[Histonet] Benchmark Problems

[Joe Nocito]

I would also see if Ventana can send you some test slides to test the 
heating. At my old lab, we had a problem with the heating plates. They 
repaired the entire platform. Just a thought.

Joe
----- Original Message ----- 
From: "Mark Tarango" <marktarango <@t> gmail.com>
To: "Dessoye, Michael J" <mjdessoye <@t> wvhcs.org>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 09, 2011 10:58 AM
Subject: Re: [Histonet] Benchmark Problems


> Hi Mike,
>
> Did you wash the bottles and rinse them and then pump the water through 
> the
> instrument before adding reagents and purging all again?  We had a problem
> like this about 2 weeks ago.  I think someone loaded the wrong bulk 
> reagent
> onto the instrument.  Cleaning it, purging with water and adding newly 
> made
> bulk reagents fixed the problem for us.
>
> Mark
>
> On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J 
> <mjdessoye <@t> wvhcs.org>wrote:
>
>> Hello,
>>
>> We started having a strange problem with our Ventana Benchmark XT.  Out 
>> of
>> the blue, our slides stopped staining.  With most antibodies we get no
>> reaction, some that were very strongly positive are now only very lightly
>> staining.  They do appear to counterstain OK.  Following Ventana's
>> recommendations we completely changed out all of our bulk fluids, purged 
>> the
>> lines, changed antibodies and DAB kits.  The last shot is some kind of
>> instrument issue that's not triggering an error.  Anyone ever see 
>> anything
>> like this?
>>
>> Mike Dessoye
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