From jkiernan <@t> uwo.ca Tue Feb 1 00:01:01 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Feb 1 00:01:06 2011 Subject: [Histonet] IHC Reference Resources In-Reply-To: References: Message-ID: Patrick, Your list is valuable advice: buy some books for the lab. This is the best possible investment. People who understand their work quickly solve problems and shoot troubles. I know most of the books in your list, and agree with your recommendations. BUT: The first item (Dabbs) has "Theranostic" in the title. This neologism is a bit much! Can we trust its inventor? John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Patrick Laurie Date: Monday, January 31, 2011 13:28 Subject: Re: [Histonet] IHC Reference Resources To: "Hayes, Randi (HorizonNB)" Cc: histonet@lists.utsouthwestern.edu > In my library is: > David Dabbs "Diagnostic Immunohistochemistry: Theranostic and Genomic > Applications" which is one of the best books, > Jules Elias "Immunohistopathology: a practical approach to diagnosis" > Taylor and Cote "Immunomicroscopy: a diagnostic tool for the surgical > pathologist" > Shan-Rong Shi "Antigen Retrieval Techniques: > Immunohistochemistry and > Molecular Morphology" > C.M. van der Loos "Immunoenzyme multiple staining methods" > M Nadji et. al. "Efficient Tumor Immunohistochemistry" > J.M. Polak "Introduction to Immunocytochemistry" > And for research protocols: > L.C. Javois ed. "Immunocytochemical methods and protocols" > > I think that the best are the first three books. > > Good luck > From louise.renton <@t> gmail.com Tue Feb 1 04:52:01 2011 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Feb 1 04:52:06 2011 Subject: [Histonet] in situ buffer?? Message-ID: Greetings, Oh mighty histonetters! As per previous posts I am re-embarking on doing in situ hybridization. I will be using (hopefully) DIG labelled oligo probes, and ultimately visualizing with DAB. My question is....are there a commercially available hybrizidation buffers? looking at the extensive list of ingredients it would be far easies (and cheaper) to get ready made stufff best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From sbreeden <@t> nmda.nmsu.edu Tue Feb 1 07:24:15 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Feb 1 07:24:19 2011 Subject: [Histonet] Histo Open Positions? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4761E@nmdamailsvr.nmda.ad.nmsu.edu> Can someone who might be "in the know" give me a ballpark estimate of how many open histologist/histotechnologist positions are available currently within the continental United States? I'm gathering baseline information for a little project I made up in-house. Thanks! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From akemiat3377 <@t> yahoo.com Tue Feb 1 07:54:26 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Feb 1 07:55:05 2011 Subject: [Histonet] calibration of pH meter Message-ID: <6D995FF4-53C4-4C83-8A41-73E848C0F3CA@yahoo.com> Good morning everyone, I am curious how frequently you all calibrate your pH meter, particularly when you make up solutions for muscle bx's? Do you do it daily, weekly, or monthly? I am trying to come up with an acceptable standard in a clinical histology lab, verses a biotech lab, which makes-up large quantities of reagents on a daily basis. Thank you in advance for your information, Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From JGarfield <@t> lifecell.com Tue Feb 1 09:00:11 2011 From: JGarfield <@t> lifecell.com (Garfield, Jacqueline) Date: Tue Feb 1 09:00:17 2011 Subject: [Histonet] RE: Collagen X In-Reply-To: References: Message-ID: <84439D447C5E9B44BECEA205781178300F3C558B@AMWPVEX01.kci.com> The tissue source for Collagen X is fetal cartilage. Jacqueline D. Garfield | Manager, Histology Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1182 Mobile 908.809.0495 Email jgarfield@ lifecell.com www.lifcell.com ? LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross Benik Sent: Monday, January 31, 2011 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Collagen X Hello again everyone, First of all, just wanted to say thanks to everyone who provided advice to my dual fluorescent stain question, it was all very helpful. Now, I am wondering if anyone knows of a good positive control tissue for Collagen Type X IHC staining? Thanks in advance, Ross _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vjp2105 <@t> columbia.edu Tue Feb 1 09:04:42 2011 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Tue Feb 1 09:04:45 2011 Subject: [Histonet] FISH Message-ID: Hi everyone, I was wondering if anyone would like to share their protocol for FISH on PFA fixed tissues? Any help would be hugely appreciated. Thanks so much. From alisha <@t> ka-recruiting.com Tue Feb 1 09:05:47 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Tue Feb 1 09:05:29 2011 Subject: [Histonet] Histology Jobs in Westchester County NY Message-ID: <1405877168.1296572747399.JavaMail.cfservice@SL4APP4> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on several open positions with a fast paced and expanding speciality laboratory in Westchester County. This company is provides transportation directly from the city to their lab. They are a speciality pathology company offering anatomic, molecular, digital, and local testing services. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus (1st or 3rd shift or Per Diem) * Experienced Histotech - NYS licensed histotech (2nd shift or Per Diem) * Cyto Prep Technician * Mycology Technician - Per Diem * PCR Tech - 1st shift They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From elciba <@t> hotmail.com Tue Feb 1 09:20:12 2011 From: elciba <@t> hotmail.com (ricky hachy) Date: Tue Feb 1 09:20:16 2011 Subject: [Histonet] Tissue Tek Pots Message-ID: Hello, I am looking for paraffin pots for a SAKURA/TISSUE TEK 4640 used or not working, any condition . ................. Thanks Ricky From leiker <@t> buffalo.edu Tue Feb 1 09:55:30 2011 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Feb 1 09:57:01 2011 Subject: [Histonet] in situ buffer?? In-Reply-To: References: Message-ID: <52ACE178CCDDCF5875BC54D8@CDYwxp1931.ad.med.buffalo.edu> I've made my own in situ buffer (for chromosome probes) using a simple recipe: 5ml 2xSSC pH6.8 5ml formamide (deionized) 1g dextran sulfate (DS) Sigma D6001 Warm the 2xSSC and add the DS to dissolve. Add formamide. Sterile filter. Aliquot and store at -20 C or lower. Hope this helps! Regards, Merced --On Tuesday, February 01, 2011 12:52 PM +0200 louise renton wrote: > Greetings, Oh mighty histonetters! > > As per previous posts I am re-embarking on doing in situ hybridization. I > will be using (hopefully) DIG labelled oligo probes, and ultimately > visualizing with DAB. > > My question is....are there a commercially available hybrizidation > buffers? looking at the extensive list of ingredients it would be far > easies (and cheaper) to get ready made stufff > > best regards > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From jcarpenter764 <@t> aol.com Tue Feb 1 10:05:55 2011 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Tue Feb 1 10:06:09 2011 Subject: [Histonet] Immunohistochemistry validation Message-ID: <8CD90410AA970AE-1CD8-4B34@webmail-d100.sysops.aol.com> Does anyone have any good advice or good articles I can read about validating immuno stains? thanks. Jennell = From gmartin <@t> marshallmedical.org Tue Feb 1 10:45:45 2011 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Feb 1 10:45:57 2011 Subject: [Histonet] Infectious waste Message-ID: <6ED9D4252F278841A0593D3D788AF24C0C3C49F4@mailsvr.MARSHMED.local> We are wondering if paraffin block shavings are considered infectious waste. From jkiernan <@t> uwo.ca Tue Feb 1 10:49:33 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Feb 1 10:49:42 2011 Subject: [Histonet] calibration of pH meter Message-ID: As a student I was told to calibrate the pH meter every time it was used (which was about twice a day at the time), using standard buffers at pH4.0 and 7.0. Nearly 50 years later this is still good advice, and modern meters are much less trouble to calibrate than older ones. If you have to make frequent measurements with closely similar solutions, you might get away with calibrating twice a day. It's the electrode, not the meter, that changes with time. All pH electrodes deteriorate with time, use and (especially) neglect. A slow response indicates that a new electrode is needed. Wikipedia has a nice article about pH meters, and there's a web site http://www.ph-meter.info/pH-electrode-calibration that gives advice about calibrating older and newer instruments. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Akemi Allison Date: Tuesday, February 1, 2011 8:56 Subject: [Histonet] calibration of pH meter To: histonet > Good morning everyone, > > I am curious how frequently you all calibrate your pH meter, > particularly when you make up solutions for muscle bx's? > Do you do it daily, weekly, or monthly? I am trying to > come up with an acceptable standard in a clinical histology lab, > verses a biotech lab, which makes-up large quantities of > reagents on a daily basis. > > Thank you in advance for your information, > Akemi > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jon.St.Onge <@t> dako.com Tue Feb 1 10:51:51 2011 From: Jon.St.Onge <@t> dako.com (Jon St.Onge) Date: Tue Feb 1 10:52:00 2011 Subject: [Histonet] calibration of pH meter In-Reply-To: <6D995FF4-53C4-4C83-8A41-73E848C0F3CA@yahoo.com> References: <6D995FF4-53C4-4C83-8A41-73E848C0F3CA@yahoo.com> Message-ID: <8B07D141BCDE434285DC12B3290E3FB30586F7AB@exbackca.caus.dako.net> Akemi, We calibrate our pH meters daily using standards of pH 4.0, pH 7.0, and pH 10.0 to cover the range of our testing. Check the owner's manual for your pH meter and follow the calibration procedure and recommended occurrence. The pH standards can be purchased through many suppliers. ? ? Jon Henry St. Onge, HT(ASCP) Quality Control Supervisor Dako North America -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Tuesday, February 01, 2011 5:54 AM To: histonet Subject: [Histonet] calibration of pH meter Good morning everyone, I am curious how frequently you all calibrate your pH meter, particularly when you make up solutions for muscle bx's? Do you do it daily, weekly, or monthly? I am trying to come up with an acceptable standard in a clinical histology lab, verses a biotech lab, which makes-up large quantities of reagents on a daily basis. Thank you in advance for your information, Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any attachments transmitted with it are intended for the addressee(s) stated above only and may contain proprietary and confidential information. If you are not the named addressee, you are hereby notified that any unauthorized reading, disclosure, copying or distribution of this e-mail or use of information contained herein is strictly prohibited and may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete it from your system immediately hereafter. Thank you.This e-mail and any attachments transmitted with it are intended for the addressee(s) stated above only and may contain proprietary and confidential information. If you are not the named addressee, you are hereby notified that any unauthorized reading, disclosure, copying or distribution of this e-mail or use of information contained herein is strictly prohibited and may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete it from your system immediately hereafter. Thank you. From trathborne <@t> somerset-healthcare.com Tue Feb 1 10:53:49 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Feb 1 10:55:18 2011 Subject: [Histonet] Immunohistochemistry validation In-Reply-To: <8CD90410AA970AE-1CD8-4B34@webmail-d100.sysops.aol.com> Message-ID: I just received a copy of HISTOLOGIC, December 2010, which is provided by Sakura. The cover article is titled "Antibody Optimization and Validation". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of jcarpenter764@aol.com Sent: Tuesday, February 01, 2011 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunohistochemistry validation Does anyone have any good advice or good articles I can read about validating immuno stains? thanks. Jennell = _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From JWeems <@t> sjha.org Tue Feb 1 10:58:17 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 1 10:58:23 2011 Subject: [Histonet] Immunohistochemistry validation In-Reply-To: References: <8CD90410AA970AE-1CD8-4B34@webmail-d100.sysops.aol.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081DE58CBC@CHEXCMS10.one.ads.che.org> Wonder when it will be on line? I just looked and the most recent seems to be June. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, February 01, 2011 11:54 To: jcarpenter764@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immunohistochemistry validation I just received a copy of HISTOLOGIC, December 2010, which is provided by Sakura. The cover article is titled "Antibody Optimization and Validation". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of jcarpenter764@aol.com Sent: Tuesday, February 01, 2011 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunohistochemistry validation Does anyone have any good advice or good articles I can read about validating immuno stains? thanks. Jennell = _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From MLunetta <@t> luhcares.org Tue Feb 1 11:02:13 2011 From: MLunetta <@t> luhcares.org (Matt Lunetta) Date: Tue Feb 1 11:02:27 2011 Subject: [Histonet] Immunohistochemistry slides drying and baking Message-ID: <4D47DA25020000A8000553F5@ns.luhcares.org> Morning, We have ours in the oven at 65 for a minimum of 30mins. We are also using the DAKO PTlink system. It works great for us Matt Lunetta HT (ASCP) Longmont United Hospital Message: 2 Date: Mon, 31 Jan 2011 06:30:55 -0500 From: "Kuhnla, Melissa" Subject: RE: [Histonet] Immunohistochemistry slides drying and baking protocol To: "Gomez, Milton" , Message-ID: Content-Type: text/plain; charset="us-ascii" Good Mornig, We currently dry slides at room temp for 15 minutes then bake for 2 hours at 60 degrees. Works well. Melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton Sent: Sunday, January 30, 2011 9:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Immunohistochemistry slides drying and baking protocol Hello Histonetters, How and for how long and at what temperature is everyone air drying and baking slides for IHC staining?; specially for breast and cytology slides. Thank you very much in advance, MG ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. 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From foreightl <@t> gmail.com Tue Feb 1 11:07:39 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Feb 1 11:07:44 2011 Subject: [Histonet] Immunohistochemistry validation In-Reply-To: <8CD90410AA970AE-1CD8-4B34@webmail-d100.sysops.aol.com> References: <8CD90410AA970AE-1CD8-4B34@webmail-d100.sysops.aol.com> Message-ID: Jennell, The 2007 article from Applied immunohistochemistry & molecular morphology "Recommendations for Improved Standardization of Immunohistochemistry" by Goldstein et.al. is the article we used to setup our validation protocols. It is a very good idea to get a head start on a thorough validation protocol. While everything in the article isn't required now, it does seem that the field of IHC is headed in that direction. Good luck. On Tue, Feb 1, 2011 at 8:05 AM, wrote: > > Does anyone have any good advice or good articles I can read about validating immuno stains? > ?thanks. Jennell > > > > = > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From algranth <@t> email.arizona.edu Tue Feb 1 11:21:00 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Feb 1 11:21:17 2011 Subject: [Histonet] calibration of pH meter In-Reply-To: References: Message-ID: <9D7E521B-9590-420C-A46C-D96DF8045093@email.arizona.edu> Thanks John for this - I have been looking for such an article. I don't use my pH meter often and so I do calibrate it every time I use it with the pH4.0 and 7.0 buffers. Andi Grantham On Feb 1, 2011, at 9:49 AM, John Kiernan wrote: > As a student I was told to calibrate the pH meter every time it was used (which was about twice a day at the time), using standard buffers at pH4.0 and 7.0. Nearly 50 years later this is still good advice, and modern meters are much less trouble to calibrate than older ones. If you have to make frequent measurements with closely similar solutions, you might get away with calibrating twice a day. > > It's the electrode, not the meter, that changes with time. All pH electrodes deteriorate with time, use and (especially) neglect. A slow response indicates that a new electrode is needed. > > Wikipedia has a nice article about pH meters, and there's a web site http://www.ph-meter.info/pH-electrode-calibration that gives advice about calibrating older and newer instruments. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Akemi Allison > Date: Tuesday, February 1, 2011 8:56 > Subject: [Histonet] calibration of pH meter > To: histonet > >> Good morning everyone, >> >> I am curious how frequently you all calibrate your pH meter, >> particularly when you make up solutions for muscle bx's? >> Do you do it daily, weekly, or monthly? I am trying to >> come up with an acceptable standard in a clinical histology lab, >> verses a biotech lab, which makes-up large quantities of >> reagents on a daily basis. >> >> Thank you in advance for your information, >> Akemi >> >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria <@t> gmail.com Tue Feb 1 11:34:35 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Feb 1 11:34:40 2011 Subject: [Histonet] Infectious waste In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C0C3C49F4@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C0C3C49F4@mailsvr.MARSHMED.local> Message-ID: Hi You may receive many opinions on this --- just a heads up. Many clinical labs do not consider shavings or debris from cutting to be red bag (hazardous) waste as they are no longer a 'biological hazard' due to the processing process which incorporates heat, alcohol and a fixative that is expected to effectively kill any infectious agents. There are some exceptions, which you can find in the CDC or OSHA guidelines such as dealing with tissue of brain (Jakob-creutzfeldt) lung (TB or other air-borne pathogens). CAP also has requirements/guidelines for handling these cases which you will need to have documentation for the processing procedure. I would suggest that you refer to these guidelines and also to your institutions regulations on what is regarded as hazardous waste. Above and beyond anything else make sure you document and site in your procedures manual your sources. Hope this will help you. Vikki PS - any spelling errors I apologize for in advance ;-) On Tue, Feb 1, 2011 at 11:45 AM, Martin, Gary wrote > We are wondering if paraffin block shavings are considered infectious > waste. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gmartin <@t> marshallmedical.org Tue Feb 1 11:40:20 2011 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Feb 1 11:40:31 2011 Subject: [Histonet] Infectious waste In-Reply-To: References: <6ED9D4252F278841A0593D3D788AF24C0C3C49F4@mailsvr.MARSHMED.local> Message-ID: <6ED9D4252F278841A0593D3D788AF24C0C3C4BAB@mailsvr.MARSHMED.local> Vikki Thank you for your response. CAP is exactly why I am asking this question. WE do presently have procedures for the exceptions you mentioned. However, it came up in conversation that shavings may be considered "infectious waste". I have yet to find anything in the CAP information that mention this material. I did note your mention of block disposal, and we have that under control per CAP requirements. It's the grey area of shaving we are questioning. Gary From: Victoria Baker [mailto:bakevictoria@gmail.com] Sent: Tuesday, February 01, 2011 9:35 AM To: Martin, Gary Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Infectious waste Hi You may receive many opinions on this --- just a heads up. Many clinical labs do not consider shavings or debris from cutting to be red bag (hazardous) waste as they are no longer a 'biological hazard' due to the processing process which incorporates heat, alcohol and a fixative that is expected to effectively kill any infectious agents. There are some exceptions, which you can find in the CDC or OSHA guidelines such as dealing with tissue of brain (Jakob-creutzfeldt) lung (TB or other air-borne pathogens). CAP also has requirements/guidelines for handling these cases which you will need to have documentation for the processing procedure. I would suggest that you refer to these guidelines and also to your institutions regulations on what is regarded as hazardous waste. Above and beyond anything else make sure you document and site in your procedures manual your sources. Hope this will help you. Vikki PS - any spelling errors I apologize for in advance ;-) On Tue, Feb 1, 2011 at 11:45 AM, Martin, Gary wrote We are wondering if paraffin block shavings are considered infectious waste. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Tue Feb 1 11:57:20 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Feb 1 11:57:54 2011 Subject: [Histonet] CAP question ANP.22970 Message-ID: We are having our inspection this spring and I am working to get all our procedures, etc. ready. I am having trouble finding benchmark information for comparison for HER2 to comply with this question - ..."the laboratory at least annually compares its patient results with published benchmarks,".... We are using the Dako Herceptest. I spoke with Dako tech services and they did not have any information. What are other labs using for a benchmark. Thanks in advance for all your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From Jessica.Vacca <@t> HCAhealthcare.com Tue Feb 1 12:19:10 2011 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Tue Feb 1 12:19:16 2011 Subject: [Histonet] intranet P&P Message-ID: <938D716CD445614ABBB817517557B6F4F9A61FC0@NADCWPMSGCMS09.hca.corpad.net> For those of you that are using an electronic system for your policy and procedures- Is anyone strictly using "SharePoint"? (no 3rd party document control software) Do you also keep a paper copy or digital (disk, thumb drive) available? Which would most recommend? How are you reviewing individual procedures annually? How are you documenting this? When you review electronically and you have paper copies are you going to the paper copy and documenting there as well? Trying to move into technology and have a 1-stop shop for our lab. Thanks in advance for your responses. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon, FL 33511 8133571.6410 (office) 813.571.5169 (fax) From shive003 <@t> umn.edu Tue Feb 1 12:36:45 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Feb 1 12:36:48 2011 Subject: [Histonet] calibration of pH meter References: <6D995FF4-53C4-4C83-8A41-73E848C0F3CA@yahoo.com> Message-ID: <69C723DA1B964BAAAA97A6C6454FB55F@auxs.umn.edu> We calibrate our pH meter every day that it's used, which can be daily during heavy workload weeks, using multiple calibration buffers. Jan Shivers UMN Vet Diag Lab ----- Original Message ----- From: "Akemi Allison" To: "histonet" Sent: Tuesday, February 01, 2011 7:54 AM Subject: [Histonet] calibration of pH meter > Good morning everyone, > > I am curious how frequently you all calibrate your pH meter, particularly > when you make up solutions for muscle bx's? Do you do it daily, weekly, > or monthly? I am trying to come up with an acceptable standard in a > clinical histology lab, verses a biotech lab, which makes-up large > quantities of reagents on a daily basis. > > Thank you in advance for your information, > Akemi > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Wanda.Smith <@t> HCAhealthcare.com Tue Feb 1 12:42:16 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Tue Feb 1 12:42:19 2011 Subject: [Histonet] RE: CAP question ANP.22970 In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EACF6D4@NADCWPMSGCMS03.hca.corpad.net> Martha, I spoke to Joan at CAP just yesterday regarding this question. I too, am getting ready for CAP, so I feel your pain!!!!! She said you can use the benchmarks in the notes under the question in the checklist or you can used other published articles that offer benchmark data. Just state what your reference is and where it is from. She also said you can use proficiency testing data. Hope this helps, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Tuesday, February 01, 2011 12:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question ANP.22970 We are having our inspection this spring and I am working to get all our procedures, etc. ready. I am having trouble finding benchmark information for comparison for HER2 to comply with this question - ..."the laboratory at least annually compares its patient results with published benchmarks,".... We are using the Dako Herceptest. I spoke with Dako tech services and they did not have any information. What are other labs using for a benchmark. Thanks in advance for all your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robin_dean <@t> compbio.com Tue Feb 1 13:44:40 2011 From: robin_dean <@t> compbio.com (Robin Dean) Date: Tue Feb 1 13:47:44 2011 Subject: [Histonet] stains for visualizing new bone growth Message-ID: <002601cbc248$77944270$66bcc750$@com> Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com From susanbachus <@t> verizon.net Tue Feb 1 13:49:09 2011 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Tue Feb 1 13:49:21 2011 Subject: [Histonet] calibration of pH meter In-Reply-To: <69C723DA1B964BAAAA97A6C6454FB55F@auxs.umn.edu> References: <6D995FF4-53C4-4C83-8A41-73E848C0F3CA@yahoo.com> <69C723DA1B964BAAAA97A6C6454FB55F@auxs.umn.edu> Message-ID: <34DB990181534DB5A39064EEAF482D08@OwnerPC> This may sound simplistic, but it works for us: whenever I need to use pH something, I quickly first dunk the electrode in the buffers above & below what I'm pHing (rinsing after each dunk, of course)--if the meter reads accurately I proceed to measure, if it's off by more than a few % I recalibrate before measuring. It's true that a new electrode can stay well calibrated for long periods. Susan ----- Original Message ----- From: "Jan Shivers" To: "histonet" ; "Akemi Allison" Sent: Tuesday, February 01, 2011 1:36 PM Subject: Re: [Histonet] calibration of pH meter > We calibrate our pH meter every day that it's used, which can be daily > during heavy workload weeks, using multiple calibration buffers. > > Jan Shivers > UMN Vet Diag Lab > > ----- Original Message ----- > From: "Akemi Allison" > To: "histonet" > Sent: Tuesday, February 01, 2011 7:54 AM > Subject: [Histonet] calibration of pH meter > > >> Good morning everyone, >> >> I am curious how frequently you all calibrate your pH meter, >> particularly when you make up solutions for muscle bx's? Do you do it >> daily, weekly, or monthly? I am trying to come up with an acceptable >> standard in a clinical histology lab, verses a biotech lab, which >> makes-up large quantities of reagents on a daily basis. >> >> Thank you in advance for your information, >> Akemi >> >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Tue Feb 1 14:01:16 2011 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Feb 1 14:01:23 2011 Subject: [Histonet] RE: CAP question ANP.22970 In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EACF6D4@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EACF6D4@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <4D48122C.5D38.00EF.0@swmail.sw.org> The published article we use is "Hormone Receptor Status & Survival in a Population-Based Cohort of Patients with Breast Carcinoma" www.interscience.wiley.com April 20, 2005 Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> 2/1/2011 12:42 PM >>> Martha, I spoke to Joan at CAP just yesterday regarding this question. I too, am getting ready for CAP, so I feel your pain!!!!! She said you can use the benchmarks in the notes under the question in the checklist or you can used other published articles that offer benchmark data. Just state what your reference is and where it is from. She also said you can use proficiency testing data. Hope this helps, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Tuesday, February 01, 2011 12:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question ANP.22970 We are having our inspection this spring and I am working to get all our procedures, etc. ready. I am having trouble finding benchmark information for comparison for HER2 to comply with this question - ..."the laboratory at least annually compares its patient results with published benchmarks,".... We are using the Dako Herceptest. I spoke with Dako tech services and they did not have any information. What are other labs using for a benchmark. Thanks in advance for all your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From shive003 <@t> umn.edu Tue Feb 1 14:13:09 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Feb 1 14:13:13 2011 Subject: [Histonet] calibration of pH meter References: <6D995FF4-53C4-4C83-8A41-73E848C0F3CA@yahoo.com><69C723DA1B964BAAAA97A6C6454FB55F@auxs.umn.edu> <34DB990181534DB5A39064EEAF482D08@OwnerPC> Message-ID: For our equipment quality control, we're required to record every single calibration value each time we calibrate/verify pHs. The document is reviewed regularly to see if there are any abnormal out-of-range pH-ing trends starting to happen. We also aren't allowed to put the probe into the various pH buffer bottles, but need to pour buffers into separate beakers and use them once, then toss. Jan Shivers ----- Original Message ----- From: "Susan Bachus" To: "histonet" ; "Akemi Allison" Sent: Tuesday, February 01, 2011 1:49 PM Subject: Re: [Histonet] calibration of pH meter > This may sound simplistic, but it works for us: whenever I need to use > pH something, I quickly first dunk the electrode in the buffers above & > below what I'm pHing (rinsing after each dunk, of course)--if the meter > reads accurately I proceed to measure, if it's off by more than a few % I > recalibrate before measuring. It's true that a new electrode can stay > well calibrated for long periods. Susan > ----- Original Message ----- > From: "Jan Shivers" > To: "histonet" ; "Akemi Allison" > > Sent: Tuesday, February 01, 2011 1:36 PM > Subject: Re: [Histonet] calibration of pH meter > > >> We calibrate our pH meter every day that it's used, which can be daily >> during heavy workload weeks, using multiple calibration buffers. >> >> Jan Shivers >> UMN Vet Diag Lab >> >> ----- Original Message ----- >> From: "Akemi Allison" >> To: "histonet" >> Sent: Tuesday, February 01, 2011 7:54 AM >> Subject: [Histonet] calibration of pH meter >> >> >>> Good morning everyone, >>> >>> I am curious how frequently you all calibrate your pH meter, >>> particularly when you make up solutions for muscle bx's? Do you do it >>> daily, weekly, or monthly? I am trying to come up with an acceptable >>> standard in a clinical histology lab, verses a biotech lab, which >>> makes-up large quantities of reagents on a daily basis. >>> >>> Thank you in advance for your information, >>> Akemi >>> >>> >>> Akemi Allison BS, HT (ASCP) HTL >>> Director >>> Phoenix Lab Consulting >>> Tele: 408.335.9994 >>> E-Mail: akemiat3377@yahoo.com >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Tue Feb 1 14:16:21 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Feb 1 14:16:24 2011 Subject: [Histonet] calibration of pH meter Message-ID: Hi Akemi, I calibrate every other day that it is in use (Mon, Wed, Fri). I would do it more often if it were required. As it is there is very little drift from day to day. I have noticed more drift if I don't use it one day and it goes to day three. If I'm making something up that I know to be extremely finicky I calibrate it again just for good measure. Honestly calibration should be so routine that it is almost automatic. Amos On Tue, Feb 1, 2011 at 12:22 PM, wrote: > Message: 12 > Date: Tue, 1 Feb 2011 05:54:26 -0800 > From: Akemi Allison > Subject: [Histonet] calibration of pH meter > To: histonet > Message-ID: <6D995FF4-53C4-4C83-8A41-73E848C0F3CA@yahoo.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; > format=flowed > > Good morning everyone, > > I am curious how frequently you all calibrate your pH meter, > particularly when you make up solutions for muscle bx's? Do you do > it daily, weekly, or monthly? I am trying to come up with an > acceptable standard in a clinical histology lab, verses a biotech > lab, which makes-up large quantities of reagents on a daily basis. > > Thank you in advance for your information, > Akemi > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > From liz <@t> premierlab.com Tue Feb 1 14:23:00 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Feb 1 14:23:04 2011 Subject: [Histonet] stains for visualizing new bone growth In-Reply-To: <002601cbc248$77944270$66bcc750$@com> Message-ID: Robin I'm not sure how accurate this is, but we do a lot of massons trichrome staining in rat muscle pouch studies (they inject into the muscle pouch something that will induce bone formation) and we have noticed that new bone formation has a tendency to stain blue rather than red. You could also try a Goldners trichrome too. That is supposed to stain new bone formation. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Dean Sent: Tuesday, February 01, 2011 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stains for visualizing new bone growth Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Tue Feb 1 14:31:52 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Feb 1 14:31:57 2011 Subject: [Histonet] Histo job openings responses Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4763A@nmdamailsvr.nmda.ad.nmsu.edu> I got some vague and wispy figures from several people that responded and I checked the various websites where histo jobs are listed. From the online listings alone, I came up with some 400 - knowing full well that some postings are duplicates AND taking into account that one source mentioned a number she'd heard floating around - I estimated (in my little in-house project) that there were between 250 and 500 histology job openings across the country. This is not a scientific poll and the results don't even warrant a percentage of deviation but I promised I'd post this info. We ought to have the motto - "each one recruit one". All of us out there in histocyberville could adopt some brilliant but unknowing candidate and mold them into our clone. That way, the bosses wouldn't even know we were gone when we retire. How's that??? Seriously, though - thank you for your vague and ballparky estimates. You are my inspiration, blah...blah...blah... Can you tell I'm only a YEAR away from being one of those train-a-cloners? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From alyssa <@t> alliedsearchpartners.com Tue Feb 1 14:33:14 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Feb 1 14:37:24 2011 Subject: [Histonet] Histology Job Open in Fort Myers, FL Message-ID: Allied Search Partners is currently looking for a qualified Histotech to work full time/permanently in Fort Myers, FL. *Position Title: *Histotech III (Lead) *Reports To: *Laboratory Director *Shift: *Monday-Friday, 8am-5pm * * *Location:* * * Dermatology Pathology Laboratory for well established busy office in Fort Myers, FL founded in 2000. * * *Requirements:* * * - ASCP certification required** ** *Summary:* * * - Responsible for 2 other employees within the laboratory** - Work with the Director of Compliance on compliancy of the lab** - Handles all laboratory compliance** - Help with day to day routine histology *Benefits:* Health Insurance, Dental Insurance, Vision Insurance. Group Life, Short Term Disability and Long Term Disability paid by employer. Voluntary Life for self, spouse, and child. Discount on employer products and services. PTO for vacation, personal time, sick etc. Paid Holidays (7): New Years Day, Memorial Day, Fourth of July, Labor Day, Thanksgiving Day, Day after Thanksgiving Day, and Christmas Day. Bereavement Leave.* *Bi-annual bonuses, performance reviews, uniform and name badge. Direct Depost & 401K with employer contribution. *To apply:* * * Please send resume and salary expectations to Alyssa@alliedsearchpartners.com . At that time we will contact you to conduct a phone screen. Thank you! -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From vjp2105 <@t> columbia.edu Tue Feb 1 14:54:51 2011 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Tue Feb 1 14:54:57 2011 Subject: [Histonet] stains for visualizing new bone growth In-Reply-To: <002601cbc248$77944270$66bcc750$@com> Message-ID: You could do a modified tetrachrome stain, this distinguishes newly woven bone. On 2/1/11 2:44 PM, "Robin Dean" wrote: Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From batesf <@t> ohsu.edu Tue Feb 1 15:37:41 2011 From: batesf <@t> ohsu.edu (Florence Leomiti) Date: Tue Feb 1 15:37:51 2011 Subject: [Histonet] calibration of pH meter In-Reply-To: References: Message-ID: <311B5F326A1C0E4D8CACC6F278FCACEA04CD8B9D72@EX-MB07.ohsu.edu> Hi Akemi I calibrate it weekly. And also document each calibration as you know with the muscle panels especially for the ATP's they have to be accurate. Flo Florence Leomiti HT (ASCP) Neuromuscular Lab Tech. Phone 503-494-6781 Fax 503-418-4249 Pager 16822 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Tuesday, February 01, 2011 12:16 PM To: akemiat3377@yahoo.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] calibration of pH meter Hi Akemi, I calibrate every other day that it is in use (Mon, Wed, Fri). I would do it more often if it were required. As it is there is very little drift from day to day. I have noticed more drift if I don't use it one day and it goes to day three. If I'm making something up that I know to be extremely finicky I calibrate it again just for good measure. Honestly calibration should be so routine that it is almost automatic. Amos On Tue, Feb 1, 2011 at 12:22 PM, wrote: > Message: 12 > Date: Tue, 1 Feb 2011 05:54:26 -0800 > From: Akemi Allison > Subject: [Histonet] calibration of pH meter > To: histonet > Message-ID: <6D995FF4-53C4-4C83-8A41-73E848C0F3CA@yahoo.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; > format=flowed > > Good morning everyone, > > I am curious how frequently you all calibrate your pH meter, > particularly when you make up solutions for muscle bx's? Do you do > it daily, weekly, or monthly? I am trying to come up with an > acceptable standard in a clinical histology lab, verses a biotech > lab, which makes-up large quantities of reagents on a daily basis. > > Thank you in advance for your information, > Akemi > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 1 15:48:31 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 1 15:48:35 2011 Subject: [Histonet] stains for visualizing new bone growth In-Reply-To: Message-ID: <778550.97307.qm@web65715.mail.ac4.yahoo.com> You could use May Grunwald-Giemsa modified for tissue (Maximov's procedure). Ren? J. --- On Tue, 2/1/11, Vanessa J. Phelan wrote: From: Vanessa J. Phelan Subject: Re: [Histonet] stains for visualizing new bone growth To: "Robin Dean" , "histonet@lists.utsouthwestern.edu" Date: Tuesday, February 1, 2011, 3:54 PM You could do a modified tetrachrome stain, this distinguishes newly woven bone. On 2/1/11 2:44 PM, "Robin Dean" wrote: Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Tue Feb 1 18:26:35 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Feb 1 18:26:46 2011 Subject: [Histonet] CAP, microwaves and breast fixation times Message-ID: <009101cbc26f$dd533f90$97f9beb0$@imagesbyhopper.com> Hi Histonetters! I have a question regarding the use of microwave tissue processors, the CAP requirement for minimum 6 hours of NBF fixation for breast specimens. Does anyone know if by using the microwave, the need for the 6 hours fixation can be reduced? I want to make sure to follow the CAP requirements, but wondered if there were any exceptions/changes etc with regards to using the microwave? Any help would be appreciated. tia, Michelle From lblazek <@t> digestivespecialists.com Tue Feb 1 18:33:34 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Feb 1 18:33:39 2011 Subject: [Histonet] RE: Histo job openings responses In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4763A@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E4763A@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EB7EE0172@IBMB7Exchange.digestivespecialists.com> Sally, You are one that could never be cloned! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, February 01, 2011 3:32 PM To: Histonet Subject: [Histonet] Histo job openings responses I got some vague and wispy figures from several people that responded and I checked the various websites where histo jobs are listed. From the online listings alone, I came up with some 400 - knowing full well that some postings are duplicates AND taking into account that one source mentioned a number she'd heard floating around - I estimated (in my little in-house project) that there were between 250 and 500 histology job openings across the country. This is not a scientific poll and the results don't even warrant a percentage of deviation but I promised I'd post this info. We ought to have the motto - "each one recruit one". All of us out there in histocyberville could adopt some brilliant but unknowing candidate and mold them into our clone. That way, the bosses wouldn't even know we were gone when we retire. How's that??? Seriously, though - thank you for your vague and ballparky estimates. You are my inspiration, blah...blah...blah... Can you tell I'm only a YEAR away from being one of those train-a-cloners? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ktuttle <@t> umm.edu Tue Feb 1 21:29:25 2011 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Tue Feb 1 21:29:32 2011 Subject: [Histonet] Off Topic: researchers very funny Message-ID: <4D4889450200001A0005B3B9@GWIA2.umm.edu> If this is a repost I apologize :) http://www.youtube.com/watch?v=Fl4L4M8m4d0&feature=player_embedded This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From amitapandey <@t> torrentpharma.com Tue Feb 1 23:03:02 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Tue Feb 1 23:05:56 2011 Subject: [Histonet] Rat endothelial cell immunohistochemistry Message-ID: Good morning to all members, I am new to histonet , would appreciate your opinion on selection of antibody for rat endothelial cell immunohistochemistry staining. I wish to perform IHC on paraffin section of kidney of SD rats (ischemia reperfusion of renal artery model). I am confused with selection for CD-31 or RECA-1, which will give me the better result to visualize the mature or damaged endothelial cells. I have sort listed the santacruz sc-1506 Ab (CD-31) or abcam (RECA-1), but not sure about its result on paraffin section. Which antibody should i select or do you suggest me to use other antibody? Your view will be greatly appreciated. Dr. Amita Dubey Pathologist PCSED, Toprrent Research Centre Village Bhat Gandhinagar Gujarat,India-382428 From jkiernan <@t> uwo.ca Wed Feb 2 00:55:48 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Feb 2 00:55:55 2011 Subject: [Histonet] RE: paraffin sections: white with airbubbles Message-ID: You can't go from 70% alcohol into paraffin without passing through 100% alcohol and then a clearing agent (liquid miscible with 100% alcohol and with melted wax). Xylene is a commonly used clearing agent. Your times in 50% and 70% alcohol are much longer than necessary. Even for a whale's hypothalamus a few hours in each solvent step should be adequate. In Belgium you may be able to obtain a great classic of histotechnology: Gabe, M (1968) Techniques histologiques. Masson et Cie, Paris. 1113 pages! This explains tissue processing very thoroughly. The author was an academic zoologist who did all his own lab work and made sure he knew what he was about. Get a copy if you can. The English translation of Manfred Gabe's book was posthumously published in 1976. I bought one then and learned a lot from it. Like many histotechnology classics, this book is now a prize possession, almost unobtainable on web sites for second-hand books. 'nuff sed. John Kiernan UWO = = = > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of An Eerdekens > Sent: Friday, January 28, 2011 4:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] paraffin sections: white with airbubbles > > > Dear collegues, > > I am experiencing following problem. > > I have embedded hypothalamus tissue in paraffin using the > following procedure: -fixation in 4% paraformaldehyde for 48 > hours, fixation of the tissue in 50% alcohol, next day in 70% > alcohol,next day paraffin embedding. During the paraffin > embedding there was a short circuit and the machine did not work > for any hours, so there was a delay in the process. > > Now I am making slices of 5 micrometer, using the Microm HM 360. > > The tissue is very white (looks like I am making much thicker > sections) on the slices with airbells inside. I don't have an > explanation for this and many samples are showing the same features. > > Does someone know what might be the reason? > > Thanks for the help. > > Regards, > > An Eerdekens > Laboratory of Intensive Care Medicine > Catholic University Leuven, Belgium > > 003216330518 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amitapandey <@t> torrentpharma.com Wed Feb 2 02:56:58 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Wed Feb 2 03:00:03 2011 Subject: [Histonet] RE: paraffin sections: white with airbubbles In-Reply-To: References: Message-ID: Thomas- This happens when dehydration is not proper, it seems you have directly gone from 70% alcohol to paraffin, even sometimes we have faced this kind of problem in our lab even after following the right protocol. My advice is not to spend too much of time on those tissues and if u have another piece of fixed tissue , process it with standard paraffin processing protocol. Dr. Amita Dubey PCSED,TRC From: John Kiernan To: "Thomas, Nancy" Cc: "histonet@lists.utsouthwestern.edu" , 'An Eerdekens' Date: 02/02/11 12:28 PM Subject: Re: [Histonet] RE: paraffin sections: white with airbubbles Sent by: histonet-bounces@lists.utsouthwestern.edu You can't go from 70% alcohol into paraffin without passing through 100% alcohol and then a clearing agent (liquid miscible with 100% alcohol and with melted wax). Xylene is a commonly used clearing agent. Your times in 50% and 70% alcohol are much longer than necessary. Even for a whale's hypothalamus a few hours in each solvent step should be adequate. In Belgium you may be able to obtain a great classic of histotechnology: Gabe, M (1968) Techniques histologiques. Masson et Cie, Paris. 1113 pages! This explains tissue processing very thoroughly. The author was an academic zoologist who did all his own lab work and made sure he knew what he was about. Get a copy if you can. The English translation of Manfred Gabe's book was posthumously published in 1976. I bought one then and learned a lot from it. Like many histotechnology classics, this book is now a prize possession, almost unobtainable on web sites for second-hand books. 'nuff sed. John Kiernan UWO = = = > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of An Eerdekens > Sent: Friday, January 28, 2011 4:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] paraffin sections: white with airbubbles > > > Dear collegues, > > I am experiencing following problem. > > I have embedded hypothalamus tissue in paraffin using the > following procedure: -fixation in 4% paraformaldehyde for 48 > hours, fixation of the tissue in 50% alcohol, next day in 70% > alcohol,next day paraffin embedding. During the paraffin > embedding there was a short circuit and the machine did not work > for any hours, so there was a delay in the process. > > Now I am making slices of 5 micrometer, using the Microm HM 360. > > The tissue is very white (looks like I am making much thicker > sections) on the slices with airbells inside. I don't have an > explanation for this and many samples are showing the same features. > > Does someone know what might be the reason? > > Thanks for the help. > > Regards, > > An Eerdekens > Laboratory of Intensive Care Medicine > Catholic University Leuven, Belgium > > 003216330518 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Wed Feb 2 04:12:33 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Feb 2 04:12:41 2011 Subject: [Histonet] stains for visualizing new bone growth In-Reply-To: <002601cbc248$77944270$66bcc750$@com> References: <002601cbc248$77944270$66bcc750$@com> Message-ID: Robin, I typically stain first with Von Kossa and then counter with MacNeal's. This provides a very nice contrast where obviously mature mineralized bone is black and newly formed bone (osteoid) is grayish-green color. Additionally, your marrow space is nicely contrasted with clear visualization of osteoblasts and osteoclasts lining the bone surface. At the microscope this is a one-stop-shop stain for collecting static bone histomorphometry. Another nice contrasting stain is a modified Goldner's trichrome stain. With this stain cell nuclei are stained first with a Weigert's (iron) hematoxylin, then newly formed bone (osteoid) is stained red with an acid fuchsin/ponceau stain, next an orange G cytoplasmic stain covers the rest and a light green SF yellowish stain follows up with a nice green contrast of the mineralized bone. Very clear differentiation between mineralized bone (green) and newly formed bone (red). This stains works very well with auto threshold functions on some histomorph systems as it has a very nice contrast for the software to recognize. Of course Masson's trichrome works as well but it is typically used on decalcified paraffin embedded sections. I have found the Masson's staining kit at Sigma-Aldrich and kits for all the other stains mentioned can be found at Dorn and Hart Microedge. In fact, Dorn and Hart Microedge (www.dornandhart.com) has a lot to offer now with regards to mineralized bone (hard tissue with or without implant materials) and resin embedded histology. Good luck to you and let me know if you have any additional questions. I would also be happy to share images with you if interested. Jack On Feb 1, 2011, at 1:44 PM, "Robin Dean" wrote: > Does anyone know of a good stain to use to clearly show new bone growth > other than von Kossa stain? > > Would appreciate any suggestions anyone might have. > > > > Thank you, > > > > Robin > > Robin R. Dean, Ph.D. > > Senior Scientist & Study Director > > Comparative Biosciences, Inc. > > 786 Lucerne Dr. > > Sunnyvale, CA > > (408) 738-8060 > > robin_dean@compbio.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Melissa.Kuhnla <@t> chsli.org Wed Feb 2 05:26:12 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Feb 2 05:26:18 2011 Subject: [Histonet] calibration of pH meter In-Reply-To: References: <6D995FF4-53C4-4C83-8A41-73E848C0F3CA@yahoo.com><69C723DA1B964BAAAA97A6C6454FB55F@auxs.umn.edu><34DB990181534DB5A39064EEAF482D08@OwnerPC> Message-ID: Is anyone aware of any actual standards? I routinely calibrate the ph meter each time we use it. I calibrate, and then take the reading of the newly made buffer. Is this sufficient? I recently had my compliance officer tell me the this was not sufficient. She thinks we need to calibrate, take a reading of a solution with a known ph, and then take the reading of the new solution. What is everyone doing??????? Thank you, Melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 01, 2011 3:13 PM To: Susan Bachus; histonet; Akemi Allison Subject: Re: [Histonet] calibration of pH meter For our equipment quality control, we're required to record every single calibration value each time we calibrate/verify pHs. The document is reviewed regularly to see if there are any abnormal out-of-range pH-ing trends starting to happen. We also aren't allowed to put the probe into the various pH buffer bottles, but need to pour buffers into separate beakers and use them once, then toss. Jan Shivers ----- Original Message ----- From: "Susan Bachus" To: "histonet" ; "Akemi Allison" Sent: Tuesday, February 01, 2011 1:49 PM Subject: Re: [Histonet] calibration of pH meter > This may sound simplistic, but it works for us: whenever I need to use > pH something, I quickly first dunk the electrode in the buffers above & > below what I'm pHing (rinsing after each dunk, of course)--if the meter > reads accurately I proceed to measure, if it's off by more than a few % I > recalibrate before measuring. It's true that a new electrode can stay > well calibrated for long periods. Susan > ----- Original Message ----- > From: "Jan Shivers" > To: "histonet" ; "Akemi Allison" > > Sent: Tuesday, February 01, 2011 1:36 PM > Subject: Re: [Histonet] calibration of pH meter > > >> We calibrate our pH meter every day that it's used, which can be daily >> during heavy workload weeks, using multiple calibration buffers. >> >> Jan Shivers >> UMN Vet Diag Lab >> >> ----- Original Message ----- >> From: "Akemi Allison" >> To: "histonet" >> Sent: Tuesday, February 01, 2011 7:54 AM >> Subject: [Histonet] calibration of pH meter >> >> >>> Good morning everyone, >>> >>> I am curious how frequently you all calibrate your pH meter, >>> particularly when you make up solutions for muscle bx's? Do you do it >>> daily, weekly, or monthly? I am trying to come up with an acceptable >>> standard in a clinical histology lab, verses a biotech lab, which >>> makes-up large quantities of reagents on a daily basis. >>> >>> Thank you in advance for your information, >>> Akemi >>> >>> >>> Akemi Allison BS, HT (ASCP) HTL >>> Director >>> Phoenix Lab Consulting >>> Tele: 408.335.9994 >>> E-Mail: akemiat3377@yahoo.com >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From contact <@t> excaliburpathology.com Wed Feb 2 07:49:57 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Feb 2 07:50:07 2011 Subject: [Histonet] Off Topic: researchers very funny In-Reply-To: <4D4889450200001A0005B3B9@GWIA2.umm.edu> References: <4D4889450200001A0005B3B9@GWIA2.umm.edu> Message-ID: <217328.82615.qm@web1112.biz.mail.sk1.yahoo.com> This is hilarious. I got it on facebook yesterday and sent it to everyone. ? Paula K. Pierce, AAS, BA, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Kimberly Tuttle To: histonet@lists.utsouthwestern.edu Sent: Tue, February 1, 2011 9:29:25 PM Subject: [Histonet] Off Topic: researchers very funny If this is a repost I apologize :) http://www.youtube.com/watch?v=Fl4L4M8m4d0&feature=player_embedded This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jacquitta <@t> earthlink.net Wed Feb 2 09:13:40 2011 From: jacquitta <@t> earthlink.net (Jacquitta Taylor) Date: Wed Feb 2 09:13:44 2011 Subject: [Histonet] Eosin stain for determining new bone old bone Message-ID: <4C4B1F98-D6EF-45DF-A010-851CFFE11696@earthlink.net> Robin this is the protocol we use to showing new bone old bone. Procedure for Eosin Y 0.6 % For Staining Bone Specimen Chemicals EosinY, Sigma E-4382, 100% Ethanol, Phloxine B (Sigma P-4030) Orange G(sodium salt-Sigma O-1625) Preparation: Stock 0.6% Eosin Add 6g of eosin to 900ml 100% Ethanol and100 ml of H20; stir to dissolve. Add 50 ml glacial acetic acid adjust PH to 4.6 and 5.0. The color of the solution will change from opaque green to clear red. Stock 1% Phloxine B Solution: Dissolve 1g of Phloxine B in 100 ml distilled water and filter to make a 1% solution. Stock 2% Orange G Solution: Dissolve 2 g of Orange G in 100ml distilled water and filter to make a 2% solution. Working Solution Eosin 0.6% Make a working solution by adding 6 ml of 1% Phloxine and 6 ml of 2% Orange G to 238 ml of 0.6% eosin. Old bone dark orange. New bone light orange. Stain 15 to 30 seconds depending on what intensity you prefer. From jclark <@t> pcnm.com Wed Feb 2 10:18:23 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Feb 2 10:18:27 2011 Subject: [Histonet] TTF-1 Antibody Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01486A3D@mail.pcnm.com> Good Morning, we are using TTF-1 antibody from Cell Marque (clone 8G7G3/1 mouse monoclonal) on a DAKO autostainer with an LSAB+ platform. We do heat retrieval in the DAKO pascal pressure cooker using Cell Marques Trilogy retrieval solution. We do a lower temp for a longer period of time during the retrieval. I incubate the primary antibody for 1 hour and I still have problems getting the marker to work with any consistency. Where do the rest of you doing this marker get your antibody from and do you have problems getting it to work consistently? Any and all feedback would be appreciated, I'm getting really frustrated. Thanks Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico From BSullivan <@t> shorememorial.org Wed Feb 2 10:31:51 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Wed Feb 2 10:35:18 2011 Subject: [Histonet] TTF-1 Antibody In-Reply-To: <0CDA5E1E01301F4880A8A7A8BCBDA39C01486A3D@mail.pcnm.com> Message-ID: We purchase out TTF-1 antibody from Ventana Medical and use it on their Benchmark XT stainer. I do believe they might purchase this from Cell Marque but I could be wrong. Anyway, we have no problem with this stain. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Joanne Clark" Sent by: To histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] TTF-1 Antibody 02/02/2011 11:18 AM Good Morning, we are using TTF-1 antibody from Cell Marque (clone 8G7G3/1 mouse monoclonal) on a DAKO autostainer with an LSAB+ platform. We do heat retrieval in the DAKO pascal pressure cooker using Cell Marques Trilogy retrieval solution. We do a lower temp for a longer period of time during the retrieval. I incubate the primary antibody for 1 hour and I still have problems getting the marker to work with any consistency. Where do the rest of you doing this marker get your antibody from and do you have problems getting it to work consistently? Any and all feedback would be appreciated, I'm getting really frustrated. Thanks Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Wed Feb 2 11:11:10 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed Feb 2 11:08:49 2011 Subject: [Histonet] Her2 Fixation Requirement Message-ID: <2FB3F34C3F1047489B7F2E0A9E3E96E9@biopath.local> Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA From BSullivan <@t> shorememorial.org Wed Feb 2 11:27:54 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Wed Feb 2 11:31:16 2011 Subject: [Histonet] Her2 Fixation Requirement In-Reply-To: <2FB3F34C3F1047489B7F2E0A9E3E96E9@biopath.local> Message-ID: We receive our lumpectomies and total breasts fresh. They are immediately put in 10 % formalin if no frozen is required and that time is noted on our requisition. Our processing time for formalin and the fixation time is made part of the final report. If we receive a breast biopsy specimen we have asked that they write the time on the requistion that the specimen was placed in 10 % formalin. We set up a procedure that states this. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Paula Lucas" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Her2 Fixation Requirement 02/02/2011 12:11 PM Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Feb 2 11:58:36 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Feb 2 11:58:41 2011 Subject: [Histonet] Her2 Fixation Requirement In-Reply-To: <2FB3F34C3F1047489B7F2E0A9E3E96E9@biopath.local> References: <2FB3F34C3F1047489B7F2E0A9E3E96E9@biopath.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081DE58F2F@CHEXCMS10.one.ads.che.org> We have the nurse document the time of removal on the requisition. The next requisition update will have a spot for this. They have been very cooperative and do a good job. In addition, you must document the "cold ischmic time" - that is the time from removal until time in formalin. This is important when the specimen goes for xray or whatever. So there is a removal time, an into formalin time and an out of formalin time. Then if we don't have time allowed to meet the fixation time with the regular, it is put on a late processor, if we have one available, or it is held overnight. And if it has to come off on Sunday, we have a med tech remove it from the processor and it waits for us to come on Monday to embed it. The pathologists document all this time in the report. Best! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, February 02, 2011 12:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2 Fixation Requirement Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From MLunetta <@t> luhcares.org Wed Feb 2 12:20:12 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Feb 2 12:20:31 2011 Subject: [Histonet] TTF-1 Antibody Message-ID: <4D493DEC020000A8000555AE@ns.luhcares.org> We used the DAKO TTF-1 and have no problems with it. Matt Lunetta HT (ASCP) Longmont United Hopsital Colorado Message: 3 Date: Wed, 2 Feb 2011 09:18:23 -0700 From: "Joanne Clark" Subject: [Histonet] TTF-1 Antibody To: Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01486A3D@mail.pcnm.com> Content-Type: text/plain; charset="us-ascii" Good Morning, we are using TTF-1 antibody from Cell Marque (clone 8G7G3/1 mouse monoclonal) on a DAKO autostainer with an LSAB+ platform. We do heat retrieval in the DAKO pascal pressure cooker using Cell Marques Trilogy retrieval solution. We do a lower temp for a longer period of time during the retrieval. I incubate the primary antibody for 1 hour and I still have problems getting the marker to work with any consistency. Where do the rest of you doing this marker get your antibody from and do you have problems getting it to work consistently? Any and all feedback would be appreciated, I'm getting really frustrated. Thanks Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 4 Date: Wed, 2 Feb 2011 11:31:51 -0500 From: BSullivan@shorememorial.org Subject: Re: [Histonet] TTF-1 Antibody To: "Joanne Clark" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII We purchase out TTF-1 antibody from Ventana Medical and use it on their Benchmark XT stainer. I do believe they might purchase this from Cell Marque but I could be wrong. Anyway, we have no problem with this stain. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Joanne Clark" Sent by: To histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] TTF-1 Antibody 02/02/2011 11:18 AM Good Morning, we are using TTF-1 antibody from Cell Marque (clone 8G7G3/1 mouse monoclonal) on a DAKO autostainer with an LSAB+ platform. We do heat retrieval in the DAKO pascal pressure cooker using Cell Marques Trilogy retrieval solution. We do a lower temp for a longer period of time during the retrieval. I incubate the primary antibody for 1 hour and I still have problems getting the marker to work with any consistency. Where do the rest of you doing this marker get your antibody from and do you have problems getting it to work consistently? Any and all feedback would be appreciated, I'm getting really frustrated. Thanks Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shu-cheng.chen <@t> merck.com Wed Feb 2 12:28:47 2011 From: shu-cheng.chen <@t> merck.com (Chen, Shu-Cheng) Date: Wed Feb 2 12:29:42 2011 Subject: [Histonet] Rat lung histology for mast cells and eosinophils Message-ID: <5D62649615FAA6478F801A08D10E51855983C4A9AF@USCTMXP51003.merck.com> Hi, We are called to support an asthmatic rat lung project and need to stain for mast cells, eosinophils, neutrophils and possibly other inflammatory cells, such as Th2 cells etc. Any suggestions you can give us in terms of fixatives and special stains or IHC are very much appreciated. From my search, it seems that Carnoy's fixative with toluidine blue is the way to go for mast cells. But can one clearly differentiate eos from neuts with this fixative or a formalin fixed H&E stained lung section? If we need to do Trichrome, PAS, May Grunwald Giemsa and H&E etc. what would be the best fixative to accommodate these stains? BTW, I heard that formalin free Zn-fixative is good for IHC. Will it be good for all these stains above? Sorry for so many questions. It shows how little experience I have in this area. Thank you, Shu Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From jcox90 <@t> yahoo.com Wed Feb 2 12:45:50 2011 From: jcox90 <@t> yahoo.com (jcox90@yahoo.com) Date: Wed Feb 2 12:45:53 2011 Subject: [Histonet] Looking for Part time work in Phoenix AZ Message-ID: <322028.69517.qm@web161616.mail.bf1.yahoo.com> Hi all, I am looking for part time work in the Phoenix area, I am HT ascp and have 18 years experience. Please respond to this email, thank you. Jill From liz <@t> premierlab.com Wed Feb 2 12:52:56 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Feb 2 12:53:02 2011 Subject: [Histonet] Rat lung histology for mast cells and eosinophils In-Reply-To: <5D62649615FAA6478F801A08D10E51855983C4A9AF@USCTMXP51003.merck.com> Message-ID: Chen For mast cells you can fix in 10% NBF and be fine with toluidine blue. We stain with toluidine blue on formalin fixed samples all of the time. The trick is not to dehydrate the sections, let them air dry and then mount. I would stick with formalin fixation that's going to be your best bet for most specials and IHC stains. For eosinophils you can stain with giemsa, or we would run a modified dif-quik stain that worked well for eosinophils. For neutrophils I'm not sure but if you are staining for mouse neutrophils, serotec has a nice antibody. Macrophages would be IHC for ED-1 also from serotec, CD3 from Dako will work on rat tissue nicely. There are other markers that will work on cell lines. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chen, Shu-Cheng Sent: Wednesday, February 02, 2011 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat lung histology for mast cells and eosinophils Hi, We are called to support an asthmatic rat lung project and need to stain for mast cells, eosinophils, neutrophils and possibly other inflammatory cells, such as Th2 cells etc. Any suggestions you can give us in terms of fixatives and special stains or IHC are very much appreciated. From my search, it seems that Carnoy's fixative with toluidine blue is the way to go for mast cells. But can one clearly differentiate eos from neuts with this fixative or a formalin fixed H&E stained lung section? If we need to do Trichrome, PAS, May Grunwald Giemsa and H&E etc. what would be the best fixative to accommodate these stains? BTW, I heard that formalin free Zn-fixative is good for IHC. Will it be good for all these stains above? Sorry for so many questions. It shows how little experience I have in this area. Thank you, Shu Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jconnelly <@t> sleh.com Wed Feb 2 13:00:16 2011 From: jconnelly <@t> sleh.com (Connelly, John) Date: Wed Feb 2 13:00:32 2011 Subject: [Histonet] re Her2 neu requirements Message-ID: <0DD7BD0E1F64864A8982BE7EC71492BF0500E96A@NTMS9.sleh.com> We have the specimens sent fresh to our lab and we put them in formalin there. We document the time and the we make sure all are processed in the time window. The diffculty is usually on Friday biopsy and excision specimens, especially if you have a courier run. John Connelly, M.D. "Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. " +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From Lori.Disher <@t> HCAhealthcare.com Wed Feb 2 13:15:32 2011 From: Lori.Disher <@t> HCAhealthcare.com (Lori.Disher@HCAhealthcare.com) Date: Wed Feb 2 13:15:36 2011 Subject: [Histonet] Her2 Fixation Requirement Message-ID: <778DD853CF606049A37FC2059C8BA07A7553FF79D8@FWDCWPMSGCMS04.hca.corpad.net> What is the appropriate time it should be in formalin? Do you start your time when it is initially put into formalin, or the start from the time it was dissected? Lori A Disher Fawcett Memorial Hospital Port Charlotte, FL 33952 lori.disher@hcahealthcare.com From gmartin <@t> marshallmedical.org Wed Feb 2 13:16:20 2011 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Feb 2 13:16:26 2011 Subject: [Histonet] RE: Histonet Digest, Vol 87, Issue 4 In-Reply-To: References: Message-ID: <6ED9D4252F278841A0593D3D788AF24C0C41FB34@mailsvr.MARSHMED.local> We too are a small lab in California, and faced these same questions. We simply document all this in the report. For example; we know that our tissue comes out of formalin on the processor at 2330. We have the surgery staff document the time the specimen went into formalin. We then subtract that time form 2330 and have the hours in formalin. You are correct that it took a great deal of effort to see that surgery complied, but after they realized we were relentless with phone calls, things fell into place. Oh yes! On the weekend someone must come in to remove the tissue from the processor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, February 02, 2011 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Off Topic: researchers very funny (Paula Pierce) 2. Eosin stain for determining new bone old bone (Jacquitta Taylor) 3. TTF-1 Antibody (Joanne Clark) 4. Re: TTF-1 Antibody (BSullivan@shorememorial.org) 5. Her2 Fixation Requirement (Paula Lucas) 6. Re: Her2 Fixation Requirement (BSullivan@shorememorial.org) 7. RE: Her2 Fixation Requirement (Weems, Joyce) ---------------------------------------------------------------------- Message: 1 Date: Wed, 2 Feb 2011 05:49:57 -0800 (PST) From: Paula Pierce Subject: Re: [Histonet] Off Topic: researchers very funny To: Kimberly Tuttle , Histonet Message-ID: <217328.82615.qm@web1112.biz.mail.sk1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This is hilarious. I got it on facebook yesterday and sent it to everyone. ? Paula K. Pierce, AAS, BA, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Kimberly Tuttle To: histonet@lists.utsouthwestern.edu Sent: Tue, February 1, 2011 9:29:25 PM Subject: [Histonet] Off Topic: researchers very funny If this is a repost I apologize :) http://www.youtube.com/watch?v=Fl4L4M8m4d0&feature=player_embedded This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 2 Feb 2011 09:13:40 -0600 From: Jacquitta Taylor Subject: [Histonet] Eosin stain for determining new bone old bone To: histonet@lists.utsouthwestern.edu Message-ID: <4C4B1F98-D6EF-45DF-A010-851CFFE11696@earthlink.net> Content-Type: text/plain; charset=us-ascii Robin this is the protocol we use to showing new bone old bone. Procedure for Eosin Y 0.6 % For Staining Bone Specimen Chemicals EosinY, Sigma E-4382, 100% Ethanol, Phloxine B (Sigma P-4030) Orange G(sodium salt-Sigma O-1625) Preparation: Stock 0.6% Eosin Add 6g of eosin to 900ml 100% Ethanol and100 ml of H20; stir to dissolve. Add 50 ml glacial acetic acid adjust PH to 4.6 and 5.0. The color of the solution will change from opaque green to clear red. Stock 1% Phloxine B Solution: Dissolve 1g of Phloxine B in 100 ml distilled water and filter to make a 1% solution. Stock 2% Orange G Solution: Dissolve 2 g of Orange G in 100ml distilled water and filter to make a 2% solution. Working Solution Eosin 0.6% Make a working solution by adding 6 ml of 1% Phloxine and 6 ml of 2% Orange G to 238 ml of 0.6% eosin. Old bone dark orange. New bone light orange. Stain 15 to 30 seconds depending on what intensity you prefer. ------------------------------ Message: 3 Date: Wed, 2 Feb 2011 09:18:23 -0700 From: "Joanne Clark" Subject: [Histonet] TTF-1 Antibody To: Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01486A3D@mail.pcnm.com> Content-Type: text/plain; charset="us-ascii" Good Morning, we are using TTF-1 antibody from Cell Marque (clone 8G7G3/1 mouse monoclonal) on a DAKO autostainer with an LSAB+ platform. We do heat retrieval in the DAKO pascal pressure cooker using Cell Marques Trilogy retrieval solution. We do a lower temp for a longer period of time during the retrieval. I incubate the primary antibody for 1 hour and I still have problems getting the marker to work with any consistency. Where do the rest of you doing this marker get your antibody from and do you have problems getting it to work consistently? Any and all feedback would be appreciated, I'm getting really frustrated. Thanks Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 4 Date: Wed, 2 Feb 2011 11:31:51 -0500 From: BSullivan@shorememorial.org Subject: Re: [Histonet] TTF-1 Antibody To: "Joanne Clark" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII We purchase out TTF-1 antibody from Ventana Medical and use it on their Benchmark XT stainer. I do believe they might purchase this from Cell Marque but I could be wrong. Anyway, we have no problem with this stain. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Joanne Clark" Sent by: To histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] TTF-1 Antibody 02/02/2011 11:18 AM Good Morning, we are using TTF-1 antibody from Cell Marque (clone 8G7G3/1 mouse monoclonal) on a DAKO autostainer with an LSAB+ platform. We do heat retrieval in the DAKO pascal pressure cooker using Cell Marques Trilogy retrieval solution. We do a lower temp for a longer period of time during the retrieval. I incubate the primary antibody for 1 hour and I still have problems getting the marker to work with any consistency. Where do the rest of you doing this marker get your antibody from and do you have problems getting it to work consistently? Any and all feedback would be appreciated, I'm getting really frustrated. Thanks Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 2 Feb 2011 09:11:10 -0800 From: "Paula Lucas" Subject: [Histonet] Her2 Fixation Requirement To: Message-ID: <2FB3F34C3F1047489B7F2E0A9E3E96E9@biopath.local> Content-Type: text/plain; charset="us-ascii" Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ------------------------------ Message: 6 Date: Wed, 2 Feb 2011 12:27:54 -0500 From: BSullivan@shorememorial.org Subject: Re: [Histonet] Her2 Fixation Requirement To: "Paula Lucas" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII We receive our lumpectomies and total breasts fresh. They are immediately put in 10 % formalin if no frozen is required and that time is noted on our requisition. Our processing time for formalin and the fixation time is made part of the final report. If we receive a breast biopsy specimen we have asked that they write the time on the requistion that the specimen was placed in 10 % formalin. We set up a procedure that states this. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Paula Lucas" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Her2 Fixation Requirement 02/02/2011 12:11 PM Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 2 Feb 2011 12:58:36 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Her2 Fixation Requirement To: Paula Lucas , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081DE58F2F@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We have the nurse document the time of removal on the requisition. The next requisition update will have a spot for this. They have been very cooperative and do a good job. In addition, you must document the "cold ischmic time" - that is the time from removal until time in formalin. This is important when the specimen goes for xray or whatever. So there is a removal time, an into formalin time and an out of formalin time. Then if we don't have time allowed to meet the fixation time with the regular, it is put on a late processor, if we have one available, or it is held overnight. And if it has to come off on Sunday, we have a med tech remove it from the processor and it waits for us to come on Monday to embed it. The pathologists document all this time in the report. Best! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, February 02, 2011 12:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2 Fixation Requirement Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 87, Issue 4 *************************************** From liz <@t> premierlab.com Wed Feb 2 13:27:57 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Feb 2 13:28:01 2011 Subject: [Histonet] Her2 Fixation Requirement In-Reply-To: <778DD853CF606049A37FC2059C8BA07A7553FF79D8@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: Lori I would think both if you could since the Her2 paper does address those issues as potentials for problems in testing variation (time to fixation). I believe the CAP/ASCO paper states not less then 6 and no more than 48 hours for fixation (2007 paper). I believe you also should document type of fixative if possible; vendor, lot number and expiration date if you can. I have pdfs of these documents if you need them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori.Disher@HCAhealthcare.com Sent: Wednesday, February 02, 2011 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement What is the appropriate time it should be in formalin? Do you start your time when it is initially put into formalin, or the start from the time it was dissected? Lori A Disher Fawcett Memorial Hospital Port Charlotte, FL 33952 lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Feb 2 13:44:15 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Feb 2 13:44:19 2011 Subject: [Histonet] Her2 Fixation Requirement In-Reply-To: <778DD853CF606049A37FC2059C8BA07A7553FF79D8@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A7553FF79D8@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081DE58F9F@CHEXCMS10.one.ads.che.org> Formalin time is no less than 6 and no more than 48 hours. You need to document time from excision to time in formalin - cold ischemic time - and time in formalin. New regs extended fixation time for ER/PR to 72 hours, but since it's all in there together, you can't go by that yet. And for patient's to be eligible for many clinical trials, 10% NBF is the only fixative that should be used. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori.Disher@HCAhealthcare.com Sent: Wednesday, February 02, 2011 14:16 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement What is the appropriate time it should be in formalin? Do you start your time when it is initially put into formalin, or the start from the time it was dissected? Lori A Disher Fawcett Memorial Hospital Port Charlotte, FL 33952 lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Luis.Chiriboga <@t> nyumc.org Wed Feb 2 14:07:48 2011 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Wed Feb 2 14:07:51 2011 Subject: [Histonet] Her2 Fixation Requirement In-Reply-To: References: <778DD853CF606049A37FC2059C8BA07A7553FF79D8@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: I believe the two most recent articles from ASCO/CAP on this subject are Hammond, M.E., D.F. Hayes, M. Dowsett, D.C. Allred, K.L. Hagerty, S. Badve, P.L. Fitzgibbons, G. Francis, et al. 2010. American Society of Clinical Oncology/College Of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer. J Clin Oncol 28:2784-2795. Wolff, A.C., M.E. Hammond, J.N. Schwartz, K.L. Hagerty, D.C. Allred, R.J. Cote, M. Dowsett, P.L. Fitzgibbons, et al. 2007. American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. J Clin Oncol 25:118-145. If you do not have access to the literature you can request reprints from the authors or it may be available on the ASCO website? Luis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, February 02, 2011 2:28 PM To: Lori.Disher@HCAhealthcare.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement Lori I would think both if you could since the Her2 paper does address those issues as potentials for problems in testing variation (time to fixation). I believe the CAP/ASCO paper states not less then 6 and no more than 48 hours for fixation (2007 paper). I believe you also should document type of fixative if possible; vendor, lot number and expiration date if you can. I have pdfs of these documents if you need them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori.Disher@HCAhealthcare.com Sent: Wednesday, February 02, 2011 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement What is the appropriate time it should be in formalin? Do you start your time when it is initially put into formalin, or the start from the time it was dissected? Lori A Disher Fawcett Memorial Hospital Port Charlotte, FL 33952 lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From JWeems <@t> sjha.org Wed Feb 2 14:18:50 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Feb 2 14:18:53 2011 Subject: [Histonet] WindoPath Users Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081DE58FCC@CHEXCMS10.one.ads.che.org> How many folks are using this LIS for AP? Any feedback would be appreciated. Thanks! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Kim.Donadio <@t> bhcpns.org Wed Feb 2 14:25:23 2011 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Feb 2 14:25:29 2011 Subject: [Histonet] Trace Gas companies Message-ID: Hi Histonetters Can you all share with me where you get and send in your trace gas analysis badges? Thanks a bunch! :-D Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From cls71877 <@t> sbcglobal.net Wed Feb 2 14:30:11 2011 From: cls71877 <@t> sbcglobal.net (=?utf-8?B?Y2xzNzE4NzdAc2JjZ2xvYmFsLm5ldA==?=) Date: Wed Feb 2 14:30:05 2011 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gV2luZG9QYXRoIFVzZXJz?= Message-ID: <871555.10343.bm@smtp104.mail.ac4.yahoo.com> I have been using WindoPath for a year and a half now and I love it! We are a GI lab so building our library was not as time consuming as a it would be for a full spectrum path lab, but you only have to do that once. We have had four pathologists trained on using the program and they all find it very user friendly as well. Feel free to contact me should you like more information. Thanks, Cristi Sent from my HTC on the Now Network from Sprint! ----- Reply message ----- From: "Weems, Joyce" Date: Wed, Feb 2, 2011 12:18 pm Subject: [Histonet] WindoPath Users To: "histonet@lists.utsouthwestern.edu" How many folks are using this LIS for AP? Any feedback would be appreciated. Thanks! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Herrick.James <@t> mayo.edu Wed Feb 2 16:18:22 2011 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Wed Feb 2 16:19:09 2011 Subject: [Histonet] Safranin O Message-ID: <7267A64D75F58241B577876D8A885631038FE474@msgebe41> Hello everyone, I am trying to stain for cartilage in mouse skull (cross-sectioning with the brain in tact) and wanted to know if anyone might have a good protocol for Safranin O / Fast green or Safranin O / von Kossa? The tissue is embedded in MMA and was cut on a saw microtome and finished down to an approximate 20 to 30 micron tissue thickness - 50 microns including the glue layer (I glue a slide to the block before making the cut and then grind and polish to finish). I tried Weigert's hematoxylin / Safranin O / Fast Green ( I began with Weigert's Hematoxylin, followed by Fast Green and finished with Safranin O), but the Safranin O seemed to overpower the Fast Green (the calcified bone stained more red than green). Any help on this issue would be greatly appreciated as always. Thanks again. Jim From liz <@t> premierlab.com Wed Feb 2 16:47:03 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Feb 2 16:47:17 2011 Subject: [Histonet] EGF IHC Message-ID: Hello everyone Does anyone out there have a good EGF (not EGFR) antibody that works well in formalin fixed paraffin embedded samples - species is human Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From liz <@t> premierlab.com Wed Feb 2 16:55:40 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Feb 2 16:56:08 2011 Subject: [Histonet] slide labeler advice Message-ID: Hello again We are in the market for a slide labeler. We currently have the SlideMate from Thermo Scientific. We have had it for about 2 years now and we are just not happy with the quality and consistency of the printing. We are a contract research lab and not a clinical lab. Entering data for each slide is not an option. The programming that comes with the Slide Mate is nice, except that no one has really spent the time to train us on it, we have figured it out a bit. We would want a labeler that we could create formats for different sample types, so all we would have to do is put the animal ID in and the printer would print all of the slides needed. Would like barcoding capabilities just incase we move to that in the future. Any advice or suggestions are appreciated. Vendors welcome also. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From STACEY.LANGENBERG <@t> UCDENVER.EDU Wed Feb 2 18:36:13 2011 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Wed Feb 2 18:36:30 2011 Subject: [Histonet] slide labeler advice Message-ID: <267D0C09-8776-4E94-9815-35F10DCA2A0B@ucdenver.edu> Hi Liz We love our IPS and IPC printers from leica. Both strong work horses that are adaptable to your needs. Stacey Cu Dermpath Sent from myTouch 4G ----- Reply message ----- From: "Liz Chlipala" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] slide labeler advice Date: Wed, Feb 2, 2011 3:58 pm Hello again We are in the market for a slide labeler. We currently have the SlideMate from Thermo Scientific. We have had it for about 2 years now and we are just not happy with the quality and consistency of the printing. We are a contract research lab and not a clinical lab. Entering data for each slide is not an option. The programming that comes with the Slide Mate is nice, except that no one has really spent the time to train us on it, we have figured it out a bit. We would want a labeler that we could create formats for different sample types, so all we would have to do is put the animal ID in and the printer would print all of the slides needed. Would like barcoding capabilities just incase we move to that in the future. Any advice or suggestions are appreciated. Vendors welcome also. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> medsurgpath.com Wed Feb 2 19:53:30 2011 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Wed Feb 2 19:53:36 2011 Subject: [Histonet] CSF for cytology Message-ID: <269c9413cd1d2f8f650037eeeb9e1c58.squirrel@webmail.integra.net> Hi Histonet, We are going to be receiving some cerebrospinal fluid as party of an MS Panel for cytology. Our pathologist would like me to make a cell button and stain it with H&E and Diff Quik, but I wanted to check with you guys and see if there are any other stains that I might do with this case. Thank you for your help, Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 From AnthonyH <@t> chw.edu.au Wed Feb 2 20:06:38 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Feb 2 20:06:47 2011 Subject: [Histonet] CSF for cytology In-Reply-To: <269c9413cd1d2f8f650037eeeb9e1c58.squirrel@webmail.integra.net> References: <269c9413cd1d2f8f650037eeeb9e1c58.squirrel@webmail.integra.net> Message-ID: <6D6BD1DE8A5571489398B392A38A715706170A@xmdb02.nch.kids> Katelin, CSF specimens usually contain very few cells, maybe a lymphocyte or two. Making a cell button (?cell block for Histo processing) would give disappointing results. I would recommend cytocentrifugation (at least one air-dried DQ stained and one 95% ethanol-fixed PAP stained). You could even concentrate the cells using centrifugation prior to cytocentrifugation. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katelin Lester Sent: Thursday, 3 February 2011 12:54 PM To: histonet Subject: [Histonet] CSF for cytology Hi Histonet, We are going to be receiving some cerebrospinal fluid as party of an MS Panel for cytology. Our pathologist would like me to make a cell button and stain it with H&E and Diff Quik, but I wanted to check with you guys and see if there are any other stains that I might do with this case. Thank you for your help, Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From histology <@t> medsurgpath.com Wed Feb 2 20:11:15 2011 From: histology <@t> medsurgpath.com (Katelin Lester) Date: Wed Feb 2 20:11:21 2011 Subject: [Histonet] Trace Gas companies In-Reply-To: References: Message-ID: <5f8d8d4e5cc7f642003d5b8ffbf96552.squirrel@webmail.integra.net> We just did ours this week! We get them from Environmental Monitoring Technology. http://www.emt-online.com/ Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > Hi Histonetters > > Can you all share with me where you get and send in your trace gas > analysis badges? > > Thanks a bunch! > > :-D > > > > > > > > Kim Donadio > Pathology Supervisor > Baptist Hospital > 1000 W Moreno St. > Pensacola FL 32501 > Phone (850) 469-7718 > Fax (850) 434-4996 > > ----------------------------------------- > All electronic data transmissions originating from or sent to > Baptist Health Care Corporation (BHC) are subject to monitoring. > This message along with any attached data, are the confidential and > proprietary communications of BHC and are intended to be received > only by the individual or individuals to whom the message has been > addressed. If the reader of this message is not the intended > recipient, please take notice that any use, copying, printing, > forwarding or distribution of this message, in any form, is > strictly prohibited and may violate State or Federal Law. If you > have received this transmission in error, please delete or destroy > all copies of this message. For questions, contact the BHC Privacy > Officer at (850) 434-4472. Rev.10/07. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brian <@t> detangleit.com Wed Feb 2 22:24:31 2011 From: brian <@t> detangleit.com (Brian Wood) Date: Wed Feb 2 22:24:34 2011 Subject: [Histonet] slide labeler advice In-Reply-To: References: Message-ID: <069154DC-7FD0-4F45-BCF2-E53ADA857FC6@detangleit.com> Hi Liz, As a provider of on-demand-printing solutions I've worked closely with labs using Thermo SlideMate printers. I've noticed a couple of simple maintenace steps that may dramatically improve your printing results. First, clean the print head daily. To do this, press the Setup button, then press Load. This releases the takeup spool that keeps pressure on the ribbon. Now, open the door and loosen the ribbon where it wraps around the print head - that's the half gold/half silver rectangular bar and the print head is at the lowest point where it comes to a "V" shape. Wet a Kwik Wipe with alcohol and gently clean the print head. If you haven't done this in a while you'll want to clean it well the first time around. Get the Kwik Wipe good and wet (but not dripping) and dab the alcohol on, let it sit for a few seconds, then wipe dry. Do it a few times using a new clean wipe and get all around the "V" shape. Also wipe away any dust or dirt around the inside. After that initial cleaning, you should only need to do a quick "wipe it clean", but make it a part of daily maintenance at the start or end of each day. Before you close the door, wind the takeup spool to remove any slack in the tape. Second, remove excess tape from the takeup spool. As more tape winds up on the takeup spool the tension can become "spongy". This can cause the tape to crease which will leave a white line in the printed area. When you do your daily maintenance, just strip the day's tape from the takeup spool and re-thread it. Performing these steps daily should take 2 - 3 minutes and should keep print quality consistent. There are other things I've come across for programming and utilities for the SlideMate, etc. but too much to include here. Also, we provide our own print-on-demand software solution that works with the SlideMate and other label printers. Feel free to post additional questions, or contact me off line. Best Regards, Brian Brian Wood Detangle IT, Inc. 516 594-9344 brian@detangleit.com On Feb 2, 2011, at 5:56 PM, "Liz Chlipala" > wrote: Hello again We are in the market for a slide labeler. We currently have the SlideMate from Thermo Scientific. We have had it for about 2 years now and we are just not happy with the quality and consistency of the printing. We are a contract research lab and not a clinical lab. Entering data for each slide is not an option. The programming that comes with the Slide Mate is nice, except that no one has really spent the time to train us on it, we have figured it out a bit. We would want a labeler that we could create formats for different sample types, so all we would have to do is put the animal ID in and the printer would print all of the slides needed. Would like barcoding capabilities just incase we move to that in the future. Any advice or suggestions are appreciated. Vendors welcome also. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This message scanned by Reflexion Total Control From ratliffjack <@t> hotmail.com Wed Feb 2 23:35:19 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Feb 2 23:35:43 2011 Subject: [Histonet] Safranin O In-Reply-To: <7267A64D75F58241B577876D8A885631038FE474@msgebe41> References: <7267A64D75F58241B577876D8A885631038FE474@msgebe41> Message-ID: Have you tried Sanderson's Rapid Bone Stain? Try using it first (7 minutes at 60C), then rinse in dH2O (a few dip an dunks at 60C and blot dry), then counterstain with safranin O for 2-5 minutes (room temp - check intensity) and rinse in 100% EtOH (room temp - few dip and dunks and blot dry). If saf o is too intense, you should be able to differentiate a little with 95% EtOH first then do the 100% EtOH step. On a curious note, is there any particular reason why you are cutting thick sections and grinding? You should very easily be able to cut thin sections with a rotary microtome using a tungsten-carbide knife at 5 microns and then you would have greater staining flexibility. Call me if you want to discuss (317-281-1975). Jack On Feb 2, 2011, at 4:18 PM, "Herrick, James L. (Jim)" wrote: > Hello everyone, > > I am trying to stain for cartilage in mouse skull (cross-sectioning with > the brain in tact) and wanted to know if anyone might have a good > protocol for Safranin O / Fast green or Safranin O / von Kossa? The > tissue is embedded in MMA and was cut on a saw microtome and finished > down to an approximate 20 to 30 micron tissue thickness - 50 microns > including the glue layer (I glue a slide to the block before making the > cut and then grind and polish to finish). I tried Weigert's hematoxylin > / Safranin O / Fast Green ( I began with Weigert's Hematoxylin, followed > by Fast Green and finished with Safranin O), but the Safranin O seemed > to overpower the Fast Green (the calcified bone stained more red than > green). Any help on this issue would be greatly appreciated as always. > Thanks again. > > Jim > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rgarcia <@t> ugr.es Thu Feb 3 02:54:31 2011 From: rgarcia <@t> ugr.es (R.G. del Moral) Date: Thu Feb 3 02:54:52 2011 Subject: [Histonet] Looking for a procedure In-Reply-To: Message-ID: This pretty technique has been shown today: Gelatin foam cell blocks made from cytology fluid specimens, in: http://jcp.bmj.com/content/early/2011/02/01/jcp.2010.088542.full.html#ref-li st-1 Raimundo G. del Moral MD Departamento de Anatom?a Patol?gica Facultad de Medicina Avda. de Madrid, 12 18012 GRANADA SPAIN -----Mensaje original----- De: Bruce W Brodersen [mailto:bbroders@unlnotes.unl.edu] Enviado el: lunes, 31 de enero de 2011 23:11 Para: histonet@lists.utsouthwestern.edu Asunto: [Histonet] Looking for a procedure Looking for a procedure to embed tissue culture cells (speficially algae) in agar for frozen sections. Anyone have any? Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094 From Michele.Ellender <@t> hpa.org.uk Thu Feb 3 04:47:36 2011 From: Michele.Ellender <@t> hpa.org.uk (Michele Ellender) Date: Thu Feb 3 04:47:44 2011 Subject: [Histonet] 53BP1 and gamma-H2AX antibodies Message-ID: Good morning Histonet, Does anyone know of 53BP1 and gamma-H2AX antibodies that work in FFPE rat tissue (lung, liver, spleen, brain). Would appreciate any suggestions anyone might have, Best wishes, Michele Dr Michele Ellender Biological Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Health Protection Agency, Chilton, Didcot, Oxon. OX11 ORQ UK Phone: +44 (0)1235 822697 Fax: +44 (0)1235 833891 E-mail michele.ellender@hpa.org.uk ----------------------------------------- ************************************************************************** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of the HPA, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses, but please re-sweep any attachments before opening or saving. HTTP://www.HPA.org.uk ************************************************************************** From NSEARCY <@t> swmail.sw.org Thu Feb 3 07:47:38 2011 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Feb 3 07:47:44 2011 Subject: [Histonet] Question Message-ID: <4D4A5D9A.5D38.00EF.0@swmail.sw.org> In power outages ( bumps) does the Sakura cryostat resent micron settings? ( factory setting? ) I figure that this would be the fastest way to get an answer. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From sgoebel <@t> mirnarx.com Thu Feb 3 08:16:41 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Feb 3 08:16:43 2011 Subject: [Histonet] CSF for cytology In-Reply-To: <269c9413cd1d2f8f650037eeeb9e1c58.squirrel@webmail.integra.net> References: <269c9413cd1d2f8f650037eeeb9e1c58.squirrel@webmail.integra.net> Message-ID: Most of the time cell blocks are not made from CSF fluid? Usually they just want a diff-Quik cytospin and a PAP cytospin? I don't know if a cell block could even be made from CSF? I'm curious to hear responses... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katelin Lester Sent: Wednesday, February 02, 2011 7:54 PM To: histonet Subject: [Histonet] CSF for cytology Hi Histonet, We are going to be receiving some cerebrospinal fluid as party of an MS Panel for cytology. Our pathologist would like me to make a cell button and stain it with H&E and Diff Quik, but I wanted to check with you guys and see if there are any other stains that I might do with this case. Thank you for your help, Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Thu Feb 3 08:44:56 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Feb 3 08:44:54 2011 Subject: [Histonet] Histology Jobs in NY Message-ID: <2253314.1296744296565.JavaMail.cfservice@SL4APP1> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on several open positions with a fast paced and expanding speciality laboratory in Westchester County. This company is provides transportation directly from the city to their lab. They are a speciality pathology company offering anatomic, molecular, digital, and local testing services. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus (1st or 3rd shift or Per Diem) * Experienced Histotech - NYS licensed histotech (2nd shift or Per Diem) * Cyto Prep Technician * Mycology Technician - Per Diem * PCR Tech - 1st shift They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From alisha <@t> ka-recruiting.com Thu Feb 3 08:47:40 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Feb 3 08:47:39 2011 Subject: [Histonet] Looking for someone with muscle biopsy experience in NY Message-ID: <1797906548.1296744460288.JavaMail.cfservice@sl4app3> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must * Muscle/Nerve Technician - Must have muscle biopsy experience They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From jm.lapointe <@t> accellab.com Thu Feb 3 09:03:38 2011 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Thu Feb 3 09:03:42 2011 Subject: [Histonet] RE: Histonet Digest, Vol 87, Issue 5 In-Reply-To: <201102030216.p132GoiP007373@gateway5.lastspam.com> References: <201102030216.p132GoiP007373@gateway5.lastspam.com> Message-ID: Hi Shu, I would stay away from zinc fixatives if I were you. It does a very poor job of preserving tissue integrity. There's nothing too esoteric in the cells you need to stain, so formalin fixation should be fine for this. Jean-Martin Lapointe Accellab Inc ? ------------------------------ Message: 2 Date: Wed, 2 Feb 2011 13:28:47 -0500 From: "Chen, Shu-Cheng" Subject: [Histonet] Rat lung histology for mast cells and eosinophils To: "histonet@lists.utsouthwestern.edu" Message-ID: <5D62649615FAA6478F801A08D10E51855983C4A9AF@USCTMXP51003.merck.com> Content-Type: text/plain; charset="us-ascii" Hi, We are called to support an asthmatic rat lung project and need to stain for mast cells, eosinophils, neutrophils and possibly other inflammatory cells, such as Th2 cells etc. Any suggestions you can give us in terms of fixatives and special stains or IHC are very much appreciated. From my search, it seems that Carnoy's fixative with toluidine blue is the way to go for mast cells. But can one clearly differentiate eos from neuts with this fixative or a formalin fixed H&E stained lung section? If we need to do Trichrome, PAS, May Grunwald Giemsa and H&E etc. what would be the best fixative to accommodate these stains? BTW, I heard that formalin free Zn-fixative is good for IHC. Will it be good for all these stains above? Sorry for so many questions. It shows how little experience I have in this area. Thank you, Shu From AGleiberman <@t> cbiolabs.com Thu Feb 3 09:04:43 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Thu Feb 3 09:04:49 2011 Subject: [Histonet] RE: 53BP1 and gamma-H2AX antibodies In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C0F1D9B@cbiolabs05.CBiolabs.local> Rabbit anti-Phospho-Histone H2A.X (Ser139) Antibody #2577 from Cell Signaling works very well in FFPE(dilution 1:100 in immunofluorescence). Don't know good anti 53BP1. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Ellender Sent: Thursday, February 03, 2011 5:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 53BP1 and gamma-H2AX antibodies Good morning Histonet, Does anyone know of 53BP1 and gamma-H2AX antibodies that work in FFPE rat tissue (lung, liver, spleen, brain). Would appreciate any suggestions anyone might have, Best wishes, Michele Dr Michele Ellender Biological Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Health Protection Agency, Chilton, Didcot, Oxon. OX11 ORQ UK Phone: +44 (0)1235 822697 Fax: +44 (0)1235 833891 E-mail michele.ellender@hpa.org.uk ----------------------------------------- ************************************************************************** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of the HPA, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses, but please re-sweep any attachments before opening or saving. HTTP://www.HPA.org.uk ************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From ozgekrdnz <@t> gmail.com Thu Feb 3 09:13:45 2011 From: ozgekrdnz <@t> gmail.com (ozge karadeniz) Date: Thu Feb 3 09:13:48 2011 Subject: [Histonet] about histonet mailing list Message-ID: could you pls delete my e-mail adress from mailing list thanks ozge -- *Mutlu G?nler :) ?zge KARADEN?Z* From indytreegers <@t> sbcglobal.net Thu Feb 3 09:55:53 2011 From: indytreegers <@t> sbcglobal.net (indytreegers@sbcglobal.net) Date: Thu Feb 3 09:55:58 2011 Subject: [Histonet] CLIA classes? Message-ID: <203876.40821.qm@web81906.mail.mud.yahoo.com> Hi all, ? Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab? ? Thanks for any advice you can give me on this! Lyn L. Treeger, B.S.HT(ASCP) From jsands <@t> innovishealth.com Thu Feb 3 10:02:33 2011 From: jsands <@t> innovishealth.com (Sands, Jenn) Date: Thu Feb 3 10:03:28 2011 Subject: [Histonet] CLIA classes? In-Reply-To: <203876.40821.qm@web81906.mail.mud.yahoo.com> References: <203876.40821.qm@web81906.mail.mud.yahoo.com> Message-ID: <6F45795DD2B08F40A25F7EF45A7082BF129B01@turkey.medcampus.org> I would be interested in this information as well. Thank you. Jenn Sands, CT(ASCP)cm, HTL(ASCP)cm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of indytreegers@sbcglobal.net Sent: Thursday, February 03, 2011 9:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA classes? Hi all, Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab? Thanks for any advice you can give me on this! Lyn L. Treeger, B.S.HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From vavalos <@t> allergydermatology.com Thu Feb 3 10:56:06 2011 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Thu Feb 3 10:56:11 2011 Subject: FW: [Histonet] CLIA classes? Message-ID: <001b01cbc3c3$3fcbf000$bf63d000$@com> I work at at derm office and this would be great to know about if there was one. Please please please include me in the list of responses. Thanks! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sands, Jenn Sent: Thursday, February 03, 2011 9:03 AM To: indytreegers@sbcglobal.net; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA classes? I would be interested in this information as well. Thank you. Jenn Sands, CT(ASCP)cm, HTL(ASCP)cm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of indytreegers@sbcglobal.net Sent: Thursday, February 03, 2011 9:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA classes? Hi all, Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab? Thanks for any advice you can give me on this! Lyn L. Treeger, B.S.HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Thu Feb 3 10:59:50 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Thu Feb 3 10:57:26 2011 Subject: [Histonet] Her2 Fixation Requirement Responses/Thanks Message-ID: <354DF09D16744297B9752CDA1212FE38@biopath.local> Your information and suggestions helped me formulate a plan, so thank you to those who took the time and responded to my inquiry yesterday. Paula Lucas From Kim.Donadio <@t> bhcpns.org Thu Feb 3 11:42:55 2011 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Thu Feb 3 11:43:06 2011 Subject: [Histonet] Trace Gas companies In-Reply-To: <5f8d8d4e5cc7f642003d5b8ffbf96552.squirrel@webmail.integra.net> Message-ID: Thanks to all who gave the info. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Katelin Lester" 02/02/2011 08:11 PM To Kim.Donadio@bhcpns.org cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Trace Gas companies We just did ours this week! We get them from Environmental Monitoring Technology. http://www.emt-online.com/ Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > Hi Histonetters > > Can you all share with me where you get and send in your trace gas > analysis badges? > > Thanks a bunch! > > :-D > > > > > > > > Kim Donadio > Pathology Supervisor > Baptist Hospital > 1000 W Moreno St. > Pensacola FL 32501 > Phone (850) 469-7718 > Fax (850) 434-4996 > > ----------------------------------------- > All electronic data transmissions originating from or sent to > Baptist Health Care Corporation (BHC) are subject to monitoring. > This message along with any attached data, are the confidential and > proprietary communications of BHC and are intended to be received > only by the individual or individuals to whom the message has been > addressed. If the reader of this message is not the intended > recipient, please take notice that any use, copying, printing, > forwarding or distribution of this message, in any form, is > strictly prohibited and may violate State or Federal Law. If you > have received this transmission in error, please delete or destroy > all copies of this message. For questions, contact the BHC Privacy > Officer at (850) 434-4472. Rev.10/07. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sfeher <@t> CMC-NH.ORG Thu Feb 3 11:45:09 2011 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Feb 3 11:45:12 2011 Subject: [Histonet] slide labeler advice In-Reply-To: References: Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2466996A@exchange.cmc-nh.org> Hi Liz, We use Leica's IPC and IPS equipment and have found a simple solution to using them with our LIS. If you connect a computer (CPU) only, to each IPC or IPS you want to interface, you can use these units like any other printer that your LIS lists. We routinely print slides and cassettes automatically when a case is accessioned or on demand from any of our workstations. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, February 02, 2011 5:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide labeler advice Hello again We are in the market for a slide labeler. We currently have the SlideMate from Thermo Scientific. We have had it for about 2 years now and we are just not happy with the quality and consistency of the printing. We are a contract research lab and not a clinical lab. Entering data for each slide is not an option. The programming that comes with the Slide Mate is nice, except that no one has really spent the time to train us on it, we have figured it out a bit. We would want a labeler that we could create formats for different sample types, so all we would have to do is put the animal ID in and the printer would print all of the slides needed. Would like barcoding capabilities just incase we move to that in the future. Any advice or suggestions are appreciated. Vendors welcome also. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bob.nienhuis <@t> gmail.com Thu Feb 3 12:29:18 2011 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Thu Feb 3 12:29:23 2011 Subject: [Histonet] Formaldehyde monitor Message-ID: Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles From Rcartun <@t> harthosp.org Thu Feb 3 12:43:05 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 3 12:43:13 2011 Subject: [Histonet] Consult accessioning question Message-ID: <4D4AB0E8.7400.0077.1@harthosp.org> When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From victor <@t> pathology.washington.edu Thu Feb 3 12:59:53 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Feb 3 13:00:08 2011 Subject: [Histonet] Consult accessioning question In-Reply-To: <4D4AB0E8.7400.0077.1@harthosp.org> References: <4D4AB0E8.7400.0077.1@harthosp.org> Message-ID: <4D4AFB29.8070601@pathology.washington.edu> Richard, We use the date of accessioning as the DOS and we wouldn't accession it until we have received the specimen/materials. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/3/2011 10:43 AM, Richard Cartun wrote: > When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology& Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Thu Feb 3 13:06:14 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Feb 3 13:06:20 2011 Subject: [Histonet] Consult accessioning question In-Reply-To: <4D4AB0E8.7400.0077.1@harthosp.org> References: <4D4AB0E8.7400.0077.1@harthosp.org> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EBA807A@NADCWPMSGCMS03.hca.corpad.net> In our LIS system we put in "specimen date" or DOS and we also have a "Received date". My understanding, for insurance/billing purposes, you would need to include the original DOS, but you also need to be able to document when you actually received the specimen. Just my 2 cents!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 03, 2011 1:43 PM To: Histonet Subject: [Histonet] Consult accessioning question When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Thu Feb 3 13:10:44 2011 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Thu Feb 3 13:11:12 2011 Subject: [Histonet] Formaldehyde monitor In-Reply-To: Message-ID: Call Advanced Chemical Sensors Inc @ 561-338-3116 I think they are like $100 each. Last I looked anyway. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Bob Nienhuis Sent by: histonet-bounces@lists.utsouthwestern.edu 02/03/2011 12:29 PM Please respond to bob.nienhuis@gmail.com To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Formaldehyde monitor Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Wanda.Smith <@t> HCAhealthcare.com Thu Feb 3 13:12:42 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Feb 3 13:12:46 2011 Subject: [Histonet] Formaldehyde monitor In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EBA8097@NADCWPMSGCMS03.hca.corpad.net> Lab Safety Supply has the option to order the badges for formaldehyde and Xylene and when you order, you pre-pay for the analysis. Formalin is $476.00 for 5 monitors. Xylene is $259.00 for 4 monitors. They email you a very nice report for your file, and it only takes about a week to 10 days. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis Sent: Thursday, February 03, 2011 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde monitor Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Feb 3 13:45:25 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 3 13:45:45 2011 Subject: [Histonet] Consult accessioning question In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EBA807A@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B1E@is-e2k3.grhs.net> Same for us. You need both DOS from office as well as date received. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Thursday, February 03, 2011 1:06 PM To: Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Consult accessioning question In our LIS system we put in "specimen date" or DOS and we also have a "Received date". My understanding, for insurance/billing purposes, you would need to include the original DOS, but you also need to be able to document when you actually received the specimen. Just my 2 cents!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 03, 2011 1:43 PM To: Histonet Subject: [Histonet] Consult accessioning question When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Thu Feb 3 13:49:13 2011 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Feb 3 13:49:24 2011 Subject: [Histonet] Off Topic: researchers very funny In-Reply-To: <4D4889450200001A0005B3B9@GWIA2.umm.edu> References: <4D4889450200001A0005B3B9@GWIA2.umm.edu> Message-ID: <942FEC2A5A5B390A7D533E20@CDYwxp1931.ad.med.buffalo.edu> yes our lab received it last week! And yes we've been posting it to Facebook as well (as someone else on Histonet commented!) If the lady in the video never gets her PhD, she may want to consider the entertainment industry! --On Tuesday, February 01, 2011 10:29 PM -0500 Kimberly Tuttle wrote: > If this is a repost I apologize :) > > http://www.youtube.com/watch?v=Fl4L4M8m4d0&feature=player_embedded > > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this > e-mail. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From JWeems <@t> sjha.org Thu Feb 3 14:08:30 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Feb 3 14:08:34 2011 Subject: [Histonet] Consult accessioning question In-Reply-To: <4D4AB0E8.7400.0077.1@harthosp.org> References: <4D4AB0E8.7400.0077.1@harthosp.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081DE5924C@CHEXCMS10.one.ads.che.org> For Medicare, my understanding would be to use the DOS if less than 30 days, and to use the date pulled from archives if more than 30 days. At least that is the regs for additional tests. I think it would be the same for consult as well. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 03, 2011 13:43 To: Histonet Subject: [Histonet] Consult accessioning question When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From plucas <@t> biopath.org Thu Feb 3 14:38:01 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Thu Feb 3 14:35:38 2011 Subject: [Histonet] TTF-1/background staining Message-ID: How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA From Dorothy.L.Webb <@t> HealthPartners.Com Thu Feb 3 14:38:06 2011 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Feb 3 14:38:10 2011 Subject: [Histonet] Microwave processing Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43FD0CF299E6@HPEMX3.HealthPartners.int> Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork! Thanks! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From vavalos <@t> allergydermatology.com Thu Feb 3 15:10:48 2011 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Thu Feb 3 15:10:53 2011 Subject: FW: [Histonet] Formaldehyde monitor Message-ID: <000001cbc3e6$d4ae53c0$7e0afb40$@com> We use Advanced Chemical Sensors. Acsbadge.com is website. I also used to use Surgipath. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis Sent: Thursday, February 03, 2011 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde monitor Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 3 15:17:50 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 3 15:17:53 2011 Subject: [Histonet] Microwave processing In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43FD0CF299E6@HPEMX3.HealthPartners.int> Message-ID: <787421.30108.qm@web65712.mail.ac4.yahoo.com> Dorothy: You should?follow for MW processing the same procedure you follow for any?"traditional" tissue processing. What you have to document is that when you started to use the MW processing there was a documented QC to compare the results between the method you used and the new with the MW processor. Having to sign a QC after each run is absolutely unnecessary if the initial QC was done. Ren? J. ? ? --- On Thu, 2/3/11, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Microwave processing To: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, February 3, 2011, 3:38 PM Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run?? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork! Thanks! Dorothy Webb ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Thu Feb 3 15:57:22 2011 From: JWatson <@t> gnf.org (James Watson) Date: Thu Feb 3 15:57:36 2011 Subject: [Histonet] Temporary histology position in San Diego Ca. Message-ID: GNF is currently seeking a temporary Scientific Associate to join the Histology group, for the beginning of March 2011 through the end of July 2011. Perform necropsies, tissue processing, slide sectioning, routine staining, special staining, immunohistochemical staining and complex procedures necessary in preparing specimens of animal tissue in a research environment. Qualifications: Associate's or B.S. degree in a biological science or completion of a NAACLS accredited School of Histotechnology is required. American Society of Clinical Pathologist (ASCP) certification as a HT or HTL is required. Applicants must demonstrate the potential ability to perform the essential functions of the job as outlined in the position description. The employee must have 2-5 years of experience in the Histology lab performing animal techniques, a wide variety of manual histochemical and enzymatic staining, automated and manual immunohistochemistry, and automated and manual in-situ hybridization. Essential Functions: 1. Identifies significant tissue elements microscopically to determine quality of staining. 2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. 3. Performs Microtomy on rotary microtome, cryostat, and be able to use a sliding microtome. 4. Prepares dyes and solutions in order to perform routine, special, and complex procedures. 5. Have experience in histochemical stains and enzymatic histochemical stains. Be able to trouble shoot and correct problems with histochemical stains. Have experience in Immunohistochemical staining and other advanced histological procedures. 6. Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. 7. Operate Slide scanning Instrumentation. Interested candidates please send a copy of your CV with a summary of research experience, and three references. Job Code: JW10-013 Contact:E-mail: j...@gnf.org James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From DSiena <@t> statlab.com Thu Feb 3 16:02:22 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu Feb 3 16:02:27 2011 Subject: [Histonet] TTF-1/background staining In-Reply-To: References: Message-ID: Hi Paula, I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much? thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010? x229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, February 03, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TTF-1/background staining How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Thu Feb 3 16:54:25 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Feb 3 17:08:11 2011 Subject: [Histonet] Her2 Fixation Requirement In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164081DE58F2F@CHEXCMS10.one.ads.che.org> Message-ID: Does anyone have issues with inking the specimen after it has been placed in formalin? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Wednesday, February 02, 2011 12:59 PM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement We have the nurse document the time of removal on the requisition. The next requisition update will have a spot for this. They have been very cooperative and do a good job. In addition, you must document the "cold ischmic time" - that is the time from removal until time in formalin. This is important when the specimen goes for xray or whatever. So there is a removal time, an into formalin time and an out of formalin time. Then if we don't have time allowed to meet the fixation time with the regular, it is put on a late processor, if we have one available, or it is held overnight. And if it has to come off on Sunday, we have a med tech remove it from the processor and it waits for us to come on Monday to embed it. The pathologists document all this time in the report. Best! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, February 02, 2011 12:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2 Fixation Requirement Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From ncosenza <@t> siumed.edu Thu Feb 3 17:44:39 2011 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Thu Feb 3 17:44:43 2011 Subject: [Histonet] Karnovsky and Roots stain Message-ID: <4D4B3DE7.70203@siumed.edu> I am looking into a project involving motor end plate staining. Literature that I've found continually references Karnovsky and Roots from the 60s. However the papers are not supplying all the details. Does anyone do AchE staining by this method on fresh frozen, unfixed tissue sections? If so, can I get a more detailed protocol (fixation steps, washes, etc)? From Tony_Reilly <@t> health.qld.gov.au Thu Feb 3 18:17:27 2011 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Thu Feb 3 18:17:43 2011 Subject: [Histonet] TTF-1/background staining In-Reply-To: References: Message-ID: <4D4BD237.411C.0039.0@health.qld.gov.au> Hi Paula I do not believe that RTU Abs really exist. No company can produce an antibody that is suitable for use by all laboratories taking in to consideration how tissue can be handled differently from lab to lab. I have used many RTU Abs which I have had to dilute to get optimal staining with Cam 5.2 a good example. On the Bond I was using it at 1:25. try titreing your Ab and see if that will reduce/eliminate your background. regards Tony >>> Debra Siena 4/02/2011 8:02 am >>> Hi Paula, I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much? thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, February 03, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TTF-1/background staining How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From marktarango <@t> gmail.com Thu Feb 3 20:39:15 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Feb 3 20:39:21 2011 Subject: [Histonet] TTF-1/background staining In-Reply-To: References: Message-ID: Hi Paula, You mentioned the staining in a liver specimen using the cell marque antibody against TTF-1. The clone named 8G7G3/1 that cell marque sells is known to stain liver cells and liver cancer. Your pathologist might be interested in knowing that. Here is a reference you can give him: http://www.nordiqc.org/Run-23-B5/Assessment/Assessment-TTF-1.htm It also lists another clone that doesn't cross react with liver. Mark On Thu, Feb 3, 2011 at 12:38 PM, Paula Lucas wrote: > How can I eliminate the background or as you say, non-specific staining? > > > > I'm no expert here, I admit, and so I'm asking for your help and > suggestions. I have searched the Histonet archives, and a lot of what I'm > seeing deals with animal or rodent tissues, and a lot of it was confusing > to > me. > > > > To give you a little background info: > > We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell > Marque. We were having issues with this marker from Lab Vision, so we > switched to TTF-1 a while ago. We use the UltraVision LP detection kit > from > Lab Vision. It's a polymer driven detection kit. > > > > The antibody is a ready to use, and we have the time set at 30 minutes. > > > > We were getting the background staining on a liver specimen last week, and > we also had this problem today on a lung case. My doctor is getting > frustrated, and wants me to do something about this, and to repeat the > TTF-1 > stain on the lung, so if someone can give me some suggestions to try, I'm > ready to try them. > > > > Thanks in advance, > > Paula > > Lab Manager > > Bio-Path Medical Group > > Fountain Valley, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From An.Eerdekens <@t> med.kuleuven.be Fri Feb 4 02:19:53 2011 From: An.Eerdekens <@t> med.kuleuven.be (An Eerdekens) Date: Fri Feb 4 02:20:03 2011 Subject: [Histonet] thionin staining Message-ID: <85BD21DE1A6DE94D9A30A2F2C1D2328B3D547D231E@ICTS-S-EXC4-CA.luna.kuleuven.be> Dear all, I have problems with thionin staining. I am using this on paraffin slices of hypothalami. In the past, I never experienced problems, but the last times, the staining is washed off by going through the ethanol after staining (50%, 70%, 90%, 100%). I tried already a few protocols, but every time the same result. Does anybody have experience with it? regards An Eerdekens From jdooley2008 <@t> yahoo.com Fri Feb 4 04:19:39 2011 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Fri Feb 4 04:19:43 2011 Subject: [Histonet] Cre protocol-fresh frozen section Message-ID: <340255.99220.qm@web45904.mail.sp1.yahoo.com> Good Afternoon, I would like to do fluoresent staining for Cre expression in thymus. Does anyone have protocol for doing this. I know Covance sells a Cre antibody. Best regards, James From erika <@t> mapslab.net Fri Feb 4 04:55:08 2011 From: erika <@t> mapslab.net (erika@mapslab.net) Date: Fri Feb 4 04:55:12 2011 Subject: =?UTF-8?Q?RE:=20[Histonet]=20CLIA=20classes=3F?= In-Reply-To: <6F45795DD2B08F40A25F7EF45A7082BF129B01@turkey.medcampus.org> References: <203876.40821.qm@web81906.mail.mud.yahoo.com> <6F45795DD2B08F40A25F7EF45A7082BF129B01@turkey.medcampus.org> Message-ID: <1296816908.91084963@192.168.4.58> I would be interested in this as well. ?I supervise a dermpath lab and have for five years. ?I have always passed with no deficiencies on my inspections. ?The inspector always tells me that I document to much stuff. Thanks, Erika -----Original Message----- From: "Sands, Jenn" Sent: Thursday, February 3, 2011 11:02am To: indytreegers@sbcglobal.net, Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA classes? I would be interested in this information as well. Thank you. Jenn Sands, CT(ASCP)cm, HTL(ASCP)cm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of indytreegers@sbcglobal.net Sent: Thursday, February 03, 2011 9:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA classes? Hi all, Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab? Thanks for any advice you can give me on this! Lyn L. Treeger, B.S.HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Fri Feb 4 05:37:09 2011 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Feb 4 05:40:28 2011 Subject: [Histonet] Thionin staining Message-ID: An - You didn't mention what cells you were staining, but I assume it's mast cells. Thionin stains are very pH dependent. For mast cells, the pH should be 1. At that pH only mast cells stain. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 From baier.patricia <@t> marshfieldclinic.org Fri Feb 4 08:14:39 2011 From: baier.patricia <@t> marshfieldclinic.org (Baier, Patricia) Date: Fri Feb 4 08:14:46 2011 Subject: [Histonet] Re: Histonet Digest, Vol 87, Issue 7 Message-ID: <201102041414.p14EEYH9022455@spamfilt> Patty view utube if you can. ------Original Message------ From: "histonet-request@lists.utsouthwestern.edu" Date: Fri Feb 04, 2011 -- 04:58:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Formaldehyde monitor (Bob Nienhuis) 2. Consult accessioning question (Richard Cartun) 3. Re: Consult accessioning question (Victor Tobias) 4. RE: Consult accessioning question (Wanda.Smith@HCAhealthcare.com) 5. Re: Formaldehyde monitor (Kim.Donadio@bhcpns.org) 6. RE: Formaldehyde monitor (Wanda.Smith@HCAhealthcare.com) 7. RE: Consult accessioning question (Mike Pence) 8. Re: Off Topic: researchers very funny (Merced M Leiker) 9. RE: Consult accessioning question (Weems, Joyce) 10. TTF-1/background staining (Paula Lucas) 11. Microwave processing (Webb, Dorothy L) 12. FW: [Histonet] Formaldehyde monitor (Vanessa Avalos) 13. Re: Microwave processing (Rene J Buesa) 14. Temporary histology position in San Diego Ca. (James Watson) 15. RE: TTF-1/background staining (Debra Siena) 16. RE: Her2 Fixation Requirement (Rathborne, Toni) 17. Karnovsky and Roots stain (Nicole Cosenza) 18. RE: TTF-1/background staining (Anthony Reilly) 19. Re: TTF-1/background staining (Mark Tarango) 20. thionin staining (An Eerdekens) 21. Cre protocol-fresh frozen section (James Dooley) 22. RE: CLIA classes? (erika@mapslab.net) ---------------------------------------------------------------------- Message: 1 Date: Thu, 3 Feb 2011 10:29:18 -0800 From: Bob Nienhuis Subject: [Histonet] Formaldehyde monitor To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles ------------------------------ Message: 2 Date: Thu, 03 Feb 2011 13:43:05 -0500 From: "Richard Cartun" Subject: [Histonet] Consult accessioning question To: "Histonet" Message-ID: <4D4AB0E8.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 3 Date: Thu, 03 Feb 2011 10:59:53 -0800 From: Victor Tobias Subject: Re: [Histonet] Consult accessioning question To: histonet@lists.utsouthwestern.edu Message-ID: <4D4AFB29.8070601@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Richard, We use the date of accessioning as the DOS and we wouldn't accession it until we have received the specimen/materials. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/3/2011 10:43 AM, Richard Cartun wrote: > When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology& Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 3 Feb 2011 13:06:14 -0600 From: Subject: RE: [Histonet] Consult accessioning question To: , Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EBA807A@NADCWPMSGCMS03.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" In our LIS system we put in "specimen date" or DOS and we also have a "Received date". My understanding, for insurance/billing purposes, you would need to include the original DOS, but you also need to be able to document when you actually received the specimen. Just my 2 cents!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 03, 2011 1:43 PM To: Histonet Subject: [Histonet] Consult accessioning question When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 3 Feb 2011 13:10:44 -0600 From: Kim.Donadio@bhcpns.org Subject: Re: [Histonet] Formaldehyde monitor To: bob.nienhuis@gmail.com Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Call Advanced Chemical Sensors Inc @ 561-338-3116 I think they are like $100 each. Last I looked anyway. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Bob Nienhuis Sent by: histonet-bounces@lists.utsouthwestern.edu 02/03/2011 12:29 PM Please respond to bob.nienhuis@gmail.com To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Formaldehyde monitor Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 6 Date: Thu, 3 Feb 2011 13:12:42 -0600 From: Subject: RE: [Histonet] Formaldehyde monitor To: , Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EBA8097@NADCWPMSGCMS03.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Lab Safety Supply has the option to order the badges for formaldehyde and Xylene and when you order, you pre-pay for the analysis. Formalin is $476.00 for 5 monitors. Xylene is $259.00 for 4 monitors. They email you a very nice report for your file, and it only takes about a week to 10 days. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis Sent: Thursday, February 03, 2011 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde monitor Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 3 Feb 2011 13:45:25 -0600 From: "Mike Pence" Subject: RE: [Histonet] Consult accessioning question To: , , Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B1E@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" Same for us. You need both DOS from office as well as date received. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Thursday, February 03, 2011 1:06 PM To: Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Consult accessioning question In our LIS system we put in "specimen date" or DOS and we also have a "Received date". My understanding, for insurance/billing purposes, you would need to include the original DOS, but you also need to be able to document when you actually received the specimen. Just my 2 cents!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 03, 2011 1:43 PM To: Histonet Subject: [Histonet] Consult accessioning question When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 03 Feb 2011 14:49:13 -0500 From: Merced M Leiker Subject: Re: [Histonet] Off Topic: researchers very funny To: Kimberly Tuttle , histonet@lists.utsouthwestern.edu Message-ID: <942FEC2A5A5B390A7D533E20@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed yes our lab received it last week! And yes we've been posting it to Facebook as well (as someone else on Histonet commented!) If the lady in the video never gets her PhD, she may want to consider the entertainment industry! --On Tuesday, February 01, 2011 10:29 PM -0500 Kimberly Tuttle wrote: > If this is a repost I apologize :) > > http://www.youtube.com/watch?v=Fl4L4M8m4d0&feature=player_embedded > > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this > e-mail. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 9 Date: Thu, 3 Feb 2011 15:08:30 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Consult accessioning question To: Richard Cartun , Histonet Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081DE5924C@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" For Medicare, my understanding would be to use the DOS if less than 30 days, and to use the date pulled from archives if more than 30 days. At least that is the regs for additional tests. I think it would be the same for consult as well. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 03, 2011 13:43 To: Histonet Subject: [Histonet] Consult accessioning question When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 10 Date: Thu, 3 Feb 2011 12:38:01 -0800 From: "Paula Lucas" Subject: [Histonet] TTF-1/background staining To: Message-ID: Content-Type: text/plain; charset="us-ascii" How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ------------------------------ Message: 11 Date: Thu, 3 Feb 2011 14:38:06 -0600 From: "Webb, Dorothy L" Subject: [Histonet] Microwave processing To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43FD0CF299E6@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork! Thanks! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 12 Date: Thu, 3 Feb 2011 14:10:48 -0700 From: "Vanessa Avalos" Subject: FW: [Histonet] Formaldehyde monitor To: Message-ID: <000001cbc3e6$d4ae53c0$7e0afb40$@com> Content-Type: text/plain; charset="us-ascii" We use Advanced Chemical Sensors. Acsbadge.com is website. I also used to use Surgipath. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis Sent: Thursday, February 03, 2011 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde monitor Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Thu, 3 Feb 2011 13:17:50 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Microwave processing To: "'histonet@lists.utsouthwestern.edu'" , Dorothy LWebb Message-ID: <787421.30108.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dorothy: You should follow for MW processing the same procedure you follow for any "traditional" tissue processing. What you have to document is that when you started to use the MW processing there was a documented QC to compare the results between the method you used and the new with the MW processor. Having to sign a QC after each run is absolutely unnecessary if the initial QC was done. Reni J. --- On Thu, 2/3/11, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Microwave processing To: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, February 3, 2011, 3:38 PM Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork! Thanks! Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 3 Feb 2011 13:57:22 -0800 From: James Watson Subject: [Histonet] Temporary histology position in San Diego Ca. To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" GNF is currently seeking a temporary Scientific Associate to join the Histology group, for the beginning of March 2011 through the end of July 2011. Perform necropsies, tissue processing, slide sectioning, routine staining, special staining, immunohistochemical staining and complex procedures necessary in preparing specimens of animal tissue in a research environment. Qualifications: Associate's or B.S. degree in a biological science or completion of a NAACLS accredited School of Histotechnology is required. American Society of Clinical Pathologist (ASCP) certification as a HT or HTL is required. Applicants must demonstrate the potential ability to perform the essential functions of the job as outlined in the position description. The employee must have 2-5 years of experience in the Histology lab performing animal techniques, a wide variety of manual histochemical and enzymatic staining, automated and manual immunohistochemistry, and automated and manual in-situ hybridization. Essential Functions: 1. Identifies significant tissue elements microscopically to determine quality of staining. 2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. 3. Performs Microtomy on rotary microtome, cryostat, and be able to use a sliding microtome. 4. Prepares dyes and solutions in order to perform routine, special, and complex procedures. 5. Have experience in histochemical stains and enzymatic histochemical stains. Be able to trouble shoot and correct problems with histochemical stains. Have experience in Immunohistochemical staining and other advanced histological procedures. 6. Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. 7. Operate Slide scanning Instrumentation. Interested candidates please send a copy of your CV with a summary of research experience, and three references. Job Code: JW10-013 Contact:E-mail: j...@gnf.org James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org ------------------------------ Message: 15 Date: Thu, 3 Feb 2011 16:02:22 -0600 From: Debra Siena Subject: RE: [Histonet] TTF-1/background staining To: Paula Lucas , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Paula, I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much? thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, February 03, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TTF-1/background staining How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 3 Feb 2011 17:54:25 -0500 From: "Rathborne, Toni" Subject: RE: [Histonet] Her2 Fixation Requirement To: "Weems, Joyce" , "Paula Lucas" , Message-ID: Content-Type: text/plain; charset="utf-8" Does anyone have issues with inking the specimen after it has been placed in formalin? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Wednesday, February 02, 2011 12:59 PM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement We have the nurse document the time of removal on the requisition. The next requisition update will have a spot for this. They have been very cooperative and do a good job. In addition, you must document the "cold ischmic time" - that is the time from removal until time in formalin. This is important when the specimen goes for xray or whatever. So there is a removal time, an into formalin time and an out of formalin time. Then if we don't have time allowed to meet the fixation time with the regular, it is put on a late processor, if we have one available, or it is held overnight. And if it has to come off on Sunday, we have a med tech remove it from the processor and it waits for us to come on Monday to embed it. The pathologists document all this time in the report. Best! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, February 02, 2011 12:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2 Fixation Requirement Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 17 Date: Thu, 03 Feb 2011 17:44:39 -0600 From: Nicole Cosenza Subject: [Histonet] Karnovsky and Roots stain To: histonet@lists.utsouthwestern.edu Message-ID: <4D4B3DE7.70203@siumed.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I am looking into a project involving motor end plate staining. Literature that I've found continually references Karnovsky and Roots from the 60s. However the papers are not supplying all the details. Does anyone do AchE staining by this method on fresh frozen, unfixed tissue sections? If so, can I get a more detailed protocol (fixation steps, washes, etc)? ------------------------------ Message: 18 Date: Fri, 04 Feb 2011 10:17:27 +1000 From: "Anthony Reilly" Subject: RE: [Histonet] TTF-1/background staining To: "Paula Lucas" , "histonet@lists.utsouthwestern.edu" , "Debra Siena" Message-ID: <4D4BD237.411C.0039.0@health.qld.gov.au> Content-Type: text/plain; charset="us-ascii" Hi Paula I do not believe that RTU Abs really exist. No company can produce an antibody that is suitable for use by all laboratories taking in to consideration how tissue can be handled differently from lab to lab. I have used many RTU Abs which I have had to dilute to get optimal staining with Cam 5.2 a good example. On the Bond I was using it at 1:25. try titreing your Ab and see if that will reduce/eliminate your background. regards Tony >>> Debra Siena 4/02/2011 8:02 am >>> Hi Paula, I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much? thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, February 03, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TTF-1/background staining How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. 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Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** ------------------------------ Message: 19 Date: Thu, 3 Feb 2011 18:39:15 -0800 From: Mark Tarango Subject: Re: [Histonet] TTF-1/background staining To: Paula Lucas Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Paula, You mentioned the staining in a liver specimen using the cell marque antibody against TTF-1. The clone named 8G7G3/1 that cell marque sells is known to stain liver cells and liver cancer. Your pathologist might be interested in knowing that. Here is a reference you can give him: http://www.nordiqc.org/Run-23-B5/Assessment/Assessment-TTF-1.htm It also lists another clone that doesn't cross react with liver. Mark On Thu, Feb 3, 2011 at 12:38 PM, Paula Lucas wrote: > How can I eliminate the background or as you say, non-specific staining? > > > > I'm no expert here, I admit, and so I'm asking for your help and > suggestions. I have searched the Histonet archives, and a lot of what I'm > seeing deals with animal or rodent tissues, and a lot of it was confusing > to > me. > > > > To give you a little background info: > > We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell > Marque. We were having issues with this marker from Lab Vision, so we > switched to TTF-1 a while ago. We use the UltraVision LP detection kit > from > Lab Vision. It's a polymer driven detection kit. > > > > The antibody is a ready to use, and we have the time set at 30 minutes. > > > > We were getting the background staining on a liver specimen last week, and > we also had this problem today on a lung case. My doctor is getting > frustrated, and wants me to do something about this, and to repeat the > TTF-1 > stain on the lung, so if someone can give me some suggestions to try, I'm > ready to try them. > > > > Thanks in advance, > > Paula > > Lab Manager > > Bio-Path Medical Group > > Fountain Valley, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 20 Date: Fri, 4 Feb 2011 09:19:53 +0100 From: An Eerdekens Subject: [Histonet] thionin staining To: "histonet@lists.utsouthwestern.edu" Message-ID: <85BD21DE1A6DE94D9A30A2F2C1D2328B3D547D231E@ICTS-S-EXC4-CA.luna.kuleuven.be> Content-Type: text/plain; charset="iso-8859-1" Dear all, I have problems with thionin staining. I am using this on paraffin slices of hypothalami. In the past, I never experienced problems, but the last times, the staining is washed off by going through the ethanol after staining (50%, 70%, 90%, 100%). I tried already a few protocols, but every time the same result. Does anybody have experience with it? regards An Eerdekens ------------------------------ Message: 21 Date: Fri, 4 Feb 2011 02:19:39 -0800 (PST) From: James Dooley Subject: [Histonet] Cre protocol-fresh frozen section To: histonet@lists.utsouthwestern.edu Message-ID: <340255.99220.qm@web45904.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Good Afternoon, I would like to do fluoresent staining for Cre expression in thymus. Does anyone have protocol for doing this. I know Covance sells a Cre antibody. Best regards, James ------------------------------ Message: 22 Date: Fri, 4 Feb 2011 05:55:08 -0500 (EST) From: erika@mapslab.net Subject: RE: [Histonet] CLIA classes? To: "Sands, Jenn" Cc: histonet@lists.utsouthwestern.edu Message-ID: <1296816908.91084963@192.168.4.58> Content-Type: text/plain;charset=UTF-8 I would be interested in this as well. B I supervise a dermpath lab and have for five years. B I have always passed with no deficiencies on my inspections. B The inspector always tells me that I document to much stuff. Thanks, Erika -----Original Message----- From: "Sands, Jenn" Sent: Thursday, February 3, 2011 11:02am To: indytreegers@sbcglobal.net, Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA classes? I would be interested in this information as well. Thank you. Jenn Sands, CT(ASCP)cm, HTL(ASCP)cm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of indytreegers@sbcglobal.net Sent: Thursday, February 03, 2011 9:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA classes? Hi all, Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab? Thanks for any advice you can give me on this! Lyn L. Treeger, B.S.HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 87, Issue 7 *************************************** ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From plucas <@t> biopath.org Fri Feb 4 08:21:04 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Feb 4 08:18:42 2011 Subject: [Histonet] TTF-1/background staining In-Reply-To: References: Message-ID: Hugh thanks for your reply and I'll type my answers in blue below after the questions: _____ From: Hugh Luk [mailto:hlukey@msn.com] Sent: Thursday, February 03, 2011 6:21 PM To: plucas@biopath.org Subject: RE: [Histonet] TTF-1/background staining Paula, Incomplete IHC staining has many factors. 1) What type of antigen retrieval are you using? We use the Lab Vision's Citrate Buffer Ph 6 Can you use a high pH solution instead? Unmasking hard epitopes (TTF-1 can be stubborn) will look better under a higher pH AR (or TR for Dako users). Are you re-using your solution? Please say "No" "NO" or at least "not often." Lastly how are your retrieving? We use Biocare's Decloaking Chamber, basically a pressure cooker Pressure HIER or rolling boil methods work best for TTF1. 2) What type of buffer are you using. We use TBS with Tween added: a small capful to a 5 liter container TBS or PBS should both work. Is it pH'd correctly? Since you automate, does your buffer contain Tween 20? Too much or too little could ruin your day. 3) Primary antibody. Concentrate? It's a predilute Could your diluent (solution) be faulty or your titre incorrect? Call or email Cell Marque to help you with this? I did call Cell Marque and they gave me some suggestions such as using an EDTA based retrieval solution and to reduce the antibody time to 15 minutes, and to also increase the blocker from 5 to 10 minutes Another email from Tony asks about dilution too, and hints at problems with the anitbody (AR). I use Dako's version of TTF-1 for human tissues, and I have not seen a problem. Perhaps you could call Dako and procure a free sample? I'll try that 4) Fixation. TTF-1 isn't known to be fixation-dependent, but how long are your tissues sitting in formalin before processing? It depends.we receive samples from the hospital, and so it can vary. The smaller samples are in formalin at least 6 hours: includes the sample sitting in the formalin container waiting for gross, plus then the processor time Problems with over or under fixation could cause your description. Also, what fixative are you using? We use 10% Formalin I have heard various fixatives being used on liver biopsies: Carnoy's, Helly's, Hollende's, Zinc formalin, ect. If you are using another fixative, can you go back to 10% neutral buffered formalin? 5) Tissue processor. How is your tissue processor? It's a VIP and the paraffin is checked at 60 degrees Could your paraffin be overheating? 6) Detection system. I have simply never tried your kit, but I will say several antibodies from Thermo have worked well. Thermo lists their polymer system as non-avidin-biotin based, and should be ultra sensitive. If it works on everything else (especially stains that visualize the nucleus), I would say your kit is not the problem. But it still might not like your antibody. For example, the nuclear epitope is rather small and Dako's polymer kit (Envision plus) was a large bio-synthesized molecule that many people thought would sterically hinder a good percentage of binding, reducing the signal. Don't know if that's true, it works okay for us. 7) 30 minute primary incubation should be fine. Is there any chance your slides are drying while incubating? I'm not 100% sure..the slides look to me like they are not drying out. We apply the buffer as soon as we place the slides on the machine, and then we keep the cover to the machine closed while it's running, so I'm assuming the slides aren't drying out We sometimes put paper towels on the bottom of the stainer to assure the chamber is not drying out our slides (but that's only when we get REALLY paranoid). 8) Liver. Is dirty. Debbie has a point that the liver may be working against you. You could try a protein block, or something that Thermo may suggest to deter background. Do you ink your liver biopsies? No we don't ink the liver bx's Davidson's inks used to give us some problems with IHC on small tissues (so we went to marking with eosin or hematoxylin). 9) Liver does not normally stain TTF-1. HCC's should be negative. Lung would stain well (unless your "Lung" tissue is also a metastisis), but for example breast mets could be focal and weak to diffuse and strong. It depends on the tumor. Could the tumor be of this type? I will ask the pathologist this question Good luck and feel free to email me if something I wrote needs to be clarified. I apologize for the rambling on... don't apologize! I really appreciate this Hugh Cancer Research center of Hawaii > From: DSiena@statlab.com > To: plucas@biopath.org; histonet@lists.utsouthwestern.edu > Date: Thu, 3 Feb 2011 16:02:22 -0600 > Subject: RE: [Histonet] TTF-1/background staining > CC: > > Hi Paula, > > I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much? thanks > > Debbie Siena HT(ASCP)QIHC > Technical Manager | StatLab Medical Products > Direct: 972-436-1010 x229 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas > Sent: Thursday, February 03, 2011 2:38 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] TTF-1/background staining > > How can I eliminate the background or as you say, non-specific staining? > > > > I'm no expert here, I admit, and so I'm asking for your help and > suggestions. I have searched the Histonet archives, and a lot of what I'm > seeing deals with animal or rodent tissues, and a lot of it was confusing to > me. > > > > To give you a little background info: > > We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell > Marque. We were having issues with this marker from Lab Vision, so we > switched to TTF-1 a while ago. We use the UltraVision LP detection kit from > Lab Vision. It's a polymer driven detection kit. > > > > The antibody is a ready to use, and we have the time set at 30 minutes. > > > > We were getting the background staining on a liver specimen last week, and > we also had this problem today on a lung case. My doctor is getting > frustrated, and wants me to do something about this, and to repeat the TTF-1 > stain on the lung, so if someone can give me some suggestions to try, I'm > ready to try them. > > > > Thanks in advance, > > Paula > > Lab Manager > > Bio-Path Medical Group > > Fountain Valley, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhotaks <@t> mcmaster.ca Fri Feb 4 09:17:49 2011 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Fri Feb 4 09:18:15 2011 Subject: [Histonet] Bond Refine Red for manual IHC Message-ID: Hello all, I wonder if anyone is using the Bond Polymer Refine Red Detection kit (cat # DS390, Leica) for manual IHC. I've seen some beautiful staining, the technical specialist at Leica, however, told me it is only for Leica automatic stainers. It is an AP stain. Has anybody tried it? Thanks a lot, Sarka Lhotak McMaster University Hamilton, Ontario, Canada From Ronald.Houston <@t> nationwidechildrens.org Fri Feb 4 09:39:11 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Feb 4 09:39:20 2011 Subject: [Histonet] Bond Refine Red for manual IHC In-Reply-To: References: Message-ID: Yes, we've used both the AP Red and DAB Refine kits manually with comparable results to the automation Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarka Lhotak Sent: Friday, February 04, 2011 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bond Refine Red for manual IHC Hello all, I wonder if anyone is using the Bond Polymer Refine Red Detection kit (cat # DS390, Leica) for manual IHC. I've seen some beautiful staining, the technical specialist at Leica, however, told me it is only for Leica automatic stainers. It is an AP stain. Has anybody tried it? Thanks a lot, Sarka Lhotak McMaster University Hamilton, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From alisha <@t> ka-recruiting.com Fri Feb 4 09:45:33 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Fri Feb 4 09:45:33 2011 Subject: [Histonet] Looking for someone with muscle biopsy experience in NY Message-ID: <521131655.1296834333558.JavaMail.cfservice@sl4app2> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must * Muscle/Nerve Technician - Must have muscle biopsy experience They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From mw <@t> personifysearch.com Fri Feb 4 10:07:02 2011 From: mw <@t> personifysearch.com (Matt Ward) Date: Fri Feb 4 10:07:09 2011 Subject: [Histonet] Histology Training Specialist Based in the Chicago Area Message-ID: <5dc7282795ecbaa1145dc41521ec5021@mail.gmail.com> Good Morning Histonet! We currently have a very strong Histology Training Specialist opportunity with a World Leader in Histology equipment. This position would be based out of the company?s Corporate Office in the Chicago area and offers an outstanding opportunity for career growth. Please e-mail resumes to mw@personifysearch.com Thanks! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com *Histology Training Specialist * *The Company:* Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *The Opportunity:* The company currently has an opening for a Histology Training Specialist. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: Based on Experience Other: Full benefits - 401k program/matching *Primary Responsibilities:* The primary responsibility of this role will be to provide technical phone support by answering questions, troubleshooting problems, logging and closing complaint files and escalating major issues to appropriate company personnel. This role will also provide technical training on specified products in the company's newly constructed state-of-the-art Customer Support Laboratory. Training programs are designed for small groups to ensure maximum customer learning and satisfaction. Additional Responsibilities: - Provide product and applications phone support to end-users, field personnel and dealers for all product lines - Log all calls into Customer Support Database - Participate in development of training materials and conduct classes and labs for customers, employees and others as needed - Serve as technical liaison to Customer Service/Field Service/Product Management departments *Education and Experience Required:* Ability to interact with various people in a calm and positive fashion and the ability to effectively communicate information to groups of participants is required. Experience with data entry, MS Office programs (PowerPoint, Lotus Notes, Word) is also required. HT/HTL/QIHC (ASCP) is helpful but not required. From mmorales <@t> adrian.edu Fri Feb 4 10:36:55 2011 From: mmorales <@t> adrian.edu (Marti Morales) Date: Fri Feb 4 10:37:00 2011 Subject: [Histonet] basic start-up fluorescence microscope Message-ID: <4D4C2B27.5060406@adrian.edu> Hello, Can anyone please recommend a basic laboratory start-up fluorescence microscope ( DIC a must; green and red channels would be nice) ? Kind regards, Marti From POWELL_SA <@t> mercer.edu Fri Feb 4 10:37:44 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Feb 4 10:37:52 2011 Subject: [Histonet] Weekly reminder Message-ID: <9BF995BC0E47744E9673A41486E24EE22DBB186B1E@MERCERMAIL.MercerU.local> Hi Georgia, Alabama, ALL histotechs, The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge. The deadline for making hotel reservations is March 1, 2011 so that gives you a month to make your plans to attend, don't delay. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The complete program can be downloaded from our website at this link: www.histosearch.com/gsh> then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form. The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance. Come TRANSFORM yourselves. Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From Kim.Donadio <@t> bhcpns.org Fri Feb 4 11:02:13 2011 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Fri Feb 4 11:02:25 2011 Subject: [Histonet] CLIA classes? In-Reply-To: <1296816908.91084963@192.168.4.58> Message-ID: Anderson Education offers a good course on this for CEU's. You can contact them at 1-800-532-2332 Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 erika@mapslab.net Sent by: histonet-bounces@lists.utsouthwestern.edu 02/04/2011 04:55 AM To "Sands, Jenn" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] CLIA classes? I would be interested in this as well. I supervise a dermpath lab and have for five years. I have always passed with no deficiencies on my inspections. The inspector always tells me that I document to much stuff. Thanks, Erika -----Original Message----- From: "Sands, Jenn" Sent: Thursday, February 3, 2011 11:02am To: indytreegers@sbcglobal.net, Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA classes? I would be interested in this information as well. Thank you. Jenn Sands, CT(ASCP)cm, HTL(ASCP)cm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of indytreegers@sbcglobal.net Sent: Thursday, February 03, 2011 9:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA classes? Hi all, Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab? Thanks for any advice you can give me on this! Lyn L. Treeger, B.S.HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Kim.Donadio <@t> bhcpns.org Fri Feb 4 11:13:52 2011 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Fri Feb 4 11:15:35 2011 Subject: [Histonet] Her2 Fixation Requirement In-Reply-To: Message-ID: We adhere the ink with bouins sol'n. Have had no problems. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Rathborne, Toni" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/03/2011 04:54 PM To "Weems, Joyce" , "Paula Lucas" , cc Subject RE: [Histonet] Her2 Fixation Requirement Does anyone have issues with inking the specimen after it has been placed in formalin? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Wednesday, February 02, 2011 12:59 PM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Her2 Fixation Requirement We have the nurse document the time of removal on the requisition. The next requisition update will have a spot for this. They have been very cooperative and do a good job. In addition, you must document the "cold ischmic time" - that is the time from removal until time in formalin. This is important when the specimen goes for xray or whatever. So there is a removal time, an into formalin time and an out of formalin time. Then if we don't have time allowed to meet the fixation time with the regular, it is put on a late processor, if we have one available, or it is held overnight. And if it has to come off on Sunday, we have a med tech remove it from the processor and it waits for us to come on Monday to embed it. The pathologists document all this time in the report. Best! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, February 02, 2011 12:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2 Fixation Requirement Hello histoland I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback. Would you mind sharing with me the actions you are taking to comply with the guideline? We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin. I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor. The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out. Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From andrea.conard <@t> gmail.com Fri Feb 4 11:18:11 2011 From: andrea.conard <@t> gmail.com (andrea conard) Date: Fri Feb 4 11:18:15 2011 Subject: [Histonet] CE Message-ID: I'm looking for some cost effective CE for my staff. Does anyone know if the CAP still has TechSamples? Please email at andrea.conard@atlanticare.org From jkiernan <@t> uwo.ca Fri Feb 4 11:42:07 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Feb 4 11:42:11 2011 Subject: [Histonet] thionin staining Message-ID: If this is for Nissl staining, the pH of the thionine solution should be 3.5 to 4 and the following rinses should be no more acidic than that. Distilled water is suitable. Alcohol-water mixtures generally remove dyes to a greater extent than 100% alcohol. I shake the washed slides to get rid of most of the water, then go straight to the first of 3 changes of 100% ethanol. Almost no blue is lost from the sections. The first alcohol isn't 100% any more after one or two batches of slides have gone through, so for large numbers of slides it is more economical to go through 95% (quickly) then 3X100%. If you use 50% and 70% alcohol you can expect dye to be extracted from the stained sections. It's not unusual to get unsatisfactory batches of thionine, and for at least one purpose, showing the canaliculi and lacunae of bone, the late Russ Allison found that proper staining could be obtained only with batches of thionine that had been certified by the Biological Stain Commission. See Allison RT (1995) Picro-thionin (Schmorl) staining of bone and other hard tissues. Brit. J. Biomed. Sci. 52: 162-164. The B.S.C.'s tests for thionine are a mast cell stain and a stain for plant tissue infected with a fungus. The dye must also meet spectrophotometric criteria. See Penney DP (2002) Analysis and testing of biological stains - the Biological Stain commission Procedures. Biotech. Histochem. 77:237-275. A minor revision to the criteria for thionine was published in 2008: Lyon HO & Kiernan JA (2008) Notes from the Biological Stain Commission. Biotech. Histochem. 83(5):285-288. Make sure your dye really is thionine (CI 52000). There is a dye called thionine blue (CI 52025, Basic blue 25) that is not a substitute. See Conn's Biological Stains or Bryan Llewellyn's StainsFile http://stainsfile.info/StainsFile/dyes/52000.htm . Finally, the word thionine is commonly mis-spelled, without its terminal e. The e should be there because thionine is an amine, in contrast to eosin, which is not. Dictionaries (English or US) give the correct spelling. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: An Eerdekens Date: Friday, February 4, 2011 3:21 Subject: [Histonet] thionin staining To: "histonet@lists.utsouthwestern.edu" > Dear all, > > I have problems with thionin staining. I am using this on > paraffin slices of hypothalami. In the past, I never experienced > problems, but the last times, the staining is washed off by > going through the ethanol after staining (50%, 70%, 90%, 100%). > I tried already a few protocols, but every time the same result. > Does anybody have experience with it? > > regards > > An Eerdekens > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Feb 4 12:12:01 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Feb 4 12:12:04 2011 Subject: [Histonet] veterinary IHC for Ornithobacterium Message-ID: <40B81232AD4048BA848C9586ABAA9255@auxs.umn.edu> Does anyone do IHC staining for Ornithobacterium rhinotracheale (ORT), a respiratory pathogen in poultry? I'm trying to find a lab to do it, or a vendor source for antibody. Much thanks in advance, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) From shshaw <@t> WPI.EDU Fri Feb 4 13:41:38 2011 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Fri Feb 4 13:41:45 2011 Subject: [Histonet] HCN4 staining Message-ID: <223F1D19A67B3245ADED1F18858077E005077552@S281.admin.wpi.edu> Hello, I'm hoping somebody out there can give me some help with HCN4 staining, I was asked to stain HCN4 on mouse heart and it needs to stain the SA node. It is very hard to find that area since the node is very small. I have tried serial sections but not sure I hit the area or if I'm having problems with the stain. The antibody is Rat monoclonal Secondary Alexa Flour 488 goat anti-rat Counter stain is Hoechst I tried frozen and paraffin sections with no luck Any suggestions will help greatly Thanks, Sharon From mtitford <@t> aol.com Fri Feb 4 14:06:18 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Feb 4 14:06:29 2011 Subject: [Histonet] Acetylcholinesterase Message-ID: <8CD92BE1E6196A0-1C1C-38BD7@Webmail-d105.sysops.aol.com> Nicole Cosenza asks about acetylcholinesterase methods. Years and years ago in London we used Koelle's method to demonstrate cholinesterase in muscle and mouse diaphragms. The method we followed was in Pearse E, Histochemistry - Theoretical and Applied. Volume 2. Churchill Livingstone. London 1972. Pages 1312 - 1316 has several different methods. The enzyme was visualized with ammonium sulphide. The tissues were mounted in glycerine jelly. If the method worked too well and we could not see the motor end plates, we adjusted the pH to reduce staining. Dr Filipe went on to publish her own method in Filipe I., Lake B., Histochemistry in Pathology. Churchill Livingstone. London 1983 page 322. In her method, she used osmium to visualise the enzyme, instead of ammonium sulphide. Stain technology used to have articles about the method too. I have not heard of Karnovsky or Roots methods, but lot of different methods were published in the early days of enzyme histochemistry. Hope this helps Michael Titford Pathology - USA Mobile AL From nancy <@t> pathologyarts.com Fri Feb 4 14:12:13 2011 From: nancy <@t> pathologyarts.com (Nancy) Date: Fri Feb 4 14:12:02 2011 Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? Message-ID: <003a01cbc4a7$d0054560$700fd020$@com> At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com From LSetlak <@t> childrensmemorial.org Fri Feb 4 14:22:50 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Fri Feb 4 14:22:55 2011 Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? In-Reply-To: <003a01cbc4a7$d0054560$700fd020$@com> References: <003a01cbc4a7$d0054560$700fd020$@com> Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7683A3@CMHEXCC01MBX.childrensmemorial.org> We too have the same Coverslipper but we use the Sakura brand tape. We tried the KP tape but did not like it; it seemed to have problems sticking on the slides and we had a few other issues but I don't remember what they were. Could it be that there needs to be more xylene applied to slides- it may be air and dry tissue that you're seeing. Hope this helps. Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Sent: Friday, February 04, 2011 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Fri Feb 4 14:44:19 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Feb 4 14:44:25 2011 Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? In-Reply-To: <003a01cbc4a7$d0054560$700fd020$@com> References: <003a01cbc4a7$d0054560$700fd020$@com> Message-ID: <57BE698966D5C54EAE8612E8941D76830A699BCD@EXCHANGE3.huntingtonhospital.com> Nancy, We get areas that appear brown when the tape doesn't lay flat or adhere to the slide. Most of the time this happens on decal slides or hard tissue where the tissue may be lifted a little. Try recoverslipping. Also, if it's not happening on these types of tissue, you may need more xylene dispensed. Sometimes, we just have to resort to the old-fashioned way of coverslipping with mounting media. I've never used the Klinipath tape. I prefer to stick with Sakura's brand - don't want to take any chances! Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Sent: Friday, February 04, 2011 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Feb 4 15:15:03 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Feb 4 15:15:06 2011 Subject: [Histonet] Karnovsky and Roots stain Message-ID: Karnovsky & Roots is (IMHO) the best histochemical method for choline esterase activity. In muscles, acetylcholinesterase (AChE) is the only such esterase shown by this method, and it is in the subneural apparatus of the motor endplate. Some counterstains (notably silver methods for the innervating axons) can remove the brown copper ferrocyanide product. Another way to show motor endplates is with a method that picks up all esterases. In muscle, the endplate AChE shows up sooner than the enzymes present in all cells. Indigogenic esterase methods can be followed by silver staining of axons. Why do you need or want to use fresh frozen, unfixed tissue sections? This makes no sense in the world of esterase activity histochemistry. There haven't been any developments in this field since the 1960s other than labelled alpha-bungarotoxin and immunohistochemistry. An inexpensive book is Van Noorden CJF & Frederiks WM 1992. Enzyme Histochemistry. Oxford Univ Press and Royal Microscopical Soc. ISBN0198564341. Another one is Lojda, Gossrau & Schiebler 1979. Enzyme histochemistry. Berlin: Springer. ISBN 0387092692. A quick web search indicates that both are available and cost less than $10 second-hand. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Cosenza Date: Thursday, February 3, 2011 18:45 Subject: [Histonet] Karnovsky and Roots stain To: histonet@lists.utsouthwestern.edu > I am looking into a project involving motor end plate staining. > Literature that I've found continually references Karnovsky and > Roots from the 60s. However the papers are not > supplying all the details. > > Does anyone do AchE staining by this method on fresh frozen, > unfixed tissue sections? If so, can I get a more detailed > protocol (fixation steps, washes, etc)? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Fri Feb 4 15:20:19 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Fri Feb 4 15:20:25 2011 Subject: [Histonet] Karnovsky and Roots stain In-Reply-To: References: Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7683A7@CMHEXCC01MBX.childrensmemorial.org> We do ACH staining on rectal biopsies to evaluate for ganglion cells for Hirschsprung's disease. Our stain is performed on fresh frozen tissue and I believe it's the Karnovsky method, but I'm not sure. Feel free to email if you are interested in out procedure. Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Friday, February 04, 2011 3:15 PM To: Nicole Cosenza Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Karnovsky and Roots stain Karnovsky & Roots is (IMHO) the best histochemical method for choline esterase activity. In muscles, acetylcholinesterase (AChE) is the only such esterase shown by this method, and it is in the subneural apparatus of the motor endplate. Some counterstains (notably silver methods for the innervating axons) can remove the brown copper ferrocyanide product. Another way to show motor endplates is with a method that picks up all esterases. In muscle, the endplate AChE shows up sooner than the enzymes present in all cells. Indigogenic esterase methods can be followed by silver staining of axons. Why do you need or want to use fresh frozen, unfixed tissue sections? This makes no sense in the world of esterase activity histochemistry. There haven't been any developments in this field since the 1960s other than labelled alpha-bungarotoxin and immunohistochemistry. An inexpensive book is Van Noorden CJF & Frederiks WM 1992. Enzyme Histochemistry. Oxford Univ Press and Royal Microscopical Soc. ISBN0198564341. Another one is Lojda, Gossrau & Schiebler 1979. Enzyme histochemistry. Berlin: Springer. ISBN 0387092692. A quick web search indicates that both are available and cost less than $10 second-hand. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Cosenza Date: Thursday, February 3, 2011 18:45 Subject: [Histonet] Karnovsky and Roots stain To: histonet@lists.utsouthwestern.edu > I am looking into a project involving motor end plate staining. > Literature that I've found continually references Karnovsky and > Roots from the 60s. However the papers are not > supplying all the details. > > Does anyone do AchE staining by this method on fresh frozen, > unfixed tissue sections? If so, can I get a more detailed > protocol (fixation steps, washes, etc)? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ncosenza <@t> siumed.edu Fri Feb 4 15:53:49 2011 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Fri Feb 4 15:53:53 2011 Subject: [Histonet] combined cholinesterase-silver stain Message-ID: <4D4C756D.4000404@siumed.edu> I am looking into staining motor end plates. I've come across this combined cholinesterase-silver stain (reference Pestronk and Drachman, 1978). Based on the date of the paper, I'm wondering what the current technique is for this double staining. Anyone currently doing AchE and axon staining on fresh frozen muscle sections? From akemiat3377 <@t> yahoo.com Fri Feb 4 16:04:56 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Feb 4 16:05:00 2011 Subject: [Histonet] pH Meter Message-ID: <506187.89374.qm@web113811.mail.gq1.yahoo.com> Happy Friday everyone! I am in the market for a new pH Meter, and was wondering if any of you had any preferences.? I want to keep it simple as possible for?the staff.? Also, I ?don't want to spend a ton of money. One of our fellow histonet subscribers?recommended purchasing a pH Meter that did a? 2-step calibration verses a 3-step.? I've always used a 3-step calibration.? Any thoughts.? ? I realize that it must have a good glass electrode, calibrated daily, prior to use, and all the maintenence must be followed to keep it in good working order. ? If you have any suggestions, please provide the make and model #, and if you have a vendor who provides it such as Fisher or Thermo, that would be an added bonus. Thank you, and have a great weekend! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From ruppert.amysue <@t> marshfieldclinic.org Fri Feb 4 18:03:05 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Fri Feb 4 18:03:12 2011 Subject: [Histonet] Amylase Digestion for glycogen Message-ID: <201102050003.p15030nh007461@spamfilt> Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From jkiernan <@t> uwo.ca Fri Feb 4 23:11:35 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Feb 4 23:11:41 2011 Subject: [Histonet] Amylase Digestion for glycogen Message-ID: <7410bdbf6866.4d4c95b7@uwo.ca>
Hello,
?We are looking to switch f diastase digestion for glycogen to Amylase digestion. I have new protocol worked up, but one of the Pathologists I work with wo uld like to have an idea of how many labs out there are using Amylase in you use who you are appreciated.

Marshfield Labs
____________ _______________________ 5F _______________________ 5F message may information.? If you destroy the e-mail message are prohibited from retaining, any information contained within. advise of the erroneous delivery by re Thank you for your cooperation.
From pknutsen <@t> physics.ucsd.edu Sat Feb 5 01:22:41 2011 From: pknutsen <@t> physics.ucsd.edu (Per Magne Knutsen) Date: Sat Feb 5 01:22:49 2011 Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be obtained? Message-ID: Hi, I've searched every catalog and website for "Fast Blue" (Diamidino compound 253/50), widely used for neuronal retrograde tracing. One recent paper using this compound is: Porreroa et al "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice", Brain Research, 1345, 59-72 where the authors as many before them cite "Dr. Illing GmbH & Co. KG, Gro?-Umstadt, Germany" as the origin. I have been unable to locate this particular vendor. I've written the authors and am waiting for an answer. In the meanwhile, can anyone on this list help? Kindly, *Per M Knutsen *Department of Physics University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0374 T: +1 858 405 2868 E: pknutsen@ucsd.edu W: http://pmknutsen.blogspot.com * * From koellingr <@t> comcast.net Sat Feb 5 09:20:01 2011 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Feb 5 09:25:05 2011 Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be obtained? In-Reply-To: Message-ID: <666683375.5867.1296919201956.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Hello Per, Have no particular interest in neuronal retrograde tracing and have certainly never used anything for that. Just general scientific curiosity and a few minutes time awaiting a home project.? Found Fast Blue (synonym: Diamidino compound 253/50) on Sigma website cat # F5756. Good luck. Ray Ray Koelling PhenoPath Labs Seattle WA ----- Original Message ----- From: "Per Magne Knutsen" To: histonet@lists.utsouthwestern.edu Sent: Friday, February 4, 2011 11:22:41 PM Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be????????obtained? Hi, I've searched every catalog and website for "Fast Blue" (Diamidino compound 253/50), widely used for neuronal retrograde tracing. One recent paper using this compound is: Porreroa et al "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice", Brain Research, 1345, 59-72 where the authors as many before them cite "Dr. Illing GmbH & Co. KG, Gro?-Umstadt, Germany" as the origin. I have been unable to locate this particular vendor. I've written the authors and am waiting for an answer. In the meanwhile, can anyone on this list help? Kindly, *Per M Knutsen *Department of Physics University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0374 T: +1 858 405 2868 E: pknutsen@ucsd.edu W: http://pmknutsen.blogspot.com * * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Feb 5 10:42:57 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 5 10:43:05 2011 Subject: [Histonet] Amylase Digestion for glycogen In-Reply-To: <201102050003.p15030nh007461@spamfilt> Message-ID: <228486.12081.qm@web65712.mail.ac4.yahoo.com> You are dealing with synonyms of the same enzyme but the "amylase" protocol always is slightly more complex. Stick with "diastase" and, please, never use your own saliva for this procedure!!!! Ren? J. --- On Fri, 2/4/11, Ruppert, Amysue wrote: From: Ruppert, Amysue Subject: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 4, 2011, 7:03 PM Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information.? If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.? Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.? Thank you for your cooperation. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Feb 5 11:25:21 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Feb 5 11:26:00 2011 Subject: SPAM-LOW: [Histonet] CE In-Reply-To: References: Message-ID: <7B33835B42264FAE93FD4F474B9283CE@prueggihctechlt> It was ASCP that had TechSample, they do not have that anymore. The teleconferences from NSH are reasonable and can be done at a distance. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of andrea conard Sent: Friday, February 04, 2011 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] CE I'm looking for some cost effective CE for my staff. Does anyone know if the CAP still has TechSamples? Please email at andrea.conard@atlanticare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Feb 5 12:58:16 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Feb 5 12:59:02 2011 Subject: SPAM-LOW: [Histonet] CE In-Reply-To: <7B33835B42264FAE93FD4F474B9283CE@prueggihctechlt> References: <7B33835B42264FAE93FD4F474B9283CE@prueggihctechlt> Message-ID: <9A720D7CE19C493EB6B55A4A5BA495F3@prueggihctechlt> Also I just thought of the exercises in JOH, it think they are giving some CEU credits for them, plus the traveling seminars given by NSH the rest of the year apart for the NSH S/C, see if any are in your area. The IHC Forum is going to be in the Denver area in July this year, July 16 I believe, Denver is pretty centrally located in the country. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Saturday, February 05, 2011 10:25 AM To: 'andrea conard'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] CE It was ASCP that had TechSample, they do not have that anymore. The teleconferences from NSH are reasonable and can be done at a distance. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of andrea conard Sent: Friday, February 04, 2011 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] CE I'm looking for some cost effective CE for my staff. Does anyone know if the CAP still has TechSamples? Please email at andrea.conard@atlanticare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Feb 5 14:04:55 2011 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Feb 5 14:05:49 2011 Subject: [Histonet] RE:Acetylcholinesterase In-Reply-To: <7bd2a563-3ddd-472d-bdfe-75194e123643@KCL-ETS01.ds.kcl.ac.uk> References: <7bd2a563-3ddd-472d-bdfe-75194e123643@KCL-ETS01.ds.kcl.ac.uk> Message-ID: <11D9615B89C10747B1C985966A63D7CA33B3D026DF@KCL-MAIL04.kclad.ds.kcl.ac.uk> Hey! The GREAT Brian Lake was THE Man behind it all, imho. Respect to Him. Filipe, jest like Hiederman/Heyderman?, became "famous" because of the people/technicians who did all of the work and...never got the credit ;-) Carl Hobbs Histology Manager Wolfson CARD Kings College London 020 78486813 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 05 February 2011 18:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. veterinary IHC for Ornithobacterium (Jan Shivers) 2. HCN4 staining (Shaw, Sharon) 3. Acetylcholinesterase (mtitford@aol.com) 4. Artifact or dirt on slides using a tape cover slipper? (Nancy) 5. RE: Artifact or dirt on slides using a tape cover slipper? (Setlak, Lisa) 6. RE: Artifact or dirt on slides using a tape cover slipper? (Laurie Colbert) 7. Re: Karnovsky and Roots stain (John Kiernan) 8. RE: Karnovsky and Roots stain (Setlak, Lisa) 9. combined cholinesterase-silver stain (Nicole Cosenza) 10. pH Meter (Akemi Allison) 11. Amylase Digestion for glycogen (Ruppert, Amysue) 12. Re: Amylase Digestion for glycogen (John Kiernan) 13. Where can "Fast Blue" (Diamidino compound 253/50) be obtained? (Per Magne Knutsen) 14. Re: Where can "Fast Blue" (Diamidino compound 253/50) be obtained? (koellingr@comcast.net) 15. Re: Amylase Digestion for glycogen (Rene J Buesa) 16. RE: SPAM-LOW: [Histonet] CE (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Fri, 4 Feb 2011 12:12:01 -0600 From: "Jan Shivers" Subject: [Histonet] veterinary IHC for Ornithobacterium To: "histonet" Message-ID: <40B81232AD4048BA848C9586ABAA9255@auxs.umn.edu> Content-Type: text/plain; charset="iso-8859-1" Does anyone do IHC staining for Ornithobacterium rhinotracheale (ORT), a respiratory pathogen in poultry? I'm trying to find a lab to do it, or a vendor source for antibody. Much thanks in advance, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ------------------------------ Message: 2 Date: Fri, 04 Feb 2011 14:41:38 -0500 From: "Shaw, Sharon" Subject: [Histonet] HCN4 staining To: "histonet@lists.utsouthwestern.edu" Message-ID: <223F1D19A67B3245ADED1F18858077E005077552@S281.admin.wpi.edu> Content-Type: text/plain; charset="us-ascii" Hello, I'm hoping somebody out there can give me some help with HCN4 staining, I was asked to stain HCN4 on mouse heart and it needs to stain the SA node. It is very hard to find that area since the node is very small. I have tried serial sections but not sure I hit the area or if I'm having problems with the stain. The antibody is Rat monoclonal Secondary Alexa Flour 488 goat anti-rat Counter stain is Hoechst I tried frozen and paraffin sections with no luck Any suggestions will help greatly Thanks, Sharon ------------------------------ Message: 3 Date: Fri, 04 Feb 2011 15:06:18 -0500 From: mtitford@aol.com Subject: [Histonet] Acetylcholinesterase To: histonet@lists.utsouthwestern.edu Message-ID: <8CD92BE1E6196A0-1C1C-38BD7@Webmail-d105.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Nicole Cosenza asks about acetylcholinesterase methods. Years and years ago in London we used Koelle's method to demonstrate cholinesterase in muscle and mouse diaphragms. The method we followed was in Pearse E, Histochemistry - Theoretical and Applied. Volume 2. Churchill Livingstone. London 1972. Pages 1312 - 1316 has several different methods. The enzyme was visualized with ammonium sulphide. The tissues were mounted in glycerine jelly. If the method worked too well and we could not see the motor end plates, we adjusted the pH to reduce staining. Dr Filipe went on to publish her own method in Filipe I., Lake B., Histochemistry in Pathology. Churchill Livingstone. London 1983 page 322. In her method, she used osmium to visualise the enzyme, instead of ammonium sulphide. Stain technology used to have articles about the method too. I have not heard of Karnovsky or Roots methods, but lot of different methods were published in the early days of enzyme histochemistry. Hope this helps Michael Titford Pathology - USA Mobile AL ------------------------------ Message: 4 Date: Fri, 4 Feb 2011 12:12:13 -0800 From: "Nancy" Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? To: Message-ID: <003a01cbc4a7$d0054560$700fd020$@com> Content-Type: text/plain; charset="us-ascii" At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com ------------------------------ Message: 5 Date: Fri, 4 Feb 2011 14:22:50 -0600 From: "Setlak, Lisa" Subject: RE: [Histonet] Artifact or dirt on slides using a tape cover slipper? To: 'Nancy' , "histonet@lists.utsouthwestern.edu" Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7683A3@CMHEXCC01MBX.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" We too have the same Coverslipper but we use the Sakura brand tape. We tried the KP tape but did not like it; it seemed to have problems sticking on the slides and we had a few other issues but I don't remember what they were. Could it be that there needs to be more xylene applied to slides- it may be air and dry tissue that you're seeing. Hope this helps. Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Sent: Friday, February 04, 2011 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 4 Feb 2011 12:44:19 -0800 From: "Laurie Colbert" Subject: RE: [Histonet] Artifact or dirt on slides using a tape cover slipper? To: "Nancy" , Message-ID: <57BE698966D5C54EAE8612E8941D76830A699BCD@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Nancy, We get areas that appear brown when the tape doesn't lay flat or adhere to the slide. Most of the time this happens on decal slides or hard tissue where the tissue may be lifted a little. Try recoverslipping. Also, if it's not happening on these types of tissue, you may need more xylene dispensed. Sometimes, we just have to resort to the old-fashioned way of coverslipping with mounting media. I've never used the Klinipath tape. I prefer to stick with Sakura's brand - don't want to take any chances! Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Sent: Friday, February 04, 2011 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 04 Feb 2011 16:15:03 -0500 From: John Kiernan Subject: Re: [Histonet] Karnovsky and Roots stain To: Nicole Cosenza Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Karnovsky & Roots is (IMHO) the best histochemical method for choline esterase activity. In muscles, acetylcholinesterase (AChE) is the only such esterase shown by this method, and it is in the subneural apparatus of the motor endplate. Some counterstains (notably silver methods for the innervating axons) can remove the brown copper ferrocyanide product. Another way to show motor endplates is with a method that picks up all esterases. In muscle, the endplate AChE shows up sooner than the enzymes present in all cells. Indigogenic esterase methods can be followed by silver staining of axons. Why do you need or want to use fresh frozen, unfixed tissue sections? This makes no sense in the world of esterase activity histochemistry. There haven't been any developments in this field since the 1960s other than labelled alpha-bungarotoxin and immunohistochemistry. An inexpensive book is Van Noorden CJF & Frederiks WM 1992. Enzyme Histochemistry. Oxford Univ Press and Royal Microscopical Soc. ISBN0198564341. Another one is Lojda, Gossrau & Schiebler 1979. Enzyme histochemistry. Berlin: Springer. ISBN 0387092692. A quick web search indicates that both are available and cost less than $10 second-hand. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Cosenza Date: Thursday, February 3, 2011 18:45 Subject: [Histonet] Karnovsky and Roots stain To: histonet@lists.utsouthwestern.edu > I am looking into a project involving motor end plate staining. > Literature that I've found continually references Karnovsky and > Roots from the 60s. However the papers are not > supplying all the details. > > Does anyone do AchE staining by this method on fresh frozen, unfixed > tissue sections? If so, can I get a more detailed protocol (fixation > steps, washes, etc)? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 4 Feb 2011 15:20:19 -0600 From: "Setlak, Lisa" Subject: RE: [Histonet] Karnovsky and Roots stain To: 'John Kiernan' , Nicole Cosenza Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7683A7@CMHEXCC01MBX.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" We do ACH staining on rectal biopsies to evaluate for ganglion cells for Hirschsprung's disease. Our stain is performed on fresh frozen tissue and I believe it's the Karnovsky method, but I'm not sure. Feel free to email if you are interested in out procedure. Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Friday, February 04, 2011 3:15 PM To: Nicole Cosenza Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Karnovsky and Roots stain Karnovsky & Roots is (IMHO) the best histochemical method for choline esterase activity. In muscles, acetylcholinesterase (AChE) is the only such esterase shown by this method, and it is in the subneural apparatus of the motor endplate. Some counterstains (notably silver methods for the innervating axons) can remove the brown copper ferrocyanide product. Another way to show motor endplates is with a method that picks up all esterases. In muscle, the endplate AChE shows up sooner than the enzymes present in all cells. Indigogenic esterase methods can be followed by silver staining of axons. Why do you need or want to use fresh frozen, unfixed tissue sections? This makes no sense in the world of esterase activity histochemistry. There haven't been any developments in this field since the 1960s other than labelled alpha-bungarotoxin and immunohistochemistry. An inexpensive book is Van Noorden CJF & Frederiks WM 1992. Enzyme Histochemistry. Oxford Univ Press and Royal Microscopical Soc. ISBN0198564341. Another one is Lojda, Gossrau & Schiebler 1979. Enzyme histochemistry. Berlin: Springer. ISBN 0387092692. A quick web search indicates that both are available and cost less than $10 second-hand. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Cosenza Date: Thursday, February 3, 2011 18:45 Subject: [Histonet] Karnovsky and Roots stain To: histonet@lists.utsouthwestern.edu > I am looking into a project involving motor end plate staining. > Literature that I've found continually references Karnovsky and > Roots from the 60s. However the papers are not > supplying all the details. > > Does anyone do AchE staining by this method on fresh frozen, unfixed > tissue sections? If so, can I get a more detailed protocol (fixation > steps, washes, etc)? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 04 Feb 2011 15:53:49 -0600 From: Nicole Cosenza Subject: [Histonet] combined cholinesterase-silver stain To: histonet@lists.utsouthwestern.edu Message-ID: <4D4C756D.4000404@siumed.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I am looking into staining motor end plates. I've come across this combined cholinesterase-silver stain (reference Pestronk and Drachman, 1978). Based on the date of the paper, I'm wondering what the current technique is for this double staining. Anyone currently doing AchE and axon staining on fresh frozen muscle sections? ------------------------------ Message: 10 Date: Fri, 4 Feb 2011 14:04:56 -0800 (PST) From: Akemi Allison Subject: [Histonet] pH Meter To: histonet Message-ID: <506187.89374.qm@web113811.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Happy Friday everyone! I am in the market for a new pH Meter, and was wondering if any of you had any preferences.? I want to keep it simple as possible for?the staff.? Also, I ?don't want to spend a ton of money. One of our fellow histonet subscribers?recommended purchasing a pH Meter that did a? 2-step calibration verses a 3-step.? I've always used a 3-step calibration.? Any thoughts.? ? I realize that it must have a good glass electrode, calibrated daily, prior to use, and all the maintenence must be followed to keep it in good working order. ? If you have any suggestions, please provide the make and model #, and if you have a vendor who provides it such as Fisher or Thermo, that would be an added bonus. Thank you, and have a great weekend! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 11 Date: Fri, 04 Feb 2011 18:03:05 -0600 From: "Ruppert, Amysue" Subject: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" Message-ID: <201102050003.p15030nh007461@spamfilt> Content-Type: text/plain; charset="iso-8859-1" Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ------------------------------ Message: 12 Date: Sat, 05 Feb 2011 00:11:35 -0500 From: John Kiernan Subject: Re: [Histonet] Amylase Digestion for glycogen To: "Ruppert, Amysue" , "histonet@lists.utsouthwestern.edu" Message-ID: <7410bdbf6866.4d4c95b7@uwo.ca> Content-Type: text/plain; charset="iso-8859-1"
Hello,
?We are looking to switch f diastase digestion for glycogen to Amylase digestion. I have new protocol worked up, but one of the Pathologists I work with wo uld like to have an idea of how many labs out there are using Amylase in you use who you are appreciated.

Marshfield Labs
____________ _______________________ 5F _______________________ 5F message may information.? If you destroy the e-mail message are prohibited from retaining, any information contained within. advise of the erroneous delivery by re Thank you for your cooperation.
------------------------------ Message: 13 Date: Fri, 4 Feb 2011 23:22:41 -0800 From: Per Magne Knutsen Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be obtained? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi, I've searched every catalog and website for "Fast Blue" (Diamidino compound 253/50), widely used for neuronal retrograde tracing. One recent paper using this compound is: Porreroa et al "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice", Brain Research, 1345, 59-72 where the authors as many before them cite "Dr. Illing GmbH & Co. KG, Gro??-Umstadt, Germany" as the origin. I have been unable to locate this particular vendor. I've written the authors and am waiting for an answer. In the meanwhile, can anyone on this list help? Kindly, *Per M Knutsen *Department of Physics University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0374 T: +1 858 405 2868 E: pknutsen@ucsd.edu W: http://pmknutsen.blogspot.com * * ------------------------------ Message: 14 Date: Sat, 5 Feb 2011 15:20:01 +0000 (UTC) From: koellingr@comcast.net Subject: Re: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be obtained? To: Per Magne Knutsen Cc: histonet@lists.utsouthwestern.edu Message-ID: <666683375.5867.1296919201956.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Hello Per, Have no particular interest in neuronal retrograde tracing and have certainly never used anything for that. Just general scientific curiosity and a few minutes time awaiting a home project.?? Found Fast Blue (synonym: Diamidino compound 253/50) on Sigma website cat # F5756. Good luck. Ray Ray Koelling PhenoPath Labs Seattle WA ----- Original Message ----- From: "Per Magne Knutsen" To: histonet@lists.utsouthwestern.edu Sent: Friday, February 4, 2011 11:22:41 PM Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be????????????????obtained? Hi, I've searched every catalog and website for "Fast Blue" (Diamidino compound 253/50), widely used for neuronal retrograde tracing. One recent paper using this compound is: Porreroa et al "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice", Brain Research, 1345, 59-72 where the authors as many before them cite "Dr. Illing GmbH & Co. KG, Gro??-Umstadt, Germany" as the origin. I have been unable to locate this particular vendor. I've written the authors and am waiting for an answer. In the meanwhile, can anyone on this list help? Kindly, *Per M Knutsen *Department of Physics University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0374 T: +1 858 405 2868 E: pknutsen@ucsd.edu W: http://pmknutsen.blogspot.com * * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Sat, 5 Feb 2011 08:42:57 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" , AmysueRuppert Message-ID: <228486.12081.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You are dealing with synonyms of the same enzyme but the "amylase" protocol always is slightly more complex. Stick with "diastase" and, please, never use your own saliva for this procedure!!!! Ren? J. --- On Fri, 2/4/11, Ruppert, Amysue wrote: From: Ruppert, Amysue Subject: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 4, 2011, 7:03 PM Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information.? If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.? Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.? Thank you for your cooperation. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Sat, 5 Feb 2011 10:25:21 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] CE To: "'andrea conard'" , Message-ID: <7B33835B42264FAE93FD4F474B9283CE@prueggihctechlt> Content-Type: text/plain; charset="US-ASCII" It was ASCP that had TechSample, they do not have that anymore. The teleconferences from NSH are reasonable and can be done at a distance. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of andrea conard Sent: Friday, February 04, 2011 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] CE I'm looking for some cost effective CE for my staff. Does anyone know if the CAP still has TechSamples? Please email at andrea.conard@atlanticare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 87, Issue 10 **************************************** From rllott <@t> bellsouth.net Sat Feb 5 14:55:00 2011 From: rllott <@t> bellsouth.net (Robert L. Lott) Date: Sat Feb 5 14:55:14 2011 Subject: [Histonet] TTF-1 Staining Message-ID: Subject: TTF-1 Staining For those of you who participate in the CAP/NSH HistoQIP program... and did so in back in 2009, one of the IHC challenges that year was TTF-1. >From the critique published with that particular challenge, enrolled participants may have read with interest the following 2 excerpts: (I have included the references) The majority of the published studies on the immunoreactivity of TTF-1 have been conducted using two commercially available clones, 8G7G3/1 and SPT24. It has been shown that SPT24 is more sensitive than 8G7G3/1 for the detection of pulmonary adenocarcinomas. In a comparative study of 86 primary pulmonary adenocarcinomas by Comperat et al., nuclear staining was detected in 72 cases (84%) with SPT24 and 56 cases (65%) with 8G7G3/1.6 The clone 8G7G3/1 cross reacts with cytoplasmic mitochondrial proteins in liver cells while the clone SPT24 does not. Pathologists have taken advantage of this phenomenon and used it to study carcinomas of the liver. In a recent study, cytoplasmic reactivity for TTF-1 was observed in 71% of hepatocellular carcinomas with the 8G7G3/1 antibody and was more sensitive than Hep-Par 1. The cytoplasmic reactivity is seen even when using biotin-free protocols and seems fairly specific for hepatocellular carcinoma.11 6. Comperat E, Zhang F, Perrotin C, Molina T, Magdeleinat P, Marmey B, Regnard JF, Audouin J, Camilleri-Broet S. Variable sensitivity and specificity of TTF1 antibodies in lung metastatic adenocarcinoma of colorectal origin. Mod Pathol. 2005; 18(10):1371-1376. 11. Pang Y, von Turkovich M, Wu H, Mitchell J, Mount S, Taatjes D, Cooper K. The binding of thyroid transcription factor-1 and hepatocyte paraffin 1 to mitochondrial proteins in hepatocytes: a molecular and immunoelectron microscopic study. Am J Clin Pathol. 2006;125(5):722-726. Robert L. Lott, HTL(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, February 03, 2011 2:38 PM To: Subject: [Histonet] TTF-1/background staining How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me. To give you a little background info: We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell Marque. We were having issues with this marker from Lab Vision, so we switched to TTF-1 a while ago. We use the UltraVision LP detection kit from Lab Vision. It's a polymer driven detection kit. The antibody is a ready to use, and we have the time set at 30 minutes. We were getting the background staining on a liver specimen last week, and we also had this problem today on a lung case. My doctor is getting frustrated, and wants me to do something about this, and to repeat the TTF-1 stain on the lung, so if someone can give me some suggestions to try, I'm ready to try them. Thanks in advance, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA From lpwenk <@t> sbcglobal.net Sat Feb 5 16:18:05 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Feb 5 16:18:07 2011 Subject: [Histonet] Teleconference CE In-Reply-To: <7B33835B42264FAE93FD4F474B9283CE@prueggihctechlt> References: <7B33835B42264FAE93FD4F474B9283CE@prueggihctechlt> Message-ID: (Disclaimer to start - I'm the NSH Teleconference coordinator. But I don't get paid for this position. I volunteer to do this, as I believe in continuing education for all histotechs.) Thought I'd expand a little about the NSH teleconfereces. 4th Wed. of each month (Nov and Dec. might be 3rd Wed, depends on date), 1-2 pm Eastern time. $125/teleconference. Your lab can have as many people attend for that price - histotechs, lab assistants, cytotechs, med techs, we've even had some of our transcriptionists attend, if the topic is of interest to them. Everyone attending earns 1 hour CE. About 2 months after the teleconference, your lab gets a CD with the PowerPoint, additional handouts, and the speaker's voice synced to the PowerPoint. Anyone in your lab listening to the CD, up to 2 years after the date of the teleconference, can earn 1 hour CE, when they complete the test that is also on the CD, which is 4 multiple choice questions, which they then either mail in or fax into the NSH Office. This is great for those people who, during the teleconference, had to stay behind to run the lab, work off-shifts, were on vacation or medical leave, or were hired 2 months later. (I also keep the CD's, and use them for training people years later. They just don't earn CE from NSH, but the CD's are still good for training.) So, that $125 per teleconference is really inexpensive, when you divide it by the number of people who can attend. The NSH website for Teleconferences is: http://www.nsh.org/content/nsh-teleconference-series If that doesn't work, go to: www.nsh.org Click on Professional Development Click on Continuing Education Click on Teleconferences The teleconference topics for the remainder of the 2011 year are: Feb. 23 - Nuclear Stains Mar. 23 - Digital Pathology Apr. 27 - Optimizing Antibodies May 25 - Macrosections June 22 - Monoclonal Antibodies July 27 - Cell Cycle and Cancer Aug. 24 - ER and PR Standardization Sept. 28 - Lower GI Biopsies Oct. 26 - Biobank Nov. 16 - Violence in the Workplace Dec. 21 - Chemical Storage Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Patsy Ruegg" Sent: Saturday, February 05, 2011 12:25 PM To: "'andrea conard'" ; Subject: RE: SPAM-LOW: [Histonet] CE > It was ASCP that had TechSample, they do not have that anymore. The > teleconferences from NSH are reasonable and can be done at a distance. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of andrea > conard > Sent: Friday, February 04, 2011 10:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: [Histonet] CE > > I'm looking for some cost effective CE for my staff. Does anyone know if > the > CAP still has TechSamples? > Please email at andrea.conard@atlanticare.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson6848 <@t> yahoo.com Sat Feb 5 17:41:20 2011 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Sat Feb 5 17:41:23 2011 Subject: [Histonet] Looking for position in Texas Message-ID: <74193.39874.qm@web120910.mail.ne1.yahoo.com> ??? Hello all, ???????????????? I am looking for a new histotech position in texas, especially in the houston area?so as to be, ?very?close to my family. Though, any part of texas is okay. ??? I am an HT(ASCP) certified histotech. I do have substancial years of experience and with QIHC(ASCP) ???????? Any leads will be greatly appreciated. ??? Wilson. From njblademaster <@t> gmail.com Sat Feb 5 20:31:32 2011 From: njblademaster <@t> gmail.com (Nathan Jentsch) Date: Sat Feb 5 20:31:56 2011 Subject: [Histonet] Re: Amylase Digestion for glycogen Message-ID: We use amylase digestion for our daily PAS/D stains. The procedure is not that complicated. We preheat the enzyme in a 45 degree C water bath for 10 minutes. Then we let the slides digest with the solution still in the water bath for 10 minutes. Then we rinse in running tap water for 5 minutes and proceed to the periodic acid. Nathan Jentsch BS HT(ASCP) From AnthonyH <@t> chw.edu.au Mon Feb 7 00:40:01 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Feb 7 00:40:13 2011 Subject: [Histonet] Amylase Digestion for glycogen In-Reply-To: <201102050003.p15030nh007461@spamfilt> References: <201102050003.p15030nh007461@spamfilt> Message-ID: <6D6BD1DE8A5571489398B392A38A715706250A@xmdb02.nch.kids> Amysue, Our amylase digestion procedure is quite simple (see: Mangan, V-M, Farago, V., Kelly, M., Henwood, A.F., (2002) "An Amylase Reagent with a Long Shelf Life for the removal of Glycogen from Tissue Sections" J. Histotechnol 25:153.) See below for the method: Amylase Reagent Warning: Harmful, contains azide - see MSDS Alpha Amylase from Bacillus Subtilis (Fluka Cat No 10070,) 1g Oxoid PBS Tablets (Cat No BR14a) 1 tablet Distilled water 100ml Sodium Azide 0.1g This solution, once prepared is stored at 4oC when not in use. A recycled antibody dropper bottle (often used in commercial immunoperoxidase kits) is useful for storage and application. Procedure: 1. Dewax and hydrate paraffin sections, hydrate frozen sections. 2. For amylase digestion, place slides on a rack, cover sections with amylase solution and allow to incubate for 10 minutes at room temperature. 3. Wash slides well in water. 4. Place slides in 1% periodic acid 10 minutes. 5. Wash slides well in water. 6. Rinse slides in distilled water. 7. Place in Schiff's reagent 10 minutes. 8. Rinse slides in distilled water and then wash slides in tap water 3 minutes. 9. Counterstain slides with haematoxylin, differentiate and blue. 10. Dehydrate, clear and mount. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ruppert, Amysue Sent: Saturday, 5 February 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amylase Digestion for glycogen Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From raghulj <@t> orchidpharma.com Mon Feb 7 01:30:46 2011 From: raghulj <@t> orchidpharma.com (raghulj@orchidpharma.com) Date: Mon Feb 7 01:32:08 2011 Subject: [Histonet] pale stained areas H&E In-Reply-To: <036ea81c-42d7-4dde-ba95-b1314950b166@TX2EHSMHS012.ehs.local> References: <036ea81c-42d7-4dde-ba95-b1314950b166@TX2EHSMHS012.ehs.local> Message-ID: Dear histonetters, I have pasted two images img001 and img002 dt. 7feb2011 on staining. The images are of mouse kidney 4micron paraffin embedded formalin fixed sections where the hematoxylin stained areas are pale and not uniform. Is it because of over heating or sections getting dried during coverslipping? Kindly help with your suggestions Regards raghul -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, February 05, 2011 11:32 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. veterinary IHC for Ornithobacterium (Jan Shivers) 2. HCN4 staining (Shaw, Sharon) 3. Acetylcholinesterase (mtitford@aol.com) 4. Artifact or dirt on slides using a tape cover slipper? (Nancy) 5. RE: Artifact or dirt on slides using a tape cover slipper? (Setlak, Lisa) 6. RE: Artifact or dirt on slides using a tape cover slipper? (Laurie Colbert) 7. Re: Karnovsky and Roots stain (John Kiernan) 8. RE: Karnovsky and Roots stain (Setlak, Lisa) 9. combined cholinesterase-silver stain (Nicole Cosenza) 10. pH Meter (Akemi Allison) 11. Amylase Digestion for glycogen (Ruppert, Amysue) 12. Re: Amylase Digestion for glycogen (John Kiernan) 13. Where can "Fast Blue" (Diamidino compound 253/50) be obtained? (Per Magne Knutsen) 14. Re: Where can "Fast Blue" (Diamidino compound 253/50) be obtained? (koellingr@comcast.net) 15. Re: Amylase Digestion for glycogen (Rene J Buesa) 16. RE: SPAM-LOW: [Histonet] CE (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Fri, 4 Feb 2011 12:12:01 -0600 From: "Jan Shivers" Subject: [Histonet] veterinary IHC for Ornithobacterium To: "histonet" Message-ID: <40B81232AD4048BA848C9586ABAA9255@auxs.umn.edu> Content-Type: text/plain; charset="iso-8859-1" Does anyone do IHC staining for Ornithobacterium rhinotracheale (ORT), a respiratory pathogen in poultry? I'm trying to find a lab to do it, or a vendor source for antibody. Much thanks in advance, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ------------------------------ Message: 2 Date: Fri, 04 Feb 2011 14:41:38 -0500 From: "Shaw, Sharon" Subject: [Histonet] HCN4 staining To: "histonet@lists.utsouthwestern.edu" Message-ID: <223F1D19A67B3245ADED1F18858077E005077552@S281.admin.wpi.edu> Content-Type: text/plain; charset="us-ascii" Hello, I'm hoping somebody out there can give me some help with HCN4 staining, I was asked to stain HCN4 on mouse heart and it needs to stain the SA node. It is very hard to find that area since the node is very small. I have tried serial sections but not sure I hit the area or if I'm having problems with the stain. The antibody is Rat monoclonal Secondary Alexa Flour 488 goat anti-rat Counter stain is Hoechst I tried frozen and paraffin sections with no luck Any suggestions will help greatly Thanks, Sharon ------------------------------ Message: 3 Date: Fri, 04 Feb 2011 15:06:18 -0500 From: mtitford@aol.com Subject: [Histonet] Acetylcholinesterase To: histonet@lists.utsouthwestern.edu Message-ID: <8CD92BE1E6196A0-1C1C-38BD7@Webmail-d105.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Nicole Cosenza asks about acetylcholinesterase methods. Years and years ago in London we used Koelle's method to demonstrate cholinesterase in muscle and mouse diaphragms. The method we followed was in Pearse E, Histochemistry - Theoretical and Applied. Volume 2. Churchill Livingstone. London 1972. Pages 1312 - 1316 has several different methods. The enzyme was visualized with ammonium sulphide. The tissues were mounted in glycerine jelly. If the method worked too well and we could not see the motor end plates, we adjusted the pH to reduce staining. Dr Filipe went on to publish her own method in Filipe I., Lake B., Histochemistry in Pathology. Churchill Livingstone. London 1983 page 322. In her method, she used osmium to visualise the enzyme, instead of ammonium sulphide. Stain technology used to have articles about the method too. I have not heard of Karnovsky or Roots methods, but lot of different methods were published in the early days of enzyme histochemistry. Hope this helps Michael Titford Pathology - USA Mobile AL ------------------------------ Message: 4 Date: Fri, 4 Feb 2011 12:12:13 -0800 From: "Nancy" Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? To: Message-ID: <003a01cbc4a7$d0054560$700fd020$@com> Content-Type: text/plain; charset="us-ascii" At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com ------------------------------ Message: 5 Date: Fri, 4 Feb 2011 14:22:50 -0600 From: "Setlak, Lisa" Subject: RE: [Histonet] Artifact or dirt on slides using a tape cover slipper? To: 'Nancy' , "histonet@lists.utsouthwestern.edu" Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7683A3@CMHEXCC01MBX.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" We too have the same Coverslipper but we use the Sakura brand tape. We tried the KP tape but did not like it; it seemed to have problems sticking on the slides and we had a few other issues but I don't remember what they were. Could it be that there needs to be more xylene applied to slides- it may be air and dry tissue that you're seeing. Hope this helps. Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Sent: Friday, February 04, 2011 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 4 Feb 2011 12:44:19 -0800 From: "Laurie Colbert" Subject: RE: [Histonet] Artifact or dirt on slides using a tape cover slipper? To: "Nancy" , Message-ID: <57BE698966D5C54EAE8612E8941D76830A699BCD@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Nancy, We get areas that appear brown when the tape doesn't lay flat or adhere to the slide. Most of the time this happens on decal slides or hard tissue where the tissue may be lifted a little. Try recoverslipping. Also, if it's not happening on these types of tissue, you may need more xylene dispensed. Sometimes, we just have to resort to the old-fashioned way of coverslipping with mounting media. I've never used the Klinipath tape. I prefer to stick with Sakura's brand - don't want to take any chances! Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Sent: Friday, February 04, 2011 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 04 Feb 2011 16:15:03 -0500 From: John Kiernan Subject: Re: [Histonet] Karnovsky and Roots stain To: Nicole Cosenza Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Karnovsky & Roots is (IMHO) the best histochemical method for choline esterase activity. In muscles, acetylcholinesterase (AChE) is the only such esterase shown by this method, and it is in the subneural apparatus of the motor endplate. Some counterstains (notably silver methods for the innervating axons) can remove the brown copper ferrocyanide product. Another way to show motor endplates is with a method that picks up all esterases. In muscle, the endplate AChE shows up sooner than the enzymes present in all cells. Indigogenic esterase methods can be followed by silver staining of axons. Why do you need or want to use fresh frozen, unfixed tissue sections? This makes no sense in the world of esterase activity histochemistry. There haven't been any developments in this field since the 1960s other than labelled alpha-bungarotoxin and immunohistochemistry. An inexpensive book is Van Noorden CJF & Frederiks WM 1992. Enzyme Histochemistry. Oxford Univ Press and Royal Microscopical Soc. ISBN0198564341. Another one is Lojda, Gossrau & Schiebler 1979. Enzyme histochemistry. Berlin: Springer. ISBN 0387092692. A quick web search indicates that both are available and cost less than $10 second-hand. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Cosenza Date: Thursday, February 3, 2011 18:45 Subject: [Histonet] Karnovsky and Roots stain To: histonet@lists.utsouthwestern.edu > I am looking into a project involving motor end plate staining. > Literature that I've found continually references Karnovsky and > Roots from the 60s. However the papers are not > supplying all the details. > > Does anyone do AchE staining by this method on fresh frozen, > unfixed tissue sections? If so, can I get a more detailed > protocol (fixation steps, washes, etc)? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 4 Feb 2011 15:20:19 -0600 From: "Setlak, Lisa" Subject: RE: [Histonet] Karnovsky and Roots stain To: 'John Kiernan' , Nicole Cosenza Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7683A7@CMHEXCC01MBX.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" We do ACH staining on rectal biopsies to evaluate for ganglion cells for Hirschsprung's disease. Our stain is performed on fresh frozen tissue and I believe it's the Karnovsky method, but I'm not sure. Feel free to email if you are interested in out procedure. Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Friday, February 04, 2011 3:15 PM To: Nicole Cosenza Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Karnovsky and Roots stain Karnovsky & Roots is (IMHO) the best histochemical method for choline esterase activity. In muscles, acetylcholinesterase (AChE) is the only such esterase shown by this method, and it is in the subneural apparatus of the motor endplate. Some counterstains (notably silver methods for the innervating axons) can remove the brown copper ferrocyanide product. Another way to show motor endplates is with a method that picks up all esterases. In muscle, the endplate AChE shows up sooner than the enzymes present in all cells. Indigogenic esterase methods can be followed by silver staining of axons. Why do you need or want to use fresh frozen, unfixed tissue sections? This makes no sense in the world of esterase activity histochemistry. There haven't been any developments in this field since the 1960s other than labelled alpha-bungarotoxin and immunohistochemistry. An inexpensive book is Van Noorden CJF & Frederiks WM 1992. Enzyme Histochemistry. Oxford Univ Press and Royal Microscopical Soc. ISBN0198564341. Another one is Lojda, Gossrau & Schiebler 1979. Enzyme histochemistry. Berlin: Springer. ISBN 0387092692. A quick web search indicates that both are available and cost less than $10 second-hand. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Cosenza Date: Thursday, February 3, 2011 18:45 Subject: [Histonet] Karnovsky and Roots stain To: histonet@lists.utsouthwestern.edu > I am looking into a project involving motor end plate staining. > Literature that I've found continually references Karnovsky and > Roots from the 60s. However the papers are not > supplying all the details. > > Does anyone do AchE staining by this method on fresh frozen, > unfixed tissue sections? If so, can I get a more detailed > protocol (fixation steps, washes, etc)? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 04 Feb 2011 15:53:49 -0600 From: Nicole Cosenza Subject: [Histonet] combined cholinesterase-silver stain To: histonet@lists.utsouthwestern.edu Message-ID: <4D4C756D.4000404@siumed.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I am looking into staining motor end plates. I've come across this combined cholinesterase-silver stain (reference Pestronk and Drachman, 1978). Based on the date of the paper, I'm wondering what the current technique is for this double staining. Anyone currently doing AchE and axon staining on fresh frozen muscle sections? ------------------------------ Message: 10 Date: Fri, 4 Feb 2011 14:04:56 -0800 (PST) From: Akemi Allison Subject: [Histonet] pH Meter To: histonet Message-ID: <506187.89374.qm@web113811.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Happy Friday everyone! I am in the market for a new pH Meter, and was wondering if any of you had any preferences.? I want to keep it simple as possible for?the staff.? Also, I ?don't want to spend a ton of money. One of our fellow histonet subscribers?recommended purchasing a pH Meter that did a? 2-step calibration verses a 3-step.? I've always used a 3-step calibration.? Any thoughts.? ? I realize that it must have a good glass electrode, calibrated daily, prior to use, and all the maintenence must be followed to keep it in good working order. ? If you have any suggestions, please provide the make and model #, and if you have a vendor who provides it such as Fisher or Thermo, that would be an added bonus. Thank you, and have a great weekend! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 11 Date: Fri, 04 Feb 2011 18:03:05 -0600 From: "Ruppert, Amysue" Subject: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" Message-ID: <201102050003.p15030nh007461@spamfilt> Content-Type: text/plain; charset="iso-8859-1" Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ------------------------------ Message: 12 Date: Sat, 05 Feb 2011 00:11:35 -0500 From: John Kiernan Subject: Re: [Histonet] Amylase Digestion for glycogen To: "Ruppert, Amysue" , "histonet@lists.utsouthwestern.edu" Message-ID: <7410bdbf6866.4d4c95b7@uwo.ca> Content-Type: text/plain; charset="iso-8859-1"
Hello,
?We are looking to switch f diastase digestion for glycogen to Amylase digestion. I have new protocol worked up, but one of the Pathologists I work with wo uld like to have an idea of how many labs out there are using Amylase in you use who you are appreciated.

Marshfield Labs
____________ _______________________ 5F _______________________ 5F message may information.? If you destroy the e-mail message are prohibited from retaining, any information contained within. advise of the erroneous delivery by re Thank you for your cooperation.
------------------------------ Message: 13 Date: Fri, 4 Feb 2011 23:22:41 -0800 From: Per Magne Knutsen Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be obtained? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi, I've searched every catalog and website for "Fast Blue" (Diamidino compound 253/50), widely used for neuronal retrograde tracing. One recent paper using this compound is: Porreroa et al "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice", Brain Research, 1345, 59-72 where the authors as many before them cite "Dr. Illing GmbH & Co. KG, Gro??-Umstadt, Germany" as the origin. I have been unable to locate this particular vendor. I've written the authors and am waiting for an answer. In the meanwhile, can anyone on this list help? Kindly, *Per M Knutsen *Department of Physics University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0374 T: +1 858 405 2868 E: pknutsen@ucsd.edu W: http://pmknutsen.blogspot.com * * ------------------------------ Message: 14 Date: Sat, 5 Feb 2011 15:20:01 +0000 (UTC) From: koellingr@comcast.net Subject: Re: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be obtained? To: Per Magne Knutsen Cc: histonet@lists.utsouthwestern.edu Message-ID: <666683375.5867.1296919201956.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Hello Per, Have no particular interest in neuronal retrograde tracing and have certainly never used anything for that. Just general scientific curiosity and a few minutes time awaiting a home project.?? Found Fast Blue (synonym: Diamidino compound 253/50) on Sigma website cat # F5756. Good luck. Ray Ray Koelling PhenoPath Labs Seattle WA ----- Original Message ----- From: "Per Magne Knutsen" To: histonet@lists.utsouthwestern.edu Sent: Friday, February 4, 2011 11:22:41 PM Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be????????????????obtained? Hi, I've searched every catalog and website for "Fast Blue" (Diamidino compound 253/50), widely used for neuronal retrograde tracing. One recent paper using this compound is: Porreroa et al "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice", Brain Research, 1345, 59-72 where the authors as many before them cite "Dr. Illing GmbH & Co. KG, Gro??-Umstadt, Germany" as the origin. I have been unable to locate this particular vendor. I've written the authors and am waiting for an answer. In the meanwhile, can anyone on this list help? Kindly, *Per M Knutsen *Department of Physics University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0374 T: +1 858 405 2868 E: pknutsen@ucsd.edu W: http://pmknutsen.blogspot.com * * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Sat, 5 Feb 2011 08:42:57 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" , AmysueRuppert Message-ID: <228486.12081.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You are dealing with synonyms of the same enzyme but the "amylase" protocol always is slightly more complex. Stick with "diastase" and, please, never use your own saliva for this procedure!!!! Ren? J. --- On Fri, 2/4/11, Ruppert, Amysue wrote: From: Ruppert, Amysue Subject: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 4, 2011, 7:03 PM Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information.? If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.? Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.? Thank you for your cooperation. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Sat, 5 Feb 2011 10:25:21 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] CE To: "'andrea conard'" , Message-ID: <7B33835B42264FAE93FD4F474B9283CE@prueggihctechlt> Content-Type: text/plain; charset="US-ASCII" It was ASCP that had TechSample, they do not have that anymore. The teleconferences from NSH are reasonable and can be done at a distance. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of andrea conard Sent: Friday, February 04, 2011 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] CE I'm looking for some cost effective CE for my staff. Does anyone know if the CAP still has TechSamples? Please email at andrea.conard@atlanticare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 87, Issue 10 **************************************** From emilystebens <@t> gmail.com Mon Feb 7 09:44:48 2011 From: emilystebens <@t> gmail.com (emily stebens) Date: Mon Feb 7 09:44:54 2011 Subject: [Histonet] BCL-1 staining on bone marrow core Message-ID: Does anyone have a protocol or tips for getting bcl-1 to work on commercially decaled bone marrow? From LSebree <@t> uwhealth.org Mon Feb 7 12:13:11 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Feb 7 12:13:36 2011 Subject: [Histonet] Test...please ignore Message-ID: <8C023B4AB999614BA4791BAEB26E273839A1B6@UWHC-MAIL01.uwhis.hosp.wisc.edu> Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From LSebree <@t> uwhealth.org Mon Feb 7 12:16:25 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Feb 7 12:16:35 2011 Subject: [Histonet] CD21 antibody Message-ID: <8C023B4AB999614BA4791BAEB26E273839A1B7@UWHC-MAIL01.uwhis.hosp.wisc.edu> Can anyone out there in Histoland recommend a reliable and robust CD21 for FFPE human tissue? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From settembr <@t> umdnj.edu Mon Feb 7 12:48:06 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Mon Feb 7 12:48:20 2011 Subject: [Histonet] RE: CD21 antibody In-Reply-To: <8C023B4AB999614BA4791BAEB26E273839A1B7@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <8C023B4AB999614BA4791BAEB26E273839A1B7@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: Hi Linda, I found Dako's CD21 mouse antibody reliable. M0784 Dana Settembre University Hospital - UMDNJ 150 Bergen Street Newark, NJ 07103 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, February 07, 2011 1:16 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CD21 antibody Can anyone out there in Histoland recommend a reliable and robust CD21 for FFPE human tissue? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Mon Feb 7 14:18:37 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Mon Feb 7 14:18:52 2011 Subject: [Histonet] CAP ANP.22760 Message-ID: <4D4FF12D020000A800055B98@ns.luhcares.org> hey All, My question is directed to those Hospital labs that have 1(one) DAKO instrument. 1) How are you handling the 'parallel' of this question? 2) What is your validation of the visualization system protocol? 3) How are you running your validation of anti-bodies? We have a few protocols that we have been trying and want to see what others have come up with. Thanks, Matt Lunetta HT(ASCP) Longmont United Hospital From laurie.colbert <@t> huntingtonhospital.com Mon Feb 7 14:49:31 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Feb 7 14:49:35 2011 Subject: [Histonet] CAP ANP.22760 In-Reply-To: <4D4FF12D020000A800055B98@ns.luhcares.org> References: <4D4FF12D020000A800055B98@ns.luhcares.org> Message-ID: <57BE698966D5C54EAE8612E8941D768301268F35@EXCHANGE3.huntingtonhospital.com> Matt, We have Ventana stainers, and I have a comment on your first question and would welcome input from others on this topic. We cannot run "parallel" validations on either the Benchmarks or the Ultras, so I have stated that in my validation procedure. There's nothing I can do about it. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Monday, February 07, 2011 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP ANP.22760 hey All, My question is directed to those Hospital labs that have 1(one) DAKO instrument. 1) How are you handling the 'parallel' of this question? 2) What is your validation of the visualization system protocol? 3) How are you running your validation of anti-bodies? We have a few protocols that we have been trying and want to see what others have come up with. Thanks, Matt Lunetta HT(ASCP) Longmont United Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From theresec <@t> slonepartners.com Mon Feb 7 21:21:19 2011 From: theresec <@t> slonepartners.com (Therese Cook) Date: Mon Feb 7 21:21:25 2011 Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Message-ID: <20110208032121.8D2865331B@qmail2.topechelon.com> Slone Partners client, located This key management position, will to day operations of this busy department. HT and supervisory experience are required. A BS If you are energetic, have great communication positive attitude, this might be the position for you. Qualified candidates should send their resume to Therese [1]theresec@slonepartners.com. If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward your All inquiries are kept confidential. References 1. 3D"mailto:theresec@slonepartners.com?subject=New%20Candidate%20Request%20%28Surgical%20Pathology%20Supervisor%20-%201455%29" 2. 3D"mailto:tara@slonepartners.com?subject=New%20Candidate%20Request%20" From sfonner <@t> labpath.com Tue Feb 8 06:45:29 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Tue Feb 8 06:48:10 2011 Subject: [Histonet] High Complexity Testing Message-ID: <000601cbc78e$117561e0$346025a0$@com> Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! From GDawson <@t> dynacaremilwaukee.com Tue Feb 8 07:12:36 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Feb 8 07:12:46 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: <000601cbc78e$117561e0$346025a0$@com> References: <000601cbc78e$117561e0$346025a0$@com> Message-ID: Sheila, Yes, IHC/ISH is considered high complexity testing. Sounds like you have a real gem for a pathologist...sorry to hear that. Just don't let him/her pay you like a janitor just because that's what he/she thinks that is what you are. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 08, 2011 6:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] High Complexity Testing Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue Feb 8 07:16:50 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Feb 8 07:16:54 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: References: <000601cbc78e$117561e0$346025a0$@com> Message-ID: Sorry for the early morning grammar everyone... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, February 08, 2011 7:13 AM To: 'Sheila Fonner'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High Complexity Testing Sheila, Yes, IHC/ISH is considered high complexity testing. Sounds like you have a real gem for a pathologist...sorry to hear that. Just don't let him/her pay you like a janitor just because that's what he/she thinks that is what you are. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 08, 2011 6:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] High Complexity Testing Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anna.Inman <@t> stmarygj.org Tue Feb 8 08:27:11 2011 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Tue Feb 8 08:27:21 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: References: <000601cbc78e$117561e0$346025a0$@com> Message-ID: <2925AE271EAAD440AF48FCCEB8002D091443E946@smgmail01.smgj.sclhs.net> This is the response I received a year ago from CAP when asked the same question regarding high complexity testing - "Immunohistochemistry staining (manual or automated) is considered specimen processing and is not assigned a test complexity" Has something changed recently? Anna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, February 08, 2011 6:13 AM To: 'Sheila Fonner'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High Complexity Testing Sheila, Yes, IHC/ISH is considered high complexity testing. Sounds like you have a real gem for a pathologist...sorry to hear that. Just don't let him/her pay you like a janitor just because that's what he/she thinks that is what you are. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 08, 2011 6:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] High Complexity Testing Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Robin.Taylor <@t> prexushealth.com Tue Feb 8 09:01:22 2011 From: Robin.Taylor <@t> prexushealth.com (Taylor, Robin) Date: Tue Feb 8 09:01:28 2011 Subject: [Histonet] (no subject) Message-ID: <884A8E132D88314EA5E1FC3BBCA3DA2E9820994C9C@PHPEXMBCLUS.docsgroup.com> Hi guys, For those of you that have been using Thermo nylon biospy bags, what are you doing now that Thermo no longer stocks them? They changed the bags, 2 seams to 3 seams, I tried the new ones, but I don't like them as well as the original ones. I found them hard to get open and several times my biopsies went flying. Can anyone suggest another brand that is as good as the Thermo ones. Thanks so much for your help, Robin Taylor HT Butler County Medical Center Hamilton, OH 513-454-1417 ________________________________ This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From billodonnell <@t> catholichealth.net Tue Feb 8 09:06:33 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Feb 8 09:06:41 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: <000601cbc78e$117561e0$346025a0$@com> References: <000601cbc78e$117561e0$346025a0$@com> Message-ID: I don't actually have an answer, but rather an observation. How many med techs are still doing glusoses in test-tubes, or manual drug screenings or hcg's? It would seem, by deduction that an automated glucose, if only because who it performing it, is a complex test. If measuring a bowel biopsy is now "complex", why should the critical judgements and skills needed to cut a section, place it correctly on a slide and load the machine be considered not complex. If it comes down to "who" is doing it, then all of our efforts to elevate the field, gain higher and more competative wages and education requirements have done little except in isolated regions or laboratories. Morning rant.... Need to start getting more sleep. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 08, 2011 6:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] High Complexity Testing Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From galinadeyneko <@t> yahoo.com Tue Feb 8 09:21:57 2011 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Tue Feb 8 09:22:01 2011 Subject: [Histonet] Re: Histonet Digest, Vol 87, Issue 12 Message-ID: <317141.78782.qm@web33108.mail.mud.yahoo.com> Dear Colleagues, I would like to find chromogen for alkaline phosphatase enzyme labeling. I have used Vulcan fast red from Biocare, but it usually gives me background especially on double stained slides on mouse pancreas, i do KI 67 and BRDU/ Insulin double staining, and ?I use AP enzyme for the pancreatic islets with rabbit S Cruz anti insulin antibody and rabbit--on rodent AP polymer from Biocare Could you please share your experience what? and from what company detection system and chromogen i can use to get rid of background. I appreciate any hints and protocols. Thank you. Galina Deyneko Novartis, Cambridge, MA 617-782-1675 home 617-871-7613 w --- From Beth_Mickley <@t> URMC.Rochester.edu Tue Feb 8 09:30:20 2011 From: Beth_Mickley <@t> URMC.Rochester.edu (Mickley, Beth) Date: Tue Feb 8 09:33:15 2011 Subject: [Histonet] mart-1 staining in Mohs Message-ID: Hello, Does anyone out there stain for mart-1 on their melanoma cases on frozen sections. I work for a Mohs surgeon in NYS and we are interested in doing our own testing in our lab for our melanoma cases. We are currently sending out our melanomas for slow Mohs. Any advice would be greatly appreciated. Thank you! ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Monday, February 07, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Amylase Digestion for glycogen (Tony Henwood) 2. pale stained areas H&E (raghulj@orchidpharma.com) 3. BCL-1 staining on bone marrow core (emily stebens) ---------------------------------------------------------------------- Message: 1 Date: Mon, 7 Feb 2011 06:40:01 +0000 From: Tony Henwood Subject: RE: [Histonet] Amylase Digestion for glycogen To: "'Ruppert, Amysue'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A715706250A@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" Amysue, Our amylase digestion procedure is quite simple (see: Mangan, V-M, Farago, V., Kelly, M., Henwood, A.F., (2002) "An Amylase Reagent with a Long Shelf Life for the removal of Glycogen from Tissue Sections" J. Histotechnol 25:153.) See below for the method: Amylase Reagent Warning: Harmful, contains azide - see MSDS Alpha Amylase from Bacillus Subtilis (Fluka Cat No 10070,) 1g Oxoid PBS Tablets (Cat No BR14a) 1 tablet Distilled water 100ml Sodium Azide 0.1g This solution, once prepared is stored at 4oC when not in use. A recycled antibody dropper bottle (often used in commercial immunoperoxidase kits) is useful for storage and application. Procedure: 1. Dewax and hydrate paraffin sections, hydrate frozen sections. 2. For amylase digestion, place slides on a rack, cover sections with amylase solution and allow to incubate for 10 minutes at room temperature. 3. Wash slides well in water. 4. Place slides in 1% periodic acid 10 minutes. 5. Wash slides well in water. 6. Rinse slides in distilled water. 7. Place in Schiff's reagent 10 minutes. 8. Rinse slides in distilled water and then wash slides in tap water 3 minutes. 9. Counterstain slides with haematoxylin, differentiate and blue. 10. Dehydrate, clear and mount. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ruppert, Amysue Sent: Saturday, 5 February 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amylase Digestion for glycogen Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 2 Date: Mon, 7 Feb 2011 07:30:46 +0000 From: Subject: [Histonet] pale stained areas H&E To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear histonetters, I have pasted two images img001 and img002 dt. 7feb2011 on staining. The images are of mouse kidney 4micron paraffin embedded formalin fixed sections where the hematoxylin stained areas are pale and not uniform. Is it because of over heating or sections getting dried during coverslipping? Kindly help with your suggestions Regards raghul -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, February 05, 2011 11:32 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. veterinary IHC for Ornithobacterium (Jan Shivers) 2. HCN4 staining (Shaw, Sharon) 3. Acetylcholinesterase (mtitford@aol.com) 4. Artifact or dirt on slides using a tape cover slipper? (Nancy) 5. RE: Artifact or dirt on slides using a tape cover slipper? (Setlak, Lisa) 6. RE: Artifact or dirt on slides using a tape cover slipper? (Laurie Colbert) 7. Re: Karnovsky and Roots stain (John Kiernan) 8. RE: Karnovsky and Roots stain (Setlak, Lisa) 9. combined cholinesterase-silver stain (Nicole Cosenza) 10. pH Meter (Akemi Allison) 11. Amylase Digestion for glycogen (Ruppert, Amysue) 12. Re: Amylase Digestion for glycogen (John Kiernan) 13. Where can "Fast Blue" (Diamidino compound 253/50) be obtained? (Per Magne Knutsen) 14. Re: Where can "Fast Blue" (Diamidino compound 253/50) be obtained? (koellingr@comcast.net) 15. Re: Amylase Digestion for glycogen (Rene J Buesa) 16. RE: SPAM-LOW: [Histonet] CE (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Fri, 4 Feb 2011 12:12:01 -0600 From: "Jan Shivers" Subject: [Histonet] veterinary IHC for Ornithobacterium To: "histonet" Message-ID: <40B81232AD4048BA848C9586ABAA9255@auxs.umn.edu> Content-Type: text/plain; charset="iso-8859-1" Does anyone do IHC staining for Ornithobacterium rhinotracheale (ORT), a respiratory pathogen in poultry? I'm trying to find a lab to do it, or a vendor source for antibody. Much thanks in advance, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ------------------------------ Message: 2 Date: Fri, 04 Feb 2011 14:41:38 -0500 From: "Shaw, Sharon" Subject: [Histonet] HCN4 staining To: "histonet@lists.utsouthwestern.edu" Message-ID: <223F1D19A67B3245ADED1F18858077E005077552@S281.admin.wpi.edu> Content-Type: text/plain; charset="us-ascii" Hello, I'm hoping somebody out there can give me some help with HCN4 staining, I was asked to stain HCN4 on mouse heart and it needs to stain the SA node. It is very hard to find that area since the node is very small. I have tried serial sections but not sure I hit the area or if I'm having problems with the stain. The antibody is Rat monoclonal Secondary Alexa Flour 488 goat anti-rat Counter stain is Hoechst I tried frozen and paraffin sections with no luck Any suggestions will help greatly Thanks, Sharon ------------------------------ Message: 3 Date: Fri, 04 Feb 2011 15:06:18 -0500 From: mtitford@aol.com Subject: [Histonet] Acetylcholinesterase To: histonet@lists.utsouthwestern.edu Message-ID: <8CD92BE1E6196A0-1C1C-38BD7@Webmail-d105.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Nicole Cosenza asks about acetylcholinesterase methods. Years and years ago in London we used Koelle's method to demonstrate cholinesterase in muscle and mouse diaphragms. The method we followed was in Pearse E, Histochemistry - Theoretical and Applied. Volume 2. Churchill Livingstone. London 1972. Pages 1312 - 1316 has several different methods. The enzyme was visualized with ammonium sulphide. The tissues were mounted in glycerine jelly. If the method worked too well and we could not see the motor end plates, we adjusted the pH to reduce staining. Dr Filipe went on to publish her own method in Filipe I., Lake B., Histochemistry in Pathology. Churchill Livingstone. London 1983 page 322. In her method, she used osmium to visualise the enzyme, instead of ammonium sulphide. Stain technology used to have articles about the method too. I have not heard of Karnovsky or Roots methods, but lot of different methods were published in the early days of enzyme histochemistry. Hope this helps Michael Titford Pathology - USA Mobile AL ------------------------------ Message: 4 Date: Fri, 4 Feb 2011 12:12:13 -0800 From: "Nancy" Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? To: Message-ID: <003a01cbc4a7$d0054560$700fd020$@com> Content-Type: text/plain; charset="us-ascii" At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com ------------------------------ Message: 5 Date: Fri, 4 Feb 2011 14:22:50 -0600 From: "Setlak, Lisa" Subject: RE: [Histonet] Artifact or dirt on slides using a tape cover slipper? To: 'Nancy' , "histonet@lists.utsouthwestern.edu" Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7683A3@CMHEXCC01MBX.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" We too have the same Coverslipper but we use the Sakura brand tape. We tried the KP tape but did not like it; it seemed to have problems sticking on the slides and we had a few other issues but I don't remember what they were. Could it be that there needs to be more xylene applied to slides- it may be air and dry tissue that you're seeing. Hope this helps. Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Sent: Friday, February 04, 2011 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 4 Feb 2011 12:44:19 -0800 From: "Laurie Colbert" Subject: RE: [Histonet] Artifact or dirt on slides using a tape cover slipper? To: "Nancy" , Message-ID: <57BE698966D5C54EAE8612E8941D76830A699BCD@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Nancy, We get areas that appear brown when the tape doesn't lay flat or adhere to the slide. Most of the time this happens on decal slides or hard tissue where the tissue may be lifted a little. Try recoverslipping. Also, if it's not happening on these types of tissue, you may need more xylene dispensed. Sometimes, we just have to resort to the old-fashioned way of coverslipping with mounting media. I've never used the Klinipath tape. I prefer to stick with Sakura's brand - don't want to take any chances! Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Sent: Friday, February 04, 2011 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell nancy@pathologyarts.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 04 Feb 2011 16:15:03 -0500 From: John Kiernan Subject: Re: [Histonet] Karnovsky and Roots stain To: Nicole Cosenza Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Karnovsky & Roots is (IMHO) the best histochemical method for choline esterase activity. In muscles, acetylcholinesterase (AChE) is the only such esterase shown by this method, and it is in the subneural apparatus of the motor endplate. Some counterstains (notably silver methods for the innervating axons) can remove the brown copper ferrocyanide product. Another way to show motor endplates is with a method that picks up all esterases. In muscle, the endplate AChE shows up sooner than the enzymes present in all cells. Indigogenic esterase methods can be followed by silver staining of axons. Why do you need or want to use fresh frozen, unfixed tissue sections? This makes no sense in the world of esterase activity histochemistry. There haven't been any developments in this field since the 1960s other than labelled alpha-bungarotoxin and immunohistochemistry. An inexpensive book is Van Noorden CJF & Frederiks WM 1992. Enzyme Histochemistry. Oxford Univ Press and Royal Microscopical Soc. ISBN0198564341. Another one is Lojda, Gossrau & Schiebler 1979. Enzyme histochemistry. Berlin: Springer. ISBN 0387092692. A quick web search indicates that both are available and cost less than $10 second-hand. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Cosenza Date: Thursday, February 3, 2011 18:45 Subject: [Histonet] Karnovsky and Roots stain To: histonet@lists.utsouthwestern.edu > I am looking into a project involving motor end plate staining. > Literature that I've found continually references Karnovsky and > Roots from the 60s. However the papers are not > supplying all the details. > > Does anyone do AchE staining by this method on fresh frozen, > unfixed tissue sections? If so, can I get a more detailed > protocol (fixation steps, washes, etc)? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 4 Feb 2011 15:20:19 -0600 From: "Setlak, Lisa" Subject: RE: [Histonet] Karnovsky and Roots stain To: 'John Kiernan' , Nicole Cosenza Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <7111DB39D045004C9CF29E79C71B28BC0CFA7683A7@CMHEXCC01MBX.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" We do ACH staining on rectal biopsies to evaluate for ganglion cells for Hirschsprung's disease. Our stain is performed on fresh frozen tissue and I believe it's the Karnovsky method, but I'm not sure. Feel free to email if you are interested in out procedure. Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Friday, February 04, 2011 3:15 PM To: Nicole Cosenza Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Karnovsky and Roots stain Karnovsky & Roots is (IMHO) the best histochemical method for choline esterase activity. In muscles, acetylcholinesterase (AChE) is the only such esterase shown by this method, and it is in the subneural apparatus of the motor endplate. Some counterstains (notably silver methods for the innervating axons) can remove the brown copper ferrocyanide product. Another way to show motor endplates is with a method that picks up all esterases. In muscle, the endplate AChE shows up sooner than the enzymes present in all cells. Indigogenic esterase methods can be followed by silver staining of axons. Why do you need or want to use fresh frozen, unfixed tissue sections? This makes no sense in the world of esterase activity histochemistry. There haven't been any developments in this field since the 1960s other than labelled alpha-bungarotoxin and immunohistochemistry. An inexpensive book is Van Noorden CJF & Frederiks WM 1992. Enzyme Histochemistry. Oxford Univ Press and Royal Microscopical Soc. ISBN0198564341. Another one is Lojda, Gossrau & Schiebler 1979. Enzyme histochemistry. Berlin: Springer. ISBN 0387092692. A quick web search indicates that both are available and cost less than $10 second-hand. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicole Cosenza Date: Thursday, February 3, 2011 18:45 Subject: [Histonet] Karnovsky and Roots stain To: histonet@lists.utsouthwestern.edu > I am looking into a project involving motor end plate staining. > Literature that I've found continually references Karnovsky and > Roots from the 60s. However the papers are not > supplying all the details. > > Does anyone do AchE staining by this method on fresh frozen, > unfixed tissue sections? If so, can I get a more detailed > protocol (fixation steps, washes, etc)? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 04 Feb 2011 15:53:49 -0600 From: Nicole Cosenza Subject: [Histonet] combined cholinesterase-silver stain To: histonet@lists.utsouthwestern.edu Message-ID: <4D4C756D.4000404@siumed.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I am looking into staining motor end plates. I've come across this combined cholinesterase-silver stain (reference Pestronk and Drachman, 1978). Based on the date of the paper, I'm wondering what the current technique is for this double staining. Anyone currently doing AchE and axon staining on fresh frozen muscle sections? ------------------------------ Message: 10 Date: Fri, 4 Feb 2011 14:04:56 -0800 (PST) From: Akemi Allison Subject: [Histonet] pH Meter To: histonet Message-ID: <506187.89374.qm@web113811.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Happy Friday everyone! I am in the market for a new pH Meter, and was wondering if any of you had any preferences.? I want to keep it simple as possible for?the staff.? Also, I ?don't want to spend a ton of money. One of our fellow histonet subscribers?recommended purchasing a pH Meter that did a? 2-step calibration verses a 3-step.? I've always used a 3-step calibration.? Any thoughts.? ? I realize that it must have a good glass electrode, calibrated daily, prior to use, and all the maintenence must be followed to keep it in good working order. ? If you have any suggestions, please provide the make and model #, and if you have a vendor who provides it such as Fisher or Thermo, that would be an added bonus. Thank you, and have a great weekend! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 11 Date: Fri, 04 Feb 2011 18:03:05 -0600 From: "Ruppert, Amysue" Subject: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" Message-ID: <201102050003.p15030nh007461@spamfilt> Content-Type: text/plain; charset="iso-8859-1" Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ------------------------------ Message: 12 Date: Sat, 05 Feb 2011 00:11:35 -0500 From: John Kiernan Subject: Re: [Histonet] Amylase Digestion for glycogen To: "Ruppert, Amysue" , "histonet@lists.utsouthwestern.edu" Message-ID: <7410bdbf6866.4d4c95b7@uwo.ca> Content-Type: text/plain; charset="iso-8859-1"
Hello,
?We are looking to switch f diastase digestion for glycogen to Amylase digestion. I have new protocol worked up, but one of the Pathologists I work with wo uld like to have an idea of how many labs out there are using Amylase in you use who you are appreciated.

Marshfield Labs
____________ _______________________ 5F _______________________ 5F message may information.? If you destroy the e-mail message are prohibited from retaining, any information contained within. advise of the erroneous delivery by re Thank you for your cooperation.
------------------------------ Message: 13 Date: Fri, 4 Feb 2011 23:22:41 -0800 From: Per Magne Knutsen Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be obtained? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi, I've searched every catalog and website for "Fast Blue" (Diamidino compound 253/50), widely used for neuronal retrograde tracing. One recent paper using this compound is: Porreroa et al "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice", Brain Research, 1345, 59-72 where the authors as many before them cite "Dr. Illing GmbH & Co. KG, Gro??-Umstadt, Germany" as the origin. I have been unable to locate this particular vendor. I've written the authors and am waiting for an answer. In the meanwhile, can anyone on this list help? Kindly, *Per M Knutsen *Department of Physics University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0374 T: +1 858 405 2868 E: pknutsen@ucsd.edu W: http://pmknutsen.blogspot.com * * ------------------------------ Message: 14 Date: Sat, 5 Feb 2011 15:20:01 +0000 (UTC) From: koellingr@comcast.net Subject: Re: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be obtained? To: Per Magne Knutsen Cc: histonet@lists.utsouthwestern.edu Message-ID: <666683375.5867.1296919201956.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Hello Per, Have no particular interest in neuronal retrograde tracing and have certainly never used anything for that. Just general scientific curiosity and a few minutes time awaiting a home project.?? Found Fast Blue (synonym: Diamidino compound 253/50) on Sigma website cat # F5756. Good luck. Ray Ray Koelling PhenoPath Labs Seattle WA ----- Original Message ----- From: "Per Magne Knutsen" To: histonet@lists.utsouthwestern.edu Sent: Friday, February 4, 2011 11:22:41 PM Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be????????????????obtained? Hi, I've searched every catalog and website for "Fast Blue" (Diamidino compound 253/50), widely used for neuronal retrograde tracing. One recent paper using this compound is: Porreroa et al "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice", Brain Research, 1345, 59-72 where the authors as many before them cite "Dr. Illing GmbH & Co. KG, Gro??-Umstadt, Germany" as the origin. I have been unable to locate this particular vendor. I've written the authors and am waiting for an answer. In the meanwhile, can anyone on this list help? Kindly, *Per M Knutsen *Department of Physics University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093-0374 T: +1 858 405 2868 E: pknutsen@ucsd.edu W: http://pmknutsen.blogspot.com * * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Sat, 5 Feb 2011 08:42:57 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" , AmysueRuppert Message-ID: <228486.12081.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You are dealing with synonyms of the same enzyme but the "amylase" protocol always is slightly more complex. Stick with "diastase" and, please, never use your own saliva for this procedure!!!! Ren? J. --- On Fri, 2/4/11, Ruppert, Amysue wrote: From: Ruppert, Amysue Subject: [Histonet] Amylase Digestion for glycogen To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 4, 2011, 7:03 PM Hello, We are looking to switch from malt diastase digestion for glycogen to Amylase digestion. I have the new protocol worked up, but one of the Pathologists I work with would like to have an idea of how many labs out there are using Amylase instead of malt diastase for their PAS/D method. If you use amylase for the PAS/D method, could you please let me know who you are and the institiution? Much appreciated. amysue ruppert Histology lab Marshfield Labs Marshfield WI ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information.? If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.? Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.? Thank you for your cooperation. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Sat, 5 Feb 2011 10:25:21 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] CE To: "'andrea conard'" , Message-ID: <7B33835B42264FAE93FD4F474B9283CE@prueggihctechlt> Content-Type: text/plain; charset="US-ASCII" It was ASCP that had TechSample, they do not have that anymore. The teleconferences from NSH are reasonable and can be done at a distance. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of andrea conard Sent: Friday, February 04, 2011 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] CE I'm looking for some cost effective CE for my staff. Does anyone know if the CAP still has TechSamples? Please email at andrea.conard@atlanticare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 87, Issue 10 **************************************** ------------------------------ Message: 3 Date: Mon, 7 Feb 2011 10:44:48 -0500 From: emily stebens Subject: [Histonet] BCL-1 staining on bone marrow core To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Does anyone have a protocol or tips for getting bcl-1 to work on commercially decaled bone marrow? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 87, Issue 12 **************************************** From sspinette <@t> ric.edu Tue Feb 8 09:51:43 2011 From: sspinette <@t> ric.edu (Spinette, Sarah) Date: Tue Feb 8 09:51:46 2011 Subject: [Histonet] mouse skeletal muscle frozen section prep Message-ID: <06B73B03-3C30-4027-8825-0924F57A0BBE@mimectl> I am new to working with mouse skeletal muscle and have been reading about various methods for preparing and freezing tissue for sectioning. I have heard of the benefits of coating the tissue in talc prior to freezing but have not yet seen a single protocol that describes the use of talc and OCT or other medium IN plastic molds, can you still use talc and then plunge the tissue into cold OCT in the mold end then plunge the entire block into LiN2 (we do not have isopentane)? Also, can anyone comment about when and how they choose to fix the tissue first and if they use paraformaldehyde before freezing? Thank you! From rjbuesa <@t> yahoo.com Tue Feb 8 10:31:58 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 8 10:32:03 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: <000601cbc78e$117561e0$346025a0$@com> Message-ID: <329726.98088.qm@web65712.mail.ac4.yahoo.com> When a "machine" is doing the test, there are stringent provisions as to the preparation and validations of the test. Done manually, it requires a trained technologists and, yes, they are high complexity tests (both IHC and FISH, and their variations). Ren? J. --- On Tue, 2/8/11, Sheila Fonner wrote: From: Sheila Fonner Subject: [Histonet] High Complexity Testing To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 7:45 AM Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs.? Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work.? Can anyone help me out with some guidelines, literature, etc. that says otherwise?? I would really appreciate it.? We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Feb 8 10:57:56 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Feb 8 10:58:02 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: <329726.98088.qm@web65712.mail.ac4.yahoo.com> References: <000601cbc78e$117561e0$346025a0$@com> <329726.98088.qm@web65712.mail.ac4.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB7194516A69E@EVS1.archildrens.org> While the test is high complexity it is the READING of the test by the pathologist that determines its complexity. Because histotechs do not report the results our part of this test is not high complexity. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 08, 2011 10:32 AM To: histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: Re: [Histonet] High Complexity Testing When a "machine" is doing the test, there are stringent provisions as to the preparation and validations of the test. Done manually, it requires a trained technologists and, yes, they are high complexity tests (both IHC and FISH, and their variations). Ren? J. --- On Tue, 2/8/11, Sheila Fonner wrote: From: Sheila Fonner Subject: [Histonet] High Complexity Testing To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 7:45 AM Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs.? Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work.? Can anyone help me out with some guidelines, literature, etc. that says otherwise?? I would really appreciate it.? We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From bakevictoria <@t> gmail.com Tue Feb 8 10:58:55 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Feb 8 10:59:00 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: References: <000601cbc78e$117561e0$346025a0$@com> Message-ID: The performance of ISH/IHC is in many laboratories considered high complexity testing - however - as the technologist doing the work does not make the diagnosis it is considered to be a part of specimen processing. (How's that for political double talking!) Use of automation in these procedures provides standardization of the protocols, but it is still a 'machine' it would not know how to recognize a staining or processing issue along with other possible faults. The purpose of automation was to provide standardization and consistent reproducibility of protocols, reduce antibody/reagent amounts, free the technologist for other needs in the lab and reduce turn around time required for this procedure. In essence it is hands off BUT not brains off. Short history is that when CLIA and CAP first started doing these ratings of low - high complexity testing Histology was a grey/special area in that the staff performed the technical aspect of the work, but did not sign off or make the diagnosis. Since then we have had revisions in CLIA and even with CAP guidelines there is still an aspect of subjectivity based upon the inspector's interpretation of the guidelines. It's frustrating, infuriating at and at times totally exasperating as you are trying to comply with all the requirements and regulations that are supposed to be clear. If your pathologist is considering all Histology work as low complexity I would wonder why they thought that? Is the lab participating in any of the CAP QA/QI programs? Does he/she participate in doing any In services with the staff? Do they interact with the staff and share anything with them in regards to the work or any specific concerns they may have? Does the department have reference material available to the staff for further study or understanding of the work they do and how important it is to patient care? Is the department willing to assist with $$'s for in-house CE courses or local Histology societies to further the laboratory staff development? One thing that I would wonder most is if they think that what you do is 'low complexity' could they go into the lab and perform the work themselves? Okay my morning rant is complete --- now I guess I should take my Estrogen ;-) If I have offended anyone, I apologize in advance. Hope everyone has a nice day. Vikki On Tue, Feb 8, 2011 at 10:06 AM, O'Donnell, Bill < billodonnell@catholichealth.net> wrote: > I don't actually have an answer, but rather an observation. > > How many med techs are still doing glusoses in test-tubes, or manual > drug screenings or hcg's? It would seem, by deduction that an automated > glucose, if only because who it performing it, is a complex test. If > measuring a bowel biopsy is now "complex", why should the critical > judgements and skills needed to cut a section, place it correctly on a > slide and load the machine be considered not complex. > > If it comes down to "who" is doing it, then all of our efforts to > elevate the field, gain higher and more competative wages and education > requirements have done little except in isolated regions or > laboratories. > > Morning rant.... Need to start getting more sleep. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila > Fonner > Sent: Tuesday, February 08, 2011 6:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] High Complexity Testing > > Hello All, > > > > I would really appreciate it if anyone has information on whether > IHC/ISH are considered high complexity testing for histotechs. Our > pathologist believes that ALL histology low complexity testing since a > "machine" is doing the work. Can anyone help me out with some > guidelines, literature, etc. that says otherwise? I would really > appreciate it. We just want to know which one it is. > > > > Thanks so much Histoland! > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Tue Feb 8 11:09:20 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Feb 8 11:09:25 2011 Subject: [Histonet] RELIA Special Histology Job Alert - Histology Trainer needed in Chicago. Can you help? Message-ID: Hi Histonetters! I hope everyone is having a great day and staying warm! I have a new position that I am pretty excited about and thought I would run it by you guys and see if anyone was interested or knew of anyone who might be interested. Here are the details: Histology Trainer Full time permanent M-F in house training specialist for national company. excellent pay, benefits and relocation assistance. My client is looking for someone with a strong background in histology and great training skills with the outgoing personality to match! IHC knowledge is desired as well. Do you want to get out of the lab? Do you know anyone who wants to get out of the lab? For more details please contact me. You can reach me toll free at 866-607-3542 or by e-mail at relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From akbitting <@t> geisinger.edu Tue Feb 8 11:13:05 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Feb 8 11:18:43 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: References: <000601cbc78e$117561e0$346025a0$@com> Message-ID: <4D513352.2B7F.00C9.1@geisinger.edu> That was a hole in 1, Vikki. Viva la rant!! >>> Victoria Baker 2/8/2011 11:58 AM >>> The performance of ISH/IHC is in many laboratories considered high complexity testing - however - as the technologist doing the work does not make the diagnosis it is considered to be a part of specimen processing. (How's that for political double talking!) Use of automation in these procedures provides standardization of the protocols, but it is still a 'machine' it would not know how to recognize a staining or processing issue along with other possible faults. The purpose of automation was to provide standardization and consistent reproducibility of protocols, reduce antibody/reagent amounts, free the technologist for other needs in the lab and reduce turn around time required for this procedure. In essence it is hands off BUT not brains off. Short history is that when CLIA and CAP first started doing these ratings of low - high complexity testing Histology was a grey/special area in that the staff performed the technical aspect of the work, but did not sign off or make the diagnosis. Since then we have had revisions in CLIA and even with CAP guidelines there is still an aspect of subjectivity based upon the inspector's interpretation of the guidelines. It's frustrating, infuriating at and at times totally exasperating as you are trying to comply with all the requirements and regulations that are supposed to be clear. If your pathologist is considering all Histology work as low complexity I would wonder why they thought that? Is the lab participating in any of the CAP QA/QI programs? Does he/she participate in doing any In services with the staff? Do they interact with the staff and share anything with them in regards to the work or any specific concerns they may have? Does the department have reference material available to the staff for further study or understanding of the work they do and how important it is to patient care? Is the department willing to assist with $$'s for in-house CE courses or local Histology societies to further the laboratory staff development? One thing that I would wonder most is if they think that what you do is 'low complexity' could they go into the lab and perform the work themselves? Okay my morning rant is complete --- now I guess I should take my Estrogen ;-) If I have offended anyone, I apologize in advance. Hope everyone has a nice day. Vikki On Tue, Feb 8, 2011 at 10:06 AM, O'Donnell, Bill < billodonnell@catholichealth.net> wrote: > I don't actually have an answer, but rather an observation. > > How many med techs are still doing glusoses in test-tubes, or manual > drug screenings or hcg's? It would seem, by deduction that an automated > glucose, if only because who it performing it, is a complex test. If > measuring a bowel biopsy is now "complex", why should the critical > judgements and skills needed to cut a section, place it correctly on a > slide and load the machine be considered not complex. > > If it comes down to "who" is doing it, then all of our efforts to > elevate the field, gain higher and more competative wages and education > requirements have done little except in isolated regions or > laboratories. > > Morning rant.... Need to start getting more sleep. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila > Fonner > Sent: Tuesday, February 08, 2011 6:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] High Complexity Testing > > Hello All, > > > > I would really appreciate it if anyone has information on whether > IHC/ISH are considered high complexity testing for histotechs. Our > pathologist believes that ALL histology low complexity testing since a > "machine" is doing the work. Can anyone help me out with some > guidelines, literature, etc. that says otherwise? I would really > appreciate it. We just want to know which one it is. > > > > Thanks so much Histoland! > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From TGoins <@t> mt.gov Tue Feb 8 11:22:04 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Feb 8 11:22:16 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: <25A4DE08332B19499904459F00AAACB7194516A69E@EVS1.archildrens.org> References: <000601cbc78e$117561e0$346025a0$@com> <329726.98088.qm@web65712.mail.ac4.yahoo.com> <25A4DE08332B19499904459F00AAACB7194516A69E@EVS1.archildrens.org> Message-ID: I must disagree with this assessment of what makes a test complex. If the test is done properly [the responsibility of the technologist] then the reading to the test is a visual determination that requires experience on the part of the pathologist, but if the test is not done properly, will the pathologist be able to tell the technologist what to do to fix the problem? Where's the Tylenol? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, February 08, 2011 9:58 AM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: RE: [Histonet] High Complexity Testing While the test is high complexity it is the READING of the test by the pathologist that determines its complexity. Because histotechs do not report the results our part of this test is not high complexity. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 08, 2011 10:32 AM To: histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: Re: [Histonet] High Complexity Testing When a "machine" is doing the test, there are stringent provisions as to the preparation and validations of the test. Done manually, it requires a trained technologists and, yes, they are high complexity tests (both IHC and FISH, and their variations). Ren? J. --- On Tue, 2/8/11, Sheila Fonner wrote: From: Sheila Fonner Subject: [Histonet] High Complexity Testing To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 7:45 AM Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs.? Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work.? Can anyone help me out with some guidelines, literature, etc. that says otherwise?? I would really appreciate it.? We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Feb 8 11:24:32 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 8 11:24:37 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: References: <000601cbc78e$117561e0$346025a0$@com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A439@CHEXCMS10.one.ads.che.org> Honey... Estrogen won't do a thing!!! j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, February 08, 2011 11:59 To: O'Donnell, Bill Cc: Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High Complexity Testing The performance of ISH/IHC is in many laboratories considered high complexity testing - however - as the technologist doing the work does not make the diagnosis it is considered to be a part of specimen processing. (How's that for political double talking!) Use of automation in these procedures provides standardization of the protocols, but it is still a 'machine' it would not know how to recognize a staining or processing issue along with other possible faults. The purpose of automation was to provide standardization and consistent reproducibility of protocols, reduce antibody/reagent amounts, free the technologist for other needs in the lab and reduce turn around time required for this procedure. In essence it is hands off BUT not brains off. Short history is that when CLIA and CAP first started doing these ratings of low - high complexity testing Histology was a grey/special area in that the staff performed the technical aspect of the work, but did not sign off or make the diagnosis. Since then we have had revisions in CLIA and even with CAP guidelines there is still an aspect of subjectivity based upon the inspector's interpretation of the guidelines. It's frustrating, infuriating at and at times totally exasperating as you are trying to comply with all the requirements and regulations that are supposed to be clear. If your pathologist is considering all Histology work as low complexity I would wonder why they thought that? Is the lab participating in any of the CAP QA/QI programs? Does he/she participate in doing any In services with the staff? Do they interact with the staff and share anything with them in regards to the work or any specific concerns they may have? Does the department have reference material available to the staff for further study or understanding of the work they do and how important it is to patient care? Is the department willing to assist with $$'s for in-house CE courses or local Histology societies to further the laboratory staff development? One thing that I would wonder most is if they think that what you do is 'low complexity' could they go into the lab and perform the work themselves? Okay my morning rant is complete --- now I guess I should take my Estrogen ;-) If I have offended anyone, I apologize in advance. Hope everyone has a nice day. Vikki On Tue, Feb 8, 2011 at 10:06 AM, O'Donnell, Bill < billodonnell@catholichealth.net> wrote: > I don't actually have an answer, but rather an observation. > > How many med techs are still doing glusoses in test-tubes, or manual > drug screenings or hcg's? It would seem, by deduction that an > automated glucose, if only because who it performing it, is a complex > test. If measuring a bowel biopsy is now "complex", why should the > critical judgements and skills needed to cut a section, place it > correctly on a slide and load the machine be considered not complex. > > If it comes down to "who" is doing it, then all of our efforts to > elevate the field, gain higher and more competative wages and > education requirements have done little except in isolated regions or > laboratories. > > Morning rant.... Need to start getting more sleep. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila > Fonner > Sent: Tuesday, February 08, 2011 6:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] High Complexity Testing > > Hello All, > > > > I would really appreciate it if anyone has information on whether > IHC/ISH are considered high complexity testing for histotechs. Our > pathologist believes that ALL histology low complexity testing since a > "machine" is doing the work. Can anyone help me out with some > guidelines, literature, etc. that says otherwise? I would really > appreciate it. We just want to know which one it is. > > > > Thanks so much Histoland! > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Joe.Milano <@t> uphs.upenn.edu Tue Feb 8 11:24:57 2011 From: Joe.Milano <@t> uphs.upenn.edu (Milano, Joe) Date: Tue Feb 8 11:25:01 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D091443E946@smgmail01.smgj.sclhs.net> References: <000601cbc78e$117561e0$346025a0$@com> <2925AE271EAAD440AF48FCCEB8002D091443E946@smgmail01.smgj.sclhs.net> Message-ID: <780ACC3A56F3074B9F158813AF085A4E15F4E3@uphmasphi007.UPHS.PENNHEALTH.PRV> We were recently inspected and were told the same thing by CAP about 4 months ago. Joe Milano -----Original Message----- From: Inman, Anna [mailto:Anna.Inman@stmarygj.org] Sent: Tuesday, February 08, 2011 9:27 AM To: Dawson, Glen; Sheila Fonner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High Complexity Testing This is the response I received a year ago from CAP when asked the same question regarding high complexity testing - "Immunohistochemistry staining (manual or automated) is considered specimen processing and is not assigned a test complexity" Has something changed recently? Anna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, February 08, 2011 6:13 AM To: 'Sheila Fonner'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High Complexity Testing Sheila, Yes, IHC/ISH is considered high complexity testing. Sounds like you have a real gem for a pathologist...sorry to hear that. Just don't let him/her pay you like a janitor just because that's what he/she thinks that is what you are. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 08, 2011 6:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] High Complexity Testing Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Bonnie.Whitaker <@t> osumc.edu Tue Feb 8 11:43:55 2011 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Tue Feb 8 11:44:00 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: References: <000601cbc78e$117561e0$346025a0$@com> <329726.98088.qm@web65712.mail.ac4.yahoo.com> <25A4DE08332B19499904459F00AAACB7194516A69E@EVS1.archildrens.org> Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670617083E86@EXMBOX-VP05.OSUMC.EDU> Hi All, There is a difference in performing a task (immunostaining) that is complex, and performing "high complexity testing" as the CLIA regulations govern. Yes, staining is a complex task, and it requires knowlegable techs to ensure that it is properly done, and to troubleshoot difficulties when necessary. It is "high complexity testing" because "testing personnel" in anatomic pathology are pathologists (and the non-physician people performing gross examinations, who must meet "high complexity testing personnel" requirements. "Testing personnel" as defined by CLIA, are the people that report results of that test, not people who perform other related duties. That's my explanation of the whole mess. Bonnie Whitaker AP Operations Director Ohio State University Medical Center Department of Pathology 614.293.5048 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Tuesday, February 08, 2011 12:22 PM To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: RE: [Histonet] High Complexity Testing I must disagree with this assessment of what makes a test complex. If the test is done properly [the responsibility of the technologist] then the reading to the test is a visual determination that requires experience on the part of the pathologist, but if the test is not done properly, will the pathologist be able to tell the technologist what to do to fix the problem? Where's the Tylenol? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, February 08, 2011 9:58 AM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: RE: [Histonet] High Complexity Testing While the test is high complexity it is the READING of the test by the pathologist that determines its complexity. Because histotechs do not report the results our part of this test is not high complexity. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 08, 2011 10:32 AM To: histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: Re: [Histonet] High Complexity Testing When a "machine" is doing the test, there are stringent provisions as to the preparation and validations of the test. Done manually, it requires a trained technologists and, yes, they are high complexity tests (both IHC and FISH, and their variations). Ren? J. --- On Tue, 2/8/11, Sheila Fonner wrote: From: Sheila Fonner Subject: [Histonet] High Complexity Testing To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 7:45 AM Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs.? Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work.? Can anyone help me out with some guidelines, literature, etc. that says otherwise?? I would really appreciate it.? We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Tue Feb 8 12:04:36 2011 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Feb 8 12:04:41 2011 Subject: [Histonet] mouse skeletal muscle frozen section prep In-Reply-To: <06B73B03-3C30-4027-8825-0924F57A0BBE@mimectl> References: <06B73B03-3C30-4027-8825-0924F57A0BBE@mimectl> Message-ID: I've frozen numerous rodent (including mouse) skeletal muscle tissue for sectioning. I've never heard of using talc before. I just blot any moisture off the tissue with a paper towel or Kimwipe. I usually do not fix the tissue beforehand but after sectioning, on the slide. To freeze, I just immerse the tissue into a cryomold filled with OCT and freeze over liquid nitrogen vapors or, since you don't have isopentane, in a slurry of dry ice/isopropanol (don't let the alcohol spill over into the freezing OCT, however, or you'll have to scrape it off as alcohol won't freeze). Or you can slowly dip the mold into some liquid nitrogen. I wouldn't just throw it in, however, as I used to do that and ended up with half my OCT blocks with cracks in them, right through the tissue. Hope this helps! I'm sure others on Histonet have frozen mouse skeletal muscle as well. Regards, Merced --On Tuesday, February 08, 2011 10:51 AM -0500 "Spinette, Sarah" wrote: > I am new to working with mouse skeletal muscle and have been reading > about various methods for preparing and freezing tissue for sectioning. > > I have heard of the benefits of coating the tissue in talc prior to > freezing but have not yet seen a single protocol that describes the use > of talc and OCT or other medium IN plastic molds, can you still use talc > and then plunge the tissue into cold OCT in the mold end then plunge the > entire block into LiN2 (we do not have isopentane)? > > Also, can anyone comment about when and how they choose to fix the tissue > first and if they use paraformaldehyde before freezing? > > Thank you! > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Timothy.Morken <@t> ucsfmedctr.org Tue Feb 8 11:53:02 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Tue Feb 8 12:14:13 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: <000601cbc78e$117561e0$346025a0$@com> References: <000601cbc78e$117561e0$346025a0$@com> Message-ID: The CLIA criteria are below. Note that the entire test is considered to be the combination of pre-analytic, analytic and post-analytic. It does not matter how the test is performed (manual or automated) because the FDA determines complexity of a "test" as sold by a vendor. It goes with the test, not the particular lab that performs the test. So the lab is said to perform "high complexity testing." If it does so then the personnel in the lab have to meet certain standards. Below is the link to the FDA database of tests. Enter Dako and you will see many of their antibodies, secondary kits, etc, all listed as high complexity tests. Not all tests (antibodies) are listed because most used in the IHC lab are class I exempt (don't need FDA approval for marketing). They are ancillary tests. However, antibodies like estrogen receptor are listed since they require FDA approval for marketing as a test. http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCLIA/search.cfm The question is, who is "performing" the test? Is it the lab tech who does the lab work? Or the pathologist who interprets the results? Or a combination? Who is it that requires specialized knowledge and/or training? ++++++++++++++++++++++++++++ CLIA Categorization Criteria (http://www.fda.gov/MedicalDevices/DeviceRegulationandGuidance/IVDRegulatoryAssistance/ucm124208.htm) Each specific laboratory test system, assay, and examination is graded for level of complexity by assigning scores of 1, 2, or 3 for each of the seven criteria listed below. A score of 1 indicates the lowest level of complexity, and the score of 3 indicates the highest level. These scores are totaled. Test systems, assays or examinations receiving scores of 12 or less are categorized as moderate complexity, while those receiving scores above 12 are categorized as high complexity. Note: A score of 2 will be assigned to a criteria heading when the characteristics for a particular test are intermediate between the descriptions listed for scores of 1 and 3 Tests may also be categorized as waived.1 Criteria for Categorization (1) Knowledge. Score 1. (A) Minimal scientific and technical knowledge is required to perform the test; and (B) Knowledge required to perform the test may be obtained through on-the-job instruction. Score 3. Specialized scientific and technical knowledge is essential to perform preanalytic, analytic or postanalytic phases of the testing. (2) Training and experience. Score 1. (A) Minimal training is required for preanalytic, analytic and postanalytic phases of the testing process; and (B) Limited experience is required to perform the test. Score 3. (A) Specialized training is essential to perform the preanalytic, analytic or postanalytic testing process; or Substantial experience may be necessary for analytic test performance. (3) Reagents and materials preparation. Score 1. (A) Reagents and materials are generally stable and reliable; and (B) Reagents and materials are prepackaged, or premeasured, or require no special handling, precautions or storage conditions. Score 3. (A) Reagents and materials may be labile and may require special handling to assure reliability; or (B) Reagents and materials preparation may include manual steps such as gravimetric or volumetric measurements. (4) Characteristics of operational steps. Score 1. Operational steps are either automatically executed (such as pipetting, temperature monitoring, or timing of steps), or are easily controlled. Score 3. Operational steps in the testing process require close monitoring or control, and may require special specimen preparation,precise temperature control or timing of procedural steps, accuratepipetting, or extensive calculations. (5) Calibration, quality control, and proficiency testing materials. Score 1. (A) Calibration materials are stable and readily available; (B) Quality control materials are stable and readily available; and (C) External proficiency testing materials, when available, are stable. Score 3. (A) Calibration materials, if available, may be labile; (B) Quality control materials may be labile, or not available; or (C) External proficiency testing materials, if available, may be labile. (6) Test system troubleshooting and equipment maintenance. Score 1. (A) Test system troubleshooting is automatic or self-correcting, or clearly described or requires minimal judgment; and (B) Equipment maintenance is provided by the manufacturer, is seldom needed, or can easily be performed. Score 3. (A) Troubleshooting is not automatic and requires decision-making and direct intervention to resolve most problems; or (B) Maintenance requires special knowledge, skills, and abilities. (7) Interpretation and judgment. Score 1. (A) Minimal interpretation and judgment are required to perform preanalytic, analytic and postanalytic processes; and (B) Resolution of problems requires limited independent interpretation and judgment; and Score 3. (A) Extensive independent interpretation and judgment are required to perform the preanalytic, analytic or postanalytic processes; and (B) Resolution of problems requires extensive interpretation and judgment. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 08, 2011 4:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] High Complexity Testing Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Tue Feb 8 12:17:15 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Feb 8 12:22:07 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: <6B106EE8C8AAEF449DEA97921DEC11670617083E86@EXMBOX-VP05.OSUMC.EDU> References: <000601cbc78e$117561e0$346025a0$@com> <329726.98088.qm@web65712.mail.ac4.yahoo.com> <25A4DE08332B19499904459F00AAACB7194516A69E@EVS1.archildrens.org> <6B106EE8C8AAEF449DEA97921DEC11670617083E86@EXMBOX-VP05.OSUMC.EDU> Message-ID: I should not have included CLIA in my e-mail as it would seem it has clouded things a little. I do apologize. Initially when these issues and guidelines came about CLIA and CAP dovetailed as far as Histology was concerned. Shelia you were looking for contacts that would help you with getting a more solid base to meet these regulations. If you go to the CAP website and click on the IHC link you will find links and publications to assist you. I would recommend that you contact the Applied Immunohistochemistry society as well. NSH or your state/regional society may also have additional information. Should I see something else in my searches I will most willingly forwarded them to you. Vikki On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie wrote: > Hi All, > > There is a difference in performing a task (immunostaining) that is > complex, and performing "high complexity testing" as the CLIA regulations > govern. > > Yes, staining is a complex task, and it requires knowlegable techs to > ensure that it is properly done, and to troubleshoot difficulties when > necessary. > > It is "high complexity testing" because "testing personnel" in anatomic > pathology are pathologists (and the non-physician people performing gross > examinations, who must meet "high complexity testing personnel" > requirements. > > "Testing personnel" as defined by CLIA, are the people that report results > of that test, not people who perform other related duties. > > That's my explanation of the whole mess. > > Bonnie Whitaker > AP Operations Director > Ohio State University Medical Center > Department of Pathology > 614.293.5048 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: Tuesday, February 08, 2011 12:22 PM > To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; > Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > I must disagree with this assessment of what makes a test complex. If the > test is done properly [the responsibility of the technologist] then the > reading to the test is a visual determination that requires experience on > the part of the pathologist, but if the test is not done properly, will the > pathologist be able to tell the technologist what to do to fix the problem? > > Where's the Tylenol? > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Tuesday, February 08, 2011 9:58 AM > To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > While the test is high complexity it is the READING of the test by the > pathologist that determines its complexity. Because histotechs do not > report the results our part of this test is not high complexity. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, February 08, 2011 10:32 AM > To: histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: Re: [Histonet] High Complexity Testing > > When a "machine" is doing the test, there are stringent provisions as to > the preparation and validations of the test. > Done manually, it requires a trained technologists and, yes, they are high > complexity tests (both IHC and FISH, and their variations). > Ren? J. > > --- On Tue, 2/8/11, Sheila Fonner wrote: > > > From: Sheila Fonner > Subject: [Histonet] High Complexity Testing > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 8, 2011, 7:45 AM > > > Hello All, > > > > I would really appreciate it if anyone has information on whether IHC/ISH > are considered high complexity testing for histotechs. Our pathologist > believes that ALL histology low complexity testing since a "machine" is > doing the work. Can anyone help me out with some guidelines, literature, > etc. that says otherwise? I would really appreciate it. We just want to > know which one it is. > > > > Thanks so much Histoland! > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message is > not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it from > your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From GDawson <@t> dynacaremilwaukee.com Tue Feb 8 12:40:07 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Feb 8 12:40:13 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: References: <000601cbc78e$117561e0$346025a0$@com> <329726.98088.qm@web65712.mail.ac4.yahoo.com> <25A4DE08332B19499904459F00AAACB7194516A69E@EVS1.archildrens.org> <6B106EE8C8AAEF449DEA97921DEC11670617083E86@EXMBOX-VP05.OSUMC.EDU> Message-ID: Alright, if IHC is not high complexity testing, CAP should cut that massive part of their inspection in half and concentrate more on the pathologists' ability to accurately interpret the staining. Too much CAP regulation, Proficiency Testing & validation requirements involved if all IHC is is part of "Processing". My Opinion, Glen A. Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, February 08, 2011 12:17 PM To: Whitaker, Bonnie Cc: Horn, Hazel V; Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High Complexity Testing I should not have included CLIA in my e-mail as it would seem it has clouded things a little. I do apologize. Initially when these issues and guidelines came about CLIA and CAP dovetailed as far as Histology was concerned. Shelia you were looking for contacts that would help you with getting a more solid base to meet these regulations. If you go to the CAP website and click on the IHC link you will find links and publications to assist you. I would recommend that you contact the Applied Immunohistochemistry society as well. NSH or your state/regional society may also have additional information. Should I see something else in my searches I will most willingly forwarded them to you. Vikki On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie wrote: > Hi All, > > There is a difference in performing a task (immunostaining) that is > complex, and performing "high complexity testing" as the CLIA regulations > govern. > > Yes, staining is a complex task, and it requires knowlegable techs to > ensure that it is properly done, and to troubleshoot difficulties when > necessary. > > It is "high complexity testing" because "testing personnel" in anatomic > pathology are pathologists (and the non-physician people performing gross > examinations, who must meet "high complexity testing personnel" > requirements. > > "Testing personnel" as defined by CLIA, are the people that report results > of that test, not people who perform other related duties. > > That's my explanation of the whole mess. > > Bonnie Whitaker > AP Operations Director > Ohio State University Medical Center > Department of Pathology > 614.293.5048 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: Tuesday, February 08, 2011 12:22 PM > To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; > Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > I must disagree with this assessment of what makes a test complex. If the > test is done properly [the responsibility of the technologist] then the > reading to the test is a visual determination that requires experience on > the part of the pathologist, but if the test is not done properly, will the > pathologist be able to tell the technologist what to do to fix the problem? > > Where's the Tylenol? > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Tuesday, February 08, 2011 9:58 AM > To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > While the test is high complexity it is the READING of the test by the > pathologist that determines its complexity. Because histotechs do not > report the results our part of this test is not high complexity. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, February 08, 2011 10:32 AM > To: histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: Re: [Histonet] High Complexity Testing > > When a "machine" is doing the test, there are stringent provisions as to > the preparation and validations of the test. > Done manually, it requires a trained technologists and, yes, they are high > complexity tests (both IHC and FISH, and their variations). > Ren? J. > > --- On Tue, 2/8/11, Sheila Fonner wrote: > > > From: Sheila Fonner > Subject: [Histonet] High Complexity Testing > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 8, 2011, 7:45 AM > > > Hello All, > > > > I would really appreciate it if anyone has information on whether IHC/ISH > are considered high complexity testing for histotechs. Our pathologist > believes that ALL histology low complexity testing since a "machine" is > doing the work. Can anyone help me out with some guidelines, literature, > etc. that says otherwise? I would really appreciate it. We just want to > know which one it is. > > > > Thanks so much Histoland! > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message is > not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it from > your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Tue Feb 8 13:16:52 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Feb 8 13:17:20 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: References: <000601cbc78e$117561e0$346025a0$@com> <329726.98088.qm@web65712.mail.ac4.yahoo.com> <25A4DE08332B19499904459F00AAACB7194516A69E@EVS1.archildrens.org> <6B106EE8C8AAEF449DEA97921DEC11670617083E86@EXMBOX-VP05.OSUMC.EDU> Message-ID: I completely agree!!! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, February 08, 2011 1:40 PM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High Complexity Testing Alright, if IHC is not high complexity testing, CAP should cut that massive part of their inspection in half and concentrate more on the pathologists' ability to accurately interpret the staining. Too much CAP regulation, Proficiency Testing & validation requirements involved if all IHC is is part of "Processing". My Opinion, Glen A. Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, February 08, 2011 12:17 PM To: Whitaker, Bonnie Cc: Horn, Hazel V; Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High Complexity Testing I should not have included CLIA in my e-mail as it would seem it has clouded things a little. I do apologize. Initially when these issues and guidelines came about CLIA and CAP dovetailed as far as Histology was concerned. Shelia you were looking for contacts that would help you with getting a more solid base to meet these regulations. If you go to the CAP website and click on the IHC link you will find links and publications to assist you. I would recommend that you contact the Applied Immunohistochemistry society as well. NSH or your state/regional society may also have additional information. Should I see something else in my searches I will most willingly forwarded them to you. Vikki On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie wrote: > Hi All, > > There is a difference in performing a task (immunostaining) that is > complex, and performing "high complexity testing" as the CLIA > regulations govern. > > Yes, staining is a complex task, and it requires knowlegable techs to > ensure that it is properly done, and to troubleshoot difficulties > when necessary. > > It is "high complexity testing" because "testing personnel" in > anatomic pathology are pathologists (and the non-physician people > performing gross examinations, who must meet "high complexity testing personnel" > requirements. > > "Testing personnel" as defined by CLIA, are the people that report > results of that test, not people who perform other related duties. > > That's my explanation of the whole mess. > > Bonnie Whitaker > AP Operations Director > Ohio State University Medical Center > Department of Pathology > 614.293.5048 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: Tuesday, February 08, 2011 12:22 PM > To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; > Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > I must disagree with this assessment of what makes a test complex. If > the test is done properly [the responsibility of the technologist] > then the reading to the test is a visual determination that requires > experience on the part of the pathologist, but if the test is not done > properly, will the pathologist be able to tell the technologist what to do to fix the problem? > > Where's the Tylenol? > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Tuesday, February 08, 2011 9:58 AM > To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > While the test is high complexity it is the READING of the test by the > pathologist that determines its complexity. Because histotechs do not > report the results our part of this test is not high complexity. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, February 08, 2011 10:32 AM > To: histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: Re: [Histonet] High Complexity Testing > > When a "machine" is doing the test, there are stringent provisions as > to the preparation and validations of the test. > Done manually, it requires a trained technologists and, yes, they are > high complexity tests (both IHC and FISH, and their variations). > Ren? J. > > --- On Tue, 2/8/11, Sheila Fonner wrote: > > > From: Sheila Fonner > Subject: [Histonet] High Complexity Testing > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 8, 2011, 7:45 AM > > > Hello All, > > > > I would really appreciate it if anyone has information on whether > IHC/ISH are considered high complexity testing for histotechs. Our > pathologist believes that ALL histology low complexity testing since a > "machine" is doing the work. Can anyone help me out with some > guidelines, literature, etc. that says otherwise? I would really > appreciate it. We just want to know which one it is. > > > > Thanks so much Histoland! > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this > message is not the intended recipient, or an employee or agent > responsible for delivering this message to the intended recipient, you > are hereby notified that any dissemination, distribution or copying of > this communication is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to > the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Tue Feb 8 13:49:14 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Tue Feb 8 13:49:42 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: Message-ID: Could not have said it better!!!!! Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper "Dawson, Glen" To Sent by: histonet-bounces@ cc lists.utsouthwest "histonet@lists.utsouthwestern.edu" ern.edu Subject RE: [Histonet] High Complexity 02/08/2011 01:40 Testing PM Alright, if IHC is not high complexity testing, CAP should cut that massive part of their inspection in half and concentrate more on the pathologists' ability to accurately interpret the staining. Too much CAP regulation, Proficiency Testing & validation requirements involved if all IHC is is part of "Processing". My Opinion, Glen A. Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, February 08, 2011 12:17 PM To: Whitaker, Bonnie Cc: Horn, Hazel V; Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High Complexity Testing I should not have included CLIA in my e-mail as it would seem it has clouded things a little. I do apologize. Initially when these issues and guidelines came about CLIA and CAP dovetailed as far as Histology was concerned. Shelia you were looking for contacts that would help you with getting a more solid base to meet these regulations. If you go to the CAP website and click on the IHC link you will find links and publications to assist you. I would recommend that you contact the Applied Immunohistochemistry society as well. NSH or your state/regional society may also have additional information. Should I see something else in my searches I will most willingly forwarded them to you. Vikki On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie wrote: > Hi All, > > There is a difference in performing a task (immunostaining) that is > complex, and performing "high complexity testing" as the CLIA regulations > govern. > > Yes, staining is a complex task, and it requires knowlegable techs to > ensure that it is properly done, and to troubleshoot difficulties when > necessary. > > It is "high complexity testing" because "testing personnel" in anatomic > pathology are pathologists (and the non-physician people performing gross > examinations, who must meet "high complexity testing personnel" > requirements. > > "Testing personnel" as defined by CLIA, are the people that report results > of that test, not people who perform other related duties. > > That's my explanation of the whole mess. > > Bonnie Whitaker > AP Operations Director > Ohio State University Medical Center > Department of Pathology > 614.293.5048 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: Tuesday, February 08, 2011 12:22 PM > To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; > Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > I must disagree with this assessment of what makes a test complex. If the > test is done properly [the responsibility of the technologist] then the > reading to the test is a visual determination that requires experience on > the part of the pathologist, but if the test is not done properly, will the > pathologist be able to tell the technologist what to do to fix the problem? > > Where's the Tylenol? > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Tuesday, February 08, 2011 9:58 AM > To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > While the test is high complexity it is the READING of the test by the > pathologist that determines its complexity. Because histotechs do not > report the results our part of this test is not high complexity. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, February 08, 2011 10:32 AM > To: histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: Re: [Histonet] High Complexity Testing > > When a "machine" is doing the test, there are stringent provisions as to > the preparation and validations of the test. > Done manually, it requires a trained technologists and, yes, they are high > complexity tests (both IHC and FISH, and their variations). > Ren? J. > > --- On Tue, 2/8/11, Sheila Fonner wrote: > > > From: Sheila Fonner > Subject: [Histonet] High Complexity Testing > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 8, 2011, 7:45 AM > > > Hello All, > > > > I would really appreciate it if anyone has information on whether IHC/ISH > are considered high complexity testing for histotechs. Our pathologist > believes that ALL histology low complexity testing since a "machine" is > doing the work. Can anyone help me out with some guidelines, literature, > etc. that says otherwise? I would really appreciate it. We just want to > know which one it is. > > > > Thanks so much Histoland! > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message is > not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it from > your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Elliott <@t> hli.ubc.ca Tue Feb 8 14:52:08 2011 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Tue Feb 8 14:52:55 2011 Subject: [Histonet] IgG3 negative control Message-ID: <4D513C78020000D60004CAC1@mail.mrl.ubc.ca> Can anyone tell me where I can buy mouse IgG3 to use as a negative control for some IHC I am doing. I have looked around in my usual places and can't find anyone who has it. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Jennifer.Hofecker <@t> leica-microsystems.com Tue Feb 8 15:54:09 2011 From: Jennifer.Hofecker <@t> leica-microsystems.com (Jennifer.Hofecker@leica-microsystems.com) Date: Tue Feb 8 15:54:38 2011 Subject: [Histonet] Region III symposium - Call for vendors Message-ID: Hi everyone! The Tennessee Society for Histotechnology will host the Region III symposium in Nashville, TN on April 8-9. If you would like to exhibit at the symposium and need more information, please contact me directly via email or telephone. We offer an unmanned display option for companies that are unable to attend. Deposits are due by March 1 to secure your space. We hope that you will be able to be a part of our symposium. Have a great week! Jennifer Hofecker, HT(ASCP) Tennessee Society for Histotechnology Secretary TSH / Region III Symposium Exhibit Coordinator phone: 800-248-0665 ext 5048 Jennifer.Hofecker@leica-microsystems.com ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From lmayhew5 <@t> cogeco.ca Tue Feb 8 15:54:54 2011 From: lmayhew5 <@t> cogeco.ca (Lee Mayhew) Date: Tue Feb 8 15:55:03 2011 Subject: [Histonet] Cassette labeling Message-ID: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A......When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.....When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada From LSebree <@t> uwhealth.org Tue Feb 8 16:02:26 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Feb 8 16:02:28 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> References: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> Message-ID: <8C023B4AB999614BA4791BAEB26E273839A1C0@UWHC-MAIL01.uwhis.hosp.wisc.edu> B Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A......When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.....When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Feb 8 16:43:44 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Feb 8 16:43:58 2011 Subject: [Histonet] IHC validation Message-ID: <7C7A9E0F28DE4EC3A19B0A8C4E63F4CF@JoePC> Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) From jnocito <@t> satx.rr.com Tue Feb 8 16:52:38 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Feb 8 16:52:54 2011 Subject: [Histonet] High Complexity Testing In-Reply-To: <6B106EE8C8AAEF449DEA97921DEC11670617083E86@EXMBOX-VP05.OSUMC.EDU> References: <000601cbc78e$117561e0$346025a0$@com><329726.98088.qm@web65712.mail.ac4.yahoo.com><25A4DE08332B19499904459F00AAACB7194516A69E@EVS1.archildrens.org> <6B106EE8C8AAEF449DEA97921DEC11670617083E86@EXMBOX-VP05.OSUMC.EDU> Message-ID: <0FA3BF6235A4476B9F6DA233F31BD17F@JoePC> I was devastated to learn that IHC is not considered High Complexity Testing. I agree with Bonnie that we do not report results, therefore, IHC is not considered high complexity testing. Sad but true. Somebody pass the Scotch. I'm way past Tylenol JTT ----- Original Message ----- From: "Whitaker, Bonnie" To: "'Goins, Tresa'" ; "Horn, Hazel V" ; "'Rene J Buesa'" ; ; "Sheila Fonner" Sent: Tuesday, February 08, 2011 11:43 AM Subject: RE: [Histonet] High Complexity Testing Hi All, There is a difference in performing a task (immunostaining) that is complex, and performing "high complexity testing" as the CLIA regulations govern. Yes, staining is a complex task, and it requires knowlegable techs to ensure that it is properly done, and to troubleshoot difficulties when necessary. It is "high complexity testing" because "testing personnel" in anatomic pathology are pathologists (and the non-physician people performing gross examinations, who must meet "high complexity testing personnel" requirements. "Testing personnel" as defined by CLIA, are the people that report results of that test, not people who perform other related duties. That's my explanation of the whole mess. Bonnie Whitaker AP Operations Director Ohio State University Medical Center Department of Pathology 614.293.5048 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Tuesday, February 08, 2011 12:22 PM To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: RE: [Histonet] High Complexity Testing I must disagree with this assessment of what makes a test complex. If the test is done properly [the responsibility of the technologist] then the reading to the test is a visual determination that requires experience on the part of the pathologist, but if the test is not done properly, will the pathologist be able to tell the technologist what to do to fix the problem? Where's the Tylenol? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, February 08, 2011 9:58 AM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: RE: [Histonet] High Complexity Testing While the test is high complexity it is the READING of the test by the pathologist that determines its complexity. Because histotechs do not report the results our part of this test is not high complexity. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 08, 2011 10:32 AM To: histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: Re: [Histonet] High Complexity Testing When a "machine" is doing the test, there are stringent provisions as to the preparation and validations of the test. Done manually, it requires a trained technologists and, yes, they are high complexity tests (both IHC and FISH, and their variations). Ren? J. --- On Tue, 2/8/11, Sheila Fonner wrote: From: Sheila Fonner Subject: [Histonet] High Complexity Testing To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 7:45 AM Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a "machine" is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Feb 8 16:57:40 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Feb 8 16:57:46 2011 Subject: [Histonet] IHC validation In-Reply-To: <7C7A9E0F28DE4EC3A19B0A8C4E63F4CF@JoePC> Message-ID: Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, February 08, 2011 3:44 PM To: Histonet Subject: [Histonet] IHC validation Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Feb 8 17:12:42 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 8 17:12:48 2011 Subject: [Histonet] IHC validation In-Reply-To: References: <7C7A9E0F28DE4EC3A19B0A8C4E63F4CF@JoePC> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A552@CHEXCMS10.one.ads.che.org> But that is for receptors, correct? Do you do that for everything? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 17:58 To: Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, February 08, 2011 3:44 PM To: Histonet Subject: [Histonet] IHC validation Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From liz <@t> premierlab.com Tue Feb 8 17:19:53 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Feb 8 17:19:58 2011 Subject: [Histonet] IHC validation In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A552@CHEXCMS10.one.ads.che.org> Message-ID: Nope that's the recommendation for everything, in the paper they state additional development is required for prognostic markers. Once you have validated an antibody it only requires 3 tissues when you get a new lot number: 1 strong positive, 1 weak to moderate positive and 1 negative. >From how I read the article its 25 tissues and then 3 tissues, it does not talk about slides so it is possible to put multiple tissues on one slide. Again these are just recommendations; I do not think that there is a set standard yet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Tuesday, February 08, 2011 4:13 PM To: Liz Chlipala; Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation But that is for receptors, correct? Do you do that for everything? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 17:58 To: Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, February 08, 2011 3:44 PM To: Histonet Subject: [Histonet] IHC validation Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From CIngles <@t> uwhealth.org Tue Feb 8 17:35:36 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Feb 8 17:35:40 2011 Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI References: <20110208032121.8D2865331B@qmail2.topechelon.com> Message-ID: Sorry Therese, but - Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and have daily contact with the pathologists and many others. Contact me for details if you need to. Bob Merril (if anyone knows him) had this job, but he has retired. Claire UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Therese Cook Sent: Mon 2/7/2011 9:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Slone Partners=eeks a Surgical Pathology Supervisor for our hospital client, located=n Madison, Wisconsin. This key management position, will=versee the staff and the day to day operations of this busy department. =athology Assistants or HT and supervisory experience are required. A BS=s preferred. If you are energetic, have great communication=kills, and a positive attitude, this might be the position for you. Qualified candidates should send their resume to Therese=ook at [1]theresec@slonepartners.com. If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward your=esume to Tara Kochis at [2]tara@slonepartners.com. All inquiries are kept confidential. References 1. 3D"mailto:theresec@slonepartners.com?subject=New%20Candidate%20Request%20%28Surgical%20Pathology%20Supervisor%20-%201455%29" 2. 3D"mailto:tara@slonepartners.com?subject=New%20Candidate%20Request%20"_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Feb 8 17:37:57 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Tue Feb 8 17:38:15 2011 Subject: [Histonet] IHC validation In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A552@CHEXCMS10.one.ads.che.org> Message-ID: When changing instruments you are validating the instrument, not the test. For each antibody you just need to run parallel tests in each instrument showing equivalence. However, if you change the instrument and ALSO change the antibody and/or detection system, Antigen retrieval, etc, then you need to revalidate the test. For most antibodies (Class I FDA exempt, ancillary tests) that involves 5-10 positive and negative cases. A small tissue array can suffice. You should do reproducibility tests for a few antibodies - 5-10 identical slides on one run, 5-10 identical slides on 5-10 runs. When changing Class II antibodies (ER, PR, Her2) and/or their detection systems you will need to run more extensive test. As Liz says, 40 positive, 40 negative. Again, a tissue array is great for that. Changing instruments these days is a big deal when a vendor ties you to their reagents. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 3:20 PM To: Weems, Joyce; Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Nope that's the recommendation for everything, in the paper they state additional development is required for prognostic markers. Once you have validated an antibody it only requires 3 tissues when you get a new lot number: 1 strong positive, 1 weak to moderate positive and 1 negative. >From how I read the article its 25 tissues and then 3 tissues, it does not talk about slides so it is possible to put multiple tissues on one slide. Again these are just recommendations; I do not think that there is a set standard yet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Tuesday, February 08, 2011 4:13 PM To: Liz Chlipala; Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation But that is for receptors, correct? Do you do that for everything? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 17:58 To: Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, February 08, 2011 3:44 PM To: Histonet Subject: [Histonet] IHC validation Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Feb 8 17:46:48 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Feb 8 17:46:51 2011 Subject: [Histonet] IHC validation In-Reply-To: Message-ID: Thanks Tim This is a good way of approaching this. What types of antibodies do you suggest running the reproducibility tests. For reproducibility I run about 3 slides in 3 different runs. I only run reproducibility tests for antibody cross reactivity studies (just after protocol development) and for GLP protocol development. I don't run all of this for my routine protocol development, but we keep tract of everything we have an ongoing log for each antibody and lot number and what conditions they were stained under along with all of the documentation we keep. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Morken, Tim [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, February 08, 2011 4:38 PM To: Liz Chlipala; Weems, Joyce; Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation When changing instruments you are validating the instrument, not the test. For each antibody you just need to run parallel tests in each instrument showing equivalence. However, if you change the instrument and ALSO change the antibody and/or detection system, Antigen retrieval, etc, then you need to revalidate the test. For most antibodies (Class I FDA exempt, ancillary tests) that involves 5-10 positive and negative cases. A small tissue array can suffice. You should do reproducibility tests for a few antibodies - 5-10 identical slides on one run, 5-10 identical slides on 5-10 runs. When changing Class II antibodies (ER, PR, Her2) and/or their detection systems you will need to run more extensive test. As Liz says, 40 positive, 40 negative. Again, a tissue array is great for that. Changing instruments these days is a big deal when a vendor ties you to their reagents. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 3:20 PM To: Weems, Joyce; Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Nope that's the recommendation for everything, in the paper they state additional development is required for prognostic markers. Once you have validated an antibody it only requires 3 tissues when you get a new lot number: 1 strong positive, 1 weak to moderate positive and 1 negative. >From how I read the article its 25 tissues and then 3 tissues, it does not talk about slides so it is possible to put multiple tissues on one slide. Again these are just recommendations; I do not think that there is a set standard yet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Tuesday, February 08, 2011 4:13 PM To: Liz Chlipala; Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation But that is for receptors, correct? Do you do that for everything? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 17:58 To: Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, February 08, 2011 3:44 PM To: Histonet Subject: [Histonet] IHC validation Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amkmadden <@t> gmail.com Tue Feb 8 17:27:27 2011 From: amkmadden <@t> gmail.com (Amanda Madden) Date: Tue Feb 8 17:55:39 2011 Subject: [Histonet] Nickel-DAB Signal Fading Message-ID: Hello Histonetters! I am once again completely baffled, and thought you might be able to help. I run immunocytochemistry on rodent brain tissue every week, always using the exact same procedures, solutions, buffers, chromagen, and mounting medium. The only variable is the primary and secondary antibodies. Our protocol has been time tested and extremely successful. I recently ran a large batch of tissue, and the staining looked great... except that now, about two months later, the signal has faded almost to the point of being gone. I am using Nickel intensified DAB, if that helps, and coverslipping (after an alcohol series finished with xylene) with Permount. Could it be a problem with the primary antibody (one which is new to us but has seemed fine in series dilutions)? Or is it a problem with the chromagen/mounting medium? I have been storing the slides in slides boxes so I don't think it is light. Going crazy, and thankful for any advice, Amanda Madden From jnocito <@t> satx.rr.com Tue Feb 8 18:41:52 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Feb 8 18:42:07 2011 Subject: [Histonet] IHC validation In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A552@CHEXCMS10.one.ads.che.org> Message-ID: <2E94F0C604A049449493EE34F2268D1B@JoePC> fortunately, we don't perform prognostic markers. The doctors don't even want to venture down that road. When I started working here, that's the first ting I mentioned. We can save some much money by doing them in-house. That went over like a lead balloon. On a side note, the staff now were residents when I retired from active duty. Talk about feeling oooooooooooollllllllllllllllllllldddddddddddddd. Thanks guys for the tips. I will pass them on to the powers at be and let them decide. JTT ----- Original Message ----- From: "Morken, Tim" To: "'Liz Chlipala'" ; "Weems, Joyce" ; "Joe Nocito" ; "Histonet" Sent: Tuesday, February 08, 2011 5:37 PM Subject: RE: [Histonet] IHC validation When changing instruments you are validating the instrument, not the test. For each antibody you just need to run parallel tests in each instrument showing equivalence. However, if you change the instrument and ALSO change the antibody and/or detection system, Antigen retrieval, etc, then you need to revalidate the test. For most antibodies (Class I FDA exempt, ancillary tests) that involves 5-10 positive and negative cases. A small tissue array can suffice. You should do reproducibility tests for a few antibodies - 5-10 identical slides on one run, 5-10 identical slides on 5-10 runs. When changing Class II antibodies (ER, PR, Her2) and/or their detection systems you will need to run more extensive test. As Liz says, 40 positive, 40 negative. Again, a tissue array is great for that. Changing instruments these days is a big deal when a vendor ties you to their reagents. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 3:20 PM To: Weems, Joyce; Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Nope that's the recommendation for everything, in the paper they state additional development is required for prognostic markers. Once you have validated an antibody it only requires 3 tissues when you get a new lot number: 1 strong positive, 1 weak to moderate positive and 1 negative. >From how I read the article its 25 tissues and then 3 tissues, it does not talk about slides so it is possible to put multiple tissues on one slide. Again these are just recommendations; I do not think that there is a set standard yet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Tuesday, February 08, 2011 4:13 PM To: Liz Chlipala; Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation But that is for receptors, correct? Do you do that for everything? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 17:58 To: Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, February 08, 2011 3:44 PM To: Histonet Subject: [Histonet] IHC validation Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Tue Feb 8 19:29:23 2011 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Feb 8 19:29:30 2011 Subject: [Histonet] High Complexity Testing References: <000601cbc78e$117561e0$346025a0$@com> <329726.98088.qm@web65712.mail.ac4.yahoo.com> <25A4DE08332B19499904459F00AAACB7194516A69E@EVS1.archildrens.org> <6B106EE8C8AAEF449DEA97921DEC11670617083E86@EXMBOX-VP05.OSUMC.EDU> Message-ID: <90354A475B420441B2A0396E5008D49692BFFB@copc-sbs.COPC.local> Completely agree with you Glen. However, do take exception to the initial comment on this thread about "a machine does all the work". The "machine does all the work" comment is very telling about what this pathologist (or group of pathologists) knows and does not know about running IHC. If the "machine does all the work" what do you need the IHC staff for? To me, that's like saying when a pathologist using image analysis, that the "machine does all the work". Granted you're going to have people with various skill sets and knowledge of histochemical processes, but comments (and attitudes) like that are degrading in nature. If the "machine does all the work" why doesn't this pathologist go to the nearest McDonald's and recruit from they're application files? Just trying to make a point here and tip my hat to everyone working diligently and intelligently even though you may have a "machine doing all the work". Tom Jasper -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, February 08, 2011 10:40 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High Complexity Testing Alright, if IHC is not high complexity testing, CAP should cut that massive part of their inspection in half and concentrate more on the pathologists' ability to accurately interpret the staining. Too much CAP regulation, Proficiency Testing & validation requirements involved if all IHC is is part of "Processing". My Opinion, Glen A. Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, February 08, 2011 12:17 PM To: Whitaker, Bonnie Cc: Horn, Hazel V; Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High Complexity Testing I should not have included CLIA in my e-mail as it would seem it has clouded things a little. I do apologize. Initially when these issues and guidelines came about CLIA and CAP dovetailed as far as Histology was concerned. Shelia you were looking for contacts that would help you with getting a more solid base to meet these regulations. If you go to the CAP website and click on the IHC link you will find links and publications to assist you. I would recommend that you contact the Applied Immunohistochemistry society as well. NSH or your state/regional society may also have additional information. Should I see something else in my searches I will most willingly forwarded them to you. Vikki On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie wrote: > Hi All, > > There is a difference in performing a task (immunostaining) that is > complex, and performing "high complexity testing" as the CLIA regulations > govern. > > Yes, staining is a complex task, and it requires knowlegable techs to > ensure that it is properly done, and to troubleshoot difficulties when > necessary. > > It is "high complexity testing" because "testing personnel" in anatomic > pathology are pathologists (and the non-physician people performing gross > examinations, who must meet "high complexity testing personnel" > requirements. > > "Testing personnel" as defined by CLIA, are the people that report results > of that test, not people who perform other related duties. > > That's my explanation of the whole mess. > > Bonnie Whitaker > AP Operations Director > Ohio State University Medical Center > Department of Pathology > 614.293.5048 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: Tuesday, February 08, 2011 12:22 PM > To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; > Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > I must disagree with this assessment of what makes a test complex. If the > test is done properly [the responsibility of the technologist] then the > reading to the test is a visual determination that requires experience on > the part of the pathologist, but if the test is not done properly, will the > pathologist be able to tell the technologist what to do to fix the problem? > > Where's the Tylenol? > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Tuesday, February 08, 2011 9:58 AM > To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > While the test is high complexity it is the READING of the test by the > pathologist that determines its complexity. Because histotechs do not > report the results our part of this test is not high complexity. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, February 08, 2011 10:32 AM > To: histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: Re: [Histonet] High Complexity Testing > > When a "machine" is doing the test, there are stringent provisions as to > the preparation and validations of the test. > Done manually, it requires a trained technologists and, yes, they are high > complexity tests (both IHC and FISH, and their variations). > Ren? J. > > --- On Tue, 2/8/11, Sheila Fonner wrote: > > > From: Sheila Fonner > Subject: [Histonet] High Complexity Testing > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 8, 2011, 7:45 AM > > > Hello All, > > > > I would really appreciate it if anyone has information on whether IHC/ISH > are considered high complexity testing for histotechs. Our pathologist > believes that ALL histology low complexity testing since a "machine" is > doing the work. Can anyone help me out with some guidelines, literature, > etc. that says otherwise? I would really appreciate it. We just want to > know which one it is. > > > > Thanks so much Histoland! > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message is > not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it from > your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sprice2003 <@t> gmail.com Tue Feb 8 22:37:39 2011 From: sprice2003 <@t> gmail.com (Sally Price) Date: Tue Feb 8 22:37:48 2011 Subject: [Histonet] IHC validation Message-ID: Liz: I'd like to know more about these recommendations. Could you provide a journal reference to the paper from CAP? Thx, Sally ------------------------------ Message: 15 Date: Tue, 8 Feb 2011 16:19:53 -0700 From: "Liz Chlipala" Subject: RE: [Histonet] IHC validation To: "Weems, Joyce" , "Joe Nocito" , "Histonet" histonet@lists.utsouthwestern.edu Nope that's the recommendation for everything, in the paper they state additional development is required for prognostic markers. Once you have validated an antibody it only requires 3 tissues when you get a new lot number: 1 strong positive, 1 weak to moderate positive and 1 negative. >From how I read the article its 25 tissues and then 3 tissues, it does not talk about slides so it is possible to put multiple tissues on one slide. Again these are just recommendations; I do not think that there is a set standard yet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Tuesday, February 08, 2011 4:13 PM To: Liz Chlipala; Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation But that is for receptors, correct? Do you do that for everything? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 17:58 To: Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, February 08, 2011 3:44 PM To: Histonet Subject: [Histonet] IHC validation Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) From wilson6848 <@t> yahoo.com Wed Feb 9 01:06:37 2011 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Wed Feb 9 01:08:28 2011 Subject: [Histonet] Fw: Looking for position in Texas Message-ID: <3420.10692.qm@web120912.mail.ne1.yahoo.com> ? ??? Hello all, ???????????????? I am looking for a new histotech position in texas, especially in the houston area?so as to be, ?very?close to my family. Though, any part of texas is okay. ??? I am an HT(ASCP) certified histotech. I do have substancial years of experience and with QIHC(ASCP) ???????? Any leads will be greatly appreciated. ??? Wilson. ____________________________________________________________________________________ Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. http://tools.search.yahoo.com/toolbar/features/mail/ From Nele.Degryse <@t> UGent.be Wed Feb 9 02:30:44 2011 From: Nele.Degryse <@t> UGent.be (Nele Degryse) Date: Wed Feb 9 02:30:58 2011 Subject: [Histonet] Tape transfer Message-ID: <20110209093044.19282fuvp27e2ugk@webmail.ugent.be> Hello, I'm trying to cut undecalcified bone (mouse knees) by using the tape transfer method. The problem is that the area that I'm interested in (the joint with patella) is not transferred from the tape on my slide. I've used slides with different coating (also those specially made for bone) and tried different thickness. None of that seems to make a difference. Another problem is that my hand roller started to stick, so I cleaned it with 100% ethanol (as recommended) and now it sticks even more... Can anybody give me some advice? Thanks From An.Eerdekens <@t> med.kuleuven.be Wed Feb 9 04:52:35 2011 From: An.Eerdekens <@t> med.kuleuven.be (An Eerdekens) Date: Wed Feb 9 04:52:48 2011 Subject: [Histonet] failed thionin staining part 2 Message-ID: <85BD21DE1A6DE94D9A30A2F2C1D2328B3D5641C2B9@ICTS-S-EXC4-CA.luna.kuleuven.be> Dear friends of Histonet, I have send already a message last week considering problems with thionin staining. I got a lot of protocols, thank you for that. We used different kind of solutions, but the problem remains, independently of the solution: when our samples go through the ethanol (50%, 70%, 90%, 100%) the thionin is completely washed of. Has anyone got the same experience? What to do about it? regards, An Eerdekens From esarricks <@t> gmail.com Wed Feb 9 06:38:13 2011 From: esarricks <@t> gmail.com (Erin Sarricks) Date: Wed Feb 9 06:38:19 2011 Subject: [Histonet] PAS with Diastase Digestion Message-ID: Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both "with" and "without" slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1. Deparaffinize and hydrate to water. 2. Place the sections labeled ?with? in diastase solution preheated to 37?C for 1 hour. Hold the sections labeled ?without? in distilled water. 3. Wash in running water for 5 minutes 4. Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5. Wash in 3 changes of distilled water 6. Place in Schiff reagent for 15 minutes 7. Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8. Counterstain in Mayer?s Hematoxylin for 3 minutes. 9. Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarricks@us.army.mil From LSebree <@t> uwhealth.org Wed Feb 9 07:35:07 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Feb 9 07:35:11 2011 Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI In-Reply-To: References: <20110208032121.8D2865331B@qmail2.topechelon.com> Message-ID: <8C023B4AB999614BA4791BAEB26E273839A1C1@UWHC-MAIL01.uwhis.hosp.wisc.edu> You're not kidding Claire! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, February 08, 2011 5:36 PM To: Therese Cook; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Sorry Therese, but - Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and have daily contact with the pathologists and many others. Contact me for details if you need to. Bob Merril (if anyone knows him) had this job, but he has retired. Claire UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Therese Cook Sent: Mon 2/7/2011 9:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Slone Partners=eeks a Surgical Pathology Supervisor for our hospital client, located=n Madison, Wisconsin. This key management position, will=versee the staff and the day to day operations of this busy department. =athology Assistants or HT and supervisory experience are required. A BS=s preferred. If you are energetic, have great communication=kills, and a positive attitude, this might be the position for you. Qualified candidates should send their resume to Therese=ook at [1]theresec@slonepartners.com. If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward your=esume to Tara Kochis at [2]tara@slonepartners.com. All inquiries are kept confidential. References 1. 3D"mailto:theresec@slonepartners.com?subject=New%20Candidate%20Request%2 0%28Surgical%20Pathology%20Supervisor%20-%201455%29" 2. 3D"mailto:tara@slonepartners.com?subject=New%20Candidate%20Request%20"__ _____________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Feb 9 07:52:14 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Feb 9 07:52:47 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> Message-ID: A -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 4:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A......When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.....When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Wanda.Smith <@t> HCAhealthcare.com Wed Feb 9 07:54:59 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Feb 9 07:55:06 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <8C023B4AB999614BA4791BAEB26E273839A1C0@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> <8C023B4AB999614BA4791BAEB26E273839A1C0@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139ED53117@NADCWPMSGCMS03.hca.corpad.net> B WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Tuesday, February 08, 2011 5:02 PM To: Lee Mayhew; Histonet Subject: RE: [Histonet] Cassette labeling B Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A......When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.....When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Wed Feb 9 08:03:46 2011 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Feb 9 08:04:10 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <8C023B4AB999614BA4791BAEB26E273839A1C0@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> <8C023B4AB999614BA4791BAEB26E273839A1C0@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: B Then when I file the blocks the number is right side up Roberta Horner Animal Diagnostic Lab Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A......When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.....When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Wed Feb 9 08:30:35 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Feb 9 08:30:48 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> Message-ID: B. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Lee Mayhew Sent by: histonet-bounces@lists.utsouthwestern.edu 02/08/2011 04:57 PM To Histonet cc Subject [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A......When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.....When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From cbrya <@t> lexclin.com Wed Feb 9 08:44:50 2011 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Wed Feb 9 08:44:56 2011 Subject: [Histonet] cassette slide/labeling Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BA2D6B21@EXCHANGESB> We are looking to get a bar-coded system in our laboratory. We currently hand write all cassettes and slides. What labelers do you have and do you like them? Also does anyone have the Ventana Vantage system? If so, would you recommend Vantage? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From TMcNemar <@t> lmhealth.org Wed Feb 9 09:04:31 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Feb 9 09:04:38 2011 Subject: [Histonet] PAS with Diastase Digestion In-Reply-To: References: Message-ID: Spit for 5 minutes.... old school.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both "with" and "without" slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1. Deparaffinize and hydrate to water. 2. Place the sections labeled ?with? in diastase solution preheated to 37?C for 1 hour. Hold the sections labeled ?without? in distilled water. 3. Wash in running water for 5 minutes 4. Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5. Wash in 3 changes of distilled water 6. Place in Schiff reagent for 15 minutes 7. Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8. Counterstain in Mayer?s Hematoxylin for 3 minutes. 9. Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarricks@us.army.mil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From flnails <@t> texaschildrens.org Wed Feb 9 09:24:36 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Feb 9 09:24:56 2011 Subject: [Histonet] Mouse staining for ATP In-Reply-To: References: Message-ID: I am looking for a reliable method for staining mouse muscle with ATP'ase pH 4.3, 4.6 and 9.4. Any help will be greatly appreciated. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, February 09, 2011 9:05 AM To: 'Erin Sarricks'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PAS with Diastase Digestion Spit for 5 minutes.... old school.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both "with" and "without" slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1. Deparaffinize and hydrate to water. 2. Place the sections labeled ?with? in diastase solution preheated to 37?C for 1 hour. Hold the sections labeled ?without? in distilled water. 3. Wash in running water for 5 minutes 4. Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5. Wash in 3 changes of distilled water 6. Place in Schiff reagent for 15 minutes 7. Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8. Counterstain in Mayer?s Hematoxylin for 3 minutes. 9. Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarricks@us.army.mil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From sfonner <@t> labpath.com Wed Feb 9 09:39:13 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Wed Feb 9 09:41:51 2011 Subject: [Histonet] High Complexity Testing Message-ID: <000a01cbc86f$80cc9f90$8265deb0$@com> I would just like to thank everyone for all of your comments regarding the high complexity testing issue. I obviously opened a can of worms on that one! I think I understand it a little better now as I have come to the following conclusion: Although CAP requires strict guidelines and documentation regarding optimization, validation, controls, lot testing and storage (all of which are done by a tech.), the end result is the reading of the slide by a pathologist, who gets the credit for the high complexity testing. All of our hard work that provides the slide to him/her is considered a high complexity "task" or specimen processing. If any of you are asked this question in the future I think you will all have a better understanding of how to answer. I'm most grateful for the input. Thanks again. Sheila From Marcia_Gaiser <@t> ssmhc.com Wed Feb 9 09:55:00 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Wed Feb 9 09:55:43 2011 Subject: [Histonet] HT Postion Oklahoma City Message-ID: <728F817C02110E498D803A7C3B0C6248057D85BD50@S009-APEXM06.ds.ad.ssmhc.com> St. Anthony Hospital currently has an excellent opportunity for an experienced Histologic Technician. This position requires Certification as an HT or HLT ? or ? experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.com, Ad # 10762, or contact Anna King for additional information at (405) 272-6105. Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From sgoebel <@t> mirnarx.com Wed Feb 9 10:11:24 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Feb 9 10:11:27 2011 Subject: [Histonet] Caspase 3 Message-ID: I know I have asked about this in the past, but I'm finally getting around to optimizing my antibody (Caspase3). First, tonsil will be a good control? Second, I ordered it from Abcam that is telling me the optimal dilution is between 1/10 and 1/20. This seems a little crazy? I looked at some reviews for the antibody and other people seem to have used this dilution...wow! This is going to be an expensive antibody to use!!! Anyhoo, main thing is want to make sure that tonsil will be ok for a positive control =) Stay warm my fellow Texans...it'll be 80 on Sunday!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From CCole <@t> sjha.org Wed Feb 9 10:24:35 2011 From: CCole <@t> sjha.org (Cole, Caryn) Date: Wed Feb 9 10:24:42 2011 Subject: [Histonet] Histology Opening in Atlanta, GA Message-ID: Hello, my name is Caryn Cole, I am a recruiter with St Joseph's Hospital in Atlanta GA and I have an opening for a Histology Technician at our Research Institute. I have listed the job description and required qualifications about the position below. If you are not interested please feel free to pass this email along to anyone who may like to apply for this position. Job Details: Saint Joseph's Hospital of Atlanta is committed to translational research through activities in the Saint Joseph's Translational Research Institute (SJTRI). Saint Joseph's Translational Research Institute is known as a leader in the treatment of cardiac and vascular diseases, orthopedics, cancer, gastroenterology, and diabetes. SJTRI has gathered scientists, industry partners, recognized physicians, and a collaborative network of national leaders in the evolving arena of translational research. Integrating top research scientists, sophisticated research facilities, education and training, SJTRI is engaged in bold and exciting programs designed to assist in the investigation of cutting edge treatments with potential benefits to patients and the community. The Histology Tech obtains, identifies and prepares tissue at necropsy for histological processing and assists in histological processing of tissue samples. Also implements SJTRI's OSHA safety program. The ideal candidate will have an Associate degree from accredited college or university in Biology or related biomedical subject or equivalent work experience. ASCP Certification within 1 year of hire. Formal safety training by OSHA certified Safety Instructor. Two years histology experience with paraffin embedding and frozen tissue immuno-histochemistry. One year experience supporting corporate safety policies. Some MS Windows experience. Full time position. Shift: 8:30a-5:00p If interested please go to www.stjosephsatlanta.org and apply. Regards, Caryn Cole Employment Specialist St Joseph's Hospital Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mtitford <@t> aol.com Wed Feb 9 10:34:44 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Feb 9 10:34:59 2011 Subject: [Histonet] Thionin stain Message-ID: <8CD968E6442E2AD-1C54-4909@webmail-d070.sysops.aol.com> An Eerdekens overseas somewhere asks about thionin staining: An, you may have a bad batch of thionin stain. Did you just open a new jar? Here is the USA we use stains certified for use in histology by the Biological Stain Commission. Each lot has been tested by them for use in histology. Maybe you could borrow some thionin from another lab and see if that works better for you? Sometimes in my career I have come across bad jars of stain that just did not work. Michael Titford Pathology USA Mobile AL From theresec <@t> slonepartners.com Wed Feb 9 10:37:27 2011 From: theresec <@t> slonepartners.com (Therese Cook) Date: Wed Feb 9 10:37:34 2011 Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI In-Reply-To: References: <20110208032121.8D2865331B@qmail2.topechelon.com> Message-ID: <996734E2-2E05-4419-8E94-2A692983B88E@slonepartners.com> Hi Claire and all: I think you are confusing this position with a different position in Madison. The supervisor in this position will oversee Histology including IHC and the gross room. There are no autopsies and no renal lab. The ideal candidate will have experience with LEAN or workflow redesign. I look forward to speaking with anyone who is qualified and would be happy to give you more details. Regards, Therese Slone Partners On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote: > Sorry Therese, but - > Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and have daily contact with the pathologists and many others. Contact me for details if you need to. Bob Merril (if anyone knows him) had this job, but he has retired. > Claire > UW Hospital and Clinics > Madison WI > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Therese Cook > Sent: Mon 2/7/2011 9:21 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI > > > > > Slone Partners=eeks a Surgical Pathology Supervisor for our hospital > client, located=n Madison, Wisconsin. > > > > This key management position, will=versee the staff and the day > to day operations of this busy department. =athology Assistants or > HT and supervisory experience are required. A BS=s preferred. > > > > If you are energetic, have great communication=kills, and a > positive attitude, this might be the position for you. > > > > Qualified candidates should send their resume to Therese=ook at > [1]theresec@slonepartners.com. > > > > If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward > your=esume to Tara Kochis at [2]tara@slonepartners.com. > > > > All inquiries are kept confidential. > > References > > 1. 3D"mailto:theresec@slonepartners.com?subject=New%20Candidate%20Request%20%28Surgical%20Pathology%20Supervisor%20-%201455%29" > 2. 3D"mailto:tara@slonepartners.com?subject=New%20Candidate%20Request%20"_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX: 330.232.9333 www.slonepartners.com -------------- next part -------------- From sgoebel <@t> mirnarx.com Wed Feb 9 10:38:08 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Feb 9 10:38:12 2011 Subject: [Histonet] One more thing...I feel like Columbo Message-ID: So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From mjdessoye <@t> wvhcs.org Wed Feb 9 10:52:23 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Wed Feb 9 10:52:30 2011 Subject: [Histonet] Benchmark Problems Message-ID: Hello, We started having a strange problem with our Ventana Benchmark XT. Out of the blue, our slides stopped staining. With most antibodies we get no reaction, some that were very strongly positive are now only very lightly staining. They do appear to counterstain OK. Following Ventana's recommendations we completely changed out all of our bulk fluids, purged the lines, changed antibodies and DAB kits. The last shot is some kind of instrument issue that's not triggering an error. Anyone ever see anything like this? Mike Dessoye _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From marktarango <@t> gmail.com Wed Feb 9 10:58:41 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Feb 9 10:58:51 2011 Subject: [Histonet] Benchmark Problems In-Reply-To: References: Message-ID: Hi Mike, Did you wash the bottles and rinse them and then pump the water through the instrument before adding reagents and purging all again? We had a problem like this about 2 weeks ago. I think someone loaded the wrong bulk reagent onto the instrument. Cleaning it, purging with water and adding newly made bulk reagents fixed the problem for us. Mark On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J wrote: > Hello, > > We started having a strange problem with our Ventana Benchmark XT. Out of > the blue, our slides stopped staining. With most antibodies we get no > reaction, some that were very strongly positive are now only very lightly > staining. They do appear to counterstain OK. Following Ventana's > recommendations we completely changed out all of our bulk fluids, purged the > lines, changed antibodies and DAB kits. The last shot is some kind of > instrument issue that's not triggering an error. Anyone ever see anything > like this? > > Mike Dessoye > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Wyoming Valley Health Care System. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wbenton <@t> cua.md Wed Feb 9 11:13:21 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Feb 9 11:14:16 2011 Subject: [Histonet] RE: One more thing...I feel like Columbo In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD91172BF187F@CUAEXH1.GCU-MD.local> Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com [sgoebel@mirnarx.com] Sent: Wednesday, February 09, 2011 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From wbenton <@t> cua.md Wed Feb 9 11:14:52 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Feb 9 11:15:19 2011 Subject: [Histonet] RE: One more thing...I feel like Columbo In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD91172BF1880@CUAEXH1.GCU-MD.local> https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=L10831&categoryId=81937&productId=12706060 http://www.medite.de/ebp50.html?&L=1 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com [sgoebel@mirnarx.com] Sent: Wednesday, February 09, 2011 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From hymclab.hymclab <@t> ministryhealth.org Wed Feb 9 11:15:26 2011 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Wed Feb 9 11:15:40 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> References: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> Message-ID: "A" For filing reasons. When we file in the boxes on top of microtome they are right side up and for final filing in the plastic drawers writing is right side up. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A......When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.....When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From JWeems <@t> sjha.org Wed Feb 9 11:19:34 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Feb 9 11:19:38 2011 Subject: [Histonet] RE: One more thing...I feel like Columbo In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A659@CHEXCMS10.one.ads.che.org> I think you can get from Thermo Scientific. Two sites found on line..j http://www.medite.de/ebp50.html?&L=1 https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=L10831&categoryId=81937&productId=12706060 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, February 09, 2011 11:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From b-frederick <@t> northwestern.edu Wed Feb 9 11:24:41 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Feb 9 11:24:53 2011 Subject: [Histonet] Cassette labeling In-Reply-To: Message-ID: <4A645070A2B746CA9B569BAA6DA6FDBC@lurie.northwestern.edu> B Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, February 09, 2011 11:15 AM To: 'Lee Mayhew'; Histonet Subject: RE: [Histonet] Cassette labeling "A" For filing reasons. When we file in the boxes on top of microtome they are right side up and for final filing in the plastic drawers writing is right side up. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A......When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.....When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Feb 9 11:27:08 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Feb 9 11:27:35 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <4A645070A2B746CA9B569BAA6DA6FDBC@lurie.northwestern.edu> References: <4A645070A2B746CA9B569BAA6DA6FDBC@lurie.northwestern.edu> Message-ID: <4D52CE6C.2030908@pathology.washington.edu> For those of you that answered with B, how are you filing the blocks? Just curious. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/9/2011 9:24 AM, Bernice Frederick wrote: > B > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab > Sent: Wednesday, February 09, 2011 11:15 AM > To: 'Lee Mayhew'; Histonet > Subject: RE: [Histonet] Cassette labeling > > "A" For filing reasons. When we file in the boxes on top of microtome they > are right side up and for final filing in the plastic drawers writing is > right side up. > > Dawn > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew > Sent: Tuesday, February 08, 2011 3:55 PM > To: Histonet > Subject: [Histonet] Cassette labeling > > Hi Histonetters, > > At my hospital, we are having a discussion about how to label cassettes. I > have worked at 2 hospitals, and they each do it a different way. Our > cassette labeller will print either way. > > Could you please indicate which way you do it at your site, A or B. > > A......When the cassette is open and sitting on the bench facing you > with the lid on the far side and the surface for writing on is closest to > you, the surgical number is upside down. > > B.....When the cassette is open and sitting on the bench facing you > with the lid on the far side and the surface for writing on is closest to > you, the surgical number is right side up. > > Thanks in advance. > > Lee Mayhew MLT > St. Josephs Hospital > Hamilton, ON Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the > designated recipient(s) named above. If you are not the intended recipient, > you are hereby notified that you have received this communication in error > and that any review, disclosure, dissemination, distribution or copying of > it or its contents is prohibited. If you have received this communication in > error, please notify the sender at the electronic mail address noted above > and destroy all copies of this communication and any attachments. Thank you > for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Wed Feb 9 11:30:27 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Feb 9 11:30:34 2011 Subject: [Histonet] RE: cassette slide/labeling In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BA2D6B21@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BA2D6B21@EXCHANGESB> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7347@EXCHMBC2.ad.ah.local> I would highly recommend the Vantage system. We have had it in place for over 2 years and our accuracy rate went from about 94-94% to over 99.9%. We have had only 2 numbering errors in Histo in over 2 years.(due to people not following procedure correctly) The system does so much more that tracking. The statistical reports are wonderful. Please contact me off line if you want to know details. Jan Mahoney 402-717-2889 Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Wednesday, February 09, 2011 8:45 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette slide/labeling We are looking to get a bar-coded system in our laboratory. We currently hand write all cassettes and slides. What labelers do you have and do you like them? Also does anyone have the Ventana Vantage system? If so, would you recommend Vantage? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From JWeems <@t> sjha.org Wed Feb 9 11:48:54 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Feb 9 11:48:58 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <4D52CE6C.2030908@pathology.washington.edu> References: <4A645070A2B746CA9B569BAA6DA6FDBC@lurie.northwestern.edu> <4D52CE6C.2030908@pathology.washington.edu> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A66E@CHEXCMS10.one.ads.che.org> I think they must stand on their head backwards....j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, February 09, 2011 12:27 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette labeling For those of you that answered with B, how are you filing the blocks? Just curious. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/9/2011 9:24 AM, Bernice Frederick wrote: > B > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > hymclab > Sent: Wednesday, February 09, 2011 11:15 AM > To: 'Lee Mayhew'; Histonet > Subject: RE: [Histonet] Cassette labeling > > "A" For filing reasons. When we file in the boxes on top of microtome > they are right side up and for final filing in the plastic drawers > writing is right side up. > > Dawn > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee > Mayhew > Sent: Tuesday, February 08, 2011 3:55 PM > To: Histonet > Subject: [Histonet] Cassette labeling > > Hi Histonetters, > > At my hospital, we are having a discussion about how to label > cassettes. I have worked at 2 hospitals, and they each do it a > different way. Our cassette labeller will print either way. > > Could you please indicate which way you do it at your site, A or B. > > A......When the cassette is open and sitting on the bench facing > you with the lid on the far side and the surface for writing on is > closest to you, the surgical number is upside down. > > B.....When the cassette is open and sitting on the bench facing > you with the lid on the far side and the surface for writing on is > closest to you, the surgical number is right side up. > > Thanks in advance. > > Lee Mayhew MLT > St. Josephs Hospital > Hamilton, ON Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments > may contain confidential and privileged information for the use of the > designated recipient(s) named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please notify the sender at > the electronic mail address noted above and destroy all copies of this > communication and any attachments. Thank you for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From LSebree <@t> uwhealth.org Wed Feb 9 12:01:51 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Feb 9 12:01:56 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A66E@CHEXCMS10.one.ads.che.org> References: <4A645070A2B746CA9B569BAA6DA6FDBC@lurie.northwestern.edu><4D52CE6C.2030908@pathology.washington.edu> <92AD9B20A6C38C4587A9FEBE3A30E164081E09A66E@CHEXCMS10.one.ads.che.org> Message-ID: <8C023B4AB999614BA4791BAEB26E273839A1C5@UWHC-MAIL01.uwhis.hosp.wisc.edu> Using the "B" method ends up with the case # right side up in the block file....unless I completely misunderstood the orientations of "A" and "B"; entirely possible! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, February 09, 2011 11:49 AM To: Victor Tobias; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette labeling I think they must stand on their head backwards....j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, February 09, 2011 12:27 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette labeling For those of you that answered with B, how are you filing the blocks? Just curious. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/9/2011 9:24 AM, Bernice Frederick wrote: > B > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > hymclab > Sent: Wednesday, February 09, 2011 11:15 AM > To: 'Lee Mayhew'; Histonet > Subject: RE: [Histonet] Cassette labeling > > "A" For filing reasons. When we file in the boxes on top of microtome > they are right side up and for final filing in the plastic drawers > writing is right side up. > > Dawn > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee > Mayhew > Sent: Tuesday, February 08, 2011 3:55 PM > To: Histonet > Subject: [Histonet] Cassette labeling > > Hi Histonetters, > > At my hospital, we are having a discussion about how to label > cassettes. I have worked at 2 hospitals, and they each do it a > different way. Our cassette labeller will print either way. > > Could you please indicate which way you do it at your site, A or B. > > A......When the cassette is open and sitting on the bench facing > you with the lid on the far side and the surface for writing on is > closest to you, the surgical number is upside down. > > B.....When the cassette is open and sitting on the bench facing > you with the lid on the far side and the surface for writing on is > closest to you, the surgical number is right side up. > > Thanks in advance. > > Lee Mayhew MLT > St. Josephs Hospital > Hamilton, ON Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments > may contain confidential and privileged information for the use of the > designated recipient(s) named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please notify the sender at > the electronic mail address noted above and destroy all copies of this > communication and any attachments. Thank you for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brian <@t> prometheushealthcare.com Wed Feb 9 12:02:02 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Wed Feb 9 12:02:14 2011 Subject: [Histonet] Part/Full time Histology Openings in Suffern NY Message-ID: <016901cbc883$77918e90$66b4abb0$@com> Growing private lab searching for the following openings: IHC Specialist (am) Lead Histotech (8-4 T-S) Histotechs for am T-S & 3-11 M-f Excellent pay - the highest out of any company we work with in the tri-state area! Part -time openings also available. Please contact me today for immediate consideration. Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From Jenee.S.Odani <@t> hawaii.gov Wed Feb 9 12:10:55 2011 From: Jenee.S.Odani <@t> hawaii.gov (Jenee.S.Odani@hawaii.gov) Date: Wed Feb 9 12:11:01 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <8C023B4AB999614BA4791BAEB26E273839A1C5@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: We've used both systems here. Both can produce right side up filing, depending on which way the wax faces (i.e. towards you, or away from you). Because we hand label the cassettes here, I prefer to use A, because the label bevel is at the top and easier to hold that way. Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 "Sebree Linda A" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/09/2011 08:03 AM To "Weems, Joyce" , "Victor Tobias" , cc Subject RE: [Histonet] Cassette labeling Using the "B" method ends up with the case # right side up in the block file....unless I completely misunderstood the orientations of "A" and "B"; entirely possible! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, February 09, 2011 11:49 AM To: Victor Tobias; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette labeling I think they must stand on their head backwards....j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, February 09, 2011 12:27 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette labeling For those of you that answered with B, how are you filing the blocks? Just curious. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/9/2011 9:24 AM, Bernice Frederick wrote: > B > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > hymclab > Sent: Wednesday, February 09, 2011 11:15 AM > To: 'Lee Mayhew'; Histonet > Subject: RE: [Histonet] Cassette labeling > > "A" For filing reasons. When we file in the boxes on top of microtome > they are right side up and for final filing in the plastic drawers > writing is right side up. > > Dawn > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee > Mayhew > Sent: Tuesday, February 08, 2011 3:55 PM > To: Histonet > Subject: [Histonet] Cassette labeling > > Hi Histonetters, > > At my hospital, we are having a discussion about how to label > cassettes. I have worked at 2 hospitals, and they each do it a > different way. Our cassette labeller will print either way. > > Could you please indicate which way you do it at your site, A or B. > > A......When the cassette is open and sitting on the bench facing > you with the lid on the far side and the surface for writing on is > closest to you, the surgical number is upside down. > > B.....When the cassette is open and sitting on the bench facing > you with the lid on the far side and the surface for writing on is > closest to you, the surgical number is right side up. > > Thanks in advance. > > Lee Mayhew MLT > St. Josephs Hospital > Hamilton, ON Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments > may contain confidential and privileged information for the use of the > designated recipient(s) named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please notify the sender at > the electronic mail address noted above and destroy all copies of this > communication and any attachments. Thank you for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Feb 9 12:11:28 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Feb 9 12:11:32 2011 Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI In-Reply-To: <996734E2-2E05-4419-8E94-2A692983B88E@slonepartners.com> References: <20110208032121.8D2865331B@qmail2.topechelon.com> <996734E2-2E05-4419-8E94-2A692983B88E@slonepartners.com> Message-ID: <8C023B4AB999614BA4791BAEB26E273839A1C7@UWHC-MAIL01.uwhis.hosp.wisc.edu> Claire and I were thinking this was an advertisement for the University of Wisconsin Hospital and Clinics Surgical Pathology manager position. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therese Cook Sent: Wednesday, February 09, 2011 10:37 AM To: Ingles Claire Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Hi Claire and all: I think you are confusing this position with a different position in Madison. The supervisor in this position will oversee Histology including IHC and the gross room. There are no autopsies and no renal lab. The ideal candidate will have experience with LEAN or workflow redesign. I look forward to speaking with anyone who is qualified and would be happy to give you more details. Regards, Therese Slone Partners On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote: > Sorry Therese, but - > Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and have daily contact with the pathologists and many others. Contact me for details if you need to. Bob Merril (if anyone knows him) had this job, but he has retired. > Claire > UW Hospital and Clinics > Madison WI > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Therese Cook > Sent: Mon 2/7/2011 9:21 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI > > > > > Slone Partners=eeks a Surgical Pathology Supervisor for our hospital > client, located=n Madison, Wisconsin. > > > > This key management position, will=versee the staff and the day > to day operations of this busy department. =athology Assistants or > HT and supervisory experience are required. A BS=s preferred. > > > > If you are energetic, have great communication=kills, and a > positive attitude, this might be the position for you. > > > > Qualified candidates should send their resume to Therese=ook at > [1]theresec@slonepartners.com. > > > > If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward > your=esume to Tara Kochis at [2]tara@slonepartners.com. > > > > All inquiries are kept confidential. > > References > > 1. 3D"mailto:theresec@slonepartners.com?subject=New%20Candidate%20Request%2 0%28Surgical%20Pathology%20Supervisor%20-%201455%29" > 2. 3D"mailto:tara@slonepartners.com?subject=New%20Candidate%20Request%20"__ _____________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX: 330.232.9333 www.slonepartners.com From Rcartun <@t> harthosp.org Wed Feb 9 12:12:05 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Feb 9 12:12:12 2011 Subject: [Histonet] CPT code 88363 Message-ID: <4D5292A4.7400.0077.1@harthosp.org> Is anyone using the above CPT code for reviewing "old" pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From tkngflght <@t> yahoo.com Wed Feb 9 12:19:32 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Feb 9 12:19:35 2011 Subject: [Histonet] two temps needed - day shift Message-ID: <750771.67187.qm@web39410.mail.mud.yahoo.com> Hello all- ? We're seeking two experienced travel temps...both situations are day shift, for up to 8 weeks.? Please send resume for return call- ? admin@fullstaff.org fax 800.756.3309 ? Permanent positions also available (perm, temp or temp-to-perm depending on related experience and a few other factors) ? Thanks for your interest! ? ? From Ashley.Troutman <@t> Vanderbilt.Edu Wed Feb 9 12:23:29 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Wed Feb 9 12:23:34 2011 Subject: [Histonet] IHC validation Message-ID: <7B310892042DA74CB3590053F424CFE61387EF185E@ITS-HCWNEM06.ds.Vanderbilt.edu> I believe the 25 cases are for ER/PR/Her-2 testing. We have done this recently and we ran about 10 for each antibody. We have over 100 ourselves and it took quite a while. There will be some antibodies that you will be unable to find 10 slides to test, so do as many (or as few) as your Medical Director is comfortable with to validate the stain. Good luck! Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 14 Date: Tue, 8 Feb 2011 18:12:42 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] IHC validation To: Liz Chlipala , Joe Nocito , Histonet Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A552@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" But that is for receptors, correct? Do you do that for everything? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 17:58 To: Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, February 08, 2011 3:44 PM To: Histonet Subject: [Histonet] IHC validation Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) From theresec <@t> slonepartners.com Wed Feb 9 12:25:27 2011 From: theresec <@t> slonepartners.com (Therese Cook) Date: Wed Feb 9 12:25:35 2011 Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI In-Reply-To: <8C023B4AB999614BA4791BAEB26E273839A1C7@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <20110208032121.8D2865331B@qmail2.topechelon.com> <996734E2-2E05-4419-8E94-2A692983B88E@slonepartners.com> <8C023B4AB999614BA4791BAEB26E273839A1C7@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <79CAEF57-38AE-43BC-8440-99D6BF7D2771@slonepartners.com> Right. It is NOT with the University of Wisconsin. On Feb 9, 2011, at 1:11 PM, Sebree Linda A wrote: > Claire and I were thinking this was an advertisement for the University > of Wisconsin Hospital and Clinics Surgical Pathology manager position. > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therese > Cook > Sent: Wednesday, February 09, 2011 10:37 AM > To: Ingles Claire > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Seeking a Surgical Pathology Supervisor in > Madison, WI > > Hi Claire and all: > > I think you are confusing this position with a different position in > Madison. The supervisor in this position will oversee Histology > including IHC and the gross room. There are no autopsies and no renal > lab. The ideal candidate will have experience with LEAN or workflow > redesign. I look forward to speaking with anyone who is qualified and > would be happy to give you more details. > > Regards, > > Therese > Slone Partners > > On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote: > >> Sorry Therese, but - >> Only consider this position if you like to be Really busy. As I > respect all on this list. I will issue a warning that this position is > much more involved than the 'ad' says. You would be overseeing the Histo > lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and > have daily contact with the pathologists and many others. Contact me for > details if you need to. Bob Merril (if anyone knows him) had this job, > but he has retired. >> Claire >> UW Hospital and Clinics >> Madison WI >> >> ________________________________ >> >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Therese > Cook >> Sent: Mon 2/7/2011 9:21 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Seeking a Surgical Pathology Supervisor in > Madison, WI >> >> >> >> >> Slone Partners=eeks a Surgical Pathology Supervisor for our hospital >> client, located=n Madison, Wisconsin. >> >> >> >> This key management position, will=versee the staff and the day >> to day operations of this busy department. =athology Assistants or >> HT and supervisory experience are required. A BS=s preferred. >> >> >> >> If you are energetic, have great communication=kills, and a >> positive attitude, this might be the position for you. >> >> >> >> Qualified candidates should send their resume to Therese=ook at >> [1]theresec@slonepartners.com. >> >> >> >> If you do not meet these qualifications, but wish to be considered > for other roles in the laboratory diagnostic industry, please forward >> your=esume to Tara Kochis at [2]tara@slonepartners.com. >> >> >> >> All inquiries are kept confidential. >> >> References >> >> 1. > 3D"mailto:theresec@slonepartners.com?subject=New%20Candidate%20Request%2 > 0%28Surgical%20Pathology%20Supervisor%20-%201455%29" >> 2. > 3D"mailto:tara@slonepartners.com?subject=New%20Candidate%20Request%20"__ > _____________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > SLONEPARTNERS > THERESE COOK - EXECUTIVE RECRUITER > > Corporate Headquarters > 1521 Alton Road #638 > Miami Beach, Florida 33139 > > TOLL FREE: 877.419.1439 > DIRECT: 330.863.1054 > CELL: 330.323.4525 > FAX: 330.232.9333 > > www.slonepartners.com > > > SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX: 330.232.9333 www.slonepartners.com -------------- next part -------------- From JWeems <@t> sjha.org Wed Feb 9 12:27:29 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Feb 9 12:27:33 2011 Subject: [Histonet] CPT code 88363 In-Reply-To: <4D5292A4.7400.0077.1@harthosp.org> References: <4D5292A4.7400.0077.1@harthosp.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A696@CHEXCMS10.one.ads.che.org> Only molecular... And I read that somewhere, but don't remember where. I'll search. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, February 09, 2011 13:12 To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing "old" pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Debbie.Lake <@t> mgh.net Wed Feb 9 12:34:27 2011 From: Debbie.Lake <@t> mgh.net (Lake,Debbie) Date: Wed Feb 9 12:35:28 2011 Subject: [Histonet] CPT code 88363 Message-ID: <8D6EDB72CECABB4A988C5BB087B61E4F01032BE3@mghemail1.mgh.net> This can only be used for slide review prior to ordering molecular testing. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 662-4648 If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. From MadaryJ <@t> MedImmune.com Wed Feb 9 12:36:10 2011 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Feb 9 12:36:16 2011 Subject: [Histonet] microm parts for microtomes, only through Thermo? Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A13033CB590@MD1EV002.medimmune.com> Hi All, I have a blade assembly that is giving grief. I would like to just adjust the set screws to fix it and will try. In checking for a new assembly I got a price of 2220 for just the blade holder. Are there any companies out there that manufacture after market products like this? Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From sgoebel <@t> mirnarx.com Wed Feb 9 12:47:13 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Feb 9 12:47:17 2011 Subject: [Histonet] microm parts for microtomes, only through Thermo? In-Reply-To: <29A3CB81288E6F4BA2C9B3C8015A9A13033CB590@MD1EV002.medimmune.com> References: <29A3CB81288E6F4BA2C9B3C8015A9A13033CB590@MD1EV002.medimmune.com> Message-ID: I just ordered one from VWR. It's for a thermo tome which I think will fit on microms? It was $1600. I would call a tech help and see what they would suggest. Another thought would be to call IMEB (specifically Denise deVines 18005438496). I have had lots of good luck with the company and Denise is great! They do a lot of refurbishing of equipment and maybe she could help you out or at least point you in the right direction. Ok Denise...I get a cookie with my next order right =) Note: I am in no way affiliated with IMEB or getting anything for this suggestion, I just think it's a great company and wanted to pass it along to everyone!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Wednesday, February 09, 2011 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microm parts for microtomes, only through Thermo? Hi All, I have a blade assembly that is giving grief. I would like to just adjust the set screws to fix it and will try. In checking for a new assembly I got a price of 2220 for just the blade holder. Are there any companies out there that manufacture after market products like this? Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Wed Feb 9 12:52:19 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Feb 9 12:52:25 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <4D52CE6C.2030908@pathology.washington.edu> References: <4A645070A2B746CA9B569BAA6DA6FDBC@lurie.northwestern.edu> <4D52CE6C.2030908@pathology.washington.edu> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139ED53834@NADCWPMSGCMS03.hca.corpad.net> In Tissue Tek block cabinets, front (smallest number) to back (larger numbers) with numbers facing right side up as you pull out the drawer. Does that make sense? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, February 09, 2011 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette labeling For those of you that answered with B, how are you filing the blocks? Just curious. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/9/2011 9:24 AM, Bernice Frederick wrote: > B > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab > Sent: Wednesday, February 09, 2011 11:15 AM > To: 'Lee Mayhew'; Histonet > Subject: RE: [Histonet] Cassette labeling > > "A" For filing reasons. When we file in the boxes on top of microtome they > are right side up and for final filing in the plastic drawers writing is > right side up. > > Dawn > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew > Sent: Tuesday, February 08, 2011 3:55 PM > To: Histonet > Subject: [Histonet] Cassette labeling > > Hi Histonetters, > > At my hospital, we are having a discussion about how to label cassettes. I > have worked at 2 hospitals, and they each do it a different way. Our > cassette labeller will print either way. > > Could you please indicate which way you do it at your site, A or B. > > A......When the cassette is open and sitting on the bench facing you > with the lid on the far side and the surface for writing on is closest to > you, the surgical number is upside down. > > B.....When the cassette is open and sitting on the bench facing you > with the lid on the far side and the surface for writing on is closest to > you, the surgical number is right side up. > > Thanks in advance. > > Lee Mayhew MLT > St. Josephs Hospital > Hamilton, ON Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the > designated recipient(s) named above. If you are not the intended recipient, > you are hereby notified that you have received this communication in error > and that any review, disclosure, dissemination, distribution or copying of > it or its contents is prohibited. If you have received this communication in > error, please notify the sender at the electronic mail address noted above > and destroy all copies of this communication and any attachments. Thank you > for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmashore <@t> vapop.ucsd.edu Wed Feb 9 12:59:46 2011 From: mmashore <@t> vapop.ucsd.edu (Michael Mashore) Date: Wed Feb 9 13:00:04 2011 Subject: [Histonet] core histolgy recharge rates Message-ID: Hello all, I am trying to get a feel for how other core histology facilities are charging. My main question is if or when technician's labor time is charged. Currently we charge a fee per sample for embedding, whether it is paraffin, plastic, or oct. Then we charge a fee per slide/grid for cutting, and fee per slide/grid for staining. Any time the technician is doing the work there is also a labor/tech time fee per hour. Any information you are willing to share is very much appreciated. Thanks, Michael From AHutton <@t> dh.org Wed Feb 9 13:00:04 2011 From: AHutton <@t> dh.org (Hutton, Allison) Date: Wed Feb 9 13:02:08 2011 Subject: [Histonet] cell block fixation Message-ID: <38A56C4F4630D348A50B3720409270870E0FE2D4@dhmail.dhorg.org> We recently switched vendors for our formalin and while we have not experienced any difference with our surgical specimens, our cell blocks from body fluids have been giving us a great deal of trouble. The button that we get never seems to harden, leaving it sort of gelatinous, even if left to sit in formalin for days. We are able to get sections off of these cell blocks, however, the slides are blank by the end of the staining process. This is only a recent development that seems to coincide with the time we switched formalin vendors and it only happens with body fluid specimens (FNA specimens don't seem to give us as much trouble). The composition of the formalin is almost identical between vendors. Can anyone help me explain why this might be happening? Thank you in advance, Allison From CIngles <@t> uwhealth.org Wed Feb 9 13:34:52 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Feb 9 13:37:24 2011 Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI References: <20110208032121.8D2865331B@qmail2.topechelon.com> <996734E2-2E05-4419-8E94-2A692983B88E@slonepartners.com> <8C023B4AB999614BA4791BAEB26E273839A1C7@UWHC-MAIL01.uwhis.hosp.wisc.edu> <79CAEF57-38AE-43BC-8440-99D6BF7D2771@slonepartners.com> Message-ID: Sorry gang: the position in Madison wasn't the one I was thinking of. Disregard warning. It is the position for the surgical path manager at UW Hospital in Madison I was talking about. Claire ________________________________ > > I think you are confusing this position with a different position in > Madison. The supervisor in this position will oversee Histology > including IHC and the gross room. There are no autopsies and no renal > lab. The ideal candidate will have experience with LEAN or workflow > redesign. I look forward to speaking with anyone who is qualified and > would be happy to give you more details. > > Regards, > > Therese > Slone Partners > > On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote: > >> Sorry Therese, but - >> Only consider this position if you like to be Really busy. As I > respect all on this list. I will issue a warning that this position is > much more involved than the 'ad' says. You would be overseeing the Histo > lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and > have daily contact with the pathologists and many others. Contact me for > details if you need to. Bob Merril (if anyone knows him) had this job, > but he has retired. >> Claire >> UW Hospital and Clinics >> Madison WI >> >> ________________________________ >> >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Therese > Cook >> Sent: Mon 2/7/2011 9:21 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Seeking a Surgical Pathology Supervisor in > Madison, WI >> >> >> >> >> Slone Partners=eeks a Surgical Pathology Supervisor for our hospital >> client, located=n Madison, Wisconsin. >> >> >> >> This key management position, will=versee the staff and the day >> to day operations of this busy department. =athology Assistants or >> HT and supervisory experience are required. A BS=s preferred. >> >> >> >> If you are energetic, have great communication=kills, and a >> positive attitude, this might be the position for you. >> >> >> >> Qualified candidates should send their resume to Therese=ook at >> [1]theresec@slonepartners.com. >> >> >> >> If you do not meet these qualifications, but wish to be considered > for other roles in the laboratory diagnostic industry, please forward >> your=esume to Tara Kochis at [2]tara@slonepartners.com. >> >> >> >> All inquiries are kept confidential. >> >> References >> >> 1. > 3D"mailto:theresec@slonepartners.com?subject=New%20Candidate%20Request%2 > 0%28Surgical%20Pathology%20Supervisor%20-%201455%29" >> 2. > 3D"mailto:tara@slonepartners.com?subject=New%20Candidate%20Request%20"__ > _____________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > SLONEPARTNERS > THERESE COOK - EXECUTIVE RECRUITER > > Corporate Headquarters > 1521 Alton Road #638 > Miami Beach, Florida 33139 > > TOLL FREE: 877.419.1439 > DIRECT: 330.863.1054 > CELL: 330.323.4525 > FAX: 330.232.9333 > > www.slonepartners.com > > > SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX: 330.232.9333 www.slonepartners.com From rjbuesa <@t> yahoo.com Wed Feb 9 14:26:55 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 9 14:27:00 2011 Subject: [Histonet] IHC validation In-Reply-To: <7C7A9E0F28DE4EC3A19B0A8C4E63F4CF@JoePC> Message-ID: <605752.32332.qm@web65713.mail.ac4.yahoo.com> Joe: This is what I would do; 1- run 1 control slide per antibody you have in your "arsenal" 2- compare the result with a control slide already in your files. 3- show both slides to the chief pathologist (after all is his/her opinion the one is going to be asked by CAP) 4- those antibodies whose positive controls reacted substantially different to those in your files are the ones you have to work with with respect to concentration or detection method. 5- never overdue it, and avoid excessive costs that usually are never appreciated. Rely always in your pathologist's opinion Ren? J. --- On Tue, 2/8/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] IHC validation To: "Histonet" Date: Tuesday, February 8, 2011, 5:43 PM Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we? start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. ? ? The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 9 14:32:26 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 9 14:32:29 2011 Subject: [Histonet] Nickel-DAB Signal Fading In-Reply-To: Message-ID: <989628.73492.qm@web65716.mail.ac4.yahoo.com> If when you finished the procedure the signal was strong, it is not a problem with the antibody, it would have reacted with your working concentration, or would not have worked. I am inclined to think that the nickel solution you use to add to the DAB may have deteriorated in that batch and have caused the fading. Try some of the block to cut new sections and use a?fresh nickel solution to find out what happens in 2 months time. Ren? J. ? ? --- On Tue, 2/8/11, Amanda Madden wrote: From: Amanda Madden Subject: [Histonet] Nickel-DAB Signal Fading To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 6:27 PM Hello Histonetters! I am once again completely baffled, and thought you might be able to help. I run immunocytochemistry on rodent brain tissue every week, always using the exact same procedures, solutions, buffers, chromagen, and mounting medium. The only variable is the primary and secondary antibodies. Our protocol has been time tested and extremely successful. I recently ran a large batch of tissue, and the staining looked great... except that now, about two months later, the signal has faded almost to the point of being gone. I am using Nickel intensified DAB, if that helps, and coverslipping (after an alcohol series finished with xylene) with Permount. Could it be a problem with the primary antibody (one which is new to us but has seemed fine in series dilutions)? Or is it a problem with the chromagen/mounting medium? I have been storing the slides in slides boxes so I don't think it is light. Going crazy, and thankful for any advice, Amanda Madden _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Feb 9 14:36:13 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Feb 9 14:36:19 2011 Subject: [Histonet] IHC validation In-Reply-To: <605752.32332.qm@web65713.mail.ac4.yahoo.com> References: <7C7A9E0F28DE4EC3A19B0A8C4E63F4CF@JoePC> <605752.32332.qm@web65713.mail.ac4.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A71C@CHEXCMS10.one.ads.che.org> You know, I can see all this validation if you harvest your own abs and/or use concentrates, etc. And also for the FDA approved abs, but I don't see it if we use RTU abs that the company has already validated and we are just confirming it works in our lab. Seems overkill to me. I like that Rene says it this way! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 09, 2011 15:27 To: Histonet; Joe Nocito Subject: Re: [Histonet] IHC validation Joe: This is what I would do; 1- run 1 control slide per antibody you have in your "arsenal" 2- compare the result with a control slide already in your files. 3- show both slides to the chief pathologist (after all is his/her opinion the one is going to be asked by CAP) 4- those antibodies whose positive controls reacted substantially different to those in your files are the ones you have to work with with respect to concentration or detection method. 5- never overdue it, and avoid excessive costs that usually are never appreciated. Rely always in your pathologist's opinion Ren? J. --- On Tue, 2/8/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] IHC validation To: "Histonet" Date: Tuesday, February 8, 2011, 5:43 PM Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we? start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. ? ? The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rjbuesa <@t> yahoo.com Wed Feb 9 14:36:22 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 9 14:36:26 2011 Subject: [Histonet] PAS with Diastase Digestion In-Reply-To: Message-ID: <530354.118.qm@web65701.mail.ac4.yahoo.com> You CAN NOT use water to dissolve the diastase. You have to use diastase buffer pH7 otherwise it will not work (as in your case). Ren? J. --- On Wed, 2/9/11, Erin Sarricks wrote: From: Erin Sarricks Subject: [Histonet] PAS with Diastase Digestion To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 9, 2011, 7:38 AM Hi Histonet- I recently ran a PAS/D stain and had some issues with it.? Both "with" and "without" slides came out looking the same so I'm guessing my digestion step didn't work!? I used a Malt Diastase solution (0.5g to 500mL water) for my digestion.? This is the procedure I used: 1.? ? Deparaffinize and hydrate to water. 2.? ? Place the sections labeled ?with? in diastase solution preheated to 37?C for 1 hour.? Hold the sections labeled ?without? in distilled water. 3.? ? Wash in running water for 5 minutes 4.? ? Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5.? ? Wash in 3 changes of distilled water 6.? ? Place in Schiff reagent for 15 minutes 7.? ? Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8.? ? Counterstain in Mayer?s Hematoxylin for 3 minutes. 9.? ? Wash in tap water for 10 minutes 10.???Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long?? Any tips would be much appreciated!? Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort!? Haha!? Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarricks@us.army.mil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. http://autos.yahoo.com/new_cars.html From mward <@t> wfubmc.edu Wed Feb 9 14:46:04 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed Feb 9 14:46:20 2011 Subject: [Histonet] IHC validation In-Reply-To: <605752.32332.qm@web65713.mail.ac4.yahoo.com> References: <7C7A9E0F28DE4EC3A19B0A8C4E63F4CF@JoePC> <605752.32332.qm@web65713.mail.ac4.yahoo.com> Message-ID: What a sensible process! I agree with your approach. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 09, 2011 3:27 PM To: Histonet; Joe Nocito Subject: Re: [Histonet] IHC validation Joe: This is what I would do; 1- run 1 control slide per antibody you have in your "arsenal" 2- compare the result with a control slide already in your files. 3- show both slides to the chief pathologist (after all is his/her opinion the one is going to be asked by CAP) 4- those antibodies whose positive controls reacted substantially different to those in your files are the ones you have to work with with respect to concentration or detection method. 5- never overdue it, and avoid excessive costs that usually are never appreciated. Rely always in your pathologist's opinion Ren? J. --- On Tue, 2/8/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] IHC validation To: "Histonet" Date: Tuesday, February 8, 2011, 5:43 PM Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we? start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. ? ? The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Feb 9 14:43:15 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Feb 9 14:46:27 2011 Subject: [Histonet] CPT code 88363 In-Reply-To: <4D5292A4.7400.0077.1@harthosp.org> References: <4D5292A4.7400.0077.1@harthosp.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DDE30DD0@LRGHEXVS1.practice.lrgh.org> It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing "old" pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From gagnone <@t> KGH.KARI.NET Wed Feb 9 15:07:06 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Feb 9 15:07:16 2011 Subject: [Histonet] cassette/slide labeling and Vantage system Message-ID: Hi Carol, We too have implemented the Ventana Vantage system. While it doesn't necessarily directly increase throughput, the greatest benefit is time you don't realize is being saved. All that running around trying to correct incorrectly-labeled slides, block mixups and cases that escape through all your current error-traps and make it to the pathologist, only to be brought back to be remedied. That huge waste of time is gone. Of course, it's still possible to place the wrong slide label on the wrong slide at microtomy. There are process steps to minimize this, but it is possible. For slide labelling at microtomy, it is a time saving, plus reduces writer's cramp. There is no way to label half your blocks on a case with the wrong case number or block numbers. It is also a huge benefit when cutting special stains or IHC. The stains/markers ordered by the pathologist appear as labels to print. If a different block than the one requested is scanned, those labels just do not appear, so that's an indication an error may be about to happen if you proceed. It can also be used as a means of monitoring individual productivity. As each user signs in to a workstation and scans a block at microtomy, embedding etc, this is tracked by user and time of day. I would recommend it. Hope this helps, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From mikoff <@t> pacbell.net Wed Feb 9 15:28:40 2011 From: mikoff <@t> pacbell.net (Keith) Date: Wed Feb 9 15:28:45 2011 Subject: [Histonet] CPT code 88363- RE: Histonet Digest, Vol 87, Issue 18, Message-ID: <4D5292A4.7400.0077.1@harthosp.org> In-Reply-To: <201102092040.p19Kev8Q030213@flpd240.prodigy.net> Message-ID: <2FFE3030009049FB95F032E72D72AE78@kitchen> As defined at the AMA site, you have a valid use of the CPT code 88363 to claim for a review of "old," previously diagnosed pathology slides prior to ordering IHC testing. If reviewing for previously DX'd slides is not enough, IHC is tagging the molecules in the cells at the binding site. AMA- Examination and selection of retrieved archival (ie, previously diagnosed) tissue(s) for molecular analysis (eg, KRAS mutational analysis) Source- https://catalog.ama-assn.org/Catalog/cpt/cpt_search_result.jsp?_requestid=51 3040 5. CPT code 88363 (Richard Cartun) 10. CPT code 88363 (Lake,Debbie) Keith M. Mikoff, HTL(ASCP)(ret) ------------------------------ Message: 5 Date: Wed, 09 Feb 2011 13:12:05 -0500 From: "Richard Cartun" Subject: [Histonet] CPT code 88363 To: "Histonet" Message-ID: <4D5292A4.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII Is anyone using the above CPT code for reviewing "old" pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 10 Date: Wed, 9 Feb 2011 13:34:27 -0500 From: "Lake,Debbie" Subject: [Histonet] CPT code 88363 To: Message-ID: <8D6EDB72CECABB4A988C5BB087B61E4F01032BE3@mghemail1.mgh.net> Content-Type: text/plain; charset="us-ascii" This can only be used for slide review prior to ordering molecular testing. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 662-4648 From jaustin1967 <@t> gmail.com Wed Feb 9 15:30:18 2011 From: jaustin1967 <@t> gmail.com (Michael Bradley) Date: Wed Feb 9 15:30:22 2011 Subject: [Histonet] QIHC Message-ID: Hi all I am preparing to take the QIHC exam. Does anyone have any suggestions on what I should study? What areas does the exam concentrate on and what research material I can use. Thanks From bakevictoria <@t> gmail.com Wed Feb 9 15:37:29 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Feb 9 15:37:35 2011 Subject: [Histonet] core histolgy recharge rates In-Reply-To: References: Message-ID: Hi- A flat fee per slide was charged which included all *technical* preparation for the final slide. Also prior to the start of a project a cost estimate was compiled and submitted for review/approval. As portions of projects were completed we submit via the grant or project number the work completed and $$'s were deducted from that cost center. We did do a number of different types of tests/assays and if we were approached for a new type of assay then we would have to put together a cost analysis based on the needs of the project. Incorporated into that cost would be the technical/labor component also including whatever overhead percentage was required by the facility. This worked out well as I had everything done 'up front' and knew what was budgeted. We did have to submit a quarterly report of all work/$$'s to the government and by doing a flat fee it made life much easier. Vikki On Wed, Feb 9, 2011 at 1:59 PM, Michael Mashore wrote: > Hello all, > > I am trying to get a feel for how other core histology facilities are > charging. My main question is if or when technician's labor time is > charged. > Currently we charge a fee per sample for embedding, whether it is paraffin, > plastic, or oct. Then we charge a fee per slide/grid for cutting, and fee > per slide/grid for staining. Any time the technician is doing the work > there > is also a labor/tech time fee per hour. > > Any information you are willing to share is very much appreciated. > > Thanks, > > Michael > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From abright <@t> brightinstruments.com Wed Feb 9 16:08:08 2011 From: abright <@t> brightinstruments.com (abright@brightinstruments.com) Date: Wed Feb 9 16:08:21 2011 Subject: [Histonet] Tape transfer In-Reply-To: <20110209093044.19282fuvp27e2ugk@webmail.ugent.be> References: <20110209093044.19282fuvp27e2ugk@webmail.ugent.be> Message-ID: <1186236837-1297289292-cardhu_decombobulator_blackberry.rim.net-1826178575-@b26.c2.bise7.blackberry> Dear Nele, I hear of these problems very often. It would be worth your time looking for any information on fresh frozen bone sections by Dr. Dodds, there should be a number of papers he has published on good quality bone sectioning without the use of the slow, expensive and troublesome tape method. Alan Bright www.brightinstruments.com Sent from my BlackBerry? wireless device -----Original Message----- From: "Nele Degryse" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 09 Feb 2011 09:30:44 To: Subject: [Histonet] Tape transfer Hello, I'm trying to cut undecalcified bone (mouse knees) by using the tape transfer method. The problem is that the area that I'm interested in (the joint with patella) is not transferred from the tape on my slide. I've used slides with different coating (also those specially made for bone) and tried different thickness. None of that seems to make a difference. Another problem is that my hand roller started to stick, so I cleaned it with 100% ethanol (as recommended) and now it sticks even more... Can anybody give me some advice? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01E5IwhGp) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01E5IwhGp&m=9503c828d7b7&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01E5IwhGp&m=9503c828d7b7&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01E5IwhGp&m=9503c828d7b7&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From PMonfils <@t> Lifespan.org Wed Feb 9 17:11:43 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Feb 9 17:12:36 2011 Subject: [Histonet] PAS with Diastase Digestion In-Reply-To: References: Message-ID: <4EBFF65383B74D49995298C4976D1D5E07C09958@LSRIEXCH1.lsmaster.lifespan.org> Did you run a glycogen-positive control slide? I'm probably stating the obvious, but the "with" and "without" slides should look the same if there was no glycogen in the tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both "with" and "without" slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1. Deparaffinize and hydrate to water. 2. Place the sections labeled ?with? in diastase solution preheated to 37?C for 1 hour. Hold the sections labeled ?without? in distilled water. 3. Wash in running water for 5 minutes 4. Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5. Wash in 3 changes of distilled water 6. Place in Schiff reagent for 15 minutes 7. Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8. Counterstain in Mayer?s Hematoxylin for 3 minutes. 9. Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarricks@us.army.mil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Feb 9 18:01:56 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Feb 9 18:02:16 2011 Subject: [Histonet] Benchmark Problems In-Reply-To: References: Message-ID: <03138533E34F435185F40113B96E3DD2@JoePC> I would also see if Ventana can send you some test slides to test the heating. At my old lab, we had a problem with the heating plates. They repaired the entire platform. Just a thought. Joe ----- Original Message ----- From: "Mark Tarango" To: "Dessoye, Michael J" Cc: Sent: Wednesday, February 09, 2011 10:58 AM Subject: Re: [Histonet] Benchmark Problems > Hi Mike, > > Did you wash the bottles and rinse them and then pump the water through > the > instrument before adding reagents and purging all again? We had a problem > like this about 2 weeks ago. I think someone loaded the wrong bulk > reagent > onto the instrument. Cleaning it, purging with water and adding newly > made > bulk reagents fixed the problem for us. > > Mark > > On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J > wrote: > >> Hello, >> >> We started having a strange problem with our Ventana Benchmark XT. Out >> of >> the blue, our slides stopped staining. With most antibodies we get no >> reaction, some that were very strongly positive are now only very lightly >> staining. They do appear to counterstain OK. Following Ventana's >> recommendations we completely changed out all of our bulk fluids, purged >> the >> lines, changed antibodies and DAB kits. The last shot is some kind of >> instrument issue that's not triggering an error. Anyone ever see >> anything >> like this? >> >> Mike Dessoye >> _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ >> _ >> >> This email and any files transmitted with it are confidential and >> intended solely for the use of the individual or entity to whom >> they are addressed. >> If you have received this email in error please notify the >> originator of the message. This footer also confirms that this >> email message has been scanned for the presence of computer viruses. >> >> Any views expressed in this message are those of the individual >> sender, except where the sender specifies and with authority, >> states them to be the views of Wyoming Valley Health Care System. >> >> Scanning of this message and addition of this footer is performed >> by Websense Email Security software in conjunction with >> virus detection software. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Feb 9 16:46:31 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Feb 9 18:13:30 2011 Subject: [Histonet] RE: One more thing...I feel like Columbo In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A659@CHEXCMS10.one.ads.che.org> Message-ID: Sakura also has the heated forceps that plug directly into the embedding center. "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/09/2011 09:23 AM To "sgoebel@mirnarx.com" , "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] RE: One more thing...I feel like Columbo I think you can get from Thermo Scientific. Two sites found on line..j http://www.medite.de/ebp50.html?&L=1 https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=L10831&categoryId=81937&productId=12706060 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, February 09, 2011 11:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Feb 9 18:41:35 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Feb 9 18:41:55 2011 Subject: [Histonet] RE: Tape Transfer Message-ID: <000401cbc8bb$473709e0$d5a51da0$@callis@bresnan.net> You Wrote (two messages) Dear Nele, I hear of these problems very often. It would be worth your time looking for any information on fresh frozen bone sections by Dr. Dodds, there should be a number of papers he has published on good quality bone sectioning without the use of the slow, expensive and troublesome tape method. Alan Bright www.brightinstruments.com Sent from my BlackBerryR wireless device -----Original Message----- From: "Nele Degryse" <@t> UGent.be> Sender: histonet-bounces <@t> lists.utsouthwestern.edu Date: Wed, 09 Feb 2011 09:30:44 To: <@t> lists.utsouthwestern.edu> Subject: [Histonet] Tape transfer Hello, I'm trying to cut undecalcified bone (mouse knees) by using the tape transfer method. The problem is that the area that I'm interested in (the joint with patella) is not transferred from the tape on my slide. I've used slides with different coating (also those specially made for bone) and tried different thickness. None of that seems to make a difference. Another problem is that my hand roller started to stick, so I cleaned it with 100% ethanol (as recommended) and now it sticks even more... Can anybody give me some advice? **************************************************************************** *********************************** First, I totally disagree that using the "slow, expensive and troublesome" tape method is a problem. I took Dodd's workshop and then attempted cut bone frozen sections in our lab using his methods which proved to be a nightmare, taking hours to get one decent section IF we were lucky. We never "got lucky" in obtaining a an intact bone section that was free from folding and totally disrupted morphology. If any method is troublesome, it is the Dodd's method plus the expense of wasting time and nothing to show for it. The only thing I have used with any success was snap freezing bone the way he taught us, but that has gone by the wayside due to the toxic hexane fumes. His way was a total failure in our laboratory and we bought the Cryojane to get decent bone sections. Cryojane requires practice, a bit of patience, fine tuning the little details for successful use and it is not as slow as people think. There are many labs who use it successfully including ours. First of all, Nele did not provide any details on how the Cryojane was being used other than the different polymer coated slides. So questions follow: What was the cryostat chamber temperature? Is this the same temperature of the sample, knife and UV light source? If the cryostat is older model, have you actually checked the temperatures? Is the bone fixed in formalin before snap freezing? And if the bone was fixed, was it cryoprotected with at least 20 to 30% sucrose (this takes a long time for NBF fixed bone)? What thicknesses did you try? Thinner is often better than thicker. Is the d profile tungsten carbide knife perfectly sharp when cutting the frozen bone sections? This means a new edge for sectioning, and very frequent reconditioning of the knife (DDK or Dorn and Hart sharpening services). Hopefully you have two knives. Have you tried multiple UV flashes? Two to three flashes usually help keep bone on the slide. Do you remove the tape INSIDE the cold cryostat environment, and at an angle from one corner of slide across the section, slowly and gently. Have you tried orienting the bone differently? Sometimes this helps, particularly when removing the tape from the bone. Is your mouse leg left articulated so you section through the bent knee joint (into patella)? It sounds as though you have a temperature problem IF things are sticking. When washing the roller, are you putting a lot of alcohol on the roller, or simply wiping the roller with the alcohol. Is the roller at the exact cutting temperature of the sample before rolling the COLD tape onto the block face? More details will help since we can't see a real time session of what you are doing. Gayle M. Callis HTL/HT/MT(ASCP) From A.Mortimer <@t> latrobe.edu.au Wed Feb 9 20:21:54 2011 From: A.Mortimer <@t> latrobe.edu.au (Abbey Mortimer) Date: Wed Feb 9 20:22:02 2011 Subject: [Histonet] FFPE tissue - sucrose fixing necessary? Message-ID: Hello helfpul Histonetters! I am curious about your formalin-fixed paraffin embedded (FFPE) tissue protocols. If the tissue has been fixed with saline and paraformaldehyde during perfusion, then further fixed in paraformaldehyde for 24 hours, do you still utilise a sucrose wash before running it through the automatic tissue processor? Or do you not need this step? I have heard mixed responses about this and wonder if any of you have had experience with this! Thanks so much, Abbey From ccebjr <@t> embarqmail.com Wed Feb 9 20:22:16 2011 From: ccebjr <@t> embarqmail.com (Charles E. Brown, Jr.) Date: Wed Feb 9 20:22:21 2011 Subject: [Histonet] RE: Tape Transfer In-Reply-To: <000401cbc8bb$473709e0$d5a51da0$@callis@bresnan.net> References: <000401cbc8bb$473709e0$d5a51da0$@callis@bresnan.net> Message-ID: <075a01cbc8c9$563c5f90$02b51eb0$@embarqmail.com> Dear Colleagues Please remove my husband, Charles E Brown Jr. from you listings. My husband passed away on 1/27/11. Respectfully Mrs. Charles E Brown, Jr. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Wednesday, February 09, 2011 4:42 PM To: 'Histonet' Subject: [Histonet] RE: Tape Transfer You Wrote (two messages) Dear Nele, I hear of these problems very often. It would be worth your time looking for any information on fresh frozen bone sections by Dr. Dodds, there should be a number of papers he has published on good quality bone sectioning without the use of the slow, expensive and troublesome tape method. Alan Bright www.brightinstruments.com Sent from my BlackBerryR wireless device -----Original Message----- From: "Nele Degryse" <@t> UGent.be> Sender: histonet-bounces <@t> lists.utsouthwestern.edu Date: Wed, 09 Feb 2011 09:30:44 To: <@t> lists.utsouthwestern.edu> Subject: [Histonet] Tape transfer Hello, I'm trying to cut undecalcified bone (mouse knees) by using the tape transfer method. The problem is that the area that I'm interested in (the joint with patella) is not transferred from the tape on my slide. I've used slides with different coating (also those specially made for bone) and tried different thickness. None of that seems to make a difference. Another problem is that my hand roller started to stick, so I cleaned it with 100% ethanol (as recommended) and now it sticks even more... Can anybody give me some advice? **************************************************************************** *********************************** First, I totally disagree that using the "slow, expensive and troublesome" tape method is a problem. I took Dodd's workshop and then attempted cut bone frozen sections in our lab using his methods which proved to be a nightmare, taking hours to get one decent section IF we were lucky. We never "got lucky" in obtaining a an intact bone section that was free from folding and totally disrupted morphology. If any method is troublesome, it is the Dodd's method plus the expense of wasting time and nothing to show for it. The only thing I have used with any success was snap freezing bone the way he taught us, but that has gone by the wayside due to the toxic hexane fumes. His way was a total failure in our laboratory and we bought the Cryojane to get decent bone sections. Cryojane requires practice, a bit of patience, fine tuning the little details for successful use and it is not as slow as people think. There are many labs who use it successfully including ours. First of all, Nele did not provide any details on how the Cryojane was being used other than the different polymer coated slides. So questions follow: What was the cryostat chamber temperature? Is this the same temperature of the sample, knife and UV light source? If the cryostat is older model, have you actually checked the temperatures? Is the bone fixed in formalin before snap freezing? And if the bone was fixed, was it cryoprotected with at least 20 to 30% sucrose (this takes a long time for NBF fixed bone)? What thicknesses did you try? Thinner is often better than thicker. Is the d profile tungsten carbide knife perfectly sharp when cutting the frozen bone sections? This means a new edge for sectioning, and very frequent reconditioning of the knife (DDK or Dorn and Hart sharpening services). Hopefully you have two knives. Have you tried multiple UV flashes? Two to three flashes usually help keep bone on the slide. Do you remove the tape INSIDE the cold cryostat environment, and at an angle from one corner of slide across the section, slowly and gently. Have you tried orienting the bone differently? Sometimes this helps, particularly when removing the tape from the bone. Is your mouse leg left articulated so you section through the bent knee joint (into patella)? It sounds as though you have a temperature problem IF things are sticking. When washing the roller, are you putting a lot of alcohol on the roller, or simply wiping the roller with the alcohol. Is the roller at the exact cutting temperature of the sample before rolling the COLD tape onto the block face? More details will help since we can't see a real time session of what you are doing. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccebjr <@t> embarqmail.com Wed Feb 9 20:24:31 2011 From: ccebjr <@t> embarqmail.com (Charles E. Brown, Jr.) Date: Wed Feb 9 20:24:35 2011 Subject: [Histonet] RE: One more thing...I feel like Columbo In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E164081E09A659@CHEXCMS10.one.ads.che.org> Message-ID: <075b01cbc8c9$a6bda820$f438f860$@embarqmail.com> Dear Histonet Colleagues My husband, Charles E Brown Jr, passed away 1/27/11 Respectfully, Mrs. Charles E Brown Jr. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, February 09, 2011 2:47 PM To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: One more thing...I feel like Columbo Sakura also has the heated forceps that plug directly into the embedding center. "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/09/2011 09:23 AM To "sgoebel@mirnarx.com" , "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] RE: One more thing...I feel like Columbo I think you can get from Thermo Scientific. Two sites found on line..j http://www.medite.de/ebp50.html?&L=1 https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId= L10831&categoryId=81937&productId=12706060 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, February 09, 2011 11:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Thu Feb 10 02:13:06 2011 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Thu Feb 10 02:13:14 2011 Subject: [Histonet] PAS with Diastase Digestion In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2B3F04596A@FWDCWPMSGCMS09.hca.corpad.net> Not spit ,saliva and we leave it on for 15/20 minutes and then rinse well. I have had techs who did not like doing it so we bought the enzyme and after all the hassle she found it did not work as well...now we all think about chocolate and salivate. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both "with" and "without" slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1. Deparaffinize and hydrate to water. 2. Place the sections labeled ?with? in diastase solution preheated to 37?C for 1 hour. Hold the sections labeled ?without? in distilled water. 3. Wash in running water for 5 minutes 4. Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5. Wash in 3 changes of distilled water 6. Place in Schiff reagent for 15 minutes 7. Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8. Counterstain in Mayer?s Hematoxylin for 3 minutes. 9. Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarricks@us.army.mil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Thu Feb 10 02:21:59 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Thu Feb 10 02:24:15 2011 Subject: [Histonet] FFPE tissue - sucrose fixing necessary? In-Reply-To: References: Message-ID: This sounds like something you would do before freezing not processing to paraffin. Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbey Mortimer Sent: 10 February 2011 02:22 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE tissue - sucrose fixing necessary? Hello helfpul Histonetters! I am curious about your formalin-fixed paraffin embedded (FFPE) tissue protocols. If the tissue has been fixed with saline and paraformaldehyde during perfusion, then further fixed in paraformaldehyde for 24 hours, do you still utilise a sucrose wash before running it through the automatic tissue processor? Or do you not need this step? I have heard mixed responses about this and wonder if any of you have had experience with this! Thanks so much, Abbey _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From A.Mortimer <@t> latrobe.edu.au Thu Feb 10 03:41:37 2011 From: A.Mortimer <@t> latrobe.edu.au (Abbey Mortimer) Date: Thu Feb 10 03:42:02 2011 Subject: [Histonet] FFPE tissue - sucrose fixing necessary? In-Reply-To: References: , Message-ID: Thanks for your responses :) Abbey ________________________________________ From: Margaret Blount [mab70@medschl.cam.ac.uk] Sent: Thursday, 10 February 2011 7:21 PM To: Abbey Mortimer; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FFPE tissue - sucrose fixing necessary? This sounds like something you would do before freezing not processing to paraffin. Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbey Mortimer Sent: 10 February 2011 02:22 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE tissue - sucrose fixing necessary? Hello helfpul Histonetters! I am curious about your formalin-fixed paraffin embedded (FFPE) tissue protocols. If the tissue has been fixed with saline and paraformaldehyde during perfusion, then further fixed in paraformaldehyde for 24 hours, do you still utilise a sucrose wash before running it through the automatic tissue processor? Or do you not need this step? I have heard mixed responses about this and wonder if any of you have had experience with this! Thanks so much, Abbey _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> shorememorial.org Thu Feb 10 06:21:52 2011 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Thu Feb 10 06:22:39 2011 Subject: [Histonet] IHC validation In-Reply-To: <605752.32332.qm@web65713.mail.ac4.yahoo.com> Message-ID: Added to this.....................make sure you keep your work up sheets. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Rene J Buesa To Sent by: Histonet histonet-bounces@ lists.utsouthwest , Joe Nocito ern.edu cc Subject 02/09/2011 03:26 Re: [Histonet] IHC validation PM Joe: This is what I would do; 1- run 1 control slide per antibody you have in your "arsenal" 2- compare the result with a control slide already in your files. 3- show both slides to the chief pathologist (after all is his/her opinion the one is going to be asked by CAP) 4- those antibodies whose positive controls reacted substantially different to those in your files are the ones you have to work with with respect to concentration or detection method. 5- never overdue it, and avoid excessive costs that usually are never appreciated. Rely always in your pathologist's opinion Ren? J. --- On Tue, 2/8/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] IHC validation To: "Histonet" Date: Tuesday, February 8, 2011, 5:43 PM Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we? start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Feb 10 07:46:04 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Feb 10 08:19:26 2011 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: Read and digest the Dako book and you will sail through the exam. For a "specialist type" qualification it really is pitiful. Ronnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: Wednesday, February 09, 2011 4:30 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hi all I am preparing to take the QIHC exam. Does anyone have any suggestions on what I should study? What areas does the exam concentrate on and what research material I can use. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mpence <@t> grhs.net Thu Feb 10 08:19:37 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 10 08:19:42 2011 Subject: [Histonet] CPT code 88363 In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DDE30DD0@LRGHEXVS1.practice.lrgh.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B2A@is-e2k3.grhs.net> Is this a code that can be used even if you have your molecular done somewhere else? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, February 09, 2011 2:43 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] CPT code 88363 It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing "old" pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stephanie.d.rivera <@t> gsk.com Thu Feb 10 08:21:54 2011 From: stephanie.d.rivera <@t> gsk.com (Stephanie Rivera) Date: Thu Feb 10 08:22:02 2011 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: <14A77A757E86BC499A34E86B47649B64055C059421@019D-NAMSG-05.019D.MGD.MSFT.NET> Hi Michael, I used NSH 5th series assessment book, double check on the NSH website. It's the book with questions and answers for IHC, Enzyme histochemistry. I also used IHC tutorial by Thermo Shandon online, that was recommended on the histonet network. I'm not sure of the website. Maybe someone has that information. Both of these tools helped me when I passed in April 2009. Steph -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: Wednesday, February 09, 2011 4:30 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hi all I am preparing to take the QIHC exam. Does anyone have any suggestions on what I should study? What areas does the exam concentrate on and what research material I can use. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy_schmitt <@t> pa-ucl.com Thu Feb 10 09:09:47 2011 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Feb 10 09:09:54 2011 Subject: [Histonet] Cassette labeling In-Reply-To: <20110208232303.B412650C87@mail.pa-ucl.com> References: <20110208232303.B412650C87@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC06444D2667868@hestia.ad.pa-ucl.com> We use B. Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA Message: 9 Date: Tue, 8 Feb 2011 16:54:54 -0500 From: Lee Mayhew Subject: [Histonet] Cassette labeling To: Histonet Message-ID: <82D56D94-90D4-4137-88D1-4C0EDE21FCE1@cogeco.ca> Content-Type: text/plain; charset=us-ascii Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A......When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.....When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ------------------------------ NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Marcia_Gaiser <@t> ssmhc.com Thu Feb 10 09:59:06 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Thu Feb 10 09:59:13 2011 Subject: [Histonet] HT Position in Oklahoma City Message-ID: <728F817C02110E498D803A7C3B0C6248057D85BD56@S009-APEXM06.ds.ad.ssmhc.com> St. Anthony Hospital currently has an excellent opportunity for an experienced Histologic Technician. This position requires Certification as an HT or HLT ? or ? experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.com>, Ad # 10762, or contact Anna King for additional information at (405) 272-6105. Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From alyssa <@t> alliedsearchpartners.com Thu Feb 10 10:03:45 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Feb 10 10:03:52 2011 Subject: [Histonet] Supervisory/Lead Job in Fort Myers, FL Message-ID: *Position Title: *Histotech III (Lead)/Supervisory *Reports To: *Laboratory Director *Shift: *Monday-Friday, 8am-5pm * * *Location:* * * Pathology Laboratory for well established busy office in Fort Myers, FL founded in 2000. We are looking at local candidates for now, but may be willing to help with relocation of in state applicants. * * *Requirements:* * * - ASCP certification required** - Experience leading a team of 3+ laboratory staff memebers** - Experience and knowledge of OSHA, CLIA, AHCA regulations** - The ability to implement and maintain QA/QC policies and procedures** - Oversee safety and training** - Understanding of the different lab licensing and ranges of testing** * * *Summary:* * * - Responsible for 2 other employees within the laboratory** - Work with the Director of Compliance on compliancy of the lab** - Handles all laboratory compliance** - Occasionally help with day to day routine histology ** ** * * *Benefits:* Health Insurance, Dental Insurance, Vision Insurance. Group Life, Short Term Disability and Long Term Disability paid by employer. Voluntary Life for self, spouse, and child. Discount on employer products and services. PTO for vacation, personal time, sick etc. Paid Holidays (7): New Years Day, Memorial Day, Fourth of July, Labor Day, Thanksgiving Day, Day after Thanksgiving Day, and Christmas Day. Bereavement Leave.* *Bi-annual bonuses, performance reviews, uniform and name badge. Direct Depost & 401K with employer contribution. *To apply:* * * Please send resume and salary expectations to Alyssa@alliedsearchpartners.com . At that time we will contact you to conduct a phone screen. Thank you! -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From Rcartun <@t> harthosp.org Thu Feb 10 10:23:08 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 10 10:23:15 2011 Subject: [Histonet] CPT code 88363 In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974B2A@is-e2k3.grhs.net> References: <38667E7FB77ECD4E91BFAEB8D986386323DDE30DD0@LRGHEXVS1.practice.lrgh.org> <661949901A768E4F9CC16D8AF8F2838C03974B2A@is-e2k3.grhs.net> Message-ID: <4D53CA9C.7400.0077.1@harthosp.org> I think so. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Mike Pence" 2/10/2011 9:19 AM >>> Is this a code that can be used even if you have your molecular done somewhere else? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, February 09, 2011 2:43 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] CPT code 88363 It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing "old" pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Thu Feb 10 10:58:10 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Feb 10 10:58:14 2011 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: Its true, that test is too easy if you ask me. Dako handbook is all you'll need. Mark On Thu, Feb 10, 2011 at 5:46 AM, Houston, Ronald < Ronald.Houston@nationwidechildrens.org> wrote: > Read and digest the Dako book and you will sail through the exam. For a > "specialist type" qualification it really is pitiful. > > Ronnie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Bradley > Sent: Wednesday, February 09, 2011 4:30 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] QIHC > > Hi all > > I am preparing to take the QIHC exam. Does anyone have any suggestions on > what I should study? What areas does the exam concentrate on and what > research material I can use. > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Annette.Fletcher <@t> providence.org Thu Feb 10 12:22:08 2011 From: Annette.Fletcher <@t> providence.org (Fletcher, Annette M) Date: Thu Feb 10 12:22:22 2011 Subject: [Histonet] Histotech 2 position available in Portland, OR with Providence Health and Services Message-ID: <664BDC4CEA139D49BB40D4B019AFBBF711CA2C63@WNP5088.or.providence.org> Dear Histology Professionals, (Please, no Recruiters) If you are interested in exploring a move to the Pacific NW, please email me regarding the following opportunity: Providence is calling a full-time Histotechnician II for an evening shift position at Providence Portland Medical Center in Portland, OR. Providence Health and Services in Oregon is seeking a passionate Histology professional to join our team in Pathology to accurately prepare material for study and diagnostic interpretation by pathologists. Histology personnel will perform pre-analytical, analytical and post-analytical duties including tissue processing, routine stains, special stains, immunohistochemical stains, instrument maintenance and problem resolution. Histology personnel work with acute-care and outpatient caregivers, vendors, consultants and other Laboratory Services staff in performing their duties. Our Pathology department serves 7 hospitals and multiple outpatient clinics in a challenging, state of the art department. We offer competitive compensation and a distinguished benefits plan. The requirements for this position include: * For HT, HTL with Bachelors degree: 2-5 years of industry related experience (**). For HT from accredited training program or OJT: 3-6 years of industry related (**) experience. (**) Industry related at Level 2 requires that the experience be from an equivalent high volume or acute care laboratory performing high complexity testing. Answer the call to fulfill the Mission. Providence Health & Services is a not-for-profit organization extending across a five-state area - from Alaska through Washington, Montana, Oregon, and into Southern California. Providence employs more than 45,000 employees, and operates 27 acute care hospitals, and more than 35 non-acute health care facilities, as well as physician clinics, health plans, and numerous other health and education services. When you're ready to pursue an outstanding career opportunity that offers more than a competitive salary and an excellent variety of benefits, consider Providence Health & Services-Oregon. Please send me your resume and/or any questions you have about this opportunity. Kind Regards, Annette M Fletcher Senior Recruiter Annette.fletcher@providence.org Providence Health & Services, Talent Acquisition Respect | Compassion | Justice | Excellence | Stewardship ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From michelecarr10 <@t> yahoo.com Thu Feb 10 12:29:11 2011 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Thu Feb 10 12:29:16 2011 Subject: [Histonet] p16 antibody Message-ID: <541084.35183.qm@web120701.mail.ne1.yahoo.com> Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services From bakevictoria <@t> gmail.com Thu Feb 10 12:31:56 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Feb 10 12:32:00 2011 Subject: [Histonet] p16 antibody In-Reply-To: <541084.35183.qm@web120701.mail.ne1.yahoo.com> References: <541084.35183.qm@web120701.mail.ne1.yahoo.com> Message-ID: Yes. They hold the patent. On Feb 10, 2011 1:30 PM, "Michele Carr" wrote: > Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? > Thanks in advance. > Michele Carr HTL ASCP > Medical Laboratory Services > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Thu Feb 10 12:33:05 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Feb 10 12:33:33 2011 Subject: [Histonet] p16 antibody In-Reply-To: <541084.35183.qm@web120701.mail.ne1.yahoo.com> References: <541084.35183.qm@web120701.mail.ne1.yahoo.com> Message-ID: <0B8979A204680A42B93A52B486088CD912940DFE01@CUAEXH1.GCU-MD.local> MTM has the patent rights so they are the only one. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Carr [michelecarr10@yahoo.com] Sent: Thursday, February 10, 2011 1:29 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From scorpionrider <@t> cox.net Thu Feb 10 12:36:36 2011 From: scorpionrider <@t> cox.net (Mark Turner) Date: Thu Feb 10 12:36:40 2011 Subject: [Histonet] p16 antibody In-Reply-To: <541084.35183.qm@web120701.mail.ne1.yahoo.com> Message-ID: <20110210133636.IBNDY.587426.imail@fed1rmwml4201> They hold the patent and no one else produces it. We order the kit and use the antibody on the Ventana XT stainers, placing the antibody in a prep kit. Works great. Mark Turner, PhD, HT(ASCP)QIHC Mayo Clinic in Arizona ---- Michele Carr wrote: > Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? > Thanks in advance. > Michele Carr HTL ASCP > Medical Laboratory Services > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ahacking <@t> yahoo.com Thu Feb 10 12:52:43 2011 From: ahacking <@t> yahoo.com (Adam Hacking) Date: Thu Feb 10 12:52:47 2011 Subject: [Histonet] tungsten carbide knives Message-ID: <298589.51870.qm@web30803.mail.mud.yahoo.com> Hi ? If?anyone has any used tungsten carbide knives that they no longer need please let me know.? These should be in good shape and suitable for sharpening. I'm happy to pay a resonable price for them. ? Thanks ? Adam --- On Thu, 2/10/11, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 20 To: histonet@lists.utsouthwestern.edu Date: Thursday, February 10, 2011, 1:51 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. RE: QIHC (Houston, Ronald) ???2. RE: CPT code 88363 (Mike Pence) ???3. RE: QIHC (Stephanie Rivera) ???4. Cassette labeling (Nancy Schmitt) ???5. HT Position in Oklahoma City (Gaiser, Marcia) ???6. Supervisory/Lead Job in Fort Myers, FL (Alyssa Peterson) ???7. RE: CPT code 88363 (Richard Cartun) ???8. Re: QIHC (Mark Tarango) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Thu Feb 10 12:53:02 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Feb 10 12:53:08 2011 Subject: [Histonet] Position Open in NJ/NY area Message-ID: Allied Search Partners is currently accepting resumes for a histotechnician/histotechnologist. Ideally we are looking for a candidate with at least 3 years experience. Location: Clifton, NJ area *Essential Functions and Duties* Must be able to work independently under minimal supervision, maintain laboratory supplies, equipment, and QC/QA records Must be knowledgeable with basic computer skills and pathology software, and participate in improvement of laboratory procedures. The person should be reliable with great inter-personal communication skills, and willing to coordinate with other departmental staff. Need someone with great cutting, grossing skills. *Shift:* Day Shift Hours *Requirements* Bachelor?s degree preferred but not required HT (ASCP) preferred The Bachelor degree may be waived if the candidate has extensive experience (>5 years) At least 3 years of histotechnology experience including routine histology, immunohistochemistry staining (automated), special stain (manual, for bone marrow and others). To apply for this position please submit resume to alyssa@alliedsearchpartners.com for initial prescreening. No resume will be submitted to client until we speak to you for a phone interview. All resumes kept confidential. -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From histonet.nospam <@t> vneubert.com Thu Feb 10 13:14:45 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Thu Feb 10 13:14:53 2011 Subject: [Histonet] Need help with interpretation: Gomori Trichrome / MELAS-Syndrom Message-ID: <4D543925.10708@vneubert.com> Hi all, I'm reading about OXPHOS diseases (defects in mitochondrial protein subunits of respiratory chain). RFF in MERRF stands for "red ragged fibers", see: http://en.wikipedia.org/wiki/File:Ragged_red_fibers_in_MELAS.jpg I don't get what is stained there exactly. The picture shows a muscle biopsy stained with Gomori's trichrome stain.. What is stained in these red fibers? I'm sure one of you has some experience with that. Thanks, V. Neubert From bseaton <@t> seattlecca.org Thu Feb 10 13:36:54 2011 From: bseaton <@t> seattlecca.org (Seaton, Brandon W) Date: Thu Feb 10 13:37:14 2011 Subject: [Histonet] bar-coded chemical inventory Message-ID: <8428_1297366615_4D543E57_8428_708_1_0F3F13441AD1CF41890BD0295EB7178203DA4EC2@EVS01.seattlecca.org> Anyone out there using barcode systems to inventory chemicals and organize MSDS, expiration dates, lot numbers, NFPA, etc? If so, what system and how well does it work? In particular I'm curious about the CISpro system. Many thanks! Brandon This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 624-1159, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From dreynold <@t> mdanderson.org Thu Feb 10 13:38:43 2011 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Thu Feb 10 13:38:46 2011 Subject: [Histonet] Help with OCT problem Message-ID: <785BBF0C5F49CE41BA74460A43A08F022B81E4E13F@DCPWVMBXC0VS3.mdanderson.edu> Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing helps. When I finally manage to get a decent section and pick it up on the slide, the OCT around the tissue want even pick up smooth it wrinkles really bad. I tried just a block of OCT that I froze on a chuck and it cut great. I am clueless as to what is going on. Samples were frozen by someone else and brought to me to be cut. Donna Reynolds Core IHC Lab M.D. Anderson Cancer Center, Houston TX Research lab 713-192-8106. Donna Reynolds Core IHC Lab Dept. Cancer Biology, SRB 1.660 713-792-8106 From liz <@t> premierlab.com Thu Feb 10 13:46:26 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Feb 10 13:46:31 2011 Subject: [Histonet] bar-coded chemical inventory In-Reply-To: <8428_1297366615_4D543E57_8428_708_1_0F3F13441AD1CF41890BD0295EB7178203DA4EC2@EVS01.seattlecca.org> Message-ID: I would be interested in that info too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Seaton, Brandon W Sent: Thursday, February 10, 2011 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bar-coded chemical inventory Anyone out there using barcode systems to inventory chemicals and organize MSDS, expiration dates, lot numbers, NFPA, etc? If so, what system and how well does it work? In particular I'm curious about the CISpro system. Many thanks! Brandon This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 624-1159, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Feb 10 13:49:08 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Feb 10 13:49:33 2011 Subject: [Histonet] Help with OCT problem In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F022B81E4E13F@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: Donna I have seen soft or rather sticky OCT samples in the past. If you freeze in isopentane and don't let the isopentane evaporate off the samples prior to wrapping the sample in foil, that excess isopentane changes the OCT, it makes it sticky. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M Sent: Thursday, February 10, 2011 12:39 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Help with OCT problem Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing helps. When I finally manage to get a decent section and pick it up on the slide, the OCT around the tissue want even pick up smooth it wrinkles really bad. I tried just a block of OCT that I froze on a chuck and it cut great. I am clueless as to what is going on. Samples were frozen by someone else and brought to me to be cut. Donna Reynolds Core IHC Lab M.D. Anderson Cancer Center, Houston TX Research lab 713-192-8106. Donna Reynolds Core IHC Lab Dept. Cancer Biology, SRB 1.660 713-792-8106 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Thu Feb 10 14:10:21 2011 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Feb 10 14:10:30 2011 Subject: [Histonet] Artisan Message-ID: <4D53F1CD.5D38.00EF.0@swmail.sw.org> Has anyone had background issues with the Gram stain on the Dako Artisan Link? If so - how was it resolved? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From dhewitt <@t> hvhs.org Thu Feb 10 14:25:06 2011 From: dhewitt <@t> hvhs.org (DANIEL HEWITT) Date: Thu Feb 10 14:25:10 2011 Subject: [Histonet] CPT code 88363 In-Reply-To: <4D53CA9C.7400.0077.1@harthosp.org> References: <38667E7FB77ECD4E91BFAEB8D986386323DDE30DD0@LRGHEXVS1.practice.lrgh.org><661949901A768E4F9CC16D8AF8F2838C03974B2A@is-e2k3.grhs.net> <4D53CA9C.7400.0077.1@harthosp.org> Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B9B72CB@MX-HVB-02.hvhs.org> How about pulling blocks and cutting blank slides for an attorney who does not represent our hospital? We don't have to do this very much but it does come up. Thanks Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 10, 2011 11:23 AM To: Mike Pence; Histonet; Thomas Podawiltz Subject: RE: [Histonet] CPT code 88363 I think so. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Mike Pence" 2/10/2011 9:19 AM >>> Is this a code that can be used even if you have your molecular done somewhere else? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, February 09, 2011 2:43 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] CPT code 88363 It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing "old" pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Feb 10 14:35:28 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 10 14:35:36 2011 Subject: [Histonet] CPT code 88363 In-Reply-To: <7DDB5AB36CBC574D8D680806E7BBE58B9B72CB@MX-HVB-02.hvhs.org> References: <38667E7FB77ECD4E91BFAEB8D986386323DDE30DD0@LRGHEXVS1.practice.lrgh.org><661949901A768E4F9CC16D8AF8F2838C03974B2A@is-e2k3.grhs.net> <4D53CA9C.7400.0077.1@harthosp.org> <7DDB5AB36CBC574D8D680806E7BBE58B9B72CB@MX-HVB-02.hvhs.org> Message-ID: <4D5405C0.7400.0077.1@harthosp.org> No; you can not bill the patient for this. You should charge the attorney for the work you do. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "DANIEL HEWITT" 2/10/2011 3:25 PM >>> How about pulling blocks and cutting blank slides for an attorney who does not represent our hospital? We don't have to do this very much but it does come up. Thanks Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 10, 2011 11:23 AM To: Mike Pence; Histonet; Thomas Podawiltz Subject: RE: [Histonet] CPT code 88363 I think so. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Mike Pence" 2/10/2011 9:19 AM >>> Is this a code that can be used even if you have your molecular done somewhere else? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, February 09, 2011 2:43 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] CPT code 88363 It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing "old" pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Thu Feb 10 14:47:56 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Feb 10 14:48:16 2011 Subject: [Histonet] Her2 requests on decal specimens Message-ID: I am hoping to get some guidance on this. Occasionally we get requests to perform HER2 on specimens that have been decaled. The revised CAP question, ANP.22998 specifically states in the notes that decalcification with strong acids should not be used. How are other institutions handling this? Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From foreightl <@t> gmail.com Thu Feb 10 14:51:37 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Thu Feb 10 14:51:42 2011 Subject: [Histonet] Help with OCT problem In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F022B81E4E13F@DCPWVMBXC0VS3.mdanderson.edu> References: <785BBF0C5F49CE41BA74460A43A08F022B81E4E13F@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: Donna, I would also check to make sure that the tissue wasn't held in an something with a lot of alcohol, like RNA later. I found out first hand that it doesn't work well. Good luck On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M wrote: > Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing helps. When I finally manage to get a decent section and pick it up on the slide, the OCT around the tissue want even pick up smooth it wrinkles really bad. > I tried just a block of OCT that I froze on a chuck and it cut great. ?I am clueless as to what is going on. > Samples were frozen by someone else and brought to me to be cut. > Donna Reynolds Core IHC Lab > M.D. Anderson Cancer Center, Houston TX > Research lab > 713-192-8106. > > > Donna Reynolds Core IHC Lab > Dept. Cancer Biology, SRB 1.660 > 713-792-8106 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From leiker <@t> buffalo.edu Thu Feb 10 15:34:45 2011 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Feb 10 15:34:52 2011 Subject: [Histonet] Help with OCT problem In-Reply-To: References: <785BBF0C5F49CE41BA74460A43A08F022B81E4E13F@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: I agree with previous responders to this query, as I've gotten alcohol on my blocks and since alcohol doesn't freeze real well it makes a sticky mess with the OCT. The affected OCT can be scraped off with a razor blade. Hopefully the tissue itself did not come in contact with alcohol prior to embedding. Regards, Merced --On Thursday, February 10, 2011 12:51 PM -0800 Patrick Laurie wrote: > Donna, > I would also check to make sure that the tissue wasn't held in an > something with a lot of alcohol, like RNA later. I found out first > hand that it doesn't work well. Good luck > > On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M > wrote: >> Has anyone ever experienced OCT blocks that cut like they are soft. I >> have turned the cyrostat down 10 degrees lower than usual and they are >> still soft. Really weird part is that I have 20 blocks in this group and >> some are soft and others are just fine. Tried changing angle, new blade >> nothing helps. When I finally manage to get a decent section and pick it >> up on the slide, the OCT around the tissue want even pick up smooth it >> wrinkles really bad. I tried just a block of OCT that I froze on a chuck >> and it cut great. ?I am clueless as to what is going on. Samples were >> frozen by someone else and brought to me to be cut. Donna Reynolds Core >> IHC Lab >> M.D. Anderson Cancer Center, Houston TX >> Research lab >> 713-192-8106. >> >> >> Donna Reynolds Core IHC Lab >> Dept. Cancer Biology, SRB 1.660 >> 713-792-8106 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology & Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > plaurie@cellnetix.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Wanda.Smith <@t> HCAhealthcare.com Thu Feb 10 15:56:07 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Feb 10 15:56:16 2011 Subject: [Histonet] CPT code 88363 In-Reply-To: <7DDB5AB36CBC574D8D680806E7BBE58B9B72CB@MX-HVB-02.hvhs.org> References: <38667E7FB77ECD4E91BFAEB8D986386323DDE30DD0@LRGHEXVS1.practice.lrgh.org><661949901A768E4F9CC16D8AF8F2838C03974B2A@is-e2k3.grhs.net> <4D53CA9C.7400.0077.1@harthosp.org> <7DDB5AB36CBC574D8D680806E7BBE58B9B72CB@MX-HVB-02.hvhs.org> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EDAF466@NADCWPMSGCMS03.hca.corpad.net> You charge the attorney's office "out the waazoo"!!! WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DANIEL HEWITT Sent: Thursday, February 10, 2011 3:25 PM To: Richard Cartun; Mike Pence; Histonet; Thomas Podawiltz Subject: RE: [Histonet] CPT code 88363 How about pulling blocks and cutting blank slides for an attorney who does not represent our hospital? We don't have to do this very much but it does come up. Thanks Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 10, 2011 11:23 AM To: Mike Pence; Histonet; Thomas Podawiltz Subject: RE: [Histonet] CPT code 88363 I think so. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Mike Pence" 2/10/2011 9:19 AM >>> Is this a code that can be used even if you have your molecular done somewhere else? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, February 09, 2011 2:43 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] CPT code 88363 It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing "old" pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Thu Feb 10 16:47:48 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Feb 10 16:49:53 2011 Subject: [Histonet] Help with OCT problem References: Message-ID: Same with ETOH. We wipe our surfaces down with it to clean them so no cross contamination as we freeze our skin here on the metal side areas of our cryostats. If the alcohol hasn't been wiped dry or evaporated we have that problem too. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Liz Chlipala Sent: Thu 2/10/2011 1:49 PM To: Reynolds,Donna M; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with OCT problem Donna I have seen soft or rather sticky OCT samples in the past. If you freeze in isopentane and don't let the isopentane evaporate off the samples prior to wrapping the sample in foil, that excess isopentane changes the OCT, it makes it sticky. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com From akbitting <@t> geisinger.edu Fri Feb 11 07:23:20 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Feb 11 07:23:34 2011 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: <4D54F1F8.2B7F.00C9.1@geisinger.edu> I thought the NSH Self Assessment CD was helpful too. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Mark Tarango 2/10/2011 11:58 AM >>> Its true, that test is too easy if you ask me. Dako handbook is all you'll need. Mark On Thu, Feb 10, 2011 at 5:46 AM, Houston, Ronald < Ronald.Houston@nationwidechildrens.org> wrote: > Read and digest the Dako book and you will sail through the exam. For a > "specialist type" qualification it really is pitiful. > > Ronnie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Bradley > Sent: Wednesday, February 09, 2011 4:30 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] QIHC > > Hi all > > I am preparing to take the QIHC exam. Does anyone have any suggestions on > what I should study? What areas does the exam concentrate on and what > research material I can use. > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From bennett777 <@t> gmail.com Fri Feb 11 08:58:04 2011 From: bennett777 <@t> gmail.com (Kevin Bennett) Date: Fri Feb 11 08:58:08 2011 Subject: [Histonet] succinate cytochrome c reductase Message-ID: Does anyone know of a protocol for the above stain or were I can find one. Need for neuromuscular histology... Thanks From member <@t> linkedin.com Fri Feb 11 08:58:47 2011 From: member <@t> linkedin.com (Natalia Zinchenko via LinkedIn) Date: Fri Feb 11 08:58:50 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <351304536.518047.1297436327150.JavaMail.app@ela4-bed40.prod> LinkedIn ------------Natalia Zinchenko requested to add you as a connection on LinkedIn: ------------------------------------------ Jackie, I'd like to add you to my professional network on LinkedIn. - Natalia Accept invitation from Natalia Zinchenko http://www.linkedin.com/e/yvpgd1-gk183942-1g/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1118717829_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPAOe3sNdPwNcj59bSlJlSdgsCtzbPoQejgMejcNcP8LrCBxbOYWrSlI/EML_comm_afe/ View invitation from Natalia Zinchenko http://www.linkedin.com/e/yvpgd1-gk183942-1g/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1118717829_3/3dvej8UdP4Te34NckALqnpPbOYWrSlI/svi/ ------------------------------------------ DID YOU KNOW LinkedIn can help you find the right service providers using recommendations from your trusted network? Using LinkedIn Services, you can take the risky guesswork out of selecting service providers by reading the recommendations of credible, trustworthy members of your network. http://www.linkedin.com/e/yvpgd1-gk183942-1g/svp/inv-25/ -- (c) 2011, LinkedIn Corporation From NMargaryan <@t> childrensmemorial.org Fri Feb 11 10:21:58 2011 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Feb 11 10:24:45 2011 Subject: [Histonet] Azure B as a counter-stain for melanin Message-ID: Dear Histology Professionals, I am using the Azure B as a counter-stain for melanin preparing solution by the protocol below. My questions are: 1. Is this solution should be fresh prepared every day? 2. How long I can store and use this solution? 3. Where this solution should be stored in the room or in the refrigerator? My protocol is: Counterstain Method The use of Azure B as a counter-stain will stain melanin a blue green color. Azure B working reagent: 4mL of a 25mg/mL aqueous solution of Azure B 3.4mL of 0.1M acetic acid 600uL of 0.1M sodium acetate 27mL of DI water 5mL acetone Incubate for up to 30 minutes, followed by a 20-second hematoxylin counter-stain. The use of either counterstain should give you good cytological detail, with blue-green melanin and brown DAB specific signal. Thanks in advance, Naira From Nacaela.Johnson <@t> USONCOLOGY.COM Fri Feb 11 11:03:47 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Fri Feb 11 11:03:50 2011 Subject: [Histonet] formal-acetone Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D524@txhous1eb012.uson.usoncology.int> Hello! We are beginning to optimize IHC staining for bone marrow smears. We are going to try a formal-acetone mixture for a fixative. Has anyone used this before? If so, can I get it pre-mixed or do I need to order them separate? Any suggestions on where to get it from? Does anyone have a procedure for fixation and IHC on bone marrow/ blood smears? Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From Ronald.Houston <@t> nationwidechildrens.org Fri Feb 11 11:25:32 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Feb 11 11:25:41 2011 Subject: [Histonet] RE: Azure B as a counter-stain for melanin In-Reply-To: References: Message-ID: Naira, we use a standard working Giemsa solution that is good for >1 month, stored at 4C. We automate the procedure on our Bonds: 3min Giemsa and 1 min Hematoxylin. Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, February 11, 2011 11:22 AM To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] Azure B as a counter-stain for melanin Importance: High Dear Histology Professionals, I am using the Azure B as a counter-stain for melanin preparing solution by the protocol below. My questions are: 1. Is this solution should be fresh prepared every day? 2. How long I can store and use this solution? 3. Where this solution should be stored in the room or in the refrigerator? My protocol is: Counterstain Method The use of Azure B as a counter-stain will stain melanin a blue green color. Azure B working reagent: 4mL of a 25mg/mL aqueous solution of Azure B 3.4mL of 0.1M acetic acid 600uL of 0.1M sodium acetate 27mL of DI water 5mL acetone Incubate for up to 30 minutes, followed by a 20-second hematoxylin counter-stain. The use of either counterstain should give you good cytological detail, with blue-green melanin and brown DAB specific signal. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From madelinegi <@t> yahoo.com Fri Feb 11 12:44:15 2011 From: madelinegi <@t> yahoo.com (Madeline Gi) Date: Fri Feb 11 12:44:18 2011 Subject: [Histonet] H&E stain uneven Message-ID: <249316.23274.qm@web59815.mail.ac4.yahoo.com> Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why.? Can someone tell me how does a routine H&E stain unevenly?? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated?. ? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com From jennifer.harvey <@t> Vanderbilt.Edu Fri Feb 11 12:49:04 2011 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Fri Feb 11 12:49:09 2011 Subject: [Histonet] mouse skeletal muscle frozen section prep In-Reply-To: <06B73B03-3C30-4027-8825-0924F57A0BBE@mimectl> Message-ID: Talc is used to reduce ice artifact in muscle. We are a clinical lab and receive muscles in various states. If the muscle is held in saline the chance for ice artifact increases greatly. We treat all muscles with talc just incase. You don't have to use a plastic mold or even totally coat the sample with OCT, freezing in liquid nitrogen is fine. Usually you do not fix the tissue before freezing but some research applications do. But one of the reasons to do frozens, is to avoid fixation. Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 On 2/8/11 9:51 AM, "Spinette, Sarah" wrote: > I am new to working with mouse skeletal muscle and have been reading about > various methods for preparing and freezing tissue for sectioning. > > I have heard of the benefits of coating the tissue in talc prior to freezing > but have not yet seen a single protocol that describes the use of talc and OCT > or other medium IN plastic molds, can you still use talc and then plunge the > tissue into cold OCT in the mold end then plunge the entire block into LiN2 > (we do not have isopentane)? > > Also, can anyone comment about when and how they choose to fix the tissue > first and if they use paraformaldehyde before freezing? > > Thank you! > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLashus <@t> pathgroup.com Fri Feb 11 12:52:04 2011 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Fri Feb 11 12:52:07 2011 Subject: [Histonet] H&E stain uneven In-Reply-To: <249316.23274.qm@web59815.mail.ac4.yahoo.com> References: <249316.23274.qm@web59815.mail.ac4.yahoo.com> Message-ID: <197CD0B02A81F94994A285C59C8AE05C0666F1E78B@pgnexchange.pathgroup.com> Have you checked the temperature of the heater used to dry you slides? We ran into this problem a couple of years ago and the temp of the oven was too high. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeline Gi Sent: Friday, February 11, 2011 1:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E stain uneven Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why. Can someone tell me how does a routine H&E stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated?. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From mfisher <@t> ecrmc.org Fri Feb 11 13:06:39 2011 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Fri Feb 11 13:13:33 2011 Subject: [Histonet] Decal Solution for Bone Marrows Message-ID: <3ACBB5D73A417547A01970CD3EB5509306EEC579@MAIL1.ecrmc.ci.el-centro.ca.us> I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From LSetlak <@t> childrensmemorial.org Fri Feb 11 13:21:31 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Fri Feb 11 13:21:37 2011 Subject: [Histonet] RE: Decal Solution for Bone Marrows In-Reply-To: <3ACBB5D73A417547A01970CD3EB5509306EEC579@MAIL1.ecrmc.ci.el-centro.ca.us> References: <3ACBB5D73A417547A01970CD3EB5509306EEC579@MAIL1.ecrmc.ci.el-centro.ca.us> Message-ID: <7111DB39D045004C9CF29E79C71B28BC100D15D9E5@CMHEXCC01MBX.childrensmemorial.org> We use "Immunocal" and we get ours from American Mastertech. We decal the cores for 3-3.5 hours. I can't speak to the Fe stains, but it does not interfere with any IHC's we do on them. Lisa V. Children's memorial Hospital Chg., IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Fisher Sent: Friday, February 11, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrows I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Fri Feb 11 13:23:50 2011 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Fri Feb 11 13:23:55 2011 Subject: [Histonet] Fw: Seeking equipment: leica SP 1600 Saw Microtome Message-ID: <5A982624491549A38E0CAC9ABD96AF57@vetmed.wisc.edu> ----- Original Message ----- From: Vicki Kalscheur To: Histonet Discussion Sent: Friday, February 11, 2011 1:21 PM Subject: Seeking equipment: leica SP 1600 Saw Microtome The company does not manufacture this saw anymore. I am interested in purchasing a USED or NEW saw if one is available. Any age or condition will be considered. We need it as soon as possible. Thank you! Vicki Vicki Kalscheur Department of Surgical Sciences School of Veterinary Medicine University of Wisconsin 2015 Linden Drive Madison, WI 53706-1102 Phone: 608-262-8534 FAX: 608-263-7930 kalschev@svm.vetmed.wisc.edu From Ronald.Houston <@t> nationwidechildrens.org Fri Feb 11 13:40:25 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Feb 11 13:40:33 2011 Subject: [Histonet] Slide Hybridizer Message-ID: If anyone has a used slide Hybridizer, e.g. Dako or Spot-Light Slide Hybridizer (Invitrogen) or StatSpin ThermoBrite* Slide Hybridizer, for sale please let me know. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rjbuesa <@t> yahoo.com Fri Feb 11 13:54:57 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 11 13:55:00 2011 Subject: [Histonet] H&E stain uneven In-Reply-To: <249316.23274.qm@web59815.mail.ac4.yahoo.com> Message-ID: <528974.51936.qm@web65713.mail.ac4.yahoo.com> Check your dewaxing ? hydrating protocol and change reagents frequently. Ren? J. --- On Fri, 2/11/11, Madeline Gi wrote: From: Madeline Gi Subject: [Histonet] H&E stain uneven To: Histonet@lists.utsouthwestern.edu Date: Friday, February 11, 2011, 1:44 PM Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why.? Can someone tell me how does a routine H&E stain unevenly?? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated?. ? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Feb 11 13:22:52 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Feb 11 14:10:17 2011 Subject: [Histonet] Decal Solution for Bone Marrows In-Reply-To: <3ACBB5D73A417547A01970CD3EB5509306EEC579@MAIL1.ecrmc.ci.el-centro.ca.us> Message-ID: Decalcifier II from Surgipath. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marcia Fisher Sent: Friday, February 11, 2011 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrows I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From PMonfils <@t> Lifespan.org Fri Feb 11 16:41:30 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Feb 11 16:41:00 2011 Subject: [Histonet] saliva for glycogen hydrolysis Message-ID: <4EBFF65383B74D49995298C4976D1D5E07CC782C@LSRIEXCH1.lsmaster.lifespan.org> Those who use human saliva rather than diastase - for how long do you apply the saliva? At what temperature? Do you cover the saliva and section with a coverslip during treatment? I used to do this many years ago, but have been using diastase for so long now that I don't remember the particulars of the saliva method, and few books seem to provide that information. Thanks. From mbrooks <@t> incytepathology.com Fri Feb 11 16:58:56 2011 From: mbrooks <@t> incytepathology.com (Matt Brooks) Date: Fri Feb 11 16:59:02 2011 Subject: [Histonet] saliva for glycogen hydrolysis In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E07CC782C@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E07CC782C@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <706224670091FE47997AEF88EFADE7CA0194ED7C@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Room Temperature for 10-15 minutes and wash well with DI H2O after. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, February 11, 2011 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saliva for glycogen hydrolysis Those who use human saliva rather than diastase - for how long do you apply the saliva? At what temperature? Do you cover the saliva and section with a coverslip during treatment? I used to do this many years ago, but have been using diastase for so long now that I don't remember the particulars of the saliva method, and few books seem to provide that information. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Feb 11 17:19:25 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Feb 11 17:19:31 2011 Subject: [Histonet] FW: CMS to Rescind Physician Signature Requirement Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E09AB96@CHEXCMS10.one.ads.che.org> Yeah!!! ________________________________ From: American Society for Clinical Pathology [mailto:ascp@site1.ascpmail.org] Sent: Friday, February 11, 2011 18:07 To: Weems, Joyce Subject: CMS to Rescind Physician Signature Requirement [http://www.ascp.org/ImageLibrary/Email-Images/ePolicyNews-Header.aspx] CMS to Rescind Physician Signature Requirement; CMS' Change in Policy Illustrates the Power of Grassroots Advocacy The American Society for Clinical Pathology (ASCP) is pleased to announce that it has learned that the Centers for Medicare and Medicaid Services (CMS) is planning to rescind its recent rule requiring a physician's signature on requisitions for laboratory tests reimbursed under the Medicare clinical laboratory fee schedule. The CMS rule proved very controversial and prompted not only the collective opposition of the entire laboratory coalition but also the American Medical Association and a number of other medical specialty societies. ASCP, in tandem with its partners in the Clinical Laboratory Coalition, mounted an aggressive campaign to protest the proposal. ASCP launched two separate advocacy campaigns on the rule. The first campaign let CMS know directly the concerns of ASCP members, while the second campaign was intended to encourage members of Congress to contact CMS in opposition to the rule. In total, ASCP members wrote more than 2,000 letters in opposition to the rule. CMS received a loud and clear message from Congress today as the agency received a letter from 89 members of the U.S. House of Representatives and another letter from more than 30 U.S. Senators. In announcing the agency's intent to withdraw the rule, it was mentioned that the agency would officially withdraw the rule well in advance of the April 1 date that the rule was supposed to go into effect. For more information on the rule, click here. ASCP will provide more information about this important development in ASCP's upcoming issue of e-Policy News. [http://www.chicago.ascp.org/pics/HologicCORP2_4c.jpg] ASCP Washington News is supported by an unrestricted grant from Hologic. Tell a friend: Not everyone receives ASCP's e-Policy News, so forward this message to your peers and co-workers. 2011 American Society for Clinical Pathology 33 West Monroe Street, Chicago, IL 60603 312.541.4999 info@ascp.org ABOUT THIS MESSAGE | You are receiving this e-mail because you have been certified by the Board of Certification (BOC) are a member of ASCP, have attended ASCP programs/meetings or made a purchase from ASCP. If you no longer wish to receive e-mails of this kind from ASCP, please login to change your email preferences or call Customer Service at 800.267.2727. This e-mail message complies with all CAN-SPAM 2004 regulations. It was sent to you by the American Society for Clinical Pathology, 33 West Monroe Street, Suite 1600, Chicago, IL 60603. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From hlukey <@t> msn.com Fri Feb 11 19:02:42 2011 From: hlukey <@t> msn.com (Hugh Luk) Date: Fri Feb 11 19:02:46 2011 Subject: [Histonet] Dr. Tony Manoukian of Maui Message-ID: Hi histonetters, Some of you may know Dr. Manoukian, then it is my sad duty to inform you of his passing. He worked as a forensic pathologist and was one heck of a great guy. For us in Hawaii, he passed too soon. http://mauinews.com/page/content.detail/id/546026/-Community-is-much-less--with-death-of-Manoukian.html?nav=10 He lived in Chicago, Minnesota, California, Grenada, and Hawaii. Thanks. From anonwums1 <@t> gmail.com Fri Feb 11 19:37:54 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Fri Feb 11 19:37:58 2011 Subject: [Histonet] Mouse CD45 staining of bone marrow Message-ID: Hi all, I have been attempting to do mouse CD45 immunofluorescence on frozen, fixed decalcified mouse sections and have an odd problem. I am using the monoclonal rat anti-mouse CD45 (clone 30F-11 from BD) and get very nice staining along the cell surface of many cells. However, only about 20% of the nucleated cells stain with CD45. By FACS, > 90% of the cells stain with CD45 using the same clone. To be fair, that is with RBC lysis, but the RBCs should not be nucleated anyhow. The antibody comes at 62.5 ug / mL, and I am using the antibody at a 1:1000 dilution in TBS-T. I found that at the recommended1:20 titer, nothing stained above background. I only began to get staining at < 1:100, suggesting to me there may have been some steric hindrance going on. Although this clone is supposed to work on FFPE sections, I have been unable to get any staining at all using even with aggressive HIER. I was wondering if anyone has IHC or IF photos of CD45 staining in mouse bone marrow so I know what it should look like. Any suggestions are welcome. Thanks, Adam The specifics: 1) Fix the bones overnight in 4% PFA at 4C 2) Decalcify in EDTA for 3 days at 4C 3) Cryoprotect in 30% sucrose overnight at 4C 4) Embed in OCT using liquid nitrogen cooled 2-methylbutane 5) Section using Cryojane tape transfer system. I have also tried this with a regular cryotome with similar results. 6) Block in 10% donkey serum, then avidin and biotin 7) Incubate with primary antibody overnight at 4C. Wash. 8) Incubate with biotinylated donkey anti-rat F(ab)2 (1 ug / mL) at room temperature for 1 hr. Wash. 9) Incubate with strepavidin Dylight 594 (1 ug / mL) at room temperature for 1 hr. Wash. 10) Mount with Prolong Gold anti-fade with DAPI From Gervaip <@t> aol.com Fri Feb 11 20:57:47 2011 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Fri Feb 11 20:57:57 2011 Subject: [Histonet] Decal Solution for Bone Marrows Message-ID: <1b197.7518967d.3a87512b@aol.com> We use the IEC from Biocare. The bone biopsies are fixed for a minimum of 2 hours and decaled in the IEC for two hours. Sections great and immunohistochemistry stains are beautiful. In a message dated 2/11/2011 2:11:23 P.M. Central Standard Time, trathborne@somerset-healthcare.com writes: Decalcifier II from Surgipath. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marcia Fisher Sent: Friday, February 11, 2011 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrows I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gervaip <@t> aol.com Fri Feb 11 21:02:53 2011 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Fri Feb 11 21:03:03 2011 Subject: [Histonet] Decal Solution for Bone Marrows Message-ID: <1b42d.4d7f814c.3a87525d@aol.com> I neglected to say that the iron stain does not work well on decaled specimens. We perform iron stains on bone marrow clots and smears . In a message dated 2/11/2011 2:11:23 P.M. Central Standard Time, trathborne@somerset-healthcare.com writes: Decalcifier II from Surgipath. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marcia Fisher Sent: Friday, February 11, 2011 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrows I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ian <@t> histobob.com Fri Feb 11 23:08:28 2011 From: Ian <@t> histobob.com (Ian) Date: Fri Feb 11 23:08:35 2011 Subject: [Histonet] Coverslipping by hand Message-ID: Remember coverslipping by hand? We do, check it out! http://www.histobob.com/?q=node/152 From lpwenk <@t> sbcglobal.net Sat Feb 12 10:01:04 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Feb 12 10:01:28 2011 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: <06A02056B3F242F6AB3D0987D9CFD4C1@HP2010> I thought I would look up the stats for the QIHC on the ASCP webpage http://www.ascp.org/PDF/ExamStat.aspx In 2010, 88 people took the exam, with 80 passing, or 91%. I then looked over all the stats from 2005-2010 (in 2005, the exam became a 50 multiple choice question exam. Previous, it had been a practical exam.) 442 people took the exam during this time, with 366 passing, or 82.8%. The pass rates during those years were: 85%, 70%, 81%, 83%, 82%, 91%. A score of 400 is required to pass. The lowest score possible is 100. The lowest scores during those years were: 220, 240, 260, 260, 300, 270. The highest score possible is 999. The highest scores during those years were: 640, 640, 680, 820, 680, 680. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Mark Tarango" Sent: Thursday, February 10, 2011 11:58 AM To: "Houston, Ronald" Cc: Subject: Re: [Histonet] QIHC > Its true, that test is too easy if you ask me. Dako handbook is all > you'll > need. > > Mark > > On Thu, Feb 10, 2011 at 5:46 AM, Houston, Ronald < > Ronald.Houston@nationwidechildrens.org> wrote: > >> Read and digest the Dako book and you will sail through the exam. For a >> "specialist type" qualification it really is pitiful. >> >> Ronnie >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Bradley >> Sent: Wednesday, February 09, 2011 4:30 PM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] QIHC >> >> Hi all >> >> I am preparing to take the QIHC exam. Does anyone have any suggestions >> on >> what I should study? What areas does the exam concentrate on and what >> research material I can use. >> >> Thanks >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> ----------------------------------------- Confidentiality Notice: >> The following mail message, including any attachments, is for the >> sole use of the intended recipient(s) and may contain confidential >> and privileged information. The recipient is responsible to >> maintain the confidentiality of this information and to use the >> information only for authorized purposes. If you are not the >> intended recipient (or authorized to receive information for the >> intended recipient), you are hereby notified that any review, use, >> disclosure, distribution, copying, printing, or action taken in >> reliance on the contents of this e-mail is strictly prohibited. If >> you have received this communication in error, please notify us >> immediately by reply e-mail and destroy all copies of the original >> message. Thank you. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sat Feb 12 10:08:33 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Feb 12 10:08:36 2011 Subject: [Histonet] 2010 HT and HTL ASCP pass rates Message-ID: <9421CAC282C844E0A23D185EA496F329@HP2010> While I was looking up the QIHC pass rates, I thought I'd let everyone know the 2010 HT and HTL pass rates. http://www.ascp.org/MainMenu/programdirectors/Laboratoryprograms/ExaminationStatistics.aspx (if you want to look up other years) HT HISTOLOGIC TECHNICIAN: 758 total took, 522 passed = 69% NAACLS students = 382 took, 294 passed = 77% If removed the first time NAACLS students from the total who took (= pass rate for the on-the-job people and the NAACLS repeaters): 376 took, 228 passed = 61%) HTL HISTOTECHNOLOGIST: 252 total took, 156 passed = 62% NAACLS students = 54 took, 38 passed = 70% If removed the first time NAACLS students from the total who took (= pass rate for the on-the-job people and the NAACLS repeaters): 198 took, 118 passed = 60% SLS SPECIALIST IN LABORATORY SAFETY: 19 total took, 13 passed = 68% Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From Rcartun <@t> harthosp.org Sat Feb 12 10:20:05 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sat Feb 12 10:20:14 2011 Subject: [Histonet] CAP question ANP.22970 In-Reply-To: References: Message-ID: <4D566CE4.7400.0077.1@harthosp.org> Here's an excellent reference - Ross JS: "Saving Lives with Accurate HER2 Testing" Am J Clin Pathol 2010;134:183-184. I quote, "A number of experts in the field have now agreed that a laboratory performing HER2 testing in the US patient population should have a HER2+ rate of approximately "16%" with a range of 12% to 20%." Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Martha Ward 2/1/2011 12:57 PM >>> We are having our inspection this spring and I am working to get all our procedures, etc. ready. I am having trouble finding benchmark information for comparison for HER2 to comply with this question - ..."the laboratory at least annually compares its patient results with published benchmarks,".... We are using the Dako Herceptest. I spoke with Dako tech services and they did not have any information. What are other labs using for a benchmark. Thanks in advance for all your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Feb 12 10:30:40 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Feb 12 10:31:20 2011 Subject: [Histonet] Decal Solution for Bone Marrows In-Reply-To: <3ACBB5D73A417547A01970CD3EB5509306EEC579@MAIL1.ecrmc.ci.el-centro.ca.us> References: <3ACBB5D73A417547A01970CD3EB5509306EEC579@MAIL1.ecrmc.ci.el-centro.ca.us> Message-ID: I use 5% formic acid, you can make it or purchase Immunocal from Decal Chemicals, it is slower than the rapid decal solutions but much better for IHC and FE (although we used to have to do Fe stains on the smears or clot because besides the decal, iron can be washed out in tissue processing). Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Fisher Sent: Friday, February 11, 2011 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrows I would be most interested in finding out what other labs are using to decal the bone marrow biopsy cores. The current decal solution we are using is too strong and affecting the Fe stains and some IHC stains. The decal solution we use for regular bone is great. Routinely we decal the core for one hour. We tried 45 minutes but the cores are still too crunchy. And I prefer not to face off the block and float on 5% HCl for 20 minutes. Thanking you in advance. M. Fisher El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. B _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Feb 12 12:23:56 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Feb 12 12:24:00 2011 Subject: [Histonet] decalcifying bone marrow biopsy specimens Message-ID: Bone marrow biopsy specimens can be decalcified with a variety of commercial and home-brewed decalcifiers. Always you have to make sure you don't leave the specimen in the decalcifier for too long. All decalcifiers leach out iron (hemosiderin). The iron stain should be done on the undecalcified clot or particle section (though I find this is almost never done). The iron stain should also be done on one of the marrow smears. Avoiding the leaching effect of fixatives is important, but even more important, the pathologist needs to look for ringed sideroblasts in the smears stained with the Perls prussian blue reaction. These usually can't be seen in sections. Bob Richmond Samurai Pathologist Knoxville TN From rsrichmond <@t> gmail.com Sat Feb 12 12:28:02 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Feb 12 12:28:06 2011 Subject: [Histonet] Re: mouse skeletal muscle frozen section prep Message-ID: Before you freeze a muscle specimen, you coat it thinly with talc, pick it up with tweezers, and bob it up and down about ten times in the freezing liquid (not directly in liquid nitrogen), then mount it in OCT or other freeze-mount medium. Correctly done, this will largely avoid bubble artifact. It takes a few times to get the knack of it - best to practice with some junk muscle (from a human amputation specimen, or from a dead animal of your choice) to get it right before you do it on an actual biopsy specimen or on valuable research material. Bob Richmond Samurai Pathologist Knoxville TN From sdeppeler <@t> ksu.edu.sa Sun Feb 13 03:03:25 2011 From: sdeppeler <@t> ksu.edu.sa (Stacy Deppeler) Date: Sun Feb 13 03:03:34 2011 Subject: [Histonet] Frozen Rat Tissue Staining Message-ID: Hi Histonetters, I've been trying to perform an immunofluorescent stain for Glutamine Synthetase on frozen rat eye sections and having very little luck. I put this down mostly to my lack of experience with frozen sections so I'm hoping I can find a little help here to guide me on where I am going horribly wrong. The problem I am having is that the retinal tissue is completely degraded by the time I am finished the stain. I have been using a staining protocol my PI gave me that his previous assistant used with good results, but for me is giving me nothing. Here's a quick summary: 1. Cut 10um sections of rat eye at -20C, mount on plus slide and air dry for 30 min. 2. Fix in -20C acetone for 10 min, air dry for 10 min. 3. 3X PBS-Tween20, 5 min. 4. 5% serum block, 10 min. 5. Primary antibody incubation, overnight at 4C. 6. 3X PBS-Tween20, 5 min. 7. Fluorophore-conjugated secondary antibody (Texas Red), 1hr. 8. 3X PBS-Tween20, 5 min. 9. IF aqueous mount with DAPI. I'm finding that the retinal tissue has already broken down significantly when I take the slides out from the overnight primary antibody incubation. My first thought is that the tissue is not fixed well enough but all my research so far tells me it should be. However, the more I read the more I'm at a loss as everyone has different opinions on fixing and air drying. The tissue did appear to be intact after cutting and fixing when observed under the microscope. Any input on my technique would be greatly appreciated as its hard to work out what you're doing wrong when you don't know what you're doing right! Thanks. -- Stacy Deppeler. (Aussie Expat) Research Assistant Department of Ophthalmology King Abdulaziz University Hospital King Saud University Riyadh, KSA From Montina.VanMeter <@t> pbrc.edu Sun Feb 13 11:21:15 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Sun Feb 13 11:21:29 2011 Subject: [Histonet] Frozen Rat Tissue Staining In-Reply-To: References: Message-ID: Stacy, How are you preserving the tissue prior to sectioning (i.e. liquid nitrogen, isopentane, etc.)? Flash freezing is essential when dealing with unfixed tissue. Tina Sent from my iPhone On Feb 13, 2011, at 3:10 AM, "Stacy Deppeler" wrote: > Hi Histonetters, > > I've been trying to perform an immunofluorescent stain for Glutamine > Synthetase on frozen rat eye sections and having very little luck. I put > this down mostly to my lack of experience with frozen sections so I'm hoping > I can find a little help here to guide me on where I am going horribly > wrong. The problem I am having is that the retinal tissue is completely > degraded by the time I am finished the stain. > > I have been using a staining protocol my PI gave me that his previous > assistant used with good results, but for me is giving me nothing. Here's a > quick summary: > > 1. Cut 10um sections of rat eye at -20C, mount on plus slide and air dry for > 30 min. > 2. Fix in -20C acetone for 10 min, air dry for 10 min. > 3. 3X PBS-Tween20, 5 min. > 4. 5% serum block, 10 min. > 5. Primary antibody incubation, overnight at 4C. > 6. 3X PBS-Tween20, 5 min. > 7. Fluorophore-conjugated secondary antibody (Texas Red), 1hr. > 8. 3X PBS-Tween20, 5 min. > 9. IF aqueous mount with DAPI. > > I'm finding that the retinal tissue has already broken down significantly > when I take the slides out from the overnight primary antibody incubation. > My first thought is that the tissue is not fixed well enough but all my > research so far tells me it should be. However, the more I read the more I'm > at a loss as everyone has different opinions on fixing and air drying. The > tissue did appear to be intact after cutting and fixing when observed under > the microscope. > > Any input on my technique would be greatly appreciated as its hard to work > out what you're doing wrong when you don't know what you're doing right! > > Thanks. > > > -- > Stacy Deppeler. (Aussie Expat) > > Research Assistant > Department of Ophthalmology > King Abdulaziz University Hospital > King Saud University > Riyadh, KSA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sun Feb 13 13:06:20 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Feb 13 13:06:24 2011 Subject: [Histonet] Re: Histonet Digest, Vol 87, Issue 24 In-Reply-To: <4d581c55.2232640a.66ab.6759SMTPIN_ADDED@mx.google.com> References: <4d581c55.2232640a.66ab.6759SMTPIN_ADDED@mx.google.com> Message-ID: Hi Stacy, I have done this on FFPE samples without terrible difficulty. I'm not sure if the I did procedure will work well on frozen tissue, but I don't see why it wouldn't. I also used HRP/DAB to visualize the reaction however a fluorescent tag should be fine. I used an antibody from AbCam (ab49873) at 1:200 with citrate buffer (pH6.0) retrieval. It might not be working for you now, but I'm sure you'll get it. Good luck, Amos On Sun, Feb 13, 2011 at 1:00 PM, wrote: > Message: 3 > Date: Sun, 13 Feb 2011 12:03:25 +0300 > From: Stacy Deppeler > Subject: [Histonet] Frozen Rat Tissue Staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Histonetters, > > I've been trying to perform an immunofluorescent stain for Glutamine > Synthetase on frozen rat eye sections and having very little luck. I put > this down mostly to my lack of experience with frozen sections so I'm > hoping > I can find a little help here to guide me on where I am going horribly > wrong. The problem I am having is that the retinal tissue is completely > degraded by the time I am finished the stain. > > I have been using a staining protocol my PI gave me that his previous > assistant used with good results, but for me is giving me nothing. Here's a > quick summary: > > 1. Cut 10um sections of rat eye at -20C, mount on plus slide and air dry > for > 30 min. > 2. Fix in -20C acetone for 10 min, air dry for 10 min. > 3. 3X PBS-Tween20, 5 min. > 4. 5% serum block, 10 min. > 5. Primary antibody incubation, overnight at 4C. > 6. 3X PBS-Tween20, 5 min. > 7. Fluorophore-conjugated secondary antibody (Texas Red), 1hr. > 8. 3X PBS-Tween20, 5 min. > 9. IF aqueous mount with DAPI. > > I'm finding that the retinal tissue has already broken down significantly > when I take the slides out from the overnight primary antibody incubation. > My first thought is that the tissue is not fixed well enough but all my > research so far tells me it should be. However, the more I read the more > I'm > at a loss as everyone has different opinions on fixing and air drying. The > tissue did appear to be intact after cutting and fixing when observed under > the microscope. > > Any input on my technique would be greatly appreciated as its hard to work > out what you're doing wrong when you don't know what you're doing right! > > Thanks. > From sdeppeler <@t> ksu.edu.sa Sun Feb 13 13:24:19 2011 From: sdeppeler <@t> ksu.edu.sa (Stacy Deppeler) Date: Sun Feb 13 13:24:29 2011 Subject: [Histonet] Frozen Rat Tissue Staining In-Reply-To: References: Message-ID: Tina, The tissue was frozen in a slurry of isopentane and dry ice. -- Stacy Deppeler. Research Assistant Department of Ophthalmology King Abdulaziz University Hospital Riyadh, KSA On 13 February 2011 20:21, Montina Van Meter wrote: > Stacy, > How are you preserving the tissue prior to sectioning (i.e. liquid > nitrogen, isopentane, etc.)? Flash freezing is essential when dealing with > unfixed tissue. > > Tina > > > > Sent from my iPhone > > On Feb 13, 2011, at 3:10 AM, "Stacy Deppeler" > wrote: > > > Hi Histonetters, > > > > I've been trying to perform an immunofluorescent stain for Glutamine > > Synthetase on frozen rat eye sections and having very little luck. I put > > this down mostly to my lack of experience with frozen sections so I'm > hoping > > I can find a little help here to guide me on where I am going horribly > > wrong. The problem I am having is that the retinal tissue is completely > > degraded by the time I am finished the stain. > > > > I have been using a staining protocol my PI gave me that his previous > > assistant used with good results, but for me is giving me nothing. Here's > a > > quick summary: > > > > 1. Cut 10um sections of rat eye at -20C, mount on plus slide and air dry > for > > 30 min. > > 2. Fix in -20C acetone for 10 min, air dry for 10 min. > > 3. 3X PBS-Tween20, 5 min. > > 4. 5% serum block, 10 min. > > 5. Primary antibody incubation, overnight at 4C. > > 6. 3X PBS-Tween20, 5 min. > > 7. Fluorophore-conjugated secondary antibody (Texas Red), 1hr. > > 8. 3X PBS-Tween20, 5 min. > > 9. IF aqueous mount with DAPI. > > > > I'm finding that the retinal tissue has already broken down significantly > > when I take the slides out from the overnight primary antibody > incubation. > > My first thought is that the tissue is not fixed well enough but all my > > research so far tells me it should be. However, the more I read the more > I'm > > at a loss as everyone has different opinions on fixing and air drying. > The > > tissue did appear to be intact after cutting and fixing when observed > under > > the microscope. > > > > Any input on my technique would be greatly appreciated as its hard to > work > > out what you're doing wrong when you don't know what you're doing right! > > > > Thanks. > > > > > > -- > > Stacy Deppeler. (Aussie Expat) > > > > Research Assistant > > Department of Ophthalmology > > King Abdulaziz University Hospital > > King Saud University > > Riyadh, KSA > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AnthonyH <@t> chw.edu.au Sun Feb 13 17:12:42 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Feb 13 17:13:00 2011 Subject: [Histonet] RE: saliva for glycogen hydrolysis In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E07CC782C@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E07CC782C@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715706395A@xmdb02.nch.kids> " and few books seem to provide that information" Probably because it is so disgusting! We used to SPIT on the sections to cover, incubate at room temp for 10 min and rinse well in water. If we had too many slides to do (I could probably SPIT on 3 slides before I dried up) I would spit into a 50ml specimen container and add PBS buffer, equal volumes, mix and pipette on to the slides and incubate for 10-15 min at room temp. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Saturday, 12 February 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saliva for glycogen hydrolysis Those who use human saliva rather than diastase - for how long do you apply the saliva? At what temperature? Do you cover the saliva and section with a coverslip during treatment? I used to do this many years ago, but have been using diastase for so long now that I don't remember the particulars of the saliva method, and few books seem to provide that information. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Tony_Reilly <@t> health.qld.gov.au Sun Feb 13 19:37:57 2011 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Sun Feb 13 19:38:36 2011 Subject: [Histonet] RE: saliva for glycogen hydrolysis In-Reply-To: <6D6BD1DE8A5571489398B392A38A715706395A@xmdb02.nch.kids> References: <4EBFF65383B74D49995298C4976D1D5E07CC782C@LSRIEXCH1.lsmaster.lifespan.org> <6D6BD1DE8A5571489398B392A38A715706395A@xmdb02.nch.kids> Message-ID: <4D591415.411C.0039.0@health.qld.gov.au> Hi Tony We used to do something similar. Spit into a test tube, add equal volume of distilled water, put on the lid and shake vigorously which makes the solution more liquid and easier to use. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> Tony Henwood 14/02/2011 9:12 am >>> " and few books seem to provide that information" Probably because it is so disgusting! We used to SPIT on the sections to cover, incubate at room temp for 10 min and rinse well in water. If we had too many slides to do (I could probably SPIT on 3 slides before I dried up) I would spit into a 50ml specimen container and add PBS buffer, equal volumes, mix and pipette on to the slides and incubate for 10-15 min at room temp. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Saturday, 12 February 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saliva for glycogen hydrolysis Those who use human saliva rather than diastase - for how long do you apply the saliva? At what temperature? Do you cover the saliva and section with a coverslip during treatment? I used to do this many years ago, but have been using diastase for so long now that I don't remember the particulars of the saliva method, and few books seem to provide that information. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From marktarango <@t> gmail.com Sun Feb 13 20:55:23 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Sun Feb 13 20:55:28 2011 Subject: [Histonet] 2010 HT and HTL ASCP pass rates In-Reply-To: <9421CAC282C844E0A23D185EA496F329@HP2010> References: <9421CAC282C844E0A23D185EA496F329@HP2010> Message-ID: Since you mentioned the SLS exam, it might be important to note this quote taken from the ASCP webpage for anyone interested: http://www.ascp.org/FunctionalNavigation/certification/GetCertified/SpecialistCertification.aspx *"The certification, Specialist in Laboratory Safety (SLS), will be changed to a qualification effective January 1, 2012. The last certification examination for SLS(ASCP) will be administered December 30, 2011, with a deadline date of August 1, 2011 for receipt of application forms. As a qualification, the eligibility requirements have been revised and the examination will be administered online as a 50-item examination. Individuals currently SLS(ASCP) certified will retain their certification and continue to participate in the Certification Maintenance Program (CMP)." * Mark Tarango HT(ASCP)QIHC, SLS On Sat, Feb 12, 2011 at 8:08 AM, Lee & Peggy Wenk wrote: > While I was looking up the QIHC pass rates, I thought I'd let everyone know > the 2010 HT and HTL pass rates. > > http://www.ascp.org/MainMenu/programdirectors/Laboratoryprograms/ExaminationStatistics.aspx > (if you want to look up other years) > > HT HISTOLOGIC TECHNICIAN: > 758 total took, 522 passed = 69% > NAACLS students = 382 took, 294 passed = 77% > If removed the first time NAACLS students from the total who took (= pass > rate for the on-the-job people and the NAACLS repeaters): 376 took, 228 > passed = 61%) > > HTL HISTOTECHNOLOGIST: > 252 total took, 156 passed = 62% > NAACLS students = 54 took, 38 passed = 70% > If removed the first time NAACLS students from the total who took (= pass > rate for the on-the-job people and the NAACLS repeaters): 198 took, 118 > passed = 60% > > SLS SPECIALIST IN LABORATORY SAFETY: > 19 total took, 13 passed = 68% > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BMolinari <@t> heart.thi.tmc.edu Mon Feb 14 05:54:11 2011 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Feb 14 05:58:44 2011 Subject: [Histonet] H&E stain uneven In-Reply-To: <249316.23274.qm@web59815.mail.ac4.yahoo.com> References: <249316.23274.qm@web59815.mail.ac4.yahoo.com> Message-ID: I had this problem awhile back. Did you change your solvent for deparaffinization? I changed from xylene to another solvent and realized I had to extend the time my slides were in it. Betsy Molinari HT(ASCP) Texas Heart Institue Cardiovascular Pathology 1101 Bates Street Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeline Gi Sent: Friday, February 11, 2011 12:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E stain uneven Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why.? Can someone tell me how does a routine H&E stain unevenly?? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated?. ? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From luciana.bastos <@t> hotmail.com Mon Feb 14 07:06:19 2011 From: luciana.bastos <@t> hotmail.com (Luciana Bastos) Date: Mon Feb 14 07:06:25 2011 Subject: [Histonet] processing histogel Message-ID: Hello, We are working with cell culture embedded into three dimensional (3D) of collagen gel type I. The samples were fixed in 10% formalin for 24 h. Even after fixation, the gel used in our preparation was too soft to be embedded directly in paraffin. To circumvent this problem, we wold like to dehydrated and embedded the samples in Histogel (Perk Scientific Inc., Devon, PA, USA).11 V.M. Freitas and R.G. Jaeger, The effect of laminin and its peptide SIKVAV on a human salivary gland adenoid cystic carcinoma cell line, Virchows Arch 441 (6) (2002), pp. 569?576. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (12)11 However, we need some help to how dehydrated the collagen gel and the histogel (solutions, concetration, time, ect.) toparaffin-embedded and stained by haematoxylin?eosin (H&E). Thank you, Luciana From JWeems <@t> sjha.org Mon Feb 14 09:07:44 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Feb 14 09:07:50 2011 Subject: [Histonet] 2010 HT and HTL ASCP pass rates In-Reply-To: <9421CAC282C844E0A23D185EA496F329@HP2010> References: <9421CAC282C844E0A23D185EA496F329@HP2010> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E09AC5E@CHEXCMS10.one.ads.che.org> Thanks, Peggy, for keeping us informed. You're a jewel! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Saturday, February 12, 2011 11:09 To: Histonet Subject: [Histonet] 2010 HT and HTL ASCP pass rates While I was looking up the QIHC pass rates, I thought I'd let everyone know the 2010 HT and HTL pass rates. http://www.ascp.org/MainMenu/programdirectors/Laboratoryprograms/ExaminationStatistics.aspx (if you want to look up other years) HT HISTOLOGIC TECHNICIAN: 758 total took, 522 passed = 69% NAACLS students = 382 took, 294 passed = 77% If removed the first time NAACLS students from the total who took (= pass rate for the on-the-job people and the NAACLS repeaters): 376 took, 228 passed = 61%) HTL HISTOTECHNOLOGIST: 252 total took, 156 passed = 62% NAACLS students = 54 took, 38 passed = 70% If removed the first time NAACLS students from the total who took (= pass rate for the on-the-job people and the NAACLS repeaters): 198 took, 118 passed = 60% SLS SPECIALIST IN LABORATORY SAFETY: 19 total took, 13 passed = 68% Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From alisha <@t> ka-recruiting.com Mon Feb 14 09:09:50 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Mon Feb 14 09:09:20 2011 Subject: [Histonet] Muscle and Nerve Technician Job Opportunity Message-ID: <1174090183.1297696190283.JavaMail.cfservice@SL4APP4> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must * Muscle/Nerve Technician - Must have muscle biopsy experience They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From mollie <@t> phylogenyinc.com Mon Feb 14 09:21:30 2011 From: mollie <@t> phylogenyinc.com (Mollie Hannon) Date: Mon Feb 14 09:22:19 2011 Subject: [Histonet] Part-time Histotech Position in Columbus, Ohio Message-ID: <08B339BE-7303-4987-83CC-199A47728F2F@phylogenyinc.com> Small Columbus-based biotech company is searching for an experienced, fun loving histotech. The ideal candidate should live in Central Ohio, be personable, able to adjust quickly to a fast growing company and the responsibilities that come with helping a company grow. Skills & responsibilities should include, but are not limited to: -Routine cutting and embedding tissue -Staining Slides -Project management This position is part-time, with a flexible schedule & competitive compensation. Please email resume and references to mollie@phylogenyinc.com From relia1 <@t> earthlink.net Mon Feb 14 09:22:40 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Feb 14 09:22:49 2011 Subject: [Histonet] RELIA Histology Careers Bulletin 2-14-2011 Happy Valentine's Day Message-ID: <2562323FCE0A468E9DC87B4C66D64C59@ownerf1abaad51> Hi Histonetters!! Happy Valentines Day! Right Place, Right Time, Right Opportunity. Sometimes your next career move is just a matter of being in the right place at the right time and ready for the next opportunity. Odds are that if you are contemplating a job change for whatever reason ? better compensation, a more desirable location or more challenging work, you don?t have the time to do a job search. Let me help you with that. I have clients located throughout the country. All of the jobs that I represent are full time permanent positions with excellent compensation, benefits and relocation/sign-on bonuses. If you are ASCP certified or eligible my clients are interested in speaking with you! I will assist you with your resume, coordination of interviews and coaching throughout the process. Remember my services are FREE of charge to you. Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions or a position like anyone of these but in another area either way ? give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1@earthlink.net and let?s discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! *These are our brand new opportunities* HISTOLOGY MANAGEMENT *NC ? Histology Lab Manager ? Western NC* NH- Histology Supervisor - Manchester, NH *ME ? Histology Supervisor ? Scarborough, ME* CA - Histology Lab Manager ? Central Valley of CA *CO ? Histology Manager ? Colorado Springs* LA - Histology Supervisor ? Baton Rouge, LA *WI ? IHC Specialist/Supervisor ?Milwaukee* HISTOTECHS *IL ? Chicago Histology Training Specialist* *LA ? near Baton Rouge ? Night Shift Histotech* *TX ? Austin ? Histotechnician/Histotechnologist* *MA ? North of Boston dermpath exper a plus.* TN ? Nashville Histotechnician. NY ? Long Island Histotechs NYS license req. *NY ? Long Island Electron Microscopy Specialist NYS license req.* *NY-Orange/Rockland County Lead Tech ? days NYS license req. *WI ? Lead Histotech/Immunohistochemistry Specialist* Remember if nothing sounds interesting on my current list you can pass it on to your friends and wait for the next one OR give me a call or shoot me an e-mail telling me what you are looking for in your next opportunity. Remember timing is everything. **Also, if you know someone who might be interested I offer a $500 referral bonus** There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 9 years I have dedicated my practice solely to placing histology professionals like you. Thanks ? Pam 866-60-RELIA (866-607-3542) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From sfeher <@t> CMC-NH.ORG Mon Feb 14 09:24:38 2011 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Feb 14 09:24:43 2011 Subject: [Histonet] cassette slide/labeling In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BA2D6B21@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BA2D6B21@EXCHANGESB> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24669A3F@exchange.cmc-nh.org> We use Leica's IPC and IPS system and are very pleased with it. These labelers can be interfaced with any LIS system so that to the system they appear as just another printer. We also use the IPS to print our cytology slides that we use with the ThinPrep Imager. We are also looking into a new product that Leica will be fielding soon called Leica Cerebro specimen tracking system. This system is sort of a middle ware that will interface with the LIS to enable bar code scanning instead of manual entry for virtually every step in the process. That includes everything from accessions to final check out. If we end up with this system, I will let you know how we like it. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Wednesday, February 09, 2011 9:45 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette slide/labeling We are looking to get a bar-coded system in our laboratory. We currently hand write all cassettes and slides. What labelers do you have and do you like them? Also does anyone have the Ventana Vantage system? If so, would you recommend Vantage? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Mon Feb 14 09:26:27 2011 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Feb 14 09:26:31 2011 Subject: [Histonet] Artisan In-Reply-To: <4D53F1CD.5D38.00EF.0@swmail.sw.org> References: <4D53F1CD.5D38.00EF.0@swmail.sw.org> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24669A40@exchange.cmc-nh.org> We have had no issues with this. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Thursday, February 10, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Cc: Patricia Webster Subject: [Histonet] Artisan Has anyone had background issues with the Gram stain on the Dako Artisan Link? If so - how was it resolved? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 From sfeher <@t> CMC-NH.ORG Mon Feb 14 09:31:26 2011 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Feb 14 09:31:28 2011 Subject: [Histonet] cell block fixation In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE2D4@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE2D4@dhmail.dhorg.org> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24669A41@exchange.cmc-nh.org> Try a 50/50 mix of formalin and 95% alcohol. Have your prep techs add about 5 mL of this mixture and a drop of albumin (we use the bovine albumin from the blood bank but any albumin will do) to the cell block contents. Mix well and centrifuge. The button should be well formed. Take care not to add too much albumin or the tissue will be brittle and difficult to cut. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Wednesday, February 09, 2011 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block fixation We recently switched vendors for our formalin and while we have not experienced any difference with our surgical specimens, our cell blocks from body fluids have been giving us a great deal of trouble. The button that we get never seems to harden, leaving it sort of gelatinous, even if left to sit in formalin for days. We are able to get sections off of these cell blocks, however, the slides are blank by the end of the staining process. This is only a recent development that seems to coincide with the time we switched formalin vendors and it only happens with body fluid specimens (FNA specimens don't seem to give us as much trouble). The composition of the formalin is almost identical between vendors. Can anyone help me explain why this might be happening? Thank you in advance, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Mon Feb 14 09:41:06 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Feb 14 09:41:09 2011 Subject: [Histonet] cell block fixation In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC24669A41@exchange.cmc-nh.org> References: <38A56C4F4630D348A50B3720409270870E0FE2D4@dhmail.dhorg.org> <0908FC0A43B87A4FB60EDCCA06AABC24669A41@exchange.cmc-nh.org> Message-ID: You could also try to make them with plasma and thrombin...Unless you are trying to do IHC with them, it will work fine. The IHC might become a problem because of the plasma "donor's" antigens might get in your way? After you make the "booger" just fix and it should be fine. Another way I have tried is to used an agar solution to make the pellet. Good Luck! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, February 14, 2011 9:31 AM To: Hutton, Allison; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cell block fixation Try a 50/50 mix of formalin and 95% alcohol. Have your prep techs add about 5 mL of this mixture and a drop of albumin (we use the bovine albumin from the blood bank but any albumin will do) to the cell block contents. Mix well and centrifuge. The button should be well formed. Take care not to add too much albumin or the tissue will be brittle and difficult to cut. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Wednesday, February 09, 2011 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block fixation We recently switched vendors for our formalin and while we have not experienced any difference with our surgical specimens, our cell blocks from body fluids have been giving us a great deal of trouble. The button that we get never seems to harden, leaving it sort of gelatinous, even if left to sit in formalin for days. We are able to get sections off of these cell blocks, however, the slides are blank by the end of the staining process. This is only a recent development that seems to coincide with the time we switched formalin vendors and it only happens with body fluid specimens (FNA specimens don't seem to give us as much trouble). The composition of the formalin is almost identical between vendors. Can anyone help me explain why this might be happening? Thank you in advance, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ibryan <@t> CMC-NH.ORG Mon Feb 14 09:53:30 2011 From: ibryan <@t> CMC-NH.ORG (Bryan, Isabelle) Date: Mon Feb 14 09:53:33 2011 Subject: [Histonet] RE: H&E stain uneven In-Reply-To: <2322245927580806085035176621941@psmtp.com> References: <2322245927580806085035176621941@psmtp.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF1913CE1C36@exchange.cmc-nh.org> Patchy/Irregular H&E staining *Water left in tissue, incomplete drying: make sure to completely dry your slides. *Residual wax preventing penetration of aqueous and alcoholic dye solutions leaving areas totally devoid of stain or Xylene allowed to accumulate at top of slide during dewaxing preventing staining by aqueous solution: replace xylene bath if contains traces of water, prolong xylene treatment, keep correct levels in baths. Uneven/Poor chromatin *Water or formalin contamination in infiltrating paraffin *Contamination of reagents on processor: check equipment for malfunction, replace reagents in processor *Absorption of atmospheric water by the dehydrating alcohols: check humidity levels in lab Isabelle Bryan Catholic Medical Center, Manchester NH original message: Message: 1 Date: Fri, 11 Feb 2011 10:44:15 -0800 (PST) From: Madeline Gi Subject: [Histonet] H&E stain uneven To: Histonet@lists.utsouthwestern.edu Message-ID: <249316.23274.qm@web59815.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why.?? Can someone tell me how does a routine H&E stain unevenly??? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated???. ?? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com From sgoebel <@t> mirnarx.com Mon Feb 14 10:00:36 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Feb 14 10:00:44 2011 Subject: [Histonet] Chloroform Message-ID: Does anyone know of a special or IHC stain to detect chloroform? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From akemiat3377 <@t> yahoo.com Mon Feb 14 10:11:10 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Mon Feb 14 10:12:19 2011 Subject: [Histonet] Previously Cut Slides for FS on Muscle Message-ID: <86E3CAFE-FC14-4C90-B8D6-C285F3CE2EDE@yahoo.com> Happy Valentine's week! I haven't done histochemistry on muscle biopsies since the early 80's. I am using a isopentane/ liquid nitrogen method to freeze the muscle biopsy, so that part is covered. I cut several practice slides on a post the previous night to work up my stain protocol's. I want to optimize our stains so I cut around 25 slides at a time. I put them in a plastic slide box, wrapped it in aluminum foil, and then put it in the -80 freezer till the next day. Sounded good, but the tissue dried out, and had artifact. Obviously -80 was not good for the slides. Can I cut the slides and leave them at room temperature till the next day, or should I put them in a -20 freezer? I am curious how long slides which have been cut on muscle Bx's will last at RT. Anyone know, or have any suggestions? Thank you in advance for your assistance, Akemi Allison BS, HT (ASCP) HTL From rjbuesa <@t> yahoo.com Mon Feb 14 10:24:48 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 14 10:24:53 2011 Subject: [Histonet] Chloroform In-Reply-To: Message-ID: <292279.46507.qm@web65702.mail.ac4.yahoo.com> Chloroform cannot be detected with histology or IHC methods. It will require a chemical test. Ren? J. --- On Mon, 2/14/11, sgoebel@mirnarx.com wrote: From: sgoebel@mirnarx.com Subject: [Histonet] Chloroform To: histonet@lists.utsouthwestern.edu Date: Monday, February 14, 2011, 11:00 AM Does anyone know of a special or IHC stain to detect chloroform? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From espenceri <@t> yahoo.com Mon Feb 14 10:38:12 2011 From: espenceri <@t> yahoo.com (Elizabeth Spenceri) Date: Mon Feb 14 10:38:16 2011 Subject: [Histonet] RE: H&E stain uneven In-Reply-To: <73A7ED895EE0C24D9267ED814911DF1913CE1C36@exchange.cmc-nh.org> Message-ID: <684576.59886.qm@web121408.mail.ne1.yahoo.com> What about uneven H&E staining on frozen sections (where paraffin is not involved?) -Beth --- On Mon, 2/14/11, Bryan, Isabelle wrote: From: Bryan, Isabelle Subject: [Histonet] RE: H&E stain uneven To: histonet@lists.utsouthwestern.edu, madelinegi@yahoo.com Date: Monday, February 14, 2011, 9:53 AM Patchy/Irregular H&E staining??? *Water left in tissue, incomplete drying: make sure to completely dry your slides. *Residual wax preventing penetration of aqueous and alcoholic dye solutions leaving areas totally devoid of stain or Xylene allowed to accumulate at top of slide during dewaxing preventing staining by aqueous solution: replace xylene bath if contains traces of water, prolong xylene treatment, keep correct levels in baths. Uneven/Poor chromatin??? *Water or formalin contamination in infiltrating paraffin *Contamination of reagents on processor: check equipment for malfunction, replace reagents in processor *Absorption of atmospheric water by the dehydrating alcohols: check humidity levels in lab Isabelle Bryan Catholic Medical Center, Manchester NH original message: Message: 1 Date: Fri, 11 Feb 2011 10:44:15 -0800 (PST) From: Madeline Gi Subject: [Histonet] H&E stain uneven To: Histonet@lists.utsouthwestern.edu Message-ID: <249316.23274.qm@web59815.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why.?? Can someone tell me how does a routine H&E stain unevenly??? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated???. ?? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Scott <@t> ahss.org Mon Feb 14 10:49:37 2011 From: Lynn.Scott <@t> ahss.org (Scott, Lynn M.) Date: Mon Feb 14 10:49:42 2011 Subject: [Histonet] RE: H&E stain uneven In-Reply-To: <684576.59886.qm@web121408.mail.ne1.yahoo.com> References: <73A7ED895EE0C24D9267ED814911DF1913CE1C36@exchange.cmc-nh.org> <684576.59886.qm@web121408.mail.ne1.yahoo.com> Message-ID: <73ABF8264B77274B81BF478ADBE571AC02B6489879@LKMVXCHMB24.ADVENTISTCORP.NET> I had this happen before and our issue was that the stain froze in shipment. Change stainer with a lot number that was not shipped in cold weather and see if it helps. Lynn M. Scott, MLT, HT, (ASCP) QIHC Adventist Lab Partners Regional Supervisor, Pathology (630) 856-7859 Lynn.Scott@ahss.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Spenceri Sent: Monday, February 14, 2011 10:38 AM To: histonet@lists.utsouthwestern.edu; madelinegi@yahoo.com; IsabelleBryan Subject: Re: [Histonet] RE: H&E stain uneven What about uneven H&E staining on frozen sections (where paraffin is not involved?) -Beth --- On Mon, 2/14/11, Bryan, Isabelle wrote: From: Bryan, Isabelle Subject: [Histonet] RE: H&E stain uneven To: histonet@lists.utsouthwestern.edu, madelinegi@yahoo.com Date: Monday, February 14, 2011, 9:53 AM Patchy/Irregular H&E staining??? *Water left in tissue, incomplete drying: make sure to completely dry your slides. *Residual wax preventing penetration of aqueous and alcoholic dye solutions leaving areas totally devoid of stain or Xylene allowed to accumulate at top of slide during dewaxing preventing staining by aqueous solution: replace xylene bath if contains traces of water, prolong xylene treatment, keep correct levels in baths. Uneven/Poor chromatin??? *Water or formalin contamination in infiltrating paraffin *Contamination of reagents on processor: check equipment for malfunction, replace reagents in processor *Absorption of atmospheric water by the dehydrating alcohols: check humidity levels in lab Isabelle Bryan Catholic Medical Center, Manchester NH original message: Message: 1 Date: Fri, 11 Feb 2011 10:44:15 -0800 (PST) From: Madeline Gi Subject: [Histonet] H&E stain uneven To: Histonet@lists.utsouthwestern.edu Message-ID: <249316.23274.qm@web59815.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why.?? Can someone tell me how does a routine H&E stain unevenly??? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated???. ?? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Mon Feb 14 10:50:24 2011 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Mon Feb 14 10:50:28 2011 Subject: [Histonet] Previously Cut Slides for FS on Muscle In-Reply-To: <86E3CAFE-FC14-4C90-B8D6-C285F3CE2EDE@yahoo.com> References: <86E3CAFE-FC14-4C90-B8D6-C285F3CE2EDE@yahoo.com> Message-ID: <2108AECB05DBFF48A9C436A79215574006207CD6@UWHC-MAIL03.uwhis.hosp.wisc.edu> Did you run an H&E or any other stain on the case before you stored the slides at -80? The muscle tissue very likely was dried out and had artifact to begin with even before you stored the sections/slides in the freezer. I have stored muscle sections for days/weeks/months at -80 without any adverse staining issues. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Monday, February 14, 2011 10:11 AM To: histonet Cc: akallison@chla.usc.edu Subject: [Histonet] Previously Cut Slides for FS on Muscle Happy Valentine's week! I haven't done histochemistry on muscle biopsies since the early 80's. I am using a isopentane/ liquid nitrogen method to freeze the muscle biopsy, so that part is covered. I cut several practice slides on a post the previous night to work up my stain protocol's. I want to optimize our stains so I cut around 25 slides at a time. I put them in a plastic slide box, wrapped it in aluminum foil, and then put it in the -80 freezer till the next day. Sounded good, but the tissue dried out, and had artifact. Obviously -80 was not good for the slides. Can I cut the slides and leave them at room temperature till the next day, or should I put them in a -20 freezer? I am curious how long slides which have been cut on muscle Bx's will last at RT. Anyone know, or have any suggestions? Thank you in advance for your assistance, Akemi Allison BS, HT (ASCP) HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Mon Feb 14 11:09:19 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Feb 14 11:09:25 2011 Subject: [Histonet] Re: saliva for glycogen hydrolysis Message-ID: Here's a truly hi-tech suggestion for doing PAS-diastase stains by the spit method (which by the way is still how it's done by the small pathology services I work on): You can produce copious quantities of saliva by the simple expedient of chewing on a rubber band for a couple of minutes. This used to be the technique used to obtain saliva samples for determination of ABH substance secretor status in the blood bank. (I belong to that elite 20% of donors who are non-secretors, and Lewis-a positive to prove it.) Bob Richmond Samurai pathologist and occasional sialogogue Knoxville TN From rjbuesa <@t> yahoo.com Mon Feb 14 11:30:36 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 14 11:30:40 2011 Subject: [Histonet] RE: H&E stain uneven In-Reply-To: <684576.59886.qm@web121408.mail.ne1.yahoo.com> Message-ID: <996862.2456.qm@web65707.mail.ac4.yahoo.com> What about when you do not completely eliminate the OCT? Ren? J. --- On Mon, 2/14/11, Elizabeth Spenceri wrote: From: Elizabeth Spenceri Subject: Re: [Histonet] RE: H&E stain uneven To: histonet@lists.utsouthwestern.edu, madelinegi@yahoo.com, "IsabelleBryan" Date: Monday, February 14, 2011, 11:38 AM What about uneven H&E staining on frozen sections (where paraffin is not involved?) -Beth --- On Mon, 2/14/11, Bryan, Isabelle wrote: From: Bryan, Isabelle Subject: [Histonet] RE: H&E stain uneven To: histonet@lists.utsouthwestern.edu, madelinegi@yahoo.com Date: Monday, February 14, 2011, 9:53 AM Patchy/Irregular H&E staining??? *Water left in tissue, incomplete drying: make sure to completely dry your slides. *Residual wax preventing penetration of aqueous and alcoholic dye solutions leaving areas totally devoid of stain or Xylene allowed to accumulate at top of slide during dewaxing preventing staining by aqueous solution: replace xylene bath if contains traces of water, prolong xylene treatment, keep correct levels in baths. Uneven/Poor chromatin??? *Water or formalin contamination in infiltrating paraffin *Contamination of reagents on processor: check equipment for malfunction, replace reagents in processor *Absorption of atmospheric water by the dehydrating alcohols: check humidity levels in lab Isabelle Bryan Catholic Medical Center, Manchester NH original message: Message: 1 Date: Fri, 11 Feb 2011 10:44:15 -0800 (PST) From: Madeline Gi Subject: [Histonet] H&E stain uneven To: Histonet@lists.utsouthwestern.edu Message-ID: <249316.23274.qm@web59815.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why.?? Can someone tell me how does a routine H&E stain unevenly??? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated???. ?? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Don't get soaked. Take a quick peek at the forecast with the Yahoo! Search weather shortcut. http://tools.search.yahoo.com/shortcuts/#loc_weather From rjbuesa <@t> yahoo.com Mon Feb 14 11:32:30 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 14 11:32:33 2011 Subject: [Histonet] Re: saliva for glycogen hydrolysis In-Reply-To: Message-ID: <750626.86752.qm@web65709.mail.ac4.yahoo.com> It is cute the "sialogogue" term! Ren? J. --- On Mon, 2/14/11, Robert Richmond wrote: From: Robert Richmond Subject: [Histonet] Re: saliva for glycogen hydrolysis To: histonet@lists.utsouthwestern.edu Date: Monday, February 14, 2011, 12:09 PM Here's a truly hi-tech suggestion for doing PAS-diastase stains by the spit method (which by the way is still how it's done by the small pathology services I work on): You can produce copious quantities of saliva by the simple expedient of chewing on a rubber band for a couple of minutes. This used to be the technique used to obtain saliva samples for determination of ABH substance secretor status in the blood bank. (I belong to that elite 20% of donors who are non-secretors, and Lewis-a positive to prove it.) Bob Richmond Samurai pathologist and occasional sialogogue Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. http://tools.search.yahoo.com/toolbar/features/mail/ From TJJ <@t> stowers.org Mon Feb 14 11:52:23 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Feb 14 11:52:33 2011 Subject: [Histonet] Re: Mouse CD45 staining of bone marrow Message-ID: Dear Adam, Thank you for your detailed email regarding your problem with CD45 immunostaining! That helps tremendously know where to start in a reply. I have cc'd the Frozen section CD marker guru on this, so expect an equally detailed response to this in the near future. She'll be able to tell you how to proceed here, but I suspect your problem is aldehyde fixation. The amount of time the cells are fixed for flow cytometry is very minimal. Quite different from the overnight treatment your protocol uses. Gayle will likely tell you to use unfixed, undecalcified Cryojane sectioned bone sections that are post-sectioning fixed in Acetone/Ethanol 3:1. First I need to know if the immunostaining protocol you are using is currently providing nice strong immunostaining for other markers. In other words, is the detection you are using a sound one at those conditions? If no, then optimize your detection system first. If yes, then read on. If you are liking the morphology of the fixed and cryopreserved bone (and who wouldn't?) then you can perhaps try using Zinc Formalin as a fixative prior to sucrose cryoprotection and see if that works. Some CD markers work only with Zinc Tris buffer (Beckstead), that contains no aldehyde. You can find tons of information on this by doing a histonet or google search. Fix the tissues with the Zinc Tris, decalcify in EDTA, section, and then do what you usually do. For the samples you already have available, maybe try using some gentle HIER with citrate pH 6.0 or a higher pH, 60 degrees C for 10-20 minutes to see if you can recover some of the antigens. Yes, you can do AR on frozen sections on fixed tissues. Ok, start there. This is just a mere appetizer compared to the full course meal you are about to get. Enjoy, and good luck! ~Teri Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From Nalini.Makhijani <@t> va.gov Mon Feb 14 11:59:09 2011 From: Nalini.Makhijani <@t> va.gov (Makhijani, Nalini S) Date: Mon Feb 14 11:59:17 2011 Subject: [Histonet] processing histogel In-Reply-To: References: Message-ID: <715FA772CEA81A47B1A762AB6E45123A08B56631@VHAV22MSGA3.v22.med.va.gov> You could try asking the authors of the paper for their protocol. Nalini -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luciana Bastos Sent: Monday, February 14, 2011 5:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing histogel Hello, We are working with cell culture embedded into three dimensional (3D) of collagen gel type I. The samples were fixed in 10% formalin for 24 h. Even after fixation, the gel used in our preparation was too soft to be embedded directly in paraffin. To circumvent this problem, we wold like to dehydrated and embedded the samples in Histogel (Perk Scientific Inc., Devon, PA, USA).11 V.M. Freitas and R.G. Jaeger, The effect of laminin and its peptide SIKVAV on a human salivary gland adenoid cystic carcinoma cell line, Virchows Arch 441 (6) (2002), pp. 569-576. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (12)11 However, we need some help to how dehydrated the collagen gel and the histogel (solutions, concetration, time, ect.) toparaffin-embedded and stained by haematoxylin-eosin (H&E). Thank you, Luciana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> pathologyarts.com Mon Feb 14 12:05:17 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Mon Feb 14 12:05:21 2011 Subject: [Histonet] unsubscribe Message-ID: <005e01cbcc71$bc9cbb30$35d63190$@tague@pathologyarts.com> Curt Tague CEO, Pathology Arts 951.270.0605 Bus. 951.270.0675 Fax 909.266.7552 Cell From POWELL_SA <@t> mercer.edu Mon Feb 14 13:05:46 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Feb 14 13:05:52 2011 Subject: [Histonet] Weekly reminder Message-ID: <9BF995BC0E47744E9673A41486E24EE22DBB32E98A@MERCERMAIL.MercerU.local> Reminder to all Georgia, Alabama, ALL histotechs, The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge. The deadline for making hotel reservations is March 1, 2011 so that gives you a month to make your plans to attend, don't delay. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The complete program can be downloaded from our website at this link: www.histosearch.com/gsh> then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form. The Vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance. Come TRANSFORM yourselves. Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From madelinegi <@t> yahoo.com Mon Feb 14 13:28:28 2011 From: madelinegi <@t> yahoo.com (Madeline Gi) Date: Mon Feb 14 13:28:32 2011 Subject: [Histonet] H&E stain uneven Message-ID: <487903.41084.qm@web59804.mail.ac4.yahoo.com> Everyone thank you all so much for your helpful responses, it was my oven I called the company they fixed it everything?is?back to normal..... Thank goodness and thanks again great?job virtual team?Lol Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com From laurie.colbert <@t> huntingtonhospital.com Mon Feb 14 13:46:40 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Feb 14 13:46:44 2011 Subject: [Histonet] RE: H&E stain uneven In-Reply-To: <684576.59886.qm@web121408.mail.ne1.yahoo.com> References: <73A7ED895EE0C24D9267ED814911DF1913CE1C36@exchange.cmc-nh.org> <684576.59886.qm@web121408.mail.ne1.yahoo.com> Message-ID: <57BE698966D5C54EAE8612E8941D768301268F83@EXCHANGE3.huntingtonhospital.com> Air bubble under the tissue section. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Spenceri Sent: Monday, February 14, 2011 8:38 AM To: histonet@lists.utsouthwestern.edu; madelinegi@yahoo.com; IsabelleBryan Subject: Re: [Histonet] RE: H&E stain uneven What about uneven H&E staining on frozen sections (where paraffin is not involved?) -Beth --- On Mon, 2/14/11, Bryan, Isabelle wrote: From: Bryan, Isabelle Subject: [Histonet] RE: H&E stain uneven To: histonet@lists.utsouthwestern.edu, madelinegi@yahoo.com Date: Monday, February 14, 2011, 9:53 AM Patchy/Irregular H&E staining??? *Water left in tissue, incomplete drying: make sure to completely dry your slides. *Residual wax preventing penetration of aqueous and alcoholic dye solutions leaving areas totally devoid of stain or Xylene allowed to accumulate at top of slide during dewaxing preventing staining by aqueous solution: replace xylene bath if contains traces of water, prolong xylene treatment, keep correct levels in baths. Uneven/Poor chromatin??? *Water or formalin contamination in infiltrating paraffin *Contamination of reagents on processor: check equipment for malfunction, replace reagents in processor *Absorption of atmospheric water by the dehydrating alcohols: check humidity levels in lab Isabelle Bryan Catholic Medical Center, Manchester NH original message: Message: 1 Date: Fri, 11 Feb 2011 10:44:15 -0800 (PST) From: Madeline Gi Subject: [Histonet] H&E stain uneven To: Histonet@lists.utsouthwestern.edu Message-ID: <249316.23274.qm@web59815.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why.?? Can someone tell me how does a routine H&E stain unevenly??? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated???. ?? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Feb 14 14:34:32 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Feb 14 14:34:36 2011 Subject: [Histonet] processing histogel In-Reply-To: Message-ID: Luciana You process histogel samples essentially the same way you process regular tissue samples. I'm assuming you will be dealing with a about a 1 cm cube sample that you want embedded into the histogel and then you would then slice the histogel cube (with the collagen construct) into 3 mm sections and process to paraffin. You will need some type of mold to make the histogel cube. I would use a peel-away mold that is a bit bigger than your construct. If your construct is smaller than the 1cm cube then you might be able to get away with a cryomold, or embedding mole. I would basically use the histogel as the manufacture states, melt to the correct temp, pour a bit of it into the mold, place your fixed construct on top of it and fill up the mold with more histogel, let solidify and then place the entire sample in 10% NBF and then process to paraffin. We have dealt with a lot of different constructs and sometimes you just have work with them a bit to get what you need out of the paraffin sections. I would not waste your cultured samples right away but work out your technique on the construct alone initially. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luciana Bastos Sent: Monday, February 14, 2011 6:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing histogel Hello, We are working with cell culture embedded into three dimensional (3D) of collagen gel type I. The samples were fixed in 10% formalin for 24 h. Even after fixation, the gel used in our preparation was too soft to be embedded directly in paraffin. To circumvent this problem, we wold like to dehydrated and embedded the samples in Histogel (Perk Scientific Inc., Devon, PA, USA).11 V.M. Freitas and R.G. Jaeger, The effect of laminin and its peptide SIKVAV on a human salivary gland adenoid cystic carcinoma cell line, Virchows Arch 441 (6) (2002), pp. 569-576. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (12)11 However, we need some help to how dehydrated the collagen gel and the histogel (solutions, concetration, time, ect.) toparaffin-embedded and stained by haematoxylin-eosin (H&E). Thank you, Luciana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> nyumc.org Mon Feb 14 15:45:20 2011 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Mon Feb 14 15:45:24 2011 Subject: [Histonet] Freezing Mouse Eyes Message-ID: Any guidance, suggestions advice on best methods or procedures would be greatly appreciated! Thanks in advance Luis Chiriboga Experimental Pathology Core Laboratory New York University School of Medicine ------------------------------------------------------------
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================================= 
From Heidi.Hawthorne <@t> onassignment.com  Mon Feb 14 17:27:44 2011
From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne)
Date: Mon Feb 14 17:27:52 2011
Subject: [Histonet] Histotech candidates in the San Francisco Bay Area
Message-ID: <26C4A3B38503BC4CBBF4D986C3BC9B830904D1160C@oasslcexm01.oaifield.onasgn.com>

Candidates Announced!

I am pleased to announce that we have a great pool of qualified Histotechs in the San Francisco Bay Area!  Through our recent recruiting efforts for a local hospital, we have identified several great Histotechs that are available for full-time, part-time and oncall positions.  Our candidates have a wealth of experience in hospitals, research facilities and a variety of lab settings.

Contact me today if you have an open position or know of someone that does!

Heidi Hawthorne
Sr. Account Executive
On Assignment, Inc.
t: (510) 663-8622
c: (510) 435-7326
f: (866) 741-0805
Heidi.Hawthorne@onassignment.com
www.onassignment.com
NASDAQ: ASGN


People First.



From brian <@t> prometheushealthcare.com  Tue Feb 15 09:43:07 2011
From: brian <@t> prometheushealthcare.com (Brian- Prometheus)
Date: Tue Feb 15 09:43:23 2011
Subject: [Histonet] AP Manager Opening in Chicago IL
Message-ID: <007a01cbcd27$0fe3b130$2fab1390$@com>

 

Hospital in Chicago currently searching for an Anatomic Pathology Manager.  

 

Please contact me today for immediate consideration.

 

QUALIFICATIONS: Required: MUST HAVE ANATOMIC PATHOLOGY EXPERIENCE. Master's
degree or bachelor's degree with equivalent experience; prefer 3-5 years
management experience; Demonstrated progressive leadership/management
experience; Demonstrated leadership skills and ability to achieve results;
Demonstrated ability to effectively communicate across multidisciplinary
departments; Problem solving and decision-making skills, ability to
appropriately confront and resolve issues; Ability to build teams, motivate
individuals and groups; Ability to plan, organize and direct the activities
of others.

ESSENTIAL FUNCTIONS: Under the direction of the Director Laboratory, the
Manager oversees the ANATOMIC PATHOLOGY LAB, and has the authority and
accountability for the management of the labs and/or customer service areas
for which he/she has responsibility. This position is key in the planning,
leading and organizing of activities related to quality patient care,
laboratory function and human resource management. This position is
responsible for financial management and has accountability for establishing
and monitoring the operating and capital budgets. This manager is
responsible for translating the hospital's and division's mission,
objectives, policies and procedures into effective action. AA/EOE

 

 

Brian Feldman

Principal

Prometheus Healthcare 

Office 301-693-9057

Fax 301-368-2478

brian  @prometheushealthcare.com

  www.prometheushealthcare.com

*** Stay up to date on the newest positions and healthcare trends nationwide
on Twitter!***

   http://twitter.com/PrometheusBlog

 

From mjdessoye <@t> wvhcs.org  Tue Feb 15 10:49:32 2011
From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J)
Date: Tue Feb 15 10:53:18 2011
Subject: [Histonet] Benchmark Problems
References: 
	
Message-ID: 

Thank you everyone for the helpful replies and info.
 
I followed everyone's suggestions while waiting for service and didn't see any significant improvement.  Once the service tech took everything apart he found the nozzle that controls dispensing of the bulk reagents was partially clogged causing the bulk reagents to be dispensed unevenly (usually totally off the slide!)  Things straightened out afterwards.  
 
Thanks again everyone!
Mike Dessoye

________________________________

From: Mark Tarango [mailto:marktarango@gmail.com]
Sent: Wed 2/9/2011 11:58 AM
To: Dessoye, Michael J
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Benchmark Problems


Hi Mike,
 
Did you wash the bottles and rinse them and then pump the water through the instrument before adding reagents and purging all again?  We had a problem like this about 2 weeks ago.  I think someone loaded the wrong bulk reagent onto the instrument.  Cleaning it, purging with water and adding newly made bulk reagents fixed the problem for us.
 
Mark


On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J  wrote:


	Hello,
	
	We started having a strange problem with our Ventana Benchmark XT.  Out of the blue, our slides stopped staining.  With most antibodies we get no reaction, some that were very strongly positive are now only very lightly staining.  They do appear to counterstain OK.  Following Ventana's recommendations we completely changed out all of our bulk fluids, purged the lines, changed antibodies and DAB kits.  The last shot is some kind of instrument issue that's not triggering an error.  Anyone ever see anything like this?
	
	Mike Dessoye
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From NKonop <@t> chw.org  Tue Feb 15 11:00:55 2011
From: NKonop <@t> chw.org (Konop, Nicole)
Date: Tue Feb 15 11:01:01 2011
Subject: [Histonet] Hema 3/Diff Quik
Message-ID: 

I'm looking for information on what CPT codes are being used for the diff quik/hema 3 stain?  Any feedback is greatly appreciated.  Thanks!

Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department



From NKonop <@t> chw.org  Tue Feb 15 11:27:11 2011
From: NKonop <@t> chw.org (Konop, Nicole)
Date: Tue Feb 15 11:27:17 2011
Subject: [Histonet] RE: Hema 3/Diff Quik
In-Reply-To: 
References: 
Message-ID: 

I should also include that we are using this diff quik as a rapid stain during frozen section in conjunction with a rapid H&E.

Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole
Sent: Tuesday, February 15, 2011 11:01 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Hema 3/Diff Quik

I'm looking for information on what CPT codes are being used for the diff quik/hema 3 stain?  Any feedback is greatly appreciated.  Thanks!

Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Wanda.Smith <@t> HCAhealthcare.com  Tue Feb 15 12:07:09 2011
From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com)
Date: Tue Feb 15 12:07:14 2011
Subject: [Histonet] RE: Hema 3/Diff Quik
In-Reply-To: 
References: 
	
Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EE804DD@NADCWPMSGCMS03.hca.corpad.net>

Good Afternoon,
We use:
88333 Touch prep initial site
88334 Touch prep addition site

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole
Sent: Tuesday, February 15, 2011 12:27 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Hema 3/Diff Quik

I should also include that we are using this diff quik as a rapid stain during frozen section in conjunction with a rapid H&E.

Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole
Sent: Tuesday, February 15, 2011 11:01 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Hema 3/Diff Quik

I'm looking for information on what CPT codes are being used for the diff quik/hema 3 stain?  Any feedback is greatly appreciated.  Thanks!

Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Marcia_Gaiser <@t> ssmhc.com  Tue Feb 15 14:25:18 2011
From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia)
Date: Tue Feb 15 14:25:26 2011
Subject: [Histonet] HT Position in Oklahoma City
Message-ID: <728F817C02110E498D803A7C3B0C6248057D85BD5C@S009-APEXM06.ds.ad.ssmhc.com>

St. Anthony Hospital currently has an excellent opportunity for an experienced Histologic Technician. This position requires Certification as an HT or HLT ? or ? experience acceptable to the Laboratory Director.  Two years of previous histology experience required with IHC and/or grossing experience a plus.  Outstanding benefits package, including generous paid time off.  For consideration, please apply online at www.saintsok.com>>, Ad # 10762, or contact Anna King for additional information at (405) 272-6105.



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From CIngles <@t> uwhealth.org  Tue Feb 15 16:55:24 2011
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Tue Feb 15 16:56:19 2011
Subject: [Histonet] Re: saliva for glycogen hydrolysis
References: 
Message-ID: 

Wouldn't GUM be a more palatable option? Who knows where some of those rubber bands have been!
Claire

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert Richmond
Sent: Mon 2/14/2011 11:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: saliva for glycogen hydrolysis



Here's a truly hi-tech suggestion for doing PAS-diastase stains by the
spit method (which by the way is still how it's done by the small
pathology services I work on):

You can produce copious quantities of saliva by the simple expedient
of chewing on a rubber band for a couple of minutes. This used to be
the technique used to obtain saliva samples for determination of ABH
substance secretor status in the blood bank. (I belong to that elite
20% of donors who are non-secretors, and Lewis-a positive to prove
it.)

Bob Richmond
Samurai pathologist and occasional sialogogue
Knoxville TN

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From mhillmer <@t> dermwisconsin.com  Tue Feb 15 17:02:58 2011
From: mhillmer <@t> dermwisconsin.com (Michael Hillmer)
Date: Tue Feb 15 17:03:02 2011
Subject: [Histonet] Wage question for Wisconsin
Message-ID: <9060F1C2EB3D8A4AAF3B3433C9003230F975A8@hostexchbe1.hosting.tushausweb.com>

We are dermatology clinic in Northeast Wisconsin and we are trying to do
gather accurate wage data.  Can anybody offer wages for an HT, HtL and
lab assistants?  Any help would be greatly appreciated.

 

 

 

 

Thank you-

 

Michael Hillmer PHR

HR Coordinator

Dermatology Associates of Wisconsin

Phone: (920)683-5278

Fax: (920)686-9674

Cell: (920)860-6360

 

The materials and information in this e-mail are confidential and may
contain Protected Health Information covered under the HIPAA Privacy
Rule.  If you are not the intended recipient, be advised that any
unauthorized use, disclosure, copying, distribution, or action taken in
reliance on the contents of this information is strictly forbidden by
law.  If you have received this e-mail in error, please notify me by
reply e-mail and then delete this message.  Do not pass any of this
information to anyone else.  Thank you for your cooperation.

 

From CIngles <@t> uwhealth.org  Tue Feb 15 18:48:09 2011
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Tue Feb 15 18:48:14 2011
Subject: [Histonet] Wage question for Wisconsin
References: <9060F1C2EB3D8A4AAF3B3433C9003230F975A8@hostexchbe1.hosting.tushausweb.com>
Message-ID: 

 
The answer to that question may have to wait a week or two. At least here at the UW. Our wonderful governor is trying to get rid of the state workers unions and collective bargaining. He is trying to push it through (it was just introduced this last Friday), and the vote is slated for later this week. The techs at the UW hospital have fortunately signed their contract that is good until the reopener in 1013 and we are not strictly state employees. However, the way things are looking, it is not out of the question that he may also try to void our contracts.  Our pay MAY go up, but we will no longer have much, if anything, for benefits.  Don't count on sending your kids to the UW colleges if this goes through. 
Sorry, I'm fighting mad right now. I'm not usually big on the unions, but this is HUGE for millions of Wisconsinites. GGRRRRR!
Claire

Were there any other Wisconsin techs at the rally today?  Even the Police and Firefighters unions were down there! 
________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Michael Hillmer
Sent: Tue 2/15/2011 5:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Wage question for Wisconsin



We are dermatology clinic in Northeast Wisconsin and we are trying to do
gather accurate wage data.  Can anybody offer wages for an HT, HtL and
lab assistants?  Any help would be greatly appreciated.


Thank you-



Michael Hillmer PHR

HR Coordinator

Dermatology Associates of Wisconsin

Phone: (920)683-5278




From lpwenk <@t> sbcglobal.net  Tue Feb 15 19:27:32 2011
From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk)
Date: Tue Feb 15 19:27:34 2011
Subject: [Histonet] Wage question for Wisconsin
In-Reply-To: <9060F1C2EB3D8A4AAF3B3433C9003230F975A8@hostexchbe1.hosting.tushausweb.com>
References: <9060F1C2EB3D8A4AAF3B3433C9003230F975A8@hostexchbe1.hosting.tushausweb.com>
Message-ID: <75338FFC879A4718B6404F650270FE3C@HP2010>

The following link is to the ASCP "Laboratory Medicine" article from March 
2009. They do the survey every other year, so there should be another survey 
coming out any month now.
http://www.ascp.org/pdf/Membership-Communications/Wage-and-Vacancy-Survey.aspx

Advance for Medical Laboratory Professionals just published their survey on 
Jan. 31, 2011.
http://laboratorian.advanceweb.com/SharedResources/Downloads/2011/013111/MT013111_SalarySurvey.pdf

Realize that even with a state, there can be several dollars per hour 
difference. Let alone the differences between states in a region. I suggest 
taking the information in the surveys, look at the relationship between 
HT/HTL, MLT/MT, and CT, and then apply the same ratio within your own 
institute.

Hope that helps.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

--------------------------------------------------
From: "Michael Hillmer" 
Sent: Tuesday, February 15, 2011 6:02 PM
To: 
Subject: [Histonet] Wage question for Wisconsin

> We are dermatology clinic in Northeast Wisconsin and we are trying to do
> gather accurate wage data.  Can anybody offer wages for an HT, HtL and
> lab assistants?  Any help would be greatly appreciated.
>
>
>
>
>
>
>
>
>
> Thank you-
>
>
>
> Michael Hillmer PHR
>
> HR Coordinator
>
> Dermatology Associates of Wisconsin
>
> Phone: (920)683-5278
>
> Fax: (920)686-9674
>
> Cell: (920)860-6360
>
>
>
> The materials and information in this e-mail are confidential and may
> contain Protected Health Information covered under the HIPAA Privacy
> Rule.  If you are not the intended recipient, be advised that any
> unauthorized use, disclosure, copying, distribution, or action taken in
> reliance on the contents of this information is strictly forbidden by
> law.  If you have received this e-mail in error, please notify me by
> reply e-mail and then delete this message.  Do not pass any of this
> information to anyone else.  Thank you for your cooperation.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From tjey <@t> hotmail.com  Tue Feb 15 23:09:24 2011
From: tjey <@t> hotmail.com (Tanya Ewing-Finchem)
Date: Tue Feb 15 23:09:29 2011
Subject: [Histonet] Section position on slides
Message-ID: 


I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides.  These are the objectives I am looking to answer.  Is this information found in any publications?
 
1)  Tissue / Section Placement:  Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide?   Perhaps more importantly, where you should NOT place tissue (ie. ?x? mm from the edge of the glass slide)?
 
2)  Diagnosable Slide Staining Area:  With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide?  For instance, would you define that as the area under a traditional coverslip?  Would this be defined as the entire slide below the label?  Or is this some distance from all the edges of the slide?  With some automated systems, it is near impossible to get edge to edge staining.  Is this acceptable?  
 
Thanks for any ideas. 		 	   		  
From amitapandey <@t> torrentpharma.com  Wed Feb 16 00:09:14 2011
From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com)
Date: Wed Feb 16 00:12:20 2011
Subject: [Histonet] Novolink Polymer detection kit
Message-ID: 

Hello Histonetters,

This is first time, I am planning to use Novolink polymer detection kit 
for my mouse monoclonal antibody  (Millipore Anti Na K ATPase and anti 
vimentin) and rabbit polyclonal antibody (Sc-1506R).

 If any body has experience on this polymer kit ,  please share your 
valuable experience which will help me to set up the method in my lab.

Thanks in advance
Amita 
From patpxs <@t> gmail.com  Wed Feb 16 06:02:58 2011
From: patpxs <@t> gmail.com (Paula Sicurello)
Date: Wed Feb 16 06:03:03 2011
Subject: [Histonet] Section position on slides
In-Reply-To: 
References: 
Message-ID: 

Hi Tanya,

It's always best to train people to place the section in the middle of
the slide.  It depends if one has to put multiple sections on a slide.
 Learning how to do proper placement will help them when they have to
cut controls for IHC that are often times placed on the top of the
slide.

Sections placed too close to the very bottom or top sometimes don't
get stained or coverslipped.  Too close to the edges and you can
suffer wrap around or overlap, causing the section to be difficult for
the pathologist to read.  Boy howdy!  They will let you know if they
don't like the placement.

Therefore, train for centering the section as best as possible.

I hope this helps,

Paula  :-)

On Wed, Feb 16, 2011 at 12:09 AM, Tanya Ewing-Finchem  wrote:
>
> I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. ?These are the objectives I am looking to answer. ?Is this information found in any publications?
>
> 1) ?Tissue / Section Placement: ?Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? ? Perhaps more importantly, where you should NOT place tissue (ie. ?x? mm from the edge of the glass slide)?
>
> 2) ?Diagnosable Slide Staining Area: ?With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? ?For instance, would you define that as the area under a traditional coverslip? ?Would this be defined as the entire slide below the label? ?Or is this some distance from all the edges of the slide? ?With some automated systems, it is near impossible to get edge to edge staining. ?Is this acceptable?
>
> Thanks for any ideas. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Electron Microscope Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P: ?919.684.2091

From amitapandey <@t> torrentpharma.com  Wed Feb 16 06:12:37 2011
From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com)
Date: Wed Feb 16 06:15:38 2011
Subject: [Histonet] Na K ATpase immunohistochemistry on rat FFPE tissue
Message-ID: 

Does any body have experienced on Anti Na K ATpase immunohistochemistry on 
FFPE rat kidney tissue?
 I am finding difficult to have any supportive lit regarding this, most of 
them support to frozen sectioning. 

Thanks a lot

Amita
From sgoebel <@t> mirnarx.com  Wed Feb 16 08:56:20 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Wed Feb 16 08:56:25 2011
Subject: [Histonet] Wage question for Wisconsin
In-Reply-To: 
References: <9060F1C2EB3D8A4AAF3B3433C9003230F975A8@hostexchbe1.hosting.tushausweb.com>
	
Message-ID: 

At least you have a union!!

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles
Claire 
Sent: Tuesday, February 15, 2011 6:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Wage question for Wisconsin

 
The answer to that question may have to wait a week or two. At least
here at the UW. Our wonderful governor is trying to get rid of the state
workers unions and collective bargaining. He is trying to push it
through (it was just introduced this last Friday), and the vote is
slated for later this week. The techs at the UW hospital have
fortunately signed their contract that is good until the reopener in
1013 and we are not strictly state employees. However, the way things
are looking, it is not out of the question that he may also try to void
our contracts.  Our pay MAY go up, but we will no longer have much, if
anything, for benefits.  Don't count on sending your kids to the UW
colleges if this goes through. 
Sorry, I'm fighting mad right now. I'm not usually big on the unions,
but this is HUGE for millions of Wisconsinites. GGRRRRR!
Claire

Were there any other Wisconsin techs at the rally today?  Even the
Police and Firefighters unions were down there! 
________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Michael
Hillmer
Sent: Tue 2/15/2011 5:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Wage question for Wisconsin



We are dermatology clinic in Northeast Wisconsin and we are trying to do
gather accurate wage data.  Can anybody offer wages for an HT, HtL and
lab assistants?  Any help would be greatly appreciated.


Thank you-



Michael Hillmer PHR

HR Coordinator

Dermatology Associates of Wisconsin

Phone: (920)683-5278




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From sgoebel <@t> mirnarx.com  Wed Feb 16 08:59:44 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Wed Feb 16 08:59:47 2011
Subject: [Histonet] Section position on slides
In-Reply-To: 
References: 
Message-ID: 

I learned from an old school HT and studied using the Carson book.  In
this book there are several pages on where to put sections.  If you
don't have the Frieda Carson book (or "the bible" as I refer to it), get
it.  A new edition just came out about a year ago.  If you're an ASCP
member I think the price is reduced...
For the most part, just put everything as close to the middle of the
clear glass part of the slide as possible.  This also does well for IHC
automation.  I think most automated machines you can adjust the drop
range too if you want to put the sections on the bottom or top?
Good Luck!

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya
Ewing-Finchem
Sent: Tuesday, February 15, 2011 11:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Section position on slides


I am trying to put together a training document around microtomy and
sectioning and am finding it hard to find information around the
placement of the actual sections on the slides.  These are the
objectives I am looking to answer.  Is this information found in any
publications?
 
1)  Tissue / Section Placement:  Are there published guidelines /
documentation on precisely where you should place tissue sections on a
25mm x 75mm glass slide?   Perhaps more importantly, where you should
NOT place tissue (ie. "x" mm from the edge of the glass slide)?
 
2)  Diagnosable Slide Staining Area:  With automation becoming more
widely used in IHC, are there published guidelines / documentation on
the usable or diagnosable staining area on a 25mm x 75mm glass slide?
For instance, would you define that as the area under a traditional
coverslip?  Would this be defined as the entire slide below the label?
Or is this some distance from all the edges of the slide?  With some
automated systems, it is near impossible to get edge to edge staining.
Is this acceptable?  

 
Thanks for any ideas.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From cgill <@t> marylandgeneral.org  Wed Feb 16 09:15:29 2011
From: cgill <@t> marylandgeneral.org (Gill, Caula A.)
Date: Wed Feb 16 09:15:34 2011
Subject: [Histonet] Re: saliva for glycogen hydrolysis
In-Reply-To: 
References: 
	
Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9F38@MDGEN-EXCH1.marylandgeneral.org>

I agree, gum sounds better not to mention the taste
CG 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles
Claire 
Sent: Tuesday, February 15, 2011 5:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis

Wouldn't GUM be a more palatable option? Who knows where some of those
rubber bands have been!
Claire

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert
Richmond
Sent: Mon 2/14/2011 11:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: saliva for glycogen hydrolysis



Here's a truly hi-tech suggestion for doing PAS-diastase stains by the
spit method (which by the way is still how it's done by the small
pathology services I work on):

You can produce copious quantities of saliva by the simple expedient of
chewing on a rubber band for a couple of minutes. This used to be the
technique used to obtain saliva samples for determination of ABH
substance secretor status in the blood bank. (I belong to that elite 20%
of donors who are non-secretors, and Lewis-a positive to prove
it.)

Bob Richmond
Samurai pathologist and occasional sialogogue Knoxville TN

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From bakevictoria <@t> gmail.com  Wed Feb 16 09:25:27 2011
From: bakevictoria <@t> gmail.com (Victoria Baker)
Date: Wed Feb 16 09:25:55 2011
Subject: [Histonet] Wage question for Wisconsin
In-Reply-To: <9060F1C2EB3D8A4AAF3B3433C9003230F975A8@hostexchbe1.hosting.tushausweb.com>
References: <9060F1C2EB3D8A4AAF3B3433C9003230F975A8@hostexchbe1.hosting.tushausweb.com>
Message-ID: 

Michael,

ASCP has a wage/salary survey that they distribute based upon region and
certification.  Some have said that this is biased as not everyone does
participate.  I tend to agree with the survey, but also recognize that
dermatopathology is a 'specialty' type lab.  I worked in Dermpath at NYU
back in the 80's to early 90's and our salaries were not identical to the
clinical labs as we were a part of the medical school and not the hospital.
Yes the hospital was 1199 at that time, I cannot say if they are still.

My recommendation is to go with what you can find from the survey, but also
network with other dermpath or other specialty labs in your area that you
may know or who you can connect with through others - either on the
listserver or off - to possibly get a better sounding of salaries.  HR at
your hospital might be also able to help if you have someone there that you
can work with and trust to obtain some of the data you're looking for and
understands what your specific needs are within the lab.  I would include
your pathologists in this as much as possible as they will be needed to
support your request(s) as they are essentially your clients.

Vikki

On Tue, Feb 15, 2011 at 6:02 PM, Michael Hillmer  wrote:

> We are dermatology clinic in Northeast Wisconsin and we are trying to do
> gather accurate wage data.  Can anybody offer wages for an HT, HtL and
> lab assistants?  Any help would be greatly appreciated.
>
>
>
>
>
>
>
>
>
> Thank you-
>
>
>
> Michael Hillmer PHR
>
> HR Coordinator
>
> Dermatology Associates of Wisconsin
>
> Phone: (920)683-5278
>
> Fax: (920)686-9674
>
> Cell: (920)860-6360
>
>
>
> The materials and information in this e-mail are confidential and may
> contain Protected Health Information covered under the HIPAA Privacy
> Rule.  If you are not the intended recipient, be advised that any
> unauthorized use, disclosure, copying, distribution, or action taken in
> reliance on the contents of this information is strictly forbidden by
> law.  If you have received this e-mail in error, please notify me by
> reply e-mail and then delete this message.  Do not pass any of this
> information to anyone else.  Thank you for your cooperation.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From POWELL_SA <@t> mercer.edu  Wed Feb 16 09:27:55 2011
From: POWELL_SA <@t> mercer.edu (Shirley A. Powell)
Date: Wed Feb 16 09:28:01 2011
Subject: [Histonet] Correction, Correction  on cutoff date for GSH meeting
Message-ID: <9BF995BC0E47744E9673A41486E24EE22DBB3A7DE3@MERCERMAIL.MercerU.local>

Correction: There is a correction on the cutoff date for the hotel reservations.  Cutoff date for discounted room rate is February 23rd, Not March 1st.  There are several other events going on that weekend and the hotel is filling up fast.  Please make your reservations and register soon so you will not miss out on the great room rate and a great meeting.

Shirley Powell


Hi Georgia, Alabama, ALL histotechs,



The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line.  The invitation extends to any other states as well.  Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge.



The deadline for making hotel reservations is March 1, 2011  so that gives you a month to make your plans to attend, don't delay.  The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292.  Room rates start at $99 which includes Continental Breakfast and Admission to the Park.  For more information about things to do at Callaway click on the link here:   http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx



Our theme this year is "METAMORPHOSIS:  Transforming Histotechs."  The complete program can be downloaded from our website at this link:  www.histosearch.com/gsh> then click on GSH symposium link at the bottom of the home page.  There you will find the complete program with registration form.  The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting.  If anyone has questions, please contact me for assistance.



Come TRANSFORM yourselves.





Shirley Powell

GSH Secretary


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

From sbreeden <@t> nmda.nmsu.edu  Wed Feb 16 09:59:36 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Wed Feb 16 09:59:41 2011
Subject: [Histonet] Pathco double-blade handle
Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4766A@nmdamailsvr.nmda.ad.nmsu.edu>

Am looking for this item to replace one that was older than I am.  I
need the handle (perhaps Pathco #D2877) for the blades (Pathco
#D2878-2C).  The catch is that I want the SOLID BRASS handle - not the
aluminum.  I've "googled" all over the place - can anyone help me find
these handles?  Muchas gracias!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From jstaruk <@t> masshistology.com  Wed Feb 16 10:09:31 2011
From: jstaruk <@t> masshistology.com (jstaruk)
Date: Wed Feb 16 10:09:30 2011
Subject: [Histonet] Pathco double-blade handle
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4766A@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B02E4766A@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <005501cbcdf3$e623bd40$b26b37c0$@masshistology.com>

I've found two brass handles on Ebay in the last few years.  I don't know if
you're aware of this but the exact same blades that fit these handles can be
purchased at Home Depot in the carpet section.  $10 for 50 of them.

 

Jim

 

_______________________

James E. Staruk HT(ASCP)

   www.masshistology.com

   www.nehorselabs.com

 

 

From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Wednesday, February 16, 2011 11:00 AM
To: Histonet
Subject: [Histonet] Pathco double-blade handle

 

Am looking for this item to replace one that was older than I am.  I
need the handle (perhaps Pathco #D2877) for the blades (Pathco
#D2878-2C).  The catch is that I want the SOLID BRASS handle - not the
aluminum.  I've "googled" all over the place - can anyone help me find
these handles?  Muchas gracias!



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From caymanfleck <@t> gmail.com  Wed Feb 16 10:12:00 2011
From: caymanfleck <@t> gmail.com (caymanfleck@gmail.com)
Date: Wed Feb 16 10:12:04 2011
Subject: [Histonet] Exam Prep
Message-ID: 

Hello Histoland,

I'm preparing for the HTL exam after being trained on-the-job and working in
histo for a few years.  I'm using the standard 'Theory and practice...' text
as my main resource.  I'm wondering if anyone has used either the ASCP
practice tests (a set of 5 are available from ascp for about $30) or the NSH
Self-assessment Program (which has modules that correspond to the exam
sections).  The NSH program is expensive at about $240 but I don't know if
it's worth it.  Has anyone used either of these resources?

Thanks!
From Nacaela.Johnson <@t> USONCOLOGY.COM  Wed Feb 16 10:23:52 2011
From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela)
Date: Wed Feb 16 10:24:56 2011
Subject: [Histonet] Exam Prep
In-Reply-To: 
References: 
Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D539@txhous1eb012.uson.usoncology.int>

I just used the Freida Carson Histotechnology A Self-Instructional Test.



Thanks,
 
Nacaela Johnson, HTL (ASCP)
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson@USOncology.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
caymanfleck@gmail.com
Sent: Wednesday, February 16, 2011 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Exam Prep

Hello Histoland,

I'm preparing for the HTL exam after being trained on-the-job and
working in histo for a few years.  I'm using the standard 'Theory and
practice...' text as my main resource.  I'm wondering if anyone has used
either the ASCP practice tests (a set of 5 are available from ascp for
about $30) or the NSH Self-assessment Program (which has modules that
correspond to the exam sections).  The NSH program is expensive at about
$240 but I don't know if it's worth it.  Has anyone used either of these
resources?

Thanks!
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From sgoebel <@t> mirnarx.com Wed Feb 16 10:35:21 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Feb 16 10:35:24 2011 Subject: [Histonet] Exam Prep In-Reply-To: <6DBD71C31D7E444482E5D3DFBC202D260245D539@txhous1eb012.uson.usoncology.int> References: <6DBD71C31D7E444482E5D3DFBC202D260245D539@txhous1eb012.uson.usoncology.int> Message-ID: If you basically memorize this book, and then have a pretty good understanding of IHC...then you should do fine. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela Sent: Wednesday, February 16, 2011 10:24 AM To: caymanfleck@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Exam Prep I just used the Freida Carson Histotechnology A Self-Instructional Test. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caymanfleck@gmail.com Sent: Wednesday, February 16, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exam Prep Hello Histoland, I'm preparing for the HTL exam after being trained on-the-job and working in histo for a few years. I'm using the standard 'Theory and practice...' text as my main resource. I'm wondering if anyone has used either the ASCP practice tests (a set of 5 are available from ascp for about $30) or the NSH Self-assessment Program (which has modules that correspond to the exam sections). The NSH program is expensive at about $240 but I don't know if it's worth it. Has anyone used either of these resources? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Wed Feb 16 11:08:07 2011 From: bill501 <@t> mindspring.com (Bill B.) Date: Wed Feb 16 11:08:12 2011 Subject: [Histonet] Section position on slides In-Reply-To: References: Message-ID: As a pathologist I have some preferences on how certain tissues are oriented on a slide and work with my histotechs so they can learn my prefs. I also like my slides in certain orientation in the slide tray. EG, for orientation of skin, I like the the dermis at the top when I am looking at the slide. Some other tissues can be so oriented, some cannot. We often put multiple levels on one slide and I would prefer them to be all oriented similarly and in a straight line. The most annoying thing I see beyond coverslipping the wrong side, is the label covering a part of the specimen. I don't care how close to the edge a section is as long as it is all on the slide and covered by the cover-slip or mounting medium. I have no idea is these preferences are weird. My point is the answer to you 1st question may depend on your particular pathologists' preferences. BIll Blank At 10:09 PM -0700 2/15/11, Tanya Ewing-Finchem wrote: >1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. ?x? mm from the edge of the glass slide)? From jqb7 <@t> cdc.gov Wed Feb 16 11:20:57 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Feb 16 11:21:35 2011 Subject: [Histonet] Section position on slides In-Reply-To: References: Message-ID: I always try to center the section equally from sides and top-bottom as possible. This means measure from the bottom of the frosted edge as the "top". The Artisan special stains system has a clip that attaches around the slide to allow reagents to pool onto the sections and incubate. If the section is too close to the sides then these areas do not stain adequately. Sometimes if the tissue section itself is very large this is unavoidable. With automated coverslippers you must also consider placement of tissue to allow for proper coverage. If I am cutting multiple unstained slides for subsequent testing I try to orient the tissue the same on each slide to facilitate the reading of these slides by the pathologist's Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Wednesday, February 16, 2011 12:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. "x" mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Wed Feb 16 11:27:32 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Feb 16 11:27:37 2011 Subject: [Histonet] Section position on slides In-Reply-To: References: Message-ID: Since automation is becoming more and more a part of all Histology labs the demands of placement of the tissue on the slides varies for different instruments. Stainers, coverslippers and now with slide scanning as well. So I do not believe that there is a silver bullet answer. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 16, 2011 12:21 PM To: Tanya Ewing-Finchem; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Section position on slides I always try to center the section equally from sides and top-bottom as possible. This means measure from the bottom of the frosted edge as the "top". The Artisan special stains system has a clip that attaches around the slide to allow reagents to pool onto the sections and incubate. If the section is too close to the sides then these areas do not stain adequately. Sometimes if the tissue section itself is very large this is unavoidable. With automated coverslippers you must also consider placement of tissue to allow for proper coverage. If I am cutting multiple unstained slides for subsequent testing I try to orient the tissue the same on each slide to facilitate the reading of these slides by the pathologist's Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Wednesday, February 16, 2011 12:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. "x" mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Wed Feb 16 11:28:33 2011 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Feb 16 11:28:48 2011 Subject: [Histonet] Apologize for Spam Message-ID: <8CD9C1611DA5486-1F74-2DDE@Webmail-d108.sysops.aol.com> Someone has hacked into my email and it is sending nonsense emails to everyone in my address book..... I have changed my password to prevent this from happening again. Again, I am truly sorry. Roxanne From bakevictoria <@t> gmail.com Wed Feb 16 12:23:02 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Feb 16 12:23:08 2011 Subject: [Histonet] Section position on slides In-Reply-To: References: Message-ID: Automated coverslipping instruments are defined by the manufacturer, but modifications on some systems can be done. Most clinical labs who utilize automation use 24X50mm - as this coverslip will cover most of the slide. Tissue placement is pretty much determined by laboratory needs. Sheehan & Hrapchak had a short segment about tissue placement, but it was in regards to the tissue orientation for microscopic assessment requirements. There is nothing in granite about tissue placement that I know of. Some tools that I worked with were using the Cytospin large rectangular slides to show residents/students/collegues where the tissue placement needed to be. Once they were able to get the tissue section in there we moved on. While to most of us who work in Histo sort of take putting sections on a slide for granted (yes we did work hard to get there) getting the mechanics of it takes patience, skill and practice. Keep it simple and build up from there. Vikki On Wed, Feb 16, 2011 at 12:27 PM, Helen Fedor wrote: > Since automation is becoming more and more a part of all Histology labs the > demands of placement of the tissue on the slides varies for different > instruments. Stainers, coverslippers and now with slide scanning as well. So > I do not believe that there is a silver bullet answer. > > Helen L. Fedor > > Tissue Microarray Lab, Manager > Prostate Spore Lab, Manager > Johns Hopkins University > 600 N. Wolfe St, | Marburg Room 406 > Baltimore, MD | 21287-7065 > > 410.614.1660 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine > (CDC/OID/NCEZID) > Sent: Wednesday, February 16, 2011 12:21 PM > To: Tanya Ewing-Finchem; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Section position on slides > > I always try to center the section equally from sides and top-bottom as > possible. This means measure from the bottom of the frosted edge as the > "top". The Artisan special stains system has a clip that attaches around > the slide to allow reagents to pool onto the sections and incubate. If the > section is too close to the sides then these areas do not stain adequately. > Sometimes if the tissue section itself is very large this is unavoidable. > With automated coverslippers you must also consider placement of tissue to > allow for proper coverage. > > If I am cutting multiple unstained slides for subsequent testing I try to > orient the tissue the same on each slide to facilitate the reading of these > slides by the pathologist's > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya > Ewing-Finchem > Sent: Wednesday, February 16, 2011 12:09 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Section position on slides > > > I am trying to put together a training document around microtomy and > sectioning and am finding it hard to find information around the placement > of the actual sections on the slides. These are the objectives I am looking > to answer. Is this information found in any publications? > > 1) Tissue / Section Placement: Are there published guidelines / > documentation on precisely where you should place tissue sections on a 25mm > x 75mm glass slide? Perhaps more importantly, where you should NOT place > tissue (ie. "x" mm from the edge of the glass slide)? > > 2) Diagnosable Slide Staining Area: With automation becoming more widely > used in IHC, are there published guidelines / documentation on the usable or > diagnosable staining area on a 25mm x 75mm glass slide? For instance, would > you define that as the area under a traditional coverslip? Would this be > defined as the entire slide below the label? Or is this some distance from > all the edges of the slide? With some automated systems, it is near > impossible to get edge to edge staining. Is this acceptable? > > > > Thanks for any ideas. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JEllin <@t> yumaregional.org Wed Feb 16 12:38:04 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Feb 16 12:38:11 2011 Subject: [Histonet] Information Please Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D68ED@EXCHANGECLUSTER.yumaregional.local> Wanting to get information on anyone that has already implemented Epic and offered the Epic ambulatory piece to there clients. Are you also offering this technology with interface to competitive laboratories like, Quest, Lab Corp, etc? ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From DKBoyd <@t> chs.net Wed Feb 16 12:39:22 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Feb 16 12:39:31 2011 Subject: [Histonet] Ventana Rep. Message-ID: Can a Ventana Representative for the Southern Virginia region, please give me a call at the number below. Please only a Ventana rep. I do not have authorization to deal with another company at this time. Thank you. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rochan <@t> premierlab.com Wed Feb 16 12:47:51 2011 From: rochan <@t> premierlab.com (Rochan Moir-Dial) Date: Wed Feb 16 12:45:26 2011 Subject: [Histonet] Exam Prep In-Reply-To: <6DBD71C31D7E444482E5D3DFBC202D260245D539@txhous1eb012.uson.usoncology.int> References: <6DBD71C31D7E444482E5D3DFBC202D260245D539@txhous1eb012.uson.usoncology.int> Message-ID: I also used Freida Carson, Histotechnology A Self-Instructional Test, 3rd Edition. As well as, the Freida Carson, Companion Study Flash Cards that goes along with the 3rd Edition text book. Study the objectives and your golden. Rochan Moir-Dial, HTL(ASCP) Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 Phone: (303) 682-3949 Fax (303) 682-9060 rochan@premierlab.com www.premierlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela Sent: Wednesday, February 16, 2011 9:24 AM To: caymanfleck@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Exam Prep I just used the Freida Carson Histotechnology A Self-Instructional Test. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caymanfleck@gmail.com Sent: Wednesday, February 16, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exam Prep Hello Histoland, I'm preparing for the HTL exam after being trained on-the-job and working in histo for a few years. I'm using the standard 'Theory and practice...' text as my main resource. I'm wondering if anyone has used either the ASCP practice tests (a set of 5 are available from ascp for about $30) or the NSH Self-assessment Program (which has modules that correspond to the exam sections). The NSH program is expensive at about $240 but I don't know if it's worth it. Has anyone used either of these resources? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Wed Feb 16 13:16:30 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Feb 16 13:16:48 2011 Subject: [Histonet] Colorado Sate Histo Meeting, April 29-30, 2011 Message-ID: <4D5BC01E020000A800056647@ns.luhcares.org> Hi, All are welcome. It is a great program this year. Attached is the program for the 2011 CSH meeting, which will be held April 29th & 30th at the Embassy Suites hotel in Loveland, CO. Online registration and credit card payment is available on the CSH website at http://www.coloradohisto.org/2011/meeting.htm Ciao Matt Lunetta HT (ASCP) From mpowers <@t> dpspa.com Wed Feb 16 13:18:23 2011 From: mpowers <@t> dpspa.com (Marian Powers) Date: Wed Feb 16 13:18:30 2011 Subject: [Histonet] NY lab permit Message-ID: Hello all, Anyone out there have experience in applying for a NY state lab permit? How long did it take? How difficult was it? Could anyone recommend a lab consultant on this task? Thanks in advance, -- *Marian L. Powers, BSOM, HT(ASCP) * * * * * * * From amylee779 <@t> yahoo.com Wed Feb 16 14:06:41 2011 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Wed Feb 16 14:06:44 2011 Subject: [Histonet] neutrophil antibody on rat FFP section Message-ID: <544851.36245.qm@web38005.mail.mud.yahoo.com> Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. ? Could anybody recommend a good antibody? ? Thanks in advance, ? Amy ? From traczyk7 <@t> aol.com Wed Feb 16 15:31:36 2011 From: traczyk7 <@t> aol.com (traczyk7@aol.com) Date: Wed Feb 16 15:31:45 2011 Subject: [Histonet] CellSoft vs. Sodium Hydroxide Message-ID: <8CD9C38063E34B6-12F8-6B35@webmail-d064.sysops.aol.com> Greetings one & all: Has anyone tried "CellSoft" from CellPath in the UK? I received a sample today and after reviewing the MSDS that came with it, I'm skeptical about the claim in their literature that it "reduces the hazard risk for the user". I've got 10% sodium hydroxide on hand for softening nails so if anyone has already done a product comparison, I'd love to hear from you. Thanks. Dorothy From Eric.Hoy <@t> UTSouthwestern.edu Wed Feb 16 15:33:23 2011 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Wed Feb 16 15:33:29 2011 Subject: [Histonet] Re: saliva for glycogen hydrolysis In-Reply-To: <485955ee55d94d6aaafbc24fbb067857@SWMSHUB3.swmed.org> Message-ID: > From: "Ingles Claire " > Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis > Wouldn't GUM be a more palatable option? Who knows where some of those rubber > bands have been! > Claire > Back in my early days in the lab, when I was in the US Navy (wooden ships and Folin-Wu glucose), I was involved in a project that required us to collect fairly large (10-15 mL) saliva samples from Navy recruits who were part of a research study. We used a wax gum, which I believe was manufactured by Wrigley's gum company. The sticks were the same size and shape as gum (back then gum looked like a microscope slide, not all the shapes that are currently available) and they were individually wrapped like gum. They tasted like paraffin, but they induced copious salivation, and served our purpose well. I don't know if this product is still available. Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== From amitapandey <@t> torrentpharma.com Wed Feb 16 22:13:59 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Wed Feb 16 22:17:02 2011 Subject: [Histonet] Re: Novolink polymer kit In-Reply-To: <001601cbce05$40639ee0$c12adca0$@maxvisionbio.com> References: <001601cbce05$40639ee0$c12adca0$@maxvisionbio.com> Message-ID: Thanks Michelle and all histonetters for your suggestions. After your feed back, i am finalizing the Novolink Polymer-HRP detection kit for my work. I am going to use this kit for single labelling of the antibody. i will let u know about my result as it comes. Amita From mminhui <@t> emory.edu Thu Feb 17 08:33:25 2011 From: mminhui <@t> emory.edu (Ma, Minhui) Date: Thu Feb 17 08:33:35 2011 Subject: [Histonet] RE: Histonet Digest, Vol 87, Issue 28 In-Reply-To: <2615178028147681085057692487831@psmtp.com> References: <2615178028147681085057692487831@psmtp.com> Message-ID: <43DEC30DB656564EB330190305519FB701ADFE534CAF@EXCHANGE22.Enterprise.emory.net> Dear everyone, I'm wondering where I can order the Picro-Mallory Trichrome Stain kit? I couldn't find it online excepted some Italian companies. Thanks, Minhui Ma MD,HTL Emory University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, February 16, 2011 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Hema 3/Diff Quik (Wanda.Smith@HCAhealthcare.com) 2. HT Position in Oklahoma City (Gaiser, Marcia) 3. RE: Re: saliva for glycogen hydrolysis (Ingles Claire ) 4. Wage question for Wisconsin (Michael Hillmer) 5. RE: Wage question for Wisconsin (Ingles Claire ) 6. Re: Wage question for Wisconsin (Lee & Peggy Wenk) 7. Section position on slides (Tanya Ewing-Finchem) 8. Novolink Polymer detection kit (amitapandey@torrentpharma.com) 9. Re: Section position on slides (Paula Sicurello) 10. Na K ATpase immunohistochemistry on rat FFPE tissue (amitapandey@torrentpharma.com) 11. RE: Wage question for Wisconsin (sgoebel@mirnarx.com) 12. RE: Section position on slides (sgoebel@mirnarx.com) 13. RE: Re: saliva for glycogen hydrolysis (Gill, Caula A.) 14. Re: Wage question for Wisconsin (Victoria Baker) 15. Correction, Correction on cutoff date for GSH meeting (Shirley A. Powell) 16. Pathco double-blade handle (Breeden, Sara) 17. RE: Pathco double-blade handle (jstaruk) 18. Exam Prep (caymanfleck@gmail.com) 19. RE: Exam Prep (Johnson, Nacaela) 20. RE: Exam Prep (sgoebel@mirnarx.com) 21. Re: Section position on slides (Bill B.) 22. RE: Section position on slides (Bartlett, Jeanine (CDC/OID/NCEZID)) 23. RE: Section position on slides (Helen Fedor) ---------------------------------------------------------------------- Message: 1 Date: Tue, 15 Feb 2011 12:07:09 -0600 From: Subject: [Histonet] RE: Hema 3/Diff Quik To: , Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139EE804DD@NADCWPMSGCMS03.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Good Afternoon, We use: 88333 Touch prep initial site 88334 Touch prep addition site WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, February 15, 2011 12:27 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Hema 3/Diff Quik I should also include that we are using this diff quik as a rapid stain during frozen section in conjunction with a rapid H&E. Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, February 15, 2011 11:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Hema 3/Diff Quik I'm looking for information on what CPT codes are being used for the diff quik/hema 3 stain? Any feedback is greatly appreciated. Thanks! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 15 Feb 2011 14:25:18 -0600 From: "Gaiser, Marcia" Subject: [Histonet] HT Position in Oklahoma City To: "histonet@lists.utsouthwestern.edu" Message-ID: <728F817C02110E498D803A7C3B0C6248057D85BD5C@S009-APEXM06.ds.ad.ssmhc.com> Content-Type: text/plain; charset="Windows-1252" St. Anthony Hospital currently has an excellent opportunity for an experienced Histologic Technician. This position requires Certification as an HT or HLT ? or ? experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.com>>, Ad # 10762, or contact Anna King for additional information at (405) 272-6105. Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ------------------------------ Message: 3 Date: Tue, 15 Feb 2011 16:55:24 -0600 From: "Ingles Claire " Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Wouldn't GUM be a more palatable option? Who knows where some of those rubber bands have been! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Mon 2/14/2011 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: saliva for glycogen hydrolysis Here's a truly hi-tech suggestion for doing PAS-diastase stains by the spit method (which by the way is still how it's done by the small pathology services I work on): You can produce copious quantities of saliva by the simple expedient of chewing on a rubber band for a couple of minutes. This used to be the technique used to obtain saliva samples for determination of ABH substance secretor status in the blood bank. (I belong to that elite 20% of donors who are non-secretors, and Lewis-a positive to prove it.) Bob Richmond Samurai pathologist and occasional sialogogue Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 15 Feb 2011 17:02:58 -0600 From: "Michael Hillmer" Subject: [Histonet] Wage question for Wisconsin To: Message-ID: <9060F1C2EB3D8A4AAF3B3433C9003230F975A8@hostexchbe1.hosting.tushausweb.com> Content-Type: text/plain; charset="us-ascii" We are dermatology clinic in Northeast Wisconsin and we are trying to do gather accurate wage data. Can anybody offer wages for an HT, HtL and lab assistants? Any help would be greatly appreciated. Thank you- Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 Fax: (920)686-9674 Cell: (920)860-6360 The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ------------------------------ Message: 5 Date: Tue, 15 Feb 2011 18:48:09 -0600 From: "Ingles Claire " Subject: RE: [Histonet] Wage question for Wisconsin To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" The answer to that question may have to wait a week or two. At least here at the UW. Our wonderful governor is trying to get rid of the state workers unions and collective bargaining. He is trying to push it through (it was just introduced this last Friday), and the vote is slated for later this week. The techs at the UW hospital have fortunately signed their contract that is good until the reopener in 1013 and we are not strictly state employees. However, the way things are looking, it is not out of the question that he may also try to void our contracts. Our pay MAY go up, but we will no longer have much, if anything, for benefits. Don't count on sending your kids to the UW colleges if this goes through. Sorry, I'm fighting mad right now. I'm not usually big on the unions, but this is HUGE for millions of Wisconsinites. GGRRRRR! Claire Were there any other Wisconsin techs at the rally today? Even the Police and Firefighters unions were down there! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Michael Hillmer Sent: Tue 2/15/2011 5:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wage question for Wisconsin We are dermatology clinic in Northeast Wisconsin and we are trying to do gather accurate wage data. Can anybody offer wages for an HT, HtL and lab assistants? Any help would be greatly appreciated. Thank you- Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 ------------------------------ Message: 6 Date: Tue, 15 Feb 2011 20:27:32 -0500 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] Wage question for Wisconsin To: "Michael Hillmer" , Message-ID: <75338FFC879A4718B6404F650270FE3C@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original The following link is to the ASCP "Laboratory Medicine" article from March 2009. They do the survey every other year, so there should be another survey coming out any month now. http://www.ascp.org/pdf/Membership-Communications/Wage-and-Vacancy-Survey.aspx Advance for Medical Laboratory Professionals just published their survey on Jan. 31, 2011. http://laboratorian.advanceweb.com/SharedResources/Downloads/2011/013111/MT013111_SalarySurvey.pdf Realize that even with a state, there can be several dollars per hour difference. Let alone the differences between states in a region. I suggest taking the information in the surveys, look at the relationship between HT/HTL, MLT/MT, and CT, and then apply the same ratio within your own institute. Hope that helps. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Michael Hillmer" Sent: Tuesday, February 15, 2011 6:02 PM To: Subject: [Histonet] Wage question for Wisconsin > We are dermatology clinic in Northeast Wisconsin and we are trying to do > gather accurate wage data. Can anybody offer wages for an HT, HtL and > lab assistants? Any help would be greatly appreciated. > > > > > > > > > > Thank you- > > > > Michael Hillmer PHR > > HR Coordinator > > Dermatology Associates of Wisconsin > > Phone: (920)683-5278 > > Fax: (920)686-9674 > > Cell: (920)860-6360 > > > > The materials and information in this e-mail are confidential and may > contain Protected Health Information covered under the HIPAA Privacy > Rule. If you are not the intended recipient, be advised that any > unauthorized use, disclosure, copying, distribution, or action taken in > reliance on the contents of this information is strictly forbidden by > law. If you have received this e-mail in error, please notify me by > reply e-mail and then delete this message. Do not pass any of this > information to anyone else. Thank you for your cooperation. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 15 Feb 2011 22:09:24 -0700 From: Tanya Ewing-Finchem Subject: [Histonet] Section position on slides To: Message-ID: Content-Type: text/plain; charset="Windows-1252" I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. ?x? mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. ------------------------------ Message: 8 Date: Wed, 16 Feb 2011 11:39:14 +0530 From: amitapandey@torrentpharma.com Subject: [Histonet] Novolink Polymer detection kit To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello Histonetters, This is first time, I am planning to use Novolink polymer detection kit for my mouse monoclonal antibody (Millipore Anti Na K ATPase and anti vimentin) and rabbit polyclonal antibody (Sc-1506R). If any body has experience on this polymer kit , please share your valuable experience which will help me to set up the method in my lab. Thanks in advance Amita ------------------------------ Message: 9 Date: Wed, 16 Feb 2011 07:02:58 -0500 From: Paula Sicurello Subject: Re: [Histonet] Section position on slides To: Tanya Ewing-Finchem Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Hi Tanya, It's always best to train people to place the section in the middle of the slide. It depends if one has to put multiple sections on a slide. Learning how to do proper placement will help them when they have to cut controls for IHC that are often times placed on the top of the slide. Sections placed too close to the very bottom or top sometimes don't get stained or coverslipped. Too close to the edges and you can suffer wrap around or overlap, causing the section to be difficult for the pathologist to read. Boy howdy! They will let you know if they don't like the placement. Therefore, train for centering the section as best as possible. I hope this helps, Paula :-) On Wed, Feb 16, 2011 at 12:09 AM, Tanya Ewing-Finchem wrote: > > I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. ?These are the objectives I am looking to answer. ?Is this information found in any publications? > > 1) ?Tissue / Section Placement: ?Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? ? Perhaps more importantly, where you should NOT place tissue (ie. ?x? mm from the edge of the glass slide)? > > 2) ?Diagnosable Slide Staining Area: ?With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? ?For instance, would you define that as the area under a traditional coverslip? ?Would this be defined as the entire slide below the label? ?Or is this some distance from all the edges of the slide? ?With some automated systems, it is near impossible to get edge to edge staining. ?Is this acceptable? > > Thanks for any ideas. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: ?919.684.2091 ------------------------------ Message: 10 Date: Wed, 16 Feb 2011 17:42:37 +0530 From: amitapandey@torrentpharma.com Subject: [Histonet] Na K ATpase immunohistochemistry on rat FFPE tissue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Does any body have experienced on Anti Na K ATpase immunohistochemistry on FFPE rat kidney tissue? I am finding difficult to have any supportive lit regarding this, most of them support to frozen sectioning. Thanks a lot Amita ------------------------------ Message: 11 Date: Wed, 16 Feb 2011 08:56:20 -0600 From: Subject: RE: [Histonet] Wage question for Wisconsin To: , Message-ID: Content-Type: text/plain; charset="US-ASCII" At least you have a union!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, February 15, 2011 6:48 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Wage question for Wisconsin The answer to that question may have to wait a week or two. At least here at the UW. Our wonderful governor is trying to get rid of the state workers unions and collective bargaining. He is trying to push it through (it was just introduced this last Friday), and the vote is slated for later this week. The techs at the UW hospital have fortunately signed their contract that is good until the reopener in 1013 and we are not strictly state employees. However, the way things are looking, it is not out of the question that he may also try to void our contracts. Our pay MAY go up, but we will no longer have much, if anything, for benefits. Don't count on sending your kids to the UW colleges if this goes through. Sorry, I'm fighting mad right now. I'm not usually big on the unions, but this is HUGE for millions of Wisconsinites. GGRRRRR! Claire Were there any other Wisconsin techs at the rally today? Even the Police and Firefighters unions were down there! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Michael Hillmer Sent: Tue 2/15/2011 5:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wage question for Wisconsin We are dermatology clinic in Northeast Wisconsin and we are trying to do gather accurate wage data. Can anybody offer wages for an HT, HtL and lab assistants? Any help would be greatly appreciated. Thank you- Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 16 Feb 2011 08:59:44 -0600 From: Subject: RE: [Histonet] Section position on slides To: , Message-ID: Content-Type: text/plain; charset="US-ASCII" I learned from an old school HT and studied using the Carson book. In this book there are several pages on where to put sections. If you don't have the Frieda Carson book (or "the bible" as I refer to it), get it. A new edition just came out about a year ago. If you're an ASCP member I think the price is reduced... For the most part, just put everything as close to the middle of the clear glass part of the slide as possible. This also does well for IHC automation. I think most automated machines you can adjust the drop range too if you want to put the sections on the bottom or top? Good Luck! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Tuesday, February 15, 2011 11:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. "x" mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 16 Feb 2011 10:15:29 -0500 From: "Gill, Caula A." Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis To: "Ingles Claire " , Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9F38@MDGEN-EXCH1.marylandgeneral.org> Content-Type: text/plain; charset="us-ascii" I agree, gum sounds better not to mention the taste CG -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, February 15, 2011 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis Wouldn't GUM be a more palatable option? Who knows where some of those rubber bands have been! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Mon 2/14/2011 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: saliva for glycogen hydrolysis Here's a truly hi-tech suggestion for doing PAS-diastase stains by the spit method (which by the way is still how it's done by the small pathology services I work on): You can produce copious quantities of saliva by the simple expedient of chewing on a rubber band for a couple of minutes. This used to be the technique used to obtain saliva samples for determination of ABH substance secretor status in the blood bank. (I belong to that elite 20% of donors who are non-secretors, and Lewis-a positive to prove it.) Bob Richmond Samurai pathologist and occasional sialogogue Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 16 Feb 2011 10:25:27 -0500 From: Victoria Baker Subject: Re: [Histonet] Wage question for Wisconsin To: Michael Hillmer Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Michael, ASCP has a wage/salary survey that they distribute based upon region and certification. Some have said that this is biased as not everyone does participate. I tend to agree with the survey, but also recognize that dermatopathology is a 'specialty' type lab. I worked in Dermpath at NYU back in the 80's to early 90's and our salaries were not identical to the clinical labs as we were a part of the medical school and not the hospital. Yes the hospital was 1199 at that time, I cannot say if they are still. My recommendation is to go with what you can find from the survey, but also network with other dermpath or other specialty labs in your area that you may know or who you can connect with through others - either on the listserver or off - to possibly get a better sounding of salaries. HR at your hospital might be also able to help if you have someone there that you can work with and trust to obtain some of the data you're looking for and understands what your specific needs are within the lab. I would include your pathologists in this as much as possible as they will be needed to support your request(s) as they are essentially your clients. Vikki On Tue, Feb 15, 2011 at 6:02 PM, Michael Hillmer wrote: > We are dermatology clinic in Northeast Wisconsin and we are trying to do > gather accurate wage data. Can anybody offer wages for an HT, HtL and > lab assistants? Any help would be greatly appreciated. > > > > > > > > > > Thank you- > > > > Michael Hillmer PHR > > HR Coordinator > > Dermatology Associates of Wisconsin > > Phone: (920)683-5278 > > Fax: (920)686-9674 > > Cell: (920)860-6360 > > > > The materials and information in this e-mail are confidential and may > contain Protected Health Information covered under the HIPAA Privacy > Rule. If you are not the intended recipient, be advised that any > unauthorized use, disclosure, copying, distribution, or action taken in > reliance on the contents of this information is strictly forbidden by > law. If you have received this e-mail in error, please notify me by > reply e-mail and then delete this message. Do not pass any of this > information to anyone else. Thank you for your cooperation. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 15 Date: Wed, 16 Feb 2011 10:27:55 -0500 From: "Shirley A. Powell" Subject: [Histonet] Correction, Correction on cutoff date for GSH meeting To: "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE22DBB3A7DE3@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" Correction: There is a correction on the cutoff date for the hotel reservations. Cutoff date for discounted room rate is February 23rd, Not March 1st. There are several other events going on that weekend and the hotel is filling up fast. Please make your reservations and register soon so you will not miss out on the great room rate and a great meeting. Shirley Powell Hi Georgia, Alabama, ALL histotechs, The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge. The deadline for making hotel reservations is March 1, 2011 so that gives you a month to make your plans to attend, don't delay. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The complete program can be downloaded from our website at this link: www.histosearch.com/gsh> then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form. The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance. Come TRANSFORM yourselves. Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ------------------------------ Message: 16 Date: Wed, 16 Feb 2011 08:59:36 -0700 From: "Breeden, Sara" Subject: [Histonet] Pathco double-blade handle To: "Histonet" Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4766A@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Am looking for this item to replace one that was older than I am. I need the handle (perhaps Pathco #D2877) for the blades (Pathco #D2878-2C). The catch is that I want the SOLID BRASS handle - not the aluminum. I've "googled" all over the place - can anyone help me find these handles? Muchas gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ Message: 17 Date: Wed, 16 Feb 2011 11:09:31 -0500 From: "jstaruk" Subject: RE: [Histonet] Pathco double-blade handle To: "'Breeden, Sara'" , "'Histonet'" Message-ID: <005501cbcdf3$e623bd40$b26b37c0$@masshistology.com> Content-Type: text/plain; charset="us-ascii" I've found two brass handles on Ebay in the last few years. I don't know if you're aware of this but the exact same blades that fit these handles can be purchased at Home Depot in the carpet section. $10 for 50 of them. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, February 16, 2011 11:00 AM To: Histonet Subject: [Histonet] Pathco double-blade handle Am looking for this item to replace one that was older than I am. I need the handle (perhaps Pathco #D2877) for the blades (Pathco #D2878-2C). The catch is that I want the SOLID BRASS handle - not the aluminum. I've "googled" all over the place - can anyone help me find these handles? Muchas gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1204 / Virus Database: 1435/3447 - Release Date: 02/16/11 ------------------------------ Message: 18 Date: Wed, 16 Feb 2011 11:12:00 -0500 From: "caymanfleck@gmail.com" Subject: [Histonet] Exam Prep To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello Histoland, I'm preparing for the HTL exam after being trained on-the-job and working in histo for a few years. I'm using the standard 'Theory and practice...' text as my main resource. I'm wondering if anyone has used either the ASCP practice tests (a set of 5 are available from ascp for about $30) or the NSH Self-assessment Program (which has modules that correspond to the exam sections). The NSH program is expensive at about $240 but I don't know if it's worth it. Has anyone used either of these resources? Thanks! ------------------------------ Message: 19 Date: Wed, 16 Feb 2011 10:23:52 -0600 From: "Johnson, Nacaela" Subject: RE: [Histonet] Exam Prep To: , Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D539@txhous1eb012.uson.usoncology.int> Content-Type: text/plain; charset="us-ascii" I just used the Freida Carson Histotechnology A Self-Instructional Test. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caymanfleck@gmail.com Sent: Wednesday, February 16, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exam Prep Hello Histoland, I'm preparing for the HTL exam after being trained on-the-job and working in histo for a few years. I'm using the standard 'Theory and practice...' text as my main resource. I'm wondering if anyone has used either the ASCP practice tests (a set of 5 are available from ascp for about $30) or the NSH Self-assessment Program (which has modules that correspond to the exam sections). The NSH program is expensive at about $240 but I don't know if it's worth it. Has anyone used either of these resources? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. ------------------------------ Message: 20 Date: Wed, 16 Feb 2011 10:35:21 -0600 From: Subject: RE: [Histonet] Exam Prep To: , , Message-ID: Content-Type: text/plain; charset="US-ASCII" If you basically memorize this book, and then have a pretty good understanding of IHC...then you should do fine. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela Sent: Wednesday, February 16, 2011 10:24 AM To: caymanfleck@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Exam Prep I just used the Freida Carson Histotechnology A Self-Instructional Test. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caymanfleck@gmail.com Sent: Wednesday, February 16, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exam Prep Hello Histoland, I'm preparing for the HTL exam after being trained on-the-job and working in histo for a few years. I'm using the standard 'Theory and practice...' text as my main resource. I'm wondering if anyone has used either the ASCP practice tests (a set of 5 are available from ascp for about $30) or the NSH Self-assessment Program (which has modules that correspond to the exam sections). The NSH program is expensive at about $240 but I don't know if it's worth it. Has anyone used either of these resources? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 16 Feb 2011 11:08:07 -0600 From: "Bill B." Subject: Re: [Histonet] Section position on slides To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" As a pathologist I have some preferences on how certain tissues are oriented on a slide and work with my histotechs so they can learn my prefs. I also like my slides in certain orientation in the slide tray. EG, for orientation of skin, I like the the dermis at the top when I am looking at the slide. Some other tissues can be so oriented, some cannot. We often put multiple levels on one slide and I would prefer them to be all oriented similarly and in a straight line. The most annoying thing I see beyond coverslipping the wrong side, is the label covering a part of the specimen. I don't care how close to the edge a section is as long as it is all on the slide and covered by the cover-slip or mounting medium. I have no idea is these preferences are weird. My point is the answer to you 1st question may depend on your particular pathologists' preferences. BIll Blank At 10:09 PM -0700 2/15/11, Tanya Ewing-Finchem wrote: >1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. ?x? mm from the edge of the glass slide)? ------------------------------ Message: 22 Date: Wed, 16 Feb 2011 17:20:57 +0000 From: "Bartlett, Jeanine (CDC/OID/NCEZID)" Subject: RE: [Histonet] Section position on slides To: Tanya Ewing-Finchem , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I always try to center the section equally from sides and top-bottom as possible. This means measure from the bottom of the frosted edge as the "top". The Artisan special stains system has a clip that attaches around the slide to allow reagents to pool onto the sections and incubate. If the section is too close to the sides then these areas do not stain adequately. Sometimes if the tissue section itself is very large this is unavoidable. With automated coverslippers you must also consider placement of tissue to allow for proper coverage. If I am cutting multiple unstained slides for subsequent testing I try to orient the tissue the same on each slide to facilitate the reading of these slides by the pathologist's Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Wednesday, February 16, 2011 12:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. "x" mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Wed, 16 Feb 2011 12:27:32 -0500 From: Helen Fedor Subject: RE: [Histonet] Section position on slides To: "Bartlett, Jeanine (CDC/OID/NCEZID)" , Tanya Ewing-Finchem , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Since automation is becoming more and more a part of all Histology labs the demands of placement of the tissue on the slides varies for different instruments. Stainers, coverslippers and now with slide scanning as well. So I do not believe that there is a silver bullet answer. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 16, 2011 12:21 PM To: Tanya Ewing-Finchem; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Section position on slides I always try to center the section equally from sides and top-bottom as possible. This means measure from the bottom of the frosted edge as the "top". The Artisan special stains system has a clip that attaches around the slide to allow reagents to pool onto the sections and incubate. If the section is too close to the sides then these areas do not stain adequately. Sometimes if the tissue section itself is very large this is unavoidable. With automated coverslippers you must also consider placement of tissue to allow for proper coverage. If I am cutting multiple unstained slides for subsequent testing I try to orient the tissue the same on each slide to facilitate the reading of these slides by the pathologist's Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Wednesday, February 16, 2011 12:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. "x" mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 87, Issue 28 **************************************** This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From gilsharo <@t> post.tau.ac.il Thu Feb 17 08:46:04 2011 From: gilsharo <@t> post.tau.ac.il (Gil Sharon) Date: Thu Feb 17 08:46:11 2011 Subject: [Histonet] Drosophila Sectioning Message-ID: <4BD4759CE4D84480B0D73E317744289B@danas> Hi All, As a microbial ecologist, working with drosophila, I'm looking for a couple of protocols (or papers) for fixation and paraffin-embedding of embryos, 3rd instar larvae, pupae and adult D. melanogaster (these sections will later be used for fluresecnt-in-situ-hybridization probing for specific bacteria). Looking in the literature, I could only find protocols for embryos (or fly heads). As I'm really new to histology, I hope someone here will be able to help me with this. I have the help of a histo lab here, but they're working on mice, so no experience with drosophila (or insects at all). Thanks in advance, Gil. From pruegg <@t> ihctech.net Thu Feb 17 08:49:53 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Feb 17 08:50:31 2011 Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section In-Reply-To: <544851.36245.qm@web38005.mail.mud.yahoo.com> References: <544851.36245.qm@web38005.mail.mud.yahoo.com> Message-ID: <3A5E8FD47DF948B888D5F8765F834905@prueggihctechlt> I do not know of a neutrophil specific ab for human ffpe tissue. You could do just DAB without blocking with h202 for myleoperoxidase but it will not distinguish between eosinophils and neutrophils myeloperoxidase will stain all myeloid cells with granules in the cytoplasm (granulocytes). Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Wednesday, February 16, 2011 1:07 PM To: histonet Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. ? Could anybody recommend a good antibody? ? Thanks in advance, ? Amy ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Feb 17 09:05:59 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Feb 17 09:06:57 2011 Subject: [Histonet] Drosophila Sectioning In-Reply-To: <4BD4759CE4D84480B0D73E317744289B@danas> References: <4BD4759CE4D84480B0D73E317744289B@danas> Message-ID: <68DB5793-A0F9-4143-AAB5-12C82E05A51D@email.arizona.edu> I have done whiteflies, mosquitoes, honey bees and cockroaches among other insects and insect parts. If you contact me offline maybe I can help you. Andi Grantham On Feb 17, 2011, at 7:46 AM, Gil Sharon wrote: > Hi All, > > > > As a microbial ecologist, working with drosophila, I'm looking for a couple > of protocols (or papers) for fixation and paraffin-embedding of embryos, 3rd > instar larvae, pupae and adult D. melanogaster (these sections will later be > used for fluresecnt-in-situ-hybridization probing for specific bacteria). > Looking in the literature, I could only find protocols for embryos (or fly > heads). As I'm really new to histology, I hope someone here will be able to > help me with this. I have the help of a histo lab here, but they're working > on mice, so no experience with drosophila (or insects at all). > > > > Thanks in advance, > > Gil. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lbustamante <@t> cvm.tamu.edu Thu Feb 17 09:39:37 2011 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Thu Feb 17 09:40:23 2011 Subject: [Histonet] Zinc Formalin and IHC Message-ID: <4D5CECD9020000B9000F4C26@CVM.TAMU.EDU> Posting for a friend. Replay to shernandez@st-joseph.org Is there any negative effect on IHC and Special Stain with the use of buffered Zinc Formalin? Thank you. Susan Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 From dlaudier <@t> gmail.com Thu Feb 17 10:31:45 2011 From: dlaudier <@t> gmail.com (Damien Laudier) Date: Thu Feb 17 10:31:49 2011 Subject: [Histonet] Drosophila sectioning Message-ID: Hi Gil, My histology service specializes in insects & related invertebrates. I have a protocol that I can send you & answer any additional questions you may have. Please feel free to contact me. Best, Damien Laudier From shive003 <@t> umn.edu Thu Feb 17 10:39:29 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Feb 17 10:39:35 2011 Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section References: <544851.36245.qm@web38005.mail.mud.yahoo.com> <3A5E8FD47DF948B888D5F8765F834905@prueggihctechlt> Message-ID: Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE tissues, according to their website. Jan Shivers ----- Original Message ----- From: "Patsy Ruegg" To: "'Amy Lee'" ; "'histonet'" Sent: Thursday, February 17, 2011 8:49 AM Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section I do not know of a neutrophil specific ab for human ffpe tissue. You could do just DAB without blocking with h202 for myleoperoxidase but it will not distinguish between eosinophils and neutrophils myeloperoxidase will stain all myeloid cells with granules in the cytoplasm (granulocytes). Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Wednesday, February 16, 2011 1:07 PM To: histonet Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. Could anybody recommend a good antibody? Thanks in advance, Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Thu Feb 17 12:00:47 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Feb 17 12:10:46 2011 Subject: [Histonet] I Message-ID: I will be out of the office starting 02/17/2011 and will not return until 02/21/2011. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. From pruegg <@t> ihctech.net Thu Feb 17 12:20:57 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Feb 17 12:21:31 2011 Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section In-Reply-To: References: <544851.36245.qm@web38005.mail.mud.yahoo.com> <3A5E8FD47DF948B888D5F8765F834905@prueggihctechlt> Message-ID: <4118E59E011945E589BB6D9076C9BAD3@Patsyoffice> Does it also work on human? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Jan Shivers [mailto:shive003@umn.edu] Sent: Thursday, February 17, 2011 9:39 AM To: Patsy Ruegg; 'Amy Lee'; 'histonet' Subject: Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE tissues, according to their website. Jan Shivers ----- Original Message ----- From: "Patsy Ruegg" To: "'Amy Lee'" ; "'histonet'" Sent: Thursday, February 17, 2011 8:49 AM Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section I do not know of a neutrophil specific ab for human ffpe tissue. You could do just DAB without blocking with h202 for myleoperoxidase but it will not distinguish between eosinophils and neutrophils myeloperoxidase will stain all myeloid cells with granules in the cytoplasm (granulocytes). Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Wednesday, February 16, 2011 1:07 PM To: histonet Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. Could anybody recommend a good antibody? Thanks in advance, Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmlaud <@t> gmail.com Thu Feb 17 09:33:59 2011 From: dmlaud <@t> gmail.com (Damien) Date: Thu Feb 17 13:05:41 2011 Subject: [Histonet] Re: Drosophila sectioning Message-ID: Hi Gil, My histology service specializes in insects. I have a protocol that I can send you & answer any additional questions you may have. Please feel free to contact me. Best, Damien Laudier On Thu, Feb 17, 2011 at 9:52 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Section position on slides (Victoria Baker) > 2. Information Please (Jesus Ellin) > 3. Ventana Rep. (DKBoyd@chs.net) > 4. RE: Exam Prep (Rochan Moir-Dial) > 5. Colorado Sate Histo Meeting, April 29-30, 2011 (Matthew Lunetta) > 6. NY lab permit (Marian Powers) > 7. neutrophil antibody on rat FFP section (Amy Lee) > 8. CellSoft vs. Sodium Hydroxide (traczyk7@aol.com) > 9. Re: saliva for glycogen hydrolysis (Eric Hoy) > 10. Re: Novolink polymer kit (amitapandey@torrentpharma.com) > 11. RE: Histonet Digest, Vol 87, Issue 28 (Ma, Minhui) > 12. Drosophila Sectioning (Gil Sharon) > 13. RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP > section (Patsy Ruegg) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 16 Feb 2011 13:23:02 -0500 > From: Victoria Baker > Subject: Re: [Histonet] Section position on slides > To: Helen Fedor > Cc: Tanya Ewing-Finchem , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Automated coverslipping instruments are defined by the manufacturer, but > modifications on some systems can be done. Most clinical labs who utilize > automation use 24X50mm - as this coverslip will cover most of the slide. > > Tissue placement is pretty much determined by laboratory needs. Sheehan & > Hrapchak had a short segment about tissue placement, but it was in regards > to the tissue orientation for microscopic assessment requirements. There > is > nothing in granite about tissue placement that I know of. > > Some tools that I worked with were using the Cytospin large rectangular > slides to show residents/students/collegues where the tissue placement > needed to be. Once they were able to get the tissue section in there we > moved on. While to most of us who work in Histo sort of take putting > sections on a slide for granted (yes we did work hard to get there) > getting > the mechanics of it takes patience, skill and practice. Keep it simple and > build up from there. > > Vikki > > > On Wed, Feb 16, 2011 at 12:27 PM, Helen Fedor wrote: > > > Since automation is becoming more and more a part of all Histology labs > the > > demands of placement of the tissue on the slides varies for different > > instruments. Stainers, coverslippers and now with slide scanning as well. > So > > I do not believe that there is a silver bullet answer. > > > > Helen L. Fedor > > > > Tissue Microarray Lab, Manager > > Prostate Spore Lab, Manager > > Johns Hopkins University > > 600 N. Wolfe St, | Marburg Room 406 > > Baltimore, MD | 21287-7065 > > > > 410.614.1660 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, > Jeanine > > (CDC/OID/NCEZID) > > Sent: Wednesday, February 16, 2011 12:21 PM > > To: Tanya Ewing-Finchem; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Section position on slides > > > > I always try to center the section equally from sides and top-bottom as > > possible. This means measure from the bottom of the frosted edge as the > > "top". The Artisan special stains system has a clip that attaches around > > the slide to allow reagents to pool onto the sections and incubate. If > the > > section is too close to the sides then these areas do not stain > adequately. > > Sometimes if the tissue section itself is very large this is > unavoidable. > > With automated coverslippers you must also consider placement of tissue > to > > allow for proper coverage. > > > > If I am cutting multiple unstained slides for subsequent testing I try to > > orient the tissue the same on each slide to facilitate the reading of > these > > slides by the pathologist's > > > > Jeanine Bartlett > > Infectious Diseases Pathology Branch > > (404) 639-3590 > > jeanine.bartlett@cdc.hhs.gov > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya > > Ewing-Finchem > > Sent: Wednesday, February 16, 2011 12:09 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Section position on slides > > > > > > I am trying to put together a training document around microtomy and > > sectioning and am finding it hard to find information around the > placement > > of the actual sections on the slides. These are the objectives I am > looking > > to answer. Is this information found in any publications? > > > > 1) Tissue / Section Placement: Are there published guidelines / > > documentation on precisely where you should place tissue sections on a > 25mm > > x 75mm glass slide? Perhaps more importantly, where you should NOT > place > > tissue (ie. "x" mm from the edge of the glass slide)? > > > > 2) Diagnosable Slide Staining Area: With automation becoming more > widely > > used in IHC, are there published guidelines / documentation on the usable > or > > diagnosable staining area on a 25mm x 75mm glass slide? For instance, > would > > you define that as the area under a traditional coverslip? Would this be > > defined as the entire slide below the label? Or is this some distance > from > > all the edges of the slide? With some automated systems, it is near > > impossible to get edge to edge staining. Is this acceptable? > > > > > > > > Thanks for any ideas. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Wed, 16 Feb 2011 11:38:04 -0700 > From: "Jesus Ellin" > Subject: [Histonet] Information Please > To: "histonet" > Message-ID: > > <29BE166A2CF48D459853F8EC57CD37E8035D68ED@EXCHANGECLUSTER.yumaregional.local > > > > Content-Type: text/plain; charset="us-ascii" > > Wanting to get information on anyone that has already implemented Epic > and offered the Epic ambulatory piece to there clients. Are you also > offering this technology with interface to competitive laboratories > like, Quest, Lab Corp, etc? > > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > ------------------------------ > > Message: 3 > Date: Wed, 16 Feb 2011 13:39:22 -0500 > From: DKBoyd@chs.net > Subject: [Histonet] Ventana Rep. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Can a Ventana Representative for the Southern Virginia region, please give > me a call at the number below. > Please only a Ventana rep. I do not have authorization to deal with > another company at this time. > Thank you. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkboyd@chs.net > > > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > ------------------------------ > > Message: 4 > Date: Wed, 16 Feb 2011 11:47:51 -0700 > From: "Rochan Moir-Dial" > Subject: RE: [Histonet] Exam Prep > To: "Johnson, Nacaela" , > , > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I also used Freida Carson, Histotechnology A Self-Instructional Test, > 3rd Edition. As well as, the Freida Carson, Companion Study Flash Cards > that goes along with the 3rd Edition text book. Study the objectives and > your golden. > > Rochan Moir-Dial, HTL(ASCP) > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > Phone: (303) 682-3949 > Fax (303) 682-9060 > rochan@premierlab.com > www.premierlab.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, > Nacaela > Sent: Wednesday, February 16, 2011 9:24 AM > To: caymanfleck@gmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Exam Prep > > I just used the Freida Carson Histotechnology A Self-Instructional Test. > > > > Thanks, > > Nacaela Johnson, HTL (ASCP) > Histotechnologist > KCCC Pathology > 12000 110th St., Ste. 400 > Overland Park, KS 66210 > Office: 913-234-0576 > Fax: 913-433-7639 > Email: Nacaela.Johnson@USOncology.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > caymanfleck@gmail.com > Sent: Wednesday, February 16, 2011 10:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Exam Prep > > Hello Histoland, > > I'm preparing for the HTL exam after being trained on-the-job and > working in histo for a few years. I'm using the standard 'Theory and > practice...' text as my main resource. I'm wondering if anyone has used > either the ASCP practice tests (a set of 5 are available from ascp for > about $30) or the NSH Self-assessment Program (which has modules that > correspond to the exam sections). The NSH program is expensive at about > $240 but I don't know if it's worth it. Has anyone used either of these > resources? > > Thanks! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > The contents of this electronic mail message and any attachments > are confidential, possibly privileged and intended for the addressee(s) > only.
Only the addressee(s) may read, disseminate, retain or > otherwise use this message. If received in error, please immediately > inform the sender and then delete this message without disclosing its > contents to anyone. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Wed, 16 Feb 2011 12:16:30 -0700 > From: "Matthew Lunetta" > Subject: [Histonet] Colorado Sate Histo Meeting, April 29-30, 2011 > To: > Message-ID: <4D5BC01E020000A800056647@ns.luhcares.org> > Content-Type: text/plain; charset=US-ASCII > > Hi, > > All are welcome. It is a great program this year. > > Attached is the program for the 2011 CSH meeting, which will be held April > 29th & 30th at the Embassy Suites hotel in Loveland, CO. Online registration > and credit card payment is available on the CSH website at > http://www.coloradohisto.org/2011/meeting.htm > > Ciao > Matt Lunetta > HT (ASCP) > > > ------------------------------ > > Message: 6 > Date: Wed, 16 Feb 2011 14:18:23 -0500 > From: Marian Powers > Subject: [Histonet] NY lab permit > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello all, > > Anyone out there have experience in applying for a NY state lab permit? > How > long did it take? How difficult was it? Could anyone recommend a lab > consultant on this task? > > Thanks in advance, > > > > -- > *Marian L. Powers, BSOM, HT(ASCP) * > > * * > * * > * * > > > ------------------------------ > > Message: 7 > Date: Wed, 16 Feb 2011 12:06:41 -0800 (PST) > From: Amy Lee > Subject: [Histonet] neutrophil antibody on rat FFP section > To: histonet > Message-ID: <544851.36245.qm@web38005.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello, > I was asked to perform neutrophil IHC on rat FFP sections. I searched > around and found most are for mouse tissue. There is one from Serotec mouse > anti rat but it does not work on paraffin section. > > Could anybody recommend a good antibody? > > Thanks in advance, > > Amy > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 16 Feb 2011 16:31:36 -0500 (EST) > From: traczyk7@aol.com > Subject: [Histonet] CellSoft vs. Sodium Hydroxide > To: histonet@lists.utsouthwestern.edu > Message-ID: <8CD9C38063E34B6-12F8-6B35@webmail-d064.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > Greetings one & all: > Has anyone tried "CellSoft" from CellPath in the UK? I received a sample > today and after reviewing the MSDS that came with it, I'm skeptical about > the claim in their literature that it "reduces the hazard risk for the > user". I've got 10% sodium hydroxide on hand for softening nails so if > anyone has already done a product comparison, I'd love to hear from you. > Thanks. > Dorothy > > > > > > > > ------------------------------ > > Message: 9 > Date: Wed, 16 Feb 2011 15:33:23 -0600 > From: Eric Hoy > Subject: [Histonet] Re: saliva for glycogen hydrolysis > To: Histonet , > "histonet-request@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > > From: "Ingles Claire " > > Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis > > > Wouldn't GUM be a more palatable option? Who knows where some of those > rubber > > bands have been! > > Claire > > > > Back in my early days in the lab, when I was in the US Navy (wooden ships > and Folin-Wu glucose), I was involved in a project that required us to > collect fairly large (10-15 mL) saliva samples from Navy recruits who were > part of a research study. We used a wax gum, which I believe was > manufactured by Wrigley's gum company. The sticks were the same size and > shape as gum (back then gum looked like a microscope slide, not all the > shapes that are currently available) and they were individually wrapped > like > gum. They tasted like paraffin, but they induced copious salivation, and > served our purpose well. > > I don't know if this product is still available. > > Eric Hoy > > =============================================== > Eric S. Hoy, Ph.D., SI(ASCP) > Clinical Associate Professor > Department of Medical Laboratory Sciences > The University of Texas Southwestern Medical Center > Dallas, Texas > Email: Eric.Hoy@UTSouthwestern.edu > =============================================== > > > > > > ------------------------------ > > Message: 10 > Date: Thu, 17 Feb 2011 09:43:59 +0530 > From: amitapandey@torrentpharma.com > Subject: [Histonet] Re: Novolink polymer kit > To: "MaxVision Biosciences Inc. " , > Histonet@lists.utsouthwestern.edu > Message-ID: > < > OF9E0253B7.324114FD-ON6525783A.0016B216-6525783A.001723E9@torrentpharma.com > > > > Content-Type: text/plain; charset="US-ASCII" > > Thanks Michelle and all histonetters for your suggestions. > > After your feed back, i am finalizing the Novolink Polymer-HRP detection > kit for my work. I am going to use this kit for single labelling of the > antibody. i will let u know about my result as it comes. > > Amita > > ------------------------------ > > Message: 11 > Date: Thu, 17 Feb 2011 09:33:25 -0500 > From: "Ma, Minhui" > Subject: [Histonet] RE: Histonet Digest, Vol 87, Issue 28 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 43DEC30DB656564EB330190305519FB701ADFE534CAF@EXCHANGE22.Enterprise.emory.net > > > > Content-Type: text/plain; charset="us-ascii" > > Dear everyone, > > I'm wondering where I can order the Picro-Mallory Trichrome Stain kit? I > couldn't find it online excepted some Italian companies. > Thanks, > > Minhui Ma MD,HTL > Emory University > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: Wednesday, February 16, 2011 12:30 PM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 87, Issue 28 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Hema 3/Diff Quik (Wanda.Smith@HCAhealthcare.com) > 2. HT Position in Oklahoma City (Gaiser, Marcia) > 3. RE: Re: saliva for glycogen hydrolysis (Ingles Claire ) > 4. Wage question for Wisconsin (Michael Hillmer) > 5. RE: Wage question for Wisconsin (Ingles Claire ) > 6. Re: Wage question for Wisconsin (Lee & Peggy Wenk) > 7. Section position on slides (Tanya Ewing-Finchem) > 8. Novolink Polymer detection kit (amitapandey@torrentpharma.com) > 9. Re: Section position on slides (Paula Sicurello) > 10. Na K ATpase immunohistochemistry on rat FFPE tissue > (amitapandey@torrentpharma.com) > 11. RE: Wage question for Wisconsin (sgoebel@mirnarx.com) > 12. RE: Section position on slides (sgoebel@mirnarx.com) > 13. RE: Re: saliva for glycogen hydrolysis (Gill, Caula A.) > 14. Re: Wage question for Wisconsin (Victoria Baker) > 15. Correction, Correction on cutoff date for GSH meeting > (Shirley A. Powell) > 16. Pathco double-blade handle (Breeden, Sara) > 17. RE: Pathco double-blade handle (jstaruk) > 18. Exam Prep (caymanfleck@gmail.com) > 19. RE: Exam Prep (Johnson, Nacaela) > 20. RE: Exam Prep (sgoebel@mirnarx.com) > 21. Re: Section position on slides (Bill B.) > 22. RE: Section position on slides > (Bartlett, Jeanine (CDC/OID/NCEZID)) > 23. RE: Section position on slides (Helen Fedor) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 15 Feb 2011 12:07:09 -0600 > From: > Subject: [Histonet] RE: Hema 3/Diff Quik > To: , > Message-ID: > < > 9E2D36CE2D7CBA4A94D9B22E8328A3BA139EE804DD@NADCWPMSGCMS03.hca.corpad.net> > > Content-Type: text/plain; charset="us-ascii" > > Good Afternoon, > We use: > 88333 Touch prep initial site > 88334 Touch prep addition site > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole > Sent: Tuesday, February 15, 2011 12:27 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Hema 3/Diff Quik > > I should also include that we are using this diff quik as a rapid stain > during frozen section in conjunction with a rapid H&E. > > Nicole Anne Konop BS, HTL(ASCP) > Histology Team Lead > Children's Hospital of Wisconsin > (414)266-6580 Direct Line > (414)907-0366 Pager > (414)266-2524 Histology Department > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole > Sent: Tuesday, February 15, 2011 11:01 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Hema 3/Diff Quik > > I'm looking for information on what CPT codes are being used for the diff > quik/hema 3 stain? Any feedback is greatly appreciated. Thanks! > > Nicole Anne Konop BS, HTL(ASCP) > Histology Team Lead > Children's Hospital of Wisconsin > (414)266-6580 Direct Line > (414)907-0366 Pager > (414)266-2524 Histology Department > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Tue, 15 Feb 2011 14:25:18 -0600 > From: "Gaiser, Marcia" > Subject: [Histonet] HT Position in Oklahoma City > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 728F817C02110E498D803A7C3B0C6248057D85BD5C@S009-APEXM06.ds.ad.ssmhc.com> > > Content-Type: text/plain; charset="Windows-1252" > > St. Anthony Hospital currently has an excellent opportunity for an > experienced Histologic Technician. This position requires Certification as > an HT or HLT ? or ? experience acceptable to the Laboratory Director. Two > years of previous histology experience required with IHC and/or grossing > experience a plus. Outstanding benefits package, including generous paid > time off. For consideration, please apply online at www.saintsok.com< > http://www.saintsok.com/ >>, Ad # 10762, or > contact Anna King for additional information at (405) 272-6105. > > > > Confidentiality Notice: This email message, including any > attachments, is for the sole use of the intended recipient(s) and > may contain confidential and privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by > reply email and destroy all copies of the original message. > > ------------------------------ > > Message: 3 > Date: Tue, 15 Feb 2011 16:55:24 -0600 > From: "Ingles Claire " > Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis > To: > Message-ID: > < > F2F030053F9B7345831BED293A6D57E103A1A6CE@UWHC-MAIL01.uwhis.hosp.wisc.edu> > > Content-Type: text/plain; charset="iso-8859-1" > > Wouldn't GUM be a more palatable option? Who knows where some of those > rubber bands have been! > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert > Richmond > Sent: Mon 2/14/2011 11:09 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: saliva for glycogen hydrolysis > > > > Here's a truly hi-tech suggestion for doing PAS-diastase stains by the > spit method (which by the way is still how it's done by the small > pathology services I work on): > > You can produce copious quantities of saliva by the simple expedient > of chewing on a rubber band for a couple of minutes. This used to be > the technique used to obtain saliva samples for determination of ABH > substance secretor status in the blood bank. (I belong to that elite > 20% of donors who are non-secretors, and Lewis-a positive to prove > it.) > > Bob Richmond > Samurai pathologist and occasional sialogogue > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 4 > Date: Tue, 15 Feb 2011 17:02:58 -0600 > From: "Michael Hillmer" > Subject: [Histonet] Wage question for Wisconsin > To: > Message-ID: > < > 9060F1C2EB3D8A4AAF3B3433C9003230F975A8@hostexchbe1.hosting.tushausweb.com> > > Content-Type: text/plain; charset="us-ascii" > > We are dermatology clinic in Northeast Wisconsin and we are trying to do > gather accurate wage data. Can anybody offer wages for an HT, HtL and > lab assistants? Any help would be greatly appreciated. > > > > > > > > > > Thank you- > > > > Michael Hillmer PHR > > HR Coordinator > > Dermatology Associates of Wisconsin > > Phone: (920)683-5278 > > Fax: (920)686-9674 > > Cell: (920)860-6360 > > > > The materials and information in this e-mail are confidential and may > contain Protected Health Information covered under the HIPAA Privacy > Rule. If you are not the intended recipient, be advised that any > unauthorized use, disclosure, copying, distribution, or action taken in > reliance on the contents of this information is strictly forbidden by > law. If you have received this e-mail in error, please notify me by > reply e-mail and then delete this message. Do not pass any of this > information to anyone else. Thank you for your cooperation. > > > > > > ------------------------------ > > Message: 5 > Date: Tue, 15 Feb 2011 18:48:09 -0600 > From: "Ingles Claire " > Subject: RE: [Histonet] Wage question for Wisconsin > To: > Message-ID: > < > F2F030053F9B7345831BED293A6D57E103A1A6D2@UWHC-MAIL01.uwhis.hosp.wisc.edu> > > Content-Type: text/plain; charset="iso-8859-1" > > > The answer to that question may have to wait a week or two. At least here > at the UW. Our wonderful governor is trying to get rid of the state workers > unions and collective bargaining. He is trying to push it through (it was > just introduced this last Friday), and the vote is slated for later this > week. The techs at the UW hospital have fortunately signed their contract > that is good until the reopener in 1013 and we are not strictly state > employees. However, the way things are looking, it is not out of the > question that he may also try to void our contracts. Our pay MAY go up, but > we will no longer have much, if anything, for benefits. Don't count on > sending your kids to the UW colleges if this goes through. > Sorry, I'm fighting mad right now. I'm not usually big on the unions, but > this is HUGE for millions of Wisconsinites. GGRRRRR! > Claire > > Were there any other Wisconsin techs at the rally today? Even the Police > and Firefighters unions were down there! > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Michael > Hillmer > Sent: Tue 2/15/2011 5:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wage question for Wisconsin > > > > We are dermatology clinic in Northeast Wisconsin and we are trying to do > gather accurate wage data. Can anybody offer wages for an HT, HtL and > lab assistants? Any help would be greatly appreciated. > > > Thank you- > > > > Michael Hillmer PHR > > HR Coordinator > > Dermatology Associates of Wisconsin > > Phone: (920)683-5278 > > > > > > > ------------------------------ > > Message: 6 > Date: Tue, 15 Feb 2011 20:27:32 -0500 > From: "Lee & Peggy Wenk" > Subject: Re: [Histonet] Wage question for Wisconsin > To: "Michael Hillmer" , > > Message-ID: <75338FFC879A4718B6404F650270FE3C@HP2010> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > The following link is to the ASCP "Laboratory Medicine" article from March > 2009. They do the survey every other year, so there should be another > survey > coming out any month now. > > http://www.ascp.org/pdf/Membership-Communications/Wage-and-Vacancy-Survey.aspx > > Advance for Medical Laboratory Professionals just published their survey on > Jan. 31, 2011. > > http://laboratorian.advanceweb.com/SharedResources/Downloads/2011/013111/MT013111_SalarySurvey.pdf > > Realize that even with a state, there can be several dollars per hour > difference. Let alone the differences between states in a region. I suggest > taking the information in the surveys, look at the relationship between > HT/HTL, MLT/MT, and CT, and then apply the same ratio within your own > institute. > > Hope that helps. > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospital > Royal Oak, MI 48073 > > -------------------------------------------------- > From: "Michael Hillmer" > Sent: Tuesday, February 15, 2011 6:02 PM > To: > Subject: [Histonet] Wage question for Wisconsin > > > We are dermatology clinic in Northeast Wisconsin and we are trying to do > > gather accurate wage data. Can anybody offer wages for an HT, HtL and > > lab assistants? Any help would be greatly appreciated. > > > > > > > > > > > > > > > > > > > > Thank you- > > > > > > > > Michael Hillmer PHR > > > > HR Coordinator > > > > Dermatology Associates of Wisconsin > > > > Phone: (920)683-5278 > > > > Fax: (920)686-9674 > > > > Cell: (920)860-6360 > > > > > > > > The materials and information in this e-mail are confidential and may > > contain Protected Health Information covered under the HIPAA Privacy > > Rule. If you are not the intended recipient, be advised that any > > unauthorized use, disclosure, copying, distribution, or action taken in > > reliance on the contents of this information is strictly forbidden by > > law. If you have received this e-mail in error, please notify me by > > reply e-mail and then delete this message. Do not pass any of this > > information to anyone else. Thank you for your cooperation. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 7 > Date: Tue, 15 Feb 2011 22:09:24 -0700 > From: Tanya Ewing-Finchem > Subject: [Histonet] Section position on slides > To: > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > > I am trying to put together a training document around microtomy and > sectioning and am finding it hard to find information around the placement > of the actual sections on the slides. These are the objectives I am looking > to answer. Is this information found in any publications? > > 1) Tissue / Section Placement: Are there published guidelines / > documentation on precisely where you should place tissue sections on a 25mm > x 75mm glass slide? Perhaps more importantly, where you should NOT place > tissue (ie. ?x? mm from the edge of the glass slide)? > > 2) Diagnosable Slide Staining Area: With automation becoming more widely > used in IHC, are there published guidelines / documentation on the usable or > diagnosable staining area on a 25mm x 75mm glass slide? For instance, would > you define that as the area under a traditional coverslip? Would this be > defined as the entire slide below the label? Or is this some distance from > all the edges of the slide? With some automated systems, it is near > impossible to get edge to edge staining. Is this acceptable? > > > Thanks for any ideas. > > ------------------------------ > > Message: 8 > Date: Wed, 16 Feb 2011 11:39:14 +0530 > From: amitapandey@torrentpharma.com > Subject: [Histonet] Novolink Polymer detection kit > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > OF0B53C2D6.1196A49A-ON65257839.002127B5-65257839.0021CD79@torrentpharma.com > > > > Content-Type: text/plain; charset="US-ASCII" > > Hello Histonetters, > > This is first time, I am planning to use Novolink polymer detection kit > for my mouse monoclonal antibody (Millipore Anti Na K ATPase and anti > vimentin) and rabbit polyclonal antibody (Sc-1506R). > > If any body has experience on this polymer kit , please share your > valuable experience which will help me to set up the method in my lab. > > Thanks in advance > Amita > > ------------------------------ > > Message: 9 > Date: Wed, 16 Feb 2011 07:02:58 -0500 > From: Paula Sicurello > Subject: Re: [Histonet] Section position on slides > To: Tanya Ewing-Finchem > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=windows-1252 > > Hi Tanya, > > It's always best to train people to place the section in the middle of > the slide. It depends if one has to put multiple sections on a slide. > Learning how to do proper placement will help them when they have to > cut controls for IHC that are often times placed on the top of the > slide. > > Sections placed too close to the very bottom or top sometimes don't > get stained or coverslipped. Too close to the edges and you can > suffer wrap around or overlap, causing the section to be difficult for > the pathologist to read. Boy howdy! They will let you know if they > don't like the placement. > > Therefore, train for centering the section as best as possible. > > I hope this helps, > > Paula :-) > > On Wed, Feb 16, 2011 at 12:09 AM, Tanya Ewing-Finchem > wrote: > > > > I am trying to put together a training document around microtomy and > sectioning and am finding it hard to find information around the placement > of the actual sections on the slides. ?These are the objectives I am looking > to answer. ?Is this information found in any publications? > > > > 1) ?Tissue / Section Placement: ?Are there published guidelines / > documentation on precisely where you should place tissue sections on a 25mm > x 75mm glass slide? ? Perhaps more importantly, where you should NOT place > tissue (ie. ?x? mm from the edge of the glass slide)? > > > > 2) ?Diagnosable Slide Staining Area: ?With automation becoming more > widely used in IHC, are there published guidelines / documentation on the > usable or diagnosable staining area on a 25mm x 75mm glass slide? ?For > instance, would you define that as the area under a traditional coverslip? > ?Would this be defined as the entire slide below the label? ?Or is this some > distance from all the edges of the slide? ?With some automated systems, it > is near impossible to get edge to edge staining. ?Is this acceptable? > > > > > Thanks for any ideas. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Electron Microscope Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: ?919.684.2091 > > > > ------------------------------ > > Message: 10 > Date: Wed, 16 Feb 2011 17:42:37 +0530 > From: amitapandey@torrentpharma.com > Subject: [Histonet] Na K ATpase immunohistochemistry on rat FFPE > tissue > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > OFF2BD856F.83823B44-ON65257839.0042A96B-65257839.00431316@torrentpharma.com > > > > Content-Type: text/plain; charset="US-ASCII" > > Does any body have experienced on Anti Na K ATpase immunohistochemistry on > FFPE rat kidney tissue? > I am finding difficult to have any supportive lit regarding this, most of > them support to frozen sectioning. > > Thanks a lot > > Amita > > ------------------------------ > > Message: 11 > Date: Wed, 16 Feb 2011 08:56:20 -0600 > From: > Subject: RE: [Histonet] Wage question for Wisconsin > To: , > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > At least you have a union!! > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Tuesday, February 15, 2011 6:48 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Wage question for Wisconsin > > > The answer to that question may have to wait a week or two. At least > here at the UW. Our wonderful governor is trying to get rid of the state > workers unions and collective bargaining. He is trying to push it > through (it was just introduced this last Friday), and the vote is > slated for later this week. The techs at the UW hospital have > fortunately signed their contract that is good until the reopener in > 1013 and we are not strictly state employees. However, the way things > are looking, it is not out of the question that he may also try to void > our contracts. Our pay MAY go up, but we will no longer have much, if > anything, for benefits. Don't count on sending your kids to the UW > colleges if this goes through. > Sorry, I'm fighting mad right now. I'm not usually big on the unions, > but this is HUGE for millions of Wisconsinites. GGRRRRR! > Claire > > Were there any other Wisconsin techs at the rally today? Even the > Police and Firefighters unions were down there! > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Michael > Hillmer > Sent: Tue 2/15/2011 5:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wage question for Wisconsin > > > > We are dermatology clinic in Northeast Wisconsin and we are trying to do > gather accurate wage data. Can anybody offer wages for an HT, HtL and > lab assistants? Any help would be greatly appreciated. > > > Thank you- > > > > Michael Hillmer PHR > > HR Coordinator > > Dermatology Associates of Wisconsin > > Phone: (920)683-5278 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Wed, 16 Feb 2011 08:59:44 -0600 > From: > Subject: RE: [Histonet] Section position on slides > To: , > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > I learned from an old school HT and studied using the Carson book. In > this book there are several pages on where to put sections. If you > don't have the Frieda Carson book (or "the bible" as I refer to it), get > it. A new edition just came out about a year ago. If you're an ASCP > member I think the price is reduced... > For the most part, just put everything as close to the middle of the > clear glass part of the slide as possible. This also does well for IHC > automation. I think most automated machines you can adjust the drop > range too if you want to put the sections on the bottom or top? > Good Luck! > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya > Ewing-Finchem > Sent: Tuesday, February 15, 2011 11:09 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Section position on slides > > > I am trying to put together a training document around microtomy and > sectioning and am finding it hard to find information around the > placement of the actual sections on the slides. These are the > objectives I am looking to answer. Is this information found in any > publications? > > 1) Tissue / Section Placement: Are there published guidelines / > documentation on precisely where you should place tissue sections on a > 25mm x 75mm glass slide? Perhaps more importantly, where you should > NOT place tissue (ie. "x" mm from the edge of the glass slide)? > > 2) Diagnosable Slide Staining Area: With automation becoming more > widely used in IHC, are there published guidelines / documentation on > the usable or diagnosable staining area on a 25mm x 75mm glass slide? > For instance, would you define that as the area under a traditional > coverslip? Would this be defined as the entire slide below the label? > Or is this some distance from all the edges of the slide? With some > automated systems, it is near impossible to get edge to edge staining. > Is this acceptable? > > > Thanks for any ideas. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 13 > Date: Wed, 16 Feb 2011 10:15:29 -0500 > From: "Gill, Caula A." > Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis > To: "Ingles Claire " , > > Message-ID: > < > 087A9911BBAFDE4B8151CB148586E2C23A9F38@MDGEN-EXCH1.marylandgeneral.org> > > Content-Type: text/plain; charset="us-ascii" > > I agree, gum sounds better not to mention the taste > CG > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Tuesday, February 15, 2011 5:55 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis > > Wouldn't GUM be a more palatable option? Who knows where some of those > rubber bands have been! > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robert > Richmond > Sent: Mon 2/14/2011 11:09 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: saliva for glycogen hydrolysis > > > > Here's a truly hi-tech suggestion for doing PAS-diastase stains by the > spit method (which by the way is still how it's done by the small > pathology services I work on): > > You can produce copious quantities of saliva by the simple expedient of > chewing on a rubber band for a couple of minutes. This used to be the > technique used to obtain saliva samples for determination of ABH > substance secretor status in the blood bank. (I belong to that elite 20% > of donors who are non-secretors, and Lewis-a positive to prove > it.) > > Bob Richmond > Samurai pathologist and occasional sialogogue Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 14 > Date: Wed, 16 Feb 2011 10:25:27 -0500 > From: Victoria Baker > Subject: Re: [Histonet] Wage question for Wisconsin > To: Michael Hillmer > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Michael, > > ASCP has a wage/salary survey that they distribute based upon region and > certification. Some have said that this is biased as not everyone does > participate. I tend to agree with the survey, but also recognize that > dermatopathology is a 'specialty' type lab. I worked in Dermpath at NYU > back in the 80's to early 90's and our salaries were not identical to the > clinical labs as we were a part of the medical school and not the hospital. > Yes the hospital was 1199 at that time, I cannot say if they are still. > > My recommendation is to go with what you can find from the survey, but also > network with other dermpath or other specialty labs in your area that you > may know or who you can connect with through others - either on the > listserver or off - to possibly get a better sounding of salaries. HR at > your hospital might be also able to help if you have someone there that you > can work with and trust to obtain some of the data you're looking for and > understands what your specific needs are within the lab. I would include > your pathologists in this as much as possible as they will be needed to > support your request(s) as they are essentially your clients. > > Vikki > > On Tue, Feb 15, 2011 at 6:02 PM, Michael Hillmer < > mhillmer@dermwisconsin.com > > wrote: > > > We are dermatology clinic in Northeast Wisconsin and we are trying to do > > gather accurate wage data. Can anybody offer wages for an HT, HtL and > > lab assistants? Any help would be greatly appreciated. > > > > > > > > > > > > > > > > > > > > Thank you- > > > > > > > > Michael Hillmer PHR > > > > HR Coordinator > > > > Dermatology Associates of Wisconsin > > > > Phone: (920)683-5278 > > > > Fax: (920)686-9674 > > > > Cell: (920)860-6360 > > > > > > > > The materials and information in this e-mail are confidential and may > > contain Protected Health Information covered under the HIPAA Privacy > > Rule. If you are not the intended recipient, be advised that any > > unauthorized use, disclosure, copying, distribution, or action taken in > > reliance on the contents of this information is strictly forbidden by > > law. If you have received this e-mail in error, please notify me by > > reply e-mail and then delete this message. Do not pass any of this > > information to anyone else. Thank you for your cooperation. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 15 > Date: Wed, 16 Feb 2011 10:27:55 -0500 > From: "Shirley A. Powell" > Subject: [Histonet] Correction, Correction on cutoff date for GSH > meeting > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <9BF995BC0E47744E9673A41486E24EE22DBB3A7DE3@MERCERMAIL.MercerU.local > > > Content-Type: text/plain; charset="us-ascii" > > Correction: There is a correction on the cutoff date for the hotel > reservations. Cutoff date for discounted room rate is February 23rd, Not > March 1st. There are several other events going on that weekend and the > hotel is filling up fast. Please make your reservations and register soon > so you will not miss out on the great room rate and a great meeting. > > Shirley Powell > > > Hi Georgia, Alabama, ALL histotechs, > > > > The Georgia Society for Histotechnology invites you to our meeting March > 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near > Columbus, Ga. and very convenient to Alabama folks, so come across the line. > The invitation extends to any other states as well. Callaway Gardens is a > fantastic site for family vacations, golf lovers, nature lovers, so come to > Georgia for a visit and take in a wealth of histology knowledge. > > > > The deadline for making hotel reservations is March 1, 2011 so that gives > you a month to make your plans to attend, don't delay. The Mountain Creek > Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can > call for hotel reservations at 1-800-225-5292. Room rates start at $99 > which includes Continental Breakfast and Admission to the Park. For more > information about things to do at Callaway click on the link here: > http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx > > > > Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The > complete program can be downloaded from our website at this link: > www.histosearch.com/gsh http://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh>> then > click on GSH symposium link at the bottom of the home page. There you will > find the complete program with registration form. The vendor registration > form is on the same page for any last minute vendors who want to exhibit at > our meeting. If anyone has questions, please contact me for assistance. > > > > Come TRANSFORM yourselves. > > > > > > Shirley Powell > > GSH Secretary > > > Shirley A. Powell, HT(ASCP)HTL, QIHC > Technical Director > Histology Curricular Support Laboratory > Mercer University School of Medicine > 1550 College Street > Macon, GA 31207 > 478-301-2374 Lab > 478-301-5489 Fax > > > > ------------------------------ > > Message: 16 > Date: Wed, 16 Feb 2011 08:59:36 -0700 > From: "Breeden, Sara" > Subject: [Histonet] Pathco double-blade handle > To: "Histonet" > Message-ID: > < > 4D14F0FC9316DD41972D5F03C070908B02E4766A@nmdamailsvr.nmda.ad.nmsu.edu> > > Content-Type: text/plain; charset="us-ascii" > > Am looking for this item to replace one that was older than I am. I > need the handle (perhaps Pathco #D2877) for the blades (Pathco > #D2878-2C). The catch is that I want the SOLID BRASS handle - not the > aluminum. I've "googled" all over the place - can anyone help me find > these handles? Muchas gracias! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > > > ------------------------------ > > Message: 17 > Date: Wed, 16 Feb 2011 11:09:31 -0500 > From: "jstaruk" > Subject: RE: [Histonet] Pathco double-blade handle > To: "'Breeden, Sara'" , "'Histonet'" > > Message-ID: <005501cbcdf3$e623bd40$b26b37c0$@masshistology.com> > Content-Type: text/plain; charset="us-ascii" > > I've found two brass handles on Ebay in the last few years. I don't know > if > you're aware of this but the exact same blades that fit these handles can > be > purchased at Home Depot in the carpet section. $10 for 50 of them. > > > > Jim > > > > _______________________ > > James E. Staruk HT(ASCP) > > www.masshistology.com > > www.nehorselabs.com > > > > > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, > Sara > Sent: Wednesday, February 16, 2011 11:00 AM > To: Histonet > Subject: [Histonet] Pathco double-blade handle > > > > Am looking for this item to replace one that was older than I am. I > need the handle (perhaps Pathco #D2877) for the blades (Pathco > #D2878-2C). The catch is that I want the SOLID BRASS handle - not the > aluminum. I've "googled" all over the place - can anyone help me find > these handles? Muchas gracias! > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _____ > > No virus found in this message. > Checked by AVG - www.avg.com > Version: 10.0.1204 / Virus Database: 1435/3447 - Release Date: 02/16/11 > > > > ------------------------------ > > Message: 18 > Date: Wed, 16 Feb 2011 11:12:00 -0500 > From: "caymanfleck@gmail.com" > Subject: [Histonet] Exam Prep > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello Histoland, > > I'm preparing for the HTL exam after being trained on-the-job and working > in > histo for a few years. I'm using the standard 'Theory and practice...' > text > as my main resource. I'm wondering if anyone has used either the ASCP > practice tests (a set of 5 are available from ascp for about $30) or the > NSH > Self-assessment Program (which has modules that correspond to the exam > sections). The NSH program is expensive at about $240 but I don't know if > it's worth it. Has anyone used either of these resources? > > Thanks! > > > ------------------------------ > > Message: 19 > Date: Wed, 16 Feb 2011 10:23:52 -0600 > From: "Johnson, Nacaela" > Subject: RE: [Histonet] Exam Prep > To: , > Message-ID: > < > 6DBD71C31D7E444482E5D3DFBC202D260245D539@txhous1eb012.uson.usoncology.int> > > Content-Type: text/plain; charset="us-ascii" > > I just used the Freida Carson Histotechnology A Self-Instructional Test. > > > > Thanks, > > Nacaela Johnson, HTL (ASCP) > Histotechnologist > KCCC Pathology > 12000 110th St., Ste. 400 > Overland Park, KS 66210 > Office: 913-234-0576 > Fax: 913-433-7639 > Email: Nacaela.Johnson@USOncology.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > caymanfleck@gmail.com > Sent: Wednesday, February 16, 2011 10:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Exam Prep > > Hello Histoland, > > I'm preparing for the HTL exam after being trained on-the-job and > working in histo for a few years. I'm using the standard 'Theory and > practice...' text as my main resource. I'm wondering if anyone has used > either the ASCP practice tests (a set of 5 are available from ascp for > about $30) or the NSH Self-assessment Program (which has modules that > correspond to the exam sections). The NSH program is expensive at about > $240 but I don't know if it's worth it. Has anyone used either of these > resources? > > Thanks! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > The contents of this electronic mail message and any attachments are > confidential, possibly privileged and intended for the addressee(s) > only.
Only the addressee(s) may read, disseminate, retain or otherwise > use this message. If received in error, please immediately inform the sender > and then delete this message without disclosing its contents to > anyone. > > > > > ------------------------------ > > Message: 20 > Date: Wed, 16 Feb 2011 10:35:21 -0600 > From: > Subject: RE: [Histonet] Exam Prep > To: , , > > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > If you basically memorize this book, and then have a pretty good > understanding of IHC...then you should do fine. > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, > Nacaela > Sent: Wednesday, February 16, 2011 10:24 AM > To: caymanfleck@gmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Exam Prep > > I just used the Freida Carson Histotechnology A Self-Instructional Test. > > > > Thanks, > > Nacaela Johnson, HTL (ASCP) > Histotechnologist > KCCC Pathology > 12000 110th St., Ste. 400 > Overland Park, KS 66210 > Office: 913-234-0576 > Fax: 913-433-7639 > Email: Nacaela.Johnson@USOncology.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > caymanfleck@gmail.com > Sent: Wednesday, February 16, 2011 10:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Exam Prep > > Hello Histoland, > > I'm preparing for the HTL exam after being trained on-the-job and > working in histo for a few years. I'm using the standard 'Theory and > practice...' text as my main resource. I'm wondering if anyone has used > either the ASCP practice tests (a set of 5 are available from ascp for > about $30) or the NSH Self-assessment Program (which has modules that > correspond to the exam sections). The NSH program is expensive at about > $240 but I don't know if it's worth it. Has anyone used either of these > resources? > > Thanks! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > The contents of this electronic mail message and any attachments > are confidential, possibly privileged and intended for the addressee(s) > only.
Only the addressee(s) may read, disseminate, retain or > otherwise use this message. If received in error, please immediately > inform the sender and then delete this message without disclosing its > contents to anyone. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 21 > Date: Wed, 16 Feb 2011 11:08:07 -0600 > From: "Bill B." > Subject: Re: [Histonet] Section position on slides > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > As a pathologist I have some preferences on how certain tissues are > oriented on a slide and work with my histotechs so they can learn my prefs. > I also like my slides in certain orientation in the slide tray. EG, for > orientation of skin, I like the the dermis at the top when I am looking at > the slide. Some other tissues can be so oriented, some cannot. We often put > multiple levels on one slide and I would prefer them to be all oriented > similarly and in a straight line. > > The most annoying thing I see beyond coverslipping the wrong side, is the > label covering a part of the specimen. I don't care how close to the edge a > section is as long as it is all on the slide and covered by the cover-slip > or mounting medium. > > I have no idea is these preferences are weird. My point is the answer to > you 1st question may depend on your particular pathologists' preferences. > > BIll Blank > > At 10:09 PM -0700 2/15/11, Tanya Ewing-Finchem wrote: > >1) Tissue / Section Placement: Are there published guidelines / > documentation on precisely where you should place tissue sections on a 25mm > x 75mm glass slide? Perhaps more importantly, where you should NOT place > tissue (ie. ?x? mm from the edge of the glass slide)? > > > > > ------------------------------ > > Message: 22 > Date: Wed, 16 Feb 2011 17:20:57 +0000 > From: "Bartlett, Jeanine (CDC/OID/NCEZID)" > Subject: RE: [Histonet] Section position on slides > To: Tanya Ewing-Finchem , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I always try to center the section equally from sides and top-bottom as > possible. This means measure from the bottom of the frosted edge as the > "top". The Artisan special stains system has a clip that attaches around > the slide to allow reagents to pool onto the sections and incubate. If the > section is too close to the sides then these areas do not stain adequately. > Sometimes if the tissue section itself is very large this is unavoidable. > With automated coverslippers you must also consider placement of tissue to > allow for proper coverage. > > If I am cutting multiple unstained slides for subsequent testing I try to > orient the tissue the same on each slide to facilitate the reading of these > slides by the pathologist's > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya > Ewing-Finchem > Sent: Wednesday, February 16, 2011 12:09 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Section position on slides > > > I am trying to put together a training document around microtomy and > sectioning and am finding it hard to find information around the placement > of the actual sections on the slides. These are the objectives I am looking > to answer. Is this information found in any publications? > > 1) Tissue / Section Placement: Are there published guidelines / > documentation on precisely where you should place tissue sections on a 25mm > x 75mm glass slide? Perhaps more importantly, where you should NOT place > tissue (ie. "x" mm from the edge of the glass slide)? > > 2) Diagnosable Slide Staining Area: With automation becoming more widely > used in IHC, are there published guidelines / documentation on the usable or > diagnosable staining area on a 25mm x 75mm glass slide? For instance, would > you define that as the area under a traditional coverslip? Would this be > defined as the entire slide below the label? Or is this some distance from > all the edges of the slide? With some automated systems, it is near > impossible to get edge to edge staining. Is this acceptable? > > > > Thanks for any ideas. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 23 > Date: Wed, 16 Feb 2011 12:27:32 -0500 > From: Helen Fedor > Subject: RE: [Histonet] Section position on slides > To: "Bartlett, Jeanine (CDC/OID/NCEZID)" , Tanya > Ewing-Finchem , " > histonet@lists.utsouthwestern.edu" > > Message-ID: > < > DC2D3533BC05784793C2FCE01FCFB9260539A47E2B@JHEMTEXVS3.win.ad.jhu.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Since automation is becoming more and more a part of all Histology labs the > demands of placement of the tissue on the slides varies for different > instruments. Stainers, coverslippers and now with slide scanning as well. So > I do not believe that there is a silver bullet answer. > > Helen L. Fedor > > Tissue Microarray Lab, Manager > Prostate Spore Lab, Manager > Johns Hopkins University > 600 N. Wolfe St,?| Marburg Room 406 > Baltimore, MD?| 21287-7065 > > 410.614.1660 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine > (CDC/OID/NCEZID) > Sent: Wednesday, February 16, 2011 12:21 PM > To: Tanya Ewing-Finchem; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Section position on slides > > I always try to center the section equally from sides and top-bottom as > possible. This means measure from the bottom of the frosted edge as the > "top". The Artisan special stains system has a clip that attaches around > the slide to allow reagents to pool onto the sections and incubate. If the > section is too close to the sides then these areas do not stain adequately. > Sometimes if the tissue section itself is very large this is unavoidable. > With automated coverslippers you must also consider placement of tissue to > allow for proper coverage. > > If I am cutting multiple unstained slides for subsequent testing I try to > orient the tissue the same on each slide to facilitate the reading of these > slides by the pathologist's > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya > Ewing-Finchem > Sent: Wednesday, February 16, 2011 12:09 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Section position on slides > > > I am trying to put together a training document around microtomy and > sectioning and am finding it hard to find information around the placement > of the actual sections on the slides. These are the objectives I am looking > to answer. Is this information found in any publications? > > 1) Tissue / Section Placement: Are there published guidelines / > documentation on precisely where you should place tissue sections on a 25mm > x 75mm glass slide? Perhaps more importantly, where you should NOT place > tissue (ie. "x" mm from the edge of the glass slide)? > > 2) Diagnosable Slide Staining Area: With automation becoming more widely > used in IHC, are there published guidelines / documentation on the usable or > diagnosable staining area on a 25mm x 75mm glass slide? For instance, would > you define that as the area under a traditional coverslip? Would this be > defined as the entire slide below the label? Or is this some distance from > all the edges of the slide? With some automated systems, it is near > impossible to get edge to edge staining. Is this acceptable? > > > > > Thanks for any ideas. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 87, Issue 28 > **************************************** > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > > > ------------------------------ > > Message: 12 > Date: Thu, 17 Feb 2011 16:46:04 +0200 > From: "Gil Sharon" > Subject: [Histonet] Drosophila Sectioning > To: > Message-ID: <4BD4759CE4D84480B0D73E317744289B@danas> > Content-Type: text/plain; charset="us-ascii" > > Hi All, > > > > As a microbial ecologist, working with drosophila, I'm looking for a couple > of protocols (or papers) for fixation and paraffin-embedding of embryos, > 3rd > instar larvae, pupae and adult D. melanogaster (these sections will later > be > used for fluresecnt-in-situ-hybridization probing for specific bacteria). > Looking in the literature, I could only find protocols for embryos (or fly > heads). As I'm really new to histology, I hope someone here will be able to > help me with this. I have the help of a histo lab here, but they're working > on mice, so no experience with drosophila (or insects at all). > > > > Thanks in advance, > > Gil. > > > > > > ------------------------------ > > Message: 13 > Date: Thu, 17 Feb 2011 07:49:53 -0700 > From: "Patsy Ruegg" > Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP > section > To: "'Amy Lee'" , "'histonet'" > > Message-ID: <3A5E8FD47DF948B888D5F8765F834905@prueggihctechlt> > Content-Type: text/plain; charset="iso-8859-1" > > I do not know of a neutrophil specific ab for human ffpe tissue. You could > do just DAB without blocking with h202 for myleoperoxidase but it will not > distinguish between eosinophils and neutrophils myeloperoxidase will stain > all myeloid cells with granules in the cytoplasm (granulocytes). > > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee > Sent: Wednesday, February 16, 2011 1:07 PM > To: histonet > Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section > > Hello, > I was asked to perform neutrophil IHC on rat FFP sections. I searched > around > and found most are for mouse tissue. There is one from Serotec mouse anti > rat but it does not work on paraffin section. > > Could anybody recommend a good antibody? > > Thanks in advance, > > Amy > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 87, Issue 30 > **************************************** > -- Damien Laudier Laudier Histology (917) 836-7573 www.Laudierhistology.com From Margaret.Perry <@t> sdstate.edu Thu Feb 17 13:07:52 2011 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Feb 17 13:07:58 2011 Subject: [Histonet] liver endothelial stains Message-ID: What antibody would you recommend using to label the liver endothelial cells? Is factor 8 our best marker? Also do you know of a special stain other than H&E that would demonstrate these cells? Thank you. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From shive003 <@t> umn.edu Thu Feb 17 13:12:01 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Feb 17 13:12:08 2011 Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section References: <544851.36245.qm@web38005.mail.mud.yahoo.com> <3A5E8FD47DF948B888D5F8765F834905@prueggihctechlt> <4118E59E011945E589BB6D9076C9BAD3@Patsyoffice> Message-ID: The website says it works on humans. I have not tried this particular Ab myself, though I have tried another xNeutrophil Elastase. Jan Shivers ----- Original Message ----- From: "Patsy Ruegg" To: "'Jan Shivers'" ; "'Amy Lee'" ; "'histonet'" Sent: Thursday, February 17, 2011 12:20 PM Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section > Does it also work on human? > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > This email is confidential and intended solely for the use of the > Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. > Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. > If > you are NOT the intended recipient please contact the sender and dispose > of > this e-mail as soon as possible. > > -----Original Message----- > From: Jan Shivers [mailto:shive003@umn.edu] > Sent: Thursday, February 17, 2011 9:39 AM > To: Patsy Ruegg; 'Amy Lee'; 'histonet' > Subject: Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section > > Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE > tissues, > according to their website. > > Jan Shivers > > ----- Original Message ----- > From: "Patsy Ruegg" > To: "'Amy Lee'" ; "'histonet'" > > Sent: Thursday, February 17, 2011 8:49 AM > Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section > > > I do not know of a neutrophil specific ab for human ffpe tissue. You > could > do just DAB without blocking with h202 for myleoperoxidase but it will not > distinguish between eosinophils and neutrophils myeloperoxidase will stain > all myeloid cells with granules in the cytoplasm (granulocytes). > > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee > Sent: Wednesday, February 16, 2011 1:07 PM > To: histonet > Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section > > Hello, > I was asked to perform neutrophil IHC on rat FFP sections. I searched > around > and found most are for mouse tissue. There is one from Serotec mouse anti > rat but it does not work on paraffin section. > > Could anybody recommend a good antibody? > > Thanks in advance, > > Amy > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Valerie.Hannen <@t> parrishmed.com Thu Feb 17 14:34:54 2011 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Thu Feb 17 14:36:29 2011 Subject: .._RE: [Histonet] Zinc Formalin and IHC References: <4D5CECD9020000B9000F4C26@CVM.TAMU.EDU> Message-ID: <5680DA93771F0C48954CC8D38425E72401AD03E8@ISMAIL.parrishmed.local> We have used Buffered Zinc Formalin for many years and have not had any real problems with IHC or Special Stains. Valerie Hannen Parrish Medical Center Titusville, Florida ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lin Bustamante Sent: Thu 2/17/2011 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Zinc Formalin and IHC Posting for a friend. Replay to shernandez@st-joseph.org Is there any negative effect on IHC and Special Stain with the use of buffered Zinc Formalin? Thank you. Susan Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From Jason.PALMER <@t> svhm.org.au Thu Feb 17 16:33:19 2011 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Thu Feb 17 16:38:13 2011 Subject: [Histonet] RE: neutrophil Ab for rat FFPE Message-ID: Amy, We have used Abcam rabbit anti human myeloperoxidase (Ab9535) with very good results on FFPE rat tissue. No retrieval required, primary at 1:50, biotinylated secondary and ABC elite with DAB for detection. Hope that helps. Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Message: 7 Date: Wed, 16 Feb 2011 12:06:41 -0800 (PST) From: Amy Lee Subject: [Histonet] neutrophil antibody on rat FFP section To: histonet Message-ID: <544851.36245.qm@web38005.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. ? Could anybody recommend a good antibody? ? Thanks in advance, ? Amy Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From jnocito <@t> satx.rr.com Thu Feb 17 19:55:53 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Feb 17 19:56:22 2011 Subject: [Histonet] Tissue Processors Message-ID: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe From STACEY.LANGENBERG <@t> UCDENVER.EDU Thu Feb 17 20:33:13 2011 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Thu Feb 17 20:34:27 2011 Subject: [Histonet] Tissue Processors In-Reply-To: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> References: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> Message-ID: <1F70FCBB6D4EC549B2ADF69B9F9EAC034E6B03F404@STEAMBOAT.ucdenver.pvt> Joe, We are really impressed with the Leica ASP 300. We have a second one on the way. Stacey "People are not an interruption of our business. People are our business." Stacey Langenberg HT (ASCP) QIHC Laboratory Manager Histology/IF CU Dermatopathology Consultants 1999 N. Fitzsimons Pkwy Suite 120 Aurora, CO 80045 Lab-720-859-3559 Fax- 303-344-0789 Office- 303-577-2303 Cell-970-405-7742 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito [jnocito@satx.rr.com] Sent: Thursday, February 17, 2011 6:55 PM To: Histonet Subject: [Histonet] Tissue Processors Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From neil.fournier <@t> yale.edu Thu Feb 17 23:53:30 2011 From: neil.fournier <@t> yale.edu (Neil M. Fournier) Date: Thu Feb 17 23:53:35 2011 Subject: [Histonet] IHC pretreatment with NaOH/H2O2 Message-ID: <20110218005330.8h3p3wjcu84gc484@www.mail.yale.edu> I recently came across a protocol that was conducting IHC staining for pSTAT3 on free-floating fixed rat brain sections. The protocol stated that the tissue was placed in 1% NaOH and 1% H2O2 in H2O for 20 min, 0.3% glycine for 10 min, and 0.03% sodium dodecyl sulfate for 10 min. Could someone please explain to me the rationale for using NaOH and H2O2 pretreatment, as well as the additional steps in this procedure? My rationale may be incorrect but I would be concerned that the combination of NaOH and H2O2 might produce a too much O2 bubbling that could harm the tissue. I believe glycine is typically used for reduce autofluoresence since it binds to free aldehydes but this protocol was utilizing a cobolt/nickel enhanced-DAB reaction and I am unaware of any empirical study that has shown that glycine pretreatment improves peroxidase immunostaining. Finally, SDS seems like a fairly harsh detergent (although the remaining protocol utilizes Triton X-100 in blocking and antibody incubation steps), has anyone used this? and what would be the benefit of this over other detergents. Thanks in advance Neil From KellerP <@t> ent.wustl.edu Fri Feb 18 06:45:58 2011 From: KellerP <@t> ent.wustl.edu (Keller, Pat) Date: Fri Feb 18 06:46:04 2011 Subject: [Histonet] Eosinophilic new bone formation Message-ID: We have always noticed, at least in the middle/inner ear, that newly deposited bone stains darker than mature bone, both with H&E and toluidine blue. Is this increased eosinophilic quality due to a lack of mineralization and therefore higher density of osteoid components in the new bone or some other difference in composition of the osteoid? The contrast is quite striking when we observe bone remodeling due to middle ear infections, so I wanted to be able to offer an accurate explanation of why that is... Patricia Keller Sr. Research Tech/Core Histologist Washington University School of Medicine Department of Otolaryngology 4566 Scott Ave Campus Box 8115 St. Louis, Mo 63110 314-747-7166 This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. From rjbuesa <@t> yahoo.com Fri Feb 18 07:33:27 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 18 07:33:31 2011 Subject: [Histonet] Tissue Processors In-Reply-To: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> Message-ID: <366928.38959.qm@web65711.mail.ac4.yahoo.com> Sahura is still the best buy (for me). Ren? J. --- On Thu, 2/17/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] Tissue Processors To: "Histonet" Date: Thursday, February 17, 2011, 8:55 PM Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Fri Feb 18 07:59:44 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Feb 18 08:00:03 2011 Subject: [Histonet] Tissue Processors In-Reply-To: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> References: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> Message-ID: Joe, I just purchased a VIP6 because of my experience with this brand both while in the military and civilian life. Have never had a problem with them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, February 17, 2011 7:56 PM To: Histonet Subject: [Histonet] Tissue Processors Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From Ronald.Houston <@t> nationwidechildrens.org Fri Feb 18 08:16:37 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Feb 18 08:16:44 2011 Subject: [Histonet] Tissue Processors In-Reply-To: <366928.38959.qm@web65711.mail.ac4.yahoo.com> References: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> <366928.38959.qm@web65711.mail.ac4.yahoo.com> Message-ID: I would probably have gone along with you on this Rene but since we demo'ed, and subsequently purchased, the Peloris my opinion has changed Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 18, 2011 8:33 AM To: Histonet; Joe Nocito Subject: Re: [Histonet] Tissue Processors Sahura is still the best buy (for me). Ren? J. --- On Thu, 2/17/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] Tissue Processors To: "Histonet" Date: Thursday, February 17, 2011, 8:55 PM Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From ratliffjack <@t> hotmail.com Fri Feb 18 08:37:36 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Feb 18 08:37:51 2011 Subject: [Histonet] Eosinophilic new bone formation In-Reply-To: References: Message-ID: In decalcified sections of bone, yes the osteoid or dense unmineralized collagen matrix (mostly type I) will stain darker than mature native demineralized bone. Even though the mature bone has been demineralized, it is still more densely compact as compared to the newly formed bone that has not begun mineralization and . In resin embedded undemineralized sections of bone, the contrast is exactly opposite when staining with Von Kossa and counterstaining with MacNeal's tetrachrome and similar to decalcified sections in a Goldner's trichrome where the acid fuchsin stains osteoid darker than the light green does the mineralized bone. So the answer is yes, tissue density is what plays the major role in contrast staining and stain intensity. Jack On Feb 18, 2011, at 6:45 AM, "Keller, Pat" wrote: > We have always noticed, at least in the middle/inner ear, that newly > deposited bone stains darker than mature bone, both with H&E and > toluidine blue. Is this increased eosinophilic quality due to a lack of > mineralization and therefore higher density of osteoid components in the > new bone or some other difference in composition of the osteoid? The > contrast is quite striking when we observe bone remodeling due to middle > ear infections, so I wanted to be able to offer an accurate explanation > of why that is... > > > > > > Patricia Keller > Sr. Research Tech/Core Histologist > Washington University School of Medicine > Department of Otolaryngology > 4566 Scott Ave > Campus Box 8115 > St. Louis, Mo 63110 > 314-747-7166 > > > > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brian <@t> prometheushealthcare.com Fri Feb 18 09:33:29 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Fri Feb 18 09:33:41 2011 Subject: [Histonet] Histotechnologist with a FL HTL license for Fort Myers, FL Message-ID: <008101cbcf81$344c5660$9ce50320$@com> Looking for a Histotechnologist with a FL HTL license for Fort Myers, FL. This will be a late second or third shift. Experience with IHC and Ventanna is preferred. Please contact me today for immediate consideration. Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From sgoebel <@t> mirnarx.com Fri Feb 18 10:00:18 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Feb 18 10:00:22 2011 Subject: [Histonet] Tissue Processors In-Reply-To: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> References: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> Message-ID: Pathcenters in my opinion don't last as long? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, February 17, 2011 7:56 PM To: Histonet Subject: [Histonet] Tissue Processors Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Fri Feb 18 10:47:56 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Feb 18 10:47:59 2011 Subject: [Histonet] Microwave Usage, Monitoring procedure Message-ID: <1872B4A455B7974391609AD8034C79FC026DFD69@LBEXCH01.hchd.local> Hello to all in histoland. I am in need of a procedure for the usgae, monitoring and container venting of the mcrowave. I have a procedure that I wrote several years ago, but my boss wants it updated. Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From dgoodwin <@t> rwjuhh.edu Fri Feb 18 12:45:36 2011 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Fri Feb 18 12:45:42 2011 Subject: [Histonet] RE: Histonet Digest, Vol 87, Issue 33 In-Reply-To: <1298052298.822776@messagescreen2.rwjham.net> References: <1298052298.822776@messagescreen2.rwjham.net> Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71D1D056269@HAMEXMBA.rwjham.local> We are also fans of the Leica ASP 300 processor. Diana G. Goodwin, BS, HT(ASCP)QIHC Department of Pathology Robert Wood Johnson University Hospital at Hamilton Hamilton, NJ 08610 609-631-6996 dgoodwin@rwjuhh.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, February 18, 2011 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Tissue Processors (Joe Nocito) 2. RE: Tissue Processors (Langenberg, Stacey) 3. IHC pretreatment with NaOH/H2O2 (Neil M. Fournier) 4. Eosinophilic new bone formation (Keller, Pat) 5. Re: Tissue Processors (Rene J Buesa) 6. RE: Tissue Processors (Nails, Felton) 7. RE: Tissue Processors (Houston, Ronald) 8. Re: Eosinophilic new bone formation (Jack Ratliff) 9. Histotechnologist with a FL HTL license for Fort Myers, FL (Brian- Prometheus) 10. RE: Tissue Processors (sgoebel@mirnarx.com) 11. Microwave Usage, Monitoring procedure (Scott, Allison D) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Feb 2011 19:55:53 -0600 From: "Joe Nocito" Subject: [Histonet] Tissue Processors To: "Histonet" Message-ID: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC> Content-Type: text/plain; charset="iso-8859-1" Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe ------------------------------ Message: 2 Date: Thu, 17 Feb 2011 19:33:13 -0700 From: "Langenberg, Stacey" Subject: RE: [Histonet] Tissue Processors To: Joe Nocito , Histonet Message-ID: <1F70FCBB6D4EC549B2ADF69B9F9EAC034E6B03F404@STEAMBOAT.ucdenver.pvt> Content-Type: text/plain; charset="us-ascii" Joe, We are really impressed with the Leica ASP 300. We have a second one on the way. Stacey "People are not an interruption of our business. People are our business." Stacey Langenberg HT (ASCP) QIHC Laboratory Manager Histology/IF CU Dermatopathology Consultants 1999 N. Fitzsimons Pkwy Suite 120 Aurora, CO 80045 Lab-720-859-3559 Fax- 303-344-0789 Office- 303-577-2303 Cell-970-405-7742 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito [jnocito@satx.rr.com] Sent: Thursday, February 17, 2011 6:55 PM To: Histonet Subject: [Histonet] Tissue Processors Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 18 Feb 2011 00:53:30 -0500 From: "Neil M. Fournier" Subject: [Histonet] IHC pretreatment with NaOH/H2O2 To: histonet@lists.utsouthwestern.edu Message-ID: <20110218005330.8h3p3wjcu84gc484@www.mail.yale.edu> Content-Type: text/plain; charset=ISO-8859-1 I recently came across a protocol that was conducting IHC staining for pSTAT3 on free-floating fixed rat brain sections. The protocol stated that the tissue was placed in 1% NaOH and 1% H2O2 in H2O for 20 min, 0.3% glycine for 10 min, and 0.03% sodium dodecyl sulfate for 10 min. Could someone please explain to me the rationale for using NaOH and H2O2 pretreatment, as well as the additional steps in this procedure? My rationale may be incorrect but I would be concerned that the combination of NaOH and H2O2 might produce a too much O2 bubbling that could harm the tissue. I believe glycine is typically used for reduce autofluoresence since it binds to free aldehydes but this protocol was utilizing a cobolt/nickel enhanced-DAB reaction and I am unaware of any empirical study that has shown that glycine pretreatment improves peroxidase immunostaining. Finally, SDS seems like a fairly harsh detergent (although the remaining protocol utilizes Triton X-100 in blocking and antibody incubation steps), has anyone used this? and what would be the benefit of this over other detergents. Thanks in advance Neil ------------------------------ Message: 4 Date: Fri, 18 Feb 2011 06:45:58 -0600 From: "Keller, Pat" Subject: [Histonet] Eosinophilic new bone formation To: Message-ID: Content-Type: text/plain; charset="us-ascii" We have always noticed, at least in the middle/inner ear, that newly deposited bone stains darker than mature bone, both with H&E and toluidine blue. Is this increased eosinophilic quality due to a lack of mineralization and therefore higher density of osteoid components in the new bone or some other difference in composition of the osteoid? The contrast is quite striking when we observe bone remodeling due to middle ear infections, so I wanted to be able to offer an accurate explanation of why that is... Patricia Keller Sr. Research Tech/Core Histologist Washington University School of Medicine Department of Otolaryngology 4566 Scott Ave Campus Box 8115 St. Louis, Mo 63110 314-747-7166 This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. ------------------------------ Message: 5 Date: Fri, 18 Feb 2011 05:33:27 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Tissue Processors To: Histonet , Joe Nocito Message-ID: <366928.38959.qm@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Sahura is still the best buy (for me). Ren? J. --- On Thu, 2/17/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] Tissue Processors To: "Histonet" Date: Thursday, February 17, 2011, 8:55 PM Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 18 Feb 2011 07:59:44 -0600 From: "Nails, Felton" Subject: RE: [Histonet] Tissue Processors To: "'Joe Nocito'" , "Histonet" Message-ID: Content-Type: text/plain; charset=us-ascii Joe, I just purchased a VIP6 because of my experience with this brand both while in the military and civilian life. Have never had a problem with them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, February 17, 2011 7:56 PM To: Histonet Subject: [Histonet] Tissue Processors Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== ------------------------------ Message: 7 Date: Fri, 18 Feb 2011 09:16:37 -0500 From: "Houston, Ronald" Subject: RE: [Histonet] Tissue Processors To: 'Rene J Buesa' , Histonet , Joe Nocito Message-ID: Content-Type: text/plain; charset="iso-8859-1" I would probably have gone along with you on this Rene but since we demo'ed, and subsequently purchased, the Peloris my opinion has changed Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 18, 2011 8:33 AM To: Histonet; Joe Nocito Subject: Re: [Histonet] Tissue Processors Sahura is still the best buy (for me). Ren? J. --- On Thu, 2/17/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] Tissue Processors To: "Histonet" Date: Thursday, February 17, 2011, 8:55 PM Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 8 Date: Fri, 18 Feb 2011 08:37:36 -0600 From: Jack Ratliff Subject: Re: [Histonet] Eosinophilic new bone formation To: "Keller, Pat" Cc: "" Message-ID: Content-Type: text/plain; charset="us-ascii" In decalcified sections of bone, yes the osteoid or dense unmineralized collagen matrix (mostly type I) will stain darker than mature native demineralized bone. Even though the mature bone has been demineralized, it is still more densely compact as compared to the newly formed bone that has not begun mineralization and . In resin embedded undemineralized sections of bone, the contrast is exactly opposite when staining with Von Kossa and counterstaining with MacNeal's tetrachrome and similar to decalcified sections in a Goldner's trichrome where the acid fuchsin stains osteoid darker than the light green does the mineralized bone. So the answer is yes, tissue density is what plays the major role in contrast staining and stain intensity. Jack On Feb 18, 2011, at 6:45 AM, "Keller, Pat" wrote: > We have always noticed, at least in the middle/inner ear, that newly > deposited bone stains darker than mature bone, both with H&E and > toluidine blue. Is this increased eosinophilic quality due to a lack of > mineralization and therefore higher density of osteoid components in the > new bone or some other difference in composition of the osteoid? The > contrast is quite striking when we observe bone remodeling due to middle > ear infections, so I wanted to be able to offer an accurate explanation > of why that is... > > > > > > Patricia Keller > Sr. Research Tech/Core Histologist > Washington University School of Medicine > Department of Otolaryngology > 4566 Scott Ave > Campus Box 8115 > St. Louis, Mo 63110 > 314-747-7166 > > > > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Fri, 18 Feb 2011 10:33:29 -0500 From: "Brian- Prometheus" Subject: [Histonet] Histotechnologist with a FL HTL license for Fort Myers, FL To: Message-ID: <008101cbcf81$344c5660$9ce50320$@com> Content-Type: text/plain; charset="us-ascii" Looking for a Histotechnologist with a FL HTL license for Fort Myers, FL. This will be a late second or third shift. Experience with IHC and Ventanna is preferred. Please contact me today for immediate consideration. Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog ------------------------------ Message: 10 Date: Fri, 18 Feb 2011 10:00:18 -0600 From: Subject: RE: [Histonet] Tissue Processors To: , Message-ID: Content-Type: text/plain; charset="US-ASCII" Pathcenters in my opinion don't last as long? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, February 17, 2011 7:56 PM To: Histonet Subject: [Histonet] Tissue Processors Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 18 Feb 2011 10:47:56 -0600 From: "Scott, Allison D" Subject: [Histonet] Microwave Usage, Monitoring procedure To: Message-ID: <1872B4A455B7974391609AD8034C79FC026DFD69@LBEXCH01.hchd.local> Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. I am in need of a procedure for the usgae, monitoring and container venting of the mcrowave. I have a procedure that I wrote several years ago, but my boss wants it updated. Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 87, Issue 33 **************************************** From TJJ <@t> stowers.org Fri Feb 18 13:07:13 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Feb 18 13:07:18 2011 Subject: [Histonet] Re: IHC pretreatment with NaOH/H2O2 Message-ID: Dear Neil, I was able to find a protocol like you described published where they had done a regular IHC protocol on free-floating tissue sections for a-MSH antibody using 0.3% H2O2, normal serum block, etc. They go on to describe that for P-STAT3, "the tissue needed to be pretreated with 1% NaOH and 1%H2O2 in water for 20 minutes, 0.3% glycine for 10 minutes, and 0.03% SDS for 10 minutes". After that it looks like a standard protocol. Here's the URL for that paper: http://endo.endojournals.org/cgi/reprint/144/5/2121?ijkey=23f435f1cf61629953465217ca752dd73df366e9 They don't say why this was necessary, but it might be worth trying to track down one of the authors and ask them. It seems like the sodium hydroxide and hydrogen peroxide serves as an oxidizer somehow. Since they are not doing IF, maybe the glycine is needed to bind with glycine receptors in the brain prior to staining? And I suspect the SDS is needed for permeabilization, even though they are using a sectioned sample. The right detergent can make all the difference. I would really be interested in knowing what the rationale is, but it seems like they figured out what physiologic changes needed to happen for this antibody to work in brain. If you do have a conversation with the authors, please report back here because my curiosity is now at high roar. Best wishes, Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From Montina.VanMeter <@t> pbrc.edu Fri Feb 18 13:50:26 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri Feb 18 13:50:30 2011 Subject: [Histonet] Re: IHC pretreatment with NaOH/H2O2 In-Reply-To: References: Message-ID: <4FE7FB862E90E448AE32388E759220E502ACE42C@pbrcas31.pbrc.edu> Neil, When I read the paper in question, it reminded me of the pretreatments one would use in an In Situ Hybridization protocol. I have used this protocol in the past and it doesn't seem to harm the tissue as long as the tissue has been adequately perfused. We use the pSTAT3 #9131 (Cell Signaling) with 40um free-floating rat brainstem sections that have been perfused with 4% paraformaldehyde . After successfully reproducing their protocol, I decided to try and incorporate my own protocol for this particular antibody. The only pretreatment that I use is a cocktail of Methanol (cold) + 1% H2O2 for 20 minutes., TBST Rinses, Blocking step - Rodent Block R, Primary - pSTAT3 (1:50 - 1:100) - overnight, TBST Rinses, Alexa Fluor secondary - 2hrs., TBST Rinses, mount & coverslip with ProLong Gold (Invitrogen). **The #9131 spec sheet notes that you must use Methanol as a pretreatment step for this anitbody. *** I am often required to use this antibody in double labeling experiments with tract tracing beads that are sensitive to heat. HIER is out of the question, but the methanol/H2O2 pretreatment appears to be adequate for this antibody and doesn't affect the beads. Regards, Tina Montina J. Van Meter Lab Manager Pennington Biomedical Research Center Autonomic Neuroscience -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Friday, February 18, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: IHC pretreatment with NaOH/H2O2 Dear Neil, I was able to find a protocol like you described published where they had done a regular IHC protocol on free-floating tissue sections for a-MSH antibody using 0.3% H2O2, normal serum block, etc. They go on to describe that for P-STAT3, "the tissue needed to be pretreated with 1% NaOH and 1%H2O2 in water for 20 minutes, 0.3% glycine for 10 minutes, and 0.03% SDS for 10 minutes". After that it looks like a standard protocol. Here's the URL for that paper: http://endo.endojournals.org/cgi/reprint/144/5/2121?ijkey=23f435f1cf6162 9953465217ca752dd73df366e9 They don't say why this was necessary, but it might be worth trying to track down one of the authors and ask them. It seems like the sodium hydroxide and hydrogen peroxide serves as an oxidizer somehow. Since they are not doing IF, maybe the glycine is needed to bind with glycine receptors in the brain prior to staining? And I suspect the SDS is needed for permeabilization, even though they are using a sectioned sample. The right detergent can make all the difference. I would really be interested in knowing what the rationale is, but it seems like they figured out what physiologic changes needed to happen for this antibody to work in brain. If you do have a conversation with the authors, please report back here because my curiosity is now at high roar. Best wishes, Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Fri Feb 18 14:07:44 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Feb 18 14:06:06 2011 Subject: [Histonet] Information Needed Message-ID: Hi, I need information for purchasing a good vacuum with proper biological filtration for cleaning a cryostat. We are not buying a new cryostat and I do know of several with a decontamination feature, it is not in the budget for the next two years. Thanks, Pamela A Marcum Anatomic Pathology Manager University of Arkansas for Medical Sciences 4301 W Markham St Slot 502 Little Rock AR 72205 Office: 501-686-5941 Pager: 501-395-1943 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From cls71877 <@t> sbcglobal.net Fri Feb 18 15:03:56 2011 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Fri Feb 18 15:03:58 2011 Subject: [Histonet] Job Descriptions Message-ID: <910213.42682.qm@web81208.mail.mud.yahoo.com> Hello Histoland, I was wondering if anyone out there may have their job decriptions for histologists, lab assistants, supervisor and/or medical director available and would be willing to share.? I am currently revamping mine and am struggling with some of the verbiage.? Thank you all and have a fantastic weekend! Cristi From kgrobert <@t> rci.rutgers.edu Fri Feb 18 15:38:02 2011 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Fri Feb 18 15:38:06 2011 Subject: [Histonet] LKB Bromma 7800 knifemaker Message-ID: <375198e49e84933b6323635edb4609dc.squirrel@webmail.rci.rutgers.edu> Yes, I know this is ancient. But does anyone know of a service company that can repair this machine? Ours works, sort of, but it's very frustrating. Thanks and have a good weekend, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 From mhillmer <@t> dermwisconsin.com Fri Feb 18 15:47:37 2011 From: mhillmer <@t> dermwisconsin.com (Michael Hillmer) Date: Fri Feb 18 15:47:42 2011 Subject: [Histonet] Job Opening Message-ID: <9060F1C2EB3D8A4AAF3B3433C9003230F9790F@hostexchbe1.hosting.tushausweb.com> We are looking for a certified HT or HTL to join our pathology lab in Manitowoc, WI. We have a currently 2nd shift position open. We have excellent benefits, state-of-the-are lab (less than 3 months old) and a great team to work with. We will assist with relocation. Please contact me if you have any questions. Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 Fax: (920)686-9674 Cell: (920)860-6360 The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. From liz <@t> premierlab.com Fri Feb 18 15:57:04 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Feb 18 15:57:08 2011 Subject: [Histonet] Information Needed In-Reply-To: Message-ID: Pamela We use a hepa filtered vacuum from Marmed, Inc. (440) 572-5175 LiZ Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Friday, February 18, 2011 1:08 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Information Needed Hi, I need information for purchasing a good vacuum with proper biological filtration for cleaning a cryostat. We are not buying a new cryostat and I do know of several with a decontamination feature, it is not in the budget for the next two years. Thanks, Pamela A Marcum Anatomic Pathology Manager University of Arkansas for Medical Sciences 4301 W Markham St Slot 502 Little Rock AR 72205 Office: 501-686-5941 Pager: 501-395-1943 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Sat Feb 19 11:58:41 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Sat Feb 19 11:58:45 2011 Subject: [Histonet] Histotech Needed Message-ID: <20110219175841.C951C2787@bellona.cnchost.com> Histotech needed for vacation coverages and Saturday coverage. We are an independent laboratory located in Fountain Valley, California. If interested, I will give you more details. Thank you, Paula Lucas Bio-Path Medical Group Fountain Valley, CA From liz <@t> premierlab.com Sat Feb 19 16:59:18 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Feb 19 16:59:22 2011 Subject: [Histonet] IgG and IgM on mouse kidneys Message-ID: Hello all I have a quick question. Can I perform IgG and IgM staining on mouse kidneys on FFPE tissue samples? I thought that this type of staining is normally completed on frozen sections via Immunofluorescence. Any advice would be helpful Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From jnocito <@t> satx.rr.com Sat Feb 19 18:04:27 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Feb 19 18:04:58 2011 Subject: [Histonet] Thank you Message-ID: <1F876798278147F69D332C7040BA791F@JoePC> Just wanted to thank you all for your advice, likes, dislikes and experiences. I am really honored to be part of this group. Seriously. It's refreshing. Joe From kdwyer3322 <@t> aol.com Sun Feb 20 10:42:08 2011 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Feb 20 10:42:32 2011 Subject: [Histonet] Texas Society for Histotechnology 2011 Symposium/Convention, Plano Texas Message-ID: <8CD9F343F162B07-1C20-4A53B@webmail-m085.sysops.aol.com> All, The TSH meeting will be held March 31-April 3, 2011. The meeting will start with a Vendor/Attendee Golf Outing. There will be Food, Fun and Prizes! Contact RebeccaRuser@mhd.com for registration or information. The meeting will begin Friday April 1, 2011 with 2 Workshops and the opening of the Vendor Exhibits. We have to date over 30 Vendors represented. Contact Sandra Christiansen at sandra.christiansen@christushealth.org for vendor information or to exhibit. It is not to late to sign up for a variety of interesting symposiums and workshops. Pre-registration deadline is March 25, 2011. There are still plenty of rooms at the Marriott Dallas/Plano at the Legacy Twon Center, 7120 Dallas Parkway, Plano Texas. Room rates are $109.00/night. Contact 972-473-6444 to book a room. Hotel deadline is March 10, 2011. If you would like a copy of the TSH program electronically please contact Kathy Dwyer via this e-mail or kdwyer3322@aol.com We hope to see you in TEXAS! Regards, TSH Convention Committee From etambutte <@t> centrescientifique.mc Sun Feb 20 12:09:05 2011 From: etambutte <@t> centrescientifique.mc (Eric Tambutte) Date: Sun Feb 20 12:09:11 2011 Subject: [Histonet] Re: Histonet Digest, Vol 87, Issue 35 Message-ID: <1045731618@s15272523.onlinehome-server.info> Merci pour votre message. Je suis actuellement absent du laboratoire. Je repondrai a votre message a mon retour a partir du 28 fevrier 2011. En cas d'urgence merci de contacter : Mme Corinne Gaziello (cgaziello@gouv.mc) et/ou Muriel Shilot (muriel@centrescientifique.mc). Thank you for your email. I am currently out of the lab. I will reply to your message after the 28th of february 2011. For any administrative problem, contact the Administrative Director of the CSM, Corinne Gaziello (cgaziello@gouv.mc) and Muriel Shilot (muriel@centrescientifique.mc). From Milton.Gomez <@t> nyumc.org Sun Feb 20 14:00:07 2011 From: Milton.Gomez <@t> nyumc.org (Gomez, Milton) Date: Sun Feb 20 14:00:11 2011 Subject: [Histonet] Pin4cocktail negative controls Message-ID: <4A53F9A1D7C2674FA4A6E650D703DDA5D9308A96@MSGWSDCPMB07.nyumc.org> Hello Histonetters, What are you folks using as a negative control when running Pin4 cocktail antibody. Thanks in advance, MG ------------------------------------------------------------
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From kimd <@t> slonepartners.com  Sun Feb 20 21:21:00 2011
From: kimd <@t> slonepartners.com (Kim Wilson)
Date: Sun Feb 20 21:21:05 2011
Subject: [Histonet] Histotechnologist wanted in DC metro area
Message-ID: <20110221032104.A54605331E@qmail2.topechelon.com>


   Slone  Partners seeks   laboratory, based in a   

   
ASCP   certification   experience in a high-volume laboratory.
   

   
Special  features  of  the  position:    curiosity and enjoys mentoring and developing   

   
Interested  and qualified candidates should   Kim Wilson at [1]kimd@slonepartners.com.

References

   1. 3D"mailto:kimd@slonepartners.com"
From cbrya <@t> lexclin.com  Mon Feb 21 08:07:42 2011
From: cbrya <@t> lexclin.com (Carol Bryant)
Date: Mon Feb 21 08:07:46 2011
Subject: [Histonet] RE: Pin4cocktail negative controls
In-Reply-To: <4A53F9A1D7C2674FA4A6E650D703DDA5D9308A96@MSGWSDCPMB07.nyumc.org>
References: <4A53F9A1D7C2674FA4A6E650D703DDA5D9308A96@MSGWSDCPMB07.nyumc.org>
Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BE623F46@EXCHANGESB>

I would like that information also. 
Thank you,
Carol 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton
Sent: Sunday, February 20, 2011 3:00 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Pin4cocktail negative controls

Hello Histonetters,

What are you folks using as a negative control when running Pin4 cocktail antibody.

Thanks in advance,
MG
------------------------------------------------------------
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================================= 
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


NOTICE OF CONFIDENTIALITY

This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.  If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.  Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. 


From chapcl <@t> yahoo.com  Mon Feb 21 08:16:25 2011
From: chapcl <@t> yahoo.com (William Chappell)
Date: Mon Feb 21 08:16:35 2011
Subject: [Histonet] RE: Pin4cocktail negative controls
In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BE623F46@EXCHANGESB>
References: <4A53F9A1D7C2674FA4A6E650D703DDA5D9308A96@MSGWSDCPMB07.nyumc.org>
	<50DA0C6B72976B4AB3A0FCA04CC73DBF05BE623F46@EXCHANGESB>
Message-ID: 

I assume you mean what reagent to use as the negative control?  The ideal control is a mixture of negative mouse serum and negative rabbit specifically titered to match the antibody concentration in the cocktail, which is probably proprietary. The next best solution is to use a universal negative control serum. The universal component typically denotes use with rabbit and mouse antibodies. 

Will Chappell, HTL(ASCP)QIHC
Histologist at large

Sent from my iPhone

On Feb 21, 2011, at 8:07 AM, Carol Bryant  wrote:

> I would like that information also. 
> Thank you,
> Carol 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton
> Sent: Sunday, February 20, 2011 3:00 PM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: [Histonet] Pin4cocktail negative controls
> 
> Hello Histonetters,
> 
> What are you folks using as a negative control when running Pin4 cocktail antibody.
> 
> Thanks in advance,
> MG
>
> > > ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
> ================================= > > > 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> NOTICE OF CONFIDENTIALITY
> 
> This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.  If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.  Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. 
> 
> 
> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From kmerriam2003 <@t> yahoo.com  Mon Feb 21 08:39:28 2011
From: kmerriam2003 <@t> yahoo.com (Kim Merriam)
Date: Mon Feb 21 08:39:32 2011
Subject: [Histonet] TRAP staining kit
Message-ID: <39735.94164.qm@web130120.mail.mud.yahoo.com>

Hi All,
?
Does anyone know if there is a TRAP staining kit available for histology (and 
who sells it)?? I have?found a bunch of?them on google, but?they are all for 
cell culture.
?
Thanks,
Kim
?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 


      
From jstaruk <@t> masshistology.com  Mon Feb 21 08:59:01 2011
From: jstaruk <@t> masshistology.com (jstaruk)
Date: Mon Feb 21 08:59:03 2011
Subject: [Histonet] TRAP staining kit
In-Reply-To: <39735.94164.qm@web130120.mail.mud.yahoo.com>
References: <39735.94164.qm@web130120.mail.mud.yahoo.com>
Message-ID: <00ca01cbd1d7$dfe56e00$9fb04a00$@masshistology.com>

Hi Kim,

 

I've successively used the Sigma kit #387A-1KT in the past

 

Jim

 

_______________________

James E. Staruk HT(ASCP)

   www.masshistology.com

   www.nehorselabs.com

 

 

From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Monday, February 21, 2011 9:39 AM
To: Histonet
Subject: [Histonet] TRAP staining kit

 

Hi All,
 
Does anyone know if there is a TRAP staining kit available for histology
(and
who sells it)?  I have found a bunch of them on google, but they are all for
cell culture.
 
Thanks,
Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA


     
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

  _____  

No virus found in this message.
Checked by AVG - www.avg.com
Version: 10.0.1204 / Virus Database: 1435/3457 - Release Date: 02/21/11

From marktarango <@t> gmail.com  Mon Feb 21 09:26:28 2011
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Mon Feb 21 09:26:33 2011
Subject: [Histonet] Pin4cocktail negative controls
In-Reply-To: <4A53F9A1D7C2674FA4A6E650D703DDA5D9308A96@MSGWSDCPMB07.nyumc.org>
References: <4A53F9A1D7C2674FA4A6E650D703DDA5D9308A96@MSGWSDCPMB07.nyumc.org>
Message-ID: 

Hi Milton,

We use Biocare's Universal Negative Control Serum in place of the antibodies
in the negative control staining protocol.  We use normal prostate tissue to
show lack of p504s staining (to make sure it isn't overstaining normal
glands).  The prostate cancer in the tissue control shows lack of p63+ and
CK34BE12+ basal cells.

I hope this helps.

Mark
On Sun, Feb 20, 2011 at 12:00 PM, Gomez, Milton wrote:

> Hello Histonetters,
>
> What are you folks using as a negative control when running Pin4 cocktail
> antibody.
>
> Thanks in advance,
> MG
>
> > > ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the > intended recipient(s) and may contain information that is proprietary, > confidential, and exempt from disclosure under applicable law. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > have received this email in error please notify the sender by return email > and delete the original message. Please note, the recipient should check > this email and any attachments for the presence of viruses. The organization > accepts no liability for any damage caused by any virus transmitted by this > email.
> ================================= > > > 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From NKonop <@t> chw.org  Mon Feb 21 09:50:31 2011
From: NKonop <@t> chw.org (Konop, Nicole)
Date: Mon Feb 21 09:50:37 2011
Subject: [Histonet] Pediatric Histotech Position, Milwaukee WI
Message-ID: 

Children's Hospital of Wisconsin, the area's premier provider of pediatric care, has a full-time opportunity available for a Histology Technician.  This is an opportunity to work in a state of the art pediatric, CAP accredited histology lab. The histologist will be responsible for performing procedures including frozen sections, embedding, microtomy, special stains, immunohistochemical stains and limited cytology procedures on specimens from patients of all ages including neonatal, infant, toddler, pre-school, school-age, adolescent, and selected adults.

Our Histology Lab uses current technology to offer quality care to the children we serve.  This technology includes Tissue-Tek embedding center, Microm automated microtomes, Leica  ST5020 stainer with attached glass coverlsipper, Peloris tissue processor, Dako Artisan special stainer and Leica Bond Max immunostaining platform.

This is a full time day shift position with rotating start times.  A Saturday rotation is required as well.


Candidates will have an Associate Degree in clinical laboratory sciences, one of the specialty areas, or one of the chemical or biological sciences.  Current ASCP HT or HTL certification or HT/HTL registry eligible is preferred. Six to twelve months prior experience is preferred.

For more information, visit chw.org/jobs or contact Megan Olson, Human Resources recruiter at mkolson@chw.org or (414) 337-7367.

Use this direct link to get to the job posting
https://extrlapp.chw.org/psc/extrlapp/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_JOB_DTL&Action=A&JobOpeningId=20094&SiteId=1&PostingSeq=1


Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department



From alyssa <@t> alliedsearchpartners.com  Mon Feb 21 10:32:51 2011
From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson)
Date: Mon Feb 21 10:33:05 2011
Subject: [Histonet] Histotech Needed on FL Gulf Coast
Message-ID: 

Position: Histology Technician, Histotechnician, Histotechnologist, Histologist

 Location: Panama City, FL



Schedule: Alternates between two shifts. Monday-Friday, 5am-1:30m, and
7am-3:30pm. Day Shift hours.



Description

 ?         Perform routine and complex histology procedures utilized
in pathology diagnosis.

?         Aid clinical practitioners in confirming or ruling out
diagnoses, monitoring chronic disease states, and analyzing the
effects of medical therapies.

 ESSENTIAL CORE FUNCTIONS:

?         Uses knowledge of laboratory science to perform the most
complex clinical testing as defined by the Clinical Laboratory
Improvement Amendments (CLIAA '88) providing safe and effective
patient care.

?         Maintains strict safety and infection control techniques at all times.

?         Practices, develops and endorses customer service skills in
relationships with internal and external customers.

?         Participate in Quality Improvement activities as assigned.

?         Actively seeks ways to control costs without compromising
patient safety, quality of care or the services delivered.

?         Follows established guidelines for reporting a significant
medical error or unanticipated outcome in the patient's care which
result in patient harm.

?         Uses occurrence reporting system to report potential patient
safety issues.

?         Demonstrates knowledge of Laboratory Information System.

?         Uses Performance Improvement Plan to improve patient safety.



Qualifications

 EDUCATION REQUIRED AND/OR PREFERRED:

- Vocational / Technical

- 2 year / Associate Degree

- 4 year/Bachelor's Degree

- License / Certification

 LICENSURE/CERTIFICATION:

- Florida Licensure as Clinical Laboratory Technologist / Supervisor /
Technician

 EXPERIENCE REQUIRED OR PREFERRED:

-1-3 years

 SKILLS, KNOWLEDGE, AND ABILITIES:

- Reads and writes Basic English possessing the basic skills necessary
to meet the needs of the position

- Basic computer skills

- Analytical, mathematical, and organizational skills

- Ability to follow instructions

- Ability to multitask

- Ability to listen, empathize and respond to patient, family,
internal and external partners needs

- Knowledge of medical terminology

- Ability to work with hazardous material and utilize personal
protective equipment

- Ability to maintain confidentiality



Benefits:



Relocation Assistance

PTO, 401(k), medical and dental plans.

Compensation and benefits offerings for affiliate employees go beyond
traditional benefit packages to give you more comprehensive coverage and
assistance for everything you need to stay happy and healthy at work and at
home.

Through a combination of core and elective benefits, offers industry-leading
compensation, comprehensive medical and dental coverage, generous retirement
plans, life insurance, short- and long-term disability plans, flexible
spending accounts, tuition assistance, continuing education and leadership
development, childcare assistance, adoption benefits, and discounts on
select goods and services.

Employee Health Assistance Fund, Promise Fund, and the Hope Fund.





TO APPLY

Please send updated resume, salary requirements to
Alyssa@alliedsearchpartners.com for initial prescreening. Thank you!


-- 

* *

**If you wish to no longer receive emails from Allied Search Partners please
reply with ?Remove.? *

Alyssa Peterson, Director of Candidate Recruitment
LinkedIN:http://www.linkedin.com/in/alyssapetersonasp

Allied Search Partners

T: 888.388.7571

F: 888.388.7572

www.alliedsearchpartners.com

This email including its attachments is intended only for the confidential
use of the individual to whom it is addressed. If you are not the intended
recipient, any use, dissemination, distribution or copying of this message
or its attachments is prohibited.  If you have received this message in
error, please notify us immediately, and delete this message and its
attachments permanently from your system.
From alyssa <@t> alliedsearchpartners.com  Mon Feb 21 10:33:26 2011
From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson)
Date: Mon Feb 21 10:33:34 2011
Subject: [Histonet] Histotech Needed on FL Gulf Coast
Message-ID: 

Position: Histology Technician, Histotechnician, Histotechnologist, Histologist

 Location: Panama City, FL



Schedule: Alternates between two shifts. Monday-Friday, 5am-1:30m, and
7am-3:30pm. Day Shift hours.



Description

 ?         Perform routine and complex histology procedures utilized
in pathology diagnosis.

?         Aid clinical practitioners in confirming or ruling out
diagnoses, monitoring chronic disease states, and analyzing the
effects of medical therapies.

 ESSENTIAL CORE FUNCTIONS:

?         Uses knowledge of laboratory science to perform the most
complex clinical testing as defined by the Clinical Laboratory
Improvement Amendments (CLIAA '88) providing safe and effective
patient care.

?         Maintains strict safety and infection control techniques at all times.

?         Practices, develops and endorses customer service skills in
relationships with internal and external customers.

?         Participate in Quality Improvement activities as assigned.

?         Actively seeks ways to control costs without compromising
patient safety, quality of care or the services delivered.

?         Follows established guidelines for reporting a significant
medical error or unanticipated outcome in the patient's care which
result in patient harm.

?         Uses occurrence reporting system to report potential patient
safety issues.

?         Demonstrates knowledge of Laboratory Information System.

?         Uses Performance Improvement Plan to improve patient safety.



Qualifications

 EDUCATION REQUIRED AND/OR PREFERRED:

- Vocational / Technical

- 2 year / Associate Degree

- 4 year/Bachelor's Degree

- License / Certification

 LICENSURE/CERTIFICATION:

- Florida Licensure as Clinical Laboratory Technologist / Supervisor /
Technician

 EXPERIENCE REQUIRED OR PREFERRED:

-1-3 years

 SKILLS, KNOWLEDGE, AND ABILITIES:

- Reads and writes Basic English possessing the basic skills necessary
to meet the needs of the position

- Basic computer skills

- Analytical, mathematical, and organizational skills

- Ability to follow instructions

- Ability to multitask

- Ability to listen, empathize and respond to patient, family,
internal and external partners needs

- Knowledge of medical terminology

- Ability to work with hazardous material and utilize personal
protective equipment

- Ability to maintain confidentiality



Benefits:



Relocation Assistance

PTO, 401(k), medical and dental plans.

Compensation and benefits offerings for affiliate employees go beyond
traditional benefit packages to give you more comprehensive coverage and
assistance for everything you need to stay happy and healthy at work and at
home.

Through a combination of core and elective benefits, offers industry-leading
compensation, comprehensive medical and dental coverage, generous retirement
plans, life insurance, short- and long-term disability plans, flexible
spending accounts, tuition assistance, continuing education and leadership
development, childcare assistance, adoption benefits, and discounts on
select goods and services.

Employee Health Assistance Fund, Promise Fund, and the Hope Fund.





TO APPLY

Please send updated resume, salary requirements to
Alyssa@alliedsearchpartners.com for initial prescreening. Thank you!


-- 

* *

**If you wish to no longer receive emails from Allied Search Partners please
reply with ?Remove.? *

Alyssa Peterson, Director of Candidate Recruitment
LinkedIN:http://www.linkedin.com/in/alyssapetersonasp

Allied Search Partners

T: 888.388.7571

F: 888.388.7572

www.alliedsearchpartners.com

This email including its attachments is intended only for the confidential
use of the individual to whom it is addressed. If you are not the intended
recipient, any use, dissemination, distribution or copying of this message
or its attachments is prohibited.  If you have received this message in
error, please notify us immediately, and delete this message and its
attachments permanently from your system.
From dgoodwin <@t> rwjuhh.edu  Mon Feb 21 10:39:34 2011
From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana)
Date: Mon Feb 21 10:39:44 2011
Subject: [Histonet] Bone marrow charges
Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71D1D05626D@HAMEXMBA.rwjham.local>

Greetings, Histonetters.

How many times can I charge 88313 for a bone marrow case that has an Iron stain on 2 separate blocks (one for the bx and one for the aspirate clot) and also on 1 smear made from the aspirate clot, a PAS on the 2 blocks, a Trichrome and a Retic on the bx. block, and a Wright/ Giemsa on 4 smears?

Thank you!!!
Diana Goodwin
Supervisor, Histology Laboratory
RWJUHHNJ


Diana Goodwin
Supervisor, Histology Laboratory
xt. 6996

From pruegg <@t> ihctech.net  Mon Feb 21 11:46:00 2011
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Mon Feb 21 11:46:48 2011
Subject: SPAM-LOW:  [Histonet] TRAP staining kit
In-Reply-To: <39735.94164.qm@web130120.mail.mud.yahoo.com>
References: <39735.94164.qm@web130120.mail.mud.yahoo.com>
Message-ID: 

Kim if you are asking if TRAP enzyme histochemistry works on formalin fixed
paraffin embedded decalcified bone in my experience it does not.  It works
on smears and cytology slides or GMA embedded bone without decal, it may
work on ETA decaled bone but I do not think it works on ffpe slides.  There
is an antibody to TRAP now you could use on ffpe sections by IHC.  What are
you trying to use it for, osteoclasts???

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Monday, February 21, 2011 7:39 AM
To: Histonet
Subject: SPAM-LOW: [Histonet] TRAP staining kit

Hi All,
?
Does anyone know if there is a TRAP staining kit available for histology
(and 
who sells it)?? I have?found a bunch of?them on google, but?they are all for

cell culture.
?
Thanks,
Kim
?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From ROrr <@t> northshore.org  Mon Feb 21 12:11:53 2011
From: ROrr <@t> northshore.org (Orr, Rebecca)
Date: Mon Feb 21 12:11:59 2011
Subject: [Histonet] Biocare Universal Negative for PIN4
In-Reply-To: <9055a5e3-586f-471b-8777-54f0db99182f@EXCHCAS02.enhnet.org>
References: <9055a5e3-586f-471b-8777-54f0db99182f@EXCHCAS02.enhnet.org>
Message-ID: 

I use this  in the same way as Mark  Tarango...
Here's the part number  NC498 L
800-799-7499 is the phone for Biocare  or www.biocare.net

They're great with tech support and can give prices and different sizes.

Becky Orr CLA,HT(ASCP)QIHC
Technical Specialist
Anatomic Pathology
NorthShore University HealthSystem
847-570-2771


Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s).  Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited.  Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights.  If you received this e-mail in error, please delete it immediately and notify the sender by return email.



From relia1 <@t> earthlink.net  Mon Feb 21 12:23:43 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Mon Feb 21 12:23:53 2011
Subject: [Histonet] A Quick update on some exciting new opportunites from
	Pam Barker at RELIA Solutions.
Message-ID: <0934A5B2347A4E599DB7BE3EA999CA52@ownerf1abaad51>

Hi Histonetters!!
If it is half as beautiful where you are today as it is here then
hooray!!  Spring is on it's way!!!  If it is nasty and cold where you
are take heart the worst is over it is downhill to Spring now and if you
are enjoying the snow then savor it because it will soon be gone!!
Anyway I wanted to take a minute to tell you about some new positions
that I am pretty excited about:
Here is a list of my newest histotech openings:

1.	Florida Licensed Histotechnologist - Day shift - Panama City, FL
2.	ASCP or Elig Histology Tech - day and evening shifts - Portland,
OR
3.	ASCP or Eligible Histology Tech - day shift - Tyler, TX
4.	Pathologists' Assistant - Charlotte, NC
5.	Histology Supervisor - Portland, ME

All of these opportunities are fulltime permanent positions and my
clients offer excellent compensation, benefits and relocation
assistance/sign on bonuses.
 
I also have histology supervisory positions in North Carolina, Maine and
WI and tech positions in TX, NY and MA.
 
If you or anyone you know might be interested in any of these
opportunities please contact me.  I can be reached toll free at
866-607-3542 or relia1@earthlink.net  
 
Have a great day!! Thanks-Pam
Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From nicholer <@t> slonepartners.com  Mon Feb 21 12:25:39 2011
From: nicholer <@t> slonepartners.com (Nichole Ramis)
Date: Mon Feb 21 12:25:48 2011
Subject: [Histonet] Seeking a Histotechnologist in DC
Message-ID: 

Slone Partners seeks a Histotechnologist for a cutting edge hospital
laboratory, based in a beautiful Washington DC neighborhood.
 
ASCP certification is preferred with at least 2 years of experience in a
high-volume laboratory.

Special features of the position:  This organization embraces curiosity and
enjoys mentoring and developing its employees.

Interested and qualified candidates should submit their resume to Kim Wilson
at kimd@slonepartners.com or by phone at 877-436-8105.


From fbozkurt <@t> gmail.com  Mon Feb 21 12:28:15 2011
From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT)
Date: Mon Feb 21 12:28:48 2011
Subject: [Histonet] about ki-67
Message-ID: 

Hello,

This is the first mail that i sent to this group. I'm looking for Ki-67
primary antibody for use on bovine tissue. I couldn't find on the internet
search. Does anyone know if there is a ki-67 primary antibody for available
IHC on bovine tissue ?

Best Regards,

Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-109
From hborgeri <@t> wfubmc.edu  Mon Feb 21 12:44:35 2011
From: hborgeri <@t> wfubmc.edu (Hermina Borgerink)
Date: Mon Feb 21 12:44:53 2011
Subject: SPAM-LOW:  [Histonet] TRAP staining kit
In-Reply-To: 
References: <39735.94164.qm@web130120.mail.mud.yahoo.com>
	
Message-ID: <1199C3D2DD5872438C656168966E32650245BA@EXCHDB1.medctr.ad.wfubmc.edu>

Hi Kim and Patsy,

This protocol works well on EDTA decalcified FFPE tissue samples.


Tartrate-Resistant Acid Phosphatase (TRAP) histochemistry

For Paraffin:
Deparaffinize sections through xylenes and alcohol and bring to running deionized water for 5 minutes. 
 
For Plastic:
Deplasticize through three 20-minute changes of 2-methoxyethyl acetate, two 5-minute changes of acetone followed by running deionized water for 5 minutes.
Make up acetate buffer in the volume needed for the number of slides to be stained.

0.2 M ACETATE BUFFER
Distilled water                                   for     50.0 ml         for  200.0 ml
0.2 M sodium acetate (FW 82.03)          0.82 gm                 3.28 gm
50 mM L(+) tartaric acid (FW 230.1)     0.58 gm                 2.32 gm
Stir using a magnetic stir bar until dissolved;  pH to 5.0

Following the 5-minute wash under running water, incubate the sections in 0.2 M acetate buffer for 20 minutes at room temperature.  After this time has elapsed, to this same acetate buffer add:

                                                                   for 50.0 ml         for 200.0 ml
0.5 mg/ml naphtol AS-MX phosphate     25.0 mg              100.0 mg 
1.1 mg/ml fast red TR salt                          55.0 mg               220.0 mg 

OR

Add the naphtol AS-MX phosphate and TR salt to freshly prepared and preheated  0.2 M sodium acetate buffer


Incubate the sections from 1 ? 4 hours at 37?C, monitoring after the first hour, until osteoclasts are bright red.  Rinse in distilled water, followed by counterstaining in Mayer?s hematoxylin for 1 ? 5 minutes (depending on the age of the hematoxylin), wash in running water for 5 minutes, air-dry and mount with permount.


Ref.  Erlebacher & Derynck J Cell Biol 132:  195 ? 210, 1996


September 30, 2003 (Revised)
Hermina Borgerink
Wake Forest University Health Sciences

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, February 21, 2011 12:46 PM
To: 'Kim Merriam'; 'Histonet'
Subject: RE: SPAM-LOW: [Histonet] TRAP staining kit

Kim if you are asking if TRAP enzyme histochemistry works on formalin fixed
paraffin embedded decalcified bone in my experience it does not.  It works
on smears and cytology slides or GMA embedded bone without decal, it may
work on ETA decaled bone but I do not think it works on ffpe slides.  There
is an antibody to TRAP now you could use on ffpe sections by IHC.  What are
you trying to use it for, osteoclasts???

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Monday, February 21, 2011 7:39 AM
To: Histonet
Subject: SPAM-LOW: [Histonet] TRAP staining kit

Hi All,
?
Does anyone know if there is a TRAP staining kit available for histology
(and 
who sells it)?? I have?found a bunch of?them on google, but?they are all for

cell culture.
?
Thanks,
Kim
?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From shive003 <@t> umn.edu  Mon Feb 21 12:58:40 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Mon Feb 21 12:58:44 2011
Subject: [Histonet] about ki-67
References: 
Message-ID: 

Hello, Dr. Bozkurt,

I know that Ki-67 from ThermoScientific (cat# RB-9043) works on bovine 
tissue, in my experience.  It does not stain a large quantity of cells in 
bovine tonsil, but those that do stain positively stain with moderate 
intensity.  It is a rabbit polyclonal antibody.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003@umn.edu

(Confidentiality Notice: This message, together with any attachments, is 
intended only for the use of the individual or entity to which it is 
addressed and may contain confidential or privileged information. If you 
think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)

----- Original Message ----- 
From: "Mehmet Fatih BOZKURT" 
To: 
Sent: Monday, February 21, 2011 12:28 PM
Subject: [Histonet] about ki-67


> Hello,
>
> This is the first mail that i sent to this group. I'm looking for Ki-67
> primary antibody for use on bovine tissue. I couldn't find on the internet
> search. Does anyone know if there is a ki-67 primary antibody for 
> available
> IHC on bovine tissue ?
>
> Best Regards,
>
> Mehmet Fatih BOZKURT, DVM, PhD
> Afyon Kocatepe University
> Faculty of Veterinary Medicine
> Department of Pathology
> 03030, ANS Campus
> Afyonkarahisar-TURKEY
> Tel: +902722281312-109
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From ross <@t> premierlab.com  Mon Feb 21 13:00:22 2011
From: ross <@t> premierlab.com (Ross Benik)
Date: Mon Feb 21 13:00:43 2011
Subject: [Histonet] about ki-67
In-Reply-To: 
Message-ID: 

Hi Mehmet,

We have used the Ki-67 antibody body from Santa Cruz Biotechnology
(sc-15402, rabbit poly) on multiple species such as human, rat, mouse
and canine.  We have not had the opportunity to test its cross
reactivity with bovine but it may be your best chance.   

-Ross



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet
Fatih BOZKURT
Sent: Monday, February 21, 2011 11:28 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] about ki-67

Hello,

This is the first mail that i sent to this group. I'm looking for Ki-67
primary antibody for use on bovine tissue. I couldn't find on the
internet
search. Does anyone know if there is a ki-67 primary antibody for
available
IHC on bovine tissue ?

Best Regards,

Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-109
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From liz <@t> premierlab.com  Mon Feb 21 13:06:59 2011
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Mon Feb 21 13:07:14 2011
Subject: SPAM-LOW:  [Histonet] TRAP staining kit
In-Reply-To: <1199C3D2DD5872438C656168966E32650245BA@EXCHDB1.medctr.ad.wfubmc.edu>
Message-ID: 

I'm in agreement with Patsy.  We have found in our hands that the Sigma Kit will not work on formic acid decaled bone.  It may work on EDTA decaled bone, the reason I say may is that we have gotten variable staining results with EDTA decaled bone.  For mouse knees and ankles we have gotten great results with EDTA decaled and paraffin processing.  We did modify the protocol a bit.  We tried it on EDTA decaled rat ankles and it did not work.  It might be due to the length of time in EDTA, I'm not sure, but bottom line it may not work.  At least that?s what our experience has been.  We also will use the TRAP antibody, we have found that it works in human mouse, and canine so far on formic acid decaled and paraffin embedded samples (and again I think length of time in decal may impact staining, the canine samples were teeth and surrounding soft tissue and it worked ok but not as well).  We have tried this antibody also on EDTA decaled mouse bone and it did not work, not sure why but in this instance we only received blocks so we did not have any control over fixation, decal or processing.  

If you do run into issues there are other markers out there that will stain osteoclasts, but are not entirely specific to them.  We have used the following antibodies;

Cathepsin K - works on formic acid and paraffin embedded in mouse and rat, but it may stain some inflammatory cells also
ED-1 - granted it?s a macrophage marker but it also work nicely to stain osteoclasts in rat bone, but again since it is a macrophage marker you are going to have to read through that.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hermina Borgerink
Sent: Monday, February 21, 2011 11:45 AM
To: Patsy Ruegg; 'Kim Merriam'; 'Histonet'
Subject: RE: SPAM-LOW: [Histonet] TRAP staining kit

Hi Kim and Patsy,

This protocol works well on EDTA decalcified FFPE tissue samples.


Tartrate-Resistant Acid Phosphatase (TRAP) histochemistry

For Paraffin:
Deparaffinize sections through xylenes and alcohol and bring to running deionized water for 5 minutes. 
 
For Plastic:
Deplasticize through three 20-minute changes of 2-methoxyethyl acetate, two 5-minute changes of acetone followed by running deionized water for 5 minutes.
Make up acetate buffer in the volume needed for the number of slides to be stained.

0.2 M ACETATE BUFFER
Distilled water                                   for     50.0 ml         for  200.0 ml
0.2 M sodium acetate (FW 82.03)          0.82 gm                 3.28 gm
50 mM L(+) tartaric acid (FW 230.1)     0.58 gm                 2.32 gm
Stir using a magnetic stir bar until dissolved;  pH to 5.0

Following the 5-minute wash under running water, incubate the sections in 0.2 M acetate buffer for 20 minutes at room temperature.  After this time has elapsed, to this same acetate buffer add:

                                                                   for 50.0 ml         for 200.0 ml
0.5 mg/ml naphtol AS-MX phosphate     25.0 mg              100.0 mg 
1.1 mg/ml fast red TR salt                          55.0 mg               220.0 mg 

OR

Add the naphtol AS-MX phosphate and TR salt to freshly prepared and preheated  0.2 M sodium acetate buffer


Incubate the sections from 1 ? 4 hours at 37?C, monitoring after the first hour, until osteoclasts are bright red.  Rinse in distilled water, followed by counterstaining in Mayer?s hematoxylin for 1 ? 5 minutes (depending on the age of the hematoxylin), wash in running water for 5 minutes, air-dry and mount with permount.


Ref.  Erlebacher & Derynck J Cell Biol 132:  195 ? 210, 1996


September 30, 2003 (Revised)
Hermina Borgerink
Wake Forest University Health Sciences

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, February 21, 2011 12:46 PM
To: 'Kim Merriam'; 'Histonet'
Subject: RE: SPAM-LOW: [Histonet] TRAP staining kit

Kim if you are asking if TRAP enzyme histochemistry works on formalin fixed
paraffin embedded decalcified bone in my experience it does not.  It works
on smears and cytology slides or GMA embedded bone without decal, it may
work on ETA decaled bone but I do not think it works on ffpe slides.  There
is an antibody to TRAP now you could use on ffpe sections by IHC.  What are
you trying to use it for, osteoclasts???

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Monday, February 21, 2011 7:39 AM
To: Histonet
Subject: SPAM-LOW: [Histonet] TRAP staining kit

Hi All,
?
Does anyone know if there is a TRAP staining kit available for histology
(and 
who sells it)?? I have?found a bunch of?them on google, but?they are all for

cell culture.
?
Thanks,
Kim
?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From pruegg <@t> ihctech.net  Mon Feb 21 13:16:03 2011
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Mon Feb 21 13:16:42 2011
Subject: SPAM-LOW:  RE: [Histonet] about ki-67
In-Reply-To: 
References: 
	
Message-ID: <203A52A882D44E3884C6738F22B154B8@prueggihctechlt>

I have not tried it on bovine but the NeoMarkers Rabbit monoclonal Ki67 has
worked on every other species I have tried, rat, mouse, human, guinea pig,
canine.

Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross Benik
Sent: Monday, February 21, 2011 12:00 PM
To: Mehmet Fatih BOZKURT; Histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] about ki-67

Hi Mehmet,

We have used the Ki-67 antibody body from Santa Cruz Biotechnology
(sc-15402, rabbit poly) on multiple species such as human, rat, mouse
and canine.  We have not had the opportunity to test its cross
reactivity with bovine but it may be your best chance.   

-Ross



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet
Fatih BOZKURT
Sent: Monday, February 21, 2011 11:28 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] about ki-67

Hello,

This is the first mail that i sent to this group. I'm looking for Ki-67
primary antibody for use on bovine tissue. I couldn't find on the
internet
search. Does anyone know if there is a ki-67 primary antibody for
available
IHC on bovine tissue ?

Best Regards,

Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-109
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From shive003 <@t> umn.edu  Mon Feb 21 13:21:46 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Mon Feb 21 13:21:49 2011
Subject: Fw: [Histonet] about ki-67
Message-ID: <4E4AC79AA1EE4A058CB879DCC4CC4D53@auxs.umn.edu>

I neglected to mention that in my experience ThermoScientific's antibody 
also works on dog, cat, pig, horse, rat, and primate; it does not work on 
birds.

Jan Shivers
Univ. of Minnesota

----- Original Message ----- 
From: "Jan Shivers" 
To: "Mehmet Fatih BOZKURT" ; 

Sent: Monday, February 21, 2011 12:58 PM
Subject: Re: [Histonet] about ki-67


> Hello, Dr. Bozkurt,
>
> I know that Ki-67 from ThermoScientific (cat# RB-9043) works on bovine 
> tissue, in my experience.  It does not stain a large quantity of cells in 
> bovine tonsil, but those that do stain positively stain with moderate 
> intensity.  It is a rabbit polyclonal antibody.
>
> Jan Shivers
> Senior Scientist
> Histology/IHC/EM Section Head
> Pathology Teaching Program
> University of Minnesota
> Veterinary Diagnostic Laboratory
> 1333 Gortner Ave.
> St. Paul, MN  55108
> 612-624-7297
> shive003@umn.edu
>
> (Confidentiality Notice: This message, together with any attachments, is 
> intended only for the use of the individual or entity to which it is 
> addressed and may contain confidential or privileged information. If you 
> think you have received this message in error, please advise the sender 
> and then delete this message and any attachments immediately.)
>
> ----- Original Message ----- 
> From: "Mehmet Fatih BOZKURT" 
> To: 
> Sent: Monday, February 21, 2011 12:28 PM
> Subject: [Histonet] about ki-67
>
>
>> Hello,
>>
>> This is the first mail that i sent to this group. I'm looking for Ki-67
>> primary antibody for use on bovine tissue. I couldn't find on the 
>> internet
>> search. Does anyone know if there is a ki-67 primary antibody for 
>> available
>> IHC on bovine tissue ?
>>
>> Best Regards,
>>
>> Mehmet Fatih BOZKURT, DVM, PhD
>> Afyon Kocatepe University
>> Faculty of Veterinary Medicine
>> Department of Pathology
>> 03030, ANS Campus
>> Afyonkarahisar-TURKEY
>> Tel: +902722281312-109
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From shive003 <@t> umn.edu  Mon Feb 21 13:23:01 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Mon Feb 21 13:23:07 2011
Subject: Fw: SPAM-LOW:  RE: [Histonet] about ki-67
Message-ID: <2DEEEB75FD274B1BAD9BB2F20F3F48A3@auxs.umn.edu>

Patsy, et al... NeoMarkers' antibody is the same one as ThermoScientific (TS 
owns NeoMarkers/LabVision antibodies).

Jan Shivers

----- Original Message ----- 
From: "Patsy Ruegg" 
To: "'Ross Benik'" ; "'Mehmet Fatih BOZKURT'" 
; 
Sent: Monday, February 21, 2011 1:16 PM
Subject: RE: SPAM-LOW: RE: [Histonet] about ki-67


>I have not tried it on bovine but the NeoMarkers Rabbit monoclonal Ki67 has
> worked on every other species I have tried, rat, mouse, human, guinea pig,
> canine.
>
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross Benik
> Sent: Monday, February 21, 2011 12:00 PM
> To: Mehmet Fatih BOZKURT; Histonet@lists.utsouthwestern.edu
> Subject: SPAM-LOW: RE: [Histonet] about ki-67
>
> Hi Mehmet,
>
> We have used the Ki-67 antibody body from Santa Cruz Biotechnology
> (sc-15402, rabbit poly) on multiple species such as human, rat, mouse
> and canine.  We have not had the opportunity to test its cross
> reactivity with bovine but it may be your best chance.
>
> -Ross
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet
> Fatih BOZKURT
> Sent: Monday, February 21, 2011 11:28 AM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] about ki-67
>
> Hello,
>
> This is the first mail that i sent to this group. I'm looking for Ki-67
> primary antibody for use on bovine tissue. I couldn't find on the
> internet
> search. Does anyone know if there is a ki-67 primary antibody for
> available
> IHC on bovine tissue ?
>
> Best Regards,
>
> Mehmet Fatih BOZKURT, DVM, PhD
> Afyon Kocatepe University
> Faculty of Veterinary Medicine
> Department of Pathology
> 03030, ANS Campus
> Afyonkarahisar-TURKEY
> Tel: +902722281312-109
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From pruegg <@t> ihctech.net  Mon Feb 21 13:29:57 2011
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Mon Feb 21 13:30:49 2011
Subject: SPAM-LOW:  RE: [Histonet] about ki-67
In-Reply-To: <2DEEEB75FD274B1BAD9BB2F20F3F48A3@auxs.umn.edu>
References: <2DEEEB75FD274B1BAD9BB2F20F3F48A3@auxs.umn.edu>
Message-ID: <76EC1452FE3541BCAC551D2095DCBB42@prueggihctechlt>

Yes you are right NeoMarkers is Thermo, but I am pretty sure it is a Rabbit
Monoclonal isn't it?

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: Monday, February 21, 2011 12:23 PM
To: histonet
Subject: Fw: SPAM-LOW: RE: [Histonet] about ki-67

Patsy, et al... NeoMarkers' antibody is the same one as ThermoScientific (TS

owns NeoMarkers/LabVision antibodies).

Jan Shivers

----- Original Message ----- 
From: "Patsy Ruegg" 
To: "'Ross Benik'" ; "'Mehmet Fatih BOZKURT'" 
; 
Sent: Monday, February 21, 2011 1:16 PM
Subject: RE: SPAM-LOW: RE: [Histonet] about ki-67


>I have not tried it on bovine but the NeoMarkers Rabbit monoclonal Ki67 has
> worked on every other species I have tried, rat, mouse, human, guinea pig,
> canine.
>
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross Benik
> Sent: Monday, February 21, 2011 12:00 PM
> To: Mehmet Fatih BOZKURT; Histonet@lists.utsouthwestern.edu
> Subject: SPAM-LOW: RE: [Histonet] about ki-67
>
> Hi Mehmet,
>
> We have used the Ki-67 antibody body from Santa Cruz Biotechnology
> (sc-15402, rabbit poly) on multiple species such as human, rat, mouse
> and canine.  We have not had the opportunity to test its cross
> reactivity with bovine but it may be your best chance.
>
> -Ross
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet
> Fatih BOZKURT
> Sent: Monday, February 21, 2011 11:28 AM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] about ki-67
>
> Hello,
>
> This is the first mail that i sent to this group. I'm looking for Ki-67
> primary antibody for use on bovine tissue. I couldn't find on the
> internet
> search. Does anyone know if there is a ki-67 primary antibody for
> available
> IHC on bovine tissue ?
>
> Best Regards,
>
> Mehmet Fatih BOZKURT, DVM, PhD
> Afyon Kocatepe University
> Faculty of Veterinary Medicine
> Department of Pathology
> 03030, ANS Campus
> Afyonkarahisar-TURKEY
> Tel: +902722281312-109
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From shive003 <@t> umn.edu  Mon Feb 21 13:55:27 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Mon Feb 21 13:55:30 2011
Subject: SPAM-LOW:  RE: [Histonet] about ki-67
References: <2DEEEB75FD274B1BAD9BB2F20F3F48A3@auxs.umn.edu>
	<76EC1452FE3541BCAC551D2095DCBB42@prueggihctechlt>
Message-ID: 

Patsy,
This particular ThermoScientific/NeoMarkers catalog number (RB-9043) is a 
rabbit polyclonal.
Jan

----- Original Message ----- 
From: "Patsy Ruegg" 
To: "'Jan Shivers'" ; "'histonet'" 

Sent: Monday, February 21, 2011 1:29 PM
Subject: RE: SPAM-LOW: RE: [Histonet] about ki-67


> Yes you are right NeoMarkers is Thermo, but I am pretty sure it is a 
> Rabbit
> Monoclonal isn't it?
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan 
> Shivers
> Sent: Monday, February 21, 2011 12:23 PM
> To: histonet
> Subject: Fw: SPAM-LOW: RE: [Histonet] about ki-67
>
> Patsy, et al... NeoMarkers' antibody is the same one as ThermoScientific 
> (TS
>
> owns NeoMarkers/LabVision antibodies).
>
> Jan Shivers
>
> ----- Original Message ----- 
> From: "Patsy Ruegg" 
> To: "'Ross Benik'" ; "'Mehmet Fatih BOZKURT'"
> ; 
> Sent: Monday, February 21, 2011 1:16 PM
> Subject: RE: SPAM-LOW: RE: [Histonet] about ki-67
>
>
>>I have not tried it on bovine but the NeoMarkers Rabbit monoclonal Ki67 
>>has
>> worked on every other species I have tried, rat, mouse, human, guinea 
>> pig,
>> canine.
>>
>> Patsy
>>
>> Patsy Ruegg, HT(ASCP)QIHC
>> IHCtech
>> 12635 Montview Blvd. Ste.215
>> Aurora, CO 80045
>> 720-859-4060
>> fax 720-859-4110
>> www.ihctech.net
>> www.ihcrg.org
>>
>>
>> -----Original Message-----
>> From: histonet-bounces@lists.utsouthwestern.edu
>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross 
>> Benik
>> Sent: Monday, February 21, 2011 12:00 PM
>> To: Mehmet Fatih BOZKURT; Histonet@lists.utsouthwestern.edu
>> Subject: SPAM-LOW: RE: [Histonet] about ki-67
>>
>> Hi Mehmet,
>>
>> We have used the Ki-67 antibody body from Santa Cruz Biotechnology
>> (sc-15402, rabbit poly) on multiple species such as human, rat, mouse
>> and canine.  We have not had the opportunity to test its cross
>> reactivity with bovine but it may be your best chance.
>>
>> -Ross
>>
>>
>>
>> -----Original Message-----
>> From: histonet-bounces@lists.utsouthwestern.edu
>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet
>> Fatih BOZKURT
>> Sent: Monday, February 21, 2011 11:28 AM
>> To: Histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] about ki-67
>>
>> Hello,
>>
>> This is the first mail that i sent to this group. I'm looking for Ki-67
>> primary antibody for use on bovine tissue. I couldn't find on the
>> internet
>> search. Does anyone know if there is a ki-67 primary antibody for
>> available
>> IHC on bovine tissue ?
>>
>> Best Regards,
>>
>> Mehmet Fatih BOZKURT, DVM, PhD
>> Afyon Kocatepe University
>> Faculty of Veterinary Medicine
>> Department of Pathology
>> 03030, ANS Campus
>> Afyonkarahisar-TURKEY
>> Tel: +902722281312-109
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> 


From mfisher <@t> ecrmc.org  Mon Feb 21 13:59:26 2011
From: mfisher <@t> ecrmc.org (Marcia Fisher)
Date: Mon Feb 21 13:59:35 2011
Subject: [Histonet] Decal Solution for Bone Marrow Core Biopsies
Message-ID: <3ACBB5D73A417547A01970CD3EB5509306FF750F@MAIL1.ecrmc.ci.el-centro.ca.us>

We would like to find another type of decal solution for our bone marrow
core biopsies.  The current solution, which is also used on routine bone
specimens, is leaching out the iron deposits.  What are others using for
BM and times used.  We currently use 45 minutes to one hour after
fixation.  These are adult BM cores only.  Thanking you in advance.  

 

M. Fisher

El Centro Regional Medical Center



ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.
 


From GrayN <@t> upstate.edu  Mon Feb 21 14:54:46 2011
From: GrayN <@t> upstate.edu (Noel Gray)
Date: Mon Feb 21 14:55:10 2011
Subject: [Histonet] Formalin-fixed paraffin embedded question
Message-ID: <4D628ACE.F981.0036.1@upstate.edu>

I am having an issue with formalin-fixed, paraffin embedded tissue that I am
sectioning. I am using a microtome to cut the tissue (mouse brain,
cerebellum and spinal cord) in 10 um sections which I will stain using
cressyl violet. Sometimes, the tissue in a block will splinter once it hits
the blade. Usually all samples from the same animal splinter but this is not
always the case. If I put the block into the water bath (sectioning surface
exposed to water or not) this seems to stop the splintering for 1-200 um
worth of tissue. However, I am afraid this may bring error into the
histological analysis of my tissue.
 
I assume it has something to do with the protocol I am using to prepare the
tissue. I guessed that maybe the dehydration, alcohol clearing, or paraffin
infiltration are not complete, resulting in the problem I have. However, I
looked at various FFPE protocols and each of my wash steps are longer, which
may be the problem? I was wondering if anyone has encountered this before,
or if anyone knows exactly what is going on with my tissue and how I can fix
it? Thank you. 
 
Here is my protocol:
 
-Anesthetize mouse followed by a system flush of 30 ml of PBS and then slow
perfusion of 50 ml of 4% PFA.
-Brain and spinal cord are removed as a single, in tact unit and placed
into 70% ethanol for 4 hours
-80% EtOH for 4 hours
-90% EtOH over night
-100% EtOH #1 for 4 hours
-100% EtOH #2 for 4 hours
-100% EtOH #3 over night, forebrain cerebellum and spinal cord are
separated
-Xylene wash #1 for 4 hours
-Xylene wash #2 for 4 hours
-Xylene wash #3 over night
-Moulton paraffin wash #1 for 4 hours
-Moulton paraffin wash #2 for 4 hours
-Moulton paraffin wash #3 over night
-Tissue is embedded and then sectioned into 10 um sections
 
Again thank you for your time.
 
Noel W Gray
Neuroscience Graduate Program
SUNY Upstate Medical University
3219 Weiskotten Hall
766 Irving Ave
Syracuse, NY 13210-1630
(315) 464-8144
grayn@upstate.edu
From rjbuesa <@t> yahoo.com  Mon Feb 21 14:59:18 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Mon Feb 21 14:59:22 2011
Subject: [Histonet] Formalin-fixed paraffin embedded question
In-Reply-To: <4D628ACE.F981.0036.1@upstate.edu>
Message-ID: <871036.69231.qm@web65709.mail.ac4.yahoo.com>

According with your protocol it seems that you are processing by hand. Even so, the periods seem too long, specially for brain using xylene.
The best (and "final") solution for your problem will be to abandon xylene and start using mineral oil.
Ren? J.

--- On Mon, 2/21/11, Noel Gray  wrote:


From: Noel Gray 
Subject: [Histonet] Formalin-fixed paraffin embedded question
To: histonet@lists.utsouthwestern.edu
Date: Monday, February 21, 2011, 3:54 PM


I am having an issue with formalin-fixed, paraffin embedded tissue that I am
sectioning. I am using a microtome to cut the tissue (mouse brain,
cerebellum and spinal cord) in 10 um sections which I will stain using
cressyl violet. Sometimes, the tissue in a block will splinter once it hits
the blade. Usually all samples from the same animal splinter but this is not
always the case. If I put the block into the water bath (sectioning surface
exposed to water or not) this seems to stop the splintering for 1-200 um
worth of tissue. However, I am afraid this may bring error into the
histological analysis of my tissue.

I assume it has something to do with the protocol I am using to prepare the
tissue. I guessed that maybe the dehydration, alcohol clearing, or paraffin
infiltration are not complete, resulting in the problem I have. However, I
looked at various FFPE protocols and each of my wash steps are longer, which
may be the problem? I was wondering if anyone has encountered this before,
or if anyone knows exactly what is going on with my tissue and how I can fix
it? Thank you. 

Here is my protocol:

-Anesthetize mouse followed by a system flush of 30 ml of PBS and then slow
perfusion of 50 ml of 4% PFA.
-Brain and spinal cord are removed as a single, in tact unit and placed
into 70% ethanol for 4 hours
-80% EtOH for 4 hours
-90% EtOH over night
-100% EtOH #1 for 4 hours
-100% EtOH #2 for 4 hours
-100% EtOH #3 over night, forebrain cerebellum and spinal cord are
separated
-Xylene wash #1 for 4 hours
-Xylene wash #2 for 4 hours
-Xylene wash #3 over night
-Moulton paraffin wash #1 for 4 hours
-Moulton paraffin wash #2 for 4 hours
-Moulton paraffin wash #3 over night
-Tissue is embedded and then sectioned into 10 um sections

Again thank you for your time.

Noel W Gray
Neuroscience Graduate Program
SUNY Upstate Medical University
3219 Weiskotten Hall
766 Irving Ave
Syracuse, NY 13210-1630
(315) 464-8144
grayn@upstate.edu
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From fbozkurt <@t> gmail.com  Mon Feb 21 15:02:01 2011
From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT)
Date: Mon Feb 21 15:02:04 2011
Subject: [Histonet] Re: about ki-67
In-Reply-To: 
References: 
Message-ID: 

 Then I have two options. Firstly i have to try  ThermoScientific's
antibody. for alternative SC antibody. Thank you for your answers.

Regards.

Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-109


On Mon, Feb 21, 2011 at 8:28 PM, Mehmet Fatih BOZKURT wrote:

> Hello,
>
> This is the first mail that i sent to this group. I'm looking for Ki-67
> primary antibody for use on bovine tissue. I couldn't find on the internet
> search. Does anyone know if there is a ki-67 primary antibody for available
> IHC on bovine tissue ?
>
> Best Regards,
>
> Mehmet Fatih BOZKURT, DVM, PhD
> Afyon Kocatepe University
> Faculty of Veterinary Medicine
> Department of Pathology
> 03030, ANS Campus
> Afyonkarahisar-TURKEY
> Tel: +902722281312-109
>
>
From sgoebel <@t> mirnarx.com  Mon Feb 21 15:17:54 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Mon Feb 21 15:17:58 2011
Subject: [Histonet] Formalin-fixed paraffin embedded question
In-Reply-To: <4D628ACE.F981.0036.1@upstate.edu>
References: <4D628ACE.F981.0036.1@upstate.edu>
Message-ID: 

Wow!!  Processing by hand...I didn't think people actually did this
anymore?  I think what your problem might be is that you are not fixing
the tissue before you submerge it into alcohol for those long periods of
time.  The "splintering" artifact as you describe probably means that
your tissues are severely over dehydrated.  This is why when you float
them in the water bath for a minute or two it goes away for awhile in
the block (basically until you get to the over dehydrated tissue again).
Try fixing the samples in 10% formalin for at least overnight before you
dunk them into alcohol.
Good Luck!!

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Noel
Gray
Sent: Monday, February 21, 2011 2:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin-fixed paraffin embedded question

I am having an issue with formalin-fixed, paraffin embedded tissue that
I am
sectioning. I am using a microtome to cut the tissue (mouse brain,
cerebellum and spinal cord) in 10 um sections which I will stain using
cressyl violet. Sometimes, the tissue in a block will splinter once it
hits
the blade. Usually all samples from the same animal splinter but this is
not
always the case. If I put the block into the water bath (sectioning
surface
exposed to water or not) this seems to stop the splintering for 1-200 um
worth of tissue. However, I am afraid this may bring error into the
histological analysis of my tissue.
 
I assume it has something to do with the protocol I am using to prepare
the
tissue. I guessed that maybe the dehydration, alcohol clearing, or
paraffin
infiltration are not complete, resulting in the problem I have. However,
I
looked at various FFPE protocols and each of my wash steps are longer,
which
may be the problem? I was wondering if anyone has encountered this
before,
or if anyone knows exactly what is going on with my tissue and how I can
fix
it? Thank you. 
 
Here is my protocol:
 
-Anesthetize mouse followed by a system flush of 30 ml of PBS and then
slow
perfusion of 50 ml of 4% PFA.
-Brain and spinal cord are removed as a single, in tact unit and placed
into 70% ethanol for 4 hours
-80% EtOH for 4 hours
-90% EtOH over night
-100% EtOH #1 for 4 hours
-100% EtOH #2 for 4 hours
-100% EtOH #3 over night, forebrain cerebellum and spinal cord are
separated
-Xylene wash #1 for 4 hours
-Xylene wash #2 for 4 hours
-Xylene wash #3 over night
-Moulton paraffin wash #1 for 4 hours
-Moulton paraffin wash #2 for 4 hours
-Moulton paraffin wash #3 over night
-Tissue is embedded and then sectioned into 10 um sections
 
Again thank you for your time.
 
Noel W Gray
Neuroscience Graduate Program
SUNY Upstate Medical University
3219 Weiskotten Hall
766 Irving Ave
Syracuse, NY 13210-1630
(315) 464-8144
grayn@upstate.edu
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From algranth <@t> email.arizona.edu  Mon Feb 21 16:25:22 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Mon Feb 21 16:25:38 2011
Subject: [Histonet] Formalin-fixed paraffin embedded question
In-Reply-To: <4D628ACE.F981.0036.1@upstate.edu>
References: <4D628ACE.F981.0036.1@upstate.edu>
Message-ID: <0DA532A1-2516-4EC4-8D50-4653A5928BCC@email.arizona.edu>

Noel,
I agree. Your tissue isn't fixed well enough and the dehydration steps seem long, especially since at some point you split the brain and the spinal cord. After perfusion immerse in 10% NBF (a few changes) at least 24 hrs (I like to leave it in formalin longer). If you are fixing the brain whole remember that formalin is a slow fixative and it takes some time to penetrate all the way into the brain even if it was perfused first. I found that by combining perfusion with immersion in the fixative the brain fixed and cut better. Before you start dehydrating, try washing the tissue in running water at least an hour (or longer). Then start your dehydration.
Xylene makes tissue brittle and overnite in xylene I think isn't doing you any favors. I use Clear Rite 3 for my clearing agent. 
Are you hand processing? Do do have access to vacuum infiltration? I have a protocol for mouse brains but it is for processing on a VIP.
When cutting try soaking the block at room temp in water to which a bit of glycerin has been added. I usually don't place the brains on ice but just in cool water and my room is always cold.
I read here on histonet that people let their tissues soak for a few minutes but with mouse tissue you need to soak it longer. I don't really time the soaking but it is way longer than a few minutes. Then you might try wiping the surface of the block with a gauze sponge or even a cotton tipped applicator that was dipped in the soaking water in between sections as you are cutting your ribbon - don't make it too wet just moist.

Andi




On Feb 21, 2011, at 1:54 PM, Noel Gray wrote:

> I am having an issue with formalin-fixed, paraffin embedded tissue that I am
> sectioning. I am using a microtome to cut the tissue (mouse brain,
> cerebellum and spinal cord) in 10 um sections which I will stain using
> cressyl violet. Sometimes, the tissue in a block will splinter once it hits
> the blade. Usually all samples from the same animal splinter but this is not
> always the case. If I put the block into the water bath (sectioning surface
> exposed to water or not) this seems to stop the splintering for 1-200 um
> worth of tissue. However, I am afraid this may bring error into the
> histological analysis of my tissue.
> 
> I assume it has something to do with the protocol I am using to prepare the
> tissue. I guessed that maybe the dehydration, alcohol clearing, or paraffin
> infiltration are not complete, resulting in the problem I have. However, I
> looked at various FFPE protocols and each of my wash steps are longer, which
> may be the problem? I was wondering if anyone has encountered this before,
> or if anyone knows exactly what is going on with my tissue and how I can fix
> it? Thank you. 
> 
> Here is my protocol:
> 
> -Anesthetize mouse followed by a system flush of 30 ml of PBS and then slow
> perfusion of 50 ml of 4% PFA.
> -Brain and spinal cord are removed as a single, in tact unit and placed
> into 70% ethanol for 4 hours
> -80% EtOH for 4 hours
> -90% EtOH over night
> -100% EtOH #1 for 4 hours
> -100% EtOH #2 for 4 hours
> -100% EtOH #3 over night, forebrain cerebellum and spinal cord are
> separated
> -Xylene wash #1 for 4 hours
> -Xylene wash #2 for 4 hours
> -Xylene wash #3 over night
> -Moulton paraffin wash #1 for 4 hours
> -Moulton paraffin wash #2 for 4 hours
> -Moulton paraffin wash #3 over night
> -Tissue is embedded and then sectioned into 10 um sections
> 
> Again thank you for your time.
> 
> Noel W Gray
> Neuroscience Graduate Program
> SUNY Upstate Medical University
> 3219 Weiskotten Hall
> 766 Irving Ave
> Syracuse, NY 13210-1630
> (315) 464-8144
> grayn@upstate.edu
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From micro <@t> superlink.net  Mon Feb 21 20:52:03 2011
From: micro <@t> superlink.net (Markus F. Meyenhofer)
Date: Mon Feb 21 20:52:11 2011
Subject: [Histonet] LKB Bromma 7800 knifemaker
References: <375198e49e84933b6323635edb4609dc.squirrel@webmail.rci.rutgers.edu>
Message-ID: <2DEE5638FCF84462A7D64572441980C0@DJ4VDH31>

I recondition and repair LKB Knife Makers.
Reconditioning $350.00 plus shipping.
In NJ, I will pick up and deliver for the above recond. price.

Regards,
Markus F. Meyenhofer
Microscopy Labs
Box 338
Red Bank, NJ 07701
732 747 6228
micro@superlink.net

----- Original Message ----- 
From: 
To: "histonet" 
Sent: Friday, February 18, 2011 4:38 PM
Subject: [Histonet] LKB Bromma 7800 knifemaker



Yes, I know this is ancient.  But does anyone know of a service company
that can repair this machine?  Ours works, sort of, but it's very
frustrating.

Thanks and have a good weekend,
Kathleen Roberts

Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From EstepJK <@t> msha.com  Mon Feb 21 22:04:29 2011
From: EstepJK <@t> msha.com (Estep, Jennifer)
Date: Mon Feb 21 22:04:39 2011
Subject: [Histonet] Looking for Histology Tech position near Martinsville VA
Message-ID: 


         Hello! My name is Jennifer Estep. I am currently working as a histology technician at Johnson City Medical Center- Mountain States Health Alliance, in Johnson City Tennessee. I have been in this position for three years.  I am not certified yet, I have two more semesters of school left, and then will be able to sit for my exam. While holding this position I have gone through a year long Histology program that was offered by our Laboratory. I have experience with Special and IHC staining and frozen sections. Before working as a histology tech, I had worked in the laboratory as a phlebotomist, specimen processor, and pathology technician.
        I am relocating to Martinsville, VA as soon as I am able to find a full-time position. I am looking for a position within an hour from Martinsville. Roanoke, VA; Eden, NC; Greensboro, NC; or Danville, VA. I am available to work any shifts and can provide references along with letters of recommendations. If anyone knows of a position or has a position available, please contact me by email at EstepJK@MSHA.com or phone (423)943-8202.

                                                                                                                                                        Thanks, Jennifer Estep
                                                                                                                                                                    Histology Dept.
                                                                                                                                                                     JCMC-MSHA
                                                                                                                                                                    (423)431-6385




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From mab70 <@t> medschl.cam.ac.uk  Tue Feb 22 03:02:04 2011
From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount)
Date: Tue Feb 22 03:04:03 2011
Subject: [Histonet] Formalin-fixed paraffin embedded question
In-Reply-To: <4D628ACE.F981.0036.1@upstate.edu>
References: <4D628ACE.F981.0036.1@upstate.edu>
Message-ID: 

I agree with all the comments so far, and I think all the steps are far
too long, particularly the overnight in molten wax. You are effectively
cooking your samples. I would avoid overnight in 100% ethanol too; it
will tend to harden the tissue.
Get hold of the manual for animal tissues available from the Society for
histotechnology. I found this invaluable and now most of my tissues can
be sectioned without soaking at all. I just chill them slightly, if
necessary.

Good luck with your samples.

Margaret

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Noel
Gray
Sent: 21 February 2011 20:55
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin-fixed paraffin embedded question

I am having an issue with formalin-fixed, paraffin embedded tissue that
I am
sectioning. I am using a microtome to cut the tissue (mouse brain,
cerebellum and spinal cord) in 10 um sections which I will stain using
cressyl violet. Sometimes, the tissue in a block will splinter once it
hits
the blade. Usually all samples from the same animal splinter but this is
not
always the case. If I put the block into the water bath (sectioning
surface
exposed to water or not) this seems to stop the splintering for 1-200 um
worth of tissue. However, I am afraid this may bring error into the
histological analysis of my tissue.
 
I assume it has something to do with the protocol I am using to prepare
the
tissue. I guessed that maybe the dehydration, alcohol clearing, or
paraffin
infiltration are not complete, resulting in the problem I have. However,
I
looked at various FFPE protocols and each of my wash steps are longer,
which
may be the problem? I was wondering if anyone has encountered this
before,
or if anyone knows exactly what is going on with my tissue and how I can
fix
it? Thank you. 
 
Here is my protocol:
 
-Anesthetize mouse followed by a system flush of 30 ml of PBS and then
slow
perfusion of 50 ml of 4% PFA.
-Brain and spinal cord are removed as a single, in tact unit and placed
into 70% ethanol for 4 hours
-80% EtOH for 4 hours
-90% EtOH over night
-100% EtOH #1 for 4 hours
-100% EtOH #2 for 4 hours
-100% EtOH #3 over night, forebrain cerebellum and spinal cord are
separated
-Xylene wash #1 for 4 hours
-Xylene wash #2 for 4 hours
-Xylene wash #3 over night
-Moulton paraffin wash #1 for 4 hours
-Moulton paraffin wash #2 for 4 hours
-Moulton paraffin wash #3 over night
-Tissue is embedded and then sectioned into 10 um sections
 
Again thank you for your time.
 
Noel W Gray
Neuroscience Graduate Program
SUNY Upstate Medical University
3219 Weiskotten Hall
766 Irving Ave
Syracuse, NY 13210-1630
(315) 464-8144
grayn@upstate.edu
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rsrichmond <@t> gmail.com  Tue Feb 22 06:43:39 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Tue Feb 22 06:43:43 2011
Subject: [Histonet] Re: Decal Solution for Bone Marrow Core Biopsies
Message-ID: 

Marcia Fisher at El Centro Regional Medical Center (at the
southeastern tip of California) asks:

>>We would like to find another type of decal solution for our bone marrow core biopsies. The current solution, which is also used on routine bone specimens, is leaching out the iron deposits. What are others using for BM and times used? We currently use 45 minutes to one hour after fixation. These are adult BM cores only.<<

Any practical decalcifier is going to remove some hemosiderin iron.
You need to do the iron stain (Perls prussian blue reaction) on a
smear AND on the clot section or marrow particle section.

"Decal" is the name of a proprietary decalcifier. It should not be
used as a generic term for a decalcifying solution.

Bob Richmond
Samurai Pathologist
Knoxville TN

From lori.w <@t> sympatico.ca  Tue Feb 22 07:09:26 2011
From: lori.w <@t> sympatico.ca (L White)
Date: Tue Feb 22 07:09:22 2011
Subject: [Histonet] Re:  Tissue Processors
In-Reply-To: <20110218180655.RDGE878.tomts28-srv.bellnexxia.net@toip30.srvr.bell.ca>
References: <20110218180655.RDGE878.tomts28-srv.bellnexxia.net@toip30.srvr.bell.ca>
Message-ID: 

Hi Joe,
We just completed an extensive tissue processor evaluation.  We have an
Excelsior and use it exclusively for biopsies.  It is a good processor with
very little hands-on maintenance, with the one exception that you cannot
access the reagent bottles for regular cleaning.  This becomes a problem if
using for larger, fatty tissues.  My concern with the Peloris is the
possibility of downtime affecting both retorts.  We do not have the luxury
of purchasing two dual retort processors and I could not be without the
capacity of the two retorts for any length of time.  You can't beat Sakura
for tried and true technology (we have 2 old VIP K series still kicking) and
excellent service.
Good luck in your decision.

Lori W.




----------------------------------------------------------------------

Message: 1
Date: Thu, 17 Feb 2011 19:55:53 -0600
From: "Joe Nocito" 
Subject: [Histonet] Tissue Processors
To: "Histonet" 
Message-ID: <48ABF837D7084DCCB45CADF5F2B3F20A@JoePC>
Content-Type: text/plain;	charset="iso-8859-1"

Greetings all,
if you had to purchase new tissue processors, which one would you choose?
Microwave technology is out of the question. Are Sakura's still a good buy?
We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your
help

Joe



From Farnana <@t> nehealth.com  Tue Feb 22 09:11:55 2011
From: Farnana <@t> nehealth.com (Amy Farnan)
Date: Tue Feb 22 09:12:01 2011
Subject: [Histonet] NYSHS Meeting
Message-ID: <4D638BEB.26ED.00D9.1@nehealth.com>

Hello vendors!
 
The NYSHS State Meeting is being held May 14th at the Desmond in Albany.
If you would like to represent your company at our meeting and rent a booth please contact our vendor co-ordinator Linda Chen at kooki55@hotmail.com for more information.
 
Thank you

Disclaimer: The information in this message is confidential.  If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender.
From mhale <@t> carisls.com  Tue Feb 22 09:19:09 2011
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Tue Feb 22 09:19:13 2011
Subject: [Histonet] New Mexico HT Position 
Message-ID: <6F33D8418806044682A391273399860F070D28E3@s-irv-ex301.PathologyPartners.intranet>

Great opportunity for a Histotechnician in a brand new laboratory!
Digestive Disease Consultants of Las Cruces , NM  is looking for a
certified HT or HTL to run their newly constructed laboratory. Candidate
must be ASCP certified and CLIA certified to perform gross dissection,
prior supervisory experience preferred. The candidate will be
responsible for the following: Creation and maintenance of policies and
procedures to CLIA standards, leading lab through CLIA inspection,
maintenance and quality control for equipment, and routine histology
duties. This is a full time position that offers a competitive salary
and the flexible hours allow you to put your own personal stamp on the
laboratory. This will be a part time position .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mhale@carisls.com 

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mhale@carisls.com   

 

From algranth <@t> email.arizona.edu  Tue Feb 22 09:48:08 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Tue Feb 22 09:48:18 2011
Subject: [Histonet] Formalin-fixed paraffin embedded question
In-Reply-To: 
References: <4D628ACE.F981.0036.1@upstate.edu>
	
Message-ID: <74AE8774-91FA-4A2D-A698-A6D819AFD297@email.arizona.edu>

The NSH Manual needs to be seriously updated. There is no protocol for mouse brain that could help this guy with his problem.

Andi



On Feb 22, 2011, at 2:02 AM, Margaret Blount wrote:

> I agree with all the comments so far, and I think all the steps are far
> too long, particularly the overnight in molten wax. You are effectively
> cooking your samples. I would avoid overnight in 100% ethanol too; it
> will tend to harden the tissue.
> Get hold of the manual for animal tissues available from the Society for
> histotechnology. I found this invaluable and now most of my tissues can
> be sectioned without soaking at all. I just chill them slightly, if
> necessary.
> 
> Good luck with your samples.
> 
> Margaret
> 
> Miss Margaret Blount
> Histology Manager
> Metabolic Research Laboratories
> Level 4 Institute of Metabolic Science
> Box 289, Addenbrooke's Hospital
> Hills Road, Cambridge, CB2 0QQ
> 
> Tel 01223 769061/336079
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Noel
> Gray
> Sent: 21 February 2011 20:55
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Formalin-fixed paraffin embedded question
> 
> I am having an issue with formalin-fixed, paraffin embedded tissue that
> I am
> sectioning. I am using a microtome to cut the tissue (mouse brain,
> cerebellum and spinal cord) in 10 um sections which I will stain using
> cressyl violet. Sometimes, the tissue in a block will splinter once it
> hits
> the blade. Usually all samples from the same animal splinter but this is
> not
> always the case. If I put the block into the water bath (sectioning
> surface
> exposed to water or not) this seems to stop the splintering for 1-200 um
> worth of tissue. However, I am afraid this may bring error into the
> histological analysis of my tissue.
> 
> I assume it has something to do with the protocol I am using to prepare
> the
> tissue. I guessed that maybe the dehydration, alcohol clearing, or
> paraffin
> infiltration are not complete, resulting in the problem I have. However,
> I
> looked at various FFPE protocols and each of my wash steps are longer,
> which
> may be the problem? I was wondering if anyone has encountered this
> before,
> or if anyone knows exactly what is going on with my tissue and how I can
> fix
> it? Thank you. 
> 
> Here is my protocol:
> 
> -Anesthetize mouse followed by a system flush of 30 ml of PBS and then
> slow
> perfusion of 50 ml of 4% PFA.
> -Brain and spinal cord are removed as a single, in tact unit and placed
> into 70% ethanol for 4 hours
> -80% EtOH for 4 hours
> -90% EtOH over night
> -100% EtOH #1 for 4 hours
> -100% EtOH #2 for 4 hours
> -100% EtOH #3 over night, forebrain cerebellum and spinal cord are
> separated
> -Xylene wash #1 for 4 hours
> -Xylene wash #2 for 4 hours
> -Xylene wash #3 over night
> -Moulton paraffin wash #1 for 4 hours
> -Moulton paraffin wash #2 for 4 hours
> -Moulton paraffin wash #3 over night
> -Tissue is embedded and then sectioned into 10 um sections
> 
> Again thank you for your time.
> 
> Noel W Gray
> Neuroscience Graduate Program
> SUNY Upstate Medical University
> 3219 Weiskotten Hall
> 766 Irving Ave
> Syracuse, NY 13210-1630
> (315) 464-8144
> grayn@upstate.edu
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From sgoebel <@t> mirnarx.com  Tue Feb 22 09:52:19 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Tue Feb 22 09:52:23 2011
Subject: [Histonet] Formalin-fixed paraffin embedded question
In-Reply-To: 
References: <4D628ACE.F981.0036.1@upstate.edu>
	
Message-ID: 

Just got to thinking...I do animal tissue processing.  I do use an
automated processor, but it is open with no pressure or vacuum.  If you
think this will help I can give you the times I use.  It is an overnight
process and takes more than a work day worth of hours.  Let me know?

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret
Blount
Sent: Tuesday, February 22, 2011 3:02 AM
To: Noel Gray; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Formalin-fixed paraffin embedded question

I agree with all the comments so far, and I think all the steps are far
too long, particularly the overnight in molten wax. You are effectively
cooking your samples. I would avoid overnight in 100% ethanol too; it
will tend to harden the tissue.
Get hold of the manual for animal tissues available from the Society for
histotechnology. I found this invaluable and now most of my tissues can
be sectioned without soaking at all. I just chill them slightly, if
necessary.

Good luck with your samples.

Margaret

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Noel
Gray
Sent: 21 February 2011 20:55
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin-fixed paraffin embedded question

I am having an issue with formalin-fixed, paraffin embedded tissue that
I am
sectioning. I am using a microtome to cut the tissue (mouse brain,
cerebellum and spinal cord) in 10 um sections which I will stain using
cressyl violet. Sometimes, the tissue in a block will splinter once it
hits
the blade. Usually all samples from the same animal splinter but this is
not
always the case. If I put the block into the water bath (sectioning
surface
exposed to water or not) this seems to stop the splintering for 1-200 um
worth of tissue. However, I am afraid this may bring error into the
histological analysis of my tissue.
 
I assume it has something to do with the protocol I am using to prepare
the
tissue. I guessed that maybe the dehydration, alcohol clearing, or
paraffin
infiltration are not complete, resulting in the problem I have. However,
I
looked at various FFPE protocols and each of my wash steps are longer,
which
may be the problem? I was wondering if anyone has encountered this
before,
or if anyone knows exactly what is going on with my tissue and how I can
fix
it? Thank you. 
 
Here is my protocol:
 
-Anesthetize mouse followed by a system flush of 30 ml of PBS and then
slow
perfusion of 50 ml of 4% PFA.
-Brain and spinal cord are removed as a single, in tact unit and placed
into 70% ethanol for 4 hours
-80% EtOH for 4 hours
-90% EtOH over night
-100% EtOH #1 for 4 hours
-100% EtOH #2 for 4 hours
-100% EtOH #3 over night, forebrain cerebellum and spinal cord are
separated
-Xylene wash #1 for 4 hours
-Xylene wash #2 for 4 hours
-Xylene wash #3 over night
-Moulton paraffin wash #1 for 4 hours
-Moulton paraffin wash #2 for 4 hours
-Moulton paraffin wash #3 over night
-Tissue is embedded and then sectioned into 10 um sections
 
Again thank you for your time.
 
Noel W Gray
Neuroscience Graduate Program
SUNY Upstate Medical University
3219 Weiskotten Hall
766 Irving Ave
Syracuse, NY 13210-1630
(315) 464-8144
grayn@upstate.edu
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From sgoebel <@t> mirnarx.com  Tue Feb 22 09:53:35 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Tue Feb 22 09:53:39 2011
Subject: [Histonet] Formalin-fixed paraffin embedded question
In-Reply-To: <74AE8774-91FA-4A2D-A698-A6D819AFD297@email.arizona.edu>
References: <4D628ACE.F981.0036.1@upstate.edu>
	<74AE8774-91FA-4A2D-A698-A6D819AFD297@email.arizona.edu>
Message-ID: 

Are you volunteering?  That is so nice of you =)

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Grantham, Andrea L - (algranth)
Sent: Tuesday, February 22, 2011 9:48 AM
To: Margaret Blount
Cc: Noel Gray; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Formalin-fixed paraffin embedded question

The NSH Manual needs to be seriously updated. There is no protocol for
mouse brain that could help this guy with his problem.

Andi



On Feb 22, 2011, at 2:02 AM, Margaret Blount wrote:

> I agree with all the comments so far, and I think all the steps are 
> far too long, particularly the overnight in molten wax. You are 
> effectively cooking your samples. I would avoid overnight in 100% 
> ethanol too; it will tend to harden the tissue.
> Get hold of the manual for animal tissues available from the Society 
> for histotechnology. I found this invaluable and now most of my 
> tissues can be sectioned without soaking at all. I just chill them 
> slightly, if necessary.
> 
> Good luck with your samples.
> 
> Margaret
> 
> Miss Margaret Blount
> Histology Manager
> Metabolic Research Laboratories
> Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital

> Hills Road, Cambridge, CB2 0QQ
> 
> Tel 01223 769061/336079
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Noel 
> Gray
> Sent: 21 February 2011 20:55
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Formalin-fixed paraffin embedded question
> 
> I am having an issue with formalin-fixed, paraffin embedded tissue 
> that I am sectioning. I am using a microtome to cut the tissue (mouse 
> brain, cerebellum and spinal cord) in 10 um sections which I will 
> stain using cressyl violet. Sometimes, the tissue in a block will 
> splinter once it hits the blade. Usually all samples from the same 
> animal splinter but this is not always the case. If I put the block 
> into the water bath (sectioning surface exposed to water or not) this 
> seems to stop the splintering for 1-200 um worth of tissue. However, I

> am afraid this may bring error into the histological analysis of my 
> tissue.
> 
> I assume it has something to do with the protocol I am using to 
> prepare the tissue. I guessed that maybe the dehydration, alcohol 
> clearing, or paraffin infiltration are not complete, resulting in the 
> problem I have. However, I looked at various FFPE protocols and each 
> of my wash steps are longer, which may be the problem? I was wondering

> if anyone has encountered this before, or if anyone knows exactly what

> is going on with my tissue and how I can fix it? Thank you.
> 
> Here is my protocol:
> 
> -Anesthetize mouse followed by a system flush of 30 ml of PBS and then

> slow perfusion of 50 ml of 4% PFA.
> -Brain and spinal cord are removed as a single, in tact unit and 
> placed into 70% ethanol for 4 hours -80% EtOH for 4 hours -90% EtOH 
> over night -100% EtOH #1 for 4 hours -100% EtOH #2 for 4 hours -100% 
> EtOH #3 over night, forebrain cerebellum and spinal cord are separated

> -Xylene wash #1 for 4 hours -Xylene wash #2 for 4 hours -Xylene wash 
> #3 over night -Moulton paraffin wash #1 for 4 hours -Moulton paraffin 
> wash #2 for 4 hours -Moulton paraffin wash #3 over night -Tissue is 
> embedded and then sectioned into 10 um sections
> 
> Again thank you for your time.
> 
> Noel W Gray
> Neuroscience Graduate Program
> SUNY Upstate Medical University
> 3219 Weiskotten Hall
> 766 Irving Ave
> Syracuse, NY 13210-1630
> (315) 464-8144
> grayn@upstate.edu
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From algranth <@t> email.arizona.edu  Tue Feb 22 09:57:11 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Tue Feb 22 09:57:23 2011
Subject: [Histonet] Formalin-fixed paraffin embedded question
In-Reply-To: 
References: <4D628ACE.F981.0036.1@upstate.edu>
	<74AE8774-91FA-4A2D-A698-A6D819AFD297@email.arizona.edu>
	
Message-ID: 

I'd be happy to help. Maybe you can too! 8-)



On Feb 22, 2011, at 8:53 AM,  wrote:

> Are you volunteering?  That is so nice of you =)
> 
> Sarah Goebel, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> Grantham, Andrea L - (algranth)
> Sent: Tuesday, February 22, 2011 9:48 AM
> To: Margaret Blount
> Cc: Noel Gray; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Formalin-fixed paraffin embedded question
> 
> The NSH Manual needs to be seriously updated. There is no protocol for
> mouse brain that could help this guy with his problem.
> 
> Andi
> 
> 
> 
> On Feb 22, 2011, at 2:02 AM, Margaret Blount wrote:
> 
>> I agree with all the comments so far, and I think all the steps are 
>> far too long, particularly the overnight in molten wax. You are 
>> effectively cooking your samples. I would avoid overnight in 100% 
>> ethanol too; it will tend to harden the tissue.
>> Get hold of the manual for animal tissues available from the Society 
>> for histotechnology. I found this invaluable and now most of my 
>> tissues can be sectioned without soaking at all. I just chill them 
>> slightly, if necessary.
>> 
>> Good luck with your samples.
>> 
>> Margaret
>> 
>> Miss Margaret Blount
>> Histology Manager
>> Metabolic Research Laboratories
>> Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital
> 
>> Hills Road, Cambridge, CB2 0QQ
>> 
>> Tel 01223 769061/336079
>> 
>> 
>> -----Original Message-----
>> From: histonet-bounces@lists.utsouthwestern.edu
>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Noel 
>> Gray
>> Sent: 21 February 2011 20:55
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] Formalin-fixed paraffin embedded question
>> 
>> I am having an issue with formalin-fixed, paraffin embedded tissue 
>> that I am sectioning. I am using a microtome to cut the tissue (mouse 
>> brain, cerebellum and spinal cord) in 10 um sections which I will 
>> stain using cressyl violet. Sometimes, the tissue in a block will 
>> splinter once it hits the blade. Usually all samples from the same 
>> animal splinter but this is not always the case. If I put the block 
>> into the water bath (sectioning surface exposed to water or not) this 
>> seems to stop the splintering for 1-200 um worth of tissue. However, I
> 
>> am afraid this may bring error into the histological analysis of my 
>> tissue.
>> 
>> I assume it has something to do with the protocol I am using to 
>> prepare the tissue. I guessed that maybe the dehydration, alcohol 
>> clearing, or paraffin infiltration are not complete, resulting in the 
>> problem I have. However, I looked at various FFPE protocols and each 
>> of my wash steps are longer, which may be the problem? I was wondering
> 
>> if anyone has encountered this before, or if anyone knows exactly what
> 
>> is going on with my tissue and how I can fix it? Thank you.
>> 
>> Here is my protocol:
>> 
>> -Anesthetize mouse followed by a system flush of 30 ml of PBS and then
> 
>> slow perfusion of 50 ml of 4% PFA.
>> -Brain and spinal cord are removed as a single, in tact unit and 
>> placed into 70% ethanol for 4 hours -80% EtOH for 4 hours -90% EtOH 
>> over night -100% EtOH #1 for 4 hours -100% EtOH #2 for 4 hours -100% 
>> EtOH #3 over night, forebrain cerebellum and spinal cord are separated
> 
>> -Xylene wash #1 for 4 hours -Xylene wash #2 for 4 hours -Xylene wash 
>> #3 over night -Moulton paraffin wash #1 for 4 hours -Moulton paraffin 
>> wash #2 for 4 hours -Moulton paraffin wash #3 over night -Tissue is 
>> embedded and then sectioned into 10 um sections
>> 
>> Again thank you for your time.
>> 
>> Noel W Gray
>> Neuroscience Graduate Program
>> SUNY Upstate Medical University
>> 3219 Weiskotten Hall
>> 766 Irving Ave
>> Syracuse, NY 13210-1630
>> (315) 464-8144
>> grayn@upstate.edu
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
> 
> 

From plucas <@t> biopath.org  Tue Feb 22 10:34:22 2011
From: plucas <@t> biopath.org (Paula Lucas)
Date: Tue Feb 22 10:31:35 2011
Subject: [Histonet] Per Diem needed in Orange County, California
Message-ID: <600A84A135C64EB18E8EFFC0FB36EDDD@biopath.local>

Per Diem histotech needed for vacation coverages and occasional Saturdays.
If interested, please contact me and I'll give you more information.

Thank you,

Paula Lucas

Bio-Path Medical Group

Fountain Valley, CA 92708

714-433-1330

From Nacaela.Johnson <@t> USONCOLOGY.COM  Tue Feb 22 10:33:05 2011
From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela)
Date: Tue Feb 22 10:33:08 2011
Subject: [Histonet] Decal Solution for Bone Marrow Core Biopsies
In-Reply-To: <3ACBB5D73A417547A01970CD3EB5509306FF750F@MAIL1.ecrmc.ci.el-centro.ca.us>
References: <3ACBB5D73A417547A01970CD3EB5509306FF750F@MAIL1.ecrmc.ci.el-centro.ca.us>
Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D553@txhous1eb012.uson.usoncology.int>

We use BBC Rapid-Cal Immuno for 90-120 minutes.  It works great and does not deplete iron stores or affect immunos. 


Thanks,
 
Nacaela Johnson, HTL (ASCP)
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson@USOncology.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Fisher
Sent: Monday, February 21, 2011 1:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Decal Solution for Bone Marrow Core Biopsies

We would like to find another type of decal solution for our bone marrow core biopsies.  The current solution, which is also used on routine bone specimens, is leaching out the iron deposits.  What are others using for BM and times used.  We currently use 45 minutes to one hour after fixation.  These are adult BM cores only.  Thanking you in advance.  

 

M. Fisher

El Centro Regional Medical Center



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Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From MLunetta <@t> luhcares.org Tue Feb 22 10:58:18 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Tue Feb 22 10:58:41 2011 Subject: [Histonet] Medicare Billing Help new rules 2010 Message-ID: <4D6388BA020000A800056BA1@ns.luhcares.org> Hey all, I am having problems finding in the archives the link for the new Medicare pathology rules. Would someone please post it again. I have also tried the Medicare web site and it is not very user friendly. Thanks for the help Matt Lunetta HT (ASCP) From irena.kirbis <@t> hotmail.com Tue Feb 22 11:34:26 2011 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Tue Feb 22 11:34:30 2011 Subject: [Histonet] fixed cytology slides Message-ID: Greetings to all, so far we handled wet-fixed cytology slides as health risk free without gloves, however there is no data regarding this on the net (or at least I wasn't able to find it). I would appreciate response on safety policy in other cytology labs regarding fixed cytology slides and/or any reference on the subject. many thanks Irena Kirbis Institute of Pathology Ljubljana, Slovenia From sgoebel <@t> mirnarx.com Tue Feb 22 12:33:13 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Feb 22 12:33:20 2011 Subject: [Histonet] fixed cytology slides In-Reply-To: References: Message-ID: YES!! Wear gloves!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS Sent: Tuesday, February 22, 2011 11:34 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] fixed cytology slides Greetings to all, so far we handled wet-fixed cytology slides as health risk free without gloves, however there is no data regarding this on the net (or at least I wasn't able to find it). I would appreciate response on safety policy in other cytology labs regarding fixed cytology slides and/or any reference on the subject. many thanks Irena Kirbis Institute of Pathology Ljubljana, Slovenia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wlecorch <@t> rwjuhh.edu Tue Feb 22 12:40:55 2011 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Tue Feb 22 12:41:03 2011 Subject: [Histonet] Histonet Digest, Vol 87, Issue 38 fixed cytology slides Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71D1CC71BED@HAMEXMBA.rwjham.local> Message: 15 Date: Tue, 22 Feb 2011 18:34:26 +0100 From: IRENA SREBOTNIK KIRBIS Subject: [Histonet] fixed cytology slides To: , Message-ID: Content-Type: text/plain; charset="iso-8859-2" Greetings to all, so far we handled wet-fixed cytology slides as health risk free without gloves, however there is no data regarding this on the net (or at least I wasn't able to find it). I would appreciate response on safety policy in other cytology labs regarding fixed cytology slides and/or any reference on the subject. many thanks Irena Kirbis Institute of Pathology Ljubljana, Slovenia You should handle all of your cytology slides with gloves on to prevent epithelial contamination regardless of fixation/health risk Bill Lecorchick Cytology Prep.Tech. 609-584-5128 Fax 609-584-6439 wlecorch@rwjuhh.edu www.rwjhamilton.org From rmweber113 <@t> comcast.net Tue Feb 22 12:47:15 2011 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Tue Feb 22 12:47:18 2011 Subject: [Histonet] Remedial action plan Message-ID: <171147792.1065791.1298400435212.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Hi,? Does anyone have a procedure for a Remedial Action Plan for histology/cytology they are willing to share? Thank you, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC From NMargaryan <@t> childrensmemorial.org Tue Feb 22 13:26:47 2011 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Feb 22 13:29:48 2011 Subject: [Histonet] LCM & archival FFPE tissue Message-ID: Hi all, I have a couple questions I would like to throw out to the experts: 1) 1) Is it possible to extract RNA and DNA for gene expression purpose after usual IHC procedure on 4um sections of archival FFPE breast tumor tissue (If tissue was in citrate buffer, pH 6.0; several blocking steps; one hour in primary Ab (less then one hour will not work); with immunoperoxidase-DAB detection follow the Hematoxylin? 2) Or I need only H&E? 2) 3) Is 4-5 um section good enough? 3) 4) How many sections need to have really good and highest yields? 4) 5) What temperature of Proteinase K is need 42? or 60?C? 5) I've the LCM system from Arcturus and the Pico Pure Kit. Any suggestions? Thanks in advance, Naira From Karen.Kay <@t> albertahealthservices.ca Tue Feb 22 13:35:05 2011 From: Karen.Kay <@t> albertahealthservices.ca (Karen Kay) Date: Tue Feb 22 13:35:16 2011 Subject: [Histonet] ARTISAN STAINER - DAKO - WASTE DISPOSAL Message-ID: <14E77BDEC6B22749B75CAC82EC2090FF025E0048F6@EXMBXC5.crha.bewell.ca> Hello, A question for Dako's Artisan Stainer users as to what you are doing with the Waste disposal and in particular the Trace Metals waste. Are you neutralizing it and if so, could you provide proportions of waste to 0.85% NaCl? We have recently started using the instrument and are trying to develop the waste handling processes from the perpectives of safety of our staff and meeting our regulatory guidelines. Thank you Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital South Zone West - Alberta Health Services 960 - 19 Street South Lethbridge, Alberta, Canada T1J 1W5 Phone: (403) 388-6061 Fax: (403) 388-6067 karen.kay@albertahealthservices.ca ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From JWeems <@t> sjha.org Tue Feb 22 13:38:34 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Feb 22 13:38:38 2011 Subject: [Histonet] RE: ARTISAN STAINER - DAKO - WASTE DISPOSAL In-Reply-To: <14E77BDEC6B22749B75CAC82EC2090FF025E0048F6@EXMBXC5.crha.bewell.ca> References: <14E77BDEC6B22749B75CAC82EC2090FF025E0048F6@EXMBXC5.crha.bewell.ca> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E13D1C3@CHEXCMS10.one.ads.che.org> We have our picked up by a hazardous waste company. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karen Kay Sent: Tuesday, February 22, 2011 14:35 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ARTISAN STAINER - DAKO - WASTE DISPOSAL Hello, A question for Dako's Artisan Stainer users as to what you are doing with the Waste disposal and in particular the Trace Metals waste. Are you neutralizing it and if so, could you provide proportions of waste to 0.85% NaCl? We have recently started using the instrument and are trying to develop the waste handling processes from the perpectives of safety of our staff and meeting our regulatory guidelines. Thank you Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital South Zone West - Alberta Health Services 960 - 19 Street South Lethbridge, Alberta, Canada T1J 1W5 Phone: (403) 388-6061 Fax: (403) 388-6067 karen.kay@albertahealthservices.ca ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From wdesalvo.cac <@t> hotmail.com Tue Feb 22 14:35:23 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Feb 22 14:35:26 2011 Subject: [Histonet] ARTISAN STAINER - DAKO - WASTE DISPOSAL In-Reply-To: <14E77BDEC6B22749B75CAC82EC2090FF025E0048F6@EXMBXC5.crha.bewell.ca> References: <14E77BDEC6B22749B75CAC82EC2090FF025E0048F6@EXMBXC5.crha.bewell.ca> Message-ID: We use Clean Harbors Hazardous Waste Disposal to remove the liquid waste in total. The Artisan's have their own waste stream and container. William DeSalvo, B.S., HTL(ASCP) > From: Karen.Kay@albertahealthservices.ca > To: histonet@lists.utsouthwestern.edu > Date: Tue, 22 Feb 2011 12:35:05 -0700 > Subject: [Histonet] ARTISAN STAINER - DAKO - WASTE DISPOSAL > > Hello, > A question for Dako's Artisan Stainer users as to what you are doing with the Waste disposal and in particular the Trace Metals waste. Are you neutralizing it and if so, could you provide proportions of waste to 0.85% NaCl? > We have recently started using the instrument and are trying to develop the waste handling processes from the perpectives of safety of our staff and meeting our regulatory guidelines. Thank you > > Karen J Kay, MLT > Supervisor - Histopathology and Cytology Laboratory > Chinook Regional Hospital > South Zone West - Alberta Health Services > 960 - 19 Street South > Lethbridge, Alberta, Canada > T1J 1W5 > Phone: (403) 388-6061 > Fax: (403) 388-6067 > karen.kay@albertahealthservices.ca > > > > ________________________________ > This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Feb 22 15:50:11 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Feb 22 15:50:19 2011 Subject: [Histonet] HURRY the GSH meeting deadline for hotel reservations is TOMORROW Message-ID: <9BF995BC0E47744E9673A41486E24EE22DBB4B1042@MERCERMAIL.MercerU.local> Reminder for ALL histotechs, Last day before deadline at the Mountain Creek Inn tomorrow, the 23rd. The actual meeting registration has no deadline, but the prices will go up at the hotel and rooms are going fast. Call today, and definitely by tomorrow. The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge. The deadline for making hotel reservations is tomorrow, Feb 23, 2011 so that gives you a day to make your plans to attend, don't delay. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The complete program can be downloaded from our website at this link: www.histosearch.com/gsh> then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form on page 4. The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance. Come TRANSFORM yourselves. Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From mia.woodruff <@t> qut.edu.au Tue Feb 22 21:32:33 2011 From: mia.woodruff <@t> qut.edu.au (Mia Woodruff) Date: Tue Feb 22 21:32:45 2011 Subject: [Histonet] Sirius red stain - picric acid substitute - water - really? Message-ID: Hello all, I have been undertaking sirius red staining using picric acid, I was under the impression (from papers) that picric acid is an important component of the procedure and read that it prevents non-specific binding of the dye to things other than collagen. However, I have recently found a paper which suggests I can simply use water instead of picric acid, seems quite a long shot but I wondered if anyone has experience with the water technique- given that picric acid is pretty dangerous I would be keen to move away from using it but only if scientifically sound to do so. I don't want to jeopardize my results but if this method works then it's a far safer and cheaper safer approach. Any advice? Paper: A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in Medicine, Volume 9, Number 1, 1998 , pp. 47-51(5) Many thanks Mia From irena.kirbis <@t> hotmail.com Tue Feb 22 23:35:52 2011 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Tue Feb 22 23:36:43 2011 Subject: [Histonet] infectious fixed tissue and cells Message-ID: Dear all, could anyone out there provide a scientific reason for wearing gloves handling slides with FFPE sections and FIXED cytology slides? I could understand that by wearing gloves we could prevent epithelial contamination of cytology slides but I would like to know which microorganism could survive FFPE procedure or cytology fixation and crowl around the slide after that? if cutting of FFPE tissue requires gloves what about archiving them, is it also necessary to wear the gloves? isn't the aerosol during the cutting more dangereous? are FFPE tissues and FIXED cytology slides considered infectious as the fresh tissue/cells? is there any study supporting practice of wearing the gloves or it is just superstition? or perhaps the gloves industry support? many thanks for your thoughts! Irena Kirbis From Melissa.Kuhnla <@t> chsli.org Wed Feb 23 06:36:33 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Feb 23 06:37:20 2011 Subject: [Histonet] alcian blue-PAS control Message-ID: Hello, Can anyone recommend a good control tissue to use for Alcian blue-PAS? Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From Melissa.Kuhnla <@t> chsli.org Wed Feb 23 06:50:23 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Feb 23 06:50:33 2011 Subject: [Histonet] FW: alcian blue-PAS control Message-ID: Can anyone recommend tissue besides duodenum?? _____________________________________________ From: Kuhnla, Melissa Sent: Wednesday, February 23, 2011 7:37 AM To: ' (histonet@lists.utsouthwestern.edu)' Subject: alcian blue-PAS control Hello, Can anyone recommend a good control tissue to use for Alcian blue-PAS? Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From sfonner <@t> labpath.com Wed Feb 23 07:21:33 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Wed Feb 23 07:24:27 2011 Subject: [Histonet] IgG and IgM on mouse kidneys In-Reply-To: References: Message-ID: <000401cbd35c$97181160$c5483420$@com> Liz, You can perform IgG and IgM on FFPE tissue samples. We do them through IHC on our Ventana Ultra and get the antibodies from Cell Marque. Our pathologists are happy with them. Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Saturday, February 19, 2011 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IgG and IgM on mouse kidneys Hello all I have a quick question. Can I perform IgG and IgM staining on mouse kidneys on FFPE tissue samples? I thought that this type of staining is normally completed on frozen sections via Immunofluorescence. Any advice would be helpful Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.byrnes <@t> accelpath.com Wed Feb 23 07:35:22 2011 From: a.byrnes <@t> accelpath.com (Andrew Byrnes) Date: Wed Feb 23 07:35:28 2011 Subject: [Histonet] Digital Pathology/Telepathology Message-ID: <4F28ED3D-62FF-4609-9FDD-D92F3931B4F4@accelpath.com> Dear Histonet, Please don't take this as spam. I am just gathering some information... I am curious to know if any of you are currently working with digital whole slide scanners and what your experience has been to date. Additionally, my company provides telepathology services by putting a digital scanner into your lab allowing our pathology group to access electronic requisitions and digital slides remotely. We then return a report electronically within 24 hours. All is stored on our servers for secure access from anywhere at anytime. The idea is to allow easy access to our fully sub-specialized pathology group by any laboratories that may have a need for more consistent pathology coverage or if there are quality issues. Same concept as teleradiology (Virtual Radiologic, Nighthawk Radiology) which is implemented in many hospitals throughout the US. If interested, our website is www.accelpath.com Would love to hear your thoughts on these subjects... digital path and telepath. Have a great day! Andrew Byrnes AccelPath From akemiat3377 <@t> yahoo.com Wed Feb 23 08:02:57 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Feb 23 08:03:01 2011 Subject: [Histonet] CAP Message-ID: <499302.17900.qm@web113803.mail.gq1.yahoo.com> Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL From MSHERWOOD <@t> PARTNERS.ORG Wed Feb 23 08:04:01 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Feb 23 08:04:07 2011 Subject: [Histonet] Sirius red stain - picric acid substitute - water -really? In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB54A2@PHSXMB30.partners.org> I would do a trial run on "extra" slides or non-valuable tissue? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mia Woodruff Sent: Tuesday, February 22, 2011 10:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sirius red stain - picric acid substitute - water -really? Hello all, I have been undertaking sirius red staining using picric acid, I was under the impression (from papers) that picric acid is an important component of the procedure and read that it prevents non-specific binding of the dye to things other than collagen. However, I have recently found a paper which suggests I can simply use water instead of picric acid, seems quite a long shot but I wondered if anyone has experience with the water technique- given that picric acid is pretty dangerous I would be keen to move away from using it but only if scientifically sound to do so. I don't want to jeopardize my results but if this method works then it's a far safer and cheaper safer approach. Any advice? Paper: A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in Medicine, Volume 9, Number 1, 1998 , pp. 47-51(5) Many thanks Mia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Wanda.Smith <@t> HCAhealthcare.com Wed Feb 23 08:06:44 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Feb 23 08:06:49 2011 Subject: [Histonet] CAP In-Reply-To: <499302.17900.qm@web113803.mail.gq1.yahoo.com> References: <499302.17900.qm@web113803.mail.gq1.yahoo.com> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A04F8E3B@NADCWPMSGCMS03.hca.corpad.net> Akemi, Keep us informed!!! I too, am in my CAP window!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 9:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Feb 23 08:11:31 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Feb 23 08:10:03 2011 Subject: [Histonet] Sirius red stain - picric acid substitute - water -really? In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB54A2@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB54A2@PHSXMB30.partners.org> Message-ID: Since you can buy a saturated Picric acid solution and I have for years why are you worried? It is stable in a saturated liquid and you can purchase fairly small amounts at a time. I only worry if find some in a powder form turning a nasty colour. It has been about twenty five years since I had that happen and immediately went to pre-mixed solutions. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, February 23, 2011 8:04 AM To: Mia Woodruff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sirius red stain - picric acid substitute - water -really? I would do a trial run on "extra" slides or non-valuable tissue? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mia Woodruff Sent: Tuesday, February 22, 2011 10:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sirius red stain - picric acid substitute - water -really? Hello all, I have been undertaking sirius red staining using picric acid, I was under the impression (from papers) that picric acid is an important component of the procedure and read that it prevents non-specific binding of the dye to things other than collagen. However, I have recently found a paper which suggests I can simply use water instead of picric acid, seems quite a long shot but I wondered if anyone has experience with the water technique- given that picric acid is pretty dangerous I would be keen to move away from using it but only if scientifically sound to do so. I don't want to jeopardize my results but if this method works then it's a far safer and cheaper safer approach. Any advice? Paper: A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in Medicine, Volume 9, Number 1, 1998 , pp. 47-51(5) Many thanks Mia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From JEllin <@t> yumaregional.org Wed Feb 23 09:23:43 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Feb 23 09:23:48 2011 Subject: [Histonet] CAP In-Reply-To: <499302.17900.qm@web113803.mail.gq1.yahoo.com> References: <499302.17900.qm@web113803.mail.gq1.yahoo.com> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D692F@EXCHANGECLUSTER.yumaregional.local> Akemi, were you inspected with the new CAP checklist or the old one? The situation in our lab is that we were given the checklist and then they changed us to the new format. Your thoughts -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 7:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From akbitting <@t> geisinger.edu Wed Feb 23 09:28:50 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Feb 23 09:28:56 2011 Subject: [Histonet] CAP In-Reply-To: <499302.17900.qm@web113803.mail.gq1.yahoo.com> References: <499302.17900.qm@web113803.mail.gq1.yahoo.com> Message-ID: <4D64E162.2B7F.00C9.1@geisinger.edu> We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. >>> Akemi Allison 2/23/2011 9:02 AM >>> Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Wanda.Smith <@t> HCAhealthcare.com Wed Feb 23 09:31:56 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Feb 23 09:32:00 2011 Subject: [Histonet] CAP In-Reply-To: <4D64E162.2B7F.00C9.1@geisinger.edu> References: <499302.17900.qm@web113803.mail.gq1.yahoo.com> <4D64E162.2B7F.00C9.1@geisinger.edu> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A0621635@NADCWPMSGCMS03.hca.corpad.net> Sometimes it is a good thing when CAP says you are cramped for space. It "sometimes" makes the hospital Administration wake up and take notice!!! W WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, February 23, 2011 10:29 AM To: histonet; Akemi Allison Subject: Re: [Histonet] CAP We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. >>> Akemi Allison 2/23/2011 9:02 AM >>> Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Wed Feb 23 09:35:08 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Feb 23 09:35:12 2011 Subject: [Histonet] CAP In-Reply-To: <4D64E162.2B7F.00C9.1@geisinger.edu> References: <499302.17900.qm@web113803.mail.gq1.yahoo.com> <4D64E162.2B7F.00C9.1@geisinger.edu> Message-ID: What?!? A histology lab with no enough space...who ever heard of such a thing Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, February 23, 2011 9:29 AM To: histonet; Akemi Allison Subject: Re: [Histonet] CAP We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. >>> Akemi Allison 2/23/2011 9:02 AM >>> Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtaylor <@t> meriter.com Wed Feb 23 09:40:42 2011 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Wed Feb 23 09:41:47 2011 Subject: [Histonet] AMACR (P504S) Message-ID: Hi Everyone, Our pathologists are interested in using this antibody to detect metastatic renal cell carcinoma in paraffin sections. They are not interested in an antibody cocktail, but also want to use it to detect prostate cancer. I'd like to know what company and clone most labs are using. Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From SDrew <@t> uwhealth.org Wed Feb 23 09:52:07 2011 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Wed Feb 23 09:52:13 2011 Subject: [Histonet] RE: [IHCRG] AMACR (P504S) In-Reply-To: References: Message-ID: <738A7878143FF74BB77436E255743C1A0100069A@UWHC-MAIL03.uwhis.hosp.wisc.edu> We currently use Cat#Z2001, clone 13H4 from Zeta Corp with great results, although we only use it for HMWCK/p63/P504s stain. We have also used a predilute (PP200) from BioCare (that worked quite well). Sally ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Taylor, Jean Sent: Wednesday, February 23, 2011 9:41 AM To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com Subject: [IHCRG] AMACR (P504S) Hi Everyone, Our pathologists are interested in using this antibody to detect metastatic renal cell carcinoma in paraffin sections. They are not interested in an antibody cocktail, but also want to use it to detect prostate cancer. I'd like to know what company and clone most labs are using. Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From DKBoyd <@t> chs.net Wed Feb 23 10:01:06 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Feb 23 10:01:25 2011 Subject: [Histonet] CAP In-Reply-To: <4D64E162.2B7F.00C9.1@geisinger.edu> Message-ID: That's a good thing! That means you need more ventilation and admin listens when inspectors speak! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Angela Bitting" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/23/2011 10:30 AM To "histonet" , "Akemi Allison" cc Subject Re: [Histonet] CAP We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. >>> Akemi Allison 2/23/2011 9:02 AM >>> Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From liz <@t> premierlab.com Wed Feb 23 10:48:51 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Feb 23 10:48:55 2011 Subject: [Histonet] Digital Pathology/Telepathology In-Reply-To: <4F28ED3D-62FF-4609-9FDD-D92F3931B4F4@accelpath.com> Message-ID: Andrew We are a research contract lab and have been scanning slides (WSI) since 2007. We have an Aperio Scanscope XT. Our clients have secure remote access to their images. We manage our own server, storage (20TB) and backup locally. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, February 23, 2011 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Digital Pathology/Telepathology Dear Histonet, Please don't take this as spam. I am just gathering some information... I am curious to know if any of you are currently working with digital whole slide scanners and what your experience has been to date. Additionally, my company provides telepathology services by putting a digital scanner into your lab allowing our pathology group to access electronic requisitions and digital slides remotely. We then return a report electronically within 24 hours. All is stored on our servers for secure access from anywhere at anytime. The idea is to allow easy access to our fully sub-specialized pathology group by any laboratories that may have a need for more consistent pathology coverage or if there are quality issues. Same concept as teleradiology (Virtual Radiologic, Nighthawk Radiology) which is implemented in many hospitals throughout the US. If interested, our website is www.accelpath.com Would love to hear your thoughts on these subjects... digital path and telepath. Have a great day! Andrew Byrnes AccelPath _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Feb 23 10:50:35 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Feb 23 10:50:41 2011 Subject: [Histonet] CAP In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8035D692F@EXCHANGECLUSTER.yumaregional.local> References: <499302.17900.qm@web113803.mail.gq1.yahoo.com> <29BE166A2CF48D459853F8EC57CD37E8035D692F@EXCHANGECLUSTER.yumaregional.local> Message-ID: <885248.80684.qm@web113818.mail.gq1.yahoo.com> We were given the new CAP checklist.? I totally revamped our SOP Manuals, ALL QC forms, etc. to comply.? This is the 1st Childrens Hospital CAP inspection I have undergone.? We are inspected by other Children Hospital inspecting teams.? After all the hard work, they did not go through any of our SOP's, QC manuals, Special Stains, validation protocols?manuals for either histology or the IHC lab.? Very odd indeed.? They spoke with the AP manager for our department only, except to ask one of the histology techs how we disposed of our hazardous waste. Stay tuned till after the summation. ?Akemi Allison BS, HT(ASCP)HTL ________________________________ From: Jesus Ellin To: Akemi Allison ; histonet Sent: Wed, February 23, 2011 7:23:43 AM Subject: RE: [Histonet] CAP Akemi, were you inspected with the new CAP checklist or the old one? The situation in our lab is that we were given the checklist and then they changed us to the new format.? Your thoughts -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 7:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window!? We had our CAP inspection yesterday and having our summation this morning at 9:00.? I think our department did pretty good.? Keeping my fingers crossed.? Akemi Allison BS, HT(ASCP)HTL ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From donna_suresch <@t> merck.com Wed Feb 23 10:57:30 2011 From: donna_suresch <@t> merck.com (Suresch, Donna L.) Date: Wed Feb 23 10:57:38 2011 Subject: [Histonet] Re: LCM & archival FFPE tissue (Margaryan, Naira) Message-ID: <66A114FCAC287749A5103A287324A9E202B4C162@USCTMX1151.merck.com> I have used archival FFPE slides that had used antigen retrieval methods for LCM and the yields seemed to be good. The RNA/DNA yields obviously depend on how many cells you are collecting. We routinely used 8um sections for the LCM to increase yields. If you are interested in collecting a population that is very limited in your samples (i.e., ER+ or PR+ in small tumors), you will need to have maybe 20 or more slides (estimate). I would collect those slides into a single microcentrifuge tube (insert LCM cap and invert it to get cells into buffer) using lysing buffer, heat at 42C for 30 min, spin at 2000x g for 1 min, decant buffer, resuspend pellet into RLT/70% ethanol, and freeze the samples at -80 degrees C until ready for PCR. There are some excellent protocols from Quiagen online that will be helpful. Hope this helps. Donna L. Suresch Merck Research Laboratories Research Biologist Imaging Research - West Point Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 <> Message: 4 Date: Tue, 22 Feb 2011 13:26:47 -0600 From: "Margaryan, Naira" Subject: [Histonet] LCM & archival FFPE tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" Hi all, I have a couple questions I would like to throw out to the experts: 1) 1) Is it possible to extract RNA and DNA for gene expression purpose after usual IHC procedure on 4um sections of archival FFPE breast tumor tissue (If tissue was in citrate buffer, pH 6.0; several blocking steps; one hour in primary Ab (less then one hour will not work); with immunoperoxidase-DAB detection follow the Hematoxylin? 2) Or I need only H&E? 2) 3) Is 4-5 um section good enough? 3) 4) How many sections need to have really good and highest yields? 4) 5) What temperature of Proteinase K is need 42?? or 60??C? 5) I've the LCM system from Arcturus and the Pico Pure Kit. Any suggestions? Thanks in advance, Naira Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From trathborne <@t> somerset-healthcare.com Wed Feb 23 09:06:23 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Feb 23 11:06:06 2011 Subject: [Histonet] Bone marrow charges In-Reply-To: <09411E0112A96A459D8D5FBDAB9C15C71D1D05626D@HAMEXMBA.rwjham.local> Message-ID: Are your blocks (1 bx and 1 clot) separate specimens, or just one specimen with 2 blocks? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Goodwin, Diana Sent: Monday, February 21, 2011 11:40 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Bone marrow charges Greetings, Histonetters. How many times can I charge 88313 for a bone marrow case that has an Iron stain on 2 separate blocks (one for the bx and one for the aspirate clot) and also on 1 smear made from the aspirate clot, a PAS on the 2 blocks, a Trichrome and a Retic on the bx. block, and a Wright/ Giemsa on 4 smears? Thank you!!! Diana Goodwin Supervisor, Histology Laboratory RWJUHHNJ Diana Goodwin Supervisor, Histology Laboratory xt. 6996 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From DSiena <@t> statlab.com Wed Feb 23 11:29:57 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed Feb 23 11:30:03 2011 Subject: [Histonet] Diff Quik/Wright stain for stool samples Message-ID: Hello All, Does anyone use a Diff Quik protocol for parasites in stool samples and if so could you send me a copy of your procedure? I would greatly appreciate it. Thank you all. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com From jaylundgren <@t> gmail.com Wed Feb 23 12:09:17 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Feb 23 12:09:34 2011 Subject: [Histonet] CAP In-Reply-To: <885248.80684.qm@web113818.mail.gq1.yahoo.com> References: <499302.17900.qm@web113803.mail.gq1.yahoo.com> <29BE166A2CF48D459853F8EC57CD37E8035D692F@EXCHANGECLUSTER.yumaregional.local> <885248.80684.qm@web113818.mail.gq1.yahoo.com> Message-ID: If your lab smells like oranges, just open your CAP window and air it out. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From histotech <@t> imagesbyhopper.com Wed Feb 23 13:37:09 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Feb 23 13:37:08 2011 Subject: [Histonet] Joint Comm and patient identifiers Message-ID: <00b501cbd391$12c1a3b0$3844eb10$@imagesbyhopper.com> Hi Histonetters! I have a question related to the two patient identifiers that TJC requires: can anyone point (online) me to the actual regulation? It was my understanding that the 2 identifiers related to the *collection* of the specimen, meaning that the container and associated requisition had to have 2 positive patient identifiers. The question is, do they *specifically* state that the 2 identifiers must be carried through to the final surgical slide? The reason I ask is that I have a friend who got dinged for their slides not having 2 patient identifiers on them. They have the surgical number and name of institution, but not the patient name or MRN. My friend is just looking for the actual statute so that he can read and follow exactly as expected. Also, can anyone confirm that the surgical number and a bar code would suffice as 2 identifiers? Thanks! Michelle From cpyse <@t> x-celllab.com Wed Feb 23 13:36:52 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Feb 23 13:38:08 2011 Subject: [Histonet] IHC Equipment Message-ID: <000001cbd391$05b1ad00$11150700$@com> Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com From robin_dean <@t> compbio.com Wed Feb 23 13:43:25 2011 From: robin_dean <@t> compbio.com (Robin Dean) Date: Wed Feb 23 13:46:28 2011 Subject: [Histonet] use of destained sections in IHC? Message-ID: <004b01cbd391$efa7c7a0$cef756e0$@com> Hi all, Is it possible to de-stain H&E stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com From akbitting <@t> geisinger.edu Wed Feb 23 13:50:34 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Feb 23 13:50:44 2011 Subject: [Histonet] CAP In-Reply-To: <885248.80684.qm@web113818.mail.gq1.yahoo.com> References: <499302.17900.qm@web113803.mail.gq1.yahoo.com> <29BE166A2CF48D459853F8EC57CD37E8035D692F@EXCHANGECLUSTER.yumaregional.local> <885248.80684.qm@web113818.mail.gq1.yahoo.com> Message-ID: <4D651EBA.2B7F.00C9.1@geisinger.edu> I did notice CAP inspectors concentrated more on safety this time around. >>> Akemi Allison 2/23/2011 11:50 AM >>> We were given the new CAP checklist. I totally revamped our SOP Manuals, ALL QC forms, etc. to comply. This is the 1st Childrens Hospital CAP inspection I have undergone. We are inspected by other Children Hospital inspecting teams. After all the hard work, they did not go through any of our SOP's, QC manuals, Special Stains, validation protocols manuals for either histology or the IHC lab. Very odd indeed. They spoke with the AP manager for our department only, except to ask one of the histology techs how we disposed of our hazardous waste. Stay tuned till after the summation. Akemi Allison BS, HT(ASCP)HTL ________________________________ From: Jesus Ellin To: Akemi Allison ; histonet Sent: Wed, February 23, 2011 7:23:43 AM Subject: RE: [Histonet] CAP Akemi, were you inspected with the new CAP checklist or the old one? The situation in our lab is that we were given the checklist and then they changed us to the new format. Your thoughts -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 7:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From DKBoyd <@t> chs.net Wed Feb 23 13:54:17 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Feb 23 13:54:25 2011 Subject: [Histonet] Joint Comm and patient identifiers In-Reply-To: <00b501cbd391$12c1a3b0$3844eb10$@imagesbyhopper.com> Message-ID: The Joint Commission web site: http://www.jointcommission.org/accreditation/accreditation_main.aspx click on Standards and then click on National Patient Safety Goals. It is the first standard in the chapter. Standard number: NPSG.01.01.01 Debbie M. Boyd, HT(ASCP) l Chief Hitologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Sent by: histonet-bounces@lists.utsouthwestern.edu 02/23/2011 02:40 PM To "'histonet'" cc Subject [Histonet] Joint Comm and patient identifiers Hi Histonetters! I have a question related to the two patient identifiers that TJC requires: can anyone point (online) me to the actual regulation? It was my understanding that the 2 identifiers related to the *collection* of the specimen, meaning that the container and associated requisition had to have 2 positive patient identifiers. The question is, do they *specifically* state that the 2 identifiers must be carried through to the final surgical slide? The reason I ask is that I have a friend who got dinged for their slides not having 2 patient identifiers on them. They have the surgical number and name of institution, but not the patient name or MRN. My friend is just looking for the actual statute so that he can read and follow exactly as expected. Also, can anyone confirm that the surgical number and a bar code would suffice as 2 identifiers? Thanks! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From LSebree <@t> uwhealth.org Wed Feb 23 13:59:38 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Feb 23 13:59:42 2011 Subject: [Histonet] use of destained sections in IHC? In-Reply-To: <004b01cbd391$efa7c7a0$cef756e0$@com> References: <004b01cbd391$efa7c7a0$cef756e0$@com> Message-ID: <8C023B4AB999614BA4791BAEB26E273839A1FB@UWHC-MAIL01.uwhis.hosp.wisc.edu> Robin, We just take the coverslip/film off, hydrate to water and run for IHC BUT many times the H&E slide is not a "+" slide so we end up losing tissue. It is not necessary to destain the H&E first. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Dean Sent: Wednesday, February 23, 2011 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] use of destained sections in IHC? Hi all, Is it possible to de-stain H&E stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Wed Feb 23 14:00:16 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Feb 23 14:01:21 2011 Subject: [Histonet] use of destained sections in IHC? In-Reply-To: <004b01cbd391$efa7c7a0$cef756e0$@com> References: <004b01cbd391$efa7c7a0$cef756e0$@com> Message-ID: <0B8979A204680A42B93A52B486088CD912AE750363@CUAEXH1.GCU-MD.local> It will work and if you are performing antigen retrieval then you won't need to decolorize the slide, just rehydrate and perform per you usual protocol. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Dean [robin_dean@compbio.com] Sent: Wednesday, February 23, 2011 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] use of destained sections in IHC? Hi all, Is it possible to de-stain H&E stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From sgoebel <@t> mirnarx.com Wed Feb 23 14:01:48 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Feb 23 14:01:52 2011 Subject: [Histonet] RRAS Message-ID: So...trying to find someone who sells RRAS antibody that isn't Abcam. I can't seem to make the Abcam antibody work and I have been asked to try and get it from a different company. 2. Does anyone have any experience with RRAS? I have tried dilutions from 1:500 all the way up to 1:2000. I keep getting a blush endogenous stain that I can't seem to get out even after blocking for everything under the sun. I am using a normal pH antigen retrieval (DAKO). Should I try high pH? I don't know if that will make the positive staining come out, I think it will make it worse? Thanks histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From liz <@t> premierlab.com Wed Feb 23 14:12:17 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Feb 23 14:12:20 2011 Subject: [Histonet] IHC Equipment In-Reply-To: <000001cbd391$05b1ad00$11150700$@com> Message-ID: Cindy We have three dako autostainers and we really like them a lot. We are a contract research lab so flexibility and system openness is key for us. The only other instrument that I have demoed is the Leica Bond, but unless I purchased the research software (which is a bit expensive) I was tied into using at least one of their reagents and their reagents are purchased in kits, so for my lab it was not a good fit financially since I already had two dako stainers on reagent rental. If you have the space I would demo the different instruments on the market, see which one works best for you. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader <@t> mcgill.ca Wed Feb 23 14:57:08 2011 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Wed Feb 23 14:57:55 2011 Subject: [Histonet] Short term tissue storage PBS??? Message-ID: <76D119EF12C904418800ED67CCB2062929A1F1E5A0@EXMBXVS1.campus.mcgill.ca> Hi All, It has come to my attention recently that some of our histology clients are submitting their samples in PBS after fixation, instead of 70% alcohol. When I ask them why they don't know and say they have been told to. I have always followed the procedure that after fixation specimens are stored, short term in 70% alcohol, until you are ready to process them. Has something changed lately that I am not aware of? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From rjbuesa <@t> yahoo.com Wed Feb 23 15:08:47 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 23 15:08:51 2011 Subject: [Histonet] Short term tissue storage PBS??? In-Reply-To: <76D119EF12C904418800ED67CCB2062929A1F1E5A0@EXMBXVS1.campus.mcgill.ca> Message-ID: <320945.78753.qm@web65712.mail.ac4.yahoo.com> Nothing has changed, it is just "one of those things" people do because of fundamental ignorance. PBS is not a preservative and could even favor bacterial growth (depending of the time elapsed). After proper fixation, tissues should be placed in EthOL 70% as a general practice.

Ren? J. ? --- On Wed, 2/23/11, Jo-Ann Bader, Ms. wrote: From: Jo-Ann Bader, Ms. Subject: [Histonet] Short term tissue storage PBS??? To: "Histonet@lists.utsouthwestern.edu" Date: Wednesday, February 23, 2011, 3:57 PM Hi All, It has come to my attention recently that some of our histology clients are submitting their samples in PBS after fixation, instead of 70% alcohol.? When I ask them why they don't know and say they have been told to.? I have always? followed the procedure that after fixation specimens are stored, short term in 70% alcohol, until you are ready to process them. Has something changed lately that I am not aware of? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGleiberman <@t> cbiolabs.com Wed Feb 23 15:39:49 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Wed Feb 23 15:39:55 2011 Subject: [Histonet] RE: Short term tissue storage PBS??? In-Reply-To: <76D119EF12C904418800ED67CCB2062929A1F1E5A0@EXMBXVS1.campus.mcgill.ca> References: <76D119EF12C904418800ED67CCB2062929A1F1E5A0@EXMBXVS1.campus.mcgill.ca> Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C111236@cbiolabs05.CBiolabs.local> Jo-Ann, Nothing is changed, but storage in PBS does not affect neither morphology nor antigens. I used to keep some neutral formalin- or formaldehyde-PBS fixed specimens up to 1 year in sterile PBS at +4 C without any visible changes. So - don't worry. It will be fine. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Wednesday, February 23, 2011 3:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Short term tissue storage PBS??? Hi All, It has come to my attention recently that some of our histology clients are submitting their samples in PBS after fixation, instead of 70% alcohol. When I ask them why they don't know and say they have been told to. I have always followed the procedure that after fixation specimens are stored, short term in 70% alcohol, until you are ready to process them. Has something changed lately that I am not aware of? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From AnthonyH <@t> chw.edu.au Wed Feb 23 15:54:01 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Feb 23 15:54:17 2011 Subject: [Histonet] RE: alcian blue-PAS control In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71571882D9F5@xmdb02.nch.kids> Melissa, We use a multi-tissue control. Our control block contains appendix, umbilical cord and liver (for diastase). The optimum control (if you can get it) would be an intestinal metaplasia of the stomach and combine this with a mucin-producing mesothelioma. To you all, any further ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, 23 February 2011 11:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue-PAS control Hello, Can anyone recommend a good control tissue to use for Alcian blue-PAS? Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Rcartun <@t> harthosp.org Wed Feb 23 15:58:58 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Feb 23 15:59:06 2011 Subject: [Histonet] use of destained sections in IHC? In-Reply-To: <004b01cbd391$efa7c7a0$cef756e0$@com> References: <004b01cbd391$efa7c7a0$cef756e0$@com> Message-ID: <4D653CD1.7400.0077.1@harthosp.org> Yes, you can use H&E-stained slides for IHC; however, if your lesion/tumor is negative and there is no internal positive control, then you may be dealing with a false negative result. Having said that, we have had good luck with the majority of proteins we have tested. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Robin Dean" 2/23/2011 2:43 PM >>> Hi all, Is it possible to de-stain H&E stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Feb 23 16:14:47 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Feb 23 16:14:53 2011 Subject: [Histonet] RE: alcian blue-PAS control In-Reply-To: <6D6BD1DE8A5571489398B392A38A71571882D9F5@xmdb02.nch.kids> Message-ID: Cervix (with both endocervix and ectocervix) contains both glycogen (ecto) and mucin (endo). Jennifer Tony Henwood Sent by: histonet-bounces@lists.utsouthwestern.edu 02/23/2011 01:56 PM To "'Kuhnla, Melissa'" , "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] RE: alcian blue-PAS control Melissa, We use a multi-tissue control. Our control block contains appendix, umbilical cord and liver (for diastase). The optimum control (if you can get it) would be an intestinal metaplasia of the stomach and combine this with a mucin-producing mesothelioma. To you all, any further ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, 23 February 2011 11:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue-PAS control Hello, Can anyone recommend a good control tissue to use for Alcian blue-PAS? Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Feb 23 17:07:37 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Feb 23 17:07:56 2011 Subject: [Histonet] use of destained sections in IHC? In-Reply-To: <0B8979A204680A42B93A52B486088CD912AE750363@CUAEXH1.GCU-MD.local> References: <004b01cbd391$efa7c7a0$cef756e0$@com> <0B8979A204680A42B93A52B486088CD912AE750363@CUAEXH1.GCU-MD.local> Message-ID: <803C28340FE247A5BD19E83687612ECD@JoePC> I wouldn't decolorize the slides. Just remove the coverslip and hydrate to water. Any antigen retrieval should take out the Eosin. You're going to counterstain in Hematoxylin any way. Years ago (ok, many moons ago) I did an informal study. I took 10 antibodies, stained two sets of controls H & E. One set, I used 1% acid-alcohol to decolorize the slides, the other set I just rehydrated to water. The slides that were placed in acid-alcohol either had no staining or a marked decrease in intensity. Joe ----- Original Message ----- From: "Walter Benton" To: "Robin Dean" ; Sent: Wednesday, February 23, 2011 2:00 PM Subject: RE: [Histonet] use of destained sections in IHC? It will work and if you are performing antigen retrieval then you won't need to decolorize the slide, just rehydrate and perform per you usual protocol. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Dean [robin_dean@compbio.com] Sent: Wednesday, February 23, 2011 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] use of destained sections in IHC? Hi all, Is it possible to de-stain H&E stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raestask <@t> grics.net Wed Feb 23 19:19:10 2011 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Wed Feb 23 19:19:18 2011 Subject: [Histonet] Competancies for handling hazardous material Message-ID: <000601cbd3c0$d7ebaf80$87c30e80$@grics.net> Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz From amosbrooks <@t> gmail.com Wed Feb 23 19:56:18 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Feb 23 19:56:21 2011 Subject: [Histonet] Sirius red stain - picric acid substitute - water -really? Message-ID: Hi Mia, In response to your question about the need for picric acid in the sirus red solution. Yes, you do need it. The article you listed was working with collagen gels and not actually tissue sections. If you omit the picric acid you not only loose the yellow counter stain as mentioned in the article, but the sirius red dye binds to other tissue components. It is also critical that the pH of the solution be between 2.0 and 2.3 (picric acid or not) in order for the staining to be specific to these collagen types. If you decide to try this please do so in parallel with the established picric acid technique and certainly make sure that the water is buffered to the necessary pH. I have a great article back in the lab about the stain and how it differentially stains the collagen types and bifringes to further differentiate them. It's a bit old, but definitely still relevant. Now I know there are some folks out there that will inevitably whine that picric acid is a hazardous chemical and shouldn't be used in the lab. Well guys it's a lab, that's just the way it goes. As long as you are conscious of the hazards involved, store, use and dispose of it correctly it can easily be used relatively safely. We all use gas in our car's gas tanks, and that is an extremely hazardous chemical. And some of us travel 75 MPH on the highway while texting and eating McDonalds with our kids fighting in the back seat. Being on the road with those folks is scarier than a bit of properly used picric acid. Just as you watch for that swerving minivan and use your car carefully, respect and use chemicals carefully and everything will be just fine. Amos Brooks On Wed, Feb 23, 2011 at 10:52 AM, wrote: > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mia > Woodruff > Sent: Tuesday, February 22, 2011 10:33 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sirius red stain - picric acid substitute - water > -really? > > Hello all, > > I have been undertaking sirius red staining using picric acid, I was under > the > impression (from papers) that picric acid is an important component of the > procedure and read that it prevents non-specific binding of the dye to > things > other than collagen. However, I have recently found a paper which suggests > I > can simply use water instead of picric acid, seems quite a long shot but I > wondered if anyone has experience with the water technique- given that > picric > acid is pretty dangerous I would be keen to move away from using it but > only if > scientifically sound to do so. I don't want to jeopardize my results but if > this > method works then it's a far safer and cheaper safer approach. Any advice? > > Paper: A specific quantitative assay for collagen synthesis by cells seeded > in > collagen-based biomaterials using sirius red F3B precipitation > LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in > Medicine, Volume 9, Number > 1, > 1998 , pp. 47-51(5) > > > Many thanks > Mia > From macveigh <@t> usc.edu Wed Feb 23 20:29:46 2011 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Wed Feb 23 20:29:50 2011 Subject: [Histonet] Sirius red stain - picric acid substitute Message-ID: <00f301cbd3ca$b3d3d550$1b7b7ff0$@usc.edu> Hi Mia, I tried few different sources of Picric acid. Unfortunately, not all of them worked. The one that is working is from Rowley Biochemical Institute - www.rowleybio.com . I got a small bottle to try - 4 oz. Cat # SO-128. You can probably find it from one of the large vendors. Just a helpful thought Michelle USC Keck School of Medicine From liz <@t> premierlab.com Wed Feb 23 20:51:33 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Feb 23 20:51:38 2011 Subject: [Histonet] Competancies for handling hazardous material References: <000601cbd3c0$d7ebaf80$87c30e80$@grics.net> Message-ID: Rae We have, you need standard training, but the individuals who inspected our lab did not mention compentances. But our inspection happened because we needed to switch from a conditionally exempt small quantity generator to a small quantitiy generator and we then needed to register with the state, etc. They primarily focused on our waste streams and what chemicals were in the lab. I'm not at work right now, but I'll check tomorrow to see if I have anything that I can share with you. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rae Staskiewicz Sent: Wed 2/23/2011 6:19 PM To: Histonet Subject: [Histonet] Competancies for handling hazardous material Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amitapandey <@t> torrentpharma.com Thu Feb 24 04:11:53 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Thu Feb 24 04:16:24 2011 Subject: [Histonet] Polymer detection kit on rat tissue In-Reply-To: <738A7878143FF74BB77436E255743C1A0100069A@UWHC-MAIL03.uwhis.hosp.wisc.edu> References: <738A7878143FF74BB77436E255743C1A0100069A@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: Dear histonetters, I am lost in data sheets of IHC detection kits . Please help me to finalize one good detection kit for my purpose. I am planning to do immunohistochemistry on paraffin embedded rat kidney tissues for mouse monoclonal and rabbit polyclonal antibody (not double labelling). I am looking on Dako envision, vector immpress, Novolink , the company like Biocare/ Max bio vision suggest the Rat specific detection kit....Got confused... Also send me your opinion on ABC detection versus polymer detection system. Thanks in advance for your view. Amita From histotech <@t> imagesbyhopper.com Thu Feb 24 07:54:58 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Feb 24 07:55:27 2011 Subject: [Histonet] Bone marrow charges In-Reply-To: References: Message-ID: <7721BCDE-500E-4046-9D18-F84DEE3C677D@imagesbyhopper.com> It is my understanding that you may now charge for every stain, even if it's two blocks from one "bottle". The CMS requirements changed in Oct of 2009, if memory serves correctly. Perhaps some one else can site the reference or go back into the archives for it. I'm not at my computer, or I would do it myself! Sent from my iPhone On Feb 23, 2011, at 10:06 AM, "Rathborne, Toni" wrote: > Are your blocks (1 bx and 1 clot) separate specimens, or just one specimen with 2 blocks? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Goodwin, > Diana > Sent: Monday, February 21, 2011 11:40 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Bone marrow charges > > > Greetings, Histonetters. > > How many times can I charge 88313 for a bone marrow case that has an Iron stain on 2 separate blocks (one for the bx and one for the aspirate clot) and also on 1 smear made from the aspirate clot, a PAS on the 2 blocks, a Trichrome and a Retic on the bx. block, and a Wright/ Giemsa on 4 smears? > > Thank you!!! > Diana Goodwin > Supervisor, Histology Laboratory > RWJUHHNJ > > > Diana Goodwin > Supervisor, Histology Laboratory > xt. 6996 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Thu Feb 24 08:07:42 2011 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Feb 24 08:07:58 2011 Subject: [Histonet] Joint Comm and patient identifiers In-Reply-To: <00b501cbd391$12c1a3b0$3844eb10$@imagesbyhopper.com> References: <00b501cbd391$12c1a3b0$3844eb10$@imagesbyhopper.com> Message-ID: <00de01cbd42c$35c4a380$a14dea80$@pathview.com> Here's another view on the matter: We have a client who purposely had us remove both the patient name and the MRN from the slide label. In their opinion, it was a violation of patient confidentiality when and if a slide left their organization for consultation or perhaps for further workups. Obviously, not all slides are sent out of the organization, but they took the attitude of 'being safe than sorry' I suspect. Hence, the two identifiers on their slide are the 2 D barcode and the case number. To the best of my knowledge they are TJC inspected and they have never had any issues. Keep in mind that I have not read the regulations, and I come at a lot of things from an IT perspective, but there's a big difference to me in the terminology, 'patient identifiers', and 'human readable patient identifiers'. In the end, this may be an example of people and technology not quite being in synch. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Wednesday, February 23, 2011 11:37 AM To: 'histonet' Subject: [Histonet] Joint Comm and patient identifiers Hi Histonetters! I have a question related to the two patient identifiers that TJC requires: can anyone point (online) me to the actual regulation? It was my understanding that the 2 identifiers related to the *collection* of the specimen, meaning that the container and associated requisition had to have 2 positive patient identifiers. The question is, do they *specifically* state that the 2 identifiers must be carried through to the final surgical slide? The reason I ask is that I have a friend who got dinged for their slides not having 2 patient identifiers on them. They have the surgical number and name of institution, but not the patient name or MRN. My friend is just looking for the actual statute so that he can read and follow exactly as expected. Also, can anyone confirm that the surgical number and a bar code would suffice as 2 identifiers? Thanks! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lea.Alminde <@t> tuhs.temple.edu Thu Feb 24 08:27:16 2011 From: Lea.Alminde <@t> tuhs.temple.edu (Alminde, Lea S) Date: Thu Feb 24 08:27:27 2011 Subject: [Histonet] CAP In-Reply-To: <4D651EBA.2B7F.00C9.1@geisinger.edu> Message-ID: How are you all responding to the Document Control Question and do you have a sample you can share? Thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, February 23, 2011 2:51 PM To: histonet; Akemi Allison; Jesus Ellin Subject: Re: [Histonet] CAP I did notice CAP inspectors concentrated more on safety this time around. >>> Akemi Allison 2/23/2011 11:50 AM >>> We were given the new CAP checklist. I totally revamped our SOP Manuals, ALL QC forms, etc. to comply. This is the 1st Childrens Hospital CAP inspection I have undergone. We are inspected by other Children Hospital inspecting teams. After all the hard work, they did not go through any of our SOP's, QC manuals, Special Stains, validation protocols manuals for either histology or the IHC lab. Very odd indeed. They spoke with the AP manager for our department only, except to ask one of the histology techs how we disposed of our hazardous waste. Stay tuned till after the summation. Akemi Allison BS, HT(ASCP)HTL ________________________________ From: Jesus Ellin To: Akemi Allison ; histonet Sent: Wed, February 23, 2011 7:23:43 AM Subject: RE: [Histonet] CAP Akemi, were you inspected with the new CAP checklist or the old one? The situation in our lab is that we were given the checklist and then they changed us to the new format. Your thoughts -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 7:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message is intended to be for the use of the named recipient, and may contain information that is confidential or privileged. This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Pennsylvania Laws. You can direct questions concerning PHI or HIPAA to the Corporate Compliance and Privacy Officer at (215) 707-5605. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete and destroy all copies of this message. From sgoebel <@t> mirnarx.com Thu Feb 24 08:46:58 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Feb 24 08:47:04 2011 Subject: [Histonet] Polymer detection kit on rat tissue In-Reply-To: References: <738A7878143FF74BB77436E255743C1A0100069A@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: I personally love polymers!! The DAKO envision is actually using polymers. You don't have to buy the kit. Biocare has a ton of awesome polymers for sale that I use in tangent with DAKO. If you want more specifics on my protocols let me know. Good Luck Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amitapandey@torrentpharma.com Sent: Thursday, February 24, 2011 4:12 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Polymer detection kit on rat tissue Dear histonetters, I am lost in data sheets of IHC detection kits . Please help me to finalize one good detection kit for my purpose. I am planning to do immunohistochemistry on paraffin embedded rat kidney tissues for mouse monoclonal and rabbit polyclonal antibody (not double labelling). I am looking on Dako envision, vector immpress, Novolink , the company like Biocare/ Max bio vision suggest the Rat specific detection kit....Got confused... Also send me your opinion on ABC detection versus polymer detection system. Thanks in advance for your view. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Feb 24 09:12:36 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Feb 24 09:12:40 2011 Subject: [Histonet] Polymer detection kit on rat tissue References: <738A7878143FF74BB77436E255743C1A0100069A@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: Anita Since you are working on rat tissue, you have to be careful on what detection systems you use. You will need two different detection systems for your work. One for the mouse monoclonal antibodies and one for the rabbit polyclonal. If you want to use a polymer based detection system then here are some basic rules you will need to follow. 1. For blocking its probably easiest to use a serum free protein block - many vendors have them. 2. For the rabbit polyclonal antibody you can use any vendors anti-rabbit polymer, but you can NOT purchase a dual link polymer (one that will detect both mouse and rabbit primary antibodies) that will lead to background staining 2. For the mouse antibody on rat tissue if you are dead set on using a polymer then you would need to purchase one that is specifically designed to be used for mouse antibodies on rat tissue. Biocare Medical and MaxVision have them, there may be others out there these are the two that we have used in our lab. 3. Polymer based reagents are nice but they are expensive. I don't want you to get the empression that I'm knocking polymer based reagents, we use them in our lab all of the time. But there are other methods or detection systmes available for use on animal tissues if you are on a budget. The key to good success with animal detection systems if you are going to use a secondary antibody rather than a polymer is to make sure that the secondary antibody that you purchase has been cross absorbed to the species you are working with. You must also check the spec sheets they will normally give you a cross reactivity list of tissues that have been tested. Good Luck Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of amitapandey@torrentpharma.com Sent: Thu 2/24/2011 3:11 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Polymer detection kit on rat tissue Dear histonetters, I am lost in data sheets of IHC detection kits . Please help me to finalize one good detection kit for my purpose. I am planning to do immunohistochemistry on paraffin embedded rat kidney tissues for mouse monoclonal and rabbit polyclonal antibody (not double labelling). I am looking on Dako envision, vector immpress, Novolink , the company like Biocare/ Max bio vision suggest the Rat specific detection kit....Got confused... Also send me your opinion on ABC detection versus polymer detection system. Thanks in advance for your view. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dolores_Fischer <@t> baxter.com Thu Feb 24 09:26:35 2011 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Thu Feb 24 09:26:45 2011 Subject: [Histonet] Polymer detection kit on rat tissue In-Reply-To: References: <738A7878143FF74BB77436E255743C1A0100069A@UWHC-MAIL03.uwhis.hosp.wisc.edu> Message-ID: Amita, I know there are others out there with much more immuno experience than myself, but I have been working up procedures on animal tissue for a few years now, so I can give you my opinion. I have always had good results with Biocare products. I would try their polymer kit. Vector impress is also a good option. ABC versus polymer? Polymers are more expensive, but the protocols are shorter and easier. However I always seem to work up new protocols using the ABC method. More troubleshooting options if your procedure doesn't work or you need to optimize the protocol. I have also heard or read that not all antibodies are detected by polymers (comments from the more experienced immuno crowd?), so I start out with ABC and sometimes purchase a polymer kit later. Depends on your budget. Dolores -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amitapandey@torrentpharma.com Sent: Thursday, February 24, 2011 4:12 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Polymer detection kit on rat tissue Dear histonetters, I am lost in data sheets of IHC detection kits . Please help me to finalize one good detection kit for my purpose. I am planning to do immunohistochemistry on paraffin embedded rat kidney tissues for mouse monoclonal and rabbit polyclonal antibody (not double labelling). I am looking on Dako envision, vector immpress, Novolink , the company like Biocare/ Max bio vision suggest the Rat specific detection kit....Got confused... Also send me your opinion on ABC detection versus polymer detection system. Thanks in advance for your view. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From cls71877 <@t> sbcglobal.net Thu Feb 24 10:46:07 2011 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Thu Feb 24 10:46:11 2011 Subject: [Histonet] Other physicians ordering tests Message-ID: <171376.19716.qm@web81202.mail.mud.yahoo.com> Hello Histoland, I have searched the archives with no luck for some guidance concerning outside physicians ordering tests and how others handle this.? We are a physician owned lab specializing in GI.? More and more frequently we are getting requests from outside offices requesting further testing.? Most of these tests are genetic tests in nature and we do not perform them in-house.? Also, we only send consults to reference labs that do third party billing.? This flow has worked fine for us internally, but the external orders are becoming increasingly problematic.? It seems that many insurances specifically exclude genetic testing.? Who notifies the patient then of the potential out of pocket expense?? Does anyone else out there experiencee these cases?? How do you handle it?? My current thought process is to send the block or slide to the requesting physician's office and let them send out for any tests they want, but?I am concerned that will only create confusion in their offices and ultimately delay appropriate patient care.? Any suggestions? Thank you, Cristi From relia1 <@t> earthlink.net Thu Feb 24 10:46:31 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Feb 24 10:46:37 2011 Subject: [Histonet] RELIA Hot Management Opportunity. Histology Lab Manager - Long Island, NY Message-ID: Hi Histonetters!! I have just been engaged to assist in recruiting for this exciting opportunity and I wanted to tell you about it. I am working with a leading pathology laboratory located on Long Island that is in need of a histology lab manager. This person will be responsible for the day shift people, the afternoon, evening, IHC, EM, Molecular/FISH supervisors. The staff count for these areas is in the neighborhood of 125 to 135 staff at this point in time. NYS license, ASCP certification and a minimum of 5 years of supervisory experience in a histology environment to include the technical and administrative responsibilities. IIf you or anyone you know might like more information please contact me, Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. REMEMBER I offer a $500.00 referral bonus if I place someone you refer to me. Enjoy your day! Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From abeharry798 <@t> gmail.com Thu Feb 24 10:55:37 2011 From: abeharry798 <@t> gmail.com (Andrea) Date: Thu Feb 24 10:55:38 2011 Subject: [Histonet] P21 stain Message-ID: Hi everyone, Does anyone know where I can find P21 antibody? Thanks, Andrea Beharry From wbenton <@t> cua.md Thu Feb 24 11:11:11 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Feb 24 11:11:39 2011 Subject: [Histonet] P21 stain In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD9136B4D5D98@CUAEXH1.GCU-MD.local> Cell Marque and it has IVD status. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea [abeharry798@gmail.com] Sent: Thursday, February 24, 2011 11:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] P21 stain Hi everyone, Does anyone know where I can find P21 antibody? Thanks, Andrea Beharry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From kaylee <@t> medwrench.com Thu Feb 24 11:27:07 2011 From: kaylee <@t> medwrench.com (Kaylee McCaffrey) Date: Thu Feb 24 11:27:15 2011 Subject: [Histonet] Hema tek 2000 slide stainer Message-ID: Can anyone help with the below question, originally asked on MedWrench. My bayer hema tek 2000 slide stainer is giving me, sometimes, light staining and suddenly, some times, dark staining. What could be the problem? Link to question: http://www.medwrench.com/?home.readPosts&pagepostNo=2816 Kaylee McCaffrey MedWrench Product Manager From silvinamolinuevo <@t> yahoo.com.ar Thu Feb 24 11:45:02 2011 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Thu Feb 24 11:45:05 2011 Subject: [Histonet] Histonet] Sirius red stain - picric acid substitute Message-ID: <211653.95251.qm@web113617.mail.gq1.yahoo.com> hi mia! yes, you do need picric acid. the function of it is to solubilize all low molecular weight proteins that would interefe with the sirius red stain. this old collagen stain function very well and it is selective for collagen when you use bouin fixative. if not, it is not selective. then if you use polarized light to observe the specimen you can differentiate between various types of collagen (e.g. type I, IV, etc). picric acid would be a hazardous chemical only if you expose directly to flame. we have stored large quantities dry for more than 50 years until everybody becomes crazy, i do not why, and we have no problem with it. i think Amos pointed out a good criteria for hazardous chemicals. may be he is as old as i am..... best wishes, sil From cls71877 <@t> sbcglobal.net Thu Feb 24 11:57:41 2011 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Thu Feb 24 11:57:44 2011 Subject: [Histonet] Thank you! Message-ID: <420223.33451.qm@web81206.mail.mud.yahoo.com> Hello again, Wow!? What a wonderful world it would be if everyone were as quick and helpful as histoland.? Thank you all for the responses to my inquiry about outside physicians ordering testing, this information is very helpful and?I am excited to implement all of your ideas! Sincerely, Cristi From trathborne <@t> somerset-healthcare.com Thu Feb 24 12:00:50 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Feb 24 12:04:50 2011 Subject: [Histonet] Thank you! In-Reply-To: <420223.33451.qm@web81206.mail.mud.yahoo.com> Message-ID: Could you please share this information with the rest of us? The responses must not have gone back out to Histonet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cristi stephenson Sent: Thursday, February 24, 2011 12:58 PM To: Histo Net Subject: [Histonet] Thank you! Hello again, Wow!? What a wonderful world it would be if everyone were as quick and helpful as histoland.? Thank you all for the responses to my inquiry about outside physicians ordering testing, this information is very helpful and?I am excited to implement all of your ideas! Sincerely, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From jclark <@t> pcnm.com Thu Feb 24 12:09:55 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Thu Feb 24 12:10:01 2011 Subject: [Histonet] Prostate needle core protocols Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C014ABE1D@mail.pcnm.com> Hi all, my doc's want to know what protocol others use for cutting needle core biopsies of prostate. We cut a serial ribbon pick up one for H&E and the rest are kept as unstained slides, than trim in about 16-20 microns and take another serial section same as above and than repeat a third time. How do other labs cut their prostates biopsies? Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico From scorpionrider <@t> cox.net Thu Feb 24 12:14:23 2011 From: scorpionrider <@t> cox.net (Mark Turner) Date: Thu Feb 24 12:14:27 2011 Subject: [Histonet] (no subject) Message-ID: <20110224131423.U3P3U.1384195.imail@fed1rmwml45> Several of you have asked that I share our protocol for the P16 on the Ventana XT. I apologize for the delay, but here is what we have worked up and it performs well in our lab: CC1 Standard Antibody incubation @ 37 degrees C. for 28 minutes Amplification Hematoxylin counterstain 4 minutes Bluing Reagent 4 minutes We place the commercial antibody from the CINtec kit in a prep kit and use it as an RTU. Mark A. Turner, Ph.D. HT (ASCP) QIHC | Test Development Team Lead | Department of Laboratory Medicine and Pathology | 480-301-8026 | Fax: 480-301-8372 | turner.mark@mayo.edu Mayo Clinic in Arizona | 13400 E. Shea Blvd. | Scottsdale, AZ 85259 | www.mayoclinic.org From LSebree <@t> uwhealth.org Thu Feb 24 12:20:05 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Feb 24 12:20:10 2011 Subject: [Histonet] p16 protocol for VMS instruments In-Reply-To: <20110224131423.U3P3U.1384195.imail@fed1rmwml45> References: <20110224131423.U3P3U.1384195.imail@fed1rmwml45> Message-ID: <8C023B4AB999614BA4791BAEB26E273801002900@UWHC-MAIL01.uwhis.hosp.wisc.edu> For another method that works on Ventana XTs and BenchMarks: BD antibody (cat.#: 550834), 1:10, mild CC1, 32 " incubation @ 42 degrees, Hem II/8" Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Thursday, February 24, 2011 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Several of you have asked that I share our protocol for the P16 on the Ventana XT. I apologize for the delay, but here is what we have worked up and it performs well in our lab: CC1 Standard Antibody incubation @ 37 degrees C. for 28 minutes Amplification Hematoxylin counterstain 4 minutes Bluing Reagent 4 minutes We place the commercial antibody from the CINtec kit in a prep kit and use it as an RTU. Mark A. Turner, Ph.D. HT (ASCP) QIHC | Test Development Team Lead | Department of Laboratory Medicine and Pathology | 480-301-8026 | Fax: 480-301-8372 | turner.mark@mayo.edu Mayo Clinic in Arizona | 13400 E. Shea Blvd. | Scottsdale, AZ 85259 | www.mayoclinic.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Thu Feb 24 12:44:34 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Feb 24 12:44:38 2011 Subject: [Histonet] Hematology Stainer Message-ID: <171633.22408.qm@web161720.mail.bf1.yahoo.com> Hi all. Does anyone use a small, desktop hematology stainer to perform Wright-Giemsa stains on bone marrow and blood smears??(We currently stain a dozen or fewer slides per day.)?If so, any information you could provide would be helpful. We are currently handstaining and have variations in staining. I'm looking to streamlining the staining? and stains used. Thank you, ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From laurie.colbert <@t> huntingtonhospital.com Thu Feb 24 12:47:59 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Feb 24 12:48:04 2011 Subject: [Histonet] Prostate needle core protocols In-Reply-To: <0CDA5E1E01301F4880A8A7A8BCBDA39C014ABE1D@mail.pcnm.com> References: <0CDA5E1E01301F4880A8A7A8BCBDA39C014ABE1D@mail.pcnm.com> Message-ID: <57BE698966D5C54EAE8612E8941D768301268FEF@EXCHANGE3.huntingtonhospital.com> We cut basically the same as you do - cut a ribbon and pick up two sections on one slide for H&E and two unstained sections on another slide; then cut in only about 8 microns and repeat two more times - for a total of 3 H&E slides and 3 unstained slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Thursday, February 24, 2011 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostate needle core protocols Hi all, my doc's want to know what protocol others use for cutting needle core biopsies of prostate. We cut a serial ribbon pick up one for H&E and the rest are kept as unstained slides, than trim in about 16-20 microns and take another serial section same as above and than repeat a third time. How do other labs cut their prostates biopsies? Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> sbcglobal.net Thu Feb 24 12:51:30 2011 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Thu Feb 24 12:51:33 2011 Subject: [Histonet] Thank you! In-Reply-To: References: Message-ID: <537442.8526.qm@web81208.mail.mud.yahoo.com> Here is a compilation of responses, I have removed names as I was not sure if it was?intentional to respond to offline: ? ? Cristi. Irun a GI Lab and we have request as you do. I just send it to the company they want the testing done by, and if this company is usually the same we will get there fed-x number and let them take care of the shipping and also they may or should have everything you need to ship to them. I would not send to a physicians office because they could lose your block. always send to one location and document that. any calls about insurance should be handled by the testing company send a Ins face sheet. Good luck and hope I helped you. ? Yes I do.? I have the outside physician fax me the order on his letterhead along with the patients signature and insurance. This way the outside facility can contact the ordering physician directly if any questions arise. Most of the outside facilities work with the ordering physician and the patients.? This has worked for me so far. ? We frequently get requests from physicians and send them to a reference lab that will 3rd party bill.? We have no financial responsibility and the doctor gets what he wants.? We do order the test in our system just so that we have a way in which to know what testing has been done and also track where the specimen was sent so that we can retrieve it.? I personally do not think it is a good idea to turn over the blocks to the requesting physician, this will create huge problems!? You are able to bill the patient for the "review of archived material." I can tell you from my experience that it is a lot of work and the hospital/lab does not benefit from it.? As for notifying the patient, we have never gotten a call from a patient regarding a bill.? The reference lab either accepts payment or works with the patient to handle the bill.? One thing you need to watch out for is Medicare patients regarding the 14 day rule.? Sometimes requests are made soon after the biopsy (within 14 days) and if the patient has Medicare, the reference lab will bill the hospital/lab. Hope this is helpful. ? ? Thank you again for all your guidance and have a great day all....only one more wake-up call :) ________________________________ From: "Rathborne, Toni" To: Cristi stephenson ; Histo Net Sent: Thu, February 24, 2011 10:00:50 AM Subject: RE: [Histonet] Thank you! Could you please share this information with the rest of us? The responses must not have gone back out to Histonet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cristi stephenson Sent: Thursday, February 24, 2011 12:58 PM To: Histo Net Subject: [Histonet] Thank you! Hello again, Wow!? What a wonderful world it would be if everyone were as quick and helpful as histoland.? Thank you all for the responses to my inquiry about outside physicians ordering testing, this information is very helpful and?I am excited to implement all of your ideas! Sincerely, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From jaylundgren <@t> gmail.com Thu Feb 24 14:22:56 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Feb 24 14:23:00 2011 Subject: [Histonet] Hematology Stainer In-Reply-To: <171633.22408.qm@web161720.mail.bf1.yahoo.com> References: <171633.22408.qm@web161720.mail.bf1.yahoo.com> Message-ID: Sheila, Some pathologists are fine substituting a Wright stain for a Giemsa stain, some are not. Although it is becoming more common to see the terminology "Wright/Giemsa", they are, of course, two different stains. Vendors sell Wright/Giemsa reagents, but that is a different matter. I have worked in labs where the smears were brought to the Histology lab, along with the clot and core, we picked out the best smear for an Iron stain, and then walked the rest down to Hematology to be put on the Wright stainer. Other pathologists specify a Giemsa stain. I would recommend asking your pathologist. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From relia1 <@t> earthlink.net Thu Feb 24 14:27:07 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Feb 24 14:27:10 2011 Subject: [Histonet] Specimen Processing Needed in New England. Can you help? Message-ID: Hi Histonetters! I am working with a leading pathology laboratory in New England that is in need of a specimen processing supervisor. This is a working supervisor position. My client needs someone with 2-3 years experience. ASCP or eligible. They offer excellent compensation, benefits, relocation assistance AND a sign on bonus. For more information contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. ***This position is also eligible for a $500.00 referral fee*** Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From foreightl <@t> gmail.com Thu Feb 24 14:27:04 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Thu Feb 24 14:27:13 2011 Subject: [Histonet] Prostate needle core protocols In-Reply-To: <57BE698966D5C54EAE8612E8941D768301268FEF@EXCHANGE3.huntingtonhospital.com> References: <0CDA5E1E01301F4880A8A7A8BCBDA39C014ABE1D@mail.pcnm.com> <57BE698966D5C54EAE8612E8941D768301268FEF@EXCHANGE3.huntingtonhospital.com> Message-ID: Our lab cuts 1 ribbon, takes a H&E and an Unstained parallel on the top of the slide, goes in about 16-20 microns, repeats the process 3 more times with sections on the top and bottom of 2 slides for H&E with a parallel unstained. On Thu, Feb 24, 2011 at 10:47 AM, Laurie Colbert wrote: > We cut basically the same as you do - cut a ribbon and pick up two > sections on one slide for H&E and two unstained sections on another > slide; then cut in only about 8 microns and repeat two more times - for > a total of 3 H&E slides and 3 unstained slides. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne > Clark > Sent: Thursday, February 24, 2011 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Prostate needle core protocols > > Hi all, my doc's want to know what protocol others use for cutting > needle core biopsies of prostate. ?We cut a serial ribbon pick up one > for H&E and the rest are kept as unstained slides, than trim in about > 16-20 microns and take another serial section same as above and than > repeat a third time. ?How do other labs cut their prostates biopsies? > > > > Joanne Clark, HT > > Histology Supervisor > > Pathology Consultants of New Mexico > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From cbrya <@t> lexclin.com Thu Feb 24 14:47:34 2011 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Feb 24 14:47:39 2011 Subject: [Histonet] procedural safeguards when accessioning identical sources Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BE623FA2@EXCHANGESB> Our lab has the policy of not accessioning back to back specimens of the same source. This helps us to ensure if there is a mix up the pathologist can tell when reading the case. For example if the specimen source is a skin and they have an endocervical they would know. We are soon to be getting a higher volume of prostates and will have to accession them together. What kind of procedural safeguards do you have in place when working with a high volume of identical sources? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From kkmarshall <@t> anthc.org Thu Feb 24 16:06:53 2011 From: kkmarshall <@t> anthc.org (Marshall, Kimberly K) Date: Thu Feb 24 16:07:09 2011 Subject: [Histonet] cdj Message-ID: Hello everyone We recently recieved a case possible positive for CJD. In researching this we have found that they now say Formalin is BAD. As long as I have been a Histo tech it seems the rules were Formalin then Formic acid. But seems there are some studies saying this is no longer enough. Is there anyone out there that has changed and if so What are you doing now??? Thanks in advance From sgoebel <@t> mirnarx.com Thu Feb 24 16:42:46 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Feb 24 16:42:51 2011 Subject: [Histonet] cdj In-Reply-To: References: Message-ID: Ahhh!! Everytime I hear those letters I have flash backs to my hospital days!! Stupid mad cow disease!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, February 24, 2011 4:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cdj Hello everyone We recently recieved a case possible positive for CJD. In researching this we have found that they now say Formalin is BAD. As long as I have been a Histo tech it seems the rules were Formalin then Formic acid. But seems there are some studies saying this is no longer enough. Is there anyone out there that has changed and if so What are you doing now??? Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Thu Feb 24 17:46:47 2011 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Feb 24 17:46:51 2011 Subject: [Histonet] cdj In-Reply-To: Message-ID: <2103241401.304662.1298591207519.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> The CDC website can tell you exactly what to do with a CJD case and it will surprise you. Pam Marcum UAMS ----- Original Message ----- From: "Kimberly K Marshall" To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 24, 2011 4:06:53 PM Subject: [Histonet] cdj Hello everyone ? ??We recently recieved a case possible positive for CJD. ?In researching this we have found that they now say Formalin is BAD. ?As long as I have been a Histo tech it seems the rules were Formalin then Formic acid. But seems there are some studies saying this is no longer enough. ?Is there anyone out there that has changed and if so What are you doing now??? ? ? ? Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Kimberly K Marshall" To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 24, 2011 4:06:53 PM Subject: [Histonet] cdj Hello everyone ? ??We recently recieved a case possible positive for CJD. ?In researching this we have found that they now say Formalin is BAD. ?As long as I have been a Histo tech it seems the rules were Formalin then Formic acid. But seems there are some studies saying this is no longer enough. ?Is there anyone out there that has changed and if so What are you doing now??? ? ? ? Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Thu Feb 24 18:13:41 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Feb 24 18:13:46 2011 Subject: [Histonet] Cassette Racks Message-ID: <0B8979A204680A42B93A52B486088CD9136B4D5DA9@CUAEXH1.GCU-MD.local> Does anyone still have any of those half racks that went in the VIP E300, if I recall correctly? They are stainless steel and do not have dividers. I have a friend in need of several and is willing to purchase them if necessary. Thanks as always! Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From william <@t> schuylerhouse.com Thu Feb 24 22:26:33 2011 From: william <@t> schuylerhouse.com (William Shipley) Date: Thu Feb 24 22:26:13 2011 Subject: [Histonet] Question from LIS vendor In-Reply-To: <171633.22408.qm@web161720.mail.bf1.yahoo.com> References: <171633.22408.qm@web161720.mail.bf1.yahoo.com> Message-ID: <41D77B378E7C421EA4608953333D99E8@Office> Hi, guys, I represent an LIS vendor in the clinical environment. We have a number of customers doing histology on our system, although it's primarily clinical in design. As we keep getting nudged in this direction, I've signed on to sort of 'soak up' the background. I've learned more about stains than I knew was possible already! One of our largest customers is in Malaysia and is doing a rather large amount of histology reports in addition to chemistry and hematology. During an inspection, an issue came up that we are trying to learn about. They took exception to the ability to alter a histology report once it had been 'finaled'. We've been told that it was unacceptable to even correct spelling errors and that any subsequent reporting had to be a separate report referring to the original. Is that practice in the US? I would have expected to have heard about it before. Can someone help me out? William Shipley Schuyler House From HornHV <@t> archildrens.org Fri Feb 25 07:16:23 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Feb 25 07:16:30 2011 Subject: [Histonet] procedural safeguards when accessioning identical sources In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BE623FA2@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BE623FA2@EXCHANGESB> Message-ID: <25A4DE08332B19499904459F00AAACB7194516A707@EVS1.archildrens.org> We use different colored cassettes. We have 6 different colors and rotate them with the cases. The color of the cassette is also dictated as part of the gross description. We use the same color of slides as the cassettes to carry this one step further. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, February 24, 2011 2:48 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] procedural safeguards when accessioning identical sources Our lab has the policy of not accessioning back to back specimens of the same source. This helps us to ensure if there is a mix up the pathologist can tell when reading the case. For example if the specimen source is a skin and they have an endocervical they would know. We are soon to be getting a higher volume of prostates and will have to accession them together. What kind of procedural safeguards do you have in place when working with a high volume of identical sources? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Stacy_McLaughlin <@t> cooley-dickinson.org Fri Feb 25 07:29:07 2011 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Feb 25 07:29:15 2011 Subject: [Histonet] leaking specimens Message-ID: Happy Friday Histonet! I'm wondering how folks out there deal with specimen containers that are leaking when received into the lab. Do you inform the physician's office, send it back, not inform anyone, etc? Thanks! Stacy McLaughlin, HT(ASCP) From trathborne <@t> somerset-healthcare.com Fri Feb 25 07:40:57 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Feb 25 07:41:26 2011 Subject: [Histonet] Question from LIS vendor In-Reply-To: <41D77B378E7C421EA4608953333D99E8@Office> Message-ID: Yes. After the final report is signed out, any changes, additions, or corrections have to be put through as an addendum. This becomes part of the patient's permanent record. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of William Shipley Sent: Thursday, February 24, 2011 11:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question from LIS vendor Hi, guys, I represent an LIS vendor in the clinical environment. We have a number of customers doing histology on our system, although it's primarily clinical in design. As we keep getting nudged in this direction, I've signed on to sort of 'soak up' the background. I've learned more about stains than I knew was possible already! One of our largest customers is in Malaysia and is doing a rather large amount of histology reports in addition to chemistry and hematology. During an inspection, an issue came up that we are trying to learn about. They took exception to the ability to alter a histology report once it had been 'finaled'. We've been told that it was unacceptable to even correct spelling errors and that any subsequent reporting had to be a separate report referring to the original. Is that practice in the US? I would have expected to have heard about it before. Can someone help me out? William Shipley Schuyler House _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From AHutton <@t> dh.org Fri Feb 25 08:05:07 2011 From: AHutton <@t> dh.org (Hutton, Allison) Date: Fri Feb 25 08:07:13 2011 Subject: [Histonet] procedure guidelines Message-ID: <38A56C4F4630D348A50B3720409270870E0FE2E9@dhmail.dhorg.org> We are in the process of revamping our procedures to be in the same format throughout the entire laboratory. I have been asked to find out if there is any format guidelines particularly for histology. We are using the CLSI standards but histo and cyto are finding that we don't quite "fit the mold" Thank you in advance, Allison From sfonner <@t> labpath.com Fri Feb 25 08:20:44 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Fri Feb 25 08:23:37 2011 Subject: [Histonet] IHC Equipment In-Reply-To: <000001cbd391$05b1ad00$11150700$@com> References: <000001cbd391$05b1ad00$11150700$@com> Message-ID: <000001cbd4f7$31001250$930036f0$@com> Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Fri Feb 25 08:30:56 2011 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Feb 25 08:31:07 2011 Subject: [Histonet] IHC Equipment In-Reply-To: <000001cbd4f7$31001250$930036f0$@com> References: <000001cbd391$05b1ad00$11150700$@com> <000001cbd4f7$31001250$930036f0$@com> Message-ID: <4D6784E0020000C80000F023@smtp1.gov.pe.ca> The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From NKonop <@t> chw.org Fri Feb 25 08:36:37 2011 From: NKonop <@t> chw.org (Konop, Nicole) Date: Fri Feb 25 08:36:42 2011 Subject: [Histonet] Diff quik and rapid H&E billing for touch preps Message-ID: Happy Friday! I am wondering if anyone can give me feedback in regards to touch prep billing when using diff quik and rapid H&E staining. Our facility uses both during frozen section. I am wondering if I can bill 88333 twice per part (one for rapid H&E, one for Diff quik) and then additional stains as 88334. Since they are two separate stains, even though they are touch preps, I am thinking I should be able to bill them individually as 88333 and if I do additional stains they would be billed as 88334. Any feedback is greatly appreciated. Thanks! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department From talulahgosh <@t> gmail.com Fri Feb 25 08:46:44 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Feb 25 08:46:48 2011 Subject: [Histonet] cdj In-Reply-To: <2103241401.304662.1298591207519.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <2103241401.304662.1298591207519.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Now I'm curious!! Please let us know what this mysterious processing is!! Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Thu, Feb 24, 2011 at 6:46 PM, Pamela Marcum wrote: > > > The CDC website can tell you exactly what to do with a CJD case and it will > surprise you. > > > > Pam Marcum > > UAMS > > > > > > ----- Original Message ----- > From: "Kimberly K Marshall" > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, February 24, 2011 4:06:53 PM > Subject: [Histonet] cdj > > Hello everyone > > We recently recieved a case possible positive for CJD. In researching > this we have found that they now say Formalin is BAD. As long as I have > been a Histo tech it seems the rules were Formalin then Formic acid. > But seems there are some studies saying this is no longer enough. Is > there anyone out there that has changed and if so What are you doing > now??? > > > Thanks in advance > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ----- Original Message ----- > From: "Kimberly K Marshall" > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, February 24, 2011 4:06:53 PM > Subject: [Histonet] cdj > > Hello everyone > > We recently recieved a case possible positive for CJD. In researching > this we have found that they now say Formalin is BAD. As long as I have > been a Histo tech it seems the rules were Formalin then Formic acid. > But seems there are some studies saying this is no longer enough. Is > there anyone out there that has changed and if so What are you doing > now??? > > > Thanks in advance > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mucram11 <@t> comcast.net Fri Feb 25 09:13:31 2011 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Feb 25 09:13:36 2011 Subject: [Histonet] cdj In-Reply-To: Message-ID: <1745858601.318356.1298646811134.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> The handling of CJD and other prion diseases is on the CDC website and that is the best place to understand the issues with equipment usage and contamination as well as procedures.? Many people have made comments however; the true issue is not just how to handle it but what you can not use again after you have used it for a CJD case or must quarantine and not use again for routine work. Pam Marcum UAMS? ----- Original Message ----- From: "Emily Sours" To: histonet@lists.utsouthwestern.edu Sent: Friday, February 25, 2011 8:46:44 AM Subject: Re: [Histonet] cdj Now I'm curious!! Please let us know what this mysterious processing is!! Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Thu, Feb 24, 2011 at 6:46 PM, Pamela Marcum wrote: > > > The CDC website can tell you exactly what to do with a CJD case and it will > surprise you. > > > > Pam Marcum > > UAMS > > > > > > ----- Original Message ----- > From: "Kimberly K Marshall" > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, February 24, 2011 4:06:53 PM > Subject: [Histonet] cdj > > Hello everyone > > ? We recently recieved a case possible positive for CJD. ?In researching > this we have found that they now say Formalin is BAD. ?As long as I have > been a Histo tech it seems the rules were Formalin then Formic acid. > But seems there are some studies saying this is no longer enough. ?Is > there anyone out there that has changed and if so What are you doing > now??? > > > Thanks in advance > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ----- Original Message ----- > From: "Kimberly K Marshall" > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, February 24, 2011 4:06:53 PM > Subject: [Histonet] cdj > > Hello everyone > > ? We recently recieved a case possible positive for CJD. ?In researching > this we have found that they now say Formalin is BAD. ?As long as I have > been a Histo tech it seems the rules were Formalin then Formic acid. > But seems there are some studies saying this is no longer enough. ?Is > there anyone out there that has changed and if so What are you doing > now??? > > > Thanks in advance > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Fri Feb 25 09:18:08 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Feb 25 09:18:12 2011 Subject: [Histonet] IHC Equipment In-Reply-To: <4D6784E0020000C80000F023@smtp1.gov.pe.ca> References: <000001cbd391$05b1ad00$11150700$@com> <000001cbd4f7$31001250$930036f0$@com> <4D6784E0020000C80000F023@smtp1.gov.pe.ca> Message-ID: In regards to the Ventana Ultra - if you are running PIN-4 or ISH simultaneously on this instrument, the kits/ab's/probes they require take up a lot of space on the reagent carousel which limits the number of antibodies you can put on and as runs can go some where around 6-hours you could find yourself limited in what you can run without careful planning. I'm a long time user and believer in Ventana, but BondMax and Intellipath do have my loyalties as well. It depends on the needs of your lab (volume/work flow, available lab space and antibody library size), the staffing you have available and their abilities with IHC/ISH. I've done this before and the only way to really know if an instrument will work in your lab is to demo them and connect with other users of the instrument. Vikki On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin wrote: > The Bond does do ISH. In fact it utilizes the same detection kit so you > have only to buy the probes, not the additional detection kit that could > (depending on your volume of ISH requests) expire. > Greg > > Greg Dobbin, R.T. > Chief Technologist, Anatomic Pathology > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > "I find that the harder I work, the > more luck I seem to have." > - Thomas Jefferson > > > >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> > Cindy, > > We used to use the Dako stainer, and we still have it as a back-up if > necessary, but we have recently (in the last year) bought a Ventana Ultra. > You can put 30 slides at a time on it, but you do not have to batch the > slides. It is a continuous feed machine, which means that as soon as a > slide is done, you can take it off and run something else. You do not have > to have all of the slides from a case together side by side. It is > bar-code > driven and will find the slides no matter where you put them. Each slide > drawer runs independently of the others. Ours has been wonderful. The > technical assistance is fantastic also. They will help you to initially > work up all of your antibodies. You can use theirs, or you can use third > party antibodies and place them in a prep-kit. You can also use RTU or > concentrates. It's really up to you. I would highly recommend it if you > have a large volume. We demo'd the Biocare Intellipath also. I liked the > machine, but it was really just a step up from the Dako. Leica has the > Bond > instruments, which a lot of people like, but for us, it didn't work because > we wanted to be able to do ISH. Also, the Leica limits you to drawers of > ten slides. So when you load a drawer, with 1 slide or 10, you can't use > it > again until that run is finished. Hope this helps. If you have any > questions, you can contact me. > Have a great day! > > Sheila > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia > Pyse > Sent: Wednesday, February 23, 2011 2:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC Equipment > > Hi Histonetters > > I currently use a Dako stainer for my IHC staining. It is a work horse with > very little problems. It is a older model that we may need to replace in > the > near future. What is everyone using out in histoland. I would be perfectly > willing to purchase another Dako but I want to explore all avenues before > making a decision. What are the pros and cons of the instruments any of you > are using. How often is the machine down? What is the capacity? We run the > Dako twice daily usually to the capacity of 48 slides. I would like to hear > only from actual user of the instrumentation, no vendors please. This is > only a fact finding e-mail. Thanks in advance for all your input. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory/Histology Supervisor > > X-Cell Laboratories > > 716-250-9235 > > e-mail cpyse@x-celllab.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or privileged > information intended for a specific individual or organization. If you have > received this communication in error, please notify the sender immediately. > If you are not the intended recipient, you are not authorized to use, > disclose, distribute, copy, print or rely on this email, and should promptly > delete this email from your entire computer system. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sfonner <@t> labpath.com Fri Feb 25 09:19:56 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Fri Feb 25 09:22:57 2011 Subject: [Histonet] IHC Equipment In-Reply-To: <4D6784E0020000C80000F023@smtp1.gov.pe.ca> References: <000001cbd391$05b1ad00$11150700$@com> <000001cbd4f7$31001250$930036f0$@com> <4D6784E0020000C80000F023@smtp1.gov.pe.ca> Message-ID: <001c01cbd4ff$761a86b0$624f9410$@com> Sorry if I confused anyone. It may have been FISH that we couldn't do on the Leica instrument. There was some reason that the docs chose to go with the Ventana. Anyway, I'm sure they are both fine instruments! Sheila From: Greg Dobbin [mailto:gvdobbin@ihis.org] Sent: Friday, February 25, 2011 9:31 AM To: Sheila Fonner; histonet@lists.utsouthwestern.edu; 'Cynthia Pyse' Subject: RE: [Histonet] IHC Equipment The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ------------------------- From a.byrnes <@t> accelpath.com Fri Feb 25 09:24:58 2011 From: a.byrnes <@t> accelpath.com (Andrew Byrnes) Date: Fri Feb 25 09:25:05 2011 Subject: [Histonet] Telepathology Message-ID: <14DA255A-0FBE-49BE-9046-43658A5F7171@accelpath.com> Dear Histonet, Thank you for your responses to my email this past week! If you have any additional thoughts about telepathology or digital pathology, please email me or call me directly. Have a nice weekend! Andrew Andrew Byrnes VP Sales and Marketing AccelPath, LLC M: 732-312-8008 www.AccelPath.com From cpyse <@t> x-celllab.com Fri Feb 25 09:24:09 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Feb 25 09:25:30 2011 Subject: [Histonet] procedural safeguards when accessioning identical sources In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BE623FA2@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF05BE623FA2@EXCHANGESB> Message-ID: <001401cbd500$0c9db710$25d92530$@com> Carol We mark all our prostates with dye for ease when embedding. For prostates that need to be accessioned in order we alternate the color of the dyes, hematoxylin, safranin, eosin. When grossing, the color of the dye used is in the dictation. Hope this helps. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, February 24, 2011 3:48 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] procedural safeguards when accessioning identical sources Our lab has the policy of not accessioning back to back specimens of the same source. This helps us to ensure if there is a mix up the pathologist can tell when reading the case. For example if the specimen source is a skin and they have an endocervical they would know. We are soon to be getting a higher volume of prostates and will have to accession them together. What kind of procedural safeguards do you have in place when working with a high volume of identical sources? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfonner <@t> labpath.com Fri Feb 25 09:25:06 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Fri Feb 25 09:28:04 2011 Subject: [Histonet] IHC Equipment In-Reply-To: References: <000001cbd391$05b1ad00$11150700$@com> <000001cbd4f7$31001250$930036f0$@com> <4D6784E0020000C80000F023@smtp1.gov.pe.ca> Message-ID: <002401cbd500$2ec2dd70$8c489850$@com> Vikki, You are absolutely right! We do all of our ISH as overnight runs to avoid the problem of space constraint with the detection kits, etc. I agree that all of the instruments have their good and bad points, and it really depends on the lab and what kind of specimens/volume you have. Didn't mean to offend anyone. I REALLY was just trying to help and give my opinion based on my own personal use. Sheila From: Victoria Baker [mailto:bakevictoria@gmail.com] Sent: Friday, February 25, 2011 10:18 AM To: Greg Dobbin Cc: Sheila Fonner; histonet@lists.utsouthwestern.edu; Cynthia Pyse Subject: Re: [Histonet] IHC Equipment In regards to the Ventana Ultra - if you are running PIN-4 or ISH simultaneously on this instrument, the kits/ab's/probes they require take up a lot of space on the reagent carousel which limits the number of antibodies you can put on and as runs can go some where around 6-hours you could find yourself limited in what you can run without careful planning. I'm a long time user and believer in Ventana, but BondMax and Intellipath do have my loyalties as well. It depends on the needs of your lab (volume/work flow, available lab space and antibody library size), the staffing you have available and their abilities with IHC/ISH. I've done this before and the only way to really know if an instrument will work in your lab is to demo them and connect with other users of the instrument. Vikki On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin wrote: The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Fri Feb 25 09:33:45 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Feb 25 09:33:54 2011 Subject: [Histonet] Deadline extended Message-ID: <9BF995BC0E47744E9673A41486E24EE22DBB52442F@MERCERMAIL.MercerU.local> Hi Everyone, Good news, the hotel extended the deadline for the GSH discount rate. As long as they have rooms they will honor the $99 @ night rate that includes continental breakfast and entrance to the park. There is no deadline for registering for the meeting, but for us to have name tags ready for you, please register beforehand. Information for the meeting. The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The complete program can be downloaded from our website at this link: www.histosearch.com/gsh> then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form on page 4. The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance. Come TRANSFORM yourselves. Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From cpyse <@t> x-celllab.com Fri Feb 25 09:38:20 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Feb 25 09:39:39 2011 Subject: [Histonet] Thanks Message-ID: <001501cbd502$07b27d60$17177820$@com> Happy Friday Histonetters I want to thank everyone for the information on IHC strainers. This is just what I needed. People who actually use the machines on a daily basis giving their opinions. What a great forum we have. Hope everyone has a great weekend. Currently we are receiving 6-10 inches of snow, hey, what do you except in Buffalo. Thanks again. Cindy Cindy Pyse, CLT, HT(ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com From talulahgosh <@t> gmail.com Fri Feb 25 09:40:20 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Feb 25 09:40:23 2011 Subject: [Histonet] Telepathology In-Reply-To: <14DA255A-0FBE-49BE-9046-43658A5F7171@accelpath.com> References: <14DA255A-0FBE-49BE-9046-43658A5F7171@accelpath.com> Message-ID: Okay, it's Friday so I have to write that my first thought was telepathology? Is that like telekinesis? Like processing slides and coverslipping with your mind? Would this extend to sectioning to? How about pouring your much needed cup of coffee, or even making the coffee? Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Fri, Feb 25, 2011 at 10:24 AM, Andrew Byrnes wrote: > Dear Histonet, > > Thank you for your responses to my email this past week! If you have any > additional thoughts about telepathology or digital pathology, please email > me or call me directly. > > Have a nice weekend! > > Andrew > > Andrew Byrnes > VP Sales and Marketing > AccelPath, LLC > M: 732-312-8008 > www.AccelPath.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria <@t> gmail.com Fri Feb 25 09:39:15 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Feb 25 09:44:54 2011 Subject: [Histonet] IHC Equipment In-Reply-To: <002401cbd500$2ec2dd70$8c489850$@com> References: <000001cbd391$05b1ad00$11150700$@com> <000001cbd4f7$31001250$930036f0$@com> <4D6784E0020000C80000F023@smtp1.gov.pe.ca> <002401cbd500$2ec2dd70$8c489850$@com> Message-ID: Sheila It is impossible to always get everything into an e-mail when you recieve a general info request. I thought what you said was great. Have a wonderful weekend. Vikki On Fri, Feb 25, 2011 at 10:25 AM, Sheila Fonner wrote: > Vikki, > > > > You are absolutely right! We do all of our ISH as overnight runs to avoid > the problem of space constraint with the detection kits, etc. I agree that > all of the instruments have their good and bad points, and it really depends > on the lab and what kind of specimens/volume you have. > > > > Didn?t mean to offend anyone. I REALLY was just trying to help and give my > opinion based on my own personal use. > > > > Sheila > > > > > > *From:* Victoria Baker [mailto:bakevictoria@gmail.com] > *Sent:* Friday, February 25, 2011 10:18 AM > *To:* Greg Dobbin > *Cc:* Sheila Fonner; histonet@lists.utsouthwestern.edu; Cynthia Pyse > *Subject:* Re: [Histonet] IHC Equipment > > > > In regards to the Ventana Ultra - if you are running PIN-4 or ISH > simultaneously on this instrument, the kits/ab's/probes they require take > up a lot of space on the reagent carousel which limits the number of > antibodies you can put on and as runs can go some where around 6-hours you > could find yourself limited in what you can run without careful planning. > > > > I'm a long time user and believer in Ventana, but BondMax and Intellipath > do have my loyalties as well. It depends on the needs of your lab > (volume/work flow, available lab space and antibody library size), the > staffing you have available and their abilities with IHC/ISH. I've done > this before and the only way to really know if an instrument will work in > your lab is to demo them and connect with other users of the instrument. > > > > Vikki > > > > > > > > > > > > > On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin wrote: > > The Bond does do ISH. In fact it utilizes the same detection kit so you > have only to buy the probes, not the additional detection kit that could > (depending on your volume of ISH requests) expire. > Greg > > Greg Dobbin, R.T. > Chief Technologist, Anatomic Pathology > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > "I find that the harder I work, the > more luck I seem to have." > - Thomas Jefferson > > > >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> > > Cindy, > > We used to use the Dako stainer, and we still have it as a back-up if > necessary, but we have recently (in the last year) bought a Ventana Ultra. > You can put 30 slides at a time on it, but you do not have to batch the > slides. It is a continuous feed machine, which means that as soon as a > slide is done, you can take it off and run something else. You do not have > to have all of the slides from a case together side by side. It is > bar-code > driven and will find the slides no matter where you put them. Each slide > drawer runs independently of the others. Ours has been wonderful. The > technical assistance is fantastic also. They will help you to initially > work up all of your antibodies. You can use theirs, or you can use third > party antibodies and place them in a prep-kit. You can also use RTU or > concentrates. It's really up to you. I would highly recommend it if you > have a large volume. We demo'd the Biocare Intellipath also. I liked the > machine, but it was really just a step up from the Dako. Leica has the > Bond > instruments, which a lot of people like, but for us, it didn't work because > we wanted to be able to do ISH. Also, the Leica limits you to drawers of > ten slides. So when you load a drawer, with 1 slide or 10, you can't use > it > again until that run is finished. Hope this helps. If you have any > questions, you can contact me. > Have a great day! > > Sheila > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia > Pyse > Sent: Wednesday, February 23, 2011 2:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC Equipment > > Hi Histonetters > > I currently use a Dako stainer for my IHC staining. It is a work horse with > very little problems. It is a older model that we may need to replace in > the > near future. What is everyone using out in histoland. I would be perfectly > willing to purchase another Dako but I want to explore all avenues before > making a decision. What are the pros and cons of the instruments any of you > are using. How often is the machine down? What is the capacity? We run the > Dako twice daily usually to the capacity of 48 slides. I would like to hear > only from actual user of the instrumentation, no vendors please. This is > only a fact finding e-mail. Thanks in advance for all your input. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory/Histology Supervisor > > X-Cell Laboratories > > 716-250-9235 > > e-mail cpyse@x-celllab.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or privileged > information intended for a specific individual or organization. If you have > received this communication in error, please notify the sender immediately. > If you are not the intended recipient, you are not authorized to use, > disclose, distribute, copy, print or rely on this email, and should promptly > delete this email from your entire computer system. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Allison_Scott <@t> hchd.tmc.edu Fri Feb 25 10:00:45 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Feb 25 10:00:50 2011 Subject: [Histonet] Checks and balances for specimen accessioning Message-ID: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From sgoebel <@t> mirnarx.com Fri Feb 25 10:12:25 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Feb 25 10:12:28 2011 Subject: [Histonet] Telepathology In-Reply-To: References: <14DA255A-0FBE-49BE-9046-43658A5F7171@accelpath.com> Message-ID: It's not nice to make fun of people's typing boo-boo's, but thanks for the Friday chuckle!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, February 25, 2011 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Telepathology Okay, it's Friday so I have to write that my first thought was telepathology? Is that like telekinesis? Like processing slides and coverslipping with your mind? Would this extend to sectioning to? How about pouring your much needed cup of coffee, or even making the coffee? Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Fri, Feb 25, 2011 at 10:24 AM, Andrew Byrnes wrote: > Dear Histonet, > > Thank you for your responses to my email this past week! If you have any > additional thoughts about telepathology or digital pathology, please email > me or call me directly. > > Have a nice weekend! > > Andrew > > Andrew Byrnes > VP Sales and Marketing > AccelPath, LLC > M: 732-312-8008 > www.AccelPath.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Fri Feb 25 10:13:19 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Feb 25 10:13:22 2011 Subject: [Histonet] Thanks In-Reply-To: <001501cbd502$07b27d60$17177820$@com> References: <001501cbd502$07b27d60$17177820$@com> Message-ID: It's sunshine and 75 here in Texas...nanny nanny...I do love my state's weather!! We had shorts on yesterday =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, February 25, 2011 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thanks Happy Friday Histonetters I want to thank everyone for the information on IHC strainers. This is just what I needed. People who actually use the machines on a daily basis giving their opinions. What a great forum we have. Hope everyone has a great weekend. Currently we are receiving 6-10 inches of snow, hey, what do you except in Buffalo. Thanks again. Cindy Cindy Pyse, CLT, HT(ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Fri Feb 25 10:22:34 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Feb 25 10:22:40 2011 Subject: [Histonet] Telepathology In-Reply-To: References: <14DA255A-0FBE-49BE-9046-43658A5F7171@accelpath.com> Message-ID: I believe that Telepathology should be the name of what one of our pathologists wanted us to do. "Cut to the small focus of tumor and stop". On Fri, Feb 25, 2011 at 8:12 AM, wrote: > It's not nice to make fun of people's typing boo-boo's, but thanks for the Friday chuckle!! > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas ?78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Friday, February 25, 2011 9:40 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Telepathology > > Okay, it's Friday so I have to write that my first thought was > telepathology? Is that like telekinesis? Like processing slides and > coverslipping with your mind? Would this extend to sectioning to? How about > pouring your much needed cup of coffee, or even making the coffee? > > Emily > > It has become almost a cliche to remark that nobody boasts of ignorance of > literature, but it is socially acceptable to boast ignorance of science and > proudly claim incompetence in mathematics. > -Richard Dawkins > > > > On Fri, Feb 25, 2011 at 10:24 AM, Andrew Byrnes wrote: > >> Dear Histonet, >> >> Thank you for your responses to my email this past week! ?If you have any >> additional thoughts about telepathology or digital pathology, please email >> me or call me directly. >> >> Have a nice weekend! >> >> Andrew >> >> Andrew Byrnes >> VP Sales and Marketing >> AccelPath, LLC >> M: 732-312-8008 >> www.AccelPath.com >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From mike <@t> pathview.com Fri Feb 25 10:22:50 2011 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Feb 25 10:23:11 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> Message-ID: <00cc01cbd508$41318490$c3948db0$@pathview.com> To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Fri Feb 25 10:25:42 2011 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Feb 25 10:25:59 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <00cc01cbd508$41318490$c3948db0$@pathview.com> References: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> <00cc01cbd508$41318490$c3948db0$@pathview.com> Message-ID: <4D6783A6.5D38.00EF.0@swmail.sw.org> You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Michael Mihalik" 2/25/2011 10:22 AM >>> To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From marktarango <@t> gmail.com Fri Feb 25 10:40:48 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Feb 25 10:40:54 2011 Subject: [Histonet] Thanks In-Reply-To: References: <001501cbd502$07b27d60$17177820$@com> Message-ID: So cruel! On Fri, Feb 25, 2011 at 8:13 AM, wrote: > It's sunshine and 75 here in Texas...nanny nanny...I do love my state's > weather!! We had shorts on yesterday =) > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia > Pyse > Sent: Friday, February 25, 2011 9:38 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Thanks > > Happy Friday Histonetters > > I want to thank everyone for the information on IHC strainers. This is > just > what I needed. People who actually use the machines on a daily basis > giving > their opinions. What a great forum we have. Hope everyone has a great > weekend. Currently we are receiving 6-10 inches of snow, hey, what do > you > except in Buffalo. Thanks again. > > Cindy > > > > Cindy Pyse, CLT, HT(ASCP) > > Laboratory/Histology Supervisor > > X-Cell Laboratories > > 716-250-9235 > > e-mail cpyse@x-celllab.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From contact <@t> excaliburpathology.com Fri Feb 25 10:53:34 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Feb 25 10:53:37 2011 Subject: [Histonet] Thanks In-Reply-To: References: <001501cbd502$07b27d60$17177820$@com> Message-ID: <798658.91154.qm@web1114.biz.mail.sk1.yahoo.com> Texas Rocks!!!!!?2 years and 2 months until?I'm home. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "sgoebel@mirnarx.com" To: cpyse@x-celllab.com; histonet@lists.utsouthwestern.edu Sent: Fri, February 25, 2011 10:13:19 AM Subject: RE: [Histonet] Thanks It's sunshine and 75 here in Texas...nanny nanny...I do love my state's weather!!? We had shorts on yesterday =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, February 25, 2011 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thanks Happy Friday Histonetters I want to thank everyone for the information on IHC strainers. This is just what I needed. People who actually use the machines on a daily basis giving their opinions. What a great forum we have. Hope everyone has a great weekend. Currently we are receiving 6-10 inches of snow, hey, what do you except in Buffalo. Thanks again. Cindy Cindy Pyse, CLT, HT(ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Fri Feb 25 11:11:53 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Feb 25 11:12:05 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <4D6783A6.5D38.00EF.0@swmail.sw.org> References: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> <00cc01cbd508$41318490$c3948db0$@pathview.com> <4D6783A6.5D38.00EF.0@swmail.sw.org> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73E1@EXCHMBC2.ad.ah.local> Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets "hard-wired" after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Michael Mihalik" 2/25/2011 10:22 AM >>> To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From NMargaryan <@t> childrensmemorial.org Fri Feb 25 11:18:34 2011 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Feb 25 11:19:21 2011 Subject: [Histonet] kits for LCM & archival FFPE tissue Message-ID: Hi all, I am about to perform PCR on archival FFPE tissue after LCM. My LCM system is from Arcturus and my tissue is on membraned glass slides. My questions are: 1. What Kit to use to get the highest yields and quality RNA? 2. Can anyone who has been doing it for the past 2-3 years suggest me a good amplification and purification kit for FFPE tissue? 3. Can you please send me the serial numbers of the kits? Thanks in advance, Naira From mike <@t> pathview.com Fri Feb 25 11:47:16 2011 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Feb 25 11:47:46 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73E1@EXCHMBC2.ad.ah.local> References: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> <00cc01cbd508$41318490$c3948db0$@pathview.com> <4D6783A6.5D38.00EF.0@swmail.sw.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73E1@EXCHMBC2.ad.ah.local> Message-ID: <001801cbd514$1200dde0$360299a0$@pathview.com> ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets "hard-wired" after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Michael Mihalik" 2/25/2011 10:22 AM >>> To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From SFinley <@t> providencehealth.bc.ca Fri Feb 25 12:08:24 2011 From: SFinley <@t> providencehealth.bc.ca (Finley, Sue [PH]) Date: Fri Feb 25 12:08:29 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <001801cbd514$1200dde0$360299a0$@pathview.com> References: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> <00cc01cbd508$41318490$c3948db0$@pathview.com> <4D6783A6.5D38.00EF.0@swmail.sw.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73E1@EXCHMBC2.ad.ah.local> <001801cbd514$1200dde0$360299a0$@pathview.com> Message-ID: <7D627F9CD9AE514087E447A9716D4D359A5D5147@vchexmbp14.vch.ca> Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle "external incidents" of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets "hard-wired" after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Michael Mihalik" 2/25/2011 10:22 AM >>> To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Fri Feb 25 12:11:58 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Feb 25 12:12:09 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <7D627F9CD9AE514087E447A9716D4D359A5D5147@vchexmbp14.vch.ca> References: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> <00cc01cbd508$41318490$c3948db0$@pathview.com> <4D6783A6.5D38.00EF.0@swmail.sw.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73E1@EXCHMBC2.ad.ah.local> <001801cbd514$1200dde0$360299a0$@pathview.com> <7D627F9CD9AE514087E447A9716D4D359A5D5147@vchexmbp14.vch.ca> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73E4@EXCHMBC2.ad.ah.local> Same here Sue, thanks for the reply. Jan -----Original Message----- From: Finley, Sue [PH] [mailto:SFinley@providencehealth.bc.ca] Sent: Friday, February 25, 2011 12:08 PM To: 'Michael Mihalik'; Mahoney,Janice A; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle "external incidents" of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets "hard-wired" after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Michael Mihalik" 2/25/2011 10:22 AM >>> To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From mike <@t> pathview.com Fri Feb 25 12:48:49 2011 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Feb 25 12:49:21 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <7D627F9CD9AE514087E447A9716D4D359A5D5147@vchexmbp14.vch.ca> References: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> <00cc01cbd508$41318490$c3948db0$@pathview.com> <4D6783A6.5D38.00EF.0@swmail.sw.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73E1@EXCHMBC2.ad.ah.local> <001801cbd514$1200dde0$360299a0$@pathview.com> <7D627F9CD9AE514087E447A9716D4D359A5D5147@vchexmbp14.vch.ca> Message-ID: <004c01cbd51c$abbc8350$033589f0$@pathview.com> One quick clarification: If the OR or whomever collects the specimen would put a barcoded label on the specimen container at collection time, all of this would be avoided. That just seems to be a difficult thing for those departments to do. I know that it's not a technical issue. It's something more than that. ..but it can be done and in what appears to be a small percentage of facilities, is indeed done. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Finley, Sue [PH] [mailto:SFinley@providencehealth.bc.ca] Sent: Friday, February 25, 2011 10:08 AM To: 'Michael Mihalik'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle "external incidents" of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets "hard-wired" after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Michael Mihalik" 2/25/2011 10:22 AM >>> To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Feb 25 13:03:08 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Feb 25 13:03:15 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <004c01cbd51c$abbc8350$033589f0$@pathview.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B4E@is-e2k3.grhs.net> But you have to hope the whom ever is putting the barcode label on uses the correct patient barcode. That has been the problem that I have seen. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, February 25, 2011 12:49 PM To: 'Finley, Sue [PH]'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning One quick clarification: If the OR or whomever collects the specimen would put a barcoded label on the specimen container at collection time, all of this would be avoided. That just seems to be a difficult thing for those departments to do. I know that it's not a technical issue. It's something more than that. ..but it can be done and in what appears to be a small percentage of facilities, is indeed done. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Finley, Sue [PH] [mailto:SFinley@providencehealth.bc.ca] Sent: Friday, February 25, 2011 10:08 AM To: 'Michael Mihalik'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle "external incidents" of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets "hard-wired" after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Michael Mihalik" 2/25/2011 10:22 AM >>> To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Feb 25 13:14:19 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Feb 25 13:14:23 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <004c01cbd51c$abbc8350$033589f0$@pathview.com> References: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> <00cc01cbd508$41318490$c3948db0$@pathview.com> <4D6783A6.5D38.00EF.0@swmail.sw.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73E1@EXCHMBC2.ad.ah.local> <001801cbd514$1200dde0$360299a0$@pathview.com> <7D627F9CD9AE514087E447A9716D4D359A5D5147@vchexmbp14.vch.ca> <004c01cbd51c$abbc8350$033589f0$@pathview.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EB7EE024F@IBMB7Exchange.digestivespecialists.com> That's my goal in the next year! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, February 25, 2011 1:49 PM To: 'Finley, Sue [PH]'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning One quick clarification: If the OR or whomever collects the specimen would put a barcoded label on the specimen container at collection time, all of this would be avoided. That just seems to be a difficult thing for those departments to do. I know that it's not a technical issue. It's something more than that. ..but it can be done and in what appears to be a small percentage of facilities, is indeed done. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Finley, Sue [PH] [mailto:SFinley@providencehealth.bc.ca] Sent: Friday, February 25, 2011 10:08 AM To: 'Michael Mihalik'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle "external incidents" of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets "hard-wired" after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> "Michael Mihalik" 2/25/2011 10:22 AM >>> To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. 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Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Fri Feb 25 13:15:06 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Feb 25 13:15:11 2011 Subject: [Histonet] cdj In-Reply-To: References: <2103241401.304662.1298591207519.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <25A4DE08332B19499904459F00AAACB7194516A70D@EVS1.archildrens.org> This is what I found at the CDC website: "Table 9. Tissue Preparation for Human CJD and Related Diseases 1. Histology technicians wear gloves, apron, laboratory coat, and face protection. 2. Adequate fixation of small tissue samples (e.g., biopsies) from a patient with suspected prion disease can be followed by post-fixation in 96% absolute formic acid for 30 minutes, followed by 45 hours in fresh 10% formalin. 3. Liquid waste is collected in a 4L waste bottle initially containing 600 ml 6N NaOH. 4. Gloves, embedding molds, and all handling materials are disposed s regulated medical waste. 5. Tissue cassettes are processed manually to prevent contamination of tissue processors. 6. Tissues are embedded in a disposable embedding mold. If used, forceps are decontaminated as in Table 10. 7. In preparing sections, gloves are worn, section waste is collected and disposed in a regulated medical waste receptacle. The knife stage is wiped with 2N NaOH, and the knife used is discarded immediately in a ?regulated medical waste sharps? receptacle. Slides are labeled with ?CJD Precautions.? The sectioned block is sealed with paraffin. 8. Routine staining: a. slides are processed by hand; b. reagents are prepared in 100 ml disposable specimen cups; c. after placing the cover slip on, slides are decontaminated by soaking them for 1 hour in 2N NaOH; d. slides are labeled as ?Infectious-CJD.? 9. Other suggestions: a. disposable specimen cups or slide mailers may be used for reagents; b. slides for immunocytochemistry may be processed in disposable Petri dishes; c. equipment is decontaminated as described above or disposed as regulated medical waste. Handling and processing of tissues from patients with suspected prion disease The special characteristics of work with prions require particular attention to the facilities, equipment, policies, and procedures involved.10 The related considerations outlined in Table 9 should be incorporated into the laboratory?s risk management for this work. 288 Biosafety in Microbiological and Biomedical Laboratories Table 10. Prion Inactivation Methods for Reusable Instruments and Surfaces 1. Immerse in 1 N NaOH, heat in a gravity displacement autoclave at 121?C for 30 minutes. Clean and sterilize by conventional means. 2. Immerse in 1 N NaOH or sodium hypochlorite (20,000 ppm) for 1 hours. Transfer into water and autoclave (gravity displacement) at 121?C for 1 hour. Clean and sterilize by conventional means. 3. Immerse in 1N NaOH or sodium hypochlorite (20,000) for 1 hour. Rinse instruments with water, transfer to open pan and autoclave at 121?C (gravity displacement) or 134?C (porous load) for 1 hour. Clean and sterilize by conventional means. 4. Surfaces or heat-sensitive instruments can be treated with 2N NaOH or sodium hypochlorite (20,000 ppm) for 1 hour. Ensure surfaces remain wet for entire period, then rinse well with water. Before chemical treatment, it is strongly recommended that gross contamination of surfaces be reduced because the presence of excess organic material will reduce the strength of either NaOH or sodium hypochlorite solutions. 5. Environ LpH (EPA Reg. No. 1043-118) may be used on washable, hard, non-porous surfaces (such as floors, tables, equipment, and counters), items (such as non-disposable instruments, sharps, and sharp containers), and/or laboratory waste solutions (such as formalin or other liquids). This product is currently being used under FIFRA Section 18 exemptions in a number of states. Users should consult with the state environmental protection office prior to use. (Adapted from www.cdc.gov 11,12) Working Solutions 1 N NaOH equals 40 grams of NaOH per liter of water. Solution should be prepared daily. A stock solution of 10 N NaOH can be prepared and fresh 1:10 dilutions (1 part 10 N NaOH plus 9 parts water) used daily. 20,000 ppm sodium hypochlorite equals a 2% solution. Most commercial household bleach contains 5.25% sodium hypochlorite, therefore, make a 1:2.5 dilution (1 part 5.25% bleach plus 1.5 parts water) to produce a 20,000 ppm solution. This ratio can also be stated as two parts 5.25% bleach to three parts water. Working solutions should be prepared daily. CAUTION: Above solutions are corrosive and require suitable personal protective equipment and proper secondary containment. These strong corrosive solutions require careful disposal in accordance with local regulations. Precautions in using NaOH or sodium hypochlorite solutions in autoclaves: NaOH spills or gas may damage the autoclave if proper containers are not used. The use of containers with a rim and lid designed for condensation to collect and drip back into the pan is recommended. Persons who use this procedure should be cautious in handling hot NaOH solution (post-autoclave) and in avoiding potential exposure to gaseous NaOH; exercise caution during all sterilization steps; and allow the autoclave, instruments, and solutions to cool down before removal. Immersion in sodium hypochlorite bleach can cause severe damage to some instruments." Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, February 25, 2011 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cdj Now I'm curious!! Please let us know what this mysterious processing is!! Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Thu, Feb 24, 2011 at 6:46 PM, Pamela Marcum wrote: > > > The CDC website can tell you exactly what to do with a CJD case and it will > surprise you. > > > > Pam Marcum > > UAMS > > > > > > ----- Original Message ----- > From: "Kimberly K Marshall" > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, February 24, 2011 4:06:53 PM > Subject: [Histonet] cdj > > Hello everyone > > We recently recieved a case possible positive for CJD. In researching > this we have found that they now say Formalin is BAD. As long as I have > been a Histo tech it seems the rules were Formalin then Formic acid. > But seems there are some studies saying this is no longer enough. Is > there anyone out there that has changed and if so What are you doing > now??? > > > Thanks in advance > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ----- Original Message ----- > From: "Kimberly K Marshall" > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, February 24, 2011 4:06:53 PM > Subject: [Histonet] cdj > > Hello everyone > > We recently recieved a case possible positive for CJD. In researching > this we have found that they now say Formalin is BAD. As long as I have > been a Histo tech it seems the rules were Formalin then Formic acid. > But seems there are some studies saying this is no longer enough. Is > there anyone out there that has changed and if so What are you doing > now??? > > > Thanks in advance > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From bakevictoria <@t> gmail.com Fri Feb 25 13:16:04 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Feb 25 13:21:10 2011 Subject: [Histonet] Checks and balances for specimen accessioning In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFD71@LBEXCH01.hchd.local> Message-ID: Do you recall the source of pick up for the specimen? If it is picked up by Histology either in the OR or a lab site the pick up person should be checking the name on requisition, container and label in the book or ledger prior to initialing and dating. Also the person bringing the specimen to these sites has to initial and date when they put the pt. label in the ledger. I would go back to the source - as a starting point. Once the specimen is received into the lab, the person accessioning is checking the container against the requisition. Once a patient is registered either in the hospital or at the site they are assigned a medical record number or another unique identifier. When you accession this patient by one of these identifiers the descrepancy should (I stress should) show up and be caught. Another check is to see if the specimen on the container matches what is on the requisition. Something which should be done from pick up point. It varies by site how the specimen is dictated in regards to specimen ID. Some read from the bottle while looking at the requisition and dictate from that, but I've found it isn't always the rule. The original label (source label) has to be clearly visible and not covered with another label at any time in the process. If you are using a barcode label in the Histology lab the barcode needs to be placed way from the source label. The LEAN process does indeed standardize a lot of the work process, but it is also based on the lab volume, work flow which is individual to each lab. Also the use of computer systems and how fully they are utilized within each lab. I've worked in several labs that are LEAN and they still had issues with specimen handling/verification for different reasons. In your situation you would have to follow the paper trail at this point, but it would be a very strong reason to work towards getting a unified system in place that tracks specimens from the source to final sign out. I know that there are companies out there that do this one is PathCentral they provide software and support. Have a great weekend. Vikki On Fri, Feb 25, 2011 at 11:00 AM, Scott, Allison D < Allison_Scott@hchd.tmc.edu> wrote: > Hello to all in histoland. What types of checks and balances do you > have in place for specimen accessioning. We had a incidcent where I was > accessioning a case and I did not catch that the name on the container > was different from the name on the requisition. The resident grossing > did not catch it either. They usually peel back the copath label and > look at the name on the label that came from the procedure area. In my > case the resident did not do this. It was not until the pathologist saw > a discrepancy in the age on the requisition and what was written in the > pertinent history, that it was determined that it had been mislabeled > from the beginning. I did a incident report and the area was cited. > Besides making sure that who ever is accesioning cases checks that the > names match, what else can be done? Any help in this will be greatly > appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kkmarshall <@t> anthc.org Fri Feb 25 14:34:31 2011 From: kkmarshall <@t> anthc.org (Marshall, Kimberly K) Date: Fri Feb 25 14:35:19 2011 Subject: [Histonet] Poor nuclear artifact Message-ID: Happy Friday Histo people!!! I need some opinions on a processing issue I cant seem to fix. I run a short processing run each night for my small biopsies. With two processors, I rotate so the machine with the newest reagents is the one we run as the biopsy machine. For several weeks now my Pathologist have had issues with one or two blocks a day or one day it was one piece of tissue of many in a GI biopsy block,(only one piece of like 5 were affected the rest were fine) that they are saying has "poor nuclear artifact" . Most days it is only one block and has happend only once with tissue from the long run. This happens no matter a weekend or over night, fresh reagents or not. I have biopsies come from all over Alaska and in talking with them as well as checking the containers they are submitting in 10% NBF, The stainer is rotated daily so its not a Xylene not clearing the slide problem. I know sections can be thicker in a ribbon but the Pathologist dont seem to think that is the problem either. I have run out of things it could be and could really use some advise. Thanks and have a great weekend From rjbuesa <@t> yahoo.com Fri Feb 25 15:13:56 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 25 15:14:02 2011 Subject: [Histonet] Poor nuclear artifact In-Reply-To: Message-ID: <625759.16311.qm@web65707.mail.ac4.yahoo.com> My problem in understanding your difficulty is the definition: what is "poor nuclear artifact"? No detail? Weak staining? Strong staining? "Empty" nuclus?? The cause of the problem will vary depending on its definition. Unless you are more specific I think it will be?very difficult in trying to help you. Please be more specific Ren? J. --- On Fri, 2/25/11, Marshall, Kimberly K wrote: From: Marshall, Kimberly K Subject: [Histonet] Poor nuclear artifact To: histonet@lists.utsouthwestern.edu Date: Friday, February 25, 2011, 3:34 PM Happy Friday Histo people!!! ? I need some opinions on a processing issue I cant seem to fix. ? I run a short processing run each night for my small biopsies. With two processors,? I rotate so the machine with the newest reagents is the one we run as the biopsy machine.? For several weeks now my Pathologist have had issues with one or two blocks a day or one day it was one piece of tissue of many in a GI biopsy block,(only one piece of like 5 were affected the rest were fine) that they are saying has "poor nuclear artifact" .???Most days it is only one block and has happend only once with tissue from the long run.? This happens no matter a weekend or over night, fresh reagents or not.? I have biopsies come from all over Alaska and in talking with them as well as checking the containers they are submitting in 10% NBF,? The stainer is rotated daily so its not a Xylene not clearing the slide problem.? I know sections can be thicker in a ribbon but the Pathologist dont seem to think that is the problem either. I have run out of things it could be and could really use some advise. Thanks and have a great weekend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyer3322 <@t> aol.com Sat Feb 26 06:38:54 2011 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sat Feb 26 06:39:00 2011 Subject: [Histonet] TEXAS SOCIETY FOR HISTOTECHNOLOGY 2011 SYMPOSIUM/CONVENTION Message-ID: <8CDA3C943106D3D-1DC8-9946@Webmail-m107.sysops.aol.com> All, It's not too late to sign up for the TSH meeting in April! The TSH meeting will be held March 31-April 3, 2011. The meeting will start with a Vendor/Attendee Golf Outing. There will be Food, Fun and Prizes! Contact RebeccaRuser@mhd.com for registration or information. The meeting will begin Friday April 1, 2011 with 2 Workshops and the opening of the Vendor Exhibits. We have to date 30 Vendors represented. Contact Sandra Christiansen at sandra.christiansen@christushealth.org for vendor information or to exhibit. It is not to late to sign up for a variety of interesting symposiums and workshops. Pre-registration deadline is March 25, 2011. There are still plenty of rooms at the Marriott Dallas/Plano at the Legacy Twon Center, 7120 Dallas Parkway, Plano Texas. Room rates are $109.00/night. Contact 972-473-6444 to book a room. Hotel deadline is March 10, 2011. If you would like a copy of the TSH program electronically please contact Kathy Dwyer via this e-mail or kdwyer3322@aol.com We hope to see you in TEXAS! Regards, TSH Convention Committee From histotech.jp <@t> charter.net Sat Feb 26 11:08:35 2011 From: histotech.jp <@t> charter.net (Jim Pepoon) Date: Sat Feb 26 11:08:48 2011 Subject: [Histonet] LABLION In-Reply-To: Message-ID: <13F71A9BB3894D4781E3FF91BCCB166C@JimPC> I've recently seen a demonstration of the most amazing pathology management system to exist in the entire world (and I know, because I've been all over the world looking at this stuff). I'm seriously considering acquisition, as are a number of AP labs in the Northeast. If you go to www.lablion.com you will see the future of patient safe, regulatory compliance, paperless, lean and management workflow, infinitely integrate-able, can completely stand alone or interface with any LIS, can interface with anyone's equipment, Anatomic Pathology Management system ....Oh, yeah, that happens to also barcode and track everything. Lablion is completely open and flexible (not even LIS dependent...if you can wrap your mind around that), and doesn't swear allegiance to any equipment or LIS company's wares. Lablion is custom installed to the needs and wants of your specific lab - no two installs are the same. They don't have a 'lean' marketing consultant come and help you to figure out how you should change your lab to fit their product. As I'm sure I'm not alone on this: I can say that we've all grown frustrated with industry slapping a 2d barcode on something and now claiming 'we have a tracking system'....or 'we have a system coming...in like two years'. Anyone can print a barcode - it's what you do with the information from the scan events that count. Equally frustrating are equipment companies who's motivation is really to sell expensive instruments and reagents at ++ $ per test - NOT patient safety...or LIS companies beta testing bar-coding modules and force you purchase the LIS upgrade for hundreds of thousands of dollars so that you can get their tracking system for free, etc. etc. I encourage you to not my word for this. Go ahead and compare Lablion side-by-side next to anyone else's system and evaluate for yourself. You'll be glad you spent the time to do this. From Dana.Spencer <@t> PCMH.COM Mon Feb 28 08:41:02 2011 From: Dana.Spencer <@t> PCMH.COM (Dana Spencer) Date: Mon Feb 28 08:41:42 2011 Subject: [Histonet] Histology Workflow Solutions Message-ID: <4D6B6DAE.536C.000A.0@PCMH.COM> I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! Dana ------------------------------------------------------------------------------ The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. ============================================================================== From christiegowan <@t> msn.com Mon Feb 28 09:04:45 2011 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Mon Feb 28 09:04:52 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: <4D6B6DAE.536C.000A.0@PCMH.COM> References: <4D6B6DAE.536C.000A.0@PCMH.COM> Message-ID: Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ------------------------------------------------------------------------------ > The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ============================================================================== > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Mon Feb 28 09:55:26 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Feb 28 09:55:37 2011 Subject: [Histonet] parts for CV5030 glass coverslipper Message-ID: <4D6B7F1E.2B7F.00C9.1@geisinger.edu> Does anyone know if you can buy these from someone else besides Leica? My Fisher rep never got back to me. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From MSHERWOOD <@t> PARTNERS.ORG Mon Feb 28 10:04:29 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Feb 28 10:04:35 2011 Subject: [Histonet] parts for CV5030 glass coverslipper In-Reply-To: <4D6B7F1E.2B7F.00C9.1@geisinger.edu> References: <4D6B7F1E.2B7F.00C9.1@geisinger.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB54CB@PHSXMB30.partners.org> We have a refurbished CV5030 coverslipper and we ordered directly from Leica. If someone knows of anyone else who sells the parts/supplies, I would also be interested. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Monday, February 28, 2011 10:55 AM To: histonet Subject: [Histonet] parts for CV5030 glass coverslipper Does anyone know if you can buy these from someone else besides Leica? My Fisher rep never got back to me. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From bakevictoria <@t> gmail.com Mon Feb 28 11:26:07 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Feb 28 11:26:11 2011 Subject: [Histonet] parts for CV5030 glass coverslipper In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB54CB@PHSXMB30.partners.org> References: <4D6B7F1E.2B7F.00C9.1@geisinger.edu> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB54CB@PHSXMB30.partners.org> Message-ID: Have you tried contacting Belair Instruments? I believe they are located in Fanwood NJ. Angie if you get a chance contact me off server :-). On Feb 28, 2011 11:04 AM, "Sherwood, Margaret" wrote: > We have a refurbished CV5030 coverslipper and we ordered directly from Leica. > If someone knows of anyone else who sells the parts/supplies, I would also be > interested. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting > Sent: Monday, February 28, 2011 10:55 AM > To: histonet > Subject: [Histonet] parts for CV5030 glass coverslipper > > Does anyone know if you can buy these from someone else besides Leica? My Fisher > rep never got back to me. > > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > IMPORTANT WARNING: The information in this message (and the documents attached > to it, if any) is confidential and may be legally privileged. It is intended > solely for the addressee. Access to this message by anyone else is unauthorized. > If you are not the intended recipient, any disclosure, copying, distribution or > any action taken, or omitted to be taken, in reliance on it is prohibited and > may be unlawful. If you have received this message in error, please delete all > electronic copies of this message (and the documents attached to it, if any), > destroy any hard copies you may have created and notify me immediately by > replying to this email. Thank you. > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Mon Feb 28 11:35:01 2011 From: mike <@t> pathview.com (Michael Mihalik) Date: Mon Feb 28 11:35:13 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: References: <4D6B6DAE.536C.000A.0@PCMH.COM> Message-ID: <010a01cbd76d$d57dce30$80796a90$@pathview.com> As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Mon Feb 28 11:39:56 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Feb 28 11:40:12 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: <010a01cbd76d$d57dce30$80796a90$@pathview.com> References: <4D6B6DAE.536C.000A.0@PCMH.COM> <010a01cbd76d$d57dce30$80796a90$@pathview.com> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D6976@EXCHANGECLUSTER.yumaregional.local> Well put Michael!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, February 28, 2011 10:35 AM To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From JEllin <@t> yumaregional.org Mon Feb 28 11:41:06 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Feb 28 11:41:15 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8035D6975@EXCHANGECLUSTER.yumaregional.local> References: <4D6B6DAE.536C.000A.0@PCMH.COM> <29BE166A2CF48D459853F8EC57CD37E8035D6975@EXCHANGECLUSTER.yumaregional.local> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D6977@EXCHANGECLUSTER.yumaregional.local> Hello Dana, Well I see you have the golden question, which one do you choose and which one you stick with. My perception is that both add value and both are going to give you what you are looking for in the short term. But in the long run as we dive deeper into the functionality of both it becomes the cat and mouse game. Who is going to give what information and who is going to store the information? I think you need to weigh out the Pros and Cons for your institution and take into consideration the normal items of value, cost, and impact. But the items not being discussed by either side is informatics (free flow of data no matter what the situation, think about EHR here), data mining ( full view of the process to include all steps, other than scanned sites), flexibility (meaning not having to be a single vendor centric or being able to pull data from different instrumentation.) What has happened is due to recent regulatory changes, everyone is racing to find a solution to meet the need of compliance as well as increase efficiency due to cut backs. But remember this is a culture change and begins with a lot of bumps and bruises. I would also offer up other stand alone systems that you need to look at. I myself am a PowerPath user and I have had this debate for a long time. But I in my current situation, I am seeing that EHR's are playing a role in this as well. Especially if you are looking at going with the EHR system. Feel free to contact me if you have questions. Jesus Ellin Yuma Regional Medical Center 928-336-1743 ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Janice.Mahoney <@t> alegent.org Mon Feb 28 11:56:10 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Feb 28 11:56:17 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8035D6976@EXCHANGECLUSTER.yumaregional.local> References: <4D6B6DAE.536C.000A.0@PCMH.COM> <010a01cbd76d$d57dce30$80796a90$@pathview.com> <29BE166A2CF48D459853F8EC57CD37E8035D6976@EXCHANGECLUSTER.yumaregional.local> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F2@EXCHMBC2.ad.ah.local> Histonet Users. Please help me understand something. I may be opening a big can of worms here but I'm confused. Why is it that Michael Mihalik is allowed to expound on the subject of workflow solutions when he is a competitor in that market? I'm not saying that some of his comments are not helpful but I thought this was a users forum? People usually freak out when a vendor tries to put comment on a competitors products, what is the difference here? I personally do not want to hear what he has to say here because he obviously has another agenda regardless of how it is disguised. I openly admit that I am a Ventana Vantage user and will stand by that system because I know it so well and know it's capabilities. The BIG difference is, I am a user, not a Vendor. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Monday, February 28, 2011 11:40 AM To: Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Well put Michael!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, February 28, 2011 10:35 AM To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From trathborne <@t> somerset-healthcare.com Mon Feb 28 12:02:49 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Feb 28 12:03:55 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F2@EXCHMBC2.ad.ah.local> Message-ID: I can say that I appreciated Michael's comments, but also think that he should have left his contact information and the company he represents off of the email. If anyone would have been interested in replying to him privately, they still could have done so. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Monday, February 28, 2011 12:56 PM To: 'Jesus Ellin'; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Histonet Users. Please help me understand something. I may be opening a big can of worms here but I'm confused. Why is it that Michael Mihalik is allowed to expound on the subject of workflow solutions when he is a competitor in that market? I'm not saying that some of his comments are not helpful but I thought this was a users forum? People usually freak out when a vendor tries to put comment on a competitors products, what is the difference here? I personally do not want to hear what he has to say here because he obviously has another agenda regardless of how it is disguised. I openly admit that I am a Ventana Vantage user and will stand by that system because I know it so well and know it's capabilities. The BIG difference is, I am a user, not a Vendor. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Monday, February 28, 2011 11:40 AM To: Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Well put Michael!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, February 28, 2011 10:35 AM To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. 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The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From JEllin <@t> yumaregional.org Mon Feb 28 12:08:42 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Feb 28 12:08:56 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F2@EXCHMBC2.ad.ah.local> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D697C@EXCHANGECLUSTER.yumaregional.local> Vendor's, whether we like it or not are going to monitor areas where we are expressing ideas and concerns. This cuts down on the need to do studies and customer data mining. I to think that vendors need to stay neutral, but the email clearly has a disclaimer,, so I think enough said on this one. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Monday, February 28, 2011 11:03 AM To: Mahoney,Janice A; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions I can say that I appreciated Michael's comments, but also think that he should have left his contact information and the company he represents off of the email. If anyone would have been interested in replying to him privately, they still could have done so. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Monday, February 28, 2011 12:56 PM To: 'Jesus Ellin'; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Histonet Users. Please help me understand something. I may be opening a big can of worms here but I'm confused. Why is it that Michael Mihalik is allowed to expound on the subject of workflow solutions when he is a competitor in that market? I'm not saying that some of his comments are not helpful but I thought this was a users forum? People usually freak out when a vendor tries to put comment on a competitors products, what is the difference here? I personally do not want to hear what he has to say here because he obviously has another agenda regardless of how it is disguised. I openly admit that I am a Ventana Vantage user and will stand by that system because I know it so well and know it's capabilities. The BIG difference is, I am a user, not a Vendor. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Monday, February 28, 2011 11:40 AM To: Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Well put Michael!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, February 28, 2011 10:35 AM To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. 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Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Janice.Mahoney <@t> alegent.org Mon Feb 28 12:09:55 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Feb 28 12:10:08 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F2@EXCHMBC2.ad.ah.local> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F5@EXCHMBC2.ad.ah.local> So you suggest that vendors pretend to be users by not stating who they are? I don't get it? I do not mean to be argumentative, I REALLY do not see how we are allowing this. Jan -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Monday, February 28, 2011 12:03 PM To: Mahoney,Janice A; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions I can say that I appreciated Michael's comments, but also think that he should have left his contact information and the company he represents off of the email. If anyone would have been interested in replying to him privately, they still could have done so. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Monday, February 28, 2011 12:56 PM To: 'Jesus Ellin'; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Histonet Users. Please help me understand something. I may be opening a big can of worms here but I'm confused. Why is it that Michael Mihalik is allowed to expound on the subject of workflow solutions when he is a competitor in that market? I'm not saying that some of his comments are not helpful but I thought this was a users forum? People usually freak out when a vendor tries to put comment on a competitors products, what is the difference here? I personally do not want to hear what he has to say here because he obviously has another agenda regardless of how it is disguised. I openly admit that I am a Ventana Vantage user and will stand by that system because I know it so well and know it's capabilities. The BIG difference is, I am a user, not a Vendor. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Monday, February 28, 2011 11:40 AM To: Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Well put Michael!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, February 28, 2011 10:35 AM To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. 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From PAMarcum <@t> uams.edu Mon Feb 28 12:12:21 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Feb 28 12:10:50 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F2@EXCHMBC2.ad.ah.local> Message-ID: I agree he should have left it off. However; we looked at Vantage and the cost for the interfaces and upgrades to be able to use it fully were very significant for us. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Monday, February 28, 2011 12:03 PM To: Mahoney,Janice A; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions I can say that I appreciated Michael's comments, but also think that he should have left his contact information and the company he represents off of the email. If anyone would have been interested in replying to him privately, they still could have done so. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Monday, February 28, 2011 12:56 PM To: 'Jesus Ellin'; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Histonet Users. Please help me understand something. I may be opening a big can of worms here but I'm confused. Why is it that Michael Mihalik is allowed to expound on the subject of workflow solutions when he is a competitor in that market? I'm not saying that some of his comments are not helpful but I thought this was a users forum? People usually freak out when a vendor tries to put comment on a competitors products, what is the difference here? I personally do not want to hear what he has to say here because he obviously has another agenda regardless of how it is disguised. I openly admit that I am a Ventana Vantage user and will stand by that system because I know it so well and know it's capabilities. The BIG difference is, I am a user, not a Vendor. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Monday, February 28, 2011 11:40 AM To: Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Well put Michael!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, February 28, 2011 10:35 AM To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. > You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From akbitting <@t> geisinger.edu Mon Feb 28 12:19:57 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Feb 28 12:20:08 2011 Subject: [Histonet] parts for CV5030 glass coverslipper In-Reply-To: References: <4D6B7F1E.2B7F.00C9.1@geisinger.edu> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB54CB@PHSXMB30.partners.org> Message-ID: <4D6BA0FD.2B7F.00C9.1@geisinger.edu> I'm not looking for repair parts. Just a place to order the filters that must be changed frequently. Sometimes, Fisher or VWR sell these type of consumables for Histo equipment. >>> Victoria Baker 2/28/2011 12:26 PM >>> Have you tried contacting Belair Instruments? I believe they are located in Fanwood NJ. Angie if you get a chance contact me off server :-). On Feb 28, 2011 11:04 AM, "Sherwood, Margaret" wrote: > We have a refurbished CV5030 coverslipper and we ordered directly from Leica. > If someone knows of anyone else who sells the parts/supplies, I would also be > interested. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting > Sent: Monday, February 28, 2011 10:55 AM > To: histonet > Subject: [Histonet] parts for CV5030 glass coverslipper > > Does anyone know if you can buy these from someone else besides Leica? My Fisher > rep never got back to me. > > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > IMPORTANT WARNING: The information in this message (and the documents attached > to it, if any) is confidential and may be legally privileged. It is intended > solely for the addressee. Access to this message by anyone else is unauthorized. > If you are not the intended recipient, any disclosure, copying, distribution or > any action taken, or omitted to be taken, in reliance on it is prohibited and > may be unlawful. If you have received this message in error, please delete all > electronic copies of this message (and the documents attached to it, if any), > destroy any hard copies you may have created and notify me immediately by > replying to this email. Thank you. > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Mon Feb 28 12:41:46 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Feb 28 12:41:57 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F5@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F2@EXCHMBC2.ad.ah.local> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F5@EXCHMBC2.ad.ah.local> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D697D@EXCHANGECLUSTER.yumaregional.local> I am coming from the stand point of an informed decision, there are a lot of other products out there other than a Vantage,, but it is the one with the most visibility currently. I do agree that Vendors do need to be neutral when giving perspective. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Monday, February 28, 2011 11:10 AM To: 'Rathborne, Toni'; Jesus Ellin; Michael Mihalik; CHRISTIEGOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions So you suggest that vendors pretend to be users by not stating who they are? I don't get it? I do not mean to be argumentative, I REALLY do not see how we are allowing this. Jan -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Monday, February 28, 2011 12:03 PM To: Mahoney,Janice A; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions I can say that I appreciated Michael's comments, but also think that he should have left his contact information and the company he represents off of the email. If anyone would have been interested in replying to him privately, they still could have done so. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Monday, February 28, 2011 12:56 PM To: 'Jesus Ellin'; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Histonet Users. Please help me understand something. I may be opening a big can of worms here but I'm confused. Why is it that Michael Mihalik is allowed to expound on the subject of workflow solutions when he is a competitor in that market? I'm not saying that some of his comments are not helpful but I thought this was a users forum? People usually freak out when a vendor tries to put comment on a competitors products, what is the difference here? I personally do not want to hear what he has to say here because he obviously has another agenda regardless of how it is disguised. I openly admit that I am a Ventana Vantage user and will stand by that system because I know it so well and know it's capabilities. The BIG difference is, I am a user, not a Vendor. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Monday, February 28, 2011 11:40 AM To: Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Well put Michael!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, February 28, 2011 10:35 AM To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From trathborne <@t> somerset-healthcare.com Mon Feb 28 12:48:57 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Feb 28 12:50:00 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F5@EXCHMBC2.ad.ah.local> Message-ID: I did not mean to imply that he should not have stated that he is a competitor, only that he leave off the last few lines which included a phone number and his employer. -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Monday, February 28, 2011 1:10 PM To: Rathborne, Toni; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions So you suggest that vendors pretend to be users by not stating who they are? I don't get it? I do not mean to be argumentative, I REALLY do not see how we are allowing this. Jan -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Monday, February 28, 2011 12:03 PM To: Mahoney,Janice A; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions I can say that I appreciated Michael's comments, but also think that he should have left his contact information and the company he represents off of the email. If anyone would have been interested in replying to him privately, they still could have done so. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Monday, February 28, 2011 12:56 PM To: 'Jesus Ellin'; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Histonet Users. Please help me understand something. I may be opening a big can of worms here but I'm confused. Why is it that Michael Mihalik is allowed to expound on the subject of workflow solutions when he is a competitor in that market? I'm not saying that some of his comments are not helpful but I thought this was a users forum? People usually freak out when a vendor tries to put comment on a competitors products, what is the difference here? I personally do not want to hear what he has to say here because he obviously has another agenda regardless of how it is disguised. I openly admit that I am a Ventana Vantage user and will stand by that system because I know it so well and know it's capabilities. The BIG difference is, I am a user, not a Vendor. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Monday, February 28, 2011 11:40 AM To: Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Well put Michael!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, February 28, 2011 10:35 AM To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Janice.Mahoney <@t> alegent.org Mon Feb 28 12:53:13 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Feb 28 12:53:23 2011 Subject: [Histonet] Histology Workflow Solutions In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F5@EXCHMBC2.ad.ah.local> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73FB@EXCHMBC2.ad.ah.local> Sorry for the misunderstanding. Jan -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Monday, February 28, 2011 12:49 PM To: Mahoney,Janice A; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions I did not mean to imply that he should not have stated that he is a competitor, only that he leave off the last few lines which included a phone number and his employer. -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Monday, February 28, 2011 1:10 PM To: Rathborne, Toni; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions So you suggest that vendors pretend to be users by not stating who they are? I don't get it? I do not mean to be argumentative, I REALLY do not see how we are allowing this. Jan -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Monday, February 28, 2011 12:03 PM To: Mahoney,Janice A; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions I can say that I appreciated Michael's comments, but also think that he should have left his contact information and the company he represents off of the email. If anyone would have been interested in replying to him privately, they still could have done so. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Monday, February 28, 2011 12:56 PM To: 'Jesus Ellin'; Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Histonet Users. Please help me understand something. I may be opening a big can of worms here but I'm confused. Why is it that Michael Mihalik is allowed to expound on the subject of workflow solutions when he is a competitor in that market? I'm not saying that some of his comments are not helpful but I thought this was a users forum? People usually freak out when a vendor tries to put comment on a competitors products, what is the difference here? I personally do not want to hear what he has to say here because he obviously has another agenda regardless of how it is disguised. I openly admit that I am a Ventana Vantage user and will stand by that system because I know it so well and know it's capabilities. The BIG difference is, I am a user, not a Vendor. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Monday, February 28, 2011 11:40 AM To: Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Well put Michael!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, February 28, 2011 10:35 AM To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions As many as you know this is a topic that is near and dear to my heart so I'd like to expound on this subject if you'll permit me. For those of you who already have solutions, or perhaps have more favored topics, please feel to move on to the next subject. First off, while I have some knowledge of both products, I am a user of neither. In fact I am a competitor of both, to some degree. Both products offer advantages and disadvantages. You really need to think about how they fit in your lab. When I say 'in your lab', I mean not only the histology area, but everyone, including the accessioning clerks, secretaries, pathologists, and even cytotechnologists. To me, one of the items that is more relevant to your situation is whether you want an integrated solution (IMPAC) or a standalone/interfaced solution. For instance, 1. How will information get into Vantage? Will it have to be manually accessioned/entered or will there be an interface? In the IMPAC solution this is a non issue. Also, keep in mind that there may be an interface cost from BOTH vendors should you decide an interface is in order. 2. How will information get OUT of Vantage or put another way, does the pathologist or secretary need to see information gathered/stored by Vantage? This may be resolved by another interface, but it may also be resolved by putting Vantage on more and more PCs. 3. Who is going to configure, manage and maintain Vantage? This is going to be a whole other computer system in your lab. Will your IT department support it, maintain it, administer it, etc. Who will understand and set up the files, etc.? You have the same issue with IMPAC, but I bet someone is already handling all these issues. 4. Can you use barcodes generated from Ventana on IMPAC? I don't know the answer to this question. It's not that each system can't read the barcode. It's a matter of whether IMPAC calls their slide, slide X and Vantage calls it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid and maybe Vantage calls it slideidNN. That means whenever the pathologist scans the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps this is not an issue, but I thought I would bring it up. 5. Management Reports: I know IMPAC supports Crystal Reports and at least theoretically you can build your own management reports. I don't what the situation with Vantage is. Are they canned reports or can you add more? Does this matter to you? I don't know enough about the details of either reporting module. Perhaps, the Vantage report subsystem generates everything you could possibly need and therefore why deal with Crystal Reports to generate unneeded reports. On the other hand, I note that in a response, Christie mentioned that Vantage tracked quality issues 'anywhere in the process'. I would wonder how Vantage could track quality issues at specimen receipt or at the pathologists PC if Vantage was not installed at those two areas. Perhaps as long as it's tracked somewhere, who cares what system contains the data. These are some serious issues to consider and as you might conclude, the analysis leans towards an integrated solution. However, that's not the whole story. There is really no magic to printing and scanning barcodes. The real science/magic lies in how those barcoded identifiers are used. You want them to make misidentification errors go away and you want them to constantly provide who/what/where information for any and all material. ...but again, HOW do the two sets of software do that? You might want to consider the following questions: 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? This is important because more PCs cost money and more PCs take up precious space. On the other hand, more PCs allow more ease of data entry. 2. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for IMPAC to display those images. What about patient history? Do you need to see that at grossing? 3. Do ALL slides print automatically when a block is scanned or do they have to be selected? You may have a preference here, but notice that I said 'slides for a block'. I can see both answers being 'correct'. 4. If you already have a Benchmark or Ultra, you have a built in motivation to use Vantage. With Vantage, you can use only ONE slide label to label slides going to those instruments. With IMPAC, you have to hand write or double label. This is because those 2 instruments require the use of Ventana's own label. Also, once the order for the benchmark or Ultra is in Vantage, I 'imagine' that this data would be sent to those instruments automatically. Please confirm this as I do not know if this is an additional cost. 5. Again, if you use either the Ultra or Benchmark, the anticipated slide completion date is available. This date/time is not available via an interface to IMPAC or any other vendor. 6. At grossing do you need to see the requisition? Are the requisitions scanned into IMPAC? That would make it difficult for Vantage to display those images. What about patient history? Do you need to see that at grossing? There are certainly other issues, and please don't get me wrong. I think that Vantage is a very sexy product. It looks very nice and it provides the positive ID everyone should really have. As you read the literature and get responses from others keep in mind that you have a choice a lot of people don't have. You can choose a system that is supported by your current vendor. A LOT of other people don't have that choice. It's either Vantage, a competitor to Vantage or a new LIS. You can get a lot of 'bang for the buck' with just Vantage. ...but if you're looking for a new LIS, well that's where my company comes in.... but that's a subject for a private email. P.S. The purpose of this email is to neither endorse one or the other product. My intention was to simply get people thinking about the issues involved, especially as tracking solutions and LIS's have evolved over the last few years. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Monday, February 28, 2011 7:05 AM To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Workflow Solutions Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome any comments on these systems and how they work in your lab or if you use something else. I would also welcome any suggestions or comments on how you label slides...Do you batch print and scan barcodes of the blocks and slides at the microtome? Do you scan blocks at the microtome and print labels there for the slides? Do you scan blocks at the microtome and print slides at the microtome? You may email directly if you prefer. Please share! Thanks in advance for your help and feedback! > > Dana > > ---------------------------------------------------------------------- > -------- The contents of this e-mail (and any attachments) are > confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ====================================================================== > ======== _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From shive003 <@t> umn.edu Mon Feb 28 13:05:40 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Feb 28 13:05:46 2011 Subject: [Histonet] Histology Workflow Solutions References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73F5@EXCHMBC2.ad.ah.local> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C73FB@EXCHMBC2.ad.ah.local> Message-ID: I think that if vendors contribute to a Histonet discussion, they should certainly let it be known that they're a vendor, and also who they work for. We need to be aware that their comments and opinions have a bias. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Mahoney,Janice A" To: "'Rathborne, Toni'" ; "Jesus Ellin" ; "Michael Mihalik" ; "CHRISTIE GOWAN" ; ; Sent: Monday, February 28, 2011 12:53 PM Subject: RE: [Histonet] Histology Workflow Solutions > Sorry for the misunderstanding. > Jan > > -----Original Message----- > From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] > Sent: Monday, February 28, 2011 12:49 PM > To: Mahoney,Janice A; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; > dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histology Workflow Solutions > > I did not mean to imply that he should not have stated that he is a > competitor, only that he leave off the last few lines which included a > phone number and his employer. > > -----Original Message----- > From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] > Sent: Monday, February 28, 2011 1:10 PM > To: Rathborne, Toni; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; > dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histology Workflow Solutions > > > So you suggest that vendors pretend to be users by not stating who they > are? I don't get it? > I do not mean to be argumentative, I REALLY do not see how we are allowing > this. > Jan > > -----Original Message----- > From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] > Sent: Monday, February 28, 2011 12:03 PM > To: Mahoney,Janice A; Jesus Ellin; Michael Mihalik; CHRISTIE GOWAN; > dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histology Workflow Solutions > > > I can say that I appreciated Michael's comments, but also think that he > should have left his contact information and the company he represents off > of the email. If anyone would have been interested in replying to him > privately, they still could have done so. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > Mahoney,Janice A > Sent: Monday, February 28, 2011 12:56 PM > To: 'Jesus Ellin'; Michael Mihalik; CHRISTIE GOWAN; > dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histology Workflow Solutions > > > Histonet Users. > Please help me understand something. I may be opening a big can of worms > here but I'm confused. > Why is it that Michael Mihalik is allowed to expound on the subject of > workflow solutions when he is a competitor in that market? I'm not saying > that some of his comments are not helpful but I thought this was a users > forum? People usually freak out when a vendor tries to put comment on a > competitors products, > what is the difference here? I personally do not want to hear what he has > to say here because he obviously has another agenda regardless of how it > is disguised. > I openly admit that I am a Ventana Vantage user and will stand by that > system because I know it so well and know it's capabilities. The BIG > difference is, I am a user, not a Vendor. > Jan > Omaha > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus > Ellin > Sent: Monday, February 28, 2011 11:40 AM > To: Michael Mihalik; CHRISTIE GOWAN; dana.spencer@pcmh.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histology Workflow Solutions > > Well put Michael!! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael > Mihalik > Sent: Monday, February 28, 2011 10:35 AM > To: 'CHRISTIE GOWAN'; dana.spencer@pcmh.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histology Workflow Solutions > > As many as you know this is a topic that is near and dear to my heart so > I'd > like to expound on this subject if you'll permit me. For those of you who > already have solutions, or perhaps have more favored topics, please feel > to > move on to the next subject. > > First off, while I have some knowledge of both products, I am a user of > neither. In fact I am a competitor of both, to some degree. Both > products > offer advantages and disadvantages. You really need to think about how > they > fit in your lab. When I say 'in your lab', I mean not only the histology > area, but everyone, including the accessioning clerks, secretaries, > pathologists, and even cytotechnologists. > > To me, one of the items that is more relevant to your situation is whether > you want an integrated solution (IMPAC) or a standalone/interfaced > solution. > For instance, > > 1. How will information get into Vantage? Will it have to be manually > accessioned/entered or will there be an interface? In the IMPAC solution > this is a non issue. Also, keep in mind that there may be an interface > cost > from BOTH vendors should you decide an interface is in order. > > 2. How will information get OUT of Vantage or put another way, does the > pathologist or secretary need to see information gathered/stored by > Vantage? > This may be resolved by another interface, but it may also be resolved by > putting Vantage on more and more PCs. > > 3. Who is going to configure, manage and maintain Vantage? This is going > to be a whole other computer system in your lab. Will your IT department > support it, maintain it, administer it, etc. Who will understand and set > up > the files, etc.? You have the same issue with IMPAC, but I bet someone is > already handling all these issues. > > 4. Can you use barcodes generated from Ventana on IMPAC? I don't know > the > answer to this question. It's not that each system can't read the > barcode. > It's a matter of whether IMPAC calls their slide, slide X and Vantage > calls > it 'y'. For instance, maybe IMPAC calls it case#.spnid.blockid.slideid > and > maybe Vantage calls it slideidNN. That means whenever the pathologist > scans > the slide at his IMPAC workstation, IMPAC won't recognize it. Perhaps > this > is not an issue, but I thought I would bring it up. > > 5. Management Reports: I know IMPAC supports Crystal Reports and at > least > theoretically you can build your own management reports. I don't what the > situation with Vantage is. Are they canned reports or can you add more? > Does this matter to you? I don't know enough about the details of either > reporting module. Perhaps, the Vantage report subsystem generates > everything you could possibly need and therefore why deal with Crystal > Reports to generate unneeded reports. On the other hand, I note that in a > response, Christie mentioned that Vantage tracked quality issues 'anywhere > in the process'. I would wonder how Vantage could track quality issues at > specimen receipt or at the pathologists PC if Vantage was not installed at > those two areas. Perhaps as long as it's tracked somewhere, who cares > what > system contains the data. > > These are some serious issues to consider and as you might conclude, the > analysis leans towards an integrated solution. However, that's not the > whole story. > > There is really no magic to printing and scanning barcodes. The real > science/magic lies in how those barcoded identifiers are used. You want > them to make misidentification errors go away and you want them to > constantly provide who/what/where information for any and all material. > ...but again, HOW do the two sets of software do that? > > You might want to consider the following questions: > > 1. Do you HAVE to use PCs for each grossing, embedding, cutting station? > This is important because more PCs cost money and more PCs take up > precious > space. On the other hand, more PCs allow more ease of data entry. > 2. At grossing do you need to see the requisition? Are the requisitions > scanned into IMPAC? That would make it difficult for IMPAC to display > those images. What about patient history? Do you need to see that at > grossing? > 3. Do ALL slides print automatically when a block is scanned or do they > have to be selected? You may have a preference here, but notice that I > said > 'slides for a block'. I can see both answers being 'correct'. > 4. If you already have a Benchmark or Ultra, you have a built in > motivation > to use Vantage. With Vantage, you can use only ONE slide label to label > slides going to those instruments. With IMPAC, you have to hand write or > double label. This is because those 2 instruments require the use of > Ventana's own label. Also, once the order for the benchmark or Ultra is > in > Vantage, I 'imagine' that this data would be sent to those instruments > automatically. Please confirm this as I do not know if this is an > additional cost. > 5. Again, if you use either the Ultra or Benchmark, the anticipated slide > completion date is available. This date/time is not available via an > interface to IMPAC or any other vendor. > 6. At grossing do you need to see the requisition? Are the requisitions > scanned into IMPAC? That would make it difficult for Vantage to display > those images. What about patient history? Do you need to see that at > grossing? > > There are certainly other issues, and please don't get me wrong. I think > that Vantage is a very sexy product. It looks very nice and it provides > the > positive ID everyone should really have. As you read the literature and > get > responses from others keep in mind that you have a choice a lot of people > don't have. You can choose a system that is supported by your current > vendor. A LOT of other people don't have that choice. It's either > Vantage, > a competitor to Vantage or a new LIS. You can get a lot of 'bang for the > buck' with just Vantage. > > ...but if you're looking for a new LIS, well that's where my company comes > in.... but that's a subject for a private email. > > > P.S. The purpose of this email is to neither endorse one or the other > product. My intention was to simply get people thinking about the issues > involved, especially as tracking solutions and LIS's have evolved over the > last few years. > > > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE > GOWAN > Sent: Monday, February 28, 2011 7:05 AM > To: dana.spencer@pcmh.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histology Workflow Solutions > > > Hi Dana, > I can't speak to the Elekta module but I have used the Vantage system for > over a year now. We generate a bar code at accessioning that is used > throughout the entire process to make cassettes, gross, embed, section, > stain and turn out. We do not batch during any of the process other than > the > 20 slides we put in our staining tray. We scan each block at microtomy as > we > section which prints a label that we affix to the slide when we pick up > the > section. We have virtually eliminated errors at all stations. We were able > to see quite an improvement in turn around times as well as tracking our > specimens. This system also allows us to track quality issues anywhere in > the process. Please feel free to contact me if you have further questions. > Christie Gowan > University of Alabama at Birmingham > Surgical Pathology > 205 934 4991 > >> Date: Mon, 28 Feb 2011 09:41:02 -0500 >> From: Dana.Spencer@PCMH.COM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Histology Workflow Solutions >> >> I am currently investigating workflow solutions for our Histology Lab > incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I > am looking into Elekta's AMP Module vs Ventana's Vantage system. I would > welcome any comments on these systems and how they work in your lab or if > you use something else. I would also welcome any suggestions or comments > on > how you label slides...Do you batch print and scan barcodes of the blocks > and slides at the microtome? Do you scan blocks at the microtome and print > labels there for the slides? Do you scan blocks at the microtome and print > slides at the microtome? You may email directly if you prefer. Please > share! > Thanks in advance for your help and feedback! >> >> Dana >> >> ---------------------------------------------------------------------- >> -------- The contents of this e-mail (and any attachments) are >> confidential, may be privileged and may contain copyright material. You > may only reproduce or distribute material if you are expressly authorized > by > us to do so. If you are not the intended recipient, any use, disclosure or > copying of this email (and any attachments) is unauthorized. 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Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tammy <@t> surgicalpathlabs.com Mon Feb 28 14:03:44 2011 From: tammy <@t> surgicalpathlabs.com (Tammy de Leon) Date: Mon Feb 28 14:05:59 2011 Subject: [Histonet] job openings Message-ID: If you're looking for a friendly environment, a new state-of-the-art LEED certified facility and an employer of choice, SPL is the place for you!! We are currently looking for qualified Histotechnicians/Histotechnologists and Lab Aides! Histo candidates should be an HTL or HT (ASCP) or equivalent. Primary responsibilities include on-site frozen sections including mobile laboratory units. SPL is CAP accredited, offers competitive pay, a comprehensive benefits package. SPL pays 100% of employee premiums for Medical, Dental, LTD and Life. We also offer Retirement Plan & Supplemental Insurance. Please forward resume to Tammy de Leon tammy@surgicalpathlabs.com Surgical Pathology Laboratory 800-304-1066 8455 66th Street N Pinellas Park, Florida 33781 www.surgicalpathlabs.com From Cody.Lashley <@t> bms.com Mon Feb 28 14:08:11 2011 From: Cody.Lashley <@t> bms.com (Lashley, Cody) Date: Mon Feb 28 14:08:16 2011 Subject: [Histonet] (no subject) Message-ID: Hi, We are going to be doing a special project on a rooster with a large puss pocket under the epiglottis/esophagus-trachea area, about an inch long. The question I have, is there a need to decalcify this area to facilitate longitudinal sectioning? If so are there any recommendations on decal times and types of decal to use? We have never worked with fowl before so any help would be appreciated. Thanks Cody ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From melissa.ribeiro <@t> brinegroup.com Mon Feb 28 14:34:14 2011 From: melissa.ribeiro <@t> brinegroup.com (Melissa Ribeiro Passos) Date: Mon Feb 28 14:34:28 2011 Subject: [Histonet] Histology & Cytology Manager opportunity, south of Boston Message-ID: <43904A2EECEAB54D8A023931049FEA4C528592@brin-sbs01.brinegroup.local> Brine Group is conducting a search for a talented Histology/Cytology professional for the following leadership role: Pathology Manager (Histology & Cytology) Magnet community hospital south of Boston scoring above state, regional, and national averages in overall patient satisfaction Press-Ganey's scores has an opening for a Pathology Manager to oversee the technical & clinical aspects of a 21-person department including Histology, Surgical Pathology, Cytology and Support Services. Qualifications: * Minimum Associate's Degree in Biological Science or equivalent educational/technical training as a Histology Technician preferred (bachelor's preferred) * Minimum five (5) years progressive experience as a Histotechnologist. * Minimum two (2) years experience as a Histology Supervisor. * HTL or HT (ASCP) certification required. If this position describes you or someone you know, please contact Melissa Ribeiro Passos for additional information - 781.272.3400 ext.228 / mribeiro@brinegroup.com If you are currently working with another Brine Group consultant, please feel free to contact him/her directly. Melissa Ribeiro Passos Partner Brine Group Staffing Solutions Healthcare Division 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph. (781) 272-3400 ext. 228 Cell (978) 397-4300 Fax (781) 494-3401 From histotech411 <@t> gmail.com Mon Feb 28 16:18:37 2011 From: histotech411 <@t> gmail.com (Jenny Vega) Date: Mon Feb 28 16:18:41 2011 Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? Message-ID: I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks From ruppert.amysue <@t> marshfieldclinic.org Mon Feb 28 23:25:50 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Mon Feb 28 23:25:55 2011 Subject: [Histonet] Poor nuclear artifact&In-Reply-To= Message-ID: <201103010525.p215Phlf025838@spamfilt> Are the individual pieces that have the staining problem larger than the rest of the pieces in the same block? What type of tissue is this happening with? Is it happening with tissues that are coming from one particular account? It seems that if the staining issue is not across the board with all your tissues or even with tissue pieces in the same block, than you need to look at some of the above questions. Tissue such as a skin tag may seem small enough for a bx run, but in reality, tissue like this is sort of like a basketball.... The tough outer skin does not allow for the processing solutions to pass through easily and penetrate as it should. Even a large skin tag or colon polyp that is bisected may have this happen on those pieces that are like half a basketball...solutions get trapped inside, but cannot penetrate pass the outer shell. And so these pieces end up improperly processed. Or, if this is happening with a particular account, could something have changed there..new pe rsonnel that may not know the importance of proper fixation, and are individual tissue pieces drying out before being placed into the fixative? You mentioned Alaska, could any of these possibly have become frozen or partially frozen in transport? good luck AmySue R. ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation.