control sections from Newcomer, but I'm not convinced they are that good. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From TMcNemar <@t> lmhealth.org Wed Jul 20 11:26:11 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jul 20 11:26:19 2011 Subject: [Histonet] Leica Service Technician In-Reply-To: References: Message-ID: It is really just a freezer with a microtome in it. Do you have an on-site refrigeration guy that could look at it? I have used our in-house guy a time or two. Ours is pretty simple and does not have all of the electronics that you may have on the Leica but it is worth a shot. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From TMcNemar <@t> lmhealth.org Wed Jul 20 11:30:06 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jul 20 11:30:14 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570703120D@SMCMAIL01.somerset-healthcare.com> <4E25CF9A.7400.0077.1@harthosp.org> <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> Message-ID: Each case gets one positive control per antibody. If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 7/19/2011 >>> 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From JWeems <@t> sjha.org Wed Jul 20 11:53:21 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jul 20 11:53:25 2011 Subject: [Histonet] RE: Assisting with Autopsies In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082122A73D@CHEXCMS10.one.ads.che.org> We have two contract deiners.. They work at other hospitals around town and charge per case. Works well here as our numbers have decreased drastically over the years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 15:12 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Nancy_Schmitt <@t> pa-ucl.com Wed Jul 20 11:59:53 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Jul 20 12:00:01 2011 Subject: [Histonet] Controls with patient specimen on same slide In-Reply-To: <20110720150625.D7CCA1A5739@mail.pa-ucl.com> References: <20110720150625.D7CCA1A5739@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36791511@PEITHA.wad.pa-ucl.com> We place control tissue on EACH slide for IHC testing - even if multiple blocks from same case. Nancy Schmitt HT, MLT(ASCP) United Clinical Laboratories ----------------------------------------- Message: 12 Date: Wed, 20 Jul 2011 13:18:52 +0000 From: "Rathborne, Toni" Subject: RE: [Histonet] Controls with patient specimen on same slide To: "'Richard Cartun'" , "histonet@lists.utsouthwestern.edu" Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From HornHV <@t> archildrens.org Wed Jul 20 12:17:48 2011 From: HornHV <@t> archildrens.org (Horn,Hazel) Date: Wed Jul 20 12:17:59 2011 Subject: [Histonet] RE: Assisting with Autopsies In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB7198B85ACEA@EVS1.archildrens.org> We have an autopsy assistant and when he's not busy with autopsies (which is often) he works in our histology lab as an assistant!! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 2:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From nicole <@t> dlcjax.com Wed Jul 20 13:08:10 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Jul 20 13:08:24 2011 Subject: [Histonet] Leica Service Technician In-Reply-To: References: Message-ID: <3934.208.62.167.196.1311185290.squirrel@webmail.realpages.com> Amanda, Im not exactly sure which part of your machine is freezing based on your description, but, I have a leica 1510 and it has freezing issues. Everyone I have ever used does this. The bar that hold the specimen chucks ices and freezes over like crazy.. It will frezze the chuck in the bar holder solid. I have to get a hammer and beat it sometimes..lol. So, before I use my machine I wipe 100% alcohol across the bar to de-ice it and the chucks dont get stuck..Do not use so much that you lower your temp. Also, do not get on the oct or the stage because your blocks will not cut and be mush... Just wipe the areas daily with alcohol.. Hope this helps. Nicole Tatum, HT ASCP Hi Histonetters! > > A few months back I emailed regarding a service contract through Leica for > our CM 3050S, and (unfortunately?) we chose not to purchase one. This week > we have had a serious issue with it... the specimen head and arm is > covered > in frost, and the object temperature sensor is reading ## instead of a > temp. > In any case, we called Leica and asked for a service call, but it is > extremely expensive and they couldn't give us an estimate of when they > will > be here because cryostats used for clinical applications have priority > over > those, like ours, that are used for research. Understandable, but > frustrating nonetheless. So my question is: does anyone know of a good, > reputable cryostat service technician (who is authorized by leica, if > possible) that is located in the Boston, MA area? > > Any help would be greatly appreciated! > > Thanks in advance, > Amanda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From donna_suresch <@t> merck.com Wed Jul 20 13:16:40 2011 From: donna_suresch <@t> merck.com (Suresch, Donna L.) Date: Wed Jul 20 13:16:52 2011 Subject: [Histonet] RE: Leica Service Technician In-Reply-To: References: Message-ID: <2C9A1D9608959940943F357E0A470FF89765A7FB5A@USCTMXP51005.merck.com> Hello Amanda, We are located in Eastern Pa and we use Belair Instruments out of NJ (908-518-2662). We have been very happy with their service on our cryostats. Donna L. Suresch Merck Research Laboratories Research Biologist Imaging Research - West Point -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 20, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 92, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Leica Service Technician (Amanda Madden) 2. Controls with patient specimen on same slide (SHargrove@urhcs.org) 3. please remove me from the list, thank you! (Robert Cordero) 4. RE: Leica Service Technician (Sherwood, Margaret ) 5. Cat Scratch control (Houston, Ronald) 6. RE: Leica Service Technician (Tom McNemar) 7. RE: Controls with patient specimen on same slide (Tom McNemar) 8. RE: Assisting with Autopsies (Weems, Joyce) 9. Controls with patient specimen on same slide (Nancy Schmitt) ---------------------------------------------------------------------- Message: 1 Date: Wed, 20 Jul 2011 11:32:07 -0400 From: Amanda Madden Subject: [Histonet] Leica Service Technician To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda ------------------------------ Message: 2 Date: Wed, 20 Jul 2011 10:35:33 -0500 From: SHargrove@urhcs.org Subject: [Histonet] Controls with patient specimen on same slide To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" In our Lab we put a control on every slide. We keep multiple controls cut and stored in refrigerator. Most are multi- tissue controls. We use the Ventana detection kits and it is not cost efficient to use 2 uses of the detection kit per antibody. Of course there are times that this will not work, then our cost per test goes up, but we cannot charge any more for a control. (Embedded image moved to file: pic32000.jpg) ------------------------------ Message: 3 Date: Wed, 20 Jul 2011 15:37:44 +0000 From: Robert Cordero Subject: [Histonet] please remove me from the list, thank you! To: "Histonet@lists.utsouthwestern.edu" Message-ID: <0BA36F96367E8A4CBB27E112FAA6488A0679E9@vslcexmbp02.mychg.com> Content-Type: text/plain; charset="us-ascii" ------------------------------ Message: 4 Date: Wed, 20 Jul 2011 11:43:46 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] Leica Service Technician To: "Amanda Madden" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB58D3@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" Amanda, We have a Microm HM550 cryostat and have used Brian J. Hurley (New England Biomedical Services). He is an independent service engineer. He's very good. In fact ThermoFisher uses him if a service visit is needed. 781-331-8642 617-774-7368 (cell) Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 5 Date: Wed, 20 Jul 2011 15:55:50 +0000 From: "Houston, Ronald" Subject: [Histonet] Cat Scratch control To: "'histonet@lists.utsouthwestern.edu'" , "ihcrg@googlegroups.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have an extra Cat Scratch control block to spare? We currently buy control sections from Newcomer, but I'm not convinced they are that good. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 6 Date: Wed, 20 Jul 2011 12:26:11 -0400 From: Tom McNemar Subject: RE: [Histonet] Leica Service Technician To: 'Amanda Madden' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" It is really just a freezer with a microtome in it. Do you have an on-site refrigeration guy that could look at it? I have used our in-house guy a time or two. Ours is pretty simple and does not have all of the electronics that you may have on the Leica but it is worth a shot. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 7 Date: Wed, 20 Jul 2011 12:30:06 -0400 From: Tom McNemar Subject: RE: [Histonet] Controls with patient specimen on same slide To: "'Rathborne, Toni'" , 'Richard Cartun' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Each case gets one positive control per antibody. If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 7/19/2011 >>> 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 8 Date: Wed, 20 Jul 2011 12:53:21 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Assisting with Autopsies To: Amy Self , "'histonet@lists.utsouthwestern.edu'" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082122A73D@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We have two contract deiners.. They work at other hospitals around town and charge per case. Works well here as our numbers have decreased drastically over the years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 15:12 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 9 Date: Wed, 20 Jul 2011 16:59:53 +0000 From: Nancy Schmitt Subject: [Histonet] Controls with patient specimen on same slide To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36791511@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" We place control tissue on EACH slide for IHC testing - even if multiple blocks from same case. Nancy Schmitt HT, MLT(ASCP) United Clinical Laboratories ----------------------------------------- Message: 12 Date: Wed, 20 Jul 2011 13:18:52 +0000 From: "Rathborne, Toni" Subject: RE: [Histonet] Controls with patient specimen on same slide To: "'Richard Cartun'" , "histonet@lists.utsouthwestern.edu" Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 27 **************************************** Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rennie1108 <@t> yahoo.com Wed Jul 20 14:48:14 2011 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Wed Jul 20 14:48:18 2011 Subject: [Histonet] Research microtomes Message-ID: <1311191294.91032.YahooMailNeo@web59614.mail.ac4.yahoo.com> Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne From ratliffjack <@t> hotmail.com Wed Jul 20 16:02:19 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Jul 20 16:03:02 2011 Subject: [Histonet] Research microtomes In-Reply-To: <1311191294.91032.YahooMailNeo@web59614.mail.ac4.yahoo.com> References: <1311191294.91032.YahooMailNeo@web59614.mail.ac4.yahoo.com> Message-ID: What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: > Hello Histo-land, > > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rennie1108 <@t> yahoo.com Wed Jul 20 16:57:48 2011 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Wed Jul 20 16:57:56 2011 Subject: [Histonet] Research microtomes In-Reply-To: References: <1311191294.91032.YahooMailNeo@web59614.mail.ac4.yahoo.com> Message-ID: <1311199068.60287.YahooMailNeo@web59611.mail.ac4.yahoo.com> Hi Jack, We're trying to cut just plain old FFPE blocks. ________________________________ From: Jack Ratliff To: Adrienne Anderson Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: > Hello Histo-land, > > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ratliffjack <@t> hotmail.com Wed Jul 20 17:13:43 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Jul 20 17:14:01 2011 Subject: [Histonet] Research microtomes In-Reply-To: <1311199068.60287.YahooMailNeo@web59611.mail.ac4.yahoo.com> References: <1311191294.91032.YahooMailNeo@web59614.mail.ac4.yahoo.com> <1311199068.60287.YahooMailNeo@web59611.mail.ac4.yahoo.com> Message-ID: Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson wrote: > Hi Jack, > > We're trying to cut just plain old FFPE blocks. > > From: Jack Ratliff > To: Adrienne Anderson > Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" > Sent: Wednesday, July 20, 2011 5:02 PM > Subject: Re: [Histonet] Research microtomes > > What type of specimen are you trying to cut? What embedding media are you using? > > Jack > > > > On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: > > > Hello Histo-land, > > > > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! > > > > Thanks, > > Adrienne > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From rsrichmond <@t> gmail.com Wed Jul 20 17:45:19 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Jul 20 17:45:23 2011 Subject: [Histonet] embalmers using formaldehyde - NYT story Message-ID: This New York Times story about the use of formaldehyde by embalmers is worth reading by pathologists and histotechnologists. http://www.nytimes.com/2011/07/21/business/despite-cancer-risk-embalmers-stay-with-formaldehyde.html?_r=1&hp Seems like they've basically got the right idea. Bob Richmond Samurai Pathologist Knoxville TN From saby_joseph_a <@t> yahoo.com Wed Jul 20 17:45:47 2011 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Wed Jul 20 17:45:50 2011 Subject: [Histonet] Microtome draft shield In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707030FFC@SMCMAIL01.somerset-healthcare.com> References: <4E244F60.C0AD.00BF.1@inspection.gc.ca> <3AD061FE740D464FAC7BF6B5CFB75707030FFC@SMCMAIL01.somerset-healthcare.com> Message-ID: <1311201947.45523.YahooMailNeo@web114401.mail.gq1.yahoo.com> I would suggest that you look inthe local yellow pages for people who work with plexiglass.? This is a re;atively inexpensive medium.? You can design what you need and have your draft shields built to order. ? Joe Saby NAMSA From: "Rathborne, Toni" To: 'Keri Colwell' ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, July 19, 2011 9:11 AM Subject: RE: [Histonet] Microtome draft shield Haven't seen anything that specific, but you might want to look at the various biohazard splash guards. They are a clear Plexiglas, and they have a base to support them. You would be able to move them around (or have them mounted to the counter if desired), and they come in an assortment of angles and sizes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Monday, July 18, 2011 5:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome draft shield Hi Everyone, I work in a lab which is under negative pressure (air is continuously flowing in), and due to the layout of the rooms are microtomes are located next to two different doorways.? We are looking for some sort of draft shield to place around each microtome and water bath that will reduce the effects of the airflow and personnel movements on our ribbons. Anyone have any suggestions as to who might sell such a thing? Thanks in advance! Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640? | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone:? 403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbpiontek <@t> hotmail.com Thu Jul 21 08:11:33 2011 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Thu Jul 21 08:11:42 2011 Subject: [Histonet] Re: Message-ID: Sexual problems are to be solved... http://ravera.todouy.com/bestsite.php?opage=20f0 From rennie1108 <@t> yahoo.com Thu Jul 21 09:49:22 2011 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Thu Jul 21 09:49:30 2011 Subject: [Histonet] Research microtomes In-Reply-To: References: <1311191294.91032.YahooMailNeo@web59614.mail.ac4.yahoo.com> <1311199068.60287.YahooMailNeo@web59611.mail.ac4.yahoo.com> Message-ID: <1311259762.22661.YahooMailNeo@web59603.mail.ac4.yahoo.com> This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: "In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns." Thanks, Adrienne ________________________________ From: Jack Ratliff To: Adrienne Anderson Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" Sent: Wednesday, July 20, 2011 6:13 PM Subject: Re: [Histonet] Research microtomes Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson wrote: Hi Jack, > >We're trying to cut just plain old FFPE blocks. > > > >________________________________ >From: Jack Ratliff >To: Adrienne Anderson >Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" >Sent: Wednesday, July 20, 2011 5:02 PM >Subject: Re: [Histonet] Research microtomes > >What type of specimen are you trying to cut? What embedding media are you using? > >Jack > > > >On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: > >> Hello Histo-land, >> >> I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! >> >> Thanks, >> Adrienne >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > From ratliffjack <@t> hotmail.com Thu Jul 21 10:07:09 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jul 21 10:07:30 2011 Subject: [Histonet] Research microtomes In-Reply-To: <1311259762.22661.YahooMailNeo@web59603.mail.ac4.yahoo.com> References: <1311191294.91032.YahooMailNeo@web59614.mail.ac4.yahoo.com> <1311199068.60287.YahooMailNeo@web59611.mail.ac4.yahoo.com> <1311259762.22661.YahooMailNeo@web59603.mail.ac4.yahoo.com> Message-ID: Thanks a lot for your response Adrienne! I think I may be able to help you with this. Is there a time we can talk off-line over the phone? My number is 317-281-1975. Best Regards, Jack On Jul 21, 2011, at 9:49 AM, Adrienne Anderson wrote: > This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: > > "In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. > > Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns." > > Thanks, > Adrienne > > From: Jack Ratliff > To: Adrienne Anderson > Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" > Sent: Wednesday, July 20, 2011 6:13 PM > Subject: Re: [Histonet] Research microtomes > > Couple more questions. :) What is the tissue and the dimensions of the specimen? > > > > On Jul 20, 2011, at 4:57 PM, Adrienne Anderson wrote: > >> Hi Jack, >> >> We're trying to cut just plain old FFPE blocks. >> >> From: Jack Ratliff >> To: Adrienne Anderson >> Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" >> Sent: Wednesday, July 20, 2011 5:02 PM >> Subject: Re: [Histonet] Research microtomes >> >> What type of specimen are you trying to cut? What embedding media are you using? >> >> Jack >> >> >> >> On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: >> >> > Hello Histo-land, >> > >> > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! >> > >> > Thanks, >> > Adrienne >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >> > > From micropathlabs <@t> yahoo.com Thu Jul 21 10:34:10 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Jul 21 10:34:13 2011 Subject: [Histonet] Small Slide Scanner Message-ID: <1311262450.88288.YahooMailRC@web161715.mail.bf1.yahoo.com> Hi all. Is anyone out there using one of the small, low volume slide scanners for use in?frozen sections? We have?looked at a couple of the larger scanners for other purposes but?wanted to know how the smaller ones would?work for our pathologists to?review frozen slides from off-site facilities. Of course, a PA would gross?the specimen at the facility,?cut the slides and?then load them into the scanner. Any thoughts? ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From carpj <@t> wadsworth.org Thu Jul 21 11:36:59 2011 From: carpj <@t> wadsworth.org (carpj) Date: Thu Jul 21 11:37:06 2011 Subject: [Histonet] Fast Blue stabilization Message-ID: Hi List, We are retrogradely labeling spinal motoneurons with Fast Blue, which works well. When we process our sections (60-?m) for immunohistochemical labeling, the Fast Blue signal becomes much weaker. We believe that the detergents we are using to facilitate antibody penetration are also allowing the Fast Blue to leak out of the cells. Is anyone aware of methods for stabilizing Fast Blue within the cells so it won't wash out during immunohistochemical processing? Jonathan Carp, Ph.D. Wadsworth Center New York State Dept. Health Albany, NY IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From epeters2 <@t> gmu.edu Thu Jul 21 12:49:45 2011 From: epeters2 <@t> gmu.edu (Esther Peters) Date: Thu Jul 21 12:49:50 2011 Subject: [Histonet] Histotechnologist positions at George Washington University Message-ID: <4E2866B9.6000304@gmu.edu> I am posting the following position announcements for Dr. Patricia Latham at GWU, in Washington, DC. If you are interested in the positions, please contact her directly: platham@mfa.gwu.edu. Thank you! One position is funded for development of a histopathology core lab in the GW School of Biomedical Sciences. This lab will be expected to provide tissue processing, sections, routine and special stains and immunostaining to meet the needs of researchers at GWU, primarily in the Biomedical sciences, but all departments will be invited to submit tissues. This person will need to work independently to deliver excellent quality results in a timely manner from the get-go. The person should have good managerial skills, since there will be a need to maintain inventory and to keep track of the flow of specimens and charges. Ideally, the person would have an entreprenurial spirit since the success of the lab will determine its future existence. The position is assured for 2 years, but it could grow to full-time, if the lab does well. The second position is not available quite yet (but very soon) and this person must also be a qualified histotechnologist with experience. This is a contract position at half-time to provide a service to a Pharma project - now scheduled through May 2015. The work involved for the contract will be very limited to a select number of routine stains and one immunostain. However, the person will need to be entering data into an audit-trail database and to maintain meticulous records and interdepartmental communications on a limited but international scale. There will be significant time not involved in the contract work for research projects that I intend to pursue. It would be ideal for someone wanting to take an advanced degree or to get involved in biomedical research. This person will be more like a research assistant, except for the contract obligations. Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy George Mason University Fairfax, VA 22030-4444 From mfisher <@t> ecrmc.org Thu Jul 21 13:30:44 2011 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Thu Jul 21 13:30:52 2011 Subject: [Histonet] RPM on Cytocentrifuge for CSF Message-ID: How long and at what RPM do you process your CSF cytocentrifuge specimens? Thank you. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Jul 21 14:02:17 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Jul 21 14:02:23 2011 Subject: [Histonet] University of Pittsburg Message-ID: Hi Histonet, Can someone from the University of Pittsburg immunohistochemistry department please send me an email. One of the pathologists here wanted me to find out what you all do for Hep C. Do you perform the immuno or other? Thank you in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From tallyguy555 <@t> comcast.net Thu Jul 21 14:48:59 2011 From: tallyguy555 <@t> comcast.net (tallyguy555@comcast.net) Date: Thu Jul 21 14:49:02 2011 Subject: [Histonet] Veterinary Histology Message-ID: <1260161372.91386.1311277739861.JavaMail.root@sz0168a.westchester.pa.mail.comcast.net> Greetings, ? My name is Kevin and I've been working in a pathology?/ clinical lab setting for the past 8 yrs. I've recently heard about veterinary histology and the possibilities seem endless.? My initial searches?have not been very productive and I've even heard that certain states do not allow such studies....?Is this true ?? if so, which ones...? ? I currently live in Tallahassee, Florida and?will be re-locating east ( Jacksonville - Daytona Beach, Fl?) in the near future. ? Can anyone help enlighten me ?? Any and all help would be greatly appreciated. ? God Bless, ? Kevin R. Evelyn KWB Pathology Assoc Tallahassee, Florida From tammy <@t> surgicalpathlabs.com Thu Jul 21 15:30:10 2011 From: tammy <@t> surgicalpathlabs.com (Tammy de Leon) Date: Thu Jul 21 15:33:49 2011 Subject: [Histonet] job openings Message-ID: If you're looking for a friendly environment, a new state-of-the-art LEED certified facility and an employer of choice, SPL is the place for you!! We are currently looking for qualified Histotechnicians/Histotechnologists and Lab Aides! Histo candidates should be an HTL or HT (ASCP) or equivalent. Primary responsibilities include on-site frozen sections including mobile laboratory units. SPL is CAP accredited, offers competitive pay, and a comprehensive benefits package. SPL pays 100% of employee premiums for Medical, Dental, LTD and Life! We also offer a Retirement Plan & Supplemental Insurance. Please forward resume to Tammy de Leon tammy@surgicalpathlabs.com Surgical Pathology Laboratory 800-304-1066 8455 66th Street N Pinellas Park, Florida 33781 www.surgicalpathlabs.com From AnthonyH <@t> chw.edu.au Thu Jul 21 19:06:41 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jul 21 19:06:56 2011 Subject: [Histonet] RE: RPM on Cytocentrifuge for CSF In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715718894F7F@xmdb02.nch.kids> 500rpm for 5 minutes using the Shandon Cytospin 3 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Fisher Sent: Friday, 22 July 2011 4:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RPM on Cytocentrifuge for CSF How long and at what RPM do you process your CSF cytocentrifuge specimens? Thank you. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From techmana12 <@t> yahoo.com Thu Jul 21 22:49:18 2011 From: techmana12 <@t> yahoo.com (Dorothy Glass) Date: Thu Jul 21 22:49:22 2011 Subject: [Histonet] 0.5% acid alcohol Message-ID: <1311306558.19022.YahooMailNeo@web114503.mail.gq1.yahoo.com> any one has the procedure to make 0.5% acid alcohol From AnthonyH <@t> chw.edu.au Thu Jul 21 22:57:46 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jul 21 22:57:54 2011 Subject: [Histonet] 0.5% acid alcohol In-Reply-To: <1311306558.19022.YahooMailNeo@web114503.mail.gq1.yahoo.com> References: <1311306558.19022.YahooMailNeo@web114503.mail.gq1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A715718895101@xmdb02.nch.kids> Dorothy, Usually made up in 70% ethanol using Hydrochloric acid ie: Ethanol 70ml Water 30ml HCl (Concentrated) 0.5ml But a recipe should be available for your 0.5% acid alcohol (since we are assuming HCL and 70% ethanol is used - could be any other acid or absolute rather than 70%) What is it to be used for? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Friday, 22 July 2011 1:49 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] 0.5% acid alcohol any one has the procedure to make 0.5% acid alcohol _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From histotech <@t> imagesbyhopper.com Fri Jul 22 06:06:19 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Jul 22 06:06:28 2011 Subject: [Histonet] Recycled alcohol Message-ID: Hi Histonetters! For those who use recycled alcohol, I have a few questions. Do you use it in your automatic H&E stainers? Are you having any troubles with proper, consistent H&E staining? We are using recycled alcohol, and wish to continue to do so IF we can. We are having issues with our staining - hematoxylin isn't as purple as it should be, eosin is way too pink/red. We use recycled alcohol when deparaffinizing and running down to water. For the record, we are also using recycled xylene. After staining, we use 2 recycled 100s and two fresh 100s. I have increased the hem time, decreased the eosin time, adjusted the acid alcohol and bluing... I'm tearing my hair out trying to troubleshoot this! Can anyone help me? Thanks! Michelle From Carol.Freeman <@t> utoledo.edu Fri Jul 22 06:37:54 2011 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Fri Jul 22 06:38:34 2011 Subject: [Histonet] ISH on ventana Message-ID: We our thinking about starting ISH on the ventana benchmark LT, we are currently using it for IHC and FISH, but I have been told that ISH is a little more difficult as far as validation and reproducability. Is there anyone who is familiar with this process on this machine and can offer any advice it would be truly appreciated. Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.freeman@utoledo.edu (419)383-5639 From JHowery2 <@t> yrmc.org Fri Jul 22 08:03:54 2011 From: JHowery2 <@t> yrmc.org (Howery, Jeffrey) Date: Fri Jul 22 08:03:57 2011 Subject: [Histonet] Job Opening Message-ID: We are currently looking to fill a position here at Yavapai Regional Medical Center. please check out our website at YRMC.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From paw555 <@t> yahoo.com Fri Jul 22 08:51:05 2011 From: paw555 <@t> yahoo.com (pam plumlee) Date: Fri Jul 22 08:51:10 2011 Subject: [Histonet] EDTA 14% Message-ID: <1311342665.83011.YahooMailNeo@web31903.mail.mud.yahoo.com> Good morning Histonetters:? I would like suggestions for a vendor that supplies 14% EDTA for a decal project our lab is starting.? Thanks for any help. ? Pam Plumlee H.T. (ASCP) Biotheranostics From sbaldwin <@t> mhhcc.org Fri Jul 22 09:33:36 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Fri Jul 22 09:33:24 2011 Subject: [Histonet] BENCCHMARK LT Message-ID: HISTONETTERS Has anyone pulled the drawer out on the benchmark while the machine is on with the pressure on? I know there is a fix but the customer service has not caalled us back!! Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From sbaldwin <@t> mhhcc.org Fri Jul 22 10:20:55 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Fri Jul 22 10:20:37 2011 Subject: [Histonet] BENCHMARK LT Message-ID: THANKS EVERYONE GOT IT GOING AGAIN APPRECIATE YOUR HELP Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From rjbuesa <@t> yahoo.com Fri Jul 22 10:26:32 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 22 10:26:35 2011 Subject: [Histonet] Recycled alcohol In-Reply-To: Message-ID: <1311348392.4719.YahooMailClassic@web65701.mail.ac4.yahoo.com> Issues with recycled ethanol and xylene appear frequently in this list and I always answer the same: IF the recycling unit is working adequately and is maintained well to assure a final (recycled) product of good quality there should be no differences because ethanol and xylene will be pure. The color of hematoxylin has nothing to do with ethanol although eosin may be influenced by water in the ethanol after staining. Now, if you want to simplify your dewaxing procedure, instead of xylene and ethanol dewax your sections using to baths of 2% aq. dishwasher soap at 90?C and you will be able to dewax much faster and cheaper, with optimum quality.Ren? J.? --- On Fri, 7/22/11, histotech@imagesbyhopper.com wrote: From: histotech@imagesbyhopper.com Subject: [Histonet] Recycled alcohol To: "Histonet@Lists. Utsouthwestern. Edu" Date: Friday, July 22, 2011, 7:06 AM Hi Histonetters! For those who use recycled alcohol, I have a few questions. Do you use it in your automatic H&E stainers? Are you having any troubles with proper, consistent H&E staining? We are using recycled alcohol, and wish to continue to do so IF we can.? We are having issues with our staining - hematoxylin isn't as purple as it should be, eosin is way too pink/red. We use recycled alcohol when deparaffinizing and running down to water. For the record, we are also using recycled xylene.? After staining, we use 2 recycled 100s and two fresh 100s. I have increased the hem time, decreased the eosin time, adjusted the acid alcohol and bluing... I'm tearing my hair out trying to troubleshoot this!? Can anyone help me? Thanks! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jul 22 10:28:06 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 22 10:28:10 2011 Subject: [Histonet] EDTA 14% In-Reply-To: <1311342665.83011.YahooMailNeo@web31903.mail.mud.yahoo.com> Message-ID: <1311348486.27765.YahooMailClassic@web65711.mail.ac4.yahoo.com> Why don't you prepare it in your lab? By doing so you will cut costs and assure a consistent decal solution. Ren? J. --- On Fri, 7/22/11, pam plumlee wrote: From: pam plumlee Subject: [Histonet] EDTA 14% To: "histonet@lists.utsouthwestern.edu" Date: Friday, July 22, 2011, 9:51 AM Good morning Histonetters:? I would like suggestions for a vendor that supplies 14% EDTA for a decal project our lab is starting.? Thanks for any help. ? Pam Plumlee H.T. (ASCP) Biotheranostics _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Fri Jul 22 10:40:53 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Jul 22 10:41:00 2011 Subject: [Histonet] Histotech Needed in PA Message-ID: *Allied Search Partners is currently looking for a qualified Histotech for a laboratory in Lancaster, PA. We have a temporary or permanent position available. We are looking for local candidates only for the temp position, and the position would be effective August 1st. We are also looking for a long term permanent histotech too. * * * *Position: *Histotechnologist or Histotechnician * * *Location:* Lancaster, PA * * *Schedule: *We have either a position for a Temporary Histotech (local candidates only) OR a Permanent Histotech (Full Time/Direct Hire, M-F Day Shift). When applying please indicate which one you are interested in. * * *Requirements & Summary:* Permanent Position/Direct Hire 3-5+ years experience HTL/HT ASCP QIHC ASCP certification a plus IHC experience preferred * * *Benefits:* PTO- 3 weeks plus 6 holidays 100% paid health insurance for employee and dependents 15$/month Dental plan for single person (Optional) 401(k) plan - eligible after one year of employment and vested after 1 year * * *To Apply:* Please send resume alyssa@alliedsearchpartners.com to schedule a phone screen with one of our recruiters. -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From gayle.callis <@t> bresnan.net Fri Jul 22 11:48:40 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jul 22 11:48:48 2011 Subject: [Histonet] Re: 14% EDTA Message-ID: <000001cc488f$379b7580$a6d26080$@bresnan.net> There is no vendor I know of who sells 14% EDTA. It is important to remember that the EDTA you have to use is Tetrasodium EDTA, MW 452.2. This particular EDTA is highly soluble in water compared to EDTA without any sodiums, or disodium EDTA. Also, once dissolved it has a very high pH of approximately 9 or more and you must adjust the pH DOWN to pH 7.2 - 7.4, although we use pH 7.6 without problems. If you do not adjust the pH down, you will damage alkaline sensitive protein linkages in your tissues/bone. Do not use HCl to adjust the pH, but glacial acetic acid. The publication supporting this EDTA decalcification was written by Dr. Webb Jee many years ago. We prefer to use tetrasodium EDTA because we can achieve a high concentration with the highly soluble EDTA. You can buy tetrasodium EDTA if from any vendor, ThermoScientific, VWR, etc. Simple recipe is 14 g Tetrasodium EDTA mw 452.2 (this may vary) 80 ml Dulbeccos PBS or water (the latter is fine IF your bones are totally fixed) Adjust pH DOWN to 7.4 with glacial acetic acid (it takes a fairly large volume) Bring final volume to 100 ml with either buffer or water, depending on what you dissolved the salts in. We adjust pH using constant stirring and pH electrode in the stock solution to watch the pH change. Keep track of final volume of glacial acetic acid, so the next time you make this up, you can add a large volume without having to keep adding glacial acetic acid with a pipette (in sense, a titration to correct pH). As with all EDTA decalcification, it takes longer and you must rinse the bones well for a couple of hours or the EDTA will ppt out in alcohols, creating some annoying sectioning problems. Suspend your bones in this solution and use endpoint testing to know when calcium is removed. We use a simple weight loss weight gain endpoint test that works very well for EDTA unless you have an xray machine or microCT scanner to detect Ca removal. EDTA is not affected by temperature, and can be performed in the cold although room temperature works perfectly. Gayle M. Callis HTL/HT/MT(ASCP) From scampbell <@t> celligent.net Fri Jul 22 12:01:18 2011 From: scampbell <@t> celligent.net (Campbell, Sharon) Date: Fri Jul 22 11:57:25 2011 Subject: [Histonet] NBF waste disposal Message-ID: <4B869E3F108B034DAE3A55AB76AB15A011BDFAC5C7@VA3DIAXVS781.RED001.local> Happy Friday everyone, I am currently researching ways to dispose of NBF waste. I would like to know if you are neutralizing the waste and then putting it down the drain or if you are shipping it off to be handled by another company. I am looking for the best and most cost effective way to handle this waste. We are not in a position to reclaim the formalin as space is not available for the still. Thank you for your help on this. Sharon Campbell Sharon Campbell HT, HTL (ASCP) Histology Supervisor Celligent Diagnostics, LLC 106 Venture Blvd. Spartanburg, SC 29306 (864) 583-3850 From thiggins <@t> cddmedical.com Fri Jul 22 12:38:32 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Fri Jul 22 12:38:59 2011 Subject: [Histonet] Re: Recycled alcohol References: <20110722170341.46053147E3EB@barracuda.crvinc.net> Message-ID: <000501cc4896$2dadf050$e001a8c0@cdd.loc> Hey Michelle, The easiest way to find out if it's the recycled regeants is to use fresh reagents and see how the stain looks. Obvisously if it still shows sign of a lack of color or detail than I would think it could be the stains themselves. What manufacture and type of Hematoxylin and Eosin are you using? Thanks, Tim Message: 9 Date: Fri, 22 Jul 2011 07:06:19 -0400 From: "histotech@imagesbyhopper.com" Subject: [Histonet] Recycled alcohol To: "Histonet@Lists. Utsouthwestern. Edu" Message-ID: Content-Type: text/plain; charset=us-ascii Hi Histonetters! For those who use recycled alcohol, I have a few questions. Do you use it in your automatic H&E stainers? Are you having any troubles with proper, consistent H&E staining? We are using recycled alcohol, and wish to continue to do so IF we can. We are having issues with our staining - hematoxylin isn't as purple as it should be, eosin is way too pink/red. We use recycled alcohol when deparaffinizing and running down to water. For the record, we are also using recycled xylene. After staining, we use 2 recycled 100s and two fresh 100s. I have increased the hem time, decreased the eosin time, adjusted the acid alcohol and bluing... I'm tearing my hair out trying to troubleshoot this! Can anyone help me? Thanks! Michelle From histotech <@t> imagesbyhopper.com Fri Jul 22 13:47:22 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Jul 22 13:47:40 2011 Subject: [Histonet] NBF waste disposal In-Reply-To: <4B869E3F108B034DAE3A55AB76AB15A011BDFAC5C7@VA3DIAXVS781.RED001.local> References: <4B869E3F108B034DAE3A55AB76AB15A011BDFAC5C7@VA3DIAXVS781.RED001.local> Message-ID: <0530092C-3F15-4288-95B4-D414E622BF7F@imagesbyhopper.com> Sharon, We use a product called Aldex to neutralize our formalin. It binds with the aldehyde groups and neutralizes them. According to the mfr, you can dispose of it in regular trash, but I am more cautious and throw it away in red bag trash. Michelle Sent from my iPhone On Jul 22, 2011, at 1:01 PM, "Campbell, Sharon" wrote: > Happy Friday everyone, > I am currently researching ways to dispose of NBF waste. I would like to know if you are neutralizing the waste and then putting it down the drain or if you are shipping it off to be handled by another company. I am looking for the best and most cost effective way to handle this waste. We are not in a position to reclaim the formalin as space is not available for the still. > Thank you for your help on this. > > Sharon Campbell > > Sharon Campbell HT, HTL (ASCP) > Histology Supervisor > > Celligent Diagnostics, LLC > 106 Venture Blvd. > Spartanburg, SC 29306 > (864) 583-3850 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Fri Jul 22 14:38:07 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Fri Jul 22 14:37:50 2011 Subject: [Histonet] NBF disposal Message-ID: I am in Indiana we got inspected IDEM and they said even if you neutralize the formalin it is not suppose to be put down the drain, none the less we now have this hauled off. We also cannot put ETOH down the drain as well. Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From rjbuesa <@t> yahoo.com Fri Jul 22 14:50:44 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 22 14:50:47 2011 Subject: [Histonet] NBF waste disposal In-Reply-To: <4B869E3F108B034DAE3A55AB76AB15A011BDFAC5C7@VA3DIAXVS781.RED001.local> Message-ID: <1311364244.666.YahooMailClassic@web65707.mail.ac4.yahoo.com> I have tried ALL available neutralizing agents in the market and ALL of them test positive for formalin after the neutralization is completed. I would not dump any "neutralized" formalin down the drain. After all those tests I ended returning to my reliable method of having someone to haul it out even when, according to the Florida law, I am liable for it no matter who takes care of dispose of it. Ren? J. --- On Fri, 7/22/11, Campbell, Sharon wrote: From: Campbell, Sharon Subject: [Histonet] NBF waste disposal To: "histonet@lists.utsouthwestern.edu" Date: Friday, July 22, 2011, 1:01 PM Happy Friday everyone, I am currently researching ways to dispose of NBF waste. I would like to know if you are neutralizing the waste and then putting it down the drain or if you are shipping it off to be handled by another company. I am looking for the best and most cost effective way to handle this waste. We are not in a position to reclaim the formalin as space is not available for the still. Thank you for your help on this. Sharon Campbell Sharon Campbell HT, HTL (ASCP) Histology Supervisor Celligent Diagnostics, LLC 106 Venture Blvd. Spartanburg, SC? 29306 (864) 583-3850 -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Fri Jul 22 14:55:16 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Jul 22 14:55:19 2011 Subject: [Histonet] RE: NBF waste disposal In-Reply-To: <4B869E3F108B034DAE3A55AB76AB15A011BDFAC5C7@VA3DIAXVS781.RED001.local> References: <4B869E3F108B034DAE3A55AB76AB15A011BDFAC5C7@VA3DIAXVS781.RED001.local> Message-ID: Sharon, IMO - in today's world no laboratory waste should "go down the drain". It is just not an environmentally responsible practice - and it all ends up eventually be discharged into in a stream somewhere. We collect all liquid waste (formalin, ETOH, xylenes, acids, bases, stains, buffers...you name it) and contract with CleanHarbors http://cleanharbors.com/ for disposal. Collection and proper disposal IS the best practice. Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Sharon Sent: Friday, July 22, 2011 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NBF waste disposal Happy Friday everyone, I am currently researching ways to dispose of NBF waste. I would like to know if you are neutralizing the waste and then putting it down the drain or if you are shipping it off to be handled by another company. I am looking for the best and most cost effective way to handle this waste. We are not in a position to reclaim the formalin as space is not available for the still. Thank you for your help on this. Sharon Campbell Sharon Campbell HT, HTL (ASCP) Histology Supervisor Celligent Diagnostics, LLC 106 Venture Blvd. Spartanburg, SC 29306 (864) 583-3850 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From jclark <@t> pcnm.com Fri Jul 22 15:22:42 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Fri Jul 22 15:30:15 2011 Subject: [Histonet] Paraffin Wax Waste Disposal Message-ID: <0494A7D4E8CC254EA2FB81464982E3786E8D9A@S10MAILD001N1.SH10.lan> Hi All, we had our CAP inspection yesterday and were cited for disposing of our waste wax from the processors in regular waste. In all my 20+ years of working in histology I have never disposed of the dirty wax in biohazard waste. Especially now with the newer processors that have very little carry over. I know this is probably state regulated by is anyone aware of a regulation or documentation that states what the amount of hazardous chemical in a substance must be before it is considered hazardous? And if so, does anyone know of a way to measure the amount of xylene in waste paraffin? Thanks in advance. Joanne Clark, HT Histology Supervisor PCNM From MLunetta <@t> luhcares.org Fri Jul 22 15:38:53 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Fri Jul 22 15:39:17 2011 Subject: [Histonet] Re: RPM on Cytocentrifuge for CSF Message-ID: <4E298B7D020000A800061FAD@ns.luhcares.org> We have ours set at 750 RPM for 2 Min. Matt Lunetta BS,HT(ASCP) Longmont United Hospital ------------------------------ Message: 2 Date: Thu, 21 Jul 2011 18:30:44 +0000 From: Marcia Fisher Subject: [Histonet] RPM on Cytocentrifuge for CSF To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" How long and at what RPM do you process your CSF cytocentrifuge specimens? Thank you. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.org From marktarango <@t> gmail.com Fri Jul 22 16:19:45 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Jul 22 16:19:50 2011 Subject: [Histonet] Paraffin Wax Waste Disposal In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3786E8D9A@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3786E8D9A@S10MAILD001N1.SH10.lan> Message-ID: That's funny that you got cited for that. I was surprised to learn what our safety officer setup at my lab for paraffin disposal. A commercial company takes our paraffin and makes a product with it that is mixed with cedar sawdust and paraffin wax for starting fires in the BBQ, fireplace, or campfires, etc. I wonder if consumers would want to use this product knowing where the paraffin came from. The company tested the paraffin and said it's pure enough to meet their standards. Just thought it was an interesting so I decided to share. Mark On Fri, Jul 22, 2011 at 1:22 PM, Joanne Clark wrote: > Hi All, we had our CAP inspection yesterday and were cited for disposing of > our waste wax from the processors in regular waste. In all my 20+ years of > working in histology I have never disposed of the dirty wax in biohazard > waste. Especially now with the newer processors that have very little carry > over. I know this is probably state regulated by is anyone aware of a > regulation or documentation that states what the amount of hazardous > chemical in a substance must be before it is considered hazardous? And if > so, does anyone know of a way to measure the amount of xylene in waste > paraffin? > > Thanks in advance. > Joanne Clark, HT > Histology Supervisor > PCNM > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MSHERWOOD <@t> PARTNERS.ORG Fri Jul 22 16:31:18 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Jul 22 16:31:23 2011 Subject: [Histonet] Paraffin Wax Waste Disposal In-Reply-To: References: <0494A7D4E8CC254EA2FB81464982E3786E8D9A@S10MAILD001N1.SH10.lan> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB58EE@PHSXMB30.partners.org> We have to have our processor paraffin picked up as hazardous waste, since it has traces of CitriSolv in it. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, July 22, 2011 5:20 PM To: Joanne Clark Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Wax Waste Disposal That's funny that you got cited for that. I was surprised to learn what our safety officer setup at my lab for paraffin disposal. A commercial company takes our paraffin and makes a product with it that is mixed with cedar sawdust and paraffin wax for starting fires in the BBQ, fireplace, or campfires, etc. I wonder if consumers would want to use this product knowing where the paraffin came from. The company tested the paraffin and said it's pure enough to meet their standards. Just thought it was an interesting so I decided to share. Mark On Fri, Jul 22, 2011 at 1:22 PM, Joanne Clark wrote: > Hi All, we had our CAP inspection yesterday and were cited for disposing of > our waste wax from the processors in regular waste. In all my 20+ years of > working in histology I have never disposed of the dirty wax in biohazard > waste. Especially now with the newer processors that have very little carry > over. I know this is probably state regulated by is anyone aware of a > regulation or documentation that states what the amount of hazardous > chemical in a substance must be before it is considered hazardous? And if > so, does anyone know of a way to measure the amount of xylene in waste > paraffin? > > Thanks in advance. > Joanne Clark, HT > Histology Supervisor > PCNM > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From kiran_g <@t> sbcglobal.net Fri Jul 22 18:19:31 2011 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Fri Jul 22 18:19:35 2011 Subject: [Histonet] Tissue Processing: Blue Dye used to see small tissue?? Message-ID: <1311376771.91935.YahooMailClassic@web180103.mail.gq1.yahoo.com> Hi, ? Please let me know the name and ordering information of the blue dye used during processing. Currently we use eosin in all our alcohols but still tissues are very pale and hard to see at embedding. ? Please share If anyone has any other sugesstions that will help to see small tissues better at embedding. ? ? Thank you and Happy Friday! ? -K From anastasiapinkston <@t> hotmail.com Fri Jul 22 18:51:08 2011 From: anastasiapinkston <@t> hotmail.com (Anastasia Pinkston) Date: Fri Jul 22 18:51:12 2011 Subject: [Histonet] RE: Histonet Digest, Vol 92, Issue 29 In-Reply-To: References: Message-ID: Kevin, This reply has nothing to do with your post. But my family and I will be moving to Tallahassee Florida soon and I would really like to speak with regrading Histology in that area. Would you please email me at anastasiapinkston@hotmail.com at your convenience? Ana Pinkston > Greetings, > > ? > > My name is Kevin and I've been working in a pathology? / clinical lab setting for the past 8 yrs. > > I've recently heard about veterinary histology and the possibilities seem endless.? My initial searches? have not been very productive and I've even heard that certain states do not allow such studies....? Is this true ?? if so, which ones...? > > ? > > I currently live in Tallahassee, Florida and? will be re-locating east ( Jacksonville - Daytona Beach, Fl? ) in the near future. > > ? > > Can anyone help enlighten me ?? Any and all help would be greatly appreciated. > > ? > > God Bless, > > ? > > Kevin R. Evelyn > > KWB Pathology Assoc > > Tallahassee, Florida From dbpiontek <@t> hotmail.com Sat Jul 23 01:22:46 2011 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Sat Jul 23 01:22:50 2011 Subject: [Histonet] Re: Message-ID: How to lose weight fast.... http://saudi1sale.com/valuable.php?olSID=95a3 From techmana12 <@t> yahoo.com Sat Jul 23 08:31:55 2011 From: techmana12 <@t> yahoo.com (Dorothy Glass) Date: Sat Jul 23 08:32:02 2011 Subject: [Histonet] ki67/mart 1 double stain Message-ID: <1311427915.28773.YahooMailNeo@web114520.mail.gq1.yahoo.com> any sugestion on validating ki67/mart 1 on the Ventana XT or Ultra.? I need a protocol to start. From rjbuesa <@t> yahoo.com Sat Jul 23 09:46:47 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 23 09:46:51 2011 Subject: [Histonet] Paraffin Wax Waste Disposal In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3786E8D9A@S10MAILD001N1.SH10.lan> Message-ID: <1311432407.40714.YahooMailClassic@web65713.mail.ac4.yahoo.com> I always incinerated my used paraffin Ren? J --- On Fri, 7/22/11, Joanne Clark wrote: From: Joanne Clark Subject: [Histonet] Paraffin Wax Waste Disposal To: "histonet@lists.utsouthwestern.edu" Date: Friday, July 22, 2011, 4:22 PM Hi All, we had our CAP inspection yesterday and were cited for disposing of our waste wax from the processors in regular waste.? In all my 20+ years of working in histology I have never disposed of the dirty wax in biohazard waste.? Especially now with the newer processors that have very little carry over.? I know this is probably state regulated by is anyone aware of a regulation or documentation that states what the amount of hazardous chemical in a substance must be before it is considered hazardous?? And if so, does anyone know of a way to measure the amount of xylene in waste paraffin? Thanks in advance. Joanne Clark, HT Histology Supervisor PCNM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Sat Jul 23 10:56:05 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Sat Jul 23 10:56:10 2011 Subject: [Histonet] Recycled alcohol Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3679170B@PEITHA.wad.pa-ucl.com> Message: 9 Date: Fri, 22 Jul 2011 07:06:19 -0400 From: "histotech@imagesbyhopper.com" Subject: [Histonet] Recycled alcohol To: "Histonet@Lists. Utsouthwestern. Edu" Message-ID: Content-Type: text/plain; charset=us-ascii Hi Histonetters! For those who use recycled alcohol, I have a few questions. Do you use it in your automatic H&E stainers? Are you having any troubles with proper, consistent H&E staining? We are using recycled alcohol, and wish to continue to do so IF we can. We are having issues with our staining - hematoxylin isn't as purple as it should be, eosin is way too pink/red. We use recycled alcohol when deparaffinizing and running down to water. For the record, we are also using recycled xylene. After staining, we use 2 recycled 100s and two fresh 100s. I have increased the hem time, decreased the eosin time, adjusted the acid alcohol and bluing... I'm tearing my hair out trying to troubleshoot this! Can anyone help me? Thanks! Michelle We test and use our recycled alcohol as 95% alcohol - we do not sub it for the 100%. We also use recycled xylene with no issues. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From tallyguy555 <@t> comcast.net Sat Jul 23 13:14:56 2011 From: tallyguy555 <@t> comcast.net (tallyguy555@comcast.net) Date: Sat Jul 23 13:14:59 2011 Subject: [Histonet] Histo Tech seeks job in Florida Message-ID: <1211837510.173771.1311444896042.JavaMail.root@sz0168a.westchester.pa.mail.comcast.net> Received initial training in Denver, Co. and?have been employed in Tallahassee for the past 6 yrs. Ideal position would ibe a firs t sh ift position with general bench work.?Strong grosser if needed. My areas of interests are the east coast ( Jacksonville down to New Smyrna Beach ) From techmana12 <@t> yahoo.com Sun Jul 24 00:28:04 2011 From: techmana12 <@t> yahoo.com (Dorothy Glass) Date: Sun Jul 24 00:28:09 2011 Subject: Fw: [Histonet] ki67/mart 1 double stain In-Reply-To: <1311427915.28773.YahooMailNeo@web114520.mail.gq1.yahoo.com> References: <1311427915.28773.YahooMailNeo@web114520.mail.gq1.yahoo.com> Message-ID: <1311485284.58956.YahooMailNeo@web114505.mail.gq1.yahoo.com> anyone using ki67/mart 1 double stain have a protocol to share on histonet? ----- Forwarded Message ----- From: Dorothy Glass To: "Histonet@lists.utsouthwestern.edu" Sent: Saturday, July 23, 2011 9:31 AM Subject: [Histonet] ki67/mart 1 double stain any sugestion on validating ki67/mart 1 on the Ventana XT or Ultra.? I need a protocol to start. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Milton.Gomez <@t> nyumc.org Sun Jul 24 09:59:03 2011 From: Milton.Gomez <@t> nyumc.org (Gomez, Milton) Date: Sun Jul 24 09:59:13 2011 Subject: [Histonet] PPE Message-ID: <4A53F9A1D7C2674FA4A6E650D703DDA5FEA15592@MSGWSDCPMB07.nyumc.org> Hello Histonetters, Is it up to the laboratory director to decide what kind of PPE histologists wear in the lab and during which rotations? Techs are currently wearing a lab coat while cutting and embedding and it gets extremely hot. Can they just wear an apron while cutting and an apron and sleeves while embedding?. They wear it because the Hospital Safety Officer thinks it is what needs to be done to comply with State and Federal Regulations. I feel the laboratory director can overrule the Safety Officer's decision for the well being of the lab techs. Where can I find more information on this issue to show my director and safety officer? Thanks so much in advance, MG ------------------------------------------------------------
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From tpodawiltz <@t> lrgh.org  Sun Jul 24 13:12:47 2011
From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas)
Date: Sun Jul 24 13:14:04 2011
Subject: [Histonet] Paraffin Wax Waste Disposal
In-Reply-To: <1311432407.40714.YahooMailClassic@web65713.mail.ac4.yahoo.com>
References: <0494A7D4E8CC254EA2FB81464982E3786E8D9A@S10MAILD001N1.SH10.lan>,
	<1311432407.40714.YahooMailClassic@web65713.mail.ac4.yahoo.com>
Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE4E@LRGHEXVS1.practice.lrgh.org>

We dispose of our waste paraffin as a chemical waste. It gets hauled away, since we have no way to incinerate it.



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Saturday, July 23, 2011 10:46 AM
To: histonet@lists.utsouthwestern.edu; Joanne Clark
Subject: Re: [Histonet] Paraffin Wax Waste Disposal

I always incinerated my used paraffin
Ren? J

--- On Fri, 7/22/11, Joanne Clark  wrote:


From: Joanne Clark 
Subject: [Histonet] Paraffin Wax Waste Disposal
To: "histonet@lists.utsouthwestern.edu" 
Date: Friday, July 22, 2011, 4:22 PM


Hi All, we had our CAP inspection yesterday and were cited for disposing of our waste wax from the processors in regular waste.  In all my 20+ years of working in histology I have never disposed of the dirty wax in biohazard waste.  Especially now with the newer processors that have very little carry over.  I know this is probably state regulated by is anyone aware of a regulation or documentation that states what the amount of hazardous chemical in a substance must be before it is considered hazardous?  And if so, does anyone know of a way to measure the amount of xylene in waste paraffin?

Thanks in advance.
Joanne Clark, HT
Histology Supervisor
PCNM
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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From Milton.Gomez <@t> nyumc.org  Mon Jul 25 04:43:31 2011
From: Milton.Gomez <@t> nyumc.org (Gomez, Milton)
Date: Mon Jul 25 04:44:49 2011
Subject: [Histonet] Microtome draft shield
In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707030FFC@SMCMAIL01.somerset-healthcare.com>
References: <4E244F60.C0AD.00BF.1@inspection.gc.ca>,
	<3AD061FE740D464FAC7BF6B5CFB75707030FFC@SMCMAIL01.somerset-healthcare.com>
Message-ID: <4A53F9A1D7C2674FA4A6E650D703DDA5040957D3FD@MSGWSDCPMB07.nyumc.org>

negative pressure will make the ribbons have a flying party.  is it feassible to built a room within the lab with no pressure for sectioning?

mg

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni [trathborne@somerset-healthcare.com]
Sent: Tuesday, July 19, 2011 9:11 AM
To: 'Keri Colwell'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome draft shield

Haven't seen anything that specific, but you might want to look at the various biohazard splash guards. They are a clear Plexiglas, and they have a base to support them. You would be able to move them around (or have them mounted to the counter if desired), and they come in an assortment of angles and sizes.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keri Colwell
Sent: Monday, July 18, 2011 5:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome draft shield

Hi Everyone,

I work in a lab which is under negative pressure (air is continuously flowing in), and due to the layout of the rooms are microtomes are located next to two different doorways.  We are looking for some sort of draft shield to place around each microtome and water bath that will reduce the effects of the airflow and personnel movements on our ribbons.

Anyone have any suggestions as to who might sell such a thing?

Thanks in advance!




Keri Colwell
Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640  | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone:  403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada

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From histotech <@t> imagesbyhopper.com  Mon Jul 25 06:29:26 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Mon Jul 25 06:29:36 2011
Subject: [Histonet] H&E protocol question
Message-ID: <8DDCDA33-B24D-498E-AA0B-6DCB4B436439@imagesbyhopper.com>

Hi Histonetters!

I'm looking for thoughts on preferences/pros/cons between using a progressive and a regressive H&E on routine daily work.

Which hematoxylins do you prefer (commercially prepared), which eosin?

Anyone have a tried and true protocol for each method?

Thanks!

Michelle

Sent from my iPhone
From member <@t> linkedin.com  Mon Jul 25 06:42:44 2011
From: member <@t> linkedin.com (Thomas Huynh via LinkedIn)
Date: Mon Jul 25 06:42:47 2011
Subject: [Histonet] Invitation to connect on LinkedIn
Message-ID: <916187402.3771933.1311594164624.JavaMail.app@ela4-bed39.prod>

LinkedIn
------------




    Thomas Huynh requested to add you as a connection on LinkedIn:
  
------------------------------------------

David,

I'd like to add you to my professional network on LinkedIn.

- Thomas

Accept invitation from Thomas Huynh
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From dmattingly90 <@t> gmail.com  Mon Jul 25 06:46:57 2011
From: dmattingly90 <@t> gmail.com (Denise Mattingly)
Date: Mon Jul 25 06:47:00 2011
Subject: [Histonet] Wax disposal
Message-ID: 

Are the disposal issues over the
Used paraffin because of chemicals like xylene in the wax
Or is it the human tissue in the
Wax
From Lisa.White3 <@t> va.gov  Mon Jul 25 07:24:02 2011
From: Lisa.White3 <@t> va.gov (White, Lisa M.)
Date: Mon Jul 25 07:24:06 2011
Subject: [Histonet] Tissue Processing: Blue Dye used to see small tissue
Message-ID: <2B2ECF33934F5D4996D8BE03EFDF3976085F4ED2@VHAV09MSGA3.v09.med.va.gov>

We use Mucicarmine placed in a small dropper bottle, it does not wash
out in the processor and makes the tissues easy to see once embedded.

 

 

Lisa White, HT(ASCP)

Supervisory HT

James H. Quillen VAMC

PO Box 4000

Corner of Veterans Way and Lamont

PLMS 113

Mountain Home, TN 37684

423-979-3567

423-979-3401 fax

 

From Dorothy.L.Webb <@t> HealthPartners.Com  Mon Jul 25 07:44:59 2011
From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L)
Date: Mon Jul 25 07:45:03 2011
Subject: [Histonet] Recycled alcohol
Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43012203348E77@HPEMX3.HealthPartners.int>

You do not get 100% alcohol back from recycling, so it cannot be used as such in either the processor or stainer.  Basically, you have to use it as 95%.  That may be your problem!!



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From arsenn <@t> hsh.org  Mon Jul 25 07:53:46 2011
From: arsenn <@t> hsh.org (Senn, Amy R)
Date: Mon Jul 25 07:54:40 2011
Subject: [Histonet] NBF & Recycled Alc
Message-ID: 

Hi all,

We dump everything (and I mean EVERYTHING) down the drain.  Formalin,
waste from Benchmark, etc.

 

We use 95% recycled alcohol for everything as well (stainer, making up
reagents, etc), but that's the only reagent (other than clear-rite) that
we use recycled. Our 100% alcohol is right from the bottle.

We check our 95 also, and if it's not a 'true' 95, it gets dumped.

 

Thanks

 

Amy R.

Holy Spirit Health System

503 N. 21st Street

Camp Hill, PA 17011

Phone: 717-763-2124

Fax: 717-763-2947

www.hsh.org



 

 



Attention:  This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you.
From sbaldwin <@t> mhhcc.org  Mon Jul 25 08:03:50 2011
From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org)
Date: Mon Jul 25 08:03:35 2011
Subject: [Histonet] PARAFFIN WASTE
Message-ID: 

Our paraffin is also hauled off with hazardous waste
Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-482-0210, 0216,  Fax 812-482-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
From sbaldwin <@t> mhhcc.org  Mon Jul 25 08:25:55 2011
From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org)
Date: Mon Jul 25 08:25:35 2011
Subject: [Histonet] ki67/mart 1 double stain
Message-ID: 

I  have  a protocol I will share from the benchmark lt  for dual stains if you want to e-mail me directly!
 
Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-482-0210, 0216,  Fax 812-482-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
From bboyce <@t> NEMOURS.ORG  Mon Jul 25 09:30:48 2011
From: bboyce <@t> NEMOURS.ORG (Boyce, Bobbie)
Date: Mon Jul 25 09:30:52 2011
Subject: [Histonet] Frozen eye problems
In-Reply-To: 
References: 
Message-ID: 

Hello Histoneters,

I'm having problems getting a good section on paraformaldyehyde fixed
adult mouse eyes frozen in TBS tissue freezing medium. One of the
problems was the orentation of them. They were too close to the edge. I
also, wanted to try a harder freezing medium, so I corrected oriented
and used an OCT equivalent tissue freezing medium.  They looked better,
but still not as nice as I think they should be.

My eye contact tells me that they should be cut at -20 for adult and -14
for neonatal. They have me set the knife angle at 0 to -2.5. 

I also tried cutting these as I would cut any other frozen issue, but
I'm just pulling my hair out.

Does anyone have any other suggestions that I might try? Your help would
be greatly appreciated.

Bobbie Boyce
Histology Specialist III
duPont Hospital for Children
Wilmington, DE
302-651-6771 (Lab)
302-651-5010 (Fax)





From AGleiberman <@t> cbiolabs.com  Mon Jul 25 09:47:14 2011
From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman)
Date: Mon Jul 25 09:47:23 2011
Subject: [Histonet] RE: Frozen eye problems
In-Reply-To: 
References: 
	
Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C37E11A@cbiolabs05.CBiolabs.local>

Try Neg-50 (Fisher, cat#22-110-617) instead of OCT, we don't have any problem with fresh-frozen mouse eyes.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman@cbiolabs.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyce, Bobbie
Sent: Monday, July 25, 2011 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen eye problems

Hello Histoneters,

I'm having problems getting a good section on paraformaldyehyde fixed
adult mouse eyes frozen in TBS tissue freezing medium. One of the
problems was the orentation of them. They were too close to the edge. I
also, wanted to try a harder freezing medium, so I corrected oriented
and used an OCT equivalent tissue freezing medium.  They looked better,
but still not as nice as I think they should be.

My eye contact tells me that they should be cut at -20 for adult and -14
for neonatal. They have me set the knife angle at 0 to -2.5. 

I also tried cutting these as I would cut any other frozen issue, but
I'm just pulling my hair out.

Does anyone have any other suggestions that I might try? Your help would
be greatly appreciated.

Bobbie Boyce
Histology Specialist III
duPont Hospital for Children
Wilmington, DE
302-651-6771 (Lab)
302-651-5010 (Fax)





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 


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From rjbuesa <@t> yahoo.com  Mon Jul 25 10:38:02 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Mon Jul 25 10:38:06 2011
Subject: [Histonet] H&E protocol question
In-Reply-To: <8DDCDA33-B24D-498E-AA0B-6DCB4B436439@imagesbyhopper.com>
Message-ID: <1311608282.26873.YahooMailClassic@web65701.mail.ac4.yahoo.com>

Progressive hematoxylins usually take more time to stain? and for routine work the schedule has to be very "fine tuned" because otherwise obtaining the ideal staining is quite difficult with a frequent tendency to the "under staining".
Regressive hematoxylins on the other hand, always over stain and then you can obtain the ideal staining by removing the hematoxylin with acid or aqueous solutions. They are faster and the results are more easy to control.
For many years I always prepared my hematoxylins, but now there are commercial solutions of great quality?and consistent?results so it is better (although no more cost effective) to buy a reputable brand, develop a good protocol and stick to it. At the end I relied on Richard Allen hematoxylins.
Eosin, on the other hand, are so easy to prepare that I always prepared mine.
Ren? J.

--- On Mon, 7/25/11, histotech@imagesbyhopper.com  wrote:


From: histotech@imagesbyhopper.com 
Subject: [Histonet] H&E protocol question
To: "Histonet@Lists. Utsouthwestern. Edu" 
Date: Monday, July 25, 2011, 7:29 AM


Hi Histonetters!

I'm looking for thoughts on preferences/pros/cons between using a progressive and a regressive H&E on routine daily work.

Which hematoxylins do you prefer (commercially prepared), which eosin?

Anyone have a tried and true protocol for each method?

Thanks!

Michelle

Sent from my iPhone
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From sbaldwin <@t> mhhcc.org  Mon Jul 25 11:01:39 2011
From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org)
Date: Mon Jul 25 11:01:21 2011
Subject: [Histonet] DS MART 1/ KI 67
Message-ID: 

  
FOR
BENNCHMARK LT
Procedure  XT IHC DS UDAB-URED
Depar
Cell condition short 8 min
37 degrees/default
Mart 1 32 min
Amplify
ultrawash
aby denaturation
90 deg C  4 min
37 degrees/default 
Ki67  4min
DS ultra wash 
counterstain Heme (we do 20 min on this our Path likes it dark)
usually Heme is 4  min



Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-482-0210, 0216,  Fax 812-482-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
From LSebree <@t> uwhealth.org  Mon Jul 25 11:39:57 2011
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Mon Jul 25 11:40:00 2011
Subject: [Histonet] Working Ventana Probe Protocols (EBER)
Message-ID: 

Hi Marc and Histonetters (especially those using XTs),
 
 
I've been trying to work up VMS's new EBER probe with less than stellar
results.
 
I have some questions about the protocols you sent via Histonet back in
early June.  My questions/protocols are in red.
 
You state the following:
 
Depar 16 min  We can only select "depar" without an incubation
time....does your instrument allow you to select a time?  We are doing
this on an XT, is this an instrument difference?  What instrument are
you running your ISH on....Ultra by chance?
 
Enzyme Protease 2 for 4 min. We are using ISH Protease 2 / 12 mins. as
that was our protocol with the "old" stuff.
 
ISH (EBER) probe 4 min. Same
 
Denature @ 85 degrees for 12 min. Same
 
Hybe 1 hour What temperature are you hybridizing at? 
 
3 stringency washes for 8 minutes each At what temperature?
 
Blue detection for 20 minutes Same
 
Counterstain for 4 min We use Nuclear Fast Red for 8 mins.
 
Marc, are you using HybReady?
 
Anyone else having had some success with the new EBER probe, reagents
and software, feel free to comment.  Of the 3 specimens I've run, known
positive soft tissue, bm bx and cell block, the soft tissue and bm bx
had some positivity with the EBER DNP and U6 DNP but the cell block was
negative with both.  There was also lots of blue smearing and haze over
the slides.  I'm afraid this optimization/validation could end up being
very expensive even with our 3 control tissues picked up together on
single slides.
 
Thanks for everyone's help,
 
Linda
 

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
DB1-223 VAH 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 

 
From marktarango <@t> gmail.com  Mon Jul 25 11:48:57 2011
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Mon Jul 25 11:49:01 2011
Subject: [Histonet] Working Ventana Probe Protocols (EBER)
In-Reply-To: 
References: 
Message-ID: 

Hi Linda,

I had simliar trouble before I bought and added the extra HybReady to the
protocol (there is one that comes with the detection kit, but you need two
for good staining).  It will clean up your stain and A LOT and remove those
blue splotches that show up when you don't use it.

Mark

On Mon, Jul 25, 2011 at 9:39 AM, Sebree Linda A wrote:

> Hi Marc and Histonetters (especially those using XTs),
>
>
> I've been trying to work up VMS's new EBER probe with less than stellar
> results.
>
> I have some questions about the protocols you sent via Histonet back in
> early June.  My questions/protocols are in red.
>
> You state the following:
>
> Depar 16 min  We can only select "depar" without an incubation
> time....does your instrument allow you to select a time?  We are doing
> this on an XT, is this an instrument difference?  What instrument are
> you running your ISH on....Ultra by chance?
>
> Enzyme Protease 2 for 4 min. We are using ISH Protease 2 / 12 mins. as
> that was our protocol with the "old" stuff.
>
> ISH (EBER) probe 4 min. Same
>
> Denature @ 85 degrees for 12 min. Same
>
> Hybe 1 hour What temperature are you hybridizing at?
>
> 3 stringency washes for 8 minutes each At what temperature?
>
> Blue detection for 20 minutes Same
>
> Counterstain for 4 min We use Nuclear Fast Red for 8 mins.
>
> Marc, are you using HybReady?
>
> Anyone else having had some success with the new EBER probe, reagents
> and software, feel free to comment.  Of the 3 specimens I've run, known
> positive soft tissue, bm bx and cell block, the soft tissue and bm bx
> had some positivity with the EBER DNP and U6 DNP but the cell block was
> negative with both.  There was also lots of blue smearing and haze over
> the slides.  I'm afraid this optimization/validation could end up being
> very expensive even with our 3 control tissues picked up together on
> single slides.
>
> Thanks for everyone's help,
>
> Linda
>
>
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From JHowery2 <@t> yrmc.org  Mon Jul 25 11:58:46 2011
From: JHowery2 <@t> yrmc.org (Howery, Jeffrey)
Date: Mon Jul 25 11:58:50 2011
Subject: [Histonet] Job opening Prescott Arizona
Message-ID: 

Just wanted to add a few details.
We are currently searching for a Cana date for our opening. We are
located in Northern Arizona in the pines. We have two Campuses with the
main campus in Prescott with the second campus located in Prescott
Valley. We are a total of Approx. 187 beds combined. Please Check out
our web site at YRMC.org to see our job opening and information about
our Hospital. Prescott Az on the web to check out the area Arizona is
full of history. We are located 1 1/2 hours north of Phoenix and the
same to Flagstaff. Sedona is about 45 mins away and the Grand Canyon 3
hours. Contact me here  for any questions and if interested fill out an
application on the YRMC web site.  Thanks for your time. Jeff

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From anonwums1 <@t> gmail.com  Mon Jul 25 12:13:32 2011
From: anonwums1 <@t> gmail.com (Adam .)
Date: Mon Jul 25 12:13:37 2011
Subject: [Histonet] Cutting frozen decalcified mouse bones
Message-ID: 

Hi all,

I had a very difficult time cutting my very precious triple transgenic
frozen decalcified mouse bones this morning. As I cut into them, the
sections scrunched up into a mess of OCT pretty much as soon as the blades
hit the block. These were 4% PFA perfused and post-fixed for 24 hours,
decalcified with 14% EDTA using weight loss/weight gain as an endpoint,
cryoprotected overnight in 30% sucrose, embedded in OCT using liquid
nitrogen cooled isopentane vertically (i.e. I was cutting from one growth
plate to the other), and cut at 7 uM. Changing to a new blade didn't help.
Changing the cutting temperature from -10C to -15C didn't help. Changing the
cutting speed didn't help. I can't change the cutting angle without a
special tool, and this isn't my device so I don't want to mess with it.

The only way I could get reasonable sections was to use a tape transfer
system, which seemed like overkill to me, but I need these sections ASAP.
Ideas? I have a whole bunch more bones I need to cut over the next few
weeks...

Thanks,
Adam
From cbrya <@t> lexclin.com  Mon Jul 25 12:42:25 2011
From: cbrya <@t> lexclin.com (Carol Bryant)
Date: Mon Jul 25 12:42:30 2011
Subject: [Histonet] blurry tissue 
Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36F8E@EXCHANGESB>

I would like some thoughts on how to resolve some "blurry" looking tissue.  We have had occasional tissue that looks blurry and not crisp for several weeks now.  It is not all the cases only random tissues.  The tissue is not on the same tissue processor either. We have 2 processors.  The latest cases were a breast, some skins, and a prostate.  I am not certain if this is happening on the tissue processor or in the stainer.  It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene.  It is so hit and miss that I am puzzled.   Any suggestions would be greatly appreciated.
Thank you in advance for your thoughts.

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya@lexclin.com



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This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.  If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.  Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. 
From anna_hughes <@t> merck.com  Mon Jul 25 12:55:58 2011
From: anna_hughes <@t> merck.com (Hughes, Anna)
Date: Mon Jul 25 12:56:05 2011
Subject: [Histonet] Biogenex Xmatrx Infinity Stainer
Message-ID: <0B49B3779F179945B48D29E7793B2A03752AE4613C@USCTMXP51014.merck.com>

Hi Everyone!

I was wondering if anyone out there had and was using the Biogenex Xmatrx Infinity Stainer from Biogenex.  We are considering buying one to do ISH and FISH on, but was wondering what other people had to say about it.   Any feedback would be greatly appreciated!

Thanks!
Anna Hughes

Merck & Co., Inc.
West Point, PA
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From mtitford <@t> aol.com  Mon Jul 25 12:59:24 2011
From: mtitford <@t> aol.com (mtitford@aol.com)
Date: Mon Jul 25 12:59:28 2011
Subject: [Histonet] Formalin down the drain??
Message-ID: <8CE190BB8BCD4CB-1570-244A@Webmail-d105.sysops.aol.com>



I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict.

I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover!

Michael Titford
Pathology USA
Mobile AL USA

From marktarango <@t> gmail.com  Mon Jul 25 13:33:57 2011
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Mon Jul 25 13:34:16 2011
Subject: [Histonet] Antibody suggestions for dog and cat
Message-ID: 

Could anyone suggest suituble antibodies for the following markers in dog
and cat tissue:

Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII

Also could use a suggestion for C-kit in Dog tissue only.

thanks

Mark
From JWeems <@t> sjha.org  Mon Jul 25 13:45:39 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Mon Jul 25 13:45:45 2011
Subject: [Histonet] Cerner CoPath Plus
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082122ADB3@CHEXCMS10.one.ads.che.org>

Anyone using this system, would you please contact me off line? I have questions regarding the PicsPlus component as well as I'm curious to see how everyone likes it.

Thanks! j


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


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From shive003 <@t> umn.edu  Mon Jul 25 13:48:01 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Mon Jul 25 13:48:09 2011
Subject: [Histonet] Antibody suggestions for dog and cat
References: 
Message-ID: <35AF17479BD14C37A9480B70F01A6F75@auxs.umn.edu>

Hi Mark,

Vimentin - Dako; M0725, clone V9
Pankeratin - Dako; M0821; clone MNF116
CD3 - LabVision; RB-9039; rabbit polyclonal
CD20 - LabVision; RB-9013; rabbit polyclonal
CD18 (canine) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); 
clone CA16.3C10
CD18 (feline) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); 
clone FE3.9F2
Melan A - Dako; M7195; clone A103
Factor VIII - Dako; A0082; rabbit polyclonal

CD117 - Dako; A4502; rabbit polyclonal (not specific for dogs; tested 
successfully on cow and horse, also)

Let me know if you need information on any other antibodies in question.  I 
species-validate all of my  IHC tests (100+).

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003@umn.edu

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----- Original Message ----- 
From: "Mark Tarango" 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Monday, July 25, 2011 1:33 PM
Subject: [Histonet] Antibody suggestions for dog and cat


> Could anyone suggest suituble antibodies for the following markers in dog
> and cat tissue:
>
> Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII
>
> Also could use a suggestion for C-kit in Dog tissue only.
>
> thanks
>
> Mark
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From Nacaela.Johnson <@t> USONCOLOGY.COM  Mon Jul 25 13:47:33 2011
From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela)
Date: Mon Jul 25 13:48:37 2011
Subject: [Histonet] blurry tissue 
In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36F8E@EXCHANGESB>
References: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36F8E@EXCHANGESB>
Message-ID: <71882EED22A283429E8424513A22922D595316@txhous1eb015.uson.usoncology.int>

What is the staining intensity like? 


Thanks,
 
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson@USOncology.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Monday, July 25, 2011 12:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] blurry tissue 

I would like some thoughts on how to resolve some "blurry" looking
tissue.  We have had occasional tissue that looks blurry and not crisp
for several weeks now.  It is not all the cases only random tissues.
The tissue is not on the same tissue processor either. We have 2
processors.  The latest cases were a breast, some skins, and a prostate.
I am not certain if this is happening on the tissue processor or in the
stainer.  It has been very humid in our lab so I have started running a
dehumidifier in case there is water in the xylene.  It is so hit and
miss that I am puzzled.   Any suggestions would be greatly appreciated.
Thank you in advance for your thoughts.

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya@lexclin.com



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_______________________________________________
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The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From Kjones <@t> upei.ca Mon Jul 25 13:54:39 2011 From: Kjones <@t> upei.ca (Kathleen Jones) Date: Mon Jul 25 13:54:48 2011 Subject: [Histonet] Antibody suggestions for dog and cat In-Reply-To: References: Message-ID: <4E2D91BF0200008B00009958@grpwise.novell.upei.ca> Hello Mark We use the Ventana Benchmark for most of our IHC. We don't use all of the markers you listed, but and for our canine and feline tissue we do have great results with the following: Vimentin-Ventana Cytokeratin-Dako CD18-UC-Davis (Dr. Peter Moore) C-kit-Dako We worked hard to get the Melan A (Dako) working but had trouble with using a DAB detection kit..perhaps a red kit would be better. If you would like more info, feel free to contact me directly. Kathy Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)566-0595 >>> Mark Tarango 25/07/2011 3:33 PM >>> Could anyone suggest suituble antibodies for the following markers in dog and cat tissue: Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII Also could use a suggestion for C-kit in Dog tissue only. thanks Mark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jarnold <@t> gmi-inc.com Mon Jul 25 14:07:18 2011 From: Jarnold <@t> gmi-inc.com (Jon Arnold) Date: Mon Jul 25 14:07:25 2011 Subject: [Histonet] Looking for assistance in Southeast part of the country- Message-ID: Hello- Is there anyone in the southeast part of the country with familiarity with the: Shandon Hypercenter XP tissue processor? Leica ST5050 IHC stainer (Jung Immunostainer)? I was looking for someone with experience with either of these that may be interested and able to pass along some knowledge to 'new' users of the instruments. Thank you in advance- Regards- Jon Arnold T: 1-800-745-2710 | F: 763-712-8724 From trathborne <@t> somerset-healthcare.com Mon Jul 25 13:18:53 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Jul 25 14:09:42 2011 Subject: [Histonet] Formalin down the drain?? In-Reply-To: <8CE190BB8BCD4CB-1570-244A@Webmail-d105.sysops.aol.com> References: <8CE190BB8BCD4CB-1570-244A@Webmail-d105.sysops.aol.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F426FC@smcmail02.somerset-healthcare.com> Michael, Since this seems to come up somewhat regularly, if you do have a link to the federal laws which govern this, maybe you could share. I'm sure this would help labs get the support they need for proper disposal. Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, July 25, 2011 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From dunatrsd <@t> sbcglobal.net Mon Jul 25 14:15:46 2011 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Mon Jul 25 14:15:49 2011 Subject: [Histonet] Need a protocol for CD24 and CD133 In-Reply-To: <1311125611.65337.YahooMailClassic@web39410.mail.mud.yahoo.com> References: <1311125611.65337.YahooMailClassic@web39410.mail.mud.yahoo.com> Message-ID: <1311621346.37211.YahooMailRC@web83902.mail.sp1.yahoo.com> Hi. Does anyone out there have a protocol for CD24 and CD133 that will work on mouse Xenograft tumors? Preferably a protocol that will work on Leica Bond, but any working protocol will do at this point. Thank you and have a good day. Dusko From dmccaig <@t> ckha.on.ca Mon Jul 25 14:45:21 2011 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Mon Jul 25 14:45:30 2011 Subject: [Histonet] Biocare decloaker Message-ID: We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana From billodonnell <@t> catholichealth.net Mon Jul 25 15:19:52 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Mon Jul 25 15:20:05 2011 Subject: [Histonet] Formalin down the drain?? In-Reply-To: <8CE190BB8BCD4CB-1570-244A@Webmail-d105.sysops.aol.com> References: <8CE190BB8BCD4CB-1570-244A@Webmail-d105.sysops.aol.com> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939513083B@chimsx08.CHI.catholichealth.net> One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain..... you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to.... Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot "Like" this rant on Facebook or follow this rant on Twitter. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Jul 25 15:23:56 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jul 25 15:24:01 2011 Subject: [Histonet] Formalin down the drain?? In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF939513083B@chimsx08.CHI.catholichealth.net> References: <8CE190BB8BCD4CB-1570-244A@Webmail-d105.sysops.aol.com> <4940DF6D1C5FDF48931B6966AAEF939513083B@chimsx08.CHI.catholichealth.net> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EC15A3998@IBMB7Exchange.digestivespecialists.com> I may not be able to "Like" Bill's rant, but I second it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, July 25, 2011 4:20 PM To: mtitford@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain..... you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to.... Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot "Like" this rant on Facebook or follow this rant on Twitter. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Mon Jul 25 15:25:35 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Jul 25 15:25:39 2011 Subject: [Histonet] Where Can I Buy Pneumo Control Slides Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA261E27A9C4@NADCWPMSGCMS03.hca.corpad.net> Good Afternoon to All, I am getting pretty desperate to find a source for Pneumo control slides. I know they are hard to come by, but my favorite source Newcomer Supply does not have any and I ordered some from Sigma-Aldrich and now they are discontinued. Please Help!!!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From mpence <@t> grhs.net Mon Jul 25 15:34:42 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jul 25 15:35:03 2011 Subject: [Histonet] Biocare decloaker In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974C3C@is-e2k3.grhs.net> There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Mon Jul 25 15:40:18 2011 From: chapcl <@t> yahoo.com (William) Date: Mon Jul 25 15:40:29 2011 Subject: [Histonet] Biocare decloaker In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974C3C@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974C3C@is-e2k3.grhs.net> Message-ID: <4F8CD583-C4F4-4D53-9B29-2135D7201B49@yahoo.com> As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, "Mike Pence" wrote: > There is a huge difference between 122 degrees for 30 seconds and 90 > degrees for 45 minutes. I would say you need to look at something here! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana > McCaig > Sent: Monday, July 25, 2011 2:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biocare decloaker > > > We are trying to validate a Biocare decloaker and have found when we use > 122 degrees for 30 seconds we get great signal, but distorted > morphology. > > > > If we reduce to 90 degrees for 45 minutes, the signal is significantly > decreased but the morphology is good. > > > > What protocols are you using? > > > > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Mon Jul 25 15:42:08 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Jul 25 15:42:14 2011 Subject: [Histonet] Biocare decloaker In-Reply-To: <4F8CD583-C4F4-4D53-9B29-2135D7201B49@yahoo.com> References: <661949901A768E4F9CC16D8AF8F2838C03974C3C@is-e2k3.grhs.net> <4F8CD583-C4F4-4D53-9B29-2135D7201B49@yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082122AE0A@CHEXCMS10.one.ads.che.org> I wonder if a new gasket would help me.. I keep blowing mine! :>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William Sent: Monday, July 25, 2011 16:40 To: Mike Pence Cc: ; Diana McCaig Subject: Re: [Histonet] Biocare decloaker As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, "Mike Pence" wrote: > There is a huge difference between 122 degrees for 30 seconds and 90 > degrees for 45 minutes. I would say you need to look at something here! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana > McCaig > Sent: Monday, July 25, 2011 2:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biocare decloaker > > > We are trying to validate a Biocare decloaker and have found when we > use > 122 degrees for 30 seconds we get great signal, but distorted > morphology. > > > > If we reduce to 90 degrees for 45 minutes, the signal is > significantly decreased but the morphology is good. > > > > What protocols are you using? > > > > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rjbuesa <@t> yahoo.com Mon Jul 25 15:45:42 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 25 15:45:46 2011 Subject: [Histonet] blurry tissue In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36F8E@EXCHANGESB> Message-ID: <1311626742.62013.YahooMailClassic@web65703.mail.ac4.yahoo.com> You just pointed out to the most likely causes: happening during staining, high humidity and water in the xylene. Try to take care of these issues and you will resolve the problem. Ren? J. --- On Mon, 7/25/11, Carol Bryant wrote: From: Carol Bryant Subject: [Histonet] blurry tissue To: "histonet@lists.utsouthwestern.edu" Date: Monday, July 25, 2011, 1:42 PM I would like some thoughts on how to resolve some "blurry" looking tissue.? We have had occasional tissue that looks blurry and not crisp for several weeks now.? It is not all the cases only random tissues.? The tissue is not on the same tissue processor either. We have 2 processors.? The latest cases were a breast, some skins, and a prostate.? I am not certain if this is happening on the tissue processor or in the stainer.? It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene.? It is so hit and miss that I am puzzled.???Any suggestions would be greatly appreciated. Thank you in advance for your thoughts. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.? If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.? Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Mon Jul 25 15:45:12 2011 From: chapcl <@t> yahoo.com (William) Date: Mon Jul 25 15:47:11 2011 Subject: [Histonet] Biocare decloaker In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164082122AE0A@CHEXCMS10.one.ads.che.org> References: <661949901A768E4F9CC16D8AF8F2838C03974C3C@is-e2k3.grhs.net> <4F8CD583-C4F4-4D53-9B29-2135D7201B49@yahoo.com> <92AD9B20A6C38C4587A9FEBE3A30E164082122AE0A@CHEXCMS10.one.ads.che.org> Message-ID: <5E4B798A-0217-4041-916E-8703D61034C2@yahoo.com> Ok that is a bigger problem and a very clear answer to your staining issue. Your decloaker should never "blow" a gasket. Something is wrong and you probably need service or a replacement. Your decloaker is probably getting too hot and over retrieving tissue. I suggest immediately calling Biocare technical support. You need this fixed! Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:42 PM, "Weems, Joyce" wrote: > I wonder if a new gasket would help me.. I keep blowing mine! :>) > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William > Sent: Monday, July 25, 2011 16:40 > To: Mike Pence > Cc: ; Diana McCaig > Subject: Re: [Histonet] Biocare decloaker > > As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. > > My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. > > Will Chappell > > Sent from my iPhone > > On Jul 25, 2011, at 1:34 PM, "Mike Pence" wrote: > >> There is a huge difference between 122 degrees for 30 seconds and 90 >> degrees for 45 minutes. I would say you need to look at something here! >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana >> McCaig >> Sent: Monday, July 25, 2011 2:45 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Biocare decloaker >> >> >> We are trying to validate a Biocare decloaker and have found when we >> use >> 122 degrees for 30 seconds we get great signal, but distorted >> morphology. >> >> >> >> If we reduce to 90 degrees for 45 minutes, the signal is >> significantly decreased but the morphology is good. >> >> >> >> What protocols are you using? >> >> >> >> Diana >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > From rjbuesa <@t> yahoo.com Mon Jul 25 15:53:54 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 25 15:53:58 2011 Subject: [Histonet] Formalin down the drain?? In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF939513083B@chimsx08.CHI.catholichealth.net> Message-ID: <1311627234.95809.YahooMailClassic@web65704.mail.ac4.yahoo.com> Your "rant" is interesting but wrong. OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous materials down the drain. I was also taken aback by Amy's posting. No, regardless of what your state law may or may not permit you to dump in the drain, you should not put some $avings over the well being of the environment and the drinking water of people. Formaldehyde is toxic and recently officially declared carcinogen. In the same way that "frackting" methods to obtain gas from shale has been deemed dangerous, equally dumping formaldehyde, xylene and any other chemical ought to be the source of concern. This in my "rant"! Ren? J. --- On Mon, 7/25/11, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: RE: [Histonet] Formalin down the drain?? To: mtitford@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 4:19 PM One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain..... you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to.... Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :)? You cannot "Like" this rant on Facebook or follow this rant on Twitter. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Mon Jul 25 15:56:05 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Jul 25 15:56:17 2011 Subject: [Histonet] Formalin down the drain?? In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF939513083B@chimsx08.CHI.catholichealth.net> References: <8CE190BB8BCD4CB-1570-244A@Webmail-d105.sysops.aol.com> <4940DF6D1C5FDF48931B6966AAEF939513083B@chimsx08.CHI.catholichealth.net> Message-ID: <493CAA64F203E14E8823737B9EE0E25F08FE8409C4@EXCHMB01.isis.local> I totally agree, under any circumstances, whatever the State law defines, we should not dump formalin, xylene or alcohols down the drain. And even though California has very strict rules, it even seems to differ from county to county. In one of my past jobs we were allowed to dump the formalin down the drain (Orange County of all places), whereas in San Diego, that was absolutely prohibited. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, July 25, 2011 1:20 PM To: mtitford@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain..... you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to.... Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot "Like" this rant on Facebook or follow this rant on Twitter. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Mon Jul 25 16:02:58 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Jul 25 16:03:02 2011 Subject: [Histonet] Formalin down the drain?? In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F08FE8409C4@EXCHMB01.isis.local> References: <8CE190BB8BCD4CB-1570-244A@Webmail-d105.sysops.aol.com> <4940DF6D1C5FDF48931B6966AAEF939513083B@chimsx08.CHI.catholichealth.net> <493CAA64F203E14E8823737B9EE0E25F08FE8409C4@EXCHMB01.isis.local> Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106D2E66@hc-mailboxc1-n3.healthcare.uiowa.edu> Indeed not only county to county but your local municipality can have specific regulations related to hazardous waste discard in the sewer since it is their waste treatment plant that takes the hit so to speak. Most small municipalities will adopt state or county regulations (whichever is stricter) but they can always write even stricter rules if they wish and that may well be the case in some larger metro areas. Good luck. Joe University of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Monday, July 25, 2011 3:56 PM To: 'O'Donnell, Bill'; mtitford@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? I totally agree, under any circumstances, whatever the State law defines, we should not dump formalin, xylene or alcohols down the drain. And even though California has very strict rules, it even seems to differ from county to county. In one of my past jobs we were allowed to dump the formalin down the drain (Orange County of all places), whereas in San Diego, that was absolutely prohibited. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, July 25, 2011 1:20 PM To: mtitford@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain..... you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to.... Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot "Like" this rant on Facebook or follow this rant on Twitter. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From mwich <@t> 7thwavelabs.com Mon Jul 25 16:03:19 2011 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Mon Jul 25 16:03:23 2011 Subject: [Histonet] Where Can I Buy Pneumo Control Slides References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA261E27A9C4@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <62A8156F8071C8439080D626DF8C33A601910D41@wave-mail.7thwave.local> American MasterTech sells them: http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&ite m=CSP015P -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Monday, July 25, 2011 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where Can I Buy Pneumo Control Slides Good Afternoon to All, I am getting pretty desperate to find a source for Pneumo control slides. I know they are hard to come by, but my favorite source Newcomer Supply does not have any and I ordered some from Sigma-Aldrich and now they are discontinued. Please Help!!!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Mon Jul 25 16:19:46 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon Jul 25 16:19:51 2011 Subject: [Histonet] RE: Histonet Digest, Vol 92, Issue 33 In-Reply-To: <20110725205712.E50B18B7051@mx10.myoutlookonline.com> References: <20110725205712.E50B18B7051@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E3786E8F64@S10MAILD001N1.SH10.lan> This sounds to me like a processing issue, especially with the types of tissues you describe. Are the tissue sections you are referring to very thick? Maybe the tissue is too big for the amount of time in the different processing stations. I have also seen this happen when the tissue has been left to dry out before the clinician puts it into the formalin. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 2 Date: Mon, 25 Jul 2011 13:42:25 -0400 From: Carol Bryant Subject: [Histonet] blurry tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36F8E@EXCHANGESB> Content-Type: text/plain; charset="us-ascii" I would like some thoughts on how to resolve some "blurry" looking tissue. We have had occasional tissue that looks blurry and not crisp for several weeks now. It is not all the cases only random tissues. The tissue is not on the same tissue processor either. We have 2 processors. The latest cases were a breast, some skins, and a prostate. I am not certain if this is happening on the tissue processor or in the stainer. It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene. It is so hit and miss that I am puzzled. Any suggestions would be greatly appreciated. Thank you in advance for your thoughts. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ------------------------------ From laurie.colbert <@t> huntingtonhospital.com Mon Jul 25 16:21:36 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Jul 25 16:21:40 2011 Subject: [Histonet] Where Can I Buy Pneumo Control Slides In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA261E27A9C4@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA261E27A9C4@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AEDA@EXCHANGE3.huntingtonhospital.com> American Master Tech. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Monday, July 25, 2011 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where Can I Buy Pneumo Control Slides Good Afternoon to All, I am getting pretty desperate to find a source for Pneumo control slides. I know they are hard to come by, but my favorite source Newcomer Supply does not have any and I ordered some from Sigma-Aldrich and now they are discontinued. Please Help!!!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Mon Jul 25 16:59:32 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Mon Jul 25 16:59:36 2011 Subject: [Histonet] Where Can I Buy Pneumo Control Slides In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2AEDA@EXCHANGE3.huntingtonhospital.com> Message-ID: Statlab sells pneumo controls and they are in human tissues. Thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com ----- Original Message ----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Monday, July 25, 2011 04:21 PM To: Wanda.Smith@HCAhealthcare.com ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Where Can I Buy Pneumo Control Slides American Master Tech. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Monday, July 25, 2011 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where Can I Buy Pneumo Control Slides Good Afternoon to All, I am getting pretty desperate to find a source for Pneumo control slides. I know they are hard to come by, but my favorite source Newcomer Supply does not have any and I ordered some from Sigma-Aldrich and now they are discontinued. Please Help!!!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Mon Jul 25 19:27:31 2011 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Mon Jul 25 19:47:10 2011 Subject: [Histonet] H&E protocol question In-Reply-To: <8DDCDA33-B24D-498E-AA0B-6DCB4B436439@imagesbyhopper.com> References: <8DDCDA33-B24D-498E-AA0B-6DCB4B436439@imagesbyhopper.com> Message-ID: <4E2E9691.411C.0039.1@health.qld.gov.au> Hi Michelle I have used both and my experience either is suitable for a good H&E if they are used appropriately. For regressive staining you just need to optimise the time of haematoxylin staining with the concentration and time of the differentiation step. In a previous position I used Mayer's which is a progressive stain. We stained for 4 minutes in our H&E protocol however to get the best staining we gave it a "whiff" of differentiation (1 min with 0.025% Acid alcohol) This was not to differentiate the nuclear staining but we found to get optimal nuclear staining the time required would result in some tissue types adopting a very pale blue haze in the background and removing this provided much better differential eosin staining. For counterstaining special stains and IHC the Mayer's was by far the best I have used. 1 minute would provide delicate nuclear staining with a clear background. This stain would not interfere with any other staining including nuclear staining for ER, PR, TTF1 etc. Good bluing was achieved simply with a good wash in our quite basic tap water. So I would recommend either for H&E but progressive for a counterstain. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "histotech@imagesbyhopper.com" 7/25/2011 9:29 pm >>> Hi Histonetters! I'm looking for thoughts on preferences/pros/cons between using a progressive and a regressive H&E on routine daily work. Which hematoxylins do you prefer (commercially prepared), which eosin? Anyone have a tried and true protocol for each method? Thanks! Michelle Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From hlukey <@t> msn.com Mon Jul 25 21:53:29 2011 From: hlukey <@t> msn.com (Hugh Luk) Date: Mon Jul 25 21:53:33 2011 Subject: [Histonet] How hot is too hot for storing tissue blocks? Message-ID: Hi folks, We were hoping someone could recommend a temperature range acceptable for storing tissue blocks? Also do you folks use a regular thermometer or a temperature chart recorder (records on graph) to record room temps? We are going to move to a new location and need help with the fine details. Thanks in advance, Hugh Hawaii From wilsonjonz <@t> yahoo.com Mon Jul 25 23:50:04 2011 From: wilsonjonz <@t> yahoo.com (Ruthie Wilson) Date: Mon Jul 25 23:50:10 2011 Subject: [Histonet] (no subject) Message-ID: <1311655804.62783.YahooMailClassic@web113914.mail.gq1.yahoo.com> Please unsubscribe me From histotech <@t> imagesbyhopper.com Tue Jul 26 05:07:57 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Jul 26 05:08:02 2011 Subject: [Histonet] (no subject) In-Reply-To: <1311655804.62783.YahooMailClassic@web113914.mail.gq1.yahoo.com> References: <1311655804.62783.YahooMailClassic@web113914.mail.gq1.yahoo.com> Message-ID: <92232EA0-F177-43A4-A2F4-4CA2670F9045@imagesbyhopper.com> Ruthie, In order to unsubscribe from the list, you must click on the link at the bottom of the email, scroll to the bottom of the webpage and enter your email requesting to be unsubscribed. You have the power to unsubscribe yourself! Good Luck! Michelle Sent from my iPhone On Jul 26, 2011, at 12:50 AM, Ruthie Wilson wrote: > Please unsubscribe me > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DKBoyd <@t> chs.net Tue Jul 26 07:35:38 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Jul 26 07:36:54 2011 Subject: [Histonet] Formalin down the drain?? In-Reply-To: <1311627234.95809.YahooMailClassic@web65704.mail.ac4.yahoo.com> Message-ID: Ditto Rene! All Federal Regulations "trump" state if they are more stringent. In other words states can have more stringent regulations but NEVER less. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 07/25/2011 04:54 PM To mtitford@aol.com, histonet@lists.utsouthwestern.edu, BillO'Donnell cc Subject RE: [Histonet] Formalin down the drain?? Your "rant" is interesting but wrong. OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous materials down the drain. I was also taken aback by Amy's posting. No, regardless of what your state law may or may not permit you to dump in the drain, you should not put some $avings over the well being of the environment and the drinking water of people. Formaldehyde is toxic and recently officially declared carcinogen. In the same way that "frackting" methods to obtain gas from shale has been deemed dangerous, equally dumping formaldehyde, xylene and any other chemical ought to be the source of concern. This in my "rant"! Ren? J. --- On Mon, 7/25/11, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: RE: [Histonet] Formalin down the drain?? To: mtitford@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 4:19 PM One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain..... you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to.... Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot "Like" this rant on Facebook or follow this rant on Twitter. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From brian <@t> prometheushealthcare.com Tue Jul 26 07:59:07 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Tue Jul 26 07:59:18 2011 Subject: [Histonet] Histologist Opening in Naples FL Message-ID: <005f01cc4b93$d1a99790$74fcc6b0$@com> New Opening with a hospital in Naples Day shift position hours roughly 6am to 2:30pm Prefer HT/HTL (ASCP) Will be cutting, staining Please contact me today for immediate consideration Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From TGoins <@t> mt.gov Tue Jul 26 08:27:12 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Jul 26 08:27:36 2011 Subject: [Histonet] Antibody suggestions for dog and cat In-Reply-To: References: Message-ID: Hi Mark - We have had good results with: Vimentin Clone V9 (CM048), Factor VIII (PAb-CP039) and pan-Cytokeratin, Clone Lu-5 (CM043) from Biocare CD3 C7930 from Sigma CD79a Clone HM47/A9 (MS357), Melan A (MS799) from Thermo Scientific - no luck with CD20. CD117 (RB9038) from Thermo Scientific - data sheet states heat retrieval is required, but we get good results without retrieval. I have also found that while Melan A is excellent in canine tissue, S100 (PA1-38584, ThermoSci) works better in feline tissue - more positive hits with higher intensity of specific staining. Best regards, Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Monday, July 25, 2011 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody suggestions for dog and cat Could anyone suggest suituble antibodies for the following markers in dog and cat tissue: Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII Also could use a suggestion for C-kit in Dog tissue only. thanks Mark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Tue Jul 26 08:31:49 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Jul 26 08:32:01 2011 Subject: [Histonet] blurry tissue In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36F8E@EXCHANGESB> Message-ID: Are you using recycled alcohols? Check your concentrations. Could your cassettes be "floating up" during processing? It is possible they have air bubbles trapped in the cassette and aren't properly dehydrated. Check and change your staining alcohols and clearants to make sure there isn't water from humidity or carry over. It has always been my experience that recycled dehydrants/clearants are the culprit. But, that being said, we have on occasions had an errant cassettes to float up during processing. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Carol Bryant Sent by: histonet-bounces@lists.utsouthwestern.edu 07/25/2011 01:42 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] blurry tissue I would like some thoughts on how to resolve some "blurry" looking tissue. We have had occasional tissue that looks blurry and not crisp for several weeks now. It is not all the cases only random tissues. The tissue is not on the same tissue processor either. We have 2 processors. The latest cases were a breast, some skins, and a prostate. I am not certain if this is happening on the tissue processor or in the stainer. It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene. It is so hit and miss that I am puzzled. Any suggestions would be greatly appreciated. Thank you in advance for your thoughts. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From shive003 <@t> umn.edu Tue Jul 26 08:48:40 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Jul 26 08:48:43 2011 Subject: [Histonet] Antibody suggestions for dog and cat Message-ID: <589A2BD674CC41D5B9FD890DF46A43F2@auxs.umn.edu> Correction: I just found out that LabVision no longer sells the CD3 catalog number that I listed below; we also use CD3 catalog # RB-360 equally well on all species. Jan Shivers UMN VDL ----- Original Message ----- From: "Jan Shivers" To: "Mark Tarango" ; "histonet@lists.utsouthwestern.edu" Sent: Monday, July 25, 2011 1:48 PM Subject: Re: [Histonet] Antibody suggestions for dog and cat > Hi Mark, > > Vimentin - Dako; M0725, clone V9 > Pankeratin - Dako; M0821; clone MNF116 > CD3 - LabVision; RB-9039; rabbit polyclonal > CD20 - LabVision; RB-9013; rabbit polyclonal > CD18 (canine) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); > clone CA16.3C10 > CD18 (feline) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); > clone FE3.9F2 > Melan A - Dako; M7195; clone A103 > Factor VIII - Dako; A0082; rabbit polyclonal > > CD117 - Dako; A4502; rabbit polyclonal (not specific for dogs; tested > successfully on cow and horse, also) > > Let me know if you need information on any other antibodies in question. > I species-validate all of my IHC tests (100+). > > Jan Shivers > Senior Scientist > Histology/IHC/EM Section Head > Pathology Teaching Program > University of Minnesota > Veterinary Diagnostic Laboratory > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > > (Confidentiality Notice: This message, together with any attachments, is > intended only for the use of the individual or entity to which it is > addressed and may contain confidential or privileged information. If you > think you have received this message in error, please advise the sender > and then delete this message and any attachments immediately.) > > > ----- Original Message ----- > From: "Mark Tarango" > To: "histonet@lists.utsouthwestern.edu" > > Sent: Monday, July 25, 2011 1:33 PM > Subject: [Histonet] Antibody suggestions for dog and cat > > >> Could anyone suggest suituble antibodies for the following markers in dog >> and cat tissue: >> >> Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII >> >> Also could use a suggestion for C-kit in Dog tissue only. >> >> thanks >> >> Mark >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From ASelf <@t> georgetownhospitalsystem.org Tue Jul 26 09:16:09 2011 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue Jul 26 09:16:15 2011 Subject: [Histonet] (no subject) Message-ID: Good Morning All, We do the non-gyn cytology cases in the histology lab and usually I will stain the body fluids separate. Now that our workload has increased staining these specimens separately takes up a lot of time. I was wandering what other labs were doing to prevent cross-contamination highly cellular specimens during processing and staining. I am referring to the CAP question below: CYP.07680 ? Are there procedures to prevent cross-contamination of specimens during processing and staining? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From Bauer.Karen <@t> mayo.edu Tue Jul 26 09:38:54 2011 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Jul 26 09:39:10 2011 Subject: [Histonet] MOHS daily flow... Message-ID: <53FC421CC200C5429929EDE6C3676F300180813B@msgebe34> Hello, This is for those techs that assist in the MOHS lab... What is your daily flow for the work performed in your lab? How are patients usually scheduled? (Are they staggered? All come in at approximately the same time so 1st layers can be taken right away?) How often are you receiving patient specimens? Usually, how many patients per day? Do you gross the tissue before freezing? What is the goal time for slide completion? (From the time the tissue is placed in the cryostat to freeze to the slide being ready to stain.) How many levels are you placing on the slide? How many slides per block are you cutting? Do you have a set time limit on when all first layers are to be done? Any additional information is greatly appreciated. Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.karen@mayo.edu ___________________________________________ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org From rjbuesa <@t> yahoo.com Tue Jul 26 09:50:56 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 26 09:51:05 2011 Subject: [Histonet] How hot is too hot for storing tissue blocks? In-Reply-To: Message-ID: <1311691856.56191.YahooMailClassic@web65714.mail.ac4.yahoo.com> 80?F the most. Use a thermometer and check the temperature weekly. Ren? J. --- On Mon, 7/25/11, Hugh Luk wrote: From: Hugh Luk Subject: [Histonet] How hot is too hot for storing tissue blocks? To: histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 10:53 PM Hi folks, We were hoping someone could recommend a temperature range acceptable for storing tissue blocks?? Also do you folks use a regular thermometer or a temperature chart recorder (records on graph) to record room temps? We are going to move to a new location and need help with the fine details. Thanks in advance, Hugh Hawaii ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Jul 26 10:15:03 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Jul 26 10:15:12 2011 Subject: [Histonet] Formalin down the drain?? In-Reply-To: <1311627234.95809.YahooMailClassic@web65704.mail.ac4.yahoo.com> References: <4940DF6D1C5FDF48931B6966AAEF939513083B@chimsx08.CHI.catholichealth.net> <1311627234.95809.YahooMailClassic@web65704.mail.ac4.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF04F1C5@LRGHEXVS1.practice.lrgh.org> Not just OSHA, but the EPA prohibits it http://www.epa.gov/lawsregs/topics/waste.html#hazwaste Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 25, 2011 4:54 PM To: mtitford@aol.com; histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: RE: [Histonet] Formalin down the drain?? Your "rant" is interesting but wrong. OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous materials down the drain. I was also taken aback by Amy's posting. No, regardless of what your state law may or may not permit you to dump in the drain, you should not put some $avings over the well being of the environment and the drinking water of people. Formaldehyde is toxic and recently officially declared carcinogen. In the same way that "frackting" methods to obtain gas from shale has been deemed dangerous, equally dumping formaldehyde, xylene and any other chemical ought to be the source of concern. This in my "rant"! Ren? J. --- On Mon, 7/25/11, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: RE: [Histonet] Formalin down the drain?? To: mtitford@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 4:19 PM One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain..... you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to.... Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :)? You cannot "Like" this rant on Facebook or follow this rant on Twitter. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From TJJ <@t> stowers.org Tue Jul 26 10:29:06 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Tue Jul 26 10:29:11 2011 Subject: [Histonet] LR White Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9770566A9@EXCHMB-02.stowers-institute.org> Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our H&E stains are a little bit pale. Thanks in advance! Teri From rsrichmond <@t> gmail.com Tue Jul 26 10:30:59 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Jul 26 10:31:03 2011 Subject: [Histonet] Re: Where Can I Buy Pneumo Control Slides Message-ID: At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's "Pneumo"? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN From oneilb <@t> wvuhealthcare.com Tue Jul 26 10:35:55 2011 From: oneilb <@t> wvuhealthcare.com (Oneil, Beth Ann) Date: Tue Jul 26 10:34:50 2011 Subject: [Histonet] Re: Pneumocystis Control Slides Message-ID: <052C41417E0EF64D8E201FE822D36CA90A1C5A0D@NT-EXCHANGE.wvuh.wvuhs.com> I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sbreeden <@t> nmda.nmsu.edu Tue Jul 26 11:27:24 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jul 26 11:27:29 2011 Subject: [Histonet] Digital pathology systems Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF91D@nmdamailsvr.nmda.ad.nmsu.edu> At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was "attached" to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From Wanda.Smith <@t> HCAhealthcare.com Tue Jul 26 12:07:05 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Tue Jul 26 12:07:12 2011 Subject: [Histonet] Thanks for Control Offers and Suggestions!!! Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA261E355DA1@NADCWPMSGCMS03.hca.corpad.net> Thanks to everyone for your offers and suggestions. I am having a block sent from the control bank at NSH. Thanks again to all, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From bkarim <@t> biocare.net Tue Jul 26 12:36:32 2011 From: bkarim <@t> biocare.net (Brenda Karim) Date: Tue Jul 26 12:36:37 2011 Subject: [Histonet] Antibody suggestions for dog and cat In-Reply-To: References: Message-ID: <78479E7E8229B54F82F87C79C845A7420536D573@sclexc02.insrchosting.com> Mark, Biocare Medical has the following antibodies you are looking for that work on canine tissue (feline not tested): Vimentin - Mouse, clone V9 (CM048) Vimentin - Rabbit, clone SP20 (CRM312) CD3 T-cell - Mouse, clone PS1 (CM110) Mart-1 Cocktail (MelanA) - Mouse, clone M2-7C10+M2-9E3 (CM077) Factor VIII - Rabbit polyclonal (CP039) CD117/c-kit - Rabbit, clone Y145 (CME296) These antibodies work on both canine and feline tissue: Pan Cytokeratin, Mouse, clone AE1/AE3 (CM011) Pan Cytokeratin, Mouse, clone Lu-5 (CM043) Pan Cytokeratin Plus - Mouse, clone AE1/AE3 + 5D3(CM162) CD3 - Rabbit polyclonal (CP215) Feel free to contact me directly if you would like more information or call our Customer Service at 800-799-9499 Opt. #1 Thank you, Brenda Brenda Karim, B.S., M.B.A. Associate Product Manager Biocare Medical, LLC. 4040 Pike Lane, Concord, CA 94520 www.biocare.net tel: 925-603-8072 cell: 925-768-9902 fax: 925-887-8777 email: bkarim@biocare.net From nicole <@t> dlcjax.com Tue Jul 26 12:40:03 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Jul 26 12:40:14 2011 Subject: [Histonet] MOHS daily flow... In-Reply-To: <53FC421CC200C5429929EDE6C3676F300180813B@msgebe34> References: <53FC421CC200C5429929EDE6C3676F300180813B@msgebe34> Message-ID: <1411.208.62.167.196.1311702003.squirrel@webmail.realpages.com> Karen The number of patients depends on the surgeon. Some do 4-15 per day. It also depends on how many rooms you have to see patients. For the most part, Lets say you had two rooms available. and had 6 patients scheduled. schedule pt 15-20 mimutes apart. Bring first two in at same time. Doctor can get layers bandage pateints and place them in a waiting area. Next two patients come in get prepared and layers are taken. Place in waiting area. Next patients are brought in. layers taken. Place in waiting area. Each specmen after layer is taken is brought in lab. Mohs tech should be able to complete each case within 15-20minutes. Slides are placed for doc to read. Once he has taken all layers he can begin to read ready cases and take layers or begin closures. Pateints layers are taken and then they are placed back in waiting areas. This will continue until are patients are done. Tissue is grossed before it is placed in cryo. Two slides should be cut per peice of tissue. Not per case. We place 3 sections on each slide. Each section is a little deeper then the one before. We start at the frosted end. A great fast effecient tech should be able to handle 10-15 cases per day. I hope this helps. Nicole Tatum HT ASCP > > This is for those techs that assist in the MOHS lab... > > What is your daily flow for the work performed in your lab? > How are patients usually scheduled? (Are they staggered? All come in > at approximately the same time so 1st layers can be taken right away?) > How often are you receiving patient specimens? > Usually, how many patients per day? > Do you gross the tissue before freezing? > What is the goal time for slide completion? (From the time the tissue > is placed in the cryostat to freeze to the slide being ready to stain.) > How many levels are you placing on the slide? > How many slides per block are you cutting? > Do you have a set time limit on when all first layers are to be done? > > Any additional information is greatly appreciated. > > Thanks much!! > > Karen > > Karen L. Bauer HTL/HT (ASCP) > Histology Supervisor > Pathology Department > Mayo Clinic Health System in Eau Claire > E-mail: bauer.karen@mayo.edu > ___________________________________________ > Mayo Clinic Health System > 1221 Whipple St. > Eau Claire, WI 54703 > www.mayoclinichealthsystem.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tina.phan <@t> ventana.roche.com Tue Jul 26 12:51:55 2011 From: tina.phan <@t> ventana.roche.com (Phan, Tina) Date: Tue Jul 26 12:52:00 2011 Subject: [Histonet] Re: Histonet Digest, Vol 92, Issue 35 Message-ID: <704E6ADBC5DA1B458DE3EF44CF53D0720100877397@RNUMSEM721.nala.roche.com> Rrr Tina (Phan) McLaughlin SYMPHONY Product Specialist Ventana Medical Systems, a Division of Roche Cell: 520.230.0175 www.ventanamed.com Sent by Verizon Blackberry ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Tue Jul 26 19:02:28 2011 Subject: Histonet Digest, Vol 92, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. LR White (Johnson, Teri) 2. Re: Where Can I Buy Pneumo Control Slides (Bob Richmond) 3. Re: Pneumocystis Control Slides (Oneil, Beth Ann) 4. Digital pathology systems (Breeden, Sara) ---------------------------------------------------------------------- Message: 1 Date: Tue, 26 Jul 2011 10:29:06 -0500 From: "Johnson, Teri" Subject: [Histonet] LR White To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9770566A9@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our H&E stains are a little bit pale. Thanks in advance! Teri ------------------------------ Message: 2 Date: Tue, 26 Jul 2011 11:30:59 -0400 From: Bob Richmond Subject: [Histonet] Re: Where Can I Buy Pneumo Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's "Pneumo"? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Tue, 26 Jul 2011 11:35:55 -0400 From: "Oneil, Beth Ann" Subject: [Histonet] Re: Pneumocystis Control Slides To: Message-ID: <052C41417E0EF64D8E201FE822D36CA90A1C5A0D@NT-EXCHANGE.wvuh.wvuhs.com> Content-Type: text/plain; charset="iso-8859-1" I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 4 Date: Tue, 26 Jul 2011 10:27:24 -0600 From: "Breeden, Sara" Subject: [Histonet] Digital pathology systems To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF91D@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was "attached" to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 35 **************************************** From tina.phan <@t> ventana.roche.com Tue Jul 26 12:52:07 2011 From: tina.phan <@t> ventana.roche.com (Phan, Tina) Date: Tue Jul 26 12:52:13 2011 Subject: [Histonet] Re: Histonet Digest, Vol 92, Issue 35 Message-ID: <704E6ADBC5DA1B458DE3EF44CF53D0720100877398@RNUMSEM721.nala.roche.com> Trtttrtttttrrrr Tina (Phan) McLaughlin SYMPHONY Product Specialist Ventana Medical Systems, a Division of Roche Cell: 520.230.0175 www.ventanamed.com Sent by Verizon Blackberry ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Tue Jul 26 19:02:28 2011 Subject: Histonet Digest, Vol 92, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. LR White (Johnson, Teri) 2. Re: Where Can I Buy Pneumo Control Slides (Bob Richmond) 3. Re: Pneumocystis Control Slides (Oneil, Beth Ann) 4. Digital pathology systems (Breeden, Sara) ---------------------------------------------------------------------- Message: 1 Date: Tue, 26 Jul 2011 10:29:06 -0500 From: "Johnson, Teri" Subject: [Histonet] LR White To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9770566A9@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our H&E stains are a little bit pale. Thanks in advance! Teri ------------------------------ Message: 2 Date: Tue, 26 Jul 2011 11:30:59 -0400 From: Bob Richmond Subject: [Histonet] Re: Where Can I Buy Pneumo Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's "Pneumo"? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Tue, 26 Jul 2011 11:35:55 -0400 From: "Oneil, Beth Ann" Subject: [Histonet] Re: Pneumocystis Control Slides To: Message-ID: <052C41417E0EF64D8E201FE822D36CA90A1C5A0D@NT-EXCHANGE.wvuh.wvuhs.com> Content-Type: text/plain; charset="iso-8859-1" I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 4 Date: Tue, 26 Jul 2011 10:27:24 -0600 From: "Breeden, Sara" Subject: [Histonet] Digital pathology systems To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF91D@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was "attached" to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 35 **************************************** From tina.phan <@t> ventana.roche.com Tue Jul 26 12:52:40 2011 From: tina.phan <@t> ventana.roche.com (Phan, Tina) Date: Tue Jul 26 12:52:45 2011 Subject: [Histonet] Re: Histonet Digest, Vol 92, Issue 35 Message-ID: <704E6ADBC5DA1B458DE3EF44CF53D0720100877399@RNUMSEM721.nala.roche.com> R Tina (Phan) McLaughlin SYMPHONY Product Specialist Ventana Medical Systems, a Division of Roche Cell: 520.230.0175 www.ventanamed.com Sent by Verizon Blackberry ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Tue Jul 26 19:02:28 2011 Subject: Histonet Digest, Vol 92, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. LR White (Johnson, Teri) 2. Re: Where Can I Buy Pneumo Control Slides (Bob Richmond) 3. Re: Pneumocystis Control Slides (Oneil, Beth Ann) 4. Digital pathology systems (Breeden, Sara) ---------------------------------------------------------------------- Message: 1 Date: Tue, 26 Jul 2011 10:29:06 -0500 From: "Johnson, Teri" Subject: [Histonet] LR White To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9770566A9@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our H&E stains are a little bit pale. Thanks in advance! Teri ------------------------------ Message: 2 Date: Tue, 26 Jul 2011 11:30:59 -0400 From: Bob Richmond Subject: [Histonet] Re: Where Can I Buy Pneumo Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's "Pneumo"? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Tue, 26 Jul 2011 11:35:55 -0400 From: "Oneil, Beth Ann" Subject: [Histonet] Re: Pneumocystis Control Slides To: Message-ID: <052C41417E0EF64D8E201FE822D36CA90A1C5A0D@NT-EXCHANGE.wvuh.wvuhs.com> Content-Type: text/plain; charset="iso-8859-1" I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 4 Date: Tue, 26 Jul 2011 10:27:24 -0600 From: "Breeden, Sara" Subject: [Histonet] Digital pathology systems To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF91D@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was "attached" to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 35 **************************************** From jqb7 <@t> cdc.gov Tue Jul 26 12:52:45 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Jul 26 12:52:51 2011 Subject: [Histonet] MOHS daily flow... In-Reply-To: <1411.208.62.167.196.1311702003.squirrel@webmail.realpages.com> References: <53FC421CC200C5429929EDE6C3676F300180813B@msgebe34> <1411.208.62.167.196.1311702003.squirrel@webmail.realpages.com> Message-ID: That is very interesting. A friend had a procedure done last week and they kept him on the table until they were done. he did not go back to a waiting area. Is this common? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, July 26, 2011 1:40 PM To: Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] MOHS daily flow... Importance: Low Karen The number of patients depends on the surgeon. Some do 4-15 per day. It also depends on how many rooms you have to see patients. For the most part, Lets say you had two rooms available. and had 6 patients scheduled. schedule pt 15-20 mimutes apart. Bring first two in at same time. Doctor can get layers bandage pateints and place them in a waiting area. Next two patients come in get prepared and layers are taken. Place in waiting area. Next patients are brought in. layers taken. Place in waiting area. Each specmen after layer is taken is brought in lab. Mohs tech should be able to complete each case within 15-20minutes. Slides are placed for doc to read. Once he has taken all layers he can begin to read ready cases and take layers or begin closures. Pateints layers are taken and then they are placed back in waiting areas. This will continue until are patients are done. Tissue is grossed before it is placed in cryo. Two slides should be cut per peice of tissue. Not per case. We place 3 sections on each slide. Each section is a little deeper then the one before. We start at the frosted end. A great fast effecient tech should be able to handle 10-15 cases per day. I hope this helps. Nicole Tatum HT ASCP > > This is for those techs that assist in the MOHS lab... > > What is your daily flow for the work performed in your lab? > How are patients usually scheduled? (Are they staggered? All come in > at approximately the same time so 1st layers can be taken right away?) > How often are you receiving patient specimens? > Usually, how many patients per day? > Do you gross the tissue before freezing? > What is the goal time for slide completion? (From the time the tissue > is placed in the cryostat to freeze to the slide being ready to stain.) > How many levels are you placing on the slide? > How many slides per block are you cutting? > Do you have a set time limit on when all first layers are to be done? > > Any additional information is greatly appreciated. > > Thanks much!! > > Karen > > Karen L. Bauer HTL/HT (ASCP) > Histology Supervisor > Pathology Department > Mayo Clinic Health System in Eau Claire > E-mail: bauer.karen@mayo.edu > ___________________________________________ > Mayo Clinic Health System > 1221 Whipple St. > Eau Claire, WI 54703 > www.mayoclinichealthsystem.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shu-cheng.chen <@t> merck.com Tue Jul 26 12:56:20 2011 From: shu-cheng.chen <@t> merck.com (Chen, Shu-Cheng) Date: Tue Jul 26 12:56:24 2011 Subject: [Histonet] CRO for mouse brain paraffin sections Message-ID: <5D62649615FAA6478F801A08D10E51855C555F63BB@USCTMXP51003.merck.com> Hi, We are looking for a CRO experienced in step/serial sections of mouse brain and with a short turnaround time. Any recommendation is appreciated. Please contact me directly at shu-cheng.chen@merck.com Thanks, Shu-Cheng Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Bauer.Karen <@t> mayo.edu Tue Jul 26 13:13:39 2011 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Jul 26 13:14:04 2011 Subject: [Histonet] MOHS daily flow... In-Reply-To: References: <53FC421CC200C5429929EDE6C3676F300180813B@msgebe34> <1411.208.62.167.196.1311702003.squirrel@webmail.realpages.com> Message-ID: <53FC421CC200C5429929EDE6C3676F300180815F@msgebe34> Hi Jeanine, Our patients go back to a MOHS only waiting room between layers. Nice comfy loungers, big screen TV, refreshments, things to make them comfortable while they wait, since it could be a long day for them. They can bring in one family member with them if they want. Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.karen@mayo.edu ___________________________________________ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org -----Original Message----- From: Bartlett, Jeanine (CDC/OID/NCEZID) [mailto:jqb7@cdc.gov] Sent: Tuesday, July 26, 2011 12:53 PM To: Nicole Tatum; Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MOHS daily flow... That is very interesting. A friend had a procedure done last week and they kept him on the table until they were done. he did not go back to a waiting area. Is this common? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, July 26, 2011 1:40 PM To: Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] MOHS daily flow... Importance: Low Karen The number of patients depends on the surgeon. Some do 4-15 per day. It also depends on how many rooms you have to see patients. For the most part, Lets say you had two rooms available. and had 6 patients scheduled. schedule pt 15-20 mimutes apart. Bring first two in at same time. Doctor can get layers bandage pateints and place them in a waiting area. Next two patients come in get prepared and layers are taken. Place in waiting area. Next patients are brought in. layers taken. Place in waiting area. Each specmen after layer is taken is brought in lab. Mohs tech should be able to complete each case within 15-20minutes. Slides are placed for doc to read. Once he has taken all layers he can begin to read ready cases and take layers or begin closures. Pateints layers are taken and then they are placed back in waiting areas. This will continue until are patients are done. Tissue is grossed before it is placed in cryo. Two slides should be cut per peice of tissue. Not per case. We place 3 sections on each slide. Each section is a little deeper then the one before. We start at the frosted end. A great fast effecient tech should be able to handle 10-15 cases per day. I hope this helps. Nicole Tatum HT ASCP > > This is for those techs that assist in the MOHS lab... > > What is your daily flow for the work performed in your lab? > How are patients usually scheduled? (Are they staggered? All come in > at approximately the same time so 1st layers can be taken right away?) > How often are you receiving patient specimens? > Usually, how many patients per day? > Do you gross the tissue before freezing? > What is the goal time for slide completion? (From the time the tissue > is placed in the cryostat to freeze to the slide being ready to stain.) > How many levels are you placing on the slide? > How many slides per block are you cutting? > Do you have a set time limit on when all first layers are to be done? > > Any additional information is greatly appreciated. > > Thanks much!! > > Karen > > Karen L. Bauer HTL/HT (ASCP) > Histology Supervisor > Pathology Department > Mayo Clinic Health System in Eau Claire > E-mail: bauer.karen@mayo.edu > ___________________________________________ > Mayo Clinic Health System > 1221 Whipple St. > Eau Claire, WI 54703 > www.mayoclinichealthsystem.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Tue Jul 26 13:25:50 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Tue Jul 26 13:25:55 2011 Subject: [Histonet] Formalin down the drain?? In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DF04F1C5@LRGHEXVS1.practice.lrgh.org> References: <4940DF6D1C5FDF48931B6966AAEF939513083B@chimsx08.CHI.catholichealth.net><1311627234.95809.YahooMailClassic@web65704.mail.ac4.yahoo.com> <38667E7FB77ECD4E91BFAEB8D986386323DF04F1C5@LRGHEXVS1.practice.lrgh.org> Message-ID: Been reading all these responses...so funny how different places do different things. An old job I worked at poured gallons and gallons down the drain on a daily basis. I do think there was some kind of filter system in place, but all the same...how do you filter out all the little things?? I would say just follow whatever your EHS people say =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, July 26, 2011 10:15 AM To: Rene J Buesa; mtitford@aol.com; histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: RE: [Histonet] Formalin down the drain?? Not just OSHA, but the EPA prohibits it http://www.epa.gov/lawsregs/topics/waste.html#hazwaste Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 25, 2011 4:54 PM To: mtitford@aol.com; histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: RE: [Histonet] Formalin down the drain?? Your "rant" is interesting but wrong. OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous materials down the drain. I was also taken aback by Amy's posting. No, regardless of what your state law may or may not permit you to dump in the drain, you should not put some $avings over the well being of the environment and the drinking water of people. Formaldehyde is toxic and recently officially declared carcinogen. In the same way that "frackting" methods to obtain gas from shale has been deemed dangerous, equally dumping formaldehyde, xylene and any other chemical ought to be the source of concern. This in my "rant"! Ren? J. --- On Mon, 7/25/11, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: RE: [Histonet] Formalin down the drain?? To: mtitford@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 4:19 PM One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain..... you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to.... Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :)? You cannot "Like" this rant on Facebook or follow this rant on Twitter. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything ("and I mean everything") from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, "Hazardous materials in the histopathology laboratory" by Janet & Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tina.phan <@t> ventana.roche.com Tue Jul 26 15:39:09 2011 From: tina.phan <@t> ventana.roche.com (Phan, Tina) Date: Tue Jul 26 15:39:23 2011 Subject: [Histonet] Re: Histonet Digest, Vol 92, Issue 35 Message-ID: <704E6ADBC5DA1B458DE3EF44CF53D072010087739B@RNUMSEM721.nala.roche.com> My apologies to the members of Histonet! My blackberry had unfortunately dialed by accident. Tina (Phan) McLaughlin SYMPHONY Product Specialist Ventana Medical Systems, a Division of Roche Cell: 520.230.0175 www.ventanamed.com Sent by Verizon Blackberry ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Tue Jul 26 19:02:28 2011 Subject: Histonet Digest, Vol 92, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. LR White (Johnson, Teri) 2. Re: Where Can I Buy Pneumo Control Slides (Bob Richmond) 3. Re: Pneumocystis Control Slides (Oneil, Beth Ann) 4. Digital pathology systems (Breeden, Sara) ---------------------------------------------------------------------- Message: 1 Date: Tue, 26 Jul 2011 10:29:06 -0500 From: "Johnson, Teri" Subject: [Histonet] LR White To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9770566A9@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our H&E stains are a little bit pale. Thanks in advance! Teri ------------------------------ Message: 2 Date: Tue, 26 Jul 2011 11:30:59 -0400 From: Bob Richmond Subject: [Histonet] Re: Where Can I Buy Pneumo Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's "Pneumo"? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Tue, 26 Jul 2011 11:35:55 -0400 From: "Oneil, Beth Ann" Subject: [Histonet] Re: Pneumocystis Control Slides To: Message-ID: <052C41417E0EF64D8E201FE822D36CA90A1C5A0D@NT-EXCHANGE.wvuh.wvuhs.com> Content-Type: text/plain; charset="iso-8859-1" I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 4 Date: Tue, 26 Jul 2011 10:27:24 -0600 From: "Breeden, Sara" Subject: [Histonet] Digital pathology systems To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF91D@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was "attached" to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 35 **************************************** From sbreeden <@t> nmda.nmsu.edu Tue Jul 26 16:01:52 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jul 26 16:01:55 2011 Subject: [Histonet] Re: Histonet Digest, Vol 92, Issue 35 In-Reply-To: <704E6ADBC5DA1B458DE3EF44CF53D072010087739B@RNUMSEM721.nala.roche.com> References: <704E6ADBC5DA1B458DE3EF44CF53D072010087739B@RNUMSEM721.nala.roche.com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF922@nmdamailsvr.nmda.ad.nmsu.edu> I had this vision of your Blackberry in your back pocket --- well, you know... From algranth <@t> email.arizona.edu Tue Jul 26 16:09:19 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Jul 26 16:11:45 2011 Subject: [Histonet] Methasol Fast Blue Message-ID: We are looking for a vendor for Methasol Fast Blue. AKA Fast blue or Solvent Blue 24 and a protocol for a Modified Maxwell Stain. Some time ago - 2004 - there was a discussion on histonet about this stain. Margaret Blount had some protocols...Margaret if you are still on histonet can you email me and let me know about these protocols? I have some sort of protocol gleaned from a paper. I also can't locate any source that sells the stain. Thanks! Andi Grantham From k84as <@t> yahoo.com Tue Jul 26 16:17:45 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Tue Jul 26 16:17:49 2011 Subject: [Histonet] which conferences? Message-ID: <1311715065.68092.YahooMailClassic@web112612.mail.gq1.yahoo.com> dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and?a link if avaliable ? thanx From sdysart <@t> mirnarx.com Tue Jul 26 16:20:58 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Tue Jul 26 16:21:01 2011 Subject: [Histonet] which conferences? In-Reply-To: <1311715065.68092.YahooMailClassic@web112612.mail.gq1.yahoo.com> References: <1311715065.68092.YahooMailClassic@web112612.mail.gq1.yahoo.com> Message-ID: NSH!!! Great information...great people...great locations...and great nightly parties =) It's September 16 this year. http://s3.goeshow.com/nsh/annual/2011/index.cfm Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Tuesday, July 26, 2011 4:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] which conferences? dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and?a link if avaliable ? thanx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Tue Jul 26 16:22:35 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Jul 26 16:22:44 2011 Subject: [Histonet] which conferences? In-Reply-To: <1311715065.68092.YahooMailClassic@web112612.mail.gq1.yahoo.com> References: <1311715065.68092.YahooMailClassic@web112612.mail.gq1.yahoo.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F08FE8409CB@EXCHMB01.isis.local> NSH. Nsh.org Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Tuesday, July 26, 2011 2:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] which conferences? dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and?a link if avaliable ? thanx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Montina.VanMeter <@t> pbrc.edu Tue Jul 26 16:24:36 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Tue Jul 26 16:24:46 2011 Subject: [Histonet] which conferences? In-Reply-To: <1311715065.68092.YahooMailClassic@web112612.mail.gq1.yahoo.com> References: <1311715065.68092.YahooMailClassic@web112612.mail.gq1.yahoo.com> Message-ID: National Society for Histotechnology (Sept 16-21, 2011). www.nsh.org Montina J. Van Meter, HT (ASCP) Lab Manager Dept. of Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Tuesday, July 26, 2011 4:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] which conferences? dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and?a link if avaliable ? thanx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Tue Jul 26 16:30:08 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Jul 26 16:30:15 2011 Subject: [Histonet] which conferences? In-Reply-To: <1311715065.68092.YahooMailClassic@web112612.mail.gq1.yahoo.com> References: <1311715065.68092.YahooMailClassic@web112612.mail.gq1.yahoo.com> Message-ID: What type of histology interests you? Research or Clinical? Soft Tissue (paraffin) or Hard Tissue (resin)? I am giving a teleconference sponsored by the National Society for Histotechnolgy (NSH) next month (August 17th) as part of their VIR Summer Teleconference Series. During this teleconference I will be talking about the use of resin for undemineralized bone histology. Definitely check this out if you have interest in working with undemineralized bone! Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone Presented by Jack Ratliff, BioMimetic Therapeutics, Inc. As musculoskeletal research progresses with new technological advancements in the areas of biological repair and replacement, histological evaluation continues to play a crucial role in the determination of safety and efficacy for these new treatments. While most will employ traditional and acceptable methods of decalcification and paraffin embedding for the demonstration of these critical components of evaluation, these techniques can sometimes be very challenging and/or impossible when presented with a variety of implant materials or devices. For example, to evaluate safety and efficacy of a metallic device coated with a biological therapeutic at the bone interface, one will need to forego traditional methods of decalcification and seek an undisturbed representation of the specimen by utilizing an embedding media that is both as hard as the specimen and the implant material. Additionally, it may also be important to use a media that will not distort or dissolve the coating. This seminar will address the use of resin histology techniques for the demonstration of undemineralized bone. Topics will include tissue preparation, fixation, processing, infiltration, and embedding/polymerization with acrylic resins. We will also discuss two types of microtomy as related to small and large undemineralized bone specimens and the presence or absence of implant materials. Jack > Date: Tue, 26 Jul 2011 14:17:45 -0700 > From: k84as@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] which conferences? > > dear all > what conference do you recommend in histology and histotechnology feild do you prefer? > please provide me with date and a link if avaliable > > thanx > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfitz <@t> 007group.com Tue Jul 26 21:02:02 2011 From: cfitz <@t> 007group.com (Cathy) Date: Tue Jul 26 21:01:24 2011 Subject: [Histonet] repetitive wrist injury Message-ID: <3E5D97F1AA074E75BC32CD39E0D29446@your8ba846406f> Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. Cathy Kelowna, B.C. From rjr6 <@t> psu.edu Wed Jul 27 07:15:54 2011 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Jul 27 07:16:11 2011 Subject: [Histonet] repetitive wrist injury In-Reply-To: <3E5D97F1AA074E75BC32CD39E0D29446@your8ba846406f> References: <3E5D97F1AA074E75BC32CD39E0D29446@your8ba846406f> Message-ID: I recently went to the doctor about pain in my thumb (joint closest to the wrist) and was told it is arthritis and for opening jars I should get one of those kitchen jar openers. Either the under the counter or a hand held one. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Sent: Tuesday, July 26, 2011 10:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] repetitive wrist injury Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. Cathy Kelowna, B.C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WIMERH <@t> si.edu Wed Jul 27 08:42:06 2011 From: WIMERH <@t> si.edu (Wimer, Helen) Date: Wed Jul 27 08:42:13 2011 Subject: [Histonet] Microtome Repair Message-ID: Can anyone recommend a company that does microtome repair in the Washington, DC/ Maryland area? Thanks! Helen F Wimer HT (ASCP) Smithsonian Institution Department of Vertebrate Zoology Washington, DC (301) 496-1391 Wimerh@si.edu From wbenton <@t> cua.md Wed Jul 27 08:49:21 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Jul 27 08:50:06 2011 Subject: [Histonet] RE: Microtome Repair In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD92052FDBCD9@CUAEXH1.GCU-MD.local> DolbeyJamison BelAir Instruments Tech One and of course the manufacturer of the unit. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wimer, Helen [WIMERH@si.edu] Sent: Wednesday, July 27, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Repair Can anyone recommend a company that does microtome repair in the Washington, DC/ Maryland area? Thanks! Helen F Wimer HT (ASCP) Smithsonian Institution Department of Vertebrate Zoology Washington, DC (301) 496-1391 Wimerh@si.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Lynn.Burton <@t> Illinois.gov Wed Jul 27 08:57:10 2011 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Jul 27 08:59:25 2011 Subject: [Histonet] RE: Microtome Repair In-Reply-To: References: Message-ID: <4A6E2CACA1E017408EBA1B9911952CC003E12B58C7@IL084EXMBX214.illinois.gov> Tech One Biomedical offers services in that area. 1-866-497-3033 techone bs@attbi.com. We have used them for years with great success. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wimer, Helen [WIMERH@si.edu] Sent: Wednesday, July 27, 2011 8:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Repair Can anyone recommend a company that does microtome repair in the Washington, DC/ Maryland area? Thanks! Helen F Wimer HT (ASCP) Smithsonian Institution Department of Vertebrate Zoology Washington, DC (301) 496-1391 Wimerh@si.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Wed Jul 27 10:05:12 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Jul 27 10:05:21 2011 Subject: [Histonet] Microtome Repair In-Reply-To: Message-ID: Tech One Biomedical 866-497-3033 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Wimer, Helen" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/27/2011 09:42 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Microtome Repair Can anyone recommend a company that does microtome repair in the Washington, DC/ Maryland area? Thanks! Helen F Wimer HT (ASCP) Smithsonian Institution Department of Vertebrate Zoology Washington, DC (301) 496-1391 Wimerh@si.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From tjasper <@t> copc.net Wed Jul 27 11:01:31 2011 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Jul 27 11:01:36 2011 Subject: [Histonet] Sox 10 Message-ID: <90354A475B420441B2A0396E5008D49695ED4B@copc-sbs.COPC.local> General question - Is anyone out there running Sox 10 on the Ventana Benchmark XT? Any dilutions or protocols would be appreciated. Thanks, Tom Jasper Central Oregon Regional Path From WIMERH <@t> si.edu Wed Jul 27 11:04:35 2011 From: WIMERH <@t> si.edu (Wimer, Helen) Date: Wed Jul 27 11:05:24 2011 Subject: [Histonet] Microtome Repair In-Reply-To: References: , Message-ID: Thanks to all that responded to my question! Helen F Wimer HT (ASCP) Smithsonian Institution Department of Vertebrate Zoology Washington, DC (301) 496-1391 Wimerh@si.edu ________________________________________ From: DKBoyd@chs.net [DKBoyd@chs.net] Sent: Wednesday, July 27, 2011 11:05 AM To: Wimer, Helen Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Repair Tech One Biomedical 866-497-3033 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Wimer, Helen" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/27/2011 09:42 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Microtome Repair Can anyone recommend a company that does microtome repair in the Washington, DC/ Maryland area? Thanks! Helen F Wimer HT (ASCP) Smithsonian Institution Department of Vertebrate Zoology Washington, DC (301) 496-1391 Wimerh@si.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From lori.garcia <@t> medtronic.com Wed Jul 27 11:55:26 2011 From: lori.garcia <@t> medtronic.com (Garcia, Lori, Sr. Scientist) Date: Wed Jul 27 11:56:20 2011 Subject: [Histonet] repetitive wrist injury In-Reply-To: References: <3E5D97F1AA074E75BC32CD39E0D29446@your8ba846406f> Message-ID: <5A8A2A45BE610D459A95CD6A9102102A012521F22A@STSM1BMSGM04.ent.core.medtronic.com> I bought a big countertop jar opener a few years back for the same reason, only we have very large jars that we can't even get our little female hands around. The jar opener was supposed to open all sizes, but it wasn't able to open either our large ones or our small ones. Big waste! I hope you find something that works. Maybe one of us should invent jars that are really airtight and still easy to open! Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Wednesday, July 27, 2011 5:16 AM To: Cathy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] repetitive wrist injury I recently went to the doctor about pain in my thumb (joint closest to the wrist) and was told it is arthritis and for opening jars I should get one of those kitchen jar openers. Either the under the counter or a hand held one. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Sent: Tuesday, July 26, 2011 10:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] repetitive wrist injury Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. Cathy Kelowna, B.C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From Lynn.Burton <@t> Illinois.gov Wed Jul 27 13:14:48 2011 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Jul 27 13:16:24 2011 Subject: [Histonet] repetitive wrist injury In-Reply-To: <5A8A2A45BE610D459A95CD6A9102102A012521F22A@STSM1BMSGM04.ent.core.medtronic.com> References: <3E5D97F1AA074E75BC32CD39E0D29446@your8ba846406f> , <5A8A2A45BE610D459A95CD6A9102102A012521F22A@STSM1BMSGM04.ent.core.medtronic.com> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC003E12B58CC@IL084EXMBX214.illinois.gov> That could also be tendonitis related to the "snuff" joint. It starts with a D. Dequarveins or something, someone will know. I got it when I was pregnant with my second child. There are exercises for strengthening that tendon. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Lori, Sr. Scientist [lori.garcia@medtronic.com] Sent: Wednesday, July 27, 2011 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] repetitive wrist injury I bought a big countertop jar opener a few years back for the same reason, only we have very large jars that we can't even get our little female hands around. The jar opener was supposed to open all sizes, but it wasn't able to open either our large ones or our small ones. Big waste! I hope you find something that works. Maybe one of us should invent jars that are really airtight and still easy to open! Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Wednesday, July 27, 2011 5:16 AM To: Cathy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] repetitive wrist injury I recently went to the doctor about pain in my thumb (joint closest to the wrist) and was told it is arthritis and for opening jars I should get one of those kitchen jar openers. Either the under the counter or a hand held one. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Sent: Tuesday, July 26, 2011 10:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] repetitive wrist injury Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. Cathy Kelowna, B.C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Jul 27 13:22:42 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Jul 27 13:19:02 2011 Subject: [Histonet] repetitive wrist injury In-Reply-To: <5A8A2A45BE610D459A95CD6A9102102A012521F22A@STSM1BMSGM04.ent.core.medtronic.com> References: <3E5D97F1AA074E75BC32CD39E0D29446@your8ba846406f> <5A8A2A45BE610D459A95CD6A9102102A012521F22A@STSM1BMSGM04.ent.core.medtronic.com> Message-ID: <4E305772.4030902@umdnj.edu> You can try something called a strap wrench. It is a flexible strap that wraps around things like oil filters, smoke detectors and jar lids with a handle on the other end. Available at hardware, Home Depot and maybe your physical plant dept. Geoff On 7/27/2011 12:55 PM, Garcia, Lori, Sr. Scientist wrote: > I bought a big countertop jar opener a few years back for the same reason, only we have very large jars that we can't even get our little female hands around. The jar opener was supposed to open all sizes, but it wasn't able to open either our large ones or our small ones. Big waste! I hope you find something that works. Maybe one of us should invent jars that are really airtight and still easy to open! > Lori > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner > Sent: Wednesday, July 27, 2011 5:16 AM > To: Cathy; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] repetitive wrist injury > > I recently went to the doctor about pain in my thumb (joint closest to the wrist) and was told it is arthritis and for opening jars I should get one of those kitchen jar openers. Either the under the counter or a hand held one. > Roberta > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy > Sent: Tuesday, July 26, 2011 10:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] repetitive wrist injury > > Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. > > > > Cathy > > Kelowna, B.C. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > [CONFIDENTIALITY AND PRIVACY NOTICE] > > Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. > > To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From sbreeden <@t> nmda.nmsu.edu Wed Jul 27 13:23:50 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jul 27 13:23:54 2011 Subject: [Histonet] repetitive wrist injury In-Reply-To: <4E305772.4030902@umdnj.edu> References: <3E5D97F1AA074E75BC32CD39E0D29446@your8ba846406f><5A8A2A45BE610D459A95CD6A9102102A012521F22A@STSM1BMSGM04.ent.core.medtronic.com> <4E305772.4030902@umdnj.edu> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF92C@nmdamailsvr.nmda.ad.nmsu.edu> I've just added DeQuervain's tendonitis to my repertoire! It joins bilateral carpal tunnel (repaired successfully) and trigger finger (two repaired, three developing). You'd THINK I could say it's time to quit but the boss has threatened to put sugar in my gas tank. Oh, bother... From CIngles <@t> uwhealth.org Wed Jul 27 14:16:51 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Jul 27 14:17:50 2011 Subject: [Histonet] Biocare decloaker References: <661949901A768E4F9CC16D8AF8F2838C03974C3C@is-e2k3.grhs.net><4F8CD583-C4F4-4D53-9B29-2135D7201B49@yahoo.com> <92AD9B20A6C38C4587A9FEBE3A30E164082122AE0A@CHEXCMS10.one.ads.che.org> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A736@UWHC-MAIL2.uwhis.hosp.wisc.edu> I swear I blow a gasket at least once a week! Oh wait that's me, not the decloker. :) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Mon 7/25/2011 3:42 PM To: William; Mike Pence Cc: ; Diana McCaig Subject: RE: [Histonet] Biocare decloaker I wonder if a new gasket would help me.. I keep blowing mine! :>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William Sent: Monday, July 25, 2011 16:40 To: Mike Pence Cc: ; Diana McCaig Subject: Re: [Histonet] Biocare decloaker As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, "Mike Pence" wrote: > There is a huge difference between 122 degrees for 30 seconds and 90 > degrees for 45 minutes. I would say you need to look at something here! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana > McCaig > Sent: Monday, July 25, 2011 2:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biocare decloaker > > > We are trying to validate a Biocare decloaker and have found when we > use > 122 degrees for 30 seconds we get great signal, but distorted > morphology. > > > > If we reduce to 90 degrees for 45 minutes, the signal is > significantly decreased but the morphology is good. > > > > What protocols are you using? > > > > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkmarshall <@t> anthc.org Wed Jul 27 14:23:01 2011 From: kkmarshall <@t> anthc.org (Marshall, Kimberly K) Date: Wed Jul 27 14:24:09 2011 Subject: [Histonet] Cell block prep Message-ID: Hello Histo folks Would you all mind sharing with me your procedures for making cell blocks? I would like to compare different procedures with what I am doing. Thanks so much Kimberly From lblazek <@t> digestivespecialists.com Wed Jul 27 14:29:32 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jul 27 14:29:36 2011 Subject: [Histonet] Biocare decloaker In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A736@UWHC-MAIL2.uwhis.hosp.wisc.edu> References: <661949901A768E4F9CC16D8AF8F2838C03974C3C@is-e2k3.grhs.net><4F8CD583-C4F4-4D53-9B29-2135D7201B49@yahoo.com> <92AD9B20A6C38C4587A9FEBE3A30E164082122AE0A@CHEXCMS10.one.ads.che.org> <064F1ACBAE8A78469AE2E41D533D87E505A736@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EC15A39AC@IBMB7Exchange.digestivespecialists.com> I just sizzle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, July 27, 2011 3:17 PM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Biocare decloaker I swear I blow a gasket at least once a week! Oh wait that's me, not the decloker. :) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Mon 7/25/2011 3:42 PM To: William; Mike Pence Cc: ; Diana McCaig Subject: RE: [Histonet] Biocare decloaker I wonder if a new gasket would help me.. I keep blowing mine! :>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William Sent: Monday, July 25, 2011 16:40 To: Mike Pence Cc: ; Diana McCaig Subject: Re: [Histonet] Biocare decloaker As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, "Mike Pence" wrote: > There is a huge difference between 122 degrees for 30 seconds and 90 > degrees for 45 minutes. I would say you need to look at something here! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana > McCaig > Sent: Monday, July 25, 2011 2:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biocare decloaker > > > We are trying to validate a Biocare decloaker and have found when we > use > 122 degrees for 30 seconds we get great signal, but distorted > morphology. > > > > If we reduce to 90 degrees for 45 minutes, the signal is > significantly decreased but the morphology is good. > > > > What protocols are you using? > > > > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Wed Jul 27 16:56:25 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Jul 27 16:56:30 2011 Subject: [Histonet] LR White In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF9770566A9@EXCHMB-02.stowers-institute.org> References: <2C40E43D1F7A56408C4463FD245DDDF9770566A9@EXCHMB-02.stowers-institute.org> Message-ID: Teri, You should try using Haupt's Adhesive (www.dornandhart.com) to secure your resin sections. I have never (knocking twice on wood...LOL) lost a section during the staining of thin resin sections when using this product. In fact, I have used this solution for over 14 years and I even subject my methacrylate based resin (Acrylosin SOFT Embedding Solution @ www.dornandhart.com) to deplastification with warmed xylenes prior to staining and still do not lose a section. Also, I have found that "wrinkle free" is proportional to the softness of the resin block and the section collection, transfer and mounting method. I have attached a few images of specimens embedded with Acrylosin and stained H&E, Goldner's Trichrome, and Von Kossa - MacNeal's Tetrachrome for your consideration. Feel free to contact me if you have any additional questions. On a related note, I am giving a teleconference sponsored by the National Society for Histotechnology (NSH) next month (August 17th) as part of their VIR Summer Teleconference Series. During this teleconference I will be talking about the use of resin for undemineralized bone histology. Definitely check this out if you have interest in working with undemineralized bone! Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone Presented by Jack Ratliff, BioMimetic Therapeutics, Inc. As musculoskeletal research progresses with new technological advancements in the areas of biological repair and replacement, histological evaluation continues to play a crucial role in the determination of safety and efficacy for these new treatments. While most will employ traditional and acceptable methods of decalcification and paraffin embedding for the demonstration of these critical components of evaluation, these techniques can sometimes be very challenging and/or impossible when presented with a variety of implant materials or devices. For example, to evaluate safety and efficacy of a metallic device coated with a biological therapeutic at the bone interface, one will need to forego traditional methods of decalcification and seek an undisturbed representation of the specimen by utilizing an embedding media that is both as hard as the specimen and the implant material. Additionally, it may also be important to use a media that will not distort or dissolve the coating. This seminar will address the use of resin histology techniques for the demonstration of undemineralized bone. Topics will include tissue preparation, fixation, processing, infiltration, and embedding/polymerization with acrylic resins. We will also discuss two types of microtomy as related to small and large undemineralized bone specimens and the presence or absence of implant materials. Best Regards, Jack Jack L Ratliff Senior Histologist BioMimetic Therapeutics, Inc. Chairman, Hard Tissue Committee National Society for Histotechnology > From: TJJ@stowers.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 26 Jul 2011 10:29:06 -0500 > Subject: [Histonet] LR White > > Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our H&E stains are a little bit pale. > > Thanks in advance! > Teri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Jul 27 18:03:11 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jul 27 18:04:07 2011 Subject: [Histonet] Sox 10 In-Reply-To: <90354A475B420441B2A0396E5008D49695ED4B@copc-sbs.COPC.local> References: <90354A475B420441B2A0396E5008D49695ED4B@copc-sbs.COPC.local> Message-ID: <79837518C97F405B88BC601983C6BCB9@JoePC> would that be the Red Sox or the White Sox. Sorry, couldn't resist. Had a very bad day Joe ----- Original Message ----- From: "Thomas Jasper" To: Sent: Wednesday, July 27, 2011 11:01 AM Subject: [Histonet] Sox 10 General question - Is anyone out there running Sox 10 on the Ventana Benchmark XT? Any dilutions or protocols would be appreciated. Thanks, Tom Jasper Central Oregon Regional Path _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Wed Jul 27 18:02:49 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Jul 27 18:04:44 2011 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 07/27/2011 and will not return until 07/28/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From tkngflght <@t> yahoo.com Wed Jul 27 19:33:23 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jul 27 19:34:08 2011 Subject: [Histonet] TEMPS needed! Three techs and one really good grosser! Message-ID: <1311813203.12338.YahooMailNeo@web39413.mail.mud.yahoo.com> Hi everyone- ? We need a couple of good temps!? Three openings for regular histology (different places, different shifts) and one really really good grosser (CMS/CLIA Qualified--not sure just ask). ? We take care of everything?to keep you safe and happy on the road.? If you're curious, just call.? If you know someone who's talked about it--have them call!? I won't push--I promise.??Once we agree to move forward everything is in writing so you can relax and enjoy your assignment.? ? ???? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From Gervaip <@t> aol.com Wed Jul 27 21:22:52 2011 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Wed Jul 27 21:23:03 2011 Subject: [Histonet] repetitive wrist injury Message-ID: <37c6c.3000591f.3b6221fc@aol.com> Sounds like you have been long overdue for a motorized microtome, cover slipper, immuno stainer and some good ergo forceps. It really makes a difference. Why keep abusing your body and becoming a cripple. Pearl from New Orleans In a message dated 7/27/2011 1:24:49 P.M. Central Daylight Time, sbreeden@nmda.nmsu.edu writes: I've just added DeQuervain's tendonitis to my repertoire! It joins bilateral carpal tunnel (repaired successfully) and trigger finger (two repaired, three developing). You'd THINK I could say it's time to quit but the boss has threatened to put sugar in my gas tank. Oh, bother... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gervaip <@t> aol.com Wed Jul 27 21:30:39 2011 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Wed Jul 27 21:30:51 2011 Subject: [Histonet] repetitive wrist injury Message-ID: <37ffb.719fb2.3b6223cf@aol.com> The best thing I found was the cheapest .... at Bed Bath and Beyond., and I have tried them all. They are like 6x6 little rubber mats. I do find that one does not do the trick, my hands are so bad. So I use three of them and it really helps. And at times, because the jar wants to slip, I use one of the mats to hold the bottom of the jar, while turning the top with the others. The rubber mats are like those sheets you buy to put under rugs so they do not slip. We cut up rectangle pieces to place under our foot pedals (motorized microtomes)at work so they will not slip away on the floor. In a message dated 7/27/2011 1:19:34 P.M. Central Daylight Time, mcauliff@umdnj.edu writes: You can try something called a strap wrench. It is a flexible strap that wraps around things like oil filters, smoke detectors and jar lids with a handle on the other end. Available at hardware, Home Depot and maybe your physical plant dept. Geoff On 7/27/2011 12:55 PM, Garcia, Lori, Sr. Scientist wrote: > I bought a big countertop jar opener a few years back for the same reason, only we have very large jars that we can't even get our little female hands around. The jar opener was supposed to open all sizes, but it wasn't able to open either our large ones or our small ones. Big waste! I hope you find something that works. Maybe one of us should invent jars that are really airtight and still easy to open! > Lori > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner > Sent: Wednesday, July 27, 2011 5:16 AM > To: Cathy; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] repetitive wrist injury > > I recently went to the doctor about pain in my thumb (joint closest to the wrist) and was told it is arthritis and for opening jars I should get one of those kitchen jar openers. Either the under the counter or a hand held one. > Roberta > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy > Sent: Tuesday, July 26, 2011 10:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] repetitive wrist injury > > Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. > > > > Cathy > > Kelowna, B.C. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > [CONFIDENTIALITY AND PRIVACY NOTICE] > > Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. > > To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gervaip <@t> aol.com Wed Jul 27 21:32:12 2011 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Wed Jul 27 21:32:21 2011 Subject: [Histonet] repetitive wrist injury Message-ID: <380a3.2da0b053.3b62242c@aol.com> Pregnancy, because of fluid retention, can bring on repetitive motion injuries. In a message dated 7/27/2011 1:17:12 P.M. Central Daylight Time, Lynn.Burton@Illinois.gov writes: That could also be tendonitis related to the "snuff" joint. It starts with a D. Dequarveins or something, someone will know. I got it when I was pregnant with my second child. There are exercises for strengthening that tendon. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Lori, Sr. Scientist [lori.garcia@medtronic.com] Sent: Wednesday, July 27, 2011 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] repetitive wrist injury I bought a big countertop jar opener a few years back for the same reason, only we have very large jars that we can't even get our little female hands around. The jar opener was supposed to open all sizes, but it wasn't able to open either our large ones or our small ones. Big waste! I hope you find something that works. Maybe one of us should invent jars that are really airtight and still easy to open! Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Wednesday, July 27, 2011 5:16 AM To: Cathy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] repetitive wrist injury I recently went to the doctor about pain in my thumb (joint closest to the wrist) and was told it is arthritis and for opening jars I should get one of those kitchen jar openers. Either the under the counter or a hand held one. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Sent: Tuesday, July 26, 2011 10:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] repetitive wrist injury Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. Cathy Kelowna, B.C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gervaip <@t> aol.com Wed Jul 27 21:50:55 2011 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Wed Jul 27 21:51:09 2011 Subject: [Histonet] repetitive wrist injury Message-ID: <38972.70956053.3b62288f@aol.com> If you are doing this all day, everyday, your injuries have no way to go, but get worse. Repetitive motion injuries is also know as over use injuries. You are using the same muscles over and over without a break. Most likely you are using a fair amount of force to loosen the caps. And you are having to tighten your fist more because the jars are small. The only way to get relief and to stop the damage you are doing (and it can get to a point of no return) is 1. take frequent breaks and stretch your hands and fingers to let your hands recup 2. do not do it all day 3. do not do it everyday 4. have some one help you open/close jars or 5. change your occupation. The injuries can become more then just an aggravation, you can permanently lose the full use of your hands. In a message dated 7/26/2011 9:02:15 P.M. Central Daylight Time, cfitz@007group.com writes: Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. Cathy Kelowna, B.C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amitapandey <@t> torrentpharma.com Wed Jul 27 23:11:24 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Wed Jul 27 23:12:24 2011 Subject: [Histonet] repetitive wrist injury In-Reply-To: <37c6c.3000591f.3b6221fc@aol.com> References: <37c6c.3000591f.3b6221fc@aol.com> Message-ID: Pearl can you suggest me good ergo forceps for embedding. Amita From: Gervaip@aol.com To: sbreeden@nmda.nmsu.edu, mcauliff@umdnj.edu, lori.garcia@medtronic.com, histonet@lists.utsouthwestern.edu Date: 28/07/11 07:56 AM Subject: Re: [Histonet] repetitive wrist injury Sent by: histonet-bounces@lists.utsouthwestern.edu Sounds like you have been long overdue for a motorized microtome, cover slipper, immuno stainer and some good ergo forceps. It really makes a difference. Why keep abusing your body and becoming a cripple. Pearl from New Orleans In a message dated 7/27/2011 1:24:49 P.M. Central Daylight Time, sbreeden@nmda.nmsu.edu writes: I've just added DeQuervain's tendonitis to my repertoire! It joins bilateral carpal tunnel (repaired successfully) and trigger finger (two repaired, three developing). You'd THINK I could say it's time to quit but the boss has threatened to put sugar in my gas tank. Oh, bother... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michelle.Perrins <@t> uct.ac.za Thu Jul 28 02:39:11 2011 From: Michelle.Perrins <@t> uct.ac.za (Michelle Perrins) Date: Thu Jul 28 03:31:49 2011 Subject: [Histonet] repetitive stress injury Message-ID: <4E312E3F02000070001B9DBE@gwiasmtp.uct.ac.za> I was the only technologist who worked in a laboratory, so I was doing everything! I developed repetitive stress injury on my left wrist which got so bad I had to go to the doctor (I had DeQuervains tendonitis). After a cortisone injection and about 3 months rest of the hand, it helped tremendously. I also had a removable plastic cast to restrict movement. Luckily the work was done by another lab and I now have extra staff. The pain only comes back if it gets very cold, otherwise my wrist is fine now. Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: Michelle.Perrins@uct.ac.za ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### From dr_sadushe <@t> yahoo.com Thu Jul 28 08:06:30 2011 From: dr_sadushe <@t> yahoo.com (Sadushe Loxha) Date: Thu Jul 28 08:06:33 2011 Subject: [Histonet] (no subject) Message-ID: <1311858390.56690.YahooMailMobile@web36104.mail.mud.yahoo.com> http://www.ryweckn.com/blog/wp-content/themes/sendinvitetoyourfriend2.htm From Lynn.Burton <@t> Illinois.gov Thu Jul 28 08:36:04 2011 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Jul 28 08:36:42 2011 Subject: [Histonet] repetitive wrist injury In-Reply-To: <37c6c.3000591f.3b6221fc@aol.com> References: <37c6c.3000591f.3b6221fc@aol.com> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC003E12B58D1@IL084EXMBX214.illinois.gov> Thanks for all the great info Pearl! I forget some of those easy things day to day. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gervaip@aol.com [Gervaip@aol.com] Sent: Wednesday, July 27, 2011 9:22 PM To: sbreeden@nmda.nmsu.edu; mcauliff@umdnj.edu; lori.garcia@medtronic.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] repetitive wrist injury Sounds like you have been long overdue for a motorized microtome, cover slipper, immuno stainer and some good ergo forceps. It really makes a difference. Why keep abusing your body and becoming a cripple. Pearl from New Orleans In a message dated 7/27/2011 1:24:49 P.M. Central Daylight Time, sbreeden@nmda.nmsu.edu writes: I've just added DeQuervain's tendonitis to my repertoire! It joins bilateral carpal tunnel (repaired successfully) and trigger finger (two repaired, three developing). You'd THINK I could say it's time to quit but the boss has threatened to put sugar in my gas tank. Oh, bother... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Thu Jul 28 09:03:29 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Jul 28 09:03:35 2011 Subject: [Histonet] LR White In-Reply-To: References: <2C40E43D1F7A56408C4463FD245DDDF9770566A9@EXCHMB-02.stowers-institute.org> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9770566C1@EXCHMB-02.stowers-institute.org> Thanks for your information Jack. I will look into the Acrylosin as an option for doing MMA samples. We are using the LR White as an alternative to GMA. We have had lots of trouble with getting it to polymerize evenly and consistently. So now, of course, we are having issues with knife lines and wrinkling with this resin. I would be interested in getting your images if you would please send them to me directly. I believe the list serve blocks them. Best wishes, Teri ________________________________ From: Jack Ratliff [mailto:ratliffjack@hotmail.com] Sent: Wednesday, July 27, 2011 4:56 PM To: Johnson, Teri; Histonet Subject: RE: [Histonet] LR White Teri, You should try using Haupt's Adhesive (www.dornandhart.com) to secure your resin sections. I have never (knocking twice on wood...LOL) lost a section during the staining of thin resin sections when using this product. In fact, I have used this solution for over 14 years and I even subject my methacrylate based resin (Acrylosin SOFT Embedding Solution @ www.dornandhart.com) to deplastification with warmed xylenes prior to staining and still do not lose a section. Also, I have found that "wrinkle free" is proportional to the softness of the resin block and the section collection, transfer and mounting method. I have attached a few images of specimens embedded with Acrylosin and stained H&E, Goldner's Trichrome, and Von Kossa - MacNeal's Tetrachrome for your consideration. Feel free to contact me if you have any additional questions. On a related note, I am giving a teleconference sponsored by the National Society for Histotechnology (NSH) next month (August 17th) as part of their VIR Summer Teleconference Series. During this teleconference I will be talking about the use of resin for undemineralized bone histology. Definitely check this out if you have interest in working with undemineralized bone! Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone Presented by Jack Ratliff, BioMimetic Therapeutics, Inc. As musculoskeletal research progresses with new technological advancements in the areas of biological repair and replacement, histological evaluation continues to play a crucial role in the determination of safety and efficacy for these new treatments. While most will employ traditional and acceptable methods of decalcification and paraffin embedding for the demonstration of these critical components of evaluation, these techniques can sometimes be very challenging and/or impossible when presented with a variety of implant materials or devices. For example, to evaluate safety and efficacy of a metallic device coated with a biological therapeutic at the bone interface, one will need to forego traditional methods of decalcification and seek an undisturbed representation of the specimen by utilizing an embedding media that is both as hard as the specimen and the implant material. Additionally, it may also be important to use a media that will not distort or dissolve the coating. This seminar will address the use of resin histology techniques for the demonstration of undemineralized bone. Topics will include tissue preparation, fixation, processing, infiltration, and embedding/polymerization with acrylic resins. We will also discuss two types of microtomy as related to small and large undemineralized bone specimens and the presence or absence of implant materials. Best Regards, Jack Jack L Ratliff Senior Histologist BioMimetic Therapeutics, Inc. Chairman, Hard Tissue Committee National Society for Histotechnology > From: TJJ@stowers.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 26 Jul 2011 10:29:06 -0500 > Subject: [Histonet] LR White > > Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our H&E stains are a little bit pale. > > Thanks in advance! > Teri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nelsonrnch <@t> verizon.net Thu Jul 28 09:06:40 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Thu Jul 28 09:06:45 2011 Subject: [Histonet] BILLING FOR PATH Message-ID: <1311862000.89237.YahooMailRC@web84302.mail.re1.yahoo.com> Can anyone help me answer some questions regarding CPT?billing for?pathology? I would be so ever greatful. If you can please email me and I can be more specific. ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net From jennifer.harvey <@t> Vanderbilt.Edu Thu Jul 28 10:28:29 2011 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Thu Jul 28 10:28:34 2011 Subject: [Histonet] What is 10% Buffered Formalin Acetate Message-ID: I had a investigator say that he is using 10% Buffered Formalin Acetate to fix his mouse brains. They order it from Fisher (127-09-3). It is buffered with Sodium Acetate instead. Has anyone ever used this and why would you want to? I have never heard of it before! It is a new one to me! Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 From cpyse <@t> x-celllab.com Thu Jul 28 11:23:27 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Thu Jul 28 11:23:52 2011 Subject: [Histonet] cam 5.2 Message-ID: <002201cc4d42$ae638600$0b2a9200$@com> Hello Histonetters What company is everyone buying their Cam 5.2 antibody from? Thanks for the information. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com From marktarango <@t> gmail.com Thu Jul 28 11:27:16 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jul 28 11:27:21 2011 Subject: [Histonet] cam 5.2 In-Reply-To: <002201cc4d42$ae638600$0b2a9200$@com> References: <002201cc4d42$ae638600$0b2a9200$@com> Message-ID: We get ours from Becton Dickinson, product #349205. Mark On Thu, Jul 28, 2011 at 9:23 AM, Cynthia Pyse wrote: > Hello Histonetters > > What company is everyone buying their Cam 5.2 antibody from? Thanks for the > information. > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory/Histology Supervisor > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LSebree <@t> uwhealth.org Thu Jul 28 11:51:01 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jul 28 11:51:05 2011 Subject: [Histonet] cam 5.2 In-Reply-To: <002201cc4d42$ae638600$0b2a9200$@com> References: <002201cc4d42$ae638600$0b2a9200$@com> Message-ID: Ventana Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, July 28, 2011 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cam 5.2 Hello Histonetters What company is everyone buying their Cam 5.2 antibody from? Thanks for the information. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Jul 28 11:56:31 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jul 28 11:52:53 2011 Subject: [Histonet] What is 10% Buffered Formalin Acetate In-Reply-To: References: Message-ID: <4E3194BF.4040406@umdnj.edu> More likely buffered with calcium acetate. The late Ralph Lillie advocated 2% calcium acetate as a good buffer for formalin mixtures. One gets buffering plus some calcium ions to help with the stabilization of lipids.Also easy, just one weighing. Anything Lillie recommends is on a very sound scientific footing. Geoff On 7/28/2011 11:28 AM, Harvey, Jennifer Lynn wrote: > I had a investigator say that he is using 10% Buffered Formalin Acetate to fix his mouse brains. They order it from Fisher (127-09-3). It is buffered with Sodium Acetate instead. Has anyone ever used this and why would you want to? I have never heard of it before! It is a new one to me! > > Jennifer Harvey, HT(ASCP) QIHC > Vanderbilt University Medical Center > Neuropathology Lab Supervisor > C-2309 Medical Center North > Nashville, TN 37232-2561 > Phone: 615-343-0083 > Fax: 615-343-7089 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From marktarango <@t> gmail.com Thu Jul 28 11:53:59 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jul 28 11:54:02 2011 Subject: [Histonet] cam 5.2 In-Reply-To: References: <002201cc4d42$ae638600$0b2a9200$@com> Message-ID: I should have mentioned...we use it at 1:20 dilution. Mark On Thu, Jul 28, 2011 at 9:27 AM, Mark Tarango wrote: > We get ours from Becton Dickinson, product #349205. > > Mark > > On Thu, Jul 28, 2011 at 9:23 AM, Cynthia Pyse wrote: > >> Hello Histonetters >> >> What company is everyone buying their Cam 5.2 antibody from? Thanks for >> the >> information. >> >> >> >> Cindy Pyse, CLT, HT (ASCP) >> >> Laboratory/Histology Supervisor >> >> X-Cell Laboratories >> >> e-mail cpyse@x-celllab.com >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From TJJ <@t> stowers.org Thu Jul 28 15:10:23 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Jul 28 15:10:30 2011 Subject: [Histonet] Re: What is 10% Buffered Formalin Acetate Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9770566C9@EXCHMB-02.stowers-institute.org> Jennifer, the only reason I am aware of using acetate over phosphate buffering would be to minimize the precipitation that happens when phosphate buffered formalin contacts alcohol concentrations greater than 70%. It is why some automated tissue processors have a warm water flush step. The two fixes to this issue I have heard are: 1 - use 70% alcohol or lower in the step following the last fixative or 2 - use sodium acetate buffered formalin instead That's my wild guess for the day. I bet if you ask the investigator, the answer is nowhere close to this. Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From lab.path <@t> yahoo.com Thu Jul 28 15:34:45 2011 From: lab.path <@t> yahoo.com (Lab Path) Date: Thu Jul 28 15:34:49 2011 Subject: [Histonet] part-time histotech in Milwaukee Message-ID: <1311885285.23941.YahooMailNeo@web121217.mail.ne1.yahoo.com> Part-time histotech needed in Milwaukee, WI. ?The position offers flexible hours, good working environment, and negotiable compensation, but no benefits. If interested, please respond to lab.path@yahoo.com. Please indicate the hours you might be available, your salary requirements, and your educational background, work history, and certification status.? From abeharry798 <@t> gmail.com Thu Jul 28 16:13:07 2011 From: abeharry798 <@t> gmail.com (Andrea) Date: Thu Jul 28 16:09:03 2011 Subject: [Histonet] cam 5.2 In-Reply-To: References: <002201cc4d42$ae638600$0b2a9200$@com> Message-ID: <470AC13A-8D2A-4762-B6BD-FD94F381C950@gmail.com> Becton Dickinson On 2011-07-28, at 12:53 PM, Mark Tarango wrote: > I should have mentioned...we use it at 1:20 dilution. > > Mark > > On Thu, Jul 28, 2011 at 9:27 AM, Mark Tarango wrote: > >> We get ours from Becton Dickinson, product #349205. >> >> Mark >> >> On Thu, Jul 28, 2011 at 9:23 AM, Cynthia Pyse wrote: >> >>> Hello Histonetters >>> >>> What company is everyone buying their Cam 5.2 antibody from? Thanks for >>> the >>> information. >>> >>> >>> >>> Cindy Pyse, CLT, HT (ASCP) >>> >>> Laboratory/Histology Supervisor >>> >>> X-Cell Laboratories >>> >>> e-mail cpyse@x-celllab.com >>> >>> >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From margaret <@t> imebinc.com Thu Jul 28 16:29:47 2011 From: margaret <@t> imebinc.com (Margaret O'Donnell) Date: Thu Jul 28 16:24:07 2011 Subject: [Histonet] Position available; histotech for sales position Message-ID: <006901cc4d6d$79b99040$6d2cb0c0$@com> Well established company is looking for a histotech who would like to expand into the field of Sales. Regional Sales Representative for pathology laboratory sales to develop, manage, and execute to close, sales opportunities that support the Company's direction and goals. Principal Responsibilities Sell new and refurbished pathology laboratory equipment and consumables to hospitals, private laboratories, physicians offices and universities Laboratories include histology, cytology and general pathology facilities including autopsy/morgue suites. Generate leads for new accounts Develop business with key or named accounts and maintain existing sales Achieve quota requirements through planning and developing client relationships Identify and develop new opportunities through active cold calling and networking Meet and exceed budgeted sales targets and revenue objectives Develop and perform presentations to decision makers Serve as primary presenter of product demonstrations onsite Generate and maintain a credible forecast for accounts and provide this information on a regular basis to senior management Required Job Qualifications Business development skills, business preparation, sales negotiation, and relationship management Medical or lab experience preferred Self-disciplined professional with ability to achieve sales targets Accomplished speaker with successful presentation skills Demonstrated success in closing sales transactions in the healthcare environment preferred The sales representative will develop, manage, and execute to close, sales opportunities that support the Company's direction and goals. This is a results oriented sales position and will be reviewed every 90 days Position requires travel plus heavy phone solicitation and follow up Location: Southern California, Nevada and Arizona Compensation: Base plus Commission. Sales Reps can earn $50,000-$150,000 Per Year Car allowance Medical after six months employment Well regarded company with established accounts Send resume to hrimeb@gmail.com From k84as <@t> yahoo.com Thu Jul 28 17:14:44 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Thu Jul 28 17:14:48 2011 Subject: [Histonet] (no subject) Message-ID: <1311891284.65239.YahooMailMobile@web112606.mail.gq1.yahoo.com> http://cheap-recliners.com/sendinvitetoyourfriend1.htm From Marcia_Gaiser <@t> ssmhc.com Fri Jul 29 08:01:22 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Fri Jul 29 08:01:29 2011 Subject: [Histonet] Full Time HT Position in Oklahoma City Message-ID: <728F817C02110E498D803A7C3B0C6248068D43B5D1@S009-APEXM06.ds.ad.ssmhc.com> POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) ? or ? other nationally recognized certifying agency acceptable to the Laboratory Director ? or ? experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.com>, Ad# 10762, or contact Anna King at (405) 272-6105 for more information. Thank you! Anna King HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ From michaels <@t> janelia.hhmi.org Fri Jul 29 08:11:58 2011 From: michaels <@t> janelia.hhmi.org (Michael, Susan) Date: Fri Jul 29 08:12:02 2011 Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections Message-ID: I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions? Susan From settembr <@t> umdnj.edu Fri Jul 29 08:42:52 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Fri Jul 29 08:43:02 2011 Subject: [Histonet] Chlamydia Positive Control sold anywhere? In-Reply-To: References: <002201cc4d42$ae638600$0b2a9200$@com> Message-ID: Histonet, Looking for commercially available Chlamydia + control to be used with formalin fixed paraffin embedded human tissue. Does anyone know where that can be found? Thank you, Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ From ttruscot <@t> vetmed.wsu.edu Fri Jul 29 09:21:44 2011 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Jul 29 09:22:22 2011 Subject: [Histonet] RE: Cresyl violet stain on 50 um mouse brain sections In-Reply-To: References: Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00270A5B1BD3@CVM76.vetmed.wsu.edu> Hi Susan, I would suggest cutting lots thinner sections. The plus slides seem to have trouble holding on to very thick sections. Another possibility is too much glacial acetic acid in your working solution of cresyl-too much acid can loosen the bonds on the plus slides. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael, Susan Sent: Friday, July 29, 2011 6:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions? Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amario3 <@t> uic.edu Fri Jul 29 09:32:21 2011 From: amario3 <@t> uic.edu (Andrea Marion) Date: Fri Jul 29 09:32:25 2011 Subject: [Histonet] Re: 10% Buffered Formalin Acetate & PBS precipitation artifacts Message-ID: <3b515830e60dd2d8d06e67c96d1e791d.squirrel@webmail.uic.edu> Hi all, This discussion of phosphate buffered formalin has raised some other questions for me. In paraffin embedding protocols for early mouse embryos, I have seen recommendations that dehydration begin with steps in 30% and 50% EtOH in 0.9% NaCl or PBS, then the 70% and higher dilutions are in dH2O. I was told (and assumed) the purpose was to preserve osmolarity of the tissue, and minimize cell shrinkage - that this was a 'gentler' way of dehydrating. However, now it seems to me that at residual PBS precipitating in the 70% and higher dilutions would damage the tissue? What about unbuffered NaCl only? What does PBS precipitation artifact look like? Has anyone else encountered this practice, or perhaps can shed some light on the issue? Andrea Marion Graduate Student University of Illinois at Chicago --- Jennifer, the only reason I am aware of using acetate over phosphate buffering would be to minimize the precipitation that happens when phosphate buffered formalin contacts alcohol concentrations greater than 70%. It is why some automated tissue processors have a warm water flush step. The two fixes to this issue I have heard are: 1 - use 70% alcohol or lower in the step following the last fixative or 2 - use sodium acetate buffered formalin instead That's my wild guess for the day. I bet if you ask the investigator, the answer is nowhere close to this. Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From Ronald.Houston <@t> nationwidechildrens.org Fri Jul 29 09:36:38 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jul 29 09:36:47 2011 Subject: [Histonet] RE: Cresyl violet stain on 50 um mouse brain sections In-Reply-To: <9EF5279EBDFE6E4FB6605E8F183A00270A5B1BD3@CVM76.vetmed.wsu.edu> References: <9EF5279EBDFE6E4FB6605E8F183A00270A5B1BD3@CVM76.vetmed.wsu.edu> Message-ID: Susan, If you are talking about frozen sections of fixed tissue, they do not adhere well to slides (even charged slides) no matter how thick or thin. I'm not sure why you are treating the sections with alcohol chloroform either. That is pretty harsh treatment and totally unnecessary for Nissl staining. Why not stain the sections free-floating and then mount them from xylene? Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Friday, July 29, 2011 10:22 AM To: Michael, Susan; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cresyl violet stain on 50 um mouse brain sections Hi Susan, I would suggest cutting lots thinner sections. The plus slides seem to have trouble holding on to very thick sections. Another possibility is too much glacial acetic acid in your working solution of cresyl-too much acid can loosen the bonds on the plus slides. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael, Susan Sent: Friday, July 29, 2011 6:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions? Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From k84as <@t> yahoo.com Fri Jul 29 09:37:57 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Jul 29 09:38:02 2011 Subject: [Histonet] (no subject) Message-ID: <1311950277.70065.YahooMailMobile@web112609.mail.gq1.yahoo.com> http://www.dannlewis.net/wordpress/wp-content/themes/sendinvitetoyourfriend2.htm From cpyse <@t> x-celllab.com Fri Jul 29 09:41:22 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Jul 29 09:41:49 2011 Subject: [Histonet] Cam 5.2 Message-ID: <001201cc4dfd$962a21f0$c27e65d0$@com> Thanks to everyone for the Cam5.2 info. A great weekend to all. Cindy Cindy Pyse, CLT, HT (ASCP) Laobratory/Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com From lentwistle <@t> ucsd.edu Fri Jul 29 10:13:25 2011 From: lentwistle <@t> ucsd.edu (Entwistle, Laura) Date: Fri Jul 29 10:13:54 2011 Subject: [Histonet] RE: Cresyl violet stain on 50 um mouse brain sections In-Reply-To: References: Message-ID: We have done the same, but also adding a 50 and 70 ETOH as well. Our slides are also gel coated. We also have them in a 1 to 1 alcohol/chloroform, but only have them in for 30 minutes. Laura -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael, Susan Sent: Friday, July 29, 2011 6:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions? Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Jul 29 10:37:38 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jul 29 10:37:50 2011 Subject: [Histonet] Immunotech Message-ID: <4E329B82.7400.0077.1@harthosp.org> Does anyone know if Immunotech (France) is still around and, if so, do they have a distributor here in the US? We obtained an antibody to CD1a (clone O10) from them years ago through a distributor in Maine whose telephone number is no longer active. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From FUNKM <@t> mercyhealth.com Fri Jul 29 10:48:37 2011 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Fri Jul 29 10:49:09 2011 Subject: [Histonet] Sunquest CoPath Message-ID: <4E329005.E948.00AC.1@mercyhealth.com> We are looking at the Vantage system and how it relates with Sunquest CoPath. Please share with me concerns or issues if you are using these systems. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 From Rcartun <@t> harthosp.org Fri Jul 29 11:21:36 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jul 29 11:21:44 2011 Subject: [Histonet] cam 5.2 In-Reply-To: <002201cc4d42$ae638600$0b2a9200$@com> References: <002201cc4d42$ae638600$0b2a9200$@com> Message-ID: <4E32A5CF.7400.0077.0@harthosp.org> I have used CK-CAM5.2 for years with excellent results. However, for the past several years I have been using Leica-Microsystems' low molecular weight CK antibody, clone "5D3", that labels cytokeratins 8 and 18. I highly recommend it. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Cynthia Pyse" 7/28/2011 12:23 PM >>> Hello Histonetters What company is everyone buying their Cam 5.2 antibody from? Thanks for the information. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Montina.VanMeter <@t> pbrc.edu Fri Jul 29 11:22:36 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri Jul 29 11:25:43 2011 Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections Message-ID: Susan, I have stained 60um fixed sections on plus slides without any problem. I free float the sections in a multi-well plate containing PBS or TBS. When mounting the sections onto the plus slide, I wipe away the excess fluid after EACH section, so that the previous section will not be exposed to the PBS again which results in unequal drying. I also place the slide in a slanted position on the slide tray and allow them to dry overnight. The next morning you could also dry them in a 37 degree oven for a few minutes to help in adherence. I have had others in the lab mount sections at the same time as I do and have often observed that their sections will flap on the slide during staining. Mine, on the other hand, don't come off of the slide. I can only surmise that this is due to many years of experience in mounting sections and the importance of "wicking" away the excess buffer. Good luck, Tina -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael, Susan Sent: Friday, July 29, 2011 8:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions? Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbritten <@t> aol.com Fri Jul 29 12:02:45 2011 From: tbritten <@t> aol.com (Thomas Britten) Date: Fri Jul 29 12:02:57 2011 Subject: [Histonet] Immunotech In-Reply-To: <4E329B82.7400.0077.1@harthosp.org> References: <4E329B82.7400.0077.1@harthosp.org> Message-ID: <174339FF-0AEE-411E-B156-5D8E73ACBC6C@aol.com> Hi richard. I think immunotech was acquired by the now Beckmann coulter group. Tom Sent from my iPad On Jul 29, 2011, at 11:37 AM, "Richard Cartun" wrote: > Does anyone know if Immunotech (France) is still around and, if so, do they have a distributor here in the US? We obtained an antibody to CD1a (clone O10) from them years ago through a distributor in Maine whose telephone number is no longer active. Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lelmgren <@t> sunriselab.com Fri Jul 29 12:36:46 2011 From: lelmgren <@t> sunriselab.com (Laurie Elmgren) Date: Fri Jul 29 12:37:12 2011 Subject: [Histonet] Slide/cassette printers Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E30529B497@MailPDC.sunriselab.com> Is anyone having problems with the Thermo Printmate/ Slidemate and associated Cognex scanners? If so, what? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 250 Miller Place Hicksville, NY 11801 (631)435-1515x1018 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." From Vickroy.Jim <@t> mhsil.com Fri Jul 29 12:42:29 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Jul 29 12:42:40 2011 Subject: [Histonet] CD117 - FDA APPROVED PROTOCOL Message-ID: <24A4826E8EF0964D86BC5317306F58A55DF71EA8B3@mmc-mail.ad.mhsil.com> We are finding that the FDA approved protocol from Ventana does not stain some definite gi stromal tumors that stain quite well with another vendor's clone and protocol. My pathologists are trying to decide what to do with this. I would appreciate in hearing what kind of experience other labs have had when validating the FDA approved protocol for CD117. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From marktarango <@t> gmail.com Fri Jul 29 12:49:26 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Jul 29 12:49:31 2011 Subject: [Histonet] CD117 - FDA APPROVED PROTOCOL In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55DF71EA8B3@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A55DF71EA8B3@mmc-mail.ad.mhsil.com> Message-ID: We're sticking to our IVD CD117/c-kit rabbit poly from Dako. Mark On Fri, Jul 29, 2011 at 10:42 AM, Vickroy, Jim wrote: > We are finding that the FDA approved protocol from Ventana does not stain > some definite gi stromal tumors that stain quite well with another vendor's > clone and protocol. My pathologists are trying to decide what to do with > this. I would appreciate in hearing what kind of experience other labs > have had when validating the FDA approved protocol for CD117. > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kmerriam2003 <@t> yahoo.com Fri Jul 29 13:00:00 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Jul 29 13:00:04 2011 Subject: [Histonet] RNAscope ISH technology Message-ID: <1311962400.75572.YahooMailNeo@web130123.mail.mud.yahoo.com> Happy Friday! ? Does anyone have experience using RNAscope ISH technology on FFPE tissues? http://www.acdbio.com/whats_rnascope.html ? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From kmerriam2003 <@t> yahoo.com Fri Jul 29 13:00:50 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Jul 29 13:00:58 2011 Subject: [Histonet] molecular path email listserv Message-ID: <1311962450.41401.YahooMailNeo@web130106.mail.mud.yahoo.com> Hi Everyone, ? Does anyone know of an email listserv similar to this one for molecular pathology? ? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From Bryan.Watson <@t> neiurology.com Fri Jul 29 13:19:07 2011 From: Bryan.Watson <@t> neiurology.com (Bryan Watson) Date: Fri Jul 29 13:19:13 2011 Subject: [Histonet] ThinPrep Message-ID: <1C03BD76DC9BD14EA266F05C65D173B4430F32FC18@NEIU-SBS.Niu.lan> Does anyone know if the ThinPrep system has a reagent rental program available, rather than just purchasing the instrument? Thanks From plucas <@t> biopath.org Fri Jul 29 14:54:40 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Jul 29 14:49:07 2011 Subject: [Histonet] service on the Sukura Prisma Message-ID: <2E62C5204BA14103BC9654C45351971E@biopath.local> Hello, Does anyone know of an alternate vendor besides Sakura who can do the PM's and any necessary adjustments/fixes that come up on the Prisma? Sakura bills an arm and a leg for someone to come out. $175 dollars just for travel expenses is gouging to me, so if we can find someone else who can do the work at a cheaper rate, that would be great. Thanks for any info, Paula Lucas Lab Manager BP Labs From cathy.crumpton <@t> tuality.org Fri Jul 29 15:14:54 2011 From: cathy.crumpton <@t> tuality.org (Cathy Crumpton) Date: Fri Jul 29 15:15:00 2011 Subject: [Histonet] Histology lab assistant in CA Message-ID: Hello everyone, I have a wonderful histo lab assistant who is moving from Oregon to the Lathrop, CA area. We absolutely hate to see her go, but must. If there is anyone with an assistant position open in that area would you please let me know so I can pass the info on to her. She would be a real asset to your team. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 From tkngflght <@t> yahoo.com Fri Jul 29 17:03:46 2011 From: tkngflght <@t> yahoo.com (tkngflght@yahoo.com) Date: Fri Jul 29 17:04:04 2011 Subject: [Histonet] Goeff's bottle top issue... Message-ID: <533258.55429.qm@smtp102-mob.biz.mail.ac4.yahoo.com> One of our aids was so sweet-she would loosen the lids as she accessioned -just enough it didn't take a vise-grip to open eash one. But that is just transferring the problem. I've used the tacky rubber grippers on some types of jars: put the jar on one on the counter for traction, put another on top. With a flat palm press down HARD & rotate the lid.  Doesn't work with tiny jars but does alleviate the 'gripping' motion that you're having issues doing over & over for most common sizes. You can also use shelf liner cut to size. It coms in diffeent colors from the dollar store if you can't find the rubber disks things (looks like a floppy CD) Please forgive typos-tiny phone keys! Cheryl Kerry Full Staff Inc. -- Sent from my Palm Pre From gu.lang <@t> gmx.at Sat Jul 30 02:39:56 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Jul 30 02:40:22 2011 Subject: AW: [Histonet] molecular path email listserv In-Reply-To: <1311962450.41401.YahooMailNeo@web130106.mail.mud.yahoo.com> References: <1311962450.41401.YahooMailNeo@web130106.mail.mud.yahoo.com> Message-ID: I'm also interested in such a list/forum. Gudrun -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kim Merriam Gesendet: Freitag, 29. Juli 2011 20:01 An: Histonet; ihcrg@googlegroups.com Betreff: [Histonet] molecular path email listserv Hi Everyone,   Does anyone know of an email listserv similar to this one for molecular pathology?   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lscott <@t> sfcn.org Sat Jul 30 14:15:25 2011 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Sat Jul 30 14:15:29 2011 Subject: [Histonet] Thanks for all of the Microtome feedback Message-ID: <86601859-94D3-4380-921A-53CEA39CB7CD@sfcn.org> Thanks for all of the Microtome feedback, it has been very helpful. It sounds like everyone that has the RM 2235 loves it, and are not having a lot of problems. Our Leica RM 2235 has problems with blade holder tension. We worked with Leica when it was just about 14 months old but they were no help. The problem is the front portion of the blade holder that clamps the blade in place is too thin or just not made of strong enough material. We don't have much tension on the blade while sectioning but the plate starts to curl or bend at the top where it meets the blade. Then the blade tension is inconsistent and the sectioning is inconsistent. We have been using a Leica 2030 blade holder for the last few years but its a little too short. Any suggestions on a blade holder ???? Has anyone had similar issues with their RM 2235 ???? Thanks ! Scott Hendricksen HT(ASCP) From trathborne <@t> somerset-healthcare.com Sat Jul 30 14:37:45 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sat Jul 30 14:38:02 2011 Subject: [Histonet] Thanks for all of the Microtome feedback In-Reply-To: <86601859-94D3-4380-921A-53CEA39CB7CD@sfcn.org> References: <86601859-94D3-4380-921A-53CEA39CB7CD@sfcn.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F4FCB4@SMCMAIL01.somerset-healthcare.com> It must be out of warranty now, but why don't you price a new blade holder? Or better yet, ask Leica if they would replace the original one for you? If you do go to them, maybe you could request a different tech to evaluate it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: Saturday, July 30, 2011 3:15 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Thanks for all of the Microtome feedback Thanks for all of the Microtome feedback, it has been very helpful. It sounds like everyone that has the RM 2235 loves it, and are not having a lot of problems. Our Leica RM 2235 has problems with blade holder tension. We worked with Leica when it was just about 14 months old but they were no help. The problem is the front portion of the blade holder that clamps the blade in place is too thin or just not made of strong enough material. We don't have much tension on the blade while sectioning but the plate starts to curl or bend at the top where it meets the blade. Then the blade tension is inconsistent and the sectioning is inconsistent. We have been using a Leica 2030 blade holder for the last few years but its a little too short. Any suggestions on a blade holder ???? Has anyone had similar issues with their RM 2235 ???? Thanks ! Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. 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