[Histonet] fixation question for IHC

[Sherwood, Margaret]

I routinely freeze Karnovsky's fixative (paraformaldehyde and glutaraldehyde
mix) in small aliquots.  When you thaw, just vigorously shake to make sure all
is in solution.  Have had no problems with fixation.

Peggy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Margaret Blount
Sent: Monday, January 31, 2011 4:01 AM
To: Jan Berry; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] fixation question for IHC

It is possible to freeze paraformaldehyde in aliquots, so you don't have
to keep on making it up repeatedly as we did in the old days. I have
also seen that you can buy Pure formaldehyde, but I don't remember who
the supplier was. It was in sealed ampoules and was 16% so you could
dilute it in your preferred buffer. I have to say that this was about 10
years ago!

Margaret

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan
Berry
Sent: 28 January 2011 18:15
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fixation question for IHC

Is there a difference between using paraformaldehyde and neutral
buffered formalin when choosing a fixative for IHC?  I would prefer to
use formalin because of easier preparation, but am willing to put in the
extra time to make fresh paraformaldehyde solution if there is a
compelling reason.

Jan Berry
University of Michigan
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.