From pruegg <@t> ihctech.net Sat Jan 1 16:37:32 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jan 1 16:38:13 2011 Subject: [Histonet] methylene blue stained tissue In-Reply-To: <8E26873FB7498E44997E28F5F5EB62F332B2145A7D@UM-EMAIL06.um.umsystem.edu> References: <8E26873FB7498E44997E28F5F5EB62F332B2145A7D@UM-EMAIL06.um.umsystem.edu> Message-ID: <47914CBC2E9B4DEB80235F70DF65B7DA@prueggihctechlt> Should not be a problem if you are using hrp/dab brown or ap/red detection. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Quinn, Tim L. Sent: Thursday, December 30, 2010 12:30 PM To: histonet@lists.utsouthwestern.edu Cc: Herndon, Betty L. Subject: [Histonet] methylene blue stained tissue Hello Histies, I received rodent dermis that has been stained with methylene blue. I would like to perform immunolabeling experiments on this tissue. Should I expect any interference from the methylene blue stain? Tim Quinn Office of Research Administration MEDLAB Senior Research Technician/Central Lab Manager University of Missouri at Kansas City School of Medicine Room M3-202 2411 Holmes Street Kansas City, Missouri 64108-2792 Phone- 816-235-1990 or 816-235-1904 or FAX- 816-235-6517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Mon Jan 3 08:44:57 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Mon Jan 3 08:44:46 2011 Subject: [Histonet] Great Histology Jobs in NY Message-ID: <87224262.1294065897699.JavaMail.cfservice@SL4APP4> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From brian <@t> prometheushealthcare.com Mon Jan 3 09:37:36 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Mon Jan 3 09:37:50 2011 Subject: [Histonet] Histotech Opening in Memphis, TN Message-ID: <008401cbab5c$2b9e0bb0$82da2310$@com> Histotech Opening with a practice dedicated solely to the practice of gastrointestinal and liver pathology Position is in Memphis, TN Day shift Monday thru Friday either 8am to 5pm or 7am to 4pm Sign on bonus being offered! Please contact me today for immediate consideration. Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From making <@t> ufl.edu Mon Jan 3 10:52:12 2011 From: making <@t> ufl.edu (MKing) Date: Mon Jan 3 10:50:48 2011 Subject: [Histonet] Re: Histonet Digest, Vol 86, Issue 2 In-Reply-To: <201101021802.p02I20F5032425@smtp.ufl.edu> References: <201101021802.p02I20F5032425@smtp.ufl.edu> Message-ID: <4D21FEBC.9050009@ufl.edu> It may however if you're doing immunofluorescence, as MB has been used to quench autofluorescence (Seeing the Wood through the Trees: A Review of Techniques for Distinguishing Green Fluorescent Protein from Endogenous Autofluorescence; Nicholas Billinton and Andrew W. Knight, Analytical Biochemistry 291, 175?197 (2001). It isn't likely to alter antigen-antibody binding if that's what you meant. Mike King UF Pharmacology & Therapeutics > > Message: 1 > Date: Sat, 1 Jan 2011 15:37:32 -0700 > From: "Patsy Ruegg" > Subject: RE: [Histonet] methylene blue stained tissue > To: "'Quinn, Tim L.'" , > > Cc: "'Herndon, Betty L.'" > Message-ID: <47914CBC2E9B4DEB80235F70DF65B7DA@prueggihctechlt> > Content-Type: text/plain; charset="us-ascii" > > Should not be a problem if you are using hrp/dab brown or ap/red detection. > > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Quinn, Tim > L. > Sent: Thursday, December 30, 2010 12:30 PM > To: histonet@lists.utsouthwestern.edu > Cc: Herndon, Betty L. > Subject: [Histonet] methylene blue stained tissue > > Hello Histies, > > I received rodent dermis that has been stained with methylene blue. > > I would like to perform immunolabeling experiments on this tissue. > > Should I expect any interference from the methylene blue stain? > > Tim Quinn > From Reuel.Cornelia <@t> tsrh.org Mon Jan 3 17:04:40 2011 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Mon Jan 3 17:05:01 2011 Subject: [Histonet] Cryojane expertise Message-ID: <4D2201A8.B80A.00C5.1@tsrh.org> I need help with cryojane techniques on bone tissue.I have a hard time picking up the bone trabeculae area and bone marrow but the cartilage and other soft tissue are so easy to pick up. Any advice on the technique? Thank you. Reuel From JMacDonald <@t> mtsac.edu Tue Jan 4 00:54:57 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jan 4 00:55:03 2011 Subject: [Histonet] California Society Meeting Message-ID: Hi All, We were looking for speakers for our annual CSH symposium to be held May 13-15 in Concord, CA. In particular we need a safety related workshop. If you are interested in presenting a workshop please let me know and I will send you an application. Thank you, Jennifer MacDonald jmacdonald@mtsac.edu 909.274.4884 From dellav <@t> musc.edu Tue Jan 4 07:59:42 2011 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Jan 4 07:59:49 2011 Subject: [Histonet] c-kit mutation for Melanoma In-Reply-To: <47914CBC2E9B4DEB80235F70DF65B7DA@prueggihctechlt> References: <8E26873FB7498E44997E28F5F5EB62F332B2145A7D@UM-EMAIL06.um.umsystem.edu> <47914CBC2E9B4DEB80235F70DF65B7DA@prueggihctechlt> Message-ID: I'm interested in learning what labs you are using for c-kit mutation for Melanoma. I'm told this testing is different for c-kit in other organ systems and that labs performing c-kit may not be equipped to work up the melanoma variant Thank you for any light you can shed on this. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 From Barbara.Verstraeten <@t> ugent.be Tue Jan 4 08:14:27 2011 From: Barbara.Verstraeten <@t> ugent.be (Barbara Verstraeten) Date: Tue Jan 4 08:14:33 2011 Subject: [Histonet] custom-made polyclonal antibody doesn't stain Message-ID: <000601cbac19$b2834ba0$1789e2e0$@Verstraeten@ugent.be> Dear all, I had two antibodies made (in rabbit) for the same peptide. It was the first time I did this : the firm designed the immunization peptide, injected and bleed the rabbits and purified the antibody. I have tested it on paraffin sections: all background, no staining where it should be. I tried different retriever buffers and detection with dab and fluorescence. I have tested it on western blot: a lot of background, dirty lanes. To get a clearer view I performed a co-IP. Still not really great. Can anybody help me on this issue? I just want it to work for staining. Thanks a lot in advance! Barbara Verstraeten, Drs. Evolutionary Developmental Biology Department of Biology Ghent University K.L. Ledeganckstraat 35 B-9000 Ghent Belgium tel: ++32/(0)9 264 52 31 fax: ++32/(0)9 264 53 44 http://www.evodevo.ugent.be From cbarone <@t> NEMOURS.ORG Tue Jan 4 08:18:46 2011 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Jan 4 08:18:50 2011 Subject: [Histonet] History of Histology Message-ID: Histonetters....I am looking for the person who wrote History of Histology. I believe the author's name was either Mitchell or Michell? I found it on the web once, but have lost my copy? Anyone out there remember this article? CB From alisha <@t> ka-recruiting.com Tue Jan 4 08:23:53 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Jan 4 08:23:50 2011 Subject: [Histonet] High Paying, Exciting Histology Jobs in NY Message-ID: <696633057.1294151033473.JavaMail.cfservice@SL4APP4> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From jqb7 <@t> cdc.gov Tue Jan 4 08:24:05 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Jan 4 08:24:19 2011 Subject: [Histonet] History of Histology In-Reply-To: References: Message-ID: Just this one but a different author..... http://articles.adsabs.harvard.edu//full/1977HisSc..15...77B/0000077.000 .html Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Tuesday, January 04, 2011 9:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] History of Histology Importance: High Histonetters....I am looking for the person who wrote History of Histology. I believe the author's name was either Mitchell or Michell? I found it on the web once, but have lost my copy? Anyone out there remember this article? CB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brinkerk <@t> musc.edu Tue Jan 4 08:36:33 2011 From: brinkerk <@t> musc.edu (Geils, Karen Brinker) Date: Tue Jan 4 08:36:20 2011 Subject: [Histonet] HTL Distance Education Program Message-ID: The Medical University of South Carolina now offers a distance education (DE) option for HTL training. We currently have two DE students enrolled. The format of the program is designed for current employees in a histotechnology laboratory. The focus of the education is on the theory of histotechnology in order to prepare students for the HTL BOR exam. Evidence of clinical competence must be submitted throughout the program. Two start dates are available for DE students, April and September. We are currently accepting applications for the April 5th start date. For more information about the program, please see our website. If you have any questions about the program you may contact me via email or phone. Karen Geils Karen Brinker Geils, MS, HT(ASCP), CT(ASCP) Director, Histotechnology Program Department of Pathology and Laboratory Medicine Medical University of South Carolina 843-792-4013 From mcauliff <@t> umdnj.edu Tue Jan 4 08:41:41 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jan 4 08:38:50 2011 Subject: [Histonet] History of Histology In-Reply-To: References: Message-ID: <4D2331A5.5040903@umdnj.edu> Brian Bracegirdle wrote a good book on the history of histotechnology. Geoff Barone, Carol wrote: > Histonetters....I am looking for the person who wrote History of > Histology. I believe the author's name was either Mitchell or Michell? > I found it on the web once, but have lost my copy? Anyone out there > remember this article? CB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From sgoebel <@t> mirnarx.com Tue Jan 4 08:45:33 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Jan 4 08:45:37 2011 Subject: [Histonet] custom-made polyclonal antibody doesn't stain In-Reply-To: <000601cbac19$b2834ba0$1789e2e0$@Verstraeten@ugent.be> References: <000601cbac19$b2834ba0$1789e2e0$@Verstraeten@ugent.be> Message-ID: I have done this (or tried to do this) as well and almost ended up bald from ripping out my hair!! My first suggestion would be to try to get down to a 1/25 or 1/50 dilution. If the background starts to get too high then try blocking the Fc region. You can buy commercially available Fc blockers. I also tried conjugating the antibody with HRP which seemed to help some as well. Good Luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Verstraeten Sent: Tuesday, January 04, 2011 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] custom-made polyclonal antibody doesn't stain Dear all, I had two antibodies made (in rabbit) for the same peptide. It was the first time I did this : the firm designed the immunization peptide, injected and bleed the rabbits and purified the antibody. I have tested it on paraffin sections: all background, no staining where it should be. I tried different retriever buffers and detection with dab and fluorescence. I have tested it on western blot: a lot of background, dirty lanes. To get a clearer view I performed a co-IP. Still not really great. Can anybody help me on this issue? I just want it to work for staining. Thanks a lot in advance! Barbara Verstraeten, Drs. Evolutionary Developmental Biology Department of Biology Ghent University K.L. Ledeganckstraat 35 B-9000 Ghent Belgium tel: ++32/(0)9 264 52 31 fax: ++32/(0)9 264 53 44 http://www.evodevo.ugent.be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Jan 4 08:48:36 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jan 4 08:48:41 2011 Subject: [Histonet] RE: c-kit mutation for Melanoma In-Reply-To: References: <8E26873FB7498E44997E28F5F5EB62F332B2145A7D@UM-EMAIL06.um.umsystem.edu> <47914CBC2E9B4DEB80235F70DF65B7DA@prueggihctechlt> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640814F91361@CHEXCMS10.one.ads.che.org> Dr. Corless and staff at Oregon Health University do this testing. We send ours there for testing. Info at this link.. http://www.ohsu.edu/pathology/TransResLabAbout.php Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, January 04, 2011 09:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] c-kit mutation for Melanoma I'm interested in learning what labs you are using for c-kit mutation for Melanoma. I'm told this testing is different for c-kit in other organ systems and that labs performing c-kit may not be equipped to work up the melanoma variant Thank you for any light you can shed on this. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From marktarango <@t> gmail.com Tue Jan 4 08:48:45 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jan 4 08:48:49 2011 Subject: [Histonet] custom-made polyclonal antibody doesn't stain In-Reply-To: <-4213726502116747105@unknownmsgid> References: <-4213726502116747105@unknownmsgid> Message-ID: Hi Barabara, I'd suggest buying an antibody purifcation kit and then try staining with the antibody. I've had good luck trying this in the past. Here is a link to a kit http://www.piercenet.com/browse.cfm?fldID=4C752E8A-D886-4E12-A2B7-66C00F8B9B2A Good luck! Mark On Tue, Jan 4, 2011 at 6:14 AM, Barbara Verstraeten < Barbara.Verstraeten@ugent.be> wrote: > Dear all, > > > > I had two antibodies made (in rabbit) for the same peptide. > > It was the first time I did this : the firm designed the immunization > peptide, injected and bleed the rabbits and purified the antibody. > > I have tested it on paraffin sections: all background, no staining where it > should be. I tried different retriever buffers and detection with dab and > fluorescence. > > I have tested it on western blot: a lot of background, dirty lanes. To get > a > clearer view I performed a co-IP. Still not really great. > > > > Can anybody help me on this issue? > > I just want it to work for staining. > > > > Thanks a lot in advance! > > > > Barbara Verstraeten, Drs. > > Evolutionary Developmental Biology > > Department of Biology > > Ghent University > > K.L. Ledeganckstraat 35 > > B-9000 Ghent > > Belgium > > tel: ++32/(0)9 264 52 31 > > fax: ++32/(0)9 264 53 44 > > http://www.evodevo.ugent.be > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From billodonnell <@t> catholichealth.net Tue Jan 4 08:50:24 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Jan 4 08:50:39 2011 Subject: [Histonet] Masson's Trichrome Message-ID: Greetings, We are wanting to switch to a Masson's Trichrome method in our lab. Is anyone aware of a good source for a commercially prepared staining kit? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 From mpence <@t> grhs.net Tue Jan 4 08:55:03 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jan 4 08:55:07 2011 Subject: [Histonet] Masson's Trichrome In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974AE4@is-e2k3.grhs.net> I use to use the kit from Poly Scientific #k037. Stopped when we automated. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, January 04, 2011 8:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Masson's Trichrome Greetings, We are wanting to switch to a Masson's Trichrome method in our lab. Is anyone aware of a good source for a commercially prepared staining kit? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbeil1 <@t> hotmail.com Tue Jan 4 09:08:36 2011 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Tue Jan 4 09:08:39 2011 Subject: [Histonet] Surgipath I.B.F Tissue Fixative Message-ID: To all histotechs in the Orlando, FL area, I have eight gallons of Surgipath I.B.F. tissue fixative that I need to get rid off or give to a laboratory that could use it. The expiration date is 03/24/11. If you are interested please contact me offline. Thank you1 Damaris E. Beil, HT,ASCPcm, HTL Associates in Dermatology Histology Laboratory (407) 846-7546 ext. 3307 From micropathlabs <@t> yahoo.com Tue Jan 4 09:01:51 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Jan 4 09:28:34 2011 Subject: [Histonet] VIP 6 Users Message-ID: <509121.67688.qm@web57807.mail.re3.yahoo.com> Hi all. For those of you using the VIP 6,?how and when do you change or rotate reagents??I'm particularly interested in those who count?cassettes as a means of initiating reagent changes. We have mostly small biopsy specimens but?do use sponges frequently. I'm looking into reducing our reagent costs but, of course, will not sacrifice processing. Thank you. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From bakevictoria <@t> gmail.com Tue Jan 4 09:34:42 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Jan 4 09:34:48 2011 Subject: [Histonet] History of Histology In-Reply-To: <4D2331A5.5040903@umdnj.edu> References: <4D2331A5.5040903@umdnj.edu> Message-ID: Thophil Mitchell Prudden "A Manuel of Normal Histology" published in 1881. Vikki On Tue, Jan 4, 2011 at 9:41 AM, Geoff McAuliffe wrote: > Brian Bracegirdle wrote a good book on the history of histotechnology. > > Geoff > > > Barone, Carol wrote: > >> Histonetters....I am looking for the person who wrote History of >> Histology. I believe the author's name was either Mitchell or Michell? >> I found it on the web once, but have lost my copy? Anyone out there >> remember this article? CB >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria <@t> gmail.com Tue Jan 4 09:35:41 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Jan 4 09:35:46 2011 Subject: [Histonet] History of Histology In-Reply-To: References: <4D2331A5.5040903@umdnj.edu> Message-ID: Sorry typo Theophil is the correct spelling....need more tea! On Tue, Jan 4, 2011 at 10:34 AM, Victoria Baker wrote: > Thophil Mitchell Prudden "A Manuel of Normal Histology" published in 1881. > > Vikki > > On Tue, Jan 4, 2011 at 9:41 AM, Geoff McAuliffe wrote: > >> Brian Bracegirdle wrote a good book on the history of histotechnology. >> >> Geoff >> >> >> Barone, Carol wrote: >> >>> Histonetters....I am looking for the person who wrote History of >>> Histology. I believe the author's name was either Mitchell or Michell? >>> I found it on the web once, but have lost my copy? Anyone out there >>> remember this article? CB >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> >> >> >> -- >> -- >> ********************************************** >> Geoff McAuliffe, Ph.D. >> Neuroscience and Cell Biology >> Robert Wood Johnson Medical School >> 675 Hoes Lane, Piscataway, NJ 08854 >> voice: (732)-235-4583 mcauliff@umdnj.edu >> ********************************************** >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From mwalker <@t> vdxpathology.com Tue Jan 4 10:27:05 2011 From: mwalker <@t> vdxpathology.com (Melanie Walker) Date: Tue Jan 4 10:27:14 2011 Subject: [Histonet] Strange CD3 staining on K9 / Fel Intestine Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8498F6D@VDXSERVER01.vdxpathology.local> Hello Histonet, One of the pathologists here recently pointed out some strange staining occurring on both K9 and Fel tissue using the CD3 stain. There appeared to be very strong staining of the intestinal epithelium, all along the villi. The staining was comparable in strength to that of the positive T cells, does not appear as simple "fringe effect". We are using lymph node for the K9 control and Spleen for the Fel - both controls stained appropriately/ normally. I recently switched to a new lot of antibody - could this have such a strange effect? We are using rabbit polyclonal CD3 from Biocare and it has been working great for the past year or so. Has anyone ever encountered epithelial staining with a CD3 marker? In addition, I there appeared to be some non-specific nuclear staining with the CD79 marker on these same cases... I don't think this relates but is also a new problem for us (though I hear has been an ongoing problem for some). Melanie Walker From godsgalnow <@t> aol.com Tue Jan 4 10:36:40 2011 From: godsgalnow <@t> aol.com (=?utf-8?B?Z29kc2dhbG5vd0Bhb2wuY29t?=) Date: Tue Jan 4 10:36:38 2011 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gSFRMIERpc3RhbmNlIEVkdWNhdGlvbiBQcm9ncmFt?= Message-ID: <201101041636.p04GaOtS018886@imr-da02.mx.aol.com> What are the prerequisites to get in and what kind of degree if any, is obtained upon completion? Sent from my Verizon Wireless Phone ----- Reply message ----- From: "Geils, Karen Brinker" Date: Tue, Jan 4, 2011 9:36 am Subject: [Histonet] HTL Distance Education Program To: "histonet@lists.utsouthwestern.edu" The Medical University of South Carolina now offers a distance education (DE) option for HTL training. We currently have two DE students enrolled. The format of the program is designed for current employees in a histotechnology laboratory. The focus of the education is on the theory of histotechnology in order to prepare students for the HTL BOR exam. Evidence of clinical competence must be submitted throughout the program. Two start dates are available for DE students, April and September. We are currently accepting applications for the April 5th start date. For more information about the program, please see our website. If you have any questions about the program you may contact me via email or phone. Karen Geils Karen Brinker Geils, MS, HT(ASCP), CT(ASCP) Director, Histotechnology Program Department of Pathology and Laboratory Medicine Medical University of South Carolina 843-792-4013 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Jan 4 11:07:23 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jan 4 11:07:28 2011 Subject: [Histonet] Happy New Year from Pam Barker and RELIA Solutions Message-ID: <411BB61B5B9645BFB313DC015041679F@ownerf1abaad51> Hi Histonetters!! I want to wish you a very Promising, Prosperous and Terrific New Year in 2011. I recognize that in this current economy every little bit helps I really appreciate the leads that I get from you the subscribers to my histology bulletin and would like to give back to you so I am starting a referral program. I am offering a 500 dollar referral bonus for leads from you. I will pay you 500 dollars for every candidate that you refer to me who I place (that includes you) and every job lead you give me that I fill. I wanted to let you know that RELIA has expanded into permanent placement of Medical Technologists so if you know of any med techs or med tech openings the referral bonus applies to them as well! ***Shoot me an e-mail at relia1@earthlink.net or give me a call toll free at (866)-607-3542. for more details I also have a couple of new opportunities that might interest you or someone you know. Here are my newest opportunities: Histology Supervisor - Atlanta Histology Supervisor/IHC Specialist ? WI Histology Tech ? NC Dermpath Histotech ? NC I also have exciting supervisor opportunities in NH, CA, LA and CO and great tech positions in CA, TX, TN, NY and FL. Happy New Year to you and yours. Thanks! Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From rennie1108 <@t> yahoo.com Tue Jan 4 11:19:13 2011 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Tue Jan 4 11:19:17 2011 Subject: [Histonet] Need help with an old autostainer Message-ID: <605707.97530.qm@web59616.mail.ac4.yahoo.com> Hello, I know this is probably a long shot, but I'm trying to set up a stainer and don't have a user manual. It was bought at auction by my company, so who knows how old it is! It is a Microm DS 50, made by Zeiss (now Thermo Richard Allen). I've been trying to get my hands on a manual (having trouble setting up a staining program), but I don't quite know if I'm going about it the right way...or if there is one I can actually get. Does anyone know anything about this type of stainer. Please help! Thanks, Adrienne Anderson Phylogeny Inc. Columbus, OH From gagnone <@t> KGH.KARI.NET Tue Jan 4 11:24:21 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Jan 4 11:24:30 2011 Subject: [Histonet] Manual Coverslipping Safety Issues Message-ID: Has anyone successfully lobbied their institution for an automated coverslipper for safety reasons? Still coverslipping manually-stained IHC, neuro autopsy and special stains, sometimes hundreds per day. There has to be a better way. Under budget constraints. That's why I'm wondering if anyone has used concerns about histology staff safety, specifically techs under direct exposure to toluene/xylene, to enable purchase of an automated/robot coverslipper. I'd be interested in anyone's experience with this approach, successfully or unsuccessfully. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From mward <@t> wfubmc.edu Tue Jan 4 11:33:43 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Jan 4 11:33:58 2011 Subject: [Histonet] RE: Manual Coverslipping Safety Issues In-Reply-To: References: Message-ID: Yes we actually used the exposure to xylene to justify the purchase of a coverslipper in our lab. Ventilation in our lab is very poor to non-existent and we were successful in our push for the coverslipper. I also had an employee who was having difficulties with the fumes, etc. It has made a world of difference. Good luck! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Tuesday, January 04, 2011 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual Coverslipping Safety Issues Has anyone successfully lobbied their institution for an automated coverslipper for safety reasons? Still coverslipping manually-stained IHC, neuro autopsy and special stains, sometimes hundreds per day. There has to be a better way. Under budget constraints. That's why I'm wondering if anyone has used concerns about histology staff safety, specifically techs under direct exposure to toluene/xylene, to enable purchase of an automated/robot coverslipper. I'd be interested in anyone's experience with this approach, successfully or unsuccessfully. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Jan 4 11:38:44 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jan 4 11:38:49 2011 Subject: [Histonet] Manual Coverslipping Safety Issues In-Reply-To: Message-ID: This was back in the late 80's early 90's but I put together a proposal for a coverslipper at the institution that I worked at. I combined, increase in workload statistics, the time it took for the techs to coverslip verses the time it took for an automated coverslipper, improved efficiency and overall quality, and then I also added exposure to xylene into the mix and it worked. I do not think that you could do it on safety alone - unless you have data that supports over exposure to xylene fumes. I think you will need to add how it would improve efficiency and overall quality too. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Tuesday, January 04, 2011 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual Coverslipping Safety Issues Has anyone successfully lobbied their institution for an automated coverslipper for safety reasons? Still coverslipping manually-stained IHC, neuro autopsy and special stains, sometimes hundreds per day. There has to be a better way. Under budget constraints. That's why I'm wondering if anyone has used concerns about histology staff safety, specifically techs under direct exposure to toluene/xylene, to enable purchase of an automated/robot coverslipper. I'd be interested in anyone's experience with this approach, successfully or unsuccessfully. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Tue Jan 4 11:43:23 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Jan 4 11:43:30 2011 Subject: [Histonet] Manual Coverslipping Safety Issues Message-ID: I should add to my earlier post this morning: -we do use an automated coverslipper for our routine HPS staining, which is several hundred slides daily as well -we do have a fumehood over the main manual coverslipping area -we have had airborne solvent levels measured, but my concern relates to direct manual exposure i.e. hands in toluene -just because we can manually coverslip quickly doesn't mean the prolonged exposure is safe Thanks for your answers, keep 'em coming Eric >Has anyone successfully lobbied their institution for an automated coverslipper for safety reasons? >Still coverslipping manually-stained IHC, neuro autopsy and special stains, sometimes hundreds per day. There has to be a better way. Under budget constraints. That's why I'm wondering if anyone has used concerns about histology staff safety, specifically techs under direct exposure to toluene/xylene, to enable purchase of an automated/robot coverslipper. >I'd be interested in anyone's experience with this approach, successfully or unsuccessfully. >Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From micropathlabs <@t> yahoo.com Tue Jan 4 11:38:54 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Jan 4 11:45:38 2011 Subject: [Histonet] Manual Coverslipping Safety Issues In-Reply-To: References: Message-ID: <132561.25482.qm@web57808.mail.re3.yahoo.com> Sorry, no help here. We have lab assistants that manually coverslip and excellent ventilation in our newer facility. It is the position of the director that a lab assistant is cheaper than an automated instrument!?Go figure! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? ________________________________ From: "Gagnon, Eric" To: histonet@lists.utsouthwestern.edu Sent: Tue, January 4, 2011 12:24:21 PM Subject: [Histonet] Manual Coverslipping Safety Issues Has anyone successfully lobbied their institution for an automated coverslipper for safety reasons? Still coverslipping manually-stained IHC, neuro autopsy and special stains, sometimes hundreds per day. There has to be a better way.? Under budget constraints. That's why I'm wondering if anyone has used concerns about histology staff safety, specifically techs under direct exposure to toluene/xylene, to enable purchase of an automated/robot coverslipper. I'd be interested in anyone's experience with this approach, successfully or unsuccessfully. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Jan 4 11:52:47 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 4 11:53:26 2011 Subject: [Histonet] Manual Coverslipping Safety Issues In-Reply-To: Message-ID: If you're worried about hands being exposed to toluene, the argument would be to use gloves. You may find your TAT to be longer, in which case you could cite productivity as a reason for automation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gagnon, Eric Sent: Tuesday, January 04, 2011 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Manual Coverslipping Safety Issues I should add to my earlier post this morning: -we do use an automated coverslipper for our routine HPS staining, which is several hundred slides daily as well -we do have a fumehood over the main manual coverslipping area -we have had airborne solvent levels measured, but my concern relates to direct manual exposure i.e. hands in toluene -just because we can manually coverslip quickly doesn't mean the prolonged exposure is safe Thanks for your answers, keep 'em coming Eric >Has anyone successfully lobbied their institution for an automated coverslipper for safety reasons? >Still coverslipping manually-stained IHC, neuro autopsy and special stains, sometimes hundreds per day. There has to be a better way. Under budget constraints. That's why I'm wondering if anyone has used concerns about histology staff safety, specifically techs under direct exposure to toluene/xylene, to enable purchase of an automated/robot coverslipper. >I'd be interested in anyone's experience with this approach, successfully or unsuccessfully. >Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Randi.Hayes <@t> horizonnb.ca Tue Jan 4 12:13:59 2011 From: Randi.Hayes <@t> horizonnb.ca (Hayes, Randi (HorizonNB)) Date: Tue Jan 4 12:14:05 2011 Subject: [Histonet] Manual Coverslipping Safety Issues Message-ID: We haven't manually coverslipped here in almost 5 years. The cost of the coverslipper as well as the consumables is still cheaper that a lab assistant (at a full pro-rated wage including pension & benefits). We've also been able to extend it's usefulness to other departments in our laboratory who have stopped manually coverslipping their slides and instead put them on our instrument. Haematology (bone marrow aspirates) and Microbiology (parasitology) have both benefited tremendously. Safety is a huge concern but I don't think you are going to win your battle using that angle. Like it's been said in a previous post, good ventilation can solve that (as well as xylene resistant gloves). My experience in my own facility has been to break it down on paper in dollars and cents (sense, pun intended!) and no one can argue with saving some money (and you get consistent quality!) Good Luck! Randi Hayes Histology Supervisor Horizon Health Network Moncton, NB Canada ------- Horizon Health Network Disclaimer -------

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From katherine <@t> biospective.com  Tue Jan  4 12:31:39 2011
From: katherine <@t> biospective.com (Katherine Leonard)
Date: Tue Jan  4 12:31:44 2011
Subject: [Histonet] Tissue Processor Reagents
Message-ID: 

Hello histonetters ~

Is there any consensus regarding how often to change processor reagents? I
am using the TBS ATP1 tissue processor and the tissue size ranges from
biopsy to 1.5cm^3.

-- 
Katherine Leonard
Research Associate
From TJJ <@t> stowers.org  Tue Jan  4 12:45:30 2011
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Tue Jan  4 12:45:37 2011
Subject: [Histonet] Re: custom-made polyclonal antibody doesn't stain
Message-ID: 

Barbara,

I see your antibody was purified by the manufacturer already so I don't think it would be useful to purify it again. It sounds as though it's not a good antibody yet. How did the company test it, by elisa? Since your western and IP shows no specific staining, I'm not all that encouraged this antibody will be useful for IHC studies.

Maybe someone with more experience with this than me will chime in here. We did some testing of custom antibodies for one of our PIs and never got anything useful for her experiments. Others here have had great luck with theirs.

~Teri

From brinkerk <@t> musc.edu  Tue Jan  4 13:05:12 2011
From: brinkerk <@t> musc.edu (Geils, Karen Brinker)
Date: Tue Jan  4 13:05:26 2011
Subject: [Histonet] Clarification of MUSC HTL Program
Message-ID: 

Just for further clarification, the MUSC HTL program culminates in a post-baccalaureate certificate. The prerequisites are as follows:


 1.  Applicants must have a bachelor of science degree in biology, chemistry, microbiology, or related science field from an accredited college or university which includes:
    *   At least 30 semester hours of biology and chemistry courses (including microbiology course and immunology content, anatomy and physiology),
    *   One semester of organic chemistry (or biochemistry), and
    *   Three semester hours of college algebra or higher-level math.
 2.  A grade point average of at least 2.75 (on a 4.0 scale) on all college work and an average of at least 2.75 on all science courses are required for consideration.


Karen Brinker Geils, MS, HT(ASCP), CT(ASCP)
Director, Histotechnology Program
Department of Pathology and Laboratory Medicine
Medical University of South Carolina
843-792-4013

From Kim.Shields <@t> viha.ca  Tue Jan  4 13:13:40 2011
From: Kim.Shields <@t> viha.ca (Shields, Kim)
Date: Tue Jan  4 13:13:49 2011
Subject: [Histonet] IgG4
Message-ID: 

Looking for IgG4 for IHC-paraffin application.  We were using it from Invitrogen, but apparently, it is no longer available.   Any help is much appreciated.

Thanks,
Kim Shields
IHC Supervisor
Royal Jubilee Hospital
Victoria, BC

From billodonnell <@t> catholichealth.net  Tue Jan  4 13:20:28 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Tue Jan  4 13:20:39 2011
Subject: [Histonet] Manual Coverslipping Safety Issues
In-Reply-To: 
References: 
	
Message-ID: 

Eric,

I agree with Liz. There are several environmental controls that could be
put in place to help with expose to xylene. Many of   these controls
would cost far less than a coverslipper - so mention the health, but put
your eggs in the productivity/cost-saving basket. - 
William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Tuesday, January 04, 2011 11:39 AM
To: Gagnon, Eric; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Manual Coverslipping Safety Issues

This was back in the late 80's early 90's but I put together a proposal
for a coverslipper at the institution that I worked at.  I combined,
increase in workload statistics, the time it took for the techs to
coverslip verses the time it took for an automated coverslipper,
improved efficiency and overall quality, and then I also added exposure
to xylene into the mix and it worked.

I do not think that you could do it on safety alone - unless you have
data that supports over exposure to xylene fumes.  I think you will need
to add how it would improve efficiency and overall quality too.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC
PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303)
682-9060 www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon,
Eric
Sent: Tuesday, January 04, 2011 10:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Manual Coverslipping Safety Issues

Has anyone successfully lobbied their institution for an automated
coverslipper for safety reasons?
 
Still coverslipping manually-stained IHC, neuro autopsy and special
stains, sometimes hundreds per day. There has to be a better way.  Under
budget constraints. That's why I'm wondering if anyone has used concerns
about histology staff safety, specifically techs under direct exposure
to toluene/xylene, to enable purchase of an automated/robot
coverslipper.
 
I'd be interested in anyone's experience with this approach,
successfully or unsuccessfully.
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From thisisann <@t> aol.com  Tue Jan  4 13:21:34 2011
From: thisisann <@t> aol.com (thisisann@aol.com)
Date: Tue Jan  4 13:21:47 2011
Subject: [Histonet] Supervisor Competency Forms
Message-ID: <8CD7A5BBC32AD0A-73C-2D01@webmail-d054.sysops.aol.com>

Does anyone have a Histology Supervisor competency checklist they can share with me so I do not have to re-invent the wheel!  Thank you so much, ann


From spath.judith <@t> marshfieldclinic.org  Tue Jan  4 13:33:07 2011
From: spath.judith <@t> marshfieldclinic.org (Spath, Judith)
Date: Tue Jan  4 13:33:20 2011
Subject: [Histonet] question
Message-ID: <201101041933.p04JX4Zu018354@spamfilt>

Our lab has had water quality issues since moving in to a new building 2 years ago.  Would like to hear from fellow Histonetters on what type of water they are using for DI water and what type of unit are they getting it from?  First we had issues with the carbons in the water and now it seems to be the ions are off....our water may be TOO pure.....any help would be appreciated.  thanks,

Judy Spath, HTL, ASCP
Histology Manager
Marshfield Labs
Marshfield, WI 54449
1-715-221-6168


______________________________________________________________________
The contents of this message may contain private, protected and/or privileged information.  If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.  Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.  Thank you for your cooperation.
From kdboydhisto <@t> yahoo.com  Tue Jan  4 13:41:25 2011
From: kdboydhisto <@t> yahoo.com (Kelly Boyd)
Date: Tue Jan  4 13:41:29 2011
Subject: [Histonet] inventory
Message-ID: <13534.31600.qm@web58608.mail.re3.yahoo.com>


Can the labs out there tell me?IF and how you keep inventory of your special stains and immuno antibodies/reagents?

?
?
?


      
From jtaylor <@t> meriter.com  Tue Jan  4 13:47:55 2011
From: jtaylor <@t> meriter.com (Taylor, Jean)
Date: Tue Jan  4 13:48:00 2011
Subject: [Histonet] Eosinophil peroxidase antibody
Message-ID: 

Hi Everyone,

Is anyone using this antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation.

Thank you,

Jean Taylor, HT(ASCP)QIHC
IHC Tech.
Meriter Health Services
Madison, WI

From rennie1108 <@t> yahoo.com  Tue Jan  4 13:52:38 2011
From: rennie1108 <@t> yahoo.com (Adrienne Anderson)
Date: Tue Jan  4 13:52:42 2011
Subject: Fw: [Histonet] Need help with an old autostainer
Message-ID: <724745.54681.qm@web59602.mail.ac4.yahoo.com>

Thanks for all the great responses. I have found a manual and lots of advice!

Thanks,
Adrienne


----- Forwarded Message ----
From: Adrienne Anderson 
To: histonet@lists.utsouthwestern.edu
Sent: Tue, January 4, 2011 12:19:13 PM
Subject: [Histonet] Need help with an old autostainer

Hello,

I know this is probably a long shot, but I'm trying to set up a stainer and 
don't have a user manual. It was bought at auction by my company, so who knows 
how old it is! It is a Microm DS 50, made by Zeiss (now Thermo Richard Allen). 
I've been trying to get my hands on a manual (having trouble setting up a 
staining program), but I don't quite know if I'm going about it the right 
way...or if there is one I can actually get.

Does anyone know anything about this type of stainer. Please help!

Thanks,
Adrienne Anderson
Phylogeny Inc.
Columbus, OH 


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From LINDA.MARGRAF <@t> childrens.com  Tue Jan  4 14:05:09 2011
From: LINDA.MARGRAF <@t> childrens.com (Linda Margraf)
Date: Tue Jan  4 14:05:29 2011
Subject: [Histonet] histology position in Milwaukee, WI
Message-ID: <683621D7852C2F488898D0AC7F164A98063AF3CB@CMCPBEXMAIL02.Childrens.med>

Here's a message I was asked to post. Please don't reply to me.
Thanks, Linda M (Histonet administrator)


Please post the following job announcement.  Thanks you very much!

Histology Position in Milwaukee, WI

Please visit the link to learn more about the position.

https://tbe.taleo.net/NA3/ats/careers/requisition.jsp?org=MCW&cws=1&rid=5648

Contact:

A. Craig Mackinnon, M.D., Ph.D.
Director, Clinical and Translational Research Core Lab
a.craig.mackinnon@gmail.com
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From pruegg <@t> ihctech.net Tue Jan 4 14:16:29 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jan 4 14:17:12 2011 Subject: [Histonet] RE: [IHCRG] Eosinophil peroxidase antibody In-Reply-To: References: Message-ID: <9F8FC85F17F64C2486B13470CC5DDF4E@Patsyoffice> Jean, Is it anything different than just myeloperoxidase which is basically DAB, if you do DAB without h202 blocking it will stain all the myeloid cells including eosinophils, maybe this ab is specific just to eo's??? Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Taylor, Jean Sent: Tuesday, January 04, 2011 12:48 PM To: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' Subject: [IHCRG] Eosinophil peroxidase antibody Hi Everyone, Is anyone using this antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation. Thank you, Jean Taylor, HT(ASCP)QIHC IHC Tech. Meriter Health Services Madison, WI -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From sgoebel <@t> mirnarx.com Tue Jan 4 15:37:08 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Jan 4 15:37:13 2011 Subject: [Histonet] Tissue Processor Reagents In-Reply-To: References: Message-ID: I think the standard rule of thumb is 700-800 blocks? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katherine Leonard Sent: Tuesday, January 04, 2011 12:32 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Reagents Hello histonetters ~ Is there any consensus regarding how often to change processor reagents? I am using the TBS ATP1 tissue processor and the tissue size ranges from biopsy to 1.5cm^3. -- Katherine Leonard Research Associate _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Tue Jan 4 15:46:57 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Jan 4 15:47:04 2011 Subject: [Histonet] Fisher Microprobe Message-ID: Now that Fisher has discontinued manufacturing/distribution of the Microprobe system, does anyone know who if anyone still offers this? Or does anyone have one laying about that they no longer use? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From JMacDonald <@t> mtsac.edu Tue Jan 4 15:50:56 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jan 4 15:51:05 2011 Subject: [Histonet] Clarification of MUSC HTL Program In-Reply-To: Message-ID: Karen, Did you contact the NSH to have them include your program on their website? Jennifer "Geils, Karen Brinker" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/04/2011 11:08 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Clarification of MUSC HTL Program Just for further clarification, the MUSC HTL program culminates in a post-baccalaureate certificate. The prerequisites are as follows: 1. Applicants must have a bachelor of science degree in biology, chemistry, microbiology, or related science field from an accredited college or university which includes: * At least 30 semester hours of biology and chemistry courses (including microbiology course and immunology content, anatomy and physiology), * One semester of organic chemistry (or biochemistry), and * Three semester hours of college algebra or higher-level math. 2. A grade point average of at least 2.75 (on a 4.0 scale) on all college work and an average of at least 2.75 on all science courses are required for consideration. Karen Brinker Geils, MS, HT(ASCP), CT(ASCP) Director, Histotechnology Program Department of Pathology and Laboratory Medicine Medical University of South Carolina 843-792-4013 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Tue Jan 4 16:32:06 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Jan 4 16:32:10 2011 Subject: [Histonet] IgG4 In-Reply-To: References: Message-ID: Cell Marque now has an IgG4 IVD clone. It is one of the only ones I have found. On Tue, Jan 4, 2011 at 11:13 AM, Shields, Kim wrote: > Looking for IgG4 for IHC-paraffin application. We were using it from > Invitrogen, but apparently, it is no longer available. Any help is much > appreciated. > > Thanks, > Kim Shields > IHC Supervisor > Royal Jubilee Hospital > Victoria, BC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From FUNKM <@t> mercyhealth.com Tue Jan 4 16:35:58 2011 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Tue Jan 4 16:36:31 2011 Subject: [Histonet] CoPath users Message-ID: <4D234C84.9B87.00AC.0@mercyhealth.com> CoPath users could you please email your address to me. I would like to talk with labs that are using CoPath and chat with you about how you process and use some of the worklogs. We have had CoPath for many years but I have not had updates with labs to share some of how we have setup our process. Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 From alisha <@t> ka-recruiting.com Tue Jan 4 16:44:00 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Jan 4 16:44:02 2011 Subject: [Histonet] Histology Supervisor and Histotech Job in Maine Message-ID: <1964967437.1294181040154.JavaMail.cfservice@SL4APP1> Hi Histonet Members, I am a healthcare recruiter, specifically focused on the laboratory/pathology space. I have openings all across the country and would be interested in speaking with anyone interested in searching for a new job. Below is a great new opportunity that I am working on: Histotech and Histology Supervisor Opening in Maine: I am currently working on an amazing opportunity with one of largest most sophisticated labs in the Northeast. It is located in coastal Maine. This company is looking to hire on a Histology Supervisor and a Histotech. They currently are looking for someone with a Bachelor's Degree, histology experience, HTL(ASCP) or HT(ASCP) certified, and supervisory experience for the supervisor position. The compensation package is fantastic and includes health, dental, a retirement plan, and relocation assistance, if needed. If interested in learning more details, please email me at alisha@ka-recruiting.com. If you are not interested but know of someone you could recommend for this position, please pass on their contact information to me. We have a referral bonus for anyone you refer to me that I place into a new job. I am also working on the following histology/pathology jobs: * Technical Support Specialist - Western territory (need PA or HTL license) * Technical Support Specialist - PA/NY territory (need PA or HTL license) * Technical Support Specialist - Southeastern territory (need PA or HTL license) * MI - Dermpath Histology Manager * OK - Histology Supervisor * NV - Histotechnologist 3rd shift * NY - Long Island - Dermpath Lab Manager * NH - Histology Supervisor * Eastern OH - Histology Supervisor * Maine - Histotechnologist * Maine - Histology Supervisor * FL - Histotechnologist * NY City - Histology Supervisor with IHC experience * NY City - Histotechnologist * NY City - Grossing Tech * NV - Pathologist's Assistant If interested in any of the opportunities above, please email me. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From amosbrooks <@t> gmail.com Tue Jan 4 17:27:57 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Jan 4 17:28:07 2011 Subject: [Histonet] Manual Coverslipping Safety Issues In-Reply-To: <4d235ba1.0744960a.63a4.4213SMTPIN_ADDED@mx.google.com> References: <4d235ba1.0744960a.63a4.4213SMTPIN_ADDED@mx.google.com> Message-ID: <201101041827.57932.amosbrooks@gmail.com> Hi Eric, I used it as an additional reason in addition to cost control. The safety reasons are irrefutable, but you can't really put a price tag on it until you threaten to sue for a specific monitary amount or have OSHA threaten them with a specific fine. Business offices don't speak safety. You should add the safety concerns to an actual cost to savings ratio. If you time how long it takes you to coverslip a specific number of slides, you can determine how many slides you would cover in an hour. Taking the average salary per hour in your lab you would then determine how much you are spending in tech time in the lab per year. Comparing this to the cost of the equipment then you could determine how long it would take to recover the cost and have the machine pay for itself. Heartless pencil pushers just don't comprehend chemical hazards. It has to be a price game. Amos the jaded On Tuesday 04 January 2011 12:40:49 pm histonet- request@lists.utsouthwestern.edu wrote: > Message: 23 > Date: Tue, 4 Jan 2011 12:24:21 -0500 > From: "Gagnon, Eric" > Subject: [Histonet] Manual Coverslipping Safety Issues > To: > Message-ID: > ???????? > Content-Type: text/plain;???????charset="iso-8859-1" > > Has anyone successfully lobbied their institution for an automated > coverslipper for safety reasons? > Still coverslipping manually-stained IHC, neuro autopsy and special stains, > sometimes hundreds per day. There has to be a better way. ?Under budget > constraints. That's why I'm wondering if anyone has used concerns about > histology staff safety, specifically techs under direct exposure to > toluene/xylene, to enable purchase of an automated/robot coverslipper. > I'd be interested in anyone's experience with this approach, successfully > or unsuccessfully. > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada From marktarango <@t> gmail.com Tue Jan 4 18:06:56 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jan 4 18:07:02 2011 Subject: [Histonet] Pax-2 Message-ID: Hi Histonet, My lab needs to switch vendors on this antibody. Where is everyone buying Pax-2? I found on their website that Invitrogen sells an IVD rabbit poly Pax-2 antibody. Has anyone tried this or others? What about Epitomics RUO rabbit mono? Do all the antibodies give that cytoplasmic background staining that I've seen? I'd appreciate any info at all. Thanks Mark Tarango From gagnone <@t> KGH.KARI.NET Wed Jan 5 07:40:26 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Jan 5 07:40:36 2011 Subject: [Histonet] Manual Coverslipping Safety Issues Message-ID: A word of thanks for the plethora of responses regarding justification of additional automated coverslipping capacity for our laboratory. We will be considering all your suggestions, combining safety benefits with productivity gains to improve our workflow. You are all truly a valued and trustworthy resource. Thanks again, Eric Gagnon Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From Sharon.Davis-Devine <@t> carle.com Wed Jan 5 10:19:19 2011 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Jan 5 10:19:23 2011 Subject: [Histonet] Lab chairs Message-ID: We are getting new lab chairs for our Histology deparment and are wondering what other labs out there have used. We want something that has good ergonomic support, is not cloth, because of the paraffin and is not too expensive. Any suggestions or ideas are greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From liz <@t> premierlab.com Wed Jan 5 10:49:59 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 5 10:51:00 2011 Subject: [Histonet] Lab chairs In-Reply-To: Message-ID: Sharon We got chairs from VWR - catalog number 80086-440 VWR(r) Contour(tm) Self-Skinned Urethane Chairs, we got these back in 2007 or 2008 and they have held up great, they are comfortable and clean very easily. They might be a bit pricey. I think I paid around $150.00 to $200.00 for each back when I bought them and that was with special pricing from my rep. I see on line now that my pricing is $277.00 per chair and close to $400.00 if you do not have any discounted pricing with VWR. But overall they are great chairs. As histotechs we sit almost all day long depending upon what task we are performing so at the time I wanted to purchase good supportive chairs that I know we would be able to clean and keep for a while. So far it has been a good investment. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, January 05, 2011 9:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lab chairs We are getting new lab chairs for our Histology deparment and are wondering what other labs out there have used. We want something that has good ergonomic support, is not cloth, because of the paraffin and is not too expensive. Any suggestions or ideas are greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Cline <@t> wchsys.org Wed Jan 5 10:58:42 2011 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Wed Jan 5 11:04:45 2011 Subject: [Histonet] inventory In-Reply-To: <13534.31600.qm@web58608.mail.re3.yahoo.com> References: <13534.31600.qm@web58608.mail.re3.yahoo.com> Message-ID: We have two benches assigned to check and fill out inventory sheets once per week. One inventory deals with special stains and IHC along with other items that are in the same room. The other deals with accessioning, cutting and processing. Joyce Cline, H.T. (ASCP) Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cline@meritushealth.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhisto@yahoo.com] Sent: Tuesday, January 04, 2011 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inventory Can the labs out there tell me IF and how you keep inventory of your special stains and immuno antibodies/reagents? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From kdboydhisto <@t> yahoo.com Wed Jan 5 11:08:19 2011 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Jan 5 11:08:22 2011 Subject: [Histonet] inventory Message-ID: <284510.93623.qm@web58605.mail.re3.yahoo.com> Yesterday I inquired If and how labs did inventory for specials and immunos. I meant to ask IF and HOW labs do end of year inventory for specials and immunos. Thanks! ? ? ? ? From jaylundgren <@t> gmail.com Wed Jan 5 11:17:26 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Jan 5 11:17:56 2011 Subject: [Histonet] Lab chairs In-Reply-To: References: Message-ID: Good luck finding good task chairs that don't cost a fortune. The good ones are all very expensive now days. I think that buying cheap ones is a false savings, however, as they will fall apart in short order. I have personally seen a busy histo lab destroy new (cheap) chairs in a year. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Wed Jan 5 11:26:46 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Wed Jan 5 11:33:30 2011 Subject: [Histonet] Lab chairs In-Reply-To: References: Message-ID: <967402.42392.qm@web57806.mail.re3.yahoo.com> I have to?agree with Jay.?A couple of years ago, we purchased new, expensive lab chairs (from an office supply store that customized) and they look great even now. I think it's worth the?money from an ergonomic standpoint and also because they hold up much better. Just my 2 cents! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? ________________________________ From: Jay Lundgren To: Liz Chlipala Cc: histonet@lists.utsouthwestern.edu; Sharon.Davis-Devine Sent: Wed, January 5, 2011 12:17:26 PM Subject: Re: [Histonet] Lab chairs ? ? Good luck finding good task chairs that don't cost a fortune.? The good ones are all very expensive now days.? I think that buying cheap ones is a false savings, however, as they will fall apart in short order.? I have personally seen a busy histo lab destroy new (cheap) chairs in a year. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Sincerely, ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Jan 5 11:49:54 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jan 5 11:50:01 2011 Subject: [Histonet] inventory In-Reply-To: <284510.93623.qm@web58605.mail.re3.yahoo.com> References: <284510.93623.qm@web58605.mail.re3.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640814F91642@CHEXCMS10.one.ads.che.org> We do it annually. We use a combination of reports from our Materials Management Dept. and from our order spread sheet. (I keep a spread sheet of all items ordered). It's not totally accurate because of the chemicals and stains that have been here forever, but it's close enough for Mat Mgt. Best, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Wednesday, January 05, 2011 12:08 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inventory Yesterday I inquired If and how labs did inventory for specials and immunos. I meant to ask IF and HOW labs do end of year inventory for specials and immunos. Thanks! ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From pbaldwin <@t> micronenvironmental.com Wed Jan 5 12:21:58 2011 From: pbaldwin <@t> micronenvironmental.com (pbaldwin@MicronEnvironmental.com) Date: Wed Jan 5 12:22:08 2011 Subject: [Histonet] Manual Coverslipping Safety Issues Message-ID: <20110105102158.2F88F5B6@resin14.mta.everyone.net> Amos and Eric - you may this cost comparison chart in our December 20 blog ([1]http://greenchemistryforlife.wordpress.com) useful for attaching a dollar value to safety hazards in the lab. Not only is compliance with safe work practices necessary to protect employees (and others), but it is required by OSHA [2](http://www.osha.gov/SLTC/healthguidelines/xylene/recognition.html) . Peter Peter G. Baldwin Director of Operations, Marketing & Sales pbaldwin@MicronEnvironmental.com Micron Environmental Industries, Inc. Green Chemistry for Life www.MicronEnvironmental.com Blog - http://greenchemistryforlife.wordpress.com 1221 Cameron Street Alexandria, VA 22314 703-548-2776 703-548-7988/Fax References 1. http://greenchemistryforlife.wordpress.com/ 2. http://www.osha.gov/SLTC/healthguidelines/xylene/recognition.html From relia1 <@t> earthlink.net Wed Jan 5 12:45:37 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jan 5 12:45:41 2011 Subject: [Histonet] RELIA Hot Histology Job Alert - Exciting new Opptys in New York 01/05/2011 Message-ID: Hello Histonetters!!, I hope everybody is having a agreat day. I have some new opportunities to tell you about. I am currently working with a client on Long Island that is in need of several histo techs and cyto prep techs there are day and night shift positions available.. These are permanent full time positions in a busy growing lab. The client offers a great environment, a great manager and crew to work with and excellent compensation and benefits. My client requires at least 1 year of experience and a New York license. My question is do you know of anyone who might be interested in this position? If you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From thoward <@t> unm.edu Wed Jan 5 13:06:31 2011 From: thoward <@t> unm.edu (Tamara A Howard) Date: Wed Jan 5 13:06:36 2011 Subject: [Histonet] RE: Hazy background in IF Message-ID: I'm not certain that this will fix your problem, but I'd ditch the Tween in the final wash or two before mounting. There may be too much residual buffer on your slides when you add the Prolong - I wick off as much as I can, without drying the tissue, before mounting. You let the Prolong cure overnight - so why the nail polish? The solvents in nail polish can cause bleaching &/or "fuzziness" in the background & you don't need it if you are using a curing mount. If your slides aren't cured after an overnight "sit", you may just be using wayyy too much mounting media. As to why the problem has suddenly started cropping up....anybody's guess. Bad karma? Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From CIngles <@t> uwhealth.org Wed Jan 5 13:11:59 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Jan 5 13:12:02 2011 Subject: [Histonet] History of Histology References: <4D2331A5.5040903@umdnj.edu> Message-ID: This book, and a couple of others by Mitchell, are availible for free if you have a Nook (Barnes & Noble e-reader). You won't believe all the older (circa late 1800's, early 1900's) histo & pathology books. Nothing by Brian Bracegirdle though. There are plenty of interesting reads if time permits. Most of them cheap or free. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Victoria Baker Sent: Tue 1/4/2011 9:34 AM To: Geoff McAuliffe Cc: histonet@lists.utsouthwestern.edu; Barone, Carol Subject: Re: [Histonet] History of Histology Thophil Mitchell Prudden "A Manuel of Normal Histology" published in 1881. Vikki From sgoebel <@t> mirnarx.com Wed Jan 5 13:15:00 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Jan 5 13:15:04 2011 Subject: [Histonet] Stupid, stupid static!! Message-ID: So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From JMacDonald <@t> mtsac.edu Wed Jan 5 13:21:18 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 5 13:21:24 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: Message-ID: Static spray might work on the coats. To keep static at a minimum we keep wet paper towels in our waste tray on the microtome. The more moisture the better. Jennifer Sent by: histonet-bounces@lists.utsouthwestern.edu 01/05/2011 11:17 AM To cc Subject [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Jan 5 13:21:34 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jan 5 13:21:40 2011 Subject: [Histonet] RE: Stupid, stupid static!! In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640814F91684@CHEXCMS10.one.ads.che.org> Dryer sheets... And can you ground the microtome? Wire from metal to metal like a gas line or faucet, etc? Sorry for your frustration!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, January 05, 2011 14:15 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Lynn.Burton <@t> Illinois.gov Wed Jan 5 13:21:10 2011 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Jan 5 13:23:42 2011 Subject: [Histonet] RE: Stupid, stupid static!! In-Reply-To: References: Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00457078F@IL084EXMBX214.illinois.gov> Use fabric softener sheets. You can wipe the blades and knife holders with them. My daughter's American Girl magazine says to pour lotion in your hand, rub it between your hands and while it is still shiny run your hands through your hair to control static. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com [sgoebel@mirnarx.com] Sent: Wednesday, January 05, 2011 1:15 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Wed Jan 5 13:32:16 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jan 5 13:32:20 2011 Subject: [Histonet] RE: Stupid, stupid static!! References: <4A6E2CACA1E017408EBA1B9911952CC00457078F@IL084EXMBX214.illinois.gov> Message-ID: If you use lotion in the lab, won't it get on your slides and cause havoc (big oil droplets)? Jan Shivers ----- Original Message ----- From: "Burton, Lynn" To: ; Sent: Wednesday, January 05, 2011 1:21 PM Subject: [Histonet] RE: Stupid, stupid static!! Use fabric softener sheets. You can wipe the blades and knife holders with them. My daughter's American Girl magazine says to pour lotion in your hand, rub it between your hands and while it is still shiny run your hands through your hair to control static. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com [sgoebel@mirnarx.com] Sent: Wednesday, January 05, 2011 1:15 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HParker <@t> Skaggs.Net Wed Jan 5 13:32:37 2011 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Wed Jan 5 13:32:37 2011 Subject: [Histonet] Masson Trichrome In-Reply-To: <5d538058-2e52-4ec0-b3fe-5dfe8eda1712@email1.skaggs.net> References: <5d538058-2e52-4ec0-b3fe-5dfe8eda1712@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F506004700E68B9@email1.skaggs.net> We like the Sigma components. We buy them ala carte, though Poly Sci stuff is just the same. Helayne CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From Lynn.Burton <@t> Illinois.gov Wed Jan 5 13:37:36 2011 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Jan 5 13:38:22 2011 Subject: [Histonet] RE: Stupid, stupid static!! In-Reply-To: References: <4A6E2CACA1E017408EBA1B9911952CC00457078F@IL084EXMBX214.illinois.gov>, Message-ID: <4A6E2CACA1E017408EBA1B9911952CC004570791@IL084EXMBX214.illinois.gov> You will want to wash your hands after you do that but it really does cut the static. I also keep a spray bottle of DI water next to the microtome. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: Jan Shivers [shive003@umn.edu] Sent: Wednesday, January 05, 2011 1:32 PM To: Burton, Lynn; sgoebel@mirnarx.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Stupid, stupid static!! If you use lotion in the lab, won't it get on your slides and cause havoc (big oil droplets)? Jan Shivers ----- Original Message ----- From: "Burton, Lynn" To: ; Sent: Wednesday, January 05, 2011 1:21 PM Subject: [Histonet] RE: Stupid, stupid static!! Use fabric softener sheets. You can wipe the blades and knife holders with them. My daughter's American Girl magazine says to pour lotion in your hand, rub it between your hands and while it is still shiny run your hands through your hair to control static. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com [sgoebel@mirnarx.com] Sent: Wednesday, January 05, 2011 1:15 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Wed Jan 5 13:47:50 2011 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Jan 5 13:47:56 2011 Subject: [Histonet] Stupid, stupid static!! References: Message-ID: <90354A475B420441B2A0396E5008D49692BFD6@copc-sbs.COPC.local> Hi Sarah, One of our staff uses the static spray now and then. It works pretty well but the "fragrance" isn't the greatest. We also have a couple of humdifiers in the lab. These seem to help as our air in central Oregon is a bit dry on this side of the Cascades. Don't know if dry air is an issue for you in Austin, but we love our humidifiers here. Actually one is this cool blue penguin that shoots mist out of his beak...makes me smile every day. Later, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/617-2831 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, January 05, 2011 11:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Jan 5 14:00:25 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jan 5 14:01:15 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: <90354A475B420441B2A0396E5008D49692BFD6@copc-sbs.COPC.local> Message-ID: Where does one get a penguin? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Thomas Jasper Sent: Wednesday, January 05, 2011 2:48 PM To: sgoebel@mirnarx.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Stupid, stupid static!! Hi Sarah, One of our staff uses the static spray now and then. It works pretty well but the "fragrance" isn't the greatest. We also have a couple of humdifiers in the lab. These seem to help as our air in central Oregon is a bit dry on this side of the Cascades. Don't know if dry air is an issue for you in Austin, but we love our humidifiers here. Actually one is this cool blue penguin that shoots mist out of his beak...makes me smile every day. Later, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/617-2831 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, January 05, 2011 11:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From trathborne <@t> somerset-healthcare.com Wed Jan 5 11:11:09 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jan 5 14:01:35 2011 Subject: [Histonet] inventory In-Reply-To: <284510.93623.qm@web58605.mail.re3.yahoo.com> Message-ID: We have Cerner Millennium, and have a report built in it to calculate this information for any time period. It will give me the number of patient slides as well as control slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kelly Boyd Sent: Wednesday, January 05, 2011 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inventory Yesterday I inquired If and how labs did inventory for specials and immunos. I meant to ask IF and HOW labs do end of year inventory for specials and immunos. Thanks! ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From talulahgosh <@t> gmail.com Wed Jan 5 14:26:58 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jan 5 14:27:04 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: References: <90354A475B420441B2A0396E5008D49692BFD6@copc-sbs.COPC.local> Message-ID: Target! They have frogs too! I enjoy the elephant design the best. Well, next to Hello Kitty, but that's not for everyone. *http://tinyurl.com/2frkw3r* Emily Writer Richard Suflet recommended drinking large doses of strong vinegar with fleas to cure the illnesses that resulted from swallowing the horse-leeches that were common in drinking water. -Every Home a Distillery, Sarah Meacham On Wed, Jan 5, 2011 at 3:00 PM, Rathborne, Toni < trathborne@somerset-healthcare.com> wrote: > Where does one get a penguin? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> email.arizona.edu Wed Jan 5 14:46:04 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jan 5 14:46:16 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: References: <90354A475B420441B2A0396E5008D49692BFD6@copc-sbs.COPC.local> Message-ID: <275EC5C5-73D6-4938-B47D-E321A392C7A4@email.arizona.edu> I'm going to Target! We always have something going on here - static, heat and yes in certain months of the year we have humidity. Thanks for the humidifier idea. I'll spend the rest of the afternoon trying to decide if I want a frog or Hello Kitty. Cheers, Andi On Jan 5, 2011, at 1:26 PM, Emily Sours wrote: > Target! They have frogs too! I enjoy the elephant design the best. Well, > next to Hello Kitty, but that's not for everyone. > > *http://tinyurl.com/2frkw3r* > > Emily > > Writer Richard Suflet recommended drinking large doses of strong vinegar > with fleas to cure the illnesses that resulted from swallowing the > horse-leeches that were common in drinking water. > -Every Home a Distillery, Sarah Meacham > > > On Wed, Jan 5, 2011 at 3:00 PM, Rathborne, Toni < > trathborne@somerset-healthcare.com> wrote: > >> Where does one get a penguin? >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbreeden <@t> nmda.nmsu.edu Wed Jan 5 15:37:54 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jan 5 15:37:58 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: References: <90354A475B420441B2A0396E5008D49692BFD6@copc-sbs.COPC.local> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47558@nmdamailsvr.nmda.ad.nmsu.edu> This has SO many possible responses but I'll not go there. Someone might videotape it and leak it to the press... From espenceri <@t> yahoo.com Wed Jan 5 15:56:08 2011 From: espenceri <@t> yahoo.com (Beth Spenceri) Date: Wed Jan 5 15:56:22 2011 Subject: [Histonet] RE: Stupid, stupid static!! In-Reply-To: <4A6E2CACA1E017408EBA1B9911952CC00457078F@IL084EXMBX214.illinois.gov> Message-ID: <944048.50343.qm@smtp103-mob.biz.mail.ac4.yahoo.com> Agree with dryer sheets.  We keep one in each of our cryostats. -beth -- Sent from my Palm Pre On Jan 5, 2011 1:24 PM, Burton, Lynn <Lynn.Burton@Illinois.gov> wrote: Use fabric softener sheets. You can wipe the blades and knife holders with them. My daughter's American Girl magazine says to pour lotion in your hand, rub it between your hands and while it is still shiny run your hands through your hair to control static. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com [sgoebel@mirnarx.com] Sent: Wednesday, January 05, 2011 1:15 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gervaip <@t> aol.com Wed Jan 5 18:39:02 2011 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Wed Jan 5 18:39:16 2011 Subject: [Histonet] Stupid, stupid static!! Message-ID: <4f0ed.366457b5.3a566926@aol.com> wipe the microtome and knife holder with a Bounce laundry softener sheet. It works for us. In a message dated 1/5/2011 3:38:19 P.M. Central Standard Time, sbreeden@nmda.nmsu.edu writes: This has SO many possible responses but I'll not go there. Someone might videotape it and leak it to the press... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Cameron.Nowell <@t> ludwig.edu.au Wed Jan 5 19:06:02 2011 From: Cameron.Nowell <@t> ludwig.edu.au (Cameron Nowell) Date: Wed Jan 5 19:06:06 2011 Subject: [Histonet] Slide Labeling System that Survives Citrate Boiling Message-ID: Hi List, I am looking for a slide labelling system to print labels for our research histology samples that will survive pretty much anything we throw at it. There seems to be lots of choices out there for chemical resistant labels but i can't seem to find much on ones that are resistant to antigen retrieval like citrate boiling. I have searched google and the histonet archives and the best i can find is a reference to General Data having some that may do the job. Does anyone out there have any more info or are you using someting that works well? Thanks Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3155 Mobile: +61422882700 Fax: +61 3 9341 3104 Facility Website This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd. From talulahgosh <@t> gmail.com Wed Jan 5 20:24:17 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jan 5 20:24:23 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: <4f0ed.366457b5.3a566926@aol.com> References: <4f0ed.366457b5.3a566926@aol.com> Message-ID: You know, all of it sounds so easy...unless you're cryosectioning!! Does putting a humidifier outside help with static in a cryostat? Because the static is driving me nuts. Why aren't there teams of people working on this issue? It keeps coming up, yet there is no one good answer, Calling all engineers!! Emily Writer Richard Suflet recommended drinking large doses of strong vinegar with fleas to cure the illnesses that resulted from swallowing the horse-leeches that were common in drinking water. -Every Home a Distillery, Sarah Meacham On Wed, Jan 5, 2011 at 7:39 PM, wrote: > wipe the microtome and knife holder with a Bounce laundry softener sheet. > It works for us. > > > In a message dated 1/5/2011 3:38:19 P.M. Central Standard Time, > sbreeden@nmda.nmsu.edu writes: > > This has SO many possible responses but I'll not go there. Someone > might videotape it and leak it to the press... > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From louise.renton <@t> gmail.com Thu Jan 6 00:39:19 2011 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Jan 6 00:39:27 2011 Subject: [Histonet] Slide Labeling System that Survives Citrate Boiling In-Reply-To: References: Message-ID: long ago there was a cute system that worked with superfrost type slides - you know the ones that have the sort of coloured ends - etched through the paint to make a permanent marking Common HB pencil also survives on ordinary frosted slidesand surgipath pen marking esp if used on superfrost slides On 1/6/11, Cameron Nowell wrote: > Hi List, > > > > I am looking for a slide labelling system to print labels for our > research histology samples that will survive pretty much anything we > throw at it. There seems to be lots of choices out there for chemical > resistant labels but i can't seem to find much on ones that are > resistant to antigen retrieval like citrate boiling. I have searched > google and the histonet archives and the best i can find is a reference > to General Data having some that may do the job. Does anyone out there > have any more info or are you using someting that works well? > > > > Thanks > > > > Cam > > > > > > > > Cameron J. Nowell > Microscopy Manager > Centre for Advanced Microscopy > Ludwig Institute for Cancer Research > PO Box 2008 > Royal Melbourne Hospital > Victoria, 3050 > AUSTRALIA > > Office: +61 3 9341 3155 > Mobile: +61422882700 > Fax: +61 3 9341 3104 > > Facility Website > > > > > > > > This communication is intended only for the named recipient and may contain > information that is confidential, legally privileged or subject to > copyright; the Ludwig Institute for Cancer Research Ltd does not waive any > rights if you have received this communication in error. > The views expressed in this communication are those of the sender and do not > necessarily reflect the views of the Ludwig Institute for Cancer Research > Ltd. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From lloyd.3 <@t> osu.edu Thu Jan 6 05:34:58 2011 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Thu Jan 6 05:35:02 2011 Subject: [Histonet] Research charges Message-ID: <04A71EFE05FE5F4DAA05D0500FD0597002A016DF@Distal.dentnet.dent.ohio-state.edu> Would anyone like to share their charges for research. I am interested in these charges. Routine processing Hand processing H&E Unstained slides Routine Special Stains (PAS,GMS etc.) I would also like to know policies on charging for repeat work. If a researcher is interested in an area(bone) that is difficult and needs to be recut over and over again because of lifting and folding in the area of interest. After using many different techniques I have resolved the problem but some researchers demand more and more for free. Also I have a couple of researchers that stand with me while I am cutting through the block to keep the lesional slides only in their blocks. It takes around 45 minutes per block. Is their any compensation for the time I spend with the researcher. I put through some new policies and prices but my pathologist says I need documentation of those charges. Thanks Mary From LSebree <@t> uwhealth.org Thu Jan 6 07:32:02 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jan 6 07:32:07 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: <275EC5C5-73D6-4938-B47D-E321A392C7A4@email.arizona.edu> References: <90354A475B420441B2A0396E5008D49692BFD6@copc-sbs.COPC.local> <275EC5C5-73D6-4938-B47D-E321A392C7A4@email.arizona.edu> Message-ID: <8C023B4AB999614BA4791BAEB26E273839A16A@UWHC-MAIL01.uwhis.hosp.wisc.edu> Be careful about buying a humidifier. I brought a brand new one in (small table top type) that I had purchased but because it didn't have a grounded plug it wasn't allowed; never did find one with a grounded plug. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, January 05, 2011 2:46 PM To: undisclosed-recipients Cc: HISTONET Subject: Re: [Histonet] Stupid, stupid static!! I'm going to Target! We always have something going on here - static, heat and yes in certain months of the year we have humidity. Thanks for the humidifier idea. I'll spend the rest of the afternoon trying to decide if I want a frog or Hello Kitty. Cheers, Andi On Jan 5, 2011, at 1:26 PM, Emily Sours wrote: > Target! They have frogs too! I enjoy the elephant design the best. Well, > next to Hello Kitty, but that's not for everyone. > > *http://tinyurl.com/2frkw3r* > > Emily > > Writer Richard Suflet recommended drinking large doses of strong vinegar > with fleas to cure the illnesses that resulted from swallowing the > horse-leeches that were common in drinking water. > -Every Home a Distillery, Sarah Meacham > > > On Wed, Jan 5, 2011 at 3:00 PM, Rathborne, Toni < > trathborne@somerset-healthcare.com> wrote: > >> Where does one get a penguin? >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jshelley <@t> sanfordburnham.org Thu Jan 6 07:44:50 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Thu Jan 6 07:44:57 2011 Subject: [Histonet] RE: Research charges In-Reply-To: <04A71EFE05FE5F4DAA05D0500FD0597002A016DF@Distal.dentnet.dent.ohio-state.edu> References: <04A71EFE05FE5F4DAA05D0500FD0597002A016DF@Distal.dentnet.dent.ohio-state.edu> Message-ID: Hi Mary, I am a new core starting up and that would be helpful for me also. If you get any info could you past it my way also. Thanks!!! Kind Regards! ? John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Lloyd Sent: Thursday, January 06, 2011 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Research charges Would anyone like to share their charges for research. I am interested in these charges. Routine processing Hand processing H&E Unstained slides Routine Special Stains (PAS,GMS etc.) I would also like to know policies on charging for repeat work. If a researcher is interested in an area(bone) that is difficult and needs to be recut over and over again because of lifting and folding in the area of interest. After using many different techniques I have resolved the problem but some researchers demand more and more for free. Also I have a couple of researchers that stand with me while I am cutting through the block to keep the lesional slides only in their blocks. It takes around 45 minutes per block. Is their any compensation for the time I spend with the researcher. I put through some new policies and prices but my pathologist says I need documentation of those charges. Thanks Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sharon.Davis-Devine <@t> carle.com Thu Jan 6 08:29:15 2011 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Thu Jan 6 08:29:19 2011 Subject: [Histonet] Controls needed Message-ID: Does anyone out there in Histoland have a control block for MDM2 for liposarcoma that they would be willing to share? We don't get very many liposarcomas so control blocks are a problem. Any help is greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From ttruscot <@t> vetmed.wsu.edu Thu Jan 6 10:24:04 2011 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Jan 6 10:24:11 2011 Subject: [Histonet] Slide Labeling System that Survives Citrate Boiling In-Reply-To: References: Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB401476E77D1B4@CVMMBX.vetmed.wsu.edu> That sounds like the one TBS made or makes. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Wednesday, January 05, 2011 10:39 PM To: Cameron Nowell; Histonet Subject: Re: [Histonet] Slide Labeling System that Survives Citrate Boiling long ago there was a cute system that worked with superfrost type slides - you know the ones that have the sort of coloured ends - etched through the paint to make a permanent marking Common HB pencil also survives on ordinary frosted slidesand surgipath pen marking esp if used on superfrost slides On 1/6/11, Cameron Nowell wrote: > Hi List, > > > > I am looking for a slide labelling system to print labels for our > research histology samples that will survive pretty much anything we > throw at it. There seems to be lots of choices out there for chemical > resistant labels but i can't seem to find much on ones that are > resistant to antigen retrieval like citrate boiling. I have searched > google and the histonet archives and the best i can find is a reference > to General Data having some that may do the job. Does anyone out there > have any more info or are you using someting that works well? > > > > Thanks > > > > Cam > > > > > > > > Cameron J. Nowell > Microscopy Manager > Centre for Advanced Microscopy > Ludwig Institute for Cancer Research > PO Box 2008 > Royal Melbourne Hospital > Victoria, 3050 > AUSTRALIA > > Office: +61 3 9341 3155 > Mobile: +61422882700 > Fax: +61 3 9341 3104 > > Facility Website > > > > > > > > This communication is intended only for the named recipient and may contain > information that is confidential, legally privileged or subject to > copyright; the Ludwig Institute for Cancer Research Ltd does not waive any > rights if you have received this communication in error. > The views expressed in this communication are those of the sender and do not > necessarily reflect the views of the Ludwig Institute for Cancer Research > Ltd. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> wvhcs.org Thu Jan 6 10:40:24 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Thu Jan 6 10:43:21 2011 Subject: [Histonet] Re: Pax-2 References: <201101051710.p05HAI5e027394@node0gw.wvhcs.org> Message-ID: Pax-2 (rabbit poly) is available from Cell Marque but in our experience it also has some cytoplasmic background staining. Mike Dessoye ----------- Message: 21 Date: Tue, 4 Jan 2011 16:06:56 -0800 From: Mark Tarango Subject: [Histonet] Pax-2 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonet, My lab needs to switch vendors on this antibody. Where is everyone buying Pax-2? I found on their website that Invitrogen sells an IVD rabbit poly Pax-2 antibody. Has anyone tried this or others? What about Epitomics RUO rabbit mono? Do all the antibodies give that cytoplasmic background staining that I've seen? I'd appreciate any info at all. Thanks Mark Tarango _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From mjdessoye <@t> wvhcs.org Thu Jan 6 10:44:05 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Thu Jan 6 10:46:46 2011 Subject: [Histonet] RE: Lab Chairs References: <201101051801.p05I1cXI010205@node0gw.wvhcs.org> Message-ID: We have office and lab chairs from Haworth. They're very expensive, with ours around $800, but they have a lifetime warranty. We just had two sent for repair or replacement that were 10+ years old and there was no problem. Mike Dessoye ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 Jan 2011 11:17:26 -0600 From: Jay Lundgren Subject: Re: [Histonet] Lab chairs To: Liz Chlipala Cc: histonet@lists.utsouthwestern.edu, "Sharon.Davis-Devine" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Good luck finding good task chairs that don't cost a fortune. The good ones are all very expensive now days. I think that buying cheap ones is a false savings, however, as they will fall apart in short order. I have personally seen a busy histo lab destroy new (cheap) chairs in a year. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 5 Jan 2011 09:26:46 -0800 (PST) From: Sheila Haas Subject: Re: [Histonet] Lab chairs To: Jay Lundgren , Liz Chlipala Cc: histonet@lists.utsouthwestern.edu, "Sharon.Davis-Devine" Message-ID: <967402.42392.qm@web57806.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I have to agree with Jay. A couple of years ago, we purchased new, expensive lab chairs (from an office supply store that customized) and they look great even now. I think it's worth the money from an ergonomic standpoint and also because they hold up much better. Just my 2 cents! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ________________________________ From: Jay Lundgren To: Liz Chlipala Cc: histonet@lists.utsouthwestern.edu; Sharon.Davis-Devine Sent: Wed, January 5, 2011 12:17:26 PM Subject: Re: [Histonet] Lab chairs Good luck finding good task chairs that don't cost a fortune. The good ones are all very expensive now days. I think that buying cheap ones is a false savings, however, as they will fall apart in short order. I have personally seen a busy histo lab destroy new (cheap) chairs in a year. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 5 Jan 2011 12:49:54 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] inventory To: Kelly Boyd , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640814F91642@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" We do it annually. We use a combination of reports from our Materials Management Dept. and from our order spread sheet. (I keep a spread sheet of all items ordered). It's not totally accurate because of the chemicals and stains that have been here forever, but it's close enough for Mat Mgt. Best, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Wednesday, January 05, 2011 12:08 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inventory Yesterday I inquired If and how labs did inventory for specials and immunos. I meant to ask IF and HOW labs do end of year inventory for specials and immunos. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 86, Issue 7 *************************************** _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From marktarango <@t> gmail.com Thu Jan 6 11:09:34 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jan 6 11:09:41 2011 Subject: [Histonet] Re: Pax-2 In-Reply-To: References: <201101051710.p05HAI5e027394@node0gw.wvhcs.org> Message-ID: Thanks for the response. I heard Cell Marque's rabbit died and they aren't shipping any more ab until they get a new supplier. If anyone is using an antibody from someone other than Cell Marque, I'd be interested. Thanks Mark On Thu, Jan 6, 2011 at 8:40 AM, Dessoye, Michael J wrote: > Pax-2 (rabbit poly) is available from Cell Marque but in our experience it > also has some cytoplasmic background staining. > > Mike Dessoye > > ----------- > Message: 21 > Date: Tue, 4 Jan 2011 16:06:56 -0800 > From: Mark Tarango > Subject: [Histonet] Pax-2 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Histonet, > > My lab needs to switch vendors on this antibody. Where is everyone buying > Pax-2? I found on their website that Invitrogen sells an IVD rabbit poly > Pax-2 antibody. Has anyone tried this or others? What about Epitomics RUO > rabbit mono? Do all the antibodies give that cytoplasmic background > staining that I've seen? > > I'd appreciate any info at all. > > Thanks > > Mark Tarango > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Wyoming Valley Health Care System. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From JWeems <@t> sjha.org Thu Jan 6 11:22:09 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 6 11:22:13 2011 Subject: [Histonet] Billing question Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640814F9183E@CHEXCMS10.one.ads.che.org> When you have performed a special stain or immuno that does not help the pathologist (tumor exhausted, etc) do you still charge for the technical component and the pathologist credits the professional fee? Just curious.. Happy New Year, Everyone! Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From 41dmb41 <@t> gmail.com Thu Jan 6 11:39:44 2011 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Thu Jan 6 11:42:44 2011 Subject: [Histonet] Billing question In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640814F9183E@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640814F9183E@CHEXCMS10.one.ads.che.org> Message-ID: Hey Joyce, My understanding has always been that if the pathologist specifically mentions the stain in the report, then it's OK to charge all the fees. However, if the pathologist chooses to leave any mention of the stain/immuno in the report, then you can't bill for it. So if the pathologist mentions a specific stain was done, but that the findings were negative, you can still charge. In the case of an exhausted tumor, I think the ultimate discretion comes to whether or not the pathologist chooses to mention it in the report. That's just from my experience, but I would love to hear how others are handling instances such as these, too! Drew On Thu, Jan 6, 2011 at 12:22, Weems, Joyce wrote: > When you have performed a special stain or immuno that does not help the > pathologist (tumor exhausted, etc) do you still charge for the technical > component and the pathologist credits the professional fee? Just curious.. > > Happy New Year, Everyone! > > Thanks! j > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hhawkins <@t> UTMB.EDU Thu Jan 6 11:43:22 2011 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Thu Jan 6 11:44:50 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: References: <4f0ed.366457b5.3a566926@aol.com>, Message-ID: <18DADD3ED840AC46AB24616A927CD0DB1E24A34316@EXCHANGE2.utmb.edu> There are devices used in cryo-ultramicrotomy that might help. I have no experience myself. Also, I found this interesting article via Google. http://www.diatome.ch/en/products/staticlineii.asp http://jhc.sagepub.com/content/37/7/1157.full.pdf ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours [talulahgosh@gmail.com] Sent: Wednesday, January 05, 2011 8:24 PM To: Gervaip@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Stupid, stupid static!! You know, all of it sounds so easy...unless you're cryosectioning!! Does putting a humidifier outside help with static in a cryostat? Because the static is driving me nuts. Why aren't there teams of people working on this issue? It keeps coming up, yet there is no one good answer, Calling all engineers!! Emily Writer Richard Suflet recommended drinking large doses of strong vinegar with fleas to cure the illnesses that resulted from swallowing the horse-leeches that were common in drinking water. -Every Home a Distillery, Sarah Meacham On Wed, Jan 5, 2011 at 7:39 PM, wrote: > wipe the microtome and knife holder with a Bounce laundry softener sheet. > It works for us. > > > In a message dated 1/5/2011 3:38:19 P.M. Central Standard Time, > sbreeden@nmda.nmsu.edu writes: > > This has SO many possible responses but I'll not go there. Someone > might videotape it and leak it to the press... > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jan 6 12:01:38 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jan 6 12:01:42 2011 Subject: [Histonet] Research charges - long reply In-Reply-To: <04A71EFE05FE5F4DAA05D0500FD0597002A016DF@Distal.dentnet.dent.ohio-state.edu> Message-ID: Mary Mary We are a private contract lab and do not charge for recuts or repeats that are related to quality issues. We run a lot of bone samples through our lab and if there is a fold in the lesion area we recut the block at no charge to the client. All of our slides are QC'd and we will repeat anything we think we need to prior to shipping the study back to the client. If the client is not happy with the work, (which happens rarely) we will repeat at no cost. We are a quality lab that stands by that so we need to follow all of these steps. I feel it helps us maintain our clients, our clients know that we have their best interest in mind and that we are honest with them. We work with them all of the time to develop grossing, processing, sectioning and staining protocols that are specific to their tissues. Some of our basic charges to process and stain one block with H&E we charge $16.00 to $20.00, for hand processing we charge $25.00 per block and that includes an H&E, for unstained slides on plus slides $4.50 specials range from $16.50 to $29.50 depending upon the type of special. A lot of the tissues we work with need to be sectioned to a specific area, for example on mouse knees we need to section to the center of the knee joint, or with mouse or rat eyes we need to section in the area of the optic nerve head. All of it is very detail orientated. You can sometimes tell if you are in the correct area by just looking at the tissue in the block, then we would section, stain and review, if we were not in the correct area we would section again, stain and review. This took us a lot of time and we never would charge for those additional sections that we took. What we do now is that we have nice student microscopes (leica we spent around $1200.00 for each of them) next to each microtome so as the techs cut into the block they pick up and can review the unstained sections, this has helped us out a lot. We have less recuts now. The samples that we need to fiddle with a bit on trimming are the ones that we charge $20.00 per block, like bone and eyes. For those individuals who want to stand by you while you section through their block and only collect the lesions I would either charge them an hourly rate or state that they have to pay for each unstained slide and stained slide - you should be doing this already. Researchers sometimes have limited budgets, but they should be paying for what you provide to them. You have a budget that you have to work with, and they need to be aware of that, your time and supplies cost money too. You can work deals out, like having them pay for supplies for their projects. You sometimes need to get creative. I hope this helps Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Lloyd Sent: Thursday, January 06, 2011 4:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Research charges Would anyone like to share their charges for research. I am interested in these charges. Routine processing Hand processing H&E Unstained slides Routine Special Stains (PAS,GMS etc.) I would also like to know policies on charging for repeat work. If a researcher is interested in an area(bone) that is difficult and needs to be recut over and over again because of lifting and folding in the area of interest. After using many different techniques I have resolved the problem but some researchers demand more and more for free. Also I have a couple of researchers that stand with me while I am cutting through the block to keep the lesional slides only in their blocks. It takes around 45 minutes per block. Is their any compensation for the time I spend with the researcher. I put through some new policies and prices but my pathologist says I need documentation of those charges. Thanks Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adam.smith <@t> adeccona.com Thu Jan 6 12:10:25 2011 From: adam.smith <@t> adeccona.com (Smith, Adam) Date: Thu Jan 6 12:10:42 2011 Subject: [Histonet] Job opening for a Histology Manager at HealthTronics in Augusta, GA 30901 Message-ID: <0980CC29978E784588667491783476B80A54F3BE53@MEUSITINFEVS02.am.adecco.net> Hello, my name is Adam, I am a recruiter at Adecco Medical & Science and I have an opening for a Histology Manager in Augusta, GA 30901. This position will fill quickly so if you are interested please reach out to me as soon as you can. I have listed the job description and required qualifications about the position below. If you are not interested please feel free to pass this email along to anyone who may like to apply for this position. I look forward to speaking with you Please send me 2 - 3 professional references and an updated resume Job Details: Position title: Histology Manager Start date: ASAP Duration: Direct Hire Location: Augusta, GA 30901 Hours: 1st shift Job Details Reports to the Director of Laboratory Operations and is responsible for coordinating and directing activities of assigned section and subordinates engaged in performing routine and non-routine histology procedures. In doing so, ensures quality and quantity standards relating to the delivery of laboratory services are achieved by assuring results. -Knowledge of Science and Mathematics normally acquired through completion of an Associate's Degree. -Active/current certification as a Histotechnologist (HT or HTL) by the Board of Registry of the American Society of Clinical Pathologists is required. -Five years experience as an HTIII to include 2 years of management experience or 3-5 years experience as an HTIII and completion of internal manager training program, or equivalent work experience in a pathology lab setting. SUMMARY OF PURPOSE: Reports to the Laboratory Manager and is responsible for coordinating and directing activities of assigned section and subordinates engaged in performing routine and non-routine histology procedures. In doing so, ensures quality and quantity standards relating to the delivery of laboratory services are achieved by assuring results. ESSENTIAL FUNCTIONS: 1. In collaboration with the Laboratory Manager plans, coordinates, monitors and oversees work of assigned section and subordinate personnel engaged in performing histology procedures needed to obtain data for disease diagnosis and treatment. 2. Accomplishes, or effectively recommends the following: * Prepares schedules to assure adequate coverage. * Assigns work * Counsels employees on work or working relationships * Arranges and/or conducts job training, continuing education. * Approves time worked * Completes employee orientation for assigned section. * Interviews, hires, and evaluates the performance of and, when necessary, disciplines and discharges subordinate supervisory personnel. * Approves hiring and, when necessary, discharge recommendations of subordinate personnel and assists in resolving complex employee relations matters. 3. Ensures all technical procedures are performed correctly. Procedures with higher priority are completed first, equipment has proper maintenance performed and kept in good working order. 4. Reviews and evaluates new products, equipment. 5. Evaluates and implements new procedures as necessary. 6. Makes supply decisions with sufficient accuracy so no supplies run out or remains beyond the expiration date. 7. Responsible for daily quality control and quality assurance to include establishing training and maintaining Q.C. and Q.A protocol with subordinate staff. 8. Coordinates monthly QA and QC data. 9. Ensures that laboratory section meets all safety and other regulatory requirements. 10. Effectively manages technical aspects of hospital accounts 11. Gathers information and carries through to completion assigned projects while adhering to established time frames. 12. Prepares periodic reports as necessary. 13. Assures activities of assigned section are coordinated to insure quality patient care and economics of operation. 14. Participates in continuing education as required. 15. Consults with LIS Administrator to troubleshoot LIS problems and enhancements. 16. Participates in the development of assigned section and department wide policies and procedures. 17. Maintains all section records relating to operation such as quality control and productivity statistics, incident reports and the like. 18. Reviews and summarizes records and prepares reports for management review. 19. Develops and maintains cooperative working relationships with physicians and various other Healthtronics employees. 20. Provides advice and direction to subordinates for technical problems encountered. Explains and demonstrates appropriate techniques or methods as necessary. 21. Performs routine procedures in achieving workload demands. 22. Maintains knowledge of current trends and developments in the field of expertise. 23. Performs other duties as assigned POSITION REQUIREMENTS: Education and formal training: Knowledge of Science and Mathematics normally acquired through completion of an Associate's Degree. License: Active/current certification as a Histotechnologist (HT or HTL) by the Board of Registry of the American Society of Clinical Pathologists is required. Experience: 1. Five years experience as an HTIII to include 2 years of management experience or 3-5 years experience as an HTIII and completion of internal manager training program, or equivalent work experience in a pathology lab setting. 2. Analytical skills necessary in order to participate in planning of department activities provide input into purchase of equipment, and so forth. 3. Interpersonal skills necessary in order to coordinate activities of various units within the department, exchange information with vendors, and so forth. 4. Must be able to communicate effectively by telephone, e-mail and in person with customers, co-workers and supervisors. 5. Ability to apply concepts such as fractions, percentages, ratios, and proportions to practical situations. 6. Relies on experience and judgment to plan and accomplish goals. 7. A certain degree of creativity and latitude is expected. 8. Requires ability to handle irate customers, good judgment on tough decisions, and ability to resolve complex situations PHYSICAL/MENTAL REQUIREMENTS: 1. Occasional walking, standing, bending and reaching when filing, photocopying, etc. 2. Light lifting of under 50 pounds on an infrequent basis. 3. There is a moderate potential for skin, eye, mucous membrane, non-intact skin or parenteral contact with potentially infectious materials or chemicals may result from the performance of an employee's regular duties. 4. Repetitive wrist motion when performing microtomy procedures. Regards, Adam Smith Medical & Science recruiting specialist Adecco Medical & Science 2235 South Clinton Avenue Rochester, NY 14618 Office: 585.454.5511 Toll Free: 866.217.3454 Cell: 585.414.1869 Fax: 585.454.5519 adam.smith@adeccona.com Visit us @ www.adeccousa.com | www.ajilonconsulting.com | Apply Here Today! From Milton.Gomez <@t> nyumc.org Thu Jan 6 12:42:57 2011 From: Milton.Gomez <@t> nyumc.org (Gomez, Milton) Date: Thu Jan 6 12:45:24 2011 Subject: [Histonet] Hello Histonetters and Happy New Year! Message-ID: <4A53F9A1D7C2674FA4A6E650D703DDA5D403ED13@MSGWSDCPMB07.nyumc.org> Hello Histonetters and Happy New Year, Does anyone have a Validation Protocol template for immunohistochemical antibodies that would like to share? Thanks in advance, MG ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 6 13:25:44 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 6 13:25:53 2011 Subject: [Histonet] Billing question In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E1640814F9183E@CHEXCMS10.one.ads.che.org> Message-ID: We've been repeatedly told by Compliance that if a pathologist (or a clinician) orders a test, the test result must be included in the report and the test must be billed for; even if the result is non-contributory. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Thursday, January 06, 2011 12:40 PM To: Weems, Joyce Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing question Hey Joyce, My understanding has always been that if the pathologist specifically mentions the stain in the report, then it's OK to charge all the fees. However, if the pathologist chooses to leave any mention of the stain/immuno in the report, then you can't bill for it. So if the pathologist mentions a specific stain was done, but that the findings were negative, you can still charge. In the case of an exhausted tumor, I think the ultimate discretion comes to whether or not the pathologist chooses to mention it in the report. That's just from my experience, but I would love to hear how others are handling instances such as these, too! Drew On Thu, Jan 6, 2011 at 12:22, Weems, Joyce wrote: > When you have performed a special stain or immuno that does not help the > pathologist (tumor exhausted, etc) do you still charge for the technical > component and the pathologist credits the professional fee? Just curious.. > > Happy New Year, Everyone! > > Thanks! j > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From billodonnell <@t> catholichealth.net Thu Jan 6 15:05:07 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Jan 6 15:05:21 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: <18DADD3ED840AC46AB24616A927CD0DB1E24A34316@EXCHANGE2.utmb.edu> References: <4f0ed.366457b5.3a566926@aol.com>, <18DADD3ED840AC46AB24616A927CD0DB1E24A34316@EXCHANGE2.utmb.edu> Message-ID: Follow-up. The zero-stat gun seems to be around $150.00 most everywher. But it does work and will last for years. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hawkins, Hal K. Sent: Thursday, January 06, 2011 11:43 AM To: Emily Sours; Gervaip@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Stupid, stupid static!! There are devices used in cryo-ultramicrotomy that might help. I have no experience myself. Also, I found this interesting article via Google. http://www.diatome.ch/en/products/staticlineii.asp http://jhc.sagepub.com/content/37/7/1157.full.pdf ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours [talulahgosh@gmail.com] Sent: Wednesday, January 05, 2011 8:24 PM To: Gervaip@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Stupid, stupid static!! You know, all of it sounds so easy...unless you're cryosectioning!! Does putting a humidifier outside help with static in a cryostat? Because the static is driving me nuts. Why aren't there teams of people working on this issue? It keeps coming up, yet there is no one good answer, Calling all engineers!! Emily Writer Richard Suflet recommended drinking large doses of strong vinegar with fleas to cure the illnesses that resulted from swallowing the horse-leeches that were common in drinking water. -Every Home a Distillery, Sarah Meacham On Wed, Jan 5, 2011 at 7:39 PM, wrote: > wipe the microtome and knife holder with a Bounce laundry softener sheet. > It works for us. > > > In a message dated 1/5/2011 3:38:19 P.M. Central Standard Time, > sbreeden@nmda.nmsu.edu writes: > > This has SO many possible responses but I'll not go there. Someone > might videotape it and leak it to the press... > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Thu Jan 6 15:05:25 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Jan 6 15:05:41 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: <18DADD3ED840AC46AB24616A927CD0DB1E24A34316@EXCHANGE2.utmb.edu> References: <4f0ed.366457b5.3a566926@aol.com>, <18DADD3ED840AC46AB24616A927CD0DB1E24A34316@EXCHANGE2.utmb.edu> Message-ID: I have used the product linked below (of course mine was purchased at a vinyl record store for neutralizing static on turn-tables. It cost a whopping $15.00 at the time. Maybe some folks can still get them there? http://www.sigmaaldrich.com/catalog/ProductDetail.do?D7=0&N5=SEARCH_CONC AT_PNO%7CBRAND_KEY&N4=Z108812%7CALDRICH&N25=0&QS=ON&F=SPEC William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hawkins, Hal K. Sent: Thursday, January 06, 2011 11:43 AM To: Emily Sours; Gervaip@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Stupid, stupid static!! There are devices used in cryo-ultramicrotomy that might help. I have no experience myself. Also, I found this interesting article via Google. http://www.diatome.ch/en/products/staticlineii.asp http://jhc.sagepub.com/content/37/7/1157.full.pdf ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours [talulahgosh@gmail.com] Sent: Wednesday, January 05, 2011 8:24 PM To: Gervaip@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Stupid, stupid static!! You know, all of it sounds so easy...unless you're cryosectioning!! Does putting a humidifier outside help with static in a cryostat? Because the static is driving me nuts. Why aren't there teams of people working on this issue? It keeps coming up, yet there is no one good answer, Calling all engineers!! Emily Writer Richard Suflet recommended drinking large doses of strong vinegar with fleas to cure the illnesses that resulted from swallowing the horse-leeches that were common in drinking water. -Every Home a Distillery, Sarah Meacham On Wed, Jan 5, 2011 at 7:39 PM, wrote: > wipe the microtome and knife holder with a Bounce laundry softener sheet. > It works for us. > > > In a message dated 1/5/2011 3:38:19 P.M. Central Standard Time, > sbreeden@nmda.nmsu.edu writes: > > This has SO many possible responses but I'll not go there. Someone > might videotape it and leak it to the press... > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nacaela.Johnson <@t> USONCOLOGY.COM Thu Jan 6 15:36:15 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Thu Jan 6 15:37:20 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: References: Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D487@txhous1eb012.uson.usoncology.int> I am also having issues with static, but only on my particle blocks. This happens in the summer as well. The static is actually "in" the block. The core biopsies cut wonderfully. Does anyone else run into this problem? I can see the effects of the static when I'm embedding, the particles float to the edges of the mold instead of in a clump. Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, January 05, 2011 1:15 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From sbreeden <@t> nmda.nmsu.edu Thu Jan 6 15:45:21 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jan 6 15:45:25 2011 Subject: [Histonet] Static issues Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47565@nmdamailsvr.nmda.ad.nmsu.edu> This is New Mexico where "humidity" is a rumor. The humidity in the lab here - as I write - is 18% and that's on a really wet day! If I have static issues with my ribbons, I just lean a little bit toward the block and "breathe" on it and the ribbons just float (in a good way) off the knife. I do that so often that when I use my sewing machine, I find myself breathing on the material. That's just sad! But try the Breathing Thing. Or not. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From JWeems <@t> sjha.org Thu Jan 6 15:50:27 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 6 15:50:32 2011 Subject: [Histonet] RE: Static issues In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47565@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47565@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640814F91931@CHEXCMS10.one.ads.che.org> I was going to say the same thing... Just hadn't taken time. So may of us are full of hot air.. J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, January 06, 2011 16:45 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Static issues This is New Mexico where "humidity" is a rumor. The humidity in the lab here - as I write - is 18% and that's on a really wet day! If I have static issues with my ribbons, I just lean a little bit toward the block and "breathe" on it and the ribbons just float (in a good way) off the knife. I do that so often that when I use my sewing machine, I find myself breathing on the material. That's just sad! But try the Breathing Thing. Or not. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sgoebel <@t> mirnarx.com Thu Jan 6 16:01:24 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Jan 6 16:01:28 2011 Subject: [Histonet] Static issues In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47565@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47565@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Breathing is my normal way to attack static, it's not a problem getting the ribbon...there is so much static that when I pull it off the tome, it literally sucks to my hand like a magnet...and then it's gone. Thanks everyone for your suggestions! I am hoping to only have to deal with this for a short while longer. I like the penguin idea just cause I think that will be the funniest!! Thanks again!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, January 06, 2011 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Static issues This is New Mexico where "humidity" is a rumor. The humidity in the lab here - as I write - is 18% and that's on a really wet day! If I have static issues with my ribbons, I just lean a little bit toward the block and "breathe" on it and the ribbons just float (in a good way) off the knife. I do that so often that when I use my sewing machine, I find myself breathing on the material. That's just sad! But try the Breathing Thing. Or not. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Jan 6 16:07:20 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 6 16:07:28 2011 Subject: [Histonet] Static issues In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B02E47565@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640814F91935@CHEXCMS10.one.ads.che.org> Breathing is for the cryostat sections. Dryer sheets for the paraffin sections.. It also helps to run screaming through the lab... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Thursday, January 06, 2011 17:01 To: sbreeden@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Static issues Breathing is my normal way to attack static, it's not a problem getting the ribbon...there is so much static that when I pull it off the tome, it literally sucks to my hand like a magnet...and then it's gone. Thanks everyone for your suggestions! I am hoping to only have to deal with this for a short while longer. I like the penguin idea just cause I think that will be the funniest!! Thanks again!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, January 06, 2011 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Static issues This is New Mexico where "humidity" is a rumor. The humidity in the lab here - as I write - is 18% and that's on a really wet day! If I have static issues with my ribbons, I just lean a little bit toward the block and "breathe" on it and the ribbons just float (in a good way) off the knife. I do that so often that when I use my sewing machine, I find myself breathing on the material. That's just sad! But try the Breathing Thing. Or not. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From hfedor <@t> jhmi.edu Thu Jan 6 16:43:14 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Jan 6 16:43:19 2011 Subject: [Histonet] Charged slides Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D31785B5BE0D@JHEMTEXVS3.win.ad.jhu.edu> Hi, A question has come up regarding the different methods used to put a charge on the slide. We recently ordered some plus slides and the boxes they are packed in say silanized slides, but they say "plus" on the slide . We don't want to use these for our clients if they are not going to be getting the same results as the former "plus" slides that we were using. I was under the delusion that Plus slides somehow are magically charged without any coating process taking place. So does anyone know exactly how a "plus" slide gets its charge? Do they all get dipped in APES, or Polylysine? Thanks for your help. -- Helen From mab70 <@t> medschl.cam.ac.uk Fri Jan 7 02:33:33 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Jan 7 02:35:56 2011 Subject: [Histonet] RE: Static issues In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640814F91931@CHEXCMS10.one.ads.che.org> References: <4D14F0FC9316DD41972D5F03C070908B02E47565@nmdamailsvr.nmda.ad.nmsu.edu> <92AD9B20A6C38C4587A9FEBE3A30E1640814F91931@CHEXCMS10.one.ads.che.org> Message-ID: I live in moist old UK and have always breathed on my ribbons, can't get out of the habit, but do remember to breath and not too heavily! On the cryostat front, I used to suffer from static terribly and tried all sorts of things, but since using disposable blades have had fewer problems. I just move the blade along or replace it and it often cures the problem. Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: 06 January 2011 21:50 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Static issues I was going to say the same thing... Just hadn't taken time. So may of us are full of hot air.. J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, January 06, 2011 16:45 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Static issues This is New Mexico where "humidity" is a rumor. The humidity in the lab here - as I write - is 18% and that's on a really wet day! If I have static issues with my ribbons, I just lean a little bit toward the block and "breathe" on it and the ribbons just float (in a good way) off the knife. I do that so often that when I use my sewing machine, I find myself breathing on the material. That's just sad! But try the Breathing Thing. Or not. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Fri Jan 7 05:36:18 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Jan 7 05:37:22 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: <6DBD71C31D7E444482E5D3DFBC202D260245D487@txhous1eb012.uson.usoncology.int> References: <6DBD71C31D7E444482E5D3DFBC202D260245D487@txhous1eb012.uson.usoncology.int> Message-ID: <4317AC71-7D4F-47A7-8E10-5C46E873B4CB@imagesbyhopper.com> I have a tech who complains about static/compression in our cell blocks, but I don't seem to have the same problem as her. The difference? I face my blocks and then soak it on my waterbath for about 15 -30 seconds and then it goes onto the ice tray. The additional moisture that is soaked up in the block seems to correct the issue for me. Sort of like breathing heavily on the block! Michelle On Jan 6, 2011, at 4:36 PM, "Johnson, Nacaela" wrote: > I am also having issues with static, but only on my particle blocks. > This happens in the summer as well. The static is actually "in" the > block. The core biopsies cut wonderfully. Does anyone else run into > this problem? I can see the effects of the static when I'm embedding, > the particles float to the edges of the mold instead of in a clump. > > > Thanks, > > Nacaela Johnson > Histology Technician > KCCC Pathology > 12000 110th St., Ste. 400 > Overland Park, KS 66210 > Office: 913-234-0576 > Fax: 913-433-7639 > Email: Nacaela.Johnson@USOncology.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > sgoebel@mirnarx.com > Sent: Wednesday, January 05, 2011 1:15 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Stupid, stupid static!! > > So where the microtome is here that I have to use we have to wear those > blue hospital booties and disposable lab coats (the white paper type > ones). With me and several other people walking around in those booties > the amount of static electricity is to say the least frusterating!! > Does anyone know of anything I can do to get rid of the static? > > Thanks > > > > Sarah Goebel, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren <@t> gmail.com Fri Jan 7 07:03:27 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Jan 7 07:03:33 2011 Subject: [Histonet] Stupid, stupid static!! In-Reply-To: <4317AC71-7D4F-47A7-8E10-5C46E873B4CB@imagesbyhopper.com> References: <6DBD71C31D7E444482E5D3DFBC202D260245D487@txhous1eb012.uson.usoncology.int> <4317AC71-7D4F-47A7-8E10-5C46E873B4CB@imagesbyhopper.com> Message-ID: They use static guard spray here at this facility in IL, but it does make it smell like your matronly aunt just walked into the room. Jay A. Lundgren M.S., HTL (ASCP) > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Fri Jan 7 07:56:18 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Jan 7 07:56:24 2011 Subject: [Histonet] isosulfan blue for rodent lymph node dissection Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D76C8B77@usctmx1176.merck.com> Happy New Year all - Has anyone had experience with injecting isosulfan-blue sub-q into rats/mice priori to euthanizing for coloring of lymph nodes to make them easier to find ?? Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From alisha <@t> ka-recruiting.com Fri Jan 7 09:37:57 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Fri Jan 7 09:37:55 2011 Subject: [Histonet] Great histology jobs in Maine Message-ID: <136081830.1294414677074.JavaMail.cfservice@SL4APP1> Hi Histonet Members, I am a healthcare recruiter, specifically focused on the laboratory/pathology space. I have openings all across the country and would be interested in speaking with anyone interested in searching for a new job. Below is a great new opportunity that I am working on: Histotech and Histology Supervisor Opening in Maine: I am currently working on an amazing opportunity with one of largest most sophisticated labs in the Northeast. It is located in coastal Maine. This company is looking to hire on a Histology Supervisor and a Histotech. They currently are looking for someone with a Bachelor's Degree, histology experience, HTL(ASCP) or HT(ASCP) certified, and supervisory experience for the supervisor position. The compensation package is fantastic and includes health, dental, a retirement plan, and relocation assistance, if needed. If interested in learning more details, please email me at alisha@ka-recruiting.com. If you are not interested but know of someone you could recommend for this position, please pass on their contact information to me. We have a referral bonus for anyone you refer to me that I place into a new job. I am also working on the following histology/pathology jobs: * Technical Support Specialist - Western territory (need PA or HTL license) * Technical Support Specialist - PA/NY territory (need PA or HTL license) * Technical Support Specialist - Southeastern territory (need PA or HTL license) * MI - Dermpath Histology Manager * OK - Histology Supervisor * NV - Histotechnologist 3rd shift * NY - Long Island - Dermpath Lab Manager * NH - Histology Supervisor * Eastern OH - Histology Supervisor * Maine - Histotechnologist * Maine - Histology Supervisor * FL - Histotechnologist * NY City - Histology Supervisor with IHC experience * NY City - Histotechnologist * NY City - Grossing Tech * NV - Pathologist's Assistant If interested in any of the opportunities above, please email me. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From jconnelly <@t> sleh.com Fri Jan 7 10:04:04 2011 From: jconnelly <@t> sleh.com (Connelly, John) Date: Fri Jan 7 10:04:16 2011 Subject: [Histonet] re: Pax-2 Message-ID: <0DD7BD0E1F64864A8982BE7EC71492BF0500E945@NTMS9.sleh.com> We have been using the invitrogen AB. It does not have much background and works well. John Connelly, M.D. "Message: 21 Date: Tue, 4 Jan 2011 16:06:56 -0800 From: Mark Tarango > Subject: [Histonet] Pax-2 To: "histonet@lists.utsouthwestern.edu" > Message-ID: > Content-Type: text/plain; charset=ISO-8859-1 Hi Histonet, My lab needs to switch vendors on this antibody. Where is everyone buying Pax-2? I found on their website that Invitrogen sells an IVD rabbit poly Pax-2 antibody. Has anyone tried this or others? What about Epitomics RUO rabbit mono? Do all the antibodies give that cytoplasmic background staining that I've seen? I'd appreciate any info at all. Thanks Mark Tarango" +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From anonwums1 <@t> gmail.com Fri Jan 7 11:18:07 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Jan 7 11:19:15 2011 Subject: [Histonet] Toluidine blue staining of CFU-F Message-ID: Hi all, I'm looking for a protocol to counterstain mouse CFU-F grown in tissue culture**. The protocols I've found just say they counterstained with this dye, don't say what it's dissolved in, sometimes list the percentage of it, and never list the time they stained for. The only full protocol I found was for staining of mast cells in sections, but that's dissolved in 70% ethanol and very acidified. I'm not sure that's what I want. Has anyone done this before? Thanks, Adam From histo20 <@t> hotmail.com Fri Jan 7 11:45:35 2011 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Fri Jan 7 11:45:39 2011 Subject: [Histonet] Lost Blocks Message-ID: HI all, Does anyone have a procedure outlining checks and balances to assure blocks have been placed on the processor, blocks that are missing, either before or after embedding, etc. Any help would be greatly appreciated! Thank you, Paula Wilder St. Joseph Medical Center Towson, MD 21204 From tjay30 <@t> yahoo.com Fri Jan 7 12:50:59 2011 From: tjay30 <@t> yahoo.com (Timothy Jay) Date: Fri Jan 7 12:51:03 2011 Subject: [Histonet] Job Opening in Torrance, CA Message-ID: <841166.48231.qm@web34308.mail.mud.yahoo.com> Histotech needed in Torrance, CA for a brand new path lab in a 6 physician GI practice. Full autonomy, great working conditions, excellent pay and benefits. Must be licensed in the State of California and have all necessary education and training to work independently. Send resumes to tjay30@yahoo.com. ? Thank you. ? Tim From sgoebel <@t> mirnarx.com Fri Jan 7 13:26:49 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Jan 7 13:26:54 2011 Subject: [Histonet] Cytology blender Message-ID: Hey all, At an old job we had this tiny little blender with a kind of diaphragm top on it to blend sputum cytology specimens with. It probably only held about 200cc of fluid at the most. Does anyone have a clue where I can find one of these? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From c.tague <@t> pathologyarts.com Fri Jan 7 13:37:21 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Fri Jan 7 13:37:25 2011 Subject: [Histonet] control block swap Message-ID: <003a01cbaea2$4d42a7a0$e7c7f6e0$@tague@pathologyarts.com> I have a few good WT-1 control blocks I'm willing to barter with, any takers? Curt Tague CEO, Pathology Arts From NHeath <@t> Lifespan.org Fri Jan 7 14:18:26 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Fri Jan 7 14:18:53 2011 Subject: [Histonet] ArrayMold System Message-ID: <130E8991F210424096EFC6F42EA33B24071EC131@LSCOEXCH1.lsmaster.lifespan.org> Wondering if anyone has used/purchased the ArrayMold "tissue micro array system"?? Positive and negative feedback would be greatly appreciated :) Nancy Heath, HT(ASCP) March 10, 2011 is Histotechnology Professionals Day From foreightl <@t> gmail.com Fri Jan 7 14:25:48 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Jan 7 14:25:53 2011 Subject: [Histonet] Lost Blocks In-Reply-To: References: Message-ID: Here we take a picture of the blocks before they go on the processor. We have a barcoding system that lets me know who embedded what and when. With over 140,000 blocks/year we only have had one instance where a block was lost between grossing and embedding. Because we have some off-site pathologists and grossing staff, our lab relies very heavily on tracking every specimen that comes in, every block that goes to histology and every stained slide that goes to our pathologists. Good luck, it can be frustrating when you lose a block. On Fri, Jan 7, 2011 at 9:45 AM, Paula Wilder wrote: > > HI all, > > Does anyone have a procedure outlining checks and balances to assure blocks > have been placed on the processor, blocks that are missing, either before or > after embedding, etc. Any help would be greatly appreciated! > Thank you, > > Paula Wilder > St. Joseph Medical Center > Towson, MD 21204 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From liz <@t> premierlab.com Fri Jan 7 15:00:26 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jan 7 15:00:31 2011 Subject: [Histonet] Feulgen Stain for DNA Message-ID: Hello Everyone I have tried to find this answer on the web and in references but I need some help. With respects to the Feulgen stain, how sensitive is it? Can it detect smaller fragments of DNA? Does it have to be double stranded or will single stranded stain as well? Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From LStadler <@t> cbiolabs.com Fri Jan 7 15:08:34 2011 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Fri Jan 7 15:08:44 2011 Subject: [Histonet] VIP 1000 In-Reply-To: <20101223130114.D5D7F854A6@barracuda.cbiolabs.com> References: <20101223130114.D5D7F854A6@barracuda.cbiolabs.com> Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC08C726@cbiolabs05.CBiolabs.local> Are you looking for the VIP 1000 User Manual? If so, I can send a copy Lyn Stadler Senior Research Associate - Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, December 23, 2010 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 85, Issue 21 Importance: Low Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Kathleen Dittrich, where are you? (Settembre, Dana) 2. Physician signature (Webb, Dorothy L) 3. RE: Physician signature (Weems, Joyce) 4. Thomas Crowell is out of the office. (thomas.crowell@novartis.com) 5. RE: RE: Physician signature (Mike Pence) 6. RE: RE: Physician signature (Weems, Joyce) 7. High Paying Job Opportunity - Live anywhere (Alisha Dynan) 8. Fisher MicroProbe manual staining system (Martha Ward) 9. RE: RE: Physician signature (Morken, Tim) 10. Frozen Specimen Handling (John Shelley) 11. RE: RE: Physician signature (Weems, Joyce) 12. Symphony cover slipper (Clare Thornton) 13. VIP 1000 Help (Drew Meyer) 14. FW: ASCP Action Alert - Physician Signature Requirement _FYI (Liz Chlipala) 15. RE: Frozen Specimen Handling (sgoebel@mirnarx.com) 16. Used embedding center and microtome (alineumann@aol.com) 17. CAP Question regarding procedure manual (Victor Tobias) 18. RE: CAP Question regarding procedure manual (Weems, Joyce) 19. RE: CAP Question regarding procedure manual (WILLIAM DESALVO) 20. RE: CAP Question regarding procedure manual (Jesus Ellin) 21. Re: CAP Question regarding procedure manual (BSullivan@shorememorial.org) ---------------------------------------------------------------------- Message: 1 Date: Wed, 22 Dec 2010 13:18:22 -0500 From: "Settembre, Dana" Subject: [Histonet] Kathleen Dittrich, where are you? To: "Settembre, Dana" , 'IRENA SREBOTNIK KIRBIS' , "hfedor@jhmi.edu" , "ahutton@dh.org" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Kathleen Dittrich, formerly from Dako. Where are you? Dana Settembre ------------------------------ Message: 2 Date: Wed, 22 Dec 2010 12:36:43 -0600 From: "Webb, Dorothy L" Subject: [Histonet] Physician signature To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43EA95058335@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 3 Date: Wed, 22 Dec 2010 13:38:30 -0500 From: "Weems, Joyce" Subject: [Histonet] RE: Physician signature To: "Webb, Dorothy L" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B22@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Wed, 22 Dec 2010 14:01:42 -0500 From: thomas.crowell@novartis.com Subject: [Histonet] Thomas Crowell is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 12/22/2010 and will not return until 01/03/2011. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. ------------------------------ Message: 5 Date: Wed, 22 Dec 2010 13:25:20 -0600 From: "Mike Pence" Subject: RE: [Histonet] RE: Physician signature To: "Weems, Joyce" , "Webb, Dorothy L" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974AD6@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" And where would one go to see this proposal? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 12:39 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Physician signature It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 22 Dec 2010 14:47:42 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] RE: Physician signature To: Mike Pence , "Webb, Dorothy L" , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B65@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" The Federal Register is always a good place to find interesting reading. http://edocket.access.gpo.gov/2010/pdf/2010-15900.pdf Search for signature and you can find it...starts on page 40161. In the middle column on page 40162 charges for TC for AP are mentioned. consistent with the principle in ? 410.32(a) that a written order for diagnostic tests including those paid under the CLFS and those that are not paid under the CLFS (for example, that are paid under the PFS or under the OPPS), such as X-rays, MRIs, and the TC of physician pathology services, must be signed by the ordering physician or NPP. That is, the policy that signatures are not required on requisitions for clinical diagnostic laboratory tests paid based on the CLFS applies only to requisitions (as opposed to written orders) (74 FR 33642). Blah blah blah.... At the end they say "We believe that this policy would result in a less confusing process." Merry Christmas, Everyone! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, December 22, 2010 14:25 To: Weems, Joyce; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature And where would one go to see this proposal? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 12:39 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Physician signature It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 7 Date: 22 Dec 2010 14:48:21 -0500 From: Alisha Dynan Subject: [Histonet] High Paying Job Opportunity - Live anywhere To: histonet@lists.utsouthwestern.edu Message-ID: <1222998903.1293047301530.JavaMail.cfservice@sl4app2> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am a healthcare recruiter, specifically focused on the laboratory/pathology space. I have openings all across the country and would be interested in speaking with anyone interested in searching for a new job. I am currently working on an amazing opportunity with a cancer diagnostics company. This company is looking to hire on Technical Support Specialists in several areas across the country. They currently are looking for people located anywhere West of the Mississippi River or in Atlanta, GA or in Nashville, TN or in New York City or in Philadelphia. The TSS would be responsible for training pathologists, surgeons, radiologists, and pathologist assistant's to ensure adequate sampling on breast cancer and colon cancer tissues. They are looking for someone with a Bachelor's Degree and 5+ years experience in tissue grossing/sampling, histology, or in a pathology or surgical capacity. The ideal candidate with be PA(ASCP) or HTL(ASCP) certified. This position requires extensive travel, paid for by the company. The compensation package is fantastic with a high base salary and up to a 20% bonus. If interested in learning more details, please email me at alisha@ka-recruiting.com. If you are not interested but know of someone you could recommend for this position, please pass on their contact information to me. We have a referral bonus for anyone you refer to me that I place into a new job. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 8 Date: Wed, 22 Dec 2010 20:23:54 +0000 From: Martha Ward Subject: [Histonet] Fisher MicroProbe manual staining system To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have one of these systems that they would be willing to donate? One of our pathologists is going on a mission trip to Honduras and would like to take one of these down there with him. We used to have the set up but recently got rid of it. (Timing is everything!!) Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ------------------------------ Message: 9 Date: Wed, 22 Dec 2010 12:33:14 -0800 From: "Morken, Tim" Subject: RE: [Histonet] RE: Physician signature To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Joyce wrote: "The Federal Register is always a good place to find interesting reading" Gee, Joyce, all these years I've known you and I never realized you are a masochist!! But maybe the fact you are the go-to person for regulations should have been a clue!;) Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 11:48 AM To: Mike Pence; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature The Federal Register is always a good place to find interesting reading. http://edocket.access.gpo.gov/2010/pdf/2010-15900.pdf Search for signature and you can find it...starts on page 40161. In the middle column on page 40162 charges for TC for AP are mentioned. consistent with the principle in ? 410.32(a) that a written order for diagnostic tests including those paid under the CLFS and those that are not paid under the CLFS (for example, that are paid under the PFS or under the OPPS), such as X-rays, MRIs, and the TC of physician pathology services, must be signed by the ordering physician or NPP. That is, the policy that signatures are not required on requisitions for clinical diagnostic laboratory tests paid based on the CLFS applies only to requisitions (as opposed to written orders) (74 FR 33642). Blah blah blah.... At the end they say "We believe that this policy would result in a less confusing process." Merry Christmas, Everyone! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, December 22, 2010 14:25 To: Weems, Joyce; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature And where would one go to see this proposal? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 12:39 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Physician signature It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 22 Dec 2010 15:36:39 -0500 From: John Shelley Subject: [Histonet] Frozen Specimen Handling To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi HistoLand, I have a question regarding frozen specimen handling. I have a group that gave me hearts, brown fat and white fat and there are some issues with the morphology of the tissue and also the cutting quality. Unfortunately I was part of the whole process from tissue harvesting to cutting and staining of specimens. We placed harvested tissue in a cryo mold with OCT and snap froze the block in LN2 and then placed on dry ice. I know sometimes the quick freeze can cause some effects but was wondering how others are addressing the freezing of tissue, especially fat. We have frozen fat this way before and did not have the same issues. Any help or suggestions would be great. Thanks!!! Kind Regards! John Shelley ------------------------------ Message: 11 Date: Wed, 22 Dec 2010 15:43:19 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] RE: Physician signature To: "Morken, Tim" , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332B98@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" Oh dear, now you all know! You all ought to see where our tax dollars are going!! Such a convoluted mess. I don't know how the writer can understand what he/she is saying! We could save so may trees if they would just get to the point! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Wednesday, December 22, 2010 15:33 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature Joyce wrote: "The Federal Register is always a good place to find interesting reading" Gee, Joyce, all these years I've known you and I never realized you are a masochist!! But maybe the fact you are the go-to person for regulations should have been a clue!;) Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 11:48 AM To: Mike Pence; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature The Federal Register is always a good place to find interesting reading. http://edocket.access.gpo.gov/2010/pdf/2010-15900.pdf Search for signature and you can find it...starts on page 40161. In the middle column on page 40162 charges for TC for AP are mentioned. consistent with the principle in ? 410.32(a) that a written order for diagnostic tests including those paid under the CLFS and those that are not paid under the CLFS (for example, that are paid under the PFS or under the OPPS), such as X-rays, MRIs, and the TC of physician pathology services, must be signed by the ordering physician or NPP. That is, the policy that signatures are not required on requisitions for clinical diagnostic laboratory tests paid based on the CLFS applies only to requisitions (as opposed to written orders) (74 FR 33642). Blah blah blah.... At the end they say "We believe that this policy would result in a less confusing process." Merry Christmas, Everyone! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, December 22, 2010 14:25 To: Weems, Joyce; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Physician signature And where would one go to see this proposal? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 22, 2010 12:39 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Physician signature It specifically mentions AP technical charges in the proposal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Physician signature According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 12 Date: Wed, 22 Dec 2010 16:08:20 -0500 From: Clare Thornton Subject: [Histonet] Symphony cover slipper To: "'Histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Anyone else out there have issues with misaligned cover slips on the Symphony stainer? We have two stainers and there doesn't seem to be a pattern. Sometimes it's one stainer, sometimes the other, sometimes every tray, sometimes one out of ten trays. Usually the cover slip is off just enough to have a sharp edge and we need to fix it, which for any other Symphony owners you know that's a pain. Whenever they adjust the cover slipper, it will work well for a day or two then start acting up again. Is it just us? Thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ------------------------------ Message: 13 Date: Wed, 22 Dec 2010 16:09:00 -0500 From: Drew Meyer <41dmb41@gmail.com> Subject: [Histonet] VIP 1000 Help To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We've got the old workhorse, the VIP 1000, that we still use. During a move, the guide, which shows all the commands you can do with that magnet, got lost. Is there anyone out there that has this and could send or fax me a copy? It would be much appreciated! Thanks, Drew Meyer -- ~Love is like a booger. You keep picking at it until you get it, then wonder what to do with it. ------------------------------ Message: 14 Date: Wed, 22 Dec 2010 15:18:18 -0700 From: "Liz Chlipala" Subject: [Histonet] FW: ASCP Action Alert - Physician Signature Requirement _FYI To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" FYI - saw alot about this today, just got this e-mail from ASCP Liz -----Original Message----- From: American Society for Clinical Pathology [mailto:ascp@site1.ascpmail.org] Sent: Wed 12/22/2010 2:42 PM To: Liz Chlipala Subject: ASCP Action Alert - Physician Signature Requirement Physician Signature Requirement Burdens Labs, Jeopardizes Patients ASCP urges you to act before the Jan. 3 deadline Recently, the Centers for Medicare and Medicaid Services (CMS) finalized its Medicare Physician Fee Schedule final rule for the 2011 calendar year. The rule includes a troubling policy change requiring a physician's or qualified nonphysician practitioner's (NPP) signature on requisitions for clinical diagnostic laboratory tests paid under the clinical laboratory fee schedule (effective Jan. 1, 2011). CMS defines a requisition as "the actual paperwork, such as a form, which is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient." Currently, a physician signature is required only on orders for laboratory services. ASCP believes the new rule could adversely affect patient care and complicate the provision of the laboratory services. In cases where a signature from the ordering physician or NPP is absent, laboratories could be left scrambling trying to obtain the signature. Late Breaking News Rule: Recently CMS has partially agreed to a delayed implementation request from ASCP and other members of the clinical laboratory coalition organizations. CMS will delay implementation of the rule for three months. During this period, ASCP will continue to pressure CMS to withdraw its new proposal. ASCP urges you to contact CMS and tell them of your opposition to the policy change. Contact CMS before the Jan. 3 Comment Deadline to maximize the effectiveness of your voice. Read more and take action today . . . Tell a friend: Not everyone receives ASCP's Action Alerts, so please forward this message to your peers and co-workers! 2010 American Society for Clinical Pathology 33 West Monroe Street, Suite 1600, Chicago, IL 60603 312.541.4999 ~ info@ascp.org ------------------------------ Message: 15 Date: Wed, 22 Dec 2010 16:52:13 -0600 From: Subject: RE: [Histonet] Frozen Specimen Handling To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Cutting frozen fat is virtually impossible. There are a few articles I have seen online (one is called something along the lines of "the fat demon" or something pun-tastic like that). Because fat is so oily it usually doesn't freeze at all. If you have a lot of fat in the tissue you are trying to freeze and not just a big hunk of fat (say an inguinal LN incased in fat), cutting the fatty tissue can be a challenge too. I have heard you can try and cut it thicker, but some doc's don't like that. Another thing we tried was fixing the fat for about 15 minutes in Pen-Fix (or some other alcoholic formalin mixture), if you have this much time then that can also help. Good Luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, December 22, 2010 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Specimen Handling Hi HistoLand, I have a question regarding frozen specimen handling. I have a group that gave me hearts, brown fat and white fat and there are some issues with the morphology of the tissue and also the cutting quality. Unfortunately I was part of the whole process from tissue harvesting to cutting and staining of specimens. We placed harvested tissue in a cryo mold with OCT and snap froze the block in LN2 and then placed on dry ice. I know sometimes the quick freeze can cause some effects but was wondering how others are addressing the freezing of tissue, especially fat. We have frozen fat this way before and did not have the same issues. Any help or suggestions would be great. Thanks!!! Kind Regards! John Shelley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 22 Dec 2010 18:06:19 -0500 (EST) From: alineumann@aol.com Subject: [Histonet] Used embedding center and microtome To: histonet@lists.utsouthwestern.edu Message-ID: <8CD7043FA4CB24F-478-1572A@Webmail-d112.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello Histonetters, especially those in the Denver area, we have the following available: ? A functional?embedding center, Reichert Histostat 8034, purchased in 1/2002,?used,?for $1500, with manual.? Will sell for $700, or $1100 if shipping is required (ie, if you cannot pick it up from our Denver suburb location).. ? A functional microtome, Leitz 1512,??purchased used in 10/2004 for $1500.??We can sell for $700, or $850 if shipping is required.? Pay Pal and corporate checks accepted.? Please contact me at the email or phone below if interested.? Thank you, Alice Neumann MD Western Wyoming Pathology PC Pinnacle Pathology PC 9423 West Kentucky Place Lakewood, CO?? 80226 24 Hour Cell Phone:? 307-413-4092 alineumann@aol.com? ? ? ------------------------------ Message: 17 Date: Wed, 22 Dec 2010 15:16:37 -0800 From: Victor Tobias Subject: [Histonet] CAP Question regarding procedure manual To: Histonet Message-ID: <4D1286D5.9030207@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Is there a requirement to have a signature of every staff member on a procedure if they perform that procedure in a manual? Wouldn't one signature on a cover page suffice that you have read and understand what is in the manual? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ------------------------------ Message: 18 Date: Wed, 22 Dec 2010 18:34:54 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] CAP Question regarding procedure manual To: Victor Tobias , Histonet Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164044B332BCB@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Please tell me that is good enough! We do that here - for each manual. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, December 22, 2010 18:17 To: Histonet Subject: [Histonet] CAP Question regarding procedure manual Is there a requirement to have a signature of every staff member on a procedure if they perform that procedure in a manual? Wouldn't one signature on a cover page suffice that you have read and understand what is in the manual? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 19 Date: Wed, 22 Dec 2010 16:46:09 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] CAP Question regarding procedure manual To: , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" You will need a signature for the manual and for each procedure that is implemented or amended during your cycle year. If your manual is very stable, a yearly signature page for the entire manual may suffice, but the inspector will note the date everyone signed the manual review page and then go through the manual to see if there are any implementation dates or procedure change dates after the manual review date. Each occurrence found one can be a deficiency. In my lab, it is not uncommon for new procedures to be written and implemented or existing procedures implemented during a cycle year and all these events require a signature by System Medical Director, Site Medical Director, Site Lab Director, System Technical Specialist (responsible for physically writing) and all employees affected. You must prove to CAP that all personnel know where the procedure manual is located, that they have reviewed the manual as a whole and that they have reviewed all additions implemented. We have employees sign each technical alert, procedure update/change and new procedures, along with a once a year review of all manuals. This type of signature trail is part of and right in line with quality management and document control. This process alone can be a daunting task and requires a lot or forethought. To better manage this process, we are moving to an electronic on-line, web based procedure manual with document control functions that tracks usage by employee and captures electronic signatures for each step of the creation, review and implementation process. Thankfully, this process is the responsibility of our Technical Specialist for Anatomic Pathology and our System Quality Department. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 22 Dec 2010 15:16:37 -0800 > From: victor@pathology.washington.edu > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CAP Question regarding procedure manual > > Is there a requirement to have a signature of every staff member on a > procedure if they perform that procedure in a manual? Wouldn't one > signature on a cover page suffice that you have read and understand what > is in the manual? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 22 Dec 2010 16:57:52 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] CAP Question regarding procedure manual To: "WILLIAM DESALVO" , , "histonet" Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C6AEA@EXCHANGECLUSTER.yumaregional.local> Content-Type: text/plain; charset="us-ascii" Currently Victor we use to have the techs sign off on all the policies and procedures. As Bill stated this is a big pain. We are now all electonic and have gone live with I-Passport our document control software,, this has saved tons of time and effort. Electronic is the way to go especially for updates and new information. Users are notifed by email and it allows the supervisor to keep track of edits and much more. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, December 22, 2010 4:46 PM To: victor@pathology.washington.edu; histonet Subject: RE: [Histonet] CAP Question regarding procedure manual You will need a signature for the manual and for each procedure that is implemented or amended during your cycle year. If your manual is very stable, a yearly signature page for the entire manual may suffice, but the inspector will note the date everyone signed the manual review page and then go through the manual to see if there are any implementation dates or procedure change dates after the manual review date. Each occurrence found one can be a deficiency. In my lab, it is not uncommon for new procedures to be written and implemented or existing procedures implemented during a cycle year and all these events require a signature by System Medical Director, Site Medical Director, Site Lab Director, System Technical Specialist (responsible for physically writing) and all employees affected. You must prove to CAP that all personnel know where the procedure manual is located, that they have reviewed the manual as a whole and that they have reviewed all additions implemented. We have employees sign each technical alert, procedure update/change and new procedures, along with a once a year review of all manuals. This type of signature trail is part of and right in line with quality management and document control. This process alone can be a daunting task and requires a lot or forethought. To better manage this process, we are moving to an electronic on-line, web based procedure manual with document control functions that tracks usage by employee and captures electronic signatures for each step of the creation, review and implementation process. Thankfully, this process is the responsibility of our Technical Specialist for Anatomic Pathology and our System Quality Department. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 22 Dec 2010 15:16:37 -0800 > From: victor@pathology.washington.edu > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CAP Question regarding procedure manual > > Is there a requirement to have a signature of every staff member on a > procedure if they perform that procedure in a manual? Wouldn't one > signature on a cover page suffice that you have read and understand > what is in the manual? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ ------------------------------ Message: 21 Date: Thu, 23 Dec 2010 07:53:22 -0500 From: BSullivan@shorememorial.org Subject: Re: [Histonet] CAP Question regarding procedure manual To: Victor Tobias Cc: Histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Victor, To my knowledge all you need is proof that the staff reviewed the manuals. We accomplish this by a sign off sheet in the front of each manual we use. The Supervisor, or designee, needs to review and sign off on each procedure in each manual. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Victor Tobias To Sent by: Histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] CAP Question regarding 12/22/2010 06:16 procedure manual PM Is there a requirement to have a signature of every staff member on a procedure if they perform that procedure in a manual? Wouldn't one signature on a cover page suffice that you have read and understand what is in the manual? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 85, Issue 21 **************************************** This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From mw <@t> personifysearch.com Fri Jan 7 15:24:40 2011 From: mw <@t> personifysearch.com (Matt Ward) Date: Fri Jan 7 15:24:45 2011 Subject: [Histonet] Histology Training Specialist - Great Opportunity Message-ID: <23b867f637531815fb478a88cfed78a0@mail.gmail.com> Happy New Year Histonet! Our team at Personify is searching for a histology professional for a Histology Training position with a world leader based out of the Chicago area. Please contact me directly to learn more. Thanks! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com *Histology Training Specialist * *The Company:* Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *The Opportunity:* The company currently has an opening for a Histology Training Specialist. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: Depends on Experience Other: Full benefits - 401k program/matching *Primary Responsibilities:* The primary responsibility of this role will be to provide technical phone support by answering questions, troubleshooting problems, logging and closing complaint files and escalating major issues to appropriate company personnel. This role will also provide technical training on specified products in the company's newly constructed state-of-the-art Customer Support Laboratory. Training programs are designed for small groups to ensure maximum customer learning and satisfaction. Additional Responsibilities: - Provide product and applications phone support to end-users, field personnel and dealers for all product lines - Log all calls into Customer Support Database - Participate in development of training materials and conduct classes and labs for customers, employees and others as needed - Serve as technical liaison to Customer Service/Field Service/Product Management departments *Education and Experience Required:* Ability to interact with various people in a calm and positive fashion and the ability to effectively communicate information to groups of participants is required. Experience with data entry, MS Office programs (PowerPoint, Lotus Notes, Word) is also required. HT/HTL/QIHC (ASCP) is helpful but not required. Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From mhanna <@t> histosearch.com Fri Jan 7 16:42:28 2011 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Fri Jan 7 16:42:35 2011 Subject: [Histonet] Charged slides In-Reply-To: <3201CF51728F6048A24FA3AFFFEEF1D31785B5BE0D@JHEMTEXVS3.win.ad.jhu.edu> References: <3201CF51728F6048A24FA3AFFFEEF1D31785B5BE0D@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: Hi Helen, Plus is the branding for silanized slides, so they are the same. As far as magical, I'm not sure, but the silanization procedure covalently bonds the amino groups in APES (3- Aminopropyltriethoxysilane, available from Sigma) to the silicate ions on the surface of the glass, creating a positive charge on the slide. Polylysine coating also puts a positive charge on the slide, but it is just a coating, unlike the chemically reacted covalent bond of the silanization procedure. A positive charge on the surface of the slide allows for strong bonding with negatively charged formalin fixed tissue. John Kiernan wrote a paper many years back on adhesives for slides that shows the procedure and chemical reaction in silanizing slides. It is available here. http://publish.uwo.ca/~jkiernan/adhesivs.htm Kind Regards, Marvin Hanna On Jan 6, 2011, at 5:43 PM, Helen Fedor wrote: > Hi, A question has come up regarding the different methods used to > put a charge on the slide. We recently ordered some plus slides and > the boxes they are packed in say silanized slides, but they say > "plus" on the slide . We don't want to use these for our clients if > they are not going to be getting the same results as the former > "plus" slides that we were using. I was under the delusion that > Plus slides somehow are magically charged without any coating > process taking place. > > So does anyone know exactly how a "plus" slide gets its charge? Do > they all get dipped in APES, or Polylysine? > > Thanks for your help. > > -- > Helen > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotech <@t> imagesbyhopper.com Sat Jan 8 15:32:50 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Jan 8 15:33:52 2011 Subject: [Histonet] Lost Blocks In-Reply-To: References: Message-ID: Our method isn't as technology savvy, but it works well for us. All blocks submitted for processing are written down on our "piece sheet". At the end of the grossing day, two staff members compare the blocks in the processing basket to those listed on the piece sheet. At embedding, the histotech checks off the blocks prior to embedding. On Jan 7, 2011, at 3:25 PM, Patrick Laurie wrote: > Here we take a picture of the blocks before they go on the processor. We > have a barcoding system that lets me know who embedded what and when. With > over 140,000 blocks/year we only have had one instance where a block was > lost between grossing and embedding. Because we have some off-site > pathologists and grossing staff, our lab relies very heavily on tracking > every specimen that comes in, every block that goes to histology and every > stained slide that goes to our pathologists. > > Good luck, it can be frustrating when you lose a block. > > On Fri, Jan 7, 2011 at 9:45 AM, Paula Wilder wrote: > >> >> HI all, >> >> Does anyone have a procedure outlining checks and balances to assure blocks >> have been placed on the processor, blocks that are missing, either before or >> after embedding, etc. Any help would be greatly appreciated! >> Thank you, >> >> Paula Wilder >> St. Joseph Medical Center >> Towson, MD 21204 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology & Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > plaurie@cellnetix.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From irena.kirbis <@t> hotmail.com Sun Jan 9 00:58:23 2011 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Sun Jan 9 00:58:28 2011 Subject: [Histonet] ArrayMold System In-Reply-To: <130E8991F210424096EFC6F42EA33B24071EC131@LSCOEXCH1.lsmaster.lifespan.org> References: <130E8991F210424096EFC6F42EA33B24071EC131@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: so far we have good experiencies with it, it's very easy to use eg. it's easy to prepare and construct TM, however we still struggle how to handle prepared TM in order to keep all punches on the cut sections, it seems that this part request some additional adjustment - at what temperature and how many cycles are needed to assure stabile TM, and of course it seems that cutting is crucial too. last but not least it's much much cheaper than Beecher, so for the labs just introducing and trying out TM in practice this way is better Irena > From: NHeath@Lifespan.org > To: histonet@lists.utsouthwestern.edu > CC: histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] ArrayMold System > > Wondering if anyone has used/purchased the ArrayMold "tissue micro array > system"?? Positive and negative feedback would be greatly appreciated :) > > Nancy Heath, HT(ASCP) > > March 10, 2011 is Histotechnology Professionals Day > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlab1 <@t> hotmail.com Sun Jan 9 07:52:28 2011 From: pathlab1 <@t> hotmail.com (Matthew Fleming) Date: Sun Jan 9 07:52:32 2011 Subject: [Histonet] Histotech position in Milwaukee, Wisconsin Message-ID: Histotech needed for full or part-time position with small pathology laboratory in Milwaukee, WI. We offer a competitive salary, commensurate with experience. There are also excellent benefits, flexible hours, and congenial coworkers. If interested, please send inquiry to pathlab1@hotmail.com. Please send your CV or a description of your educational background and work experience. Please also indicate your salary requirements, whether you are interested in full or part-time work, and the hours you would be available. Please also provide contact information. References are not necessary for your initial inquiry but will be requested later. From Barbara.Crill <@t> LPNT.net Mon Jan 10 07:11:49 2011 From: Barbara.Crill <@t> LPNT.net (Barbara.Crill@LPNT.net) Date: Mon Jan 10 07:11:59 2011 Subject: [Histonet] fetal demise In-Reply-To: <201101081809.p08I9mOU031731@NADCLZMSGPMG01E.medcity.net> References: <201101081809.p08I9mOU031731@NADCLZMSGPMG01E.medcity.net> Message-ID: <7DA79EBDBD92BF408EF392413737878D392C24548D@NADCWPMSGCMS01.hca.corpad.net> I am searching for a regulation referencing proper disposal of the fetus. Does anyone know is there a written policy? ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 From kc <@t> ka-recruiting.com Mon Jan 10 08:40:49 2011 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Mon Jan 10 08:40:25 2011 Subject: [Histonet] Histology Manager Job in NYC Message-ID: <468226480.1294670449613.JavaMail.cfservice@SL4APP4> I am a one of the founders of a healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in a career change in 2011. My services are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Laboratory Manager opening at a Dermpath Lab. My client is a Pre-IPO Pathology company who is quickly establishing itself as an industry leader. I'm contacting you to see if you'd be interested in pursuing the Lab Manager position they have open in their Dermpath Lab in Long Island, NY. This state of the art lab s looking for someone with prior management experience to run and manage a lab consisting of 10 FTE's. This Manager will be responsible for overseeing the day to day operations of the lab while being able to handle bench work as needed. My clients offers one of the best compensation & benefits package around including relocation assistance when necessary. For consideration you must have a Bachelor's Degree, be HT or HTL (ASCP) certified and licensed in the State of NY. A minimum of 2 years of prior supervisory or management experience is preferred. If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com Please contact me if you're interested in learning more about this position or in exploring some of the other numerous job openings we have available across the country. I wish you all the best in 2011! Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From historsd <@t> aol.com Mon Jan 10 10:02:03 2011 From: historsd <@t> aol.com (historsd@aol.com) Date: Mon Jan 10 10:02:09 2011 Subject: [Histonet] Mohs tech needed Message-ID: <8CD7EF6DAEED407-1EE8-217C0@webmail-d081.sysops.aol.com> PT Histotech position available in Mohs dermatology office in Jacksonville, Florida. Position availability starting immediately for a few hours per day, a few days per week, changing to a daily PT or FT position from June - August to cover a maternity leave, and PT/PRN therafter. Must be a detail oriented, fast paced multi - tasker. Frozen section experience preferred. Basic computer knowledge and efficient typing skills also a plus. Please forward resumes to med9@bellsouth.net. From dellav <@t> musc.edu Mon Jan 10 10:23:56 2011 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Jan 10 10:24:09 2011 Subject: [Histonet] RE: Feulgen Stain for DNA In-Reply-To: References: Message-ID: Feulgen demonstrates the deoxyribose which is in the backbone of each helical strand. It shouldn't matter if single or double stranded. Hydrolysis is the key step and can be overdone. Textbooks list using hot 1N HCl Many years ago Jules Elias and I co-authored a paper where we demonstrated that the use of 5N HCl at room temperature provided more consistent results Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 Learn more about Histotechnology Professionals Day at www.nsh.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, January 07, 2011 4:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Feulgen Stain for DNA Hello Everyone I have tried to find this answer on the web and in references but I need some help. With respects to the Feulgen stain, how sensitive is it? Can it detect smaller fragments of DNA? Does it have to be double stranded or will single stranded stain as well? Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Mon Jan 10 11:04:19 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Mon Jan 10 11:04:22 2011 Subject: [Histonet] gloves Message-ID: <000601cbb0e8$6bb5aa00$4320fe00$@com> Happy Monday Histonetters I am looking for a reasonably priced, non-latex glove that will hold up to xylene. The purple knight from Kimberly-Clark works great but is cost prohibited. My general manager want me to find a cheaper alternative. I have had several samples but none will hold up to any exposure to xylene. We were previously using the black panther gloves which were working well, but according to our supplier are no longer available. Any advice from you vast experience would be appreciated. Thanks in advance. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com From Nalini.Makhijani <@t> va.gov Mon Jan 10 11:31:51 2011 From: Nalini.Makhijani <@t> va.gov (Makhijani, Nalini S) Date: Mon Jan 10 11:32:39 2011 Subject: [Histonet] Klotho Message-ID: <715FA772CEA81A47B1A762AB6E45123A0882E831@VHAV22MSGA3.v22.med.va.gov> Has anyone tried detecting Klotho for cell aging via IHC or Western or ELISA? Is the antibody from Santa Cruz Biotechnology any good or is there any other preference? Thanks, Nalini From Ken_Marissael <@t> vwr.com Mon Jan 10 13:04:07 2011 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Mon Jan 10 13:04:22 2011 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 01/10/2011 and will not return until 01/14/2011. I will be away at the VWR National Sales Meeting and will have very limited access to phone and e-mail> While I am out, please contact VWR Customer Service at 800-932-5000. From Marilyn.A.Weiss <@t> kp.org Mon Jan 10 18:01:53 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Mon Jan 10 18:02:08 2011 Subject: [Histonet] I Message-ID: I will be out of the office starting 01/10/2011 and will not return until 01/31/2011. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. From kdwyer3322 <@t> aol.com Tue Jan 11 07:49:54 2011 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Tue Jan 11 07:50:22 2011 Subject: [Histonet] Texas Society for Histotechnology 2001 State Meeting Message-ID: <8CD7FAD8EFAAECA-708-C2F6@webmail-d040.sysops.aol.com> All, The Texas Society for Histotechnology 2011 meeting will be held March 31 to April 3, 2011 at the Marriott Dallas Legacy Hotel in Plano, Texas. The program is complete and will be shortly available on our website at txsh.org however if you would like an electonic copy please contact: Kdwyer3322@aol.com OR Veronida@baylorhealth.edu Regards, TSH convention committee From kdwyer3322 <@t> aol.com Tue Jan 11 07:53:01 2011 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Tue Jan 11 07:53:17 2011 Subject: [Histonet] Texas Society for Histotechnology 2011 State Meeting Message-ID: <8CD7FADFF22E728-708-C3C8@webmail-d040.sysops.aol.com> All, The Texas Society for Histotechnology 2011 meeting will be held March 31 to April 3, 2011 at the Marriott Dallas Legacy Hotel in Plano, Texas. The program is complete and will be shortly available on our website at txsh.org however if you would like an electonic copy please contact: Kdwyer3322@aol.com OR Veronida@baylorhealth.edu Regards, TSH convention committee From Janice.Mahoney <@t> alegent.org Tue Jan 11 09:01:47 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Jan 11 09:02:24 2011 Subject: [Histonet] RE: fetal demise In-Reply-To: <7DA79EBDBD92BF408EF392413737878D392C24548D@NADCWPMSGCMS01.hca.corpad.net> References: <201101081809.p08I9mOU031731@NADCLZMSGPMG01E.medcity.net> <7DA79EBDBD92BF408EF392413737878D392C24548D@NADCWPMSGCMS01.hca.corpad.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B535500E@EXCHMBC2.ad.ah.local> I'd suggest you check with your local coroner, or state statutes regarding fetal demise. There are laws that govern at what gestational age a fetus can be considered a surgical specimen and when it must go to a funeral home. If you do not already have policies check with labor and delivery or your institutions legal department. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Monday, January 10, 2011 7:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fetal demise I am searching for a regulation referencing proper disposal of the fetus. Does anyone know is there a written policy? ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Kimberly.Blundon <@t> drdc-rddc.gc.ca Tue Jan 11 09:28:42 2011 From: Kimberly.Blundon <@t> drdc-rddc.gc.ca (Blundon, Kimberly) Date: Tue Jan 11 09:28:47 2011 Subject: [Histonet] Cryostat help Message-ID: <42DFE1A029181B4B8CCBA7261B52D7656B0E53@suffieldex01.suffield.drdc-rddc.gc.ca> Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would come to the experts. Thanks for your help! Kimberly From rjbuesa <@t> yahoo.com Tue Jan 11 10:28:57 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 11 10:29:01 2011 Subject: [Histonet] Cryostat help In-Reply-To: <42DFE1A029181B4B8CCBA7261B52D7656B0E53@suffieldex01.suffield.drdc-rddc.gc.ca> Message-ID: <997671.57201.qm@web65705.mail.ac4.yahoo.com> That depends on what staining?you?need to use. If an IHC, just fix the sections in acetone, air dry and proceed without HIER If H&E fix quickly with formalin 10%, wash ? hematoxylin for 30 secs without differentiation ? eosin 15 sec? dehydrate and mount. Other HC procedures all will require fixation and using the specific protocol. Always remember that it is a frozen section and therefore more delicate and less adhered than a paraffin section. Ren? J. --- On Tue, 1/11/11, Blundon, Kimberly wrote: From: Blundon, Kimberly Subject: [Histonet] Cryostat help To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 11, 2011, 10:28 AM Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would come to the experts. Thanks for your help! Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Tue Jan 11 11:00:56 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Tue Jan 11 11:01:02 2011 Subject: [Histonet] Cryostat help In-Reply-To: <997671.57201.qm@web65705.mail.ac4.yahoo.com> References: <42DFE1A029181B4B8CCBA7261B52D7656B0E53@suffieldex01.suffield.drdc-rddc.gc.ca> <997671.57201.qm@web65705.mail.ac4.yahoo.com> Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7682C5@CMHEXCC01MBX.childrensmemorial.org> We have a similar frozen section protocol but we fix the slide in 95% alcohol for ~1minute and then stain with Gill's Hematoxylin for 1 minute, rinse, blue and then alcohol, foloowed by Eosin for ~ 15secs.- dehydrate and mount. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 11, 2011 10:29 AM To: histonet@lists.utsouthwestern.edu; KimberlyBlundon Subject: Re: [Histonet] Cryostat help That depends on what staining?you?need to use. If an IHC, just fix the sections in acetone, air dry and proceed without HIER If H&E fix quickly with formalin 10%, wash ? hematoxylin for 30 secs without differentiation ? eosin 15 sec? dehydrate and mount. Other HC procedures all will require fixation and using the specific protocol. Always remember that it is a frozen section and therefore more delicate and less adhered than a paraffin section. Ren? J. --- On Tue, 1/11/11, Blundon, Kimberly wrote: From: Blundon, Kimberly Subject: [Histonet] Cryostat help To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 11, 2011, 10:28 AM Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would come to the experts. Thanks for your help! Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtaylor <@t> meriter.com Tue Jan 11 11:21:30 2011 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Tue Jan 11 11:21:34 2011 Subject: [Histonet] batching controls Message-ID: Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From laurie.colbert <@t> huntingtonhospital.com Tue Jan 11 11:39:29 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Jan 11 11:39:32 2011 Subject: [Histonet] batching controls In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> We batch our controls. We still try to put the control tissue on a patient slide, and then we reference that case that has the control on the other cases that don't have a control. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 9:22 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Jan 11 11:42:49 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jan 11 11:42:52 2011 Subject: [Histonet] batching controls In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E273839A176@UWHC-MAIL01.uwhis.hosp.wisc.edu> Hi Jean, We run one positive control for any given antibody that is ordered on multiple cases and all are run at the same time on the same instrument. Whenever possible that control has the patient tissue of one of the cases on it. That case is referenced for the pathologists reading the other cases with that antibody so they can find the positive control if they feel the need. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 11:22 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Jan 11 11:44:02 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 11 11:45:01 2011 Subject: [Histonet] batching controls In-Reply-To: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> Message-ID: How does this work if you need to send the case out for a consult and the control is on another patient's slide? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Tuesday, January 11, 2011 12:39 PM To: Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We batch our controls. We still try to put the control tissue on a patient slide, and then we reference that case that has the control on the other cases that don't have a control. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 9:22 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From ancillarypath <@t> mac.com Tue Jan 11 11:57:15 2011 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Tue Jan 11 11:57:21 2011 Subject: [IHCRG] RE: [Histonet] batching controls In-Reply-To: <8C023B4AB999614BA4791BAEB26E273839A176@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <8C023B4AB999614BA4791BAEB26E273839A176@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <9EFD9EE5-92FA-4705-B5BB-2B5860A45D55@mac.com> I agree with Linda. One positive control per *batch*, not per case. The positive control tissue can be placed on one of the patient's specimens, which saves you money on the detection system and antibody as well. We should be careful also about treating different drawers with different runs (such as the Bond, for example) as a separate batch, which requires its own set of controls. Some antibodies that have internal controls on every patient tissue do not require external controls, such as vimentin, CD31, CD34, etc. Predictive markers positive controls should always be the same controls, and can only be changed after verifying that the new control is okay to run and give you the desired signal. Thanks, Hadi Yaziji ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories 7480 SW 40 St, Suite 700 Miami, FL 33155 Tel 305-267-7979 F. 786-513-0175 www.vitromolecular.com On Jan 11, 2011, at 12:42 PM, Sebree Linda A wrote: > Hi Jean, > > We run one positive control for any given antibody that is ordered on > multiple cases and all are run at the same time on the same instrument. > Whenever possible that control has the patient tissue of one of the > cases on it. That case is referenced for the pathologists reading the > other cases with that antibody so they can find the positive control if > they feel the need. > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, > Jean > Sent: Tuesday, January 11, 2011 11:22 AM > To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] batching controls > > Hi Everyone, > > I'd like to know how many labs batch their controls when the same > antibody is ordered on multiple cases. Do you run the control on a > separate slide, or with one of the cases ordered? > > Thank you! > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From joseph-galbraith <@t> uiowa.edu Tue Jan 11 12:03:56 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Tue Jan 11 12:04:32 2011 Subject: [Histonet] batching controls In-Reply-To: References: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> Message-ID: <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 11, 2011 11:44 AM To: Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls How does this work if you need to send the case out for a consult and the control is on another patient's slide? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Tuesday, January 11, 2011 12:39 PM To: Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We batch our controls. We still try to put the control tissue on a patient slide, and then we reference that case that has the control on the other cases that don't have a control. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 9:22 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From ancillarypath <@t> mac.com Tue Jan 11 12:08:54 2011 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Tue Jan 11 12:09:16 2011 Subject: [IHCRG] RE: [Histonet] batching controls In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> References: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: This is the most cautious approach, and for antibodies where there is no internal controls that would be your only way to verify that the studies worked. But it's also associated with some unnecessary rejection of good studies because controls aren't treated the same way as the patient slides. Good approach and we do it on our "technical-only" cases where the slides are returned to the outside pathologists for interpretation. Hadi On Jan 11, 2011, at 1:03 PM, Galbraith, Joe wrote: > We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. > > Joe > joseph-galbraith@uiowa.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni > Sent: Tuesday, January 11, 2011 11:44 AM > To: Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] batching controls > > How does this work if you need to send the case out for a consult and the control is on another patient's slide? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie > Colbert > Sent: Tuesday, January 11, 2011 12:39 PM > To: Taylor, Jean; ihcrg@googlegroups.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] batching controls > > > We batch our controls. We still try to put the control tissue on a > patient slide, and then we reference that case that has the control on > the other cases that don't have a control. > > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, > Jean > Sent: Tuesday, January 11, 2011 9:22 AM > To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] batching controls > > Hi Everyone, > > I'd like to know how many labs batch their controls when the same > antibody is ordered on multiple cases. Do you run the control on a > separate slide, or with one of the cases ordered? > > Thank you! > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From LSetlak <@t> childrensmemorial.org Tue Jan 11 12:12:02 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Tue Jan 11 12:12:07 2011 Subject: [Histonet] RE: batching controls In-Reply-To: References: Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7682C6@CMHEXCC01MBX.childrensmemorial.org> Hi, We will use one control if the different cases are going to the same pathologist. It varies - sometimes we run a separate slide, other time it's with the tissue from one of the cases. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 11:22 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlschneider <@t> gmail.com Tue Jan 11 12:13:25 2011 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Tue Jan 11 12:13:28 2011 Subject: [Histonet] Job Opportunity in West Texas: Histology Supervisor/Manager Message-ID: We're a pathology group in West Texas, and we're seeking a knowledgeable histotechnologist, with management skills, to supervise our histology lab. Experience in immunohistochemistry is essential. If you're interested, drop me an email. Thank you, Daniel Schneider, MD From cmiller <@t> physlab.com Tue Jan 11 12:16:25 2011 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Jan 11 12:16:35 2011 Subject: [Histonet] batching controls In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> References: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: We run 1 control per antibody within a case, so if we had 3 mart-1 on 3 separate cases I would run a control for each case Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor, Safety Officer, Physicians Laboratory Services Omaha, NE. 68127 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Tuesday, January 11, 2011 12:04 PM To: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 11, 2011 11:44 AM To: Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls How does this work if you need to send the case out for a consult and the control is on another patient's slide? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Tuesday, January 11, 2011 12:39 PM To: Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We batch our controls. We still try to put the control tissue on a patient slide, and then we reference that case that has the control on the other cases that don't have a control. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 9:22 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From higginst <@t> amapath.com Tue Jan 11 12:09:54 2011 From: higginst <@t> amapath.com (Tim Higgins) Date: Tue Jan 11 12:16:44 2011 Subject: [IHCRG] RE: [Histonet] batching controls In-Reply-To: <9EFD9EE5-92FA-4705-B5BB-2B5860A45D55@mac.com> References: <8C023B4AB999614BA4791BAEB26E273839A176@UWHC-MAIL01.uwhis.hosp.wisc.edu> <9EFD9EE5-92FA-4705-B5BB-2B5860A45D55@mac.com> Message-ID: <000601cbb1ba$c2399c60$6a03a8c0@apg> What is the purpose of batching control? Unless you are having a hard time finding certain positive tissue types, it's good practice to run a positive control with each case to ensure every slide shows the desired results. Plus the patholigist have to call and verify that the control worked, wasting their time. It doesn't waste money if you are attaching the patient tissue to your control tissue, and the few that require a separate control is just part of good patient care. We have been around and around about this subject in places I have worked and in the end batch controling is not the direction we want to go for patient care. If you are working in research enviroment maybe that is fine but if it was my specimen, run a control. Just my two cents! Thanks, Timothy Higgins, HT(ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ancillarypath@mac.com Sent: Tuesday, January 11, 2011 11:57 AM To: Sebree Linda A Cc: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com; Taylor,Jean Subject: Re: [IHCRG] RE: [Histonet] batching controls I agree with Linda. One positive control per *batch*, not per case. The positive control tissue can be placed on one of the patient's specimens, which saves you money on the detection system and antibody as well. We should be careful also about treating different drawers with different runs (such as the Bond, for example) as a separate batch, which requires its own set of controls. Some antibodies that have internal controls on every patient tissue do not require external controls, such as vimentin, CD31, CD34, etc. Predictive markers positive controls should always be the same controls, and can only be changed after verifying that the new control is okay to run and give you the desired signal. Thanks, Hadi Yaziji ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories 7480 SW 40 St, Suite 700 Miami, FL 33155 Tel 305-267-7979 F. 786-513-0175 www.vitromolecular.com On Jan 11, 2011, at 12:42 PM, Sebree Linda A wrote: > Hi Jean, > > We run one positive control for any given antibody that is ordered on > multiple cases and all are run at the same time on the same instrument. > Whenever possible that control has the patient tissue of one of the > cases on it. That case is referenced for the pathologists reading the > other cases with that antibody so they can find the positive control > if they feel the need. > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Taylor, Jean > Sent: Tuesday, January 11, 2011 11:22 AM > To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] batching controls > > Hi Everyone, > > I'd like to know how many labs batch their controls when the same > antibody is ordered on multiple cases. Do you run the control on a > separate slide, or with one of the cases ordered? > > Thank you! > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com To > unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtaylor <@t> meriter.com Tue Jan 11 12:30:08 2011 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Tue Jan 11 12:30:13 2011 Subject: [Histonet] batching controls In-Reply-To: Message-ID: I want to thank everyone for their input. After reading through the CAP guidelines, it sounds like both ways are acceptable as long as proper documentation is recorded. Thank you!! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Tuesday, January 11, 2011 12:16 PM To: Galbraith, Joe; Rathborne, Toni; Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We run 1 control per antibody within a case, so if we had 3 mart-1 on 3 separate cases I would run a control for each case Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor, Safety Officer, Physicians Laboratory Services Omaha, NE. 68127 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Tuesday, January 11, 2011 12:04 PM To: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 11, 2011 11:44 AM To: Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls How does this work if you need to send the case out for a consult and the control is on another patient's slide? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Tuesday, January 11, 2011 12:39 PM To: Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We batch our controls. We still try to put the control tissue on a patient slide, and then we reference that case that has the control on the other cases that don't have a control. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 9:22 AM To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From joseph-galbraith <@t> uiowa.edu Tue Jan 11 12:51:11 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Tue Jan 11 12:51:15 2011 Subject: [IHCRG] RE: [Histonet] batching controls In-Reply-To: References: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: <6DC87DEA9229894DB3A09F8B61717A46A74E@hc-mailboxc1-n3.healthcare.uiowa.edu> Hadi: Just for clarification, the control is on the same slide as the patient so it is subjected to the same conditions as the patient (post sectioning). The control block from which the control is cut is QA'ed against other known positive blocks prior to use as a control. Thanks. Joe -----Original Message----- From: ancillarypath@mac.com [mailto:ancillarypath@mac.com] Sent: Tuesday, January 11, 2011 12:09 PM To: Galbraith, Joe Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: Re: [IHCRG] RE: [Histonet] batching controls This is the most cautious approach, and for antibodies where there is no internal controls that would be your only way to verify that the studies worked. But it's also associated with some unnecessary rejection of good studies because controls aren't treated the same way as the patient slides. Good approach and we do it on our "technical-only" cases where the slides are returned to the outside pathologists for interpretation. Hadi On Jan 11, 2011, at 1:03 PM, Galbraith, Joe wrote: > We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. > > Joe > joseph-galbraith@uiowa.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni > Sent: Tuesday, January 11, 2011 11:44 AM > To: Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] batching controls > > How does this work if you need to send the case out for a consult and the control is on another patient's slide? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie > Colbert > Sent: Tuesday, January 11, 2011 12:39 PM > To: Taylor, Jean; ihcrg@googlegroups.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] batching controls > > > We batch our controls. We still try to put the control tissue on a > patient slide, and then we reference that case that has the control on > the other cases that don't have a control. > > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, > Jean > Sent: Tuesday, January 11, 2011 9:22 AM > To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] batching controls > > Hi Everyone, > > I'd like to know how many labs batch their controls when the same > antibody is ordered on multiple cases. Do you run the control on a > separate slide, or with one of the cases ordered? > > Thank you! > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From ancillarypath <@t> mac.com Tue Jan 11 13:07:05 2011 From: ancillarypath <@t> mac.com (Hadi Yaziji) Date: Tue Jan 11 13:07:28 2011 Subject: [IHCRG] RE: [Histonet] batching controls In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46A74E@hc-mailboxc1-n3.healthcare.uiowa.edu> References: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> <6DC87DEA9229894DB3A09F8B61717A46A74E@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: As you guessed correctly, I wasn't referring to analytical conditions. Control tissues are often fixed at longer times than patient tissues. Secondly, the surface in the control tissue blocks is often oxidized. Also, when control slides are cut, some labs cut dozens of controls from the same block and store the slides. All of these conditions make the control slides lose much of the sensitivity depending on the different parameters mentioned above. That is why in our consensus articles published in AIMM in 2007 and 2008, we insisted to put benign breast tissue in the same tumor section to make sure the "positive controls" are treated identically to the patient tissue. This is the only way to ensure the controls are treated the same way as patient tissue. What you correctly referred to is only a small part of the whole picture. Hadi On Jan 11, 2011, at 1:51 PM, Galbraith, Joe wrote: > Hadi: > > Just for clarification, the control is on the same slide as the patient so it is subjected to the same conditions as the patient (post sectioning). The control block from which the control is cut is QA'ed against other known positive blocks prior to use as a control. Thanks. > > Joe > > -----Original Message----- > From: ancillarypath@mac.com [mailto:ancillarypath@mac.com] > Sent: Tuesday, January 11, 2011 12:09 PM > To: Galbraith, Joe > Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu > Subject: Re: [IHCRG] RE: [Histonet] batching controls > > This is the most cautious approach, and for antibodies where there is no internal controls that would be your only way to verify that the studies worked. But it's also associated with some unnecessary rejection of good studies because controls aren't treated the same way as the patient slides. > > Good approach and we do it on our "technical-only" cases where the slides are returned to the outside pathologists for interpretation. > > Hadi > > On Jan 11, 2011, at 1:03 PM, Galbraith, Joe wrote: > >> We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. >> >> Joe >> joseph-galbraith@uiowa.edu >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni >> Sent: Tuesday, January 11, 2011 11:44 AM >> To: Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] batching controls >> >> How does this work if you need to send the case out for a consult and the control is on another patient's slide? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie >> Colbert >> Sent: Tuesday, January 11, 2011 12:39 PM >> To: Taylor, Jean; ihcrg@googlegroups.com; >> histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] batching controls >> >> >> We batch our controls. We still try to put the control tissue on a >> patient slide, and then we reference that case that has the control on >> the other cases that don't have a control. >> >> Laurie Colbert >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, >> Jean >> Sent: Tuesday, January 11, 2011 9:22 AM >> To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' >> Subject: [Histonet] batching controls >> >> Hi Everyone, >> >> I'd like to know how many labs batch their controls when the same >> antibody is ordered on multiple cases. Do you run the control on a >> separate slide, or with one of the cases ordered? >> >> Thank you! >> >> Jean Taylor, HT(ASCP)QIHC >> IHC Tech >> Meriter Health Services >> Madison, WI >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> CONFIDENTIALITY NOTICE >> This message and any included attachments are from Somerset Medical Center >> and are intended only for the addressee. The information contained in this >> message is confidential and may contain privileged, confidential, >> proprietary and/or trade secret information entitled to protection and/or >> exemption from disclosure under applicable law. Unauthorized forwarding, >> printing, copying, distribution, or use of such information is strictly >> prohibited and may be unlawful. If you are not the addressee, please >> promptly delete this message and notify the sender of the delivery error >> by e-mail or you may call Somerset Medical Center's computer Help Desk >> at 908-685-2200, ext. 4050. >> >> Be sure to visit Somerset Medical Center's Web site - >> www.somersetmedicalcenter.com - for the most up-to-date news, >> event listings, health information and more. >> >> -- >> You received this message because you are subscribed to the Google >> Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. >> >> To post to this group, send email to ihcrg@googlegroups.com >> To unsubscribe from this group, send email to >> ihcrg+unsubscribe@googlegroups.com >> For more options, visit this group at >> http://groups.google.com/group/ihcrg?hl=en >> >> To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. > From histology <@t> medsurgpath.com Tue Jan 11 13:17:48 2011 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Tue Jan 11 13:17:52 2011 Subject: [Histonet] batching controls In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu > References: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: We do the same. Control tissue on every slide. Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > We run an appropriate positive control on every patient test slide to test > that the antibody and all components used on that slide worked. This may > be overkill but it helps us ensure that each slide worked properly not > just the run. > > Joe > joseph-galbraith@uiowa.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, > Toni > Sent: Tuesday, January 11, 2011 11:44 AM > To: Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] batching controls > > How does this work if you need to send the case out for a consult and the > control is on another patient's slide? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie > Colbert > Sent: Tuesday, January 11, 2011 12:39 PM > To: Taylor, Jean; ihcrg@googlegroups.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] batching controls > > > We batch our controls. We still try to put the control tissue on a > patient slide, and then we reference that case that has the control on > the other cases that don't have a control. > > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, > Jean > Sent: Tuesday, January 11, 2011 9:22 AM > To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] batching controls > > Hi Everyone, > > I'd like to know how many labs batch their controls when the same > antibody is ordered on multiple cases. Do you run the control on a > separate slide, or with one of the cases ordered? > > Thank you! > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joseph-galbraith <@t> uiowa.edu Tue Jan 11 13:29:19 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Tue Jan 11 13:29:24 2011 Subject: [IHCRG] RE: [Histonet] batching controls In-Reply-To: References: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> <6DC87DEA9229894DB3A09F8B61717A46A74E@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: <6DC87DEA9229894DB3A09F8B61717A46A789@hc-mailboxc1-n3.healthcare.uiowa.edu> Hadi: I concur with your comments completely. We attempt to make the conditions the same to the extent possible but it is never exact. Facing the block should cut away surface oxidation in most cases. We avoid precutting large numbers of controls (particularly for antibodies known to be particularly sensitive to tissue oxidation where we will cut the control on the same day, often at the same time, as the patient, taking care to avoid cross contamination). There are so many variables to consider. Testing for oxidative decay should probably be standard operating procedure for control block evaluation especially for new antibodies. Internal controls are always the best from this standpoint. Best wishes. Joe -----Original Message----- From: Hadi Yaziji [mailto:ancillarypath@mac.com] Sent: Tuesday, January 11, 2011 1:07 PM To: Galbraith, Joe Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: Re: [IHCRG] RE: [Histonet] batching controls As you guessed correctly, I wasn't referring to analytical conditions. Control tissues are often fixed at longer times than patient tissues. Secondly, the surface in the control tissue blocks is often oxidized. Also, when control slides are cut, some labs cut dozens of controls from the same block and store the slides. All of these conditions make the control slides lose much of the sensitivity depending on the different parameters mentioned above. That is why in our consensus articles published in AIMM in 2007 and 2008, we insisted to put benign breast tissue in the same tumor section to make sure the "positive controls" are treated identically to the patient tissue. This is the only way to ensure the controls are treated the same way as patient tissue. What you correctly referred to is only a small part of the whole picture. Hadi On Jan 11, 2011, at 1:51 PM, Galbraith, Joe wrote: > Hadi: > > Just for clarification, the control is on the same slide as the patient so it is subjected to the same conditions as the patient (post sectioning). The control block from which the control is cut is QA'ed against other known positive blocks prior to use as a control. Thanks. > > Joe > > -----Original Message----- > From: ancillarypath@mac.com [mailto:ancillarypath@mac.com] > Sent: Tuesday, January 11, 2011 12:09 PM > To: Galbraith, Joe > Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu > Subject: Re: [IHCRG] RE: [Histonet] batching controls > > This is the most cautious approach, and for antibodies where there is no internal controls that would be your only way to verify that the studies worked. But it's also associated with some unnecessary rejection of good studies because controls aren't treated the same way as the patient slides. > > Good approach and we do it on our "technical-only" cases where the slides are returned to the outside pathologists for interpretation. > > Hadi > > On Jan 11, 2011, at 1:03 PM, Galbraith, Joe wrote: > >> We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. >> >> Joe >> joseph-galbraith@uiowa.edu >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni >> Sent: Tuesday, January 11, 2011 11:44 AM >> To: Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] batching controls >> >> How does this work if you need to send the case out for a consult and the control is on another patient's slide? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie >> Colbert >> Sent: Tuesday, January 11, 2011 12:39 PM >> To: Taylor, Jean; ihcrg@googlegroups.com; >> histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] batching controls >> >> >> We batch our controls. We still try to put the control tissue on a >> patient slide, and then we reference that case that has the control on >> the other cases that don't have a control. >> >> Laurie Colbert >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, >> Jean >> Sent: Tuesday, January 11, 2011 9:22 AM >> To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' >> Subject: [Histonet] batching controls >> >> Hi Everyone, >> >> I'd like to know how many labs batch their controls when the same >> antibody is ordered on multiple cases. Do you run the control on a >> separate slide, or with one of the cases ordered? >> >> Thank you! >> >> Jean Taylor, HT(ASCP)QIHC >> IHC Tech >> Meriter Health Services >> Madison, WI >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> CONFIDENTIALITY NOTICE >> This message and any included attachments are from Somerset Medical Center >> and are intended only for the addressee. The information contained in this >> message is confidential and may contain privileged, confidential, >> proprietary and/or trade secret information entitled to protection and/or >> exemption from disclosure under applicable law. Unauthorized forwarding, >> printing, copying, distribution, or use of such information is strictly >> prohibited and may be unlawful. If you are not the addressee, please >> promptly delete this message and notify the sender of the delivery error >> by e-mail or you may call Somerset Medical Center's computer Help Desk >> at 908-685-2200, ext. 4050. >> >> Be sure to visit Somerset Medical Center's Web site - >> www.somersetmedicalcenter.com - for the most up-to-date news, >> event listings, health information and more. >> >> -- >> You received this message because you are subscribed to the Google >> Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. >> >> To post to this group, send email to ihcrg@googlegroups.com >> To unsubscribe from this group, send email to >> ihcrg+unsubscribe@googlegroups.com >> For more options, visit this group at >> http://groups.google.com/group/ihcrg?hl=en >> >> To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. > From kgrobert <@t> rci.rutgers.edu Tue Jan 11 13:49:31 2011 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Tue Jan 11 13:49:36 2011 Subject: [Histonet] Chatter or waviness of sections under the 'scope Message-ID: <15eaf7fd91edf8ece5ef840aff5d9f5d.squirrel@webmail.rci.rutgers.edu> To all, I am having a problem with either chatter or waviness of my sections when I look at them under the microscope. I've had chatter before, but in these sections, the chattered areas of tissue are literally standing up, giving the section a 3D look. The waviness is the same thing, except the tissue is in one piece-a 3D appearance under the microscope, no matter what tissue I have cut. (It kind of looks to me like the way bacon curls up in the pan when it's cooked-the fat in the bacon curls up and the meat mostly remains flat.) So far I have seen it in mouse kidney, liver and trachea. Have my Ultrastick slides lost their charge, is it static electricity, bad infiltration, or something else? If you need to see a picture,let me know & I'll go take one & post it. Thank you so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 From Rcartun <@t> harthosp.org Tue Jan 11 14:01:01 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jan 11 14:01:14 2011 Subject: [Histonet] Re: [IHCRG] batching controls In-Reply-To: References: Message-ID: <4D2C70AC.7400.0077.1@harthosp.org> We use "batch" controls; one for every antibody (per machine) regardless of how many patient test slides there are. We do not put the positive control tissue on the test slide since we use a lot of unstained slides that have been cut as part of a histology protocol when the H&E slides are prepared. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Taylor, Jean" 1/11/2011 12:21 PM >>> Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From Rcartun <@t> harthosp.org Tue Jan 11 14:07:17 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jan 11 14:07:26 2011 Subject: [IHCRG] RE: [Histonet] batching controls In-Reply-To: <000601cbb1ba$c2399c60$6a03a8c0@apg> References: <8C023B4AB999614BA4791BAEB26E273839A176@UWHC-MAIL01.uwhis.hosp.wisc.edu> <9EFD9EE5-92FA-4705-B5BB-2B5860A45D55@mac.com> <000601cbb1ba$c2399c60$6a03a8c0@apg> Message-ID: <4D2C7224.7400.0077.1@harthosp.org> No slides should be given to a pathologist until the positive controls have been reviewed and appropriately documented as "Satisfactory" by the Director of the laboratory or her/his designee. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Tim Higgins" 1/11/2011 1:09 PM >>> What is the purpose of batching control? Unless you are having a hard time finding certain positive tissue types, it's good practice to run a positive control with each case to ensure every slide shows the desired results. Plus the patholigist have to call and verify that the control worked, wasting their time. It doesn't waste money if you are attaching the patient tissue to your control tissue, and the few that require a separate control is just part of good patient care. We have been around and around about this subject in places I have worked and in the end batch controling is not the direction we want to go for patient care. If you are working in research enviroment maybe that is fine but if it was my specimen, run a control. Just my two cents! Thanks, Timothy Higgins, HT(ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ancillarypath@mac.com Sent: Tuesday, January 11, 2011 11:57 AM To: Sebree Linda A Cc: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com; Taylor,Jean Subject: Re: [IHCRG] RE: [Histonet] batching controls I agree with Linda. One positive control per *batch*, not per case. The positive control tissue can be placed on one of the patient's specimens, which saves you money on the detection system and antibody as well. We should be careful also about treating different drawers with different runs (such as the Bond, for example) as a separate batch, which requires its own set of controls. Some antibodies that have internal controls on every patient tissue do not require external controls, such as vimentin, CD31, CD34, etc. Predictive markers positive controls should always be the same controls, and can only be changed after verifying that the new control is okay to run and give you the desired signal. Thanks, Hadi Yaziji ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories 7480 SW 40 St, Suite 700 Miami, FL 33155 Tel 305-267-7979 F. 786-513-0175 www.vitromolecular.com On Jan 11, 2011, at 12:42 PM, Sebree Linda A wrote: > Hi Jean, > > We run one positive control for any given antibody that is ordered on > multiple cases and all are run at the same time on the same instrument. > Whenever possible that control has the patient tissue of one of the > cases on it. That case is referenced for the pathologists reading the > other cases with that antibody so they can find the positive control > if they feel the need. > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Taylor, Jean > Sent: Tuesday, January 11, 2011 11:22 AM > To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] batching controls > > Hi Everyone, > > I'd like to know how many labs batch their controls when the same > antibody is ordered on multiple cases. Do you run the control on a > separate slide, or with one of the cases ordered? > > Thank you! > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com To > unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Jan 11 14:43:31 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jan 11 14:43:40 2011 Subject: [IHCRG] RE: [Histonet] batching controls In-Reply-To: References: <57BE698966D5C54EAE8612E8941D76830A50AE92@EXCHANGE3.huntingtonhospital.com> <6DC87DEA9229894DB3A09F8B61717A46A6CD@hc-mailboxc1-n3.healthcare.uiowa.edu> <6DC87DEA9229894DB3A09F8B61717A46A74E@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: <4D2C7AA2.7400.0077.1@harthosp.org> Hadi makes an excellent point about different fixation times for patient specimens and tissue used for positive controls. This is one reason why I don't want the positive control tissue on the patient slide because I frequently "personalize" the antigen retrieval for each tissue (longer antigen retrieval for positive control tissue; less for patient tissue). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Hadi Yaziji 1/11/2011 2:07 PM >>> As you guessed correctly, I wasn't referring to analytical conditions. Control tissues are often fixed at longer times than patient tissues. Secondly, the surface in the control tissue blocks is often oxidized. Also, when control slides are cut, some labs cut dozens of controls from the same block and store the slides. All of these conditions make the control slides lose much of the sensitivity depending on the different parameters mentioned above. That is why in our consensus articles published in AIMM in 2007 and 2008, we insisted to put benign breast tissue in the same tumor section to make sure the "positive controls" are treated identically to the patient tissue. This is the only way to ensure the controls are treated the same way as patient tissue. What you correctly referred to is only a small part of the whole picture. Hadi On Jan 11, 2011, at 1:51 PM, Galbraith, Joe wrote: > Hadi: > > Just for clarification, the control is on the same slide as the patient so it is subjected to the same conditions as the patient (post sectioning). The control block from which the control is cut is QA'ed against other known positive blocks prior to use as a control. Thanks. > > Joe > > -----Original Message----- > From: ancillarypath@mac.com [mailto:ancillarypath@mac.com] > Sent: Tuesday, January 11, 2011 12:09 PM > To: Galbraith, Joe > Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu > Subject: Re: [IHCRG] RE: [Histonet] batching controls > > This is the most cautious approach, and for antibodies where there is no internal controls that would be your only way to verify that the studies worked. But it's also associated with some unnecessary rejection of good studies because controls aren't treated the same way as the patient slides. > > Good approach and we do it on our "technical-only" cases where the slides are returned to the outside pathologists for interpretation. > > Hadi > > On Jan 11, 2011, at 1:03 PM, Galbraith, Joe wrote: > >> We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. >> >> Joe >> joseph-galbraith@uiowa.edu >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni >> Sent: Tuesday, January 11, 2011 11:44 AM >> To: Laurie Colbert; Taylor, Jean; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] batching controls >> >> How does this work if you need to send the case out for a consult and the control is on another patient's slide? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie >> Colbert >> Sent: Tuesday, January 11, 2011 12:39 PM >> To: Taylor, Jean; ihcrg@googlegroups.com; >> histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] batching controls >> >> >> We batch our controls. We still try to put the control tissue on a >> patient slide, and then we reference that case that has the control on >> the other cases that don't have a control. >> >> Laurie Colbert >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, >> Jean >> Sent: Tuesday, January 11, 2011 9:22 AM >> To: 'ihcrg@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' >> Subject: [Histonet] batching controls >> >> Hi Everyone, >> >> I'd like to know how many labs batch their controls when the same >> antibody is ordered on multiple cases. Do you run the control on a >> separate slide, or with one of the cases ordered? >> >> Thank you! >> >> Jean Taylor, HT(ASCP)QIHC >> IHC Tech >> Meriter Health Services >> Madison, WI >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> CONFIDENTIALITY NOTICE >> This message and any included attachments are from Somerset Medical Center >> and are intended only for the addressee. The information contained in this >> message is confidential and may contain privileged, confidential, >> proprietary and/or trade secret information entitled to protection and/or >> exemption from disclosure under applicable law. Unauthorized forwarding, >> printing, copying, distribution, or use of such information is strictly >> prohibited and may be unlawful. If you are not the addressee, please >> promptly delete this message and notify the sender of the delivery error >> by e-mail or you may call Somerset Medical Center's computer Help Desk >> at 908-685-2200, ext. 4050. >> >> Be sure to visit Somerset Medical Center's Web site - >> www.somersetmedicalcenter.com - for the most up-to-date news, >> event listings, health information and more. >> >> -- >> You received this message because you are subscribed to the Google >> Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. >> >> To post to this group, send email to ihcrg@googlegroups.com >> To unsubscribe from this group, send email to >> ihcrg+unsubscribe@googlegroups.com >> For more options, visit this group at >> http://groups.google.com/group/ihcrg?hl=en >> >> To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. > -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From kimd <@t> slonepartners.com Tue Jan 11 14:55:28 2011 From: kimd <@t> slonepartners.com (Kim Wilson) Date: Tue Jan 11 14:55:41 2011 Subject: [Histonet] histonet mailing Message-ID: <84040C93-9AB1-4444-89B9-5BDC2EF7C4B9@slonepartners.com> Can you please post this on the histonet mailing list? Thank you! Kim Slone Partners seeks a Histotechnologist for a cutting edge hospital laboratory, based in a beautiful Washington DC neighborhood. ASCP certification is preferred with at least 2 years of experience in a high-volume laboratory. Special features of the position: This organization embraces curiosity and enjoys mentoring and developing its employees. Interested and qualified candidates should submit their resume to Kim Wilson at kimd@slonepartners.com. SLONEPARTNERS KIM WILSON - EXECUTIVE RECRUITER FORT WORTH, TX Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877-436-8105 DIRECT: 817-595-2227 KIMD@SLONEPARTNERS.COM SLONEPARTNERS.COM The information contained is privileged and confidential information owned by Slone Partners and is only for the individual or entry named above. If you receive this in error please discard-do not transmit further. From pdunlop720 <@t> gmail.com Tue Jan 11 15:04:17 2011 From: pdunlop720 <@t> gmail.com (Patty Dunlop) Date: Tue Jan 11 15:04:21 2011 Subject: [Histonet] paraffin carry-over problem Message-ID: Hello, I have a problem with what appears to be flecks of paraffin coming off of my slides in my first change of alcohol in my H&E stain. My pathologist indicates seeing "drops of unstained areas on the tissue", and I suspect it is from unsatisfactory deparaffinization. I use clear-rite 3 xylene subst. and have always done 3 changes at 3 min each before going to 100% alcohol. I have contacted the company and they claim that nothing is wrong with the product. I have tried changing out all stations to fresh clear rite 3 and increasing the time to 5 min each. I have also increased the temp on my slide oven (65 for 20min). All to no avail. Could this not be the problem? Could it be the paraffin? I use paraplast plus. I would love to hear ANY suggestions other than what I have already tried! I'm starting to really worry now since the problem has persisted for a few months. Thank you! Patty From Bonnie.Whitaker <@t> osumc.edu Tue Jan 11 16:00:11 2011 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Tue Jan 11 16:00:10 2011 Subject: [Histonet] Histology Lab Manager--Ohio State University Med Center Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670152C14390@EXMBOX-VP05.OSUMC.EDU> Hi Everyone, The position of Clinical Histology Laboratory Manager here at The Ohio State University Medical Center is still open: We are located in Columbus, OH which is a wonderful city with a population of 1.75 million. We are the 16th largest city in the US, without the traffic problems of some of the larger cities. There are 19 communities within 30 minutes of downtown Columbus. It really is a lovely area, complete with four seasons. The histology lab is a good lab, with a stable, hard-working staff. Our average block count is about 765/day and we have about 18 techs in histology. Also, we have great benefits!! Here is the official blurb: The OSUMC clinical histology laboratory is a functional laboratory within the Anatomic Pathology branch serving the pathology subspecialty divisions. The Manager will provide leadership for daily operations of the histology labs, manages human resources, financial and budget planning and preparation, new test development, quality improvement, safety programs, compliance program, successful inspection and accreditation processes, and ensuring current policies and procedures related to technical, operational, and administrative areas of the laboratory. For a complete position description and application instructions please go to http://medicalcenter.osu.edu/careers/ and search by posting number 354362. The Ohio State University is an equal opportunity, affirmative active employer. Women, minorities, veterans, and individuals with disabilities are encouraged to apply. Please don't hesitate to give me a call, or email me if you want more information. Thanks! Bonnie Whitaker AP Operations Director Ohio State University Medical Center Anatomic Pathology Branch Department of Pathology N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 From rlhenshall_powell <@t> yahoo.co.uk Tue Jan 11 17:18:45 2011 From: rlhenshall_powell <@t> yahoo.co.uk (Rhonda Henshall-Powell) Date: Tue Jan 11 17:18:49 2011 Subject: [Histonet] Need help with p16 pricing Message-ID: <403780.58144.qm@web29517.mail.ird.yahoo.com> Hello All, I am having a hard time getting list price on MTM's p16 CINtec kit for histology - anyone using is out there? Is this the only IVD test on the market? What are people using? is it cost effective? CINtec? Histology: Part No. 9517 Thanks, Rhonda From Kimberly.Blundon <@t> drdc-rddc.gc.ca Tue Jan 11 17:19:28 2011 From: Kimberly.Blundon <@t> drdc-rddc.gc.ca (Blundon, Kimberly) Date: Tue Jan 11 17:19:32 2011 Subject: [Histonet] Thank you Message-ID: <42DFE1A029181B4B8CCBA7261B52D7656B0E56@suffieldex01.suffield.drdc-rddc.gc.ca> Thanks to those who replied to my cryostat help. It was very helpful! Thanks again! Kimberly From robin_dean <@t> compbio.com Tue Jan 11 17:29:47 2011 From: robin_dean <@t> compbio.com (Robin Dean) Date: Tue Jan 11 17:32:49 2011 Subject: [Histonet] CD4 antibody for frozen mouse tissue Message-ID: <004801cbb1e7$6fc89850$4f59c8f0$@com> Can anyone recommend a good CD4 antibody for use in localizing CD4 in frozen mouse tissue? We are being asked to do dual immunofluorescent (IHC) labeling with the CD4 and another antibody in frozen mouse tissue. I know CD4 localization in mouse tissue is difficult and would appreciate any help or guidance anyone might have to offer. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com From A.Mortimer <@t> latrobe.edu.au Tue Jan 11 19:44:45 2011 From: A.Mortimer <@t> latrobe.edu.au (Abbey Mortimer) Date: Tue Jan 11 19:44:53 2011 Subject: [Histonet] Microglia/ macrophage staining Message-ID: Hello everybody, I am wondering if anybody has advice on a good antibody to use to stain for/ distinguish microglia vs macrophages in rat brain (frozen tissue/ mounted in OCT, and cut on cryostat). It is my understanding that this can be a complicated issue. Any help would be greatly appreciated! Thanks Abbey From Mark.Elliott <@t> hli.ubc.ca Tue Jan 11 22:30:13 2011 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Tue Jan 11 22:30:55 2011 Subject: [Histonet] Processing paraffin embedded samples for EM Message-ID: <4D2CBDD5020000D600059F58@mail.mrl.ubc.ca> We have been asked to take tissue that has been FFPE and deparafinize and process for EM. Has anyone done this? Any tips on what to do/not do? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Fawn.Bomar <@t> HalifaxRegional.com Wed Jan 12 08:33:06 2011 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Wed Jan 12 08:33:16 2011 Subject: [Histonet] Thyroid Smears Message-ID: Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From rjbuesa <@t> yahoo.com Wed Jan 12 08:38:50 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 12 08:38:53 2011 Subject: [Histonet] Thyroid Smears In-Reply-To: Message-ID: <206027.5027.qm@web65711.mail.ac4.yahoo.com> Tell the pathologist to stop fixing the smears. Let the smears air dry and later you fix/stain them. Besides fixing with 2-propanol is not the way to fix. If they also dismiss this procedure, then?the will end without stained smears. Ren? J. --- On Wed, 1/12/11, Fawn Bomar wrote: From: Fawn Bomar Subject: [Histonet] Thyroid Smears To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 12, 2011, 9:33 AM Hello Everyone! Happy New Years to all!? I have a question regarding the preparation of thyroid smears.? As of right now, we go up to the room and collect the thyroid sample.? The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix.? We then complete the stain later on in the day.? The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain.? Does anyone have any suggestions or are willing to share the procedure that they use?? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged.? It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Wed Jan 12 08:45:05 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Jan 12 08:45:19 2011 Subject: [Histonet] paraffin carry-over problem In-Reply-To: References: Message-ID: I had never used clear rite before, but in coming to this lab it is what they use because they don't want that much xylene around. However, we do still have xylene in the lab. I thought the problem you describe was just a product of the clear rite so my solution...the first change after the oven is xylene. Shh, don't tell on me =) This has made the bubbling (lack of staining) and the paraffin in the alcohol go away. If you can try this, it seems to work? Also, I have my oven set to about 75C for 15 or 20 minutes. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patty Dunlop Sent: Tuesday, January 11, 2011 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin carry-over problem Hello, I have a problem with what appears to be flecks of paraffin coming off of my slides in my first change of alcohol in my H&E stain. My pathologist indicates seeing "drops of unstained areas on the tissue", and I suspect it is from unsatisfactory deparaffinization. I use clear-rite 3 xylene subst. and have always done 3 changes at 3 min each before going to 100% alcohol. I have contacted the company and they claim that nothing is wrong with the product. I have tried changing out all stations to fresh clear rite 3 and increasing the time to 5 min each. I have also increased the temp on my slide oven (65 for 20min). All to no avail. Could this not be the problem? Could it be the paraffin? I use paraplast plus. I would love to hear ANY suggestions other than what I have already tried! I'm starting to really worry now since the problem has persisted for a few months. Thank you! Patty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Wed Jan 12 08:50:13 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Jan 12 08:50:22 2011 Subject: [Histonet] Thyroid Smears In-Reply-To: References: Message-ID: We always used 95% alcohol to fix, but you're right the blood usually comes off. Air dry then diff quik (or giemsa) stain works better for a hema-pathologic stain. You could try Pen Fix (or an alcoholic formalin) and this might help as opposed to the 95%. Why does the pathologist need to see blood cells in a thyroid asp.? Just my 2 cents =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, January 12, 2011 8:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thyroid Smears Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Jan 12 08:53:56 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jan 12 08:54:04 2011 Subject: [Histonet] Need help with p16 pricing In-Reply-To: <403780.58144.qm@web29517.mail.ird.yahoo.com> References: <403780.58144.qm@web29517.mail.ird.yahoo.com> Message-ID: <4D2D7A33.7400.0077.1@harthosp.org> We use the mtm "predilute" mAb with excellent results. Due to the sensitivity of our detection (Leica-Microsystems' Bond Polymer Refine kit), we are able to dilute it to 1:10 and still obtain outstanding immunoreactivity. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Rhonda Henshall-Powell 1/11/2011 6:18 PM >>> Hello All, I am having a hard time getting list price on MTM's p16 CINtec kit for histology - anyone using is out there? Is this the only IVD test on the market? What are people using? is it cost effective? CINtec? Histology: Part No. 9517 Thanks, Rhonda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Wed Jan 12 09:00:02 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Jan 12 09:00:10 2011 Subject: [Histonet] Thyroid Smears In-Reply-To: Message-ID: Our procedure is as such: The FNA of the thyroid is place in 5mls of plasmalyte ( an electrolyte solution). We lyse the red blood cells and make cytospins. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Fawn Bomar Sent by: histonet-bounces@lists.utsouthwestern.edu 01/12/2011 09:37 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Thyroid Smears Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Beatrice.Debrosse-Serra <@t> pfizer.com Wed Jan 12 10:10:16 2011 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Wed Jan 12 10:10:49 2011 Subject: [Histonet] CD4 antibody for frozen mouse tissue In-Reply-To: <004801cbb1e7$6fc89850$4f59c8f0$@com> References: <004801cbb1e7$6fc89850$4f59c8f0$@com> Message-ID: Hi Robin, We are using a biotinylated Anti-mouse CD4 from R&D Systems with great success on frozen mouse tissue for IHC. The catalog number is BAM554. We haven't used it in IF, but I would imagine it should work just as well for multiplexing. Let me know if you need any more info. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Investigative Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Dean Sent: Tuesday, January 11, 2011 3:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD4 antibody for frozen mouse tissue Can anyone recommend a good CD4 antibody for use in localizing CD4 in frozen mouse tissue? We are being asked to do dual immunofluorescent (IHC) labeling with the CD4 and another antibody in frozen mouse tissue. I know CD4 localization in mouse tissue is difficult and would appreciate any help or guidance anyone might have to offer. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Aubrey <@t> nsh.org Wed Jan 12 10:32:48 2011 From: Aubrey <@t> nsh.org (Aubrey Wanner) Date: Wed Jan 12 10:32:55 2011 Subject: [Histonet] Registration is now open for the NSH Forum on Troubleshooting Stains Message-ID: NSH has recruited two of its highest rated speakers to bring you this one day forum dedicated to troubleshooting stains. New and seasoned Histotechs will benefit from their insights and handout material. Special for this event - handouts will be available for download in color following the event. The event will take place February 19, 2011 in Bethesda, MD, 8am - 4:30pm. Individuals can register via the NSH website, http://www.nsh.org/stainforum Topics include: AM Session: Troubleshooting Hematoxylin and Eosin Staining Presented by Ada Feldman, MS, HT/HTL(ASCP)CM, Anatech Ltd, Battle Creek, MI Hematoxylin and eosin is the primary diagnostic stain in anatomical pathology. However, tissue processing and staining procedure steps can all affect the H&E's final appearance, potentially interfering with diagnosis. This workshop will help the histotech troubleshoot such common problems of smudgy nuclei, improper staining, "soap suds", haziness and more. The workshop will review the steps in tissue processing (fixation through slide drying) and H&E staining (deparaffinization through coverslipping) that can alter the stained appearance of a slide. Traditional reagents (formalin, alcohol, xylene) as well as substitute reagents (xylene substitutes, special fixatives, formalin-free fixatives) are discussed. PM Session: Troubleshooting Immunohistochemical Stains Presented by Richard W. Cartun, MS, PhD, Hartford Hospital, Hartford, CT Results from immunohistochemical stains are essential for patient diagnosis and treatment. The major goal of standardization in immunohistochemical testing is to produce high-quality stains where the results are accurate and reproducible, both in your laboratory and in others. Do you find yourself repeating a lot of immunohistochemical stains due to high background, uneven immunoreactivity, weak immunoreactivity, or a negative result when your pathologist tells you it should be positive? This workshop will examine causes of suboptimal immunohistochemical staining including poor fixation, poor tissue sectioning, inadequate antigen retrieval, antibody dilution errors, and detection system problems. The importance of antibody validation, use of positive and negative controls, and quality assurance/quality control procedures will also be discussed. From irena.kirbis <@t> hotmail.com Wed Jan 12 10:55:22 2011 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Wed Jan 12 10:55:26 2011 Subject: [Histonet] Thyroid Smears In-Reply-To: References: Message-ID: I guess that you're staining these fix slides by Papanicolaou?, perhaps the smears are too thick?, we process daily different kind of smears and always prepare one fix and one air dry smear for Pap and Giemsa staining and occasionaly we have an issue only very muccous samples which can coming off but never bloody smears except if they are very thick. one simples way to get rid of blood from cell suspension is a filtration through nylon mesh with 20 microns pores (published in Cytopathology - Heamorrhagic cytology samples- how to get best out of it) Irena > From: Fawn.Bomar@HalifaxRegional.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 12 Jan 2011 09:33:06 -0500 > Subject: [Histonet] Thyroid Smears > > Hello Everyone! > > Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. > > Thank you in advance, > Fawn > ------------------------------------------------------------- > This electronic message may contain information that is > confidential or legally privileged. It is intended only > for the use of the individual(s) and entity named as recipients > in the message. > > If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from any > computer. Do not deliver, distribute, or copy this message, and > do not disclose its contents or take any action in reliance on > the information it contains. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sharon.Davis-Devine <@t> carle.com Wed Jan 12 11:02:03 2011 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Jan 12 11:02:08 2011 Subject: [Histonet] Thyroid Smears In-Reply-To: References: Message-ID: Try using plus slides and make the smears very thin. Then it won't matter what kind of fixative you are using everything will stick. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS Sent: Wednesday, January 12, 2011 10:55 AM To: fawn.bomar@halifaxregional.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thyroid Smears I guess that you're staining these fix slides by Papanicolaou?, perhaps the smears are too thick?, we process daily different kind of smears and always prepare one fix and one air dry smear for Pap and Giemsa staining and occasionaly we have an issue only very muccous samples which can coming off but never bloody smears except if they are very thick. one simples way to get rid of blood from cell suspension is a filtration through nylon mesh with 20 microns pores (published in Cytopathology - Heamorrhagic cytology samples- how to get best out of it) Irena > From: Fawn.Bomar@HalifaxRegional.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 12 Jan 2011 09:33:06 -0500 > Subject: [Histonet] Thyroid Smears > > Hello Everyone! > > Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. > > Thank you in advance, > Fawn > ------------------------------------------------------------- > This electronic message may contain information that is > confidential or legally privileged. It is intended only > for the use of the individual(s) and entity named as recipients > in the message. > > If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from any > computer. Do not deliver, distribute, or copy this message, and > do not disclose its contents or take any action in reliance on > the information it contains. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Jan 12 11:25:39 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Jan 12 11:25:46 2011 Subject: [Histonet] RE: Murine CD4 antibody for immunofluorescence staining Message-ID: <000c01cbb27d$bd50b3f0$37f21bd0$@callis@bresnan.net> Dear Robin, You Wrote: Can anyone recommend a good CD4 antibody for use in localizing CD4 in frozen mouse tissue? We are being asked to do dual immunofluorescent (IHC. labeling with the CD4 and another antibody in frozen mouse tissue. I know CD4 localization in mouse tissue is difficult and would appreciate any help or guidance anyone might have to offer. Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 ********** We use BD Bioscience rat antiMouse CD4 (L3T4) IgG2a isotype. Your negative control will be Rat IgG2a. You can also purchase this monoclonal antibody from eBioscience, or Serotec. We have not found working with murine CD4 difficult and have done triple immunofluroescence staining with this antibody in combination with CD8 and PNad. If you are working with two primary antibodies from the same host, eg. rat ant mouse CD4 and then rat antimouse CD8, you have to do sequential staining. You can do the staining two ways. Rat antiCD4 biotinylated and come back with Strepavidin Alexa dye. This gets rid of the secondary antibody but the negative control, Rat IgG 2a has to be biotinylated too. When we work with Streptavidin Alexa dyes, we do a Streptavidin/biotin block from Vector. OR you can Rat antiCD4 and detect with donkey antiRat Dylight 488, F(ab')2 frag of IgG from Jackson, adsorbed to mouse. Rat IgG2a is the negative control We use a special acetone/alcohol fixation on air dried frozen sections and at the end, Molecular Probes antifade reagent, ready to use mounting media to keep fluorophore from fading. I will be happy to send our protocol under private reply if you want it that works to make double immunofluorescence staining more efficient and background free, and will include a photo of the results. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From mtitford <@t> aol.com Wed Jan 12 12:02:35 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jan 12 12:02:55 2011 Subject: [Histonet] Deparaffinization for EM Message-ID: <8CD809A06C3774E-13C4-538@webmail-d023.sysops.aol.com> Mark Elliot asks about processing FFPE tissue for EM. Hayat published a method in his book we use. Briefly you dig out 1mm cube pieces of tissue from the block and rotate them in xylene for one hour, followed by absolute, 95%, 70%,50% alcohols for 15 minutes each and then into your EM buffer overnight, followed by your normal EM processing next day. The EM image looks pretty grotty but if your pathologist can make a diagnosis it is worth it. (Hayat M.A. Principles and techniques of electron microscopy. 2ed edition. Baltimore. University Park Press. 1981 page 209) In another method published years ago, someone by-passed the hydration steps by deparaffizing in osmium dissolved in xylene, then going straight to Epon. Hope this helps Michael Titford Pathology USA Mobile AL USA From LSetlak <@t> childrensmemorial.org Wed Jan 12 12:03:00 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Wed Jan 12 12:03:06 2011 Subject: [Histonet] Thyroid Smears In-Reply-To: References: Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7682DE@CMHEXCC01MBX.childrensmemorial.org> I agree with Sharon, that's what we use and never really have a problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, January 12, 2011 11:02 AM To: 'IRENA SREBOTNIK KIRBIS'; fawn.bomar@halifaxregional.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thyroid Smears Try using plus slides and make the smears very thin. Then it won't matter what kind of fixative you are using everything will stick. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS Sent: Wednesday, January 12, 2011 10:55 AM To: fawn.bomar@halifaxregional.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thyroid Smears I guess that you're staining these fix slides by Papanicolaou?, perhaps the smears are too thick?, we process daily different kind of smears and always prepare one fix and one air dry smear for Pap and Giemsa staining and occasionaly we have an issue only very muccous samples which can coming off but never bloody smears except if they are very thick. one simples way to get rid of blood from cell suspension is a filtration through nylon mesh with 20 microns pores (published in Cytopathology - Heamorrhagic cytology samples- how to get best out of it) Irena > From: Fawn.Bomar@HalifaxRegional.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 12 Jan 2011 09:33:06 -0500 > Subject: [Histonet] Thyroid Smears > > Hello Everyone! > > Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. > > Thank you in advance, > Fawn > ------------------------------------------------------------- > This electronic message may contain information that is > confidential or legally privileged. It is intended only > for the use of the individual(s) and entity named as recipients > in the message. > > If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from any > computer. Do not deliver, distribute, or copy this message, and > do not disclose its contents or take any action in reliance on > the information it contains. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Bell <@t> ucdenver.edu Wed Jan 12 12:23:02 2011 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Jan 12 12:23:09 2011 Subject: [Histonet] Alpha V Integrin Message-ID: <64DB27005E2FD3439E88502D7A5C91219EB99222F6@CORTEZ.ucdenver.pvt> Hello to Everyone and Happy New Year, Does anyone know of an antibody for Alpha V Integrin for IHC that will work in mouse tissue? Thank you for all of your help. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu From campbellj <@t> muhlbauerlab.com Wed Jan 12 12:35:25 2011 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Wed Jan 12 12:35:29 2011 Subject: [Histonet] coverglass Message-ID: I am looking for vendors for coverglass for an automated coverglasser. I want one where the coverglasses don't stick together. We are currently using Mercedes as our vendor. Any feedback out there for ThermoFisher? -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory P: 585.586.5166 F: 585.586.3137 From POWELL_SA <@t> mercer.edu Wed Jan 12 12:40:07 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Jan 12 12:40:14 2011 Subject: [Histonet] GSH meeting in March Message-ID: <9BF995BC0E47744E9673A41486E24EE22DB6BFC539@MERCERMAIL.MercerU.local> Hi Fellow Histonetters, The Georgia Society for Histotechnology wants to invite you to our meeting at Callaway Gardens, Pine Mountain GA March 25-27th, 2011. We had our meeting there in 2007 which had a wonderful program and turnout. This year should be the same. Our program can be downloaded by going to http://www.histosearch.com/gsh/symposium.html The vendor registration form can also be downloaded here too. Make your reservations early at the Mountain Creek Inn by calling 1-800-225-5292. The room rates are $99 which includes Continental Breakfast and Admission to the Park. Plan to join us in the beautiful gardens and enjoy the peaceful surroundings. make it part of your vacation, bring the family. The championship golf courses, the man-made beach, and the acres and acres of flowers, birds, plants, animals and the butterfly sanctuary are outstanding. Check them out at their website http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx today. Shirley Powell, GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From liz <@t> premierlab.com Wed Jan 12 12:56:51 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 12 12:57:02 2011 Subject: [Histonet] coverglass In-Reply-To: Message-ID: That's who we use and we don't have any problems, with have the Tissue Tek Glas coverslipper Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Wednesday, January 12, 2011 11:35 AM To: Histonet Subject: [Histonet] coverglass I am looking for vendors for coverglass for an automated coverglasser. I want one where the coverglasses don't stick together. We are currently using Mercedes as our vendor. Any feedback out there for ThermoFisher? -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory P: 585.586.5166 F: 585.586.3137 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Jan 12 13:15:21 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jan 12 13:15:28 2011 Subject: [Histonet] Re: Thyroid smears Message-ID: Cytopreparation of thyroid FNA specimens is basically the cytotechnologists' responsibility. They should have some of the standard textbooks. Air dry for Romanowsky-type (Diff-Quik) staining, of course, and alcohol fix for the Pap stain. The pathologist must decide which of these is to be used. A lot of old-timers like me aren't comfortable with Diff-Quik for cytology. The recent Bethesda System for Reporting Thyroid Cytopathology (Ali SZ and Cibas ES, Springer-Verlag 2010) doesn't get into methods much. It does cite a paper that I would think might be useful if you can get it. Pitman MB at al. Techniques for thyroid FNA. in Diagnostic Cytopathology 2008;36(6):407-424. Bob Richmond Samurai Pathologist Knoxville TN (and snowed in there all this week!) From Wanda.Smith <@t> HCAhealthcare.com Wed Jan 12 13:26:53 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Jan 12 13:26:57 2011 Subject: [Histonet] coverglass In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139CE683FC@NADCWPMSGCMS03.hca.corpad.net> Jennifer, When we previously used a glass coverslipper, we used ThermoFisher's Fisherfinest Superslip coverslips cat #12-545-88. They have some type of glass beads in-between to keep them from sticking together. They actually look dusty, but they are not. They are kind of expensive, due to the treatment, but we very rarely had any stick together. I think we paid about $150 per case which has 10 of the 1 oz boxes in the case. Hope this helps! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Wednesday, January 12, 2011 1:35 PM To: Histonet Subject: [Histonet] coverglass I am looking for vendors for coverglass for an automated coverglasser. I want one where the coverglasses don't stick together. We are currently using Mercedes as our vendor. Any feedback out there for ThermoFisher? -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory P: 585.586.5166 F: 585.586.3137 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Wed Jan 12 13:48:03 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Jan 12 13:48:23 2011 Subject: [Histonet] Job opening Kansas City, Missouri Message-ID: <1841.208.62.167.196.1294861683.squirrel@webmail.realpages.com> Busy derm practice in the process of opening a Dermatopathology laboratory in the next few months and are looking for an experienced Histologist. Candidate must have at least 5 years experience in a working Histology laboratory and be HT or HTL licesnsed. Derm experience preferred but not required. Must be able to perform all routine histology duties solely. Embedding, grossing, accession, coverslipping, microtonomy, record keeping, H&E stain, and special stains to include PAS, GMS, AFB, GRAM. This position will be filled with only one histologist so you must be efficient, able to multitask, problem solve, and have great time management skills. Mohs experience a plus. Would like candidate to be familiar with CLIA and OSHA standards. Applicant should be able to maintain and update all laboratory manuals as needed. Perform all daily, monthly, and yearly Q.A. and Q.C. Salary based on experience. Please email resume to Nicole@dlcjax.com for consideration. From BSullivan <@t> shorememorial.org Wed Jan 12 13:46:44 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Wed Jan 12 13:50:01 2011 Subject: [Histonet] coverglass In-Reply-To: Message-ID: We have an automated coverslipper and use coverslips from Anapath. We purchase them from STAT LAB. The item number is SL-102450. Their phone number is 1-800-442-3573. We have been very pleased with this product and have used them for many years. The price is not too bad either for the case. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Jennifer Campbell To Sent by: Histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] coverglass 01/12/2011 01:35 PM I am looking for vendors for coverglass for an automated coverglasser. I want one where the coverglasses don't stick together. We are currently using Mercedes as our vendor. Any feedback out there for ThermoFisher? -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory P: 585.586.5166 F: 585.586.3137 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Clough <@t> medicine.tamhsc.edu Wed Jan 12 13:51:09 2011 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Wed Jan 12 13:52:18 2011 Subject: [Histonet] Message-ID: Hello everyone! I am trying to set up a histology lab for a university research group and was wondering if anyone would be willing to advise me on what to purchase in the way of disposable blades (low profile vs high), paraffin, cassettes, and anything else that would be needed in order to start embedding sectioning rodent tissue (mainly bone). Any advise would be greatly appreciated! Thanks, Bret From JWeems <@t> sjha.org Wed Jan 12 14:12:15 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jan 12 14:12:21 2011 Subject: [Histonet] coverglass In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139CE683FC@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139CE683FC@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081519E6A9@CHEXCMS10.one.ads.che.org> Cardinal has a good one - must cheaper than that! j M6045-9...http://www.cardinal.com/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Wednesday, January 12, 2011 14:27 To: campbellj@muhlbauerlab.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] coverglass Jennifer, When we previously used a glass coverslipper, we used ThermoFisher's Fisherfinest Superslip coverslips cat #12-545-88. They have some type of glass beads in-between to keep them from sticking together. They actually look dusty, but they are not. They are kind of expensive, due to the treatment, but we very rarely had any stick together. I think we paid about $150 per case which has 10 of the 1 oz boxes in the case. Hope this helps! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Wednesday, January 12, 2011 1:35 PM To: Histonet Subject: [Histonet] coverglass I am looking for vendors for coverglass for an automated coverglasser. I want one where the coverglasses don't stick together. We are currently using Mercedes as our vendor. Any feedback out there for ThermoFisher? -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory P: 585.586.5166 F: 585.586.3137 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ltougas <@t> dawsoncollege.qc.ca Wed Jan 12 14:12:53 2011 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Wed Jan 12 14:12:58 2011 Subject: [Histonet] AO microtome Message-ID: <7618BC6D53F39149B7B57615C218AADF1C9095510A@EXCHANGE.ad.dawsoncollege.qc.ca> Hi everyone, At our College some of the microtomes we teach microtomy with are good old 820 AOs! We have 6 that were impeccably maintained and work very well. However, one of them is very stiff but, again, provides very good 5 um sections. It had been put away but, as a new teacher to this course, I was wondering if any of you would have an idea, other than oiling and greasing, which has already been done, of what could help the handwheel behing less hard to turn. I do not see anyone working on it more than 30 minutes, as it is now! Thank you in advance for any advice! Liette Tougas, Medical Laboratory Department Dawson College, Montreal 514-931-8731, ext 1519 From thisisann <@t> aol.com Wed Jan 12 15:24:04 2011 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Wed Jan 12 15:24:40 2011 Subject: [Histonet] Fetal Choline esterase stain Message-ID: <8CD80B62C8BCDA1-6B8-FB67@webmail-m003.sysops.aol.com> Does anyone have a procedure for fetal choline esterase stain on formalin fixed colonic mucosal tissue for Hirschprung disease? From mpence <@t> grhs.net Wed Jan 12 15:29:12 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jan 12 15:29:17 2011 Subject: [Histonet] Job opening Kansas City, Missouri In-Reply-To: <1841.208.62.167.196.1294861683.squirrel@webmail.realpages.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974AFE@is-e2k3.grhs.net> No vacation! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Wednesday, January 12, 2011 1:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job opening Kansas City, Missouri Busy derm practice in the process of opening a Dermatopathology laboratory in the next few months and are looking for an experienced Histologist. Candidate must have at least 5 years experience in a working Histology laboratory and be HT or HTL licesnsed. Derm experience preferred but not required. Must be able to perform all routine histology duties solely. Embedding, grossing, accession, coverslipping, microtonomy, record keeping, H&E stain, and special stains to include PAS, GMS, AFB, GRAM. This position will be filled with only one histologist so you must be efficient, able to multitask, problem solve, and have great time management skills. Mohs experience a plus. Would like candidate to be familiar with CLIA and OSHA standards. Applicant should be able to maintain and update all laboratory manuals as needed. Perform all daily, monthly, and yearly Q.A. and Q.C. Salary based on experience. Please email resume to Nicole@dlcjax.com for consideration. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jan 12 16:07:14 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jan 12 16:07:18 2011 Subject: [Histonet] CAP #ANP.21382 Reagent Expiration Date. Message-ID: <637484.17822.qm@web113807.mail.gq1.yahoo.com> Hi Everyone in Histoland!? Happy Hump day!? I have 2 questions to ask you.? The CAP "#ANP.21382 Reagent Expiration Date", Evidence of compliance requires a written policy for evaluating reagents lacking manufacturer's expiration date. I worked in the Biotech arena for several years in R&D and manufacturing.? We had procedures which were in compliance to GLP and manufacturing standards.? These policies were much more rigid than for AP departments, who adher to CAP requirements.? Would any of you kind people like to share a copy of your written policy with me.? I would be forever grateful! Second question: How do you address powder dyes, and chemicals?in crystalin and powder form, which are extremely old and do not have an expiration date?? What is your criteria for passing of failing these chemicals and dyes?? Do you visually inspect these chemicals and dyes on an annual basis, and if they look fine, give it another year for a visual check with next years date, or do you send them out to a company for analysis to see if they past required specifications???? That would be pretty costly! If anyone has a written procedure for this I would love to see it too! Thank you in advance for your assistance. Akemi Akemi Allison BS, HT(ASCP)HTL E-Mail: akemiat3377@yahoo.com From eridana <@t> cox.net Wed Jan 12 16:19:54 2011 From: eridana <@t> cox.net (Eridana) Date: Wed Jan 12 16:19:58 2011 Subject: [Histonet] Excellent Low and High vinyl chairs Message-ID: <20110112171954.GLSRY.816437.imail@fed1rmwml44> There was some discussion last week and I dug up the info on my favorite chairs. I purchased some excellent chairs at a very reasonable cost from eStores that sells chairs from Office Master in Ontario, CA. The chairs come in all kinds of fabric including the lab vinyl. The distributor's site is http://www.officemasterchairs.com/budget-task-chairs-c-26.html. I got both the BC46 (low) and the BC41 (high) and they were S117.10 and $122.50 with a 5% discount for 3 or more chairs. They as good as if not better than any other chair I have used in the past 30+ years. Donna Harclerode, HT,HTL (ASCP) QIHC, SLS Immunohistochemist 10455 Pacific Center Court San Diego, CA 92121 858-768-5378 Donna.Harclerode@aristamolecular.com ******************************************************************************************************************* Confidentiality Notice: This electronic mail message may contain information which is (a) LEGALLY PRIVILEGED, CONFIDENTIAL AND PROPRIETARY IN NATURE, OR OTHERWISE PROTECTED BY LAW FROM DISCLOSURE, and which is (b) intended only for the use of the Addressee(s) names herein. If you are not the Addressee(s), or the person responsible for delivering this to the Addressee(s), you are hereby notified that reading, copying, or distributing this message is prohibited. If you have received this electronic message in error, please contact me immediately at the telephone number shown above and take the steps necessary to delete the message completely from your computer system. Thank you. From LSetlak <@t> childrensmemorial.org Wed Jan 12 16:26:46 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Wed Jan 12 16:26:52 2011 Subject: [Histonet] Fetal Choline esterase stain In-Reply-To: <8CD80B62C8BCDA1-6B8-FB67@webmail-m003.sysops.aol.com> References: <8CD80B62C8BCDA1-6B8-FB67@webmail-m003.sysops.aol.com> Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7682E0@CMHEXCC01MBX.childrensmemorial.org> We perform the stain but it is on frozen tissue. Let me know if you woul like the procedure. Lisa lsetlak@childrensmemorial.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com Sent: Wednesday, January 12, 2011 3:24 PM To: histonet@lists.utsouthwestern.edu Cc: ofuks@bioreference.com Subject: [Histonet] Fetal Choline esterase stain Does anyone have a procedure for fetal choline esterase stain on formalin fixed colonic mucosal tissue for Hirschprung disease? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Jan 12 16:43:36 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 12 16:43:56 2011 Subject: [Histonet] Repair company in So. CA Message-ID: Hi All, I have a facility that needs to have repairs done on a VIP 5. They are looking for a company to service this instrument in Southern California, other than Sakura. They are looking for a reliable company with reasonable rates. Thank you, Jennifer MacDonald From laurie.colbert <@t> huntingtonhospital.com Wed Jan 12 17:14:36 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jan 12 17:14:40 2011 Subject: [Histonet] Repair company in So. CA In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D76830A50B0C9@EXCHANGE3.huntingtonhospital.com> I use Mikron for most of my equipment, including my VIP 5. Their number is (800) 377-5395, and they are somewhere in Orange County. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, January 12, 2011 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Repair company in So. CA Hi All, I have a facility that needs to have repairs done on a VIP 5. They are looking for a company to service this instrument in Southern California, other than Sakura. They are looking for a reliable company with reasonable rates. Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMyers1 <@t> aol.com Wed Jan 12 19:55:06 2011 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Wed Jan 12 19:55:15 2011 Subject: [Histonet] Thyroid Smears Message-ID: Fawn: Fixation of exceptionally 'fluid' cytologic specimens, like many thyroid aspirates, through a submersion technique will nearly always result in a significant loss of material from the slides. And less important than the loss of what appears to be blood is the loss of thyroid epithelial cells/tissue fragments. If the amount of blood in the specimen appears, grossly, to be 'excessive', then, as Debbie suggested, adding PlasmaLyte to the fluid prior to smear preparation is a very practical option. Assuming that the smears will be Pap-stained, then the best way to prepare them is to express one or two drops of the aspirate onto a slide, spread the material using another slide, spray-fix both smears with (a commercially-prepared solution containing primarily) ethanol, and then air dry them thoroughly before staining. And if some of the smears will be immuno-stained in a protocol designed/validated for histologic material, these slides may be spray-fixed with 10% formalin instead of ethanol. Good Luck, Joe Myers, M.S., CT(ASCP)QIHC ------------------------------ Message: 9 Date: Wed, 12 Jan 2011 09:33:06 -0500 From: Fawn Bomar Subject: [Histonet] Thyroid Smears To: "histonet@lists.utsouthwestern.edu" Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn From akemiat3377 <@t> yahoo.com Wed Jan 12 20:21:13 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jan 12 20:21:18 2011 Subject: [Histonet] Fw: CLARIFICATION: CAP #ANP.21382 Reagent Expiration Date. Message-ID: <355155.66640.qm@web113817.mail.gq1.yahoo.com> Thanks Laurie, and Histoland, My client does what Laurie suggests. Perhaps, I need to clarify: Please read information below: This is regarding RAW chemicals such as: Iodine crystals, Sodium Thiosulfate, Mercuric Chloride, and Copper, etc, and dry Dye Stains, that do not have expiration dates. I don't have a written policy for evaluation of these chemicals and dyes that do not have an expiration date. How do I know they can use these chemicals and dyes to make up the final end solutions. My client currently visualy inspects these chemicals and dyes to see if they appear to be OK, they put another year to review "visually". How can an untrained, non-chemist, judge by visual inspecition if the chemical is OK??? I need a written policy to adhere to CAP guidelines. Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ________________________________ From: Laurie Colbert To: Akemi Allison Sent: Wed, January 12, 2011 3:17:20 PM Subject: RE: [Histonet] CAP #ANP.21382 Reagent Expiration Date. Hi Akemi, Our procedure for evaluating stains/reagents is to run control tissue. If the control works, the stain/reagent is good. And that is done much more often than annually - it is done every time the stain is performed. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 12, 2011 2:07 PM To: histonet Subject: [Histonet] CAP #ANP.21382 Reagent Expiration Date. Hi Everyone in Histoland! Happy Hump day! I have 2 questions to ask you. The CAP "#ANP.21382 Reagent Expiration Date", Evidence of compliance requires a written policy for evaluating reagents lacking manufacturer's expiration date. I worked in the Biotech arena for several years in R&D and manufacturing. We had procedures which were in compliance to GLP and manufacturing standards. These policies were much more rigid than for AP departments, who adher to CAP requirements. Would any of you kind people like to share a copy of your written policy with me. I would be forever grateful! Second question: How do you address powder dyes, and chemicals in crystalin and powder form, which are extremely old and do not have an expiration date? What is your criteria for passing of failing these chemicals and dyes? Do you visually inspect these chemicals and dyes on an annual basis, and if they look fine, give it another year for a visual check with next years date, or do you send them out to a company for analysis to see if they past required specifications??? That would be pretty costly! If anyone has a written procedure for this I would love to see it too! Thank you in advance for your assistance. Akemi Akemi Allison BS, HT(ASCP)HTL E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ann.bennettann <@t> yahoo.com Thu Jan 13 05:37:04 2011 From: ann.bennettann <@t> yahoo.com (Ann Bennett) Date: Thu Jan 13 05:37:08 2011 Subject: [Histonet] Inconsistent immuno staining Message-ID: <821615.18524.qm@web114316.mail.gq1.yahoo.com> Hello Histonetters, ? While trying to eliminate any variables - processing times, embedding paraffin, waterbath temperature, time and temp in oven, deparaffinization, antigen retrieval, antibody manufacturer and lot # - I still seem to have a slight inconsistency with 1 of my immuno stains. ? Mast Cell Tryptase staining for colon biopsies. ? Can anyone offer any suggestions or ideas as to why this might be occuring? ? Even within my current control slides I see a nice distinct brown or a slightly more pale stain. ? I would love to hear your ideas. ? Thank you! ? Ann.BennettAnn From jaylundgren <@t> gmail.com Thu Jan 13 07:14:37 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Jan 13 07:14:41 2011 Subject: [Histonet] AO microtome In-Reply-To: <7618BC6D53F39149B7B57615C218AADF1C9095510A@EXCHANGE.ad.dawsoncollege.qc.ca> References: <7618BC6D53F39149B7B57615C218AADF1C9095510A@EXCHANGE.ad.dawsoncollege.qc.ca> Message-ID: Bonjour! There is a big screw which holds the fine advance wheel to the body of the microtome. Take a HUGE screwdriver and loosen the screw a bit. Et voila! The AO 820 was my first love....sigh. Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adam.smith <@t> adeccona.com Thu Jan 13 08:25:31 2011 From: adam.smith <@t> adeccona.com (Smith, Adam) Date: Thu Jan 13 08:25:45 2011 Subject: [Histonet] Histology manager needed in Agusta GA Message-ID: <0980CC29978E784588667491783476B80A54FFBE98@MEUSITINFEVS02.am.adecco.net> * Very competitive pay / benefit package * Must have Active/current certification s a Histotechnologist (HT or HTL) by the Board of Registry of the American Society of Clinical Pathologists * Five years experience as an HTIII to include 2 years of management experience or 3-5 years experience as an HTIII Please contact me if you are interested Regards, Adam Smith Medical & Science recruiting specialist Office: 585.454.5511 Toll Free: 866.217.3454 From aonomic <@t> auburn.edu Thu Jan 13 08:34:18 2011 From: aonomic <@t> auburn.edu (Michelle Aono) Date: Thu Jan 13 08:34:39 2011 Subject: [Histonet] Reichert-Jung 2040 Maintenance Message-ID: <4D2EB908.5876.00D9.0@auburn.edu> Our microtome is old and no longer serviced by Leica. Does anyone know if independent contractors can do routine maintenance on old microtomes and how I might find them? Thanks. Shelly ~~~~~~~~~~~~~~~ Michelle (Shelly) Aono Histology Technician IV CVM & AU Staff Advisory Council Representative Auburn University CVM-APP 212 Greene Hall, Auburn, AL 36832 (334) 844-5594 From adam.smith <@t> adeccona.com Thu Jan 13 09:00:38 2011 From: adam.smith <@t> adeccona.com (Smith, Adam) Date: Thu Jan 13 09:00:50 2011 Subject: [Histonet] Opening for a Histology Manager in Augusta GA Message-ID: <0980CC29978E784588667491783476B80A54FFBF2E@MEUSITINFEVS02.am.adecco.net> Hello, I have a job opening for a Histology Manager located in Augusta GA (I apologize that I spelled this incorrectly in my last post). If you are interested, please reach out to me directly. * Very competitive pay / benefit package * Must me located in Augusta or be willing to relocate to Augusta GA * Must have Active/current certification s a Histotechnologist (HT or HTL) by the Board of Registry of the American Society of Clinical Pathologists * Five years experience as an HTIII to include 2 years of management experience or 3-5 years experience as an HTIII Regards, Adam Smith Medical & Science recruiting specialist Office: 585.454.5511 Toll Free: 866.217.3454 From ccrowder <@t> vetmed.lsu.edu Thu Jan 13 09:40:28 2011 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Jan 13 09:43:06 2011 Subject: [Histonet] Dyes and chemicals Message-ID: Akemi = According to the Biological Stain Commission, dye often get better with age. If you can run a control slide with a stain that uses the dye and it is positive, GLP regs allow you to put an expiration date 1 year from that time on the dye. You can keep doing this procedure and redate the dye for a long time. Chemicals, on the other hand, can outdate. Your best bet is to dispose of them properly. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 From JMacDonald <@t> mtsac.edu Thu Jan 13 10:11:22 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jan 13 10:11:27 2011 Subject: [Histonet] Repair company in So. CA In-Reply-To: Message-ID: I would like to clarify my request below. The reason I stated other than Sakura is that they already know of them and were just wondering who else did repairs. It was not that Sakura does not do good work. Sakura does our repairs for us on our VIP and we have been more than satisfied. Jennifer Jennifer MacDonald Sent by: histonet-bounces@lists.utsouthwestern.edu 01/12/2011 02:46 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Repair company in So. CA Hi All, I have a facility that needs to have repairs done on a VIP 5. They are looking for a company to service this instrument in Southern California, other than Sakura. They are looking for a reliable company with reasonable rates. Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Thu Jan 13 11:03:29 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Jan 13 11:03:36 2011 Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA Message-ID: <24A4826E8EF0964D86BC5317306F58A55568F14218@mmc-mail.ad.mhsil.com> WHAT IS EVERYONE USING FOR THE CPT CODES ON RE-EXCISIONS OF MELANOMA FOR MARGINS? IN THE PAST WE HAVE BEEN USING 88307 IF IT WAS A DEEP EXCISION WITH NO RESIDUAL TUMOR AND AN 88309 WHEN IT WAS A DEEP EXCISION WITH RESIDUAL TUMOR. WE HAVE NOW BEEN QUESTIONED ON WHETHER ALL SHOULD ACTUALLY BE 88305. How is everyone else charging these? I guess the next question is what constitutes a deep excision? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 13 11:01:36 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 13 11:05:27 2011 Subject: [Histonet] brachial plexus repair frozen sections Message-ID: Is anyone involved in doing intraoperative frozen sections during brachial plexus repair surgery? If so, would you mind contacting me off-line? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From BSullivan <@t> shorememorial.org Thu Jan 13 11:13:14 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Thu Jan 13 11:16:29 2011 Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55568F14218@mmc-mail.ad.mhsil.com> Message-ID: Jim, To my knowledge the CPT code for melanomas, including re-excision, is 88305. The 88507 can be applied if it is in soft tissue or mets. Skin is usually what we deal with so we bill 88305. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Vickroy, Jim" To Sent by: "histonet@lists.utsouthwestern.edu" histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] CPT CODES FOR 01/13/2011 12:03 RE-EXCISIONS OF MELANOMA PM WHAT IS EVERYONE USING FOR THE CPT CODES ON RE-EXCISIONS OF MELANOMA FOR MARGINS? IN THE PAST WE HAVE BEEN USING 88307 IF IT WAS A DEEP EXCISION WITH NO RESIDUAL TUMOR AND AN 88309 WHEN IT WAS A DEEP EXCISION WITH RESIDUAL TUMOR. WE HAVE NOW BEEN QUESTIONED ON WHETHER ALL SHOULD ACTUALLY BE 88305. How is everyone else charging these? I guess the next question is what constitutes a deep excision? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Jan 13 11:20:49 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 13 11:20:56 2011 Subject: [Histonet] RE: CPT CODES FOR RE-EXCISIONS OF MELANOMA In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55568F14218@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A55568F14218@mmc-mail.ad.mhsil.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081519E863@CHEXCMS10.one.ads.che.org> I think a skin is a skin is a skin. We use 88305. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, January 13, 2011 12:03 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA WHAT IS EVERYONE USING FOR THE CPT CODES ON RE-EXCISIONS OF MELANOMA FOR MARGINS? IN THE PAST WE HAVE BEEN USING 88307 IF IT WAS A DEEP EXCISION WITH NO RESIDUAL TUMOR AND AN 88309 WHEN IT WAS A DEEP EXCISION WITH RESIDUAL TUMOR. WE HAVE NOW BEEN QUESTIONED ON WHETHER ALL SHOULD ACTUALLY BE 88305. How is everyone else charging these? I guess the next question is what constitutes a deep excision? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jerrysedgewick <@t> gmail.com Thu Jan 13 11:35:07 2011 From: jerrysedgewick <@t> gmail.com (Jerry (Gerald) Sedgewick) Date: Thu Jan 13 11:35:18 2011 Subject: [Histonet] new listserv Message-ID: <4D2F37CB.9050603@gmail.com> Hello All, If you or users of your facility could benefit from a free forum on scientific imaging and image analysis, I'm hosting one. This is not a listserv that operates like histonet, but a web forum to give users the option of uploading images for evaluation. For more information and to register, go to: http://www.imagingandanalysis.com/instruction.html Cheers, Jerry -- Jerry Sedgewick Sedgewick Initiatives 965 Cromwell Avenue Saint Paul, MN 55114 651-788-2261 jerrysedgewick@gmail.com http://www.imagingandanalysis.com Author of: "Scientific Imaging with Photoshop: Methods, Measurement, and Output" From mthomas <@t> littonlab.com Thu Jan 13 11:42:46 2011 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Thu Jan 13 11:42:50 2011 Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55568F14218@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A55568F14218@mmc-mail.ad.mhsil.com> Message-ID: <9971590C6DBC446198439BB004EBFB1D@LittonPath.local> We to use 88305 because it is a skin, in the CPT code book there is no option for larger codes because it is a re-excision with margins or melanoma. If you want clarification you can go on the CAP website and get CPT coding help. You can email them with questions and they will respond. Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, January 13, 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA WHAT IS EVERYONE USING FOR THE CPT CODES ON RE-EXCISIONS OF MELANOMA FOR MARGINS? IN THE PAST WE HAVE BEEN USING 88307 IF IT WAS A DEEP EXCISION WITH NO RESIDUAL TUMOR AND AN 88309 WHEN IT WAS A DEEP EXCISION WITH RESIDUAL TUMOR. WE HAVE NOW BEEN QUESTIONED ON WHETHER ALL SHOULD ACTUALLY BE 88305. How is everyone else charging these? I guess the next question is what constitutes a deep excision? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.Whitaker <@t> osumc.edu Thu Jan 13 11:43:58 2011 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Thu Jan 13 11:44:00 2011 Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA Message-ID: <6B106EE8C8AAEF449DEA97921DEC1167015365CCC9@EXMBOX-VP05.OSUMC.EDU> The only time a skin is above an 88305 is when it is just the 'covering' on something that has soft tissue involvement to the point that it then becomes a "soft tissue" specimen, rather than a skin. That's my take on it. Bonnie Whitaker AP Operations Director Ohio State University Medical Center Department of Pathology 614.293.5048 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, January 13, 2011 12:21 PM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CPT CODES FOR RE-EXCISIONS OF MELANOMA I think a skin is a skin is a skin. We use 88305. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, January 13, 2011 12:03 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA WHAT IS EVERYONE USING FOR THE CPT CODES ON RE-EXCISIONS OF MELANOMA FOR MARGINS? IN THE PAST WE HAVE BEEN USING 88307 IF IT WAS A DEEP EXCISION WITH NO RESIDUAL TUMOR AND AN 88309 WHEN IT WAS A DEEP EXCISION WITH RESIDUAL TUMOR. WE HAVE NOW BEEN QUESTIONED ON WHETHER ALL SHOULD ACTUALLY BE 88305. How is everyone else charging these? I guess the next question is what constitutes a deep excision? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Jan 13 11:57:55 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 13 11:58:00 2011 Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA In-Reply-To: <9971590C6DBC446198439BB004EBFB1D@LittonPath.local> References: <24A4826E8EF0964D86BC5317306F58A55568F14218@mmc-mail.ad.mhsil.com> <9971590C6DBC446198439BB004EBFB1D@LittonPath.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081519E89A@CHEXCMS10.one.ads.che.org> And if you find something different, please let us know! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marla Thomas Sent: Thursday, January 13, 2011 12:43 To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA We to use 88305 because it is a skin, in the CPT code book there is no option for larger codes because it is a re-excision with margins or melanoma. If you want clarification you can go on the CAP website and get CPT coding help. You can email them with questions and they will respond. Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, January 13, 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA WHAT IS EVERYONE USING FOR THE CPT CODES ON RE-EXCISIONS OF MELANOMA FOR MARGINS? IN THE PAST WE HAVE BEEN USING 88307 IF IT WAS A DEEP EXCISION WITH NO RESIDUAL TUMOR AND AN 88309 WHEN IT WAS A DEEP EXCISION WITH RESIDUAL TUMOR. WE HAVE NOW BEEN QUESTIONED ON WHETHER ALL SHOULD ACTUALLY BE 88305. How is everyone else charging these? I guess the next question is what constitutes a deep excision? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Mark.Elliott <@t> hli.ubc.ca Thu Jan 13 12:01:35 2011 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Thu Jan 13 12:02:33 2011 Subject: [Histonet] Re: Histonet Digest, Vol 86, Issue 17 In-Reply-To: <1847E2D4.591@mail.mrl.ubc.ca> References: <1847E2D4.591@mail.mrl.ubc.ca> Message-ID: <4D2ECD7F020000D60005A204@mail.mrl.ubc.ca> Thanks Mike I have passed it on to the person who was asking. Mark Message: 1 Date: Wed, 12 Jan 2011 13:02:35 -0500 From: mtitford@aol.com Subject: [Histonet] Deparaffinization for EM To: histonet@lists.utsouthwestern.edu Message-ID: <8CD809A06C3774E-13C4-538@webmail-d023.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Mark Elliot asks about processing FFPE tissue for EM. Hayat published a method in his book we use. Briefly you dig out 1mm cube pieces of tissue from the block and rotate them in xylene for one hour, followed by absolute, 95%, 70%,50% alcohols for 15 minutes each and then into your EM buffer overnight, followed by your normal EM processing next day. The EM image looks pretty grotty but if your pathologist can make a diagnosis it is worth it. (Hayat M.A. Principles and techniques of electron microscopy. 2ed edition. Baltimore. University Park Press. 1981 page 209) In another method published years ago, someone by-passed the hydration steps by deparaffizing in osmium dissolved in xylene, then going straight to Epon. Hope this helps Michael Titford Pathology USA Mobile AL USA ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From mtitford <@t> aol.com Thu Jan 13 12:24:34 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Jan 13 12:24:55 2011 Subject: [Histonet] Hirschsprungs disease? Try Calretinin! Message-ID: <8CD816643211E49-13B4-E221@webmail-d072.sysops.aol.com> Someone who did not give their name enquired about "Fetal choline esterase" for Hirschsprungs disease on formalin fixed tissue.(That word was probably "Acetyl). We used to use the old acetyl thiocholine esterase on frozen sections but switched a couple of years ago to calretinin histochemistry on routine FFPE sections. It works! We also do H&E levels as well. The reference is: Guinard-Samuel, V: Borrard, Arnaud:De Lagausie, Pascual et al. "Calretinin immunohistochemistry: a simple and efficient tool to diagnose Hirschsprungs disease" Modern Pathology 22: 1379-1384. 2009 Hope this help Michael Titford Pathology USA Mobile AL USA From AnthonyH <@t> chw.edu.au Thu Jan 13 15:51:55 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jan 13 15:52:08 2011 Subject: [Histonet] Inconsistent immuno staining In-Reply-To: <821615.18524.qm@web114316.mail.gq1.yahoo.com> References: <821615.18524.qm@web114316.mail.gq1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A715705E63D@xmdb02.nch.kids> Ann, I assume you are demonstrating mast cells. Mast cells are very small cells and on sectioning it is easy to only have less than half a mast cell in your sections, where as you will find whole mast cells close by. This difference will mirror your staining: strong staining in the whole cells, weaker staining in the sectioned cells. You will see the same occurring with the mast cell metachromatic stains (toluidine blue or Giemsa), strong and weaker staining depending on whether you have whole or sectioned cells. Does this help? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Bennett Sent: Thursday, 13 January 2011 10:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inconsistent immuno staining Hello Histonetters, ? While trying to eliminate any variables - processing times, embedding paraffin, waterbath temperature, time and temp in oven, deparaffinization, antigen retrieval, antibody manufacturer and lot # - I still seem to have a slight inconsistency with 1 of my immuno stains. ? Mast Cell Tryptase staining for colon biopsies. ? Can anyone offer any suggestions or ideas as to why this might be occuring? ? Even within my current control slides I see a nice distinct brown or a slightly more pale stain. ? I would love to hear your ideas. ? Thank you! ? Ann.BennettAnn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Reuel.Cornelia <@t> tsrh.org Thu Jan 13 16:15:49 2011 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Jan 13 16:16:17 2011 Subject: [Histonet] Cryojane Model 400 Message-ID: <4D2F2535.B80A.00C5.1@tsrh.org> I just want to know if anybody out there in histoland have an Instrumedics cryojane Model 400 that is more than 10 years old and the spare parts like the UV bulb, heating pad are still supported by a company. Leica just told me this afternoon that they do not support any spare parts of an instrument more than 10 years old. Please give me your feedback. Thank you. Reuel From TJJ <@t> stowers.org Fri Jan 14 10:31:10 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Jan 14 10:31:18 2011 Subject: [Histonet] LR White embedding for Immuno EM Message-ID: Posted for a colleague of mine: I am currently trying to get a GFP antibody to work on mouse intestinal crypts. I am using LR White embedded sections that were fixed in 4% PFA/0.1% GA in PBS. I put them in a flat embedding mold and cured in the oven at 60 C. Most of the LR White evaporated out overnight so I had to re-embed the sample. The paneth cells look as if they did not infiltrate well after re-embedding in gelatin capsules and I lost orientation. Is there any way to recover this sample or will I need to start over again with new tissue sample? Rhonda Trimble HT(ASCP)HTL, QIHC Electron Microscopy Specialist Stowers Institute for Medical Research 1000 E 50th Street Kansas City, Missouri 64110 816-926-4346 rra@stowers.org From nicholer <@t> slonepartners.com Fri Jan 14 12:01:49 2011 From: nicholer <@t> slonepartners.com (Nichole Ramis) Date: Fri Jan 14 12:02:01 2011 Subject: [Histonet] Histotechnologist position in DC Message-ID: > Slone Partners seeks a Histotechnologist for a cutting edge hospital > laboratory, based in a beautiful Washington DC neighborhood. > > ASCP certification is preferred with at least 2 years of experience in a > high-volume laboratory. > > Special features of the position: This organization embraces curiosity and > enjoys mentoring and developing its employees. > > Interested and qualified candidates should submit their resume to Kim Wilson > at kimd@slonepartners.com. From smcbride <@t> andrew.cmu.edu Fri Jan 14 13:55:55 2011 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Fri Jan 14 13:55:59 2011 Subject: [Histonet] Request for Coverslip Removal SOP Message-ID: <885E561F-0015-4A57-8875-D0EB5F6E3890@andrew.cmu.edu> Hi colleagues, I need to remove a glass coverslip from a mounted H&E biopsy slide with an implant in order to run some biomaterial surface characterization studies. Does anyone have a SOP that they would be willing to share for removing glass coverslips without damaging the specimen? Thanks in advance for all of the great advice that I always get from the histonet. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu From NMargaryan <@t> childrensmemorial.org Fri Jan 14 13:54:02 2011 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Jan 14 13:56:54 2011 Subject: [Histonet] Antigen Retrieval for 10u sections in IHC Message-ID: Hi tistonetters, I have to do IHC on 10? sections. Is procedure for Antigen Retrieval same like for 4-5? (time and temperature) ???????? Thanks in advance, Naira From foreightl <@t> gmail.com Fri Jan 14 14:08:39 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Jan 14 14:12:41 2011 Subject: [Histonet] Request for Coverslip Removal SOP In-Reply-To: <885E561F-0015-4A57-8875-D0EB5F6E3890@andrew.cmu.edu> References: <885E561F-0015-4A57-8875-D0EB5F6E3890@andrew.cmu.edu> Message-ID: soaking in xylene for at least a couple of hours (overnight is best) works for us. On Fri, Jan 14, 2011 at 11:55 AM, Sean McBride wrote: > Hi colleagues, > > I need to remove a glass coverslip from a mounted H&E biopsy slide with an > implant in order to run some biomaterial surface characterization studies. > Does anyone have a SOP that they would be willing to share for removing > glass coverslips without damaging the specimen? Thanks in advance for all > of the great advice that I always get from the histonet. > > > Best regards, > > > ~Sean McBride > > > Scientific Specialist > Bone Tissue Engineering Center > Carnegie Mellon Research Institute > Suite 4311 > 700 Technology Drive > Pittsburgh, PA 15219-3124 > > 412-268-8275 (o) > 412-915-1683 (m) > 412-268-8275 (fax) > smcbride@andrew.cmu.edu > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From LSetlak <@t> childrensmemorial.org Fri Jan 14 14:15:21 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Fri Jan 14 14:15:50 2011 Subject: [Histonet] Request for Coverslip Removal SOP In-Reply-To: <885E561F-0015-4A57-8875-D0EB5F6E3890@andrew.cmu.edu> References: <885E561F-0015-4A57-8875-D0EB5F6E3890@andrew.cmu.edu> Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA768308@CMHEXCC01MBX.childrensmemorial.org> Hi, I don't have an actual SOP but we just soak in a jar of xylene until the coverslip come off. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Friday, January 14, 2011 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Request for Coverslip Removal SOP Importance: High Hi colleagues, I need to remove a glass coverslip from a mounted H&E biopsy slide with an implant in order to run some biomaterial surface characterization studies. Does anyone have a SOP that they would be willing to share for removing glass coverslips without damaging the specimen? Thanks in advance for all of the great advice that I always get from the histonet. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PowersK <@t> ccmhonline.com Fri Jan 14 15:31:50 2011 From: PowersK <@t> ccmhonline.com (Powers, Kerry) Date: Fri Jan 14 15:33:47 2011 Subject: [Histonet] decalcifying bone marrows after processing Message-ID: <1CC65327E394154384235C226256AD8013C74C@ccmhintra.com> I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 From rjbuesa <@t> yahoo.com Fri Jan 14 15:42:21 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 14 15:42:23 2011 Subject: [Histonet] decalcifying bone marrows after processing In-Reply-To: <1CC65327E394154384235C226256AD8013C74C@ccmhintra.com> Message-ID: <382418.24220.qm@web65712.mail.ac4.yahoo.com> If you what to do a histology work of quality, you cannot decalcify after processing, besides, what is the point? It is preferable to use formic acid (even if?it is worse than using EDTA) than having to struggle with a poor section produced?and then?trying to decalcify it. This is typical of the ignorance of most pathologists?about?tissue processing?things. Ren? J. --- On Fri, 1/14/11, Powers, Kerry wrote: From: Powers, Kerry Subject: [Histonet] decalcifying bone marrows after processing To: histonet@lists.utsouthwestern.edu Date: Friday, January 14, 2011, 4:31 PM I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed.? Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification.? She asked if we could process them and then decalcify and I have yet to find an answer to this question.? Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Jan 14 15:54:55 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jan 14 15:55:23 2011 Subject: [Histonet] decalcifying bone marrows after processing In-Reply-To: <382418.24220.qm@web65712.mail.ac4.yahoo.com> Message-ID: Kerry Do you have an old dip and dunk tissue processor sitting around? If so you can set up a program to decal and then process. It's funny when I first read your question I was like why can't they do it in a day. We used to all the time, but that was years ago (late 80's) and we fixed in B-5 which took only two hours. For bone marrow samples even if you are using a zinc formalin or something similar you are going to need to fix for at least 4 to 6 hours prior to decalcification. We used 20% formic acid for about 2 hours and that worked well, we then processed on an old dip and dunk processor with the rest of our small biopsy samples I think it was around 20 to 30 minutes per station and they came out beautiful. You could also try one of those fixative/decal combinations. I don't use them personally but I have worked with samples that have used those reagents and to my surprise they actually came out with decent morphology. Bottom line, I think there are some options out there for you rather than not decaling prior to processing. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 14, 2011 2:42 PM To: histonet@lists.utsouthwestern.edu; KerryPowers Subject: Re: [Histonet] decalcifying bone marrows after processing If you what to do a histology work of quality, you cannot decalcify after processing, besides, what is the point? It is preferable to use formic acid (even if?it is worse than using EDTA) than having to struggle with a poor section produced?and then?trying to decalcify it. This is typical of the ignorance of most pathologists?about?tissue processing?things. Ren? J. --- On Fri, 1/14/11, Powers, Kerry wrote: From: Powers, Kerry Subject: [Histonet] decalcifying bone marrows after processing To: histonet@lists.utsouthwestern.edu Date: Friday, January 14, 2011, 4:31 PM I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed.? Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification.? She asked if we could process them and then decalcify and I have yet to find an answer to this question.? Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Jan 14 15:58:58 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jan 14 15:59:08 2011 Subject: [Histonet] paraffin carry-over problem In-Reply-To: References: Message-ID: <4EBFF65383B74D49995298C4976D1D5E07A8E52E@LSRIEXCH1.lsmaster.lifespan.org> I have never used Clear Rite or any other xylene substitute in my lab. Part of the reason may involve something about teaching an old dog new tricks. But there is another reason too. A few years ago I had to paraffin embed and section tissues containing fibers of a new polymer that was to be used in an artificial kidney device. Xylene severely damaged the polymer. So, at that time I did a bit of research on clearing agents in an attempt to find one that could process these particular specimens without destroying the polymer fibers. I did comparative tests with xylene, toluene, chloroform, tetrahydrofuran, dioxane, and seven different commercial "xylene substitutes". Since I would have to use this solvent not only for processing the tissue, but also for deparaffinizing the slides, I compared the various solvents for their efficiency in dissolving paraffin. I put 20 ml of each solvent in a glass vial, added 10 pellets of the embedding paraffin we use here, placed them on a rotator to swirl them gently, then timed how long it took for the 10 pellets to disappear. What I discovered surprised me. All the "xylene substitutes" took a lot longer than xylene to dissolve the paraffin, in some cases 5 or 6 times as long. I did find a product that worked beautifully for this particular project - "Safeclear" - but I have been reluctant to replace xylene with any of these "xylene substitutes" based on the results of those tests, even though I know a lot of people use them and are apparently satisfied with their results. From sgoebel <@t> mirnarx.com Fri Jan 14 16:08:29 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Jan 14 16:08:34 2011 Subject: [Histonet] decalcifying bone marrows after processing In-Reply-To: References: <382418.24220.qm@web65712.mail.ac4.yahoo.com> Message-ID: So just read the below response. I have used formical in the past which is a fixative and decal at the same time. It works well and the morphology really isn't compromised with this solution. It is made by Decal (that is the company name). Unfortunately even with small bones (I have only used it with mouse sternum and mouse femur) it still is an overnight process. You could try and do it the same day, but I think you will end up screaming and pulling out your hair trying to cut the blocks. In the hospital setting we could get bone marrows out early the next morning. We would fix them in AZF for at least 3 or 4 hours and then decal the biopsy for another hour or two. The clot would sometimes come out before the actual biopsy, but this seemed to give the pathologists at least something to work with if the doctor called them for results. Decal after processing...not a great idea. There is a softener solution you can get from Mastertech called "Easy Cut". This works pretty well when you have a sample that is hard to cut. There is my 2 cents =) Happy Friday Histology Land! Hope everyone has a great long weekend!!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, January 14, 2011 3:55 PM To: Rene J Buesa; histonet@lists.utsouthwestern.edu; KerryPowers Subject: RE: [Histonet] decalcifying bone marrows after processing Kerry Do you have an old dip and dunk tissue processor sitting around? If so you can set up a program to decal and then process. It's funny when I first read your question I was like why can't they do it in a day. We used to all the time, but that was years ago (late 80's) and we fixed in B-5 which took only two hours. For bone marrow samples even if you are using a zinc formalin or something similar you are going to need to fix for at least 4 to 6 hours prior to decalcification. We used 20% formic acid for about 2 hours and that worked well, we then processed on an old dip and dunk processor with the rest of our small biopsy samples I think it was around 20 to 30 minutes per station and they came out beautiful. You could also try one of those fixative/decal combinations. I don't use them personally but I have worked with samples that have used those reagents and to my surprise they actually came out with decent morphology. Bottom line, I think there are some options out there for you rather than not decaling prior to processing. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 14, 2011 2:42 PM To: histonet@lists.utsouthwestern.edu; KerryPowers Subject: Re: [Histonet] decalcifying bone marrows after processing If you what to do a histology work of quality, you cannot decalcify after processing, besides, what is the point? It is preferable to use formic acid (even if?it is worse than using EDTA) than having to struggle with a poor section produced?and then?trying to decalcify it. This is typical of the ignorance of most pathologists?about?tissue processing?things. Ren? J. --- On Fri, 1/14/11, Powers, Kerry wrote: From: Powers, Kerry Subject: [Histonet] decalcifying bone marrows after processing To: histonet@lists.utsouthwestern.edu Date: Friday, January 14, 2011, 4:31 PM I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed.? Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification.? She asked if we could process them and then decalcify and I have yet to find an answer to this question.? Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Fri Jan 14 16:31:14 2011 From: bill501 <@t> mindspring.com (Bill B.) Date: Fri Jan 14 16:31:40 2011 Subject: [Histonet] We need a cryostat Message-ID: I have not looked for a new cryostat in several decades, but need to decide on something before next Wednesday. It will be for low volume, primarily breast sentinel nodes, a few per week. Our equipment dealer only carries one: a Sakura Tissue-Tek Cryo3. I suspect this would be adequate, if not overkill. I wondered if anyone was familiar with this cryostat, had any comments or suggestions for other machines. Thanks, Bill From becky.garrison <@t> jax.ufl.edu Fri Jan 14 16:41:03 2011 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Fri Jan 14 16:41:09 2011 Subject: [Histonet] RE: decalcifying bone marrows after processing In-Reply-To: <1CC65327E394154384235C226256AD8013C74C@ccmhintra.com> References: <1CC65327E394154384235C226256AD8013C74C@ccmhintra.com> Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F2B7C4@JX1B-MAIL1.umc.ufl.edu> We process bone marrow biopsies the same day received. We use a combination decal/ fixative solution (Cal Rite decal) with good results. We keep in this solution a minimum of 3 hours before moving to tissue processor where first 2 solutions are a total of 3 hours in formalin. Although most bone marrow biopsies are received by 2:00 - 3:00pm; we've had some received as late as 4:00pm with good results. Our clinicians place the bone marrow core in pre-filled formalin at the point of collection, also. The Cal Rite is a combination of formaldehyde, formic acid and methanol. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry Sent: Friday, January 14, 2011 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcifying bone marrows after processing I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Sat Jan 15 09:36:31 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Sat Jan 15 09:36:44 2011 Subject: [Histonet] Great Ohio HT Opprotunity Message-ID: <6F33D8418806044682A391273399860F069945DA@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren ,Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamargasto.com. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From bce_services <@t> bellsouth.net Sat Jan 15 12:21:25 2011 From: bce_services <@t> bellsouth.net (bce_services@bellsouth.net) Date: Sat Jan 15 12:21:29 2011 Subject: [Histonet] Cryostat CM 3050 Message-ID: <577281.16884.qm@web83913.mail.sp1.yahoo.com> Hi all, ?I'm looking for an old Leica CM3050 cryostat?if anyone?has a broken or mothballed one they want to sell for parts, Thanking you in advance Brian B A Cornett-Earley BCE Services,LLC Web: bceservicesllc.com Tel 770-329-6370 From mjwilliams <@t> dragonlabusa.com Sat Jan 15 13:04:23 2011 From: mjwilliams <@t> dragonlabusa.com (Michael Williams) Date: Sat Jan 15 13:04:31 2011 Subject: [Histonet] We need a cryostat In-Reply-To: References: Message-ID: <006e01cbb4e7$06bac6a0$143053e0$@com> We are a distributor for Bright Instruments UK - manufacturer of cryostats. Michael Williams mjwilliams@scilogex.com SCILOGEX, LLC Berlin, CT www.SCILOGEX.com I have not looked for a new cryostat in several decades, but need to decide on something before next Wednesday. It will be for low volume, primarily breast sentinel nodes, a few per week. Our equipment dealer only carries one: a Sakura Tissue-Tek Cryo3. I suspect this would be adequate, if not overkill. I wondered if anyone was familiar with this cryostat, had any comments or suggestions for other machines. Thanks, Bill From rcartun <@t> harthosp.org Sat Jan 15 18:16:14 2011 From: rcartun <@t> harthosp.org (Richard Cartun) Date: Sat Jan 15 18:16:25 2011 Subject: [Histonet] RE: decalcifying bone marrows after processing Message-ID: <4D31F2800200007700020A91@gwmail1.harthosp.org> Hi Becky: How long is the specimen in formalin before you put it in Cal Rite decal? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Garrison, Becky" 01/14/11 5:44 PM >>> We process bone marrow biopsies the same day received. We use a combination decal/ fixative solution (Cal Rite decal) with good results. We keep in this solution a minimum of 3 hours before moving to tissue processor where first 2 solutions are a total of 3 hours in formalin. Although most bone marrow biopsies are received by 2:00 - 3:00pm; we've had some received as late as 4:00pm with good results. Our clinicians place the bone marrow core in pre-filled formalin at the point of collection, also. The Cal Rite is a combination of formaldehyde, formic acid and methanol. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry Sent: Friday, January 14, 2011 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcifying bone marrows after processing I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmorales <@t> adrian.edu Sat Jan 15 18:46:12 2011 From: mmorales <@t> adrian.edu (Marti Morales) Date: Sat Jan 15 18:46:16 2011 Subject: [Histonet] Leica 2030 digital counter Message-ID: <4D323FD4.4080906@adrian.edu> Hello all: Does anyone know how to fix a microtome digital counter? My counter on my microtome keeps increasing and displaying random numbers upon each microtome cut. Does this mean I have to change out a battery or that something is in need of maintenance? Any suggestions? Thanks for your help in advance. Kind regards, Marti Morales-Ensign From histotech <@t> imagesbyhopper.com Sun Jan 16 06:44:38 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun Jan 16 06:45:42 2011 Subject: [Histonet] decalcifying bone marrows after processing In-Reply-To: References: <382418.24220.qm@web65712.mail.ac4.yahoo.com> Message-ID: <39D40F8B-961D-4F8F-A5B9-0AB92F69C010@imagesbyhopper.com> We process our bone marrow biopsies the same day we receive them. Specimens are received in formalin for the core and B+ fixative for the clot. The core is placed in decal solution for a fairly short time (30 minutes or so) and then routinely processed. As a routine, at the microtome, core biopsies are faced and soaked by floating on the hot water bath for about 20-30 seconds before being placed on ice and sectioned. Our sections turn out quite nice! As always, your mileage may vary! ;o) Michelle On Jan 14, 2011, at 5:08 PM, wrote: > So just read the below response. I have used formical in the past which is a fixative and decal at the same time. It works well and the morphology really isn't compromised with this solution. It is made by Decal (that is the company name). Unfortunately even with small bones (I have only used it with mouse sternum and mouse femur) it still is an overnight process. You could try and do it the same day, but I think you will end up screaming and pulling out your hair trying to cut the blocks. In the hospital setting we could get bone marrows out early the next morning. We would fix them in AZF for at least 3 or 4 hours and then decal the biopsy for another hour or two. The clot would sometimes come out before the actual biopsy, but this seemed to give the pathologists at least something to work with if the doctor called them for results. Decal after processing...not a great idea. There is a softener solution you can get from Mastertech called "Easy Cut". This works pretty well when you have a sample that is hard to cut. There is my 2 cents =) > Happy Friday Histology Land! > Hope everyone has a great long weekend!!! > > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala > Sent: Friday, January 14, 2011 3:55 PM > To: Rene J Buesa; histonet@lists.utsouthwestern.edu; KerryPowers > Subject: RE: [Histonet] decalcifying bone marrows after processing > > Kerry > > Do you have an old dip and dunk tissue processor sitting around? If so you can set up a program to decal and then process. It's funny when I first read your question I was like why can't they do it in a day. We used to all the time, but that was years ago (late 80's) and we fixed in B-5 which took only two hours. For bone marrow samples even if you are using a zinc formalin or something similar you are going to need to fix for at least 4 to 6 hours prior to decalcification. We used 20% formic acid for about 2 hours and that worked well, we then processed on an old dip and dunk processor with the rest of our small biopsy samples I think it was around 20 to 30 minutes per station and they came out beautiful. You could also try one of those fixative/decal combinations. I don't use them personally but I have worked with samples that have used those reagents and to my surprise they actually came out with decent morphology. Bottom line, I think there are some options out there for you rather than not decaling prior to processing. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, Colorado 80308 > office (303) 682-3949 > fax (303) 682-9060 > www.premierlab.com > > > Ship to Address: > 1567 Skyway Drive, Unit E > Longmont, Colorado 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Friday, January 14, 2011 2:42 PM > To: histonet@lists.utsouthwestern.edu; KerryPowers > Subject: Re: [Histonet] decalcifying bone marrows after processing > > If you what to do a histology work of quality, you cannot decalcify after processing, besides, what is the point? > It is preferable to use formic acid (even if it is worse than using EDTA) than having to struggle with a poor section produced and then trying to decalcify it. > This is typical of the ignorance of most pathologists about tissue processing things. > Ren? J. > > > --- On Fri, 1/14/11, Powers, Kerry wrote: > > > From: Powers, Kerry > Subject: [Histonet] decalcifying bone marrows after processing > To: histonet@lists.utsouthwestern.edu > Date: Friday, January 14, 2011, 4:31 PM > > > I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! > > Thank you, > > Kerry Powers > Comanche Country Memorial Hospital > Department of Pathology > 3401 W Gore, Lawton OK 73505 > (580) 355-8699 ext. 3359 > Fax: (580) 585-5462 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotech <@t> imagesbyhopper.com Sun Jan 16 10:52:29 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun Jan 16 10:52:50 2011 Subject: [Histonet] Request for Coverslip Removal SOP In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC0CFA768308@CMHEXCC01MBX.childrensmemorial.org> References: <885E561F-0015-4A57-8875-D0EB5F6E3890@andrew.cmu.edu> <7111DB39D045004C9CF29E79C71B28BC0CFA768308@CMHEXCC01MBX.childrensmemorial.org> Message-ID: <001101cbb59d$c6316550$52942ff0$@imagesbyhopper.com> We put xylene in a jar, add the slide, cover the jar loosely and put it in a warming oven. Alternately, you could put the jar in a water bath. The warm xylene helps to soften the old mounting media. This could take some time though . old mounting media can be some pretty tough stuff! ;o) Good luck! Michelle From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Friday, January 14, 2011 3:15 PM To: 'Sean McBride'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Request for Coverslip Removal SOP Hi, I don't have an actual SOP but we just soak in a jar of xylene until the coverslip come off. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Friday, January 14, 2011 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Request for Coverslip Removal SOP Importance: High Hi colleagues, I need to remove a glass coverslip from a mounted H&E biopsy slide with an implant in order to run some biomaterial surface characterization studies. Does anyone have a SOP that they would be willing to share for removing glass coverslips without damaging the specimen? Thanks in advance for all of the great advice that I always get from the histonet. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1191 / Virus Database: 1435/3378 - Release Date: 01/13/11 From becky.garrison <@t> jax.ufl.edu Sun Jan 16 14:06:41 2011 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Sun Jan 16 14:06:47 2011 Subject: [Histonet] RE: decalcifying bone marrows after processing In-Reply-To: <4D31F2800200007700020A91@gwmail1.harthosp.org> References: <4D31F2800200007700020A91@gwmail1.harthosp.org> Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F2B8B6@JX1B-MAIL1.umc.ufl.edu> Usually one hour. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager ________________________________ From: Richard Cartun [mailto:rcartun@harthosp.org] Sent: Saturday, January 15, 2011 7:16 PM To: PowersK@ccmhonline.com; Garrison, Becky; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: decalcifying bone marrows after processing Hi Becky: How long is the specimen in formalin before you put it in Cal Rite decal? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Garrison, Becky" 01/14/11 5:44 PM >>> We process bone marrow biopsies the same day received. We use a combination decal/ fixative solution (Cal Rite decal) with good results. We keep in this solution a minimum of 3 hours before moving to tissue processor where first 2 solutions are a total of 3 hours in formalin. Although most bone marrow biopsies are received by 2:00 - 3:00pm; we've had some received as late as 4:00pm with good results. Our clinicians place the bone marrow core in pre-filled formalin at the point of collection, also. The Cal Rite is a combination of formaldehyde, formic acid and methanol. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry Sent: Friday, January 14, 2011 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcifying bone marrows after processing I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MariAnn.Mailhiot <@t> leica-microsystems.com Sun Jan 16 16:04:21 2011 From: MariAnn.Mailhiot <@t> leica-microsystems.com (MariAnn.Mailhiot@leica-microsystems.com) Date: Sun Jan 16 16:04:28 2011 Subject: [Histonet] Leica 2030 digital counter In-Reply-To: <4D323FD4.4080906@adrian.edu> Message-ID: Hi Marti I have some bad news, the digital counter can't be fixed. Leica has also stopped making any parts for the RM2000 family. The RM 2030 microtome was a excellent microtome to use in histology lab. Unfortunately is has reached the end of its life. Reichert did start making the RM2000 family of microtomes in the early nineties. The RM2030 and the Leitz 1512 where my most favorite of the older groups of microtomes. Alas, I must move on to the new and improved versions of microtomes. Kind Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7841 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Marti Morales To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Leica 2030 digital counter 01/15/2011 06:46 PM Hello all: Does anyone know how to fix a microtome digital counter? My counter on my microtome keeps increasing and displaying random numbers upon each microtome cut. Does this mean I have to change out a battery or that something is in need of maintenance? Any suggestions? Thanks for your help in advance. Kind regards, Marti Morales-Ensign _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From mr_dammie <@t> hotmail.com Mon Jan 17 00:58:18 2011 From: mr_dammie <@t> hotmail.com (Dammie MR_Dammie) Date: Mon Jan 17 00:58:25 2011 Subject: [Histonet] Leica BOND 3 slides Message-ID: Hello, At this moment we are demoing a Leica BOND 3 immunostainer. We love the stainer in some ways but we are have one problem. When we look at the slides we see some dirt. There is a lot of dirt at the slide edges, this way we have DAB dirt on some parts of our tissue which is a problem. My questions are: Are you familiar with this problem?Did you get rid of it?And how did you get rid of it? Thank you for your help! Best Regards, Kevin From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jan 17 09:51:24 2011 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jan 17 09:51:28 2011 Subject: [Histonet] Whole mount histology Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43FD0CF2993F@HPEMX3.HealthPartners.int> I am totally unfamiliar regarding whole mount histology as it pertains to prostate histology. Can anyone in histoland assist me in finding information out regarding more specifics, especially in lieu of what costs would be incurred to implement this in a routine hospital histology department. Appreciate any and all guidance!! Dorothy Webb, HT Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From rjbuesa <@t> yahoo.com Mon Jan 17 11:18:56 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 17 11:18:59 2011 Subject: [Histonet] Whole mount histology In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43FD0CF2993F@HPEMX3.HealthPartners.int> Message-ID: <527388.33906.qm@web65709.mail.ac4.yahoo.com> Dorothy: First of all you have to be sure that the micrtotome you are going to use has enough cutting path to cover the embedded specimen. You will also need the so called "mega cassettes" and "mega molds", unless you want to make them yourself out of plastic containers (for the cassettes) and of heavy aluminum foil (for the molds) as I first did in 1990 when I started the procedure at my laboratory. Instead of the molds you could use Leukhard's rectangles. Finally you have to develop a fixation and processing protocol suitable for your specimens. Ren? J. --- On Mon, 1/17/11, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Whole mount histology To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, January 17, 2011, 10:51 AM I am totally unfamiliar regarding whole mount histology as it pertains to prostate histology.? Can anyone in histoland assist me in finding information out regarding more specifics, especially in lieu of what costs would be incurred to implement this in a routine hospital histology department. Appreciate any and all guidance!! Dorothy Webb, HT Regions Histology Technical Specialist? 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Mon Jan 17 11:59:32 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Mon Jan 17 11:59:39 2011 Subject: [Histonet] Whole mount histology Message-ID: Hi Dorothy, I concur with what Rene has mentioned. Main cost would be microtome-related, if you don't have microtomes that can handle mega cassettes. Other process-related costs i.e. fixation, tech time, supplies wouldn't be much different once process was optimized. In fact, tech time is likely reduced, as it may be quicker (depending on the tech) to cut 4-6 mega blocks than 18-24 regular blocks. You'll also have to stain and coverslip the slides creatively, as they may not fit on your stainer. Our pathologists did many cases, but eventually went with regular cassettes and processing. It's a nice procedure to have available. Email me if you need more information i.e. specifics on supplies/process. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From MLunetta <@t> luhcares.org Mon Jan 17 11:59:59 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Mon Jan 17 12:00:23 2011 Subject: [Histonet] decalcifying bone marrows after processing Message-ID: <4D34212F020000A800054224@ns.luhcares.org> We have excellent sucess in decalcifying the bone prior to processing. We make sure the core has been in fixative for 2 hrs prior to the 20 mins in the decal solution (DeCal STAT). It is then rinced in water and placed in line for standard processing. Matt Lunetta HT (ASCP) Longmont United Hospital Message: 3 Date: Sat, 15 Jan 2011 19:16:14 -0500 From: "Richard Cartun" Subject: Re: [Histonet] RE: decalcifying bone marrows after processing To: ,, Message-ID: <4D31F2800200007700020A91@gwmail1.harthosp.org> Content-Type: text/plain; charset=US-ASCII Hi Becky: How long is the specimen in formalin before you put it in Cal Rite decal? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Garrison, Becky" 01/14/11 5:44 PM >>> We process bone marrow biopsies the same day received. We use a combination decal/ fixative solution (Cal Rite decal) with good results. We keep in this solution a minimum of 3 hours before moving to tissue processor where first 2 solutions are a total of 3 hours in formalin. Although most bone marrow biopsies are received by 2:00 - 3:00pm; we've had some received as late as 4:00pm with good results. Our clinicians place the bone marrow core in pre-filled formalin at the point of collection, also. The Cal Rite is a combination of formaldehyde, formic acid and methanol. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry Sent: Friday, January 14, 2011 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcifying bone marrows after processing I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 From alisha <@t> ka-recruiting.com Mon Jan 17 14:00:59 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Mon Jan 17 13:59:05 2011 Subject: [Histonet] Great Histo Jobs Message-ID: <180912163.1295294459513.JavaMail.cfservice@sl4app3> Hi Histonet Members, I am a healthcare recruiter, specifically focused on the laboratory/pathology space. I have openings all across the country and would be interested in speaking with anyone interested in searching for a new job. Below is a great new opportunity that I am working on: Histotech and Histology Supervisor Opening in Maine: I am currently working on an amazing opportunity with one of largest most sophisticated labs in the Northeast. It is located in coastal Maine. This company is looking to hire on a Histology Supervisor and a Histotech. They currently are looking for someone with a Bachelor's Degree, histology experience, HTL(ASCP) or HT(ASCP) certified, and supervisory experience for the supervisor position. The compensation package is fantastic and includes health, dental, a retirement plan, and relocation assistance, if needed. If interested in learning more details, please email me at alisha@ka-recruiting.com. If you are not interested but know of someone you could recommend for this position, please pass on their contact information to me. We have a referral bonus for anyone you refer to me that I place into a new job. I am also working on the following histology/pathology jobs: * Technical Support Specialist - Western territory (need PA or HTL license) * Technical Support Specialist - PA/NY territory (need PA or HTL license) * Technical Support Specialist - Southeastern territory (need PA or HTL license) * MI - Dermpath Histology Manager * OK - Histology Supervisor * NV - Histotechnologist 3rd shift * NY - Long Island - Dermpath Lab Manager * NH - Histology Supervisor * Eastern OH - Histology Supervisor * Maine - Histotechnologist * Maine - Histology Supervisor * FL - Histotechnologist * NY City - Histology Supervisor with IHC experience * NY City - Histotechnologist * NY City - Grossing Tech * NV - Pathologist's Assistant If interested in any of the opportunities above, please email me. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From mhale <@t> carisls.com Mon Jan 17 14:41:42 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Jan 17 14:41:47 2011 Subject: [Histonet] Great HT Position in Ohio Message-ID: <6F33D8418806044682A391273399860F069E6C24@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamargasto.com. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From DianaRip1 <@t> aol.com Mon Jan 17 17:43:06 2011 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Mon Jan 17 17:43:20 2011 Subject: [Histonet] Job in Bay Area Message-ID: <8c485.538521c8.3a662e0a@aol.com> Histology Tech on Call at John Muir Health. Must be able to work both the Concord Campus and Walnut Creek Campus. Must be available to work between 4:00 am. and 1:30 p.m. Mostly cutting and embedded. Ability to Gross preferred. From louise.renton <@t> gmail.com Tue Jan 18 03:59:12 2011 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Jan 18 03:59:18 2011 Subject: [Histonet] cryojane slide residue Message-ID: Hello alll! I am just starting to learn about cutting cryojane sections. I was given a sample of "starfrost 4x" and 5x as part of the installation. It is my understanding that these are more"adhesive" than the ordinary "snowcoat" slides. HOWEVER, when i used these i noticed that there was a residue on the slide that stained up with eosin in a rather messy way. Is this normal? Can I get rid of it somehow? Also....I am trying to cut sections of hydroxy apatite bone substitute on the cryostat. Even with the tape transfer method the implant disintigrates - is there any way to prevent this? looking forward to some answers best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From ccpath <@t> gmail.com Tue Jan 18 08:02:06 2011 From: ccpath <@t> gmail.com (j k) Date: Tue Jan 18 08:02:10 2011 Subject: [Histonet] Ventana HPV Message-ID: Is anyone out there still using Ventana's high risk HPV probe (ISH) for gyn cytology? I would love to hear feedback on this methodology. Thanks for your time. Angel B. From alisha <@t> ka-recruiting.com Tue Jan 18 09:32:26 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Jan 18 09:32:05 2011 Subject: [Histonet] NY histotech jobs Message-ID: <214152441.1295364746668.JavaMail.cfservice@SL4APP4> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From GDawson <@t> dynacaremilwaukee.com Tue Jan 18 09:51:02 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Jan 18 09:51:06 2011 Subject: [Histonet] OCT 3/4 Message-ID: All, My docs are asking that I work up an OCT 3/4 antibody. Can anyone give me their opinion on a good one? I'm not finding a stand out in my research so I'm looking for some opinions from those in the trenches. Thanx in Advance, Glen Dawson BS, HT(ASCP), QIHC IHC Manager Milwaukee, WI From cpyse <@t> x-celllab.com Tue Jan 18 10:07:35 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Jan 18 10:07:48 2011 Subject: [Histonet] Viper & Affrim Message-ID: <001e01cbb729$d26704a0$77350de0$@com> Hello Histonetters We have recently added the BD Viper and Affrim testing to our lab. Training is underway. I would like input from the experts out there on how much time is actually spent on setting up both the Viper and Affrim testing. I know what the manufacturer says I just want the opinion of someone who is actually performing the testing. I need to consider the scheduling changes I need to make. Thanks in advance for any help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com From mhale <@t> carisls.com Tue Jan 18 10:09:54 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Jan 18 10:10:02 2011 Subject: [Histonet] OHIO HT Position Message-ID: <6F33D8418806044682A391273399860F069E735F@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamargasto.com. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From kryan <@t> nfderm.com Tue Jan 18 11:19:51 2011 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Tue Jan 18 11:19:56 2011 Subject: [Histonet] Looking for a product Message-ID: Hi Everyone, I am looking for a product call "mini fan pads". They are a pad that will neutralize formalin. I can't remember who sells them. Any help would be appreciated. Last time I saw them they came like a roll of paper towels. Thanks in advance, Kaye Ryan From flnails <@t> texaschildrens.org Tue Jan 18 11:28:25 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Jan 18 11:28:35 2011 Subject: [Histonet] RE: Looking for a product In-Reply-To: References: Message-ID: I think I recall that statlab or mercedes medical carried that item. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Tuesday, January 18, 2011 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for a product Hi Everyone, I am looking for a product call "mini fan pads". They are a pad that will neutralize formalin. I can't remember who sells them. Any help would be appreciated. Last time I saw them they came like a roll of paper towels. Thanks in advance, Kaye Ryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From billodonnell <@t> catholichealth.net Tue Jan 18 11:37:11 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Jan 18 11:37:24 2011 Subject: [Histonet] Looking for a product In-Reply-To: References: Message-ID: We get ours from Newcomer, but I have seen them in other catalogs - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Tuesday, January 18, 2011 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for a product Hi Everyone, I am looking for a product call "mini fan pads". They are a pad that will neutralize formalin. I can't remember who sells them. Any help would be appreciated. Last time I saw them they came like a roll of paper towels. Thanks in advance, Kaye Ryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Tue Jan 18 12:19:00 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Tue Jan 18 12:19:16 2011 Subject: [Histonet] RE: OCT 3/4 In-Reply-To: References: Message-ID: Have used Santa Cruz' mouse Oct 3/4 cat. sc-5279 on Formalin fixed paraffin embedded human tissue. 1:25 with labeled polymer from Dako. Dana Settembre -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, January 18, 2011 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OCT 3/4 All, My docs are asking that I work up an OCT 3/4 antibody. Can anyone give me their opinion on a good one? I'm not finding a stand out in my research so I'm looking for some opinions from those in the trenches. Thanx in Advance, Glen Dawson BS, HT(ASCP), QIHC IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kryan <@t> nfderm.com Tue Jan 18 12:45:37 2011 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Tue Jan 18 12:45:41 2011 Subject: [Histonet] Thanks so much! Message-ID: Thanks to everyone for their help with the mini fan pads!! Kaye From DSiena <@t> statlab.com Tue Jan 18 13:09:28 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Tue Jan 18 13:09:39 2011 Subject: [Histonet] RE: Looking for a product In-Reply-To: References: Message-ID: Hi Kaye, StatLab Medical Products sells the mini-fad pads and our customer service number is 800-442-3573. Thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010? x229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Tuesday, January 18, 2011 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for a product Hi Everyone, I am looking for a product call "mini fan pads". They are a pad that will neutralize formalin. I can't remember who sells them. Any help would be appreciated. Last time I saw them they came like a roll of paper towels. Thanks in advance, Kaye Ryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From casperhempel <@t> gmail.com Tue Jan 18 14:59:40 2011 From: casperhempel <@t> gmail.com (Casper Hempel) Date: Tue Jan 18 14:59:44 2011 Subject: [Histonet] Microwave oven Message-ID: Dear Histonetters We are about to purchase a new microwave oven in our lab for HIER of FFPE tissue. Do you have any recommendations? I'm only aware of EMS that sells an oven with temperature control. Any suggestions are welcome Cheers Casper From sgoebel <@t> mirnarx.com Wed Jan 19 08:35:05 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Jan 19 08:35:09 2011 Subject: [Histonet] Service Message-ID: Hello all, I am in Austin Texas and would like to know who people use to service their equipment. I have used Biomed before and wasn't really impressed. Are there any other companies? The only thing I would really ever need service on would be the microtome (brand new Thermo 325). I'm just trying to see whether a maintenance contract would be more cost effective to pay yearly than to get someone out if something goes wrong with the tome. Since it's just a plain jane rotary microtome, usually things don't really ever go wrong so I think I know the answer, but nonetheless would like repair guy/gal information. Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From wdesalvo.cac <@t> hotmail.com Wed Jan 19 09:03:39 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jan 19 09:03:44 2011 Subject: [Histonet] Service In-Reply-To: References: Message-ID: Being from a large large with multiple instrument types and multiple units of many instruments, I find it is worth asking the manufacturer to train your on-site BioMed personnel. Repairing and maintaining Microtomes can be successfully accomplished by properly trained individuals, manufacturer, contract or on-site. You can also seek an independent contractor, but be careful when choosing. Make sure small companies do not have to large territory or are sub-contracted to the major manufacturers as this can cause friction and create turn around time issues to get your instrument fixed. Independent contractors do a great job, just make sure you are getting the service you need. When considering having your BioMed trained, a key factor in this process is to have your BioMed department receive the same training the manufacturer provides to their own and contract service personnel. Depending on your relationship with the manufacturer, BioMed training can be negotiated and there is a wide range for the cost. The key point is you develop a different relationship with the manufacturer that is more of a partnership. We have been successful with BioMed being trained for microtomes, microscopes, conventional tissues processors and H&E stainer/coverslip instruments. I do not suggest this type of service for instruments that are more complex and use a specific reagent set to operate. Once you get into that complexity, there are to many variables to consider to maintain proper performance and I believe the manufacturer is best at this level. When utilizing on-site and properly trained BioMed, down time is greatly reduced, cost of parts are nominal and ordered as needed and your total service cost can be reduced. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 19 Jan 2011 08:35:05 -0600 > From: sgoebel@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Service > > Hello all, > > I am in Austin Texas and would like to know who people use to service > their equipment. I have used Biomed before and wasn't really impressed. > Are there any other companies? The only thing I would really ever need > service on would be the microtome (brand new Thermo 325). I'm just > trying to see whether a maintenance contract would be more cost > effective to pay yearly than to get someone out if something goes wrong > with the tome. Since it's just a plain jane rotary microtome, usually > things don't really ever go wrong so I think I know the answer, but > nonetheless would like repair guy/gal information. > > Thanks > > > > Sarah Goebel, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Wed Jan 19 09:34:25 2011 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Wed Jan 19 09:36:55 2011 Subject: [Histonet] Background problems with frozen liver IHC Message-ID: <5F338719C0D0DE44BDFDD2B83D3FF7A1CB76395B16@UOS-CL-EX7-L3.soton.ac.uk> Hi All, I do a lot of fluorescence labelling of frozen mouse tissue (mostly spleens) but recently I've been trying to do IHC staining of various mouse (and human) tissues. I'm having particular trouble with liver samples with high brown background in the hepatocytes - the vascular areas are completely clean. I've been using Vector Labs avidin/biotin blocking system and their ABC detection method along with DAB (Dako) and various biotinylated secondary antibodies. There is background from the secondary antibodies and to a lesser extent from the ABC. Any tips? Thanks Sonya From sonya.martin <@t> soton.ac.uk Wed Jan 19 09:39:59 2011 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Wed Jan 19 09:40:11 2011 Subject: [Histonet] PVP method for frozen bone sectioning Message-ID: <5F338719C0D0DE44BDFDD2B83D3FF7A1CB76395B17@UOS-CL-EX7-L3.soton.ac.uk> Hi all, After searching the internet I found a method for preparing mouse bones for frozen sectioning. I've been trying it out but have been having problems with the PVP coming out of solution (7.5% PVP-40T, 10% EDTA Na2 in 0.1M Tris.HCl pH6.95). I heated it to ~50-60oC to get it to dissolve but when its left in the fridge a ppt develops and the mouse femur that I have decalcifying in it is covered in large white blobs (however the decalcification does seem to be working ok). Does anyone have any experience of doing frozen bone sections this way? Any advice greatly appreciated. Thanks Sonya From sgoebel <@t> mirnarx.com Wed Jan 19 09:52:40 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Jan 19 09:52:43 2011 Subject: [Histonet] Caspase Message-ID: Look...I am a plethora of questions today!! Has anyone ever used the antibody Caspase? I hear lymph node is a good positive control, but where have people purchased the antibody, and at what dilution for FFPE tissue? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From jshelley <@t> sanfordburnham.org Wed Jan 19 10:25:31 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Wed Jan 19 10:25:37 2011 Subject: [Histonet] RE: Caspase In-Reply-To: References: Message-ID: Hi Sarah, I have used Cell Signaling antibody for Cleaved Caspase-3 (Asp175) Antibody #9661 is that what you are looking to do. I used it at a 1:100 dilution for 1 hr no heat with High pH. John S. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, January 19, 2011 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Caspase Look...I am a plethora of questions today!! Has anyone ever used the antibody Caspase? I hear lymph node is a good positive control, but where have people purchased the antibody, and at what dilution for FFPE tissue? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Wed Jan 19 10:28:36 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Jan 19 10:28:40 2011 Subject: [Histonet] RE: Caspase In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC0CFA768323@CMHEXCC01MBX.childrensmemorial.org> References: <7111DB39D045004C9CF29E79C71B28BC0CFA768323@CMHEXCC01MBX.childrensmemorial.org> Message-ID: I cc'd (is that a word?) the histonet for you. If you ever want to pose a question to everyone email: histonet@lists.utsouthwestern.edu. PPE: Of course everyone has different levels of PPE to be worn, but I think the standard rule is... Gloves Lab Coat Mask (especially if cutting frozen lung tissue, it could have TB) Eye Wear You have to remember you need all of this because once the tissue hits room temperature it is basically fresh unfixed tissue again. I have cut frozen sections with no mask before and it was all ok, but I always wore one with lung frozens. Good Luck, hope this helped Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: Setlak, Lisa [mailto:LSetlak@childrensmemorial.org] Sent: Wednesday, January 19, 2011 10:08 AM To: Sarah Goebel Subject: RE: Caspase Hi Sarah, I don't have an answer to your question but was wondering if you could post one for me- I've tried multiple times and it doesn't seem to go through. Would you be able to tell me how to pose a question to the whole group or could you possibly post one "What type of PPE is normally worn when performing frozen sections"? Thanks in advance for your help. Lisa Van Valkenberg Histology Mgr. Children's Memorial Hospital Chg,. IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, January 19, 2011 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Caspase Look...I am a plethora of questions today!! Has anyone ever used the antibody Caspase? I hear lymph node is a good positive control, but where have people purchased the antibody, and at what dilution for FFPE tissue? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Wed Jan 19 11:59:12 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Jan 19 11:59:17 2011 Subject: [Histonet] H&E Stain Message-ID: <1872B4A455B7974391609AD8034C79FC026DFD3A@LBEXCH01.hchd.local> Hello to all in histoland and Happy New Year. We are having issues with our H&E stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From mpence <@t> grhs.net Wed Jan 19 12:09:51 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jan 19 12:09:55 2011 Subject: [Histonet] H&E Stain In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD3A@LBEXCH01.hchd.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B09@is-e2k3.grhs.net> More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Stain Hello to all in histoland and Happy New Year. We are having issues with our H&E stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Jan 19 12:34:35 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jan 19 12:35:24 2011 Subject: [Histonet] H&E Stain In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974B09@is-e2k3.grhs.net> Message-ID: We have been having the same problem recently. We have tried extending the washes after the hematoxylin, agitation and adding an additional wash. Nothing has helped. It is not every slide as you say, but random ones. We are using Gill 3 and eosin from Stat Lab. We have changed the stainer and processor a number of times since this began. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mike Pence Sent: Wednesday, January 19, 2011 1:10 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stain More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Stain Hello to all in histoland and Happy New Year. We are having issues with our H&E stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From cmconway <@t> usgs.gov Wed Jan 19 14:15:56 2011 From: cmconway <@t> usgs.gov (Carla M Conway) Date: Wed Jan 19 14:15:59 2011 Subject: [Histonet] alternative to Bouin's in Schiff-methylene blue nucleic acid Message-ID: Hello, A colleague is trying to determine the nucleic acid type of a rickettsial phage. Acridine orange with RNAse/DNAse treatment was not successful. They want to try a Schiff-methylene blue technique which requires hydrolyzing in Bouin's fluid (1 h at 60 C). Could anyone suggest another protocol which does not use picric acid (or suggest an alternative to Bouin's )? Also, any advice for nucleic acid differential staining would be appreciated. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street. Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov From alisha <@t> ka-recruiting.com Wed Jan 19 15:08:35 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Wed Jan 19 15:08:25 2011 Subject: [Histonet] Histology Job Opportunities NY Message-ID: <1482470501.1295471315465.JavaMail.cfservice@SL4APP4> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From brian <@t> prometheushealthcare.com Wed Jan 19 15:27:33 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Wed Jan 19 15:27:45 2011 Subject: [Histonet] Prometheus Healthcare Lab Openings in New York Message-ID: <00cb01cbb81f$b2f5a0d0$18e0e270$@com> I hope you are having a great day. My name is Brian Feldman and I am with Prometheus Healthcare. We are a nationwide executive search firm that specializes in the healthcare industry. We are committed to connecting dedicated healthcare professionals with top medical organizations nationwide. I wanted to reach out to you in regards to a few new positions that I am currently working on. I have listed some of our hottest lab positions below: 1) Hospital in NYC - Histotech night shift 12-8am 10k sign on bonus Also have available an Evening Grossing Tech hours are from 4pm-12am 2) Private lab in Suffern, NY IHC Specialist (am), Lead Histotech (8-4 T-S), Histotechs for am T-S & 3-11 M-f Extremely competitive pay 3) Expanding Reference lab in Westchester County Histotech II Histology 3pm - 11:30pm T-S Histotech III IHC 12am-8:30am,M-F Histotech II IHC 7am-3:30pm, T-S Histotech II IHC 8am-4:30pm, M_F Cyto Prep Tech Cytology 10pm-6:30am, M-F 4) Hospital in New Rochelle, NY Medical Technologist Supervisor for evening shift Covering areas :Hematology, chemistry, blood bank Shift is 4:30pm to 12:30am 5) Reference lab in Teterboro, NJ Cytogenetic Technologist to work second shift (4PM start time). Must have FISH experience. 6) Full time Pathologist Assistant Hospital in Bay Shore, NY Histology tech background for a Supervisory role salary range based on experience Mornings.. 7:00am start 7) Histology tech Hospital in Bay Shore, NY Day shift, 7a-3p OR 8a-4p If you might know anyone who would be interested in any of the positions listed above I would greatly appreciate it. Happy New Year! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian @prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From smees001 <@t> umn.edu Wed Jan 19 15:32:18 2011 From: smees001 <@t> umn.edu (Branden Smeester) Date: Wed Jan 19 15:32:22 2011 Subject: [Histonet] Autofluorescence using Mouse Tibia Tissue Message-ID: Hello Histonetters, Currently, I am having trouble achieving good immunohistochemistry results using frozen mouse tibia sections. I use the protocol as follows: 4% PFA or 10% NBF 12-24 hrs cryoprotect in 20% sucrose until tissue sinks freeze tissue in block molds using OCT compound and isopentane/dry ice slice on cryostat (anywhere from 5-100um depending on microscopy work being completed) IHC: block (2-10% normal serum in TPBS) primary overnight PBS washes secondary PBS washes fluorescent mounting media I am using Cy3 labeled antibodies and not achieving that good staining I would like. Does anybody know a protocol or some special tricks I can use to avoid the autofluorescence? I can't seem to have any luck since slicing mouse bone tissue. I know many people have problems, but many publications have achieved good results. Thanks, Branden From jkiernan <@t> uwo.ca Wed Jan 19 15:53:19 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Jan 19 15:53:23 2011 Subject: [Histonet] alternative to Bouin's in Schiff-methylene blue nucleic acid In-Reply-To: References: Message-ID: The hot Bouin will be working as a fixative for the bacteria in addition to causing partial hydrolysis of DNA and (probably) complete hydrolysis and dissolution of RNA. Bouin is the traditional fixative for making nuclear material (nucleoids) of bacteria visible. See Robinow C & Kellenberger E 1994 "The bacterial nucleoid revisited" Microbiological Reviews 58(2):211-232. John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = ----- Original Message ----- From: Carla M Conway Date: Wednesday, January 19, 2011 15:17 Subject: [Histonet] alternative to Bouin's in Schiff-methylene blue nucleic acid To: histonet@lists.utsouthwestern.edu > Hello, > > A colleague is trying to determine the nucleic acid type of a > rickettsial > phage. Acridine orange with RNAse/DNAse treatment was not > successful. They > want to try a Schiff-methylene blue technique which requires > hydrolyzing > in Bouin's fluid (1 h at 60 C). Could anyone suggest another > protocol > which does not use picric acid (or suggest an alternative to > Bouin's )? > > Also, any advice for nucleic acid differential staining would be > appreciated. > > Thanks very much, > > > > Carla Conway > Histology Technician > Western Fisheries Research Center, USGS > 6505 N.E. 65th Street. > Seattle, WA 98115-5016 USA > Phone: 206-526-6282 ext. 242 > Fax: 206-526-6654 > E-mail: cmconway@usgs.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Jan 19 15:58:57 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Jan 19 15:59:02 2011 Subject: [Histonet] H&E Stain Message-ID: Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = ----- Original Message ----- From: "Scott, Allison D" Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] H&E Stain To: histonet@lists.utsouthwestern.edu > Hello to all in histoland and Happy New Year. We are > having issues with > our H&E stain. The nuclei are staining very blue to purple > and the > mucin is staining blue to purple-blue. It is difficult to > see the > nuclear detail. The mucin is obscuring things. We > have not changed our > process for staining or processing. The funny thing is > that it is only > in the Biopsy cases, and it is every few slides. The > surgical cases > are all right. We checked the alcohol and xylene for > water, and there > is not any. My tech changed out the stain and we are > staining a new > batch of slides. If anyone has any idea what is wrong, any > help would > be greatly appreciated. I have gone over our processes and > nothing has > changed. The reagents are the same, the staining times are > the same, > and the processing times are the same. We are using the > Shandon Gemini > stainer and VIP processor. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately > notify the > sender by return e-mail and delete this e-mail and any > attachments from > your computer system. > > To the extent the information in this e-mail and any attachments > contain > protected health information as defined by the Health Insurance > Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or > privileged. This e-mail may also be confidential and/or > privileged under > Texas law. The e-mail is for the use of only the > individual or entity named > above. If you are not the intended recipient, or any > authorized > representative of the intended recipient, you are hereby > notified that any > review, dissemination or copying of this e-mail and its > attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wgray19 <@t> sc.rr.com Wed Jan 19 16:43:02 2011 From: wgray19 <@t> sc.rr.com (Jeff and Wanda Gray) Date: Wed Jan 19 16:43:10 2011 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <002801cbb82a$3b093ef0$b11bbcd0$@rr.com> I would strongly suggest that you include cut resistant gloves as well. These are usually plastic polymer of one sort or another. At my institution we put on a pair of nitrile gloves, then the cut resistant ones, then another pair of nitrile on size larger than we usually wear. Yes, it's a lot to have on your, but yes, the extra safety is worth it. Many vendors sell these, they are not hard to find at all. Thanks, Wanda S. Gray ------------------------------ Message: 11 Date: Wed, 19 Jan 2011 10:28:36 -0600 From: Subject: [Histonet] RE: Caspase To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" I cc'd (is that a word?) the histonet for you. If you ever want to pose a question to everyone email: histonet@lists.utsouthwestern.edu. PPE: Of course everyone has different levels of PPE to be worn, but I think the standard rule is... Gloves Lab Coat Mask (especially if cutting frozen lung tissue, it could have TB) Eye Wear You have to remember you need all of this because once the tissue hits room temperature it is basically fresh unfixed tissue again. I have cut frozen sections with no mask before and it was all ok, but I always wore one with lung frozens. Good Luck, hope this helped Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: Setlak, Lisa [mailto:LSetlak@childrensmemorial.org] Sent: Wednesday, January 19, 2011 10:08 AM To: Sarah Goebel Subject: RE: Caspase Hi Sarah, I don't have an answer to your question but was wondering if you could post one for me- I've tried multiple times and it doesn't seem to go through. Would you be able to tell me how to pose a question to the whole group or could you possibly post one "What type of PPE is normally worn when performing frozen sections"? Thanks in advance for your help. Lisa Van Valkenberg Histology Mgr. Children's Memorial Hospital Chg,. IL From jnocito <@t> satx.rr.com Wed Jan 19 16:58:33 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 19 16:59:02 2011 Subject: [Histonet] Consult Fees for IHC Message-ID: Greetings histo land, Hope everyone is having a great New Year. I have been asked to consult on IHC to a local firm. They asked me what my fees are. I told them we can talk about that later. I have no idea what to charge these people. I want to be fair, but I don't want to give this knowledge away (an y'all thought that this was just a petty face). Any ideas for the Texas area. This would be telephone and on site consulting for a research firm. Thanks in advance. Joe From liz <@t> premierlab.com Wed Jan 19 17:05:02 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 19 17:05:06 2011 Subject: [Histonet] Consult Fees for IHC In-Reply-To: Message-ID: Joe Keep in mind that that what you charge you need to take into consideration the taxes you will be responsible for paying. Most consultants are paid a simple fee and the company paying you will not take out any taxes. So you need to account for that. I have known quite a few individuals who got themselves in a bind when it came to tax time. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, January 19, 2011 3:59 PM To: Histonet Subject: [Histonet] Consult Fees for IHC Greetings histo land, Hope everyone is having a great New Year. I have been asked to consult on IHC to a local firm. They asked me what my fees are. I told them we can talk about that later. I have no idea what to charge these people. I want to be fair, but I don't want to give this knowledge away (an y'all thought that this was just a petty face). Any ideas for the Texas area. This would be telephone and on site consulting for a research firm. Thanks in advance. Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Jan 19 17:33:38 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jan 19 17:33:40 2011 Subject: [Histonet] Microwave oven Message-ID: Hi, Please don't! Microwaves are horrible for HIER. For retrieval purposes, you need to get the buffer temperature up to a point and keep it there for a length of time without the solution boiling over and leaving you with unhappy dry sections. Microwaves heat unevenly really fast then boil over. The solution to this is to nuke it then cool it then nuke it again repeating until you figure it is done. Lab grade microwaves do the same thing under a bit tighter control. This results in a wave of temperature fluctuation which is anything but standardizable (if that's a word). You would be much better off getting a vegetable steamer on the cheap side, or a standard laboratory waterbath on the expensive side. These both can allow a direct monitoring of temperature throughout the retrieval process. Pressure cookers are viable options as they don't allow the buffers to boil over. Biocare Medical has a decent one as well as temperature strips that allow you to know if the temperature got to a certain point and didn't exceed another. Honestly with all CAP's nonsensical prattle about standardisation in labs I can't understand how they allow these monstrosities in modern medical care. Message: 4 Date: Tue, 18 Jan 2011 21:59:40 +0100 From: Casper Hempel Subject: [Histonet] Microwave oven To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear Histonetters We are about to purchase a new microwave oven in our lab for HIER of FFPE tissue. Do you have any recommendations? I'm only aware of EMS that sells an oven with temperature control. Any suggestions are welcome Cheers Casper From DSiena <@t> statlab.com Wed Jan 19 18:13:58 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed Jan 19 18:14:02 2011 Subject: [Histonet] H&E Stain In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C03974B09@is-e2k3.grhs.net> Message-ID: Hi Toni, I would love to speak with you about the issues that you are having, could you give me a call? I am traveling tomorrow but will be back in the office on Friday. thanks Debbie Siena Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 800-442-3573 ext 229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, January 19, 2011 12:35 PM To: Mike Pence; Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stain We have been having the same problem recently. We have tried extending the washes after the hematoxylin, agitation and adding an additional wash. Nothing has helped. It is not every slide as you say, but random ones. We are using Gill 3 and eosin from Stat Lab. We have changed the stainer and processor a number of times since this began. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mike Pence Sent: Wednesday, January 19, 2011 1:10 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stain More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Stain Hello to all in histoland and Happy New Year. We are having issues with our H&E stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From gloria.cole <@t> usa.net Wed Jan 19 19:35:36 2011 From: gloria.cole <@t> usa.net (Gloria Cole) Date: Wed Jan 19 19:35:43 2011 Subject: [Histonet] Histology position, Irving, TX Message-ID: <600BCAD3-1D8F-40F8-B941-4A8B3DD3AAC2@usa.net> Hello everyone, I will have a full time histology position available within a couple of weeks and was looking for a certified technician with grossing experience. This is a day time position and may be starting at 8 am or 9am. If anyone is interested or know someone who is interested, please contact me. Thank you very much, Gloria Cole Histology Supervisor NuePath Laboratory gcole@nueterrapathology.com gloria.cole@usa.net From traczyk7 <@t> aol.com Wed Jan 19 23:10:03 2011 From: traczyk7 <@t> aol.com (traczyk7@aol.com) Date: Wed Jan 19 23:10:08 2011 Subject: [Histonet] Microwave oven In-Reply-To: References: Message-ID: <8CD86776E224722-1854-62CD@webmail-d003.sysops.aol.com> Amos, I think you have a few misconceptions about microwave technology. Lumping laboratory grade units in with household appliances is (in my opinion) unfair. Temperature control is essential and there are microwave processors available that do it quite well. Also, how in the world can all of the buffer solution "boil out" of a close container? Have you looked at the modules provided by Milestone or Hacker? They all have lids! Check out the mw credentials of a company in North Carolina called CEM. Their research and manufacturing in the field of microwave technology is some of the best in the world. There are always pros & cons to be considered when making equipment purchases but I believe the large net you cast damning microwave technology is short sighted. Anyone else agree? Dorothy Dorothy Traczyk MTA Histology LLC Point Pleasant, NJ 08742 -----Original Message----- From: Amos Brooks To: casperhempel ; histonet Sent: Wed, Jan 19, 2011 6:34 pm Subject: [Histonet] Microwave oven Hi, Please don't! Microwaves are horrible for HIER. For retrieval purposes, ou need to get the buffer temperature up to a point and keep it there for a ength of time without the solution boiling over and leaving you with nhappy dry sections. Microwaves heat unevenly really fast then boil over. he solution to this is to nuke it then cool it then nuke it again repeating ntil you figure it is done. Lab grade microwaves do the same thing under a it tighter control. This results in a wave of temperature fluctuation which s anything but standardizable (if that's a word). You would be much better off getting a vegetable steamer on the cheap ide, or a standard laboratory waterbath on the expensive side. These both an allow a direct monitoring of temperature throughout the retrieval rocess. Pressure cookers are viable options as they don't allow the buffers o boil over. Biocare Medical has a decent one as well as temperature strips hat allow you to know if the temperature got to a certain point and didn't xceed another. Honestly with all CAP's nonsensical prattle about tandardisation in labs I can't understand how they allow these onstrosities in modern medical care. Message: 4 ate: Tue, 18 Jan 2011 21:59:40 +0100 rom: Casper Hempel ubject: [Histonet] Microwave oven o: histonet@lists.utsouthwestern.edu essage-ID: ontent-Type: text/plain; charset=ISO-8859-1 Dear Histonetters e are about to purchase a new microwave oven in our lab for HIER of FFPE issue. Do you have any recommendations? I'm only aware of EMS that sells an ven with temperature control. Any suggestions are welcome heers asper ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Wed Jan 19 23:25:40 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jan 19 23:25:43 2011 Subject: [Histonet] H&E Stain In-Reply-To: References: Message-ID: I agree that the pH might be high, but I also suggest you check your water rinse on the stainer. If you are using "tap" water, there can be a significant fluctuation in the quality of the water and the amount of additives and impurities present at any one time can also contribute to the mucin not being rinsed away and staining. If you are using tap water, changing to distilled or dionized water might help to improve the consistency of stain results. Good luck William DeSalvo, B.S., HTL(ASCP) > From: jkiernan@uwo.ca > To: Allison_Scott@hchd.tmc.edu > Date: Wed, 19 Jan 2011 16:58:57 -0500 > Subject: Re: [Histonet] H&E Stain > CC: histonet@lists.utsouthwestern.edu > > Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. > > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = > ----- Original Message ----- > From: "Scott, Allison D" > Date: Wednesday, January 19, 2011 13:01 > Subject: [Histonet] H&E Stain > To: histonet@lists.utsouthwestern.edu > > > Hello to all in histoland and Happy New Year. We are > > having issues with > > our H&E stain. The nuclei are staining very blue to purple > > and the > > mucin is staining blue to purple-blue. It is difficult to > > see the > > nuclear detail. The mucin is obscuring things. We > > have not changed our > > process for staining or processing. The funny thing is > > that it is only > > in the Biopsy cases, and it is every few slides. The > > surgical cases > > are all right. We checked the alcohol and xylene for > > water, and there > > is not any. My tech changed out the stain and we are > > staining a new > > batch of slides. If anyone has any idea what is wrong, any > > help would > > be greatly appreciated. I have gone over our processes and > > nothing has > > changed. The reagents are the same, the staining times are > > the same, > > and the processing times are the same. We are using the > > Shandon Gemini > > stainer and VIP processor. > > > > Allison Scott HT(ASCP) > > Histology Supervisor > > LBJ Hospital > > Houston, Texas > > CONFIDENTIALITY NOTICE: > > If you have received this e-mail in error, please immediately > > notify the > > sender by return e-mail and delete this e-mail and any > > attachments from > > your computer system. > > > > To the extent the information in this e-mail and any attachments > > contain > > protected health information as defined by the Health Insurance > > Portability > > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > > Parts 160 and > > 164; or Chapter 181, Texas Health and Safety Code, it is > > confidential and/or > > privileged. This e-mail may also be confidential and/or > > privileged under > > Texas law. The e-mail is for the use of only the > > individual or entity named > > above. If you are not the intended recipient, or any > > authorized > > representative of the intended recipient, you are hereby > > notified that any > > review, dissemination or copying of this e-mail and its > > attachments is > > strictly prohibited. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Thu Jan 20 08:17:46 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Jan 20 08:18:39 2011 Subject: [Histonet] H&E Stain In-Reply-To: Message-ID: Our tap water consistently reads 6.0, and has for years. We did try turning off the tap when this first began, and manually rinsing with distilled water, but saw no difference. I will try adding the HCl today with a few test slides. Will let you know how this works out. Thanks for the suggestions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of WILLIAM DESALVO Sent: Thursday, January 20, 2011 12:26 AM To: jkiernan@uwo.ca; allison_scott@hchd.tmc.edu Cc: histonet Subject: RE: [Histonet] H&E Stain I agree that the pH might be high, but I also suggest you check your water rinse on the stainer. If you are using "tap" water, there can be a significant fluctuation in the quality of the water and the amount of additives and impurities present at any one time can also contribute to the mucin not being rinsed away and staining. If you are using tap water, changing to distilled or dionized water might help to improve the consistency of stain results. Good luck William DeSalvo, B.S., HTL(ASCP) > From: jkiernan@uwo.ca > To: Allison_Scott@hchd.tmc.edu > Date: Wed, 19 Jan 2011 16:58:57 -0500 > Subject: Re: [Histonet] H&E Stain > CC: histonet@lists.utsouthwestern.edu > > Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. > > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = > ----- Original Message ----- > From: "Scott, Allison D" > Date: Wednesday, January 19, 2011 13:01 > Subject: [Histonet] H&E Stain > To: histonet@lists.utsouthwestern.edu > > > Hello to all in histoland and Happy New Year. We are > > having issues with > > our H&E stain. The nuclei are staining very blue to purple > > and the > > mucin is staining blue to purple-blue. It is difficult to > > see the > > nuclear detail. The mucin is obscuring things. We > > have not changed our > > process for staining or processing. The funny thing is > > that it is only > > in the Biopsy cases, and it is every few slides. The > > surgical cases > > are all right. We checked the alcohol and xylene for > > water, and there > > is not any. My tech changed out the stain and we are > > staining a new > > batch of slides. If anyone has any idea what is wrong, any > > help would > > be greatly appreciated. I have gone over our processes and > > nothing has > > changed. The reagents are the same, the staining times are > > the same, > > and the processing times are the same. We are using the > > Shandon Gemini > > stainer and VIP processor. > > > > Allison Scott HT(ASCP) > > Histology Supervisor > > LBJ Hospital > > Houston, Texas > > CONFIDENTIALITY NOTICE: > > If you have received this e-mail in error, please immediately > > notify the > > sender by return e-mail and delete this e-mail and any > > attachments from > > your computer system. > > > > To the extent the information in this e-mail and any attachments > > contain > > protected health information as defined by the Health Insurance > > Portability > > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > > Parts 160 and > > 164; or Chapter 181, Texas Health and Safety Code, it is > > confidential and/or > > privileged. This e-mail may also be confidential and/or > > privileged under > > Texas law. The e-mail is for the use of only the > > individual or entity named > > above. If you are not the intended recipient, or any > > authorized > > representative of the intended recipient, you are hereby > > notified that any > > review, dissemination or copying of this e-mail and its > > attachments is > > strictly prohibited. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From akemiat3377 <@t> yahoo.com Thu Jan 20 08:38:23 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Jan 20 08:38:31 2011 Subject: [Histonet] Re: Thanks- CLARIFICATION: CAP #ANP.21382 Reagent Expiration Date. In-Reply-To: <355155.66640.qm@web113817.mail.gq1.yahoo.com> References: <355155.66640.qm@web113817.mail.gq1.yahoo.com> Message-ID: <65F32923-86DE-4A32-A00D-10C625120104@yahoo.com> Thank you to all the wonderful people who responded to my inquiry, It was great to see what the various protocols are in place. I will share this information, and will implement a more formalized and concise policy. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Jan 12, 2011, at 6:21 PM, Akemi Allison wrote: > Thanks Laurie, and Histoland, > > My client does what Laurie suggests. Perhaps, I need to clarify: > Please read > information below: This is regarding RAW chemicals such as: Iodine > crystals, > Sodium Thiosulfate, Mercuric Chloride, and Copper, etc, and dry Dye > Stains, that > do not have expiration dates. I don't have a written policy for > evaluation of > these chemicals and dyes that do not have an expiration date. How > do I know > they can use these chemicals and dyes to make up the final end > solutions. > > My client currently visualy inspects these chemicals and dyes to > see if they > appear to be OK, they put another year to review "visually". How > can an > untrained, non-chemist, judge by visual inspecition if the chemical > is OK??? > > I need a written policy to adhere to CAP guidelines. > Akemi Allison BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > > > > > ________________________________ > From: Laurie Colbert > To: Akemi Allison > Sent: Wed, January 12, 2011 3:17:20 PM > Subject: RE: [Histonet] CAP #ANP.21382 Reagent Expiration Date. > > Hi Akemi, > > Our procedure for evaluating stains/reagents is to run control > tissue. If the > control works, the stain/reagent is good. And that is done much > more often than > annually - it is done every time the stain is performed. > > Laurie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Akemi Allison > Sent: Wednesday, January 12, 2011 2:07 PM > To: histonet > Subject: [Histonet] CAP #ANP.21382 Reagent Expiration Date. > > Hi Everyone in Histoland! > Happy Hump day! I have 2 questions to ask you. The CAP "#ANP. > 21382 Reagent > Expiration Date", Evidence of compliance requires a written policy for > evaluating reagents lacking manufacturer's expiration date. > > I worked in the Biotech arena for several years in R&D and > manufacturing. We > had procedures which were in compliance to GLP and manufacturing > standards. > These policies were much more rigid than for AP departments, who > adher to CAP > requirements. > > > Would any of you kind people like to share a copy of your written > policy with > me. I would be forever grateful! > > Second question: How do you address powder dyes, and chemicals in > crystalin and > > powder form, which are extremely old and do not have an expiration > date? What > is your criteria for passing of failing these chemicals and dyes? > Do you > visually inspect these chemicals and dyes on an annual basis, and > if they look > fine, give it another year for a visual check with next years date, > or do you > send them out to a company for analysis to see if they past required > specifications??? That would be pretty costly! > > If anyone has a written procedure for this I would love to see it too! > > Thank you in advance for your assistance. > Akemi > Akemi Allison BS, HT(ASCP)HTL > > E-Mail: akemiat3377@yahoo.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 20 09:40:15 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 20 09:40:21 2011 Subject: [Histonet] Consult Fees for IHC In-Reply-To: Message-ID: <42740.27938.qm@web65703.mail.ac4.yahoo.com> Joe: There is sort of a "standard" payment that almost all universities?use of $3000/5 days week. This represents about $75/hour and at that rate is how most consultants work. IF they ask you for your SS number then they will issue you (at the end of the year) the correspondent IRS Form. I have never had any problems with that, you will just add that income to your 1040 Form and at the end your tax deduction will be very small. You do not have to give away your knowledge, just make sure they get their moneys worth! Ren? J. --- On Wed, 1/19/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] Consult Fees for IHC To: "Histonet" Date: Wednesday, January 19, 2011, 5:58 PM Greetings histo land, Hope everyone is having a great New Year. I have been asked to consult on IHC to a local firm. They asked me what my fees are. I told them we can talk about that later. I have no idea what to charge these people. I want to be fair, but I don't want to give this knowledge away (an y'all thought that this was just a petty face). Any ideas for the Texas area. This would be telephone and on site consulting for a research firm. Thanks in advance. Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 20 09:48:10 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 20 09:48:13 2011 Subject: [Histonet] Microwave oven In-Reply-To: <8CD86776E224722-1854-62CD@webmail-d003.sysops.aol.com> Message-ID: <951054.32348.qm@web65703.mail.ac4.yahoo.com> Dorothy: Regardless of the fact that I agree with you, never ask for the "support" others may provide to your statements. Give your honest opinion?and do not mind?what others may think about it! Ren? J. --- On Thu, 1/20/11, traczyk7@aol.com wrote: From: traczyk7@aol.com Subject: Re: [Histonet] Microwave oven To: amosbrooks@gmail.com, histonet@lists.utsouthwestern.edu Date: Thursday, January 20, 2011, 12:10 AM Amos, I think you have a few misconceptions about microwave technology.? Lumping laboratory grade units in with household appliances is (in my opinion) unfair.? Temperature control is essential and there are microwave processors available that do it quite well.? Also, how in the world can all of the buffer solution "boil out" of a close container?? Have you looked at the modules provided by Milestone or Hacker?? They all have lids! Check out the mw credentials of a company in North Carolina called CEM.? Their research and manufacturing in the field of microwave technology is some of the best in the world. There are always pros & cons to be considered when making equipment purchases but I believe the large net you cast damning microwave technology is short sighted. Anyone else agree? Dorothy Dorothy Traczyk MTA Histology LLC Point Pleasant, NJ 08742? -----Original Message----- From: Amos Brooks To: casperhempel ; histonet Sent: Wed, Jan 19, 2011 6:34 pm Subject: [Histonet] Microwave oven Hi, ? ? Please don't! Microwaves are horrible for HIER. For retrieval purposes, ou need to get the buffer temperature up to a point and keep it there for a ength of time without the solution boiling over and leaving you with nhappy dry sections. Microwaves heat unevenly really fast then boil over. he solution to this is to nuke it then cool it then nuke it again repeating ntil you figure it is done. Lab grade microwaves do the same thing under a it tighter control. This results in a wave of temperature fluctuation which s anything but standardizable (if that's a word). ? ? You would be much better off getting a vegetable steamer on the cheap ide, or a standard laboratory waterbath on the expensive side. These both an allow a direct monitoring of temperature throughout the retrieval rocess. Pressure cookers are viable options as they don't allow the buffers o boil over. Biocare Medical has a decent one as well as temperature strips hat allow you to know if the temperature got to a certain point and didn't xceed another. Honestly with all CAP's nonsensical prattle about tandardisation in labs I can't understand how they allow these onstrosities in modern medical care. Message: 4 ate: Tue, 18 Jan 2011 21:59:40 +0100 rom: Casper Hempel ubject: [Histonet] Microwave oven o: histonet@lists.utsouthwestern.edu essage-ID: ? ? ? ontent-Type: text/plain; charset=ISO-8859-1 Dear Histonetters e are about to purchase a new microwave oven in our lab for HIER of FFPE issue. Do you have any recommendations? I'm only aware of EMS that sells an ven with temperature control. Any suggestions are welcome heers asper ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Thu Jan 20 10:00:27 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Jan 20 10:00:34 2011 Subject: [Histonet] Re: H&E Stain Message-ID: Allison Scott wrote: Hello to all in histoland and Happy New Year. We are having issues with our H&E stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas When you say it is every few slides, is there variation within multiple slides of one case? If all the slides (levels) of a case look the same, I would suspect a problem with that particular case, not the processing and not the staining. Are these all GI biopsies? Or do you see this in other needle biopsies, cervical biopsies and the like? GI biopsies tend to show hazy nuclei in epithelial cells commonly. There are several explanations and mostly are attributed to inadequate fixation. It could be you a client who has changed how they collect and fix the specimens. Are they letting them dry on a gauze before putting them in fixative? It could be you have something out of place on the tissue processor. It could be there is water under the tissue on the slide and it is subject to high heat (microwave or oven) and is cooking the cells prior to staining. As pointed out previously, it could be a problem with your hematoxylin pH. Good luck, and please let us know what the fix for this is when you get it figured out! Best wishes, Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From alisha <@t> ka-recruiting.com Thu Jan 20 10:13:02 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Jan 20 10:12:27 2011 Subject: [Histonet] Histology Supervisor Job Opportunity Message-ID: <19045563.1295539981905.JavaMail.cfservice@SL4APP4> Hi Histonet Members, I am currently working on a Histology Supervisor position in Ohio. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From cgill <@t> marylandgeneral.org Thu Jan 20 10:48:46 2011 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Thu Jan 20 10:48:52 2011 Subject: [Histonet] Animal tissue processing Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9F1E@MDGEN-EXCH1.marylandgeneral.org> Hi All, I work in a hospital where we process human tissue. As a favor to a friend the pathologist would like us to process animal tissue. My questions are could we process the animal tissue on the same processor with the human tissue? And Are there different processing times and reagents for animal tissue? Thanks for any help you can give........ Caula (HT) From BSullivan <@t> shorememorial.org Thu Jan 20 10:56:30 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Thu Jan 20 10:59:54 2011 Subject: [Histonet] Animal tissue processing In-Reply-To: <087A9911BBAFDE4B8151CB148586E2C23A9F1E@MDGEN-EXCH1.marylandgeneral.org> Message-ID: Caula, I have processed my fair share of animal tissue and I used the same reagents and times that I used for human tissue. With some animal organ tissue there is the possibility of drying so you need to be careful and watch out for that. You would adjust your times accordingly. I, because of circumstances, always had the animal tissue on their own run. I do not know if there are any regulations that specifically states this. Never heard of any. I'm sure our veterinary crowd can answer that best. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Gill, Caula A." To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Animal tissue processing 01/20/2011 11:48 AM Hi All, I work in a hospital where we process human tissue. As a favor to a friend the pathologist would like us to process animal tissue. My questions are could we process the animal tissue on the same processor with the human tissue? And Are there different processing times and reagents for animal tissue? Thanks for any help you can give........ Caula (HT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jeffery.Miller <@t> spectrum-health.org Thu Jan 20 11:05:45 2011 From: Jeffery.Miller <@t> spectrum-health.org (Jeffery.Miller@spectrum-health.org) Date: Thu Jan 20 11:06:41 2011 Subject: [Histonet] Myocardial Biopsies Message-ID: My hospital has recently started performing heart transplants and we are now receiving numerous myocardial biopsies to check for rejection. One of our forensic pathologists is an expert in cardiovascular pathology and has read many biopsies over the years for other hospitals. His protocol for cutting these biopsies is very labor intensive (10 slides with 8 serial sections on each slide, some stained with H&E others kept unstained and 2 for C4d immunos). I'm curious to know what other labs are doing for these biopsies in hopes of getting this protocol changes. Thanks for your help Jeff Miller Spectrum Health Grand Rapids. MI From LSetlak <@t> childrensmemorial.org Thu Jan 20 11:10:04 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Thu Jan 20 11:10:11 2011 Subject: [Histonet] RE: Myocardial Biopsies In-Reply-To: References: Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA768346@CMHEXCC01MBX.childrensmemorial.org> Hi Jeff, I work at Children's Memorial in Chicago and we do these routinely. We cut 5 slides with 3 sections per slide. Slides 1,3,5 are for H&E and slides 2,4 we stain with the HPS stain. We do C4d but it's not done automatically. Hope this helps, Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffery.Miller@spectrum-health.org Sent: Thursday, January 20, 2011 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Myocardial Biopsies My hospital has recently started performing heart transplants and we are now receiving numerous myocardial biopsies to check for rejection. One of our forensic pathologists is an expert in cardiovascular pathology and has read many biopsies over the years for other hospitals. His protocol for cutting these biopsies is very labor intensive (10 slides with 8 serial sections on each slide, some stained with H&E others kept unstained and 2 for C4d immunos). I'm curious to know what other labs are doing for these biopsies in hopes of getting this protocol changes. Thanks for your help Jeff Miller Spectrum Health Grand Rapids. MI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Jan 20 11:16:45 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jan 20 11:16:49 2011 Subject: [Histonet] RE: Myocardial Biopsies In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408161206DD@CHEXCMS10.one.ads.che.org> We've done them for years... 6 levels on 2 slides - L1-L3, L4-L6 1st level placed near label end of the slide No unstained. We do C4d when ordered. For cases with no previous biopsies - Pick up unstained slides at level 3 for: PAS, Masson Trichrome, Iron, Congo Red and Sulfonated Alcian Blue Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffery.Miller@spectrum-health.org Sent: Thursday, January 20, 2011 12:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Myocardial Biopsies My hospital has recently started performing heart transplants and we are now receiving numerous myocardial biopsies to check for rejection. One of our forensic pathologists is an expert in cardiovascular pathology and has read many biopsies over the years for other hospitals. His protocol for cutting these biopsies is very labor intensive (10 slides with 8 serial sections on each slide, some stained with H&E others kept unstained and 2 for C4d immunos). I'm curious to know what other labs are doing for these biopsies in hopes of getting this protocol changes. Thanks for your help Jeff Miller Spectrum Health Grand Rapids. MI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From TGoins <@t> mt.gov Thu Jan 20 11:17:30 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Jan 20 11:17:40 2011 Subject: [Histonet] RE: Animal tissue processing In-Reply-To: <087A9911BBAFDE4B8151CB148586E2C23A9F1E@MDGEN-EXCH1.marylandgeneral.org> References: <087A9911BBAFDE4B8151CB148586E2C23A9F1E@MDGEN-EXCH1.marylandgeneral.org> Message-ID: The NSH has an "Animal Processing Manual" available at their WEB site. Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gill, Caula A. Sent: Thursday, January 20, 2011 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal tissue processing Hi All, I work in a hospital where we process human tissue. As a favor to a friend the pathologist would like us to process animal tissue. My questions are could we process the animal tissue on the same processor with the human tissue? And Are there different processing times and reagents for animal tissue? Thanks for any help you can give........ Caula (HT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Jan 20 11:26:29 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jan 20 11:26:40 2011 Subject: [Histonet] C3d Message-ID: <4D3829F4.7400.0077.1@harthosp.org> Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Kristopher.Kalleberg <@t> unilever.com Thu Jan 20 11:29:32 2011 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Thu Jan 20 11:29:38 2011 Subject: [Histonet] flash freezing tissue Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C09E70FB3@NTRSEVS30002.s3.ms.unilever.com> Can someone recommend the best protocol for flash freezing tissue (skin). I am unsure if fixing tissue before freezing or fixing tissue post freezing is best. Also, these samples cannot be fixed with formalin. Thanks in advance. kris From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 20 11:42:00 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 20 11:42:15 2011 Subject: [Histonet] C3d In-Reply-To: <4D3829F4.7400.0077.1@harthosp.org> References: <4D3829F4.7400.0077.1@harthosp.org> Message-ID: Richard, We have used the C3d from Cell Marque for quite some time on our Bonds with very clean crisp results on all our transplant biopsies. The jury is still out on how much more information we get in addition to C4d. Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, January 20, 2011 12:26 PM To: Histonet Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From SohrabB1 <@t> ah.org Thu Jan 20 11:49:28 2011 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Thu Jan 20 11:49:40 2011 Subject: [Histonet] NEW CAP? Message-ID: <4D380528.4347.0054.1@ah.org> ANP.22760 NEW REAGENT LOT VERIFICATION New lots of antibody and detection system reagents are tested in parallel with old lots. Record of validation of new reagents/shipments I was wondering if you are using Ventana xt, How are you testing each time you use new DAB-kit ( new lot#) Thanks, Behnaz From mab70 <@t> medschl.cam.ac.uk Thu Jan 20 11:54:09 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Thu Jan 20 11:56:55 2011 Subject: [Histonet] Animal tissue processing In-Reply-To: <087A9911BBAFDE4B8151CB148586E2C23A9F1E@MDGEN-EXCH1.marylandgeneral.org> References: <087A9911BBAFDE4B8151CB148586E2C23A9F1E@MDGEN-EXCH1.marylandgeneral.org> Message-ID: Generally rodent tissues require shorter times for processing. See the "Animal Processing Manual" published by the NSH for a range of tried and tested protocols. Below I have pasted a copy of my schedule which works well for mouse tissues. As you will see I designed it in the first instance for pancreas which tends to harden on longer processes, now I use it for all my mouse tissues, with the exception of intact heads (these require much longer, i.e. 2 hours per station.) All my processes are developed using the methods in the above mentioned manual as a guide. My processor is a "dunk and dip" type of machine, the Leica TP1020. I hope this helps. SOP 3 PROGRAMME 4: FOR MOUSE PANCREAS COSHH CBH001 STATION REAGENT DURATION VACUUM 1 Formalin/70% ethanol 30 mins Y 2 80% Ethanol 30 mins Y 3 90% Ethanol 20 mins Y 4 Absolute ethanol/IMS 20 mins Y 5 Absolute ethanol/IMS 30 mins Y 6 Absolute ethanol/IMS 30 mins Y 7 Histoclear II 20 mins Y 8 Histoclear II 30 mins Y 9 Histoclear II 30 mins Y 10 Wax 30 mins Y 11 Wax 45 mins Y 12 Wax 45 mins Y This programme can be run during the day as long as it is started early enough; if not, a delay must be set in order that the samples are not left in hot wax for extended times - refer to instrument manual. Good luck Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gill, Caula A. Sent: 20 January 2011 16:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal tissue processing Hi All, I work in a hospital where we process human tissue. As a favor to a friend the pathologist would like us to process animal tissue. My questions are could we process the animal tissue on the same processor with the human tissue? And Are there different processing times and reagents for animal tissue? Thanks for any help you can give........ Caula (HT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Thu Jan 20 11:57:33 2011 From: member <@t> linkedin.com (richard shook via LinkedIn) Date: Thu Jan 20 11:57:38 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <2140110065.21346021.1295546253999.JavaMail.app@ela4-bed37.prod> LinkedIn ------------richard shook requested to add you as a connection on LinkedIn: ------------------------------------------ Jackie, I'd like to add you to my professional network on LinkedIn. - richard Accept invitation from richard shook http://www.linkedin.com/e/yvpgd1-gj5ysfcb-e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1065146327_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPsOcPoQcjkSc359bQJclTlPinFKbPgTejsPej0Ucj8LrCBxbOYWrSlI/EML_comm_afe/ View invitation from richard shook http://www.linkedin.com/e/yvpgd1-gj5ysfcb-e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1065146327_3/3dvdP8PdzgNdjoMckALqnpPbOYWrSlI/svi/ ------------------------------------------ DID YOU KNOW you can use your LinkedIn profile as your website? Select a vanity URL and then promote this address on your business cards, email signatures, website, etc http://www.linkedin.com/e/yvpgd1-gj5ysfcb-e/ewp/inv-21/ -- (c) 2010, LinkedIn Corporation From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 20 12:03:48 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 20 12:03:53 2011 Subject: [Histonet] RE: Myocardial Biopsies In-Reply-To: References: Message-ID: 7 slides, one ribbon of 6 sections/slide: H&E x4, C4d x1, Masson Trichrome x1, unstained x1 Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffery.Miller@spectrum-health.org Sent: Thursday, January 20, 2011 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Myocardial Biopsies My hospital has recently started performing heart transplants and we are now receiving numerous myocardial biopsies to check for rejection. One of our forensic pathologists is an expert in cardiovascular pathology and has read many biopsies over the years for other hospitals. His protocol for cutting these biopsies is very labor intensive (10 slides with 8 serial sections on each slide, some stained with H&E others kept unstained and 2 for C4d immunos). I'm curious to know what other labs are doing for these biopsies in hopes of getting this protocol changes. Thanks for your help Jeff Miller Spectrum Health Grand Rapids. MI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From NSEARCY <@t> swmail.sw.org Thu Jan 20 12:15:43 2011 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Jan 20 12:15:50 2011 Subject: [Histonet] Courier "Tracking Mechanism" Message-ID: <4D38276F.5D38.00EF.0@swmail.sw.org> Am looking for a method for verification of specimens for couriers/ laboratories. Anyone use such an animal? Bill, as I recall you have in use such an animal? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 20 12:18:46 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 20 12:18:53 2011 Subject: [Histonet] Courier "Tracking Mechanism" In-Reply-To: <4D38276F.5D38.00EF.0@swmail.sw.org> References: <4D38276F.5D38.00EF.0@swmail.sw.org> Message-ID: Our lab here, and previous employ in Virginia, uses Gajema Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Thursday, January 20, 2011 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Courier "Tracking Mechanism" Am looking for a method for verification of specimens for couriers/ laboratories. Anyone use such an animal? Bill, as I recall you have in use such an animal? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mucram11 <@t> comcast.net Thu Jan 20 12:28:10 2011 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Jan 20 12:28:13 2011 Subject: [Histonet] C3d In-Reply-To: <4D3829F4.7400.0077.1@harthosp.org> Message-ID: <1777578371.865864.1295548090037.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Hi Dr Cartun, I hope anyone answering this will make it available for all of HistoNet.? I would be very interested in the use of this antibody as we are already using C4d IF for kidney here. Pamela Marcum UAMS Little Rock AR ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? ?Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT ?06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? ?Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT ?06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 20 12:33:52 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 20 12:34:02 2011 Subject: [Histonet] C3d In-Reply-To: <1777578371.865864.1295548090037.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <4D3829F4.7400.0077.1@harthosp.org> <1777578371.865864.1295548090037.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Pam, We have been performing C4d by IHC for about 3 years now and C3d for many months on all our transplant biopsies. Obviously, being able to do this on paraffin obviates the need to have additional tissue for frozens (although I do understand the need for additional IF on kidney biopsies - but we're working on that!). Currently we get both antibodies from Cell Marque and run them on our Bonds Let me know if you need any more info. Ronnie Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, January 20, 2011 1:28 PM To: Richard Cartun Cc: Histonet Subject: Re: [Histonet] C3d Hi Dr Cartun, I hope anyone answering this will make it available for all of HistoNet.? I would be very interested in the use of this antibody as we are already using C4d IF for kidney here. Pamela Marcum UAMS Little Rock AR ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? ?Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT ?06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? ?Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT ?06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mucram11 <@t> comcast.net Thu Jan 20 12:42:16 2011 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Jan 20 12:42:20 2011 Subject: [Histonet] C3d In-Reply-To: Message-ID: <261499726.866411.1295548936637.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Our patholgoists do not feel IHC gives them the discreet staining patterns they see with IF.? They have tried IHC for C4d and did not like it.? The only platform we have here is Ventana and that often slows us down on things as they may not have the antibody ready to use or a protocol for it. Pam Marcum ----- Original Message ----- From: "Ronald Houston" To: "Pamela Marcum" , "Richard Cartun" Cc: "Histonet" Sent: Thursday, January 20, 2011 12:33:52 PM Subject: RE: [Histonet] C3d Pam, We have been performing C4d by IHC for about 3 years now and C3d for many months on all our transplant biopsies. Obviously, being able to do this on paraffin obviates the need to have additional tissue for frozens (although I do understand the need for additional IF on kidney biopsies - but we're working on that!). Currently we get both antibodies from Cell Marque and run them on our Bonds Let me know if you need any more info. Ronnie Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, January 20, 2011 1:28 PM To: Richard Cartun Cc: Histonet Subject: Re: [Histonet] C3d Hi Dr Cartun, I hope anyone answering this will make it available for all of HistoNet.? I would be very interested in the use of this antibody as we are already using C4d IF for kidney here. Pamela Marcum UAMS Little Rock AR ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? ?Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT ?06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? ?Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT ?06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ----- Original Message ----- From: "Ronald Houston" To: "Pamela Marcum" , "Richard Cartun" Cc: "Histonet" Sent: Thursday, January 20, 2011 12:33:52 PM Subject: RE: [Histonet] C3d Pam, We have been performing C4d by IHC for about 3 years now and C3d for many months on all our transplant biopsies. Obviously, being able to do this on paraffin obviates the need to have additional tissue for frozens (although I do understand the need for additional IF on kidney biopsies - but we're working on that!). Currently we get both antibodies from Cell Marque and run them on our Bonds Let me know if you need any more info. Ronnie Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, January 20, 2011 1:28 PM To: Richard Cartun Cc: Histonet Subject: Re: [Histonet] C3d Hi Dr Cartun, I hope anyone answering this will make it available for all of HistoNet.? I would be very interested in the use of this antibody as we are already using C4d IF for kidney here. Pamela Marcum UAMS Little Rock AR ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? ?Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT ?06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? ?Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT ?06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Janice.Mahoney <@t> alegent.org Thu Jan 20 12:45:02 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Thu Jan 20 12:45:36 2011 Subject: [Histonet] Courier "Tracking Mechanism" In-Reply-To: References: <4D38276F.5D38.00EF.0@swmail.sw.org> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B535507C@EXCHMBC2.ad.ah.local> If you mean a tracking system, we use Ventana Vantage and love it. It is a perfect tracking system but so much more. Check it out through your Ventana rep. Jan Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Thursday, January 20, 2011 12:19 PM To: 'Nita Searcy'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Courier "Tracking Mechanism" Our lab here, and previous employ in Virginia, uses Gajema Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Thursday, January 20, 2011 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Courier "Tracking Mechanism" Am looking for a method for verification of specimens for couriers/ laboratories. Anyone use such an animal? Bill, as I recall you have in use such an animal? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From cgill <@t> marylandgeneral.org Thu Jan 20 13:01:12 2011 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Thu Jan 20 13:01:16 2011 Subject: [Histonet] Animal tissue processing In-Reply-To: References: <087A9911BBAFDE4B8151CB148586E2C23A9F1E@MDGEN-EXCH1.marylandgeneral.org> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9F1F@MDGEN-EXCH1.marylandgeneral.org> Thanks everyone the info really helps. At this time we are not sure of the exact type of tissue but I do know that it is for research. I'll check on the regulations if any about running them together. Thanks again, Those in the northeast happy snow!!!! -----Original Message----- From: BSullivan@shorememorial.org [mailto:BSullivan@shorememorial.org] Sent: Thursday, January 20, 2011 11:57 AM To: Gill, Caula A. Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Animal tissue processing Caula, I have processed my fair share of animal tissue and I used the same reagents and times that I used for human tissue. With some animal organ tissue there is the possibility of drying so you need to be careful and watch out for that. You would adjust your times accordingly. I, because of circumstances, always had the animal tissue on their own run. I do not know if there are any regulations that specifically states this. Never heard of any. I'm sure our veterinary crowd can answer that best. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Gill, Caula A." To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Animal tissue processing 01/20/2011 11:48 AM Hi All, I work in a hospital where we process human tissue. As a favor to a friend the pathologist would like us to process animal tissue. My questions are could we process the animal tissue on the same processor with the human tissue? And Are there different processing times and reagents for animal tissue? Thanks for any help you can give........ Caula (HT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Thu Jan 20 13:07:29 2011 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Jan 20 13:07:34 2011 Subject: [Histonet] H&E Stain In-Reply-To: Message-ID: <1206516859.1234623.1295550449538.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Allison/Toni, Thought I'd throw this out. Maybe nonsense. If you have such acidic tap water, could there be heavy metals lead, magnesium and others (acid tap water does that) in your tap water rinse from being leached out upstream. William DeSalvo talked about the quality of tap water fluctuating. Very true. And the metals from pipes or solder , leached into water by pH6.0, turning a normal hematoxylin into something like a Weigerts hematoxylin. A kind of post-mordanting that I think some call afterchroming. Although if you tried distilled or deionized water with same results, that data wouldn't fit with this problem. And even if it just started happening, has someone recently worked on pipes upsteam of where you are and there is (are) new metals being leached into your hematoxylin rinse? pH 6 is pretty acidic water. RayKoelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Toni Rathborne" To: "WILLIAM DESALVO" , jkiernan@uwo.ca, "allison scott" Cc: "histonet" Sent: Thursday, January 20, 2011 6:17:46 AM Subject: RE: [Histonet] H&E Stain Our tap water consistently reads 6.0, and has for years. We did try turning off the tap when this first began, and manually rinsing with distilled water, but saw no difference. I will try adding the HCl today with a few test slides. Will let you know how this works out. Thanks for the suggestions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of WILLIAM DESALVO Sent: Thursday, January 20, 2011 12:26 AM To: jkiernan@uwo.ca; allison_scott@hchd.tmc.edu Cc: histonet Subject: RE: [Histonet] H&E Stain I agree that the pH might be high, but I also suggest you check your water rinse on the stainer. If you are using "tap" water, there can be a significant fluctuation in the quality of the water and the amount of additives and impurities present at any one time can also contribute to the mucin not being rinsed away and staining. If you are using tap water, changing to distilled or dionized water might help to improve the consistency of stain results. Good luck William DeSalvo, B.S., HTL(ASCP) > From: jkiernan@uwo.ca > To: Allison_Scott@hchd.tmc.edu > Date: Wed, 19 Jan 2011 16:58:57 -0500 > Subject: Re: [Histonet] H&E Stain > CC: histonet@lists.utsouthwestern.edu > > Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. > > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = > ----- Original Message ----- > From: "Scott, Allison D" > Date: Wednesday, January 19, 2011 13:01 > Subject: [Histonet] H&E Stain > To: histonet@lists.utsouthwestern.edu > > > Hello to all in histoland and Happy New Year. We are > > having issues with > > our H&E stain. The nuclei are staining very blue to purple > > and the > > mucin is staining blue to purple-blue. It is difficult to > > see the > > nuclear detail. The mucin is obscuring things. We > > have not changed our > > process for staining or processing. The funny thing is > > that it is only > > in the Biopsy cases, and it is every few slides. The > > surgical cases > > are all right. We checked the alcohol and xylene for > > water, and there > > is not any. My tech changed out the stain and we are > > staining a new > > batch of slides. If anyone has any idea what is wrong, any > > help would > > be greatly appreciated. I have gone over our processes and > > nothing has > > changed. The reagents are the same, the staining times are > > the same, > > and the processing times are the same. We are using the > > Shandon Gemini > > stainer and VIP processor. > > > > Allison Scott HT(ASCP) > > Histology Supervisor > > LBJ Hospital > > Houston, Texas > > CONFIDENTIALITY NOTICE: > > If you have received this e-mail in error, please immediately > > notify the > > sender by return e-mail and delete this e-mail and any > > attachments from > > your computer system. > > > > To the extent the information in this e-mail and any attachments > > contain > > protected health information as defined by the Health Insurance > > Portability > > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > > Parts 160 and > > 164; or Chapter 181, Texas Health and Safety Code, it is > > confidential and/or > > privileged. This e-mail may also be confidential and/or > > privileged under > > Texas law. The e-mail is for the use of only the > > individual or entity named > > above. If you are not the intended recipient, or any > > authorized > > representative of the intended recipient, you are hereby > > notified that any > > review, dissemination or copying of this e-mail and its > > attachments is > > strictly prohibited. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 20 13:19:33 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 20 13:19:36 2011 Subject: [Histonet] Courier "Tracking Mechanism" In-Reply-To: <4D38276F.5D38.00EF.0@swmail.sw.org> Message-ID: <539078.5947.qm@web65704.mail.ac4.yahoo.com> Which is "the animal", the courier or the method? Ren? J. --- On Thu, 1/20/11, Nita Searcy wrote: From: Nita Searcy Subject: [Histonet] Courier "Tracking Mechanism" To: histonet@lists.utsouthwestern.edu Date: Thursday, January 20, 2011, 1:15 PM Am looking for a method for verification of specimens for couriers/ laboratories. Anyone use such an animal? Bill, as I recall you have in use such an animal? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Thu Jan 20 13:21:09 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Thu Jan 20 13:21:17 2011 Subject: [Histonet] NEW CAP? In-Reply-To: <4D380528.4347.0054.1@ah.org> References: <4D380528.4347.0054.1@ah.org> Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA768347@CMHEXCC01MBX.childrensmemorial.org> We have selected a standard antibody panel that we run and have the pathologist review when we get a new lot in. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Thursday, January 20, 2011 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NEW CAP? ANP.22760 NEW REAGENT LOT VERIFICATION New lots of antibody and detection system reagents are tested in parallel with old lots. Record of validation of new reagents/shipments I was wondering if you are using Ventana xt, How are you testing each time you use new DAB-kit ( new lot#) Thanks, Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Thu Jan 20 13:47:26 2011 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Jan 20 13:47:29 2011 Subject: [Histonet] Training Plan for Microtomy Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24669883@exchange.cmc-nh.org> I was wondering if any of you may have a microtomy training plan in your files that you would be willing to share? We are looking to expand some of the duties of some of our techs and would like to start things in motion by putting in place a good training plan prior to anyone getting near anything sharp. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From Barry.R.Rittman <@t> uth.tmc.edu Thu Jan 20 14:20:05 2011 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Jan 20 14:20:10 2011 Subject: [Histonet] RE: Training Plan for Microtomy In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC24669883@exchange.cmc-nh.org> References: <0908FC0A43B87A4FB60EDCCA06AABC24669883@exchange.cmc-nh.org> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9B6E60FBF@UTHCMS1.uthouston.edu> Stephen There are three excellent references. They all give much more than students need but provide nice context. "The Effective Use and Proper Care of the Microtome" Oscar W. Richards. Published by American Optical Company. Not sure when last published but my copy is 1959. This gives the theory and the history of microtomes and microtomy. The Microtome. A Guide to Specimen Preparation and Sectioning. Walter F. Published by the Leitz Company. Section Cutting in Microscopy. Steedman H.F. 1960 Blackwell Scientific Publications, Oxford. Great description of development of embedding media . Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Thursday, January 20, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training Plan for Microtomy I was wondering if any of you may have a microtomy training plan in your files that you would be willing to share? We are looking to expand some of the duties of some of our techs and would like to start things in motion by putting in place a good training plan prior to anyone getting near anything sharp. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Thu Jan 20 14:26:11 2011 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Jan 20 14:26:15 2011 Subject: [Histonet] RE: Training Plan for Microtomy In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC24669883@exchange.cmc-nh.org> References: <0908FC0A43B87A4FB60EDCCA06AABC24669883@exchange.cmc-nh.org> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9B6E60FC3@UTHCMS1.uthouston.edu> Stephen Sorry I forgot If you want a bare bones approach then Culling's book " Cellular Pathology Technique" has a good chapter on this. Published by Butterworths Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Thursday, January 20, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training Plan for Microtomy I was wondering if any of you may have a microtomy training plan in your files that you would be willing to share? We are looking to expand some of the duties of some of our techs and would like to start things in motion by putting in place a good training plan prior to anyone getting near anything sharp. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Thu Jan 20 14:47:01 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Thu Jan 20 14:47:05 2011 Subject: [Histonet] OHIO Position Message-ID: <6F33D8418806044682A391273399860F06A85C93@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamargasto.com. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From akemiat3377 <@t> yahoo.com Thu Jan 20 15:06:51 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Jan 20 15:06:54 2011 Subject: [Histonet] General supervisor requirement for CA and CLIA. Message-ID: <240914.7279.qm@web113819.mail.gq1.yahoo.com> Hi CA Histology Manager's, ? What is your understanding of who can supervisor the IHC department as a general supervisor?? Especially for licensed States like CA.? At Cedars?they had the IHC lab under the supervision of CLS tech and Managers.? I believe they ran into issues due to the high complexity testing issues.? My client will need to get on the same page on how?they are meeting the general supervisor requirement for CA and CLIA.? Thanks, Akemi Allison BS, HT(ASCP)HTL Director PhoenixLab Consulting E-Mail: akemiat3377@yahoo.com From jnocito <@t> satx.rr.com Thu Jan 20 18:31:20 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Jan 20 18:31:25 2011 Subject: [Histonet] Consult fees Message-ID: Just wanted to thank all of you for your responses. I may be a little sentimental and biased, but this is still a great forum. You all are great. I belong to another list server (which will left unnamed) and that "other" place doesn't even come close to the Histonet. Crap, I feel a tear or two coming on. Now I can't see the computer screen. Got to go and thanks again Joe From ccrowder <@t> vetmed.lsu.edu Thu Jan 20 20:01:01 2011 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Jan 20 20:03:54 2011 Subject: [Histonet] Animal tissue Message-ID: If you are processing mammalian tissue, it can be run with your regular "human' tissues. Rodent tissues are totally different and require shorter processing times. As long as your animal tissues are grossed at the proper thickness you should have no trouble. Cheryl From louise.renton <@t> gmail.com Fri Jan 21 01:31:59 2011 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Jan 21 01:32:04 2011 Subject: [Histonet] in situ hybridization Message-ID: Hi all, after a decade of not doing ISH, I have been asked to initiate a project in our department. needless to say i am VERY rusty. can any one suggest: - a good on-line resource to brush up my knowledge - a company that have reference materials/handbooks etc (I seem to recall Roche did this)? - A course/workshop that i can attend specifically on embryo and bone in situ? As always i thank the forum for its help best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From krishna_adhikari <@t> mail.com Fri Jan 21 04:05:49 2011 From: krishna_adhikari <@t> mail.com (krishna_adhikari@mail.com) Date: Fri Jan 21 04:13:19 2011 Subject: [Histonet] necropsy on freezed animal samples In-Reply-To: References: Message-ID: <8CD8769E9BDB9E4-140-5A12@web-mmc-d04.sysops.aol.com> Dear Group Members, I have a strange query, One person suggested, if the animals for necropsy are more and one can not handle the load of necropsy on the same day. Then simply after sacrificing the animals refrigerate the dead animals to reduce the autolysis process and then proceed with the actual necropsy procedures. is it possible to to the necropsy procedures on the freezed animals samples for further histopathology evaluation? expert comments are suggested. Regards krishna From Melissa.Kuhnla <@t> chsli.org Fri Jan 21 06:52:12 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri Jan 21 06:52:18 2011 Subject: [Histonet] NEW CAP? In-Reply-To: <4D380528.4347.0054.1@ah.org> References: <4D380528.4347.0054.1@ah.org> Message-ID: I am not a CAP inspected lab, but I do QC my ventana detection kits in two different ways. They are both very simple and manual. Both came to be because of faulty detections kits and actually events we experienced. 1. Load the detection kit on a wedge and manually dispense each dispenser onto a paper towel. Visually inspect the drops sizes. They all should be equal in size. Throughout the life of the dispenser I also randomly check visually the levels in each of the components. They should deplete at the same rate. 2. Dispense the first three dispensers in the same spot on the paper towel (the inhibitor, multimer, and chromogen). The wet make on the towel should turn brown proving that the chemical reaction will take place. I know it seems kinda silly, but this has actually saved us in the past from using a defective kit. I am curious to hear if anyone is actually slides. melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Thursday, January 20, 2011 12:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NEW CAP? ANP.22760 NEW REAGENT LOT VERIFICATION New lots of antibody and detection system reagents are tested in parallel with old lots. Record of validation of new reagents/shipments I was wondering if you are using Ventana xt, How are you testing each time you use new DAB-kit ( new lot#) Thanks, Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From akbitting <@t> geisinger.edu Fri Jan 21 08:01:24 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Jan 21 08:05:01 2011 Subject: [Histonet] Lab Assistants Embedding Message-ID: <4D394B63.2B7F.00C9.1@geisinger.edu> Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From Janice.Mahoney <@t> alegent.org Fri Jan 21 08:19:10 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Jan 21 08:19:19 2011 Subject: [Histonet] Lab Assistants Embedding In-Reply-To: <4D394B63.2B7F.00C9.1@geisinger.edu> References: <4D394B63.2B7F.00C9.1@geisinger.edu> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5355082@EXCHMBC2.ad.ah.local> As long as you are in a state that does not require licensure for Histo techs you are fine. Just be sure that you have training and competency performed and documented according to CAP. We have done this successfully in my lab. Jan, Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, January 21, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: [Histonet] Lab Assistants Embedding Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From trathborne <@t> somerset-healthcare.com Fri Jan 21 08:34:26 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jan 21 08:35:51 2011 Subject: [Histonet] Lab Assistants Embedding In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5355082@EXCHMBC2.ad.ah.local> Message-ID: If you are a CAP inspected lab, be sure to determine if your lab assistants qualify by meeting the guidelines in ANP11610. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, January 21, 2011 9:19 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding As long as you are in a state that does not require licensure for Histo techs you are fine. Just be sure that you have training and competency performed and documented according to CAP. We have done this successfully in my lab. Jan, Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, January 21, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: [Histonet] Lab Assistants Embedding Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From jeberry <@t> umich.edu Fri Jan 21 08:41:39 2011 From: jeberry <@t> umich.edu (Jan Berry) Date: Fri Jan 21 08:41:46 2011 Subject: [Histonet] Immunohistochemistry in plastic sections Message-ID: I'm wondering if anyone has experience with immunohistochemistry using plastic sections. I am currently working with 4um sections, and have tried doing staining with and without Dako antigen retrieval, but I am not getting very good results. Tissues (mostly mouse bones, but some soft tissues also) were fixed in PFA, cold embedded, and sections were deplastified using 3X 20 minutes methoxyethyl acetate and 2X 5minutes acetone, followed by 5 minutes in running dI water before beginning. I would appreciate any advice! Jan Berry, University of Michigan From mpence <@t> grhs.net Fri Jan 21 08:49:39 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jan 21 08:49:43 2011 Subject: [Histonet] Lab Assistants Embedding In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B0E@is-e2k3.grhs.net> Why, ANP11610 has to do with Gross Examination Qualifications for techs doing grossing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, January 21, 2011 8:34 AM To: Mahoney,Janice A; Angela Bitting; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding If you are a CAP inspected lab, be sure to determine if your lab assistants qualify by meeting the guidelines in ANP11610. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, January 21, 2011 9:19 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding As long as you are in a state that does not require licensure for Histo techs you are fine. Just be sure that you have training and competency performed and documented according to CAP. We have done this successfully in my lab. Jan, Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, January 21, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: [Histonet] Lab Assistants Embedding Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Dolores_Fischer <@t> baxter.com Fri Jan 21 08:50:36 2011 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Fri Jan 21 08:50:51 2011 Subject: [Histonet] necropsy on freezed animal samples In-Reply-To: <8CD8769E9BDB9E4-140-5A12@web-mmc-d04.sysops.aol.com> References: <8CD8769E9BDB9E4-140-5A12@web-mmc-d04.sysops.aol.com> Message-ID: Kirshna, I wouldn't consider this to be a good practice. I would expect that if the experimental study is important, thought and planning would have dictated that, at time of necropsy, enough resources would be available to properly cover the necropsy procedure. If an animal unexpectantly dies on study it is sometimes necessary to refrigerate it overnight if there is no personnel available to perform the necropsy (weekends or PM's). By refrigerating the animals and not getting the tissues samples in fixative ASAP you are compromising the tissue morphology. Your last sentence talks about freezed animals. Freezing would create freeze artifact, making the situation worse. Dolores -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of krishna_adhikari@mail.com Sent: Friday, January 21, 2011 4:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] necropsy on freezed animal samples Dear Group Members, I have a strange query, One person suggested, if the animals for necropsy are more and one can not handle the load of necropsy on the same day. Then simply after sacrificing the animals refrigerate the dead animals to reduce the autolysis process and then proceed with the actual necropsy procedures. is it possible to to the necropsy procedures on the freezed animals samples for further histopathology evaluation? expert comments are suggested. Regards krishna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From akbitting <@t> geisinger.edu Fri Jan 21 09:13:52 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Jan 21 09:14:04 2011 Subject: [Histonet] Lab Assistants Embedding In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974B0E@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974B0E@is-e2k3.grhs.net> Message-ID: <4D395C5F.2B7F.00C9.1@geisinger.edu> Yes, I wondered about that reference too. This is embedding, not grossing that I'm asking about. >>> "Mike Pence" 1/21/2011 9:49 AM >>> Why, ANP11610 has to do with Gross Examination Qualifications for techs doing grossing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, January 21, 2011 8:34 AM To: Mahoney,Janice A; Angela Bitting; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding If you are a CAP inspected lab, be sure to determine if your lab assistants qualify by meeting the guidelines in ANP11610. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, January 21, 2011 9:19 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding As long as you are in a state that does not require licensure for Histo techs you are fine. Just be sure that you have training and competency performed and documented according to CAP. We have done this successfully in my lab. Jan, Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, January 21, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: [Histonet] Lab Assistants Embedding Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From alisha <@t> ka-recruiting.com Fri Jan 21 09:32:02 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Fri Jan 21 09:32:15 2011 Subject: [Histonet] Laboratory Manager of Dermpath Lab in MI Message-ID: <688796499.1295623922381.JavaMail.cfservice@SL4APP4> Hi Histonet Members, I am currently working on a Laboratory Manager position in MIY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to alisha@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From mpence <@t> grhs.net Fri Jan 21 09:39:13 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jan 21 09:39:21 2011 Subject: [Histonet] Lab Assistants Embedding In-Reply-To: <4D395C5F.2B7F.00C9.1@geisinger.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B10@is-e2k3.grhs.net> As long as you document guidelines, training and continued competencies you will be okay. -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, January 21, 2011 9:14 AM To: Janice A Mahoney; Mike Pence; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu; Toni Rathborne Subject: RE: [Histonet] Lab Assistants Embedding Yes, I wondered about that reference too. This is embedding, not grossing that I'm asking about. >>> "Mike Pence" 1/21/2011 9:49 AM >>> Why, ANP11610 has to do with Gross Examination Qualifications for techs doing grossing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, January 21, 2011 8:34 AM To: Mahoney,Janice A; Angela Bitting; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding If you are a CAP inspected lab, be sure to determine if your lab assistants qualify by meeting the guidelines in ANP11610. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, January 21, 2011 9:19 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding As long as you are in a state that does not require licensure for Histo techs you are fine. Just be sure that you have training and competency performed and documented according to CAP. We have done this successfully in my lab. Jan, Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, January 21, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: [Histonet] Lab Assistants Embedding Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From mhale <@t> carisls.com Fri Jan 21 09:42:41 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri Jan 21 09:42:48 2011 Subject: [Histonet] OHIO Position Message-ID: <6F33D8418806044682A391273399860F06AD727A@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamargasto.com. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From judi <@t> medialabinc.net Fri Jan 21 09:52:50 2011 From: judi <@t> medialabinc.net (Judi Bennett) Date: Fri Jan 21 09:52:56 2011 Subject: [Histonet] MediaLab is Looking for Histology Course Authors! Message-ID: Actively seeking authors to write and review online *histology courses* for *MediaLab*! MediaLab is a leading publisher of online continuing education (CE) courses and competency assessments. Our online products are used at more than 2,000 laboratories and university CLS programs worldwide. This is a great opportunity to *gain resume-boosting publishing experience, **earn honorariums* for your participation, and fill the *need to provide quality histology CE credits*. Authors can take advantage of MediaLab's online CourseBuilder to *write courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive interface similar to Microsoft Word or PowerPoint. Authors can quickly create content pages, practice questions, and exam questions, and upload relevant images. Courses developed by MediaLab are *featured on our websites MediaLabInc.net and LabCE.com*. Questions from these courses also become part of the LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers may also *contribute to other online programs that we develop on behalf of major laboratory partners*. To learn more about becoming a MediaLab author for histology courses, visit our online information page at www.medialbinc.net/authors.aspx . Please contact Judi Bennett at judi@medialabinc.net or call 877-776-8460, ext. 721. Judi Bennett, BSM, MT(AMT) MediaLab, Inc. e-mail judi@medialabinc.net Phone (877) 776-8460 ext. 721 cell phone 404-915-2999 fax (678) 401-0284 From judi <@t> medialabinc.net Fri Jan 21 10:12:26 2011 From: judi <@t> medialabinc.net (Judi Bennett) Date: Fri Jan 21 10:12:33 2011 Subject: [Histonet] Correction to MediaLab Author Link Message-ID: My apologies! Please use this corrected link to learn more about becoming a MediaLab author for histology courses, visit our online information page at www.medialabinc.net/authors.aspx . Please contact Judi Bennett at judi@medialabinc.net or call 877-776-8460, ext. 721. -- Judi Bennett, BSM, MT(AMT) MediaLab, Inc. e-mail judi@medialabinc.net Phone (877) 776-8460 ext. 721 cell phone 404-915-2999 fax (678) 401-0284 From mcauliff <@t> umdnj.edu Fri Jan 21 10:21:14 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jan 21 10:18:14 2011 Subject: [Histonet] necropsy on freezed animal samples In-Reply-To: <8CD8769E9BDB9E4-140-5A12@web-mmc-d04.sysops.aol.com> References: <8CD8769E9BDB9E4-140-5A12@web-mmc-d04.sysops.aol.com> Message-ID: <4D39B27A.3020400@umdnj.edu> If the tissue is not fixed soon after death the microscopic morphology will be terrible, no matter whether the animal is refrigerated or frozen. The person suggesting refrigeration/freezing is (obviously) not familiar with histological technique. As someone else on the list suggested, better planning is needed for the harvesting of tissues for microscopy. Geoff On 1/21/2011 5:05 AM, krishna_adhikari@mail.com wrote: > > Dear Group Members, > I have a strange query, One person suggested, if the animals for necropsy are more and one can not handle the load of necropsy on the same day. > Then simply after sacrificing the animals refrigerate the dead animals to reduce the autolysis process and then proceed with the actual necropsy procedures. > is it possible to to the necropsy procedures on the freezed animals samples for further histopathology evaluation? > expert comments are suggested. > Regards > krishna > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Jan 21 11:21:58 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Jan 21 11:22:03 2011 Subject: [Histonet] C3d In-Reply-To: <4D3829F4.7400.0077.1@harthosp.org> References: <4D3829F4.7400.0077.1@harthosp.org> Message-ID: Dr. Cartun, We are using the C3d and C4d from Cell Marque. On the Dako platform. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Thursday, January 20, 2011 12:26 PM To: Histonet Subject: [Histonet] C3d Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anonwums1 <@t> gmail.com Fri Jan 21 11:57:28 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Jan 21 11:57:31 2011 Subject: [Histonet] necropsy on freezed animal samples In-Reply-To: <4D39B27A.3020400@umdnj.edu> References: <8CD8769E9BDB9E4-140-5A12@web-mmc-d04.sysops.aol.com> <4D39B27A.3020400@umdnj.edu> Message-ID: I recently had an animal from a previously undescribed mouse model die suddenly during the weekend. Since I couldn't send it off to necropsy, I stashed it in the refrigerator until Monday morning. According to the person who read the histology "Post-mortem degeneration limited evaluation of most tissues." So I would agree from experience that this is not a good idea. Adam On Fri, Jan 21, 2011 at 10:21 AM, Geoff McAuliffe wrote: > If the tissue is not fixed soon after death the microscopic morphology will > be terrible, no matter whether the animal is refrigerated or frozen. The > person suggesting refrigeration/freezing is (obviously) not familiar with > histological technique. > As someone else on the list suggested, better planning is needed for the > harvesting of tissues for microscopy. > > Geoff > > > On 1/21/2011 5:05 AM, krishna_adhikari@mail.com wrote: > >> >> Dear Group Members, >> I have a strange query, One person suggested, if the animals for necropsy >> are more and one can not handle the load of necropsy on the same day. >> Then simply after sacrificing the animals refrigerate the dead animals to >> reduce the autolysis process and then proceed with the actual necropsy >> procedures. >> is it possible to to the necropsy procedures on the freezed animals >> samples for further histopathology evaluation? >> expert comments are suggested. >> Regards >> krishna >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jm.lapointe <@t> accellab.com Fri Jan 21 12:21:58 2011 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Fri Jan 21 12:22:02 2011 Subject: [Histonet] Immunohistochemistry in plastic sections In-Reply-To: <201101211804.p0LI4IeK030239@gateway8.lastspam.com> References: <201101211804.p0LI4IeK030239@gateway8.lastspam.com> Message-ID: Hi Jan IHC on plastic-embedded specimens is difficult, I think the antigens can get rather damaged from the embedding and/or deplastifying processes. In our experience, it's possible to do some markers, while others are irretrievable. One thing I notice is that our protocol for deplastification calls for 2x MEA 20 minutes, while yours does 3x. Perhaps cutting down to 2x would be sufficient (your sections are thinner than ours, too), and would potentially cause less damage. Good luck __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc ? ------------------------------ Message: 4 Date: Fri, 21 Jan 2011 09:41:39 -0500 From: Jan Berry Subject: [Histonet] Immunohistochemistry in plastic sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii I'm wondering if anyone has experience with immunohistochemistry using plastic sections. I am currently working with 4um sections, and have tried doing staining with and without Dako antigen retrieval, but I am not getting very good results. Tissues (mostly mouse bones, but some soft tissues also) were fixed in PFA, cold embedded, and sections were deplastified using 3X 20 minutes methoxyethyl acetate and 2X 5minutes acetone, followed by 5 minutes in running dI water before beginning. I would appreciate any advice! Jan Berry, University of Michigan ************************************** From TNMayer <@t> mdanderson.org Fri Jan 21 13:10:11 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Fri Jan 21 13:10:15 2011 Subject: [Histonet] RE: Contents of Histonet Digest, Vol 86, Issue 28 In-Reply-To: <6779d9f2-46e0-48c2-8c8e-262725968085@DCPWPRTR03.mdanderson.edu> References: <6779d9f2-46e0-48c2-8c8e-262725968085@DCPWPRTR03.mdanderson.edu> Message-ID: I have worked in several labs where lab assistants were embedding. They were even doing it in the midst of CAP inspection. It was not an issue. Proper training documentation was done and each block was cut by an experienced tech. As a matter of fact, in one lab it was so basic that symbols were implemented and used to identify embedding instructions for the assistants. In one lab, the one of the assistants is better than some of the techs at embedding. It worked out better for the workflow, because the techs were freed up to cut. Anything that was questionable was taken care of by a tech as well. All of the labs I am referring to are in Texas. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- Message: 1 Date: Fri, 21 Jan 2011 09:01:24 -0500 From: "Angela Bitting" Subject: [Histonet] Lab Assistants Embedding To: , Message-ID: <4D394B63.2B7F.00C9.1@geisinger.edu> Content-Type: text/plain; charset="us-ascii" Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 ****************** From kblack <@t> digestivehlth.com Fri Jan 21 13:18:30 2011 From: kblack <@t> digestivehlth.com (Konni Black) Date: Fri Jan 21 13:18:38 2011 Subject: [Histonet] part-time HT/HTL position in Scottsdale AZ Message-ID: <67BA2079E0A342158974A85C12B22CB0@digestivehlth.com> Hi All, There is a part-time opening in a great new lab in sunny Scottsdale, AZ.. Licensed HT or HTL must have 3 or more years experience and be able to work independently. Great pay, very flexible schedule. Please contact Konni Black for more details. Thank you for your interest. 253-503-2560 office 253-312-9964 cell 253-682-2433 fx From adschult <@t> travmax.com Fri Jan 21 13:48:41 2011 From: adschult <@t> travmax.com (Adam Schultz) Date: Fri Jan 21 13:48:49 2011 Subject: [Histonet] Contract Histo Tech Needs in South Carolina Message-ID: <3E6BB83FECC1E64197E1E58ED9208C16088787AA9F@EXCLUSTER01.maxhealth.com> Hello Everyone!! I am looking for Two Histotechnologists to perform various routine duties and/or highly complex analysis (immunoperoxidase and cutting tissue) within the Histology Section of Pathology and Laboratory Medicine at a hospital in Columbia, SC. Work hours are 8a-4:30pm M-F, no weekends, no holidays. Contract will go for 17 weeks in length with possibility of extension. Please contact Adam Schultz @ (813) 371-3427 or Kevin Lucania @ (813) 371-5174. They can both also be reached toll free at 888-800-1855. ***Note - Minimum two years experience required to apply*** Thank you, Adam Schultz TravelMax Medical Professionals 813-371-3427 adschult@travmax.com ________________________________ Confidentiality Statement: The information contained in this facsimile/email transmission is privileged and confidential and is intended only for the use of the recipient listed above. If you are neither the intended recipient or an employee or agent of the intended recipient responsible for the delivery of this information, you are hereby notified that the disclosure, copying, use or distribution of this information is strictly prohibited. If you have received this transmission in error, please notify us immediately to arrange for the return of the transmitted documents or to verify their destruction. From laurie.colbert <@t> huntingtonhospital.com Fri Jan 21 14:48:58 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Jan 21 14:49:02 2011 Subject: [Histonet] Storing Acetic Acid Message-ID: <57BE698966D5C54EAE8612E8941D76830A698D70@EXCHANGE3.huntingtonhospital.com> I am trying to find out how to store acetic acid. We were storing it in our acid cabinet with the other acids, but we were inspected by the fire department last week and they said that acetic acid is flammable and a corrosive and that it should be stored with the flammables. But my manager is saying that you can't store corrosives and alcohols together, so I'm not sure where it should actually be stored (other than in some cabinet all by itself)! Laurie Colbert From rjbuesa <@t> yahoo.com Fri Jan 21 14:57:53 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 21 14:57:58 2011 Subject: [Histonet] Storing Acetic Acid In-Reply-To: <57BE698966D5C54EAE8612E8941D76830A698D70@EXCHANGE3.huntingtonhospital.com> Message-ID: <753059.18325.qm@web65714.mail.ac4.yahoo.com> Check the MSDS, they should contain storage guidelines. Just follow them Ren? J. --- On Fri, 1/21/11, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Storing Acetic Acid To: histonet@lists.utsouthwestern.edu Date: Friday, January 21, 2011, 3:48 PM I am trying to find out how to store acetic acid.? We were storing it in our acid cabinet with the other acids, but we were inspected by the fire department last week and they said that acetic acid is flammable and a corrosive and that it should be stored with the flammables.? But my manager is saying that you can't store corrosives and alcohols together, so I'm not sure where it should actually be stored (other than in some cabinet all by itself)! Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Jan 21 15:01:09 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Jan 21 15:01:26 2011 Subject: [Histonet] Lab Assistants Embedding In-Reply-To: <4D395C5F.2B7F.00C9.1@geisinger.edu> References: <661949901A768E4F9CC16D8AF8F2838C03974B0E@is-e2k3.grhs.net> <4D395C5F.2B7F.00C9.1@geisinger.edu> Message-ID: Hi Angie, Jan M has nailed it. Have a good weekend. - William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, January 21, 2011 9:14 AM To: Janice A Mahoney; Mike Pence; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu; Toni Rathborne Subject: RE: [Histonet] Lab Assistants Embedding Yes, I wondered about that reference too. This is embedding, not grossing that I'm asking about. >>> "Mike Pence" 1/21/2011 9:49 AM >>> Why, ANP11610 has to do with Gross Examination Qualifications for techs doing grossing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, January 21, 2011 8:34 AM To: Mahoney,Janice A; Angela Bitting; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding If you are a CAP inspected lab, be sure to determine if your lab assistants qualify by meeting the guidelines in ANP11610. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, January 21, 2011 9:19 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding As long as you are in a state that does not require licensure for Histo techs you are fine. Just be sure that you have training and competency performed and documented according to CAP. We have done this successfully in my lab. Jan, Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, January 21, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: [Histonet] Lab Assistants Embedding Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. 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Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Fri Jan 21 15:34:15 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Jan 21 15:34:19 2011 Subject: [Histonet] billing Message-ID: for those of you that are doing her2 immunos, are you charging more for these than for other antibodies? I pay way more for these, and it seems we should be able to charge more? thanks, everyone have a great weekend!! anita dudley providence hospital mobile, alabama From gayle.callis <@t> bresnan.net Fri Jan 21 17:56:52 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jan 21 17:57:02 2011 Subject: [Histonet] RE: IHC on plastic sections Message-ID: <000101cbb9c6$e242e890$a6c8b9b0$@callis@bresnan.net> You did not say WHICH plastic? Immunostaining on tissues embedded in methyl methacrylate are successful since one can totally remove this plastic from the tissue with various solvents. There may be some stringent antigen retrieval needed . Neil Hand had a panel of over 200 antibodies with successful staining of tissue embedded in MMA, and retrieval with a HIER pressure cooker system, a published method in Journal of Histotechnology. Neil Hand has written a chapter on plastics and how to do the IHC in Gamble and Bancroft's Theory and Practice of Histological Techniques, both 5th and 6th editions. If the plastic is glycol methacrylate, then it is less successful to no staining, since this plastic, once polymerized, cannot be removed or dissolved away with a solvent. The large immunoglobulins (antibodies) cannot penetrate the plastic matrix to get to antigen very well. There are publications where people has some success with GMA but most people don't succeed. EM resins can be etched with sodium ethoxide, and other chemical methods, but there are attendant problems with these methods. Gayle M. Callis HTL/HT/MT()ASCP) Bozeman MT From adesupo2002 <@t> hotmail.com Sat Jan 22 19:33:09 2011 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Sat Jan 22 19:34:41 2011 Subject: [Histonet] Laboratory Safety, SLS(ASCP). Message-ID: Hi, Please I am wondering if you guys here in histoland could recommend some reading materials for the ASCP Board of Certification (BOC) Examination in Laboratory Safety, SLS(ASCP). You guys are the best. B . Adesuyi , BS, HT(ASCP)HTL, QIHC(ASCP) From rsrichmond <@t> gmail.com Sat Jan 22 21:25:21 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Jan 22 21:25:27 2011 Subject: [Histonet] Re: Storing acetic acid Message-ID: Laurie Colbert (where?) asks: > I am trying to find out how to store acetic acid. We were storing it in > our acid cabinet with the other acids, but we were inspected by the fire > department last week and they said that acetic acid is flammable and a > corrosive and that it should be stored with the flammables. But my > manager is saying that you can't store corrosives and alcohols together, > so I'm not sure where it should actually be stored (other than in some > cabinet all by itself)! The Fisher Scientific MSDS - at http://fscimage.fishersci.com/msds/00120.htm advises: Storage: Keep away from heat, sparks, and flame. Keep from contact with oxidizing materials. Store in a cool, dry, well-ventilated area away from incompatible substances. Do not store near alkaline substances. Acetic acid should be kept above its freezing point of 62 degrees F (17 degrees C) to allow it to be handled as a liquid. It will contract slightly on freezing. Freezing and thawing does not affect product quality. Bob Richmond Samurai Pathologist Knoxville TN From tkngflght <@t> yahoo.com Sun Jan 23 12:09:47 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sun Jan 23 12:09:51 2011 Subject: [Histonet] acetic acid Message-ID: <556527.66097.qm@web39409.mail.mud.yahoo.com> ?Richard- ? If it is a small amount (>1L) you may be able to store it in your fume hood assuming you've no contradictory chemicals stored there at the same time.??? Best to keep it in an isolation container like the foam insert it probably came in. ? If you use a dilute concentration on the bench, next time purchase it in the appropriate dilution rather than as a pure form, and the water in the mix takes it out of the flammable range and then it can go with your other acids. ? Hope this helps! ? Cheryl Full Staff Inc. From rsrichmond <@t> gmail.com Sun Jan 23 12:21:47 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sun Jan 23 12:21:57 2011 Subject: [Histonet] Re: Laboratory Safety, SLS(ASCP). Message-ID: From: ADESUPO ADESUYI Subject: [Histonet] Laboratory Safety, SLS(ASCP). B . Adesuyi , BS, HT(ASCP)HTL, QIHC(ASCP) asks: >>Please I am wondering if you guys here in histoland could recommend some reading materials for the ASCP Board of Certification (BOC) Examination in Laboratory Safety, SLS(ASCP).<< When you study for an exam, you have to find out what study materials you're supposed to study. Someone on Histonet will know the answer to this question. When I actually have a problem, I consult the Dapsons' book Hazardous Materials in the Histopathology Laboratory, available from Anatech. (I have no connection with Anatech.) See www.anatechltdusa.com Bob Richmond Samurai Pathologist Knoxville TN From kdwyer3322 <@t> aol.com Sun Jan 23 17:10:56 2011 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Jan 23 17:11:08 2011 Subject: [Histonet] Texas Society for Histotechnology 2011 Symposium Convention Message-ID: <8CD8969ECBB4ED9-EF0-1B963@webmail-d062.sysops.aol.com> All, The TSH Symposium Convention will be held at the Marriott Legacy Town Center in Plano, Texas (DFW area) March 31- April 3, 2011. There is still time to register for the meeting and reserve a room at the hotel. If you would like a copy of the program please contact kdwyer3322@aol.com. TSH Convention Committee From lpwenk <@t> sbcglobal.net Sun Jan 23 17:51:37 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Jan 23 18:35:10 2011 Subject: [Histonet] Whole mount histology In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43FD0CF2993F@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43FD0CF2993F@HPEMX3.HealthPartners.int> Message-ID: Don't know how fast you need this information, but there will be a NSH teleconference on this topic on May 25, 2011. www.nsh.org 443-535-4060 Peggy A. Wenk. HTL(ASCP)SLS NSH Teleconference coordinator (Disclaimer: volunteer position, so don't get paid if anyone signs up) -------------------------------------------------- From: "Webb, Dorothy L" Sent: Monday, January 17, 2011 10:51 AM To: Subject: [Histonet] Whole mount histology > I am totally unfamiliar regarding whole mount histology as it pertains to > prostate histology. Can anyone in histoland assist me in finding > information out regarding more specifics, especially in lieu of what costs > would be incurred to implement this in a routine hospital histology > department. > > Appreciate any and all guidance!! > > Dorothy Webb, HT > Regions Histology Technical Specialist 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Mon Jan 24 01:31:20 2011 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Jan 24 01:31:31 2011 Subject: [Histonet] in situ methodology part 2 Message-ID: Hi all just as clarification .....i need to brush up on my background of ISH - what new products/methododolgies there might be on the market. The tissues I will be using will either be rodent pups/embryos or formalin fixed/fresh adult tissue. As for the probe - thats a stick point - not enough background information to decide - but probably oligos. So...the questions still stand: - where can i find good on-line material - Who offers the best application manuals - is Roche still int he market? best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From cpyse <@t> x-celllab.com Mon Jan 24 06:57:30 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Mon Jan 24 06:57:53 2011 Subject: [Histonet] ihc slides Message-ID: <000c01cbbbc6$429682f0$c7c388d0$@com> Happy Monday Histonetters I need to pick the brains of the IHC experts out there in histoland. I run IHC on the Dako autostainer. On one of our runs there was not enough DAB to dispense on all the slides. My tech dehydrated the slides and cover slipped them. We printed the stain log on the run and confirmed that the DAB was not dispensed on three slides. The cover slips were removed and the slide were soaked in xylene to remove the cover slipping media. The slides were rehydrated and DAB was applied. There was still no reaction after 10 minutes, The DAB was reapplied for up to 30 minutes with no reaction. Can anyone out there give me a reason that the slides would not react after the reapplication of the DAB. I have done this before when the DAB solution was short on the run and it worked. I can't figure out why this didn't react. I called tech service at Dako and talked to the supervisor, she agrees with me that the reapplication should have worked. I checked the run log and the only missing step is the DAB. The antibodies with the missing DAB have their own controls on the slides. Any thought out there as to what the problem is. I have had the PM done on the Dako which should resolve the problem according to Dako. There has not been a problem since. This mystery will drive me crazy until I can figure out why. I could use help. Thanks in advance, I appreciate any input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com From MSHERWOOD <@t> PARTNERS.ORG Mon Jan 24 08:48:05 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Jan 24 08:48:10 2011 Subject: [Histonet] acetic acid In-Reply-To: <556527.66097.qm@web39409.mail.mud.yahoo.com> References: <556527.66097.qm@web39409.mail.mud.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB53D7@PHSXMB30.partners.org> We have always kept our acetic acid under our hood with our other acids. We do keep it in the styrofoam container with which it is mailed. We've never had a problem. We only keep about 500ml on hand at any one time. (We are a research lab, so maybe histology labs need to have more). Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Sunday, January 23, 2011 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] acetic acid ?Richard- ? If it is a small amount (>1L) you may be able to store it in your fume hood assuming you've no contradictory chemicals stored there at the same time.??? Best to keep it in an isolation container like the foam insert it probably came in. ? If you use a dilute concentration on the bench, next time purchase it in the appropriate dilution rather than as a pure form, and the water in the mix takes it out of the flammable range and then it can go with your other acids. ? Hope this helps! ? Cheryl Full Staff Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From TGoins <@t> mt.gov Mon Jan 24 09:40:43 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Jan 24 09:40:48 2011 Subject: [Histonet] ihc slides In-Reply-To: <000c01cbbbc6$429682f0$c7c388d0$@com> References: <000c01cbbbc6$429682f0$c7c388d0$@com> Message-ID: Cindy - I think you got some bad information from Dako tech service. The buffers used during IHC not only keep the tissue hydrated but provide an environment [salts, pH] that promotes strong binding between the antibody and antigen target. The antigen-antibody bond is not a covalent chemical bond and will not be maintained during coverslip removal. So don't go crazy, the slides were shot. Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Monday, January 24, 2011 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ihc slides Happy Monday Histonetters I need to pick the brains of the IHC experts out there in histoland. I run IHC on the Dako autostainer. On one of our runs there was not enough DAB to dispense on all the slides. My tech dehydrated the slides and cover slipped them. We printed the stain log on the run and confirmed that the DAB was not dispensed on three slides. The cover slips were removed and the slide were soaked in xylene to remove the cover slipping media. The slides were rehydrated and DAB was applied. There was still no reaction after 10 minutes, The DAB was reapplied for up to 30 minutes with no reaction. Can anyone out there give me a reason that the slides would not react after the reapplication of the DAB. I have done this before when the DAB solution was short on the run and it worked. I can't figure out why this didn't react. I called tech service at Dako and talked to the supervisor, she agrees with me that the reapplication should have worked. I checked the run log and the only missing step is the DAB. The antibodies with the missing DAB have their own controls on the slides. Any thought out there as to what the problem is. I have had the PM done on the Dako which should resolve the problem according to Dako. There has not been a problem since. This mystery will drive me crazy until I can figure out why. I could use help. Thanks in advance, I appreciate any input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Mon Jan 24 10:00:17 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Mon Jan 24 10:00:25 2011 Subject: [Histonet] ihc slides In-Reply-To: <000c01cbbbc6$429682f0$c7c388d0$@com> References: <000c01cbbbc6$429682f0$c7c388d0$@com> Message-ID: Hi Cindy, That too has happened to me and the process that you do to remedy it, works for me too. (take off coverslip properly, re-apply DAB, and you get a reaction.) My experience is that the slides are not "shot", as described by another Histonetter. There was definitely something else going on. I work with the Dako too. Is it possible that the Autostainer was not programmed to apply antibody in the crucial zones? Look into that possibility. Good Luck. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Monday, January 24, 2011 7:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ihc slides Happy Monday Histonetters I need to pick the brains of the IHC experts out there in histoland. I run IHC on the Dako autostainer. On one of our runs there was not enough DAB to dispense on all the slides. My tech dehydrated the slides and cover slipped them. We printed the stain log on the run and confirmed that the DAB was not dispensed on three slides. The cover slips were removed and the slide were soaked in xylene to remove the cover slipping media. The slides were rehydrated and DAB was applied. There was still no reaction after 10 minutes, The DAB was reapplied for up to 30 minutes with no reaction. Can anyone out there give me a reason that the slides would not react after the reapplication of the DAB. I have done this before when the DAB solution was short on the run and it worked. I can't figure out why this didn't react. I called tech service at Dako and talked to the supervisor, she agrees with me that the reapplication should have worked. I checked the run log and the only missing step is the DAB. The antibodies with the missing DAB have their own controls on the slides. Any thought out there as to what the problem is. I have had the PM done on the Dako which should resolve the problem according to Dako. There has not been a problem since. This mystery will drive me crazy until I can figure out why. I could use help. Thanks in advance, I appreciate any input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Mon Jan 24 10:31:04 2011 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Jan 24 10:31:13 2011 Subject: [Histonet] ihc slides In-Reply-To: <000c01cbbbc6$429682f0$c7c388d0$@com> References: <000c01cbbbc6$429682f0$c7c388d0$@com> Message-ID: <4D3D7108020000C80000DB85@smtp1.gov.pe.ca> Cynthia, Hematoxylin will inactivate the peroxidase (link) in the tissues. You must go back and re-do the link step and hopefully the antigenic sites will still accept more streptavidin (or similar) conjugated antibody. Cheer. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Cynthia Pyse" 1/24/2011 8:57 AM >>> Happy Monday Histonetters I need to pick the brains of the IHC experts out there in histoland. I run IHC on the Dako autostainer. On one of our runs there was not enough DAB to dispense on all the slides. My tech dehydrated the slides and cover slipped them. We printed the stain log on the run and confirmed that the DAB was not dispensed on three slides. The cover slips were removed and the slide were soaked in xylene to remove the cover slipping media. The slides were rehydrated and DAB was applied. There was still no reaction after 10 minutes, The DAB was reapplied for up to 30 minutes with no reaction. Can anyone out there give me a reason that the slides would not react after the reapplication of the DAB. I have done this before when the DAB solution was short on the run and it worked. I can't figure out why this didn't react. I called tech service at Dako and talked to the supervisor, she agrees with me that the reapplication should have worked. I checked the run log and the only missing step is the DAB. The antibodies with the missing DAB have their own controls on the slides. Any thought out there as to what the problem is. I have had the PM done on the Dako which should resolve the problem according to Dako. There has not been a problem since. This mystery will drive me crazy until I can figure out why. I could use help. Thanks in advance, I appreciate any input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From gvdobbin <@t> ihis.org Mon Jan 24 10:34:30 2011 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Jan 24 10:34:38 2011 Subject: [Histonet] ihc slides In-Reply-To: <000c01cbbbc6$429682f0$c7c388d0$@com> References: <000c01cbbbc6$429682f0$c7c388d0$@com> Message-ID: <4D3D71D6020000C80000DB8B@smtp1.gov.pe.ca> I should add that the DAB step may also have "quenched" all of the linked peroxidases even before the hematoxilin was added (which would finish off any that remained). Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Cynthia Pyse" 1/24/2011 8:57 AM >>> Happy Monday Histonetters I need to pick the brains of the IHC experts out there in histoland. I run IHC on the Dako autostainer. On one of our runs there was not enough DAB to dispense on all the slides. My tech dehydrated the slides and cover slipped them. We printed the stain log on the run and confirmed that the DAB was not dispensed on three slides. The cover slips were removed and the slide were soaked in xylene to remove the cover slipping media. The slides were rehydrated and DAB was applied. There was still no reaction after 10 minutes, The DAB was reapplied for up to 30 minutes with no reaction. Can anyone out there give me a reason that the slides would not react after the reapplication of the DAB. I have done this before when the DAB solution was short on the run and it worked. I can't figure out why this didn't react. I called tech service at Dako and talked to the supervisor, she agrees with me that the reapplication should have worked. I checked the run log and the only missing step is the DAB. The antibodies with the missing DAB have their own controls on the slides. Any thought out there as to what the problem is. I have had the PM done on the Dako which should resolve the problem according to Dako. There has not been a problem since. This mystery will drive me crazy until I can figure out why. I could use help. Thanks in advance, I appreciate any input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From pruegg <@t> ihctech.net Mon Jan 24 11:05:00 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Jan 24 11:05:41 2011 Subject: SPAM-LOW: [Histonet] in situ methodology part 2 In-Reply-To: References: Message-ID: <2BCED9C0BCC9491193AD5C3747B0C55C@Patsyoffice> Louise, I have been testing a new ISH kit called RISH from BioCare. It works on any dig labeled mRna probe. The method is designed for ffpe sections but I have just adapted it to cryosections as well. I can send you some pictures of Tunnel and EBV on ffpe sections if you would like to see them. Besides Roche this is the only kit I know of out right now for ISH. They sell the kit with their probes, I think they only have EBV, Kappa and Lambda and maybe CMV. They say they are coming out with hpv probes this spring but I have not seen them yet. They will also sell the reagents without their probe for researchers like you and me that use our own probes as long as they are dig labeled. Ventana has ISH for HPV but the probe is fitc labeled. In research we prefer dig labeled probes, at least my investigators do. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, January 24, 2011 12:31 AM To: Histonet Subject: SPAM-LOW: [Histonet] in situ methodology part 2 Hi all just as clarification .....i need to brush up on my background of ISH - what new products/methododolgies there might be on the market. The tissues I will be using will either be rodent pups/embryos or formalin fixed/fresh adult tissue. As for the probe - thats a stick point - not enough background information to decide - but probably oligos. So...the questions still stand: - where can i find good on-line material - Who offers the best application manuals - is Roche still int he market? best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heidi.Hawthorne <@t> onassignment.com Mon Jan 24 12:51:39 2011 From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne) Date: Mon Jan 24 12:51:45 2011 Subject: [Histonet] Histotech Needed Martinez, CA ASAP! Message-ID: <26C4A3B38503BC4CBBF4D986C3BC9B83087EE0D7DE@oasslcexm01.oaifield.onasgn.com> Histotechnician Needed - Martinez, CA We are currently seeking experienced Histotechnician to work in a full-time contract position in Martinez, CA. Monday through Friday 6am- 2:30pm. Great opportunity! Histotech needed to prepare tissue specimens for examination; dehydrates, blocks, cuts, stains and mounts tissue specimens; and performs related work as required. Applicants must have at least 6 months experience in a histology lab. For immediate consideration, please email resume to: heidi.hawthorne@onassignment.com. From kmerriam2003 <@t> yahoo.com Mon Jan 24 13:52:46 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Jan 24 13:52:49 2011 Subject: [Histonet] fibroblast marker in mouse tissue Message-ID: <491189.28693.qm@web130101.mail.mud.yahoo.com> Hi All, What is everyone using?to stain for fibroblasts in frozen mouse tissue?? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From sfeher <@t> CMC-NH.ORG Mon Jan 24 13:53:14 2011 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Jan 24 13:53:18 2011 Subject: [Histonet] Lab Assistants Embedding In-Reply-To: <4D394B63.2B7F.00C9.1@geisinger.edu> References: <4D394B63.2B7F.00C9.1@geisinger.edu> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC246698D1@exchange.cmc-nh.org> I attended a seminar recently that was given by an Pathologist who was an experienced "expert witness". The substance of the seminar addressed the top items that would be looked at when a pathology lab is involved in a lawsuit. One item that was specifically mentioned was that tissue orientation within the block is often examined as a potential reason for false negative surgical cases. The statistics mentioned were all for derm specimens and how when the block was sectioned through, it was reasoned that tissue orientation within the block was at fault. So, the sound advice given by all of you to document and train properly is vital. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, January 21, 2011 9:01 AM To: histonet@lists.utsouthwestern.edu; Histonet-requests@lists.utsouthwestern.edu Subject: [Histonet] Lab Assistants Embedding Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From NMargaryan <@t> childrensmemorial.org Mon Jan 24 15:37:31 2011 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Jan 24 15:39:48 2011 Subject: [Histonet] ihc slides In-Reply-To: References: Message-ID: Hi Cynthia, I agree with Greg, you have to re-do your staining from beginning (from AR step) after removing the hematoxilin. Naira From doakes <@t> olympicmedical.org Mon Jan 24 16:46:35 2011 From: doakes <@t> olympicmedical.org (Dawn Oakes) Date: Mon Jan 24 16:46:39 2011 Subject: [Histonet] Mohs Message-ID: I'm in need of some info from Mohs Techs. Average time to cut 1 patient? How many patients a day? Anybody cut or get multiple patients at the same time? I would love to hear from you ! Thank You ,Dawn Dawn Oakes HT Olympic Medical Center ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From foreightl <@t> gmail.com Mon Jan 24 16:56:57 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Mon Jan 24 16:57:03 2011 Subject: [Histonet] Re: Banana smelling chemical in our formalin In-Reply-To: References: Message-ID: > Histonet, > > We get a number of our specimens from outreach clients. We received back > from one of our sites a jug of "Formalin" to be recycled. However when it > was opened up it had a strong odor of bananas. I know we shouldn't smell > the chemicals, but luckily this was caught before it was put through our > recycler. I was wondering if anyone might have an idea what this could be? > Is there a manufacturer that adds an odor (like bananas) to their formalin? > Is it a strange solvent? The idea of it being amyl acetate has been bounced > around, but I cannot see a histologic use for such a chemical. > > Thanks for your input. > > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology & Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > plaurie@cellnetix.com > From anonwums1 <@t> gmail.com Mon Jan 24 17:48:46 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Jan 24 17:48:51 2011 Subject: [Histonet] Re: Banana smelling chemical in our formalin In-Reply-To: References: Message-ID: That wasn't formalin. It was Histologie for Men, the new banana-scented cologne for histotechs. But seriously, I guess it's formalin ethyl acetate, which smells vaguely banana-like and is used for extraction of parasites from stool samples. Adam On Mon, Jan 24, 2011 at 4:56 PM, Patrick Laurie wrote: > > Histonet, > > > > We get a number of our specimens from outreach clients. We received back > > from one of our sites a jug of "Formalin" to be recycled. However when > it > > was opened up it had a strong odor of bananas. I know we shouldn't smell > > the chemicals, but luckily this was caught before it was put through our > > recycler. I was wondering if anyone might have an idea what this could > be? > > Is there a manufacturer that adds an odor (like bananas) to their > formalin? > > Is it a strange solvent? The idea of it being amyl acetate has been > bounced > > around, but I cannot see a histologic use for such a chemical. > > > > Thanks for your input. > > > > -- > > Patrick Laurie HT(ASCP)QIHC > > CellNetix Pathology & Laboratories > > 1124 Columbia Street, Suite 200 > > Seattle, WA 98104 > > plaurie@cellnetix.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald <@t> mtsac.edu Mon Jan 24 16:15:11 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jan 24 18:09:25 2011 Subject: [Histonet] gram stain Message-ID: Does anyone have a method for the Twort stain or a gram stain that does not use picric acid or ether? We were using a Twort stain and I cannot find the original reference for it and we suspect we have one of the concentrations wrong. Thank you, Jennifer MacDonald From jnocito <@t> satx.rr.com Mon Jan 24 18:13:54 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jan 24 18:14:16 2011 Subject: [Histonet] Archives Message-ID: Greetings all, I'm going to show my ignorance (like that hasn't happened before). When accessing the archives, how do I do it? Do I search by topic or date? All the time I've been on here, I never had to use the archives. Thanks. Oh Oh, I feel another tear coming Joe From AnthonyH <@t> chw.edu.au Mon Jan 24 18:50:59 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jan 24 18:51:10 2011 Subject: [Histonet] gram stain In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715705FC39@xmdb02.nch.kids> Jennifer, Here is the method from our manual: Gram Stain Principle: Crystal violet stains up both Gram positive and Gram negative bacterial wall but only the Gram positive wall structure is capable of retaining the violet molecules that are locked in by iodine. Gram negative cell wall loses the violet colour when differentiated with acetone, and stains up red as a result of counterstaining. Fixation: 10% buffered formalin. Microtomy: Paraffin sections at 5?m. Controls: Placenta containing gram positive cocci and gram negative bacilli Reagents: 1. 1% Crystal Violet - Warning: Flammable, Suspected Carcinogen - see MSDS Crystal Violet (Or Methyl Violet) 10g Ethanol 100 ml 1% Ammonium Oxalate 400 ml 2. Lugol's Iodine - Warning: Toxic - see MSDS 3. Acetone - Warning: Flammable - see MSDS 4. Twort's counterstain: 0.2% neutral red (CI 50040) in ethanol 9ml 0.2% fast green (CI 42053) in ethanol 1ml Distilled water 30ml Mix immediately before use. Procedure: 1. Bring sections to distilled water. 2. Cover sections with crystal violet 30-60 seconds 3. Rinse slides in water 4. Stain with Lugol's iodine 30-60 seconds 5. Tap water wash thoroughly 6. Blot sections lightly to remove excess water 7. Differentiate with acetone until dye stops running off the section. 8. Wash thoroughly with water 9. Counterstain Tworts 10 minutes 10. Wash, dehydrate quickly, clear and mount. Results: Gram positive bacteria violet blue Gram negative bacteria red Surrounding connective tissue green Reference: 1. Preston, Morrell. J. Path. Bact. (1962), 84:241-5. 2. Twort, J. State. Medicine (1924), 32:351. 3. Cherukian, Schenk. J. Histotech. (1982), 5(3):127-128. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, 25 January 2011 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gram stain Does anyone have a method for the Twort stain or a gram stain that does not use picric acid or ether? We were using a Twort stain and I cannot find the original reference for it and we suspect we have one of the concentrations wrong. Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From bakevictoria <@t> gmail.com Mon Jan 24 18:59:22 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Jan 24 18:59:28 2011 Subject: [Histonet] Archives In-Reply-To: References: Message-ID: Hi - I go by subject matter, but there was a time (a very long time ago) where they had them by date as well. When in doubt I just wing it and somehow I find what I'm looking for. Vikki On Jan 24, 2011 7:14 PM, "Joe Nocito" wrote: > Greetings all, > I'm going to show my ignorance (like that hasn't happened before). When accessing the archives, how do I do it? Do I search by topic or date? All the time I've been on here, I never had to use the archives. Thanks. Oh Oh, I feel another tear coming > > Joe > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micro <@t> superlink.net Mon Jan 24 20:45:42 2011 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Mon Jan 24 20:46:00 2011 Subject: [Histonet] Re: Banana smelling chemical in our formalin References: Message-ID: It's Amyl Acetate. ----- Original Message ----- From: "Adam ." To: "Patrick Laurie" Cc: Sent: Monday, January 24, 2011 6:48 PM Subject: Re: [Histonet] Re: Banana smelling chemical in our formalin > That wasn't formalin. It was Histologie for Men, the new banana-scented > cologne for histotechs. > > But seriously, I guess it's formalin ethyl acetate, which smells vaguely > banana-like and is used for extraction of parasites from stool samples. > > Adam > > On Mon, Jan 24, 2011 at 4:56 PM, Patrick Laurie > wrote: > >> > Histonet, >> > >> > We get a number of our specimens from outreach clients. We received >> > back >> > from one of our sites a jug of "Formalin" to be recycled. However when >> it >> > was opened up it had a strong odor of bananas. I know we shouldn't >> > smell >> > the chemicals, but luckily this was caught before it was put through >> > our >> > recycler. I was wondering if anyone might have an idea what this could >> be? >> > Is there a manufacturer that adds an odor (like bananas) to their >> formalin? >> > Is it a strange solvent? The idea of it being amyl acetate has been >> bounced >> > around, but I cannot see a histologic use for such a chemical. >> > >> > Thanks for your input. >> > >> > -- >> > Patrick Laurie HT(ASCP)QIHC >> > CellNetix Pathology & Laboratories >> > 1124 Columbia Street, Suite 200 >> > Seattle, WA 98104 >> > plaurie@cellnetix.com >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald <@t> mtsac.edu Mon Jan 24 22:04:07 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jan 24 22:04:15 2011 Subject: [Histonet] gram stain In-Reply-To: <6D6BD1DE8A5571489398B392A38A715705FC39@xmdb02.nch.kids> Message-ID: Thank you!! Tony Henwood Sent by: histonet-bounces@lists.utsouthwestern.edu 01/24/2011 04:54 PM To "'Jennifer MacDonald'" , "histonet@lists.utsouthwestern.edu" cc Subject RE: [Histonet] gram stain Jennifer, Here is the method from our manual: Gram Stain Principle: Crystal violet stains up both Gram positive and Gram negative bacterial wall but only the Gram positive wall structure is capable of retaining the violet molecules that are locked in by iodine. Gram negative cell wall loses the violet colour when differentiated with acetone, and stains up red as a result of counterstaining. Fixation: 10% buffered formalin. Microtomy: Paraffin sections at 5?m. Controls: Placenta containing gram positive cocci and gram negative bacilli Reagents: 1. 1% Crystal Violet - Warning: Flammable, Suspected Carcinogen - see MSDS Crystal Violet (Or Methyl Violet) 10g Ethanol 100 ml 1% Ammonium Oxalate 400 ml 2. Lugol's Iodine - Warning: Toxic - see MSDS 3. Acetone - Warning: Flammable - see MSDS 4. Twort's counterstain: 0.2% neutral red (CI 50040) in ethanol 9ml 0.2% fast green (CI 42053) in ethanol 1ml Distilled water 30ml Mix immediately before use. Procedure: 1. Bring sections to distilled water. 2. Cover sections with crystal violet 30-60 seconds 3. Rinse slides in water 4. Stain with Lugol's iodine 30-60 seconds 5. Tap water wash thoroughly 6. Blot sections lightly to remove excess water 7. Differentiate with acetone until dye stops running off the section. 8. Wash thoroughly with water 9. Counterstain Tworts 10 minutes 10. Wash, dehydrate quickly, clear and mount. Results: Gram positive bacteria violet blue Gram negative bacteria red Surrounding connective tissue green Reference: 1. Preston, Morrell. J. Path. Bact. (1962), 84:241-5. 2. Twort, J. State. Medicine (1924), 32:351. 3. Cherukian, Schenk. J. Histotech. (1982), 5(3):127-128. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, 25 January 2011 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gram stain Does anyone have a method for the Twort stain or a gram stain that does not use picric acid or ether? We were using a Twort stain and I cannot find the original reference for it and we suspect we have one of the concentrations wrong. Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Tue Jan 25 00:46:47 2011 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Jan 25 00:47:35 2011 Subject: [Histonet] in situ - thanks & 1 more query Message-ID: Hi all, just wanted to say thanks for all the info received. L Looking through the protocols it appears that i need an anti-DIG antibody, preferably HRP labelled - any suggestions as to supplier? I remember using one from DAKO, but don't see it in the latest catalogue. Please - any sugestions as to where I can get it? Thanks again - you guys (and gals) rock!! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Michel.Bataillon <@t> citox.com Tue Jan 25 04:16:06 2011 From: Michel.Bataillon <@t> citox.com (Bataillon Michel) Date: Tue Jan 25 04:16:50 2011 Subject: [Histonet] g ratio Message-ID: <1FB9362B907EEC43959F72445AC63B637CFC4C@apollon.cit_serveur.com> Hello, We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware. Do you think that we can perform this work with thin paraffin slides - 1.5?m (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks. Thank you, Michel Michel BATAILLON Histology Evreux, France ************************************************************* Ce message et toutes les pi?ces jointes sont confidentiels et ?tablis ? l'attention exclusive de ses destinataires. Si vous recevez ce message par erreur, merci de le d?truire et d'en avertir imm?diatement l'exp?diteur. Toute utilisation de ce message non conforme ? sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. Internet ne permettant pas de garantir l'int?grit? de ce message, le CIT d?cline toute responsabilit? au titre de ce message s'il a ?t? alt?r?, d?form? ou falsifi?. Prot?geons ensemble l'environnement, n'imprimons ce mail (et ses pi?ces jointes) que si cela est n?cessaire. This transmission and any attachments are confidential and intended solely for the use of the addressee(s). If you are not an intended recipient, please notify us immediately by replying to the message and deleting it from your computer. In this case, any unauthorized use, copying or distribution is strictly prohibited. Please be aware that E-mails are susceptible to alteration and their integrity cannot be guaranteed. CIT shall not be liable for this E-mail if corrupted, changed or falsified. Please consider the environment before printing this E-mail. ************************************************************* From cgill <@t> marylandgeneral.org Tue Jan 25 06:51:11 2011 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Tue Jan 25 06:51:14 2011 Subject: [Histonet] Animal tissue In-Reply-To: References: Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9F24@MDGEN-EXCH1.marylandgeneral.org> Thanks for that info. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Thursday, January 20, 2011 9:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal tissue If you are processing mammalian tissue, it can be run with your regular "human' tissues. Rodent tissues are totally different and require shorter processing times. As long as your animal tissues are grossed at the proper thickness you should have no trouble. Cheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Tue Jan 25 08:19:23 2011 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Tue Jan 25 08:19:28 2011 Subject: [Histonet] Re: Banana smelling chemical in our formalin In-Reply-To: Message-ID: <904917357.996374.1295965163828.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I agree with Markus, someone may used amyl acetate and did not tell you.? If you have a reagent supplier who got hold of a bad batch of SDA reagent alcohol that was not a true combination of ethanol, methanol and isopropanol but a chemically denatured (for electronics or other manufacturing) grade it may have ethyl acetate in it.? I would start checking with my suppliers first as the cheaper grades of SDA are not suitable for Histology use.? They are attractive price wise if you don't know what you are buying or understand what is needed in Histology. Pam Marcum UAMS ----- Original Message ----- From: "Markus F. Meyenhofer" To: "Adam ." , "Patrick Laurie" Cc: histonet@lists.utsouthwestern.edu Sent: Monday, January 24, 2011 8:45:42 PM Subject: Re: [Histonet] Re: Banana smelling chemical in our formalin It's Amyl Acetate. ----- Original Message ----- From: "Adam ." To: "Patrick Laurie" Cc: Sent: Monday, January 24, 2011 6:48 PM Subject: Re: [Histonet] Re: Banana smelling chemical in our formalin > That wasn't formalin. It was Histologie for Men, the new banana-scented > cologne for histotechs. > > But seriously, I guess it's formalin ethyl acetate, which smells vaguely > banana-like and is used for extraction of parasites from stool samples. > > Adam > > On Mon, Jan 24, 2011 at 4:56 PM, Patrick Laurie > wrote: > >> > Histonet, >> > >> > We get a number of our specimens from outreach clients. ?We received >> > back >> > from one of our sites a jug of "Formalin" to be recycled. ?However when >> it >> > was opened up it had a strong odor of bananas. ?I know we shouldn't >> > smell >> > the chemicals, but luckily this was caught before it was put through >> > our >> > recycler. ?I was wondering if anyone might have an idea what this could >> be? >> > Is there a manufacturer that adds an odor (like bananas) to their >> formalin? >> > Is it a strange solvent? ?The idea of it being amyl acetate has been >> bounced >> > around, but I cannot see a histologic use for such a chemical. >> > >> > Thanks for your input. >> > >> > -- >> > Patrick Laurie HT(ASCP)QIHC >> > CellNetix Pathology & Laboratories >> > 1124 Columbia Street, Suite 200 >> > Seattle, WA 98104 >> > plaurie@cellnetix.com >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Markus F. Meyenhofer" To: "Adam ." , "Patrick Laurie" Cc: histonet@lists.utsouthwestern.edu Sent: Monday, January 24, 2011 8:45:42 PM Subject: Re: [Histonet] Re: Banana smelling chemical in our formalin It's Amyl Acetate. ----- Original Message ----- From: "Adam ." To: "Patrick Laurie" Cc: Sent: Monday, January 24, 2011 6:48 PM Subject: Re: [Histonet] Re: Banana smelling chemical in our formalin > That wasn't formalin. It was Histologie for Men, the new banana-scented > cologne for histotechs. > > But seriously, I guess it's formalin ethyl acetate, which smells vaguely > banana-like and is used for extraction of parasites from stool samples. > > Adam > > On Mon, Jan 24, 2011 at 4:56 PM, Patrick Laurie > wrote: > >> > Histonet, >> > >> > We get a number of our specimens from outreach clients. ?We received >> > back >> > from one of our sites a jug of "Formalin" to be recycled. ?However when >> it >> > was opened up it had a strong odor of bananas. ?I know we shouldn't >> > smell >> > the chemicals, but luckily this was caught before it was put through >> > our >> > recycler. ?I was wondering if anyone might have an idea what this could >> be? >> > Is there a manufacturer that adds an odor (like bananas) to their >> formalin? >> > Is it a strange solvent? ?The idea of it being amyl acetate has been >> bounced >> > around, but I cannot see a histologic use for such a chemical. >> > >> > Thanks for your input. >> > >> > -- >> > Patrick Laurie HT(ASCP)QIHC >> > CellNetix Pathology & Laboratories >> > 1124 Columbia Street, Suite 200 >> > Seattle, WA 98104 >> > plaurie@cellnetix.com >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Jan 25 08:33:05 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jan 25 08:30:03 2011 Subject: [Histonet] g ratio In-Reply-To: <1FB9362B907EEC43959F72445AC63B637CFC4C@apollon.cit_serveur.com> References: <1FB9362B907EEC43959F72445AC63B637CFC4C@apollon.cit_serveur.com> Message-ID: <4D3EDF21.60103@umdnj.edu> If you want to get the results of your study published in a reputable journal you should use the best available method, ie. epoxy sections. Geoff On 1/25/2011 5:16 AM, Bataillon Michel wrote: > Hello, > We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware. > Do you think that we can perform this work with thin paraffin slides - 1.5?m (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks. > Thank you, > Michel > > Michel BATAILLON > Histology > Evreux, France > > ************************************************************* > > Ce message et toutes les pi?ces jointes sont confidentiels et ?tablis ? l'attention exclusive de ses destinataires. > Si vous recevez ce message par erreur, merci de le d?truire et d'en avertir imm?diatement l'exp?diteur. > Toute utilisation de ce message non conforme ? sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. > Internet ne permettant pas de garantir l'int?grit? de ce message, le CIT d?cline toute responsabilit? au titre de ce message s'il a ?t? alt?r?, d?form? ou falsifi?. > > Prot?geons ensemble l'environnement, n'imprimons ce mail (et ses pi?ces jointes) que si cela est n?cessaire. > > > This transmission and any attachments are confidential and intended solely for the use of the addressee(s). > If you are not an intended recipient, please notify us immediately by replying to the message and deleting it from your computer. > In this case, any unauthorized use, copying or distribution is strictly prohibited. > Please be aware that E-mails are susceptible to alteration and their integrity cannot be guaranteed. > CIT shall not be liable for this E-mail if corrupted, changed or falsified. > > Please consider the environment before printing this E-mail. > > ************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mhale <@t> carisls.com Tue Jan 25 08:37:10 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Jan 25 08:37:14 2011 Subject: [Histonet] OHIO HT Position Message-ID: <6F33D8418806044682A391273399860F06B3083D@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamargastro.com Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From talulahgosh <@t> gmail.com Tue Jan 25 08:37:46 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jan 25 08:37:49 2011 Subject: [Histonet] in situ - thanks & 1 more query In-Reply-To: References: Message-ID: The best place to look for antibodies from different companies is biocompare.com. They have a separate antibody search engine. That said, I'm sure Roche Diagnostics or Invitrogen has one. Roche is the original maker of the DIG system (at least, I think they are). Also, Jackson Immunoresearch has really good prices on antibodies and they always work. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Tue, Jan 25, 2011 at 1:46 AM, louise renton wrote: > Hi all, just wanted to say thanks for all the info received. L > Looking through the protocols it appears that i need an anti-DIG antibody, > preferably HRP labelled - any suggestions as to supplier? I remember using > one from DAKO, but don't see it in the latest catalogue. Please - any > sugestions as to where I can get it? > > Thanks again - you guys (and gals) rock!! > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Tue Jan 25 08:44:42 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jan 25 08:44:45 2011 Subject: [Histonet] Archives In-Reply-To: References: Message-ID: Honestly, I would just use google and search with histonet and whatever your looking for. Much easier! It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Mon, Jan 24, 2011 at 7:59 PM, Victoria Baker wrote: > Hi - I go by subject matter, but there was a time (a very long time ago) > where they had them by date as well. When in doubt I just wing it and > somehow I find what I'm looking for. > > Vikki > > On Jan 24, 2011 7:14 PM, "Joe Nocito" wrote: > > Greetings all, > > I'm going to show my ignorance (like that hasn't happened before). When > accessing the archives, how do I do it? Do I search by topic or date? All > the time I've been on here, I never had to use the archives. Thanks. Oh Oh, > I feel another tear coming > > > > Joe > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From naje1972 <@t> yahoo.com Tue Jan 25 09:53:00 2011 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Tue Jan 25 09:53:05 2011 Subject: [Histonet] part time histology job in chicago Message-ID: <761538.66161.qm@web110615.mail.gq1.yahoo.com> We have a part time histology position located at Gottlieb memorial hospital in Melrose Park, Illlinois; if anyone is interested in a part time histology please give us a call at 708-618-3200 ext 2012. You can also go to the Gottlieb website and fill out the application and submit your resume there as well; if you go that route your application and resume will go directly to the lab manager and she will forward it to me. Our fax number is 708-681-7874. Thank you Cynthia Haynes-James H.T. From asmith <@t> mail.barry.edu Tue Jan 25 10:34:52 2011 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Jan 25 10:34:59 2011 Subject: [Histonet] re: storing acetic acid Message-ID: We have separate storage cupboards for inorganic acids (many of which are oxidizing agents) and organic acids (many of which are flammable). If we didn't have an organic acids cupboard, we would store it with organic solvents. The reaction of acetic acid with nitric acid might be violent, the reaction of acetic acid with ethanol is slow and quiet. > -Allen A. Smith > Professor of Anatomy > Barry University School of Podiatric Medicine From rsrichmond <@t> gmail.com Tue Jan 25 10:31:49 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Jan 25 10:42:46 2011 Subject: [Histonet] Re: Banana smelling chemical in our formalin Message-ID: Amyl acetate ("banana oil") is produced by an alternate fermentation pathway by some of the yeasts used to make Hefeweizen type beers. The thought of recycling such a fine beer horrifies me. Ethyl acetate, formed by esterification of ethanol with acetic acid as fixatives such as Davidson's and O-Fix age, smells like airplane dope (if you're old enough to remember airplane dope) or nail polish remover (if you aren't). Obviously these fixatives could easily be mixed with formalin for recycling, since they also contain formaldehyde. About 25 years ago there was a fad for "odor masked" formaldehyde solutions. The ones I used smelled even worse than formaldehyde, but they didn't smell like banana oil. I don't know if these solutions are still available. Bob Richmond Samurai Pathologist Knoxville TN From rsrichmond <@t> gmail.com Tue Jan 25 10:49:57 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Jan 25 10:55:31 2011 Subject: [Histonet] Re: gram stain Message-ID: The original Brown-Hopps tissue gram stain was published in Lee Luna's AFIP Manual, 3rd ed. 1968. It did not require picric acid, ethyl ether, or acetone. I remember that the stain was widely used after that. The AFIP manual method does require ethylene glycol monoethyl ether (Cellosolve 1), which is less of a fire hazard than is ethyl ether. See the MSDS at http://www.jtbaker.com/msds/englishhtml/e2600.htm before ordering it, though. Freida Carson (in the 2nd edition of her book - I don't have the 3rd) restored the picric acid and acetone in her modification of the Brown-Hopps method. Putting on my pathologist hat now - tissue gram stains are greatly overrated - they don't work nearly as well as they do on smears. If you want to see or count bacteria in tissue sections, use a simple blue stain (toluidine blue, or Diff-Quik II or its generic equivalent). Bob Richmond Samurai Pathologist Knoxville TN From Ronald.Houston <@t> nationwidechildrens.org Tue Jan 25 11:13:16 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Jan 25 11:14:27 2011 Subject: [Histonet] RE: g ratio In-Reply-To: <1FB9362B907EEC43959F72445AC63B637CFC4C@apollon.cit_serveur.com> References: <1FB9362B907EEC43959F72445AC63B637CFC4C@apollon.cit_serveur.com> Message-ID: Michel, Most studies involving morphometric studies of peripheral nerves involve "thick" plastic sections stained with toluidine blue. In my opinion, you are unlikely to get consistent and acceptable staining results on paraffin sections for the simple reason that the intense blue staining on thick EM sections is more from the osmium tetroxide than the toluidine blue per se. Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bataillon Michel Sent: Tuesday, January 25, 2011 5:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] g ratio Hello, We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware. Do you think that we can perform this work with thin paraffin slides - 1.5?m (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks. Thank you, Michel Michel BATAILLON Histology Evreux, France ************************************************************* Ce message et toutes les pi?ces jointes sont confidentiels et ?tablis ? l'attention exclusive de ses destinataires. Si vous recevez ce message par erreur, merci de le d?truire et d'en avertir imm?diatement l'exp?diteur. Toute utilisation de ce message non conforme ? sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. Internet ne permettant pas de garantir l'int?grit? de ce message, le CIT d?cline toute responsabilit? au titre de ce message s'il a ?t? alt?r?, d?form? ou falsifi?. Prot?geons ensemble l'environnement, n'imprimons ce mail (et ses pi?ces jointes) que si cela est n?cessaire. This transmission and any attachments are confidential and intended solely for the use of the addressee(s). If you are not an intended recipient, please notify us immediately by replying to the message and deleting it from your computer. In this case, any unauthorized use, copying or distribution is strictly prohibited. Please be aware that E-mails are susceptible to alteration and their integrity cannot be guaranteed. CIT shall not be liable for this E-mail if corrupted, changed or falsified. Please consider the environment before printing this E-mail. ************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From jkiernan <@t> uwo.ca Tue Jan 25 14:04:26 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Jan 25 14:04:29 2011 Subject: [Histonet] Re: Storing acetic acid *** Message-ID: The rule about storing "corrosives" and "alcohols" together is to prevent the accidental mixing of concentrated nitric acid with ethanol. This could occur if an earthquake shakes and breaks the bottles in a confined space. Schoolboys in the 1950s knew all about this and other interesting explosive chemical reactions, back in the days when elementary chemistry was more than just arithmetic. We had fun! Acetic acid can burn. I was surprised to read that its flash point (39C) is similar to that of gasolene [petrol] (40-70C). The flash point is the lowest temperature at which a spark (?>800C) just above the surface will set the liquid on fire. For comparison, the flash point of xylene is 29C, ethanol 18C and acetone (a serious fire hazard) minus18C. Data from the Merck Index, 12th ed, 1996. Acetic acid freezes at 17C (hence the name "glacial") but it contracts as this happens, so it doesn't break the bottle as can happen with freezing water. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Robert Richmond Date: Saturday, January 22, 2011 22:26 Subject: [Histonet] Re: Storing acetic acid To: histonet@lists.utsouthwestern.edu > Laurie Colbert (where?) asks: > > > I am trying to find out how to store acetic acid. We were > storing it in > > our acid cabinet with the other acids, but we were inspected > by the fire > > department last week and they said that acetic acid is > flammable and a > > corrosive and that it should be stored with the flammables. > But my > > manager is saying that you can't store corrosives and alcohols > together,> so I'm not sure where it should actually be stored > (other than in some > > cabinet all by itself)! > > The Fisher Scientific MSDS - at > http://fscimage.fishersci.com/msds/00120.htm > advises: > > Storage: Keep away from heat, sparks, and flame. Keep from > contactwith oxidizing materials. Store in a cool, dry, well- > ventilated area > away from incompatible substances. Do not store near alkaline > substances. Acetic acid should be kept above its freezing point > of 62 > degrees F (17 degrees C) to allow it to be handled as a liquid. It > will contract slightly on freezing. Freezing and thawing does not > affect product quality. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Tue Jan 25 14:05:55 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Jan 25 14:06:00 2011 Subject: [Histonet] MOHS Tech/Histotech Needed in OH Message-ID: *Position:* Histotechnician, Histotechnologist, Histology Technician, MOHS Technician *Location: *Westerville, OH-Outside of Columbus, OH large 10 physician practice *Schedule:* Full Time, Direct (Permanent) Hire. Employee will be scheduled between 3-4 days when the MOHS surgeon is scheduled for MOHS. On the rare occasion he/she will be scheduled 2 days for MOHS surgery. On the days the MOHS surgeon is not doing MOHS, that time will be spent in the Dermpath Laboratory. Day Shift. *Environment: * *A* large comprehensive dermatology practice, established in 1980 who takes pride in providing high quality care and professional service. *Requirements:* - Ability to assist with CLIA documentation - Experience with both frozen sections & paraffin - Candidates looking for long term employment - At least 2-5 years experience working with frozen sections for MOHS surgery & Paraffin. *Benefits:* * * Medical coverage that is paid 90% by the company, 10% by the employee. Dental and vision plans that can be obtained in addition Disability coverage that can be obtained in addition Company paid life insurance policy Paid Holidays Vacation Time 401K program that are always contributed to by doctors Sick time Personal Day(s) Uniform Reimbursement *To Apply:* * * To apply for this position please send resume to alyssa@alliedsearchpartners.com , salary requirements, and availability to speak to one of our recruiters directly. Thank you!!! -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From jkiernan <@t> uwo.ca Tue Jan 25 14:21:20 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Jan 25 14:21:23 2011 Subject: [Histonet] RE: g ratio Message-ID: A long time ago a graduate student in my lab, Bruce Stelmack, did this with 4um transverse paraffin sections of glutaraldehyde-fixed and postosmicated rat facial nerves. The myelin sheaths were significantly thicker in rats treated with a thyroid hormone than in controls. This was written up in 1977: "Effects of triiodothyronine on normal and regenerating facial nerve of the rat." Acta neuropathologica 40: 151-155. This paper doesn't have photos but it does give tables of measurements. It is available on the SpringerLink web site: http://www.springerlink.com/content/0001/6322/40/2/ Although it was possible to measure myelinated nerve fibres in paraffin sections, resin embedding would have been preferable, if only because the sections can be much thinner and the boundaries are sharper. However you embed, all the measurements will be smaller than the sizes in vivo, and there is no certain way to know the amount of shrinkage. The measurements are of value only for comparing sections of identically processed specimens. John Kiernan Anatomy, UWO London, Canada = = = Hello, We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware. Do you think that we can perform this work with thin paraffin slides - 1.5?m (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks. Thank you, Michel Michel BATAILLON Histology Evreux, France ________________________- Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MHuth <@t> baymedical.org Tue Jan 25 14:22:03 2011 From: MHuth <@t> baymedical.org (Huth, Myra) Date: Tue Jan 25 14:22:11 2011 Subject: [Histonet] cutting replacement patches In-Reply-To: Message-ID: <4565810021302841A3599A8F7CCE820F0A60583A@BMCEXCHVS1.corp.baymedical.org> Hi All, Does anyone know a company that sells cutting patches? The ones we use are black with an adhesive back that "sticks" to our cutting board. Seems like the company we were getting them from no longer carries them.. Thanks for your help Myra Huth,HT(ASCP) Bay Medical Center Pathology Dept. Panama City, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, January 25, 2011 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 86, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. re: storing acetic acid (Smith, Allen) 2. Re: Banana smelling chemical in our formalin (Robert Richmond) 3. Re: gram stain (Robert Richmond) 4. RE: g ratio (Houston, Ronald) ---------------------------------------------------------------------- Message: 1 Date: Tue, 25 Jan 2011 16:34:52 +0000 From: "Smith, Allen" Subject: [Histonet] re: storing acetic acid To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have separate storage cupboards for inorganic acids (many of which are oxidizing agents) and organic acids (many of which are flammable). If we didn't have an organic acids cupboard, we would store it with organic solvents. The reaction of acetic acid with nitric acid might be violent, the reaction of acetic acid with ethanol is slow and quiet. > -Allen A. Smith > Professor of Anatomy > Barry University School of Podiatric Medicine ------------------------------ Message: 2 Date: Tue, 25 Jan 2011 11:31:49 -0500 From: Robert Richmond Subject: [Histonet] Re: Banana smelling chemical in our formalin To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Amyl acetate ("banana oil") is produced by an alternate fermentation pathway by some of the yeasts used to make Hefeweizen type beers. The thought of recycling such a fine beer horrifies me. Ethyl acetate, formed by esterification of ethanol with acetic acid as fixatives such as Davidson's and O-Fix age, smells like airplane dope (if you're old enough to remember airplane dope) or nail polish remover (if you aren't). Obviously these fixatives could easily be mixed with formalin for recycling, since they also contain formaldehyde. About 25 years ago there was a fad for "odor masked" formaldehyde solutions. The ones I used smelled even worse than formaldehyde, but they didn't smell like banana oil. I don't know if these solutions are still available. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Tue, 25 Jan 2011 11:49:57 -0500 From: Robert Richmond Subject: [Histonet] Re: gram stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The original Brown-Hopps tissue gram stain was published in Lee Luna's AFIP Manual, 3rd ed. 1968. It did not require picric acid, ethyl ether, or acetone. I remember that the stain was widely used after that. The AFIP manual method does require ethylene glycol monoethyl ether (Cellosolve 1), which is less of a fire hazard than is ethyl ether. See the MSDS at http://www.jtbaker.com/msds/englishhtml/e2600.htm before ordering it, though. Freida Carson (in the 2nd edition of her book - I don't have the 3rd) restored the picric acid and acetone in her modification of the Brown-Hopps method. Putting on my pathologist hat now - tissue gram stains are greatly overrated - they don't work nearly as well as they do on smears. If you want to see or count bacteria in tissue sections, use a simple blue stain (toluidine blue, or Diff-Quik II or its generic equivalent). Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Tue, 25 Jan 2011 12:13:16 -0500 From: "Houston, Ronald" Subject: [Histonet] RE: g ratio To: 'Bataillon Michel' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Michel, Most studies involving morphometric studies of peripheral nerves involve "thick" plastic sections stained with toluidine blue. In my opinion, you are unlikely to get consistent and acceptable staining results on paraffin sections for the simple reason that the intense blue staining on thick EM sections is more from the osmium tetroxide than the toluidine blue per se. Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bataillon Michel Sent: Tuesday, January 25, 2011 5:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] g ratio Hello, We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware. Do you think that we can perform this work with thin paraffin slides - 1.5?m (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks. Thank you, Michel Michel BATAILLON Histology Evreux, France ************************************************************* Ce message et toutes les pi?ces jointes sont confidentiels et ?tablis ? l'attention exclusive de ses destinataires. Si vous recevez ce message par erreur, merci de le d?truire et d'en avertir imm?diatement l'exp?diteur. Toute utilisation de ce message non conforme ? sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. Internet ne permettant pas de garantir l'int?grit? de ce message, le CIT d?cline toute responsabilit? au titre de ce message s'il a ?t? alt?r?, d?form? ou falsifi?. Prot?geons ensemble l'environnement, n'imprimons ce mail (et ses pi?ces jointes) que si cela est n?cessaire. 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If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 86, Issue 33 **************************************** -------------------------------------------------------- Confidentiality Notice: The information contained in this e-mail is intended solely for the person(s) to whom it is addressed. This information is the property of Bay Medical Center and may be confidential. If you are not the intended addressee, you should not distribute, copy or disclose this e-mail. Please notify the sender immediately if you received this e-mail by mistake and delete this email from your system and destroy all printed copies. From sprice2003 <@t> gmail.com Tue Jan 25 14:24:16 2011 From: sprice2003 <@t> gmail.com (Sally Price) Date: Tue Jan 25 14:24:22 2011 Subject: [Histonet] in situ - thanks & 1 more query Message-ID: Our anti-Dig reagent is included in Biocare's ISH detction kit. Its unlabled, but is detected with a secondary-polymer-HRP reagent that's directed at the anti-Dig primary. ------------------------------ Message: 14 Date: Tue, 25 Jan 2011 08:46:47 +0200 From: louise renton Subject: [Histonet] in situ - thanks & 1 more query To: Histonet Histonet@lists.utsouthwestern.edu Hi all, just wanted to say thanks for all the info received. L Looking through the protocols it appears that i need an anti-DIG antibody, preferably HRP labelled - any suggestions as to supplier? I remember using one from DAKO, but don't see it in the latest catalogue. Please - any sugestions as to where I can get it? Thanks again - you guys (and gals) rock!! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From CIngles <@t> uwhealth.org Tue Jan 25 18:47:45 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Jan 25 18:49:34 2011 Subject: [Histonet] Re: Storing acetic acid *** References: Message-ID: GEE, and all us young, wet behind the ears techs thank goodness you are here to keep us on the straight and narrow.(OK, maybe not the second part...) I learn so much from you guys. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of John Kiernan Sent: Tue 1/25/2011 2:04 PM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Storing acetic acid *** Schoolboys in the 1950s knew all about this and other interesting explosive chemical reactions, back in the days when elementary chemistry was more than just arithmetic. We had fun! From meljdelbarrio <@t> yahoo.com Tue Jan 25 22:29:32 2011 From: meljdelbarrio <@t> yahoo.com (Mel John del Barrio) Date: Tue Jan 25 22:29:36 2011 Subject: [Histonet] GLX Linistain for Alcian Blue-PAS, PAS, AB's Message-ID: <150929.3616.qm@web114514.mail.gq1.yahoo.com> Hi Anyone here using GLX?Linistain for Special Stains like AB's ABPAS and PAS?? Thanks, MJ del Barrio? Image by FlamingText.com From sfonner <@t> labpath.com Wed Jan 26 08:45:42 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Wed Jan 26 08:48:19 2011 Subject: [Histonet] FX111a Protocol Message-ID: <001301cbbd67$b52a4ac0$1f7ee040$@com> Does anyone have a current protocol for Factor X111a on the Ventana Ultra that is working for you? I would appreciate any info or support you could give. We are using the AC-1A1 clone from Cell Marque for derms. Thanks! From 41dmb41 <@t> gmail.com Wed Jan 26 09:22:41 2011 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Wed Jan 26 10:08:59 2011 Subject: [Histonet] Sunquest CoPath Rep Message-ID: Could any Sunquest CoPath sales rep please contact me privately off the list? Thanks, Drew Meyer From GaleL <@t> unionhospital.org Wed Jan 26 10:30:01 2011 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Wed Jan 26 10:30:11 2011 Subject: [Histonet] used Leica XL Message-ID: I've been asked to get pricing for possible purchase of a good used/refurbished Leica autostainer XL. Gale Limron CT, HT (ASCP) Histology Supervisor This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From nicole <@t> dlcjax.com Wed Jan 26 10:46:01 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Jan 26 10:46:10 2011 Subject: [Histonet] Leica autostainer XL Message-ID: <4312.208.62.167.196.1296060361.squirrel@webmail.realpages.com> Does anyone know if you can just deparaffinize slides in the stainer. Im just wondering if I also need a small stain line to dewax specials. Thank you, Nicole From STACEY.LANGENBERG <@t> UCDENVER.EDU Wed Jan 26 10:52:43 2011 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Wed Jan 26 10:52:49 2011 Subject: [Histonet] Leica autostainer XL Message-ID: <1449F015-1E6C-4741-8E04-417DB0237C6D@ucdenver.edu> You can set up a just depar run. Sent from myTouch 4G ----- Reply message ----- From: "Nicole Tatum" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Leica autostainer XL Date: Wed, Jan 26, 2011 9:48 am Does anyone know if you can just deparaffinize slides in the stainer. Im just wondering if I also need a small stain line to dewax specials. Thank you, Nicole _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Wed Jan 26 10:56:51 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Wed Jan 26 10:56:55 2011 Subject: [Histonet] FX111a Protocol In-Reply-To: <001301cbbd67$b52a4ac0$1f7ee040$@com> References: <001301cbbd67$b52a4ac0$1f7ee040$@com> Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA768369@CMHEXCC01MBX.childrensmemorial.org> Hi, We run FactorX111a clone AC-1A1 on the Ultra, here is our protocol: 1. De-paraffinazation (selected) 2. Cell conditioning #1 20 minutes, then 36 minutes. 3. Antibody for 24 minutes 4. Ultra wash (selected) 5. Hematoxylin 4 minutes 6. Bluing 4 minutes. Hope this helps. Lisa V. Children's Memorial Hospital Chicago, IL 60616 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Wednesday, January 26, 2011 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FX111a Protocol Does anyone have a current protocol for Factor X111a on the Ventana Ultra that is working for you? I would appreciate any info or support you could give. We are using the AC-1A1 clone from Cell Marque for derms. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jan 26 11:15:52 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jan 26 11:15:58 2011 Subject: [Histonet] Oil Red O for FS on Muscle Message-ID: <81CDB427-526C-445F-B80C-98E60585E3E2@yahoo.com> Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am working with them with pH issues. The Oil Red O is very problematic. They are mixing before use and filtering with 42 Watman paper, but there is a lot of residual background on the slides. Also, they were not fixing the sections with 37% formaldehyde, even though the procedure calls for it. Could you share who you get the stain from also. Your assistance is greatly appreciated. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From liz <@t> premierlab.com Wed Jan 26 11:23:14 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 26 11:23:23 2011 Subject: [Histonet] Oil Red O for FS on Muscle In-Reply-To: <81CDB427-526C-445F-B80C-98E60585E3E2@yahoo.com> Message-ID: Allison We make up our Oil Red O from scratch same day we use it, let it stand 10 minutes, and then filter with a Millipore Stericup 0.22?m, GP Express Plus Membrane, 250ml Receiver Bottle, catalog number: SCGPU02RE under vacuum. That seems to help a bit with the background and we are working with a really clear solution. We have not run muscles but aortic root and liver on mouse and rat. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 26, 2011 10:16 AM To: histonet Subject: [Histonet] Oil Red O for FS on Muscle Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am working with them with pH issues. The Oil Red O is very problematic. They are mixing before use and filtering with 42 Watman paper, but there is a lot of residual background on the slides. Also, they were not fixing the sections with 37% formaldehyde, even though the procedure calls for it. Could you share who you get the stain from also. Your assistance is greatly appreciated. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Wed Jan 26 11:23:29 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Wed Jan 26 11:23:34 2011 Subject: [Histonet] Oil Red O for FS on Muscle In-Reply-To: <81CDB427-526C-445F-B80C-98E60585E3E2@yahoo.com> References: <81CDB427-526C-445F-B80C-98E60585E3E2@yahoo.com> Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA76836C@CMHEXCC01MBX.childrensmemorial.org> Hi, We use Oil Red O - but we don't do it on muscle. Our protocol is we filter the whole bottle before we use it and then we apply it to unfixed slides for about 30 minutes. Rinse in tap water, counterstain with Hematoxylin and coverslip with Aquamount. We use it for cytospins and occasionally on various frozen tissues. Our stain is from American Mastertech. Hope this helps, Lisa V. Children's Memorial Hospital Chicago, IL 60616 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 26, 2011 11:16 AM To: histonet Subject: [Histonet] Oil Red O for FS on Muscle Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am working with them with pH issues. The Oil Red O is very problematic. They are mixing before use and filtering with 42 Watman paper, but there is a lot of residual background on the slides. Also, they were not fixing the sections with 37% formaldehyde, even though the procedure calls for it. Could you share who you get the stain from also. Your assistance is greatly appreciated. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Wed Jan 26 11:48:21 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Jan 26 11:49:29 2011 Subject: [Histonet] Leica autostainer XL In-Reply-To: <1449F015-1E6C-4741-8E04-417DB0237C6D@ucdenver.edu> References: <1449F015-1E6C-4741-8E04-417DB0237C6D@ucdenver.edu> Message-ID: <02DE42FF-793B-4CD6-AA8E-D949A3339900@imagesbyhopper.com> I have a run set up to bake the slides & run them down to water ( for my specials), another just to start at hematoxylin down to xylene etc. The stainer allows for nearly any custom program you can think of. :o) If you have any questions about how to set the program up, please let me know & I will be happy to help you with it. Michelle On Jan 26, 2011, at 11:52 AM, "Langenberg, Stacey" wrote: > You can set up a just depar run. > > Sent from myTouch 4G > > ----- Reply message ----- > From: "Nicole Tatum" > To: "histonet@lists.utsouthwestern.edu" > Subject: [Histonet] Leica autostainer XL > Date: Wed, Jan 26, 2011 9:48 am > > > > Does anyone know if you can just deparaffinize slides in the stainer. > > Im just wondering if I also need a small stain line to dewax specials. > > Thank you, > Nicole > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> email.arizona.edu Wed Jan 26 12:06:19 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jan 26 12:06:33 2011 Subject: [Histonet] Oil Red O for FS on Muscle In-Reply-To: <81CDB427-526C-445F-B80C-98E60585E3E2@yahoo.com> References: <81CDB427-526C-445F-B80C-98E60585E3E2@yahoo.com> Message-ID: <578C16BE-0020-467E-A4B3-363CF0CBC3E4@email.arizona.edu> I've done many of these stains and I get my ORO stain from Poly Scientific. Actually I use the whole kit and I use Freida's protocol in the second edition. That includes fixing in 37% formaldehyde. I filter the ORO just before use and usually get nice clean slides. If there is a residue it isn't much and wipes off easily. I even re-use the ORO stain once in a while and it still comes out great. My slides are often used for quantifying the amount of lipids so I don't counterstain most of the time. Andi Grantham On Jan 26, 2011, at 10:15 AM, Akemi Allison wrote: > Happy hump day! > > Does anyone have a good procedure for Oil Red O for FS on Muscle. > The procedure the lab I am working with is having a great deal of > problems with their muscle biopsy panel. I am trouble-shooting some > of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am > working with them with pH issues. > > The Oil Red O is very problematic. They are mixing before use and > filtering with 42 Watman paper, but there is a lot of residual > background on the slides. Also, they were not fixing the sections > with 37% formaldehyde, even though the procedure calls for it. Could > you share who you get the stain from also. Your assistance is > greatly appreciated. > > Akemi > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tjay30 <@t> yahoo.com Wed Jan 26 12:43:36 2011 From: tjay30 <@t> yahoo.com (Timothy Jay) Date: Wed Jan 26 12:43:39 2011 Subject: [Histonet] Histotech Needed in Santa Rosa, CA. Message-ID: <362826.34871.qm@web34304.mail.mud.yahoo.com> Certified Histotech needed in Santa Rosa, CA. Part-time (25-30 hrs/wk) opportunity for an experienced and certified tech to work in a brand new GI path lab.?Competitive pay and wonderful working environment. Send resumes to tjay30@yahoo.com. ? Kind Regards, ? Timothy Jay Pillar Consulting, LLC From Nacaela.Johnson <@t> USONCOLOGY.COM Wed Jan 26 13:42:01 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Wed Jan 26 13:43:07 2011 Subject: [Histonet] glass cleaner Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D4DF@txhous1eb012.uson.usoncology.int> Hello.... I am looking for suggestions on the best chemical cleaner for glassware that is used for special stains. Any ideas? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From candice_camille <@t> yahoo.com Wed Jan 26 13:47:05 2011 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Wed Jan 26 13:47:09 2011 Subject: [Histonet] (no subject) Message-ID: <894015.28954.qm@web62202.mail.re1.yahoo.com> Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille From histonet.nospam <@t> vneubert.com Wed Jan 26 13:51:55 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Jan 26 13:52:03 2011 Subject: [Histonet] glass cleaner In-Reply-To: <6DBD71C31D7E444482E5D3DFBC202D260245D4DF@txhous1eb012.uson.usoncology.int> References: <6DBD71C31D7E444482E5D3DFBC202D260245D4DF@txhous1eb012.uson.usoncology.int> Message-ID: <4D407B5B.607@vneubert.com> ~ 90 mL 70% EtOH ~ 10 mL 25% HCl Removed everything I ever had to deal with. After that give your glassware a good wash in the dishwasher, then rinse with plenty of demineralized water. And wear protective gloves, but as we all do, I actually don't have to remind any of the list members, right? ;) > Hello.... > > I am looking for suggestions on the best chemical cleaner for glassware > that is used for special stains. Any ideas? > > Thanks, > > Nacaela Johnson > Histology Technician > KCCC Pathology > 12000 110th St., Ste. 400 > Overland Park, KS 66210 > Office: 913-234-0576 > Fax: 913-433-7639 > Email: Nacaela.Johnson@USOncology.com > > The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Jan 26 13:54:09 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jan 26 13:54:13 2011 Subject: [Histonet] (no subject) In-Reply-To: <894015.28954.qm@web62202.mail.re1.yahoo.com> References: <894015.28954.qm@web62202.mail.re1.yahoo.com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47605@nmdamailsvr.nmda.ad.nmsu.edu> Go for HISTOLOGY!! Cytology requires a lot of Sitting in One Place Staring into a Microscope (please! - no offense to cytotechs - really!). With histology, your daily routine is varied and invigorating. And now I'm probably on some Cytology Tech Blacklist... sigh... From histonet.nospam <@t> vneubert.com Wed Jan 26 13:54:27 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Jan 26 13:54:33 2011 Subject: [Histonet] (no subject) In-Reply-To: <894015.28954.qm@web62202.mail.re1.yahoo.com> References: <894015.28954.qm@web62202.mail.re1.yahoo.com> Message-ID: <4D407BF3.1050602@vneubert.com> Is there a chance to go and see a lab which does histology? > Hello > > I am really new to the field of histlogy. I wanted to get some opinions on this > field vs. cytology. I am wondering which field is better for me. Any suggestions > or thoughts are welcome. Thanks!!! > > I remain yours truely, > > Candice Camille > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From candice_camille <@t> yahoo.com Wed Jan 26 14:09:32 2011 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Wed Jan 26 14:09:36 2011 Subject: [Histonet] (no subject) In-Reply-To: <4D407BF3.1050602@vneubert.com> References: <894015.28954.qm@web62202.mail.re1.yahoo.com> <4D407BF3.1050602@vneubert.com> Message-ID: <727634.33242.qm@web62208.mail.re1.yahoo.com> Yes, I just started working in a histology lab. Thats y I was asking this question. I heard about cytology and wanted to get some opinions as to who offered what! Just trying to keep the options open. I doubt you are on any blacklist. LOL!! I remain yours truely, Candice Camille ________________________________ From: V. Neubert To: histonet@lists.utsouthwestern.edu Sent: Wed, January 26, 2011 1:54:27 PM Subject: Re: [Histonet] (no subject) Is there a chance to go and see a lab which does histology? > Hello > > I am really new to the field of histlogy. I wanted to get some opinions on >this > > field vs. cytology. I am wondering which field is better for me. Any >suggestions > > or thoughts are welcome. Thanks!!! > > I remain yours truely, > > Candice Camille > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jan 26 14:15:29 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jan 26 14:15:36 2011 Subject: [Histonet] glass cleaner In-Reply-To: <6DBD71C31D7E444482E5D3DFBC202D260245D4DF@txhous1eb012.uson.usoncology.int> References: <6DBD71C31D7E444482E5D3DFBC202D260245D4DF@txhous1eb012.uson.usoncology.int> Message-ID: HISTOLOGY UNIVERSAL WASHING INSTRUCTIONS FOR GLASSWARE Alternative to Acid Cleaning Glassware 5% Clorox is a very inexpensive and readily available chemical treatment solution, which is an alternative to acid cleaning. All glassware used for special stains MUST be cleaned in the following manner. This method MUST be used when using glassware for special stains sensitive to metal, such as FE, GMS, Retic, Jones, Dieterle, Steiner and Warthin-Starry. 1. Make fresh each morning a 5% Clorox solution containing 1/2 Tbsp. per 5 gallons. of Liqui-Nox. 2. Soak all glassware in this solution for a minimum of 30 minutes to remove any stains, and then scrub with brush until all residue is removed. 3. For hard to get out stains, soak in a stronger solution of Clorox. 4. Rinse with copious amounts of tap water, followed by several changes of deionized water. All traces of Clorox must be removed. 5. Place glassware on drying rack or upside down to dry thoroughly. 6. Use caution that NO metal instruments touch glassware. This will contaminate certain solutions, which must be free of any metal, such as Iron and Silver Stains. 7. After glassware is dry, put parafilm or gauze around top so no airborne contaminate may enter. 8. Put glassware away. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Jan 26, 2011, at 11:42 AM, Johnson, Nacaela wrote: > Hello.... > > I am looking for suggestions on the best chemical cleaner for > glassware > that is used for special stains. Any ideas? > > Thanks, > > Nacaela Johnson > Histology Technician > KCCC Pathology > 12000 110th St., Ste. 400 > Overland Park, KS 66210 > Office: 913-234-0576 > Fax: 913-433-7639 > Email: Nacaela.Johnson@USOncology.com > > The contents of this electronic mail message and any > attachments are confidential, possibly privileged and intended for > the addressee(s) only.
Only the addressee(s) may read, > disseminate, retain or otherwise use this message. If received in > error, please immediately inform the sender and then delete this > message without disclosing its contents to anyone. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Wed Jan 26 14:42:00 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Jan 26 14:42:04 2011 Subject: [Histonet] (no subject) In-Reply-To: <894015.28954.qm@web62202.mail.re1.yahoo.com> References: <894015.28954.qm@web62202.mail.re1.yahoo.com> Message-ID: Of course as most of us would say...histology rocks!! Most histotechs also do cytology is most hospital settings. The only difference is that cytotechs actually have to screen the slides that the histo people prepare. I think that cytologists make slightly more money...but histology is way more fun!! I would say maybe try and go visit a lab that has both types of techs there and see what looks like it would fit you best. Good luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 26, 2011 1:47 PM To: Histonet Subject: [Histonet] (no subject) Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Wed Jan 26 14:50:17 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Wed Jan 26 14:53:36 2011 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: Back in the day when I was trying to decide which school to pick for my scholarship monies, I had a choice of the Med Tech program, the Cytology program or the Histotechnology program. I had absolutely no idea what Histology was but that is the program I choose. One of the best decisions I ever made. I have never, ever, ever been bored with this field. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Sent by: To histonet-bounces@ , lists.utsouthwest ern.edu cc Subject 01/26/2011 03:42 RE: [Histonet] (no subject) PM Of course as most of us would say...histology rocks!! Most histotechs also do cytology is most hospital settings. The only difference is that cytotechs actually have to screen the slides that the histo people prepare. I think that cytologists make slightly more money...but histology is way more fun!! I would say maybe try and go visit a lab that has both types of techs there and see what looks like it would fit you best. Good luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 26, 2011 1:47 PM To: Histonet Subject: [Histonet] (no subject) Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jan 26 15:10:48 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jan 26 15:10:55 2011 Subject: [Histonet] Thanks ORO Message-ID: Leave it to histonet to provide numerous responses! Thank you all for providing me with your formula's and techniques. I have some great information! Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From mucram11 <@t> comcast.net Wed Jan 26 15:12:50 2011 From: mucram11 <@t> comcast.net (mucram11@comcast.net) Date: Wed Jan 26 15:13:04 2011 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <305846243-1296076371-cardhu_decombobulator_blackberry.rim.net-1206571741-@bda383.bisx.prod.on.blackberry> I just sort of fell into Histology some 40 plus years ago and never looked back. I have left the field and always come back to it. I have done research and clinical and still love it. Pam Marcum UAMS Sent from my Verizon Wireless BlackBerry -----Original Message----- From: BSullivan@shorememorial.org Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 26 Jan 2011 15:50:17 To: Cc: ; Subject: RE: [Histonet] (no subject) Back in the day when I was trying to decide which school to pick for my scholarship monies, I had a choice of the Med Tech program, the Cytology program or the Histotechnology program. I had absolutely no idea what Histology was but that is the program I choose. One of the best decisions I ever made. I have never, ever, ever been bored with this field. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Sent by: To histonet-bounces@ , lists.utsouthwest ern.edu cc Subject 01/26/2011 03:42 RE: [Histonet] (no subject) PM Of course as most of us would say...histology rocks!! Most histotechs also do cytology is most hospital settings. The only difference is that cytotechs actually have to screen the slides that the histo people prepare. I think that cytologists make slightly more money...but histology is way more fun!! I would say maybe try and go visit a lab that has both types of techs there and see what looks like it would fit you best. Good luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 26, 2011 1:47 PM To: Histonet Subject: [Histonet] (no subject) Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Wed Jan 26 15:37:06 2011 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Jan 26 15:39:26 2011 Subject: [Histonet] =?iso-8859-7?q?TGF=E2_RII_?= Message-ID: Hi histonetters, I need suggestion and help with the TGF? RII (D-2): sc-17799 Ab, which should show a cytoplasmic staining. My problem is that I am getting a beautiful nuclear staining only. How I can fix this problem? What to pay attention on and what to change in the usual protocol? My AR is citrate buffer pH6. This is a monoclonal mouse Ab against human TGF? RII. This is only Ab that can work for me because it recognize only human TGF? RII and does not cross react with mouse. Thanks in advance, Naira From gankam <@t> googlemail.com Wed Jan 26 17:28:37 2011 From: gankam <@t> googlemail.com (Fabrice GANKAM) Date: Wed Jan 26 17:28:48 2011 Subject: [Histonet] RE: Looking for a product In-Reply-To: References: Message-ID: Was wandering of any of you guys have used either a anti MCP or IL1beta on rat brain. Thanks y'all From ross <@t> premierlab.com Wed Jan 26 17:46:45 2011 From: ross <@t> premierlab.com (Ross Benik) Date: Wed Jan 26 17:47:01 2011 Subject: [Histonet] dual fluorescent staining Message-ID: Hello everyone, I am working on a protocol for a dual fluorescent stain with two mouse antibodies (one is IgG1 and the other is IgG2a). We started by performing the two stains sequentially with a serum free protein blocking step in between, tagging one antibody with a 488 FITC labeled secondary and a 568 Cy3 secondary on the other (both goat anti mouse of course). With this method we are getting a lot of background staining so I was wondering if anyone who has experience in this sort of work if you have a recommendation of a proper blocking solution between the sequential stains? I've heard about using Rodent Block Rat from Biocare Medical or an un-conjugated goat anti mouse IgG secondary immunoglobulin but I would like to hear from you all as well! :-) Thanks, Ross From sonya.martin <@t> soton.ac.uk Thu Jan 27 03:36:29 2011 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Thu Jan 27 03:36:45 2011 Subject: [Histonet] Cytoplasmic staining with Ki67 in frozen sections Message-ID: <5F338719C0D0DE44BDFDD2B83D3FF7A1CB76395B27@UOS-CL-EX7-L3.soton.ac.uk> HI All, I've just been trying out a Ki67 antibody from Abcam (rabbit polyclonal) on some frozen mouse tissues. Staining looked ok in the colon with nuclear localisation in the cells of the basement membrane. However, what I'm really interested in is the spleen and the staining here was a mess! There was some nuclear staining especially in germinal centres and scattered around the white pulp but the red pulp contained clusters of cells with cytoplasmic staining only (I don't know if this is real staining, non-specific staining or an artefact), also there was bright particulate 'staining' all over the red pulp but not the white. Frozen sections were cut (10um), air dried overnight, fixed in acetone (10mins), blocked with 5% normal goat serum, Ki67 Ab for 2hrs room temp, secondary was goat anti rabbit AlexaFluor 488 (Invitrogen - clean on its own). I thought this Ki67 staining was going to be easy!! Any suggestions? Thanks Sonya From mab70 <@t> medschl.cam.ac.uk Thu Jan 27 06:16:56 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Thu Jan 27 06:18:56 2011 Subject: [Histonet] Beta Galactosidase antibody Message-ID: Hi Everyone, Does anyone have a favourite antibody for beta Galactosidase that they would be confident to recommend? Thanks a lot. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 From loveyadams40 <@t> yahoo.com Thu Jan 27 07:54:50 2011 From: loveyadams40 <@t> yahoo.com (lovetta adams) Date: Thu Jan 27 07:46:22 2011 Subject: [Histonet] Over process prostate biopsies tissue? Message-ID: <46107552-B301-4ADD-BD2E-CDE95E503B59@yahoo.com> Does anyone have any solutions on resolving over process prostate tissue. What different kinds of reagent and program for your processor that you used to help with the problem. Lovetta M Adams From AGleiberman <@t> cbiolabs.com Thu Jan 27 09:10:36 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Thu Jan 27 09:10:42 2011 Subject: [Histonet] RE: Beta Galactosidase antibody In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C0F004A@cbiolabs05.CBiolabs.local> Hi Margaert, Try this one from Aves lab: http://www.aveslab.com/products/epitope-tag-and-gfp-antibodies/b-gal-b-galactosidase-lacz-antibody/ I did not try them on paraffin, but it worked fine on formaldehyde-fixed cells and cryo-sections from formaldehyde-fixed embryonic tissue. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret Blount Sent: Thursday, January 27, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Beta Galactosidase antibody Hi Everyone, Does anyone have a favourite antibody for beta Galactosidase that they would be confident to recommend? Thanks a lot. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From AGleiberman <@t> cbiolabs.com Thu Jan 27 09:20:43 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Thu Jan 27 09:20:49 2011 Subject: [Histonet] RE: dual fluorescent staining In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C0F0072@cbiolabs05.CBiolabs.local> Hi Ross, Use sequential staining with monovalent anti-mouse Fab fragments (donkey or goat) from Jackson ImmunoResearch labeled with different fluorochromes - DyLight488 and DyLight594, for example. As blocking step I use 5% normal donkey serum. Usually it works fine. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross Benik Sent: Wednesday, January 26, 2011 6:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dual fluorescent staining Hello everyone, I am working on a protocol for a dual fluorescent stain with two mouse antibodies (one is IgG1 and the other is IgG2a). We started by performing the two stains sequentially with a serum free protein blocking step in between, tagging one antibody with a 488 FITC labeled secondary and a 568 Cy3 secondary on the other (both goat anti mouse of course). With this method we are getting a lot of background staining so I was wondering if anyone who has experience in this sort of work if you have a recommendation of a proper blocking solution between the sequential stains? I've heard about using Rodent Block Rat from Biocare Medical or an un-conjugated goat anti mouse IgG secondary immunoglobulin but I would like to hear from you all as well! :-) Thanks, Ross _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From mhale <@t> carisls.com Thu Jan 27 09:51:21 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Thu Jan 27 09:51:26 2011 Subject: [Histonet] HT Position Message-ID: <6F33D8418806044682A391273399860F06BF7FE0@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamargastro.com Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Thanks, Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From Sarah_Mack <@t> urmc.rochester.edu Thu Jan 27 10:08:24 2011 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Thu Jan 27 10:09:45 2011 Subject: [Histonet] NYSHS Call for Award Nominees Message-ID: Every year, the New York State Histotechnological Society presents our colleagues and peers with awards that recognize their achievements, commitment and professionalism. We are very excited to have 7 student scholarships, 1 mentoring award as well as 2 registration awards. Please visit our website to see the list of available awards as well as the requirements for eligibility. http://www.nyhisto.org/index.htm You must be a current NYSHS member in order to apply. The deadline for submission is Tuesday February 1, 2011. The Awards Committee, formed from the officers and board of directors of the society, carefully evaluate all nominees and supporting documentation submitted for the awards. Recipients are presented their award at the New York State Histotechnological Society annual spring meeting, May 14th in Albany, New York. If you have any questions, please feel free to contact me by email: Sarah_Mack@urmc.rochester.edu Good luck! Sarah Mack Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-1702 From liz <@t> premierlab.com Thu Jan 27 10:31:26 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jan 27 10:31:31 2011 Subject: [Histonet] Cytoplasmic staining with Ki67 in frozen sections In-Reply-To: <5F338719C0D0DE44BDFDD2B83D3FF7A1CB76395B27@UOS-CL-EX7-L3.soton.ac.uk> Message-ID: James I believe the nuclear staining pattern that you describe is normal for Ki-67 in the spleen, not sure about the cytoplasmic or particulate staining. Since you viewing with fluorescence the particulate staining may be autofluorescence of red blood cells. Normally our primary antibody incubations are only 30 minutes, 2 hours at RT seems a bit much. Did you try multiple dilutions of the primary? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James S. Sent: Thursday, January 27, 2011 2:36 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cytoplasmic staining with Ki67 in frozen sections HI All, I've just been trying out a Ki67 antibody from Abcam (rabbit polyclonal) on some frozen mouse tissues. Staining looked ok in the colon with nuclear localisation in the cells of the basement membrane. However, what I'm really interested in is the spleen and the staining here was a mess! There was some nuclear staining especially in germinal centres and scattered around the white pulp but the red pulp contained clusters of cells with cytoplasmic staining only (I don't know if this is real staining, non-specific staining or an artefact), also there was bright particulate 'staining' all over the red pulp but not the white. Frozen sections were cut (10um), air dried overnight, fixed in acetone (10mins), blocked with 5% normal goat serum, Ki67 Ab for 2hrs room temp, secondary was goat anti rabbit AlexaFluor 488 (Invitrogen - clean on its own). I thought this Ki67 staining was going to be easy!! Any suggestions? Thanks Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shshaw <@t> WPI.EDU Thu Jan 27 10:39:05 2011 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Thu Jan 27 10:39:09 2011 Subject: [Histonet] Rat Skin Message-ID: <223F1D19A67B3245ADED1F18858077E00506D8C3@S281.admin.wpi.edu> Can anybody share their protocol on processing rat skin I seem to be having a problem with it being under processed. I fix the tissue for 11hrs in zinc formalin and then run a 12 hour processing schedule the skin size is 2.5cmx1cm. Thanks, Sharon From Dolores_Fischer <@t> baxter.com Thu Jan 27 11:35:23 2011 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Thu Jan 27 11:35:29 2011 Subject: [Histonet] RE: Rat Skin In-Reply-To: <223F1D19A67B3245ADED1F18858077E00506D8C3@S281.admin.wpi.edu> References: <223F1D19A67B3245ADED1F18858077E00506D8C3@S281.admin.wpi.edu> Message-ID: The section of skin is too large. It should be no thicker than approximately 3mm. If you need to see the entire piece of skin, you should be able to submit multiple thin sections. A standard 12hr processing schedule works. I am not familiar with zinc formalin, but the standard fixation time for formalin is, minimally 24hrs, therefore I would guess your fixation time is also too short. Dolores -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon Sent: Thursday, January 27, 2011 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat Skin Can anybody share their protocol on processing rat skin I seem to be having a problem with it being under processed. I fix the tissue for 11hrs in zinc formalin and then run a 12 hour processing schedule the skin size is 2.5cmx1cm. Thanks, Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From c.m.vanderloos <@t> amc.uva.nl Thu Jan 27 12:07:57 2011 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Jan 27 12:08:07 2011 Subject: [Histonet] RE: Cytoplasmic staining with Ki67 in frozen sections Message-ID: <9006545b7c4924c.4d41c28d@amc.uva.nl> Dear Sonya,Acetone is an excellent fixative for many antibody to stain frozens, however nuclear antigens like Ki67 after acetone fixation get a bit fuzzy. I would strongly recommend to fix the slides 5 min at room temp in standard 4% buffered formalin, wash with PBS (or TBS) and start your IHC. You will see a much sharper staining result that is more confined to the nucleus.Hope this helps.Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 27 Jan 2011 09:36:29 +0000 From: "James S." Subject: [Histonet] Cytoplasmic staining with Ki67 in frozen sections To: "'histonet@lists.utsouthwestern.edu'" histonet@lists.utsouthwestern.edu HI All, I've just been trying out a Ki67 antibody from Abcam (rabbit polyclonal) on some frozen mouse tissues. Staining looked ok in the colon with nuclear localisation in the cells of the basement membrane. However, what I'm really interested in is the spleen and the staining here was a mess! There was some nuclear staining especially in germinal centres and scattered around the white pulp but the red pulp contained clusters of cells with cytoplasmic staining only (I don't know if this is real staining, non-specific staining or an artefact), also there was bright particulate 'staining' all over the red pulp but not the white. Frozen sections were cut (10um), air dried overnight, fixed in acetone (10mins), blocked with 5% normal goat serum, Ki67 Ab for 2hrs room temp, secondary was goat anti rabbit AlexaFluor 488 (Invitrogen - clean on its own). I thought this Ki67 staining was going to be easy!! Any suggestions? Thanks Sonya From bakevictoria <@t> gmail.com Thu Jan 27 12:18:07 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Jan 27 12:18:41 2011 Subject: [Histonet] RE: Rat Skin In-Reply-To: References: <223F1D19A67B3245ADED1F18858077E00506D8C3@S281.admin.wpi.edu> Message-ID: Hi What Dolores has said is correct. Zinc formalin is a good fixative for skin. Rodent skin has additional difficulties because of the fur and the keratin involved with the follicles/hair. The tissue sections should not be more than 3 -4 mm in thickness. Also having that large a piece of tissue in a cassette (length) can also contribute to some problems - not just with fixation but with how it is placed in the cassette for processing, then embedding and I'm sure with cutting. My experments with rodent (rat & mouse) had all fixation done off of the processor, on a shaker (belly dancer platform) for twenty four hours. Tissue was then washed in running tap water for thirty minutes and then placed in 70% ethanol for processing. The ratio of solution/tissue was 20X. No fixation was done on the processor as it would interfere with our other protocols. The actual processing time was 10.5 hours. I started with 70%, 80%, 2 - 95%, 2 - abs. etoh, 50/50 - abs/xylene, 2 - xylene and 4- changes paraffin. I was using an older Tissue tec processor with heat for reagents at 37 degrees C w/ vacuum and pressure. Watch your temperatures on the processor because if reagents become too hot it will dry out and damage the tissue. Paraffin was set at 65 degrees C using paraplast 2 also using vacuum and pressure. Not knowing what your protocol may be sort of leaves me giving you only generalities, if there is anything that I can assist you with further let me know. Good luck. Vikki On Thu, Jan 27, 2011 at 12:35 PM, Fischer, Dolores < Dolores_Fischer@baxter.com> wrote: > The section of skin is too large. It should be no thicker than > approximately 3mm. If you need to see the entire piece of skin, you should > be able to submit multiple thin sections. A standard 12hr processing > schedule works. I am not familiar with zinc formalin, but the standard > fixation time for formalin is, minimally 24hrs, therefore I would guess your > fixation time is also too short. > > Dolores > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon > Sent: Thursday, January 27, 2011 10:39 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Rat Skin > > Can anybody share their protocol on processing rat skin I seem to be having > a problem with it being under processed. I fix the tissue for 11hrs in zinc > formalin and then run a 12 hour processing schedule the skin size is > 2.5cmx1cm. > > Thanks, > Sharon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > The information transmitted is intended only for the person(s)or entity to > which it is addressed and may contain confidential and/or legally privileged > material. Delivery of this message to any person other than the intended > recipient(s) is not intended in any way to waive privilege or > confidentiality. Any review, retransmission, dissemination or other use of , > or taking of any action in reliance upon, this information by entities other > than the intended recipient is prohibited. If you receive this in error, > please contact the sender and delete the material from any computer. > > For Translation: > > http://www.baxter.com/email_disclaimer > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Thu Jan 27 12:27:04 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 27 12:27:10 2011 Subject: AW: [Histonet] Oil Red O for FS on Muscle In-Reply-To: <81CDB427-526C-445F-B80C-98E60585E3E2@yahoo.com> References: <81CDB427-526C-445F-B80C-98E60585E3E2@yahoo.com> Message-ID: <2137BEA44ECD42B7B73C327A15439830@dielangs.at> Hi Akemi, we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To prevent the precipitates on the slide, we stain in a plastic coplin jar with a screwed tap. The dye-precipitates accumulate on the ground. We take care not to mix them up while putting in the slide. So we need no filtering before staining. We only stain frozens of lung without fixation. I don?t know the formula of Oil Red O solution, but perhaps it can be handled like the sudan III. bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Akemi Allison Gesendet: Mittwoch, 26. J?nner 2011 18:16 An: histonet Betreff: [Histonet] Oil Red O for FS on Muscle Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am working with them with pH issues. The Oil Red O is very problematic. They are mixing before use and filtering with 42 Watman paper, but there is a lot of residual background on the slides. Also, they were not fixing the sections with 37% formaldehyde, even though the procedure calls for it. Could you share who you get the stain from also. Your assistance is greatly appreciated. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Jan 27 12:39:07 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jan 27 12:39:13 2011 Subject: [Histonet] Bacterial contamination Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4760C@nmdamailsvr.nmda.ad.nmsu.edu> My pathologist tells me I have floating bacteria in both special stains I did this morning (GMS and Gram); some slides have these floating critters and some don't. Because the only common solutions are those for processing and deparaffinization and because these bacteria appear to be floating above the plane of the tissue - I can't figure out where to start looking. My DI water is from a central source and is routinely quality-checked, and this is a new building (Sep. 2010) I don't want to blame that. Knowing full well that I am probably overlooking the obvious, I'm asking for help figuring this out. I need a Sputnik Moment. Thanks! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From BSullivan <@t> shorememorial.org Thu Jan 27 12:41:42 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Thu Jan 27 12:45:03 2011 Subject: [Histonet] Bacterial contamination In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4760C@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: One place you need to look is the floatation bath where you cut your slides. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Breeden, Sara" To Sent by: "Histonet" histonet-bounces@ lists.utsouthwest cc ern.edu "Ragsdale, John" Subject 01/27/2011 01:39 [Histonet] Bacterial contamination PM My pathologist tells me I have floating bacteria in both special stains I did this morning (GMS and Gram); some slides have these floating critters and some don't. Because the only common solutions are those for processing and deparaffinization and because these bacteria appear to be floating above the plane of the tissue - I can't figure out where to start looking. My DI water is from a central source and is routinely quality-checked, and this is a new building (Sep. 2010) I don't want to blame that. Knowing full well that I am probably overlooking the obvious, I'm asking for help figuring this out. I need a Sputnik Moment. Thanks! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> pathologyarts.com Thu Jan 27 12:56:58 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Thu Jan 27 12:57:07 2011 Subject: [Histonet] proper ventilation Message-ID: <001401cbbe53$f9ac6920$ed053b60$@tague@pathologyarts.com> I want to make some evaluations of my lab ventilation system and perhaps any upgrades that might be necessary. Does anyone have any recommendations for the Southern California area? Thank, Curt From Luis.Chiriboga <@t> nyumc.org Thu Jan 27 13:06:32 2011 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Thu Jan 27 13:07:07 2011 Subject: [Histonet] Paraffin Message-ID: HI all Just wanted to get peoples opinion's on the paraffin you use for processing animal tissues. I am interested in why you use a particular type, pros and cons. This query would apply to mouse and rat but I would also be interested in other species specific information if you have. You do not have to provide vendor info and feel free to email me off the net. If people are interested, I can compile and resend to the histonet Thanks L Luis Chiriboga Experimental Pathology Core Laboratory New York University School of Medicine ------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
From algranth <@t> email.arizona.edu  Thu Jan 27 13:21:37 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Thu Jan 27 13:21:49 2011
Subject: [Histonet] Paraffin
In-Reply-To: 
References: 
Message-ID: <11CF0114-AC89-4A10-B352-EA1F75E81FE6@email.arizona.edu>

I use Gem Cut Paraffin, Pink Sapphire. I like the way it ribbons and I'm using it in the processor and for embedding. I process/cut animal tissue. It comes in other colors but we are all girls here so we chose pink.

Andi Grantham



On Jan 27, 2011, at 12:06 PM, Chiriboga, Luis wrote:

> HI all
> Just wanted to get peoples opinion's on the paraffin you use for processing animal tissues.  I am interested in why you use a particular type, pros and cons. This query would apply to mouse and rat but I  would also be interested in other species specific information if you have.  You do not have to provide vendor info and feel free to email me off the net. If people are interested, I can compile and resend to the histonet
> 
> Thanks
> L
> 
> 
> Luis Chiriboga
> Experimental Pathology Core Laboratory
> New York University School of Medicine
> 
> 
> 
>
> > > ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
> ================================= > > > 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From sgoebel <@t> mirnarx.com  Thu Jan 27 13:23:56 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Thu Jan 27 13:24:00 2011
Subject: [Histonet] Paraffin
In-Reply-To: 
References: 
Message-ID: 

I have always liked paraplast.  It's good because you don't seem to have
as much tissue separation from the paraffin in the block.  It also seems
to hold up longer on the water bath to give sections a chance to
"de-wrinkle" themselves without having to pull so much with forceps.  It
also doesn't seem to be as oily as some other paraffins.
Just my two cents =)

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Chiriboga, Luis
Sent: Thursday, January 27, 2011 1:07 PM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Paraffin

HI all
Just wanted to get peoples opinion's on the paraffin you use for
processing animal tissues.  I am interested in why you use a particular
type, pros and cons. This query would apply to mouse and rat but I
would also be interested in other species specific information if you
have.  You do not have to provide vendor info and feel free to email me
off the net. If people are interested, I can compile and resend to the
histonet

Thanks
L


Luis Chiriboga
Experimental Pathology Core Laboratory
New York University School of Medicine
------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From POWELL_SA <@t> mercer.edu  Thu Jan 27 13:30:42 2011
From: POWELL_SA <@t> mercer.edu (Shirley A. Powell)
Date: Thu Jan 27 13:30:49 2011
Subject: [Histonet] GSH meeting - hotel deadline
Message-ID: <9BF995BC0E47744E9673A41486E24EE22DBAED0274@MERCERMAIL.MercerU.local>

Hi Georgia, Alabama, ALL histotechs,

The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line.  The invitation extends to any other states as well.  Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge.

The deadline for making hotel reservations is March 1, 2011  so that gives you a month to make your plans to attend, don't delay.  The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292.  Room rates start at $99 which includes Continental Breakfast and Admission to the Park.  For more information about things to do at Callaway click on the link here:   http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx

Our theme this year is "METAMORPHOSIS:  Transforming Histotechs."  The complete program can be downloaded from our website at this link:  www.histosearch.com/gsh then click on GSH symposium link at the bottom of the home page.  There you will find the complete program with registration form.  The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting.  If anyone has questions, please contact me for assistance.

Come TRANSFORM yourselves.


Shirley Powell
GSH Secretary



Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

From catherinesimonson <@t> gmail.com  Thu Jan 27 13:12:53 2011
From: catherinesimonson <@t> gmail.com (Catherine Simonson)
Date: Thu Jan 27 13:39:40 2011
Subject: [Histonet] Oil Red O for FS on Muscle
In-Reply-To: <2137BEA44ECD42B7B73C327A15439830@dielangs.at>
References: <81CDB427-526C-445F-B80C-98E60585E3E2@yahoo.com>
	<2137BEA44ECD42B7B73C327A15439830@dielangs.at>
Message-ID: 

Hello Akemi,

One thing though, how do you "mix" your oil red o?  just a stir bar?  I
found that if you don't shake it very vigorously for about 30 seconds before
filtering you will get that background staining.  Remember to shake it
(shaken, not stirred)

Catherine

On Thu, Jan 27, 2011 at 1:27 PM, Gudrun Lang  wrote:

> Hi Akemi,
> we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To
> prevent the precipitates on the slide, we stain in a plastic coplin jar
> with
> a screwed tap. The dye-precipitates accumulate on the ground. We take care
> not to mix them up while putting in the slide. So we need no filtering
> before staining. We only stain frozens of lung without fixation.
>
> I don?t know the formula of Oil Red O solution, but perhaps it can be
> handled like the sudan III.
>
> bye
> Gudrun
>
> -----Urspr?ngliche Nachricht-----
> Von: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Akemi
> Allison
> Gesendet: Mittwoch, 26. J?nner 2011 18:16
> An: histonet
> Betreff: [Histonet] Oil Red O for FS on Muscle
>
> Happy hump day!
>
> Does anyone have a good procedure for Oil Red O for FS on Muscle.
> The  procedure the lab I am working with is having a great deal of
> problems with their muscle biopsy panel.  I am trouble-shooting some
> of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am
> working with them with pH issues.
>
> The Oil Red O is very problematic.  They are mixing before use and
> filtering with 42 Watman paper, but there is a lot of residual
> background on the slides.  Also, they were not fixing the sections
> with 37% formaldehyde, even though the procedure calls for it.  Could
> you share who you get the stain from also.  Your assistance is
> greatly appreciated.
>
> Akemi
>
>
> Akemi Allison BS, HT (ASCP) HTL
> Director
> Phoenix Lab Consulting
> Tele: 408.335.9994
> E-Mail: akemiat3377@yahoo.com
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From sbreeden <@t> nmda.nmsu.edu  Thu Jan 27 14:42:06 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Thu Jan 27 14:42:11 2011
Subject: [Histonet] Bacterial Contamination
Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4760F@nmdamailsvr.nmda.ad.nmsu.edu>

Thanks to everyone that sent in their suggestions.  I especially liked
the one that suggested I inform all my pathologists that special stains
are unconstitutional.  Seriously, though, I'll give all your suggestions
a shot and see if I can get rid of the little rascals.  Thanks again!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From macveigh <@t> usc.edu  Thu Jan 27 15:09:20 2011
From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni)
Date: Thu Jan 27 15:08:05 2011
Subject: [Histonet] Oil Red O for FS on Muscle
Message-ID: <001801cbbe66$77375e10$65a61a30$@usc.edu>

Hi Akemi,

 

Order the stain from Electron Microscopy Sciences - cat # 26503-02.  It
comes as 250 ml solution and is a lot less expensive than some other
sources. I guess you can probably get it through VWR. 

 

Mix 3 parts of this stock with 2 parts dist. Water and let it stand for 10
min.

Filter through coarse filter paper. I have even used paper towel with no
problem. Anything finer will take forever to filter. 

Fix the sections with regular 10% NBF for few min. 3-5

Rinse slides in dist. Water few times

Working Oil -red-O for 10 min. I use the plastic containers for shipping
slides, because the stain will color the plastic and I can easily and
cheaply replace those, but you can wash them and reuse them multiple times.

70% alcohol - quick rinse to clear background

Dist. Water - 5 min

Mayers Hematoxylin 5 min

Tap water 10 min

Dist. water rinse or up to 1 min

Mount with Aqua Mount

 

Never had any problems. Normal muscle will have very, very tiny droplets. We
used it on rodent muscle, but it should work the same for human.

 

Good luck

Michelle Aloni 

 

Research Specialist

USC Keck School of Medicine

 

From histotech <@t> imagesbyhopper.com  Thu Jan 27 15:22:51 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Thu Jan 27 15:24:04 2011
Subject: [Histonet] Bacterial contamination
In-Reply-To: 
References: 
Message-ID: <776E5782-208D-4A0F-BFB4-044BDBE9DC73@imagesbyhopper.com>

Another place to check is the "holding" water that the slides rest in just prior to staining.  We run our slides to water on the H&E stainer and then transfer the rack to a "holding" water dish.  We bleach this dish nightly, as we have found it contaminated in the past.  We don't know what caused the contamination, but we have not it since the bleaching started!  ;o)

Good luck!

Michelle


On Jan 27, 2011, at 1:41 PM, BSullivan@shorememorial.org wrote:

> One place you need to look is the floatation bath where you cut your
> slides.
> 
> Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
> AP Supervisor
> Shore Memorial Hospital
> 609-653-3590
> 
> 
> Speak only well of people and you need never whisper
> 
> 
> 
>             "Breeden, Sara"                                               
>                          su.edu>                                                    To 
>             Sent by:                  "Histonet"                          
>             histonet-bounces@          
>             lists.utsouthwest                                          cc 
>             ern.edu                   "Ragsdale, John"                    
>                                                  
>                                                                   Subject 
>             01/27/2011 01:39          [Histonet] Bacterial contamination  
>             PM                                                            
> 
> 
> 
> 
> 
> 
> 
> 
> 
> My pathologist tells me I have floating bacteria in both special stains
> I did this morning (GMS and Gram); some slides have these floating
> critters and some don't.   Because the only common solutions are those
> for processing and deparaffinization and because these bacteria appear
> to be floating above the plane of the tissue - I can't figure out where
> to start looking.   My DI water is from a central source and is
> routinely quality-checked, and this is a new building (Sep. 2010)  I
> don't want to blame that. Knowing full well that I am probably
> overlooking the obvious, I'm asking for help figuring this out.  I need
> a Sputnik Moment.  Thanks!
> 
> 
> 
> Sally Breeden, HT(ASCP)
> 
> New Mexico Department of Agriculture
> 
> Veterinary Diagnostic Services
> 
> 1101 Camino de Salud NE
> 
> Albuquerque, NM  87102
> 
> 505-383-9278 (Histology Lab)
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From macveigh <@t> usc.edu  Thu Jan 27 15:25:44 2011
From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni)
Date: Thu Jan 27 15:24:29 2011
Subject: [Histonet] Oil Red O for FS on Muscle
Message-ID: <001d01cbbe68$c17975b0$446c6110$@usc.edu>

Few more things:

 

1.       The mixed/working solution is good for 1-2 hours only. If you
filter it for a long time, this might use the good working time and then you
have trouble, because it just won't work well for you. I just poor the stain
and the water in a glass cylinder and shake it. That's it. 

2.       After coverslipping, do not press on the coverslip to chase air
bubbles away or this might displace the lipid droplets from their original
place.

3.       Look at the slides as soon as possible. In few days, you will see
that the stain is changing into ugly black crystals and the longer you wait
the larger the crystals = have to repeat it all over again J

4.       To clean the glassware, spray All Purpose Cleaner, use a brush and
warm water

 

Michelle

From Beth.Fye <@t> HCAhealthcare.com  Thu Jan 27 15:29:08 2011
From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com)
Date: Thu Jan 27 15:29:13 2011
Subject: [Histonet] Cytology vs. Histology
Message-ID: <938F8EC5A524D34EB5796E23E52781D34EA91872A5@NADCWPMSGCMS05.hca.corpad.net>

I am a Cytotech that now functions as the Pathology Manager over both Histology and Cytology.  There is no easy answer and it totally depends on the work environment.  At our hospital, the Cytotechs also prep their work, and we do no GYN cytology.  So they spend an equal time between bench work and screening.  That is not true of many work environments.  As far as Histology, it also depends on your work environment.  Some Histotechs spend the whole day in front of the Microtome which can also be tough.  Histology requires a lot of hand/eye coordination to make a quality slide.  I think that is a skill that not everyone can do (well).  Both are very good careers, good luck with your decision.

Beth A. Fye, CT (ASCP)
Pathology Technical  Manager
HCA Richmond Hospital Laboratories
office:  (804)228-6564
fax: (804)323-8638





From KMB01 <@t> grh.org  Thu Jan 27 15:41:15 2011
From: KMB01 <@t> grh.org (Kathy M. Gorham)
Date: Thu Jan 27 15:41:20 2011
Subject: [Histonet] PROCESSORS
Message-ID: 

We are looking at purchasing a new processor.  I would like any feed
back negative and positive for the Shandon Excelsior ES vs. the VIP 6.
Thanks you.  You are always so helpful.  

Kathy Gorham H.T.


GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS
Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center
Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet

GRH Mission
We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services.


GRH Confidentiality Notice
This e-mail and any attached documents are for the intended recipient/s only
and should be protected against viewing by unauthorized persons. The information
herein may have been disclosed from records whose confidentiality is protected
by Federal and State Law. Federal regulations prohibit further distribution or
copying of this information without permission.  If you received this e-mail
transmission in error, please notify the sender immediately to arrange for return
or destruction of this information.

From AnthonyH <@t> chw.edu.au  Thu Jan 27 15:47:03 2011
From: AnthonyH <@t> chw.edu.au (Tony Henwood)
Date: Thu Jan 27 15:47:16 2011
Subject: [Histonet] RE: Bacterial contamination
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4760C@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B02E4760C@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <6D6BD1DE8A5571489398B392A38A7157060664@xmdb02.nch.kids>

Sally,

It is possible that the bugs are coming from the sectioning water bath. We found that if sections for microbiological staining were cut late in the day, the critters appeared over the slide (not up near the slide label). The controls, which were cut as a batch early morning did not contain the bugs.

The water bath, with extraneous nutrients from the sections, sit around ay 37oC or more and the bugs love it. Try taking a sample to your cytology dept and ask them to do a cytocentrifuge preparation (air-dried, giemsa (or similar) stained) and have a look.

We try and cut the sections for Gram, ZN and Fungi staining first thing in the morning to alleviate the problem.

Only a thought!

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Friday, 28 January 2011 5:39 AM
To: Histonet
Cc: Ragsdale, John
Subject: [Histonet] Bacterial contamination

My pathologist tells me I have floating bacteria in both special stains I did this morning (GMS and Gram); some slides have these floating
critters and some don't.   Because the only common solutions are those
for processing and deparaffinization and because these bacteria appear to be floating above the plane of the tissue - I can't figure out where
to start looking.   My DI water is from a central source and is
routinely quality-checked, and this is a new building (Sep. 2010)  I don't want to blame that. Knowing full well that I am probably overlooking the obvious, I'm asking for help figuring this out.  I need a Sputnik Moment.  Thanks!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From rjbuesa <@t> yahoo.com  Thu Jan 27 15:57:08 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Jan 27 15:57:12 2011
Subject: [Histonet] PROCESSORS
In-Reply-To: 
Message-ID: <186556.6534.qm@web65713.mail.ac4.yahoo.com>

I always recommend ANY VIP over?ANY other. My experience with several makes me do it.
Ren? J.

--- On Thu, 1/27/11, Kathy M. Gorham  wrote:


From: Kathy M. Gorham 
Subject: [Histonet] PROCESSORS
To: histonet@lists.utsouthwestern.edu
Date: Thursday, January 27, 2011, 4:41 PM


We are looking at purchasing a new processor.? I would like any feed
back negative and positive for the Shandon Excelsior ES vs. the VIP 6.
Thanks you.? You are always so helpful.? 

Kathy Gorham H.T.


GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS
Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center
Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet

GRH Mission
We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services.


GRH Confidentiality Notice
This e-mail and any attached documents are for the intended recipient/s only
and should be protected against viewing by unauthorized persons. The information
herein may have been disclosed from records whose confidentiality is protected
by Federal and State Law. Federal regulations prohibit further distribution or
copying of this information without permission.? If you received this e-mail
transmission in error, please notify the sender immediately to arrange for return
or destruction of this information.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From histowa13 <@t> hotmail.com  Thu Jan 27 17:48:26 2011
From: histowa13 <@t> hotmail.com (Debbie Nannenga)
Date: Thu Jan 27 17:48:30 2011
Subject: [Histonet] FOXP1
Message-ID: 


I'm wondering who in IHC land may be using FOXP1?  Are you using the polyclonal or the mouse monoclonal (which clone)?  Why do you prefer one over the other?  Any advvice will be more than welcome.
 
Thank you.
Debbie Nannenga, HTL(ASCP), QIHC
InCyte Pathology
Spokane Valley, WA 99216
dnannenga@incytepathology.com
  		 	   		  
From monimezme <@t> yahoo.com.ar  Thu Jan 27 19:29:05 2011
From: monimezme <@t> yahoo.com.ar (Mezme Moni)
Date: Thu Jan 27 19:29:09 2011
Subject: [Histonet] consultation Bodian-luxol
Message-ID: <337765.48362.qm@web161904.mail.bf1.yahoo.com>

Hello, 
I would like to know if someone has the protocol for doing Bodian-luxol in human 
brain. 


Thanks
Monica M. 


      
From member <@t> linkedin.com  Thu Jan 27 21:42:56 2011
From: member <@t> linkedin.com (Douglas Gregg via LinkedIn)
Date: Thu Jan 27 21:42:59 2011
Subject: [Histonet] Invitation to connect on LinkedIn
Message-ID: <807579203.2154700.1296186176514.JavaMail.app@ela4-bed81.prod>

LinkedIn
------------Douglas Gregg requested to add you as a connection on LinkedIn:
------------------------------------------

Jackie,

I'd like to add you to my professional network on LinkedIn.

- Douglas

Accept invitation from Douglas Gregg
http://www.linkedin.com/e/yvpgd1-gjgjs6ps-43/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1083845339_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPAPcPkQe3cUc359bQdNbj5ccP1lbPwQdj4Rc3sOcz8LrCBxbOYWrSlI/EML_comm_afe/

View invitation from Douglas Gregg
http://www.linkedin.com/e/yvpgd1-gjgjs6ps-43/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1083845339_3/3dvejcPdjgUcPwMckALqnpPbOYWrSlI/svi/

------------------------------------------

Why might connecting with Douglas Gregg be a good idea?

Have a question? Douglas Gregg's network will probably have an answer:
You can use LinkedIn Answers to distribute your professional questions to Douglas Gregg and your extended network. You can get high-quality answers from experienced professionals.

http://www.linkedin.com/e/yvpgd1-gjgjs6ps-43/ash/inv19_ayn/

 
-- 
(c) 2011, LinkedIn Corporation
From STACEY.LANGENBERG <@t> UCDENVER.EDU  Thu Jan 27 21:53:06 2011
From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey)
Date: Thu Jan 27 21:54:59 2011
Subject: [Histonet] PROCESSORS
In-Reply-To: <186556.6534.qm@web65713.mail.ac4.yahoo.com>
References: ,
	<186556.6534.qm@web65713.mail.ac4.yahoo.com>
Message-ID: <1F70FCBB6D4EC549B2ADF69B9F9EAC034D1071111E@STEAMBOAT.ucdenver.pvt>

I demoed the VIP 6 and it is a nice machine. I ended up purchasing the ASP 300 from Leica. We liked it so much we are purchasing a second one this week. My 2 cents worth!
"People are not an interruption of our business. People are our business."

Stacey Langenberg HT (ASCP) QIHC
Laboratory Manager
Histology/IF
CU Dermatopathology Consultants
1999 N. Fitzsimons Pkwy Suite 120
Aurora, CO 80045
Lab-720-859-3559  Fax- 303-344-0789  Office- 303-577-2303 Cell-970-405-7742
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Thursday, January 27, 2011 2:57 PM
To: histonet@lists.utsouthwestern.edu; Kathy M. Gorham
Subject: Re: [Histonet] PROCESSORS

I always recommend ANY VIP over ANY other. My experience with several makes me do it.
Ren? J.

--- On Thu, 1/27/11, Kathy M. Gorham  wrote:


From: Kathy M. Gorham 
Subject: [Histonet] PROCESSORS
To: histonet@lists.utsouthwestern.edu
Date: Thursday, January 27, 2011, 4:41 PM


We are looking at purchasing a new processor.  I would like any feed
back negative and positive for the Shandon Excelsior ES vs. the VIP 6.
Thanks you.  You are always so helpful.

Kathy Gorham H.T.


GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS
Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center
Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet

GRH Mission
We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services.


GRH Confidentiality Notice
This e-mail and any attached documents are for the intended recipient/s only
and should be protected against viewing by unauthorized persons. The information
herein may have been disclosed from records whose confidentiality is protected
by Federal and State Law. Federal regulations prohibit further distribution or
copying of this information without permission.  If you received this e-mail
transmission in error, please notify the sender immediately to arrange for return
or destruction of this information.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From reardonm <@t> unimelb.edu.au  Thu Jan 27 23:32:02 2011
From: reardonm <@t> unimelb.edu.au (Matt Reardon)
Date: Thu Jan 27 23:32:06 2011
Subject: [Histonet] TissueTek Processor
Message-ID: 

Hello,

I was wondering if anyone has a second-hand TissueTek E150 or similar that
they would like to sell?

Cheers,

Matt
From jorge.tornero <@t> gmail.com  Fri Jan 28 04:16:53 2011
From: jorge.tornero <@t> gmail.com (Jorge Tornero)
Date: Fri Jan 28 04:18:39 2011
Subject: [Histonet] Mercury free Gilson's fluid
Message-ID: 

Hello all,

Does anyone know about a Gilson's fluid formula which is mercury-free? I've
been searching and I've found a paper on it in Histo-Logic Vol VI, Num 4,
October 1976 by Carolyn A. Barsczcz who  reccomends the use oz zinc chloride
in substitution of mercury chloride in zenker type fixatives (Gilson's fluid
seems to be of this kind), but I haven't been able to find formulas. ?Any
suggestion?

Thank you very much

Jorge Tornero
From An.Eerdekens <@t> med.kuleuven.be  Fri Jan 28 04:18:02 2011
From: An.Eerdekens <@t> med.kuleuven.be (An Eerdekens)
Date: Fri Jan 28 04:18:41 2011
Subject: [Histonet] paraffin sections: white with airbubbles 
Message-ID: <85BD21DE1A6DE94D9A30A2F2C1D2328B3D547D230A@ICTS-S-EXC4-CA.luna.kuleuven.be>

Dear collegues,

I am experiencing following problem.

I have embedded hypothalamus tissue in paraffin using the following procedure:
-fixation in 4% paraformaldehyde for 48 hours, fixation of the tissue in 50% alcohol, next day in 70% alcohol,next day paraffin embedding.
During the paraffin embedding there was a short circuit and the machine did not work for any hours, so there was a delay in the process.

Now I am making slices of 5 micrometer, using the Microm HM 360.

The tissue is very white (looks like I am making much thicker sections) on the slices with airbells inside. I don't have an explanation for this and many samples are showing the same features.

Does someone know what might be the reason?

Thanks for the help.

Regards,

An Eerdekens
Laboratory of Intensive Care Medicine
Catholic University Leuven, Belgium

003216330518
From nto <@t> stowers.org  Fri Jan 28 07:15:47 2011
From: nto <@t> stowers.org (Thomas, Nancy)
Date: Fri Jan 28 07:17:11 2011
Subject: [Histonet] RE: paraffin sections: white with airbubbles 
In-Reply-To: <85BD21DE1A6DE94D9A30A2F2C1D2328B3D547D230A@ICTS-S-EXC4-CA.luna.kuleuven.be>
Message-ID: 

Hi An,
You did not mention a clearing step (xylene, or xylene substitute) between your alcohol and paraffin steps.  Maybe you just forgot to write it, but if you actually missed this step, your samples would be improperly processed leading to the problems you have.

Nancy Thomas
Stowers Institute for Medical Research
Kansas City, Mo

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of An Eerdekens
Sent: Friday, January 28, 2011 4:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] paraffin sections: white with airbubbles


Dear collegues,

I am experiencing following problem.

I have embedded hypothalamus tissue in paraffin using the following procedure: -fixation in 4% paraformaldehyde for 48 hours, fixation of the tissue in 50% alcohol, next day in 70% alcohol,next day paraffin embedding. During the paraffin embedding there was a short circuit and the machine did not work for any hours, so there was a delay in the process.

Now I am making slices of 5 micrometer, using the Microm HM 360.

The tissue is very white (looks like I am making much thicker sections) on the slices with airbells inside. I don't have an explanation for this and many samples are showing the same features.

Does someone know what might be the reason?

Thanks for the help.

Regards,

An Eerdekens
Laboratory of Intensive Care Medicine
Catholic University Leuven, Belgium

003216330518
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From kmerriam2003 <@t> yahoo.com  Fri Jan 28 07:44:15 2011
From: kmerriam2003 <@t> yahoo.com (Kim Merriam)
Date: Fri Jan 28 07:44:19 2011
Subject: [Histonet] OFF-TOPIC - stuff to do in New Orleans
Message-ID: <750568.91900.qm@web130121.mail.mud.yahoo.com>

Hi Guys,

I am sure?that there are New Orleans histonetters out there.? I am?going there 
in a couple of weeks for a few days with the kids (boys, age 9 and 14).? I was 
wondering if anyone could suggest some good places to go, for both kids and 
grownups (we?are going with other families and have built-in babysitters).

Thanks a bunch,
Kim
?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 


      
From MadaryJ <@t> MedImmune.com  Fri Jan 28 07:52:43 2011
From: MadaryJ <@t> MedImmune.com (Madary, Joseph)
Date: Fri Jan 28 07:52:50 2011
Subject: [Histonet] oct effect on hydroxyproline assays?
Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A13032A7FA8@MD1EV002.medimmune.com>

Do you know if people inflate with OCT and then successfully get protein
out of the tissue or look at collagen via hydroxyproline assay? I know
we have been able to extract mRNA  but not sure if the gycols in oct
would have any effect on getting protein out to look at collagen via the
assay mentioned. Many thanks to the histo geniusi out there.

 

Nick Madary, HT/HTL(ASCP)QIHC

Histology Mgr, Medimmune

301.398.6360(lab), 4745(vm),9745(fax)

 

"To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
original message and any accompanying documents from your system
immediately, without copying, reviewing or otherwise using them for any
purpose.  Thank you for your cooperation."

 

 




To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary.  This communication is expected to be read and/or used only by the individual(s) for whom it is intended.  If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose.  Thank you for your cooperation.
From member <@t> linkedin.com  Fri Jan 28 08:06:32 2011
From: member <@t> linkedin.com (James Dooley via LinkedIn)
Date: Fri Jan 28 08:06:36 2011
Subject: [Histonet] Invitation to connect on LinkedIn
Message-ID: <750438881.2696384.1296223592272.JavaMail.app@ela4-bed39.prod>

LinkedIn
------------James Dooley requested to add you as a connection on LinkedIn:
------------------------------------------

Jackie,

I'd like to add you to my professional network on LinkedIn.

- James

Accept invitation from James Dooley
http://www.linkedin.com/e/yvpgd1-gjh624wd-5a/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1084711877_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPsTe34NdPgUc359bR5NrCdve6NVbP0OejsUcjAOcz8LrCBxbOYWrSlI/EML_comm_afe/

View invitation from James Dooley
http://www.linkedin.com/e/yvpgd1-gjh624wd-5a/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1084711877_3/3dvdPsUcj4Td3wMckALqnpPbOYWrSlI/svi/ 
------------------------------------------

DID YOU KNOW LinkedIn can help you find the right service providers using recommendations from your trusted network? Using LinkedIn Services, you can take the risky guesswork out of selecting service providers by reading the recommendations of credible, trustworthy members of your network. 
http://www.linkedin.com/e/yvpgd1-gjh624wd-5a/svp/inv-25/

 
-- 
(c) 2011, LinkedIn Corporation
From nancy_schmitt <@t> pa-ucl.com  Fri Jan 28 08:47:18 2011
From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt)
Date: Fri Jan 28 08:47:32 2011
Subject: [Histonet] LEAN Processes/ new lab
Message-ID: <737BD0BF52F0744B96B74B61756AC06444D2666F2E@hestia.ad.pa-ucl.com>

Hi Histonetters-
I am looking for input, pros and cons or anything else you can offer.  We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes.  What would you do differently?  What would you do the same?  Are you in the tri-state (IA, WI, MN) area and open to visitors?

Thank you in advance for your thoughts-

Nancy Schmitt MLT, HT (ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA



NOTICE: This email may contain legally privileged information. The information
is for the use of only the intended recipient(s) even if addressed
incorrectly. If you are not the intended recipient, please notify the sender
that you have received it in error and then delete it along with any
attachments. Thank you.



From sfeher <@t> CMC-NH.ORG  Fri Jan 28 09:05:34 2011
From: sfeher <@t> CMC-NH.ORG (Feher, Stephen)
Date: Fri Jan 28 09:05:37 2011
Subject: [Histonet] LEAN Processes/ new lab
In-Reply-To: <737BD0BF52F0744B96B74B61756AC06444D2666F2E@hestia.ad.pa-ucl.com>
References: <737BD0BF52F0744B96B74B61756AC06444D2666F2E@hestia.ad.pa-ucl.com>
Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24669907@exchange.cmc-nh.org>

Hi Nancy,

We built a brand new pathology lab from the bottom up last year.  Drop
me an email off line or give me a call and we can discuss our lessons
learned etc.  I worked with a LEAN workflow specialist on the design and
equipment placement prior to the plans being approved.  We included the
architect in all of out discussions about this.  You can always come to
snowy New Hampshire and check it out yourself?


Steve

Stephen A. Feher, MS, SCT (ASCP)

Pathology Supervisor

Catholic Medical Center

100 McGregor Street

Manchester, NH 03102

603-663-6707

sfeher@cmc-nh.org



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Friday, January 28, 2011 9:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] LEAN Processes/ new lab

Hi Histonetters-
I am looking for input, pros and cons or anything else you can offer.
We are designing a new histology lab to move to and I am interested in
how others have handled this along with using LEAN processes.  What
would you do differently?  What would you do the same?  Are you in the
tri-state (IA, WI, MN) area and open to visitors?

Thank you in advance for your thoughts-

Nancy Schmitt MLT, HT (ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA



NOTICE: This email may contain legally privileged information. The
information is for the use of only the intended recipient(s) even if
addressed incorrectly. If you are not the intended recipient, please
notify the sender that you have received it in error and then delete it
along with any attachments. Thank you.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From settembr <@t> umdnj.edu  Fri Jan 28 09:12:33 2011
From: settembr <@t> umdnj.edu (Settembre, Dana)
Date: Fri Jan 28 09:12:41 2011
Subject: [Histonet] Any problems with Bond Max Heater Assembly (SSA)
In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC24669907@exchange.cmc-nh.org>
References: <737BD0BF52F0744B96B74B61756AC06444D2666F2E@hestia.ad.pa-ucl.com>
	<0908FC0A43B87A4FB60EDCCA06AABC24669907@exchange.cmc-nh.org>
Message-ID: 

Has anyone had any problems with the Leica Bond Max's
Heater assembly.  We have 2 Bonds that are less than
2 years old and one of the heater assembles went.
That is 10 less slides that we have to work with.
Thank you,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
973-972-5232

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen
Sent: Friday, January 28, 2011 10:06 AM
To: Nancy Schmitt; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] LEAN Processes/ new lab

Hi Nancy,

We built a brand new pathology lab from the bottom up last year.  Drop
me an email off line or give me a call and we can discuss our lessons
learned etc.  I worked with a LEAN workflow specialist on the design and
equipment placement prior to the plans being approved.  We included the
architect in all of out discussions about this.  You can always come to
snowy New Hampshire and check it out yourself?


Steve

Stephen A. Feher, MS, SCT (ASCP)

Pathology Supervisor

Catholic Medical Center

100 McGregor Street

Manchester, NH 03102

603-663-6707

sfeher@cmc-nh.org



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Friday, January 28, 2011 9:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] LEAN Processes/ new lab

Hi Histonetters-
I am looking for input, pros and cons or anything else you can offer.
We are designing a new histology lab to move to and I am interested in
how others have handled this along with using LEAN processes.  What
would you do differently?  What would you do the same?  Are you in the
tri-state (IA, WI, MN) area and open to visitors?

Thank you in advance for your thoughts-

Nancy Schmitt MLT, HT (ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA



NOTICE: This email may contain legally privileged information. The
information is for the use of only the intended recipient(s) even if
addressed incorrectly. If you are not the intended recipient, please
notify the sender that you have received it in error and then delete it
along with any attachments. Thank you.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From trathborne <@t> somerset-healthcare.com  Fri Jan 28 09:17:53 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Fri Jan 28 09:18:42 2011
Subject: [Histonet] Any problems with Bond Max Heater Assembly (SSA)
In-Reply-To: 
Message-ID: 

We have a Bond III that's about a year old. I'm also interested in the answer. What does Leica say is the normal replacement period for this item? Is it covered under your service contract?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Settembre, Dana
Sent: Friday, January 28, 2011 10:13 AM
To: 'Feher, Stephen'; Nancy Schmitt; histonet@lists.utsouthwestern.edu
Cc: Delia, Catherine
Subject: [Histonet] Any problems with Bond Max Heater Assembly (SSA)
Importance: High


Has anyone had any problems with the Leica Bond Max's
Heater assembly.  We have 2 Bonds that are less than
2 years old and one of the heater assembles went.
That is 10 less slides that we have to work with.
Thank you,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
973-972-5232

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen
Sent: Friday, January 28, 2011 10:06 AM
To: Nancy Schmitt; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] LEAN Processes/ new lab

Hi Nancy,

We built a brand new pathology lab from the bottom up last year.  Drop
me an email off line or give me a call and we can discuss our lessons
learned etc.  I worked with a LEAN workflow specialist on the design and
equipment placement prior to the plans being approved.  We included the
architect in all of out discussions about this.  You can always come to
snowy New Hampshire and check it out yourself?


Steve

Stephen A. Feher, MS, SCT (ASCP)

Pathology Supervisor

Catholic Medical Center

100 McGregor Street

Manchester, NH 03102

603-663-6707

sfeher@cmc-nh.org



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Friday, January 28, 2011 9:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] LEAN Processes/ new lab

Hi Histonetters-
I am looking for input, pros and cons or anything else you can offer.
We are designing a new histology lab to move to and I am interested in
how others have handled this along with using LEAN processes.  What
would you do differently?  What would you do the same?  Are you in the
tri-state (IA, WI, MN) area and open to visitors?

Thank you in advance for your thoughts-

Nancy Schmitt MLT, HT (ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA



NOTICE: This email may contain legally privileged information. The
information is for the use of only the intended recipient(s) even if
addressed incorrectly. If you are not the intended recipient, please
notify the sender that you have received it in error and then delete it
along with any attachments. Thank you.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
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Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.
From gvdobbin <@t> ihis.org  Fri Jan 28 09:38:43 2011
From: gvdobbin <@t> ihis.org (Greg Dobbin)
Date: Fri Jan 28 09:38:54 2011
Subject: [Histonet] Any problems with Bond Max Heater Assembly
	 (SSA)
In-Reply-To: 
References: 
	
Message-ID: <4D42AAC3020000C80000DEC1@smtp1.gov.pe.ca>

Hi Folks,
Someone from Leica should answer this question for you (Sara, are you listening in?), but I have a Bondmax and from time-to-time one of the heating pads will burnout. The other 9 positions continue to work. It used to be that the whole assembly was automatically replaced but now they manufacture them in such a way that an individual heater pad can be replaced. If you have a service contract this work will be covered. I think one or two heating pad errors per year could be expected - at least that has been my experience, but as I said, it doesn't slow us down much because the other 9 positions remain functional.
Greg
 
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
 
"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


>>> "Rathborne, Toni"  1/28/2011 11:17 AM >>>
We have a Bond III that's about a year old. I'm also interested in the answer. What does Leica say is the normal replacement period for this item? Is it covered under your service contract?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Settembre, Dana
Sent: Friday, January 28, 2011 10:13 AM
To: 'Feher, Stephen'; Nancy Schmitt; histonet@lists.utsouthwestern.edu
Cc: Delia, Catherine
Subject: [Histonet] Any problems with Bond Max Heater Assembly (SSA)
Importance: High


Has anyone had any problems with the Leica Bond Max's
Heater assembly.  We have 2 Bonds that are less than
2 years old and one of the heater assembles went.
That is 10 less slides that we have to work with.
Thank you,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
973-972-5232

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen
Sent: Friday, January 28, 2011 10:06 AM
To: Nancy Schmitt; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] LEAN Processes/ new lab

Hi Nancy,

We built a brand new pathology lab from the bottom up last year.  Drop
me an email off line or give me a call and we can discuss our lessons
learned etc.  I worked with a LEAN workflow specialist on the design and
equipment placement prior to the plans being approved.  We included the
architect in all of out discussions about this.  You can always come to
snowy New Hampshire and check it out yourself?


Steve

Stephen A. Feher, MS, SCT (ASCP)

Pathology Supervisor

Catholic Medical Center

100 McGregor Street

Manchester, NH 03102

603-663-6707

sfeher@cmc-nh.org



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Friday, January 28, 2011 9:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] LEAN Processes/ new lab

Hi Histonetters-
I am looking for input, pros and cons or anything else you can offer.
We are designing a new histology lab to move to and I am interested in
how others have handled this along with using LEAN processes.  What
would you do differently?  What would you do the same?  Are you in the
tri-state (IA, WI, MN) area and open to visitors?

Thank you in advance for your thoughts-

Nancy Schmitt MLT, HT (ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA



NOTICE: This email may contain legally privileged information. The
information is for the use of only the intended recipient(s) even if
addressed incorrectly. If you are not the intended recipient, please
notify the sender that you have received it in error and then delete it
along with any attachments. Thank you.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.

-------------------------
Statement of Confidentiality
This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system.



From Heidi.Hawthorne <@t> onassignment.com  Fri Jan 28 10:06:30 2011
From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne)
Date: Fri Jan 28 10:06:37 2011
Subject: [Histonet] Histotech with Hospital Experience
Message-ID: <26C4A3B38503BC4CBBF4D986C3BC9B83087EFE43E4@oasslcexm01.oaifield.onasgn.com>

Still have an immediate need in the East Bay of San Francisco for a histotech.  Must have hospital experience.  Hours can be flexible.  Ongoing contract position.  Great opportunity!

Contact me today for more details!


Heidi Hawthorne
Sr. Account Executive
On Assignment, Inc.
t: (510) 663-8622
c: (510) 435-7326
f: (866) 741-0805
Heidi.Hawthorne@onassignment.com
www.onassignment.com
NASDAQ: ASGN


People First.



From rjbuesa <@t> yahoo.com  Fri Jan 28 10:38:39 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Jan 28 10:38:43 2011
Subject: [Histonet] LEAN Processes/ new lab
In-Reply-To: <737BD0BF52F0744B96B74B61756AC06444D2666F2E@hestia.ad.pa-ucl.com>
Message-ID: <244177.67306.qm@web65703.mail.ac4.yahoo.com>

1-Make sure that your instruments are located in the same sequence as your procedure allowing the readily transfer of specimens between them.
2-Get a tissue processor and processing protocol?that will allow you to spend the same time preparing and processing the specimens, as the time it takes you to finish the slides.
3-Write cassettes and slides automatic, and reader for both in each cutting station.
4-Automate staining (all types) and coverslipping, and
5-Delegate all non technical tasks to assistants
Ren? J.
?

--- On Fri, 1/28/11, Nancy Schmitt  wrote:


From: Nancy Schmitt 
Subject: [Histonet] LEAN Processes/ new lab
To: "histonet@lists.utsouthwestern.edu" 
Date: Friday, January 28, 2011, 9:47 AM


Hi Histonetters-
I am looking for input, pros and cons or anything else you can offer.? We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes.? What would you do differently?? What would you do the same?? Are you in the tri-state (IA, WI, MN) area and open to visitors?

Thank you in advance for your thoughts-

Nancy Schmitt MLT, HT (ASCP)
Histology Coordinator
United Clinical Laboratories
Dubuque, IA



NOTICE: This email may contain legally privileged information. The information
is for the use of only the intended recipient(s) even if addressed
incorrectly. If you are not the intended recipient, please notify the sender
that you have received it in error and then delete it along with any
attachments. Thank you.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From relia1 <@t> earthlink.net  Fri Jan 28 10:51:21 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Fri Jan 28 10:51:37 2011
Subject: [Histonet] RELIA Histology Careers Bulletin  01/28/2011
Message-ID: <8D2A6442843B45E6BB3FB2A2F9A74D00@ownerf1abaad51>

Hi Histonetters!
 
Did you make a New Year?s Resolution? How is it going so far? 
Of course I resolved that I will try to eat right and exercise and I am
trying.     
 
But
my most important resolution is to help even more histo techs find
the right opportunity in the right place at the right time.
In 2010 I helped people make the move into a management role, relocate
closer to family, shorten their commute, move into research, utilize
their advanced degrees, get better salaries, shifts and benefits.
 
In 2011 I resolve to help even more people make the changes they want to
make in order to improve their career situations and ultimately their
quality of life.
 
I have a nationwide network of contacts with the premier employers of
histology professionals in clinical, research and biotech settings.  I
only work with full time permanent positions at companies that offer
excellent compensation, benefits programs and relocation assistance.
 
Here is a list of my current openings:
 
HISTOLOGY/MANAGEMENT
NC ? Histology Lab Manager ? Western NC 
NH- Histology Supervisor - Manchester, NH 
CA - Histology Lab Manager ? Central Valley of CA 
LA - Histology Supervisor ? Baton Rouge, LA 
 
HISTOTECHNICIANS/HISTOTECHNOLOGISTS
NC ? Western NC 
TX ? East Texas will consider new grads 
TX ? Austin ? Histotechnician/Histotechnologist 
TN ? Nashville Histotechnician. 
NY ? Long Island Histotechs NYS license req. 
NY ? Long Island Cyto prep techs NYS license req. 
NY ? Long Island Electron Microscopy Specialist NYS license req. 
NY-Orange/Rockland County NYS license req. 
NY ? Rochester Dermpath Histotech NYS license required 
WI ? Lead Histotech/Immunohistochemistry Specialist 
 
Of course I can?t put all the information about these opportunities in
an e-mail.  So if you or anyone you know might be interested in hearing
more about any of these positions or want help with a job search in
another area please contact me.  
Also remember I have new positions coming in on an almost daily basis so
if the area you desire is not listed don?t worry we can launch a
specific search for you for your preferred location remember it is free
of charge and it never hurts to look.  
 
So if you or anyone you know has resolved to make a job change this year
please let me know.  We can get started right away or we can map out a
strategy for when the timing is right.  Shoot me an e-mail at
relia1@earthlink.net or call me toll free at 866-607-3542.
 
There are a lot of recruiters out there right now trying to work with
histo techs and I appreciate your support and respect your needs.
Remember I offer over 25 years of experience as a recruiter and for over
9 years I have dedicated my practice solely to placing histology
professionals like you.  
 
Thanks again and I hope to hear from you soon, Thanks-Pam
 
Thank You!
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia
 
  
 
 
 
 

From c.tague <@t> pathologyarts.com  Fri Jan 28 11:23:02 2011
From: c.tague <@t> pathologyarts.com (Curt Tague)
Date: Fri Jan 28 11:23:14 2011
Subject: [Histonet] ventilation and exposure
Message-ID: <003101cbbf10$04e1a5f0$0ea4f1d0$@tague@pathologyarts.com>

Yesterday I asked for some direction on ventilation firms to evaluate my lab
and the fumes. I think I've got some decent leads but I have another quick
question, does any change the processor bottles, formalin, alcohols,
xylem/subs, inside a ventilated hood?

 

Thank for your input,

 

Curt 

 

From Nacaela.Johnson <@t> USONCOLOGY.COM  Fri Jan 28 11:23:00 2011
From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela)
Date: Fri Jan 28 11:24:04 2011
Subject: [Histonet] KC Dermpath job
Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D4E9@txhous1eb012.uson.usoncology.int>

I remember a post about a dermpath job opening.  Is this still
available?
 
Thanks,
 
Nacaela Johnson
Histology Technician
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson@USOncology.com
The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From liz <@t> premierlab.com Fri Jan 28 11:44:52 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jan 28 11:44:56 2011 Subject: [Histonet] ventilation and exposure In-Reply-To: <003101cbbf10$04e1a5f0$0ea4f1d0$@tague@pathologyarts.com> Message-ID: Curt We fill our bottles in the hood. They are emptied into a vented flammable cabinet that contains a 55 gallon drum. You know you can do your own chemical badges for both xylene and formalin. I like to run a couple TWA (8 hour) exposures and then I badge a lot of STEL (15 minutes) exposures on what I think are worse case scenarios or where I think individuals have exposure for a short period of time, like changing the tissue processor, or loading the tissue processor, changing the stainer, manually coverslipping, grossing in tissues, etc. If you are a small business OSHA has support services. They will come to your lab and perform an audit and review your safety practices (non punitive) and give you a report where they think you can improve. It was helpful to us. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Friday, January 28, 2011 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ventilation and exposure Yesterday I asked for some direction on ventilation firms to evaluate my lab and the fumes. I think I've got some decent leads but I have another quick question, does any change the processor bottles, formalin, alcohols, xylem/subs, inside a ventilated hood? Thank for your input, Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Fri Jan 28 11:46:05 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Fri Jan 28 11:49:28 2011 Subject: [Histonet] ventilation and exposure In-Reply-To: <003101cbbf10$04e1a5f0$0ea4f1d0$@tague@pathologyarts.com> Message-ID: Our 2 closed system processors are located under a hood. We do a remote drain and fill. This is all completed under the hood. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Curt Tague" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] ventilation and exposure 01/28/2011 12:23 PM Yesterday I asked for some direction on ventilation firms to evaluate my lab and the fumes. I think I've got some decent leads but I have another quick question, does any change the processor bottles, formalin, alcohols, xylem/subs, inside a ventilated hood? Thank for your input, Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri Jan 28 12:12:02 2011 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Jan 28 12:12:04 2011 Subject: [Histonet] UAMS Jobs for Histology In-Reply-To: <8D2A6442843B45E6BB3FB2A2F9A74D00@ownerf1abaad51> Message-ID: <211885077.1109099.1296238322732.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Sorry it would not go through for me on a regular post.? Pam Good Morning, We have two positions open in Little Rock, Arkansas at UAMS.? I will preface this by saying we are not able to pay relocation or other bonuses so please be aware of this before answering.? I will also state we are not allowed to use the services of out side placement services so please do not reply as I cannot (even if I want to) use your help.? If you are interested please send me a resume or CV and I will call you.? 1.? We need a Histology Supervisor with a minimum of 5 years supervisory experience and must? Ht or HTL certified.? This is a position that will require 60% bench work in the laboratory.? The salary levels are set by the state of Arkansas so the negotiating is limited.? I want to be completely honest in what we can do and mislead anyone.? 2. We need a histologist with IHC experience in our IHC department.? The person must be an HT, perferably with experience in this area.? Some rotation?with Histology is possible.? We currently have Ventana instruments so a familiarity with the equipment would be helpful.? General Histology experience preferred in the clinical area.? Pamela Marcum UAMS Anatomic Pathology Manager ----- Original Message ----- From: "Pam Barker" To: "Histonet" Sent: Friday, January 28, 2011 10:51:21 AM Subject: [Histonet] RELIA Histology Careers Bulletin ?01/28/2011 Hi Histonetters! ? Did you make a New Year?s Resolution? How is it going so far? Of course I resolved that I will try to eat right and exercise and I am trying. ? ? ? But?my most important resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2010 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get better salaries, shifts and benefits. ? In 2011 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately their quality of life. ? I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. ?I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. ? Here is a list of my current openings: ? HISTOLOGY/MANAGEMENT NC ? Histology Lab Manager ? Western NC NH- Histology Supervisor - Manchester, NH CA - Histology Lab Manager ? Central Valley of CA LA - Histology Supervisor ? Baton Rouge, LA ? HISTOTECHNICIANS/HISTOTECHNOLOGISTS NC ? Western NC TX ? East Texas will consider new grads TX ? Austin ? Histotechnician/Histotechnologist TN ? Nashville Histotechnician. NY ? Long Island Histotechs NYS license req. NY ? Long Island Cyto prep techs NYS license req. NY ? Long Island Electron Microscopy Specialist NYS license req. NY-Orange/Rockland County NYS license req. NY ? Rochester Dermpath Histotech NYS license required WI ? Lead Histotech/Immunohistochemistry Specialist ? Of course I can?t put all the information about these opportunities in an e-mail. ?So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. ? Also remember I have new positions coming in on an almost daily basis so if the area you desire is not listed don?t worry we can launch a specific search for you for your preferred location remember it is free of charge and it never hurts to look. ? ? So if you or anyone you know has resolved to make a job change this year please let me know. ?We can get started right away or we can map out a strategy for when the timing is right. ?Shoot me an e-mail at relia1@earthlink.net or call me toll free at 866-607-3542. ? There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 9 years I have dedicated my practice solely to placing histology professionals like you. ? ? Thanks again and I hope to hear from you soon, Thanks-Pam ? Thank You! ? Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: ? ? (407)353-5070 FAX: ? ? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com ?search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ? ?? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Fri Jan 28 12:12:20 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Jan 28 12:12:47 2011 Subject: [Histonet] Gilson's fluid Message-ID: <8CD8D2E0A3E221D-764-5891@web-mmc-d07.sysops.aol.com> Jorge Tornero asks about Gilson's fluid. The original Gilson's fluid was made up of Mercuric chloride 2 grams, glacial acetic acid 0.4ml, nitric acid 1.8 ml, 95% ethyl alcohol 10 ml, and distilled water 88ml. I don't know about usining zinc chloride in the formula Michael Titford USA Pathology Mobile AL USA From jeberry <@t> umich.edu Fri Jan 28 12:15:19 2011 From: jeberry <@t> umich.edu (Jan Berry) Date: Fri Jan 28 12:15:25 2011 Subject: [Histonet] fixation question for IHC Message-ID: <1BA205BB-22B9-4167-A36D-01B7F3F04A0B@umich.edu> Is there a difference between using paraformaldehyde and neutral buffered formalin when choosing a fixative for IHC? I would prefer to use formalin because of easier preparation, but am willing to put in the extra time to make fresh paraformaldehyde solution if there is a compelling reason. Jan Berry University of Michigan From brian <@t> detangleit.com Fri Jan 28 12:32:59 2011 From: brian <@t> detangleit.com (Brian Wood) Date: Fri Jan 28 12:33:17 2011 Subject: [Histonet] LEAN Processes/ new lab In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC24669907@exchange.cmc-nh.org> References: <737BD0BF52F0744B96B74B61756AC06444D2666F2E@hestia.ad.pa-ucl.com> <0908FC0A43B87A4FB60EDCCA06AABC24669907@exchange.cmc-nh.org> Message-ID: Hi Nancy, For labeling and identification our company provides a LEAN solution that allows you to print-on-demand using barcodes from cassettes, specimen containers, or requisition forms. This can eliminate batch processing, keyboard entry, handwriting, and adhesive labels from your workflow. Patient information is drawn from your LIS so there's always a single data source for everything that gets printed. The solution works for histology and cytology (including Hologic ThinPrep and BD Focal Point processors). The solution, overall, provides a LEAN, Zero Inventory, workflow with On-Demand printing at the microtome or cytology processor. I'd be happy to answer any questions or go into greater detail about how a process like this can work for you. Feel free to contact me if you would like to know more. Best Regards, Brian Brian Wood President Detangle IT Tel 516-594-9344 brian@detangleIT.com www.detangleIT.com From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, January 28, 2011 10:06 AM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] LEAN Processes/ new lab Hi Nancy, We built a brand new pathology lab from the bottom up last year. Drop me an email off line or give me a call and we can discuss our lessons learned etc. I worked with a LEAN workflow specialist on the design and equipment placement prior to the plans being approved. We included the architect in all of out discussions about this. You can always come to snowy New Hampshire and check it out yourself? Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, January 28, 2011 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEAN Processes/ new lab Hi Histonetters- I am looking for input, pros and cons or anything else you can offer. We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes. What would you do differently? What would you do the same? Are you in the tri-state (IA, WI, MN) area and open to visitors? Thank you in advance for your thoughts- Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This message scanned by Reflexion Total Control From AGleiberman <@t> cbiolabs.com Fri Jan 28 12:33:43 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Fri Jan 28 12:33:51 2011 Subject: [Histonet] fixation question for IHC In-Reply-To: <1BA205BB-22B9-4167-A36D-01B7F3F04A0B@umich.edu> References: <1BA205BB-22B9-4167-A36D-01B7F3F04A0B@umich.edu> Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C0F031A@cbiolabs05.CBiolabs.local> Jan, Depends on your material, further processing and antigens. Surprisingly, some antigens are better preserved after formaldehyde fixation than after buffered formalin (probably, due to high sensitivity of particular epitope to alcohol traces from standard formalin solution). I know at least one - mouse EpCAM epithelial marker almost negative after formalin and quite well stained after formaldehyde (rat monoclonal anti EpCAM antibody G8.8 from Developmental Studies Hybridoma Bank). Morphology and antigen staining on cryo-sections from formaldehyde-fixed mouse embryos (e11-e16) are slightly better than after formalin fixation (used the same 4-6h fixation time either with buffered formalin or with 4% formaldehyde on PBS prepared from 20% formaldehyde water solution from Electron Microscopy Sciences, following PBS wash and storage in PBS at +4 C). It seems, embryonic tissue is quite sensitive to coagulation induced by the presence of small amount of alcohol. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Berry Sent: Friday, January 28, 2011 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fixation question for IHC Is there a difference between using paraformaldehyde and neutral buffered formalin when choosing a fixative for IHC? I would prefer to use formalin because of easier preparation, but am willing to put in the extra time to make fresh paraformaldehyde solution if there is a compelling reason. Jan Berry University of Michigan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. 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From Ashley.Troutman <@t> Vanderbilt.Edu Fri Jan 28 12:43:03 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Jan 28 12:43:07 2011 Subject: [Histonet] Any problems with Bond Max Heater Assembly (SSA) Message-ID: <7B310892042DA74CB3590053F424CFE61387EF184C@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi, I see this issue from time to time. One thing to note, does the same heater stay out or do they jump around? If they jump around, this is probably due to an issue with the power for the instrument. Make sure all your line conditioners and battery backups are performing properly and this will fix it. If the same heater is going out, the heater is definitely bad and should be replaced. We have had a couple go out and it is a relatively easy fix, even though it can be a bit of a bother until they get replaced. Good luck! Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 From mhale <@t> carisls.com Fri Jan 28 12:50:06 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri Jan 28 12:50:10 2011 Subject: [Histonet] OHIO Message-ID: <6F33D8418806044682A391273399860F06C5EC17@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamar.com Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From Ronald.Houston <@t> nationwidechildrens.org Fri Jan 28 12:50:24 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jan 28 12:50:32 2011 Subject: [Histonet] Any problems with Bond Max Heater Assembly (SSA) In-Reply-To: <7B310892042DA74CB3590053F424CFE61387EF184C@ITS-HCWNEM06.ds.Vanderbilt.edu> References: <7B310892042DA74CB3590053F424CFE61387EF184C@ITS-HCWNEM06.ds.Vanderbilt.edu> Message-ID: We have had this problem occasionally too, but I must say, not with the same frequency we had with the Benchmark XT. Faulty heaters were replaced next working day. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Friday, January 28, 2011 1:43 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Any problems with Bond Max Heater Assembly (SSA) Hi, I see this issue from time to time. One thing to note, does the same heater stay out or do they jump around? If they jump around, this is probably due to an issue with the power for the instrument. Make sure all your line conditioners and battery backups are performing properly and this will fix it. If the same heater is going out, the heater is definitely bad and should be replaced. We have had a couple go out and it is a relatively easy fix, even though it can be a bit of a bother until they get replaced. Good luck! Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rsrichmond <@t> gmail.com Fri Jan 28 13:05:02 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Jan 28 13:05:06 2011 Subject: [Histonet] Re: Mercury free Gilson's fluid Message-ID: Gilson - whoever he may have been - the name is probably French, pronounced zheelSAW - apparently published both mercury and zinc forms of the fixative, more than a century ago. My source is the venerable Microtomist's Vade-Mecum, 11th ed. 1950, pages 40 and 43. The mercury formula can be conveniently accessed at http://stainsfile.info/StainsFile/prepare/fix/fixatives/gilson.htm Is there enough nitric acid in the brew to interfere with IHC? The zinc formula uses glacial acetic acid 5 mL 80% nitric acid 5 mL 80% alcohol 100 mL distilled water 300 mL "dry zinc chloride" 20 grams Published by Gilson in La Cellule, vi, 1890, page 122. Petrunkevitch's fixative is closely related, both historically and in composition. Bob Richmond Samurai Pathologist Knoxville TN From garret.t.miyamoto <@t> us.army.mil Fri Jan 28 14:05:54 2011 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Fri Jan 28 14:06:11 2011 Subject: [Histonet] Re: Processors In-Reply-To: <0LFP007XWSCAVEE0@mail23.us.army.mil> References: <0LFP007XWSCAVEE0@mail23.us.army.mil> Message-ID: Kathy, We use both processors and they work well and are happy with them. The only disadvantage I found with the Excelsior was the internal containers that hold the solutions. You cannot see the levels of the solutions. The level indicator on the screen is not always accurate. There is a function that you can use to check the fluid levels but it is time consuming since you have to pump the solution you are checking into the processing chamber. Garret Miyamoto Tripler Army Medical Center. ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Thursday, January 27, 2011 5:57 pm Subject: Histonet Digest, Vol 86, Issue 37 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Cytoplasmic staining with Ki67 in frozen sections > (C.M. van der Loos) > 2. Re: RE: Rat Skin (Victoria Baker) > 3. AW: [Histonet] Oil Red O for FS on Muscle (Gudrun Lang) > 4. Bacterial contamination (Breeden, Sara) > 5. Re: Bacterial contamination (BSullivan@shorememorial.org) > 6. proper ventilation (Curt Tague) > 7. Paraffin (Chiriboga, Luis) > 8. Re: Paraffin (Grantham, Andrea L - (algranth)) > 9. RE: Paraffin (sgoebel@mirnarx.com) > 10. GSH meeting - hotel deadline (Shirley A. Powell) > 11. Re: Oil Red O for FS on Muscle (Catherine Simonson) > 12. Bacterial Contamination (Breeden, Sara) > 13. Oil Red O for FS on Muscle (Michelle MacVeigh-Aloni) > 14. Re: Bacterial contamination (histotech@imagesbyhopper.com) > 15. Oil Red O for FS on Muscle (Michelle MacVeigh-Aloni) > 16. Cytology vs. Histology (Beth.Fye@HCAhealthcare.com) > 17. PROCESSORS (Kathy M. Gorham) > 18. RE: Bacterial contamination (Tony Henwood) > 19. Re: PROCESSORS (Rene J Buesa) > 20. FOXP1 (Debbie Nannenga) > 21. consultation Bodian-luxol (Mezme Moni) > 22. Invitation to connect on LinkedIn (Douglas Gregg via LinkedIn) > 23. RE: PROCESSORS (Langenberg, Stacey) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Thu, 27 Jan 2011 19:07:57 +0100 > From: "C.M. van der Loos" < > Subject: [Histonet] RE: Cytoplasmic staining with Ki67 in frozen > sections > To: sonya.martin@soton.ac.uk > Cc: histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=us-ascii > > Dear Sonya,Acetone is an excellent fixative for many antibody to stain frozens, however nuclear antigens like Ki67 after acetone fixation get a bit fuzzy. I would strongly recommend to fix the slides 5 min at room temp in standard 4% buffered formalin, wash with PBS (or TBS) and start your IHC. You will see a much sharper staining result that is more confined to the nucleus.Hope this helps.Cheers, ChrisChris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > Date: Thu, 27 Jan 2011 09:36:29 +0000 > From: "James S." < > Subject: [Histonet] Cytoplasmic staining with Ki67 in frozen sections > To: "'histonet@lists.utsouthwestern.edu'" > histonet@lists.utsouthwestern.edu > HI All, > > I've just been trying out a Ki67 antibody from Abcam (rabbit polyclonal) on some frozen mouse tissues. Staining looked ok in the colon with nuclear localisation in the cells of the basement membrane. However, what I'm really interested in is the spleen and the staining here was a mess! There was some nuclear staining especially in germinal centres and scattered around the white pulp but the red pulp contained clusters of cells with cytoplasmic staining only (I don't know if this is real staining, non-specific staining or an artefact), also there was bright particulate 'staining' all over the red pulp but not the white. > > Frozen sections were cut (10um), air dried overnight, fixed in acetone (10mins), blocked with 5% normal goat serum, Ki67 Ab for 2hrs room temp, secondary was goat anti rabbit AlexaFluor 488 (Invitrogen - clean on its own). > > I thought this Ki67 staining was going to be easy!! > > Any suggestions? > > Thanks > Sonya > > > ------------------------------ > > Message: 2 > Date: Thu, 27 Jan 2011 13:18:07 -0500 > From: Victoria Baker < > Subject: Re: [Histonet] RE: Rat Skin > To: "Shaw, Sharon" < > Cc: "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > Content-Type: text/plain; charset=ISO-8859-1 > > Hi > > What Dolores has said is correct. Zinc formalin is a good fixative for > skin. Rodent skin has additional difficulties because of the fur and the > keratin involved with the follicles/hair. The tissue sections should not be > more than 3 -4 mm in thickness. Also having that large a piece of tissue > in a cassette (length) can also contribute to some problems - not just with > fixation but with how it is placed in the cassette for processing, then > embedding and I'm sure with cutting. My experments with rodent (rat & > mouse) had all fixation done off of the processor, on a shaker (belly dancer > platform) for twenty four hours. Tissue was then washed in running tap > water for thirty minutes and then placed in 70% ethanol for processing. The > ratio of solution/tissue was 20X. No fixation was done on the processor as > it would interfere with our other protocols. The actual processing time was > 10.5 hours. I started with 70%, 80%, 2 - 95%, 2 - abs. etoh, 50/50 - > abs/xylene, 2 - xylene and 4- changes paraffin. I was using an older Tissue > tec processor with heat for reagents at 37 degrees C w/ vacuum and > pressure. Watch your temperatures on the processor because if reagents > become too hot it will dry out and damage the tissue. Paraffin was set at > 65 degrees C using paraplast 2 also using vacuum and pressure. > > Not knowing what your protocol may be sort of leaves me giving you only > generalities, if there is anything that I can assist you with further let me > know. > > Good luck. > > Vikki > > > > > On Thu, Jan 27, 2011 at 12:35 PM, Fischer, Dolores wrote: > > > The section of skin is too large. It should be no thicker than > > approximately 3mm. If you need to see the entire piece of skin, you should > > be able to submit multiple thin sections. A standard 12hr processing > > schedule works. I am not familiar with zinc formalin, but the standard > > fixation time for formalin is, minimally 24hrs, therefore I would guess your > > fixation time is also too short. > > > > Dolores > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon > > Sent: Thursday, January 27, 2011 10:39 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Rat Skin > > > > Can anybody share their protocol on processing rat skin I seem to be having > > a problem with it being under processed. I fix the tissue for 11hrs in zinc > > formalin and then run a 12 hour processing schedule the skin size is > > 2.5cmx1cm. > > > > Thanks, > > Sharon > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > The information transmitted is intended only for the person(s)or entity to > > which it is addressed and may contain confidential and/or legally privileged > > material. Delivery of this message to any person other than the intended > > recipient(s) is not intended in any way to waive privilege or > > confidentiality. Any review, retransmission, dissemination or other use of , > > or taking of any action in reliance upon, this information by entities other > > than the intended recipient is prohibited. If you receive this in error, > > please contact the sender and delete the material from any computer. > > > > For Translation: > > > > http://www.baxter.com/email_disclaimer > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 3 > Date: Thu, 27 Jan 2011 19:27:04 +0100 > From: "Gudrun Lang" < > Subject: AW: [Histonet] Oil Red O for FS on Muscle > To: "'Akemi Allison'" < > Cc: histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > Hi Akemi, > we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To > prevent the precipitates on the slide, we stain in a plastic coplin jar with > a screwed tap. The dye-precipitates accumulate on the ground. We take care > not to mix them up while putting in the slide. So we need no filtering > before staining. We only stain frozens of lung without fixation. > > I don?t know the formula of Oil Red O solution, but perhaps it can be > handled like the sudan III. > > bye > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Akemi > Allison > Gesendet: Mittwoch, 26. J?nner 2011 18:16 > An: histonet > Betreff: [Histonet] Oil Red O for FS on Muscle > > Happy hump day! > > Does anyone have a good procedure for Oil Red O for FS on Muscle. > The procedure the lab I am working with is having a great deal of > problems with their muscle biopsy panel. I am trouble-shooting some > of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am > working with them with pH issues. > > The Oil Red O is very problematic. They are mixing before use and > filtering with 42 Watman paper, but there is a lot of residual > background on the slides. Also, they were not fixing the sections > with 37% formaldehyde, even though the procedure calls for it. Could > you share who you get the stain from also. Your assistance is > greatly appreciated. > > Akemi > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Thu, 27 Jan 2011 11:39:07 -0700 > From: "Breeden, Sara" < > Subject: [Histonet] Bacterial contamination > To: "Histonet" < > Cc: "Ragsdale, John" < > Message-ID: > < > > Content-Type: text/plain; charset="US-ASCII" > > My pathologist tells me I have floating bacteria in both special stains > I did this morning (GMS and Gram); some slides have these floating > critters and some don't. Because the only common solutions are those > for processing and deparaffinization and because these bacteria appear > to be floating above the plane of the tissue - I can't figure out where > to start looking. My DI water is from a central source and is > routinely quality-checked, and this is a new building (Sep. 2010) I > don't want to blame that. Knowing full well that I am probably > overlooking the obvious, I'm asking for help figuring this out. I need > a Sputnik Moment. Thanks! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > > > ------------------------------ > > Message: 5 > Date: Thu, 27 Jan 2011 13:41:42 -0500 > From: BSullivan@shorememorial.org > Subject: Re: [Histonet] Bacterial contamination > To: "Breeden, Sara" < > Cc: Histonet <, "Ragsdale, John" > <, histonet-bounces@lists.utsouthwestern.edu > Message-ID: > < > > Content-Type: text/plain; charset=US-ASCII > > One place you need to look is the floatation bath where you cut your > slides. > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > AP Supervisor > Shore Memorial Hospital > 609-653-3590 > > > Speak only well of people and you need never whisper > > > > "Breeden, Sara" > < su.edu> To > Sent by: "Histonet" > histonet-bounces@ < > lists.utsouthwest cc > ern.edu "Ragsdale, John" > < > Subject > 01/27/2011 01:39 [Histonet] Bacterial contamination > PM > > > > > > > > > > My pathologist tells me I have floating bacteria in both special stains > I did this morning (GMS and Gram); some slides have these floating > critters and some don't. Because the only common solutions are those > for processing and deparaffinization and because these bacteria appear > to be floating above the plane of the tissue - I can't figure out where > to start looking. My DI water is from a central source and is > routinely quality-checked, and this is a new building (Sep. 2010) I > don't want to blame that. Knowing full well that I am probably > overlooking the obvious, I'm asking for help figuring this out. I need > a Sputnik Moment. Thanks! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 6 > Date: Thu, 27 Jan 2011 10:56:58 -0800 > From: "Curt Tague" < > Subject: [Histonet] proper ventilation > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > I want to make some evaluations of my lab ventilation system and perhaps any > upgrades that might be necessary. Does anyone have any recommendations for > the Southern California area? > > > > Thank, > > Curt > > > > > > > > ------------------------------ > > Message: 7 > Date: Thu, 27 Jan 2011 14:06:32 -0500 > From: "Chiriboga, Luis" < > Subject: [Histonet] Paraffin > To: "'Histonet@lists.utsouthwestern.edu'" > < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > HI all > Just wanted to get peoples opinion's on the paraffin you use for processing animal tissues. I am interested in why you use a particular type, pros and cons. This query would apply to mouse and rat but I would also be interested in other species specific information if you have. You do not have to provide vendor info and feel free to email me off the net. If people are interested, I can compile and resend to the histonet > > Thanks > L > > > Luis Chiriboga > Experimental Pathology Core Laboratory > New York University School of Medicine > > > > < > < > < > ------------------------------------------------------------< > This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.< > ================================= > < > < > < > > > ------------------------------ > > Message: 8 > Date: Thu, 27 Jan 2011 11:21:37 -0800 > From: "Grantham, Andrea L - (algranth)" < > Subject: Re: [Histonet] Paraffin > To: "Chiriboga, Luis" < > Cc: "Histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > I use Gem Cut Paraffin, Pink Sapphire. I like the way it ribbons and I'm using it in the processor and for embedding. I process/cut animal tissue. It comes in other colors but we are all girls here so we chose pink. > > Andi Grantham > > > > On Jan 27, 2011, at 12:06 PM, Chiriboga, Luis wrote: > > > HI all > > Just wanted to get peoples opinion's on the paraffin you use for processing animal tissues. I am interested in why you use a particular type, pros and cons. This query would apply to mouse and rat but I would also be interested in other species specific information if you have. You do not have to provide vendor info and feel free to email me off the net. If people are interested, I can compile and resend to the histonet > > > > Thanks > > L > > > > > > Luis Chiriboga > > Experimental Pathology Core Laboratory > > New York University School of Medicine > > > > > > > > < > > < > > < > > ------------------------------------------------------------< > > This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.< > > ================================= > > < > > < > > < > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Thu, 27 Jan 2011 13:23:56 -0600 > From: < > Subject: RE: [Histonet] Paraffin > To: <, < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > I have always liked paraplast. It's good because you don't seem to have > as much tissue separation from the paraffin in the block. It also seems > to hold up longer on the water bath to give sections a chance to > "de-wrinkle" themselves without having to pull so much with forceps. It > also doesn't seem to be as oily as some other paraffins. > Just my two cents =) > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Chiriboga, Luis > Sent: Thursday, January 27, 2011 1:07 PM > To: 'Histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Paraffin > > HI all > Just wanted to get peoples opinion's on the paraffin you use for > processing animal tissues. I am interested in why you use a particular > type, pros and cons. This query would apply to mouse and rat but I > would also be interested in other species specific information if you > have. You do not have to provide vendor info and feel free to email me > off the net. If people are interested, I can compile and resend to the > histonet > > Thanks > L > > > Luis Chiriboga > Experimental Pathology Core Laboratory > New York University School of Medicine > > > > < > < > < > ------------------------------------------------------------< > This email message, including any attachments, is for the sole use of > the intended recipient(s) and may contain information that is > proprietary, confidential, and exempt from disclosure under applicable > law. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you have received this email in error please notify the > sender by return email and delete the original message. Please note, the > recipient should check this email and any attachments for the presence > of viruses. The organization accepts no liability for any damage caused > by any virus transmitted by this email.< > ================================= > < > < > < > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Thu, 27 Jan 2011 14:30:42 -0500 > From: "Shirley A. Powell" < > Subject: [Histonet] GSH meeting - hotel deadline > To: "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > Hi Georgia, Alabama, ALL histotechs, > > The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge. > > The deadline for making hotel reservations is March 1, 2011 so that gives you a month to make your plans to attend, don't delay. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx > > Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The complete program can be downloaded from our website at this link: www.histosearch.com/gsh then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form. The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance. > > Come TRANSFORM yourselves. > > > Shirley Powell > GSH Secretary > > > > Shirley A. Powell, HT(ASCP)HTL, QIHC > Technical Director > Histology Curricular Support Laboratory > Mercer University School of Medicine > 1550 College Street > Macon, GA 31207 > 478-301-2374 Lab > 478-301-5489 Fax > > > > ------------------------------ > > Message: 11 > Date: Thu, 27 Jan 2011 14:12:53 -0500 > From: Catherine Simonson < > Subject: Re: [Histonet] Oil Red O for FS on Muscle > To: gu.lang@gmx.at > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > < > Content-Type: text/plain; charset=windows-1252 > > Hello Akemi, > > One thing though, how do you "mix" your oil red o? just a stir bar? I > found that if you don't shake it very vigorously for about 30 seconds before > filtering you will get that background staining. Remember to shake it > (shaken, not stirred) > > Catherine > > On Thu, Jan 27, 2011 at 1:27 PM, Gudrun Lang < wrote: > > > Hi Akemi, > > we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To > > prevent the precipitates on the slide, we stain in a plastic coplin jar > > with > > a screwed tap. The dye-precipitates accumulate on the ground. We take care > > not to mix them up while putting in the slide. So we need no filtering > > before staining. We only stain frozens of lung without fixation. > > > > I don?t know the formula of Oil Red O solution, but perhaps it can be > > handled like the sudan III. > > > > bye > > Gudrun > > > > -----Urspr?ngliche Nachricht----- > > Von: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Akemi > > Allison > > Gesendet: Mittwoch, 26. J?nner 2011 18:16 > > An: histonet > > Betreff: [Histonet] Oil Red O for FS on Muscle > > > > Happy hump day! > > > > Does anyone have a good procedure for Oil Red O for FS on Muscle. > > The procedure the lab I am working with is having a great deal of > > problems with their muscle biopsy panel. I am trouble-shooting some > > of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am > > working with them with pH issues. > > > > The Oil Red O is very problematic. They are mixing before use and > > filtering with 42 Watman paper, but there is a lot of residual > > background on the slides. Also, they were not fixing the sections > > with 37% formaldehyde, even though the procedure calls for it. Could > > you share who you get the stain from also. Your assistance is > > greatly appreciated. > > > > Akemi > > > > > > Akemi Allison BS, HT (ASCP) HTL > > Director > > Phoenix Lab Consulting > > Tele: 408.335.9994 > > E-Mail: akemiat3377@yahoo.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 12 > Date: Thu, 27 Jan 2011 13:42:06 -0700 > From: "Breeden, Sara" < > Subject: [Histonet] Bacterial Contamination > To: "Histonet" < > Cc: "Ragsdale, John" < > Message-ID: > < > > Content-Type: text/plain; charset="US-ASCII" > > Thanks to everyone that sent in their suggestions. I especially liked > the one that suggested I inform all my pathologists that special stains > are unconstitutional. Seriously, though, I'll give all your suggestions > a shot and see if I can get rid of the little rascals. Thanks again! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > > > ------------------------------ > > Message: 13 > Date: Thu, 27 Jan 2011 13:09:20 -0800 > From: "Michelle MacVeigh-Aloni" < > Subject: [Histonet] Oil Red O for FS on Muscle > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Hi Akemi, > > > > Order the stain from Electron Microscopy Sciences - cat # 26503-02. It > comes as 250 ml solution and is a lot less expensive than some other > sources. I guess you can probably get it through VWR. > > > > Mix 3 parts of this stock with 2 parts dist. Water and let it stand for 10 > min. > > Filter through coarse filter paper. I have even used paper towel with no > problem. Anything finer will take forever to filter. > > Fix the sections with regular 10% NBF for few min. 3-5 > > Rinse slides in dist. Water few times > > Working Oil -red-O for 10 min. I use the plastic containers for shipping > slides, because the stain will color the plastic and I can easily and > cheaply replace those, but you can wash them and reuse them multiple times. > > 70% alcohol - quick rinse to clear background > > Dist. Water - 5 min > > Mayers Hematoxylin 5 min > > Tap water 10 min > > Dist. water rinse or up to 1 min > > Mount with Aqua Mount > > > > Never had any problems. Normal muscle will have very, very tiny droplets. We > used it on rodent muscle, but it should work the same for human. > > > > Good luck > > Michelle Aloni > > > > Research Specialist > > USC Keck School of Medicine > > > > > > ------------------------------ > > Message: 14 > Date: Thu, 27 Jan 2011 16:22:51 -0500 > From: "histotech@imagesbyhopper.com" < > Subject: Re: [Histonet] Bacterial contamination > To: "BSullivan@shorememorial.org" < > Cc: Histonet <, > "histonet-bounces@lists.utsouthwestern.edu" > <, "Ragsdale, John" > < > Message-ID: < > Content-Type: text/plain; charset=us-ascii > > Another place to check is the "holding" water that the slides rest in just prior to staining. We run our slides to water on the H&E stainer and then transfer the rack to a "holding" water dish. We bleach this dish nightly, as we have found it contaminated in the past. We don't know what caused the contamination, but we have not it since the bleaching started! ;o) > > Good luck! > > Michelle > > > On Jan 27, 2011, at 1:41 PM, BSullivan@shorememorial.org wrote: > > > One place you need to look is the floatation bath where you cut your > > slides. > > > > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) > > AP Supervisor > > Shore Memorial Hospital > > 609-653-3590 > > > > > > Speak only well of people and you need never whisper > > > > > > > > "Breeden, Sara" > > <> su.edu> To > > Sent by: "Histonet" > > histonet-bounces@ < > > lists.utsouthwest cc > > ern.edu "Ragsdale, John" > > < > > Subject > > 01/27/2011 01:39 [Histonet] Bacterial contamination > > PM > > > > > > > > > > > > > > > > > > > > My pathologist tells me I have floating bacteria in both special stains > > I did this morning (GMS and Gram); some slides have these floating > > critters and some don't. Because the only common solutions are those > > for processing and deparaffinization and because these bacteria appear > > to be floating above the plane of the tissue - I can't figure out where > > to start looking. My DI water is from a central source and is > > routinely quality-checked, and this is a new building (Sep. 2010) I > > don't want to blame that. Knowing full well that I am probably > > overlooking the obvious, I'm asking for help figuring this out. I need > > a Sputnik Moment. Thanks! > > > > > > > > Sally Breeden, HT(ASCP) > > > > New Mexico Department of Agriculture > > > > Veterinary Diagnostic Services > > > > 1101 Camino de Salud NE > > > > Albuquerque, NM 87102 > > > > 505-383-9278 (Histology Lab) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 15 > Date: Thu, 27 Jan 2011 13:25:44 -0800 > From: "Michelle MacVeigh-Aloni" < > Subject: [Histonet] Oil Red O for FS on Muscle > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Few more things: > > > > 1. The mixed/working solution is good for 1-2 hours only. If you > filter it for a long time, this might use the good working time and then you > have trouble, because it just won't work well for you. I just poor the stain > and the water in a glass cylinder and shake it. That's it. > > 2. After coverslipping, do not press on the coverslip to chase air > bubbles away or this might displace the lipid droplets from their original > place. > > 3. Look at the slides as soon as possible. In few days, you will see > that the stain is changing into ugly black crystals and the longer you wait > the larger the crystals = have to repeat it all over again J > > 4. To clean the glassware, spray All Purpose Cleaner, use a brush and > warm water > > > > Michelle > > > > ------------------------------ > > Message: 16 > Date: Thu, 27 Jan 2011 15:29:08 -0600 > From: < > Subject: [Histonet] Cytology vs. Histology > To: < > Message-ID: > < > > Content-Type: text/plain; charset="us-ascii" > > I am a Cytotech that now functions as the Pathology Manager over both Histology and Cytology. There is no easy answer and it totally depends on the work environment. At our hospital, the Cytotechs also prep their work, and we do no GYN cytology. So they spend an equal time between bench work and screening. That is not true of many work environments. As far as Histology, it also depends on your work environment. Some Histotechs spend the whole day in front of the Microtome which can also be tough. Histology requires a lot of hand/eye coordination to make a quality slide. I think that is a skill that not everyone can do (well). Both are very good careers, good luck with your decision. > > Beth A. Fye, CT (ASCP) > Pathology Technical Manager > HCA Richmond Hospital Laboratories > office: (804)228-6564 > fax: (804)323-8638 > > > > > > > > ------------------------------ > > Message: 17 > Date: Thu, 27 Jan 2011 13:41:15 -0800 > From: "Kathy M. Gorham" < > Subject: [Histonet] PROCESSORS > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > We are looking at purchasing a new processor. I would like any feed > back negative and positive for the Shandon Excelsior ES vs. the VIP 6. > Thanks you. You are always so helpful. > > Kathy Gorham H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center > Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet > > GRH Mission > We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s only > and should be protected against viewing by unauthorized persons. The information > herein may have been disclosed from records whose confidentiality is protected > by Federal and State Law. Federal regulations prohibit further distribution or > copying of this information without permission. If you received this e-mail > transmission in error, please notify the sender immediately to arrange for return > or destruction of this information. > > > > ------------------------------ > > Message: 18 > Date: Thu, 27 Jan 2011 21:47:03 +0000 > From: Tony Henwood < > Subject: [Histonet] RE: Bacterial contamination > To: "'Breeden, Sara'" <, Histonet > < > Cc: "Ragsdale, John" < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Sally, > > It is possible that the bugs are coming from the sectioning water bath. We found that if sections for microbiological staining were cut late in the day, the critters appeared over the slide (not up near the slide label). The controls, which were cut as a batch early morning did not contain the bugs. > > The water bath, with extraneous nutrients from the sections, sit around ay 37oC or more and the bugs love it. Try taking a sample to your cytology dept and ask them to do a cytocentrifuge preparation (air-dried, giemsa (or similar) stained) and have a look. > > We try and cut the sections for Gram, ZN and Fungi staining first thing in the morning to alleviate the problem. > > Only a thought! > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara > Sent: Friday, 28 January 2011 5:39 AM > To: Histonet > Cc: Ragsdale, John > Subject: [Histonet] Bacterial contamination > > My pathologist tells me I have floating bacteria in both special stains I did this morning (GMS and Gram); some slides have these floating > critters and some don't. Because the only common solutions are those > for processing and deparaffinization and because these bacteria appear to be floating above the plane of the tissue - I can't figure out where > to start looking. My DI water is from a central source and is > routinely quality-checked, and this is a new building (Sep. 2010) I don't want to blame that. Knowing full well that I am probably overlooking the obvious, I'm asking for help figuring this out. I need a Sputnik Moment. Thanks! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 19 > Date: Thu, 27 Jan 2011 13:57:08 -0800 (PST) > From: Rene J Buesa < > Subject: Re: [Histonet] PROCESSORS > To: histonet@lists.utsouthwestern.edu, "Kathy M. Gorham" > < > Message-ID: < > Content-Type: text/plain; charset=iso-8859-1 > > I always recommend ANY VIP over?ANY other. My experience with several makes me do it. > Ren? J. > > --- On Thu, 1/27/11, Kathy M. Gorham < wrote: > > > From: Kathy M. Gorham < > Subject: [Histonet] PROCESSORS > To: histonet@lists.utsouthwestern.edu > Date: Thursday, January 27, 2011, 4:41 PM > > > We are looking at purchasing a new processor.? I would like any feed > back negative and positive for the Shandon Excelsior ES vs. the VIP 6. > Thanks you.? You are always so helpful.? > > Kathy Gorham H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center > Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet > > GRH Mission > We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s only > and should be protected against viewing by unauthorized persons. The information > herein may have been disclosed from records whose confidentiality is protected > by Federal and State Law. Federal regulations prohibit further distribution or > copying of this information without permission.? If you received this e-mail > transmission in error, please notify the sender immediately to arrange for return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 20 > Date: Thu, 27 Jan 2011 15:48:26 -0800 > From: Debbie Nannenga < > Subject: [Histonet] FOXP1 > To: < > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > > I'm wondering who in IHC land may be using FOXP1? Are you using the polyclonal or the mouse monoclonal (which clone)? Why do you prefer one over the other? Any advvice will be more than welcome. > > Thank you. > Debbie Nannenga, HTL(ASCP), QIHC > InCyte Pathology > Spokane Valley, WA 99216 > dnannenga@incytepathology.com > > > ------------------------------ > > Message: 21 > Date: Thu, 27 Jan 2011 17:29:05 -0800 (PST) > From: Mezme Moni < > Subject: [Histonet] consultation Bodian-luxol > To: Histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=utf-8 > > Hello, > I would like to know if someone has the protocol for doing Bodian-luxol in human > brain. > > > Thanks > Monica M. > > > > > ------------------------------ > > Message: 22 > Date: Fri, 28 Jan 2011 03:42:56 +0000 (UTC) > From: Douglas Gregg via LinkedIn < > Subject: [Histonet] Invitation to connect on LinkedIn > To: Jackie Smith < > Message-ID: > < > Content-Type: text/plain; charset=UTF-8 > > LinkedIn > ------------Douglas Gregg requested to add you as a connection on LinkedIn: > ------------------------------------------ > > Jackie, > > I'd like to add you to my professional network on LinkedIn. > > - Douglas > > Accept invitation from Douglas Gregg > http://www.linkedin.com/e/yvpgd1-gjgjs6ps-43/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1083845339_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPAPcPkQe3cUc359bQdNbj5ccP1lbPwQdj4Rc3sOcz8LrCBxbOYWrSlI/EML_comm_afe/ > > View invitation from Douglas Gregg > http://www.linkedin.com/e/yvpgd1-gjgjs6ps-43/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1083845339_3/3dvejcPdjgUcPwMckALqnpPbOYWrSlI/svi/ > > ------------------------------------------ > > Why might connecting with Douglas Gregg be a good idea? > > Have a question? Douglas Gregg's network will probably have an answer: > You can use LinkedIn Answers to distribute your professional questions to Douglas Gregg and your extended network. You can get high-quality answers from experienced professionals. > > http://www.linkedin.com/e/yvpgd1-gjgjs6ps-43/ash/inv19_ayn/ > > > -- > (c) 2011, LinkedIn Corporation > > ------------------------------ > > Message: 23 > Date: Thu, 27 Jan 2011 20:53:06 -0700 > From: "Langenberg, Stacey" < > Subject: RE: [Histonet] PROCESSORS > To: Rene J Buesa <, > "histonet@lists.utsouthwestern.edu" > <, "Kathy M. Gorham" < > Message-ID: > < > Content-Type: text/plain; charset="iso-8859-1" > > I demoed the VIP 6 and it is a nice machine. I ended up purchasing the ASP 300 from Leica. We liked it so much we are purchasing a second one this week. My 2 cents worth! > "People are not an interruption of our business. People are our business." > > Stacey Langenberg HT (ASCP) QIHC > Laboratory Manager > Histology/IF > CU Dermatopathology Consultants > 1999 N. Fitzsimons Pkwy Suite 120 > Aurora, CO 80045 > Lab-720-859-3559 Fax- 303-344-0789 Office- 303-577-2303 Cell-970-405-7742 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com] > Sent: Thursday, January 27, 2011 2:57 PM > To: histonet@lists.utsouthwestern.edu; Kathy M. Gorham > Subject: Re: [Histonet] PROCESSORS > > I always recommend ANY VIP over ANY other. My experience with several makes me do it. > Ren? J. > > --- On Thu, 1/27/11, Kathy M. Gorham < wrote: > > > From: Kathy M. Gorham < > Subject: [Histonet] PROCESSORS > To: histonet@lists.utsouthwestern.edu > Date: Thursday, January 27, 2011, 4:41 PM > > > We are looking at purchasing a new processor. I would like any feed > back negative and positive for the Shandon Excelsior ES vs. the VIP 6. > Thanks you. You are always so helpful. > > Kathy Gorham H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center > Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet > > GRH Mission > We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s only > and should be protected against viewing by unauthorized persons. The information > herein may have been disclosed from records whose confidentiality is protected > by Federal and State Law. Federal regulations prohibit further distribution or > copying of this information without permission. If you received this e-mail > transmission in error, please notify the sender immediately to arrange for return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 86, Issue 37 > **************************************** From jm.lapointe <@t> accellab.com Fri Jan 28 14:17:22 2011 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Fri Jan 28 14:17:25 2011 Subject: [Histonet] re: fixation question for IHC (Jan Berry) In-Reply-To: <201101282009.p0SK94Z6011301@gateway5.lastspam.com> References: <201101282009.p0SK94Z6011301@gateway5.lastspam.com> Message-ID: I think it is a very small minority of markers that would be sensitive to the methanol residues present in formalin. So it depends on what you plan on staining. For general purposes, I would always go with formalin, unless there is a proven need for formaldehyde. __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu?bec, Canada J7H 1N8 tel:? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ? ? ------------------------------ Message: 3 Date: Fri, 28 Jan 2011 13:15:19 -0500 From: Jan Berry Subject: [Histonet] fixation question for IHC To: histonet@lists.utsouthwestern.edu Message-ID: <1BA205BB-22B9-4167-A36D-01B7F3F04A0B@umich.edu> Content-Type: text/plain; charset=us-ascii Is there a difference between using paraformaldehyde and neutral buffered formalin when choosing a fixative for IHC? I would prefer to use formalin because of easier preparation, but am willing to put in the extra time to make fresh paraformaldehyde solution if there is a compelling reason. Jan Berry University of Michigan From djemge <@t> aol.com Fri Jan 28 15:06:07 2011 From: djemge <@t> aol.com (Donna Emge) Date: Fri Jan 28 15:06:42 2011 Subject: [Histonet] Best Ever Oil Red O Protocol Message-ID: <000301cbbf2f$2f14ede0$8d3ec9a0$@com> Ada Feldman sent me an article on Oil Red O staining and I wrote the following protocol for my lab based on the article. It works exceptionally well, frozen adrenal controls have brightly stained positive cortex and clear negative medulla. Best of all it has no precipitate like all the other methods. It also birefringent under polarizing filters. This is the best Oil Red O protocol anywhere! Our lab has tried several water based mounting mediums and the warm glycerin jelly works best. With glycerin jelly the stain and hematoxylin fades very little, even after a week. Also the cover glass doesn?t slide around because the jelly firms up when it cools. OIL RED O IN TRIETHYL PHOSPHATE From: Ada T. Feldman and Richard W. Dapson (1974) Medical Laboratory Technology 31, 335-341, RELATIVE EFFECTIVENESS OF VARIOUS SOLVENTS FOR OIL RED O, Department of Biology, University of Michigan-Flint, Flint, Michigan 48503, U.S.A. Triethyl Phosphate (Spectrum T2256, 500ML, CAS 78-40-0) VWR cat. # 700006-688 Oil Red O (Alfa Aesar CAS# 1320-06-5, 25G, C.I. 26125) VWR cat # AAA12989-14 Glycerin Jelly Mounting Medium (Electron Microscopy Sciences, Cat. # 17998-10, 100 ml) VWR cat# 17998-10 VWR Harris Hematoxylin (VWR Premium Stains, 95057-858) VWR cat # 95057-858 Lithium Carbonate (TCI America, 500 G) VWR cat TCL0224-500G Disposable Transfer pipettes VWR cat# 16001-180 VWR Superfrost Plus Microscope slides (VWR 25X75MM PK72) VWR cat# 48311-703 VWR? Micro Cover Glasses, Rectangular, No. 11/2 VWR cat# 48393-194 Solutions: 60% Triethyl Phosphate 600 ml Trithyl Phosphate 400 ml Distilled Water 0.5% Oil Red O in 60% Triethyl Phosphate 300 ml 60% Triethyl phosphate 1.5 gm Oil Red O Harris Hematoxylin Saturated Lithium Carbonate 18 gm lithium carbonate 1500 ml Distilled Water Technic: 1. Pre-warm Glycerin Jelly in 60?C waterbath 30mins prior to staining. 2. Bring frozen sections to room temperature. 3. 60% Triethyl Phosphate ? a few dips. 4. Stain sections in 0.5% Oil Red O, 15 to 20 minutes. 5. Rinse in water 2 minutes. 6. Counterstain in filtered Harris Hematoxylin, 2 minutes. 7. Blue in saturated Lithium Carbonate solution, 10 seconds 8. Rinse in water 5 minutes and hold in water. 9. Mount with warm Glycerin Jelly using a clean transfer pipette. Take care to avoid contaminating the pipette and Glycerin Jelly. Results: Fat ? Pink, Red, Bright Orange Nuclei - Blue Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 d-emge@northwestern.edu From jorge.tornero <@t> gmail.com Fri Jan 28 16:48:08 2011 From: jorge.tornero <@t> gmail.com (Jorge Tornero) Date: Fri Jan 28 16:48:16 2011 Subject: [Histonet] Re: Mercury free Gilson's fluid In-Reply-To: References: Message-ID: Hello, Robert, Michael and all those keeping an eye on this list, Thank you very much for your fast (instant, I'll say, taking into account our respective timezones) answer to my question. Best regards, Jorge Tornero Instituto Espa?ol de Oceanograf?a-C?diz Spain www.ieo.es 2011/1/28 Robert Richmond > Gilson - whoever he may have been - the name is probably French, > pronounced zheelSAW - apparently published both mercury and zinc forms > of the fixative, more than a century ago. My source is the venerable > Microtomist's Vade-Mecum, 11th ed. 1950, pages 40 and 43. > > The mercury formula can be conveniently accessed at > http://stainsfile.info/StainsFile/prepare/fix/fixatives/gilson.htm > > Is there enough nitric acid in the brew to interfere with IHC? > > The zinc formula uses > > glacial acetic acid 5 mL > 80% nitric acid 5 mL > 80% alcohol 100 mL > distilled water 300 mL > "dry zinc chloride" 20 grams > > Published by Gilson in La Cellule, vi, 1890, page 122. > > Petrunkevitch's fixative is closely related, both historically and in > composition. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From halloranrj <@t> msn.com Sat Jan 29 07:04:54 2011 From: halloranrj <@t> msn.com (Rosa and Jim) Date: Sat Jan 29 07:45:04 2011 Subject: [Histonet] Reichert-Jung 2040 Maintenance Message-ID: We use Bio-therm ? they are on the web under biotherm-inc I believe. They have a map of the country that shows service areas in case they will not come to Auburn you can find out who will. The guy that runs it is Bill Morris and I have known him for years ? he will help you out if they service lower Alabama. You can give him my name (Rosa from Cobb Hospital in Atlanta). Good luck, Rosa From mfisher <@t> ecrmc.org Sat Jan 29 09:59:08 2011 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Sat Jan 29 09:59:18 2011 Subject: [Histonet] Ownership of Slides/Blocks Message-ID: <3ACBB5D73A417547A01970CD3EB5509306CBC2A5@MAIL1.ecrmc.ci.el-centro.ca.us> Our lab receives cases for consultation from Mexico via the Oncology clinic. After we section, stain and perform all additional testing, the original blocks and slides should be sent back to the Oncology clinic to either be given back to the patient to return to Mexico or the Oncology clinic who requested send them back to the originating pathology lab. However, the Oncology clinic is refusing to so this so we would like to take ownership of the blocks and slides. Does anyone have a form that can be filled out or a letter that the originating Pathology lab in Mexico can sign giving us this ownership? Thanking you in advance for your help. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From jkiernan <@t> uwo.ca Sat Jan 29 23:02:24 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Jan 29 23:02:29 2011 Subject: [Histonet] re: fixation question for IHC (Jan Berry) Message-ID: I agree with Jean-Martin Lapointe. The small amount of methanol in a neutral buffered fixative made from formalin could have no fixative (coagulant) action. You need something approaching gin or vodka (40%v/v) to get ethanol to coagulate proteins and kill bacteria. Museums suggest vodka as a "spirit" preservative for specimens sent in by amateur naturalists. Very old formalin contains a little less formaldehyde, a little more methanol, and also some formic acid, but the acidity is dealt with when the pH of a neutral fixative is adjusted. Formalin stored in a cold place usually contains less than the 37%(w/w) or 40%(w/v) of formaldehyde stated on the label. The missing formaldehyde is is in the white precipitate (which is paraformaldehyde) in the bottom of the bottle. Fortunately for all who use formaldehyde as a fixative, the concentration probably does not much matter within the range 1% to 40%. I'm uncertain about the lower limit, but have encountered colleagues fixing animals brains in neat formalin for subsequent thick frozen sections to locate electrode placements. JF Walker's book "Formaldehyde" 3rd ed 1964, available as a reprint (Krieger 1975, ISBN 0882752189) cites many "preservative" uses of formaldehyde at concentrations lower than our customary 4%. Pretty good structural preservation for electron microscopy is possible with an intelligently formulated solution made from formalin with optimal salt concentrations and pH. Look up Carson FL, Martin JH & Lynn JA (1973) Am. J. Clin. Path. 59: 365-373. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Jean-Martin Lapointe Date: Friday, January 28, 2011 15:18 Subject: [Histonet] re: fixation question for IHC (Jan Berry) To: histonet@lists.utsouthwestern.edu > I think it is a very small minority of markers that would be > sensitive to the methanol residues present in formalin. So > it depends on what you plan on staining. For general purposes, I > would always go with formalin, unless there is a proven need for > formaldehyde. > __________________________________ > Jean-Martin Lapointe, DMV, MS, dACVP > Vice-President, Pathologie > AccelLAB Inc > 1635 Lionel-Bertrand, Boisbriand > Qu?bec, Canada J7H 1N8 > tel: 450-435-9482 ext.247 > fax: 450-435-4795 > jm.lapointe@accellab.com > > > > > > ------------------------------ > > Message: 3 > Date: Fri, 28 Jan 2011 13:15:19 -0500 > From: Jan Berry > Subject: [Histonet] fixation question for IHC > To: histonet@lists.utsouthwestern.edu > Message-ID: <1BA205BB-22B9-4167-A36D-01B7F3F04A0B@umich.edu> > Content-Type: text/plain; charset=us-ascii > > Is there a difference between using paraformaldehyde and neutral > buffered formalin when choosing a fixative for IHC? I > would prefer to use formalin because of easier preparation, but > am willing to put in the extra time to make fresh > paraformaldehyde solution if there is a compelling reason. > > Jan Berry > University of Michigan > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Milton.Gomez <@t> nyumc.org Sun Jan 30 08:00:58 2011 From: Milton.Gomez <@t> nyumc.org (Gomez, Milton) Date: Sun Jan 30 08:01:02 2011 Subject: [Histonet] Immunohistochemistry slides drying and baking protocol Message-ID: <4A53F9A1D7C2674FA4A6E650D703DDA5D40DEDAE@MSGWSDCPMB07.nyumc.org> Hello Histonetters, How and for how long and at what temperature is everyone air drying and baking slides for IHC staining?; specially for breast and cytology slides. Thank you very much in advance, MG ------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
From mab70 <@t> medschl.cam.ac.uk  Mon Jan 31 03:00:36 2011
From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount)
Date: Mon Jan 31 03:04:37 2011
Subject: [Histonet] fixation question for IHC
In-Reply-To: <1BA205BB-22B9-4167-A36D-01B7F3F04A0B@umich.edu>
References: <1BA205BB-22B9-4167-A36D-01B7F3F04A0B@umich.edu>
Message-ID: 

It is possible to freeze paraformaldehyde in aliquots, so you don't have
to keep on making it up repeatedly as we did in the old days. I have
also seen that you can buy Pure formaldehyde, but I don't remember who
the supplier was. It was in sealed ampoules and was 16% so you could
dilute it in your preferred buffer. I have to say that this was about 10
years ago!

Margaret

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan
Berry
Sent: 28 January 2011 18:15
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] fixation question for IHC

Is there a difference between using paraformaldehyde and neutral
buffered formalin when choosing a fixative for IHC?  I would prefer to
use formalin because of easier preparation, but am willing to put in the
extra time to make fresh paraformaldehyde solution if there is a
compelling reason.

Jan Berry
University of Michigan
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Melissa.Kuhnla <@t> chsli.org  Mon Jan 31 05:30:55 2011
From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa)
Date: Mon Jan 31 05:31:00 2011
Subject: [Histonet] Immunohistochemistry slides drying and baking protocol
In-Reply-To: <4A53F9A1D7C2674FA4A6E650D703DDA5D40DEDAE@MSGWSDCPMB07.nyumc.org>
References: <4A53F9A1D7C2674FA4A6E650D703DDA5D40DEDAE@MSGWSDCPMB07.nyumc.org>
Message-ID: 

Good Mornig,
We currently dry slides at room temp for 15 minutes then bake for 2
hours at 60 degrees.  Works well. Melissa

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez,
Milton
Sent: Sunday, January 30, 2011 9:01 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Immunohistochemistry slides drying and baking
protocol

Hello Histonetters,

How and for how long and at what temperature is everyone air drying and
baking slides for IHC staining?; specially for breast and cytology
slides.

Thank you very much in advance,
MG
------------------------------------------------------------
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================================= 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From Sarah_Mack <@t> urmc.rochester.edu  Mon Jan 31 06:17:54 2011
From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah)
Date: Mon Jan 31 06:19:11 2011
Subject: [Histonet] NYSHS Call for Award Nominees
Message-ID: 

Submission deadline extended until March 31, 2011!

Every year, the New York State Histotechnological Society presents our
colleagues and peers with awards that recognize their achievements,
commitment and professionalism.

We are very excited to have 7 student scholarships, 1 mentoring award as well as 2 registration awards.
Please visit our website to see the list of available awards as well as the requirements for eligibility.  http://www.nyhisto.org/index.htm
You must be a current NYSHS member in order to apply.  The deadline for submission has been extended to Thursday, March 31, 2011.

The Awards Committee, formed from the officers and board of directors of the
society, carefully evaluate all nominees and supporting documentation
submitted for the awards. Recipients are presented their award at the New
York State Histotechnological Society annual spring meeting, May 14th in Albany, New York.

If you have any questions, please feel free to contact me by email:
Sarah_Mack@urmc.rochester.edu

Good Luck!

Sarah Mack
University of Rochester Medical Center
Center for Musculoskeletal Research
601 Elmwood Avenue
Box 665
Rochester, NY 14642
(585)-273-1702
From Randi.Hayes <@t> horizonnb.ca  Mon Jan 31 08:26:41 2011
From: Randi.Hayes <@t> horizonnb.ca (Hayes, Randi (HorizonNB))
Date: Mon Jan 31 08:26:48 2011
Subject: [Histonet] IHC Reference Resources
Message-ID: 


Hi to everyone in Histoland,

I'm working on beefing up my resource library for IHC.  What are some
publications that you just couldn't go without?  Which provide the most
complete antibody information that is on the market today?

Thanks for your input!

Randi Hayes
Histology Supervisor, The Moncton Hospital
Horizon Health Network
Moncton, NB  Canada
randi.hayes@HorizonNB.ca
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From Melissa.Kuhnla <@t> chsli.org  Mon Jan 31 08:43:09 2011
From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa)
Date: Mon Jan 31 08:43:27 2011
Subject: [Histonet] IHC Reference Resources
In-Reply-To: 
References: 
Message-ID: 

I love the website nordiqc.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB)
Sent: Monday, January 31, 2011 9:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Reference Resources


Hi to everyone in Histoland,

I'm working on beefing up my resource library for IHC.  What are some
publications that you just couldn't go without?  Which provide the most
complete antibody information that is on the market today?

Thanks for your input!

Randi Hayes
Histology Supervisor, The Moncton Hospital
Horizon Health Network
Moncton, NB  Canada
randi.hayes@HorizonNB.ca
------- Horizon Health Network Disclaimer -------

This e-mail communication (including any or all attachments) is intended
only for the use of the person or entity to which it is addressed and may
contain confidential and/or privileged material. If you are not the intended
recipient of this e-mail, any use, review, retransmission, distribution,
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From MSHERWOOD <@t> PARTNERS.ORG  Mon Jan 31 08:52:11 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Mon Jan 31 08:52:19 2011
Subject: [Histonet] fixation question for IHC
In-Reply-To: 
References: <1BA205BB-22B9-4167-A36D-01B7F3F04A0B@umich.edu>
	
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5409@PHSXMB30.partners.org>

I routinely freeze Karnovsky's fixative (paraformaldehyde and glutaraldehyde
mix) in small aliquots.  When you thaw, just vigorously shake to make sure all
is in solution.  Have had no problems with fixation.

Peggy 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret Blount
Sent: Monday, January 31, 2011 4:01 AM
To: Jan Berry; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] fixation question for IHC

It is possible to freeze paraformaldehyde in aliquots, so you don't have
to keep on making it up repeatedly as we did in the old days. I have
also seen that you can buy Pure formaldehyde, but I don't remember who
the supplier was. It was in sealed ampoules and was 16% so you could
dilute it in your preferred buffer. I have to say that this was about 10
years ago!

Margaret

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan
Berry
Sent: 28 January 2011 18:15
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] fixation question for IHC

Is there a difference between using paraformaldehyde and neutral
buffered formalin when choosing a fixative for IHC?  I would prefer to
use formalin because of easier preparation, but am willing to put in the
extra time to make fresh paraformaldehyde solution if there is a
compelling reason.

Jan Berry
University of Michigan
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Histonet@lists.utsouthwestern.edu
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From relia1 <@t> earthlink.net  Mon Jan 31 09:10:02 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Mon Jan 31 09:10:07 2011
Subject: [Histonet] RELIA Histology Careers Bulletin 01/31/2011
Message-ID: <25D76F22DDAE4E9BA8B36F128384F2B9@ownerf1abaad51>

Hi Histonetters!!
In 2010 I helped people make the move into a management role, relocate
closer to family, shorten their commute, move into research, utilize their
advanced degrees get better salaries, shifts and benefits.
 
In 2011 I resolve to help even more people make the changes they want to
make in order to improve their career situations and ultimately their
quality of life.
 
I have a nationwide network of contacts with the premier employers of
histology professionals in clinical, research and biotech settings.  I only
work with full time permanent positions at companies that offer excellent
compensation, benefits programs and relocation assistance.
 
Here is a list of my current openings:
 
HISTOLOGY/MANAGEMENT
NC - Histology Lab Manager - Western NC 
NH- Histology Supervisor - Manchester, NH 
CA - Histology Lab Manager - Central Valley of CA 
LA - Histology Supervisor - Baton Rouge, LA 
 
HISTOTECHNICIANS/HISTOTECHNOLOGISTS
NC - Charlotte area 
NC - Western NC 
TX - East Texas will consider new grads 
TX - Austin - Histotechnician/Histotechnologist 
TN - Nashville Histotechnician. 
NY - Long Island Histotechs NYS license req. 
NY - Long Island Cyto prep techs NYS license req. 
NY - Long Island Electron Microscopy Specialist NYS license req. 
NY-Orange/Rockland County NYS license req. 
NY - Rochester Dermpath Histotech NYS license required 
WI - Lead Histotech/Immunohistochemistry Specialist 
 
Of course I can't put all the information about these opportunities in an
e-mail.  So if you or anyone you know might be interested in hearing more
about any of these positions or want help with a job search in another area
please contact me.  
 
Also remember I have new positions coming in on an almost daily basis so if
the area you desire is not listed don't worry we can launch a specific
search for you for your preferred location remember it is free of charge and
it never hurts to look.  
 
So if you or anyone you know has resolved to make a job change this year
please let me know.  We can get started right away or we can map out a
strategy for when the timing is right.  Shoot me an e-mail at
relia1@earthlink.net or call me toll free at 866*607*3542.
 
There are a lot of recruiters out there right now trying to work with histo
techs and I appreciate your support and respect your needs.  Remember I
offer over 25 years of experience as a recruiter and for over 9 years I have
dedicated my practice solely to placing histology professionals like you.  
 
Thanks again and I hope to hear from you soon, Thanks-Pam
 
Thank You!
  
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail:   relia1@earthlink.net 
  www.facebook.com  search Pam Barker RELIA
  www.linkedin.com/reliasolutions
  www.myspace.com/pamatrelia
  www.twitter.com/pamatrelia 

 
From alisha <@t> ka-recruiting.com  Mon Jan 31 10:41:37 2011
From: alisha <@t> ka-recruiting.com (Alisha Dynan)
Date: Mon Jan 31 10:40:45 2011
Subject: [Histonet] New York Histology Jobs
Message-ID: <210515092.1296492097727.JavaMail.cfservice@SL4APP1>



Dear Histonet Members,




I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm.  I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. 

 

I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions:

 

   * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus

   * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must


 

They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.  

If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future.  I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.  

To view some additional opportunities please visit our website at www.ka-recruiting.com. 



Sincerely,

 

Alisha (Taylor) Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

alisha@ka-recruiting.com

www.ka-recruiting.com

 






From gagnone <@t> KGH.KARI.NET  Mon Jan 31 10:56:59 2011
From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric)
Date: Mon Jan 31 10:57:12 2011
Subject: [Histonet] Immunohistochemistry slides drying and baking protocol
Message-ID: 

Hi Milton,
 
We've begun baking ALL IHC slides for 2 hours at 60 degrees C.  This also applies to unstained extra slides that may potentially used for IHC, including extra sections on breast cores, lymph node cores, cytology cell blocks etc.  No specific room temperature drying time before the oven though.  This has not resulted in any increase in washoffs.  We specify time for removal of individual baskets in the oven if oven is filling up, which it often does these days.  I should add we are using Ventana BenchMark XT, for which the recommendation initially was 30 mins at 37 degrees C before placing slides on the instrument.  
 
We've also started discarding unused unstained slides 3 months after they're cut.  Now if we can just reduce the number of unstained slides we cut that we don't need, that will be really great.
 
Hope this helps,
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada


From aonomic <@t> auburn.edu  Mon Jan 31 11:26:01 2011
From: aonomic <@t> auburn.edu (Michelle Aono)
Date: Mon Jan 31 11:26:20 2011
Subject: [Histonet] Dog Cartilage Safranin-O Stain
Message-ID: <4D469C49.5876.00D9.0@auburn.edu>

I am staining the joints of multiple species with Safranin-O/Fast
Green/Hematoxylin and consistently see a much weaker stain with the
SO/FG in dog cartilage.  At first I thought it had to do with the
thickness of the sections, but even as 6u it's still very light.  I'm
staining it at the same time as the other species so it's not variations
in staining time, solution age, etc...

Has anyone else seen this effect?  
Any ideas on how to fix this and why this is?  
Species proteoglycan compositional differences?

Thanks!

Histology Newbie,
:) Shelly

~~~~~~~~~~~~~~~
Michelle (Shelly) Aono
Histology Technician IV
CVM & AU Staff 
Advisory Council Representative
Auburn University
CVM-APP
212 Greene Hall, Auburn, AL 36832
(334) 844-5594


From foreightl <@t> gmail.com  Mon Jan 31 12:26:57 2011
From: foreightl <@t> gmail.com (Patrick Laurie)
Date: Mon Jan 31 12:27:04 2011
Subject: [Histonet] IHC Reference Resources
In-Reply-To: 
References: 
Message-ID: 

In my library is:
David Dabbs "Diagnostic Immunohistochemistry: Theranostic and Genomic
Applications" which is one of the best books,
Jules Elias "Immunohistopathology: a practical approach to diagnosis"
Taylor and Cote "Immunomicroscopy: a diagnostic tool for the surgical
pathologist"
Shan-Rong Shi "Antigen Retrieval Techniques: Immunohistochemistry and
Molecular Morphology"
C.M. van der Loos "Immunoenzyme multiple staining methods"
M Nadji et. al. "Efficient Tumor Immunohistochemistry"
J.M. Polak "Introduction to Immunocytochemistry"
And for research protocols:
L.C. Javois ed. "Immunocytochemical methods and protocols"

I think that the best are the first three books.

Good luck

On Mon, Jan 31, 2011 at 6:26 AM, Hayes, Randi (HorizonNB) <
Randi.Hayes@horizonnb.ca> wrote:

>
> Hi to everyone in Histoland,
>
> I'm working on beefing up my resource library for IHC.  What are some
> publications that you just couldn't go without?  Which provide the most
> complete antibody information that is on the market today?
>
> Thanks for your input!
>
> Randi Hayes
> Histology Supervisor, The Moncton Hospital
> Horizon Health Network
> Moncton, NB  Canada
> randi.hayes@HorizonNB.ca
>
>
>
------- Horizon Health Network Disclaimer -------

This e-mail > communication (including any or all attachments) is intended
only for the > use of the person or entity to which it is addressed and may
contain > confidential and/or privileged material. If you are not the > intended
recipient of this e-mail, any use, review, retransmission, > distribution,
dissemination, copying, printing, or other use of, or > taking of any action in
reliance upon this e-mail, is strictly > prohibited. If you have received this
e-mail in error, please contact the > sender and delete the original and any
copy of this e-mail and any > printout thereof, immediately. Your
co-operation is > appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) > s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un > organisme, et pourrait
comporter des renseignements privil?gi?s ou > confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est > interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de > diss?miner, de copier ou d'imprimer ce
courriel, d'agir en vous y fiant > ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent > courriel par erreur, pri?re de communiquer
avec l'exp?diteur et > d'?liminer l'original du courriel, ainsi que toute copie
?lectronique ou > imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de votre > collaboration.
>
> _______________________________________________
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie@cellnetix.com
From jclark <@t> pcnm.com  Mon Jan 31 12:44:28 2011
From: jclark <@t> pcnm.com (Joanne Clark)
Date: Mon Jan 31 12:44:33 2011
Subject: [Histonet] Hematology stainers
Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C014867C4@mail.pcnm.com>

Hi Histonetters, do any of you out there stain the peripheral, aspirate
and touch prep slides of bone marrows in the histo lab?  If you do, do
any of you use an automated stainer and if so, what kind of stainers are
being used?  I have quotes for a Wescor Aeorspray Stat Slide stainer, a
QuickSlide Plus Automated stainer and a Hematek 2000.  If anyone has had
experience good or bad for any of these stainers I would love to get
your feedback.

 

Thanks,

Joanne Clark, HT

Histology Supervisor

Pathology Consultants of New Mexico

From TJJ <@t> stowers.org  Mon Jan 31 13:19:32 2011
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Mon Jan 31 13:19:40 2011
Subject: [Histonet] Chromogenic ISH with riboprobes service
Message-ID: 

Can anyone recommend a laboratory or company that does ISH service using chromogenic detection with riboprobes we supply on slide mounted cryosections?

Thanks for your help, as always.

Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO


From flnails <@t> texaschildrens.org  Mon Jan 31 15:27:44 2011
From: flnails <@t> texaschildrens.org (Nails, Felton)
Date: Mon Jan 31 15:27:54 2011
Subject: [Histonet] RE: Hematology stainers
In-Reply-To: <0CDA5E1E01301F4880A8A7A8BCBDA39C014867C4@mail.pcnm.com>
References: <0CDA5E1E01301F4880A8A7A8BCBDA39C014867C4@mail.pcnm.com>
Message-ID: 

I thought I was the only histology lab that got stuck staining bone marrow smears.
I started with staining smears manually, then went to the hematek and finally discovered the Wescor stainer. The wescor stainer has really brought consistency to this area. The only potential problem is you have to keep up the maintenance, which is not extensive. I highly recommend the wescor and it is very nicely priced.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Monday, January 31, 2011 12:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hematology stainers

Hi Histonetters, do any of you out there stain the peripheral, aspirate and touch prep slides of bone marrows in the histo lab?  If you do, do any of you use an automated stainer and if so, what kind of stainers are being used?  I have quotes for a Wescor Aeorspray Stat Slide stainer, a QuickSlide Plus Automated stainer and a Hematek 2000.  If anyone has had experience good or bad for any of these stainers I would love to get your feedback.

 

Thanks,

Joanne Clark, HT

Histology Supervisor

Pathology Consultants of New Mexico

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From ross <@t> premierlab.com  Mon Jan 31 15:56:11 2011
From: ross <@t> premierlab.com (Ross Benik)
Date: Mon Jan 31 15:56:28 2011
Subject: [Histonet] Collagen X
Message-ID: 

Hello again everyone, 

 

First of all, just wanted to say thanks to everyone who provided advice
to my dual fluorescent stain question, it was all very helpful. Now, I
am wondering if anyone knows of a good positive control tissue for
Collagen Type X IHC staining? 

 

Thanks in advance,

Ross

 

 

From bbroders <@t> unlnotes.unl.edu  Mon Jan 31 16:10:51 2011
From: bbroders <@t> unlnotes.unl.edu (Bruce W Brodersen)
Date: Mon Jan 31 16:11:46 2011
Subject: [Histonet] Looking for a procedure
Message-ID: 


Looking for a procedure to embed tissue culture cells (speficially algae)
in agar for frozen sections.  Anyone have any?

Bruce W. Brodersen, DVM, PhD
University of Nebraska Veterinary Diagnostic Center
1900 N. 42nd Street
Lincoln, NE  68583-0907

voice (402) 472-1434
FAX (402 472-3094
From algranth <@t> email.arizona.edu  Mon Jan 31 17:20:55 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Mon Jan 31 17:21:06 2011
Subject: [Histonet] charge question (sorry)
Message-ID: <7271E459-D334-4654-94D2-4E7381611776@email.arizona.edu>

I hate to bring up charges again but I was asked to find out how much is being charged for a H&E on a slide that is already cut.
Thanks!

Andi Grantham
From DSiena <@t> statlab.com  Mon Jan 31 17:44:40 2011
From: DSiena <@t> statlab.com (Debra Siena)
Date: Mon Jan 31 17:44:47 2011
Subject: [Histonet] Marvin Hanna
Message-ID: 

Hi All,

Sorry to do this on the list but if Marvin Hanna is out there, could you contact me off line.  Thanks

Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
407 Interchange St. | McKinney, TX 75071
Direct: 972-436-1010  x229 | Fax: 972-436-1369
dsiena@statlab.com | www.statlab.com