From jkiernan <@t> uwo.ca Thu Dec 1 00:39:06 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Dec 1 00:39:11 2011 Subject: [Histonet] Info for all Re: nuclear fast red In-Reply-To: <7660b19412790.4ed720e3@uwo.ca> References: <20111130180428.0FA3E13D2A5A@barracuda.crvinc.net> <005501ccaf96$4bc35730$e001a8c0@cdd.loc> <75b0ba7c12b2a.4ed71ebc@uwo.ca> <75b08e8016718.4ed71ef8@uwo.ca> <76a0995713b28.4ed71f73@uwo.ca> <74009dca1628d.4ed71fb0@uwo.ca> <75c090d6131f8.4ed71fed@uwo.ca> <75c0a1f0151f3.4ed7202b@uwo.ca> <75c0a3781133c.4ed72068@uwo.ca> <76a0a7dd15f3f.4ed720a6@uwo.ca> <7660b19412790.4ed720e3@uwo.ca> Message-ID: <74f08b9213599.4ed6daba@uwo.ca> Nuclear fast red was added to the Biological Stain Commission's list of Certified Stains 4 or 5 years ago. http://biostain.com Buy from a vendor who sells NFR from a Certified batch. Think before buying anything else. John Kiernan Secretary, Biological Stain Commission = = = On 30/11/11, Tim Higgins wrote: > > Nuclear Fast Red needs to be heated during prep and it does take a little > while to dissolve, it never really dissolves entirely, probably saturates > the solution pretty fast. > > Tim > > Message: 1 > Date: Tue, 29 Nov 2011 11:29:07 -0700 > From: "Patsy Ruegg" > Subject: [Histonet] nuclear fast red > To: > Message-ID: <94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice> > Content-Type: text/plain; charset="us-ascii" > > Is my nuclear fast red powder dead or does it take a long time with heat and > stirring to turn red? It is pretty old, and one vial says it should be > stored below 0 but has not been. > > > > Cheers, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email > pruegg@ihctech.net > web site www.ihctech.net > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From abilger <@t> wellspan.org Thu Dec 1 07:28:38 2011 From: abilger <@t> wellspan.org (Bilger, Andrea) Date: Thu Dec 1 07:29:31 2011 Subject: [Histonet] Fresh specimens after hours Message-ID: <6D7752544B308D44A902C0BD0EC7BF5C8C5991A2@EXCH02.wellspan.org> We are not staffed 24/7. The OR here has a supply of small pre-filled formalin containers that they actually take into the OR room but not in the sterile area. They put the specimens directly in them and then, at the end of the procedure, deliver them just outside our Frozen Section room for us to pick up numerous times a day. Larger specimens are brought fresh to a refrigerator outside our Frozen room. We will pick them up and put formalin on them when we are here. At the end of the day, a sign goes on the refrigerator door that Pathology is closed and no fresh specimens can be placed in the frig. We have a supply of empty large containers in our Frozen room that the OR staff grab place in the Frozen Room sink and place the specimen in it. They then pour formalin on the specimen and carry it next door to the specimen refrigerator. Now all we have to do is get the OR staff to record the ischemic time somewhere. Andrea Bilger Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From LRaff <@t> uropartners.com Thu Dec 1 08:33:23 2011 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Dec 1 08:33:29 2011 Subject: [Histonet] Fresh specimens after hours In-Reply-To: <6D7752544B308D44A902C0BD0EC7BF5C8C5991A2@EXCH02.wellspan.org> References: <6D7752544B308D44A902C0BD0EC7BF5C8C5991A2@EXCH02.wellspan.org> Message-ID: Just be sure there is a log book or computer entry system for the OR to log in the fact that they are leaving a specimen. Otherwise, specimens can disappear with the lab totally unaware. It happened to us in my previous position. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bilger, Andrea Sent: Thursday, December 01, 2011 7:29 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Fresh specimens after hours We are not staffed 24/7. The OR here has a supply of small pre-filled formalin containers that they actually take into the OR room but not in the sterile area. They put the specimens directly in them and then, at the end of the procedure, deliver them just outside our Frozen Section room for us to pick up numerous times a day. Larger specimens are brought fresh to a refrigerator outside our Frozen room. We will pick them up and put formalin on them when we are here. At the end of the day, a sign goes on the refrigerator door that Pathology is closed and no fresh specimens can be placed in the frig. We have a supply of empty large containers in our Frozen room that the OR staff grab place in the Frozen Room sink and place the specimen in it. They then pour formalin on the specimen and carry it next door to the specimen refrigerator. Now all we have to do is get the OR staff to record the ischemic time somewhere. Andrea Bilger Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Thu Dec 1 08:45:39 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Dec 1 08:44:35 2011 Subject: [Histonet] SOP for validation In-Reply-To: References: <6D7752544B308D44A902C0BD0EC7BF5C8C5991A2@EXCH02.wellspan.org> Message-ID: <5A33C952BB67F4468AF1F36D739212BC06473A@JERRY.Gia.com> Would anyone be willing to share their SOP for validation? Thanks! From sbreeden <@t> nmda.nmsu.edu Thu Dec 1 08:53:58 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Dec 1 08:54:07 2011 Subject: [Histonet] Cost of 10% NBF Message-ID: <02C099024072804EA34F5906BAC30A41062BBC@nmdamailsvr.nmda.ad.nmsu.edu> I don't suppose that anyone out in HistoPersonLand has done a cost comparison between making your own 10% NBF and purchasing a 50-gallon drum of 10% NBF, perhaps? I did this several years ago but my idea of saving tech time/costs by buying the bulk was shot down because there was someone here that did the making. Now we're even more shorthanded and I think it would be a better use of everyone's time to just buy the darned stuff in bulk. Any chances that someone's already done this? If you have, I'd appreciate your input and my everlasting gratitude (brownies not included). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From rjbuesa <@t> yahoo.com Thu Dec 1 09:03:15 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 1 09:03:22 2011 Subject: [Histonet] Cost of 10% NBF In-Reply-To: <02C099024072804EA34F5906BAC30A41062BBC@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1322751795.60319.YahooMailClassic@web65708.mail.ac4.yahoo.com> Regardless of any economic consideration, NBF should NEVER be prepared in the lab. The exposure of the personnel is too high and dangerous and ought to prevail over costs savings. Many, many years ago we also prepared our own NBF but abandoned that practice also many many?years ago. Buy the NBF that is prepared under better conditions that any you could try to reproduce in your lab. Ren? J. --- On Thu, 12/1/11, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Cost of 10% NBF To: histonet@lists.utsouthwestern.edu Date: Thursday, December 1, 2011, 9:53 AM I don't suppose that anyone out in HistoPersonLand has done a cost comparison between making your own 10% NBF and purchasing a 50-gallon drum of 10% NBF, perhaps?? I did this several years ago but my idea of saving tech time/costs by buying the bulk was shot down because there was someone here that did the making.? Now we're even more shorthanded and I think it would be a better use of everyone's time to just buy the darned stuff in bulk.? Any chances that someone's already done this?? If you have, I'd appreciate your input and my everlasting gratitude (brownies not included). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From silvinamolinuevo <@t> yahoo.com.ar Thu Dec 1 08:25:00 2011 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Thu Dec 1 09:19:34 2011 Subject: [Histonet] b-cells stain Message-ID: <1322749500.19574.YahooMailNeo@web113609.mail.gq1.yahoo.com> Hi Histonetters!! I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin? I know that the best stain is?Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions about that product in my country. Thanks in advance. ? Dr Mar?a Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina www.biol.unlp.edu.ar/giomm e-mail: silvina.molinuevo@bigfoot.com From Lynn.Burton <@t> Illinois.gov Thu Dec 1 09:20:30 2011 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Dec 1 09:23:40 2011 Subject: [Histonet] RE: Cost of 10% NBF In-Reply-To: <02C099024072804EA34F5906BAC30A41062BBC@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062BBC@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00645680BCD@IL084EXMBX214.illinois.gov> Sorry Sara, We make our own here too. I can't remember why exactly. One reason is the space for a 50 gallon drum I believe. Good Luck. Bureaucrats always think personnel is cheap enough to be expendable. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Thursday, December 01, 2011 8:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost of 10% NBF I don't suppose that anyone out in HistoPersonLand has done a cost comparison between making your own 10% NBF and purchasing a 50-gallon drum of 10% NBF, perhaps? I did this several years ago but my idea of saving tech time/costs by buying the bulk was shot down because there was someone here that did the making. Now we're even more shorthanded and I think it would be a better use of everyone's time to just buy the darned stuff in bulk. Any chances that someone's already done this? If you have, I'd appreciate your input and my everlasting gratitude (brownies not included). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhawkins <@t> UTMB.EDU Thu Dec 1 09:55:02 2011 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Thu Dec 1 09:55:19 2011 Subject: [Histonet] FW: EM questions In-Reply-To: <38EBF84C289C41E8A822243B6C5FF109@Patsyoffice> References: <38EBF84C289C41E8A822243B6C5FF109@Patsyoffice> Message-ID: <22624908330375439D6382C9F95093FF68B84A@GRMBX1.utmb.edu> I would suggest using some sort of conductive adhesive for what I guess is analysis of the minerals by scanning electron microscopy and energy-dispersive X-ray analysis of their elemental composition. I would think that embedding in a high melting point wax might have adverse effects by absorbing/scattering the electron beam in the high-carbon wax before it penetrated to the mineral grains. Check out these products: http://www.tedpella.com/SEMmisc_html/SEMpaint.htm ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Patsy Ruegg [pruegg@ihctech.net] Sent: Wednesday, November 30, 2011 5:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: EM questions Can anyone help with this, I haven't got a clue? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Suarez, Andrea Luisa [mailto:ANDREA.SUAREZ@UCDENVER.EDU] Sent: Wednesday, November 30, 2011 3:22 PM To: pruegg@ihctech.net Cc: High, Whitney Subject: EM questions Hi Patsy, Not sure if you remember me from my days as a graduate student in the van Dyk lab. How are you? I am working w/ Dr Whit High at CU dermpath on an EM project. I had some questions that perhaps you might have some answers to. We are doing a project where we will be doing QEMSCAN at school of mines, which has the capacity to do a very sophisticated analysis of mineral composition. We will be looking for volcanic ash deposition in human skin samples, and would like to embedd the tissues in as pure a fashion as possible, with optimum antigen preservation. We have some concerns about parrafin embedding, as we plan to mount the sections onto carbon planchets. Our experience w/ this in the past, is that adherence to the planchet is poor after deparafinizaiton. Therefore, we would like to optimize adherence, if possible, or avoid the need for deparafinization by embedding in a wax (such as carnuba) w/ a very high melting temp. 1.) Do you have any experience/insight regarding embedding in carnuba wax? Do you know of anyone who would be willing to do this for us? 2.) Do you have any recomendations as to how to improve adherence to the carbon planchet in the event that we do end up having to de-parafinize? We were thinking that adding albumin to the water bath may help things. Any thoughts on this? Many thanks and I look forward to hearing from you. Sincerely, Andrea Suarez, MD/PhD Colorado Health Foundation Transitional Intern andrea.suarez@ucdenver.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kaitlin <@t> prometheushealthcare.com Thu Dec 1 10:15:59 2011 From: kaitlin <@t> prometheushealthcare.com (Kaitlin Webster) Date: Thu Dec 1 10:16:09 2011 Subject: [Histonet] Upper Level Histology Positions Message-ID: <006501ccb044$83bcb970$8b362c50$@prometheushealthcare.com> I am a nationwide laboratory recruiter and I have several histology supervisor and manager level positions located nationwide. All positions offer great salaries and relocation. For more info contact me, Kaitlin at 301.693.9057. Kaitlin Webster Account Manager Prometheus Healthcare Office (301) 693-9057 Cell (407) 334-4438 Fax (301) 368-2478 Kaitlin@prometheushealthcare.com www.prometheushealthcare.com From kcai <@t> prosci-inc.com Thu Dec 1 12:01:31 2011 From: kcai <@t> prosci-inc.com (Karen Cai) Date: Thu Dec 1 12:01:34 2011 Subject: [Histonet] Rabbit antibody on rabbit tissue Message-ID: <002e01ccb053$419045d0$c4b0d170$@com> Hi All, I am going to apply one rabbit polyclonal antibody on the rabbit tissue using IF. Is there anybody do it before? I heard of mouse on mouse IHC-DAB method, but not sure if it's possible in IF. Your kind response is very appreciated, Thanks, Karen From mcauliff <@t> umdnj.edu Thu Dec 1 12:18:40 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Dec 1 12:17:49 2011 Subject: [Histonet] b-cells stain In-Reply-To: <1322749500.19574.YahooMailNeo@web113609.mail.gq1.yahoo.com> References: <1322749500.19574.YahooMailNeo@web113609.mail.gq1.yahoo.com> Message-ID: <4ED7C500.70805@umdnj.edu> Greetings Silvina: You do not have to use paraldehyde, you can make a perfectly good aldehyde fuchsin using acetaldehyde since one molecule of paraldehyde depolymerizes into 3 molecules of acetaldehyde. Just use 3x the amount of acetaldehyde. n-butyraldehyde will also work for b-cells. See Buehner et al. J.Histochem. Cytochem. 27(3):782787, 1979 for more thorough discussion of the chemistry. And, if a ferrous hematoxylin worked for b-cells we would not need the chrome alum variety. Geoff On 12/1/2011 9:25 AM, Silvina Molinuevo wrote: > Hi Histonetters!! > I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin? > I know that the best stain is Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions about that product in my country. > Thanks in advance. > > Dr Mar?a Silvina Molinuevo > Grupo de Investigacion en > Osteopatias y Metabolismo Mineral > Departamento de Ciencias Biologicas > Facultad de Ciencias Exactas > Universidad Nacional de La Plata > 47 y 115 > (1900)La Plata > Argentina > www.biol.unlp.edu.ar/giomm > e-mail: silvina.molinuevo@bigfoot.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From SWeaver <@t> tvmdl.tamu.edu Thu Dec 1 12:18:01 2011 From: SWeaver <@t> tvmdl.tamu.edu (Weaver, Stephanie) Date: Thu Dec 1 12:18:00 2011 Subject: [Histonet] identification of Protheca Message-ID: Dear Histonetters: One of my pathologists is seeking a method to identify and speciate Prototheca in fixed animal tissues. I can't seem to find anyone that does this. Any ideas? Stephanie Weaver Texas Veterinary Medical Diagnostic Laboratory From trathborne <@t> somerset-healthcare.com Thu Dec 1 14:54:26 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Dec 1 14:54:53 2011 Subject: [Histonet] Cost of 10% NBF In-Reply-To: <1322751795.60319.YahooMailClassic@web65708.mail.ac4.yahoo.com> References: <02C099024072804EA34F5906BAC30A41062BBC@nmdamailsvr.nmda.ad.nmsu.edu> <1322751795.60319.YahooMailClassic@web65708.mail.ac4.yahoo.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F64439@SMCMAIL01.somerset-healthcare.com> I agree with Rene'. Many years ago at a previous facility, I was wearing all of my PPE's, including a full-face respirator, gloves, and jumpsuit while making 10% nbf. However, the 37% formalin (which was being pumped in with a hose) splashed on my protective jumpsuit. The incident caused prolonged respiratory problems. It is not something that should be done in a lab. Buy it ready to use, and in a size that is easy enough for your staff to handle safely. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 01, 2011 10:03 AM To: histonet@lists.utsouthwestern.edu; SaraBreeden Subject: Re: [Histonet] Cost of 10% NBF Regardless of any economic consideration, NBF should NEVER be prepared in the lab. The exposure of the personnel is too high and dangerous and ought to prevail over costs savings. Many, many years ago we also prepared our own NBF but abandoned that practice also many many?years ago. Buy the NBF that is prepared under better conditions that any you could try to reproduce in your lab. Ren? J. --- On Thu, 12/1/11, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Cost of 10% NBF To: histonet@lists.utsouthwestern.edu Date: Thursday, December 1, 2011, 9:53 AM I don't suppose that anyone out in HistoPersonLand has done a cost comparison between making your own 10% NBF and purchasing a 50-gallon drum of 10% NBF, perhaps?? I did this several years ago but my idea of saving tech time/costs by buying the bulk was shot down because there was someone here that did the making.? Now we're even more shorthanded and I think it would be a better use of everyone's time to just buy the darned stuff in bulk.? Any chances that someone's already done this?? If you have, I'd appreciate your input and my everlasting gratitude (brownies not included). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From sbreeden <@t> nmda.nmsu.edu Thu Dec 1 15:44:26 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Dec 1 15:44:32 2011 Subject: [Histonet] Formalin Pricing Follow-up Message-ID: <02C099024072804EA34F5906BAC30A41062BCE@nmdamailsvr.nmda.ad.nmsu.edu> Thanks to everyone who gave me Peer Review on my make-formalin-vs-buy-formalin. I appreciate everyone's input and with all the comments, I think I've convinced Our Fearless Leader to go with the 55-gallon drum idea. Thank you, everyone! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From amosbrooks <@t> gmail.com Thu Dec 1 15:59:36 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Dec 1 15:59:43 2011 Subject: [Histonet] b-cells stain Message-ID: Hi, I like to prepare solutions myself as well. As you say though paraldehyde is classified as a drug so there are hoops to jump through to be able to purchase it. This is one of those times that it totally makes sense to let someone else make it up. EMS sells aldehyde fuchsin so it saves me the hassle of dealing with the DEA. Better yet the price isn't terrible and works great. Here's the info for it: RT 26096 Aldehyde Fuchsin Solution 250 ml 24.00 http://www.emsdiasum.com/microscopy/products/histology/staining_solutions.aspx#26091 Now as for Gomori's chrome alum hematoxylin, you really are better off using the chrome (potassium dichromate if I recall correctly) than ferric chloride (Weigerts formulation right?). I don't think the iron hematoxylin would be as specific to the beta cells, but hey, if you decide to try it, please let me know if it works out for you. Ferric chloride is better to work with than potassium dichromate! Heck you could write up a new procedure and name it after yourself. Amos On Thu, Dec 1, 2011 at 1:00 PM, wrote: > Message: 3 > Date: Thu, 1 Dec 2011 06:25:00 -0800 (PST) > From: Silvina Molinuevo > Subject: [Histonet] b-cells stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1322749500.19574.YahooMailNeo@web113609.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi Histonetters!! > I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. > Does anybody know if the stain go well with ferrum hematoxylin instead of > chrome hematoxylin? > I know that the best stain is Aldehyde-fuchsin but I can't import > paraldehyde because of legal restrictions about that product in my country. > Thanks in advance. > > Dr Mar?a Silvina Molinuevo > Grupo de Investigacion en > Osteopatias y Metabolismo Mineral > Departamento de Ciencias Biologicas > Facultad de Ciencias Exactas > Universidad Nacional de La Plata > 47 y 115 > (1900)La Plata > Argentina > From brett_connolly <@t> merck.com Thu Dec 1 16:11:53 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Dec 1 16:11:59 2011 Subject: [Histonet] pan-cathepsin antibody Message-ID: Hi all- I'm looking for a pan-cathepsin antibody for FFPE IHC and can only find Santa Cruz sc-6499. Has anyone used this, or other pan cathepsin antibodies? Thanks, Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From melissa.ribeiro <@t> brinegroup.com Thu Dec 1 16:52:16 2011 From: melissa.ribeiro <@t> brinegroup.com (Melissa Ribeiro Passos) Date: Thu Dec 1 16:52:23 2011 Subject: [Histonet] Day shift, histotech opportunity, Connecticut Message-ID: <828AD65097415F4ABADAC27511EDE3AC0A44@brin-sbs01.brinegroup.local> HISTOTECH, DAY SHIFT OPPORTUNITY Brine Group is recruiting around a Histology Technologist / Technician position in Connecticut. (Day shift) This is a clinical/surgical pathology hospital-based lab equipped with "cutting edge" instrumentation and information management technology. Responsible for preparing slides from surgical and autopsy materials for examination by pathologist for diagnosis, this position will have the following unit specific responsibilities: * Load surgical specimens in Autotechnicon and sets/checks time setting. * Check identification and prepares surgical specimens for gross examination by pathologist. * Maintains positive specimen identification throughout specimen processing. * Cut embedded surgical specimens using rotary microtome. * Cut sections and performs H&E staining. * Perform various special stains. * Examine specimens to determine that they meet established standards. * Coordinate slides and pathology reports, and takes to pathologist for diagnosis. * Prepare and maintains reagents, solutions and stains, according to SOP's. REQUIREMENTS * Routine Histology and Special Stains experience from a clinical/surgical or reference lab (no research). * HT or HTL (ASCP) certification is a must. * Bachelor's degree from an accredited college / university with appropriate semester hours in biology and chemistry. * Formal histology training in an approved, structured program. INTERESTED? Contact Melissa Ribeiro Passos at: 781.272.3400 ext.228 or melissa.ribeiro@brinegroup.com Melissa Ribeiro Passos Partner Brine Group Staffing Solutions Healthcare Division 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph. (781) 272-3400 ext. 228 Fax (781) 494-3401 From lpwenk <@t> sbcglobal.net Thu Dec 1 21:49:31 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Dec 1 21:49:35 2011 Subject: [Histonet] b-cells stain In-Reply-To: <1322749500.19574.YahooMailNeo@web113609.mail.gq1.yahoo.com> References: <1322749500.19574.YahooMailNeo@web113609.mail.gq1.yahoo.com> Message-ID: <8B4D82F448A84BB58A8117D457A91D56@HP2010> Acetaldehyde can be used instead of paraldehyde, to make aldehyde fuchsin. Substitute 1.5 - 2.0 mL acetaldehyde for every 1.0 mL of paraldehyde. Acetaldehyde is usually not a restricted drug, a whole lot cheaper than acetaldehyde, and once opened, remains good for much longer than paraldehyde, when stored in the refrigerator (at least 18 months instead for the 2-3 months for paraldehyde). The aldehyde-fuchsin, once made, will only be good for about 3-4 weeks, when stored in the refrig, regardless of whether it was made with paraldehyde or acetaldehyde. The source for the exchange was this article: Gabe, M. 1953. Sur quelques applications de la coloration par la fuchsine-paraldehyde. Bull. Micros. Appl. Ser. 2, 3: 153-162. One of my students, Yelena Mushkina, HTL(ASCP), translated it from the French to her native Russian, and then from Russian to English. Took a couple of dictionaries! I wrote this up in a Letter to the Editor in the Dec. 1996 NSH Journal of Histotechnology "Acetaldehyde as a Substitute for Paraldehyde". In case you need some references. IHC is actually better for demonstrating beta cells - whether for pituitary or for pancreas. Just something else to think about. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Silvina Molinuevo Sent: Thursday, December 01, 2011 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] b-cells stain Hi Histonetters!! I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin? I know that the best stain is Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions about that product in my country. Thanks in advance. Dr Mar?a Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina www.biol.unlp.edu.ar/giomm e-mail: silvina.molinuevo@bigfoot.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Dec 2 01:20:11 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Dec 2 01:20:20 2011 Subject: [Histonet] identification of Protheca In-Reply-To: <761096125bc9.4ed87c0f@uwo.ca> References: <7690bbca123e.4ed879e7@uwo.ca> <76908afb368e.4ed87a24@uwo.ca> <7690c0aa24fc.4ed87a61@uwo.ca> <74b08edf3d0.4ed87a9e@uwo.ca> <74b0a1fd3c10.4ed87adc@uwo.ca> <765085ce90c.4ed87b19@uwo.ca> <7650f4b06f4a.4ed87b56@uwo.ca> <75c0c0a0658e.4ed87b94@uwo.ca> <7650a8fb3e7b.4ed87bd1@uwo.ca> <761096125bc9.4ed87c0f@uwo.ca> Message-ID: <76109d93561f.4ed835db@uwo.ca> One minute with Google turned up this reference. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC380951/pdf/applmicro00058-0151.pdf It will have been cited by many other papers. Your pathologist boss should be able to find them on a cited reference search web site. The best ones are Web of Science and Scopus, available through libraries. Google Scholar (free for anyone) is also good, but it's slower, less organized, and may show local items with ads at the top of the heap and the good stuff a bit further down. Probably there are programs for veterinary literature searches. A veterinary pathologist should know. John Kiernan Anatomy, UWO London, Canada = = = On 01/12/11, "Weaver, Stephanie" wrote: > > Dear Histonetters: > > One of my pathologists is seeking a method to identify and speciate Prototheca in fixed animal tissues. I can't seem to find anyone that does this. Any ideas? > > Stephanie Weaver > Texas Veterinary Medical Diagnostic Laboratory > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From amosbrooks <@t> gmail.com Fri Dec 2 06:01:10 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Dec 2 06:01:20 2011 Subject: [Histonet] b-cells stain In-Reply-To: <7690b605365f.4ed83225@uwo.ca> References: <74f0a636627.4ed87761@uwo.ca> <7550e88e2a40.4ed8779e@uwo.ca> <7660d9d8156.4ed877dc@uwo.ca> <76908ef66e08.4ed87855@uwo.ca> <7690b605365f.4ed83225@uwo.ca> Message-ID: Thanks John, Since I was writing the message about Gomori's chrome alum hematoxylin without the procedure in front of me yesterday (not the best idea, I know) I didn't have the whole formulation available to me. For Gomori's chrome alum hematoxylin, in addition to potassium dichromate, there is also chromium potassium sulfate and sulfuric acid as well. By no means is there any ferric chloride in it and as I mentioned if one tried to make this with ferric chloride, I doubt it would work for beta cells since according to Bancroft in *Theory & Practice of Histotechnology* chrome alum has a strong affinity for sulfonic acid radicals which are produced in the oxidation of the glycoproteins in the beta cells in earlier steps of the stain. So long story short: ferric chloride probably would not work for Gomori's stain. Sorry if it sounded like I suggested it would. I really appreciate having folks like John Kiernan around to point out possible mis-communications like that. Have a great weekend everyone, Amos On Fri, Dec 2, 2011 at 2:04 AM, John Kiernan wrote: > Dear Amos, > You may have confused some histonetters if any of them try to make up > Gomori's chrome alum hematoxylin with either potassium dichromate or ferric > chloride! > Cheers, > John Kiernan > Anatomy, UWO > London, Canada > = = = > On 01/12/11, *Amos Brooks * wrote: > > Hi, > I like to prepare solutions myself as well. As you say though > paraldehyde is classified as a drug so there are hoops to jump through to > be able to purchase it. This is one of those times that it totally makes > sense to let someone else make it up. EMS sells aldehyde fuchsin so it > saves me the hassle of dealing with the DEA. Better yet the price isn't > terrible and works great. Here's the info for it: > RT 26096 Aldehyde Fuchsin Solution 250 ml 24.00 > > http://www.emsdiasum.com/microscopy/products/histology/staining_solutions.aspx#26091 > Now as for Gomori's chrome alum hematoxylin, you really are better off > using the chrome (potassium dichromate if I recall correctly) than ferric > chloride (Weigerts formulation right?). I don't think the iron hematoxylin > would be as specific to the beta cells, but hey, if you decide to try it, > please let me know if it works out for you. Ferric chloride is better to > work with than potassium dichromate! Heck you could write up a new > procedure and name it after yourself. > > Amos > > > On Thu, Dec 1, 2011 at 1:00 PM, > wrote: > > > Message: 3 > > Date: Thu, 1 Dec 2011 06:25:00 -0800 (PST) > > From: Silvina Molinuevo > > Subject: [Histonet] b-cells stain > > To: "histonet@lists.utsouthwestern.edu" > > > > Message-ID: > > <1322749500.19574.YahooMailNeo@web113609.mail.gq1.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > Hi Histonetters!! > > I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. > > Does anybody know if the stain go well with ferrum hematoxylin instead of > > chrome hematoxylin? > > I know that the best stain is Aldehyde-fuchsin but I can't import > > paraldehyde because of legal restrictions about that product in my > country. > > Thanks in advance. > > > > Dr Mar?a Silvina Molinuevo > > Grupo de Investigacion en > > Osteopatias y Metabolismo Mineral > > Departamento de Ciencias Biologicas > > Facultad de Ciencias Exactas > > Universidad Nacional de La Plata > > 47 y 115 > > (1900)La Plata > > Argentina > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From PAMarcum <@t> uams.edu Fri Dec 2 09:06:30 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Dec 2 09:16:40 2011 Subject: [Histonet] UAMS Little Rock for HT or HTL Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3215BF9DB4@Mail2Node2.ad.uams.edu> Good Morning, We are looking for a register Histologist for a fulltime job at the University of Arkansas for Medical Sciences in Little Rock. This is a chance to learn and grow with the Histology department at UAMS. The benefits are great and the retirement is a matching up to 10% for your contributions by UAMS. We have 11 paid holidays each year and two weeks of vacation with sick time allotted per month. We cannot pay relocation or bonuses as this is a state run University site and we cannot use a recruiter, so please do not ask. I have tried and it is no recruiters. Please see the UAMS website for Jobs under #5003810 and submit a resume. You may also call me for details or other information however: I cannot officially interview anyone without a submission through HR. Please understand I will not talk to recruiters as we are not allowed to use them and I will not respond. I am sorry wish it were not the rule but it is and I can't change it. Best Regards, Pamela A Marcum AP Supervisor Histology Slot 502 4301 W Markham Street Little Rock AR 72205 Office: 501-686-7554 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From JMitchell <@t> uwhealth.org Fri Dec 2 09:53:32 2011 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Fri Dec 2 09:53:36 2011 Subject: [Histonet] EM Processors Message-ID: Looking for input from hospitals/institutions that process tissues for electron microscopy. I'm interested in what automated (such as Lynx) or microwave processors for EM are being utilized. What if any issues you have had and what you would recommended. Thanks for any and all feedback. Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 From Margaret.Perry <@t> sdstate.edu Fri Dec 2 11:04:14 2011 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Dec 2 11:04:20 2011 Subject: [Histonet] H&E stainers Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE01E90B@SDSU-EX03.jacks.local> We are in the market for a new H&E stainer. We probably won't be doing any special stains with it. Does anyone use the Shur/stain automated slide stainer by TBS? If so what kind of repair record does it have? Are you satisfied with it? Thanks for any info you would like to share. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From Clough <@t> medicine.tamhsc.edu Fri Dec 2 11:48:43 2011 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Fri Dec 2 11:49:05 2011 Subject: [Histonet] Microm STP120 and/or Linistain GLX-stainer Message-ID: I was wonder if anyone in the histonet community users a Microm STP-120 processor and /or a Linistain Random access GLX-stainer. If so, I would very much like to hear from you. Thanks, Bret Clough Texas A&M Health Science Center Temple,Texas From Stacy.Giroux <@t> stjohn.org Fri Dec 2 13:17:24 2011 From: Stacy.Giroux <@t> stjohn.org (Giroux, Stacy) Date: Fri Dec 2 13:17:51 2011 Subject: [Histonet] Xylene Recycler Message-ID: <29CCF3EC44815745864D9F84A1ED4B51B3DEF0E814@AUSP03VMBX11.apptixhealth.net> Our lab currently has a CBG BioTech xylene recycler. We only recycle xylene on the instrument not alcohol. Today while collecting our alcohol waste (which is usually clear) our techs noticed that the entire solution is cloudy; after sitting it appears to have some type of white material suspended within the entire solution with no separation layers. The recycled xylene purity was checked and it was normal. I am wondering if anyone has ever noticed this on their machines and if so if you have any insight into what this could possibly be caused from. About one month ago the computer and temperature probes were replaced on the instrument when after multiple attempts all xylene to be recycled was going into the alcohol waste container. Not sure if our new problem could be related to this previous issue. Thank you for your help. Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From ROrr <@t> northshore.org Fri Dec 2 13:20:19 2011 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Fri Dec 2 13:20:22 2011 Subject: [Histonet] Movat's Pentachrome in ChicagoLand? In-Reply-To: References: Message-ID: If anyone is running Movat's Pentachrome stain in the Chicago Area, could you please contact me? Thanks Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. From caithesketh <@t> gmail.com Fri Dec 2 13:25:37 2011 From: caithesketh <@t> gmail.com (Caitlin Hesketh) Date: Fri Dec 2 13:25:42 2011 Subject: [Histonet] Full time grossing tech wanted Message-ID: Fast growing laboratory in the Boston area is seeking a full-time grossing tech with experience. Shift 4-10pm M-F, somewhat flexible. Must be ASCP/HTL certified. Please contact jantonio@dnellc.com. From brett_connolly <@t> merck.com Fri Dec 2 13:30:31 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Dec 2 13:30:36 2011 Subject: [Histonet] Clostridium difficile - what do you do Message-ID: Histonetters- We are about to embark on a project involving C.diff infected GI tissue. We have processes in place for PPE, decontamination/ disinfection etc... but I am looking for any evidence whether or not formalin fixation or subsequent tissue processing will inactivate the spores. Also, do you take any special precautions in handling/sectioning the tissue /slides after paraffin embedding. I am not coming up with much info with my web searches, so I am asking for help from any lab that works with C. diff. Thanks, Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From KevinSeguin <@t> gmi-inc.com Fri Dec 2 13:39:54 2011 From: KevinSeguin <@t> gmi-inc.com (Kevin Seguin) Date: Fri Dec 2 13:40:00 2011 Subject: [Histonet] Using methyl benzoate on a VIP 1000 Message-ID: I have a customer who would like to use methyl benzoate on a VIP 1000. Sakura says that it is not on the list of reagents so it is not recommended. Can anyone shed some light on using methyl benzoate with a VIP 1000 or other tissue processors? Will this work? What are the problems? Thanks! Best regards, Kevin Seguin GMI www.gmi-inc.com kseguin@gmi-inc.com 800-745-2710 From Ronald.Houston <@t> nationwidechildrens.org Fri Dec 2 14:45:00 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Dec 2 14:45:09 2011 Subject: [Histonet] RE: Using methyl benzoate on a VIP 1000 In-Reply-To: References: Message-ID: not familiar with its use nowadays, but I remember it being combined with celloidin (methyl benzoate celloidin) as part of a double-embedding protocol used for autopsy tissues. Personally I have not heard of it being used alone. Can still remember "floating" in the room where we had all our open processors with chloroform, methyl benzoate, alcohols, xylene, celloidin and ether in the atmosphere. Oh happy days - we were still high when we got to the pub after work! Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kevin Seguin Sent: Friday, December 02, 2011 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Using methyl benzoate on a VIP 1000 I have a customer who would like to use methyl benzoate on a VIP 1000. Sakura says that it is not on the list of reagents so it is not recommended. Can anyone shed some light on using methyl benzoate with a VIP 1000 or other tissue processors? Will this work? What are the problems? Thanks! Best regards, Kevin Seguin GMI www.gmi-inc.com kseguin@gmi-inc.com 800-745-2710 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rjbuesa <@t> yahoo.com Fri Dec 2 14:52:51 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 2 14:52:54 2011 Subject: [Histonet] Using methyl benzoate on a VIP 1000 In-Reply-To: Message-ID: <1322859171.8096.YahooMailClassic@web65715.mail.ac4.yahoo.com> The problem could be that if something happens to the VIP Sakura will blame you and any guarantees will be?forfeit and repairs cost will increase, especially after you asked them. Ren? J. --- On Fri, 12/2/11, Kevin Seguin wrote: From: Kevin Seguin Subject: [Histonet] Using methyl benzoate on a VIP 1000 To: "histonet@lists.utsouthwestern.edu" Date: Friday, December 2, 2011, 2:39 PM I have a customer who would like to use methyl benzoate on a VIP 1000.? Sakura says that it is not on the list of reagents so it is not recommended.? Can anyone shed some light on using methyl benzoate with a VIP 1000 or other tissue processors?? Will this work?? What are the problems? Thanks! Best regards, Kevin Seguin GMI www.gmi-inc.com kseguin@gmi-inc.com 800-745-2710 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGleiberman <@t> cbiolabs.com Fri Dec 2 15:03:18 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Fri Dec 2 15:03:24 2011 Subject: [Histonet] RE: Using methyl benzoate on a VIP 1000 In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C4311CD46@cbiolabs05.CBiolabs.local> This thing was used as an intermediate step in paraffin processing of some difficult tissues such as whole mouse eye or amphibian eggs between xylene and paraffin. Awful smell, but some people like it - benzyl benzoate smells the same and is used as food preservative. Root beer has a strong smell of methyl (or benzyl) benzoate. Don't use it on VIP - your lab will smell during next several years. In my experience, use of cedar oil between xylene and paraffin (clearing in xylene, overnight incubation in cedar oil, short wash with benzene) works even better than methyl benzoate - and smells much better, for sure. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, December 02, 2011 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Using methyl benzoate on a VIP 1000 not familiar with its use nowadays, but I remember it being combined with celloidin (methyl benzoate celloidin) as part of a double-embedding protocol used for autopsy tissues. Personally I have not heard of it being used alone. Can still remember "floating" in the room where we had all our open processors with chloroform, methyl benzoate, alcohols, xylene, celloidin and ether in the atmosphere. Oh happy days - we were still high when we got to the pub after work! Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kevin Seguin Sent: Friday, December 02, 2011 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Using methyl benzoate on a VIP 1000 I have a customer who would like to use methyl benzoate on a VIP 1000. Sakura says that it is not on the list of reagents so it is not recommended. Can anyone shed some light on using methyl benzoate with a VIP 1000 or other tissue processors? Will this work? What are the problems? Thanks! Best regards, Kevin Seguin GMI www.gmi-inc.com kseguin@gmi-inc.com 800-745-2710 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From nto <@t> stowers.org Fri Dec 2 15:14:14 2011 From: nto <@t> stowers.org (Thomas, Nancy) Date: Fri Dec 2 15:14:20 2011 Subject: [Histonet] Chameleon tissue lifting off In-Reply-To: References: <2C40E43D1F7A56408C4463FD245DDDF993990281@EXCHMB-02.stowers-institute.org> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9939906CB@EXCHMB-02.stowers-institute.org> Scott, Thank you for your recommendation of the Superfrost ultra plus slides. I have just contacted the company for a sample box. I received a few other suggestions too, and will keep that info for possible future use. I will keep your email address, as well and maybe I will be able to help you sometime. Thanks to others who replied. Nancy Thomas From: Scott Parker [mailto:sparker@vt.edu] Sent: Friday, December 02, 2011 12:55 PM To: Thomas, Nancy Subject: Re: [Histonet] Chameleon tissue lifting off Hello Nancy, I work exclusively with squamate uterine and placental tissue so I know the frustration you are experiencing. Because placental tissues that I work with are so thin, separation is always a concern especially when using typical heat mediated antigen-retrieval techniques for immunohistochemistry. I actually have good success with Thermo Scientific Superfrost ultra plus slides. We have best success with 5 um sections and air drying slides slowly on a slide warmer. We then bake the slides for 30 minutes at 60 C. As usual, carefully sectioning is also the first step in achieving good tissue adherence. With delicate reptile placental tissues, any imperfections on the sectioning side will lead to greater tissue detachment. I hope this helps. We should stay in touch as there are not too many of us that work on reptile tissue in the histo-sphere. Scott Scott L. Parker, Ph.D. Assistant Professor of Biology Department of Biology Coastal Carolina University SC 29528-6054 On Wed, Nov 30, 2011 at 10:00 AM, Thomas, Nancy > wrote: I would like to ask anyone who sections chameleon tissue what type of slides they use. I was having too much lifting while using the superfrost plus slides. It looked like slides coated with Haupt's solution were highly recommended, so I tried that. It is so much better, but still there is some lifting. If someone is successful with sectioning and staining lizard tissue without lifting, please advise me on type of slides, drying times, or anything that might help. Thank you so much, Nancy Thomas Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From becksales <@t> optonline.net Fri Dec 2 15:22:01 2011 From: becksales <@t> optonline.net (becksales@optonline.net) Date: Fri Dec 2 15:22:04 2011 Subject: [Histonet] (no subject) Message-ID: <2074020698-1322860920-cardhu_decombobulator_blackberry.rim.net-513930361-@b2.c32.bise6.blackberry> Ok Sent via BlackBerry by AT&T From dw18 <@t> uchicago.edu Fri Dec 2 17:01:54 2011 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Fri Dec 2 17:01:58 2011 Subject: [Histonet] Re: Rbt on Rbt In-Reply-To: <201112021804.AQC34155@mx01.uchicago.edu> References: <201112021804.AQC34155@mx01.uchicago.edu> Message-ID: <20111202170154.BAR41141@mstore03.uchicago.edu> Hi Karen (& Histonet) Sorry for the long 'tutorial' here. In principle you can make your own rabbit on rabbit pre-treatment before adding your primary. The problem is specifically with any of the specimens's own IgG (rabbit, obviously) bound to your tissue. I haven't actually done it in rabbit but have dealt with the same problem in other rodent brains where any kind of damage (including the growth of tumors) makes the blood-brain barrier leaky so that the circulating IgG can seep out and bind to the brain tissue. You have to 'block' all this leaked IgG before you apply the anti-rabbit fluorescent secondary. Since you don't want to block your primary as well, it has to be done before the primary too. You block with a cold (unlabelled) anti-rabbit IgG antibody - basically a secondary without its label. How often are you going to do this? If you're not going to do this often enough to purchase the cold anti-rabbit, you could use any non-fluorescent anti-rabbit secondary. (In my freezer I have biotinylated goat anti-rabbit which would do for this.) Note that you have to cover up every last stray rabbit IgG, so you want to pre-incubate for much longer than your normal blocking step (with which it can be combined) - I would do it for as long as your normal primary incubation and/or at 2-5x your normal concentration for this non-fluorescent secondary. Don't forget to wash afterward, before the primary, or else you will neutralize your primary with the residue. Sorry this isn't a specific protocol, but I haven't seen any other reply (I only read the daily digest) -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 >Message: 1 >Date: Thu, 1 Dec 2011 10:01:31 -0800 >From: "Karen Cai" >Subject: [Histonet] Rabbit antibody on rabbit tissue Hi All, I am going to apply one rabbit polyclonal antibody on the rabbit tissue using IF. Is there anybody do it before? I heard of mouse on mouse IHC-DAB method, but not sure if it's possible in IF. Your kind response is very appreciated, Thanks, Karen From macveigh <@t> usc.edu Fri Dec 2 18:37:54 2011 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Fri Dec 2 18:37:59 2011 Subject: [Histonet] For H&E staining - TBS SHUR/Stain or Leica Autostainer XL Message-ID: <000601ccb153$cbcb4500$6361cf00$@usc.edu> I have and love my old Autostainer XL. Now we are offered a new TBS SHUR/Stain unit, which is attractively small, but I have never used nor seen it in person. We are a small University based lab and our space is limited. We rarely run few racks at a time. However, we must be able to run only deparaffination and only dehydration for our immunos. If any one has experience with the TBS unit, or even better, used both of them, please let me know what do you think. I would appreciate this very much Michelle From jkiernan <@t> uwo.ca Sat Dec 3 00:28:06 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Dec 3 00:28:11 2011 Subject: [Histonet] Using methyl benzoate on a VIP 1000 In-Reply-To: <75d0ce6c7d6a1.4ed9c14f@uwo.ca> References: <75c0bfb57894f.4ed9bde7@uwo.ca> <76a082077f39c.4ed9be24@uwo.ca> <76a0be597adb8.4ed9be61@uwo.ca> <7660d73b7fb10.4ed9be9f@uwo.ca> <76609439784b8.4ed9bedc@uwo.ca> <7660b6257ae1b.4ed9bf19@uwo.ca> <7630d63b7ea6f.4ed9bfcf@uwo.ca> <7580bac478ed2.4ed9c00c@uwo.ca> <7580a4ed7d4f1.4ed9c049@uwo.ca> <7660aae27ca89.4ed9c087@uwo.ca> <762098fc786a3.4ed9c096@uwo.ca> <75e094747b6b9.4ed9c0d4@uwo.ca> <7630ebdb799f0.4ed9c111@uwo.ca> <75d0ce6c7d6a1.4ed9c14f@uwo.ca> Message-ID: <74f086d57f262.4ed97b26@uwo.ca> Methyl benzoate smells really awful. Its only virtue is a high refractive index, which can make it useful for storing stained whole-mounts of small objects like chick embryos. Methyl salicylate (refractive index only slightly lower) is generally preferred because its wintergreen smell is quite pleasant. A recently introduced high refractive index fluid is 2,2'-thiodiethanol, which is miscible with water (Staudt et al 2007 Microscopy Research and Technique 70: 1-9). If you want a viscous and non-volatile clearing agent that remains in the wax and may help with cutting thin sections of very hard specimens, cedarwood oil is traditional, but terpineol (cheaper) is as good. I've used both, and agree with the recommendations in big old textbooks of microtechnique, especially Peter Gray (Microtomist's Formulary and Guide, 1954) and Manfred Gabe (Histological Techniques, 1976). John Kiernan Anatomy, UWO London, Canada = = = On 02/12/11, Kevin Seguin wrote: > > I have a customer who would like to use methyl benzoate on a VIP 1000. Sakura says that it is not on the list of reagents so it is not recommended. Can anyone shed some light on using methyl benzoate with a VIP 1000 or other tissue processors? Will this work? What are the problems? > > Thanks! > > > > Best regards, > > Kevin Seguin > GMI > www.gmi-inc.com > kseguin@gmi-inc.com> > 800-745-2710 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Sat Dec 3 08:46:24 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 3 08:46:28 2011 Subject: [Histonet] For H&E staining - TBS SHUR/Stain or Leica Autostainer XL In-Reply-To: <000601ccb153$cbcb4500$6361cf00$@usc.edu> Message-ID: <1322923584.86248.YahooMailClassic@web65712.mail.ac4.yahoo.com> You can dewax and hydrate your immunos much easier: use a 2% aq. vol:vol solution of dishwasher soap followed by tap and distilled water. As simple as that! The method has been tested and validated for immunos and is standard procedure of some labs.?If you?want I can provide you with?copies of these tests.?Ren? J. --- On Fri, 12/2/11, Michelle MacVeigh-Aloni wrote: From: Michelle MacVeigh-Aloni Subject: [Histonet] For H&E staining - TBS SHUR/Stain or Leica Autostainer XL To: histonet@lists.utsouthwestern.edu Date: Friday, December 2, 2011, 7:37 PM I have and love my old Autostainer XL. Now we are offered a new TBS SHUR/Stain unit, which is attractively small, but I have never used nor seen it in person. We are a small University based lab and our space is limited. We rarely run few racks at a time. However, we must be able to run only deparaffination and only dehydration for our immunos. If any one has experience with the TBS unit, or even better, used both of them, please let me know what do you think. I would appreciate this very much Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Dec 3 12:19:45 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Dec 3 12:19:50 2011 Subject: [Histonet] Re: Clostridium difficile - what do you do Message-ID: I have no idea - possibly no special precautions - I'd address this question - about Clostridium difficile - to the right person at the CDC. They'll know. While on the subject, how do you pronounce "difficile"? I say dih-FISS-illy - mostly on the basis of how Mozart pronounced it in his comic motet "Difficile lectu michi mars" (and never mind what that means in German). Bob Richmond Samurai Pathologist Knoxville TN ****************************** We are about to embark on a project involving C.diff infected GI tissue. We have processes in place for PPE, decontamination/ disinfection etc... but I am looking for any evidence whether or not formalin fixation or subsequent tissue processing will inactivate the spores. Also, do you take any special precautions in handling/sectioning the tissue /slides after paraffin embedding. I am not coming up with much info with my web searches, so I am asking for help from any lab that works with C. diff. Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 From trathborne <@t> somerset-healthcare.com Sat Dec 3 12:38:36 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sat Dec 3 12:38:52 2011 Subject: [Histonet] cassette marker Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F647A8@SMCMAIL01.somerset-healthcare.com> Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when placed in decalcifier. Vendors are welcome to respond. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From histotech <@t> imagesbyhopper.com Sat Dec 3 12:59:45 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Dec 3 13:00:00 2011 Subject: [Histonet] cassette marker In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F647A8@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711F647A8@SMCMAIL01.somerset-healthcare.com> Message-ID: <838178B6-ED4C-4CA8-B126-7FBCC4986CB5@imagesbyhopper.com> I like the StatLab markers. On Dec 3, 2011, at 1:38 PM, "Rathborne, Toni" wrote: Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when placed in decalcifier. Vendors are welcome to respond. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Dec 3 13:12:20 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Sat Dec 3 13:12:23 2011 Subject: [Histonet] cassette marker Message-ID: I like the securline marker II/superfrost. Used for years stay through reagents, AR, decal Sent from my Verizon Wireless BlackBerry -----Original Message----- From: histotech@imagesbyhopper.com Date: Sat, 3 Dec 2011 18:59:45 To: Cc: Subject: Re: [Histonet] cassette marker I like the StatLab markers. On Dec 3, 2011, at 1:38 PM, "Rathborne, Toni" wrote: Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when placed in decalcifier. Vendors are welcome to respond. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Dec 3 14:20:35 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 3 14:20:40 2011 Subject: [Histonet] As to "difficile" pronunciation. In-Reply-To: Message-ID: <1322943635.56313.YahooMailClassic@web65705.mail.ac4.yahoo.com> "Difficile" is italian for "difficult" and it is pronounced: difi-CHI-le" the "CHI" as in "cheese". Ren? J. --- On Sat, 12/3/11, Bob Richmond wrote: From: Bob Richmond Subject: [Histonet] Re: Clostridium difficile - what do you do To: histonet@lists.utsouthwestern.edu Date: Saturday, December 3, 2011, 1:19 PM I have no idea - possibly no special precautions - I'd address this question - about Clostridium difficile - to the right person at the CDC. They'll know. While on the subject, how do you pronounce "difficile"? I say dih-FISS-illy - mostly on the basis of how Mozart pronounced it in his comic motet "Difficile lectu michi mars" (and never mind what that means in German). Bob Richmond Samurai Pathologist Knoxville TN ****************************** We are about to embark on a project involving C.diff infected GI tissue. We have processes in place for PPE, decontamination/ disinfection etc... but I am looking for any evidence whether or not formalin fixation or subsequent tissue processing will inactivate the spores. Also, do you take any special precautions in handling/sectioning the tissue /slides after paraffin embedding. I am not coming up with much info with my web searches, so I am asking for help from any lab that works with C. diff. Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sat Dec 3 14:23:46 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Dec 3 14:23:50 2011 Subject: [Histonet] Re: Clostridium difficile - what do you do In-Reply-To: <76409bbdabccc.4eda852d@uwo.ca> References: <7610c346b29ed.4eda7c17@uwo.ca> <74a0c4d5b57a3.4eda7c53@uwo.ca> <7690975fb7fcd.4eda7c91@uwo.ca> <7490b463b37a2.4eda7cce@uwo.ca> <73c0fb3bb0180.4eda7d0c@uwo.ca> <74b0a132b5fef.4eda7d49@uwo.ca> <7630d7a8b0b38.4eda7d87@uwo.ca> <7630ae41b1465.4eda7dc5@uwo.ca> <7630e1b8b309e.4eda7dda@uwo.ca> <75a08717a8ec8.4eda83bb@uwo.ca> <7620a554af9d1.4eda83f9@uwo.ca> <7640e9b8aead7.4eda8436@uwo.ca> <7590ba6ca9e33.4eda8474@uwo.ca> <74e0ee2daa4bc.4eda84b1@uwo.ca> <75a0be45a8918.4eda84ef@uwo.ca> <76409bbdabccc.4eda852d@uwo.ca> Message-ID: <75a0bc02af79b.4eda3f02@uwo.ca> The school Latin pronunciation (classical rather than ecclesiastical) would be dif-ik-il-ay, but changing the k to s would be a reasonable anglicization, as is often done to make Latin names of plants and animals euphonious to anglophones. The name of this bacterium seems to be grammatically wrong because the nominative form is difficilis in all 3 cases. Difficile could mean "difficultly" but specific epithets are usually adjectives not adverbs. Newscasters nearly always say C. difficile with the last syllable rhyming with eel, as if it were a French word. This practice is is as ignorant as "the data is ...", or "what is the criteria for ...", or "a bacteria". Hrrrmph! Cheers, John Kiernan = = = On 03/12/11, Bob Richmond wrote: > > I have no idea - possibly no special precautions - I'd address this > question - about Clostridium difficile - to the right person at the > CDC. They'll know. > > While on the subject, how do you pronounce "difficile"? I say > dih-FISS-illy - mostly on the basis of how Mozart pronounced it in his > comic motet "Difficile lectu michi mars" (and never mind what that > means in German). > > Bob Richmond > Samurai Pathologist > Knoxville TN > ****************************** > We are about to embark on a project involving C.diff infected GI tissue. > > We have processes in place for PPE, decontamination/ disinfection > etc... but I am looking for any evidence whether or not formalin > fixation or subsequent tissue processing will inactivate the spores. > > Also, do you take any special precautions in handling/sectioning the > tissue /slides after paraffin embedding. > > I am not coming up with much info with my web searches, so I am asking > for help from any lab that works with C. diff. > > Brett M. Connolly, Ph.D. > Imaging Research Fellow > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rsrichmond <@t> gmail.com Sat Dec 3 15:15:56 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Dec 3 15:16:02 2011 Subject: [Histonet] Re: Clostridium difficile - what do you do In-Reply-To: <75a0bc02af79b.4eda3f02@uwo.ca> References: <7610c346b29ed.4eda7c17@uwo.ca> <74a0c4d5b57a3.4eda7c53@uwo.ca> <7690975fb7fcd.4eda7c91@uwo.ca> <7490b463b37a2.4eda7cce@uwo.ca> <73c0fb3bb0180.4eda7d0c@uwo.ca> <74b0a132b5fef.4eda7d49@uwo.ca> <7630d7a8b0b38.4eda7d87@uwo.ca> <7630ae41b1465.4eda7dc5@uwo.ca> <7630e1b8b309e.4eda7dda@uwo.ca> <75a08717a8ec8.4eda83bb@uwo.ca> <7620a554af9d1.4eda83f9@uwo.ca> <7640e9b8aead7.4eda8436@uwo.ca> <7590ba6ca9e33.4eda8474@uwo.ca> <74e0ee2daa4bc.4eda84b1@uwo.ca> <75a0be45a8918.4eda84ef@uwo.ca> <76409bbdabccc.4eda852d@uwo.ca> <75a0bc02af79b.4eda3f02@uwo.ca> Message-ID: It's "difficile" rather than *difficilis because it's a third declension singular neuter adjective, in apposition with "Clostridium", a second declension neuter. A reference to the Oxford-BBC Guide to Pronunciation suggests that my pronunciation is the correct one in English. Every European language has a "received pronunciation" of Latin, the English one being the weirdest, of course. The English one is never taught - you pick it up - I learned it from my mother. John Kiernan mentions a "school" or "classical" pronunciation - sometimes called "the Roman system" which you use only when you're reading classical Latin out loud when you're studying Latin. http://www.linguism.co.uk/language/the-oxford-bbc-guide-to-pronunciation "?di-fiss-i-li? [accent on "fiss" - there's a boldface in the original] is good anglicized Latin, and easy enough to say." The critical problem actually is where the accent on the word falls. As my final authority I offer none other than Wolfgang Amadeus Mozart, who wrote an exquisite motet on the German schoolboy joke "difficile lectu michi mars". If you listen to this YouTube http://www.youtube.com/watch?v=ps_JbyrN3zQ you'll hear four syllables, with the accent on the second syllable (you can read the music along with it in the YouTube). (The "c" is pronounced "ts" or "ch" - I'm not sure which - because they're using a German or Italian received pronunciation of Latin.) Here I think "difficile" is an adverb modifying the participial verb "lectu" (in reading). "Michi" (to me - spelt 'mihi' in classical Latin), "Mars" (Mars, for "military matters" is the subject. Now here's the schoolboy joke: "lectu michi Mars" sounds like German "leck Du mich im Arsch", for which a cognate English translation would be "lick thou me in [my] ass". Which I guess gets us back to the customary habitat of Clostridium difficile. Bob Richmond Samurai Pathologist (and occasional lexicographer, Dr. Johnson's harmless drudge) Knoxville TN ************************************************************ On Sat, Dec 3, 2011 at 3:23 PM, John Kiernan wrote: > The school Latin pronunciation (classical rather than ecclesiastical) would > be?dif-ik-il-ay, but changing the k to s would be a reasonable > anglicization, as is often done?to make?Latin names of plants and animals > euphonious to anglophones. The name of this bacterium seems to be > grammatically wrong because the nominative form is difficilis in all 3 > cases. Difficile could mean "difficultly" but specific epithets are usually > adjectives not adverbs. > > Newscasters nearly always say C. difficile with the last syllable rhyming > with eel, as if it were a French word. This practice is?is as ignorant as > "the data is ...", or "what is the criteria for ...", or "a bacteria". > Hrrrmph! > > Cheers,?? John Kiernan > = = = > On 03/12/11, Bob Richmond wrote: > > I have no idea - possibly no special precautions - I'd address this > question - about Clostridium difficile - to the right person at the > CDC. They'll know. > > While on the subject, how do you pronounce "difficile"? I say > dih-FISS-illy - mostly on the basis of how Mozart pronounced it in his > comic motet "Difficile lectu michi mars" (and never mind what that > means in German). > > Bob Richmond > Samurai Pathologist > Knoxville TN > ****************************** > We are about to embark on a project involving C.diff infected GI tissue. > > We have processes in place for PPE, decontamination/ disinfection > etc... but I am looking for any evidence whether or not formalin > fixation or subsequent tissue processing will inactivate the spores. > > Also, do you take any special precautions in handling/sectioning the > tissue /slides after paraffin embedding. > > I am not coming up with much info with my web searches, so I am asking > for help from any lab that works with C. diff. > > Brett M. Connolly, Ph.D. > Imaging Research Fellow > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 From rgeske_2000 <@t> yahoo.com Sat Dec 3 17:10:49 2011 From: rgeske_2000 <@t> yahoo.com (Rob Geske) Date: Sat Dec 3 17:10:52 2011 Subject: [Histonet] Clostridium difficile - what do you do Message-ID: <1322953849.4634.YahooMailClassic@web39402.mail.mud.yahoo.com> Hi Brett, ? you might want to take a look at the publication linked to below.? 2% glutaraldehyde is indicated in some of the (many) references from the publication and you might consider that option (spiking your NBF with 2% glut) if it will not interfere?with?any of the?procedures?you have planned for the tissues.? ? regards & bona fortuna, rob ? Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008 William A. Rutala, Ph.D., M.P.H.1,2, David J. Weber, M.D., M.P.H.1,2, and the Healthcare Infection Control Practices Advisory Committee (HICPAC)3 1Hospital Epidemiology University of North Carolina Health Care System Chapel Hill, NC 27514 2Division of Infectious Diseases University of North Carolina School of Medicine Chapel Hill, NC 27599-7030 1 ? ? http://www.cdc.gov/hicpac/pdf/guidelines/Disinfection_Nov_2008.pdf --- On Fri, 12/2/11, Connolly, Brett M wrote: From: Connolly, Brett M Subject: [Histonet] Clostridium difficile - what do you do To: "histonet@lists.utsouthwestern.edu" Date: Friday, December 2, 2011, 1:30 PM Histonetters- We are about to embark on a project involving C.diff infected GI tissue. We have processes in place for PPE, decontamination/ disinfection etc... but I am looking for any evidence whether or not formalin fixation or subsequent tissue processing will inactivate the spores. Also, do you take any special precautions in handling/sectioning the tissue /slides after paraffin embedding. I am not coming up with much info with my web searches, so I am asking for help from any lab that works with C. diff. Thanks, Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Dec 4 12:28:56 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Dec 4 12:29:02 2011 Subject: AW: [Histonet] Re: Clostridium difficile - what do you do In-Reply-To: References: Message-ID: <6E6CC4C0842740E783D6F4C99D894308@dielangs.at> Nom.: Clostridium difficile Gen.: Clostridii difficilis Dat.: Clostridio difficili Akk.: Clostridium difficile Voc.: Clostridio difficili I learned this pronounciation: 'klostri:dium di'fi:tsile in school. C is a "k" (like club) if a consonant follows and a "c" (like ts) if a vocal follows. But it's horrible, that after 5 years latin in school, I'm not able to form a sensefull sentence in this dead language. Clostridium difficile bacterium horribile est. Gudrun Lang Austria "Tu felix Austria nube!" = you lucky Austria marry; that was the better way to get more land. From rsrichmond <@t> gmail.com Sun Dec 4 14:31:19 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Dec 4 14:31:23 2011 Subject: [Histonet] Re: Clostridium difficile - what do you do In-Reply-To: <6E6CC4C0842740E783D6F4C99D894308@dielangs.at> References: <6E6CC4C0842740E783D6F4C99D894308@dielangs.at> Message-ID: di'fi:tsile is the German received pronunciation. I use it when I speak German, just as I use the English received pronunciation when I speak English. I didn't know "Tu felix Austria nube!" - When was it said, and in what historical setting? Hope you enjoyed the Mozart! Bob Richmond Samurai Pathologist Knoxville, Tennessee **************************************** On Sun, Dec 4, 2011 at 1:28 PM, Gudrun Lang wrote: > Nom.: Clostridium difficile > Gen.: Clostridii difficilis > Dat.: Clostridio difficili > Akk.: Clostridium difficile > Voc.: Clostridio difficili > > I learned this pronounciation: 'klostri:dium di'fi:tsile in school. C is a > "k" (like club) if a consonant follows and a "c" (like ts) if a vocal > follows. > > But it's horrible, that after 5 years latin in school, I'm not able to form > a sensefull sentence in this dead language. > Clostridium difficile bacterium horribile est. > > Gudrun Lang > Austria > "Tu felix Austria nube!" = you lucky Austria marry; that was the better way > to get more land. From tliglesias <@t> ucdavis.edu Sun Dec 4 16:55:44 2011 From: tliglesias <@t> ucdavis.edu (Teresa Iglesias) Date: Sun Dec 4 16:56:10 2011 Subject: [Histonet] nissl after permount? Message-ID: Hi all, I'm new to this so this may be a very dumb question but here goes. If you have already stained for IEGs (Zenk and cFos protein) in brain tissue and adhered coverslips with permount to the slides is it possible to then stain with nissl (after removing the covers, of course)? I'm having a hard time locating certain nuclei now (they were obvious before staining for IEGs and mounting) so I'm looking for a way to corroborate that I am looking in the right areas. As an alternative, I can stain new sections with nissl that have been kept in cryoprotectant and are free of IEG staining. IF I take this route, would I have to stain a replicate set of sections for ALL individuals (birds) so that I compare each IEG stained section to a nissl section that came from the same bird? In other words, I have 100 slides with 3+ sections per slide with IEG staining, will I need 100 slides with the same sections stained for nissl? Thanks for the help!! -Teresa -- ______________________________ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis From jkiernan <@t> uwo.ca Mon Dec 5 01:03:21 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Dec 5 01:03:25 2011 Subject: [Histonet] nissl after permount? In-Reply-To: <73c0bad4b5a2c.4edc6c83@uwo.ca> References: <75d0add1b7b7d.4edc649c@uwo.ca> <74b081f9b1c15.4edc64d9@uwo.ca> <7610d5cbb1d5d.4edc6516@uwo.ca> <73c0e303b0970.4edc6554@uwo.ca> <7690b9d0b5d9b.4edc6592@uwo.ca> <75d0badeb6a27.4edc65cf@uwo.ca> <75d0c72db3cca.4edc660d@uwo.ca> <7490a000b5f63.4edc664a@uwo.ca> <7630dffdb5acc.4edc6688@uwo.ca> <74a08053b320c.4edc67b5@uwo.ca> <7490ac97b4c56.4edc67f3@uwo.ca> <7610ac2fb211f.4edc6830@uwo.ca> <76108a73b02d5.4edc686e@uwo.ca> <74b0fa76b532a.4edc68ab@uwo.ca> <75d0a94db5cd3.4edc68e9@uwo.ca> <75d0aeffb18d2.4edc6926@uwo.ca> <74a0e535b3369.4edc6964@uwo.ca> <7530c9b9b522e.4edc69a1@uwo.ca> <76908269b3efd.4edc69de@uwo.ca> <7670a8eeb7dbb.4edc6a1c@uwo.ca> <73c0fea3b674f.4edc6a59@uwo.ca> <73c0866eb79e6.4edc6a97@uwo.ca> <73c0e317b6b21.4edc6ad4@uwo.ca> <76408380b0966.4edc6b12@uwo.ca> <7640e581b2c62.4edc6b4f@uwo.ca> <76709bbeb754e.4edc6b8d@uwo.ca> <7670d627b4404.4edc6bca@uwo.ca> <7670af7cb170a.4edc6c08@uwo.ca> <73c0bcd8b0893.4edc6c45@uwo.ca> <73c0bad4b5a2c.4edc6c83@uwo.ca> Message-ID: <73c0a67ab443a.4edc2669@uwo.ca> The usual approach would be to do Nissl stains on nearby (ideally adjacent) sections to those immunostained for the immediate early gene products. If you have no unstained slides (from bad planning), you will need to remove the coverslips from some of your slides, rehydrate and then do your Nissl stain. If the immunohistochemical product was oxidized DAB (brown), this will remain in place, and your sections will also have nuclear chromatin and nucleolar and cytoplasmic rRNA coloured blue, violet or red according to your choice of Nissl stain. Removing coverslips, extracting resinous mountant, rehydration and restaining is a tedious and time consuming job (days to a few weeks), especially if the slides are bearing thick sections (50-200um) or whole-mounts. As a graduate student, you should have an experienced faculty member to advise you. If your boss told you to ask on the Internet, he isn't earning his salary. There are many experienced histotechnologists at the University of California at Davis. Histotechs love to pass on their experience, learning and practical advice. You should still chase up your academic supervisor, who is paid from your "tuition" fees. John Kiernan Anatomy, UWO London, Canada = = = On 04/12/11, Teresa Iglesias wrote: > > Hi all, > I'm new to this so this may be a very dumb question but here goes. > If you have already stained for IEGs (Zenk and cFos protein) in brain > tissue and adhered coverslips with permount to the slides is it possible to > then stain with nissl (after removing the covers, of course)? > > I'm having a hard time locating certain nuclei now (they were obvious > before staining for IEGs and mounting) so I'm looking for a way to > corroborate that I am looking in the right areas. > > As an alternative, I can stain new sections with nissl that have been kept > in cryoprotectant and are free of IEG staining. IF I take this route, would > I have to stain a replicate set of sections for ALL individuals (birds) so > that I compare each IEG stained section to a nissl section that came from > the same bird? In other words, I have 100 slides with 3+ sections per slide > with IEG staining, will I need 100 slides with the same sections stained > for nissl? > > Thanks for the help!! > > -Teresa > > -- > ______________________________ > Teresa Iglesias > Graduate Group in Animal Behavior > Department of Evolution and Ecology > University of California-Davis > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From akbitting <@t> geisinger.edu Mon Dec 5 08:15:05 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Dec 5 08:15:33 2011 Subject: [Histonet] c-myc Message-ID: <4EDC8B98.2B7F.00C9.1@geisinger.edu> Does anyone send slides out for c-myc staining by IHC? Where do you send to? Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From Judith_Pardue <@t> memorial.org Mon Dec 5 08:24:17 2011 From: Judith_Pardue <@t> memorial.org (Pardue, Judith) Date: Mon Dec 5 08:24:26 2011 Subject: [Histonet] Tissue Loss Message-ID: <14B823F24E628E49BBFAD704E4BAB89A379B20@chimsx03.CHI.catholichealth.net> I would like some input on a situation I am faced with in our lab. We are constantly having tissue coming off the slide on GI bx's. This is coming from one tech, she is a senior tech and takes offense to questioning her about the problem. From what I can tell all of the techs are following the same protocal. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 judith_pardue@memorial.org Memorial Healthcare System To the world I'm one, to one I'm the world This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From rjbuesa <@t> yahoo.com Mon Dec 5 08:50:31 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 5 08:50:35 2011 Subject: [Histonet] Tissue Loss In-Reply-To: <14B823F24E628E49BBFAD704E4BAB89A379B20@chimsx03.CHI.catholichealth.net> Message-ID: <1323096631.21102.YahooMailClassic@web65706.mail.ac4.yahoo.com> First of all disregard any "offense" any tech may take when? you have something to say about the work result. The patient's care overrules "any offense". Tissues can come off, specially in GI biopsies if they dry out too much during processing with a general protocol where dehydration results too much for them. Also cutting too fast and not letting the sections to drain correctly before drying in the oven to be stained, can cause them to fall off. Check?how your "senior tech" cuts, and if it is too fast, tell?her to slow down a bit. Compare how she works and how the other techs work and?if you see any technical difference, that is probably the cause and "offended" or not tell her to follow standard procedure. A tech should care for the quality of the work and in the histology laboratory there is no place for "histo-divas". Ren? J. --- On Mon, 12/5/11, Pardue, Judith wrote: From: Pardue, Judith Subject: [Histonet] Tissue Loss To: histonet@lists.utsouthwestern.edu Date: Monday, December 5, 2011, 9:24 AM I would like some input on a situation I am faced with in our lab. We are constantly having tissue coming off the slide on GI bx's. This is coming from one tech, she is a senior tech and takes offense to questioning her about the problem. From what I can tell all of the techs are following the same protocal. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 judith_pardue@memorial.org Memorial Healthcare System To the world I'm one, to one I'm the world This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information.? If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Mon Dec 5 09:21:43 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Dec 5 09:21:51 2011 Subject: [Histonet] RE: cassette marker In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F647A8@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711F647A8@SMCMAIL01.somerset-healthcare.com> Message-ID: Laboratory Marking Pens from Thermo Scientific (Richard-Allan) Ref 2000. Best for staying on but sensitive to writing on surfaces that are not absolutely dry. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Saturday, December 03, 2011 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette marker Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when placed in decalcifier. Vendors are welcome to respond. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Mon Dec 5 09:44:50 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Mon Dec 5 09:45:56 2011 Subject: [Histonet] RE: cassette marker In-Reply-To: References: <3AD061FE740D464FAC7BF6B5CFB7570711F647A8@SMCMAIL01.somerset-healthcare.com> Message-ID: <4901.208.62.167.196.1323099890.squirrel@webmail.realpages.com> I absolutely love mercedes medical pens called platunium line mer marker. They are smudge prrof and stay on. Love them. Im sure if you called CHAD at mercedes he could send you a free sample. Nicole Tatum Laboratory Marking Pens from Thermo Scientific (Richard-Allan) Ref 2000. > Best for staying on but sensitive to writing on surfaces that are not > absolutely dry. > > > > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, > Toni > Sent: Saturday, December 03, 2011 11:39 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cassette marker > > > > Can anyone recommend a marker for using on cassettes? We currently use > pencil, which sometimes smudges. We've tried a few markers already, but > some fade, while others hold up well for processing, but won't when placed > in decalcifier. > > Vendors are welcome to respond. > > Thanks, > > Toni > > > > > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical Center > > and are intended only for the addressee. The information contained in > this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection and/or > > exemption from disclosure under applicable law. Unauthorized forwarding, > > printing, copying, distribution, or use of such information is strictly > > prohibited and may be unlawful. If you are not the addressee, please > > promptly delete this message and notify the sender of the delivery error > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, > > event listings, health information and more. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From POWELL_SA <@t> mercer.edu Mon Dec 5 10:17:27 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Dec 5 10:17:34 2011 Subject: [Histonet] RE: Tissue Loss In-Reply-To: <14B823F24E628E49BBFAD704E4BAB89A379B20@chimsx03.CHI.catholichealth.net> References: <14B823F24E628E49BBFAD704E4BAB89A379B20@chimsx03.CHI.catholichealth.net> Message-ID: <9BF995BC0E47744E9673A41486E24EE24AA1325B9B@MERCERMAIL.MercerU.local> If she is using hand lotion and handles the slides, that will make the come off. She needs to take all the lotion off before cutting slides. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Monday, December 05, 2011 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Loss I would like some input on a situation I am faced with in our lab. We are constantly having tissue coming off the slide on GI bx's. This is coming from one tech, she is a senior tech and takes offense to questioning her about the problem. From what I can tell all of the techs are following the same protocal. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 judith_pardue@memorial.org Memorial Healthcare System To the world I'm one, to one I'm the world This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Mon Dec 5 10:27:01 2011 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Mon Dec 5 10:27:08 2011 Subject: [Histonet] Cassettes not fitting in molds Message-ID: <1323102421.70461.YahooMailNeo@web161603.mail.bf1.yahoo.com> Hi all, I just purchased new metal molds from a new vendor and my cassettes aren't fitting flat into them. Either the front or back is lifted. I use tissue tek uni-cassettes. I will say the molds I purchased weren't Sakura. Any suggestions? Maybe different cassette? Thanks in advance, Jill Jill Cox, HT ASCP From Rcartun <@t> harthosp.org Mon Dec 5 11:17:59 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Dec 5 11:18:28 2011 Subject: [Histonet] Question - CPT Coding 88321 Message-ID: <4EDCB677.7400.0077.1@harthosp.org> I'm confused (what's new!). I'm being told there is no "technical component" (TC) for 88321 (slide consultation). Is that correct? There has to be a technical component to cover accessioning. Thanks for your help. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Albert.Santiago <@t> uphs.upenn.edu Mon Dec 5 12:10:51 2011 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Mon Dec 5 12:10:58 2011 Subject: [Histonet] RE: Histonet Digest, Vol 97, Issue 6 In-Reply-To: References: Message-ID: Hi Toni, we've had that problem also. The best solution we've been able to find out is a pen called KP Marker Plus. You can find it at www.klinipath.com. I hope that helps, good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, December 05, 2011 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 97, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. AW: [Histonet] Re: Clostridium difficile - what do you do (Gudrun Lang) 2. Re: Re: Clostridium difficile - what do you do (Bob Richmond) 3. nissl after permount? (Teresa Iglesias) 4. Re: nissl after permount? (John Kiernan) 5. c-myc (Angela Bitting) 6. Tissue Loss (Pardue, Judith) 7. Re: Tissue Loss (Rene J Buesa) 8. RE: cassette marker (Goins, Tresa) 9. Re: RE: cassette marker (Nicole Tatum) 10. RE: Tissue Loss (Shirley A. Powell) 11. Cassettes not fitting in molds (Jill Cox) 12. Question - CPT Coding 88321 (Richard Cartun) ---------------------------------------------------------------------- Message: 1 Date: Sun, 4 Dec 2011 19:28:56 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Re: Clostridium difficile - what do you do To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <6E6CC4C0842740E783D6F4C99D894308@dielangs.at> Content-Type: text/plain; charset="us-ascii" Nom.: Clostridium difficile Gen.: Clostridii difficilis Dat.: Clostridio difficili Akk.: Clostridium difficile Voc.: Clostridio difficili I learned this pronounciation: 'klostri:dium di'fi:tsile in school. C is a "k" (like club) if a consonant follows and a "c" (like ts) if a vocal follows. But it's horrible, that after 5 years latin in school, I'm not able to form a sensefull sentence in this dead language. Clostridium difficile bacterium horribile est. Gudrun Lang Austria "Tu felix Austria nube!" = you lucky Austria marry; that was the better way to get more land. ------------------------------ Message: 2 Date: Sun, 4 Dec 2011 15:31:19 -0500 From: Bob Richmond Subject: Re: [Histonet] Re: Clostridium difficile - what do you do To: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 di'fi:tsile is the German received pronunciation. I use it when I speak German, just as I use the English received pronunciation when I speak English. I didn't know "Tu felix Austria nube!" - When was it said, and in what historical setting? Hope you enjoyed the Mozart! Bob Richmond Samurai Pathologist Knoxville, Tennessee **************************************** On Sun, Dec 4, 2011 at 1:28 PM, Gudrun Lang wrote: > Nom.: Clostridium difficile > Gen.: Clostridii difficilis > Dat.: Clostridio difficili > Akk.: Clostridium difficile > Voc.: Clostridio difficili > > I learned this pronounciation: 'klostri:dium di'fi:tsile in school. C is a > "k" (like club) if a consonant follows and a "c" (like ts) if a vocal > follows. > > But it's horrible, that after 5 years latin in school, I'm not able to form > a sensefull sentence in this dead language. > Clostridium difficile bacterium horribile est. > > Gudrun Lang > Austria > "Tu felix Austria nube!" = you lucky Austria marry; that was the better way > to get more land. ------------------------------ Message: 3 Date: Sun, 4 Dec 2011 16:55:44 -0600 From: Teresa Iglesias Subject: [Histonet] nissl after permount? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi all, I'm new to this so this may be a very dumb question but here goes. If you have already stained for IEGs (Zenk and cFos protein) in brain tissue and adhered coverslips with permount to the slides is it possible to then stain with nissl (after removing the covers, of course)? I'm having a hard time locating certain nuclei now (they were obvious before staining for IEGs and mounting) so I'm looking for a way to corroborate that I am looking in the right areas. As an alternative, I can stain new sections with nissl that have been kept in cryoprotectant and are free of IEG staining. IF I take this route, would I have to stain a replicate set of sections for ALL individuals (birds) so that I compare each IEG stained section to a nissl section that came from the same bird? In other words, I have 100 slides with 3+ sections per slide with IEG staining, will I need 100 slides with the same sections stained for nissl? Thanks for the help!! -Teresa -- ______________________________ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis ------------------------------ Message: 4 Date: Mon, 05 Dec 2011 02:03:21 -0500 From: John Kiernan Subject: Re: [Histonet] nissl after permount? To: Teresa Iglesias , histonet@lists.utsouthwestern.edu Message-ID: <73c0a67ab443a.4edc2669@uwo.ca> Content-Type: text/plain; CHARSET=US-ASCII The usual approach would be to do Nissl stains on nearby (ideally adjacent) sections to those immunostained for the immediate early gene products. If you have no unstained slides (from bad planning), you will need to remove the coverslips from some of your slides, rehydrate and then do your Nissl stain. If the immunohistochemical product was oxidized DAB (brown), this will remain in place, and your sections will also have nuclear chromatin and nucleolar and cytoplasmic rRNA coloured blue, violet or red according to your choice of Nissl stain. Removing coverslips, extracting resinous mountant, rehydration and restaining is a tedious and time consuming job (days to a few weeks), especially if the slides are bearing thick sections (50-200um) or whole-mounts. As a graduate student, you should have an experienced faculty member to advise you. If your boss told you to ask on the Internet, he isn't earning his salary. There are many experienced histotechnologists at the University of California at Davis. Histotechs love to pass on their experience, learning and practical advice. You should still chase up your academic supervisor, who is paid from your "tuition" fees. John Kiernan Anatomy, UWO London, Canada = = = On 04/12/11, Teresa Iglesias wrote: > > Hi all, > I'm new to this so this may be a very dumb question but here goes. > If you have already stained for IEGs (Zenk and cFos protein) in brain > tissue and adhered coverslips with permount to the slides is it possible to > then stain with nissl (after removing the covers, of course)? > > I'm having a hard time locating certain nuclei now (they were obvious > before staining for IEGs and mounting) so I'm looking for a way to > corroborate that I am looking in the right areas. > > As an alternative, I can stain new sections with nissl that have been kept > in cryoprotectant and are free of IEG staining. IF I take this route, would > I have to stain a replicate set of sections for ALL individuals (birds) so > that I compare each IEG stained section to a nissl section that came from > the same bird? In other words, I have 100 slides with 3+ sections per slide > with IEG staining, will I need 100 slides with the same sections stained > for nissl? > > Thanks for the help!! > > -Teresa > > -- > ______________________________ > Teresa Iglesias > Graduate Group in Animal Behavior > Department of Evolution and Ecology > University of California-Davis > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 5 Date: Mon, 05 Dec 2011 09:15:05 -0500 From: "Angela Bitting" Subject: [Histonet] c-myc To: Message-ID: <4EDC8B98.2B7F.00C9.1@geisinger.edu> Content-Type: text/plain; charset="us-ascii" Does anyone send slides out for c-myc staining by IHC? Where do you send to? Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 6 Date: Mon, 5 Dec 2011 07:24:17 -0700 From: "Pardue, Judith" Subject: [Histonet] Tissue Loss To: Message-ID: <14B823F24E628E49BBFAD704E4BAB89A379B20@chimsx03.CHI.catholichealth.net> Content-Type: text/plain; charset="ISO-8859-1" I would like some input on a situation I am faced with in our lab. We are constantly having tissue coming off the slide on GI bx's. This is coming from one tech, she is a senior tech and takes offense to questioning her about the problem. From what I can tell all of the techs are following the same protocal. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 judith_pardue@memorial.org Memorial Healthcare System To the world I'm one, to one I'm the world This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 7 Date: Mon, 5 Dec 2011 06:50:31 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Tissue Loss To: histonet@lists.utsouthwestern.edu, JudithPardue Message-ID: <1323096631.21102.YahooMailClassic@web65706.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 First of all disregard any "offense" any tech may take when? you have something to say about the work result. The patient's care overrules "any offense". Tissues can come off, specially in GI biopsies if they dry out too much during processing with a general protocol where dehydration results too much for them. Also cutting too fast and not letting the sections to drain correctly before drying in the oven to be stained, can cause them to fall off. Check?how your "senior tech" cuts, and if it is too fast, tell?her to slow down a bit. Compare how she works and how the other techs work and?if you see any technical difference, that is probably the cause and "offended" or not tell her to follow standard procedure. A tech should care for the quality of the work and in the histology laboratory there is no place for "histo-divas". Ren? J. --- On Mon, 12/5/11, Pardue, Judith wrote: From: Pardue, Judith Subject: [Histonet] Tissue Loss To: histonet@lists.utsouthwestern.edu Date: Monday, December 5, 2011, 9:24 AM I would like some input on a situation I am faced with in our lab. We are constantly having tissue coming off the slide on GI bx's. This is coming from one tech, she is a senior tech and takes offense to questioning her about the problem. From what I can tell all of the techs are following the same protocal. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 judith_pardue@memorial.org Memorial Healthcare System To the world I'm one, to one I'm the world This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information.? If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 5 Dec 2011 15:21:43 +0000 From: "Goins, Tresa" Subject: [Histonet] RE: cassette marker To: "Rathborne, Toni" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Laboratory Marking Pens from Thermo Scientific (Richard-Allan) Ref 2000. Best for staying on but sensitive to writing on surfaces that are not absolutely dry. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Saturday, December 03, 2011 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette marker Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when placed in decalcifier. Vendors are welcome to respond. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 5 Dec 2011 10:44:50 -0500 (EST) From: "Nicole Tatum" Subject: Re: [Histonet] RE: cassette marker To: "Goins, Tresa" , histonet@lists.utsouthwestern.edu Message-ID: <4901.208.62.167.196.1323099890.squirrel@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 I absolutely love mercedes medical pens called platunium line mer marker. They are smudge prrof and stay on. Love them. Im sure if you called CHAD at mercedes he could send you a free sample. Nicole Tatum Laboratory Marking Pens from Thermo Scientific (Richard-Allan) Ref 2000. > Best for staying on but sensitive to writing on surfaces that are not > absolutely dry. > > > > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, > Toni > Sent: Saturday, December 03, 2011 11:39 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cassette marker > > > > Can anyone recommend a marker for using on cassettes? We currently use > pencil, which sometimes smudges. We've tried a few markers already, but > some fade, while others hold up well for processing, but won't when placed > in decalcifier. > > Vendors are welcome to respond. > > Thanks, > > Toni > > > > > > > > > > CONFIDENTIALITY NOTICE > > This message and any included attachments are from Somerset Medical Center > > and are intended only for the addressee. The information contained in > this > > message is confidential and may contain privileged, confidential, > > proprietary and/or trade secret information entitled to protection and/or > > exemption from disclosure under applicable law. Unauthorized forwarding, > > printing, copying, distribution, or use of such information is strictly > > prohibited and may be unlawful. If you are not the addressee, please > > promptly delete this message and notify the sender of the delivery error > > by e-mail or you may call Somerset Medical Center's computer Help Desk > > at 908-685-2200, ext. 4050. > > > > Be sure to visit Somerset Medical Center's Web site - > > www.somersetmedicalcenter.com - for the most up-to-date news, > > event listings, health information and more. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Mon, 5 Dec 2011 11:17:27 -0500 From: "Shirley A. Powell" Subject: [Histonet] RE: Tissue Loss To: "Pardue, Judith" , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE24AA1325B9B@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" If she is using hand lotion and handles the slides, that will make the come off. She needs to take all the lotion off before cutting slides. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Monday, December 05, 2011 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Loss I would like some input on a situation I am faced with in our lab. We are constantly having tissue coming off the slide on GI bx's. This is coming from one tech, she is a senior tech and takes offense to questioning her about the problem. From what I can tell all of the techs are following the same protocal. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 judith_pardue@memorial.org Memorial Healthcare System To the world I'm one, to one I'm the world This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 5 Dec 2011 08:27:01 -0800 (PST) From: Jill Cox Subject: [Histonet] Cassettes not fitting in molds To: "Histonet@Lists. Edu" Message-ID: <1323102421.70461.YahooMailNeo@web161603.mail.bf1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi all, I just purchased new metal molds from a new vendor and my cassettes aren't fitting flat into them. Either the front or back is lifted. I use tissue tek uni-cassettes. I will say the molds I purchased weren't Sakura. Any suggestions? Maybe different cassette? Thanks in advance, Jill Jill Cox, HT ASCP ------------------------------ Message: 12 Date: Mon, 05 Dec 2011 12:17:59 -0500 From: "Richard Cartun" Subject: [Histonet] Question - CPT Coding 88321 To: "Histonet" Message-ID: <4EDCB677.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII I'm confused (what's new!). I'm being told there is no "technical component" (TC) for 88321 (slide consultation). Is that correct? There has to be a technical component to cover accessioning. Thanks for your help. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 97, Issue 6 *************************************** The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From JWeems <@t> sjha.org Mon Dec 5 12:37:07 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Dec 5 12:37:49 2011 Subject: [Histonet] Question - CPT Coding 88321 In-Reply-To: <4EDCB677.7400.0077.1@harthosp.org> References: <4EDCB677.7400.0077.1@harthosp.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408454D1313@CHEXCMS10.one.ads.che.org> Sorry. There is not. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Monday, December 05, 2011 12:17 PM To: Histonet Subject: [Histonet] Question - CPT Coding 88321 I'm confused (what's new!). I'm being told there is no "technical component" (TC) for 88321 (slide consultation). Is that correct? There has to be a technical component to cover accessioning. Thanks for your help. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tliglesias <@t> ucdavis.edu Mon Dec 5 13:05:52 2011 From: tliglesias <@t> ucdavis.edu (Teresa Iglesias) Date: Mon Dec 5 13:06:19 2011 Subject: [Histonet] nissl after permount? In-Reply-To: <73c0a67ab443a.4edc2669@uwo.ca> References: <75d0add1b7b7d.4edc649c@uwo.ca> <74b081f9b1c15.4edc64d9@uwo.ca> <7610d5cbb1d5d.4edc6516@uwo.ca> <73c0e303b0970.4edc6554@uwo.ca> <7690b9d0b5d9b.4edc6592@uwo.ca> <75d0badeb6a27.4edc65cf@uwo.ca> <75d0c72db3cca.4edc660d@uwo.ca> <7490a000b5f63.4edc664a@uwo.ca> <7630dffdb5acc.4edc6688@uwo.ca> <74a08053b320c.4edc67b5@uwo.ca> <7490ac97b4c56.4edc67f3@uwo.ca> <7610ac2fb211f.4edc6830@uwo.ca> <76108a73b02d5.4edc686e@uwo.ca> <74b0fa76b532a.4edc68ab@uwo.ca> <75d0a94db5cd3.4edc68e9@uwo.ca> <75d0aeffb18d2.4edc6926@uwo.ca> <74a0e535b3369.4edc6964@uwo.ca> <7530c9b9b522e.4edc69a1@uwo.ca> <76908269b3efd.4edc69de@uwo.ca> <7670a8eeb7dbb.4edc6a1c@uwo.ca> <73c0fea3b674f.4edc6a59@uwo.ca> <73c0866eb79e6.4edc6a97@uwo.ca> <73c0e317b6b21.4edc6ad4@uwo.ca> <76408380b0966.4edc6b12@uwo.ca> <7640e581b2c62.4edc6b4f@uwo.ca> <76709bbeb754e.4edc6b8d@uwo.ca> <7670d627b4404.4edc6bca@uwo.ca> <7670af7cb170a.4edc6c08@uwo.ca> <73c0bcd8b0893.4edc6c45@uwo.ca> <73c0bad4b5a2c.4edc6c83@uwo.ca> <73c0a67ab443a.4edc2669@uwo.ca> Message-ID: Thank you for the excellent information. -Teresa On Mon, Dec 5, 2011 at 1:03 AM, John Kiernan wrote: > The usual approach would be to do Nissl stains on nearby (ideally > adjacent) sections to those immunostained for the immediate early gene > products. If you have no unstained slides (from bad planning), you will > need to remove the coverslips from some of your slides, rehydrate and then > do your Nissl stain. If the immunohistochemical product was oxidized DAB > (brown), this will remain in place, and your sections will also have > nuclear chromatin and nucleolar and cytoplasmic rRNA coloured blue, violet > or red according to your choice of Nissl stain. > > Removing coverslips, extracting resinous mountant, rehydration and > restaining is a tedious and time consuming job (days to a few > weeks), especially if the slides are bearing thick sections (50-200um) or > whole-mounts. > > As a graduate student, you should have an experienced faculty member to > advise you. If your boss told you to ask on the Internet, he isn't earning > his salary. > There are many experienced histotechnologists at the University of > California at Davis. Histotechs love to pass on their experience, learning > and practical advice. You should still chase up your academic supervisor, > who is paid from your "tuition" fees. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > On 04/12/11, *Teresa Iglesias * wrote: > > Hi all, > I'm new to this so this may be a very dumb question but here goes. > If you have already stained for IEGs (Zenk and cFos protein) in brain > tissue and adhered coverslips with permount to the slides is it possible to > then stain with nissl (after removing the covers, of course)? > > I'm having a hard time locating certain nuclei now (they were obvious > before staining for IEGs and mounting) so I'm looking for a way to > corroborate that I am looking in the right areas. > > As an alternative, I can stain new sections with nissl that have been kept > in cryoprotectant and are free of IEG staining. IF I take this route, would > I have to stain a replicate set of sections for ALL individuals (birds) so > that I compare each IEG stained section to a nissl section that came from > the same bird? In other words, I have 100 slides with 3+ sections per slide > with IEG staining, will I need 100 slides with the same sections stained > for nissl? > > Thanks for the help!! > > -Teresa > > -- > ______________________________ > Teresa Iglesias > Graduate Group in Animal Behavior > Department of Evolution and Ecology > University of California-Davis > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- ______________________________ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis One Shields Avenue 2320 Storer Hall Davis, CA 95616 Office: 530-754-7837 Fax: 530-752-1449 tliglesias@ucdavis.edu ______________________________ From PMonfils <@t> Lifespan.org Mon Dec 5 14:01:06 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Dec 5 14:01:20 2011 Subject: [Histonet] Shandon Tissue Specimen Bags Message-ID: <4EBFF65383B74D49995298C4976D1D5E08FCA3D7@LSRIEXCH1.lsmaster.lifespan.org> Shandon Tissue Specimen Bags (nylon biopsy bags) - are they available anywhere? Information on other brands also welcome, but I have used the Shandon bags for many years and am very satisfied with them. Thanks. From rjbuesa <@t> yahoo.com Mon Dec 5 14:22:47 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 5 14:22:51 2011 Subject: [Histonet] Shandon Tissue Specimen Bags In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E08FCA3D7@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <1323116567.32431.YahooMailClassic@web65710.mail.ac4.yahoo.com> Use empty tea bags. They are as good as the nylon ones. Ren? J. --- On Mon, 12/5/11, Monfils, Paul wrote: From: Monfils, Paul Subject: [Histonet] Shandon Tissue Specimen Bags To: histonet@lists.utsouthwestern.edu Date: Monday, December 5, 2011, 3:01 PM Shandon Tissue Specimen Bags (nylon biopsy bags) - are they available anywhere?? Information on other brands also welcome, but I have used the Shandon bags for many years and am very satisfied with them. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renetimlee <@t> aol.com Mon Dec 5 19:26:39 2011 From: renetimlee <@t> aol.com (Tim) Date: Mon Dec 5 19:26:49 2011 Subject: [Histonet] thermo excelsior es Message-ID: <8CE81CD3C58B0DA-C7C-E49C@webmail-d133.sysops.aol.com> what are the disadvantages and advantages of excelsior ES tissue processor? From Susan.Walzer <@t> HCAHealthcare.com Tue Dec 6 02:07:26 2011 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Dec 6 02:07:31 2011 Subject: [Histonet] RE: Tissue Loss In-Reply-To: <14B823F24E628E49BBFAD704E4BAB89A379B20@chimsx03.CHI.catholichealth.net> References: <14B823F24E628E49BBFAD704E4BAB89A379B20@chimsx03.CHI.catholichealth.net> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DDD7E5A44@FWDCWPMSGCMS09.hca.corpad.net> When tissue falls off the slide it is usually poorly cut sections or a problem with under processing or bad tissue ( bones) GI biopsies should not be falling off if cut well and processed correctly. Keep an eye on how she is cutting, she may have a problem with her microtome ( are all screws tight and is the angle right?) Is she cutting too thick? Is she ribboning? If a tech cannot get a ribbon on a biopsy she is doing something wrong. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Monday, December 05, 2011 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Loss I would like some input on a situation I am faced with in our lab. We are constantly having tissue coming off the slide on GI bx's. This is coming from one tech, she is a senior tech and takes offense to questioning her about the problem. From what I can tell all of the techs are following the same protocal. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 judith_pardue@memorial.org Memorial Healthcare System To the world I'm one, to one I'm the world This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From we3smitty <@t> yahoo.com Tue Dec 6 07:20:03 2011 From: we3smitty <@t> yahoo.com (angela smith) Date: Tue Dec 6 07:20:09 2011 Subject: [Histonet] TNF-alpha Message-ID: <1323177603.3752.YahooMailClassic@web125403.mail.ne1.yahoo.com> Does From we3smitty <@t> yahoo.com Tue Dec 6 07:20:04 2011 From: we3smitty <@t> yahoo.com (angela smith) Date: Tue Dec 6 07:20:11 2011 Subject: [Histonet] TNF-alpha Message-ID: <1323177604.95920.YahooMailClassic@web125403.mail.ne1.yahoo.com> Does anyone From we3smitty <@t> yahoo.com Tue Dec 6 07:20:04 2011 From: we3smitty <@t> yahoo.com (angela smith) Date: Tue Dec 6 07:20:13 2011 Subject: [Histonet] TNF-alpha Message-ID: <1323177604.89293.YahooMailClassic@web125403.mail.ne1.yahoo.com> Does anyone have From we3smitty <@t> yahoo.com Tue Dec 6 07:21:34 2011 From: we3smitty <@t> yahoo.com (angela smith) Date: Tue Dec 6 07:21:36 2011 Subject: [Histonet] TNF alpha Message-ID: <1323177694.52645.YahooMailClassic@web125406.mail.ne1.yahoo.com> Does anyone have successful experience with TNF-alpha on Mouse tissue?? If so where did you get the antibody, what is your procedure and did you use automation?? Please share if you will. Thank you, Angela Smith Histology Supervisor Children's Mercy Hospital From SteveM <@t> mcclainlab.com Tue Dec 6 07:57:05 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Tue Dec 6 07:57:01 2011 Subject: [Histonet] RE: Histonet Digest, Vol 97, Issue 5 Cassette Marking Slide labeling Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF16E34D@ML1.McClainLabs.local> Leica sells the Surgipath brand cat. #3801880 a decent felt-type solvent resistant marker my techs like. (still have and like hand written slides and techs initials). Those black Tissue-Tek histo pencils are good too if you write tall, but for slides can carry some graphite dust onto the tissue sections.. High Quality Colored pencils stay on cassettes surprisingly well though alc-xyl, as our Jamaican friend Dr. Jennifer Alexander and taught us this. Looking to buy used RA Lamb Microwriter may not be ethical in this forum, but expresses my admiration for those slow, but dependable workhorses. We turn ours on and off maybe 4 times a year. They'll even print 2D barcodes if you give them 25 seconds, but the BC do not read readily when covered by paraffin and we eventually removed the BC from cassettes. Those Microwriters work for years on little maintenance- every 100,000-200,000 cassettes. Permanent in Decal Acid KOH Xylene. However, even those cassette labels can smudge if you heat and wipe the label area, e.g., when melting paraffin from the block edges prior to trimming in and sectioning. Anyone know of a good used Microwriter available, please let me know. The slide/cassette-writers using UV curing ink worked well in our tests- barcode read well. However, they depend on invisible UV light to work and failure to change bulbs could potentially lead to rookie-type disastrous non-labeling or washing off labels from cassettes. Changing cassette brands/ bad ink/ bad bulbs may each cause a failure. Telling 100-200 patients their specimens were effectively lost in processing because the cassette label dissolved away is not on my bucket list. I almost missed bullous pemphigoid on a direct immunofluorescence case once because of premature UV bulb failure at ~80 hours- this doctor failed to change the bulb in the UV scope. It seems possible the same thing might happen with the flash bulbs type ink jet label curing. I repeated the DIF (not surprisingly with same result) And then took the slides to a different scope, and now I change UV microscope bulbs at 120-180 hours . An LED UV source should be stable for years. Several years ago we looked at laser etchers for blocks and slides and thought they had potential, yet seemed pricey for consumables. Technical labeling and barcoding is a subject for another time. Steve 631 361 4000 ***************** From Clough <@t> medicine.tamhsc.edu Tue Dec 6 10:19:05 2011 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Tue Dec 6 10:20:28 2011 Subject: [Histonet] Looking for a very good refurbished rotary microtome to purchase. Message-ID: I am trying to locate a really good inexpensive rotary microtome to purchase for our research lab. Any thoughts as to where to look and what model to get? We currently have a Lieca 1512 and are looking to have a backup. Any thoughts or suggestions would be greatly appreciated. Thanks , Bret Clough From vavalos <@t> allergydermatology.com Tue Dec 6 10:23:33 2011 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Tue Dec 6 10:23:46 2011 Subject: [Histonet] thermo excelsior es In-Reply-To: <8CE81CD3C58B0DA-C7C-E49C@webmail-d133.sysops.aol.com> References: <8CE81CD3C58B0DA-C7C-E49C@webmail-d133.sysops.aol.com> Message-ID: I love our excelsior. Our previous processor was an ancient VIP. We have had it for over 5 years. Pros: -You save on chemical if you program your chemicals to rotate based on the first Alcohol quality. It really works, I have tested my alcohols to make sure they were at the correct percentages and they were. -I also run a xylene free process (except for the flush). -We purchase the warranty every year and that includes a any maintenance parts needed, one PM, and labor. -The tech support service does everything they can over the phone before sending anyone out. -We have a local service tech now but if not parts are overnighted and a tech is sent out ASAP if tech support is unable to help you remedy the problem. Cons: - Not much other than I was not able to attend the training class. I know there is much more I could learn from it. - If you are in a building that has frequent power outages the back up battery does need to be replaced more often but that should be included in the warranty. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Sent: Monday, December 05, 2011 6:27 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] thermo excelsior es what are the disadvantages and advantages of excelsior ES tissue processor? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Tue Dec 6 10:34:10 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Dec 6 10:34:18 2011 Subject: [Histonet] Looking for a very good refurbished rotary microtome to purchase. In-Reply-To: References: Message-ID: Bret - just google 'used laboratory equipment" and you'll find a bunch of vendors.... For example- http://minnesotamedical.com/minnesotamedical/index.php?main_page=index&cPath=205_153 http://www.labx.com/v2/newad.cfm?catid=125 Cheers, Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clough, Bret Sent: Tuesday, December 06, 2011 11:19 AM To: Histonet list serv. Subject: [Histonet] Looking for a very good refurbished rotary microtome to purchase. I am trying to locate a really good inexpensive rotary microtome to purchase for our research lab. Any thoughts as to where to look and what model to get? We currently have a Lieca 1512 and are looking to have a backup. Any thoughts or suggestions would be greatly appreciated. Thanks , Bret Clough _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From relia1 <@t> earthlink.net Tue Dec 6 10:39:53 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Dec 6 10:39:57 2011 Subject: [Histonet] RELIA Special Management Careers Bulletin. Happy Holidays!! 12-06-2011 Message-ID: Hi Histonetters!! Happy Holidays!! While a job change is the last thing on most people?s minds this time of year; May I take a minute of your time to ask is a job change the next logical step in your career? This is a special bulletin for you. Some of my best clients are expanding and as a result some management opportunities are open right now and I wanted to touch base and give you a heads up. Your next opportunity might be just around the corner and I might have it for you. If you are looking for a position right now please contact me right away!! We can talk about my current positions OR about a customized search on your behalf. If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out that would be great too. To do that, just shoot me an email at relia1@earthlink.net or call me toll free at 866-607-3542. Here is a list of my current managerial opportunities: * Nighttime Supervisor ? Miami, FL** * Histology Lab Manager - Orlando, FL * Histology Supervisor - Orlando, FL * Histology Supervisor - Baton Rouge, LA * Histology Lab Manager - Modesto, CA * Histology Manager ? Long Island, New York * Histology Manager - Suffern, NY * Regional Histology Supervisor ? Portland, OR All of these clients offer autonomy in your position and histology staff eager to welcome their new manager. **While all of these clients are willing to hire right away; they are also willing to start the process with a phone interview now and continue the process after the holidays if that would be better for you! Remember, I offer a 500.00 dollar referral fee if I place someone you refer to me. I really appreciate you taking time out of your busy day to read my e-mail Thanks Again! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From dliaros <@t> BioReference.com Tue Dec 6 10:38:58 2011 From: dliaros <@t> BioReference.com (Donna Liaros) Date: Tue Dec 6 10:40:49 2011 Subject: [Histonet] Vendor recommendations for IHC Controls Message-ID: <6CB019913E5F6940AD6E4871841EC5360C5ECF3A@MBX-4.biosvr1.bioreference.com> Hi- Other than American Mastertech, Cell Marque and Newcomer Supply, what vendor would you recommend for IHC controls? I am looking for a supplier who can provide me good controls for Adenovirus and PMS2. Thanks! The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. From rjbuesa <@t> yahoo.com Tue Dec 6 10:49:39 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 6 10:49:45 2011 Subject: [Histonet] Looking for a very good refurbished rotary microtome to purchase. In-Reply-To: Message-ID: <1323190179.97668.YahooMailClassic@web65702.mail.ac4.yahoo.com> Check eBay. Sometimes they have good microtomes listed. Ren? J. --- On Tue, 12/6/11, Clough, Bret wrote: From: Clough, Bret Subject: [Histonet] Looking for a very good refurbished rotary microtome to purchase. To: "Histonet list serv." Date: Tuesday, December 6, 2011, 11:19 AM I am trying to locate a really good inexpensive rotary microtome? to purchase for our research lab. Any thoughts as to where to look and what model to get? We currently have a Lieca 1512 and are looking to have a backup. Any thoughts or suggestions would be greatly appreciated. Thanks , ? ? Bret Clough _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Clough <@t> medicine.tamhsc.edu Tue Dec 6 11:59:26 2011 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Tue Dec 6 11:59:59 2011 Subject: [Histonet] Trying to get in touch with Gayle Callis. Message-ID: I would like to get in touch with Gayle Callis but she is no longer at Montana State University. Does anyone know how to reach her and if so, would you be willing to share that information with me or have her contact me? Thanks, Bret Clough Texas A&M Health Science Center Temple, TX. From victor <@t> pathology.washington.edu Tue Dec 6 12:04:14 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Dec 6 12:07:56 2011 Subject: [Histonet] Trying to get in touch with Gayle Callis. In-Reply-To: References: Message-ID: <4EDE591E.3040400@pathology.washington.edu> gayle.callis@bresnan.net Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 12/6/2011 9:59 AM, Clough, Bret wrote: > I would like to get in touch with Gayle Callis but she is no longer at Montana State University. Does anyone know how to reach her and if so, would you be willing to share that information with me or have her contact me? > > Thanks, > Bret Clough > Texas A&M Health Science Center > Temple, TX. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Elizabeth.Dickert <@t> hcahealthcare.com Tue Dec 6 12:10:27 2011 From: Elizabeth.Dickert <@t> hcahealthcare.com (Elizabeth.Dickert@hcahealthcare.com) Date: Tue Dec 6 12:10:33 2011 Subject: [Histonet] SOX11 Message-ID: <0226177269DFDE48A23F40D00634258B15F41D1DD8@NADCWPMSGCMS10.hca.corpad.net> Having problem getting abcam sox11 to work, tried abcams recommended dilutions and several more with no luck! I need the secret!! E. Ann Dickert Southern Pathology 2333 McCallie Ave. Chattanooga, TN. 37404 phone 423.493.6904 fax 432.698.7858 From DKnutson <@t> primecare.org Tue Dec 6 14:30:14 2011 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Tue Dec 6 14:30:20 2011 Subject: [Histonet] Ventana NexES Special Stainer Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C8FE1@EXCHANGE2K7.staprimecare.org> We are moving our special stains bench from manual to automation with the Ventana NexES Special Stainer. I would be interested to hear from other Ventana users. How do you run your controls? Do you stain a control with each stain batch? Or one a day? I can't find any CAP standards on this. Another question I would like to ask other Ventana users - have you experienced any "down time" with your special stainer? Do you keep reagents handy to run manually when you are down? Thank you ahead of time for sharing your experiences with me! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From dmlongoria <@t> ecrmc.org Tue Dec 6 15:11:01 2011 From: dmlongoria <@t> ecrmc.org (Diana Martinez-Longoria) Date: Tue Dec 6 15:11:12 2011 Subject: [Histonet] seeking slide handles for racks In-Reply-To: References: Message-ID: Hello all, We have desperately seeking handles for our racks that are from Sakura Tissue Tek Slide Baskets 4768. My supervisor has gone to the Sakura website and has not any information about them. We only need the handles since we already have about 30 baskets. Please help. Thanking you guys in advance! Diana Martinez-Longoria Histotechnician (ASCP)cm El Centro Regional Medical Center phone: 760-339-7267 fax: 760-482-5365 email:dmlongoria@ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From MLunetta <@t> luhcares.org Wed Dec 7 07:01:31 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Dec 7 07:01:51 2011 Subject: [Histonet] Histo Aide Duties Message-ID: <4EDF013B020000A80006D511@ns.luhcares.org> Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) From MLunetta <@t> luhcares.org Wed Dec 7 07:52:33 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Dec 7 07:52:55 2011 Subject: [Histonet] Non-Certified Histo Aide Message-ID: <4EDF0D31020000A80006D526@ns.luhcares.org> Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) From one_angel_secret <@t> yahoo.com Wed Dec 7 09:25:37 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Dec 7 09:25:49 2011 Subject: [Histonet] Non-Certified Histo Aide In-Reply-To: <4EDF0D31020000A80006D526@ns.luhcares.org> References: <4EDF0D31020000A80006D526@ns.luhcares.org> Message-ID: <1323271537.56006.YahooMailNeo@web112305.mail.gq1.yahoo.com> You're probaly going to get a variety response to this depending on the state the person is in. Remember that CAP is an acreditation organization made up of peers that help each other stay within the guidlines of other state or federal regulatory agencies, such as CLIA and AHCA. < My version of the definition. ? This question was ask in the march addition of CAP. Link here: http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf? ? CAP accreditation = CMS $ from my understanding as well. http://wwwn.cdc.gov/clia/docs/fr12se01n.htm? Good article about this relationship ? For states such as the one I am in. Florida, only florida licensed histotechs can cut, embed, special stains, etc. to my knowledge in a hospital setting.I beleive there is an ecception for private dermatology labs performing MOHS. My understanding is that IHC and grossing are still high complex and is held to more stringent regulations. Someone correct me if i am wrong please. ? So I would see if your staff meet CLIA guidelines for moderatly complex testing and then look at your states requirements. ? Heres the guidelines on standard technical help. http://wwwn.cdc.gov/clia/docs/fr12se01n.htm ? The CLIA web site is so much fun to navigate, so I hope this answers some of your questions or at least gives you some reference points. ? Kim Donadio ? ? ? ________________________________ From: Matthew Lunetta To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 7, 2011 8:52 AM Subject: [Histonet] Non-Certified Histo Aide Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Dec 7 09:27:00 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Dec 7 09:27:10 2011 Subject: [Histonet] Lillie, 3rd Edition Message-ID: <02C099024072804EA34F5906BAC30A41062C17@nmdamailsvr.nmda.ad.nmsu.edu> In honor of my impending RETIREMENT on April 1, 2012, I have decided to offer for sale my copy of Histopathologic Technic and Practical Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and in very good condition; pages unbent, minor page darkening (age) and comes with a FREE autograph in my maiden name. First offer of eighty dollars ($80.00) takes it and I'll pay the postage to get it to you. I've got to pay for my bon-bons and TV cable bill somehow! First offer gets it. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From Kristopher.Kalleberg <@t> unilever.com Wed Dec 7 09:54:32 2011 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Wed Dec 7 09:54:38 2011 Subject: [Histonet] elastic fiber stain in dermis of skin Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0CA6D747@NTRSEVS30002.s3.ms.unilever.com> I am currently running a study to determine the increase in elastin in the dermis of skin due to certain treatments applied to skin. These samples will be FFPE after the study concludes. Does anyone have suggestions on which type of elastin stain works best for FFPE skin samples? Are there any commercially available stains that work well and are ready to use? Any help would be greatly appreciated. Thank you in advance. Kris From rjbuesa <@t> yahoo.com Wed Dec 7 09:54:55 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 7 09:54:59 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <4EDF013B020000A80006D511@ns.luhcares.org> Message-ID: <1323273295.32252.YahooMailClassic@web65716.mail.ac4.yahoo.com> Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 7 10:00:19 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 7 10:00:27 2011 Subject: [Histonet] elastic fiber stain in dermis of skin In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C0CA6D747@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <1323273619.94583.YahooMailClassic@web65714.mail.ac4.yahoo.com> Use the van Gieson stain. Just make sure that ALL the reagents are fresh (specially the iron hematoxylin). Try ARTERY as a (+) control. Ren? J. --- On Wed, 12/7/11, Kalleberg, Kristopher wrote: From: Kalleberg, Kristopher Subject: [Histonet] elastic fiber stain in dermis of skin To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 10:54 AM I am currently running a study to determine the increase in elastin in the dermis of skin due to certain treatments applied to skin.? These samples will be FFPE after the study concludes.? Does anyone have suggestions on which type of elastin stain works best for FFPE skin samples?? Are there any commercially available stains that work well and are ready to use?? Any help would be greatly appreciated.? Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From we3smitty <@t> yahoo.com Wed Dec 7 10:16:05 2011 From: we3smitty <@t> yahoo.com (angela smith) Date: Wed Dec 7 10:16:13 2011 Subject: [Histonet] IL-5 Message-ID: <1323274565.63625.YahooMailClassic@web125406.mail.ne1.yahoo.com> Does anyone know of a contract lab or facility that is performing IL-5 IHC on paraffin embedded tissue?? I have contacted US Labs, Labcorp and Genzyme and they do not. Thank you Angela Smith Histology Supervisor Children's Mercy Hospital ? From cbrya <@t> lexclin.com Wed Dec 7 10:17:50 2011 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Wed Dec 7 10:17:59 2011 Subject: [Histonet] adding eosin to tissue Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC376B3@EXCHANGESB> What are the pros and cons of adding eosin to tissue as it is processed to increase visibility when embedding with small specimens? Thank you in advance for your input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From flnails <@t> texaschildrens.org Wed Dec 7 10:25:31 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Dec 7 10:25:41 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <1323273295.32252.YahooMailClassic@web65716.mail.ac4.yahoo.com> References: <4EDF013B020000A80006D511@ns.luhcares.org> <1323273295.32252.YahooMailClassic@web65716.mail.ac4.yahoo.com> Message-ID: This sounds like union rules not CAP rules -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 07, 2011 9:55 AM To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu; Matthew Lunetta Subject: Re: [Histonet] Histo Aide Duties Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From nelsonrnch <@t> verizon.net Wed Dec 7 10:25:33 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Wed Dec 7 10:25:42 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <1323273295.32252.YahooMailClassic@web65716.mail.ac4.yahoo.com> References: <4EDF013B020000A80006D511@ns.luhcares.org> <1323273295.32252.YahooMailClassic@web65716.mail.ac4.yahoo.com> Message-ID: <1323275133.35545.YahooMailNeo@web84516.mail.ne1.yahoo.com> Rene could you please explain why coverslipping is a technical skill. It would seem coverslipping slides would be like labeling slides with just little bit more skill. Thank you in advance for your thoughts. ? HAPPY HOLIDAYS, ? PATTI RUBEN-NELSON? H.T.(ASCP) SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu; Matthew Lunetta Sent: Wednesday, December 7, 2011 7:54 AM Subject: Re: [Histonet] Histo Aide Duties Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Wed Dec 7 10:26:29 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Dec 7 10:26:45 2011 Subject: [Histonet] adding eosin to tissue In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC376B3@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC376B3@EXCHANGESB> Message-ID: <4EDF93B5.2050609@vneubert.com> I cannot see cons right now, but histoland: please feel free to correct me. Eosin is soluble in water, and as dewaxing most certainly will end in water, the eosin will be washed out by 70% EtOH and distilled water. Check your eosin to be sure. Am 07.12.2011 17:17, schrieb Carol Bryant: > What are the pros and cons of adding eosin to tissue as it is processed to increase visibility when embedding with small specimens? > Thank you in advance for your input. > > Carol Bryant, CT (ASCP) > Cytology/Histology Manager > Lexington Clinic > Phone (859) 258-4082 > Fax (859) 258-4081 > cbrya@lexclin.com > > > > NOTICE OF CONFIDENTIALITY > > This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 7 11:10:11 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 7 11:10:19 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <1323275133.35545.YahooMailNeo@web84516.mail.ne1.yahoo.com> Message-ID: <1323277811.11875.YahooMailClassic@web65706.mail.ac4.yahoo.com> Cover-slipping involves several decisions about size of the covers-lip, cleaning excess mounting medium and the like. Is the final step in the preparation of the slide and could also include a quality control about the staining that could result in returning the slide to re-stain. That type of decision involves looking the section under the microscope and that is absolutely outside the qualification of an aide. Ren? J. --- On Wed, 12/7/11, SHANE NELSON wrote: From: SHANE NELSON Subject: Re: [Histonet] Histo Aide Duties To: "Histonet" Cc: "rjbuesa@yahoo.com" Date: Wednesday, December 7, 2011, 11:25 AM Rene could you please explain why coverslipping is a technical skill. It would seem coverslipping slides would be like labeling slides with just little bit more skill. Thank you in advance for your thoughts. ? HAPPY HOLIDAYS, ? PATTI RUBEN-NELSON? H.T.(ASCP) SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu; Matthew Lunetta Sent: Wednesday, December 7, 2011 7:54 AM Subject: Re: [Histonet] Histo Aide Duties Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Dec 7 11:14:51 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Dec 7 11:13:58 2011 Subject: [Histonet] Lillie, 3rd Edition In-Reply-To: <02C099024072804EA34F5906BAC30A41062C17@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062C17@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4EDF9F0B.3020801@umdnj.edu> I wonder what my 4th edition (1976) is worth? Geoff On 12/7/2011 10:27 AM, Breeden, Sara wrote: > In honor of my impending RETIREMENT on April 1, 2012, I have decided to > offer for sale my copy of Histopathologic Technic and Practical > Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and > in very good condition; pages unbent, minor page darkening (age) and > comes with a FREE autograph in my maiden name. First offer of eighty > dollars ($80.00) takes it and I'll pay the postage to get it to you. > I've got to pay for my bon-bons and TV cable bill somehow! > > > > First offer gets it. > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Amanda <@t> YPII.com Wed Dec 7 11:26:14 2011 From: Amanda <@t> YPII.com (Mandy O'Connor) Date: Wed Dec 7 11:26:24 2011 Subject: [Histonet] Histo Aide Duties Message-ID: <8287D830EC5C904AA3E482C75773A03B0A0ECBBB@EX1.YPIILab.YPII.com> Hi guys and gals, I have to comment on this subject. This is where I wish there was a clear cut answer in deciphering what duties an aide vs. HT vs. HTL should and shouldn't do. I work in a private pathology lab where we have a little of everything-an HTL with BS in science, an HT with a 2 yr, an HT with no education, and an aide with only a couple college credits. Everyone is expected to know everything-embedding, cutting, staining, IHC, and also grossing. I highly do not agree with the expectations. Is there some documentation that actually says what the limits are for unqualified people? Also we need to question if they "should" be doing these duties. Mandy O'Connor, HTL(ASCP) Yellowstone Pathology Institute Billings, MT amanda@ypii.com From FUNKM <@t> mercyhealth.com Wed Dec 7 11:27:12 2011 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Dec 7 11:27:22 2011 Subject: [Histonet] Coverslipper Message-ID: <4EDF4D90020000AC00009FCE@nodcdmg2.no.trinity-health.org> Hello from the North land- We are having coverslipper issues with our Lecia. We have noticed that they coverslipes are not coming in clean as before and dropping off the red cups. We have changed the red cups but I have a concern with the dirty and dusty cover slips. If you have a minute please share what brand you are using. Thanks Much - Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 From kenp <@t> labx.com Wed Dec 7 11:32:44 2011 From: kenp <@t> labx.com (Ken Piech) Date: Wed Dec 7 11:33:27 2011 Subject: [Histonet] Looking for a very good refurbished rotary microtome to purchase. In-Reply-To: <1323190179.97668.YahooMailClassic@web65702.mail.ac4.yahoo.com> References: <1323190179.97668.YahooMailClassic@web65702.mail.ac4.yahoo.com> Message-ID: <85e2bd2725a4c7fa54f76815bde7103a@mail.gmail.com> Check the Microtome category on LabX, we are a marketplace just like eBay but for lab professionals wanting to purchase new and used lab equipment mainly. http://www.labx.com/v2/newad.cfm?catID=39 You can post a free Equipment Wanted ad as well. Best Regards, Ken Piech www.labx.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: December-06-11 11:50 AM To: Histonet list serv.; BretClough Subject: Re: [Histonet] Looking for a very good refurbished rotary microtome to purchase. Check eBay. Sometimes they have good microtomes listed. Ren? J. --- On Tue, 12/6/11, Clough, Bret wrote: From: Clough, Bret Subject: [Histonet] Looking for a very good refurbished rotary microtome to purchase. To: "Histonet list serv." Date: Tuesday, December 6, 2011, 11:19 AM I am trying to locate a really good inexpensive rotary microtome? to purchase for our research lab. Any thoughts as to where to look and what model to get? We currently have a Lieca 1512 and are looking to have a backup. Any thoughts or suggestions would be greatly appreciated. Thanks , ? ? Bret Clough _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Wed Dec 7 11:35:47 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Dec 7 11:35:55 2011 Subject: [Histonet] Lillie, 3rd Edition In-Reply-To: <4EDF9F0B.3020801@umdnj.edu> References: <02C099024072804EA34F5906BAC30A41062C17@nmdamailsvr.nmda.ad.nmsu.edu>, <4EDF9F0B.3020801@umdnj.edu> Message-ID: I just checked and Amazon has used, good condition, hard cover for $6.00. No matter the cost, the book is priceless if you work in a Histology lab. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 7 Dec 2011 12:14:51 -0500 > From: mcauliff@umdnj.edu > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Lillie, 3rd Edition > > I wonder what my 4th edition (1976) is worth? > > Geoff > > On 12/7/2011 10:27 AM, Breeden, Sara wrote: > > In honor of my impending RETIREMENT on April 1, 2012, I have decided to > > offer for sale my copy of Histopathologic Technic and Practical > > Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and > > in very good condition; pages unbent, minor page darkening (age) and > > comes with a FREE autograph in my maiden name. First offer of eighty > > dollars ($80.00) takes it and I'll pay the postage to get it to you. > > I've got to pay for my bon-bons and TV cable bill somehow! > > > > > > > > First offer gets it. > > > > > > > > Sally Breeden, HT(ASCP) > > > > New Mexico Department of Agriculture > > > > Veterinary Diagnostic Services > > > > 1101 Camino de Salud NE > > > > Albuquerque, NM 87102 > > > > 505-383-9278 (Histology Lab) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Dec 7 11:37:21 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Dec 7 11:37:32 2011 Subject: [Histonet] Coverslipper In-Reply-To: <4EDF4D90020000AC00009FCE@nodcdmg2.no.trinity-health.org> References: <4EDF4D90020000AC00009FCE@nodcdmg2.no.trinity-health.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F64EF2@SMCMAIL01.somerset-healthcare.com> StatLab coverglass. It also comes in a 2oz size which is nice for the coverglass dispensers on the CV5030 that we have. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Wednesday, December 07, 2011 12:27 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper Hello from the North land- We are having coverslipper issues with our Lecia. We have noticed that they coverslipes are not coming in clean as before and dropping off the red cups. We have changed the red cups but I have a concern with the dirty and dusty cover slips. If you have a minute please share what brand you are using. Thanks Much - Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From victor <@t> pathology.washington.edu Wed Dec 7 11:39:07 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Dec 7 11:39:19 2011 Subject: [Histonet] Lillie, 3rd Edition In-Reply-To: References: <02C099024072804EA34F5906BAC30A41062C17@nmdamailsvr.nmda.ad.nmsu.edu>, <4EDF9F0B.3020801@umdnj.edu> Message-ID: <4EDFA4BB.4000700@pathology.washington.edu> But they're not autographed by you know who. Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 12/7/2011 9:35 AM, WILLIAM DESALVO wrote: > I just checked and Amazon has used, good condition, hard cover for $6.00. No matter the cost, the book is priceless if you work in a Histology lab. > > William DeSalvo, B.S., HTL(ASCP) > > > >> Date: Wed, 7 Dec 2011 12:14:51 -0500 >> From: mcauliff@umdnj.edu >> To: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Lillie, 3rd Edition >> >> I wonder what my 4th edition (1976) is worth? >> >> Geoff >> >> On 12/7/2011 10:27 AM, Breeden, Sara wrote: >>> In honor of my impending RETIREMENT on April 1, 2012, I have decided to >>> offer for sale my copy of Histopathologic Technic and Practical >>> Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and >>> in very good condition; pages unbent, minor page darkening (age) and >>> comes with a FREE autograph in my maiden name. First offer of eighty >>> dollars ($80.00) takes it and I'll pay the postage to get it to you. >>> I've got to pay for my bon-bons and TV cable bill somehow! >>> >>> >>> >>> First offer gets it. >>> >>> >>> >>> Sally Breeden, HT(ASCP) >>> >>> New Mexico Department of Agriculture >>> >>> Veterinary Diagnostic Services >>> >>> 1101 Camino de Salud NE >>> >>> Albuquerque, NM 87102 >>> >>> 505-383-9278 (Histology Lab) >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> >> -- >> -- >> ********************************************** >> Geoff McAuliffe, Ph.D. >> Neuroscience and Cell Biology >> Robert Wood Johnson Medical School >> 675 Hoes Lane, Piscataway, NJ 08854 >> voice: (732)-235-4583 >> mcauliff@umdnj.edu >> ********************************************** >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Dec 7 11:43:43 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Dec 7 11:43:47 2011 Subject: [Histonet] LILLIE Message-ID: <02C099024072804EA34F5906BAC30A41062C1D@nmdamailsvr.nmda.ad.nmsu.edu> Well, you Crafty Individuals who found a 3rd edition Lillie at Amazon or the like and for cheaper, make it necessary for me to withdraw my offer of sale. I can't sell it for $8 and I'm not sure even about $40 (those online prices). No hard feelings. Deal's off. And I wasn't trying to pull a fast one. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From amber.mckenzie <@t> gastrodocs.net Wed Dec 7 11:47:44 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Dec 7 11:46:33 2011 Subject: [Histonet] lab chairs In-Reply-To: <8287D830EC5C904AA3E482C75773A03B0A0ECBBB@EX1.YPIILab.YPII.com> References: <8287D830EC5C904AA3E482C75773A03B0A0ECBBB@EX1.YPIILab.YPII.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0656D5@JERRY.Gia.com> Where do you recommend buying lab chairs? I'm looking for some w/o arm rests, solid black or tan, on rollers, etc.. From rsrichmond <@t> gmail.com Wed Dec 7 11:47:50 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Dec 7 11:47:57 2011 Subject: [Histonet] Re: Lillie, 3rd Edition Message-ID: You'll get my copy of the third edition of Ralph D. Lillie's Histologic Technic and Practical Histochemistry when they pry it from my cold dead fingers. I wish I'd bought the fourth edition, late 1970's I think. It's a great rarity and I've seen very high prices for it. Bob Richmond Samurai Pathologist Knoxville TN From lblazek <@t> digestivespecialists.com Wed Dec 7 11:53:55 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Dec 7 11:53:32 2011 Subject: [Histonet] Lillie, 3rd Edition In-Reply-To: <4EDFA4BB.4000700@pathology.washington.edu> References: <02C099024072804EA34F5906BAC30A41062C17@nmdamailsvr.nmda.ad.nmsu.edu>, <4EDF9F0B.3020801@umdnj.edu> <4EDFA4BB.4000700@pathology.washington.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA01F31@IBMB7Exchange.digestivespecialists.com> That alone is worth her weight in gold. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, December 07, 2011 12:39 PM To: WILLIAM DESALVO Cc: histonet Subject: Re: [Histonet] Lillie, 3rd Edition But they're not autographed by you know who. Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 12/7/2011 9:35 AM, WILLIAM DESALVO wrote: > I just checked and Amazon has used, good condition, hard cover for $6.00. No matter the cost, the book is priceless if you work in a Histology lab. > > William DeSalvo, B.S., HTL(ASCP) > > > >> Date: Wed, 7 Dec 2011 12:14:51 -0500 >> From: mcauliff@umdnj.edu >> To: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Lillie, 3rd Edition >> >> I wonder what my 4th edition (1976) is worth? >> >> Geoff >> >> On 12/7/2011 10:27 AM, Breeden, Sara wrote: >>> In honor of my impending RETIREMENT on April 1, 2012, I have decided to >>> offer for sale my copy of Histopathologic Technic and Practical >>> Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and >>> in very good condition; pages unbent, minor page darkening (age) and >>> comes with a FREE autograph in my maiden name. First offer of eighty >>> dollars ($80.00) takes it and I'll pay the postage to get it to you. >>> I've got to pay for my bon-bons and TV cable bill somehow! >>> >>> >>> >>> First offer gets it. >>> >>> >>> >>> Sally Breeden, HT(ASCP) >>> >>> New Mexico Department of Agriculture >>> >>> Veterinary Diagnostic Services >>> >>> 1101 Camino de Salud NE >>> >>> Albuquerque, NM 87102 >>> >>> 505-383-9278 (Histology Lab) >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> >> -- >> -- >> ********************************************** >> Geoff McAuliffe, Ph.D. >> Neuroscience and Cell Biology >> Robert Wood Johnson Medical School >> 675 Hoes Lane, Piscataway, NJ 08854 >> voice: (732)-235-4583 >> mcauliff@umdnj.edu >> ********************************************** >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Dec 7 12:13:31 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Dec 7 12:13:36 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <1323277811.11875.YahooMailClassic@web65706.mail.ac4.yahoo.com> References: <1323275133.35545.YahooMailNeo@web84516.mail.ne1.yahoo.com>, <1323277811.11875.YahooMailClassic@web65706.mail.ac4.yahoo.com> Message-ID: I don't want to seem nit picky, but I tend to strongly agree with Rene's point about coverslipping. It is not really the act or task of coverslipping to me, but rather the fact that it is one of the last opportunities to assess the slide for technical quality, overall presentation, and information accuracy before passing out. I feel that this should involve microscopic assessment, and also the judgment that arrives from knowledge and experience to determine if the slide is acceptable. I don't think it would be reasonable to expect all of this to occur with a person who has not had the benefit of training. many errors could be allowed out of the lab if labels are just put on without really looking at the slides, just think of the impact on perception of quality, accuracy and competency of histology. Most times, it is the "oops" that seem to stay in memory, and not the bulk, which go out fine. Not worth it to me.Joelle http://www.linkedin.com/in/joelleweaver > Date: Wed, 7 Dec 2011 09:10:11 -0800 > From: rjbuesa@yahoo.com > To: Histonet@lists.utsouthwestern.edu; nelsonrnch@verizon.net > Subject: Re: [Histonet] Histo Aide Duties > CC: > > Cover-slipping involves several decisions about size of the covers-lip, cleaning excess mounting medium and the like. > Is the final step in the preparation of the slide and could also include a quality control about the staining that could result in returning the slide to re-stain. That type of decision involves looking the section under the microscope and that is absolutely outside the qualification of an aide. > Ren? J. > > --- On Wed, 12/7/11, SHANE NELSON wrote: > > > From: SHANE NELSON > Subject: Re: [Histonet] Histo Aide Duties > To: "Histonet" > Cc: "rjbuesa@yahoo.com" > Date: Wednesday, December 7, 2011, 11:25 AM > > > > > > Rene could you please explain why coverslipping is a technical skill. It would seem coverslipping slides would be like labeling slides with just little bit more skill. Thank you in advance for your thoughts. > > > HAPPY HOLIDAYS, > > PATTI RUBEN-NELSON H.T.(ASCP) > SUPERVISOR/DGC > P.O. BOX 412 > CABAZON, CA. 92230 > cell (909) 841-9761 > nelsonrnch@verizon.net > > > CONFIDENTIALITY NOTICE: > This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants > and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable > law. Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you are not > the addressee, please promptly delete this message and notify the sender of > the delivery error by e-mail or you may call 909-841-9761. > > > > > > From: Rene J Buesa > To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu; Matthew Lunetta > Sent: Wednesday, December 7, 2011 7:54 AM > Subject: Re: [Histonet] Histo Aide Duties > > Embedding NO > Cutting ABSOLUTELY NO > Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) > Cover-slipping ONLY with automatic instrument (slides in/out) > Sticking labels to hand written slides if the slides are not pre-written > Filing slides and blocks YES > Matching slides with paper work YES > Delivering slides to pathologists YES > Arranging blocks/slides to cut YES > Anything that does not involve a technical skill, training or certification. > A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times > Ren? J. > > --- On Wed, 12/7/11, Matthew Lunetta wrote: > > > From: Matthew Lunetta > Subject: [Histonet] Histo Aide Duties > To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu > Date: Wednesday, December 7, 2011, 8:01 AM > > > Hey Histo Netters, > For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? > Other than accessioning. > Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? > I am not fluent in CAP and would like to know what you all think. > Thanks, > Matt Lunetta > BS HT(ASCP) > > -----Inline Attachment Follows----- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Wed Dec 7 12:42:40 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Dec 7 12:42:44 2011 Subject: [Histonet] RE: Histonet Digest, Vol 97, Issue 9 In-Reply-To: <0963914e-3731-4669-9fc0-9c86ee4d4df9@DCPWPRTR01.mdanderson.edu> References: <0963914e-3731-4669-9fc0-9c86ee4d4df9@DCPWPRTR01.mdanderson.edu> Message-ID: Deanne, When using automation, you should always keep a short supply of your most requested reagents for manual staining on hand. You just never know when the instrument will break down. Automation is great in the long run, in the beginning though you may spend a bit more than expected, just because you are trying out the correct times on the automated stainer, and the doctors are getting used to the stain. You should also perform parallel testing for quality assurance. As for the Ventana, the PAS is good, so is the AFB, and GMS. The only issue I remember is that you have to run the GMS and AFB at separate times or on different modules. Not good if you do not have the space. Also, if you experience a power outage or the unit goes down, you cannot just pick up an finish by hand. The unit does not tell you where the slides are in the process when the machine stops. As for controls, it depends on the facility, but one per case/stain is the usual rule. The only time that is not happening is for batch run H.Pylori. Controls can be scarce for this stain, and running them in a batch with one control has worked. I personally do not like that because of legalities and personal preference. Remember the phrase "all controls are appropriate", what if it gets hung up in another doctor's office. Just my two cents. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 3 Date: Tue, 6 Dec 2011 14:30:14 -0600 From: "Knutson, Deanne" Subject: [Histonet] Ventana NexES Special Stainer To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C8FE1@EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="us-ascii" We are moving our special stains bench from manual to automation with the Ventana NexES Special Stainer. I would be interested to hear from other Ventana users. How do you run your controls? Do you stain a control with each stain batch? Or one a day? I can't find any CAP standards on this. Another question I would like to ask other Ventana users - have you experienced any "down time" with your special stainer? Do you keep reagents handy to run manually when you are down? Thank you ahead of time for sharing your experiences with me! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------ From brannon <@t> alliedsearchpartners.com Wed Dec 7 13:17:11 2011 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Wed Dec 7 13:17:29 2011 Subject: [Histonet] Histotechnician Needed for the 3rd Shift in New York, NY Message-ID: Allied Search Partners has an opening for a qualified applicant to work for a large organization in New York City. We are inviting the best of the best in the field of histology to apply for this unique opportunity! Position: Histotech Shift: Monday-Friday 12am (midnight) ? 8am. No Weekends! Permanent Position. Location: New York, NY (10016) ? One of the largest and busiest Anatomic Pathology labs in New York ? This organization sets the standard for the field of histology, and provides their team with a high level of clinical interaction ? The fully-automated Histology lab enables expedited processing, including a large volume of diversified specimens. ? The organization has an unparalleled pursuit of advanced technology and exceptional training. Please forward your resume for review. -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From trathborne <@t> somerset-healthcare.com Wed Dec 7 13:53:30 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Dec 7 13:53:44 2011 Subject: [Histonet] RE: Tissue Loss In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2DDD7E5A44@FWDCWPMSGCMS09.hca.corpad.net> References: <14B823F24E628E49BBFAD704E4BAB89A379B20@chimsx03.CHI.catholichealth.net> <4BF03F5404EBDE409AF9232DA74B9DED2DDD7E5A44@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F64FF9@SMCMAIL01.somerset-healthcare.com> Is there water left under the sections before they go into the oven? Are they left in the oven they same length as the other techs slides? I remember having a seasoned tech that was put off by questioning also. I tried a different approach by meeting with her privately and discussing the matter. By explaining that our first priority is the safety of the patient (that being the patient specimen) and getting her to realize that this was not a disciplinary matter, but was really a matter for PI (Process Improvement). I let the tech become involved in resolving the problem and take ownership of it. This process took a few weeks, and there were some frustrating times, but eventually the problem was resolved. I later did a PI report to show the measures taken to correct the problem, and presented at our department Quality meetings. In the end, the tech felt rewarded by having her work come up to the standards of the others, and seeing the chart that showed improvements helped too. I -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Tuesday, December 06, 2011 3:07 AM To: Judith_Pardue@memorial.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Loss When tissue falls off the slide it is usually poorly cut sections or a problem with under processing or bad tissue ( bones) GI biopsies should not be falling off if cut well and processed correctly. Keep an eye on how she is cutting, she may have a problem with her microtome ( are all screws tight and is the angle right?) Is she cutting too thick? Is she ribboning? If a tech cannot get a ribbon on a biopsy she is doing something wrong. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Monday, December 05, 2011 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Loss I would like some input on a situation I am faced with in our lab. We are constantly having tissue coming off the slide on GI bx's. This is coming from one tech, she is a senior tech and takes offense to questioning her about the problem. From what I can tell all of the techs are following the same protocal. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 judith_pardue@memorial.org Memorial Healthcare System To the world I'm one, to one I'm the world This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From gagnone <@t> KGH.KARI.NET Wed Dec 7 14:06:20 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Dec 7 14:06:30 2011 Subject: [Histonet] Leica/Jung 2030 Microtome Ergonomic Handle Extension Message-ID: I'm looking for vendors who sell microtome handle extensions (vendors welcome to respond to list or directly to me). Has anyone purchased these? I'm looking for contact information and details/costs. We need to make microtomy as ergonomic as possible for microtomists who have difficulty moving the wheel through its normal full range of motion on a manual rotary microtome (Leica/Jung 2030 and 2035). We are automating with motorized microtomes, but still have manual microtomes in use. The recommendation in the literature is for an extension for the large wheel handle. This would be either Z-shape, pistol-grip or other design, with the goal being bringing the handle 3-4 inches closer to the microtomist throughout the wheel's range of motion. Thanks in advance for any assistance, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From one_angel_secret <@t> yahoo.com Wed Dec 7 14:37:24 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Dec 7 14:37:28 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <8287D830EC5C904AA3E482C75773A03B0A0ECBBB@EX1.YPIILab.YPII.com> References: <8287D830EC5C904AA3E482C75773A03B0A0ECBBB@EX1.YPIILab.YPII.com> Message-ID: <1323290244.9244.YahooMailNeo@web112312.mail.gq1.yahoo.com> I guess my first reply was too confusing. It's a confusing subject when your dealing with multiple states to which some are goverened by a requirement for a state license and some are not. ? Hopfully this will clear it up or muddy it even more, not sure. ? Labs that are accredited and get??reimbursment from CMS have to follow CLIA 88 guidelines. ? The Federal Centers for Medicare and Medicaid Services (CMS) regulates all laboratory testing (except research) performed on patients in the United States through CLIA.8 CLIA provides a number of important patient protections, such as laboratory personnel standards, proficiency testing (PT), quality assessment and control requirements, and cytology testing standards. The level of personnel skill and training required by the CLIA regulations depends on the complexity level of the testing performed.9 Complexity levels include waived, moderate and high complexity. In order to perform laboratory testing of waived, moderate or high complexity tests, laboratory personnel must satisfy minimum standards for the level of testing they perform. The CLIA personnel qualifications for the three categories of tests are: ? Waived Testing: Standards: None. ? Moderate Complexity Testing: Standards: Minimum requirement is a high school diploma or equivalent and documented training for the testing performed.10 ? High Complexity Testing: Standards: Minimum requirement is an Associate degree, including 24 semester hours in science, and completion of either: (1) an accredited or approved clinical laboratory training program, or (2) three months laboratory training in the specialty(ies) in which the individual performs high complexity testing.11 The personnel ? ? Link for this and more info: http://www.ascp.org/pdf/StateLicensureofLaboratoryPersonnel.aspx ? Here's a good article that talks about the 3 levels of complexity: http://www.aad.org/member-tools-and-benefits/practice-management-resources/compliance/clia/clia-faqs? ? Here's how states that do not license thier histotechs do it. Tissue embedding, sectioning, and staining in Pathology are considered part of specimen preparation, not a laboratory test, and do not fall under CLIA. Macroscopic (gross) examinations of specimens must be performed by an individual qualified under ?493.1449(l)?? Note here: IHC and grossing of specimens are HIGH COMPLEXITY TESTING no matter where you are. ? https://www.cms.gov/CLIA/downloads/apcindex.pdf?? ^^^^^ ? So since this question was about a CAP approved lab and the reason to do so is to be CLIA compliant to accept GOV payments, you follow CLIA guidelines, and if your state has further guidelines like Florida does, you follow them too. ? Is your state a licensure state? Ask that question. If not, then the answer to your question changes. ? That's about my entire 40 cents lol ? ? ? Kim ________________________________ From: Mandy O'Connor To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, December 7, 2011 12:26 PM Subject: RE: [Histonet] Histo Aide Duties Hi guys and gals, I have to comment on this subject.? This is where I wish there was a clear cut answer in deciphering what duties an aide vs. HT vs. HTL should and shouldn't do.? I work in a private pathology lab where we have a little of everything-an HTL with BS in science, an HT with a 2 yr, an HT with no education, and an aide with only a couple college credits.? Everyone is expected to know everything-embedding, cutting, staining, IHC, and also grossing.? I highly do not agree with the expectations. Is there some documentation that actually says what the limits are for unqualified people?? Also we need to question if they "should" be doing these duties. Mandy O'Connor, HTL(ASCP) Yellowstone Pathology Institute Billings, MT amanda@ypii.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Wed Dec 7 14:45:58 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Dec 7 14:47:29 2011 Subject: [Histonet] Histo Aide Duties Message-ID: <1937.208.62.167.196.1323290758.squirrel@webmail.realpages.com> Most professional labs require a person who has completed an accrediated histology programs, but CLIA states that a person can work within a histology lab if they have a combination of 12hrs of college chemistry or biology. In any combination. Then that person needs to be trained in each area they will work in and be signed off on by the directions as being able to perform tasks. To say that an unlicensed person working in histology can not coverslip is silly. I agree that this is the last chance to verify stain quality as well as tissue quality, but, before licensure there were thousands of OJT employees filling our histology positions. Many of whom trained each of us to have a critical eye when we were students performing our internships. If the person is working in ur labs as an unlicensed assistant, you must of had some confidence in them or you would not have them working in your lab. The evolution and technical skill of our trade has been taught from one person to another, at a time when licensure was not as important. That being said, licensure is now becomming critial to work in our field. But that does not mean that currecntly there are many unlicensed competent persons working in histology. They work as Mohs techs , and prep techs, and gross techs, etc. I would hope that a nonlicensed Mohs tech would know how to access their slides and coverslip effectively. My point, all persons working in our field contribute and get the job done, despite their licensure situations. They could still be very competent individuals if training by a patient person who is willing to educate others. Nicole Tatum HT ASCP ASMS > I don't want to seem nit picky, but I tend to strongly agree with Rene's > point about coverslipping. It is not really the act or task of > coverslipping to me, but rather the fact that it is one of the last > opportunities to assess the slide for technical quality, overall > presentation, and information accuracy before passing out. I feel that > this should involve microscopic assessment, and also the judgment that > arrives from knowledge and experience to determine if the slide is > acceptable. I don't think it would be reasonable to expect all of this to > occur with a person who has not had the benefit of training. many errors > could be allowed out of the lab if labels are just put on without really > looking at the slides, just think of the impact on perception of quality, > accuracy and competency of histology. Most times, it is the "oops" that > seem to stay in memory, and not the bulk, which go out fine. Not worth it > to me.Joelle > > http://www.linkedin.com/in/joelleweaver > > > Date: Wed, 7 Dec From rjbuesa <@t> yahoo.com Wed Dec 7 15:47:19 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 7 15:47:24 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <1937.208.62.167.196.1323290758.squirrel@webmail.realpages.com> Message-ID: <1323294439.8349.YahooMailClassic@web65715.mail.ac4.yahoo.com> I think that using the qualifier "silly" is not called for. Regardless of how things were done years ago it does not mean that they ought to continue that way. As the last step I personally think (even if you think it is "silly") that?cover-slipping should NOT be completed by an unlicensed person with not enough knowledge for the task. An aide is just that, an aide that can be very helpful in completing some tasks efficiently and cheaper than a HT, but that ought not to be allowed to do technical tasks. As to automated cover-slippers go?the final product should be inspected by a licensed HT before sending it to the pathologists. It is just a way of increasing productivity and cutting costs. Regardless of all, a lab is run by a supervisor and she or he is the one who decides what tasks can be done by an aide, even if he or she ends as being considered "silly". Ren? J. --- On Wed, 12/7/11, Nicole Tatum wrote: From: Nicole Tatum Subject: RE: [Histonet] Histo Aide Duties To: "joelle weaver" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 3:45 PM Most professional labs require a person who has completed an accrediated histology programs, but CLIA states that a person can work within a histology lab if they have a combination of 12hrs of college chemistry or biology. In any combination. Then that person needs to be trained in each area they will work in and be signed off on by the directions as being able to perform tasks. To say that an unlicensed person working in histology can not coverslip is silly. I agree that this is the last chance to verify stain quality as well as tissue quality, but, before licensure there were thousands of OJT employees filling our histology positions. Many of whom trained each of us to have a critical eye when we were students performing our internships. If the person is working in ur labs as an unlicensed assistant, you must of had some confidence in them or you would not have them working in your lab.? The evolution and technical skill of our trade has been taught from one person to another, at a time when licensure was not as important. That being said, licensure is now becomming critial to work in our field. But that does not mean that currecntly there are many unlicensed competent persons working in histology. They work as Mohs techs , and prep techs, and gross techs, etc. I would hope that a nonlicensed Mohs tech would know how to access their slides and coverslip effectively. My point, all persons working in our field contribute and get the job done, despite their licensure situations. They could still be very competent individuals if training by a patient person who is willing to educate others. Nicole Tatum HT ASCP ASMS > I don't want to seem nit picky, but I tend to strongly agree with Rene's > point about coverslipping. It is not really the act or task of > coverslipping to me, but rather the fact that it is one of the last > opportunities to assess the slide for technical quality, overall > presentation, and information accuracy before passing out. I feel that > this should involve microscopic assessment, and also the judgment that > arrives from knowledge and experience to determine if the slide is > acceptable. I don't think it would be reasonable to expect all of this to > occur with a person who has not had the benefit of training. many errors > could be allowed out of the lab if labels are just put on without really > looking at the slides, just think of the impact on perception of quality, > accuracy and competency of histology. Most times, it is the "oops" that > seem to stay in memory, and not the bulk,? which go out fine. Not worth it > to me.Joelle > > http://www.linkedin.com/in/joelleweaver > >? > Date: Wed, 7 Dec _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFoshey <@t> chw.org Wed Dec 7 15:57:04 2011 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Wed Dec 7 16:00:29 2011 Subject: [Histonet] RE: Histonet Digest, Vol 97, Issue 9 In-Reply-To: <28c64480-af87-4370-9eb4-27331ec97f3d@c1excpws04.chwi.chswi.org> References: <28c64480-af87-4370-9eb4-27331ec97f3d@c1excpws04.chwi.chswi.org> Message-ID: Histonetters: I'm looking for a pediatric institution that charges for autopsy services. What is the charge for a routine pediatric autopsy? What is your charge for a complex autopsy? Thanks in advance for your responses, Annette Foshey, HT Histology Department Children's Hospital of Wisconsin ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Wednesday, December 07, 2011 11:43 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 97, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Trying to get in touch with Gayle Callis. (Victor Tobias) 2. SOX11 (Elizabeth.Dickert@hcahealthcare.com) 3. Ventana NexES Special Stainer (Knutson, Deanne) 4. seeking slide handles for racks (Diana Martinez-Longoria) 5. Histo Aide Duties (Matthew Lunetta) 6. Non-Certified Histo Aide (Matthew Lunetta) 7. Re: Non-Certified Histo Aide (Kim Donadio) 8. Lillie, 3rd Edition (Breeden, Sara) 9. elastic fiber stain in dermis of skin (Kalleberg, Kristopher) 10. Re: Histo Aide Duties (Rene J Buesa) 11. Re: elastic fiber stain in dermis of skin (Rene J Buesa) 12. IL-5 (angela smith) 13. adding eosin to tissue (Carol Bryant) 14. RE: Histo Aide Duties (Nails, Felton) 15. Re: Histo Aide Duties (SHANE NELSON) 16. Re: adding eosin to tissue (V. Neubert) 17. Re: Histo Aide Duties (Rene J Buesa) 18. Re: Lillie, 3rd Edition (Geoff McAuliffe) 19. RE: Histo Aide Duties (Mandy O'Connor) 20. Coverslipper (Marcia Funk) 21. RE: Looking for a very good refurbished rotary microtome to purchase. (Ken Piech) 22. RE: Lillie, 3rd Edition (WILLIAM DESALVO) 23. RE: Coverslipper (Rathborne, Toni) ---------------------------------------------------------------------- Message: 1 Date: Tue, 06 Dec 2011 10:04:14 -0800 From: Victor Tobias Subject: Re: [Histonet] Trying to get in touch with Gayle Callis. To: histonet@lists.utsouthwestern.edu Message-ID: <4EDE591E.3040400@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed gayle.callis@bresnan.net Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 12/6/2011 9:59 AM, Clough, Bret wrote: > I would like to get in touch with Gayle Callis but she is no longer at Montana State University. Does anyone know how to reach her and if so, would you be willing to share that information with me or have her contact me? > > Thanks, > Bret Clough > Texas A&M Health Science Center > Temple, TX. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 6 Dec 2011 12:10:27 -0600 From: Subject: [Histonet] SOX11 To: Message-ID: <0226177269DFDE48A23F40D00634258B15F41D1DD8@NADCWPMSGCMS10.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Having problem getting abcam sox11 to work, tried abcams recommended dilutions and several more with no luck! I need the secret!! E. Ann Dickert Southern Pathology 2333 McCallie Ave. Chattanooga, TN. 37404 phone 423.493.6904 fax 432.698.7858 ------------------------------ Message: 3 Date: Tue, 6 Dec 2011 14:30:14 -0600 From: "Knutson, Deanne" Subject: [Histonet] Ventana NexES Special Stainer To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C8FE1@EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="us-ascii" We are moving our special stains bench from manual to automation with the Ventana NexES Special Stainer. I would be interested to hear from other Ventana users. How do you run your controls? Do you stain a control with each stain batch? Or one a day? I can't find any CAP standards on this. Another question I would like to ask other Ventana users - have you experienced any "down time" with your special stainer? Do you keep reagents handy to run manually when you are down? Thank you ahead of time for sharing your experiences with me! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------ Message: 4 Date: Tue, 6 Dec 2011 21:11:01 +0000 From: Diana Martinez-Longoria Subject: [Histonet] seeking slide handles for racks To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello all, We have desperately seeking handles for our racks that are from Sakura Tissue Tek Slide Baskets 4768. My supervisor has gone to the Sakura website and has not any information about them. We only need the handles since we already have about 30 baskets. Please help. Thanking you guys in advance! Diana Martinez-Longoria Histotechnician (ASCP)cm El Centro Regional Medical Center phone: 760-339-7267 fax: 760-482-5365 email:dmlongoria@ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? ------------------------------ Message: 5 Date: Wed, 07 Dec 2011 06:01:31 -0700 From: "Matthew Lunetta" Subject: [Histonet] Histo Aide Duties To: , Message-ID: <4EDF013B020000A80006D511@ns.luhcares.org> Content-Type: text/plain; charset="us-ascii" Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) ------------------------------ Message: 6 Date: Wed, 07 Dec 2011 06:52:33 -0700 From: "Matthew Lunetta" Subject: [Histonet] Non-Certified Histo Aide To: Message-ID: <4EDF0D31020000A80006D526@ns.luhcares.org> Content-Type: text/plain; charset="us-ascii" Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) ------------------------------ Message: 7 Date: Wed, 7 Dec 2011 07:25:37 -0800 (PST) From: Kim Donadio Subject: Re: [Histonet] Non-Certified Histo Aide To: Matthew Lunetta , "histonet@lists.utsouthwestern.edu" Message-ID: <1323271537.56006.YahooMailNeo@web112305.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You're probaly going to get a variety response to this depending on the state the person is in. Remember that CAP is an acreditation organization made up of peers that help each other stay within the guidlines of other state or federal regulatory agencies, such as CLIA and AHCA. < My version of the definition. ? This question was ask in the march addition of CAP. Link here: http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf? ? CAP accreditation = CMS $ from my understanding as well. http://wwwn.cdc.gov/clia/docs/fr12se01n.htm? Good article about this relationship ? For states such as the one I am in. Florida, only florida licensed histotechs can cut, embed, special stains, etc. to my knowledge in a hospital setting.I beleive there is an ecception for private dermatology labs performing MOHS. My understanding is that IHC and grossing are still high complex and is held to more stringent regulations. Someone correct me if i am wrong please. ? So I would see if your staff meet CLIA guidelines for moderatly complex testing and then look at your states requirements. ? Heres the guidelines on standard technical help. http://wwwn.cdc.gov/clia/docs/fr12se01n.htm ? The CLIA web site is so much fun to navigate, so I hope this answers some of your questions or at least gives you some reference points. ? Kim Donadio ? ? ? ________________________________ From: Matthew Lunetta To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 7, 2011 8:52 AM Subject: [Histonet] Non-Certified Histo Aide Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 7 Dec 2011 08:27:00 -0700 From: "Breeden, Sara" Subject: [Histonet] Lillie, 3rd Edition To: Message-ID: <02C099024072804EA34F5906BAC30A41062C17@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" In honor of my impending RETIREMENT on April 1, 2012, I have decided to offer for sale my copy of Histopathologic Technic and Practical Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and in very good condition; pages unbent, minor page darkening (age) and comes with a FREE autograph in my maiden name. First offer of eighty dollars ($80.00) takes it and I'll pay the postage to get it to you. I've got to pay for my bon-bons and TV cable bill somehow! First offer gets it. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ Message: 9 Date: Wed, 7 Dec 2011 10:54:32 -0500 From: "Kalleberg, Kristopher" Subject: [Histonet] elastic fiber stain in dermis of skin To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0CA6D747@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="US-ASCII" I am currently running a study to determine the increase in elastin in the dermis of skin due to certain treatments applied to skin. These samples will be FFPE after the study concludes. Does anyone have suggestions on which type of elastin stain works best for FFPE skin samples? Are there any commercially available stains that work well and are ready to use? Any help would be greatly appreciated. Thank you in advance. Kris ------------------------------ Message: 10 Date: Wed, 7 Dec 2011 07:54:55 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu, Matthew Lunetta Message-ID: <1323273295.32252.YahooMailClassic@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 7 Dec 2011 08:00:19 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] elastic fiber stain in dermis of skin To: histonet@lists.utsouthwestern.edu, KristopherKalleberg Message-ID: <1323273619.94583.YahooMailClassic@web65714.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Use the van Gieson stain. Just make sure that ALL the reagents are fresh (specially the iron hematoxylin). Try ARTERY as a (+) control. Ren? J. --- On Wed, 12/7/11, Kalleberg, Kristopher wrote: From: Kalleberg, Kristopher Subject: [Histonet] elastic fiber stain in dermis of skin To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 10:54 AM I am currently running a study to determine the increase in elastin in the dermis of skin due to certain treatments applied to skin.? These samples will be FFPE after the study concludes.? Does anyone have suggestions on which type of elastin stain works best for FFPE skin samples?? Are there any commercially available stains that work well and are ready to use?? Any help would be greatly appreciated.? Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 7 Dec 2011 08:16:05 -0800 (PST) From: angela smith Subject: [Histonet] IL-5 To: Histonet Histonet Message-ID: <1323274565.63625.YahooMailClassic@web125406.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Does anyone know of a contract lab or facility that is performing IL-5 IHC on paraffin embedded tissue?? I have contacted US Labs, Labcorp and Genzyme and they do not. Thank you Angela Smith Histology Supervisor Children's Mercy Hospital ? ------------------------------ Message: 13 Date: Wed, 7 Dec 2011 11:17:50 -0500 From: Carol Bryant Subject: [Histonet] adding eosin to tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC376B3@EXCHANGESB> Content-Type: text/plain; charset="us-ascii" What are the pros and cons of adding eosin to tissue as it is processed to increase visibility when embedding with small specimens? Thank you in advance for your input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ------------------------------ Message: 14 Date: Wed, 7 Dec 2011 10:25:31 -0600 From: "Nails, Felton" Subject: RE: [Histonet] Histo Aide Duties To: 'Rene J Buesa' , "histonet@lists.utsouthwestern.edu" , "histonet-request@lists.utsouthwestern.edu" , Matthew Lunetta Message-ID: Content-Type: text/plain; charset="iso-8859-1" This sounds like union rules not CAP rules -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 07, 2011 9:55 AM To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu; Matthew Lunetta Subject: Re: [Histonet] Histo Aide Duties Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ ------------------------------ Message: 15 Date: Wed, 7 Dec 2011 08:25:33 -0800 (PST) From: SHANE NELSON Subject: Re: [Histonet] Histo Aide Duties To: Histonet Message-ID: <1323275133.35545.YahooMailNeo@web84516.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Rene could you please explain why coverslipping is a technical skill. It would seem coverslipping slides would be like labeling slides with just little bit more skill. Thank you in advance for your thoughts. ? HAPPY HOLIDAYS, ? PATTI RUBEN-NELSON? H.T.(ASCP) SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu; Matthew Lunetta Sent: Wednesday, December 7, 2011 7:54 AM Subject: Re: [Histonet] Histo Aide Duties Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 07 Dec 2011 17:26:29 +0100 From: "V. Neubert" Subject: Re: [Histonet] adding eosin to tissue To: histonet@lists.utsouthwestern.edu Message-ID: <4EDF93B5.2050609@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 I cannot see cons right now, but histoland: please feel free to correct me. Eosin is soluble in water, and as dewaxing most certainly will end in water, the eosin will be washed out by 70% EtOH and distilled water. Check your eosin to be sure. Am 07.12.2011 17:17, schrieb Carol Bryant: > What are the pros and cons of adding eosin to tissue as it is processed to increase visibility when embedding with small specimens? > Thank you in advance for your input. > > Carol Bryant, CT (ASCP) > Cytology/Histology Manager > Lexington Clinic > Phone (859) 258-4082 > Fax (859) 258-4081 > cbrya@lexclin.com > > > > NOTICE OF CONFIDENTIALITY > > This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 7 Dec 2011 09:10:11 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Histo Aide Duties To: Histonet , SHANE NELSON Message-ID: <1323277811.11875.YahooMailClassic@web65706.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Cover-slipping involves several decisions about size of the covers-lip, cleaning excess mounting medium and the like. Is the final step in the preparation of the slide and could also include a quality control about the staining that could result in returning the slide to re-stain. That type of decision involves looking the section under the microscope and that is absolutely outside the qualification of an aide. Ren? J. --- On Wed, 12/7/11, SHANE NELSON wrote: From: SHANE NELSON Subject: Re: [Histonet] Histo Aide Duties To: "Histonet" Cc: "rjbuesa@yahoo.com" Date: Wednesday, December 7, 2011, 11:25 AM Rene could you please explain why coverslipping is a technical skill. It would seem coverslipping slides would be like labeling slides with just little bit more skill. Thank you in advance for your thoughts. ? HAPPY HOLIDAYS, ? PATTI RUBEN-NELSON? H.T.(ASCP) SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu; Matthew Lunetta Sent: Wednesday, December 7, 2011 7:54 AM Subject: Re: [Histonet] Histo Aide Duties Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 07 Dec 2011 12:14:51 -0500 From: Geoff McAuliffe Subject: Re: [Histonet] Lillie, 3rd Edition To: histonet@lists.utsouthwestern.edu Message-ID: <4EDF9F0B.3020801@umdnj.edu> Content-Type: text/plain; CHARSET=US-ASCII; format=flowed I wonder what my 4th edition (1976) is worth? Geoff On 12/7/2011 10:27 AM, Breeden, Sara wrote: > In honor of my impending RETIREMENT on April 1, 2012, I have decided to > offer for sale my copy of Histopathologic Technic and Practical > Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and > in very good condition; pages unbent, minor page darkening (age) and > comes with a FREE autograph in my maiden name. First offer of eighty > dollars ($80.00) takes it and I'll pay the postage to get it to you. > I've got to pay for my bon-bons and TV cable bill somehow! > > > > First offer gets it. > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 19 Date: Wed, 7 Dec 2011 17:26:14 +0000 From: Mandy O'Connor Subject: RE: [Histonet] Histo Aide Duties To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <8287D830EC5C904AA3E482C75773A03B0A0ECBBB@EX1.YPIILab.YPII.com> Content-Type: text/plain; charset="us-ascii" Hi guys and gals, I have to comment on this subject. This is where I wish there was a clear cut answer in deciphering what duties an aide vs. HT vs. HTL should and shouldn't do. I work in a private pathology lab where we have a little of everything-an HTL with BS in science, an HT with a 2 yr, an HT with no education, and an aide with only a couple college credits. Everyone is expected to know everything-embedding, cutting, staining, IHC, and also grossing. I highly do not agree with the expectations. Is there some documentation that actually says what the limits are for unqualified people? Also we need to question if they "should" be doing these duties. Mandy O'Connor, HTL(ASCP) Yellowstone Pathology Institute Billings, MT amanda@ypii.com ------------------------------ Message: 20 Date: Wed, 07 Dec 2011 12:27:12 -0500 From: "Marcia Funk" Subject: [Histonet] Coverslipper To: Message-ID: <4EDF4D90020000AC00009FCE@nodcdmg2.no.trinity-health.org> Content-Type: text/plain; charset=US-ASCII Hello from the North land- We are having coverslipper issues with our Lecia. We have noticed that they coverslipes are not coming in clean as before and dropping off the red cups. We have changed the red cups but I have a concern with the dirty and dusty cover slips. If you have a minute please share what brand you are using. Thanks Much - Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 ------------------------------ Message: 21 Date: Wed, 7 Dec 2011 12:32:44 -0500 From: Ken Piech Subject: RE: [Histonet] Looking for a very good refurbished rotary microtome to purchase. To: Rene J Buesa , "Histonet list serv." , BretClough Message-ID: <85e2bd2725a4c7fa54f76815bde7103a@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Check the Microtome category on LabX, we are a marketplace just like eBay but for lab professionals wanting to purchase new and used lab equipment mainly. http://www.labx.com/v2/newad.cfm?catID=39 You can post a free Equipment Wanted ad as well. Best Regards, Ken Piech www.labx.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: December-06-11 11:50 AM To: Histonet list serv.; BretClough Subject: Re: [Histonet] Looking for a very good refurbished rotary microtome to purchase. Check eBay. Sometimes they have good microtomes listed. Ren? J. --- On Tue, 12/6/11, Clough, Bret wrote: From: Clough, Bret Subject: [Histonet] Looking for a very good refurbished rotary microtome to purchase. To: "Histonet list serv." Date: Tuesday, December 6, 2011, 11:19 AM I am trying to locate a really good inexpensive rotary microtome? to purchase for our research lab. Any thoughts as to where to look and what model to get? We currently have a Lieca 1512 and are looking to have a backup. Any thoughts or suggestions would be greatly appreciated. Thanks , ? ? Bret Clough _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 7 Dec 2011 10:35:47 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] Lillie, 3rd Edition To: , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I just checked and Amazon has used, good condition, hard cover for $6.00. No matter the cost, the book is priceless if you work in a Histology lab. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 7 Dec 2011 12:14:51 -0500 > From: mcauliff@umdnj.edu > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Lillie, 3rd Edition > > I wonder what my 4th edition (1976) is worth? > > Geoff > > On 12/7/2011 10:27 AM, Breeden, Sara wrote: > > In honor of my impending RETIREMENT on April 1, 2012, I have decided to > > offer for sale my copy of Histopathologic Technic and Practical > > Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and > > in very good condition; pages unbent, minor page darkening (age) and > > comes with a FREE autograph in my maiden name. First offer of eighty > > dollars ($80.00) takes it and I'll pay the postage to get it to you. > > I've got to pay for my bon-bons and TV cable bill somehow! > > > > > > > > First offer gets it. > > > > > > > > Sally Breeden, HT(ASCP) > > > > New Mexico Department of Agriculture > > > > Veterinary Diagnostic Services > > > > 1101 Camino de Salud NE > > > > Albuquerque, NM 87102 > > > > 505-383-9278 (Histology Lab) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Wed, 7 Dec 2011 17:37:21 +0000 From: "Rathborne, Toni" Subject: RE: [Histonet] Coverslipper To: "'Marcia Funk'" , "Histonet@lists.utsouthwestern.edu" Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F64EF2@SMCMAIL01.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" StatLab coverglass. It also comes in a 2oz size which is nice for the coverglass dispensers on the CV5030 that we have. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Wednesday, December 07, 2011 12:27 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper Hello from the North land- We are having coverslipper issues with our Lecia. We have noticed that they coverslipes are not coming in clean as before and dropping off the red cups. We have changed the red cups but I have a concern with the dirty and dusty cover slips. If you have a minute please share what brand you are using. Thanks Much - Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 97, Issue 9 *************************************** From wdesalvo.cac <@t> hotmail.com Wed Dec 7 16:05:43 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Dec 7 16:05:47 2011 Subject: [Histonet] LILLIE In-Reply-To: <02C099024072804EA34F5906BAC30A41062C1D@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062C1D@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: No worries and no fast one assumed. There is no issue w/ anyone trying to create value and get what they can for a valuable item. The book, who offers the book and it's use will always provide value to the recipient. I stand by my previous statement -This book is a must for all Histology labs! William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 7 Dec 2011 10:43:43 -0700 > From: sbreeden@nmda.nmsu.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] LILLIE > > Well, you Crafty Individuals who found a 3rd edition Lillie at Amazon or > the like and for cheaper, make it necessary for me to withdraw my offer > of sale. I can't sell it for $8 and I'm not sure even about $40 (those > online prices). No hard feelings. Deal's off. And I wasn't trying to > pull a fast one. > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jennifer.Bull <@t> northwestpathology.com Wed Dec 7 16:05:44 2011 From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Wed Dec 7 16:05:51 2011 Subject: [Histonet] Looking for Brain Tissue with MS plaques Message-ID: <85760CECEC18444BB95F26D5E88DAEAA22EE81439F@hinet2.hinet.org> I'm looking for brain tissue containing ms plaques for use as a control for the Luxol Fast Blue Stain. Would anyone out there be willing to part with a block or know of somewhere I can order such control slides? Thanks for your help! Jenny mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- From Diane.Tokugawa <@t> kp.org Wed Dec 7 16:06:48 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Wed Dec 7 16:07:05 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 12/07/2011 and will not return until 12/08/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Client services 8-421-5408, Maria (5408), Mario at 8-421-4961, Kiran at 8-421-5404, or Wanda at 8-421-5426. From SDattili <@t> stormontvail.org Wed Dec 7 16:43:04 2011 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Wed Dec 7 16:43:09 2011 Subject: [Histonet] RE: Histology lab assistant In-Reply-To: <5a22b26000063d8f@stormontvail.org> Message-ID: Hi all, Matt asked about the duties of a non-certified Histology aide. We have had this position for about 4 years and I consider it absolutely vital to the running of the lab. We originally started with a half-time assistant working in the afternoons (the time with the busiest call volume). This summer, I was able to increase the hours and make the position full time, working 0830-1700. The assistant's responsibilities are as follows: * Filing slides and paperwork * Morning slide distribution to pathologists (match slides to reports, last check of number of parts submitted) * Answering the phone (a big boon to histotech productivity!) * Coordinates send-out testing--pulls slides and block and takes to pathologist, fill out paperwork, package, etc. * Moves slides from the stainer to the automatic coverslipper * Some non-technical tasks related to our weekly Tumor board preparation * Back-up gross room assistant for patient registration, specimen accessioning, and assist with gross exam * Compilation of some CAP-required statistics (e.g. frozen section turnaround times) * Reviewing bills and handling reference lab reports Eventually, this position may also be assigned minor daily maintenance on the slide labeler and H&E stainer and alcohol recycling. Giving these tasks to an assistant, especially answering the phone and managing sendouts, allows our histotechs to focus on their core competencies as well as other technical responsibilites such as regular procedure review and other items related to our CAP accreditation. Our professional staff is a mixture of HT(ASCP) and HTL(ASCP), with a few folks who are trained or in training. These people have the necessary education to sit for the certification exam following their training period and they are expected to pass the exam as a condition of their continued employment. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From one_angel_secret <@t> yahoo.com Wed Dec 7 16:47:18 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Dec 7 16:47:22 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <1323294439.8349.YahooMailClassic@web65715.mail.ac4.yahoo.com> References: <1937.208.62.167.196.1323290758.squirrel@webmail.realpages.com> <1323294439.8349.YahooMailClassic@web65715.mail.ac4.yahoo.com> Message-ID: <1323298038.71111.YahooMailNeo@web112310.mail.gq1.yahoo.com> I'm sure there are worse comments that could have been made to your "opinion" that someone should be licensed to coverslip. Obviously you realize the cost for labs for such an act? Coverslipping is not technical in my opinion. (or CLIA's and CAP's ) ? Yes, supervisors are mandated with the responsibility of making sure the product are of high quality. But they have regulations and guidelines they follow. Back to the CLIA/CAP guidelines. ? Quality of slides should be monitored with daily QC. Ive often done this by filling out a QC sheet with a certain % of cases to have a Pathologist sign off on. CAP loves this type of thing. ? It doesnt provide any of us any service to our profession to belittle those who help us, those aides. The aides of which I have had many who hold BS degrees in science feilds who do not have a certification or licensure as a Histotech. Many of whom could whip out a FNA cytospin with perfection all at the same time coverslipping a hundred more. ? Rene, I just think you shocked some of us with your opinion that coverslipping should be done by licensed personel. ? I hope you dont take any of this as personal. This subject should have just remained per the laws/regulations as it was ask. ? Things always get messy when we put our opinions in. ? Now I've put mine in and I'm running for the hills......................................... ? KD ________________________________ From: Rene J Buesa To: joelle weaver ; histonet@lists.utsouthwestern.edu; Nicole Tatum Sent: Wednesday, December 7, 2011 4:47 PM Subject: RE: [Histonet] Histo Aide Duties I think that using the qualifier "silly" is not called for. Regardless of how things were done years ago it does not mean that they ought to continue that way. As the last step I personally think (even if you think it is "silly") that?cover-slipping should NOT be completed by an unlicensed person with not enough knowledge for the task. An aide is just that, an aide that can be very helpful in completing some tasks efficiently and cheaper than a HT, but that ought not to be allowed to do technical tasks. As to automated cover-slippers go?the final product should be inspected by a licensed HT before sending it to the pathologists. It is just a way of increasing productivity and cutting costs. Regardless of all, a lab is run by a supervisor and she or he is the one who decides what tasks can be done by an aide, even if he or she ends as being considered "silly". Ren? J. --- On Wed, 12/7/11, Nicole Tatum wrote: From: Nicole Tatum Subject: RE: [Histonet] Histo Aide Duties To: "joelle weaver" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 3:45 PM Most professional labs require a person who has completed an accrediated histology programs, but CLIA states that a person can work within a histology lab if they have a combination of 12hrs of college chemistry or biology. In any combination. Then that person needs to be trained in each area they will work in and be signed off on by the directions as being able to perform tasks. To say that an unlicensed person working in histology can not coverslip is silly. I agree that this is the last chance to verify stain quality as well as tissue quality, but, before licensure there were thousands of OJT employees filling our histology positions. Many of whom trained each of us to have a critical eye when we were students performing our internships. If the person is working in ur labs as an unlicensed assistant, you must of had some confidence in them or you would not have them working in your lab.? The evolution and technical skill of our trade has been taught from one person to another, at a time when licensure was not as important. That being said, licensure is now becomming critial to work in our field. But that does not mean that currecntly there are many unlicensed competent persons working in histology. They work as Mohs techs , and prep techs, and gross techs, etc. I would hope that a nonlicensed Mohs tech would know how to access their slides and coverslip effectively. My point, all persons working in our field contribute and get the job done, despite their licensure situations. They could still be very competent individuals if training by a patient person who is willing to educate others. Nicole Tatum HT ASCP ASMS > I don't want to seem nit picky, but I tend to strongly agree with Rene's > point about coverslipping. It is not really the act or task of > coverslipping to me, but rather the fact that it is one of the last > opportunities to assess the slide for technical quality, overall > presentation, and information accuracy before passing out. I feel that > this should involve microscopic assessment, and also the judgment that > arrives from knowledge and experience to determine if the slide is > acceptable. I don't think it would be reasonable to expect all of this to > occur with a person who has not had the benefit of training. many errors > could be allowed out of the lab if labels are just put on without really > looking at the slides, just think of the impact on perception of quality, > accuracy and competency of histology. Most times, it is the "oops" that > seem to stay in memory, and not the bulk,? which go out fine. Not worth it > to me.Joelle > > http://www.linkedin.com/in/joelleweaver > >? > Date: Wed, 7 Dec _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Dec 7 16:47:18 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Wed Dec 7 16:47:25 2011 Subject: [Histonet] RE: Histology lab assistant Message-ID: This sounds like an ideal mix. Thanks for sharing-all people can help for sure. I appreciate that you expect people to strive for certification, still think that is important to the future field as a whole moving forward. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: D'Attilio Shelley Date: Wed, 7 Dec 2011 22:43:04 To: Subject: [Histonet] RE: Histology lab assistant Hi all, Matt asked about the duties of a non-certified Histology aide.? We have had this position for about 4 years and I consider it absolutely vital to the running of the lab.? We originally started with a half-time assistant working in the afternoons (the time with the busiest call volume).? This summer, I was able to increase the hours and make the position full time, working 0830-1700. The assistant's responsibilities are as follows: *?????? Filing slides and paperwork *?????? Morning slide distribution to pathologists (match slides to reports, last check of number of parts submitted) *?????? Answering the phone (a big boon to histotech productivity!) *?????? Coordinates send-out testing--pulls slides and block and takes to pathologist, fill out paperwork, package, etc. *?????? Moves slides from the stainer to the automatic coverslipper *?????? Some non-technical tasks related to our weekly Tumor board preparation *?????? Back-up gross room assistant for patient registration, specimen accessioning, and assist with gross exam *?????? Compilation of some CAP-required statistics (e.g. frozen section turnaround times) *?????? Reviewing bills and handling reference lab reports Eventually, this position may also be assigned minor daily maintenance on the slide labeler and H&E stainer and alcohol recycling. Giving these tasks to an assistant, especially answering the phone and managing sendouts, allows our histotechs to focus on their core competencies as well as other technical responsibilites such as regular procedure review and other items related to our CAP accreditation. Our professional staff is a mixture of HT(ASCP) and HTL(ASCP), with a few folks who are trained or in training.? These people have the necessary education to sit for the certification exam following their training period and they are expected to pass the exam as a condition of their continued employment. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From techonebs <@t> comcast.net Wed Dec 7 17:30:59 2011 From: techonebs <@t> comcast.net (Matt Mincer) Date: Wed Dec 7 17:31:04 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 11 Message-ID: <4EDFF733.406@comcast.net> Hey Eric, We make a universal, ergonomic microtome handle. You can find it at our site or from Newcomer Supply. http://www.newcomersupply.com/products/laboratory-equipment http://store.techonebiomedical.com/store/products/histo-comfort-grip.html Hope this helps. If you have any questions, feel free to call me. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 From techonebs <@t> comcast.net Wed Dec 7 17:31:45 2011 From: techonebs <@t> comcast.net (Matt Mincer) Date: Wed Dec 7 17:31:50 2011 Subject: [Histonet] Leica/Jung 2030 Microtome Ergonomic Handle Extension Message-ID: <4EDFF761.2010802@comcast.net> Hey Eric, We make a universal, ergonomic microtome handle. You can find it at our site or from Newcomer supply. http://www.newcomersupply.com/products/laboratory-equipment http://store.techonebiomedical.com/store/products/histo-comfort-grip.html Hope this helps. If you have any questions, feel free to call me. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 From Rcartun <@t> harthosp.org Wed Dec 7 17:59:51 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Dec 7 17:59:57 2011 Subject: [Histonet] IF on lung tissue Message-ID: <4EDFB7A8.7400.0077.1@harthosp.org> Does anyone still do direct immunofluorescence on frozen lung tissue looking for immune-complexes, and do you find it helpful clinically? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From ree3 <@t> leicester.ac.uk Thu Dec 8 04:47:20 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Dec 8 04:47:34 2011 Subject: [Histonet] antibody to eosinophil EG2 In-Reply-To: <4EDFB7A8.7400.0077.1@harthosp.org> References: <4EDFB7A8.7400.0077.1@harthosp.org> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4F1F9B6AA@EXC-MBX3.cfs.le.ac.uk> Looking for a supplier of the above antibody, I am aware that Pharmacia did supply it at one time, but contacting them to date has been fruitless, many thanks. Cheers Richard Edwards Leicester University U.K... From ree3 <@t> leicester.ac.uk Thu Dec 8 08:11:08 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Dec 8 08:11:25 2011 Subject: [Histonet] pharmacia In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101A4F1F9B6AA@EXC-MBX3.cfs.le.ac.uk> References: <4EDFB7A8.7400.0077.1@harthosp.org> <7722595275A4DD4FA225B92CDBF174A101A4F1F9B6AA@EXC-MBX3.cfs.le.ac.uk> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4F1F9B6AF@EXC-MBX3.cfs.le.ac.uk> And a related question, who is distributing Pharmacia's antibodies at the present?, many thanks. -----Original Message----- From: Edwards, Richard E. Sent: 08 December 2011 10:47 To: Histonet Subject: antibody to eosinophil EG2 Looking for a supplier of the above antibody, I am aware that Pharmacia did supply it at one time, but contacting them to date has been fruitless, many thanks. Cheers Richard Edwards Leicester University U.K... From dmlongoria <@t> ecrmc.org Thu Dec 8 08:17:18 2011 From: dmlongoria <@t> ecrmc.org (Diana Martinez-Longoria) Date: Thu Dec 8 08:17:32 2011 Subject: [Histonet] ASCP Membership Message-ID: <12B71261212BE94BB9FB7735484B9FA11AF011@EXMBX01.ecrmc.ci.el-centro.ca.us> Hello, Thank you to all that responded to my question regarding ASCP membership, now I can make my decision. Have a great Merry Christmas! Diana Martinez-Longoria Histotechnician (ASCP)cm El Centro Regional Medical Center phone: 760-339-7267 fax: 760-482-5365 email:dmlongoria@ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From Ronald.Houston <@t> nationwidechildrens.org Thu Dec 8 08:22:47 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Dec 8 08:22:56 2011 Subject: [Histonet] EM fixative composition Message-ID: Trying to get a sense of how people are making up their glutaradelhyde for EM on clinical specimens. Is anyone buying it in premade, and if so from whom? What buffer system are you using? phosphate or cacodylate (or something else)? Are you checking the osmolality of the fixative before use? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From idimitro <@t> mun.ca Thu Dec 8 08:39:03 2011 From: idimitro <@t> mun.ca (idimitro@mun.ca) Date: Thu Dec 8 08:39:08 2011 Subject: [Histonet] RE: EM fixative composition In-Reply-To: References: Message-ID: <14C3108E8B98EF43B3EDAE583367003405C870@exchange.med.mun.ca> Hi Ronnie, For EM on clinical samples we use Karnovsky's fixative, which is a glutaraldehyde/paraformaldehyde solution in 0.1M cacodylate buffer. We prepare our own but you can buy it. We found it does the best job in preserving the cell organelles and membranes. We do not check the osmolality, but we check the pH and adjust accordingly to 7.4. Keep in the fridge. I hope this helps. Iliana Dimitrova, B. Tech, MSc, RT Histology Supervisor Room 2808 Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 Tel: 709.777.8389 Fax: 709.777.6746 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: December 8, 2011 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EM fixative composition Trying to get a sense of how people are making up their glutaradelhyde for EM on clinical specimens. Is anyone buying it in premade, and if so from whom? What buffer system are you using? phosphate or cacodylate (or something else)? Are you checking the osmolality of the fixative before use? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php From sfonner <@t> labpath.com Thu Dec 8 09:18:03 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Thu Dec 8 09:23:27 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <1323298038.71111.YahooMailNeo@web112310.mail.gq1.yahoo.com> References: <1937.208.62.167.196.1323290758.squirrel@webmail.realpages.com> <1323294439.8349.YahooMailClassic@web65715.mail.ac4.yahoo.com> <1323298038.71111.YahooMailNeo@web112310.mail.gq1.yahoo.com> Message-ID: <000001ccb5bc$943e9430$bcbbbc90$@com> WELL SAID!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Wednesday, December 07, 2011 5:47 PM To: Rene J Buesa; joelle weaver; histonet@lists.utsouthwestern.edu; Nicole Tatum Subject: Re: [Histonet] Histo Aide Duties I'm sure there are worse comments that could have been made to your "opinion" that someone should be licensed to coverslip. Obviously you realize the cost for labs for such an act? Coverslipping is not technical in my opinion. (or CLIA's and CAP's ) ? Yes, supervisors are mandated with the responsibility of making sure the product are of high quality. But they have regulations and guidelines they follow. Back to the CLIA/CAP guidelines. ? Quality of slides should be monitored with daily QC. Ive often done this by filling out a QC sheet with a certain % of cases to have a Pathologist sign off on. CAP loves this type of thing. ? It doesnt provide any of us any service to our profession to belittle those who help us, those aides. The aides of which I have had many who hold BS degrees in science feilds who do not have a certification or licensure as a Histotech. Many of whom could whip out a FNA cytospin with perfection all at the same time coverslipping a hundred more. ? Rene, I just think you shocked some of us with your opinion that coverslipping should be done by licensed personel. ? I hope you dont take any of this as personal. This subject should have just remained per the laws/regulations as it was ask. ? Things always get messy when we put our opinions in. ? Now I've put mine in and I'm running for the hills......................................... ? KD ________________________________ From: Rene J Buesa To: joelle weaver ; histonet@lists.utsouthwestern.edu; Nicole Tatum Sent: Wednesday, December 7, 2011 4:47 PM Subject: RE: [Histonet] Histo Aide Duties I think that using the qualifier "silly" is not called for. Regardless of how things were done years ago it does not mean that they ought to continue that way. As the last step I personally think (even if you think it is "silly") that?cover-slipping should NOT be completed by an unlicensed person with not enough knowledge for the task. An aide is just that, an aide that can be very helpful in completing some tasks efficiently and cheaper than a HT, but that ought not to be allowed to do technical tasks. As to automated cover-slippers go?the final product should be inspected by a licensed HT before sending it to the pathologists. It is just a way of increasing productivity and cutting costs. Regardless of all, a lab is run by a supervisor and she or he is the one who decides what tasks can be done by an aide, even if he or she ends as being considered "silly". Ren? J. --- On Wed, 12/7/11, Nicole Tatum wrote: From: Nicole Tatum Subject: RE: [Histonet] Histo Aide Duties To: "joelle weaver" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 3:45 PM Most professional labs require a person who has completed an accrediated histology programs, but CLIA states that a person can work within a histology lab if they have a combination of 12hrs of college chemistry or biology. In any combination. Then that person needs to be trained in each area they will work in and be signed off on by the directions as being able to perform tasks. To say that an unlicensed person working in histology can not coverslip is silly. I agree that this is the last chance to verify stain quality as well as tissue quality, but, before licensure there were thousands of OJT employees filling our histology positions. Many of whom trained each of us to have a critical eye when we were students performing our internships. If the person is working in ur labs as an unlicensed assistant, you must of had some confidence in them or you would not have them working in your lab.? The evolution and technical skill of our trade has been taught from one person to another, at a time when licensure was not as important. That being said, licensure is now becomming critial to work in our field. But that does not mean that currecntly there are many unlicensed competent persons working in histology. They work as Mohs techs , and prep techs, and gross techs, etc. I would hope that a nonlicensed Mohs tech would know how to access their slides and coverslip effectively. My point, all persons working in our field contribute and get the job done, despite their licensure situations. They could still be very competent individuals if training by a patient person who is willing to educate others. Nicole Tatum HT ASCP ASMS > I don't want to seem nit picky, but I tend to strongly agree with > Rene's point about coverslipping. It is not really the act or task of > coverslipping to me, but rather the fact that it is one of the last > opportunities to assess the slide for technical quality, overall > presentation, and information accuracy before passing out. I feel that > this should involve microscopic assessment, and also the judgment that > arrives from knowledge and experience to determine if the slide is > acceptable. I don't think it would be reasonable to expect all of this > to occur with a person who has not had the benefit of training. many > errors could be allowed out of the lab if labels are just put on > without really looking at the slides, just think of the impact on > perception of quality, accuracy and competency of histology. Most > times, it is the "oops" that seem to stay in memory, and not the bulk,? > which go out fine. Not worth it to me.Joelle > > http://www.linkedin.com/in/joelleweaver > >? > Date: Wed, 7 Dec _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Dec 8 09:52:25 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Dec 8 09:52:26 2011 Subject: [Histonet] RE: EM fixative composition In-Reply-To: <14C3108E8B98EF43B3EDAE583367003405C870@exchange.med.mun.ca> References: <14C3108E8B98EF43B3EDAE583367003405C870@exchange.med.mun.ca> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5C28@PHSXMB30.partners.org> We are a research lab, but we use K2 (Karnovsky's fix) as well. Since paraformaldehyde is hazardous (and a bit involved to make up), we simply buy the glutaraldehyde and paraformaldehyde in sealed ampules and make it up in a 0.1M cacodylate buffer. We also check the pH (7.4), not the osmolality. I freeze aliquots (3ml) and then thaw to use. Works out well. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of idimitro@mun.ca Sent: Thursday, December 08, 2011 9:39 AM To: Ronald.Houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: EM fixative composition Hi Ronnie, For EM on clinical samples we use Karnovsky's fixative, which is a glutaraldehyde/paraformaldehyde solution in 0.1M cacodylate buffer. We prepare our own but you can buy it. We found it does the best job in preserving the cell organelles and membranes. We do not check the osmolality, but we check the pH and adjust accordingly to 7.4. Keep in the fridge. I hope this helps. Iliana Dimitrova, B. Tech, MSc, RT Histology Supervisor Room 2808 Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 Tel: 709.777.8389 Fax: 709.777.6746 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: December 8, 2011 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EM fixative composition Trying to get a sense of how people are making up their glutaradelhyde for EM on clinical specimens. Is anyone buying it in premade, and if so from whom? What buffer system are you using? phosphate or cacodylate (or something else)? Are you checking the osmolality of the fixative before use? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From hhawkins <@t> UTMB.EDU Thu Dec 8 10:34:58 2011 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Thu Dec 8 10:35:02 2011 Subject: [Histonet] RE: EM fixative composition In-Reply-To: <14C3108E8B98EF43B3EDAE583367003405C870@exchange.med.mun.ca> References: , <14C3108E8B98EF43B3EDAE583367003405C870@exchange.med.mun.ca> Message-ID: <22624908330375439D6382C9F95093FF68CB46@GRMBX1.utmb.edu> McDowell-Trump fixative is an inexpensive formulation of glutaraldehyde and formaldehyde and is available ready-made from EMS Diasum. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of idimitro@mun.ca [idimitro@mun.ca] Sent: Thursday, December 08, 2011 8:39 AM To: Ronald.Houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: EM fixative composition Hi Ronnie, For EM on clinical samples we use Karnovsky's fixative, which is a glutaraldehyde/paraformaldehyde solution in 0.1M cacodylate buffer. We prepare our own but you can buy it. We found it does the best job in preserving the cell organelles and membranes. We do not check the osmolality, but we check the pH and adjust accordingly to 7.4. Keep in the fridge. I hope this helps. Iliana Dimitrova, B. Tech, MSc, RT Histology Supervisor Room 2808 Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 Tel: 709.777.8389 Fax: 709.777.6746 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: December 8, 2011 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EM fixative composition Trying to get a sense of how people are making up their glutaradelhyde for EM on clinical specimens. Is anyone buying it in premade, and if so from whom? What buffer system are you using? phosphate or cacodylate (or something else)? Are you checking the osmolality of the fixative before use? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Dec 8 11:44:06 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Dec 8 11:44:27 2011 Subject: [Histonet] RE: lab chairs In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC0656D5@JERRY.Gia.com> References: <8287D830EC5C904AA3E482C75773A03B0A0ECBBB@EX1.YPIILab.YPII.com> <5A33C952BB67F4468AF1F36D739212BC0656D5@JERRY.Gia.com> Message-ID: We use backless drafting stools made by Office Star ordered from www.cymax.com. The saddle seat gets funny looks but we wouldn't use anything else now - they roll, tilt, go up and down and eliminate the pressure on the thighs from the front edge of a regular chair. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, December 07, 2011 10:48 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] lab chairs Where do you recommend buying lab chairs? I'm looking for some w/o arm rests, solid black or tan, on rollers, etc.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson <@t> primecare.org Thu Dec 8 11:56:54 2011 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Thu Dec 8 11:57:03 2011 Subject: [Histonet] RE: lab chairs In-Reply-To: References: <8287D830EC5C904AA3E482C75773A03B0A0ECBBB@EX1.YPIILab.YPII.com> <5A33C952BB67F4468AF1F36D739212BC0656D5@JERRY.Gia.com> Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C8FF2@EXCHANGE2K7.staprimecare.org> Amber, We recently purchased chairs from VWR International LLC and are pleased with them. They are solid black, no arm rests, have adjustments for ergonomic settings, on rollers, etc.... The catalog numbers are 80086-438 and 80086-440 if you want to look them up on their website. The price was reasonable too. Others on the histonet had recommended these to me as they sounded durable in quality. We have multiple staff interchange on the benches throughout the day, so the ability to adjust for each one's body type was important to us. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Thursday, December 08, 2011 11:44 AM To: Amber McKenzie; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: lab chairs We use backless drafting stools made by Office Star ordered from www.cymax.com. The saddle seat gets funny looks but we wouldn't use anything else now - they roll, tilt, go up and down and eliminate the pressure on the thighs from the front edge of a regular chair. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, December 07, 2011 10:48 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] lab chairs Where do you recommend buying lab chairs? I'm looking for some w/o arm rests, solid black or tan, on rollers, etc.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From abilger <@t> wellspan.org Thu Dec 8 12:16:44 2011 From: abilger <@t> wellspan.org (Bilger, Andrea) Date: Thu Dec 8 12:17:22 2011 Subject: [Histonet] Tissue processors Message-ID: <6D7752544B308D44A902C0BD0EC7BF5C8C5991C9@EXCH02.wellspan.org> Histonetters, Do any of you have any experience with the Lieca Peloris II tissue processor? Andrea Bilger Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From brannon <@t> alliedsearchpartners.com Thu Dec 8 13:47:11 2011 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Dec 8 13:47:27 2011 Subject: [Histonet] Histology (MOHS) Technician Needed in Georgetown, TX Message-ID: Allied Search Partners is searching for a qualified MOHS Technician (Histology) for a laboratory in Central Texas. Location: Georgetown, TX. Just North of Austin, TX. Shift: Full Time. Monday-Friday. This is a permanent/long term position. Duties & Responsibilities: ? Assist MOHS Surgeon in a High Volume Laboratory ? Embedding, Cutting, Staining, Coverslipping, Presenting Slides, Coordinating Surgery Maps, Filing Slides, and Paperwork. ? Prepping for Cases. ? Ordering Slides. ? Performing general lab duties and related competencies for MOHS surgical service. To Apply: Please send resume to Brannon@alliedsearchpartners.com . At that time, one of our recruiters will contact you if all qualifications are met. Thank you! -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From rsrichmond <@t> gmail.com Thu Dec 8 14:19:28 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Dec 8 14:19:33 2011 Subject: [Histonet] pathology grossing stations Message-ID: A laboratory I'm doing some work for is planning to remodel within two months. We see about 5,000 specimens a year, with a moderate number of hospital major specimens including placentas. Currently we have no grossing station, and this may be an opportunity to get one. It will be installed in a windowless unventilated room, like our present sink setup. I don't know the dimensions of the space available. I suspect the decision will be made on the basis of price, with us having very little input. What currently available grossing station do you have? Do you like it? Bob Richmond Samurai Pathologist Knoxville TN From trathborne <@t> somerset-healthcare.com Thu Dec 8 14:43:45 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Dec 8 14:43:57 2011 Subject: [Histonet] pathology grossing stations In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F6536E@SMCMAIL01.somerset-healthcare.com> Check the Mopec website ( www.mopec.com ). They have a nice selection of stand-alone and counter-top models that use filters and do not have to be vented to the outside. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, December 08, 2011 3:19 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] pathology grossing stations A laboratory I'm doing some work for is planning to remodel within two months. We see about 5,000 specimens a year, with a moderate number of hospital major specimens including placentas. Currently we have no grossing station, and this may be an opportunity to get one. It will be installed in a windowless unventilated room, like our present sink setup. I don't know the dimensions of the space available. I suspect the decision will be made on the basis of price, with us having very little input. What currently available grossing station do you have? Do you like it? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From brannon <@t> alliedsearchpartners.com Thu Dec 8 15:59:13 2011 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Dec 8 15:59:45 2011 Subject: [Histonet] MOHS Histotech Needed in Dover, DE Message-ID: Allied Search Partners is looking for a qualified professional to fill the below position. Position Title: MOHS Histotech Schedule: Full Time, Monday-Friday, Day Shift. This is a permanent/long term position. Location: Dover, DE Summary: ? Perform Histology and Cytology preparation procedures on a patient specimens submitted for the diagnoses of disease. ? Maintain specimen identity and integrity; produces high quality stained slides using standardized procedures to meet the Pathologists requirements for microscopic evaluation; performs quality control and preventive maintenance according to established policy. ? Works independently organizing work to meet established policy and deadlines. ? Interact with patient populations from neonate to elderly; with patients of al types of illnesses ? Process, Embed, Cut and Stain quality frozen tissue specimens within established time limits ? Works with the MOHS Dermatologist to resolve quality control, patient, or procedural problems. Duties: ? Process, embed, cut and stain quality frozen tissue specimens within established time limits ? Work with the MOHS Dermatologist to resolve quality control, patient, or procedural problems. To find out more: Please submit resume to Brannon@alliedsearchpartners.com and one of our recruiters will contact you for a phone screen if all requirements are met. Thank you! -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From dmburns9 <@t> gmail.com Thu Dec 8 16:29:31 2011 From: dmburns9 <@t> gmail.com (Douglas M Burns) Date: Thu Dec 8 16:29:34 2011 Subject: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain Message-ID: Hello, Histo-users, Follow-up to our original posting about fixed-brain cryosections falling off of subbed slides. We fished out our Cryo-Jane apparatus, and using this apparatus has resulted in a great improvement in sections and getting sections stuck onto the slides. This works pretty well until we start using the ethanol series to dehydrate and delipidate before a Crystal violet stain for Nissl bodies. (That is, for acqueous steps everything seems to stay glued to the slide; however, once ethanols are involved pieces start to come off.) To summarize, we are trying to go from cut blocks of paraformaldehyde-fixed brain infused with sucrose and then frozen at -80. We take them out, unfrozen, and cut them on a Leica cryostat. Early attempts with subbed slides did not work reliably, so we have turned to 'Jane. Jane is slightly slower than a brush, and seems to work fairly well. However, we have had trouble with the tape pulling parts of sections off of the glued slides. When we introduce the nonacqeous solvents, our problems become more obvious. We have not so far gone to a post-fix, because we wanted to simplify the overall process. Could this be the mistake? We want to use IHC for c-Fox and possibly orexin - and similar - but we want cresyl-stain to locate positively the area of interest in the cut block. Any suggestions? Also, are there any 'Jane enthusiasts out there? thank you in advance --------------- Doug Burns, Kansas City From jnocito <@t> satx.rr.com Thu Dec 8 17:05:15 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Dec 8 17:06:19 2011 Subject: [Histonet] pathology grossing stations In-Reply-To: References: Message-ID: <3170EB57581D499989E2B5DB33B4E17F@JoePC> Bob, try Mopec. When I was at this year's NSH in Cinci, they had a few different sizes and configurations. Joe the Toe ----- Original Message ----- From: "Bob Richmond" To: "Histonet@lists.utsouthwestern.edu" Sent: Thursday, December 08, 2011 2:19 PM Subject: [Histonet] pathology grossing stations >A laboratory I'm doing some work for is planning to remodel within two > months. We see about 5,000 specimens a year, with a moderate number of > hospital major specimens including placentas. Currently we have no > grossing station, and this may be an opportunity to get one. It will > be installed in a windowless unventilated room, like our present sink > setup. I don't know the dimensions of the space available. I suspect > the decision will be made on the basis of price, with us having very > little input. > > What currently available grossing station do you have? Do you like it? > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Dec 8 20:46:58 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Dec 8 20:47:03 2011 Subject: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain In-Reply-To: References: Message-ID: Doug, Hopefully you are using the CryoJane slides (not the subbed slides) and the UV glue activator and keeping both slides and tape cold in the cryostat. Be aware that you can get CryoJane slides with different amounts of adhesive (0.5-4x). I would suggest going to the 4x slides. When peeling the tape off I pull it slowly and evenly almost back on itself at an acute angle- this greatly reduces the incidence of the section coming off with the tape... Haven't had a problem with section detachment in ethanols. Brett Brett Connolly, Phd Merck Research Labs West Point, PA ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns [dmburns9@gmail.com] Sent: Thursday, December 08, 2011 5:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain Hello, Histo-users, Follow-up to our original posting about fixed-brain cryosections falling off of subbed slides. We fished out our Cryo-Jane apparatus, and using this apparatus has resulted in a great improvement in sections and getting sections stuck onto the slides. This works pretty well until we start using the ethanol series to dehydrate and delipidate before a Crystal violet stain for Nissl bodies. (That is, for acqueous steps everything seems to stay glued to the slide; however, once ethanols are involved pieces start to come off.) To summarize, we are trying to go from cut blocks of paraformaldehyde-fixed brain infused with sucrose and then frozen at -80. We take them out, unfrozen, and cut them on a Leica cryostat. Early attempts with subbed slides did not work reliably, so we have turned to 'Jane. Jane is slightly slower than a brush, and seems to work fairly well. However, we have had trouble with the tape pulling parts of sections off of the glued slides. When we introduce the nonacqeous solvents, our problems become more obvious. We have not so far gone to a post-fix, because we wanted to simplify the overall process. Could this be the mistake? We want to use IHC for c-Fox and possibly orexin - and similar - but we want cresyl-stain to locate positively the area of interest in the cut block. Any suggestions? Also, are there any 'Jane enthusiasts out there? thank you in advance --------------- Doug Burns, Kansas City _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From joost.bruijntjes <@t> tno.triskelion.nl Fri Dec 9 04:47:47 2011 From: joost.bruijntjes <@t> tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Fri Dec 9 04:47:55 2011 Subject: [Histonet] Toluidine blue Message-ID: Henk Al weer even geleden: weet je al iets over de toepassing van CD4 op varkensweefsel formaline gefixeerd en in paraffine ingebed weefsel? Heb nog een vraag: zijn jullie in het bezit van een cryostaat waar je nog met ouderwetse vaste messen kan snijden? Ik ben op zoek naar de mogelijkheid om m.b.v. een D-mes bothoudend/verkalkte weefsels te snijden. We hebben hier een microm, maar die is zoals die hier nu staat alleen toepasbaar voor disposable mesjes. Een aanpassing om vaste messen te gaan gebruiken kost een ruime 1000 euro en als het niet zou werken is dat zonde-geld. Alvast bedankt Joost TNO.NL Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijntjes@tno.triskelion.nl Disclaimer From JoelI <@t> mcclainlab.com Fri Dec 9 06:03:31 2011 From: JoelI <@t> mcclainlab.com (Joel Israel) Date: Fri Dec 9 06:03:29 2011 Subject: [Histonet] Propidium Iodide Message-ID: <2FFA57F6EC24E14493FAC3DE629AD5B01484DB@ML1.McClainLabs.local> Has anyone ever used Fluoroshield Mounting Medium with Propidium Iodide by Abcam or anything similar? How were the results? Thank you in advance. ~Joel From flnails <@t> texaschildrens.org Fri Dec 9 08:20:12 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Dec 9 08:20:26 2011 Subject: [Histonet] pathology grossing stations In-Reply-To: <3170EB57581D499989E2B5DB33B4E17F@JoePC> References: <3170EB57581D499989E2B5DB33B4E17F@JoePC> Message-ID: I agree with Joe, Mopec makes a very good product. In the last 4 years I have purchased 6 MB6000 and our pathologist are very pleased with them, especially the absence of high noise levels and adjustability. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, December 08, 2011 5:05 PM To: Bob Richmond; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] pathology grossing stations Bob, try Mopec. When I was at this year's NSH in Cinci, they had a few different sizes and configurations. Joe the Toe ----- Original Message ----- From: "Bob Richmond" To: "Histonet@lists.utsouthwestern.edu" Sent: Thursday, December 08, 2011 2:19 PM Subject: [Histonet] pathology grossing stations >A laboratory I'm doing some work for is planning to remodel within two > months. We see about 5,000 specimens a year, with a moderate number of > hospital major specimens including placentas. Currently we have no > grossing station, and this may be an opportunity to get one. It will > be installed in a windowless unventilated room, like our present sink > setup. I don't know the dimensions of the space available. I suspect > the decision will be made on the basis of price, with us having very > little input. > > What currently available grossing station do you have? Do you like it? > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From CIngles <@t> uwhealth.org Fri Dec 9 08:47:34 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Fri Dec 9 08:48:17 2011 Subject: [Histonet] Toluidine blue References: Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A7A3@UWHC-MAIL2.uwhis.hosp.wisc.edu> Anyone know Dutch? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bruijntjes, J.P. (Joost) Sent: Fri 12/9/2011 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Henk Al weer even geleden: weet je al iets over de toepassing van CD4 op varkensweefsel formaline gefixeerd en in paraffine ingebed weefsel? Heb nog een vraag: zijn jullie in het bezit van een cryostaat waar je nog met ouderwetse vaste messen kan snijden? Ik ben op zoek naar de mogelijkheid om m.b.v. een D-mes bothoudend/verkalkte weefsels te snijden. We hebben hier een microm, maar die is zoals die hier nu staat alleen toepasbaar voor disposable mesjes. Een aanpassing om vaste messen te gaan gebruiken kost een ruime 1000 euro en als het niet zou werken is dat zonde-geld. Alvast bedankt Joost TNO.NL Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijntjes@tno.triskelion.nl Disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Dec 9 08:49:53 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Fri Dec 9 08:50:15 2011 Subject: [Histonet] Toluidine blue In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A7A3@UWHC-MAIL2.uwhis.hosp.wisc.edu> References: <064F1ACBAE8A78469AE2E41D533D87E505A7A3@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4F1F9B6D8@EXC-MBX3.cfs.le.ac.uk> Yes, I met him in a bar in old Soho.......... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 09 December 2011 14:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Toluidine blue Anyone know Dutch? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bruijntjes, J.P. (Joost) Sent: Fri 12/9/2011 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Henk Al weer even geleden: weet je al iets over de toepassing van CD4 op varkensweefsel formaline gefixeerd en in paraffine ingebed weefsel? Heb nog een vraag: zijn jullie in het bezit van een cryostaat waar je nog met ouderwetse vaste messen kan snijden? Ik ben op zoek naar de mogelijkheid om m.b.v. een D-mes bothoudend/verkalkte weefsels te snijden. We hebben hier een microm, maar die is zoals die hier nu staat alleen toepasbaar voor disposable mesjes. Een aanpassing om vaste messen te gaan gebruiken kost een ruime 1000 euro en als het niet zou werken is dat zonde-geld. Alvast bedankt Joost TNO.NL Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijntjes@tno.triskelion.nl Disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bboyce <@t> NEMOURS.ORG Fri Dec 9 09:04:24 2011 From: bboyce <@t> NEMOURS.ORG (Boyce, Bobbie) Date: Fri Dec 9 09:04:35 2011 Subject: [Histonet] Toluidine blue In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A7A3@UWHC-MAIL2.uwhis.hosp.wisc.edu> References: <064F1ACBAE8A78469AE2E41D533D87E505A7A3@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: I put it in a translator program and it's something like this: long time ago: you know anything about the application of cd4 on varkensweefsel formalin fixed and in paraffin embedded fabric? have another question: are you in the possession of a cryostat where you still with old-fashioned fixed knives, cutting up? I am trying to the possibility of using a D-Knife bothoudend/i fabrics to cut off. We have here a microm, but which is as in here and now only applicable for consumer blades. Babylon 9 Bobbie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, December 09, 2011 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Toluidine blue Anyone know Dutch? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bruijntjes, J.P. (Joost) Sent: Fri 12/9/2011 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Henk Al weer even geleden: weet je al iets over de toepassing van CD4 op varkensweefsel formaline gefixeerd en in paraffine ingebed weefsel? Heb nog een vraag: zijn jullie in het bezit van een cryostaat waar je nog met ouderwetse vaste messen kan snijden? Ik ben op zoek naar de mogelijkheid om m.b.v. een D-mes bothoudend/verkalkte weefsels te snijden. We hebben hier een microm, maar die is zoals die hier nu staat alleen toepasbaar voor disposable mesjes. Een aanpassing om vaste messen te gaan gebruiken kost een ruime 1000 euro en als het niet zou werken is dat zonde-geld. Alvast bedankt Joost TNO.NL Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijntjes@tno.triskelion.nl Disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smcbride <@t> andrew.cmu.edu Fri Dec 9 10:59:05 2011 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Fri Dec 9 10:59:13 2011 Subject: [Histonet] Need SOP for Sanderson's for Thin Section Message-ID: <8AB969BF-378A-466C-B492-5E994BFE5BF3@andrew.cmu.edu> Hi folks, I have used Sanderson's stain for my thick, ground bone specimens successfully for years, but have never tried it with thin section specimens. I have a thin section study that I need to stain with Sanderson's, but am unsure of staining times and temperatures for these de-plasticized slides. Does anyone have a thin section SOP that they would be willing to share? Thanks! ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu From jpgrock37 <@t> yahoo.com Fri Dec 9 11:25:45 2011 From: jpgrock37 <@t> yahoo.com (Jessica Piche) Date: Fri Dec 9 11:25:51 2011 Subject: [Histonet] Marking pens coming off cassettes Message-ID: <1323451545.54880.YahooMailNeo@web121519.mail.ne1.yahoo.com> Hey All! We have been having problems with our marking pens (Secureline/Superfrost). We have two processors, an old VIP and a Thermo Excelsior. The pens seems to smudge on both machines, but especially on the VIP. We tried some sample pens from other companies and they too have smudged. Any ideas whats going on? Thanks, ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From sdysart <@t> mirnarx.com Fri Dec 9 11:32:33 2011 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Fri Dec 9 11:32:48 2011 Subject: [Histonet] Filter Paper Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D50022156@SN2PRD0702MB110.namprd07.prod.outlook.com> Hey guys, What is the standard filter paper for hematoxylin. I threw away the box so now I don't know the grade...oops Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From rjbuesa <@t> yahoo.com Fri Dec 9 11:34:57 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 9 11:35:04 2011 Subject: [Histonet] Marking pens coming off cassettes In-Reply-To: <1323451545.54880.YahooMailNeo@web121519.mail.ne1.yahoo.com> Message-ID: <1323452097.71035.YahooMailClassic@web65715.mail.ac4.yahoo.com> It could be the pens or something in the cassettes. Check if something about the cassettes or how they are kept has changed. Any possibility of any type of oily solution mixed with the NBF? Ren? J. --- On Fri, 12/9/11, Jessica Piche wrote: From: Jessica Piche Subject: [Histonet] Marking pens coming off cassettes To: "Histonet@lists.utsouthwestern.edu" Date: Friday, December 9, 2011, 12:25 PM Hey All! We have been having problems with our marking pens (Secureline/Superfrost). We have two processors, an old VIP and a Thermo Excelsior. The pens seems to smudge on both machines, but especially on the VIP. We tried some sample pens from other companies and they too have smudged. Any ideas whats going on? Thanks, ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Dec 9 11:38:23 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Dec 9 11:37:53 2011 Subject: [Histonet] Marking pens coming off cassettes In-Reply-To: <1323451545.54880.YahooMailNeo@web121519.mail.ne1.yahoo.com> References: <1323451545.54880.YahooMailNeo@web121519.mail.ne1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA01F4D@IBMB7Exchange.digestivespecialists.com> You might want to check your cassettes. We experienced the same problem and it turned out to be the surface of the cassette was causing the problem. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, December 09, 2011 12:26 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking pens coming off cassettes Hey All! We have been having problems with our marking pens (Secureline/Superfrost). We have two processors, an old VIP and a Thermo Excelsior. The pens seems to smudge on both machines, but especially on the VIP. We tried some sample pens from other companies and they too have smudged. Any ideas whats going on? Thanks, ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chak_bou <@t> yahoo.com Fri Dec 9 13:29:30 2011 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Fri Dec 9 13:29:32 2011 Subject: [Histonet] Necropsy camera Message-ID: <1323458970.53613.YahooMailClassic@web161804.mail.bf1.yahoo.com> Hi All, I am trying to buy a camera to our necropsy lab does anyone has any suggestion where can I find a suitable one? thank you in advance for your help. appreciated Chakib Boussahmain Histology/Necropsy supervisor MIT- DCM From wilson6848 <@t> yahoo.com Sun Dec 11 22:27:46 2011 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Sun Dec 11 22:27:50 2011 Subject: [Histonet] Looking for Position in IHC/HISTOLOGY In-Reply-To: <1321922640.8772.YahooMailNeo@web120913.mail.ne1.yahoo.com> References: <1309821326.26450.YahooMailRC@web120902.mail.ne1.yahoo.com> <1321922640.8772.YahooMailNeo@web120913.mail.ne1.yahoo.com> Message-ID: <1323664066.29729.YahooMailNeo@web120916.mail.ne1.yahoo.com> ?? ?I am an HTL(ASCP)QIHC Certified Histotech with an extensive experience in IHC. I am looking for position in IHC/HISTOLOGY.? Willing to relocate. ?Thanks,?Wilson. From kmerriam2003 <@t> yahoo.com Mon Dec 12 07:09:36 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Dec 12 07:09:41 2011 Subject: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain In-Reply-To: References: Message-ID: <1323695376.13216.YahooMailNeo@web130123.mail.mud.yahoo.com> I was having similar issues with frozen rat and human cartilage with the cryojane.? After a lot of trial and error; I have found that the 4X slides and air-drying overnight has worked extremely well for us.? The tissues never fall off any more! ? Good luck, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Connolly, Brett M" To: Douglas M Burns ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, December 8, 2011 9:46 PM Subject: RE: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain Doug, Hopefully you are using the CryoJane slides (not the subbed slides) and the UV glue activator and keeping both slides and tape cold in the cryostat. Be aware that you can get CryoJane slides with different amounts of adhesive (0.5-4x).? I would suggest going to the 4x slides. When peeling the tape off I pull it slowly and evenly almost back on itself at an acute angle- this greatly reduces the incidence of the section coming off with the tape... Haven't had a problem with section detachment in ethanols. Brett Brett Connolly, Phd Merck Research Labs West Point, PA ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns [dmburns9@gmail.com] Sent: Thursday, December 08, 2011 5:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain Hello, Histo-users, ? ? Follow-up to our original posting about fixed-brain cryosections falling off of subbed slides. We fished out our Cryo-Jane apparatus, and using this apparatus has resulted in a great improvement in sections and getting sections stuck onto the slides. This works pretty well until we start using the ethanol series to dehydrate and delipidate before a Crystal violet stain for Nissl bodies. (That is, for acqueous steps everything seems to stay glued to the slide; however, once ethanols are involved pieces start to come off.) ? ? ? To summarize, we are trying to go from cut blocks of paraformaldehyde-fixed brain infused with sucrose and then frozen at -80. We take them out, unfrozen, and cut them on a Leica cryostat. Early attempts with subbed slides did not work reliably, so we have turned to 'Jane. Jane is slightly slower than a brush, and seems to work fairly well. However, we have had trouble with the tape pulling parts of sections off of the glued slides. When we introduce the nonacqeous solvents, our problems become more obvious. ? ? ? We? have not so far gone to a post-fix, because we wanted to simplify the overall process. Could? this be the mistake? ? ? ? We want to use IHC for c-Fox and possibly orexin - and similar - but we want cresyl-stain to locate positively the area of interest in the cut block. ? ? ? Any suggestions?? Also, are there any 'Jane enthusiasts out there? ? ? ? ? ? ? ? ? ? ? ? ? ? ? thank? you in advance? ---------------? Doug Burns, Kansas City _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pbourassa <@t> karospharma.com Mon Dec 12 08:29:46 2011 From: pbourassa <@t> karospharma.com (Patricia Bourassa) Date: Mon Dec 12 08:29:54 2011 Subject: [Histonet] Tissue Processor Message-ID: Hello! We are looking to purchase a used tissue processor for a small biotech. We will be working with calcified bone so we will only use the machine for dehydration and will infiltrate with methacrylate by hand. I'm interested in suggestions. It's been a while since I've done histology and I'd like to know what machines/ vendors folks would recommend. Thanks for your help! Patti -- *Patti Bourassa Senior Scientist Karos Pharmaceuticals (203) 535-0540, ext 207* From pbourassa <@t> karospharma.com Mon Dec 12 08:32:06 2011 From: pbourassa <@t> karospharma.com (Patricia Bourassa) Date: Mon Dec 12 08:32:13 2011 Subject: [Histonet] Gayle Callis Message-ID: I'm trying to contact Gayle... is she still on the histonet? -- *Patti Bourassa Senior Scientist Karos Pharmaceuticals (203) 535-0540, ext 207* From mward <@t> wakehealth.edu Mon Dec 12 10:53:31 2011 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Mon Dec 12 10:53:46 2011 Subject: [Histonet] PAX 2 antibody Message-ID: Hello all, I am still hunting for a source for the PAX 2 antibody. Is this antibody currently available? I have heard in the past that there was a manufacturing issue. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From melissa <@t> alliedsearchpartners.com Mon Dec 12 11:04:14 2011 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Mon Dec 12 11:04:27 2011 Subject: [Histonet] Histology Supervisor Job Opening in Naples, FL Message-ID: Allied Search Partners is conducting a search for a qualified Histology Supervisor. This candidate must be Florida Licensed as a Supervisor and have a Bachelor?s Degree or higher to qualify for this position. Please let me know if you, or anyone that you know would be a great fit for this job. Position: Histology Supervisor Shift: 8am-4:30pm Full time/Permanent Location: Naples, FL To Apply: Please send current resume as well as salary expectations to Melissa@alliedsearchpartners.com for review. Qualified applicants will be contacted by one of our recruiters for a phone prescreen. Position Summary: Allied Search Partners is looking for a qualified Histology Supervisor for a state-of-the-art laboratory in Naples, FL. ? Provides direct supervision to the Histology section coordinating workflow and overseeing the scheduling of staff. ? Laboratory Supervisor maintains instruments, monitors proficiency testing and quality control, actively participates in performance improvement, and develops new procedures. ? Facilitates employee training and competence assessment. ? Maintains supplies and inventory in a cost effective manner. ? Develops laboratory personnel through continuing education opportunities and delegates special projects where appropriate. ? Complies with and enforces policies and procedures in a consistent and timely manner with a fair consideration for the associate. ? Assures minimal downtime of instruments by planning for preventative maintenance and contacting service personnel when unavoidable repairs are necessary. ? Assesses costs of supplies and reviews inventory levels and usage rates to ensure adequate supplies are stocked to meet workload demands. Requirements: ? BA/BS in related field required ? Current Florida licensure as a Histology Supervisor ? 3+ years clinical laboratory experience -- Melissa Phelan, President Laboratory Staffing Allied Search Partners http://www.linkedin.com/in/melissaphelan P: 888-388-7571 F: 888-388-7572 C: 407-697-1175 www.alliedsearchpartners.com From Dana.Spencer <@t> PCMH.COM Mon Dec 12 13:25:39 2011 From: Dana.Spencer <@t> PCMH.COM (Dana Spencer) Date: Mon Dec 12 13:26:09 2011 Subject: [Histonet] Morgue Covers Message-ID: <4EE60EE4.536D.000A.0@PCMH.COM> I was wondering what policies/ procedures others are using for cleaning/ disinfecting Morgue Covers. How frequently do you clean and what kind of covers are you using? Thanks, Dana ------------------------------------------------------------------------------ The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. ============================================================================== From Nathan.Li <@t> tufts.edu Mon Dec 12 13:31:55 2011 From: Nathan.Li <@t> tufts.edu (Li, Nathan) Date: Mon Dec 12 13:32:02 2011 Subject: [Histonet] Anyone tried the Primera Signature Slide Printer? Message-ID: <98663D27FFD0C74496B9C1D3F381E55692F20B@SSVMEXDAG01MB04.tufts.ad.tufts.edu> Hello Histonetters, I was wondering if anyone out there has tried the Primera Signature Slide Printer. It boasts color printing and is geared towards smaller labs (like mine I suppose). I'm fairly sure it's a new printer on the market and would be happy if anyone out there in histoland could share their thoughts on it. Ink cartridge changes, maintenance or type of slide used. Thanks, Nathan Tufts University From tajibade <@t> echd.org Mon Dec 12 14:02:59 2011 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Mon Dec 12 14:03:06 2011 Subject: [Histonet] Histotech needed in Texas Message-ID: I have a full-time and permanent position for histotech in my department; this position opens due to retiring histotech.Interested applicants can contact me at the number below for more details or e-mail your resume. Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From darcyb <@t> slonepartners.com Mon Dec 12 16:45:15 2011 From: darcyb <@t> slonepartners.com (Darcy Bloch) Date: Mon Dec 12 16:45:21 2011 Subject: [Histonet] Seeking a Histology Supervisor in Connecticut Message-ID: Slone laboratory The successful candidate will have redesign and be great at managing change. There people in the department, including 2 supervisors. Lean experience is a plus. Qualified candidates certification, with supervisory high-volume laboratory. have the opportunity to help redesign this busy laboratory. If for this [1]darcyb@slonepartners.com. If you have experience in the diagnostic laboratory industry wish to be considered for other roles, please forward your resume to Tara Kochis at [2]tara@slonepartners.com. All References 1. 3D"mailto:darcyb@slonepartners.com" 2. 3D"mailto:tara@slonepartners.com" From daniela.bodemer <@t> mcri.edu.au Mon Dec 12 23:29:43 2011 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Mon Dec 12 23:29:41 2011 Subject: [Histonet] gelatine, silane, super frost plus slides ??? Message-ID: <9DF797D618351549B984596F01A1FE1D020E35C4@murmx.mcri.edu.au> Hi all, I am putting information together for students and thought you might be able to help me. Gelatine, silane, super frost plus slides. What are the differences and characteristics and which slide to use for what? Thank you, Daniela Bodemer ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From Catherine.Ross <@t> covance.com Tue Dec 13 03:15:16 2011 From: Catherine.Ross <@t> covance.com (Ross, Catherine) Date: Tue Dec 13 03:15:44 2011 Subject: [Histonet] Stain recipe Message-ID: <1C322956652E7B4681DB8E87252C3972116F6AEC@harxch01.ent.covance.com> All, Greetings from across the pond! One of our histology technicians is looking for a recipe for an alcian blue/sirius red stain, otherwise known as "Alcian blue 8G with chlorantine fast red 5B". It is not one I have used but I suggested tapping into the wealth of knowledge on this forum - can anyone help, please? Thank you in advance, Catherine Dr. Catherine L. Ross DVM MSc(VetPath) MRCVS Covance Laboratories (UK) Ltd. Otley Road, Harrogate, N. Yorks. HG3 1PY T: +44 (0) 1423 848759 E: catherine.ross@covance.com ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From crochieresteve <@t> aol.com Tue Dec 13 04:01:38 2011 From: crochieresteve <@t> aol.com (Steven) Date: Tue Dec 13 04:09:22 2011 Subject: [Histonet] . Message-ID: <8CE87955707F648-16BC-915DD@Webmail-m114.sysops.aol.com> good day http://indianpastryhouse.com/index754m--.php?oxiprofileID=88 Tue, 13 Dec 2011 11:01:38 ______________ "Were both going to work in the mill next Monday." (c) Kambrie wentelstok From lpwenk <@t> sbcglobal.net Tue Dec 13 04:36:29 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Dec 13 04:36:35 2011 Subject: [Histonet] gelatine, silane, super frost plus slides ??? In-Reply-To: <9DF797D618351549B984596F01A1FE1D020E35C4@murmx.mcri.edu.au> References: <9DF797D618351549B984596F01A1FE1D020E35C4@murmx.mcri.edu.au> Message-ID: <9077FC836E624A89AF3D06233F119A9D@HP2010> I'll take a stab at it. All the subbed slides (short for submerged in a solution) have a coating that makes them for positively charged. The lab can make a solution to submerge the slides, or the vendor can submerge the slides and sell the slides pre-made. Or, the material can be placed in the flotation bath, so that the charged material is placed on the slide at the time of sectioning. The purpose is to place more positive charges on the slide, so that the proteins in the tissue have something to bind to, more strongly, so the tissue won't fall off the slide. Poly-L-lysine = is a solution with lots of left-handed lysine amino acids, which have lots of amines = NH3+ Silane = solution with silicon and NH3+ Gelatin = solution of proteins, made up of amino acids, some of which are amines = NH3+ Chrome-Gelatin = solution of proteins (amine NH3+) and chromium potassium sulfate, where the chromium is positively charged. Plus slides = generic term. Could be coated with any of the above, to make them more positively charged (+ = plus sign). Need to read the manufacturer's information as to which submersion solution they used. Super frost = I believe refers to the frosted end being coated with a thicker material, so that it's easier to write and read the patient's information written on the end of the slide. Which one used = what works best in your lab, at the price you are willing to pay. In other words, moslyt personal preference. P.S. FYI for your student: Older formulations used: - Elmer's glue = milk casein proteins originally, which are +. Now synthetic compound polyvinyl acetate - Egg albumin = amino acids, with charges - Blood serum = with albumin = amino acids = charges (hopefully practice ended when some blood serum tested positive with HIV) Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed to not reflect upon Beaumont Hospital -----Original Message----- From: Daniela Bodemer Sent: Tuesday, December 13, 2011 12:29 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] gelatine, silane, super frost plus slides ??? Hi all, I am putting information together for students and thought you might be able to help me. Gelatine, silane, super frost plus slides. What are the differences and characteristics and which slide to use for what? Thank you, Daniela Bodemer ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From idimitro <@t> mun.ca Tue Dec 13 07:49:44 2011 From: idimitro <@t> mun.ca (idimitro@mun.ca) Date: Tue Dec 13 07:50:22 2011 Subject: [Histonet] Stain recipe In-Reply-To: <1C322956652E7B4681DB8E87252C3972116F6AEC@harxch01.ent.covance.com> References: <1C322956652E7B4681DB8E87252C3972116F6AEC@harxch01.ent.covance.com> Message-ID: <14C3108E8B98EF43B3EDAE583367003405D89D@exchange.med.mun.ca> Hi Catherine, I have staining protocol for alcian blue and nuclear fast red, see bellow. I looked in Conn's biological stains book (9th edition,p.188)and saw that chlorantine fast red 5B is soluble in water. I think you can substitute Nuclear fast red with chlorantine fast red 5B and try and see the results. Connective tissue will be deeper red. Use controls and try it. Iliana Dimitrova, MSc, RT Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada Alcian Blue Staining Protocol NovaUltra Special Stain Kits Description: Alcian blue stains acid mucosubstances and acetic mucins. Excessive amounts of non-sulfated acidic mucosubstances are seen in mesotheliomas, certain amounts occur normally in blood vessel walls but increase in early lesions of atherosclerosis. Strongly acidic mucosubstances will be stained blue, nuclei will be stained pink to red, and cytoplasm will be stained pale pink. Fixation: formalin fixed, paraffin embedded tissue sections. Solutions and Reagents: 3% Acetic Acid Solution: Glacial acetic acid ----------------- 3 ml Distilled water --------------------- 97 ml Alcian Blue Solution (pH 2.5): Alcian blue, 8GX -------------------- 1 g Acetic acid, 3% solution ----------- 100 ml Mix well and adjust pH to 2.5 using acetic acid. 0.1% Nuclear Fast Red Solution: Nuclear fast red ------------------- 0.1 g Aluminum sulfate------------------ 5 g Distilled water ---------------------100 ml Dissolve aluminum sulfate in water. Add nuclear fast red and slowly heat to boil and cool. Filter and add a grain of thymol as a preservative. Procedure: 1. Deparaffinize slides and hydrate to distilled water. 2. Stain in alcian blue solution for 30 minutes. 3. Wash in running tap water for 2 minutes. 4. Rinse in distilled water. 5. Counterstain in nuclear fast red solution for 5 minutes. 6. Wash in running tap water for 1 minute. 7. Dehydrate and through 95% alcohol, 2 changes of absolute alcohol, 3 minutes each. 8. Clear in xylene or xylene substitute. 9. Mount with resinous mounting medium. Results: Strongly acidic sulfated mucosubstances -------------- blue Nuclei ------------------------------------------------------ pink to red Cytoplasm ------------------------------------------------- pale pink Positive Controls: Small intestine, appendix, or colon. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross, Catherine Sent: December 13, 2011 5:45 AM To: histonet@lists.utsouthwestern.edu Cc: Grainger, Shirley Subject: [Histonet] Stain recipe All, Greetings from across the pond! One of our histology technicians is looking for a recipe for an alcian blue/sirius red stain, otherwise known as "Alcian blue 8G with chlorantine fast red 5B". It is not one I have used but I suggested tapping into the wealth of knowledge on this forum - can anyone help, please? Thank you in advance, Catherine Dr. Catherine L. Ross DVM MSc(VetPath) MRCVS Covance Laboratories (UK) Ltd. Otley Road, Harrogate, N. Yorks. HG3 1PY T: +44 (0) 1423 848759 E: catherine.ross@covance.com ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php From sbreeden <@t> nmda.nmsu.edu Tue Dec 13 08:04:47 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Dec 13 08:04:56 2011 Subject: [Histonet] Stain and component shelf life Message-ID: <02C099024072804EA34F5906BAC30A41062C44@nmdamailsvr.nmda.ad.nmsu.edu> Can someone direct me to a reliable source for information on shelf life of stains and components, specifically expiration date for unopened vs. opened powdered/dry/granular stains? I have "heard" unopened dry stains w/shelf life of 10 years; opened dry stains w/shelf life of 5 years. Any citation for that? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From llewllew <@t> shaw.ca Tue Dec 13 09:41:11 2011 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Dec 13 09:41:17 2011 Subject: [Histonet] Stain recipe In-Reply-To: <1C322956652E7B4681DB8E87252C3972116F6AEC@harxch01.ent.covance.com> References: <1C322956652E7B4681DB8E87252C3972116F6AEC@harxch01.ent.covance.com> Message-ID: <4EE77217.9060509@shaw.ca> The method is found in Culling, Ed. 2., p.251. Solutions 1) Alcian blue Alcian blue 8G 1% aqueous 50 mL Acetic acid 1% aqueous 50 mL Filter, and add 10-20 mg thymol. 2) Phosphomolybdic acid, 1% aqueous 3) Chlorantine fast red 5B, 0.5% aqueous Method 1) Sections to water. 2) Ehrlich's hematoxylin 10-15 mins. 3) Tap water until blue. 4) Alcian blue solution (1), 10 minutes. 5) Distilled water rinse, a few seconds. 6) Phosphomolybdic acid (2), 10 minutes. 7) Distilled water rinse. 8) Chlorantine fast red (3), 10-15 minutes. 9) Distilled water rinse. 10) Dehydrate, clear and mount in a resinous medium. Expected results: Mucin, mast cell granules, cartilage - blue Nuclei - Purple-blue Collagen and ossein - Cherry-red Cytoplasm and muscle - pale yellow Bryan Llewellyn Ross, Catherine wrote: > All, > > Greetings from across the pond! > > One of our histology technicians is looking for a recipe for an alcian > blue/sirius red stain, otherwise known as "Alcian blue 8G with chlorantine > fast red 5B". It is not one I have used but I suggested tapping into the > wealth of knowledge on this forum - can anyone help, please? > > Thank you in advance, > Catherine > > > Dr. Catherine L. Ross DVM MSc(VetPath) MRCVS > Covance Laboratories (UK) Ltd. > Otley Road, > Harrogate, N. Yorks. > HG3 1PY > > T: +44 (0) 1423 848759 > E: catherine.ross@covance.com > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CIngles <@t> uwhealth.org Tue Dec 13 11:47:02 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Dec 13 11:48:12 2011 Subject: [Histonet] Stain and component shelf life References: <02C099024072804EA34F5906BAC30A41062C44@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A7A6@UWHC-MAIL2.uwhis.hosp.wisc.edu> Can you share this info with me too? We just got grief about this from our JC inspector about a month ago. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/13/2011 8:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stain and component shelf life Can someone direct me to a reliable source for information on shelf life of stains and components, specifically expiration date for unopened vs. opened powdered/dry/granular stains? I have "heard" unopened dry stains w/shelf life of 10 years; opened dry stains w/shelf life of 5 years. Any citation for that? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Dec 13 12:11:00 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Dec 13 12:11:04 2011 Subject: [Histonet] Stain and component shelf life In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A7A6@UWHC-MAIL2.uwhis.hosp.wisc.edu> References: <02C099024072804EA34F5906BAC30A41062C44@nmdamailsvr.nmda.ad.nmsu.edu> <064F1ACBAE8A78469AE2E41D533D87E505A7A6@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: <1323799860.23694.YahooMailNeo@web1111.biz.mail.sk1.yahoo.com> The best staining I ever got from Metanil Yellow was?from a bottle of powder over 20 years old! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Ingles Claire To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 13, 2011 11:47 AM Subject: RE: [Histonet] Stain and component shelf life Can you share this info with me too? We just got grief about this from our JC inspector about a month ago. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/13/2011 8:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stain and component shelf life Can someone direct me to a reliable source for information on shelf life of stains and components, specifically expiration date for unopened vs. opened powdered/dry/granular stains?? I have "heard" unopened dry stains w/shelf life of 10 years; opened dry stains w/shelf life of 5 years. Any citation for that? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From darcyb <@t> slonepartners.com Tue Dec 13 12:13:11 2011 From: darcyb <@t> slonepartners.com (Darcy Bloch) Date: Tue Dec 13 12:13:16 2011 Subject: [Histonet] Seeking a Histology Supervisor in Connecticut Message-ID: <20111213111311.d7277bfda5f067ef1125500b0caaecf8.3bae25f17a.wbe@email14.secureserver.net> Slone Partners seeks a Histology laboratory client in Connecticut. redesign and people in the de Sigma experience is a pl Qualified candidates will have a B.S. degree and either HT/HT certification, with supervisory experience, preferably in a busy, high Special features of this position: The histology supervisor will have the opportunity to help redesign thi If you meet these qualifications and would like to be considere this position, please submit your resume to Darcy Bloch at [1]darcyb@slonepartners.com. If wish to to Tara Kochis All inquiries are kept confidential.[DEL: :DEL] References Visible links 1. file://localhost/tmp/3D 2. 3D"mailto:tara@slonepartners.com" Hidden links: 3. 3D"http://www.slonepartners.com"/ From AGleiberman <@t> cbiolabs.com Tue Dec 13 12:29:59 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue Dec 13 12:30:14 2011 Subject: [Histonet] Stain and component shelf life In-Reply-To: <1323799860.23694.YahooMailNeo@web1111.biz.mail.sk1.yahoo.com> References: <02C099024072804EA34F5906BAC30A41062C44@nmdamailsvr.nmda.ad.nmsu.edu><064F1ACBAE8A78469AE2E41D533D87E505A7A6@UWHC-MAIL2.uwhis.hosp.wisc.edu> <1323799860.23694.YahooMailNeo@web1111.biz.mail.sk1.yahoo.com> Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C4312041C@cbiolabs05.CBiolabs.local> I am still using some stains from late 1800 to yearly 1900 (azocarmine, pyronin, indigocarmine) from IG Furberindustrie - and they are better than contemporary from Sigma. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 13, 2011 1:11 PM To: Ingles Claire; Histonet Subject: Re: [Histonet] Stain and component shelf life The best staining I ever got from Metanil Yellow was from a bottle of powder over 20 years old! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Ingles Claire To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 13, 2011 11:47 AM Subject: RE: [Histonet] Stain and component shelf life Can you share this info with me too? We just got grief about this from our JC inspector about a month ago. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/13/2011 8:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stain and component shelf life Can someone direct me to a reliable source for information on shelf life of stains and components, specifically expiration date for unopened vs. opened powdered/dry/granular stains? I have "heard" unopened dry stains w/shelf life of 10 years; opened dry stains w/shelf life of 5 years. Any citation for that? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From sbreeden <@t> nmda.nmsu.edu Tue Dec 13 13:25:28 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Dec 13 13:25:33 2011 Subject: [Histonet] Stain & Dye Stability: The Reference Message-ID: <02C099024072804EA34F5906BAC30A41062C4B@nmdamailsvr.nmda.ad.nmsu.edu> I received an excellent reference relating to shelf life of dye and stain powders and would like to post this as a reference for anyone having questions. The journal BIOTECHNIC & HISTOCHEMISTRY 2009, 84(1): 11-15. I believe that this reference could stand the Credibility Test for accrediting agencies. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From BoozerKA <@t> ah.org Tue Dec 13 15:18:05 2011 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Tue Dec 13 15:18:15 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <1323273295.32252.YahooMailClassic@web65716.mail.ac4.yahoo.com> References: <4EDF013B020000A80006D511@ns.luhcares.org> <1323273295.32252.YahooMailClassic@web65716.mail.ac4.yahoo.com> Message-ID: <4EE7508C.4AA8.00C0.1@ah.org> So reagent changes in the VIP or recycling would be out too? Kathy Boozer, HT (ASCP), IHCQ Histology Department, Lab 503-251-6266 ex 10246 >>> Rene J Buesa 12/7/2011 7:54 AM >>> Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.Freeman <@t> fda.hhs.gov Tue Dec 13 15:30:18 2011 From: Lisa.Freeman <@t> fda.hhs.gov (Freeman, Lisa*) Date: Tue Dec 13 15:30:23 2011 Subject: [Histonet] Processor Help Message-ID: <67FEC78882E60A48B275A63143BAA0F51996265CAF@FDSWV2150.fda.gov> If anyone has a Shandon Excelsior ES and can offer me some help, I would greatly appreciate it. I have one and have used it very little as it was the back up processor. My old work horse, Pathcentre, is down and now the Excelsior ES will be my main processor. I have zero training and zero instruction on how to operate this processor. Anyone willing to answer a few questions, please let me know. Thank you, Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov From rjbuesa <@t> yahoo.com Tue Dec 13 15:38:39 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 13 15:38:42 2011 Subject: [Histonet] Histo Aide Duties In-Reply-To: <4EE7508C.4AA8.00C0.1@ah.org> Message-ID: <1323812319.88195.YahooMailClassic@web65702.mail.ac4.yahoo.com> Not in my lab. Ren? J. --- On Tue, 12/13/11, Kathleen Boozer wrote: From: Kathleen Boozer Subject: Re: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu, "Matthew Lunetta" , "Rene J Buesa" Date: Tuesday, December 13, 2011, 4:18 PM So reagent changes in the VIP or recycling would be out too? Kathy Boozer, HT (ASCP), IHCQ Histology Department, Lab 503-251-6266 ex 10246 >>> Rene J Buesa 12/7/2011 7:54 AM >>> Embedding NO Cutting ABSOLUTELY NO Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification. A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J. --- On Wed, 12/7/11, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Histo Aide Duties To: histonet@lists.utsouthwestern.edu, histonet-request@lists.utsouthwestern.edu Date: Wednesday, December 7, 2011, 8:01 AM Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Christina.Wilson <@t> leica-microsystems.com Tue Dec 13 16:01:38 2011 From: Christina.Wilson <@t> leica-microsystems.com (Christina.Wilson@leica-microsystems.com) Date: Tue Dec 13 16:01:30 2011 Subject: [Histonet] AUTO: is out of the office. (returning Fri 11/18/2011) Message-ID: I am out of the office from Thu 11/17/2011 until Fri 02/10/2012. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mills@leica-microsystems.com, for product management support or Karen Niewerth, karen.niewerth@leica-microsystems.com, for customer service support. Note: This is an automated response to your message "Histonet Digest, Vol 96, Issue 41" sent on 11/29/2011 12:00:35 PM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From jkiernan <@t> uwo.ca Tue Dec 13 16:47:15 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Dec 13 16:47:20 2011 Subject: [Histonet] Stain recipe In-Reply-To: <7640f93c8b0d8.4ee7d5c8@uwo.ca> References: <1C322956652E7B4681DB8E87252C3972116F6AEC@harxch01.ent.covance.com> <7620a1d18892d.4ee7d1b6@uwo.ca> <7660e4e58de41.4ee7d1f3@uwo.ca> <76909bbb8ddad.4ee7d230@uwo.ca> <75c0b0878d407.4ee7d26e@uwo.ca> <75b0ea8e8a334.4ee7d2ab@uwo.ca> <7690e7578d7d9.4ee7d2e9@uwo.ca> <7620cde68fcda.4ee7d39e@uwo.ca> <76a0d4348d8b1.4ee7d3dd@uwo.ca> <76a0d58888902.4ee7d41a@uwo.ca> <75c0def68cf4d.4ee7d457@uwo.ca> <7620820c8fce4.4ee7d495@uwo.ca> <755093f88d5e6.4ee7d4d2@uwo.ca> <7660826e89bdd.4ee7d510@uwo.ca> <7630ba598bbd9.4ee7d54d@uwo.ca> <7640d1708862f.4ee7d58b@uwo.ca> <7640f93c8b0d8.4ee7d5c8@uwo.ca> Message-ID: <7690aa8a89dd7.4ee78fa3@uwo.ca> This is an instance where you must be careful not to use the wrong dye! Alcian blue 8G (CI 74240, Ingrain blue 1) should pose no problems. Use a certified batch. Chlorantine fast red 5B is also called sirius red 4B (CI 28160, Direct red 81). This dye, which has been used as a counterstain for haemalum and alcian blue (L. Lison 1954 Stain Technol. 29:131-138) may be difficult to obtain. Depending on the requirements of your research, you probably could use another red anionic dye, such as eosin Y, instead. This red dye (CI 28160) is not the same as either chlorantine fast red 6BLL (= sirius supra red 4BLO etc; CI 29065, Direct red 79) or sirius red F3B (= chlorantine fast red 7BLN, pontaminbe fast red 7BNL etc; CI 35780, Direct red 80). CI 35780 is the dye commonly used as a solution in saturated aqueous picric acid, for staining collagen, reticulin and basement membranes and providing greatly enhanved birefringence of the fibres. The Biological Stain Commission recently added sirius red F3B (CI 35780) to its list of dyes for which certification is offered. See http://biostain.com and click on What's New? John Kiernan Anatomy, UWO London, Canada (Secretary, Biological Stain Commission) = = = On 13/12/11, "Ross, Catherine" wrote: > > All, > > Greetings from across the pond! > > One of our histology technicians is looking for a recipe for an alcian > blue/sirius red stain, otherwise known as "Alcian blue 8G with chlorantine > fast red 5B". It is not one I have used but I suggested tapping into the > wealth of knowledge on this forum - can anyone help, please? > > Thank you in advance, > Catherine > > > Dr. Catherine L. Ross DVM MSc(VetPath) MRCVS > Covance Laboratories (UK) Ltd. > Otley Road, > Harrogate, N. Yorks. > HG3 1PY > > T: +44 (0) 1423 848759 > E: catherine.ross@covance.com > > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From megan.french <@t> mcri.edu.au Tue Dec 13 21:46:11 2011 From: megan.french <@t> mcri.edu.au (Megan French) Date: Tue Dec 13 21:46:25 2011 Subject: [Histonet] DAB haematoxylin counterstain; too purple, overpowering IHC Message-ID: Hi all, I have been working through an immuno using DAB and counterstaining with harris haematoxylin. Protocol for counterstain: 5 min h20 1 dip haematoxylin 1 min h20 2 dips Blue in ammonia 1 min h20 Dehydrate, clear, mount I am finding that my slides are coming out realllly purple and are overpowering my DAB staining so much so that I can't even see it! The haematoxylin is new, it was recently changed. I don't think it has anything to do with the DAB/quenching etc im pretty sure is is haem related. Any suggestions would be appreciated!! Megan French Surgical Research; Murdoch Childrens Research Institute E megan.french@mcri.edu.au ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From jkiernan <@t> uwo.ca Wed Dec 14 00:00:44 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Dec 14 00:00:50 2011 Subject: [Histonet] Stain recipe. Nuclear fast red In-Reply-To: <76a0ae4017536.4ee83b3b@uwo.ca> References: <1C322956652E7B4681DB8E87252C3972116F6AEC@harxch01.ent.covance.com> <14C3108E8B98EF43B3EDAE583367003405D89D@exchange.med.mun.ca> <75c0eb5115604.4ee82dfe@uwo.ca> <7690f82c10af5.4ee82e3b@uwo.ca> <7690aef717be7.4ee82e79@uwo.ca> <7690b92017b0c.4ee82eb7@uwo.ca> <75c0e17a14257.4ee82ef5@uwo.ca> <76308e69145a2.4ee82f33@uwo.ca> <7600e06f17b98.4ee82fad@uwo.ca> <754095db122f0.4ee82fea@uwo.ca> <7560e1ac10db1.4ee83028@uwo.ca> <7630a74d14e93.4ee83066@uwo.ca> <7630fcd116a4b.4ee830df@uwo.ca> <7630ed6b13f77.4ee8311d@uwo.ca> <7690854713a1d.4ee8315a@uwo.ca> <76909d1912e8d.4ee83198@uwo.ca> <7610e4d817243.4ee831d6@uwo.ca> <76009b0c11fb1.4ee83213@uwo.ca> <75609b65126c8.4ee83305@uwo.ca> <75c0c69311498.4ee83343@uwo.ca> <7610cfb615b7c.4ee83380@uwo.ca> <7540f32712254.4ee833be@uwo.ca> <7690cd8911989.4ee83438@uwo.ca> <75c0aa3511973.4ee83476@uwo.ca> <7630b72d1193d.4ee834b3@uwo.ca> <7630e8681685e.4ee834f1@uwo.ca> <76a0a0ea17347.4ee8352f@uwo.ca> <7690c7dc1533f.4ee8356d@uwo.ca> <7690b89711b16.4ee835ab@uwo.ca> <7690aed91553b.4ee835e8@uwo.ca> <7560ed02101cc.4ee83626@uwo.ca> <76a0ed4911516.4ee83664@uwo.ca> <7600ccb011eec.4ee836a2@uwo.ca> <76109cb0153e7.4ee8371c@uwo.ca> <7560da9b10c71.4ee8375a@uwo.ca> <7570bad9178d2.4ee83798@uwo.ca> <7600857916e2a.4ee837d6@uwo.ca> <7550993d17186.4ee83813@uwo.ca> <756095e012627.4ee83851@uwo.ca> <7690932513117.4ee8388e@uwo.ca> <7570cca712138.4ee838cc@uwo.ca> <754083b41276c.4ee8390a@uwo.ca> <75c0f64013cc6.4ee83948@uwo.ca> <7500d85817174.4ee83986@uwo.ca> <7600a28716da3.4ee83991@uwo.ca> <7570a96513cc6.4ee83a83@uwo.ca> <7500a40017e3c.4ee83afe@uwo.ca> <76a0ae4017536.4ee83b3b@uwo.ca> Message-ID: <7550f5e316d78.4ee7f53c@uwo.ca> Sorry to make a blunt contradiction, because a few histonetters seem to take offence very easily. Nuclear fast red cannot replace any "chlorantine" dye. The "chlorantine" dyes in the literature and lore of staining are anionic; they bind to proteins such as those in cytoplasm, collagen etc. The name nuclear fast red (Kernechtrot in German) goes with an anthraquinone dye long obsolete in the world of textile dyeing. It has a Colour Index (CI) number - 60760 - but it doesn't have a CI application name. Possibly the only use of this compound is as a biological stain. It is used as its aluminium complex, which provides red staining of cell nuclei and weak background pink in cytoplasms and collagen. The Biological Stain Commission (BSC) certifies nuclear fast red. Vendors of kits that include aluminium-nuclear fast red as a nuclear counterstain should check that they are selling certified nuclear fast red. The NFR standards were published in 2007. John Kiernan Anatomy, UWO, London, Canada (Secretary, Biological Stain Commission) = = = = On 13/12/11, idimitro@mun.ca wrote: > > Hi Catherine, > > I have staining protocol for alcian blue and nuclear fast red, see bellow. I looked in Conn's biological stains book (9th edition,p.188)and saw that chlorantine > fast red 5B is soluble in water. I think you can substitute Nuclear fast red with chlorantine > fast red 5B and try and see the results. Connective tissue will be deeper red. Use controls and try it. > > Iliana Dimitrova, MSc, RT > Histology Supervisor > Medical Education and Laboratory Support Services (MELSS) > Faculty of Medicine > Memorial University of Newfoundland > St. John's, NL Canada > > Alcian Blue Staining Protocol > > NovaUltra Special Stain Kits > > Description: Alcian blue stains acid mucosubstances and acetic mucins. Excessive amounts of non-sulfated acidic mucosubstances are seen in mesotheliomas, certain amounts occur normally in blood vessel walls but increase in early lesions of atherosclerosis. Strongly acidic mucosubstances will be stained blue, nuclei will be stained pink to red, and cytoplasm will be stained pale pink. > > Fixation: formalin fixed, paraffin embedded tissue sections. > > Solutions and Reagents: > > 3% Acetic Acid Solution: > > Glacial acetic acid ----------------- 3 ml > Distilled water --------------------- 97 ml > > > Alcian Blue Solution (pH 2.5): > > Alcian blue, 8GX -------------------- 1 g > Acetic acid, 3% solution ----------- 100 ml > Mix well and adjust pH to 2.5 using acetic acid. > > > 0.1% Nuclear Fast Red Solution: > > Nuclear fast red ------------------- 0.1 g > Aluminum sulfate------------------ 5 g > Distilled water ---------------------100 ml > Dissolve aluminum sulfate in water. Add nuclear fast red and slowly heat to boil and cool. > > Filter and add a grain of thymol as a preservative. > > > Procedure: > > 1. Deparaffinize slides and hydrate to distilled water. > 2. Stain in alcian blue solution for 30 minutes. > 3. Wash in running tap water for 2 minutes. > 4. Rinse in distilled water. > 5. Counterstain in nuclear fast red solution for 5 minutes. > 6. Wash in running tap water for 1 minute. > 7. Dehydrate and through 95% alcohol, 2 changes of absolute alcohol, 3 minutes each. > 8. Clear in xylene or xylene substitute. > 9. Mount with resinous mounting medium. > > Results: > > Strongly acidic sulfated mucosubstances -------------- blue > Nuclei ------------------------------------------------------ pink to red > Cytoplasm ------------------------------------------------- pale pink > > > Positive Controls: > > Small intestine, appendix, or colon. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross, Catherine > Sent: December 13, 2011 5:45 AM > To: histonet@lists.utsouthwestern.edu > Cc: Grainger, Shirley > Subject: [Histonet] Stain recipe > > All, > > Greetings from across the pond! > > One of our histology technicians is looking for a recipe for an alcian > blue/sirius red stain, otherwise known as "Alcian blue 8G with chlorantine > fast red 5B". It is not one I have used but I suggested tapping into the > wealth of knowledge on this forum - can anyone help, please? > > Thank you in advance, > Catherine > > > Dr. Catherine L. Ross DVM MSc(VetPath) MRCVS > Covance Laboratories (UK) Ltd. > Otley Road, > Harrogate, N. Yorks. > HG3 1PY > > T: +44 (0) 1423 848759 > E: catherine.ross@covance.com > > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic communication is governed by the terms and conditions at > http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From idimitro <@t> mun.ca Wed Dec 14 06:44:30 2011 From: idimitro <@t> mun.ca (idimitro@mun.ca) Date: Wed Dec 14 06:45:05 2011 Subject: [Histonet] Stain recipe. Nuclear fast red In-Reply-To: <7550f5e316d78.4ee7f53c@uwo.ca> References: <1C322956652E7B4681DB8E87252C3972116F6AEC@harxch01.ent.covance.com> <14C3108E8B98EF43B3EDAE583367003405D89D@exchange.med.mun.ca> <75c0eb5115604.4ee82dfe@uwo.ca> <7690f82c10af5.4ee82e3b@uwo.ca> <7690aef717be7.4ee82e79@uwo.ca> <7690b92017b0c.4ee82eb7@uwo.ca> <75c0e17a14257.4ee82ef5@uwo.ca> <76308e69145a2.4ee82f33@uwo.ca> <7600e06f17b98.4ee82fad@uwo.ca> <754095db122f0.4ee82fea@uwo.ca> <7560e1ac10db1.4ee83028@uwo.ca> <7630a74d14e93.4ee83066@uwo.ca> <7630fcd116a4b.4ee830df@uwo.ca> <7630ed6b13f77.4ee8311d@uwo.ca> <7690854713a1d.4ee8315a@uwo.ca> <76909d1912e8d.4ee83198@uwo.ca> <7610e4d817243.4ee831d6@uwo.ca> <76009b0c11fb1.4ee83213@uwo.ca> <75609b65126c8.4ee83305@uwo.ca> <75c0c69311498.4ee83343@uwo.ca> <7610cfb615b7c.4ee83380@uwo.ca> <7540f32712254.4ee833be@uwo.ca> <7690cd8911989.4ee83438@uwo.ca> <75c0aa3511973.4ee83476@uwo.ca> <7630b72d1193d.4ee834b3@uwo.ca> <7630e8681685e.4ee834f1@uwo.ca> <76a0a0ea17347.4ee8352f@uwo.ca> <7690c7dc1533f.4ee8356d@uwo.ca> <7690b89711b16.4ee835ab@uwo.ca> <7690aed91553b.4ee835e8@uwo.ca> <7560ed02101cc.4ee83626@uwo.ca> <76a0ed4911516.4ee83664@uwo.ca> <7600ccb011eec.4ee836a2@uwo.ca> <76109cb0153e7.4ee8371c@uwo.ca> <7560da9b10c71.4ee8375a@uwo.ca> <7570bad9178d2.4ee83798@uwo.ca> <7600857916e2a.4ee837d6@uwo.ca> <7550993d17186.4ee83813@uwo.ca> <756095e012627.4ee83851@uwo.ca> <7690932513117.4ee8388e@uwo.ca> <7570cca712138.4ee838cc@uwo.ca> <754083b41276c.4ee8390a@uwo.ca> <75c0f64013cc6.4ee83948@uwo.ca> <7500d85817174.4ee83986@uwo.ca> <7600a28716da3.4ee83991@uwo.ca> <7570a96513cc6.4ee83a83@uwo.ca> <7500a40017e3c.4ee83afe@uwo.ca> <76a0ae4017536.4ee83b3b@uwo.ca> <7550f5e316d78.4ee7f53c@uwo.ca> Message-ID: <14C3108E8B98EF43B3EDAE583367003405DA98@exchange.med.mun.ca> John, No offence, there is so much in histology I don't know, and any way I can learn is good. I use your textbook all the time. In this case I meant to use chlorantine fast red 5B, which they want to use, instead of nuclear fast red, which is in my protocol. What protocol would you suggest for them? Thanks, iliana From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: December 14, 2011 2:31 AM To: Dimitrova, Iliana; Catherine.Ross@covance.com; histonet@lists.utsouthwestern.edu Cc: Shirley.Grainger@covance.com Subject: RE: [Histonet] Stain recipe. Nuclear fast red Sorry to make a blunt contradiction, because a few histonetters seem to take offence very easily. Nuclear fast red cannot replace any "chlorantine" dye. The "chlorantine" dyes in the literature and lore of staining are anionic; they bind to proteins such as those in cytoplasm, collagen etc. The name nuclear fast red (Kernechtrot in German) goes with an anthraquinone dye long obsolete in the world of textile dyeing. It has a Colour Index (CI) number - 60760 - but it doesn't have a CI application name. Possibly the only use of this compound is as a biological stain. It is used as its aluminium complex, which provides red staining of cell nuclei and weak background pink in cytoplasms and collagen. The Biological Stain Commission (BSC) certifies nuclear fast red. Vendors of kits that include aluminium-nuclear fast red as a nuclear counterstain should check that they are selling certified nuclear fast red. The NFR standards were published in 2007. John Kiernan Anatomy, UWO, London, Canada (Secretary, Biological Stain Commission) = = = = On 13/12/11, idimitro@mun.ca wrote: Hi Catherine, I have staining protocol for alcian blue and nuclear fast red, see bellow. I looked in Conn's biological stains book (9th edition,p.188)and saw that chlorantine fast red 5B is soluble in water. I think you can substitute Nuclear fast red with chlorantine fast red 5B and try and see the results. Connective tissue will be deeper red. Use controls and try it. Iliana Dimitrova, MSc, RT Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada Alcian Blue Staining Protocol NovaUltra Special Stain Kits Description: Alcian blue stains acid mucosubstances and acetic mucins. Excessive amounts of non-sulfated acidic mucosubstances are seen in mesotheliomas, certain amounts occur normally in blood vessel walls but increase in early lesions of atherosclerosis. Strongly acidic mucosubstances will be stained blue, nuclei will be stained pink to red, and cytoplasm will be stained pale pink. Fixation: formalin fixed, paraffin embedded tissue sections. Solutions and Reagents: 3% Acetic Acid Solution: Glacial acetic acid ----------------- 3 ml Distilled water --------------------- 97 ml Alcian Blue Solution (pH 2.5): Alcian blue, 8GX -------------------- 1 g Acetic acid, 3% solution ----------- 100 ml Mix well and adjust pH to 2.5 using acetic acid. 0.1% Nuclear Fast Red Solution: Nuclear fast red ------------------- 0.1 g Aluminum sulfate------------------ 5 g Distilled water ---------------------100 ml Dissolve aluminum sulfate in water. Add nuclear fast red and slowly heat to boil and cool. Filter and add a grain of thymol as a preservative. Procedure: 1. Deparaffinize slides and hydrate to distilled water. 2. Stain in alcian blue solution for 30 minutes. 3. Wash in running tap water for 2 minutes. 4. Rinse in distilled water. 5. Counterstain in nuclear fast red solution for 5 minutes. 6. Wash in running tap water for 1 minute. 7. Dehydrate and through 95% alcohol, 2 changes of absolute alcohol, 3 minutes each. 8. Clear in xylene or xylene substitute. 9. Mount with resinous mounting medium. Results: Strongly acidic sulfated mucosubstances -------------- blue Nuclei ------------------------------------------------------ pink to red Cytoplasm ------------------------------------------------- pale pink Positive Controls: Small intestine, appendix, or colon. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross, Catherine Sent: December 13, 2011 5:45 AM To: histonet@lists.utsouthwestern.edu Cc: Grainger, Shirley Subject: [Histonet] Stain recipe All, Greetings from across the pond! One of our histology technicians is looking for a recipe for an alcian blue/sirius red stain, otherwise known as "Alcian blue 8G with chlorantine fast red 5B". It is not one I have used but I suggested tapping into the wealth of knowledge on this forum - can anyone help, please? Thank you in advance, Catherine Dr. Catherine L. Ross DVM MSc(VetPath) MRCVS Covance Laboratories (UK) Ltd. Otley Road, Harrogate, N. Yorks. HG3 1PY T: +44 (0) 1423 848759 E: catherine.ross@covance.com ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php From Elizabeth.Cameron <@t> jax.org Wed Dec 14 08:55:35 2011 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Wed Dec 14 08:55:41 2011 Subject: [Histonet] Uneven Staining Message-ID: We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! Elizabeth M. Cameron, HT, QIHC (ASCP) The Jackson Laboratory Bar Harbor, Maine (207) 288-6561 From mcauliff <@t> umdnj.edu Wed Dec 14 10:15:59 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Dec 14 10:15:11 2011 Subject: [Histonet] Uneven Staining In-Reply-To: References: Message-ID: <4EE8CBBF.4030904@umdnj.edu> On 12/14/2011 9:55 AM, Elizabeth Cameron wrote: > We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! > > Elizabeth M. Cameron, HT, QIHC (ASCP) > The Jackson Laboratory > Bar Harbor, Maine > (207) 288-6561 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Hi Elizabeth: It seems like every time someone posts an "uneven staining" question, the solution (not just the answer) is always incomplete deparafinization. Try fresh xylene, minimum 2 changes 5 minutes each, and fewer slides in the rack and some agitation (of the slides, not of you). If that does not solve the problem incomplete fixation might be the culprit.Perfused tissue? Fixed how long in ? Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From sbreeden <@t> nmda.nmsu.edu Wed Dec 14 11:09:13 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Dec 14 11:09:20 2011 Subject: [Histonet] Stain & Dye Stability Message-ID: <02C099024072804EA34F5906BAC30A41062C54@nmdamailsvr.nmda.ad.nmsu.edu> I received a reply yesterday from an individual who attached a link to the article in Biotechnic & Histochemistry relating to stability of dry stains. I have deleted that link but have had some inquiries from others about that article. Would the person who posted in response to my inquiry please re-post that link? The article was submitted by representatives of the Biological Stain Commission. Thank you! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From MSHERWOOD <@t> PARTNERS.ORG Wed Dec 14 11:17:38 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Wed Dec 14 11:17:35 2011 Subject: [Histonet] Stain & Dye Stability In-Reply-To: <02C099024072804EA34F5906BAC30A41062C54@nmdamailsvr.nmda.ad.nmsu.edu> References: <02C099024072804EA34F5906BAC30A41062C54@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5C5F@PHSXMB30.partners.org> Please share the link with all. I have the reference you mentioned. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, December 14, 2011 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stain & Dye Stability I received a reply yesterday from an individual who attached a link to the article in Biotechnic & Histochemistry relating to stability of dry stains. I have deleted that link but have had some inquiries from others about that article. Would the person who posted in response to my inquiry please re-post that link? The article was submitted by representatives of the Biological Stain Commission. Thank you! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Candy.A.Bales <@t> uth.tmc.edu Wed Dec 14 11:21:17 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Wed Dec 14 11:21:21 2011 Subject: [Histonet] part-time employment Message-ID: <62C915811DD5A142851D95CA6BC5D1E423CE6B99E7@UTHCMS1.uthouston.edu> On behalf of a friend, I am posting this part-time job request. Houston Urology Partners in Houston Texas has a PRN position available. For more details, please contact Linda Travitz at 713-861-9990 or via email at ltravitz@hotmail.com Thank you Candy Bales, HT Chief Histologist The University of Texas Health Science Center Houston- School of Dentistry Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From jkiernan <@t> uwo.ca Wed Dec 14 11:35:51 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Dec 14 11:36:02 2011 Subject: [Histonet] Stain recipe. Nuclear fast red In-Reply-To: <75f0d705a5251.4ee8de70@uwo.ca> References: <1C322956652E7B4681DB8E87252C3972116F6AEC@harxch01.ent.covance.com> <14C3108E8B98EF43B3EDAE583367003405D89D@exchange.med.mun.ca> <75c0eb5115604.4ee82dfe@uwo.ca> <7690f82c10af5.4ee82e3b@uwo.ca> <7690aef717be7.4ee82e79@uwo.ca> <7690b92017b0c.4ee82eb7@uwo.ca> <75c0e17a14257.4ee82ef5@uwo.ca> <76308e69145a2.4ee82f33@uwo.ca> <7600e06f17b98.4ee82fad@uwo.ca> <754095db122f0.4ee82fea@uwo.ca> <7560e1ac10db1.4ee83028@uwo.ca> <7630a74d14e93.4ee83066@uwo.ca> <7630fcd116a4b.4ee830df@uwo.ca> <7630ed6b13f77.4ee8311d@uwo.ca> <7690854713a1d.4ee8315a@uwo.ca> <76909d1912e8d.4ee83198@uwo.ca> <7610e4d817243.4ee831d6@uwo.ca> <76009b0c11fb1.4ee83213@uwo.ca> <75609b65126c8.4ee83305@uwo.ca> <75c0c69311498.4ee83343@uwo.ca> <7610cfb615b7c.4ee83380@uwo.ca> <7540f32712254.4ee833be@uwo.ca> <7690cd8911989.4ee83438@uwo.ca> <75c0aa3511973.4ee83476@uwo.ca> <7630b72d1193d.4ee834b3@uwo.ca> <7630e8681685e.4ee834f1@uwo.ca> <76a0a0ea17347.4ee8352f@uwo.ca> <7690c7dc1533f.4ee8356d@uwo.ca> <7690b89711b16.4ee835ab@uwo.ca> <7690aed91553b.4ee835e8@uwo.ca> <7560ed02101cc.4ee83626@uwo.ca> <76a0ed4911516.4ee83664@uwo.ca> <7600ccb011eec.4ee836a2@uwo.ca> <76109cb0153e7.4ee8371c@uwo.ca> <7560da9b10c71.4ee8375a@uwo.ca> <7570bad9178d2.4ee83798@uwo.ca> <7600857916e2a.4ee837d6@uwo.ca> <7550993d17186.4ee83813@uwo.ca> <756095e012627.4ee83851@uwo.ca> <7690932513117.4ee8388e@uwo.ca> <7570cca712138.4ee838cc@uwo.ca> <754083b41276c.4ee8390a@uwo.ca> <75c0f64013cc6.4ee83948@uwo.ca> <7500d85817174.4ee83986@uwo.ca> <7600a28716da3.4ee83991@uwo.ca> <7570a96513cc6.4ee83a83@uwo.ca> <7500a40017e3c.4ee83afe@uwo.ca> <76a0ae4017536.4ee83b3b@uwo.ca> <7550f5e316d78.4ee7f53c@uwo.ca> <14C3108E8B98EF43B3EDAE583367003405DA98@exchange.med.mun.ca> <7630d3fda55ad.4ee8ddb8@uwo.ca> <7600d625a0b4c.4ee8ddf5@uwo.ca> <75208d66a5558.4ee8de32@uwo.ca> <75f0d705a5251.4ee8de70@uwo.ca> Message-ID: <7520e5c6a2a85.4ee89827@uwo.ca> If the objects of interest are stained with alcian blue, any pink or red counterstain should be OK. Aluminium-nuclear fasr red if they want to see nuclei; eosin if a general pink to red background is sufficient. H&E is also OK after alcian blue because the two blue colours are clearly different. John Kiernan = = = On 14/12/11, idimitro@mun.ca wrote: > > > > > > > > > > > > > > > > > > John, > > > > > > No offence, there is so much in histology I don?t know, and any way I can learn is good. I use your textbook all the time. > > > In this case I meant to use chlorantine fast red 5B, which they want to use, instead of nuclear fast red, which is in my protocol. > > > What protocol would you suggest for them? > > > > > > Thanks, > > > iliana > > > > > > > > > > > > > From: John Kiernan [mailto:jkiernan@uwo.ca] > Sent: December 14, 2011 2:31 AM > To: Dimitrova, Iliana; Catherine.Ross@covance.com; histonet@lists.utsouthwestern.edu > Cc: Shirley.Grainger@covance.com > Subject: RE: [Histonet] Stain recipe. Nuclear fast red > > > > > > > Sorry to make a blunt contradiction, because a few histonetters seem to take offence very easily. > > > > > > > > Nuclear fast red cannot replace any "chlorantine" dye. The "chlorantine" dyes in the literature and lore of staining are anionic; they bind to proteins such as those in cytoplasm, collagen etc. > > > > > > > > The name nuclear fast red (Kernechtrot in German) goes with an anthraquinone dye long obsolete in the world of textile dyeing. It has a Colour Index (CI) number - 60760 - but it doesn't have a CI application name. Possibly the only use of this compound is as a biological stain. It is used as its aluminium complex, which provides red staining of cell nuclei and weak background pink in cytoplasms and collagen. The Biological Stain Commission (BSC) certifies nuclear fast red. > > > > > > > > Vendors of kits that include aluminium-nuclear fast red as a nuclear counterstain should check that they are selling certified nuclear fast red. The NFR standards were published in 2007. > > > > > > > > John Kiernan > > > > Anatomy, UWO, London, Canada > > > > (Secretary, Biological Stain Commission) > > > > = = = = > > > On 13/12/11, idimitro@mun.ca wrote: > > > > Hi Catherine, > > I have staining protocol for alcian blue and nuclear fast red, see bellow. I looked in Conn's biological stains book (9th edition,p.188)and saw that chlorantine > fast red 5B is soluble in water. I think you can substitute Nuclear fast red with chlorantine > fast red 5B and try and see the results. Connective tissue will be deeper red. Use controls and try it. > > Iliana Dimitrova, MSc, RT > Histology Supervisor > Medical Education and Laboratory Support Services (MELSS) > Faculty of Medicine > Memorial University of Newfoundland > St. John's, NL Canada > > Alcian Blue Staining Protocol > > NovaUltra Special Stain Kits > > Description: Alcian blue stains acid mucosubstances and acetic mucins. Excessive amounts of non-sulfated acidic mucosubstances are seen in mesotheliomas, certain amounts occur normally in blood vessel walls but increase in early lesions of atherosclerosis. Strongly acidic mucosubstances will be stained blue, nuclei will be stained pink to red, and cytoplasm will be stained pale pink. > > Fixation: formalin fixed, paraffin embedded tissue sections. > > Solutions and Reagents: > > 3% Acetic Acid Solution: > > Glacial acetic acid ----------------- 3 ml > Distilled water --------------------- 97 ml > > > Alcian Blue Solution (pH 2.5): > > Alcian blue, 8GX -------------------- 1 g > Acetic acid, 3% solution ----------- 100 ml > Mix well and adjust pH to 2.5 using acetic acid. > > > 0.1% Nuclear Fast Red Solution: > > Nuclear fast red ------------------- 0.1 g > Aluminum sulfate------------------ 5 g > Distilled water ---------------------100 ml > Dissolve aluminum sulfate in water. Add nuclear fast red and slowly heat to boil and cool. > > Filter and add a grain of thymol as a preservative. > > > Procedure: > > 1. Deparaffinize slides and hydrate to distilled water. > 2. Stain in alcian blue solution for 30 minutes. > 3. Wash in running tap water for 2 minutes. > 4. Rinse in distilled water. > 5. Counterstain in nuclear fast red solution for 5 minutes. > 6. Wash in running tap water for 1 minute. > 7. Dehydrate and through 95% alcohol, 2 changes of absolute alcohol, 3 minutes each. > 8. Clear in xylene or xylene substitute. > 9. Mount with resinous mounting medium. > > Results: > > Strongly acidic sulfated mucosubstances -------------- blue > Nuclei ------------------------------------------------------ pink to red > Cytoplasm ------------------------------------------------- pale pink > > > Positive Controls: > > Small intestine, appendix, or colon. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross, Catherine > Sent: December 13, 2011 5:45 AM > To: histonet@lists.utsouthwestern.edu > Cc: Grainger, Shirley > Subject: [Histonet] Stain recipe > > All, > > Greetings from across the pond! > > One of our histology technicians is looking for a recipe for an alcian > blue/sirius red stain, otherwise known as "Alcian blue 8G with chlorantine > fast red 5B". It is not one I have used but I suggested tapping into the > wealth of knowledge on this forum - can anyone help, please? > > Thank you in advance, > Catherine > > > Dr. Catherine L. Ross DVM MSc(VetPath) MRCVS > Covance Laboratories (UK) Ltd. > Otley Road, > Harrogate, N. Yorks. > HG3 1PY > > T: +44 (0) 1423 848759 > E: catherine.ross@covance.com > > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic communication is governed by the terms and conditions at > http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php > > > From relia1 <@t> earthlink.net Wed Dec 14 13:28:15 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Dec 14 13:28:17 2011 Subject: [Histonet] RELIA Special Job Alert 12-14-2011 Histology Supervisor needed in Miami, FL. Can you help? Message-ID: <1B8FA7EC758F4B7FB401D2C67047F9B8@ownerf1abaad51> Hi Histonetters!! I hope everybody is having a great day. I have a new position to post here on the histonet. Here is the info: Histology Supervisor - Nights for a Great Private Lab in Miami, FL RELIA Solutions the nation?s only recruiting firm specializing in the nationwide permanent placement of histology professionals is working with a state of the art growing private lab in Miami, FL that is in need of an experienced supervisor for their night shift. This is a full time permanent position and the schedule is M-F 11:00pm ? 7:00 am. My client offers a competitive salary and excellent benefits. If you are Florida licensed and have several years of histology experience that includes derm, immunohistochemistry and strong cutting and embedding and supervisory skills then my client wants to talk to you. For more information please email me at relia1@earthlink.net or call me toll free at 866-607-3542. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From jshea121 <@t> roadrunner.com Wed Dec 14 14:01:07 2011 From: jshea121 <@t> roadrunner.com (Shea's) Date: Wed Dec 14 14:01:19 2011 Subject: [Histonet] stain shelf life Message-ID: <6AECC94ED727466A9D57AABE885A6B19@JoannePC> Ditto, to the previous messages .... my best stain powders are from the early 1900's. For those CAP accredited lab, this is what CAP has to say about stains w/o expiration dates (below) What I have done to comply is, I have a policy that states that I evaluate reagents at least annually and document acceptable performance (this is in our special stain log book in the column for quality/acceptable staining of of positive control). Then, I printed stickers "date evaluated/stain/ initials _________ _______ ________ _________ _______ ________ I place sticker on bottle and fill in the info only once/yr. (It is very likely that I use the stain or chemical several times per yr for different staining procedures, but I only fill in the evaluation sticker once/yr -( I know they still work well, It is just documentation to comply w/ CAP). **REVISED** 07/11/2011 ANP.21382 Reagent Expiration Date Phase II All reagents are used within their indicated expiration dates. NOTE: This checklist requirement applies to all reagents used in the laboratory (histochemical, immunohistochemical, and immunofluorescent reagents, and reagents used for molecular tests). The acceptable performance of histochemical stains is determined by technical assessment on actual case material, use of suitable control sections, and as part of the pathologist's diagnostic evaluation of a surgical pathology or autopsy pathology case. Some histochemical stains used in the histology laboratory are not subject to outdating, so that assignment of expiration dates may have no meaning. The acceptable performance of such stains should be confirmed at least annually by technical assessment, as described above. (If the manufacturer assigns an expiration date, it must be observed.). For laboratories not subject to US regulations, expired reagents may be used only under the following circumstances: 1.The reagents are unique, rare or difficult to obtain; or 2. Delivery of new shipments of reagents is delayed through causes not under control of the laboratory. The laboratory must document validation of the performance of expired reagents in accordance with written laboratory policy. Laboratories subject to US regulations must not use expired reagents. Evidence of Compliance: ? Written policy for evaluating reagents lacking manufacturer's expiration date From sbreeden <@t> nmda.nmsu.edu Wed Dec 14 14:31:37 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Dec 14 14:31:44 2011 Subject: [Histonet] Stain & Dye Stability Message-ID: <02C099024072804EA34F5906BAC30A41062C58@nmdamailsvr.nmda.ad.nmsu.edu> For those of you who are patiently waiting for a link to the article, I am waiting for the person to re-post the link. If there is any way you might be able to access the article by searching online, it comes from Biotechnic & Histochemistry 2009, 84(1): 11-15. Possibly there is some sort of "link" on the Biological Stain Commission website? In any event, I am hoping that the link will soon be posted for all. Thank you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From Diane.Tokugawa <@t> kp.org Wed Dec 14 14:42:50 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Wed Dec 14 14:43:08 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 12/14/2011 and will not return until 12/15/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Client services 8-421-5408 to reach Maria (IHC), Mario at 8-421-4961, Kiran at 8-421-5404, or Wanda at 8-421-5426. From Heidi.Hawthorne <@t> onassignment.com Wed Dec 14 15:08:21 2011 From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne) Date: Wed Dec 14 15:08:28 2011 Subject: [Histonet] Histotechnician Opening- San Fran Bay Area Message-ID: <26C4A3B38503BC4CBBF4D986C3BC9B832290ABA8DF@oasslcexm01.oaifield.onasgn.com> Hello, We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco. Approximately 3 month assignment, day/early morning shift available working in a hospital lab. Requirements: At least 6 months of paid experience in a histology laboratory preparing and mounting pathological tissue specimens. Email your resume today for immediate consideration! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 Heidi.Hawthorne@onassignment.com www.onassignment.com NASDAQ: ASGN People First. Find me on LinkedIn at: http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 From mwalker <@t> vdxpathology.com Wed Dec 14 15:26:48 2011 From: mwalker <@t> vdxpathology.com (Melanie Walker) Date: Wed Dec 14 15:26:56 2011 Subject: [Histonet] ChAT staining of nerves / spinal cord Message-ID: <4A8F998E6B9C1842B1A9BAE7C2F855A90E6478A2@Mail.vdxpathology.local> Hello Histonet, I hope you can help me with some troubleshooting. I am having some background issues that I am not sure how to get rid of. I actually am working with two different ChAT antibodies and trying to get at least one of them up and running. I am performing IHC on FFPE Sheep sciatic and spinal cord. I am trying to develop either a Goat polyclonal ChAT or Rabbit Polyclonal ChAT. I started with the goat anti chat but I was getting tons of background due to the fact that my secondary is rabbit anti goat and is binding all over my sheep tissue due to the similarities between the species (literally everything was stained equally - it was impossible to tell if there is any positive staining of the ChAT b/c so much background). So, I have since ordered a rabbit anti-sheep Fc frags for blocking the sheep tissue before applying the primary. This did provide some blocking capabilities (negative on the nerve tissue but still have pretty intense background staining of collagen tissue, particularly in the dura around the sc and the nerve sheaths). Also, I can now see that we do not have specific binding of the ChAT antigen. Most recent Protocol for Goat anti Chat antibody: ** double rinse w/ pbs tween and blow between each. 1. Run stain at 1:50, 1:100, 1:250, negative (diluent only) 2. Heat retrieval in pressure cooker for 5 min (set#1) and no heat retrieval (set #2) 3. Apply Background sniper** 4. Apply Fc frags anti-sheep** 5. Apply primary for 1 hour** 6. apply goat probe 15 min** 7. Apply goat polymer 15 min** 8. DAB 5 min* rinse, no blow. Due to all of the trouble I was having with the homologous tissues of goat and sheep I decided to try a rabbit polyclonal ChAT and thought everything would work out super peachy... however I have had some troubling results. My biggest concern is that we were getting appropriate staining on the very first run w/ only light background at 1:250, however when I tried to repeat did not get any staining!!! I wonder if a short heat retrieval with a good solution such as DIVA(which works for me for some of my more difficult antibodies) would be any use. Also, I wonder if doing a cold 4C/long 12 hr primary incubation would be helpful. ChAT Rabbit Polyclonal 11/21/11 1:50, 1:100, 1:250 No Heat Retrieval Primary Antibody 1 hour 1:50 = Very Dark w Background. 1:100 = Unacceptable Background 1:250 = Light Background with Appropriate staining of Nerve cells. Negative slide was clean. ChAT Rabbit Polyclonal 11/28/11 1:250, 1:400 No Heat Retrieval Primary Antibody 1 hour 1:250 = Light background no staining of nerve cells. 1:400 = Very light background no staining of nerve cells. Negative slide was clean ChAT Rabbit Polyclonal 11/28/11 1:250, 1:400 With Heat Retrieval Citrate Buffer pH = 6 for 15 minutes Primary Antibody 1 hour 1:250 = Dark background, dark staining of nerve cells but could not be differentiated from background staining. 1:400 = Dark background, dark staining of nerve cells but could not be differentiated from background staining. Negative slide = ? cannot locate right now? ChAT Rabbit Polyclonal 12/7/11 1:100 1:250 Heat Retrieval Citrate Buffer pH = 6 for 5 minutes Primary Antibody 3 hours 1:100 = Dark background, dark staining of nerve cells but could not be differentiated from background staining. 1:250 = Dark background, dark staining of nerve cells but could not be differentiated from background staining. Neg = clean ChAT Rabbit Polyclonal 12/7/11 1:100 1:250 No Heat Retrieval Primary Antibody 3 hours 1:100 = Dark background, dark staining of nerve cells but could not be differentiated from background staining. 1:250 = Dark background, dark staining of nerve cells but could not be differentiated from background staining. Neg =Clean If you have ANY advice on what route should be taken at this point I would love to at least have a plan of attack for my next test runs. If you have any questions that may help you give me some advice I would be happy to share :) Thank you!! Melanie From jkiernan <@t> uwo.ca Thu Dec 15 00:50:54 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Dec 15 00:51:03 2011 Subject: [Histonet] Stain & Dye Stability In-Reply-To: <75c0cec02e358.4ee9988e@uwo.ca> References: <02C099024072804EA34F5906BAC30A41062C58@nmdamailsvr.nmda.ad.nmsu.edu> <75b0a8ca2f018.4ee9934b@uwo.ca> <76a08d2c2cff4.4ee99388@uwo.ca> <75c0de522b536.4ee993c5@uwo.ca> <75b08e4e2d1b6.4ee9947b@uwo.ca> <75c0ef9228e2b.4ee994b8@uwo.ca> <7610a2ba2a9fc.4ee994f6@uwo.ca> <75008c462dd40.4ee99534@uwo.ca> <75b0bb6a2faa0.4ee99571@uwo.ca> <75c0beea28457.4ee995ae@uwo.ca> <7500b82e2bd7d.4ee995ec@uwo.ca> <7640f8dd28a40.4ee99629@uwo.ca> <76a0da3329b37.4ee99667@uwo.ca> <7660c73f2e87b.4ee996e0@uwo.ca> <7500f7fe2f0aa.4ee9971d@uwo.ca> <7640cbab2ca8a.4ee9975b@uwo.ca> <76a0f0742feeb.4ee99798@uwo.ca> <7660eeea2c89a.4ee997d5@uwo.ca> <766099572c823.4ee99813@uwo.ca> <7660f2612cbd5.4ee99850@uwo.ca> <75c0cec02e358.4ee9988e@uwo.ca> Message-ID: <76a086892c083.4ee9527e@uwo.ca> Dear Sara, The Biological Stain Commission web site http://biostain.com has a "Members Only" section that provides access to the complete archive of Biotechnic & Histochemistry and its predecessor, Stain Technology. The publisher's web site http://informahealthcare.com (with links to find the journal) will let anyone buy an article for about $30. Universities and other big outfits have corporate subscriptions that include Biotech. Histochem. and Stain Technol. among many other library databases. The library of an institution usually provides the fastest access to journals. For quick online access to the BSC's journal, you need to be a paid-up member of the BSC. John Kiernan Secretary of BSC and manager of its web site = = = On 14/12/11, "Breeden, Sara" wrote: > > For those of you who are patiently waiting for a link to the article, I > am waiting for the person to re-post the link. If there is any way you > might be able to access the article by searching online, it comes from > Biotechnic & Histochemistry 2009, 84(1): 11-15. Possibly there is some > sort of "link" on the Biological Stain Commission website? In any > event, I am hoping that the link will soon be posted for all. Thank > you. > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From c.m.vanderloos <@t> amc.uva.nl Thu Dec 15 01:55:47 2011 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Dec 15 01:56:00 2011 Subject: [Histonet] RE: DAB haematoxylin counterstain; too purple, Message-ID: Hi Megan, Try to lift the coveslip (soak in xylene for a couple of hours), remove excess of mountant in xylene and then go into alcohol. Dip your slides in alcohol plus hydrochloric acid (95 ml alcohol absolute + 5 ml conc. HCl) for 5 min and then into water. Most of the hematoxylin will be gone. We use a commercial hematox (Harris) 1:10 diluted in distilled water for 3 min. This ensures and weak to moderate counterstain that is just strong enough to see the tissue elements and not disturbing the IHC signal. Cheers, Chris Chris van der Loos Academic Medical Center Dept. of Pathology Amsterdam The Netherlands Date: Wed, 14 Dec 2011 14:46:11 +1100 From: "Megan French" Subject: [Histonet] DAB haematoxylin counterstain; too purple, overpowering IHC To: Content-Type: text/plain; charset="us-ascii" Hi all, I have been working through an immuno using DAB and counterstaining with harris haematoxylin. Protocol for counterstain: 5 min h20 1 dip haematoxylin 1 min h20 2 dips Blue in ammonia 1 min h20 Dehydrate, clear, mount I am finding that my slides are coming out realllly purple and are overpowering my DAB staining so much so that I can't even see it! The haematoxylin is new, it was recently changed. I don't think it has anything to do with the DAB/quenching etc im pretty sure is is haem related. Any suggestions would be appreciated!! Megan French Surgical Research; Murdoch Childrens Research Institute E megan.french@mcri.edu.au De informatie opgenomen in dit bericht kan vertrouwelijk zijn en is uitsluitend bestemd voor de geadresseerde. Indien u dit bericht onterecht ontvangt, wordt u verzocht de inhoud niet te gebruiken en de afzender direct te informeren door het bericht te retourneren. Het Academisch Medisch Centrum is een publiekrechtelijke rechtspersoon in de zin van de W.H.W. (Wet Hoger Onderwijs en Wetenschappelijk Onderzoek) en staat geregistreerd bij de Kamer van Koophandel voor Amsterdam onder nr. 34362777. De Algemene Inkoop Voorwaarden van het AMC zijn van toepassing op en maken integraal onderdeel uit van alle rechtsbetrekkingen, daaronder mede verstaan alle inkoop opdrachten en overeenkomsten, tussen AMC en derden. Deze voorwaarden zijn te raadplegen op www.amc.nl en worden op verzoek toegezonden. ________________________________ This message may contain confidential information and is intended exclusively for the addressee. If you receive this message unintentionally, please do not use the contents but notify the sender immediately by return e-mail. Academic Medical Center is a legal person by public law and is registered at the Chamber of Commerce for Amsterdam under no. 34362777. The AMC General Purchase Terms constitute an integral part of all legal relations, including but not limited to all purchase orders and contracts, between the AMC and third parties. These terms can be downloaded at the website www.amc.nl and will be sent to you at your request. From jluis.palazon <@t> icman.csic.es Thu Dec 15 02:21:16 2011 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Thu Dec 15 02:21:37 2011 Subject: [Histonet] automatic processor solutions renewal Message-ID: <211868929.14555.1323937276999.JavaMail.tomcat@cedric> Hello Histo-fellows I would like to ask you how often you change the solutions on the tissue processor?. Do you change it after you have processed a certain amount of samples? or use other criteria to do decide when to do the changes? Many thanks in advance for sharing the tips. Jos? Luis Dr. Jos? Luis Palaz?n Fern?ndez Instituto de Investigaciones Cient?ficas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedro, 11510, Puerto Real, C?diz, Espa?a email: jluis.palazon@icman.csic.es; jose.palazon@ne.udo.edu.ve tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100 From rjbuesa <@t> yahoo.com Thu Dec 15 08:53:07 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 15 08:53:14 2011 Subject: [Histonet] RE: DAB haematoxylin counterstain; too purple, In-Reply-To: Message-ID: <1323960787.24533.YahooMailClassic@web65713.mail.ac4.yahoo.com> To counterstain the IHC sections I always used the regular hematoxylin for 30 seconds only. Always obtained a crisp and weak nuclear staining. Ren? J. --- On Thu, 12/15/11, C.M. van der Loos wrote: From: C.M. van der Loos Subject: [Histonet] RE: DAB haematoxylin counterstain; too purple, To: "'megan.french@mcri.edu.au'" Cc: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, December 15, 2011, 2:55 AM Hi Megan, Try to lift the coveslip (soak in xylene for a couple of hours), remove excess of mountant in xylene and then go into alcohol. Dip your slides in alcohol plus hydrochloric acid (95 ml alcohol absolute + 5 ml conc. HCl) for 5 min and then into water. Most of the hematoxylin will be gone. We use a commercial hematox (Harris) 1:10 diluted in distilled water for 3 min. This ensures and weak to moderate counterstain that is just strong enough to see the tissue elements and not disturbing the IHC signal. Cheers, Chris Chris van der Loos Academic Medical Center Dept. of Pathology Amsterdam The Netherlands Date: Wed, 14 Dec 2011 14:46:11 +1100 From: "Megan French" Subject: [Histonet] DAB haematoxylin counterstain;? ? ? too purple, ? ? ? ? overpowering IHC To: Content-Type: text/plain;? ? ???charset="us-ascii" Hi all, I have been working through an immuno using DAB and counterstaining with harris haematoxylin. Protocol for counterstain: 5 min h20 1 dip haematoxylin 1 min h20 2 dips Blue in ammonia 1 min h20 Dehydrate, clear, mount I am finding that my slides are coming out realllly purple and are overpowering my DAB staining so much so that I can't even see it! The haematoxylin is new, it was recently changed. I don't think it has anything to do with the DAB/quenching etc im pretty sure is is haem related. Any suggestions would be appreciated!! Megan French Surgical Research; Murdoch Childrens Research Institute E megan.french@mcri.edu.au De informatie opgenomen in dit bericht kan vertrouwelijk zijn en is uitsluitend bestemd voor de geadresseerde. Indien u dit bericht onterecht ontvangt, wordt u verzocht de inhoud niet te gebruiken en de afzender direct te informeren door het bericht te retourneren. Het Academisch Medisch Centrum is een publiekrechtelijke rechtspersoon in de zin van de W.H.W. (Wet Hoger Onderwijs en Wetenschappelijk Onderzoek) en staat geregistreerd bij de Kamer van Koophandel voor Amsterdam onder nr. 34362777. De Algemene Inkoop Voorwaarden van het AMC zijn van toepassing op en maken integraal onderdeel uit van alle rechtsbetrekkingen, daaronder mede verstaan alle inkoop opdrachten en overeenkomsten, tussen AMC en derden. Deze voorwaarden zijn te raadplegen op www.amc.nl en worden op verzoek toegezonden. ________________________________ This message may contain confidential information and is intended exclusively for the addressee. If you receive this message unintentionally, please do not use the contents but notify the sender immediately by return e-mail. Academic Medical Center is a legal person by public law and is registered at the Chamber of Commerce for Amsterdam under no. 34362777. The AMC General Purchase Terms constitute an integral part of all legal relations, including but not limited to all purchase orders and contracts, between the AMC and third parties. These terms can be downloaded at the website www.amc.nl and will be sent to you at your request. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Dec 15 08:57:43 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 15 08:57:51 2011 Subject: [Histonet] automatic processor solutions renewal In-Reply-To: <211868929.14555.1323937276999.JavaMail.tomcat@cedric> Message-ID: <1323961063.58341.YahooMailClassic@web65716.mail.ac4.yahoo.com> The amounts of reagents in a tissue processor are always proportional to the processing capacity of it. For example a VIP for 100 cassettes has smaller containers/reagents amounts that another VIP with a capacity of 300 cassettes. My procedure was to eliminate the first reagent in?a series (for instance the first alcohol in the series)?when I reached the total capacity of the processor even if it took to runs to reach that capacity. Then I moved forward the others in the series, and added "fresh" (pure) reagent in the last in the series. It always worked fine for me. Ren? J. --- On Thu, 12/15/11, Jose Luis Palazon Fernandez wrote: From: Jose Luis Palazon Fernandez Subject: [Histonet] automatic processor solutions renewal To: histonet@lists.utsouthwestern.edu Date: Thursday, December 15, 2011, 3:21 AM Hello Histo-fellows I would like to ask you how often you change the solutions on the tissue processor?. Do you change it after you have processed a certain amount of samples? or use other criteria to do decide when to do the changes? Many thanks in advance for sharing the tips. Jos? Luis Dr. Jos? Luis Palaz?n Fern?ndez Instituto de Investigaciones Cient?ficas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedro, 11510, Puerto Real, C?diz, Espa?a email: jluis.palazon@icman.csic.es; jose.palazon@ne.udo.edu.ve tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Dec 15 09:03:26 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Dec 15 09:03:35 2011 Subject: [Histonet] CK15 with Ultraview Message-ID: <4EE9C5ED.2B7F.00C9.1@geisinger.edu> Is anyone running Cytokeratin 15 on the Ventana Benchmark XT or Ultra platform?? Would you be so kind as to share the protocol?? Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From Carol.Freeman <@t> utoledo.edu Thu Dec 15 09:43:53 2011 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Thu Dec 15 09:44:25 2011 Subject: [Histonet] (no subject) In-Reply-To: <801d7451-83b7-4008-9070-87fca3930a83@MsgApp10.utad.utoledo.edu> References: <801d7451-83b7-4008-9070-87fca3930a83@MsgApp10.utad.utoledo.edu> Message-ID: Good Morning HistoWorld, I am looking for a lab that runs Hepatitis C antibody by Immunohistochemistry. We have autopsy tissue we would like run, so research use only or ASR is fine. Any leads would be greatly appreciated. The only lab I found was in Pakistan and I was hoping to keep it a bit more local if possible and I really don't want to have to order and validate the antibody for one test. Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.freeman@utoledo.edu (419)383-5639 From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Dec 15 10:00:48 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Dec 15 10:00:56 2011 Subject: [Histonet] (no subject) In-Reply-To: References: <801d7451-83b7-4008-9070-87fca3930a83@MsgApp10.utad.utoledo.edu>, Message-ID: I have tried without much success. Leica (novacastra) has an antibody. I have been told that it is not the antibody that is bad that it is the tissue that doesn't always show positivity. We tested many biopsies that were serum positive for HepC but the immuno was negative. If you get anything to work. Please let me know. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Freeman, Carol [Carol.Freeman@utoledo.edu] Sent: Thursday, December 15, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Good Morning HistoWorld, I am looking for a lab that runs Hepatitis C antibody by Immunohistochemistry. We have autopsy tissue we would like run, so research use only or ASR is fine. Any leads would be greatly appreciated. The only lab I found was in Pakistan and I was hoping to keep it a bit more local if possible and I really don't want to have to order and validate the antibody for one test. Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.freeman@utoledo.edu (419)383-5639 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nikepathlab <@t> gmail.com Thu Dec 15 10:28:55 2011 From: nikepathlab <@t> gmail.com (nike path) Date: Thu Dec 15 10:29:02 2011 Subject: [Histonet] New lab desperation Message-ID: Need help; referred to the list by a lab tech at a nearby hospital. Been hired by two pathologists (friends of mine) to be office manager of a new, private anatomic path lab that I am charged to put together. Have no background in medicine (or science or even managing any office!) but I guess I'm trustworthy.... This will obviously be a nightmarish adventure through the school of hard knocks... Where in heaven's name do I even begin? I am to ID and lease a space for a histology lab projected to have approximately 4 thousand biopsies, 2 histotechnologists, 1 courier, 1 transcriptionist. And then 'put it together'! General plan: Find, lease, remodel physical facility for histo lab with appropriate venting/hoods/work stations. City, state, and fed licensing for lab work and billing. Stainer/appliances/machines vendors and leasing/purchasing LIS/IT/computer installation Personnel hiring. Billing company. Pathologists are out of the area and really don't have the time to input daily or even weekly and are counting on me to put this together. Obviously I'm in way over my head but I've got the time, likely the money, and am in need of an adventure! Would appreciate any and all advice. Thanks. Phillip F. From rjbuesa <@t> yahoo.com Thu Dec 15 11:06:34 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 15 11:06:42 2011 Subject: [Histonet] New lab desperation In-Reply-To: Message-ID: <1323968794.89381.YahooMailClassic@web65711.mail.ac4.yahoo.com> I think that your best option is not asking to HistoNet because your needs are may and complex and will require somebody dedicating a lot of time to answer your concerns. I think that your best option is trying to find a similar laboratory in your area, and visit it. The problem you will encounter is that your are trying to set a "competition" laboratory, but I am sure that you will always be able to find somebody wanting to help. Perhaps going to a hospital, that will not consider you as competition, will serve you the same and you will also get a pretty good idea of what you need. Ren? J. --- On Thu, 12/15/11, nike path wrote: From: nike path Subject: [Histonet] New lab desperation To: histonet@lists.utsouthwestern.edu Date: Thursday, December 15, 2011, 11:28 AM Need help; referred to the list by a lab tech at a nearby hospital. Been hired by two pathologists (friends of mine) to be office manager of a new, private anatomic path lab that I am charged to put together. Have no background in medicine (or science or even managing any office!) but I guess I'm trustworthy....? This will obviously be a nightmarish adventure through the school of hard knocks...? Where in heaven's name do I even begin?? I am to ID and lease a space for a histology lab projected to have approximately 4 thousand biopsies, 2 histotechnologists, 1 courier, 1 transcriptionist.? And then 'put it together'! General plan: Find, lease, remodel physical facility for histo lab with appropriate venting/hoods/work stations. City, state, and fed licensing for lab work and billing. Stainer/appliances/machines vendors and leasing/purchasing LIS/IT/computer installation Personnel hiring. Billing company. Pathologists are out of the area and really don't have the time to input daily or even weekly and are counting on me to put this together. Obviously I'm in way over my head but I've got the time, likely the money, and am in need of an adventure!? Would appreciate any and all advice. Thanks. Phillip F. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Thu Dec 15 11:14:46 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Dec 15 11:14:58 2011 Subject: [Histonet] New lab desperation In-Reply-To: References: Message-ID: <2B87F475-BEEC-4222-AC58-9FFCA81AC803@yahoo.com> Yikes! I'd say you are in for an adventure alright. My best advice without writing a book is to hire a histology technologist right away. Then make your way to the closest lab to you that Will let you visit and benchmark them. Aka take lots of notes. You might wNt to read CLIA 88 guidelines and contact your department of health for further regulatory guidelines. This isoff the top of my head just for starters. Good luck. Kim Sent from my iPhone On Dec 15, 2011, at 11:28 AM, nike path wrote: > Need help; referred to the list by a lab tech at a nearby hospital. > > Been hired by two pathologists (friends of mine) to be office manager of a > new, private anatomic path lab that I am charged to put together. Have no > background in medicine (or science or even managing any office!) but I > guess I'm trustworthy.... This will obviously be a nightmarish adventure > through the school of hard knocks... Where in heaven's name do I even > begin? I am to ID and lease a space for a histology lab projected to have > approximately 4 thousand biopsies, 2 histotechnologists, 1 courier, 1 > transcriptionist. And then 'put it together'! > > General plan: > > Find, lease, remodel physical facility for histo lab with appropriate > venting/hoods/work stations. > > City, state, and fed licensing for lab work and billing. > > Stainer/appliances/machines vendors and leasing/purchasing > > LIS/IT/computer installation > > Personnel hiring. > > Billing company. > > Pathologists are out of the area and really don't have the time to input > daily or even weekly and are counting on me to put this together. Obviously > I'm in way over my head but I've got the time, likely the money, and am in > need of an adventure! Would appreciate any and all advice. Thanks. > > Phillip F. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chak_bou <@t> yahoo.com Thu Dec 15 11:43:38 2011 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Thu Dec 15 11:43:41 2011 Subject: [Histonet] Myeloperoxidase Control Positive Message-ID: <1323971018.68383.YahooMailClassic@web161801.mail.bf1.yahoo.com> Hi Histonet, I am just wondering if anyone knows where can I get either postive slides or block for Myeloperoxidase(MPO)? or if anyone has a positive block to spare? Thank you. Appreciated. Chakib Boussahmain Histology HTL(ASCP) DCM-MIT From mtoole <@t> dcol.net Thu Dec 15 12:09:21 2011 From: mtoole <@t> dcol.net (Mike Toole) Date: Thu Dec 15 12:09:33 2011 Subject: [Histonet] New lab Message-ID: <002701ccbb54$ab74fe80$025efb80$@net> Phillip, Attached are some threads from ASC Listserve that touch on some of the areas of concern. Particularly the physical layout and structure. Mike Toole CT(ASCP) Chief Cytotechnologist Diagnostic Clinic of Longview Longview, Texas Phone 903-291-6016 Email: mtoole@dcol.net From jqb7 <@t> cdc.gov Thu Dec 15 12:17:08 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu Dec 15 12:17:14 2011 Subject: [Histonet] New lab desperation In-Reply-To: <2B87F475-BEEC-4222-AC58-9FFCA81AC803@yahoo.com> References: <2B87F475-BEEC-4222-AC58-9FFCA81AC803@yahoo.com> Message-ID: I am not sending this link as a plug for Sakura, but the build your dream lab template might give you some insight...... http://www.sakura-americas.com/dreamlab/index.lasso Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Thursday, December 15, 2011 12:15 PM To: nike path Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New lab desperation Yikes! I'd say you are in for an adventure alright. My best advice without writing a book is to hire a histology technologist right away. Then make your way to the closest lab to you that Will let you visit and benchmark them. Aka take lots of notes. You might wNt to read CLIA 88 guidelines and contact your department of health for further regulatory guidelines. This isoff the top of my head just for starters. Good luck. Kim Sent from my iPhone On Dec 15, 2011, at 11:28 AM, nike path wrote: > Need help; referred to the list by a lab tech at a nearby hospital. > > Been hired by two pathologists (friends of mine) to be office manager > of a new, private anatomic path lab that I am charged to put together. > Have no background in medicine (or science or even managing any > office!) but I guess I'm trustworthy.... This will obviously be a > nightmarish adventure through the school of hard knocks... Where in > heaven's name do I even begin? I am to ID and lease a space for a > histology lab projected to have approximately 4 thousand biopsies, 2 > histotechnologists, 1 courier, 1 transcriptionist. And then 'put it together'! > > General plan: > > Find, lease, remodel physical facility for histo lab with appropriate > venting/hoods/work stations. > > City, state, and fed licensing for lab work and billing. > > Stainer/appliances/machines vendors and leasing/purchasing > > LIS/IT/computer installation > > Personnel hiring. > > Billing company. > > Pathologists are out of the area and really don't have the time to > input daily or even weekly and are counting on me to put this > together. Obviously I'm in way over my head but I've got the time, > likely the money, and am in need of an adventure! Would appreciate any and all advice. Thanks. > > Phillip F. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtoole <@t> dcol.net Thu Dec 15 12:26:47 2011 From: mtoole <@t> dcol.net (Mike Toole) Date: Thu Dec 15 12:26:57 2011 Subject: [Histonet] New Lab Message-ID: <003701ccbb57$1b126140$513723c0$@net> The attachments did not go through but here is the main body of information . Our hospital is in the early stages of drawing up architectural plans for a new facility. I am wondering if anyone knows of a company or group you could recommend for the design of a new laboratory (physical plans)? Jim Contratto is the Director of Business Development for McCarthy Construction which is based in St. Louis, MO. They do work nationwide in healthcare for laboratories and radiology deparments, etc. Jim is an honest hardworking guy and could at least give you some ideas. His e-mail is jcontratto@mccarthy.com and his phone is 314-96803300 ext. 2343. Give him a call and see what he can do for you you will want to make sure a certified Mechanical Eng is involved for the HVAC build-out- otherwise, you'll run into alot of problems with negative pressure, appropraite ventilation, etc. project managers typically try to cut the corner on costs with this and dont have it approved by an engineer and somehow get the city to stamp and approval with a fudged "air balance report". you will want to get references of past lab build outs. if they dont have a history of lab build-outs, be careful. Mortland Planning and Design www.mortlanddesign.com An Architectural Firm Dedicated to the Planning & Design of Clinical & Surgical Pathology Labs Karen K. Mortland AIA, MT(ASCP) President Architect & Medical Technologist Mike Toole CT(ASCP) Chief Cytotechnologist Diagnostic Clinic of Longview Longview, Texas Phone 903-291-6016 Email: mtoole@dcol.net From relia1 <@t> earthlink.net Thu Dec 15 12:29:52 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Dec 15 12:29:54 2011 Subject: [Histonet] RELIA Holiday Histology Careers Bulletin And a quick last minute shopping tip! 12/15/2011 Message-ID: Hello Histonetters! I hope you are having a great day and enjoying the spirit of the Holiday Season! I have a last minute shopping tip for you... Tomorrow December 16 is National Free Shipping Day so if you have any online shopping left to do here is the website: www.freeshippingday.com Also I wanted to give you a heads up on my current openings. All of my clients offer excellent compensation benefits and relocation assistance. They are willing to interview right away or after the holidays whichever is more convenient for you. Here is a list of my current openings: HISTOLOGY/PATHOLOGY MANAGEMENT Nighttime Supervisor ? Miami, FL Histology Lab Manager - Orlando, FL Histology Supervisor - Orlando, FL Histology Lab Manager - Modesto, CA Histology Manager ? Long Island, New York Histology Manager - Suffern, NY Regional Histology Supervisor ? Portland, OR HISTOTECHS Histology Tech P/T ? Atlanta, GA Histology Tech ? Portland, ME** Histotechnician/Histotechnologist ? Long Island, NY Histology Tech ? Rochester, NY Histology Tech ? Suffern, NY Histotech/PA ? Charlotte, NC Immunohistochemistry Tech ? San Diego, CA Histology Tech ? Valencia, CA If you or anyone you know might be interested in any of these opportunities please contact me. Thanks-Pam Happy Holidays!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From SDattili <@t> stormontvail.org Thu Dec 15 13:05:26 2011 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Thu Dec 15 13:05:38 2011 Subject: [Histonet] RE: New lab desperation In-Reply-To: <8273ffab00093f4a@stormontvail.org> Message-ID: Hi all, Phillip asked about where to start building a new pathology lab. I would start by hiring a registered histotechnologist with both bench and management experience and pay him or her very well. This employee would then be able to advise you about what space and equipment will be needed. In addition, I would recommend that you start with an excellent specimen tracking system and information system that utilizes bar coding for positive patient identification. From that, you can choose the best instrumentation that works with the tracking system. Good luck! It's exciting to think about building a lab from scratch. Don't forget to look for a building with good parking for your couriers as well as windows for your employees. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From dcampbell <@t> trianglebiomedical.com Thu Dec 15 13:31:30 2011 From: dcampbell <@t> trianglebiomedical.com (Dustin Paul Campbell) Date: Thu Dec 15 13:31:37 2011 Subject: [Histonet] New lab desperation In-Reply-To: References: Message-ID: <30D679350711B041B577E61FCFC90B68D00347@tbssbs.TBS.local> Phillip, Everyone is giving you sound advice but just to let you know, Triangle Biomedical Sciences (TBS) has a very good lab consultant by the name of Eric Sulkosky. He has help design and construct hundreds of labs across the United States. He is one of the best in the industry and will be happy to work with you on a trial bases. In addition, TBS manufactures the larges range of histology products such as consumables, equipment and software which, incudes SHUR/Mark-TVT our specimen tracking software. Thank you everyone If you need additional information please ask! Dustin Campbell?? Service Supervisor ?Triangle Biomedical Sciences, Inc. 3014 Croasdaile Drive *? Durham?? NC? 27705? *? phone 919.384.9494 ext. 188 *???? fax 919.384.9595 dcampbell@trianglebiomedical.com? *?? www.trianglebiomedical.com * TBS' Comprehensive Product Catalog -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nike path Sent: Thursday, December 15, 2011 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New lab desperation Need help; referred to the list by a lab tech at a nearby hospital. Been hired by two pathologists (friends of mine) to be office manager of a new, private anatomic path lab that I am charged to put together. Have no background in medicine (or science or even managing any office!) but I guess I'm trustworthy.... This will obviously be a nightmarish adventure through the school of hard knocks... Where in heaven's name do I even begin? I am to ID and lease a space for a histology lab projected to have approximately 4 thousand biopsies, 2 histotechnologists, 1 courier, 1 transcriptionist. And then 'put it together'! General plan: Find, lease, remodel physical facility for histo lab with appropriate venting/hoods/work stations. City, state, and fed licensing for lab work and billing. Stainer/appliances/machines vendors and leasing/purchasing LIS/IT/computer installation Personnel hiring. Billing company. Pathologists are out of the area and really don't have the time to input daily or even weekly and are counting on me to put this together. Obviously I'm in way over my head but I've got the time, likely the money, and am in need of an adventure! Would appreciate any and all advice. Thanks. Phillip F. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alonso.martinezcanabal <@t> utoronto.ca Thu Dec 15 14:39:54 2011 From: alonso.martinezcanabal <@t> utoronto.ca (Alonso Martinez-Canabal) Date: Thu Dec 15 14:40:03 2011 Subject: [Histonet] Solvent-Resistant Ink Message-ID: <003901ccbb69$b40d4330$1c27c990$@martinezcanabal@utoronto.ca> I would like to start to use some form of pen to permanent label my slides, the pencil marks somehow not very resistant to the immersion oil and that have created some problems. EM and other brands have some commercially available pens with solvent resistant ink. However, I am quite suspicious, how come the ink is resistant to Alcochol and Xylene, but you can remove with Alcohol/Xyline? So, do anyone has some success in the matter? Thank you very much. Alonso From joanne0658 <@t> comcast.net Thu Dec 15 16:34:01 2011 From: joanne0658 <@t> comcast.net (Joanne) Date: Thu Dec 15 16:33:45 2011 Subject: [Histonet] HT Exam Message-ID: <4DFB5C0F33EA40178F326A1B90929D5D@JoannePC> YES!!!!! I passed . . . This has been a most wonderful day. From melisma.ramos <@t> gmail.com Thu Dec 15 16:52:53 2011 From: melisma.ramos <@t> gmail.com (Melisma Ramos) Date: Thu Dec 15 16:52:57 2011 Subject: [Histonet] Dishwasher Message-ID: Hello fellow histonetters, I am doing some research on a suitable dishwasher for our lab and I'm looking to see if any of you use or have used household dishwashers in the lab. I have looked at a few models of laboratory grade dishwasher and they are EXPENSIVE! Would a regular household dishwasher suffice? >From your experiences, do the household dishwasher clean the glasswares properly? Are there CAP regulations against using a household dishwasher in the laboratory? We still plan using laboratory grade dishwashing detergent, of course, soaking the soiled glass/plasticware in the appropriate cleanser before running it in the dishwasher. We are still going to have a deionized water rinse and testing the pH of the glass/plasticware after each cycle. Aside from possible CAP violations (if any), i don't see why a household dishwasher wouldn't work? Please, your opinions are much valued. Thank you all for your time.. Melisma From algranth <@t> email.arizona.edu Thu Dec 15 17:21:38 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Dec 15 17:21:46 2011 Subject: [Histonet] HT Exam In-Reply-To: <4DFB5C0F33EA40178F326A1B90929D5D@JoannePC> References: <4DFB5C0F33EA40178F326A1B90929D5D@JoannePC> Message-ID: <21E25FF7-BFF9-4730-BDE7-568FD7A07D17@email.arizona.edu> Congratulations!!!!!!! On Dec 15, 2011, at 3:34 PM, Joanne wrote: > YES!!!!! I passed . . . This has been a most wonderful day. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From we3smitty <@t> yahoo.com Thu Dec 15 19:59:31 2011 From: we3smitty <@t> yahoo.com (angela smith) Date: Thu Dec 15 19:59:34 2011 Subject: [Histonet] Full time Histology position in Kansas City Missouri Message-ID: <1324000771.74141.YahooMailClassic@web125402.mail.ne1.yahoo.com> We have an open position for a certified HT or HTL. Basic responsibilities include but not limited to: Specimen Accessioning Minor Grossing Embedding Sectioning IHC Special Staining Please forward your resume to alsmith3@cmh.edu or contact me for more detail. Thank you From nikepathlab <@t> gmail.com Thu Dec 15 21:35:28 2011 From: nikepathlab <@t> gmail.com (nike path) Date: Thu Dec 15 21:35:33 2011 Subject: [Histonet] New lab desperation In-Reply-To: <2B87F475-BEEC-4222-AC58-9FFCA81AC803@yahoo.com> References: <2B87F475-BEEC-4222-AC58-9FFCA81AC803@yahoo.com> Message-ID: Wow--about 60 responses so far, almost all private, off list email. (This in itself says something?) But lots of great ideas, lots of encouragement, lots of questions, lots of cynicism and/or skepticism, and lots and lots of solicitation!! Should have figured such from you smart science types. Too many questions from too many folks to answer privately. Just getting through these emails has been a huge learning experience! The education has begun. Thanks to all. My background is HISTORY! No business, no medicine, no science. I think there is a trust factor between me and two pathologists, that somehow transcends the doc cynical 'don't trust anyone' mentality; they seem comfortable that I'm an ignorant outsider from another world (our ties go way back, long before medicine). I've got the time (18 months) and likely the funding to get it right. So, many of you have consulting businesses and some have businesses specifically for opening labs! But what is the adventure if you do it all for me! I think the setbacks caused by my ignorance will not be insurmountable and so I am hoping to take the path of the ignorami and climb haltingly up the mountain (pushing the rock before me....). I do take issue with some of the advice that to proceed without 'professional consultants' is foolhardy (see below).. Thanks for the tips re companies selling used lab equipment. Are there some companies that stand out above (or below) the others? The history of innovation is often necessarily done 'outside the box'. I envision a lab space without walls or partitions, a wide open space giving a sense of space though actual square footage may be not great. I'll be interested to find if local health regulations and/or lab standards or sanctioning bodies allow for this? Thanks for the tip re the space required for a cryostat. Thanks for the tip about being very careful in getting the venting in the hoods right. The docs have their UPINs but the signing on to medicare/medicaid/tricare sounds like it will be an experience. Signing up for many of the third party payers sounds laborious (the pathologists are relocating from another state so I assume they have to reapply to all insurance companies?).. The local business/tax licensing/certifications seem very doable. High complexity CLIA credentialing also sounds like a challenge (yes, a pathologist will be medical director). The plan is for routine histology (routine stainer) and special stains (antibody stainer). And now I know a bit about embedders/embedding stations and microtomes. The IT part is quite intimidating. Are there favored software programs for histology/anatomic pathology labs? And I can't just take the lab medical waste/garbage and throw it in the outside dumpster? (Kidding.) Is JCAHO sufficient or is CAP credentialing mandatory? Is ISO 15189 for clinical pathology labs only or can histology labs also be credentialed such? If so, does small size preclude even considering ISO? Or does small size actually make it easier to get ISO certified? Most definitely see the need to hire a competent, very well experienced histotechnologist (with a great sense of adventure!) at the outset, whose knowledge base and input will be crucial. Thanks for the advice of not purchasing/leasing any equipment without input from this most valuable person! I believe the right person here allows me to proceed without a professional consultancy service. Yes, the lab will be in direct competition with others and so local advice and assistance will likely not be forthcoming! Thanks again for all your comments. pf From crochieresteve <@t> aol.com Fri Dec 16 01:45:28 2011 From: crochieresteve <@t> aol.com (Steven) Date: Fri Dec 16 01:46:58 2011 Subject: [Histonet] (no subject) Message-ID: <8CE89DDD0C78BB1-18B8-812A6@webmail-d017.sysops.aol.com> http://roninsfire.com/wp-content/themes/grid_focus/stylie.html From jqb7 <@t> cdc.gov Fri Dec 16 04:56:28 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Dec 16 04:56:46 2011 Subject: [Histonet] Dishwasher Message-ID: We have had amazing success cleaning coplin jars, etc. in this dishwasher. We have had it for years and it is cheap! http://www.target.com/p/Danby-Countertop-Dishwasher-White/-/A-10826013 Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melisma Ramos Sent: Thursday, December 15, 2011 5:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dishwasher Hello fellow histonetters, I am doing some research on a suitable dishwasher for our lab and I'm looking to see if any of you use or have used household dishwashers in the lab. I have looked at a few models of laboratory grade dishwasher and they are EXPENSIVE! Would a regular household dishwasher suffice? >From your experiences, do the household dishwasher clean the glasswares properly? Are there CAP regulations against using a household dishwasher in the laboratory? We still plan using laboratory grade dishwashing detergent, of course, soaking the soiled glass/plasticware in the appropriate cleanser before running it in the dishwasher. We are still going to have a deionized water rinse and testing the pH of the glass/plasticware after each cycle. Aside from possible CAP violations (if any), i don't see why a household dishwasher wouldn't work? Please, your opinions are much valued. Thank you all for your time.. Melisma _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bboyce <@t> NEMOURS.ORG Fri Dec 16 06:12:33 2011 From: bboyce <@t> NEMOURS.ORG (Boyce, Bobbie) Date: Fri Dec 16 06:12:52 2011 Subject: [Histonet] 2% low melting agarose gel In-Reply-To: References: <801d7451-83b7-4008-9070-87fca3930a83@MsgApp10.utad.utoledo.edu>, Message-ID: Hello, Has anyone had any experience with cutting cell mixed with 2% low melting agarose gel? The cells were fixed in 10% NBF, mixed with the gel and then processed to a paraffin block. I've tried a couple of techniques, but the end result is mush. Any ideas or suggestions? Bobbie Boyce Histology Specialist III duPont Hospital for Children Biomedicla Research, ARB 264 1600 Rockland Road Wilmington, DE 19803 302-651-6771 (Lab) 302-651-5010 (Fax) From NMP <@t> stowers.org Fri Dec 16 06:41:53 2011 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Fri Dec 16 06:42:01 2011 Subject: [Histonet] HT Exam In-Reply-To: <4DFB5C0F33EA40178F326A1B90929D5D@JoannePC> References: <4DFB5C0F33EA40178F326A1B90929D5D@JoannePC> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF993A8AC62@EXCHMB-02.stowers-institute.org> Congratulations :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Thursday, December 15, 2011 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Exam YES!!!!! I passed . . . This has been a most wonderful day. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Fri Dec 16 07:54:04 2011 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Dec 16 07:54:10 2011 Subject: [Histonet] HT Exam In-Reply-To: <4DFB5C0F33EA40178F326A1B90929D5D@JoannePC> References: <4DFB5C0F33EA40178F326A1B90929D5D@JoannePC> Message-ID: Congrats!! I still remember that day, I cried and my husband thought I was crying because I didn't pass when I was crying from pure joy and relief! Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Thursday, December 15, 2011 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Exam YES!!!!! I passed . . . This has been a most wonderful day. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From NMP <@t> stowers.org Fri Dec 16 08:35:17 2011 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Fri Dec 16 08:35:28 2011 Subject: [Histonet] HT Exam In-Reply-To: References: <4DFB5C0F33EA40178F326A1B90929D5D@JoannePC> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF993A8AC63@EXCHMB-02.stowers-institute.org> I know exactly what you mean and how you felt :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, December 16, 2011 7:54 AM To: Joanne Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] HT Exam Congrats!! I still remember that day, I cried and my husband thought I was crying because I didn't pass when I was crying from pure joy and relief! Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Thursday, December 15, 2011 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Exam YES!!!!! I passed . . . This has been a most wonderful day. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From an.eerdekens <@t> uzleuven.be Fri Dec 16 08:53:05 2011 From: an.eerdekens <@t> uzleuven.be (An Eerdekens) Date: Fri Dec 16 08:53:13 2011 Subject: [Histonet] unsubscribe Message-ID: <09F2BA9A1E06C64D9F090FF0FD0D7317A9DB4FB875@EX2007-MBX-2.uz.kuleuven.ac.be> Dear, I want to unsubscribe from the list of histonet. Regards dr. An Eerdekens kinderarts-neonatoloog an.eerdekens@uzleuven.be tel. +32 16 34 1825 tel. secr. +32 16 34 32 11 UZ Leuven | campus Gasthuisberg | Herestraat 49 | B - 3000 Leuven (Belgium) | www.uzleuven.be From kaitlin <@t> prometheushealthcare.com Fri Dec 16 10:24:10 2011 From: kaitlin <@t> prometheushealthcare.com (Kaitlin Webster) Date: Fri Dec 16 10:24:07 2011 Subject: [Histonet] NYC Opening Message-ID: <004b01ccbc0f$247042d0$6d50c870$@prometheushealthcare.com> Top hospital in NYC seeking a Histotech with 2-5 years of recent experience in hospital. National certification required. Will assist with relocation and state licensing. For more information, please contact me at Kaitlin@prometheushealthcare.com or 407.334.4438. From talulahgosh <@t> gmail.com Fri Dec 16 11:06:49 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Dec 16 11:06:55 2011 Subject: [Histonet] unsubscribe In-Reply-To: <09F2BA9A1E06C64D9F090FF0FD0D7317A9DB4FB875@EX2007-MBX-2.uz.kuleuven.ac.be> References: <09F2BA9A1E06C64D9F090FF0FD0D7317A9DB4FB875@EX2007-MBX-2.uz.kuleuven.ac.be> Message-ID: As much as I love your endearment, we hardly know each other! Link to unsubcribe is at the bottom of histonet emails The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Fri, Dec 16, 2011 at 9:53 AM, An Eerdekens wrote: > > Dear, > > I want to unsubscribe from the list of histonet. > > Regards > dr. An Eerdekens > kinderarts-neonatoloog > > an.eerdekens@uzleuven.be > tel. +32 16 34 1825 > tel. secr. +32 16 34 32 11 > UZ Leuven | campus Gasthuisberg | Herestraat 49 | B - 3000 Leuven > (Belgium) | www.uzleuven.be > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From awatanabe <@t> tgen.org Fri Dec 16 12:12:01 2011 From: awatanabe <@t> tgen.org (awatanabe@tgen.org) Date: Fri Dec 16 12:12:05 2011 Subject: [Histonet] 2% low melting agarose gel In-Reply-To: <20111216180511.D9EBFD4C071@mr3.tgen.org> Message-ID: I have had much trouble with the world of agarose and cell lines. I finally gave up on low melt agarose and went with the standard DNA grade agarose. I also only make it 0.8% and once I fixed the processor routine and a few minor details I've had much better success. I would be happy to discuss with you in detail. Just let me know. Aprill Watanabe, B.S. Research Associate II Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Macromolecular Analyte Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Main: 602-343-8822 Fax: 602-343-8717 Cell: 602-481-8654 email: awatanabe@tgen.org website: www.tgen.org On 12/16/11 11:05 AM, "histonet-request@lists.utsouthwestern.edu" wrote: >2% low melting agarose gel From talulahgosh <@t> gmail.com Fri Dec 16 12:15:54 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Dec 16 12:15:59 2011 Subject: [Histonet] 2% low melting agarose gel In-Reply-To: References: <20111216180511.D9EBFD4C071@mr3.tgen.org> Message-ID: How is low melting point different than DNA grade? I assume it's cheaper, anything else? I know I could google it, but it's more fun to ask you guys. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Fri, Dec 16, 2011 at 1:12 PM, wrote: > I have had much trouble with the world of agarose and cell lines. I > finally gave up on low melt agarose and went with the standard DNA grade > agarose. I also only make it 0.8% and once I fixed the processor routine > and a few minor details I've had much better success. I would be happy to > discuss with you in detail. Just let me know. > > Aprill Watanabe, B.S. > Research Associate II > Integrated Cancer Genomics Division > Tissue Microarray Center (TMA) > Macromolecular Analyte Processing Center (MAPC) > Translational Genomics Research Institute (TGen) > 445 North 5th Street > Phoenix, AZ 85004 > Main: 602-343-8822 > Fax: 602-343-8717 > Cell: 602-481-8654 > email: awatanabe@tgen.org > website: www.tgen.org > > > > On 12/16/11 11:05 AM, "histonet-request@lists.utsouthwestern.edu" > wrote: > > >2% low melting agarose gel > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Ramona_Nelson <@t> bd.com Fri Dec 16 15:26:18 2011 From: Ramona_Nelson <@t> bd.com (Ramona_Nelson@bd.com) Date: Fri Dec 16 15:26:27 2011 Subject: [Histonet] AUTO: Ramona Nelson is out of the office. (returning 01/03/2012) Message-ID: I am out of the office until 01/03/20 I Note: This is an automated response to your message &quo t;Histonet Digest, Vol 97, Issue 19" PM. This is the only notification you will receive whi person is away. _________________________________________________________________ ************************************************************* ****** IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This messa services or message and you wou advertisements or solicitations from this e-mail to optoutbygroup@bd.com. * ****************************************************************** T his m the designated proprietary information an attorney-client privilege or other confidentialit If you are not a designated recipient, you may not review or distribute this message. If you received this in error, pl notify the sender by reply e-mail and delete this message. Thank you . **************************************************************** *** and Compan ******************* ************************************************ From Timothy.Morken <@t> ucsfmedctr.org Fri Dec 16 16:29:53 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Dec 16 16:30:01 2011 Subject: [Histonet] Formalin cost account Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE5CE@MCINFRWEM003.ucsfmedicalcenter.org> Sara wrote: "I don't suppose that anyone out in HistoPersonLand has done a cost comparison between making your own 10% NBF and purchasing a 50-gallon drum of 10% NBF, perhaps?" I uploaded a spreadsheet for this at my Yahoo Groups page, Histoinfo. Anyone can join the group and download the spreadsheet. Just adjust prices for your situation. There are some other histo-related downloads there as well for IHC validation. http://pets.groups.yahoo.com/group/histoinfo/ Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org From sowmyakedarnath <@t> gmail.com Fri Dec 16 18:10:16 2011 From: sowmyakedarnath <@t> gmail.com (Sowmya Kedarnath) Date: Fri Dec 16 18:10:19 2011 Subject: [Histonet] Looking for a used Biocare Decloaker Message-ID: Hello Histonetters! Anybody interested in selling a used Biocare Decloaker kindly email me with your contact details.Would greatly appreciate the help. Best Sowmya Kedarnath From akemiat3377 <@t> yahoo.com Fri Dec 16 18:19:59 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Dec 16 18:20:06 2011 Subject: [Histonet] Looking for a used Biocare Decloaker In-Reply-To: References: Message-ID: <3993039B-E202-424D-97E7-1C60B18B8A3B@yahoo.com> Why don't you ask your local Biocare rep if they have any Demo's available for sale. They used to have some available for sale. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 16, 2011, at 4:10 PM, Sowmya Kedarnath wrote: > Hello Histonetters! > Anybody interested in selling a used Biocare Decloaker kindly email me > with your contact details.Would greatly appreciate the help. > Best > Sowmya Kedarnath > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Fri Dec 16 19:56:31 2011 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri Dec 16 19:56:36 2011 Subject: [Histonet] CD31 for rat tissue Message-ID: <4EEBF6CF.3020202@umn.edu> Hello Histonetters, I am looking for a CD31 that has been proven to work well in rat tissue samples...have any of you out there done this with success? I have good human and mouse antibodies not rat! Thanks in advance! Colleen Forster Bionet Histology Research Laboratory U of MN 612-626-1930 From lucie.s.guernsey <@t> gmail.com Fri Dec 16 20:08:54 2011 From: lucie.s.guernsey <@t> gmail.com (Lucie Guernsey) Date: Fri Dec 16 20:09:40 2011 Subject: [Histonet] CD31 for rat tissue In-Reply-To: <4EEBF6CF.3020202@umn.edu> References: <4EEBF6CF.3020202@umn.edu> Message-ID: Colleen, Unfortunately, I can't help you out, but I'm also interested in this question. If you happen to get responses off-list, can you share which rat antibodies are suggested by others? Also, would you mind sharing which mouse antibody and dilution you found works well? Thanks! Lucie Lucie Guernsey UC San Diego Dept. of Pathology On Fri, Dec 16, 2011 at 5:56 PM, Colleen Forster wrote: > Hello Histonetters, > > I am looking for a CD31 that has been proven to work well in rat tissue > samples...have any of you out there done this with success? I have good > human and mouse antibodies not rat! > > Thanks in advance! > > Colleen Forster > Bionet Histology Research Laboratory > U of MN > 612-626-1930 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Diane.Tokugawa <@t> kp.org Fri Dec 16 23:44:46 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Fri Dec 16 23:44:56 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 12/16/2011 and will not return until 12/20/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Client services 8-421-5408 to reach Maria (IHC), Mario at 8-421-4961, Kiran at 8-421-5404, or Wanda at 8-421-5426. From gu.lang <@t> gmx.at Sat Dec 17 07:06:44 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Dec 17 08:06:48 2011 Subject: [Histonet] paraffin recycler Message-ID: <18AEAD4EAFD74440816FF353ED18534A@dielangs.at> Hi all! What do you think about paraffin recycler? Can someone recommend a special type or company? Do the majority use paraffindispensers or just filled containers in the heating chamber? thank you Gudrun Lang Histolab, Linz, Austria From rjbuesa <@t> yahoo.com Sat Dec 17 09:17:20 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 17 09:17:31 2011 Subject: [Histonet] paraffin recycler In-Reply-To: <18AEAD4EAFD74440816FF353ED18534A@dielangs.at> Message-ID: <1324135040.31432.YahooMailClassic@web65711.mail.ac4.yahoo.com> I always added melted paraffin from a paraffin dispenser into the hot chamber of the embedding station. It never crossed my mind to try to recycle paraffin. I strongly recommend a cost study (cost of such instrument if there exists one) against cost of new paraffin to replace "used" (reagents and tissue contaminated) paraffin. Ren? J. --- On Sat, 12/17/11, Gudrun Lang wrote: From: Gudrun Lang Subject: [Histonet] paraffin recycler To: histonet@lists.utsouthwestern.edu Date: Saturday, December 17, 2011, 8:06 AM Hi all! What do you think about paraffin recycler? Can someone recommend a special type or company? Do the majority use paraffindispensers or just filled containers in the heating chamber? thank you Gudrun Lang Histolab, Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Sat Dec 17 11:01:15 2011 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Dec 17 10:00:15 2011 Subject: [Histonet] DAB haematoxylin counterstain; too purple, overpowering IHC Message-ID: <1353970564.20111217200115@mail.ru> Megan, Your contersain DAB protocol has two weak point: 1- ammonia water. I used instead ammonia wash buffer (Tris-EDTA). It is very good and soft for IHC sections. 2- your blueing time is very short. Please try after 20-30 sec in hematoxyline dip for 1-3 mins slides into wash buffer, then finish as usual and you will see clear and crisp counterstain. If hematoxyline will be to much, then you can differentiate it in 0.25% HCl 5-10-15 dips, then wash buffer. Hope this help. Maxim Peshkov, Russia, Taganrog. > From: "Megan French" > Subject: [Histonet] DAB haematoxylin counterstain; too purple, > overpowering IHC > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi all, > I have been working through an immuno using DAB and counterstaining with > harris haematoxylin. > Protocol for counterstain: > 5 min h20 > 1 dip haematoxylin > 1 min h20 > 2 dips Blue in ammonia > 1 min h20 > Dehydrate, clear, mount > I am finding that my slides are coming out realllly purple and are > overpowering my DAB staining so much so that I can't even see it! > The haematoxylin is new, it was recently changed. I don't think it has > anything to do with the DAB/quenching etc im pretty sure is is haem > related. > Any suggestions would be appreciated!! > Megan French > Surgical Research; Murdoch Childrens Research Institute > E megan.french@mcri.edu.au mailto:Maxim_71@mail.ru From mdpraet <@t> gmail.com Sat Dec 17 10:28:54 2011 From: mdpraet <@t> gmail.com (mequita praet) Date: Sat Dec 17 10:28:56 2011 Subject: [Histonet] Re: New Lab Message-ID: Hi Phillip, I see you have gotton lots of responses. I am also available on a part-time consultant basis to help set up histology labs. I would love to talk with about this opportunity. I am sure you can continue to explore the resourse available here on histonet and work your way through this project. However, I do believe you are taking on a monmental task, why not let a histotech with experience help you with the lab set up? You really will have enough to do to handle office set up ( medicare requirements, billing, courier service, etc). The headaches will many and long. Your idea of a lab with wide open spaces and no walls demonstrates that you could use more in put from a histotech with lab set up experience. After 40 years in the histology lab, I could offer you that input. My consultantion fees are small in comparsion to the headaches. If you would like to see my resume and discuss this further please feel free to email me. Mequita Praet, HTL(ASCP)SLS mdpraet@gmail.com From madeleinehuey <@t> gmail.com Sat Dec 17 15:28:52 2011 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sat Dec 17 15:28:58 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 20 In-Reply-To: <4eecd8d1.2409ec0a.12d2.39a3SMTPIN_ADDED@mx.google.com> References: <4eecd8d1.2409ec0a.12d2.39a3SMTPIN_ADDED@mx.google.com> Message-ID: Magan, Your problem is very simple. First of all, you do not need Harris Hematoxylin after IHC, because Harris Hematoxylin is a regressive hematoxylin. What you need is a progressive hematoxylin, like Mayer, Gill (I, II, III) & etc. Try this simple procedure after your DAB chromogen; 1) Counterstain in Gill I (Sigma) or Mayer (American MasterTech) for 0.5 - 1 min (longer if want darker counterstain, or use Gill II/III. Personal preferences) 2) Wash off excess Hematoxylin with tap water (no need distilled water, your experiment is done) 3) Blue the Nuclei with PBS or TBS buffer (common buffers used by IHC) 4) Wash with water 5) Dehydrate & cover slip with permanent mounting You can write or call me if you still need help or have any questions. Madeleine Huey BS, HTL (ASCP) QIHC Supervisor-Pathology El Camino Hospital Mountain View, CA madeleine_h@elcaminohospital.org On Sat, Dec 17, 2011 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. 2% low melting agarose gel (awatanabe@tgen.org) > ? 2. Re: 2% low melting agarose gel (Emily Sours) > ? 3. AUTO: Ramona Nelson is out of the office. (returning > ? ? ?01/03/2012) (Ramona_Nelson@bd.com) > ? 4. Formalin cost account (Morken, Timothy) > ? 5. Looking for a used Biocare Decloaker (Sowmya Kedarnath) > ? 6. Re: Looking for a used Biocare Decloaker (Akemi Allison) > ? 7. CD31 for rat tissue (Colleen Forster) > ? 8. Re: CD31 for rat tissue (Lucie Guernsey) > ? 9. Diane Tokugawa/CA/KAIPERM is out of the office. > ? ? ?(Diane.Tokugawa@kp.org) > ?10. paraffin recycler (Gudrun Lang) > ?11. Re: paraffin recycler (Rene J Buesa) > ?12. Re: DAB haematoxylin counterstain; ? ? ? ?too purple, overpowering > ? ? ?IHC (Maxim Peshkov) > ?13. Re: New Lab (mequita praet) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 16 Dec 2011 18:12:01 +0000 > From: > Subject: [Histonet] 2% low melting agarose gel > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > I have had much trouble with the world of agarose and cell lines. ?I > finally gave up on low melt agarose and went with the standard DNA grade > agarose. ?I also only make it 0.8% and once I fixed the processor routine > and a few minor details I've had much better success. ?I would be happy to > discuss with you in detail. ?Just let me know. > > Aprill Watanabe, B.S. > Research Associate II > Integrated Cancer Genomics Division > Tissue Microarray Center (TMA) > Macromolecular Analyte Processing Center (MAPC) > Translational Genomics Research Institute (TGen) > 445 North 5th Street > Phoenix, AZ 85004 > Main: 602-343-8822 > Fax: 602-343-8717 > Cell: 602-481-8654 > email: awatanabe@tgen.org > website: www.tgen.org > > > > On 12/16/11 11:05 AM, "histonet-request@lists.utsouthwestern.edu" > wrote: > >>2% low melting agarose gel > > > > > ------------------------------ > > Message: 2 > Date: Fri, 16 Dec 2011 13:15:54 -0500 > From: Emily Sours > Subject: Re: [Histonet] 2% low melting agarose gel > To: awatanabe@tgen.org, histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=UTF-8 > > How is low melting point different than DNA grade? I assume it's cheaper, > anything else? > I know I could google it, but it's more fun to ask you guys. > > Emily > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that???s beautiful. > --Ron Swanson > > > > On Fri, Dec 16, 2011 at 1:12 PM, wrote: > >> I have had much trouble with the world of agarose and cell lines. ?I >> finally gave up on low melt agarose and went with the standard DNA grade >> agarose. ?I also only make it 0.8% and once I fixed the processor routine >> and a few minor details I've had much better success. ?I would be happy to >> discuss with you in detail. ?Just let me know. >> >> Aprill Watanabe, B.S. >> Research Associate II >> Integrated Cancer Genomics Division >> Tissue Microarray Center (TMA) >> Macromolecular Analyte Processing Center (MAPC) >> Translational Genomics Research Institute (TGen) >> 445 North 5th Street >> Phoenix, AZ 85004 >> Main: 602-343-8822 >> Fax: 602-343-8717 >> Cell: 602-481-8654 >> email: awatanabe@tgen.org >> website: www.tgen.org >> >> >> >> On 12/16/11 11:05 AM, "histonet-request@lists.utsouthwestern.edu" >> wrote: >> >> >2% low melting agarose gel >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ > > Message: 3 > Date: Fri, 16 Dec 2011 16:26:18 -0500 > From: Ramona_Nelson@bd.com > Subject: [Histonet] AUTO: Ramona Nelson is out of the office. > ? ? ? ?(returning ? ? ?01/03/2012) > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="US-ASCII" > > > > > > ? I am out of the office until 01/03/20 > > > I > > > > > Note: ?This ?is ?an ?automated ?response ?to ?your message &quo ? t;Histonet ?Digest, ?Vol ?97, ?Issue 19" ? PM. > > This ?is ?the ?only ?notification ?you will receive whi ? person is away. > > > ? ? _________________________________________________________________ > > > > > ************************************************************* ? ****** > IMPORTANT ?MESSAGE FOR RECIPIENTS IN THE U.S.A.: > This > ? messa ? services ?or ? ?message ?and ?you ?wou ? advertisements ?or ?solicitations ?from ? this ? ? ? ? ?e-mail ? ? ? ? to ? ? ? ? optoutbygroup@bd.com. > * ? ****************************************************************** > T > ? his ?m ? the ? designated ? proprietary ? ?information ? ?an ? attorney-client > privilege ?or other confidentialit ? If you are not a designated recipient, you may not review ? or ?distribute this message. If you received this in error, pl ? notify ?the ?sender ?by ?reply e-mail and delete this message. Thank > ? you ? . > **************************************************************** > ? *** ? and ?Compan ? ******************* ? ************************************************ > > > > ------------------------------ > > Message: 4 > Date: Fri, 16 Dec 2011 14:29:53 -0800 > From: "Morken, Timothy" > Subject: [Histonet] Formalin cost account > To: "'Histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ?<8D7C2D242DBD45498006B21122072BF89F5EE5CE@MCINFRWEM003.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=us-ascii > > > Sara wrote: > > "I don't suppose that anyone out in HistoPersonLand has done a cost > comparison between making your own 10% NBF and purchasing a 50-gallon > drum of 10% NBF, perhaps?" > > I uploaded a spreadsheet for this at my Yahoo Groups page, Histoinfo. Anyone can join the group and download the spreadsheet. Just adjust prices for your situation. > > There are some other histo-related downloads there as well for IHC validation. > > http://pets.groups.yahoo.com/group/histoinfo/ > > > Tim Morken > Supervisor, Histology, IPOX > UC San Francisco Medical Center > Box 1656 > 1600 Divisidero St, B217 > San Francisco, CA 94115 > USA > > 415.514.6042?(office) > 415.885.7409?Fax > tim.morken@ucsfmedctr.org > > > > ------------------------------ > > Message: 5 > Date: Fri, 16 Dec 2011 16:10:16 -0800 > From: Sowmya Kedarnath > Subject: [Histonet] Looking for a used Biocare Decloaker > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Hello Histonetters! > Anybody interested in selling a used Biocare Decloaker kindly email me > with your contact details.Would greatly appreciate the help. > Best > Sowmya Kedarnath > > > > ------------------------------ > > Message: 6 > Date: Fri, 16 Dec 2011 16:19:59 -0800 > From: Akemi Allison > Subject: Re: [Histonet] Looking for a used Biocare Decloaker > To: Sowmya Kedarnath > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <3993039B-E202-424D-97E7-1C60B18B8A3B@yahoo.com> > Content-Type: text/plain; ? ? ? charset=US-ASCII; ? ? ? delsp=yes; ? ? ?format=flowed > > Why don't you ask your local Biocare rep if they have any Demo's > available for sale. ?They used to have some available for sale. > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Dec 16, 2011, at 4:10 PM, Sowmya Kedarnath wrote: > >> Hello Histonetters! >> Anybody interested in selling a used Biocare Decloaker kindly email me >> with your contact details.Would greatly appreciate the help. >> Best >> Sowmya Kedarnath >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Fri, 16 Dec 2011 19:56:31 -0600 > From: Colleen Forster > Subject: [Histonet] CD31 for rat tissue > To: Histonet > Message-ID: <4EEBF6CF.3020202@umn.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Hello Histonetters, > > I am looking for a CD31 that has been proven to work well in rat tissue > samples...have any of you out there done this with success? I have good > human and mouse antibodies not rat! > > Thanks in advance! > > Colleen Forster > Bionet Histology Research Laboratory > U of MN > 612-626-1930 > > ------------------------------ > > Message: 8 > Date: Fri, 16 Dec 2011 18:08:54 -0800 > From: Lucie Guernsey > Subject: Re: [Histonet] CD31 for rat tissue > To: Colleen Forster > Cc: Histonet > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Colleen, > > Unfortunately, I can't help you out, but I'm also interested in this > question. If you happen to get responses off-list, can you share which rat > antibodies are suggested by others? Also, would you mind sharing which > mouse antibody and dilution you found works well? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > Dept. of Pathology > > > On Fri, Dec 16, 2011 at 5:56 PM, Colleen Forster wrote: > >> Hello Histonetters, >> >> I am looking for a CD31 that has been proven to work well in rat tissue >> samples...have any of you out there done this with success? I have good >> human and mouse antibodies not rat! >> >> Thanks in advance! >> >> Colleen Forster >> Bionet Histology Research Laboratory >> U of MN >> 612-626-1930 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > ------------------------------ > > Message: 9 > Date: Fri, 16 Dec 2011 21:44:46 -0800 > From: Diane.Tokugawa@kp.org > Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=US-ASCII > > > > I will be out of the office starting ?12/16/2011 and will not return until > 12/20/2011. > > Note: ? For Cytology issues, please call Molly ?at 8-421-5487, ?Eric at > 8-421-5405, or Wanda 8-421-5426 ? For Histology / IHC issues, please call > Client services 8-421-5408 to reach Maria (IHC), Mario at 8-421-4961, Kiran > at 8-421-5404, ?or Wanda at 8-421-5426. > > ------------------------------ > > Message: 10 > Date: Sat, 17 Dec 2011 14:06:44 +0100 > From: "Gudrun Lang" > Subject: [Histonet] paraffin recycler > To: > Message-ID: <18AEAD4EAFD74440816FF353ED18534A@dielangs.at> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Hi all! > > What do you think about paraffin recycler? Can someone recommend a special > type or company? > > Do the majority use paraffindispensers or just filled containers in the > heating chamber? > > > > thank you > > Gudrun Lang > > > > Histolab, Linz, Austria > > > > ------------------------------ > > Message: 11 > Date: Sat, 17 Dec 2011 07:17:20 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] paraffin recycler > To: histonet@lists.utsouthwestern.edu, gu.lang@gmx.at > Message-ID: > ? ? ? ?<1324135040.31432.YahooMailClassic@web65711.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I always added melted paraffin from a paraffin dispenser into the hot chamber of the embedding station. > It never crossed my mind to try to recycle paraffin. I strongly recommend a cost study (cost of such instrument if there exists one) against cost of new paraffin to replace "used" > (reagents and tissue contaminated) paraffin. > Ren? J. > > --- On Sat, 12/17/11, Gudrun Lang wrote: > > > From: Gudrun Lang > Subject: [Histonet] paraffin recycler > To: histonet@lists.utsouthwestern.edu > Date: Saturday, December 17, 2011, 8:06 AM > > > Hi all! > > What do you think about paraffin recycler? Can someone recommend a special > type or company? > > Do the majority use paraffindispensers or just filled containers in the > heating chamber? > > > > thank you > > Gudrun Lang > > > > Histolab, Linz, Austria > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 12 > Date: Sat, 17 Dec 2011 20:01:15 +0300 > From: Maxim Peshkov > Subject: Re: [Histonet] DAB haematoxylin counterstain; ?too purple, > ? ? ? ?overpowering IHC > To: "Megan French" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <1353970564.20111217200115@mail.ru> > Content-Type: text/plain; charset=windows-1251 > > Megan, > Your contersain DAB protocol has two weak point: > 1- ammonia water. I used instead ammonia wash buffer > (Tris-EDTA). It is very good and soft for IHC sections. > 2- your blueing time is very short. > Please try after 20-30 sec in hematoxyline dip for 1-3 > mins slides into wash buffer, then finish as usual and > you will see clear and crisp counterstain. > If hematoxyline will be to much, then you can > differentiate it in 0.25% HCl 5-10-15 dips, then wash > buffer. > Hope this help. > Maxim Peshkov, > Russia, > Taganrog. > >> From: "Megan French" >> Subject: [Histonet] DAB haematoxylin counterstain; ? too purple, >> ? ? ? ? overpowering IHC >> To: >> Message-ID: >> >> Content-Type: text/plain; ? ? ? charset="us-ascii" >> >> Hi all, >> I have been working through an immuno using DAB and counterstaining with >> harris haematoxylin. >> Protocol for counterstain: >> 5 min h20 >> 1 dip haematoxylin >> 1 min h20 >> 2 dips Blue in ammonia >> 1 min h20 >> Dehydrate, clear, mount >> I am finding that my slides are coming out realllly purple and are >> overpowering my DAB staining so much so that I can't even see it! >> The haematoxylin is new, it was recently changed. I don't think it has >> anything to do with the DAB/quenching etc im pretty sure is is haem >> related. >> Any suggestions would be appreciated!! >> Megan French >> Surgical Research; Murdoch Childrens Research Institute >> E megan.french@mcri.edu.au > > ? ? ? ? ? ? ? ? ? ? ? ? ?mailto:Maxim_71@mail.ru > > > > > ------------------------------ > > Message: 13 > Date: Sat, 17 Dec 2011 11:28:54 -0500 > From: mequita praet > Subject: [Histonet] Re: New Lab > To: histonet > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Phillip, > I see you have gotton lots of responses. I am also available on a > part-time consultant basis to help set up histology labs. I would love > to talk with about this opportunity. I am sure you can continue to > explore the resourse available here on histonet and work your way > through this project. However, I do believe you are taking on a > monmental task, why not let a histotech with experience help you with > the lab set up? You really will have enough to do to handle office set > up ( medicare requirements, billing, courier service, etc). The > headaches will many and long. > > Your idea of a lab with wide open spaces and no walls demonstrates > that you could use more in put from a histotech with lab set up > experience. After 40 years in the histology lab, I could offer you > that input. ? My consultantion fees are small in comparsion to the > headaches. > If you would like to see my resume and discuss this further please > feel free to email me. > Mequita Praet, HTL(ASCP)SLS > mdpraet@gmail.com > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 20 > **************************************** From pruegg <@t> ihctech.net Sat Dec 17 15:37:32 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Dec 17 15:37:38 2011 Subject: [Histonet] CD31 for rat tissue In-Reply-To: References: <4EEBF6CF.3020202@umn.edu> Message-ID: <6E256BE99AC343C18C708E401385D211@prueggihctechlt> Yea there is a great rat anti mouse cd31 but I do not know of one for rat either. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Friday, December 16, 2011 7:09 PM To: Colleen Forster Cc: Histonet Subject: Re: [Histonet] CD31 for rat tissue Colleen, Unfortunately, I can't help you out, but I'm also interested in this question. If you happen to get responses off-list, can you share which rat antibodies are suggested by others? Also, would you mind sharing which mouse antibody and dilution you found works well? Thanks! Lucie Lucie Guernsey UC San Diego Dept. of Pathology On Fri, Dec 16, 2011 at 5:56 PM, Colleen Forster wrote: > Hello Histonetters, > > I am looking for a CD31 that has been proven to work well in rat tissue > samples...have any of you out there done this with success? I have good > human and mouse antibodies not rat! > > Thanks in advance! > > Colleen Forster > Bionet Histology Research Laboratory > U of MN > 612-626-1930 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Dec 17 15:49:39 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Dec 17 15:49:45 2011 Subject: [Histonet] e-cadherin Message-ID: Hello Friends, I am having a problem with mouse anti e-cadherin staining, I use novacastra e-cad and have not had problems with it before on human samples with Leica Bond Refine hrp/dab detection, but what I am trying to stain now is mouse xenographs with human tumor cells in them, so for all the mouse on mouse abs on these I have been using with great success the new Thermo MOM kit which uses a rodent block and then mouse labeled polymer hrp. This has worked well for me on several mouse antibodies mostly from novacasta such as vimentin, CK AE1/AE3, beta-catenin, and many others but not the e-cadherin. We tested our human skin control with our usual Leica Refine hrp/dab detection and it stained, it was a little weak but it did stain, we ran the same tissue using the rodent block and MOM labeled polymer and it was completely negative, so the rodent block must be blocking that mouse antibody completely. If anyone has experience with doing ms e-cadherin on mouse xenographs with success could you please advise me? Happy holidays everyone, Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From gayle.callis <@t> bresnan.net Sun Dec 18 11:37:02 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sun Dec 18 11:37:31 2011 Subject: [Histonet] RE: mouse antiRat CD31 Message-ID: <000001ccbdab$a8942480$f9bc6d80$@bresnan.net> Several sources for mouse antiRat CD31 (PECAM) were found by doing a simple Google search with keywords mouse antiRat CD31 immunohistochemistry. You can buy this monoclonal from Serotec, BD Biosciences and probably eBiosciences plus other companies that specialize in rodent antibodies. As for protocol, IHC World had a mouse antiRat CD31 procedure that worked on FFPE tissue with recommended retrieval - certainly worth a try with your reagents. Be sure to check application e.g. IHC on FFPE or frozen sections for any particular company's technical data sheet before buying the antibody. They sometimes only test on frozen sections or with the formalin free Zinc Tris Buffer (Becksteads ZSF fixative) fixed tissue for paraffin sections ala BD Biosciences Pharmingen. As for working concentration, one should always do a dilution panel since your laboratory conditions and reagents will never be the same as in someone else's laboratory. We start our dilution panel at 10 ug/ml for solvent fixed fresh tissue frozen sections, and 20ug/ml for FFPE, and often fill in a wide gap if doing a serial dilution. The gap could be 1:500 then 1:1000, so we toss in a 1:750, and sometimes a 1:1500. Gayle M. Callis HTL/HT/MT(ASCP) From madeleinehuey <@t> gmail.com Sun Dec 18 22:18:51 2011 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sun Dec 18 22:18:57 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 21 In-Reply-To: <4eee2a48.43bfec0a.6e49.5c13SMTPIN_ADDED@mx.google.com> References: <4eee2a48.43bfec0a.6e49.5c13SMTPIN_ADDED@mx.google.com> Message-ID: Colleen, Many years ago I used Pharmingen's anti-Rat CD31, clone TLD-3A12 (I think, you need to check it out if they still sell it). This antibody is not recommend for formalin fixed paraffin tissues, but it will work. Following steps are critical; 1) Do not use HIER, use enzymatic digestion (need optimization with Trypsin or Proteinase K @ 37c. Lot to Lot variation from manufacture) 2) Overnight Incubation @ RT, not 4C (need warmer & long time) 3) Do not over dilute the 1st ab (ie. 1:10 - 1:25) 4) Try use a more sensitive detection system (Leica Refine DAB system) Good Luck! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor-Pathology, IPOX & Histology El Camino Hospital madeleine_h@elcaminohospital.org On Sun, Dec 18, 2011 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Re: Histonet Digest, Vol 97, Issue 20 (Madeleine Huey) > ? 2. RE: CD31 for rat tissue (Patsy Ruegg) > ? 3. e-cadherin (Patsy Ruegg) > ? 4. RE: ? mouse antiRat CD31 (gayle callis) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 17 Dec 2011 13:28:52 -0800 > From: Madeleine Huey > Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 20 > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=UTF-8 > > Magan, > > Your problem is very simple. ?First of all, you do not need Harris > Hematoxylin after IHC, because Harris Hematoxylin is a regressive > hematoxylin. ?What you need is a progressive hematoxylin, like Mayer, > Gill (I, II, III) & etc. > > Try this simple procedure after your DAB chromogen; > 1) Counterstain in Gill I (Sigma) or Mayer (American MasterTech) for > 0.5 - 1 min (longer if want darker counterstain, or use Gill II/III. > Personal preferences) > 2) Wash off excess Hematoxylin with tap water (no need distilled > water, your experiment is done) > 3) Blue the Nuclei with PBS or TBS buffer (common buffers used by IHC) > 4) Wash with water > 5) Dehydrate & cover slip with permanent mounting > > You can write or call me if you still need help or have any questions. > > Madeleine Huey BS, HTL (ASCP) QIHC > Supervisor-Pathology > El Camino Hospital > Mountain View, CA > madeleine_h@elcaminohospital.org > > On Sat, Dec 17, 2011 at 10:00 AM, > wrote: >> Send Histonet mailing list submissions to >> ? ? ? ?histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> ? ? ? ?histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> ? ? ? ?histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> ? 1. 2% low melting agarose gel (awatanabe@tgen.org) >> ? 2. Re: 2% low melting agarose gel (Emily Sours) >> ? 3. AUTO: Ramona Nelson is out of the office. (returning >> ? ? ?01/03/2012) (Ramona_Nelson@bd.com) >> ? 4. Formalin cost account (Morken, Timothy) >> ? 5. Looking for a used Biocare Decloaker (Sowmya Kedarnath) >> ? 6. Re: Looking for a used Biocare Decloaker (Akemi Allison) >> ? 7. CD31 for rat tissue (Colleen Forster) >> ? 8. Re: CD31 for rat tissue (Lucie Guernsey) >> ? 9. Diane Tokugawa/CA/KAIPERM is out of the office. >> ? ? ?(Diane.Tokugawa@kp.org) >> ?10. paraffin recycler (Gudrun Lang) >> ?11. Re: paraffin recycler (Rene J Buesa) >> ?12. Re: DAB haematoxylin counterstain; ? ? ? ?too purple, overpowering >> ? ? ?IHC (Maxim Peshkov) >> ?13. Re: New Lab (mequita praet) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Fri, 16 Dec 2011 18:12:01 +0000 >> From: >> Subject: [Histonet] 2% low melting agarose gel >> To: >> Message-ID: >> Content-Type: text/plain; charset="us-ascii" >> >> I have had much trouble with the world of agarose and cell lines. ?I >> finally gave up on low melt agarose and went with the standard DNA grade >> agarose. ?I also only make it 0.8% and once I fixed the processor routine >> and a few minor details I've had much better success. ?I would be happy to >> discuss with you in detail. ?Just let me know. >> >> Aprill Watanabe, B.S. >> Research Associate II >> Integrated Cancer Genomics Division >> Tissue Microarray Center (TMA) >> Macromolecular Analyte Processing Center (MAPC) >> Translational Genomics Research Institute (TGen) >> 445 North 5th Street >> Phoenix, AZ 85004 >> Main: 602-343-8822 >> Fax: 602-343-8717 >> Cell: 602-481-8654 >> email: awatanabe@tgen.org >> website: www.tgen.org >> >> >> >> On 12/16/11 11:05 AM, "histonet-request@lists.utsouthwestern.edu" >> wrote: >> >>>2% low melting agarose gel >> >> >> >> >> ------------------------------ >> >> Message: 2 >> Date: Fri, 16 Dec 2011 13:15:54 -0500 >> From: Emily Sours >> Subject: Re: [Histonet] 2% low melting agarose gel >> To: awatanabe@tgen.org, histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset=UTF-8 >> >> How is low melting point different than DNA grade? I assume it's cheaper, >> anything else? >> I know I could google it, but it's more fun to ask you guys. >> >> Emily >> >> The whole point of this country is if you want to eat garbage, balloon up >> to 600 pounds and die of a heart attack at 43, you can! You are free to do >> so. To me, that???s beautiful. >> --Ron Swanson >> >> >> >> On Fri, Dec 16, 2011 at 1:12 PM, wrote: >> >>> I have had much trouble with the world of agarose and cell lines. ?I >>> finally gave up on low melt agarose and went with the standard DNA grade >>> agarose. ?I also only make it 0.8% and once I fixed the processor routine >>> and a few minor details I've had much better success. ?I would be happy to >>> discuss with you in detail. ?Just let me know. >>> >>> Aprill Watanabe, B.S. >>> Research Associate II >>> Integrated Cancer Genomics Division >>> Tissue Microarray Center (TMA) >>> Macromolecular Analyte Processing Center (MAPC) >>> Translational Genomics Research Institute (TGen) >>> 445 North 5th Street >>> Phoenix, AZ 85004 >>> Main: 602-343-8822 >>> Fax: 602-343-8717 >>> Cell: 602-481-8654 >>> email: awatanabe@tgen.org >>> website: www.tgen.org >>> >>> >>> >>> On 12/16/11 11:05 AM, "histonet-request@lists.utsouthwestern.edu" >>> wrote: >>> >>> >2% low melting agarose gel >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> ------------------------------ >> >> Message: 3 >> Date: Fri, 16 Dec 2011 16:26:18 -0500 >> From: Ramona_Nelson@bd.com >> Subject: [Histonet] AUTO: Ramona Nelson is out of the office. >> ? ? ? ?(returning ? ? ?01/03/2012) >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset="US-ASCII" >> >> >> >> >> >> ? I am out of the office until 01/03/20 >> >> >> I >> >> >> >> >> Note: ?This ?is ?an ?automated ?response ?to ?your message &quo ? t;Histonet ?Digest, ?Vol ?97, ?Issue 19" ? PM. >> >> This ?is ?the ?only ?notification ?you will receive whi ? person is away. >> >> >> ? ? _________________________________________________________________ >> >> >> >> >> ************************************************************* ? ****** >> IMPORTANT ?MESSAGE FOR RECIPIENTS IN THE U.S.A.: >> This >> ? messa ? services ?or ? ?message ?and ?you ?wou ? advertisements ?or ?solicitations ?from ? this ? ? ? ? ?e-mail ? ? ? ? to ? ? ? ? optoutbygroup@bd.com. >> * ? ****************************************************************** >> T >> ? his ?m ? the ? designated ? proprietary ? ?information ? ?an ? attorney-client >> privilege ?or other confidentialit ? If you are not a designated recipient, you may not review ? or ?distribute this message. If you received this in error, pl ? notify ?the ?sender ?by ?reply e-mail and delete this message. Thank >> ? you ? . >> **************************************************************** >> ? *** ? and ?Compan ? ******************* ? ************************************************ >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Fri, 16 Dec 2011 14:29:53 -0800 >> From: "Morken, Timothy" >> Subject: [Histonet] Formalin cost account >> To: "'Histonet@lists.utsouthwestern.edu'" >> ? ? ? ? >> Message-ID: >> ? ? ? ?<8D7C2D242DBD45498006B21122072BF89F5EE5CE@MCINFRWEM003.ucsfmedicalcenter.org> >> >> Content-Type: text/plain; charset=us-ascii >> >> >> Sara wrote: >> >> "I don't suppose that anyone out in HistoPersonLand has done a cost >> comparison between making your own 10% NBF and purchasing a 50-gallon >> drum of 10% NBF, perhaps?" >> >> I uploaded a spreadsheet for this at my Yahoo Groups page, Histoinfo. Anyone can join the group and download the spreadsheet. Just adjust prices for your situation. >> >> There are some other histo-related downloads there as well for IHC validation. >> >> http://pets.groups.yahoo.com/group/histoinfo/ >> >> >> Tim Morken >> Supervisor, Histology, IPOX >> UC San Francisco Medical Center >> Box 1656 >> 1600 Divisidero St, B217 >> San Francisco, CA 94115 >> USA >> >> 415.514.6042?(office) >> 415.885.7409?Fax >> tim.morken@ucsfmedctr.org >> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Fri, 16 Dec 2011 16:10:16 -0800 >> From: Sowmya Kedarnath >> Subject: [Histonet] Looking for a used Biocare Decloaker >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hello Histonetters! >> Anybody interested in selling a used Biocare Decloaker kindly email me >> with your contact details.Would greatly appreciate the help. >> Best >> Sowmya Kedarnath >> >> >> >> ------------------------------ >> >> Message: 6 >> Date: Fri, 16 Dec 2011 16:19:59 -0800 >> From: Akemi Allison >> Subject: Re: [Histonet] Looking for a used Biocare Decloaker >> To: Sowmya Kedarnath >> Cc: histonet@lists.utsouthwestern.edu >> Message-ID: <3993039B-E202-424D-97E7-1C60B18B8A3B@yahoo.com> >> Content-Type: text/plain; ? ? ? charset=US-ASCII; ? ? ? delsp=yes; ? ? ?format=flowed >> >> Why don't you ask your local Biocare rep if they have any Demo's >> available for sale. ?They used to have some available for sale. >> >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> >> On Dec 16, 2011, at 4:10 PM, Sowmya Kedarnath wrote: >> >>> Hello Histonetters! >>> Anybody interested in selling a used Biocare Decloaker kindly email me >>> with your contact details.Would greatly appreciate the help. >>> Best >>> Sowmya Kedarnath >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 7 >> Date: Fri, 16 Dec 2011 19:56:31 -0600 >> From: Colleen Forster >> Subject: [Histonet] CD31 for rat tissue >> To: Histonet >> Message-ID: <4EEBF6CF.3020202@umn.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hello Histonetters, >> >> I am looking for a CD31 that has been proven to work well in rat tissue >> samples...have any of you out there done this with success? I have good >> human and mouse antibodies not rat! >> >> Thanks in advance! >> >> Colleen Forster >> Bionet Histology Research Laboratory >> U of MN >> 612-626-1930 >> >> ------------------------------ >> >> Message: 8 >> Date: Fri, 16 Dec 2011 18:08:54 -0800 >> From: Lucie Guernsey >> Subject: Re: [Histonet] CD31 for rat tissue >> To: Colleen Forster >> Cc: Histonet >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Colleen, >> >> Unfortunately, I can't help you out, but I'm also interested in this >> question. If you happen to get responses off-list, can you share which rat >> antibodies are suggested by others? Also, would you mind sharing which >> mouse antibody and dilution you found works well? >> >> Thanks! >> Lucie >> >> Lucie Guernsey >> UC San Diego >> Dept. of Pathology >> >> >> On Fri, Dec 16, 2011 at 5:56 PM, Colleen Forster wrote: >> >>> Hello Histonetters, >>> >>> I am looking for a CD31 that has been proven to work well in rat tissue >>> samples...have any of you out there done this with success? I have good >>> human and mouse antibodies not rat! >>> >>> Thanks in advance! >>> >>> Colleen Forster >>> Bionet Histology Research Laboratory >>> U of MN >>> 612-626-1930 >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> >> >> ------------------------------ >> >> Message: 9 >> Date: Fri, 16 Dec 2011 21:44:46 -0800 >> From: Diane.Tokugawa@kp.org >> Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset=US-ASCII >> >> >> >> I will be out of the office starting ?12/16/2011 and will not return until >> 12/20/2011. >> >> Note: ? For Cytology issues, please call Molly ?at 8-421-5487, ?Eric at >> 8-421-5405, or Wanda 8-421-5426 ? For Histology / IHC issues, please call >> Client services 8-421-5408 to reach Maria (IHC), Mario at 8-421-4961, Kiran >> at 8-421-5404, ?or Wanda at 8-421-5426. >> >> ------------------------------ >> >> Message: 10 >> Date: Sat, 17 Dec 2011 14:06:44 +0100 >> From: "Gudrun Lang" >> Subject: [Histonet] paraffin recycler >> To: >> Message-ID: <18AEAD4EAFD74440816FF353ED18534A@dielangs.at> >> Content-Type: text/plain; ? ? ? charset="us-ascii" >> >> Hi all! >> >> What do you think about paraffin recycler? Can someone recommend a special >> type or company? >> >> Do the majority use paraffindispensers or just filled containers in the >> heating chamber? >> >> >> >> thank you >> >> Gudrun Lang >> >> >> >> Histolab, Linz, Austria >> >> >> >> ------------------------------ >> >> Message: 11 >> Date: Sat, 17 Dec 2011 07:17:20 -0800 (PST) >> From: Rene J Buesa >> Subject: Re: [Histonet] paraffin recycler >> To: histonet@lists.utsouthwestern.edu, gu.lang@gmx.at >> Message-ID: >> ? ? ? ?<1324135040.31432.YahooMailClassic@web65711.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> I always added melted paraffin from a paraffin dispenser into the hot chamber of the embedding station. >> It never crossed my mind to try to recycle paraffin. I strongly recommend a cost study (cost of such instrument if there exists one) against cost of new paraffin to replace "used" >> (reagents and tissue contaminated) paraffin. >> Ren? J. >> >> --- On Sat, 12/17/11, Gudrun Lang wrote: >> >> >> From: Gudrun Lang >> Subject: [Histonet] paraffin recycler >> To: histonet@lists.utsouthwestern.edu >> Date: Saturday, December 17, 2011, 8:06 AM >> >> >> Hi all! >> >> What do you think about paraffin recycler? Can someone recommend a special >> type or company? >> >> Do the majority use paraffindispensers or just filled containers in the >> heating chamber? >> >> >> >> thank you >> >> Gudrun Lang >> >> >> >> Histolab, Linz, Austria >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 12 >> Date: Sat, 17 Dec 2011 20:01:15 +0300 >> From: Maxim Peshkov >> Subject: Re: [Histonet] DAB haematoxylin counterstain; ?too purple, >> ? ? ? ?overpowering IHC >> To: "Megan French" >> Cc: histonet@lists.utsouthwestern.edu >> Message-ID: <1353970564.20111217200115@mail.ru> >> Content-Type: text/plain; charset=windows-1251 >> >> Megan, >> Your contersain DAB protocol has two weak point: >> 1- ammonia water. I used instead ammonia wash buffer >> (Tris-EDTA). It is very good and soft for IHC sections. >> 2- your blueing time is very short. >> Please try after 20-30 sec in hematoxyline dip for 1-3 >> mins slides into wash buffer, then finish as usual and >> you will see clear and crisp counterstain. >> If hematoxyline will be to much, then you can >> differentiate it in 0.25% HCl 5-10-15 dips, then wash >> buffer. >> Hope this help. >> Maxim Peshkov, >> Russia, >> Taganrog. >> >>> From: "Megan French" >>> Subject: [Histonet] DAB haematoxylin counterstain; ? too purple, >>> ? ? ? ? overpowering IHC >>> To: >>> Message-ID: >>> >>> Content-Type: text/plain; ? ? ? charset="us-ascii" >>> >>> Hi all, >>> I have been working through an immuno using DAB and counterstaining with >>> harris haematoxylin. >>> Protocol for counterstain: >>> 5 min h20 >>> 1 dip haematoxylin >>> 1 min h20 >>> 2 dips Blue in ammonia >>> 1 min h20 >>> Dehydrate, clear, mount >>> I am finding that my slides are coming out realllly purple and are >>> overpowering my DAB staining so much so that I can't even see it! >>> The haematoxylin is new, it was recently changed. I don't think it has >>> anything to do with the DAB/quenching etc im pretty sure is is haem >>> related. >>> Any suggestions would be appreciated!! >>> Megan French >>> Surgical Research; Murdoch Childrens Research Institute >>> E megan.french@mcri.edu.au >> >> ? ? ? ? ? ? ? ? ? ? ? ? ?mailto:Maxim_71@mail.ru >> >> >> >> >> ------------------------------ >> >> Message: 13 >> Date: Sat, 17 Dec 2011 11:28:54 -0500 >> From: mequita praet >> Subject: [Histonet] Re: New Lab >> To: histonet >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hi Phillip, >> I see you have gotton lots of responses. I am also available on a >> part-time consultant basis to help set up histology labs. I would love >> to talk with about this opportunity. I am sure you can continue to >> explore the resourse available here on histonet and work your way >> through this project. However, I do believe you are taking on a >> monmental task, why not let a histotech with experience help you with >> the lab set up? You really will have enough to do to handle office set >> up ( medicare requirements, billing, courier service, etc). The >> headaches will many and long. >> >> Your idea of a lab with wide open spaces and no walls demonstrates >> that you could use more in put from a histotech with lab set up >> experience. After 40 years in the histology lab, I could offer you >> that input. ? My consultantion fees are small in comparsion to the >> headaches. >> If you would like to see my resume and discuss this further please >> feel free to email me. >> Mequita Praet, HTL(ASCP)SLS >> mdpraet@gmail.com >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 97, Issue 20 >> **************************************** > > > > ------------------------------ > > Message: 2 > Date: Sat, 17 Dec 2011 14:37:32 -0700 > From: "Patsy Ruegg" > Subject: RE: [Histonet] CD31 for rat tissue > To: "'Lucie Guernsey'" , ? ?"'Colleen > ? ? ? ?Forster'" > Cc: 'Histonet' > Message-ID: <6E256BE99AC343C18C708E401385D211@prueggihctechlt> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Yea there is a great rat anti mouse cd31 but I do not know of one for rat > either. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie > Guernsey > Sent: Friday, December 16, 2011 7:09 PM > To: Colleen Forster > Cc: Histonet > Subject: Re: [Histonet] CD31 for rat tissue > > Colleen, > > Unfortunately, I can't help you out, but I'm also interested in this > question. If you happen to get responses off-list, can you share which rat > antibodies are suggested by others? Also, would you mind sharing which > mouse antibody and dilution you found works well? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > Dept. of Pathology > > > On Fri, Dec 16, 2011 at 5:56 PM, Colleen Forster wrote: > >> Hello Histonetters, >> >> I am looking for a CD31 that has been proven to work well in rat tissue >> samples...have any of you out there done this with success? I have good >> human and mouse antibodies not rat! >> >> Thanks in advance! >> >> Colleen Forster >> Bionet Histology Research Laboratory >> U of MN >> 612-626-1930 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 3 > Date: Sat, 17 Dec 2011 14:49:39 -0700 > From: "Patsy Ruegg" > Subject: [Histonet] e-cadherin > To: "'Histonet'" > Message-ID: > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Hello Friends, > > > > I am having a problem with mouse anti e-cadherin staining, I use novacastra > e-cad and have not had problems with it before on human samples with Leica > Bond Refine hrp/dab detection, but what I am trying to stain now is mouse > xenographs with human tumor cells in them, so for all the mouse on mouse abs > on these I have been using with great success the new Thermo MOM kit which > uses a rodent block and then mouse labeled polymer hrp. ?This has worked > well for me on several mouse antibodies mostly from novacasta such as > vimentin, CK AE1/AE3, beta-catenin, and many others but not the e-cadherin. > We tested our human skin control with our usual Leica Refine hrp/dab > detection and it stained, it was a little weak but it did stain, we ran the > same tissue using the rodent block and MOM labeled polymer and it was > completely negative, so the rodent block must be blocking that mouse > antibody completely. ?If anyone has experience with doing ms e-cadherin on > mouse xenographs with success could you please advise me? > > > > Happy holidays everyone, > > > > Cheers, > > Patsy > > > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech > > 12635 Montview Blvd. Ste.215 > > Aurora, CO 80045 > > 720-859-4060 > > fax 720-859-4110 > > www.ihctech.net > > www.ihcrg.org > > > > > > ------------------------------ > > Message: 4 > Date: Sun, 18 Dec 2011 10:37:02 -0700 > From: "gayle callis" > Subject: [Histonet] RE: ? mouse antiRat CD31 > To: > Message-ID: <000001ccbdab$a8942480$f9bc6d80$@bresnan.net> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > Several sources for mouse antiRat CD31 (PECAM) were found by doing a simple > Google search with keywords mouse antiRat CD31 immunohistochemistry. ?You > can buy this monoclonal from Serotec, BD Biosciences and probably > eBiosciences plus other companies that specialize in rodent antibodies. ? As > for protocol, IHC World had a mouse antiRat CD31 procedure that worked on > FFPE tissue with recommended retrieval - certainly worth a ?try with your > reagents. ? ?Be sure to check application e.g. ?IHC on FFPE or frozen > sections for any particular company's technical data sheet before buying the > antibody. ? They sometimes only test on frozen sections or with the formalin > free Zinc Tris Buffer (Becksteads ?ZSF fixative) fixed tissue for paraffin > sections ala BD Biosciences Pharmingen. > > > > As for working concentration, one should always do a dilution panel since > your laboratory conditions and reagents will never be the same as in someone > else's laboratory. ? We start our dilution panel at 10 ug/ml for solvent > fixed fresh tissue frozen sections, and 20ug/ml for FFPE, and often fill in > a wide gap if doing a serial dilution. ? The gap could be 1:500 then 1:1000, > so we toss in a 1:750, and sometimes a 1:1500. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 21 > **************************************** From sbreeden <@t> nmda.nmsu.edu Mon Dec 19 07:04:17 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Dec 19 07:04:26 2011 Subject: [Histonet] Stain & Dye Stability Article Message-ID: <02C099024072804EA34F5906BAC30A41062C73@nmdamailsvr.nmda.ad.nmsu.edu> I've received permission to send a copy of the article from the Biological Stain Commission on Stain & Dye Stability to those who asked. If you are one of those several individuals who asked me to send you a copy, please contact me directly and I'll attach and send to you ASAP. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From ASelf <@t> georgetownhospitalsystem.org Mon Dec 19 08:38:41 2011 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Mon Dec 19 08:38:52 2011 Subject: [Histonet] Raymond Lamb Microwriter I Message-ID: Good Morning, I am looking for some help from someone that has used the Raymond Lamb Microwriter I cassette writer. We currently have one (and yes we desperately need to upgrade) that gives us trouble daily. The cassettes do not want to release from the plastic tubing into the cassette holding arm. Then there are times the arm will continuously open and close and never drop a cassette down. If there is anyone out there that has this type cassette writer that could possibly help me out please contact me. Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From dellav <@t> musc.edu Mon Dec 19 09:23:40 2011 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Dec 19 09:29:20 2011 Subject: [Histonet] Has anyone else noticed In-Reply-To: <3993039B-E202-424D-97E7-1C60B18B8A3B@yahoo.com> References: <3993039B-E202-424D-97E7-1C60B18B8A3B@yahoo.com> Message-ID: Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 From gayle.callis <@t> bresnan.net Mon Dec 19 09:40:26 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Dec 19 09:41:03 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 21 In-Reply-To: References: <4eee2a48.43bfec0a.6e49.5c13SMTPIN_ADDED@mx.google.com> Message-ID: <001201ccbe64$89119660$9b34c320$@bresnan.net> This same clone for Rat CD31 still sold by BD Biosciences/Pharmingen/Invitrogen, also Serotec, ABCAM and a company called Research Diagnostics found in the IHC World protocol. The latter protocol used Proteinase K digestion. Gayle Callis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey Sent: Sunday, December 18, 2011 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 21 Colleen, Many years ago I used Pharmingen's anti-Rat CD31, clone TLD-3A12 (I think, you need to check it out if they still sell it). This antibody is not recommend for formalin fixed paraffin tissues, but it will work. Following steps are critical; 1) Do not use HIER, use enzymatic digestion (need optimization with Trypsin or Proteinase K @ 37c. Lot to Lot variation from manufacture) 2) Overnight Incubation @ RT, not 4C (need warmer & long time) 3) Do not over dilute the 1st ab (ie. 1:10 - 1:25) 4) Try use a more sensitive detection system (Leica Refine DAB system) Good Luck! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor-Pathology, IPOX & Histology El Camino Hospital madeleine_h@elcaminohospital.org On Sun, Dec 18, 2011 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Histonet Digest, Vol 97, Issue 20 (Madeleine Huey) > 2. RE: CD31 for rat tissue (Patsy Ruegg) > 3. e-cadherin (Patsy Ruegg) > 4. RE: mouse antiRat CD31 (gayle callis) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 17 Dec 2011 13:28:52 -0800 > From: Madeleine Huey > Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 20 > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=UTF-8 > > Magan, > > Your problem is very simple. First of all, you do not need Harris > Hematoxylin after IHC, because Harris Hematoxylin is a regressive > hematoxylin. What you need is a progressive hematoxylin, like Mayer, > Gill (I, II, III) & etc. > > Try this simple procedure after your DAB chromogen; > 1) Counterstain in Gill I (Sigma) or Mayer (American MasterTech) for > 0.5 - 1 min (longer if want darker counterstain, or use Gill II/III. > Personal preferences) > 2) Wash off excess Hematoxylin with tap water (no need distilled > water, your experiment is done) > 3) Blue the Nuclei with PBS or TBS buffer (common buffers used by IHC) > 4) Wash with water > 5) Dehydrate & cover slip with permanent mounting > > You can write or call me if you still need help or have any questions. > > Madeleine Huey BS, HTL (ASCP) QIHC > Supervisor-Pathology > El Camino Hospital > Mountain View, CA > madeleine_h@elcaminohospital.org > > On Sat, Dec 17, 2011 at 10:00 AM, > wrote: >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. 2% low melting agarose gel (awatanabe@tgen.org) >> 2. Re: 2% low melting agarose gel (Emily Sours) >> 3. AUTO: Ramona Nelson is out of the office. (returning >> 01/03/2012) (Ramona_Nelson@bd.com) >> 4. Formalin cost account (Morken, Timothy) >> 5. Looking for a used Biocare Decloaker (Sowmya Kedarnath) >> 6. Re: Looking for a used Biocare Decloaker (Akemi Allison) >> 7. CD31 for rat tissue (Colleen Forster) >> 8. Re: CD31 for rat tissue (Lucie Guernsey) >> 9. Diane Tokugawa/CA/KAIPERM is out of the office. >> (Diane.Tokugawa@kp.org) >> 10. paraffin recycler (Gudrun Lang) >> 11. Re: paraffin recycler (Rene J Buesa) >> 12. Re: DAB haematoxylin counterstain; too purple, >> overpowering >> IHC (Maxim Peshkov) >> 13. Re: New Lab (mequita praet) >> >> >> --------------------------------------------------------------------- >> - >> >> Message: 1 >> Date: Fri, 16 Dec 2011 18:12:01 +0000 >> From: >> Subject: [Histonet] 2% low melting agarose gel >> To: >> Message-ID: >> Content-Type: text/plain; charset="us-ascii" >> >> I have had much trouble with the world of agarose and cell lines. I >> finally gave up on low melt agarose and went with the standard DNA >> grade agarose. I also only make it 0.8% and once I fixed the >> processor routine and a few minor details I've had much better >> success. I would be happy to discuss with you in detail. Just let me know. >> >> Aprill Watanabe, B.S. >> Research Associate II >> Integrated Cancer Genomics Division >> Tissue Microarray Center (TMA) >> Macromolecular Analyte Processing Center (MAPC) Translational >> Genomics Research Institute (TGen) >> 445 North 5th Street >> Phoenix, AZ 85004 >> Main: 602-343-8822 >> Fax: 602-343-8717 >> Cell: 602-481-8654 >> email: awatanabe@tgen.org >> website: www.tgen.org >> >> >> >> On 12/16/11 11:05 AM, "histonet-request@lists.utsouthwestern.edu" >> wrote: >> >>>2% low melting agarose gel >> >> >> >> >> ------------------------------ >> >> Message: 2 >> Date: Fri, 16 Dec 2011 13:15:54 -0500 >> From: Emily Sours >> Subject: Re: [Histonet] 2% low melting agarose gel >> To: awatanabe@tgen.org, histonet@lists.utsouthwestern.edu >> Message-ID: >> >> >> Content-Type: text/plain; charset=UTF-8 >> >> How is low melting point different than DNA grade? I assume it's >> cheaper, anything else? >> I know I could google it, but it's more fun to ask you guys. >> >> Emily >> >> The whole point of this country is if you want to eat garbage, >> balloon up to 600 pounds and die of a heart attack at 43, you can! >> You are free to do so. To me, that???s beautiful. >> --Ron Swanson >> >> >> >> On Fri, Dec 16, 2011 at 1:12 PM, wrote: >> >>> I have had much trouble with the world of agarose and cell lines. I >>> finally gave up on low melt agarose and went with the standard DNA >>> grade agarose. I also only make it 0.8% and once I fixed the >>> processor routine and a few minor details I've had much better >>> success. I would be happy to discuss with you in detail. Just let me know. >>> >>> Aprill Watanabe, B.S. >>> Research Associate II >>> Integrated Cancer Genomics Division >>> Tissue Microarray Center (TMA) >>> Macromolecular Analyte Processing Center (MAPC) Translational >>> Genomics Research Institute (TGen) >>> 445 North 5th Street >>> Phoenix, AZ 85004 >>> Main: 602-343-8822 >>> Fax: 602-343-8717 >>> Cell: 602-481-8654 >>> email: awatanabe@tgen.org >>> website: www.tgen.org >>> >>> >>> >>> On 12/16/11 11:05 AM, "histonet-request@lists.utsouthwestern.edu" >>> wrote: >>> >>> >2% low melting agarose gel >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> ------------------------------ >> >> Message: 3 >> Date: Fri, 16 Dec 2011 16:26:18 -0500 >> From: Ramona_Nelson@bd.com >> Subject: [Histonet] AUTO: Ramona Nelson is out of the office. >> (returning 01/03/2012) >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> >> >> Content-Type: text/plain; charset="US-ASCII" >> >> >> >> >> >> I am out of the office until 01/03/20 >> >> >> I >> >> >> >> >> Note: This is an automated response to your message &quo t;Histonet Digest, Vol 97, Issue 19" PM. >> >> This is the only notification you will receive whi person is away. >> >> >> _________________________________________________________________ >> >> >> >> >> ************************************************************* >> ****** IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: >> This >> messa services or message and you wou advertisements or solicitations from this e-mail to optoutbygroup@bd.com. >> * >> ****************************************************************** >> T >> his m the designated proprietary information an >> attorney-client privilege or other confidentialit If you are not a >> designated recipient, you may not review or distribute this >> message. If you received this in error, pl notify the sender by >> reply e-mail and delete this message. Thank >> you . >> **************************************************************** >> *** and Compan ******************* >> ************************************************ >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Fri, 16 Dec 2011 14:29:53 -0800 >> From: "Morken, Timothy" >> Subject: [Histonet] Formalin cost account >> To: "'Histonet@lists.utsouthwestern.edu'" >> >> Message-ID: >> >> <8D7C2D242DBD45498006B21122072BF89F5EE5CE@MCINFRWEM003.ucsfmedicalcen >> ter.org> >> >> Content-Type: text/plain; charset=us-ascii >> >> >> Sara wrote: >> >> "I don't suppose that anyone out in HistoPersonLand has done a cost >> comparison between making your own 10% NBF and purchasing a 50-gallon >> drum of 10% NBF, perhaps?" >> >> I uploaded a spreadsheet for this at my Yahoo Groups page, Histoinfo. Anyone can join the group and download the spreadsheet. Just adjust prices for your situation. >> >> There are some other histo-related downloads there as well for IHC validation. >> >> http://pets.groups.yahoo.com/group/histoinfo/ >> >> >> Tim Morken >> Supervisor, Histology, IPOX >> UC San Francisco Medical Center >> Box 1656 >> 1600 Divisidero St, B217 >> San Francisco, CA 94115 >> USA >> >> 415.514.6042 (office) >> 415.885.7409 Fax >> tim.morken@ucsfmedctr.org >> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Fri, 16 Dec 2011 16:10:16 -0800 >> From: Sowmya Kedarnath >> Subject: [Histonet] Looking for a used Biocare Decloaker >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hello Histonetters! >> Anybody interested in selling a used Biocare Decloaker kindly email >> me with your contact details.Would greatly appreciate the help. >> Best >> Sowmya Kedarnath >> >> >> >> ------------------------------ >> >> Message: 6 >> Date: Fri, 16 Dec 2011 16:19:59 -0800 >> From: Akemi Allison >> Subject: Re: [Histonet] Looking for a used Biocare Decloaker >> To: Sowmya Kedarnath >> Cc: histonet@lists.utsouthwestern.edu >> Message-ID: <3993039B-E202-424D-97E7-1C60B18B8A3B@yahoo.com> >> Content-Type: text/plain; charset=US-ASCII; delsp=yes; >> format=flowed >> >> Why don't you ask your local Biocare rep if they have any Demo's >> available for sale. They used to have some available for sale. >> >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> >> On Dec 16, 2011, at 4:10 PM, Sowmya Kedarnath wrote: >> >>> Hello Histonetters! >>> Anybody interested in selling a used Biocare Decloaker kindly email >>> me with your contact details.Would greatly appreciate the help. >>> Best >>> Sowmya Kedarnath >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 7 >> Date: Fri, 16 Dec 2011 19:56:31 -0600 >> From: Colleen Forster >> Subject: [Histonet] CD31 for rat tissue >> To: Histonet >> Message-ID: <4EEBF6CF.3020202@umn.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hello Histonetters, >> >> I am looking for a CD31 that has been proven to work well in rat >> tissue samples...have any of you out there done this with success? I >> have good human and mouse antibodies not rat! >> >> Thanks in advance! >> >> Colleen Forster >> Bionet Histology Research Laboratory >> U of MN >> 612-626-1930 >> >> ------------------------------ >> >> Message: 8 >> Date: Fri, 16 Dec 2011 18:08:54 -0800 >> From: Lucie Guernsey >> Subject: Re: [Histonet] CD31 for rat tissue >> To: Colleen Forster >> Cc: Histonet >> Message-ID: >> >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Colleen, >> >> Unfortunately, I can't help you out, but I'm also interested in this >> question. If you happen to get responses off-list, can you share >> which rat antibodies are suggested by others? Also, would you mind >> sharing which mouse antibody and dilution you found works well? >> >> Thanks! >> Lucie >> >> Lucie Guernsey >> UC San Diego >> Dept. of Pathology >> >> >> On Fri, Dec 16, 2011 at 5:56 PM, Colleen Forster wrote: >> >>> Hello Histonetters, >>> >>> I am looking for a CD31 that has been proven to work well in rat >>> tissue samples...have any of you out there done this with success? I >>> have good human and mouse antibodies not rat! >>> >>> Thanks in advance! >>> >>> Colleen Forster >>> Bionet Histology Research Laboratory U of MN >>> 612-626-1930 >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> >> >> ------------------------------ >> >> Message: 9 >> Date: Fri, 16 Dec 2011 21:44:46 -0800 >> From: Diane.Tokugawa@kp.org >> Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> >> >> Content-Type: text/plain; charset=US-ASCII >> >> >> >> I will be out of the office starting 12/16/2011 and will not return >> until 12/20/2011. >> >> Note: For Cytology issues, please call Molly at 8-421-5487, Eric >> at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, >> please call Client services 8-421-5408 to reach Maria (IHC), Mario at >> 8-421-4961, Kiran at 8-421-5404, or Wanda at 8-421-5426. >> >> ------------------------------ >> >> Message: 10 >> Date: Sat, 17 Dec 2011 14:06:44 +0100 >> From: "Gudrun Lang" >> Subject: [Histonet] paraffin recycler >> To: >> Message-ID: <18AEAD4EAFD74440816FF353ED18534A@dielangs.at> >> Content-Type: text/plain; charset="us-ascii" >> >> Hi all! >> >> What do you think about paraffin recycler? Can someone recommend a >> special type or company? >> >> Do the majority use paraffindispensers or just filled containers in >> the heating chamber? >> >> >> >> thank you >> >> Gudrun Lang >> >> >> >> Histolab, Linz, Austria >> >> >> >> ------------------------------ >> >> Message: 11 >> Date: Sat, 17 Dec 2011 07:17:20 -0800 (PST) >> From: Rene J Buesa >> Subject: Re: [Histonet] paraffin recycler >> To: histonet@lists.utsouthwestern.edu, gu.lang@gmx.at >> Message-ID: >> >> <1324135040.31432.YahooMailClassic@web65711.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> I always added melted paraffin from a paraffin dispenser into the hot chamber of the embedding station. >> It never crossed my mind to try to recycle paraffin. I strongly recommend a cost study (cost of such instrument if there exists one) against cost of new paraffin to replace "used" >> (reagents and tissue contaminated) paraffin. >> Ren? J. >> >> --- On Sat, 12/17/11, Gudrun Lang wrote: >> >> >> From: Gudrun Lang >> Subject: [Histonet] paraffin recycler >> To: histonet@lists.utsouthwestern.edu >> Date: Saturday, December 17, 2011, 8:06 AM >> >> >> Hi all! >> >> What do you think about paraffin recycler? Can someone recommend a >> special type or company? >> >> Do the majority use paraffindispensers or just filled containers in >> the heating chamber? >> >> >> >> thank you >> >> Gudrun Lang >> >> >> >> Histolab, Linz, Austria >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 12 >> Date: Sat, 17 Dec 2011 20:01:15 +0300 >> From: Maxim Peshkov >> Subject: Re: [Histonet] DAB haematoxylin counterstain; too purple, >> overpowering IHC >> To: "Megan French" >> Cc: histonet@lists.utsouthwestern.edu >> Message-ID: <1353970564.20111217200115@mail.ru> >> Content-Type: text/plain; charset=windows-1251 >> >> Megan, >> Your contersain DAB protocol has two weak point: >> 1- ammonia water. I used instead ammonia wash buffer (Tris-EDTA). It >> is very good and soft for IHC sections. >> 2- your blueing time is very short. >> Please try after 20-30 sec in hematoxyline dip for 1-3 mins slides >> into wash buffer, then finish as usual and you will see clear and >> crisp counterstain. >> If hematoxyline will be to much, then you can differentiate it in >> 0.25% HCl 5-10-15 dips, then wash buffer. >> Hope this help. >> Maxim Peshkov, >> Russia, >> Taganrog. >> >>> From: "Megan French" >>> Subject: [Histonet] DAB haematoxylin counterstain; too purple, >>> overpowering IHC >>> To: >>> Message-ID: >>> >>> Content-Type: text/plain; charset="us-ascii" >>> >>> Hi all, >>> I have been working through an immuno using DAB and counterstaining >>> with harris haematoxylin. >>> Protocol for counterstain: >>> 5 min h20 >>> 1 dip haematoxylin >>> 1 min h20 >>> 2 dips Blue in ammonia >>> 1 min h20 >>> Dehydrate, clear, mount >>> I am finding that my slides are coming out realllly purple and are >>> overpowering my DAB staining so much so that I can't even see it! >>> The haematoxylin is new, it was recently changed. I don't think it >>> has anything to do with the DAB/quenching etc im pretty sure is is >>> haem related. >>> Any suggestions would be appreciated!! >>> Megan French >>> Surgical Research; Murdoch Childrens Research Institute E >>> megan.french@mcri.edu.au >> >> mailto:Maxim_71@mail.ru >> >> >> >> >> ------------------------------ >> >> Message: 13 >> Date: Sat, 17 Dec 2011 11:28:54 -0500 >> From: mequita praet >> Subject: [Histonet] Re: New Lab >> To: histonet >> Message-ID: >> >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hi Phillip, >> I see you have gotton lots of responses. I am also available on a >> part-time consultant basis to help set up histology labs. I would >> love to talk with about this opportunity. I am sure you can continue >> to explore the resourse available here on histonet and work your way >> through this project. However, I do believe you are taking on a >> monmental task, why not let a histotech with experience help you with >> the lab set up? You really will have enough to do to handle office >> set up ( medicare requirements, billing, courier service, etc). The >> headaches will many and long. >> >> Your idea of a lab with wide open spaces and no walls demonstrates >> that you could use more in put from a histotech with lab set up >> experience. After 40 years in the histology lab, I could offer you >> that input. My consultantion fees are small in comparsion to the >> headaches. >> If you would like to see my resume and discuss this further please >> feel free to email me. >> Mequita Praet, HTL(ASCP)SLS >> mdpraet@gmail.com >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 97, Issue 20 >> **************************************** > > > > ------------------------------ > > Message: 2 > Date: Sat, 17 Dec 2011 14:37:32 -0700 > From: "Patsy Ruegg" > Subject: RE: [Histonet] CD31 for rat tissue > To: "'Lucie Guernsey'" , "'Colleen > Forster'" > Cc: 'Histonet' > Message-ID: <6E256BE99AC343C18C708E401385D211@prueggihctechlt> > Content-Type: text/plain; charset="us-ascii" > > Yea there is a great rat anti mouse cd31 but I do not know of one for > rat either. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie > Guernsey > Sent: Friday, December 16, 2011 7:09 PM > To: Colleen Forster > Cc: Histonet > Subject: Re: [Histonet] CD31 for rat tissue > > Colleen, > > Unfortunately, I can't help you out, but I'm also interested in this > question. If you happen to get responses off-list, can you share which > rat antibodies are suggested by others? Also, would you mind sharing > which mouse antibody and dilution you found works well? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > Dept. of Pathology > > > On Fri, Dec 16, 2011 at 5:56 PM, Colleen Forster wrote: > >> Hello Histonetters, >> >> I am looking for a CD31 that has been proven to work well in rat >> tissue samples...have any of you out there done this with success? I >> have good human and mouse antibodies not rat! >> >> Thanks in advance! >> >> Colleen Forster >> Bionet Histology Research Laboratory >> U of MN >> 612-626-1930 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 3 > Date: Sat, 17 Dec 2011 14:49:39 -0700 > From: "Patsy Ruegg" > Subject: [Histonet] e-cadherin > To: "'Histonet'" > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hello Friends, > > > > I am having a problem with mouse anti e-cadherin staining, I use > novacastra e-cad and have not had problems with it before on human > samples with Leica Bond Refine hrp/dab detection, but what I am trying > to stain now is mouse xenographs with human tumor cells in them, so > for all the mouse on mouse abs on these I have been using with great > success the new Thermo MOM kit which uses a rodent block and then > mouse labeled polymer hrp. This has worked well for me on several > mouse antibodies mostly from novacasta such as vimentin, CK AE1/AE3, beta-catenin, and many others but not the e-cadherin. > We tested our human skin control with our usual Leica Refine hrp/dab > detection and it stained, it was a little weak but it did stain, we > ran the same tissue using the rodent block and MOM labeled polymer and > it was completely negative, so the rodent block must be blocking that > mouse antibody completely. If anyone has experience with doing ms > e-cadherin on mouse xenographs with success could you please advise me? > > > > Happy holidays everyone, > > > > Cheers, > > Patsy > > > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech > > 12635 Montview Blvd. Ste.215 > > Aurora, CO 80045 > > 720-859-4060 > > fax 720-859-4110 > > www.ihctech.net > > www.ihcrg.org > > > > > > ------------------------------ > > Message: 4 > Date: Sun, 18 Dec 2011 10:37:02 -0700 > From: "gayle callis" > Subject: [Histonet] RE: mouse antiRat CD31 > To: > Message-ID: <000001ccbdab$a8942480$f9bc6d80$@bresnan.net> > Content-Type: text/plain; charset="us-ascii" > > Several sources for mouse antiRat CD31 (PECAM) were found by doing a > simple Google search with keywords mouse antiRat CD31 > immunohistochemistry. You can buy this monoclonal from Serotec, BD > Biosciences and probably eBiosciences plus other companies that > specialize in rodent antibodies. As for protocol, IHC World had a > mouse antiRat CD31 procedure that worked on FFPE tissue with > recommended retrieval - certainly worth a try with your reagents. > Be sure to check application e.g. IHC on FFPE or frozen sections for > any particular company's technical data sheet before buying the > antibody. They sometimes only test on frozen sections or with the > formalin free Zinc Tris Buffer (Becksteads ZSF fixative) fixed tissue for paraffin sections ala BD Biosciences Pharmingen. > > > > As for working concentration, one should always do a dilution panel > since your laboratory conditions and reagents will never be the same > as in someone else's laboratory. We start our dilution panel at 10 > ug/ml for solvent fixed fresh tissue frozen sections, and 20ug/ml for > FFPE, and often fill in a wide gap if doing a serial dilution. The > gap could be 1:500 then 1:1000, so we toss in a 1:750, and sometimes a 1:1500. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 21 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Dec 19 09:40:37 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon Dec 19 09:41:11 2011 Subject: [Histonet] RE: Has anyone else noticed In-Reply-To: References: <3993039B-E202-424D-97E7-1C60B18B8A3B@yahoo.com> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4F1F9B745@EXC-MBX3.cfs.le.ac.uk> Sounds to me as if you are wasting a few too many drops there!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: 19 December 2011 15:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone else noticed Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Mon Dec 19 09:59:46 2011 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Dec 19 10:00:13 2011 Subject: FW: [Histonet] Has anyone else noticed Message-ID: ________________________________ From: Pam Barker [mailto:relia1@earthlink.net] Sent: Monday, December 19, 2011 10:57 AM To: Della Speranza, Vinnie Subject: RE: [Histonet] Has anyone else noticed hahahahaha Vinnie! A nice Cab is a great anesthetic, and your observation is a great reason to open a bottle!! Who's with me? Happy Holidays!! -----Original Message----- From: "histonet-bounces@lists.utsouthwestern.edu" Sent: 12/19/2011 10:31 AM To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Has anyone else noticed Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Dec 19 10:03:52 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Dec 19 10:03:10 2011 Subject: [Histonet] RE: Has anyone else noticed In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101A4F1F9B745@EXC-MBX3.cfs.le.ac.uk> References: <3993039B-E202-424D-97E7-1C60B18B8A3B@yahoo.com> <7722595275A4DD4FA225B92CDBF174A101A4F1F9B745@EXC-MBX3.cfs.le.ac.uk> Message-ID: <5A2BD13465E061429D6455C8D6B40E39137DA01F7E@IBMB7Exchange.digestivespecialists.com> Boy, that was my thought! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Monday, December 19, 2011 10:41 AM To: 'Della Speranza, Vinnie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Has anyone else noticed Sounds to me as if you are wasting a few too many drops there!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: 19 December 2011 15:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone else noticed Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcampbell <@t> trianglebiomedical.com Mon Dec 19 11:22:10 2011 From: dcampbell <@t> trianglebiomedical.com (Dustin Paul Campbell) Date: Mon Dec 19 11:22:20 2011 Subject: [Histonet] Raymond Lamb Microwriter I Message-ID: <30D679350711B041B577E61FCFC90B68D004B3@tbssbs.TBS.local> Amy, Unfortunately, Raymond Lamb was bought out by Thermo Fisher years ago and they no longer support existing cassette/Slide labelers. However, TBS will be happy to support you with any issues. We picked up the existing line of cassette and slide labelers from Thermo and we sell and support the product. Call the following number and ask for the service department 919-384-9393. Hope this helps, Dustin Campbell?? Project Manager - SHUR/Mark-TVT(tm) ? Triangle Biomedical Sciences, Inc. 3014 Croasdaile Drive *? Durham?? NC? 27705? *? phone 919.384.9494 ext. 188 *???? fax 919.384.9595 dcampbell@trianglebiomedical.com?*? www.trianglebiomedical.com * TBS' Comprehensive Product Catalog -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Monday, December 19, 2011 9:39 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Raymond Lamb Microwriter I Good Morning, I am looking for some help from someone that has used the Raymond Lamb Microwriter I cassette writer. We currently have one (and yes we desperately need to upgrade) that gives us trouble daily. The cassettes do not want to release from the plastic tubing into the cassette holding arm. Then there are times the arm will continuously open and close and never drop a cassette down. If there is anyone out there that has this type cassette writer that could possibly help me out please contact me. Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mamawooo <@t> hotmail.com Mon Dec 19 11:24:52 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Mon Dec 19 11:24:59 2011 Subject: [Histonet] Has anyone else noticed In-Reply-To: References: , <3993039B-E202-424D-97E7-1C60B18B8A3B@yahoo.com>, Message-ID: I have noticed it many many times. Happy Holidays Jan Mahoney, Omaha > From: dellav@musc.edu > To: histonet@lists.utsouthwestern.edu > Date: Mon, 19 Dec 2011 10:23:40 -0500 > Subject: [Histonet] Has anyone else noticed > > > Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? > > > Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. > > Vinnie Della Speranza, MS, HTL(ASCP) > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue MSC 908 > Charleston, SC 29425 > tel. 843-792-6353 > fax. 843-792-8974 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfonner <@t> labpath.com Mon Dec 19 11:25:29 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Mon Dec 19 11:31:02 2011 Subject: [Histonet] Biocare Decloaker Message-ID: <000001ccbe73$34275090$9c75f1b0$@com> Would the person asking about a used Biocare Decloaker please contact me. We have one that is like new. No longer use it since all retrieval is online with automated stainer. Would be willing to sell. Thanks, Sheila From Timothy.Morken <@t> ucsfmedctr.org Mon Dec 19 14:42:19 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Dec 19 14:42:29 2011 Subject: [Histonet] Supervisor postion at UC San Francisco Message-ID: <8D7C2D242DBD45498006B21122072BF89F5257E4@MCINFRWEM003.ucsfmedicalcenter.org> We are still looking for the right person for the Histology Supervisor postion at University of California San Francisco Medical Center. The job description is below with the careers website url. Search for "histotechnologist" to see the supervisor listing. UCSF is soley a medical institution. Pathology has 25 residents and total of 60 pathologists, including research in the medical school. We do exentive research support in the histology lab. The Histology / Immunohistochemistry supervisor oversees general histology (tissue processing, embedding, sectioning, staining), specials stains and immunohistochemistry. The position does not cover grossing/frozen sections, transcription or cytology. We work with 12 pathologists directly and many more indirectly. There are 12 histotechnologists in the lab, the majority are ASCP-certified. The case load is 30,000 per year, 130,000 blocks/year (about 500+ per day average). Immuno does over 250 slides per day, up to 350. We are undergoing a transition to fully automated instrumentation. The supervisor will be highly involved in process improvment, LEAN processes, planning implementation of a continuous processing schedule, selection of new euipment, including H&E stainers, immuno stainers, tissue processors, etc, selection and implementation of specimen tracking barcoding system. The major project in the next two years is the design and build-out of a new laboratory at a new location near the new Mission Bay hospital complex that is under construction. This is a very busy place and never a dull moment. The staff is extremely dedicated and support from the Medical Directors is excellent as well. If interested please apply at the website below and contact me with any questions at tim.morken@ucsfmedctr.org Tim Morken Supervisor, Histology/IHC (soon to be supervisor Electron Microscopy - once this postion is filled!) Histology, Immunohistochemistry Supervisor postion at University of California San Francisco Medical Center https://careers.ucsfmedicalcenter.org Job ID 2651 At the University of California San Francisco Medical Center, teamwork and continual learning have maintained our top 10 ranking as one of " America's Best Hospitals" by U.S.News & World Report. For you, UCSF Medical Center is where you will share and discover something new every day with benefits and rewards that will last a lifetime. Job Summary: Under general direction from the Medical Director, the incumbent serves as supervisor to 3 Lead Histotechnologists and 9 Histotechnologists. In addition to supervisory duties, the incumbent serves as a technical expert providing direction to staff as well as performing all technical aspects of surgical histology and immunohistochemistry procedures. As supervisor: recruits, hires, trains, completes performance evaluations, and resolves employee issues. Provides orientation, completes competency assessments, maintains staff schedules, training and compliance documentation. Implements new tests and procedures as requested by Medical Directors. Updates and maintains the lab manuals and annual reviews required for accreditation. Integrates new technologies into the laboratory to include analyzing and selecting of new equipment that meets space constraints and operational needs. Ensures that new equipment and associated validation and staff training are performed. Ensures that all equipment is well-maintained. Ensures that staff training and documentation are completed as required. Ensures that QC documentation is complete and quality standards are maintained in the laboratory. Advises and assists researchers planning research projects and determines the ability of the laboratory to accommodate research projects, updating the Medical Director as required. Other duties as assigned. Required Qualifications: College degree in a biological science, chemistry or a related field or equivalent education and work experience experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last seven years, including senior-level experience. Demonstrated high-volume, high-quality sectioning and staining skills. Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines. Excellent interpersonal and communication skills. ASCP certification: HT licensed or eligible required (candidate will be expected to obtain certification within 1 year), HTL desirable. Excellent interpersonal communication skills required. Demonstrated ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines required. Previous, recent supervisory experience in a Histology and /or Immunoperoxidase Laboratory required. Must be experienced with information technology systems and the use of hardware and software in business and laboratory settings. Must be an effective user of spreadsheets and database software. Preferred Qualifications: ASCP certification: HT or HTL-licensed strongly preferred. Previous leadership experience in a hospital laboratory. Required License/Certification: N/A From pruegg <@t> ihctech.net Mon Dec 19 15:10:33 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Dec 19 15:10:36 2011 Subject: [Histonet] used seymour IHC printer Message-ID: Does anyone have a used Seymour printer I could purchase as a back up? I think I can use my installation disc but it has one I would want that to. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From tony.henwood <@t> health.nsw.gov.au Mon Dec 19 16:04:18 2011 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Dec 19 16:04:38 2011 Subject: [Histonet] Has anyone else noticed In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A0C11B@xmdb02.nch.kids> I keep trying to verify this scientific discovery but everything is blurry by the time I get to the bottom of the bottle, Oh sorry I need to look in the glass? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, 20 December 2011 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Has anyone else noticed ________________________________ From: Pam Barker [mailto:relia1@earthlink.net] Sent: Monday, December 19, 2011 10:57 AM To: Della Speranza, Vinnie Subject: RE: [Histonet] Has anyone else noticed hahahahaha Vinnie! A nice Cab is a great anesthetic, and your observation is a great reason to open a bottle!! Who's with me? Happy Holidays!! -----Original Message----- From: "histonet-bounces@lists.utsouthwestern.edu" Sent: 12/19/2011 10:31 AM To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Has anyone else noticed Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From turkekul <@t> gmail.com Mon Dec 19 16:51:59 2011 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Mon Dec 19 16:52:03 2011 Subject: [Histonet] bacteria staining Message-ID: I would like to stain FFPE mouse intestine sections to demonstrate most of the bacteria in the normal flora of the intestine. Any suggestions for the simplest histological stain that will show most of the bacteria? Thanks, Happy holidays! Mesru mskcc.org From tony.henwood <@t> health.nsw.gov.au Mon Dec 19 17:10:45 2011 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Dec 19 17:11:00 2011 Subject: [Histonet] bacteria staining In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A0C18D@xmdb02.nch.kids> Mesru, Use 1% aqueous Giemsa, stain dehydrated sections 5-10 minutes, dehydrate rapidly (either acetone, air dry or rapid alcohol dehydation), clear and mount Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mesruh turkekul Sent: Tuesday, 20 December 2011 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bacteria staining I would like to stain FFPE mouse intestine sections to demonstrate most of the bacteria in the normal flora of the intestine. Any suggestions for the simplest histological stain that will show most of the bacteria? Thanks, Happy holidays! Mesru mskcc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From dellav <@t> musc.edu Mon Dec 19 17:11:52 2011 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Dec 19 17:11:58 2011 Subject: [Histonet] Has anyone else noticed In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A0C11B@xmdb02.nch.kids> References: <6D6BD1DE8A5571489398B392A38A715760A0C11B@xmdb02.nch.kids> Message-ID: Actually Tony, by the time you get to the bottom of the bottle, you probably no longer care what color it is ! Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Monday, December 19, 2011 5:04 PM To: Della Speranza, Vinnie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Has anyone else noticed I keep trying to verify this scientific discovery but everything is blurry by the time I get to the bottom of the bottle, Oh sorry I need to look in the glass? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, 20 December 2011 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Has anyone else noticed ________________________________ From: Pam Barker [mailto:relia1@earthlink.net] Sent: Monday, December 19, 2011 10:57 AM To: Della Speranza, Vinnie Subject: RE: [Histonet] Has anyone else noticed hahahahaha Vinnie! A nice Cab is a great anesthetic, and your observation is a great reason to open a bottle!! Who's with me? Happy Holidays!! -----Original Message----- From: "histonet-bounces@lists.utsouthwestern.edu" Sent: 12/19/2011 10:31 AM To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Has anyone else noticed Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From jnocito <@t> satx.rr.com Mon Dec 19 18:25:59 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Dec 19 18:26:21 2011 Subject: [Histonet] Has anyone else noticed In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A0C11B@xmdb02.nch.kids> References: <6D6BD1DE8A5571489398B392A38A715760A0C11B@xmdb02.nch.kids> Message-ID: <5B631BC782D14A94996949A9A8E12AF0@JoePC> I wonder if it works with Scotch. Hmmm, seems like an experiment brewing. JTT ----- Original Message ----- From: "Tony Henwood (SCHN)" To: "'Della Speranza, Vinnie'" ; Sent: Monday, December 19, 2011 4:04 PM Subject: RE: [Histonet] Has anyone else noticed I keep trying to verify this scientific discovery but everything is blurry by the time I get to the bottom of the bottle, Oh sorry I need to look in the glass? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, 20 December 2011 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Has anyone else noticed ________________________________ From: Pam Barker [mailto:relia1@earthlink.net] Sent: Monday, December 19, 2011 10:57 AM To: Della Speranza, Vinnie Subject: RE: [Histonet] Has anyone else noticed hahahahaha Vinnie! A nice Cab is a great anesthetic, and your observation is a great reason to open a bottle!! Who's with me? Happy Holidays!! -----Original Message----- From: "histonet-bounces@lists.utsouthwestern.edu" Sent: 12/19/2011 10:31 AM To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Has anyone else noticed Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mweirauch <@t> crittenton.com Tue Dec 20 12:36:28 2011 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Tue Dec 20 12:38:03 2011 Subject: [Histonet] Suncycle Technologies recycler? Message-ID: Our Cytology section head has said she has used the alcohol recycler from this company at other hospitals, I can't find a lot of information on their site. Has anyone used the alcohol recycler (listed as a gravity operated system) for their tissue processor alcohol? From Diane.Tokugawa <@t> kp.org Tue Dec 20 13:47:39 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Tue Dec 20 13:48:01 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 12/20/2011 and will not return until 12/21/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Client services 8-421-5408 to reach Maria (IHC), Mario at 8-421-4961, Kiran at 8-421-5404, or Wanda at 8-421-5426. From pruegg <@t> ihctech.net Tue Dec 20 14:14:56 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Dec 20 14:15:01 2011 Subject: [Histonet] FOXC2 ab Message-ID: Does anyone know of a FOXC2 ab that works in ffpe 3 neg human breast CA samples or in mouse xenographs with human breast tumor cells in them? We are hoping for one that reacts just with the human tumor cells and not also the normal mouse cells. We have tried 2 so far without success. Abcam AB5060 fails to stain with 3-neg breast cancer and now Abgent AP5750C fails with xenografts. I asked about this before and did not get any responses but I will try and try again. Thank you for your help in this matter, Holiday Cheer, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From darko <@t> uab.edu Tue Dec 20 14:35:38 2011 From: darko <@t> uab.edu (Dave D Kojic) Date: Tue Dec 20 14:35:47 2011 Subject: [Histonet] Help with TRAP staining protocol Message-ID: <8294479DEEC4D644A0654924168800A5115D3EBD@UABEXMBS1.ad.uab.edu> Hello All, I am about to start working on my MS thesis and working on ground section of the mandible with some implants and HA particulates , so I need some help to tap into some of your knowledge on TRAP staining for osteoclasts. I would appreciate if I can get any help with a TRAP staining protocol for the ground section of the bone. Thank you very much Dave D Kojic DDS Biomaterials Resident Department of Prosthodontics UAB School of Dentistry 614-1919 7th Ave S Birmingham, AL 35294 Phone: 205.996.5746 From christina.thurby <@t> bms.com Tue Dec 20 14:45:02 2011 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Tue Dec 20 14:45:21 2011 Subject: [Histonet] Question about Bile Stain Control tissue Message-ID: Hello All, I have a few questions about Bile Stain control tissue: First - does anyone have or know of any good sources where I can purchase a couple of positive control blocks demonstrating bile in the tissue? I have some purchased control slides - but they appear to be from 'bile sludge' prepared to block. This is a nice emerald green when stained with the Hall's method, but our pathologists would like to see positive bile demonstrated in the tissue elements. Carson's newest book has a very nice image. Next question: Carson's procedure for the Hall's bile stain describes the positive elements as a emerald green to drab olive in color. We have a case that appears to be positive but the Hall's stain is kind of a camouflage brownish/drab olive green. Is there a chance we're doing something wrong with this procedure? Our purchased 'bile sludge' control slides are a nice emerald green. We also ran the Stein's method - this was completely negative (even on the purchased controls). Any thoughts and/or feedback is appreciated! Thanks! Kristie Christina Thurby, MLT(ASCP), HT, QIHC Research Scientist I Bristol Myers Squibb 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From Sruby <@t> 4path.com Tue Dec 20 15:08:58 2011 From: Sruby <@t> 4path.com (Stephen G. Ruby) Date: Tue Dec 20 15:09:04 2011 Subject: [Histonet] Part time hitotech positions Message-ID: Seeking part-time histotechnologists for several different laboratory positions in the south-west suburbs of Chicago. Position schedule (time and weekdays) are reasonably flexible. Excellent working environments; newer labs and equipment; friendly people. Send CV and availability to SRuby@4path.com Thank you. Dr. Ruby From tony.henwood <@t> health.nsw.gov.au Tue Dec 20 16:10:00 2011 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Dec 20 16:10:17 2011 Subject: [Histonet] Has anyone else noticed In-Reply-To: <5B631BC782D14A94996949A9A8E12AF0@JoePC> References: <6D6BD1DE8A5571489398B392A38A715760A0C11B@xmdb02.nch.kids> <5B631BC782D14A94996949A9A8E12AF0@JoePC> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A0C490@xmdb02.nch.kids> Remember we must validate, repeat, validate, repeate, vaallidatee, reeepeeeeeeaaat, valid......... OMG its blurry again Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Tuesday, 20 December 2011 11:26 AM To: Tony Henwood (SCHN); 'Della Speranza, Vinnie'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Has anyone else noticed I wonder if it works with Scotch. Hmmm, seems like an experiment brewing. JTT ----- Original Message ----- From: "Tony Henwood (SCHN)" To: "'Della Speranza, Vinnie'" ; Sent: Monday, December 19, 2011 4:04 PM Subject: RE: [Histonet] Has anyone else noticed I keep trying to verify this scientific discovery but everything is blurry by the time I get to the bottom of the bottle, Oh sorry I need to look in the glass? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, 20 December 2011 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Has anyone else noticed ________________________________ From: Pam Barker [mailto:relia1@earthlink.net] Sent: Monday, December 19, 2011 10:57 AM To: Della Speranza, Vinnie Subject: RE: [Histonet] Has anyone else noticed hahahahaha Vinnie! A nice Cab is a great anesthetic, and your observation is a great reason to open a bottle!! Who's with me? Happy Holidays!! -----Original Message----- From: "histonet-bounces@lists.utsouthwestern.edu" Sent: 12/19/2011 10:31 AM To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Has anyone else noticed Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Tue Dec 20 16:18:43 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Dec 20 16:18:56 2011 Subject: [Histonet] Has anyone else noticed In-Reply-To: <6D6BD1DE8A5571489398B392A38A715760A0C490@xmdb02.nch.kids> References: <6D6BD1DE8A5571489398B392A38A715760A0C11B@xmdb02.nch.kids> <5B631BC782D14A94996949A9A8E12AF0@JoePC> <6D6BD1DE8A5571489398B392A38A715760A0C490@xmdb02.nch.kids> Message-ID: Joe, you should never have water anywhere near Scotch. There's more than enough water used in its making (uisge beatha - water of life) Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Tuesday, December 20, 2011 5:10 PM To: 'Joe Nocito'; 'Della Speranza, Vinnie'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Has anyone else noticed Remember we must validate, repeat, validate, repeate, vaallidatee, reeepeeeeeeaaat, valid......... OMG its blurry again Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Tuesday, 20 December 2011 11:26 AM To: Tony Henwood (SCHN); 'Della Speranza, Vinnie'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Has anyone else noticed I wonder if it works with Scotch. Hmmm, seems like an experiment brewing. JTT ----- Original Message ----- From: "Tony Henwood (SCHN)" To: "'Della Speranza, Vinnie'" ; Sent: Monday, December 19, 2011 4:04 PM Subject: RE: [Histonet] Has anyone else noticed I keep trying to verify this scientific discovery but everything is blurry by the time I get to the bottom of the bottle, Oh sorry I need to look in the glass? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, 20 December 2011 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Has anyone else noticed ________________________________ From: Pam Barker [mailto:relia1@earthlink.net] Sent: Monday, December 19, 2011 10:57 AM To: Della Speranza, Vinnie Subject: RE: [Histonet] Has anyone else noticed hahahahaha Vinnie! A nice Cab is a great anesthetic, and your observation is a great reason to open a bottle!! Who's with me? Happy Holidays!! -----Original Message----- From: "histonet-bounces@lists.utsouthwestern.edu" Sent: 12/19/2011 10:31 AM To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Has anyone else noticed Wondering if anyone else has noticed that rinsing a wine glass with water will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin staining solution) ?? Yes, with so little positive in the news these days, I am definitely anesthetizing myself this holiday season. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From histology.houston <@t> yahoo.com Tue Dec 20 16:23:23 2011 From: histology.houston <@t> yahoo.com (Maria T) Date: Tue Dec 20 16:23:27 2011 Subject: [Histonet] Tech needed in Houston Message-ID: <1324419803.10898.YahooMailNeo@web140516.mail.bf1.yahoo.com> Histo Tech needed for a small GI lab in Houston with flexible hours. Please email me directly with any questions Thanks From Robyn.Rosenberg <@t> amnhealthcare.com Tue Dec 20 17:11:24 2011 From: Robyn.Rosenberg <@t> amnhealthcare.com (Robyn Rosenberg) Date: Tue Dec 20 17:11:30 2011 Subject: [Histonet] ASCP Histotech-Relo Paid ,TX Message-ID: <2030E2FECB4DAB48B4C31D919380F3FF10A20034@amnmail.ahs.int> **Award Winning Facility in West Texas is Hiring Due to Largest Expansion in History!!!** Winner of the Gallup "Great Place to Work" Award for 5 years in a row!!! 185 Miles West of Dallas; Very Low Cost of Living Relocation assistance - up to $3000 ($1000-1500 within TX) Certified Histotechnologist or Certified Histotechnician Position Summary: Cuts, stains, mounts, and studies specimens of human tissue to provide data on functioning of tissues and organs, causes or progress of disease, following established standards and practices. Experience: HTL (ASCP) Certification Required (Histotechnologist) Bachelor's degree required Experience - 5 years minimum Should be able to embed tissue, cut blocks, staining, process cytology specimens, aid in grossing. Click here to see a benefits summary: www.ehendrick.org/employment/benefits.htm Hours: 8 hour shifts, variable - department is open from 4 am to 5pm Requirements: Histo Cert (ASCP), B.S. in related field, 5+ yrs current histo experience Contact Robyn Rosenberg at 888-609-5271 Robyn Rosenberg Recruiter, Recruitment Process Outsourcing AMN Healthcare Direct Phone: (858) 314-7460 Direct Fax: (866) 652-6931 12400 High Bluff Drive, San Diego CA 92130 robyn.rosenberg@amnhealthcare.com www.amnhealthcare.com NYSE: AHS From one_angel_secret <@t> yahoo.com Tue Dec 20 17:12:13 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Dec 20 17:12:25 2011 Subject: [Histonet] Question about Bile Stain Control tissue In-Reply-To: References: Message-ID: <68B72710-1ADA-49BE-A8C8-5AC43383F31D@yahoo.com> Have you tried gall bladder? And some times livers that are diseased have it. They always look green. Just a thought. Sent from my iPhone On Dec 20, 2011, at 3:45 PM, "Thurby, Christina" wrote: > Hello All, > I have a few questions about Bile Stain control tissue: First - does anyone have or know of any good sources where I can purchase a couple of positive control blocks demonstrating bile in the tissue? I have some purchased control slides - but they appear to be from 'bile sludge' prepared to block. This is a nice emerald green when stained with the Hall's method, but our pathologists would like to see positive bile demonstrated in the tissue elements. Carson's newest book has a very nice image. > > Next question: Carson's procedure for the Hall's bile stain describes the positive elements as a emerald green to drab olive in color. We have a case that appears to be positive but the Hall's stain is kind of a camouflage brownish/drab olive green. Is there a chance we're doing something wrong with this procedure? Our purchased 'bile sludge' control slides are a nice emerald green. We also ran the Stein's method - this was completely negative (even on the purchased controls). Any thoughts and/or feedback is appreciated! > Thanks! > Kristie > > Christina Thurby, MLT(ASCP), HT, QIHC > Research Scientist I > Bristol Myers Squibb > 812-307-2093 > > > > > ________________________________ > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology.houston <@t> yahoo.com Tue Dec 20 19:13:09 2011 From: histology.houston <@t> yahoo.com (Maria T) Date: Tue Dec 20 19:13:13 2011 Subject: [Histonet] Houston Histo Tech Message-ID: <1324429989.96814.YahooMailNeo@web140501.mail.bf1.yahoo.com> ? Histo Tech needed for a small GI lab in Houston with flexible hours. Please email me directly with any questions Thanks Houston.histology@yahoo.com From Stephen.Clark1 <@t> hcahealthcare.com Wed Dec 21 09:25:52 2011 From: Stephen.Clark1 <@t> hcahealthcare.com (Stephen.Clark1@hcahealthcare.com) Date: Wed Dec 21 09:25:57 2011 Subject: [Histonet] control block log Message-ID: <213A638666ECDB4A8A81B5CF8646CC0B745933F6ED@NADCWPMSGCMS05.hca.corpad.net> Hi. I was wondering if anyone would be willing to share a copy of their control block log. I'm creating a new one and want to see what others are doing, for reference. I'm just looking for a blank one. Thanks, Steve Clark Histology Supervisor Grand Strand Regional Medical Center 843-692-1486 Lab 843-692-1459 Desk Stephen.Clark1@hcahealthcare.com [cid:image001.gif@01C9DADA.65BB9E10] From MSHERWOOD <@t> PARTNERS.ORG Wed Dec 21 10:52:54 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Wed Dec 21 10:52:19 2011 Subject: [Histonet] Control Slides Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA2@PHSXMB30.partners.org> To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From trathborne <@t> somerset-healthcare.com Wed Dec 21 11:00:45 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Dec 21 11:01:13 2011 Subject: [Histonet] Control Slides In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA2@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA2@PHSXMB30.partners.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7658B@SMCMAIL01.somerset-healthcare.com> We get ours from Globe http://www.globescientific.com/ Their prices are reasonable, and we have not had any problems with either ihc or special stains. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, December 21, 2011 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Control Slides To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From rjbuesa <@t> yahoo.com Wed Dec 21 11:05:28 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 21 11:05:36 2011 Subject: [Histonet] Control Slides In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA2@PHSXMB30.partners.org> Message-ID: <1324487128.74623.YahooMailClassic@web65715.mail.ac4.yahoo.com> I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. Ren? J.? --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Dec 21 11:09:24 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Dec 21 11:09:34 2011 Subject: [Histonet] Control Slides In-Reply-To: <1324487128.74623.YahooMailClassic@web65715.mail.ac4.yahoo.com> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA2@PHSXMB30.partners.org> <1324487128.74623.YahooMailClassic@web65715.mail.ac4.yahoo.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E19D2BE@evcspmbx3.ads.northwestern.edu> Same here Ren?, unless we can absolutely not find a positive case. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 21, 2011 11:05 AM To: histonet@lists.utsouthwestern.edu; MargaretSherwood Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. Ren? J.? --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Essex <@t> kingstonhospital.nhs.uk Wed Dec 21 11:11:12 2011 From: David.Essex <@t> kingstonhospital.nhs.uk (Essex, David) Date: Wed Dec 21 11:11:33 2011 Subject: [Histonet] Control Slides References: <20111221170637.EFE7544A9A5@nhs-pd1e-esg001.ad1.nhs.net> Message-ID: <20111221171112.83833448634@nhs-pd1e-esg102.ad1.nhs.net> How could you be sure those cases were positive if you didn't have a positive control before, the eternal palindrome....... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 December 2011 17:05 To: histonet@lists.utsouthwestern.edu; MargaretSherwood Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. Ren? J.? --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email is covered by the Kingston Hospital NHS Trust email disclaimer http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer From MSHERWOOD <@t> PARTNERS.ORG Wed Dec 21 11:15:37 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Wed Dec 21 11:15:00 2011 Subject: [Histonet] Control Slides In-Reply-To: <1324487128.74623.YahooMailClassic@web65715.mail.ac4.yahoo.com> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA2@PHSXMB30.partners.org> <1324487128.74623.YahooMailClassic@web65715.mail.ac4.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA3@PHSXMB30.partners.org> That is ideal Rene, but we are a research lab and don't run the same tissue all the time. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, December 21, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. Ren? J. --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Dec 21 11:21:32 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Dec 21 11:21:39 2011 Subject: [Histonet] proximity of proteins for co-localization via IF staining Message-ID: <1324488092.8077.YahooMailNeo@web130101.mail.mud.yahoo.com> Hi Everyone, ? Does anyone know how close in proximity 2 proteins need to be for them to show up as co-localized via immunofluorescence staining? ? I am trying to compare routine 2-color IF vs. the Proximity Ligation Assay (which will not show co-localization unless the proteins are withing 40 nM of each other). ? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From rjbuesa <@t> yahoo.com Wed Dec 21 11:34:49 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 21 11:34:53 2011 Subject: [Histonet] Control Slides In-Reply-To: <20111221171112.83833448634@nhs-pd1e-esg102.ad1.nhs.net> Message-ID: <1324488889.56000.YahooMailClassic@web65709.mail.ac4.yahoo.com> There was always a "first" positive that was used to evaluate the following and so on...so on... etc Besides, you know what to expect with a procedure and when you find it in some tissue, you know it. "Elemental dear Watson". Merry Christmas Ren? J. --- On Wed, 12/21/11, Essex, David wrote: From: Essex, David Subject: RE: [Histonet] Control Slides To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, "MargaretSherwood" Date: Wednesday, December 21, 2011, 12:11 PM How could you be sure those cases were positive if you didn't have a positive control before, the eternal palindrome....... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 21 December 2011 17:05 To: histonet@lists.utsouthwestern.edu; MargaretSherwood Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. Ren? J.? --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email is covered by the Kingston Hospital NHS Trust email disclaimer http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer From rjbuesa <@t> yahoo.com Wed Dec 21 11:36:27 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 21 11:36:31 2011 Subject: [Histonet] Control Slides In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA3@PHSXMB30.partners.org> Message-ID: <1324488987.20130.YahooMailClassic@web65716.mail.ac4.yahoo.com> Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. Ren? J. --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] Control Slides To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 12:15 PM That is ideal Rene, but we are a research lab and don't run the same tissue all the time. ? Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org ? From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, December 21, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. Ren? J.? --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heidi.Hawthorne <@t> onassignment.com Wed Dec 21 11:41:24 2011 From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne) Date: Wed Dec 21 11:41:34 2011 Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! Message-ID: <26C4A3B38503BC4CBBF4D986C3BC9B832290D1F5C6@oasslcexm01.oaifield.onasgn.com> Hello, We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco. Approximately 3 month assignment, day/early morning shift available working in a hospital lab. Requirements: At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. Email your resume today for immediate consideration! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 Heidi.Hawthorne@onassignment.com www.onassignment.com NASDAQ: ASGN People First. Find me on LinkedIn at: http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 From Marilyn.A.Weiss <@t> kp.org Wed Dec 21 12:01:33 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Dec 21 12:03:54 2011 Subject: [Histonet] out of office at noon today Message-ID: I will be out of the office starting 12/20/2011 and will not return until 12/27/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. I will be in town for some of the time. Thank you. From silvinamolinuevo <@t> yahoo.com.ar Wed Dec 21 13:07:15 2011 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Wed Dec 21 13:08:22 2011 Subject: [Histonet] TRAP Staining Message-ID: <1324494435.42985.YahooMailNeo@web113610.mail.gq1.yahoo.com> Dear Dave, we use the next protocol with pretty good results. For diazotation reaction (Solution A): Dissolve 7mg? Fast Garnet GBC base in 1ml of methyl or ethylenglygol. Add 1ml HCl 0.1N. Add drop by drop 2.07mg NO2Na. Keep T below 15?C. Solution B:12.5mg/ml Naftol ASBI phosphate in distilled water For the enzymatic reaction: Disolve 75mg Sodium tartrate in 45 ml Citrate buffer pH 4.9. Pre-warm to 37?C Add 2ml Solution A 0.5ml Solution B Incubate the slides about 1h at 37?C. The most difficult thing is the diazotation reaction. You must have the stoichiometric relationship between Fast Garnet and Sodium nitrite. Sodium nitrite must be as pure as you can. You also have to retain the stoichiometry relation with chlorhidric acid to avoid reaction subproducts. Diazotation T must be not over 15?C and you should use the diazotation product Best regards, silvina Dr Mar?a Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina www.biol.unlp.edu.ar/giomm e-mail: silvina.molinuevo@bigfoot.com From Rcartun <@t> harthosp.org Wed Dec 21 18:28:19 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Dec 21 18:28:27 2011 Subject: [Histonet] control block log In-Reply-To: <213A638666ECDB4A8A81B5CF8646CC0B745933F6ED@NADCWPMSGCMS05.hca.corpad.net> References: <213A638666ECDB4A8A81B5CF8646CC0B745933F6ED@NADCWPMSGCMS05.hca.corpad.net> Message-ID: <4EF23353.7400.0077.0@harthosp.org> I use FileMaker Pro software to keep track of our IHC controls. I created a control tissue file that includes the following: Control #, Case #, Tissue site, Diagnosis, DOS, Time in formalin, Processing date, Number of blocks submitted, and then a "Comment" field for the immunoreactivity for each antibody tested. Every file can then be searched by anyone of the data fields. For example, if we need a control for Parvovirus we would type in "Parvovirus" in the "Diagnosis" or "Comment" fields and then every control tissue tested positive (or negative) for Parvovirus would be identified. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 12/21/2011 10:25 AM >>> Hi. I was wondering if anyone would be willing to share a copy of their control block log. I'm creating a new one and want to see what others are doing, for reference. I'm just looking for a blank one. Thanks, Steve Clark Histology Supervisor Grand Strand Regional Medical Center 843-692-1486 Lab 843-692-1459 Desk Stephen.Clark1@hcahealthcare.com [cid:image001.gif@01C9DADA.65BB9E10] From naveedafahim <@t> yahoo.ca Wed Dec 21 21:19:44 2011 From: naveedafahim <@t> yahoo.ca (naveeda arshad) Date: Wed Dec 21 21:19:48 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 Message-ID: <1324523984.99929.YahooMailClassic@web161704.mail.bf1.yahoo.com> Hi?Does any one has idea about how to make in house gram ?Positive and negative control in your lab. What kind of tissue is suitable and and i need detail?procedure?for that.Thanks --- On Wed, 12/21/11, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 97, Issue 25 To: histonet@lists.utsouthwestern.edu Received: Wednesday, December 21, 2011, 10:17 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. RE: Control Slides (Rene J Buesa) ???2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) ---------------------------------------------------------------------- Message: 1 Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) From: Rene J Buesa Subject: RE: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu,??? MargaretSherwood ??? Message-ID: ??? <1324488987.20130.YahooMailClassic@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. Ren? J. --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] Control Slides To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 12:15 PM That is ideal Rene, but we are a research lab and don't run the same tissue all the time. ? Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org ? From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, December 21, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. Ren? J.? --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 21 Dec 2011 09:41:24 -0800 From: Heidi Hawthorne Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <26C4A3B38503BC4CBBF4D986C3BC9B832290D1F5C6@oasslcexm01.oaifield.onasgn.com> ??? Content-Type: text/plain; charset="us-ascii" Hello, We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco.? Approximately 3 month assignment, day/early morning shift available working in a hospital lab.? Requirements:? At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. Email your resume today for immediate consideration! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 Heidi.Hawthorne@onassignment.com www.onassignment.com NASDAQ: ASGN People First. Find me on LinkedIn at:? http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 97, Issue 25 **************************************** From sauconym <@t> yahoo.com Thu Dec 22 01:07:26 2011 From: sauconym <@t> yahoo.com (Miha Tesar) Date: Thu Dec 22 01:08:31 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 In-Reply-To: <1324523984.99929.YahooMailClassic@web161704.mail.bf1.yahoo.com> References: <1324523984.99929.YahooMailClassic@web161704.mail.bf1.yahoo.com> Message-ID: <1324537646.75201.YahooMailNeo@web45015.mail.sp1.yahoo.com> Haloha! Does any one has any?idea about how to cut the fat tissue in cryostat (frozen section). Temperature of the chambre/specimen and the thickness of the slice. I use the cryo spray but does not help me much! THX Miha and Mary C. 2 all ________________________________ From: naveeda arshad To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 22, 2011 4:19 AM Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 Hi?Does? make in house gram ?Positive and negative control in your lab. What kind of tissue is suitable and and i need detail?procedure?for that.Thanks --- On Wed, 12/21/11, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 97, Issue 25 To: histonet@lists.utsouthwestern.edu Received: Wednesday, December 21, 2011, 10:17 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. RE: Control Slides (Rene J Buesa) ???2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) ---------------------------------------------------------------------- Message: 1 Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) From: Rene J Buesa Subject: RE: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu,??? MargaretSherwood ??? Message-ID: ??? <1324488987.20130.YahooMailClassic@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. Ren? J. --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] Control Slides To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 12:15 PM That is ideal Rene, but we are a research lab and don't run the same tissue all the time. ? Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org ? From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, December 21, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. Ren? J.? --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 21 Dec 2011 09:41:24 -0800 From: Heidi Hawthorne Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <26C4A3B38503BC4CBBF4D986C3BC9B832290D1F5C6@oasslcexm01.oaifield.onasgn.com> ??? Content-Type: text/plain; charset="us-ascii" Hello, We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco.? Approximately 3 month assignment, day/early morning shift available working in a hospital lab.? Requirements:? At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. Email your resume today for immediate consideration! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 Heidi.Hawthorne@onassignment.com www.onassignment.com NASDAQ: ASGN People First. Find me on LinkedIn at:? http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 97, Issue 25 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sruby <@t> 4path.com Thu Dec 22 08:16:05 2011 From: Sruby <@t> 4path.com (Stephen G. Ruby) Date: Thu Dec 22 08:16:10 2011 Subject: [Histonet] Position Update - SW Chicago Suburbs Message-ID: I had previously provided information on part time positions in the SW suburban location in Chicago. Currently, one of those positions has been upgraded to a full time position. If interested, please provide a CV and letter to me, and I will then forward. Thank you! Have a wonderful Holiday. Dr. Ruby From madary <@t> verizon.net Thu Dec 22 09:44:20 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Thu Dec 22 09:44:33 2011 Subject: [Histonet] equipment and supply donations for start up non profit histolab in DC Message-ID: <21850240.843147.1324568660991.JavaMail.root@vms170035> I am usually the one giving stuff away and I now find myself in need of a handout. If anyone in histoland has any items they can get rid of please email me back so we can work out some details. I can use anything in terms of supplies, expendables, equipment. I have a microtome and water bath past that I have an empty room. Many thanks! Nick(Rocky) Madary, HT/HTL(ASCP)QIHC From silvinamolinuevo <@t> yahoo.com.ar Thu Dec 22 12:25:35 2011 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Thu Dec 22 12:25:38 2011 Subject: [Histonet] Hard tissue microtome Message-ID: <1324578335.38872.YahooMailNeo@web113606.mail.gq1.yahoo.com> Hi! could??anyone recommend me ?a good microtome for hard tissue (specifically for bone tissue)? Thanks' in advance Hope you all have a good nativity and happy new year! silvina From madeleinehuey <@t> gmail.com Thu Dec 22 12:54:17 2011 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Dec 22 12:54:23 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 26 In-Reply-To: <4ef3705a.042c650a.313a.ffffeb09SMTPIN_ADDED@mx.google.com> References: <4ef3705a.042c650a.313a.ffffeb09SMTPIN_ADDED@mx.google.com> Message-ID: Miha, You need to lower down the cryostat at least -25 degree. You also need to spray your block the very minute before the cutting (not just one time spray on the OCT block). The thicker the section, the easier it can be cut. I also put my OCT blocks on the dry ice next to me, and transfer to the cryostat when I am ready to cut. Good Luck (I know it's not easy)! Madeleine Supervisor-Pathology (IPOX & Histology) El Camino Hospital On Thu, Dec 22, 2011 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. out of office at noon today (Marilyn.A.Weiss@kp.org) > ? 2. TRAP Staining (Silvina Molinuevo) > ? 3. Re: control block log (Richard Cartun) > ? 4. Re: Histonet Digest, Vol 97, Issue 25 (naveeda arshad) > ? 5. Re: Re: Histonet Digest, Vol 97, Issue 25 (Miha Tesar) > ? 6. Position Update - SW Chicago Suburbs (Stephen G. Ruby) > ? 7. equipment and supply donations for start up non profit > ? ? ?histolab in DC (madary@verizon.net) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 21 Dec 2011 10:01:33 -0800 > From: Marilyn.A.Weiss@kp.org > Subject: [Histonet] out of office at noon today > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=US-ASCII > > > > I will be out of the office starting ?12/20/2011 and will not return until > 12/27/2011. > > In my absence please ask for Mary . ?If this is urgent or you need to speak > to me directly ?you can contact me on my cell phone number 858-472-4266. If > it concerns a Mohs to be scheduled you can e-mail me or call on my cell. ?I > will be in town for some of the time. > Thank you. > > ------------------------------ > > Message: 2 > Date: Wed, 21 Dec 2011 11:07:15 -0800 (PST) > From: Silvina Molinuevo > Subject: [Histonet] TRAP Staining > To: "darko@uab.edu" > Cc: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1324494435.42985.YahooMailNeo@web113610.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dear Dave, we use the next protocol with pretty good results. > > > For diazotation reaction (Solution A): > Dissolve 7mg? Fast Garnet GBC base in 1ml of methyl or ethylenglygol. Add 1ml HCl 0.1N. Add drop by drop 2.07mg NO2Na. Keep T below 15?C. > > Solution B:12.5mg/ml Naftol ASBI phosphate in distilled water > > For the enzymatic reaction: > > Disolve 75mg Sodium tartrate in 45 ml Citrate buffer pH 4.9. Pre-warm to 37?C > > Add > > 2ml Solution A > 0.5ml Solution B > > Incubate the slides about 1h at 37?C. > > > The most difficult thing is the diazotation reaction. > > You must have the stoichiometric relationship between Fast Garnet and Sodium nitrite. Sodium nitrite must be as pure as you can. You also have to retain the stoichiometry relation with chlorhidric acid to avoid reaction subproducts. Diazotation T must be not over 15?C and you should use the diazotation product > > > Best regards, silvina > > > Dr Mar?a Silvina Molinuevo > Grupo de Investigacion en > Osteopatias y Metabolismo Mineral > Departamento de Ciencias Biologicas > Facultad de Ciencias Exactas > Universidad Nacional de La Plata > 47 y 115 > (1900)La Plata > Argentina > www.biol.unlp.edu.ar/giomm > e-mail: silvina.molinuevo@bigfoot.com > > ------------------------------ > > Message: 3 > Date: Wed, 21 Dec 2011 19:28:19 -0500 > From: "Richard Cartun" > Subject: Re: [Histonet] control block log > To: , > ? ? ? ? > Message-ID: <4EF23353.7400.0077.0@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > I use FileMaker Pro software to keep track of our IHC controls. ?I created a control tissue file that includes the following: ?Control #, Case #, Tissue site, Diagnosis, DOS, Time in formalin, Processing date, Number of blocks submitted, and then a "Comment" field for the immunoreactivity for each antibody tested. ?Every file can then be searched by anyone of the data fields. ?For example, if we need a control for Parvovirus we would type in "Parvovirus" in the "Diagnosis" or "Comment" fields and then every control tissue tested positive (or negative) for Parvovirus would be identified. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT ?06102 > (860) 545-1596?Office > (860) 545-2204?Fax > > >>>> 12/21/2011 10:25 AM >>> > > Hi. ?I was wondering if anyone would be willing to share a copy of their control block log. ?I'm creating a new one and want to see what others are doing, for reference. ?I'm just looking for a blank one. > Thanks, > > Steve Clark > Histology Supervisor > Grand Strand Regional Medical Center > 843-692-1486?Lab > 843-692-1459?Desk > Stephen.Clark1@hcahealthcare.com > > ?[cid:image001.gif@01C9DADA.65BB9E10] > > > > > > > > ------------------------------ > > Message: 4 > Date: Wed, 21 Dec 2011 19:19:44 -0800 (PST) > From: naveeda arshad > Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ?<1324523984.99929.YahooMailClassic@web161704.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi?Does any one has idea about how to make in house gram ?Positive and negative control in your lab. What kind of tissue is suitable and and i need detail?procedure?for that.Thanks > > --- On Wed, 12/21/11, histonet-request@lists.utsouthwestern.edu wrote: > > From: histonet-request@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 97, Issue 25 > To: histonet@lists.utsouthwestern.edu > Received: Wednesday, December 21, 2011, 10:17 PM > > Send Histonet mailing list submissions to > ??? histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ??? histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ??? histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ???1. RE: Control Slides (Rene J Buesa) > ???2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) > From: Rene J Buesa > Subject: RE: [Histonet] Control Slides > To: histonet@lists.utsouthwestern.edu,??? MargaretSherwood > ??? > Message-ID: > ??? <1324488987.20130.YahooMailClassic@web65716.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. > Ren? J. > > --- On Wed, 12/21/11, Sherwood, Margaret wrote: > > > From: Sherwood, Margaret > Subject: RE: [Histonet] Control Slides > To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu > Date: Wednesday, December 21, 2011, 12:15 PM > > > > > That is ideal Rene, but we are a research lab and don't run the same tissue all the time. > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839?(voice mail) > 617-726-6983?(lab) > 617-726-1206?(fax) > msherwood@partners.org > > > > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Wednesday, December 21, 2011 12:05 PM > To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret > Subject: Re: [Histonet] Control Slides > > > > > > > > I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. > Ren? J. > > --- On Wed, 12/21/11, Sherwood, Margaret wrote: > > > From: Sherwood, Margaret > Subject: Re: [Histonet] Control Slides > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, December 21, 2011, 11:52 AM > > > To all: > > I know this has come up before, but where do most people buy their (+) control > slides for special stains? > > Thanks! > Peggy > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839?(voice mail) > 617-726-6983?(lab) > 617-726-1206?(fax) > msherwood@partners.org > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Wed, 21 Dec 2011 09:41:24 -0800 > From: Heidi Hawthorne > Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! > To: "histonet@lists.utsouthwestern.edu" > ??? > Message-ID: > ??? <26C4A3B38503BC4CBBF4D986C3BC9B832290D1F5C6@oasslcexm01.oaifield.onasgn.com> > > Content-Type: text/plain; charset="us-ascii" > > Hello, > > We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco.? Approximately 3 month assignment, day/early morning shift available working in a hospital lab.? Requirements:? At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. > > Email your resume today for immediate consideration! > > Heidi Hawthorne > Sr. Account Executive > On Assignment, Inc. > t: (510) 663-8622 > c: (510) 435-7326 > f: (866) 741-0805 > Heidi.Hawthorne@onassignment.com > www.onassignment.com > NASDAQ: ASGN > > > People First. > > Find me on LinkedIn at:? http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 25 > **************************************** > > > ------------------------------ > > Message: 5 > Date: Wed, 21 Dec 2011 23:07:26 -0800 (PST) > From: Miha Tesar > Subject: Re: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 > To: naveeda arshad , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1324537646.75201.YahooMailNeo@web45015.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Haloha! > Does any one has any?idea about how to cut the fat tissue in cryostat (frozen section). Temperature of the chambre/specimen and the thickness of the slice. I use the cryo spray but does not help me much! > THX Miha and Mary C. 2 all > > > > ________________________________ > From: naveeda arshad > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, December 22, 2011 4:19 AM > Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 > > Hi?Does? make in house gram ?Positive and negative control in your lab. What kind of tissue is suitable and and i need detail?procedure?for that.Thanks > > --- On Wed, 12/21/11, histonet-request@lists.utsouthwestern.edu wrote: > > From: histonet-request@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 97, Issue 25 > To: histonet@lists.utsouthwestern.edu > Received: Wednesday, December 21, 2011, 10:17 PM > > Send Histonet mailing list submissions to > ??? histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ??? histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ??? histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ???1. RE: Control Slides (Rene J Buesa) > ???2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) > From: Rene J Buesa > Subject: RE: [Histonet] Control Slides > To: histonet@lists.utsouthwestern.edu,??? MargaretSherwood > ??? > Message-ID: > ??? <1324488987.20130.YahooMailClassic@web65716.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. > Ren? J. > > --- On Wed, 12/21/11, Sherwood, Margaret wrote: > > > From: Sherwood, Margaret > Subject: RE: [Histonet] Control Slides > To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu > Date: Wednesday, December 21, 2011, 12:15 PM > > > > > That is ideal Rene, but we are a research lab and don't run the same tissue all the time. > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839?(voice mail) > 617-726-6983?(lab) > 617-726-1206?(fax) > msherwood@partners.org > > > > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Wednesday, December 21, 2011 12:05 PM > To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret > Subject: Re: [Histonet] Control Slides > > > > > > > > I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. > Ren? J. > > --- On Wed, 12/21/11, Sherwood, Margaret wrote: > > > From: Sherwood, Margaret > Subject: Re: [Histonet] Control Slides > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, December 21, 2011, 11:52 AM > > > To all: > > I know this has come up before, but where do most people buy their (+) control > slides for special stains? > > Thanks! > Peggy > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839?(voice mail) > 617-726-6983?(lab) > 617-726-1206?(fax) > msherwood@partners.org > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Wed, 21 Dec 2011 09:41:24 -0800 > From: Heidi Hawthorne > Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! > To: "histonet@lists.utsouthwestern.edu" > ??? > Message-ID: > ??? <26C4A3B38503BC4CBBF4D986C3BC9B832290D1F5C6@oasslcexm01.oaifield.onasgn.com> > > Content-Type: text/plain; charset="us-ascii" > > Hello, > > We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco.? Approximately 3 month assignment, day/early morning shift available working in a hospital lab.? Requirements:? At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. > > Email your resume today for immediate consideration! > > Heidi Hawthorne > Sr. Account Executive > On Assignment, Inc. > t: (510) 663-8622 > c: (510) 435-7326 > f: (866) 741-0805 > Heidi.Hawthorne@onassignment.com > www.onassignment.com > NASDAQ: ASGN > > > People First. > > Find me on LinkedIn at:? http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 25 > **************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 6 > Date: Thu, 22 Dec 2011 08:16:05 -0600 > From: "Stephen G. Ruby" > Subject: [Histonet] Position Update - SW Chicago Suburbs > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > I had previously provided information on part time positions in the SW suburban location in Chicago. > Currently, one of those positions has been upgraded to a full time position. > > If interested, please provide a CV and letter to me, and I will then forward. > > Thank you! > > Have a wonderful Holiday. > > Dr. Ruby > > > ------------------------------ > > Message: 7 > Date: Thu, 22 Dec 2011 09:44:20 -0600 (CST) > From: madary@verizon.net > Subject: [Histonet] equipment and supply donations for start up non > ? ? ? ?profit ?histolab in DC > To: histonet@lists.utsouthwestern.edu > Message-ID: <21850240.843147.1324568660991.JavaMail.root@vms170035> > Content-Type: text/plain; charset="UTF-8" > > > ? ?I ?am usually the one giving stuff away and I now find myself in need > ? of a handout. If anyone in histoland has any items they can get rid of > ? please ?email ?me ?back ?so ?we ?can ?work out some details. I can use > ? anything ?in ?terms ?of ?supplies, ?expendables, ?equipment. ?I have a > ? microtome and water bath past that I have an empty room. Many thanks! > > ? Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 26 > **************************************** From ratliffjack <@t> hotmail.com Thu Dec 22 13:05:09 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Dec 22 13:05:13 2011 Subject: [Histonet] Hard tissue microtome In-Reply-To: <1324578335.38872.YahooMailNeo@web113606.mail.gq1.yahoo.com> References: <1324578335.38872.YahooMailNeo@web113606.mail.gq1.yahoo.com> Message-ID: Can you tell us more about the bone specimens you are wanting to cut? Jack Jack Ratliff Senior Histologist, BioMimetic Therapeutics, Inc. Chairman, Hard Tissue Committee - National Society for Histotechnology > Date: Thu, 22 Dec 2011 10:25:35 -0800 > From: silvinamolinuevo@yahoo.com.ar > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hard tissue microtome > > Hi! could anyone recommend me a good microtome for hard tissue (specifically for bone tissue)? > Thanks' in advance > Hope you all have a good nativity and happy new year! > silvina > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Dec 22 13:10:43 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Dec 22 13:10:47 2011 Subject: [Histonet] Tissue Gram stain controls Message-ID: Naveeda Arshad (where?) asks: >>Does any one has idea about how to make in house gram positive and negative control in your lab? What kind of tissue is suitable and and i need detail procedure for that?<< Usually a section of a ruptured appendix (easy enough to get in a hospital histology lab) will provide an abundance of suitable bacteria. A better solution is not to do a tissue Gram stain at all. You want to see bacteria - you really can't identify them in tissue sections. A simple tissue Giemsa or Diff-Quik II stain is both sensitive and specific for seeing bacteria of all kinds. (The ruptured appendix will work well as a control.) Pathologists are much too ready to order a stain that's of very dubious clinical value, particularly since tissue Gram stains usually stain Gram negative organisms rather weakly. Bob Richmond Samurai Pathologist Knoxville TN From mdpraet <@t> gmail.com Thu Dec 22 13:21:46 2011 From: mdpraet <@t> gmail.com (mequita praet) Date: Thu Dec 22 13:21:50 2011 Subject: [Histonet] Re:sectioning fat Message-ID: Instead of using the cryo sray, use liquid nitrogen. Make a thick cotton swab, soak it in the liquid nitrogen, place it on the fat letting it turn white then discard the first section off then pick up the next one. This works really well. The cryo chamber needs to be really cold as well -25 works best. Let me know if you need more help. I have multiple tricks for cryosectioning. Mequita Praet, HTL(ASCP)SLS From abright <@t> brightinstruments.com Thu Dec 22 14:00:35 2011 From: abright <@t> brightinstruments.com (abright@brightinstruments.com) Date: Thu Dec 22 14:00:35 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 In-Reply-To: <1324537646.75201.YahooMailNeo@web45015.mail.sp1.yahoo.com> References: <1324523984.99929.YahooMailClassic@web161704.mail.bf1.yahoo.com> <1324537646.75201.YahooMailNeo@web45015.mail.sp1.yahoo.com> Message-ID: <1160924152-1324584027-cardhu_decombobulator_blackberry.rim.net-505473258-@b26.c2.bise7.blackberry> I would not use any freezing aerosols if there is any suspicion of infection in tissue or cryochamber has been used for prior sectioning without being decontaminated. Depending on how fatty the tissue is you need to be in the region -28 to -35 degs C on the specimen, knife and anti-roll plate, for that you need a decent cryostat. Best regards Alan Bright Sent from my BlackBerry? wireless device -----Original Message----- From: Miha Tesar Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 21 Dec 2011 23:07:26 To: naveeda arshad; histonet@lists.utsouthwestern.edu Reply-To: Miha Tesar Subject: Re: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 Haloha! Does any one has any?idea about how to cut the fat tissue in cryostat (frozen section). Temperature of the chambre/specimen and the thickness of the slice. I use the cryo spray but does not help me much! THX Miha and Mary C. 2 all ________________________________ From: naveeda arshad To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 22, 2011 4:19 AM Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 Hi?Does? make in house gram ?Positive and negative control in your lab. What kind of tissue is suitable and and i need detail?procedure?for that.Thanks --- On Wed, 12/21/11, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 97, Issue 25 To: histonet@lists.utsouthwestern.edu Received: Wednesday, December 21, 2011, 10:17 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. RE: Control Slides (Rene J Buesa) ???2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) ---------------------------------------------------------------------- Message: 1 Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) From: Rene J Buesa Subject: RE: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu,??? MargaretSherwood ??? Message-ID: ??? <1324488987.20130.YahooMailClassic@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. Ren? J. --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] Control Slides To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 12:15 PM That is ideal Rene, but we are a research lab and don't run the same tissue all the time. ? Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org ? From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, December 21, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. Ren? J.? --- On Wed, 12/21/11, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 21 Dec 2011 09:41:24 -0800 From: Heidi Hawthorne Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <26C4A3B38503BC4CBBF4D986C3BC9B832290D1F5C6@oasslcexm01.oaifield.onasgn.com> ??? Content-Type: text/plain; charset="us-ascii" Hello, We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco.? Approximately 3 month assignment, day/early morning shift available working in a hospital lab.? Requirements:? At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. Email your resume today for immediate consideration! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 Heidi.Hawthorne@onassignment.com www.onassignment.com NASDAQ: ASGN People First. Find me on LinkedIn at:? http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 97, Issue 25 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01Gc7bWvy) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01Gc7bWvy&m=c326c3be8e02&t=20111222&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01Gc7bWvy&m=c326c3be8e02&t=20111222&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01Gc7bWvy&m=c326c3be8e02&t=20111222&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From techonebs <@t> comcast.net Thu Dec 22 16:32:31 2011 From: techonebs <@t> comcast.net (Matt Mincer) Date: Thu Dec 22 16:32:35 2011 Subject: [Histonet] RE. equipment and supply donations Message-ID: <4EF3AFFF.9020607@comcast.net> Hey Rocky, We may have some stuff. Give me a call. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 From one_angel_secret <@t> yahoo.com Thu Dec 22 17:03:42 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Dec 22 17:03:54 2011 Subject: [Histonet] Control Slides In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA2@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5CA2@PHSXMB30.partners.org> Message-ID: <4F7C3FD6-FDBF-4C2E-BC8C-8647F63D1C80@yahoo.com> I've used http://www.americanmastertech.com/store/main.aspx?p=Categorybody&c=CV Only when I couldn't use what we had in the lab. Also some people might trade you if you have something they can use? Another thing I've noticed is occasionally vendors would request a control block for another client. I've always helped when I could so maybe you can ask for help from your vendors? Best of luck. Kim Sent from my iPhone On Dec 21, 2011, at 11:52 AM, "Sherwood, Margaret" wrote: > To all: > > I know this has come up before, but where do most people buy their (+) control > slides for special stains? > > Thanks! > Peggy > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839 (voice mail) > 617-726-6983 (lab) > 617-726-1206 (fax) > msherwood@partners.org > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Thu Dec 22 17:17:09 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Dec 22 17:17:20 2011 Subject: [Histonet] Tissue Gram stain controls In-Reply-To: References: Message-ID: <854BAB8B-C768-4994-99C1-F46138EF9259@yahoo.com> But if you don't have a pathologist as compassionate as Dr Richmond and you are bound to do it. I would get microbiology to grow you a batch. Then plate/insert them into a section of lung. I prefer lung because it provides some background but not too much. This is just one way I've seen this done a long time ago. Good luck Kim Donadio Sent from my iPhone On Dec 22, 2011, at 2:10 PM, Bob Richmond wrote: > Naveeda Arshad (where?) asks: >>Does any one has idea about how to > make in house gram positive and negative control in your lab? What > kind of tissue is suitable and and i need detail procedure for that?<< > > Usually a section of a ruptured appendix (easy enough to get in a > hospital histology lab) will provide an abundance of suitable > bacteria. > > A better solution is not to do a tissue Gram stain at all. You want to > see bacteria - you really can't identify them in tissue sections. A > simple tissue Giemsa or Diff-Quik II stain is both sensitive and > specific for seeing bacteria of all kinds. (The ruptured appendix will > work well as a control.) Pathologists are much too ready to order a > stain that's of very dubious clinical value, particularly since tissue > Gram stains usually stain Gram negative organisms rather weakly. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Thu Dec 22 22:16:54 2011 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Dec 22 22:17:01 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 26 In-Reply-To: References: <4ef3705a.042c650a.313a.ffffeb09SMTPIN_ADDED@mx.google.com> Message-ID: Naveeda, You can use a piece of fresh placenta or lung and just add the (+) & (-) gram bacteria (microbiology lab always have them in culture) to the tissues. Let the bugs infect the tissues for overnight @ room temperature or in a bacteria incubator (if you have one) @ 37c for overnight. I just add enough saline to the tissue and keep it moist. I chose placenta because it is easy to obtain (if there is a maternal ward, then plenty placenta after the delivery). Good Luck! Madeleine Supervisor-Pathology (IPOX & Histology) El Camino Hospital 2500 Grant Road Mountain View, CA 94040 madeleine_h@elcaminohospital.org On Thu, Dec 22, 2011 at 10:54 AM, Madeleine Huey wrote: > Miha, > You need to lower down the cryostat at least -25 degree. You also need > to spray your block the very minute before the cutting (not just one > time spray on the OCT block). ?The thicker the section, the easier it > can be cut. > I also put my OCT blocks on the dry ice next to me, and transfer to > the cryostat when I am ready to cut. > Good Luck (I know it's not easy)! > Madeleine > Supervisor-Pathology (IPOX & Histology) > El Camino Hospital > > On Thu, Dec 22, 2011 at 10:00 AM, > wrote: >> Send Histonet mailing list submissions to >> ? ? ? ?histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> ? ? ? ?histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> ? ? ? ?histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> ? 1. out of office at noon today (Marilyn.A.Weiss@kp.org) >> ? 2. TRAP Staining (Silvina Molinuevo) >> ? 3. Re: control block log (Richard Cartun) >> ? 4. Re: Histonet Digest, Vol 97, Issue 25 (naveeda arshad) >> ? 5. Re: Re: Histonet Digest, Vol 97, Issue 25 (Miha Tesar) >> ? 6. Position Update - SW Chicago Suburbs (Stephen G. Ruby) >> ? 7. equipment and supply donations for start up non profit >> ? ? ?histolab in DC (madary@verizon.net) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Wed, 21 Dec 2011 10:01:33 -0800 >> From: Marilyn.A.Weiss@kp.org >> Subject: [Histonet] out of office at noon today >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset=US-ASCII >> >> >> >> I will be out of the office starting ?12/20/2011 and will not return until >> 12/27/2011. >> >> In my absence please ask for Mary . ?If this is urgent or you need to speak >> to me directly ?you can contact me on my cell phone number 858-472-4266. If >> it concerns a Mohs to be scheduled you can e-mail me or call on my cell. ?I >> will be in town for some of the time. >> Thank you. >> >> ------------------------------ >> >> Message: 2 >> Date: Wed, 21 Dec 2011 11:07:15 -0800 (PST) >> From: Silvina Molinuevo >> Subject: [Histonet] TRAP Staining >> To: "darko@uab.edu" >> Cc: "histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ?<1324494435.42985.YahooMailNeo@web113610.mail.gq1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Dear Dave, we use the next protocol with pretty good results. >> >> >> For diazotation reaction (Solution A): >> Dissolve 7mg? Fast Garnet GBC base in 1ml of methyl or ethylenglygol. Add 1ml HCl 0.1N. Add drop by drop 2.07mg NO2Na. Keep T below 15?C. >> >> Solution B:12.5mg/ml Naftol ASBI phosphate in distilled water >> >> For the enzymatic reaction: >> >> Disolve 75mg Sodium tartrate in 45 ml Citrate buffer pH 4.9. Pre-warm to 37?C >> >> Add >> >> 2ml Solution A >> 0.5ml Solution B >> >> Incubate the slides about 1h at 37?C. >> >> >> The most difficult thing is the diazotation reaction. >> >> You must have the stoichiometric relationship between Fast Garnet and Sodium nitrite. Sodium nitrite must be as pure as you can. You also have to retain the stoichiometry relation with chlorhidric acid to avoid reaction subproducts. Diazotation T must be not over 15?C and you should use the diazotation product >> >> >> Best regards, silvina >> >> >> Dr Mar?a Silvina Molinuevo >> Grupo de Investigacion en >> Osteopatias y Metabolismo Mineral >> Departamento de Ciencias Biologicas >> Facultad de Ciencias Exactas >> Universidad Nacional de La Plata >> 47 y 115 >> (1900)La Plata >> Argentina >> www.biol.unlp.edu.ar/giomm >> e-mail: silvina.molinuevo@bigfoot.com >> >> ------------------------------ >> >> Message: 3 >> Date: Wed, 21 Dec 2011 19:28:19 -0500 >> From: "Richard Cartun" >> Subject: Re: [Histonet] control block log >> To: , >> ? ? ? ? >> Message-ID: <4EF23353.7400.0077.0@harthosp.org> >> Content-Type: text/plain; charset=US-ASCII >> >> I use FileMaker Pro software to keep track of our IHC controls. ?I created a control tissue file that includes the following: ?Control #, Case #, Tissue site, Diagnosis, DOS, Time in formalin, Processing date, Number of blocks submitted, and then a "Comment" field for the immunoreactivity for each antibody tested. ?Every file can then be searched by anyone of the data fields. ?For example, if we need a control for Parvovirus we would type in "Parvovirus" in the "Diagnosis" or "Comment" fields and then every control tissue tested positive (or negative) for Parvovirus would be identified. >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & Immunopathology >> Director, Biospecimen Collection Programs >> Assistant Director, Anatomic Pathology >> Hartford Hospital >> 80 Seymour Street >> Hartford, CT ?06102 >> (860) 545-1596?Office >> (860) 545-2204?Fax >> >> >>>>> 12/21/2011 10:25 AM >>> >> >> Hi. ?I was wondering if anyone would be willing to share a copy of their control block log. ?I'm creating a new one and want to see what others are doing, for reference. ?I'm just looking for a blank one. >> Thanks, >> >> Steve Clark >> Histology Supervisor >> Grand Strand Regional Medical Center >> 843-692-1486?Lab >> 843-692-1459?Desk >> Stephen.Clark1@hcahealthcare.com >> >> ?[cid:image001.gif@01C9DADA.65BB9E10] >> >> >> >> >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Wed, 21 Dec 2011 19:19:44 -0800 (PST) >> From: naveeda arshad >> Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> ? ? ? ?<1324523984.99929.YahooMailClassic@web161704.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Hi?Does any one has idea about how to make in house gram ?Positive and negative control in your lab. What kind of tissue is suitable and and i need detail?procedure?for that.Thanks >> >> --- On Wed, 12/21/11, histonet-request@lists.utsouthwestern.edu wrote: >> >> From: histonet-request@lists.utsouthwestern.edu >> Subject: Histonet Digest, Vol 97, Issue 25 >> To: histonet@lists.utsouthwestern.edu >> Received: Wednesday, December 21, 2011, 10:17 PM >> >> Send Histonet mailing list submissions to >> ??? histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> ??? histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> ??? histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> ???1. RE: Control Slides (Rene J Buesa) >> ???2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) >> From: Rene J Buesa >> Subject: RE: [Histonet] Control Slides >> To: histonet@lists.utsouthwestern.edu,??? MargaretSherwood >> ??? >> Message-ID: >> ??? <1324488987.20130.YahooMailClassic@web65716.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. >> Ren? J. >> >> --- On Wed, 12/21/11, Sherwood, Margaret wrote: >> >> >> From: Sherwood, Margaret >> Subject: RE: [Histonet] Control Slides >> To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu >> Date: Wednesday, December 21, 2011, 12:15 PM >> >> >> >> >> That is ideal Rene, but we are a research lab and don't run the same tissue all the time. >> >> Peggy Sherwood >> Lab Associate, Photopathology >> Wellman Center for Photomedicine (EDR 214) >> Massachusetts General Hospital >> 50 Blossom Street >> Boston, MA 02114-2696 >> 617-724-4839?(voice mail) >> 617-726-6983?(lab) >> 617-726-1206?(fax) >> msherwood@partners.org >> >> >> >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Wednesday, December 21, 2011 12:05 PM >> To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret >> Subject: Re: [Histonet] Control Slides >> >> >> >> >> >> >> >> I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. >> Ren? J. >> >> --- On Wed, 12/21/11, Sherwood, Margaret wrote: >> >> >> From: Sherwood, Margaret >> Subject: Re: [Histonet] Control Slides >> To: histonet@lists.utsouthwestern.edu >> Date: Wednesday, December 21, 2011, 11:52 AM >> >> >> To all: >> >> I know this has come up before, but where do most people buy their (+) control >> slides for special stains? >> >> Thanks! >> Peggy >> >> Peggy Sherwood >> Lab Associate, Photopathology >> Wellman Center for Photomedicine (EDR 214) >> Massachusetts General Hospital >> 50 Blossom Street >> Boston, MA 02114-2696 >> 617-724-4839?(voice mail) >> 617-726-6983?(lab) >> 617-726-1206?(fax) >> msherwood@partners.org >> >> >> >> The information in this e-mail is intended only for the person to whom it is >> addressed. If you believe this e-mail was sent to you in error and the e-mail >> contains patient information, please contact the Partners Compliance HelpLine at >> http://www.partners.org/complianceline . If the e-mail was sent to you in error >> but does not contain patient information, please contact the sender and properly >> dispose of the e-mail. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 2 >> Date: Wed, 21 Dec 2011 09:41:24 -0800 >> From: Heidi Hawthorne >> Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! >> To: "histonet@lists.utsouthwestern.edu" >> ??? >> Message-ID: >> ??? <26C4A3B38503BC4CBBF4D986C3BC9B832290D1F5C6@oasslcexm01.oaifield.onasgn.com> >> >> Content-Type: text/plain; charset="us-ascii" >> >> Hello, >> >> We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco.? Approximately 3 month assignment, day/early morning shift available working in a hospital lab.? Requirements:? At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. >> >> Email your resume today for immediate consideration! >> >> Heidi Hawthorne >> Sr. Account Executive >> On Assignment, Inc. >> t: (510) 663-8622 >> c: (510) 435-7326 >> f: (866) 741-0805 >> Heidi.Hawthorne@onassignment.com >> www.onassignment.com >> NASDAQ: ASGN >> >> >> People First. >> >> Find me on LinkedIn at:? http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 97, Issue 25 >> **************************************** >> >> >> ------------------------------ >> >> Message: 5 >> Date: Wed, 21 Dec 2011 23:07:26 -0800 (PST) >> From: Miha Tesar >> Subject: Re: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 >> To: naveeda arshad , >> ? ? ? ?"histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ?<1324537646.75201.YahooMailNeo@web45015.mail.sp1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Haloha! >> Does any one has any?idea about how to cut the fat tissue in cryostat (frozen section). Temperature of the chambre/specimen and the thickness of the slice. I use the cryo spray but does not help me much! >> THX Miha and Mary C. 2 all >> >> >> >> ________________________________ >> From: naveeda arshad >> To: histonet@lists.utsouthwestern.edu >> Sent: Thursday, December 22, 2011 4:19 AM >> Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 >> >> Hi?Does? make in house gram ?Positive and negative control in your lab. What kind of tissue is suitable and and i need detail?procedure?for that.Thanks >> >> --- On Wed, 12/21/11, histonet-request@lists.utsouthwestern.edu wrote: >> >> From: histonet-request@lists.utsouthwestern.edu >> Subject: Histonet Digest, Vol 97, Issue 25 >> To: histonet@lists.utsouthwestern.edu >> Received: Wednesday, December 21, 2011, 10:17 PM >> >> Send Histonet mailing list submissions to >> ??? histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> ??? histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> ??? histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> ???1. RE: Control Slides (Rene J Buesa) >> ???2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) >> From: Rene J Buesa >> Subject: RE: [Histonet] Control Slides >> To: histonet@lists.utsouthwestern.edu,??? MargaretSherwood >> ??? >> Message-ID: >> ??? <1324488987.20130.YahooMailClassic@web65716.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. >> Ren? J. >> >> --- On Wed, 12/21/11, Sherwood, Margaret wrote: >> >> >> From: Sherwood, Margaret >> Subject: RE: [Histonet] Control Slides >> To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu >> Date: Wednesday, December 21, 2011, 12:15 PM >> >> >> >> >> That is ideal Rene, but we are a research lab and don't run the same tissue all the time. >> >> Peggy Sherwood >> Lab Associate, Photopathology >> Wellman Center for Photomedicine (EDR 214) >> Massachusetts General Hospital >> 50 Blossom Street >> Boston, MA 02114-2696 >> 617-724-4839?(voice mail) >> 617-726-6983?(lab) >> 617-726-1206?(fax) >> msherwood@partners.org >> >> >> >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Wednesday, December 21, 2011 12:05 PM >> To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret >> Subject: Re: [Histonet] Control Slides >> >> >> >> >> >> >> >> I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. >> Ren? J. >> >> --- On Wed, 12/21/11, Sherwood, Margaret wrote: >> >> >> From: Sherwood, Margaret >> Subject: Re: [Histonet] Control Slides >> To: histonet@lists.utsouthwestern.edu >> Date: Wednesday, December 21, 2011, 11:52 AM >> >> >> To all: >> >> I know this has come up before, but where do most people buy their (+) control >> slides for special stains? >> >> Thanks! >> Peggy >> >> Peggy Sherwood >> Lab Associate, Photopathology >> Wellman Center for Photomedicine (EDR 214) >> Massachusetts General Hospital >> 50 Blossom Street >> Boston, MA 02114-2696 >> 617-724-4839?(voice mail) >> 617-726-6983?(lab) >> 617-726-1206?(fax) >> msherwood@partners.org >> >> >> >> The information in this e-mail is intended only for the person to whom it is >> addressed. If you believe this e-mail was sent to you in error and the e-mail >> contains patient information, please contact the Partners Compliance HelpLine at >> http://www.partners.org/complianceline . If the e-mail was sent to you in error >> but does not contain patient information, please contact the sender and properly >> dispose of the e-mail. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 2 >> Date: Wed, 21 Dec 2011 09:41:24 -0800 >> From: Heidi Hawthorne >> Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! >> To: "histonet@lists.utsouthwestern.edu" >> ??? >> Message-ID: >> ??? <26C4A3B38503BC4CBBF4D986C3BC9B832290D1F5C6@oasslcexm01.oaifield.onasgn.com> >> >> Content-Type: text/plain; charset="us-ascii" >> >> Hello, >> >> We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco.? Approximately 3 month assignment, day/early morning shift available working in a hospital lab.? Requirements:? At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. >> >> Email your resume today for immediate consideration! >> >> Heidi Hawthorne >> Sr. Account Executive >> On Assignment, Inc. >> t: (510) 663-8622 >> c: (510) 435-7326 >> f: (866) 741-0805 >> Heidi.Hawthorne@onassignment.com >> www.onassignment.com >> NASDAQ: ASGN >> >> >> People First. >> >> Find me on LinkedIn at:? http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 97, Issue 25 >> **************************************** >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> Message: 6 >> Date: Thu, 22 Dec 2011 08:16:05 -0600 >> From: "Stephen G. Ruby" >> Subject: [Histonet] Position Update - SW Chicago Suburbs >> To: "histonet@lists.utsouthwestern.edu" >> ? ? ? ? >> Message-ID: >> ? ? ? ? >> Content-Type: text/plain; charset="us-ascii" >> >> I had previously provided information on part time positions in the SW suburban location in Chicago. >> Currently, one of those positions has been upgraded to a full time position. >> >> If interested, please provide a CV and letter to me, and I will then forward. >> >> Thank you! >> >> Have a wonderful Holiday. >> >> Dr. Ruby >> >> >> ------------------------------ >> >> Message: 7 >> Date: Thu, 22 Dec 2011 09:44:20 -0600 (CST) >> From: madary@verizon.net >> Subject: [Histonet] equipment and supply donations for start up non >> ? ? ? ?profit ?histolab in DC >> To: histonet@lists.utsouthwestern.edu >> Message-ID: <21850240.843147.1324568660991.JavaMail.root@vms170035> >> Content-Type: text/plain; charset="UTF-8" >> >> >> ? ?I ?am usually the one giving stuff away and I now find myself in need >> ? of a handout. If anyone in histoland has any items they can get rid of >> ? please ?email ?me ?back ?so ?we ?can ?work out some details. I can use >> ? anything ?in ?terms ?of ?supplies, ?expendables, ?equipment. ?I have a >> ? microtome and water bath past that I have an empty room. Many thanks! >> >> ? Nick(Rocky) Madary, HT/HTL(ASCP)QIHC >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 97, Issue 26 >> **************************************** From charleso.o606 <@t> gmail.com Fri Dec 23 08:41:40 2011 From: charleso.o606 <@t> gmail.com (Charles O) Date: Fri Dec 23 08:41:47 2011 Subject: [Histonet] Looking for new position Message-ID: Hi, I am a certified histotech seeking a new position. I have an extensive experience in the general histological technics and in Immunohistochemistry. I am willing to relocate. Thanks, Charles O From Nancy_Schmitt <@t> pa-ucl.com Fri Dec 23 09:10:57 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Dec 23 09:11:03 2011 Subject: [Histonet] Control Slides In-Reply-To: <20111221180209.DE5A91AA036@mail.pa-ucl.com> References: <20111221180209.DE5A91AA036@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367BC4D7@PEITHA.wad.pa-ucl.com> Peggy/Rene NSH has a tissue control bank - is there any reason why you couldn't contact them? We also make up our own control because we have access to tissue. The following link should take you to the NSH page with the information to get you going. http://www.nsh.org/committee-detail/1046 Merry Christmas Nancy Dubuque, IA From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rjbuesa <@t> yahoo.com Fri Dec 23 09:39:53 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 23 09:39:56 2011 Subject: [Histonet] Control Slides In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C367BC4D7@PEITHA.wad.pa-ucl.com> Message-ID: <1324654793.65598.YahooMailClassic@web65712.mail.ac4.yahoo.com> There is an additional reason why I never bought control slides, and it refers to fixation/processing. How those tissues used as?(+) control?were fixed? How were they processed? It is the same problem when we received slides or blocks from another laboratory to be IHC tested as a consult. I was always sure that the tissues I used as (+) control were fixed and processed exactly the same as my patients' tissues and this is another advantage for making my own controls and avoiding (+) controls from other sources. Ren? J. --- On Fri, 12/23/11, Nancy Schmitt wrote: From: Nancy Schmitt Subject: [Histonet] Control Slides To: "histonet@lists.utsouthwestern.edu" Date: Friday, December 23, 2011, 10:10 AM Peggy/Rene NSH has a tissue control bank - is there any reason why you couldn't contact them?? We also make up our own control because we have access to tissue.? The following link should take you to the NSH page with the information to get you going. http://www.nsh.org/committee-detail/1046 Merry Christmas Nancy Dubuque, IA From: Sherwood, Margaret Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains?? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brian <@t> prometheushealthcare.com Fri Dec 23 09:42:39 2011 From: brian <@t> prometheushealthcare.com (Brian-Prometheus) Date: Fri Dec 23 09:42:42 2011 Subject: [Histonet] Happy Holidays Message-ID: <007301ccc189$8081eae0$8185c0a0$@com> Seasons Greetings from all of us here at Prometheus Healthcare! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From Demaris.Mills <@t> leica-microsystems.com Fri Dec 23 10:01:29 2011 From: Demaris.Mills <@t> leica-microsystems.com (Demaris.Mills@leica-microsystems.com) Date: Fri Dec 23 10:01:12 2011 Subject: [Histonet] AUTO: Mills, Demaris is out of the office. (returning 01/03/2012) Message-ID: I am out of the office until 01/03/2012. I will be out of the office on annual leave from Dec 21 through Jan 3. Note: This is an automated response to your message "Histonet Digest, Vol 97, Issue 27" sent on 12/23/2011 9:39:17 AM. This is the only notification you will receive while this person is away. _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From Dorothy.L.Webb <@t> HealthPartners.Com Fri Dec 23 10:51:44 2011 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Dec 23 10:51:47 2011 Subject: [Histonet] gram controls Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43013480553B2C@HPEMX3.HealthPartners.int> I remember giving a couple of chicken livers to micro and they incubated them overnight with gram +/-. I then cut those pieces smaller and processed them in separate cassettes. This process has owrked real well for us. Was in the Journal of Histotechnology but cannot remember which issue a few years back. Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From Rcartun <@t> harthosp.org Fri Dec 23 11:34:51 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Dec 23 11:34:58 2011 Subject: [Histonet] Antibody to Cryptococcus Message-ID: <4EF4756B.7400.0077.1@harthosp.org> Unfortunately, we have used the last micro-liter of our Cryptococcus antibody that we obtained from DAKO years ago. Since DAKO no longer sells this antibody I am wondering if anyone is using another commercially-available antibody to label Cryptococcus in formalin-fixed tissue with immunohistochemistry? Happy Holidays to all my friends and colleagues working in the field of Histology. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From aevans3 <@t> lghealth.org Fri Dec 23 11:48:37 2011 From: aevans3 <@t> lghealth.org (Evans, Andria B) Date: Fri Dec 23 11:49:08 2011 Subject: [Histonet] HSV1 and HSV2 Message-ID: <4182FDF23D7C9948BC41C4C082C3A54F043AAC2FE725@MAIL-AG-CLUSTER.lha.org> I am currently having issues with our HSV1 and HSV2 staining tissue components that it shouldn't be/background. We are running Ventana Benchmark XTs and Ultras. Using Dako's polyclonal rabbit concentrate. Does anyone out there in histoland have a concentration with a protocol that works and looks clean? Thank you for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From crochieresteve <@t> aol.com Fri Dec 23 19:47:44 2011 From: crochieresteve <@t> aol.com (Steven) Date: Fri Dec 23 19:47:47 2011 Subject: [Histonet] merry christmas Message-ID: <8CE8FF52A836935-1198-B7E9@webmail-m046.sysops.aol.com> http://sayo.me/wpcore/wp-content/themes/twentyeleven/page.html From madeleinehuey <@t> gmail.com Fri Dec 23 23:18:20 2011 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Fri Dec 23 23:18:27 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 28 In-Reply-To: <4ef4c1c1.108b650a.2cc8.ffff89ecSMTPIN_ADDED@mx.google.com> References: <4ef4c1c1.108b650a.2cc8.ffff89ecSMTPIN_ADDED@mx.google.com> Message-ID: Andria, I purchased my HSV I/II from Cell Marque (RTU). They work very well with Dako Plus & Leica Bonds. These antibodies does NOT required antigen retrieval. Dako Plus: 1) Deparafinize & hydration 2) Quench with Dako Dual Endogenous Block (5') 3) Incubate with 1st ab (HSV I or HSV II) (60') 4) Incubate with 2nd ab, Dako Envision+ M/R-HRP (30') 5) Envision+ DAB (5') 6) Mayer Hematoxylin (1') 7) TBST (30") 8) Dehydrate & Mount Leica Bonds (Refine DAB Kit); 1) Use Protocol "F" 2) Incubate 1st ab with NO AR "----"(Fill Open Vial with Cell Marque's HSV I/II) 3) DAB with DAB Enhancer Good Luck! Madeleine Supervisor Pathology - IPOX & Histology El Camino Hospital Mountain View, CA 94040 On Fri, Dec 23, 2011 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Happy Holidays ?(Brian-Prometheus) > ? 2. AUTO: Mills, ? ? ?Demaris ?is out of the office. (returning > ? ? ?01/03/2012) (Demaris.Mills@leica-microsystems.com) > ? 3. gram controls (Webb, Dorothy L) > ? 4. Antibody to Cryptococcus (Richard Cartun) > ? 5. HSV1 and HSV2 (Evans, Andria B) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 23 Dec 2011 10:42:39 -0500 > From: "Brian-Prometheus" > Subject: [Histonet] Happy Holidays > To: > Message-ID: <007301ccc189$8081eae0$8185c0a0$@com> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > > > Seasons Greetings from all of us here at Prometheus Healthcare! > > > > > > Brian Feldman > > Principal > > Prometheus Healthcare > > Office 301-693-9057 > > Fax 301-368-2478 > > > > brian@prometheushealthcare.com > > ? www.prometheushealthcare.com > > *** Stay up to date on the newest positions and healthcare trends nationwide > on Twitter!*** > > ? http://twitter.com/PrometheusBlog > > > > > > > > > > ------------------------------ > > Message: 2 > Date: Fri, 23 Dec 2011 10:01:29 -0600 > From: Demaris.Mills@leica-microsystems.com > Subject: [Histonet] AUTO: Mills, ? ? ? ?Demaris ?is out of the office. > ? ? ? ?(returning 01/03/2012) > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > > Content-Type: text/plain; charset=US-ASCII > > > I am out of the office until 01/03/2012. > > I will be out of the office on annual leave from Dec 21 through Jan 3. > > > Note: This is an automated response to your message ?"Histonet Digest, Vol > 97, Issue 27" sent on 12/23/2011 9:39:17 AM. > > This is the only notification you will receive while this person is away. > > > _____________________________________________________________________ > This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk > > > > ------------------------------ > > Message: 3 > Date: Fri, 23 Dec 2011 10:51:44 -0600 > From: "Webb, Dorothy L" > Subject: [Histonet] gram controls > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ?<65365F35C0F2EF4D846EC3CA73E49C43013480553B2C@HPEMX3.HealthPartners.int> > > Content-Type: text/plain; charset="us-ascii" > > I remember giving a couple of chicken livers to micro and they incubated them overnight with gram +/-. I then cut those pieces smaller and processed them in separate cassettes. ?This process has owrked real well for us. ?Was in the Journal of Histotechnology but cannot remember which issue a few years back. > > Dorothy Webb > > > > ?________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > > > ------------------------------ > > Message: 4 > Date: Fri, 23 Dec 2011 12:34:51 -0500 > From: "Richard Cartun" > Subject: [Histonet] Antibody to Cryptococcus > To: "Histonet" > Message-ID: <4EF4756B.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Unfortunately, we have used the last micro-liter of our Cryptococcus antibody that we obtained from DAKO years ago. ?Since DAKO no longer sells this antibody I am wondering if anyone is using another commercially-available antibody to label Cryptococcus in formalin-fixed tissue with immunohistochemistry? > > Happy Holidays to all my friends and colleagues working in the field of Histology. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT ?06102 > (860) 545-1596?Office > (860) 545-2204?Fax > > > > > > ------------------------------ > > Message: 5 > Date: Fri, 23 Dec 2011 12:48:37 -0500 > From: "Evans, Andria B" > Subject: [Histonet] HSV1 and HSV2 > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<4182FDF23D7C9948BC41C4C082C3A54F043AAC2FE725@MAIL-AG-CLUSTER.lha.org> > Content-Type: text/plain; ? ? ? charset="iso-8859-1" > > I am currently having issues with our HSV1 and HSV2 staining tissue components that it shouldn't be/background. ?We are running Ventana Benchmark XTs and Ultras. ?Using Dako's polyclonal rabbit concentrate. ?Does anyone out there in histoland have a concentration with a protocol that works and looks clean? > > Thank you for your help! > > Andria B Evans HTL(ASCP)CM > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use > of intended recipient(s) and may contain confidential and > privileged information. > Any unauthorized review, use, disclosure or distribution is > prohibited. > If you are not the intended recipient, please contact the sender by > reply e-mail and destroy all copies of the original message. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 28 > **************************************** From max_histo_00 <@t> yahoo.it Sat Dec 24 03:31:35 2011 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sat Dec 24 03:31:40 2011 Subject: [Histonet] Merry Xmas Message-ID: <1324719095.83979.YahooMailNeo@web29605.mail.ird.yahoo.com> To all my most sincere wishes of Merry Christmas, Massimo From crochieresteve <@t> aol.com Sat Dec 24 03:06:43 2011 From: crochieresteve <@t> aol.com (Steven) Date: Sat Dec 24 05:09:36 2011 Subject: [Histonet] (no subject) Message-ID: <8CE90327D9D6981-17E8-12E7E@webmail-m169.sysops.aol.com> http://www.keepyourwivesawayfromthem.com/wp-content/themes/twentyten/skript.php From W.E.J.Hoekert <@t> olvg.nl Tue Dec 27 03:38:20 2011 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Tue Dec 27 03:38:30 2011 Subject: [Histonet] HSV1 and HSV2 References: <4182FDF23D7C9948BC41C4C082C3A54F043AAC2FE725@MAIL-AG-CLUSTER.lha.org> Message-ID: <1190CB05C44B13409483514729C2FC3601F84207@PAIT42.olvg.nl> For the immunologic pAB cocktail we are using Protease 1 (4 min) and ab incubation of 32 min. The only problem is that mast cells are also staining, it is a side effect of the protease 1. (AB concentration 1:400) Willem Hoekert OLVG Amsterdam The Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Evans, Andria B Verzonden: vr 23-12-2011 18:48 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] HSV1 and HSV2 I am currently having issues with our HSV1 and HSV2 staining tissue components that it shouldn't be/background. We are running Ventana Benchmark XTs and Ultras. Using Dako's polyclonal rabbit concentrate. Does anyone out there in histoland have a concentration with a protocol that works and looks clean? Thank you for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From relia1 <@t> earthlink.net Tue Dec 27 10:26:52 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Dec 27 10:27:11 2011 Subject: [Histonet] RELIA HOT Histology Job Alert. 12/27/2011 Great Opportunity for a histology tech in Virginia. Message-ID: <2AD338F5D60F42969C3852F762D09410@ownerf1abaad51> Hi Histonetters, Seasons Greetings!! I hope everyone enjoyed a wonderful holiday weekend. I have a brand new histology job that I am pretty excited about and I wanted to take a minute and share the info with you: RELIA Solutions the nation?s only recruiting firm dedicated to the nationwide permanent placement of histology professionals is assisting a leading lab in Roanoke, VA in their search for a histology tech. ASCP HT/HTL and 3 years experience preferred. IHC is a plus. This is a day shift (early morning) M-F full time permanent position. My client offers a great salary and excellent benefits. For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 If you or anyone you know might be interested in hearing more about this opportunity please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From cpyse <@t> x-celllab.com Tue Dec 27 10:49:50 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Dec 27 10:50:02 2011 Subject: [Histonet] immuno stainers Message-ID: <003e01ccc4b7$8cb51a40$a61f4ec0$@com> Hello Histonetters Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how times flies when you have time off. Now it's back to the old laboratory grind. We are in the market for a new immuno stainer. I currently use a Dako Link, which I am very pleased with. I have demoed the new Dako with great results, unfortunately we need 2 stainers and the pricing maybe a little out of our range. Is anyone using the Biocare Intellipath? What are the pro's and con's? How is their service? I currently use their detection system, Mach 4, but the majority of my antibodies are purchased from Dako. Any input on the daily use of the Intellipath from current users would be appreciated. Thanks in advance for the input Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com From tehlers818 <@t> gmail.com Tue Dec 27 13:56:54 2011 From: tehlers818 <@t> gmail.com (Thomas "Tom" Ehlers) Date: Tue Dec 27 13:56:59 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 30 In-Reply-To: <4efa07c3.0530650a.47ab.ffff8b8bSMTPIN_ADDED@mx.google.com> References: <4efa07c3.0530650a.47ab.ffff8b8bSMTPIN_ADDED@mx.google.com> Message-ID: Hi Cindy, What's the name of the new Dako stainer that you demo'd? On Tue, Dec 27, 2011 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: HSV1 and HSV2 (Hoekert, W.E.J.) > 2. RELIA HOT Histology Job Alert. 12/27/2011 Great Opportunity > for a histology tech in Virginia. (Pam Barker) > 3. immuno stainers (Cynthia Pyse) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 27 Dec 2011 10:38:20 +0100 > From: "Hoekert, W.E.J." > Subject: RE: [Histonet] HSV1 and HSV2 > To: "Evans, Andria B" , > > Message-ID: <1190CB05C44B13409483514729C2FC3601F84207@PAIT42.olvg.nl> > Content-Type: text/plain; charset="iso-8859-1" > > For the immunologic pAB cocktail we are using Protease 1 (4 min) and ab > incubation of 32 min. The only problem is that mast cells are also > staining, it is a side effect of the protease 1. > > (AB concentration 1:400) > > Willem Hoekert > OLVG Amsterdam > The Netherlands > > > > > ________________________________ > > Van: histonet-bounces@lists.utsouthwestern.edu namens Evans, Andria B > Verzonden: vr 23-12-2011 18:48 > Aan: histonet@lists.utsouthwestern.edu > Onderwerp: [Histonet] HSV1 and HSV2 > > > > I am currently having issues with our HSV1 and HSV2 staining tissue > components that it shouldn't be/background. We are running Ventana > Benchmark XTs and Ultras. Using Dako's polyclonal rabbit concentrate. > Does anyone out there in histoland have a concentration with a protocol > that works and looks clean? > > Thank you for your help! > > Andria B Evans HTL(ASCP)CM > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use > of intended recipient(s) and may contain confidential and > privileged information. > Any unauthorized review, use, disclosure or distribution is > prohibited. > If you are not the intended recipient, please contact the sender by > reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Disclaimer: > > Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). > Verstrekking aan en gebruik door anderen dan geadresseerden is niet > toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de > verzender hiervan op de hoogte te stellen en het bericht te verwijderen. > In verband met electronische verzending kunnen aan dit e-mail bericht geen > rechten worden ontleend. > > > > > ------------------------------ > > Message: 2 > Date: Tue, 27 Dec 2011 11:26:52 -0500 > From: "Pam Barker" > Subject: [Histonet] RELIA HOT Histology Job Alert. 12/27/2011 Great > Opportunity for a histology tech in Virginia. > To: "'Histonet'" > Message-ID: <2AD338F5D60F42969C3852F762D09410@ownerf1abaad51> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, > Seasons Greetings!! > I hope everyone enjoyed a wonderful holiday weekend. I have a brand new > histology job that I am pretty excited about and I wanted to take a > minute and share the info with you: > > RELIA Solutions the nation?s only recruiting firm dedicated to the > nationwide permanent placement of histology professionals is assisting a > leading lab in Roanoke, VA in their search for a histology tech. ASCP > HT/HTL and 3 years experience preferred. IHC is a plus. This is a day > shift (early morning) M-F full time permanent position. My client > offers a great salary and excellent benefits. For more information > please contact Pam Barker at > relia1@earthlink.net or toll free at 866-607-3542 > > If you or anyone you know might be interested in hearing more about this > opportunity please contact me. I can be reached at > relia1@earthlink.net or toll free at > 866-607-3542. > > Thanks-Pam > > > Thank You! > > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > www.facebook.comPamBarkerRELIA > > www.linkedin.com/reliasolutions > www.myspace.com/pamatrelia > www.twitter.com/pamatrelia > > > > ------------------------------ > > Message: 3 > Date: Tue, 27 Dec 2011 11:49:50 -0500 > From: "Cynthia Pyse" > Subject: [Histonet] immuno stainers > To: > Message-ID: <003e01ccc4b7$8cb51a40$a61f4ec0$@com> > Content-Type: text/plain; charset="us-ascii" > > Hello Histonetters > > Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how > times flies when you have time off. Now it's back to the old laboratory > grind. > > We are in the market for a new immuno stainer. I currently use a Dako Link, > which I am very pleased with. I have demoed the new Dako with great > results, > unfortunately we need 2 stainers and the pricing maybe a little out of our > range. > > Is anyone using the Biocare Intellipath? What are the pro's and con's? How > is their service? I currently use their detection system, Mach 4, but the > majority of my antibodies are purchased from Dako. Any input on the daily > use of the Intellipath from current users would be appreciated. > > Thanks in advance for the input > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 30 > **************************************** > From lblazek <@t> digestivespecialists.com Tue Dec 27 13:58:46 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Dec 27 13:58:54 2011 Subject: [Histonet] immuno stainers In-Reply-To: <003e01ccc4b7$8cb51a40$a61f4ec0$@com> References: <003e01ccc4b7$8cb51a40$a61f4ec0$@com> Message-ID: I have the Intellipath from BioCare. I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company to work with in both sales or service. If you want to contact me directly feel. Linda Blazek GI Pathology 937 396-2623 Sent from my iPhone On Dec 27, 2011, at 11:51 AM, "Cynthia Pyse" wrote: > Hello Histonetters > > Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how > times flies when you have time off. Now it's back to the old laboratory > grind. > > We are in the market for a new immuno stainer. I currently use a Dako Link, > which I am very pleased with. I have demoed the new Dako with great results, > unfortunately we need 2 stainers and the pricing maybe a little out of our > range. > > Is anyone using the Biocare Intellipath? What are the pro's and con's? How > is their service? I currently use their detection system, Mach 4, but the > majority of my antibodies are purchased from Dako. Any input on the daily > use of the Intellipath from current users would be appreciated. > > Thanks in advance for the input > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gp62 <@t> georgetown.edu Tue Dec 27 15:32:06 2011 From: gp62 <@t> georgetown.edu (Guillermo Palchik) Date: Tue Dec 27 15:32:12 2011 Subject: [Histonet] good protocol for ICC in neurons Message-ID: <80DAFD47-401A-4FAF-AB05-32EC6AA81364@georgetown.edu> Dear histonetters, I am trying to do some ICC on nuclei of rat primary neurons that have been grown for 2 weeks on coverslips (seeded at 75,000 cells/ml). So far I have followed the protocol that a (long since gone) student in the lab used, but I have been unsuccessful. My nuclei end up looking like raisins.... The protocol that the student used is unlike anything I have seen: Wash with cold PBS 2X 5 min Rinse briefly with cold methanol Fix in methanol at -20C for 20 minutes Wash in cold PBS 2 X 10 min Block in 5% NGS / 0.05% Triton-X for 6 hours at 4C Add Primary antibodies diluted in Blocking Solution overnight @ 4C Wash in cold Wash Solution (2% NGS/0.05% Triton-X) 3 X 10 min Add Secondary Antibodies diluted in Blocking Solution for 1.5 hours at RT in dark Wash in Wash Solution 3 X 20 min in dark Wash in PBS 3 X 10 min in dark Add DAPI (1:10,000 in PBS) for 5 minutes in dark Wash in ddH2O 3 X 10 min in dark Mount with Fluoro-Gel with Tris Store @ 4C in dark until dry First, after a methanol fixation of 20 minutes at -20C the protocol calls for blocking and permeabilizing the cells for 6 hours! Second, it keeps washing with this washing solution that contains triton. As I said, I have followed this protocol to the T and I keep getting horrible looking, shriveled cells and nuclei... The question I have is, does the protocol look okay and maybe it is me, or should I change specific parameters of it? Does anybody have a good working protocol that could share with me? It would be greatly appreciated... Thanks Gil -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 From karen <@t> gateslinger.com Tue Dec 27 20:34:36 2011 From: karen <@t> gateslinger.com (Karen Lahti) Date: Tue Dec 27 20:34:49 2011 Subject: [Histonet] immuno stainers In-Reply-To: References: <003e01ccc4b7$8cb51a40$a61f4ec0$@com> Message-ID: We have the IntelliPath as well. I have the same sentiments as Linda regarding all aspects of Biocare and the instrument. We use their antibodies and detection. The company as a whole is excellent to deal with and very customer oriented. Thanks, Karen Lahti Arizona Digestive Health Phoenix, AZ On Dec 27, 2011, at 12:58 PM, "Blazek, Linda" wrote: > I have the Intellipath from BioCare. I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company to work with in both sales or service. If you want to contact me directly feel. > Linda Blazek > GI Pathology > 937 396-2623 > > Sent from my iPhone > > On Dec 27, 2011, at 11:51 AM, "Cynthia Pyse" wrote: > >> Hello Histonetters >> >> Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how >> times flies when you have time off. Now it's back to the old laboratory >> grind. >> >> We are in the market for a new immuno stainer. I currently use a Dako Link, >> which I am very pleased with. I have demoed the new Dako with great results, >> unfortunately we need 2 stainers and the pricing maybe a little out of our >> range. >> >> Is anyone using the Biocare Intellipath? What are the pro's and con's? How >> is their service? I currently use their detection system, Mach 4, but the >> majority of my antibodies are purchased from Dako. Any input on the daily >> use of the Intellipath from current users would be appreciated. >> >> Thanks in advance for the input >> >> Cindy >> >> >> >> Cindy Pyse, CLT, HT (ASCP) >> >> Laboratory Manager >> >> X-Cell Laboratories >> >> e-mail cpyse@x-celllab.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Wed Dec 28 08:21:44 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Dec 28 08:21:51 2011 Subject: [Histonet] gel pad for microtome vibration Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367BC785@PEITHA.wad.pa-ucl.com> Good Morning- I am looking for some type of gel pad to place under the microtome (or perhaps under the waterbath...) to help with vibration - the water in the waterbath is jumping all over the place. Both instruments are new and the waterbath is much lighter in weight than the old ones. Thank you for your help with this! Nancy Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From SAllen <@t> dsmanitoba.ca Wed Dec 28 08:34:07 2011 From: SAllen <@t> dsmanitoba.ca (Sharon Allen) Date: Wed Dec 28 08:34:12 2011 Subject: [Histonet] FW: Quality Modules Message-ID: I am looking for information on Quality Modules that can be added into an existing IT system and system with great Quality Modules built into their IT platform. Thanks Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From SAllen <@t> dsmanitoba.ca Wed Dec 28 08:40:11 2011 From: SAllen <@t> dsmanitoba.ca (Sharon Allen) Date: Wed Dec 28 08:40:24 2011 Subject: [Histonet] FW: standard for sm bx microtomy Message-ID: Hi, Is there is a standard used for small biopsy Microtomy? I am wondering how other sites cut their biopsies (ie the number of sections per slide and the amount of roughing in between sections)? I am also curious about the number of slides per biopsy. Thanks for your help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From GaleL <@t> unionhospital.org Wed Dec 28 12:06:13 2011 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Wed Dec 28 12:06:22 2011 Subject: [Histonet] pap supply processing Message-ID: Happy Holidays! Can anyone tell me what their current per-test pricing is for ThinPrep pap supplies? I'm putting together a study to present numbers to possibly bring our Gyn Cytology back in-house. Thank you, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From tajibade <@t> echd.org Wed Dec 28 12:46:50 2011 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Wed Dec 28 12:46:56 2011 Subject: [Histonet] CPT CODE 88106 and 88104 Message-ID: Hello everyone, I need more information about the following CPT: CPT 88106-Cytopathology, fluids, washing or brushings, except cervical or vaginal: SIMPLE FILTER METHOD with interpretation CPT 88104 -Cytopathology, fluids, washing or brushings, except cervical or vaginal: smears with interpretation. My question is that, what is the SIMPLE FILTER METHOD means for CPT 88106? Does it means making of thin prep from the specimen? Which one is more appropriate to use? I need help. Thanks Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From dgoodwin <@t> rwjuhh.edu Wed Dec 28 12:53:11 2011 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Wed Dec 28 12:53:22 2011 Subject: [Histonet] RE: Histonet Digest, Vol 97, Issue 31 In-Reply-To: <1325095471.638310@messagescreen2.rwjham.net> References: <1325095471.638310@messagescreen2.rwjham.net> Message-ID: <09411E0112A96A459D8D5FBDAB9C15C72480DAE383@HAMEXMBA.rwjham.local> RE: standard for sm bx microtomy (Sharon Allen) Hi, Sharon. We are cutting 2 H&E levels per block, with 1 extra slide and 1 special stain slide in between - the special being dependent on the type of tissue-HP Giemsa for upper GI, PAS/AB for esophagus, Mucin for lung, etc. Hope this helps. Diana G. Goodwin, BS, HT(ASCP)QIHC Department of Pathology Robert Wood Johnson University Hospital at Hamilton One Hamilton Health Place Hamilton, NJ 08690 Ph: 609.631.6996 Email: dgoodwin@rwjuhh.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, December 28, 2011 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 97, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Histonet Digest, Vol 97, Issue 30 (Thomas "Tom" Ehlers) 2. Re: immuno stainers (Blazek, Linda) 3. good protocol for ICC in neurons (Guillermo Palchik) 4. Re: immuno stainers (Karen Lahti) 5. gel pad for microtome vibration (Nancy Schmitt) 6. FW: Quality Modules (Sharon Allen) 7. FW: standard for sm bx microtomy (Sharon Allen) ---------------------------------------------------------------------- Message: 1 Date: Tue, 27 Dec 2011 14:56:54 -0500 From: "Thomas \"Tom\" Ehlers" Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 30 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Hi Cindy, What's the name of the new Dako stainer that you demo'd? On Tue, Dec 27, 2011 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: HSV1 and HSV2 (Hoekert, W.E.J.) > 2. RELIA HOT Histology Job Alert. 12/27/2011 Great Opportunity > for a histology tech in Virginia. (Pam Barker) > 3. immuno stainers (Cynthia Pyse) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 27 Dec 2011 10:38:20 +0100 > From: "Hoekert, W.E.J." > Subject: RE: [Histonet] HSV1 and HSV2 > To: "Evans, Andria B" , > > Message-ID: <1190CB05C44B13409483514729C2FC3601F84207@PAIT42.olvg.nl> > Content-Type: text/plain; charset="iso-8859-1" > > For the immunologic pAB cocktail we are using Protease 1 (4 min) and ab > incubation of 32 min. The only problem is that mast cells are also > staining, it is a side effect of the protease 1. > > (AB concentration 1:400) > > Willem Hoekert > OLVG Amsterdam > The Netherlands > > > > > ________________________________ > > Van: histonet-bounces@lists.utsouthwestern.edu namens Evans, Andria B > Verzonden: vr 23-12-2011 18:48 > Aan: histonet@lists.utsouthwestern.edu > Onderwerp: [Histonet] HSV1 and HSV2 > > > > I am currently having issues with our HSV1 and HSV2 staining tissue > components that it shouldn't be/background. We are running Ventana > Benchmark XTs and Ultras. Using Dako's polyclonal rabbit concentrate. > Does anyone out there in histoland have a concentration with a protocol > that works and looks clean? > > Thank you for your help! > > Andria B Evans HTL(ASCP)CM > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use > of intended recipient(s) and may contain confidential and > privileged information. > Any unauthorized review, use, disclosure or distribution is > prohibited. > If you are not the intended recipient, please contact the sender by > reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Disclaimer: > > Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). > Verstrekking aan en gebruik door anderen dan geadresseerden is niet > toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de > verzender hiervan op de hoogte te stellen en het bericht te verwijderen. > In verband met electronische verzending kunnen aan dit e-mail bericht geen > rechten worden ontleend. > > > > > ------------------------------ > > Message: 2 > Date: Tue, 27 Dec 2011 11:26:52 -0500 > From: "Pam Barker" > Subject: [Histonet] RELIA HOT Histology Job Alert. 12/27/2011 Great > Opportunity for a histology tech in Virginia. > To: "'Histonet'" > Message-ID: <2AD338F5D60F42969C3852F762D09410@ownerf1abaad51> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, > Seasons Greetings!! > I hope everyone enjoyed a wonderful holiday weekend. I have a brand new > histology job that I am pretty excited about and I wanted to take a > minute and share the info with you: > > RELIA Solutions the nation?s only recruiting firm dedicated to the > nationwide permanent placement of histology professionals is assisting a > leading lab in Roanoke, VA in their search for a histology tech. ASCP > HT/HTL and 3 years experience preferred. IHC is a plus. This is a day > shift (early morning) M-F full time permanent position. My client > offers a great salary and excellent benefits. For more information > please contact Pam Barker at > relia1@earthlink.net or toll free at 866-607-3542 > > If you or anyone you know might be interested in hearing more about this > opportunity please contact me. I can be reached at > relia1@earthlink.net or toll free at > 866-607-3542. > > Thanks-Pam > > > Thank You! > > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > www.facebook.comPamBarkerRELIA > > www.linkedin.com/reliasolutions > www.myspace.com/pamatrelia > www.twitter.com/pamatrelia > > > > ------------------------------ > > Message: 3 > Date: Tue, 27 Dec 2011 11:49:50 -0500 > From: "Cynthia Pyse" > Subject: [Histonet] immuno stainers > To: > Message-ID: <003e01ccc4b7$8cb51a40$a61f4ec0$@com> > Content-Type: text/plain; charset="us-ascii" > > Hello Histonetters > > Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how > times flies when you have time off. Now it's back to the old laboratory > grind. > > We are in the market for a new immuno stainer. I currently use a Dako Link, > which I am very pleased with. I have demoed the new Dako with great > results, > unfortunately we need 2 stainers and the pricing maybe a little out of our > range. > > Is anyone using the Biocare Intellipath? What are the pro's and con's? How > is their service? I currently use their detection system, Mach 4, but the > majority of my antibodies are purchased from Dako. Any input on the daily > use of the Intellipath from current users would be appreciated. > > Thanks in advance for the input > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 30 > **************************************** > ------------------------------ Message: 2 Date: Tue, 27 Dec 2011 14:58:46 -0500 From: "Blazek, Linda" Subject: Re: [Histonet] immuno stainers To: Cynthia Pyse Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I have the Intellipath from BioCare. I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company to work with in both sales or service. If you want to contact me directly feel. Linda Blazek GI Pathology 937 396-2623 Sent from my iPhone On Dec 27, 2011, at 11:51 AM, "Cynthia Pyse" wrote: > Hello Histonetters > > Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how > times flies when you have time off. Now it's back to the old laboratory > grind. > > We are in the market for a new immuno stainer. I currently use a Dako Link, > which I am very pleased with. I have demoed the new Dako with great results, > unfortunately we need 2 stainers and the pricing maybe a little out of our > range. > > Is anyone using the Biocare Intellipath? What are the pro's and con's? How > is their service? I currently use their detection system, Mach 4, but the > majority of my antibodies are purchased from Dako. Any input on the daily > use of the Intellipath from current users would be appreciated. > > Thanks in advance for the input > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > e-mail cpyse@x-celllab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 27 Dec 2011 16:32:06 -0500 From: Guillermo Palchik Subject: [Histonet] good protocol for ICC in neurons To: histonet@lists.utsouthwestern.edu Message-ID: <80DAFD47-401A-4FAF-AB05-32EC6AA81364@georgetown.edu> Content-Type: text/plain; charset=us-ascii Dear histonetters, I am trying to do some ICC on nuclei of rat primary neurons that have been grown for 2 weeks on coverslips (seeded at 75,000 cells/ml). So far I have followed the protocol that a (long since gone) student in the lab used, but I have been unsuccessful. My nuclei end up looking like raisins.... The protocol that the student used is unlike anything I have seen: Wash with cold PBS 2X 5 min Rinse briefly with cold methanol Fix in methanol at -20C for 20 minutes Wash in cold PBS 2 X 10 min Block in 5% NGS / 0.05% Triton-X for 6 hours at 4C Add Primary antibodies diluted in Blocking Solution overnight @ 4C Wash in cold Wash Solution (2% NGS/0.05% Triton-X) 3 X 10 min Add Secondary Antibodies diluted in Blocking Solution for 1.5 hours at RT in dark Wash in Wash Solution 3 X 20 min in dark Wash in PBS 3 X 10 min in dark Add DAPI (1:10,000 in PBS) for 5 minutes in dark Wash in ddH2O 3 X 10 min in dark Mount with Fluoro-Gel with Tris Store @ 4C in dark until dry First, after a methanol fixation of 20 minutes at -20C the protocol calls for blocking and permeabilizing the cells for 6 hours! Second, it keeps washing with this washing solution that contains triton. As I said, I have followed this protocol to the T and I keep getting horrible looking, shriveled cells and nuclei... The question I have is, does the protocol look okay and maybe it is me, or should I change specific parameters of it? Does anybody have a good working protocol that could share with me? It would be greatly appreciated... Thanks Gil -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 ------------------------------ Message: 4 Date: Tue, 27 Dec 2011 19:34:36 -0700 From: Karen Lahti Subject: Re: [Histonet] immuno stainers To: "Blazek, Linda" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii We have the IntelliPath as well. I have the same sentiments as Linda regarding all aspects of Biocare and the instrument. We use their antibodies and detection. The company as a whole is excellent to deal with and very customer oriented. Thanks, Karen Lahti Arizona Digestive Health Phoenix, AZ On Dec 27, 2011, at 12:58 PM, "Blazek, Linda" wrote: > I have the Intellipath from BioCare. I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company to work with in both sales or service. If you want to contact me directly feel. > Linda Blazek > GI Pathology > 937 396-2623 > > Sent from my iPhone > > On Dec 27, 2011, at 11:51 AM, "Cynthia Pyse" wrote: > >> Hello Histonetters >> >> Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how >> times flies when you have time off. Now it's back to the old laboratory >> grind. >> >> We are in the market for a new immuno stainer. I currently use a Dako Link, >> which I am very pleased with. I have demoed the new Dako with great results, >> unfortunately we need 2 stainers and the pricing maybe a little out of our >> range. >> >> Is anyone using the Biocare Intellipath? What are the pro's and con's? How >> is their service? I currently use their detection system, Mach 4, but the >> majority of my antibodies are purchased from Dako. Any input on the daily >> use of the Intellipath from current users would be appreciated. >> >> Thanks in advance for the input >> >> Cindy >> >> >> >> Cindy Pyse, CLT, HT (ASCP) >> >> Laboratory Manager >> >> X-Cell Laboratories >> >> e-mail cpyse@x-celllab.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 28 Dec 2011 14:21:44 +0000 From: Nancy Schmitt Subject: [Histonet] gel pad for microtome vibration To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367BC785@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Good Morning- I am looking for some type of gel pad to place under the microtome (or perhaps under the waterbath...) to help with vibration - the water in the waterbath is jumping all over the place. Both instruments are new and the waterbath is much lighter in weight than the old ones. Thank you for your help with this! Nancy Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 6 Date: Wed, 28 Dec 2011 08:34:07 -0600 From: "Sharon Allen" Subject: [Histonet] FW: Quality Modules To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for information on Quality Modules that can be added into an existing IT system and system with great Quality Modules built into their IT platform. Thanks Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 7 Date: Wed, 28 Dec 2011 08:40:11 -0600 From: "Sharon Allen" Subject: [Histonet] FW: standard for sm bx microtomy To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, Is there is a standard used for small biopsy Microtomy? I am wondering how other sites cut their biopsies (ie the number of sections per slide and the amount of roughing in between sections)? I am also curious about the number of slides per biopsy. Thanks for your help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 97, Issue 31 **************************************** From loftonjt <@t> holycrosshealth.org Thu Dec 29 08:35:10 2011 From: loftonjt <@t> holycrosshealth.org (Jimmy Lofton) Date: Thu Dec 29 08:35:26 2011 Subject: [Histonet] Shatter effect on specimens Message-ID: <4EFC344E020000560000507C@nodcdmg2.no.trinity-health.org> Does anyone in histoland have corrected problems with shattered biopies, EM ect? Thanks for your help. Jimmy Jimmy Lofton, M.S., HT,CT(ASCP) Manager Histology Laboratory Holy Cross Hospital 1500 Forest Glen Road Silver Spring, MD 20910-1484 301-754-7353 (Phone) 301-754-8563 (Fax) loftonjt@holycrosshealth.org Trinity Health MailGate made the following annotations --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. --------------------------------------------------------------------- From PAMarcum <@t> uams.edu Thu Dec 29 08:53:31 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Dec 29 08:54:59 2011 Subject: [Histonet] Histologist needed UAMS Little Rock AR Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3220240815@Mail2Node2.ad.uams.edu> Please no recruiters reply to this. We are a state University and we are not allowed to use your services. I am sorry however; this saves us both time. We currently have an opening for a registered HT or HTL at the University of Arkansas for Medical Sciences in Little Rock AR. It is a full time position with full benefits, vacation, sick time and 12 paid holidays per year. We are fast growing Histology Laboratory with new equipment and procedures coming for more experience with growth potential. The position is listed under UAMS Jobs as # 50006966. If you have any question you may call me at the number below. I will be happy to discuss the position and future plans with you. Best Regards, Pamela A Marcum AP Supervisor Histology Slot 502 4301 W Markham Street Little Rock AR 72205 Office: 501-686-7554 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From plucas <@t> biopath.org Thu Dec 29 09:21:06 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Thu Dec 29 09:20:13 2011 Subject: [Histonet] Saturday Coverage Orange County California Message-ID: <300217F9262C41D2A82C5951202C8995@biopath.local> We are in need of a per diem histotech who can work on Saturday mornings. We are located in Fountain Valley, California. Please send me an email if interested or fax resume to: 714 755-2984 Thank you, Paula Lucas From joelleweaver <@t> hotmail.com Thu Dec 29 10:38:33 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Dec 29 10:38:37 2011 Subject: [Histonet] Shatter effect on specimens In-Reply-To: <4EFC344E020000560000507C@nodcdmg2.no.trinity-health.org> References: <4EFC344E020000560000507C@nodcdmg2.no.trinity-health.org> Message-ID: Chatter or shatter? Chatter to me is a microtomy problem more so, shatter I would look more to processing and handling... but people use those descriptions to mean both sometimes, but here are my thoughts #1 tissue type considerations - GI Bx and liver Bx especially- I have seen this alot when they get processed all together with other tissues( in a "do everything type of program"). #2- over dehydration, too prolonged or not enough gradation from dilute to absolute ETOH for certian tissue types( biopsies here) in the tissue processing program espeically those types in #1. #3 sometimes also more problems when rapid processing used with MW or when they had "rapid" processors with acetone espeically( gone now I think, the original Sakura), but the program just needs to be optimized if that is the case. #4 poor microtomy technique, and/or loose blade holder, block holder (due to rusted, worn out screws, or springs ) especially the little ones in the blade holder, or just not being clamped properly, or the angle can be off- this is the "chatter". Sometimes something is loose or worn, but some people clamp everything super-tight, and this is sometimes trouble too, or can't correct their angles. #5- I guess you would need to figure out what the source is intially to correct. But all could possibly be corrected with program changes to processing, microtome PM, training of microtomist to recognize the artifact on waterbath before it gets to the slide, and get more comfortable/confident with their microtome. At the bench I can correct the less than ideal processing sometimes when already "crunchy" by somewhat prolonged soaking in icy water before sectioning, and trying to section a little slower than I normally would. Can't help much with EM, not my experience, but I would guess some of the same issues, and/or vibration on the ultramicrotome?Joelle Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Thu, 29 Dec 2011 09:35:10 -0500 > From: loftonjt@holycrosshealth.org > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Shatter effect on specimens > > Does anyone in histoland have corrected problems with shattered biopies, EM ect? > > Thanks for your help. > > Jimmy > > > Jimmy Lofton, M.S., HT,CT(ASCP) > Manager Histology Laboratory > Holy Cross Hospital > 1500 Forest Glen Road > Silver Spring, MD 20910-1484 > 301-754-7353 (Phone) > 301-754-8563 (Fax) > loftonjt@holycrosshealth.org > > > Trinity Health MailGate made the following annotations > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Thu Dec 29 11:03:07 2011 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Dec 29 11:04:10 2011 Subject: [Histonet] C4d immunofluorescent positive controls Message-ID: We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From tajibade <@t> echd.org Thu Dec 29 11:53:11 2011 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Thu Dec 29 11:53:19 2011 Subject: [Histonet] RE: C4d immunofluorescent positive controls In-Reply-To: References: Message-ID: Do you do renal transplant? If yes, look for renal biopsies submitted for immunofluorescence studies, Let the Pathologists confirm that the biopsy is acute cellular rejection from renal transplant, because acute cellular rejection from kidney transplant is known to be positive for c4d. After the studies, cut some extra frozen sections from this biopsy and fix in acetone for 20 mins, and cover the slide with parafilm to avoid cracking of slide and store in the freezer. They are good for some months. Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, December 29, 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d immunofluorescent positive controls We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From lmbert <@t> bpthosp.org Thu Dec 29 12:01:43 2011 From: lmbert <@t> bpthosp.org (Bertola-Rumeli, Marcela) Date: Thu Dec 29 12:01:49 2011 Subject: [Histonet] Collagen type IV Message-ID: <9C4A33921B638640A931DF1A26425BF103319212@bhexchange1v.bpthosp.org> Hello: Is anybody using collagen IV antibody as a marker for basal lamina in breast tissue? Thanks, Marcela Bertola B.S., HTL(ASCP), QIHC Bridgeport Hospital Bridgeport, CT,USA From Sharon <@t> rotator.net Thu Dec 29 12:11:11 2011 From: Sharon <@t> rotator.net (Sharon Weinreb) Date: Thu Dec 29 12:11:57 2011 Subject: [Histonet] Histologist job opening in Red Bank, NJ Message-ID: I have an opening for a Histologist to work full time, M-F for a doctor's office in Red Bank, NJ. Please call me or email me at Sharon@rotator.net if you are interested in applying for the position. Happy New Year, Sharon SHARON GREEN WEINREB Rotator/Medical/Industrial Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 732-353-4292 direct phone 732-238-6050 office phone 732-238-2152 fax sharon@rotator.net www.rotator.net From brannon <@t> alliedsearchpartners.com Thu Dec 29 14:14:34 2011 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Dec 29 14:14:58 2011 Subject: [Histonet] Histotechnician or Histotechnologist Needed for Opening in Naples, FL Message-ID: Allied Search Partners is searching for a qualified Histotech to work in Naples, FL. To apply please send resume to Brannon@alliedsearchpartners.com Location: Naples, FL Job Title: Histotech Job Description: ? Receives and accesses all tissue, surgical specimens, foreign bodies, orthopedic devices, and other specimens removed at surgery and submitted from the operating room. ? Biopsies, aspiration specimens, and cytology specimens are also received and accessioned from the emergency room, nursing units, or outpatient procedures. ? Arranges for the submission of various tissues ? Maintains and relates supply requirements to the Assistant Laboratory Manager. ? Stores and disposes of specimens, paraffin blocks, and slides appropriately ? Assists the Pathologist in processing tissue for immediate frozen sections. ? Reports to an appropriate Section Supervisor or the Assistant Laboratory Manager, and is ultimately responsible to the Laboratory Manager/Pathologist. Requirements: Florida Clinical Lab or Histology License -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From afimbres <@t> uci.edu Thu Dec 29 14:43:41 2011 From: afimbres <@t> uci.edu (Fimbres, Amber) Date: Thu Dec 29 14:43:48 2011 Subject: [Histonet] CPT CODE 88106 and 88104 In-Reply-To: References: Message-ID: PROCEDURE CODES FOR NON-GYNECOLOGIC, NON-FINE-NEEDLE ASPIRATION SPECIMENS 88104: Direct smear 88108: Concentrated prep (cytospin or Saccomanno) 88112: Enriched or concentrated prep (e.g., ThinPrep(r), SurePath(r)) This information was taken from Cibas & Ducatman's Cytology: Diagnostic Principles and Clinical Correlates As for code 88106, I'm not sure what "simple filter method" they are referring to (Millipore?) but if you check out CAP's website, it seems they don't either. :) http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=cap_today%2Ffeature_stories%2F0107CPT.html&_state=maximized&_pageLabel=cntvwr Amber Amber M. Fimbres, MHA, CT(ASCP)CMHTLCM Cytotechnologist UC Irvine Medical Center -----Original Message----- From: Tunde Ajibade [mailto:tajibade@echd.org] Sent: Wednesday, December 28, 2011 10:47 AM To: histonet@lists.utsouthwestern.edu; Tunde Ajibade Subject: [Histonet] CPT CODE 88106 and 88104 Hello everyone, I need more information about the following CPT: CPT 88106-Cytopathology, fluids, washing or brushings, except cervical or vaginal: SIMPLE FILTER METHOD with interpretation CPT 88104 -Cytopathology, fluids, washing or brushings, except cervical or vaginal: smears with interpretation. My question is that, what is the SIMPLE FILTER METHOD means for CPT 88106? Does it means making of thin prep from the specimen? Which one is more appropriate to use? I need help. Thanks Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From madeleinehuey <@t> gmail.com Fri Dec 30 00:31:20 2011 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Fri Dec 30 00:31:27 2011 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 32 In-Reply-To: <4efcaacc.1e88650a.4310.ffff87a1SMTPIN_ADDED@mx.google.com> References: <4efcaacc.1e88650a.4310.ffff87a1SMTPIN_ADDED@mx.google.com> Message-ID: Gui, I don't see any problem with the protocol. Here's what I would do before waisting my time on the ICC staining. I check the viability of the cells before staining, because many thing can go wrong with the primary culture (my guest it's not cell line, am I right?). - To do this, at the end of culturing, I rinse off the medium with pre-chilled PBS without any ionic detergent (ie. Triton, Tween 20, & etc). - Add DAPI/PBS (your working concentration is fine) for 5 min @ RT. - Wash off excess DAPI with PBS x3. - Take the cells & check under the fluorescent microscope if the NUCLEI are in good condition (with proper filter). They should be the same morphology as your 1st day culture after they adherent. ** If they look the same as your ICC, then that mean it's not the protocol problem. It's your tissue culture problem. Primary rodent brain culture are Not easy to culture. Please let me know if my suggestion are helping or not. Good Luck! Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor-Pathology (IPOX & Histology) El Camino Hospitial madeleine_h@elcaminohospital.org On Thu, Dec 29, 2011 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. pap supply processing (Gale Limron) > ? 2. CPT CODE 88106 and 88104 (Tunde Ajibade) > ? 3. RE: Histonet Digest, Vol 97, Issue 31 (Goodwin, Diana) > ? 4. Shatter effect on specimens (Jimmy Lofton) > ? 5. Histologist needed UAMS Little Rock AR (Marcum, Pamela A) > ? 6. Saturday Coverage Orange County California (Paula Lucas) > ? 7. RE: Shatter effect on specimens (joelle weaver) > ? 8. C4d immunofluorescent positive controls (Martha Ward-Pathology) > ? 9. RE: C4d immunofluorescent positive controls (Tunde Ajibade) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 28 Dec 2011 13:06:13 -0500 > From: Gale Limron > Subject: [Histonet] pap supply processing > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > Happy Holidays! > Can anyone tell me what their current per-test pricing is for ThinPrep pap supplies? I'm putting together a study to present numbers to possibly bring our Gyn Cytology back in-house. > Thank you, > Gale > > Gale Limron CT,HT (ASCP) > Histology Supervisor > Union Hospital > 659 Boulevard > Dover, Ohio 44622 > 330-343-3311 ext 2562 > > > > This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. > > ------------------------------ > > Message: 2 > Date: Wed, 28 Dec 2011 12:46:50 -0600 > From: Tunde Ajibade > Subject: [Histonet] CPT CODE 88106 and 88104 > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ?, ? ?Tunde Ajibade > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > Hello everyone, > I need more information about the following CPT: > CPT 88106-Cytopathology, fluids, washing or brushings, except cervical or vaginal: SIMPLE FILTER METHOD with interpretation > CPT 88104 -Cytopathology, fluids, washing or brushings, except cervical or vaginal: smears with interpretation. > My question is that, what is the SIMPLE FILTER METHOD means for CPT 88106? Does it means making of thin prep from the specimen? Which one is more appropriate to use? I need help. Thanks > > ?Tunde Ajibade BS, HTL(ASCP)QIHC > > > > > CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. ?If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. > > > ------------------------------ > > Message: 3 > Date: Wed, 28 Dec 2011 13:53:11 -0500 > From: "Goodwin, Diana" > Subject: [Histonet] RE: Histonet Digest, Vol 97, Issue 31 > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ?<09411E0112A96A459D8D5FBDAB9C15C72480DAE383@HAMEXMBA.rwjham.local> > Content-Type: text/plain; charset="us-ascii" > > RE: standard for sm bx microtomy (Sharon Allen) > > Hi, Sharon. > > We are cutting 2 H&E levels per block, with 1 extra slide and 1 special stain slide in between - the special being dependent on the type of tissue-HP Giemsa for upper GI, PAS/AB for esophagus, Mucin for lung, etc. > > Hope this helps. > > Diana G. Goodwin, BS, HT(ASCP)QIHC > Department of Pathology > Robert Wood Johnson University Hospital at Hamilton > One Hamilton Health Place > Hamilton, NJ ?08690 > Ph: ?609.631.6996 > Email: ?dgoodwin@rwjuhh.edu > ?-----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu > Sent: Wednesday, December 28, 2011 1:04 PM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 97, Issue 31 > > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Re: Histonet Digest, Vol 97, Issue 30 (Thomas "Tom" Ehlers) > ? 2. Re: immuno stainers (Blazek, Linda) > ? 3. good protocol for ICC in neurons (Guillermo Palchik) > ? 4. Re: immuno stainers (Karen Lahti) > ? 5. gel pad for microtome vibration (Nancy Schmitt) > ? 6. FW: Quality Modules (Sharon Allen) > ? 7. FW: standard for sm bx microtomy (Sharon Allen) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 27 Dec 2011 14:56:54 -0500 > From: "Thomas \"Tom\" Ehlers" > Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 30 > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=windows-1252 > > Hi Cindy, > > What's the name of the new Dako stainer that you demo'd? > > On Tue, Dec 27, 2011 at 1:00 PM, > wrote: > >> Send Histonet mailing list submissions to >> ? ? ? ?histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> ? ? ? ?histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> ? ? ? ?histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> ? 1. RE: HSV1 and HSV2 (Hoekert, W.E.J.) >> ? 2. RELIA HOT Histology Job Alert. 12/27/2011 Great ? Opportunity >> ? ? ?for a histology tech in Virginia. (Pam Barker) >> ? 3. immuno stainers (Cynthia Pyse) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Tue, 27 Dec 2011 10:38:20 +0100 >> From: "Hoekert, W.E.J." >> Subject: RE: [Histonet] HSV1 and HSV2 >> To: "Evans, Andria B" , >> ? ? ? ? >> Message-ID: <1190CB05C44B13409483514729C2FC3601F84207@PAIT42.olvg.nl> >> Content-Type: text/plain; ? ? ? charset="iso-8859-1" >> >> For the immunologic pAB cocktail we are using Protease 1 (4 min) and ab >> incubation of 32 min. The only problem is that mast cells are also >> staining, it is a side effect of the protease 1. >> >> (AB concentration 1:400) >> >> Willem Hoekert >> OLVG Amsterdam >> The Netherlands >> >> >> >> >> ________________________________ >> >> Van: histonet-bounces@lists.utsouthwestern.edu namens Evans, Andria B >> Verzonden: vr 23-12-2011 18:48 >> Aan: histonet@lists.utsouthwestern.edu >> Onderwerp: [Histonet] HSV1 and HSV2 >> >> >> >> I am currently having issues with our HSV1 and HSV2 staining tissue >> components that it shouldn't be/background. ?We are running Ventana >> Benchmark XTs and Ultras. ?Using Dako's polyclonal rabbit concentrate. >> ?Does anyone out there in histoland have a concentration with a protocol >> that works and looks clean? >> >> Thank you for your help! >> >> Andria B Evans HTL(ASCP)CM >> This email was sent securely from the LGHealth Email Service >> >> Confidentiality Notice: >> This e-mail message, including any attachments, is for the sole use >> of intended recipient(s) and may contain confidential and >> privileged information. >> Any unauthorized review, use, disclosure or distribution is >> prohibited. >> If you are not the intended recipient, please contact the sender by >> reply e-mail and destroy all copies of the original message. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> Disclaimer: >> >> Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). >> Verstrekking aan en gebruik door anderen dan geadresseerden is niet >> toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de >> verzender hiervan op de hoogte te stellen en het bericht te verwijderen. >> In verband met electronische verzending kunnen aan dit e-mail bericht geen >> rechten worden ontleend. >> >> >> >> >> ------------------------------ >> >> Message: 2 >> Date: Tue, 27 Dec 2011 11:26:52 -0500 >> From: "Pam Barker" >> Subject: [Histonet] RELIA HOT Histology Job Alert. 12/27/2011 Great >> ? ? ? ?Opportunity for a histology tech in Virginia. >> To: "'Histonet'" >> Message-ID: <2AD338F5D60F42969C3852F762D09410@ownerf1abaad51> >> Content-Type: text/plain; ? ? ? charset="iso-8859-1" >> >> Hi Histonetters, >> Seasons Greetings!! >> I hope everyone enjoyed a wonderful holiday weekend. ?I have a brand new >> histology job that I am pretty excited about and I wanted to take a >> minute and share the info with you: >> >> RELIA Solutions the nation?s only recruiting firm dedicated to the >> nationwide permanent placement of histology professionals is assisting a >> leading lab in Roanoke, VA in their search for a histology tech. ?ASCP >> HT/HTL and 3 years experience preferred. ?IHC is a plus. ?This is a day >> shift (early morning) M-F full time permanent position. ?My client >> offers a great salary and excellent benefits. ?For more information >> please contact Pam Barker at ? >> relia1@earthlink.net or toll free at 866-607-3542 >> >> If you or anyone you know might be interested in hearing more about this >> opportunity please contact me. ?I can be reached at >> relia1@earthlink.net or toll free at >> 866-607-3542. >> >> Thanks-Pam >> >> >> Thank You! >> >> >> >> Pam Barker >> President >> RELIA >> Specialists in Allied Healthcare Recruiting >> 5703 Red Bug Lake Road #330 >> Winter Springs, FL 32708-4969 >> Phone: (407)657-2027 >> Cell: ? ? (407)353-5070 >> FAX: ? ? (407)678-2788 >> E-mail: ? relia1@earthlink.net >> ? www.facebook.comPamBarkerRELIA >> ? >> www.linkedin.com/reliasolutions >> ? www.myspace.com/pamatrelia >> ? www.twitter.com/pamatrelia >> >> >> >> ------------------------------ >> >> Message: 3 >> Date: Tue, 27 Dec 2011 11:49:50 -0500 >> From: "Cynthia Pyse" >> Subject: [Histonet] immuno stainers >> To: >> Message-ID: <003e01ccc4b7$8cb51a40$a61f4ec0$@com> >> Content-Type: text/plain; ? ? ? charset="us-ascii" >> >> Hello Histonetters >> >> Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how >> times flies when you have time off. Now it's back to the old laboratory >> grind. >> >> We are in the market for a new immuno stainer. I currently use a Dako Link, >> which I am very pleased with. I have demoed the new Dako with great >> results, >> unfortunately we need 2 stainers and the pricing maybe a little out of our >> range. >> >> Is anyone using the Biocare Intellipath? What are the pro's and con's? How >> is their service? I currently use their detection system, Mach 4, but the >> majority of my antibodies are purchased from Dako. Any input on the daily >> use of the Intellipath from current users would be appreciated. >> >> Thanks in advance for the input >> >> Cindy >> >> >> >> Cindy Pyse, CLT, HT (ASCP) >> >> Laboratory Manager >> >> X-Cell Laboratories >> >> e-mail cpyse@x-celllab.com >> >> >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 97, Issue 30 >> **************************************** >> > > > ------------------------------ > > Message: 2 > Date: Tue, 27 Dec 2011 14:58:46 -0500 > From: "Blazek, Linda" > Subject: Re: [Histonet] immuno stainers > To: Cynthia Pyse > Cc: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > I have the Intellipath from BioCare. ?I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company ?to work with in both sales or service. If you want to contact me directly feel. > Linda Blazek > GI Pathology > 937 396-2623 > > Sent from my iPhone > > On Dec 27, 2011, at 11:51 AM, "Cynthia Pyse" wrote: > >> Hello Histonetters >> >> Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how >> times flies when you have time off. Now it's back to the old laboratory >> grind. >> >> We are in the market for a new immuno stainer. I currently use a Dako Link, >> which I am very pleased with. I have demoed the new Dako with great results, >> unfortunately we need 2 stainers and the pricing maybe a little out of our >> range. >> >> Is anyone using the Biocare Intellipath? What are the pro's and con's? How >> is their service? I currently use their detection system, Mach 4, but the >> majority of my antibodies are purchased from Dako. Any input on the daily >> use of the Intellipath from current users would be appreciated. >> >> Thanks in advance for the input >> >> Cindy >> >> >> >> Cindy Pyse, CLT, HT (ASCP) >> >> Laboratory Manager >> >> X-Cell Laboratories >> >> e-mail cpyse@x-celllab.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Tue, 27 Dec 2011 16:32:06 -0500 > From: Guillermo Palchik > Subject: [Histonet] good protocol for ICC in neurons > To: histonet@lists.utsouthwestern.edu > Message-ID: <80DAFD47-401A-4FAF-AB05-32EC6AA81364@georgetown.edu> > Content-Type: text/plain; ? ? ? charset=us-ascii > > Dear histonetters, > > I am trying to do some ICC on nuclei of rat primary neurons that have been grown for 2 weeks on coverslips (seeded at 75,000 cells/ml). So far I have followed the protocol that a (long since gone) student in the lab used, but I have been unsuccessful. My nuclei end up looking like raisins.... > The protocol that the student used is unlike anything I have seen: > Wash with cold PBS 2X 5 min > > Rinse briefly with cold methanol > > Fix in methanol at -20C for 20 minutes > > Wash in cold PBS 2 X 10 min > > Block in 5% NGS / 0.05% Triton-X for 6 hours at 4C > > Add Primary antibodies diluted in Blocking Solution overnight @ 4C > > Wash in cold Wash Solution (2% NGS/0.05% Triton-X) 3 X 10 min > > Add Secondary Antibodies diluted in Blocking Solution for 1.5 hours at RT ?in dark > > Wash in Wash Solution 3 X 20 min in dark > > > Wash in PBS 3 X 10 min in dark > > Add DAPI (1:10,000 in PBS) for 5 minutes in dark > > Wash in ddH2O 3 X 10 min in dark > > Mount with Fluoro-Gel with Tris > > Store @ 4C in dark until dry > > > > First, after a methanol fixation of 20 minutes at -20C the protocol calls for blocking and permeabilizing the cells for 6 hours! > > Second, it keeps washing with this washing solution that contains triton. > > As I said, I have followed this protocol to the T and I keep getting horrible looking, shriveled cells and nuclei... > The question I have is, does the protocol look okay and maybe it is me, or should I change specific parameters of it? > > Does anybody have a good working protocol that could share with me? It would be greatly appreciated... > Thanks > Gil > > -- > Guillermo Palchik > Ph.D. Candidate - Interdisciplinary Program in Neuroscience > Georgetown University Medical Center > Research Building Room W 217 > 3970 Reservoir Rd. NW, Washington, DC 20007 > Lab: 202-687-7825 > > > > ------------------------------ > > Message: 4 > Date: Tue, 27 Dec 2011 19:34:36 -0700 > From: Karen Lahti > Subject: Re: [Histonet] immuno stainers > To: "Blazek, Linda" > Cc: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > Content-Type: text/plain; ? ? ? charset=us-ascii > > We have the IntelliPath as well. ?I have the same sentiments as Linda regarding all aspects of Biocare and the instrument. ?We use their antibodies and detection. ?The company as a whole is excellent to deal with and very customer oriented. > > Thanks, Karen Lahti > Arizona Digestive Health > Phoenix, AZ > > > On Dec 27, 2011, at 12:58 PM, "Blazek, Linda" wrote: > >> I have the Intellipath from BioCare. ?I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company ?to work with in both sales or service. If you want to contact me directly feel. >> Linda Blazek >> GI Pathology >> 937 396-2623 >> >> Sent from my iPhone >> >> On Dec 27, 2011, at 11:51 AM, "Cynthia Pyse" wrote: >> >>> Hello Histonetters >>> >>> Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how >>> times flies when you have time off. Now it's back to the old laboratory >>> grind. >>> >>> We are in the market for a new immuno stainer. I currently use a Dako Link, >>> which I am very pleased with. I have demoed the new Dako with great results, >>> unfortunately we need 2 stainers and the pricing maybe a little out of our >>> range. >>> >>> Is anyone using the Biocare Intellipath? What are the pro's and con's? How >>> is their service? I currently use their detection system, Mach 4, but the >>> majority of my antibodies are purchased from Dako. Any input on the daily >>> use of the Intellipath from current users would be appreciated. >>> >>> Thanks in advance for the input >>> >>> Cindy >>> >>> >>> >>> Cindy Pyse, CLT, HT (ASCP) >>> >>> Laboratory Manager >>> >>> X-Cell Laboratories >>> >>> e-mail cpyse@x-celllab.com >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Wed, 28 Dec 2011 14:21:44 +0000 > From: Nancy Schmitt > Subject: [Histonet] gel pad for microtome vibration > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<906B4DA90ED1DB4DB6C7E94D7CEE6C367BC785@PEITHA.wad.pa-ucl.com> > Content-Type: text/plain; charset="us-ascii" > > Good Morning- > I am looking for some type of gel pad to place under the microtome (or perhaps under the waterbath...) to help with vibration - the water in the waterbath is jumping all over the place. ?Both instruments are new and the waterbath is much lighter in weight than the old ones. > Thank you for your help with this! > Nancy > Dubuque, IA > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > > ------------------------------ > > Message: 6 > Date: Wed, 28 Dec 2011 08:34:07 -0600 > From: "Sharon Allen" > Subject: [Histonet] FW: Quality Modules > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > I am looking for information on Quality Modules that can be added into > an existing IT system and system with great Quality Modules built into > their IT platform. > > Thanks > > > > Sharon Allen > > Senior Medical Technologist > > Neuropathology Lab-MS435U > > Health Sciences Centre > > 820 Sherbrook Street > > Winnipeg,MB, CA > > R3A 1R9 > > e-mail: sallen@dsmanitoba.ca > > > > -------------- next part -------------- > This email and/or any documents in this transmission is intended for the > addressee(s) only and may contain legally privileged or confidential information. ?Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. ?If you receive this transmission in error, please notify the sender immediately and return the original. > > Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. > > ------------------------------ > > Message: 7 > Date: Wed, 28 Dec 2011 08:40:11 -0600 > From: "Sharon Allen" > Subject: [Histonet] FW: standard for sm bx microtomy > To: > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > Hi, > > Is there is a standard used for small biopsy Microtomy? ?I am wondering > how other sites cut their biopsies (ie the number of sections per slide > and the amount of roughing in between sections)? ?I am also curious > about the number of slides per biopsy. > > Thanks for your help, > > > > Sharon Allen > > Senior Medical Technologist > > Neuropathology Lab-MS435U > > Health Sciences Centre > > 820 Sherbrook Street > > Winnipeg,MB, CA > > R3A 1R9 > > e-mail: sallen@dsmanitoba.ca > > > > -------------- next part -------------- > This email and/or any documents in this transmission is intended for the > addressee(s) only and may contain legally privileged or confidential information. ?Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. ?If you receive this transmission in error, please notify the sender immediately and return the original. > > Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 31 > **************************************** > > > > ------------------------------ > > Message: 4 > Date: Thu, 29 Dec 2011 09:35:10 -0500 > From: "Jimmy Lofton" > Subject: [Histonet] Shatter effect on specimens > To: Histonet@lists.utsouthwestern.edu > Message-ID: <4EFC344E020000560000507C@nodcdmg2.no.trinity-health.org> > Content-Type: text/plain; charset=us-ascii > > Does anyone in histoland have corrected problems with shattered biopies, EM ect? > > Thanks for your help. > > Jimmy > > > Jimmy Lofton, M.S., HT,CT(ASCP) > Manager Histology Laboratory > Holy Cross Hospital > 1500 Forest Glen Road > Silver Spring, MD ?20910-1484 > 301-754-7353?(Phone) > 301-754-8563?(Fax) > loftonjt@holycrosshealth.org > > > Trinity Health MailGate made the following annotations > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. > --------------------------------------------------------------------- > > > > ------------------------------ > > Message: 5 > Date: Thu, 29 Dec 2011 14:53:31 +0000 > From: "Marcum, Pamela A" > Subject: [Histonet] Histologist needed UAMS Little Rock AR > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<41D3A1AF6FEF0643BDC89E0516A6EA3220240815@Mail2Node2.ad.uams.edu> > Content-Type: text/plain; charset="us-ascii" > > Please no recruiters reply to this. ?We are a state University and we are not allowed to use your services. ?I am sorry however; this saves us both time. > > We currently have an opening for a registered HT or HTL at the University of Arkansas for Medical Sciences in Little Rock AR. ?It is a full time position with full benefits, vacation, sick time and 12 paid holidays per year. ?We are fast growing Histology Laboratory with new equipment and procedures coming for more experience with growth potential. ?The position is listed under UAMS Jobs as # 50006966. ?If you have any question you may call me at the number below. ?I will be happy to discuss the position and future plans with you. > > Best Regards, > > Pamela A Marcum > AP Supervisor Histology > Slot 502 > 4301 W Markham Street > Little Rock AR 72205 > Office: 501-686-7554 > Fax: 501-686-7151 > > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. ?Any unauthorized review, > use, disclosure or distribution is prohibited. ?If you are not the > intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message.. > > > ------------------------------ > > Message: 6 > Date: Thu, 29 Dec 2011 07:21:06 -0800 > From: "Paula Lucas" > Subject: [Histonet] Saturday Coverage Orange County California > To: > Message-ID: <300217F9262C41D2A82C5951202C8995@biopath.local> > Content-Type: text/plain; ? ? ? charset="us-ascii" > > We are in need of a per diem histotech who can work on Saturday mornings. > We are located in Fountain Valley, California. Please send me an email if > interested or fax resume to: > > 714 755-2984 > > Thank you, > > Paula Lucas > > > > ------------------------------ > > Message: 7 > Date: Thu, 29 Dec 2011 16:38:33 +0000 > From: joelle weaver > Subject: RE: [Histonet] Shatter effect on specimens > To: Jimmy Lofton > Cc: Histonet , > ? ? ? ?histonet-bounces@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Chatter or shatter? Chatter to me is a microtomy problem more so, shatter I would look more to processing and handling... but people use those descriptions to mean both sometimes, but here are my thoughts #1 tissue type considerations - GI Bx and liver Bx especially- I have seen this alot when they get processed all together with other tissues( in a "do everything type of program"). #2- over dehydration, too prolonged or not enough gradation from dilute to absolute ETOH ?for certian tissue types( biopsies here) in the tissue processing program espeically those types in #1. #3 sometimes also more problems when rapid processing used with MW or when they had "rapid" processors with acetone ?espeically( gone now I think, the original Sakura), but the program just needs to be optimized if that is the case. #4 poor microtomy technique, and/or loose blade holder, block holder (due to rusted, worn out screws, or springs ) especially the little ones in the blade holder, or just not being clamped properly, or the angle can be off- this is the "chatter". Sometimes something is loose or worn, but some people clamp everything super-tight, and this is sometimes trouble too, or can't correct their angles. #5- I guess you would need to figure out what the source is intially to correct. But all could possibly be corrected with program changes to processing, microtome PM, training of microtomist to recognize the artifact on waterbath before it gets to the slide, and get more comfortable/confident with their microtome. At the bench ?I can correct the less than ideal processing sometimes when already "crunchy" by somewhat prolonged soaking in icy water before sectioning, and trying to section a little slower than I normally would. Can't help much with EM, not my experience, but I would guess some of the same issues, and/or vibration on the ultramicrotome?Joelle > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > ?> Date: Thu, 29 Dec 2011 09:35:10 -0500 >> From: loftonjt@holycrosshealth.org >> To: Histonet@lists.utsouthwestern.edu >> CC: >> Subject: [Histonet] Shatter effect on specimens >> >> Does anyone in histoland have corrected problems with shattered biopies, EM ect? >> >> Thanks for your help. >> >> Jimmy >> >> >> Jimmy Lofton, M.S., HT,CT(ASCP) >> Manager Histology Laboratory >> Holy Cross Hospital >> 1500 Forest Glen Road >> Silver Spring, MD ?20910-1484 >> 301-754-7353 (Phone) >> 301-754-8563 (Fax) >> loftonjt@holycrosshealth.org >> >> >> Trinity Health MailGate made the following annotations >> --------------------------------------------------------------------- >> CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. >> --------------------------------------------------------------------- >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 8 > Date: Thu, 29 Dec 2011 17:03:07 +0000 > From: Martha Ward-Pathology > Subject: [Histonet] C4d immunofluorescent positive controls > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. ? Thanks in advance for any and all responses. > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center > Winston-Salem, NC 27157 > 336-716-2104 > > > > ------------------------------ > > Message: 9 > Date: Thu, 29 Dec 2011 11:53:11 -0600 > From: Tunde Ajibade > Subject: [Histonet] RE: C4d immunofluorescent positive controls > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > > > Do you do renal transplant? If yes, look for renal biopsies submitted for immunofluorescence studies, Let the Pathologists confirm that the biopsy is acute cellular rejection from renal transplant, because acute cellular rejection from kidney transplant is known to be positive for c4d. After the studies, cut some extra frozen sections from this biopsy and fix in acetone for 20 mins, and cover the slide with parafilm to avoid cracking of slide and store in the freezer. They are good for some months. > > ?Tunde Ajibade BS, HTL(ASCP)QIHC > ?Histology Supervisor > ?Medical Center Hospital > ?Odessa,TX > ?Tel:432-640-2348 > ?Fax:432-640-2303 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology > Sent: Thursday, December 29, 2011 11:03 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] C4d immunofluorescent positive controls > > We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. ? Thanks in advance for any and all responses. > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center > Winston-Salem, NC 27157 > 336-716-2104 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. ?If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 32 > **************************************** From sfonner <@t> labpath.com Fri Dec 30 05:57:04 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Fri Dec 30 06:02:44 2011 Subject: [Histonet] MiTF Message-ID: <000001ccc6ea$25bbbfd0$71333f70$@com> Happy Holidays Histoland, Does anyone out there have a good working protocol on the Ventana Ultra for MiTF. I have tried several but the docs still aren't happy with it. Could you share any info you may have regarding vendor, system, protocol, etc.? Much thanks! Sheila, HT (ASCP) KDL Pathology Knoxville, TN From dtaylor <@t> mcpathology.com Fri Dec 30 07:41:42 2011 From: dtaylor <@t> mcpathology.com (Debbie Taylor) Date: Fri Dec 30 07:44:09 2011 Subject: [Histonet] Histotech position Message-ID: <107F5E0D3BEF5843BF653A6F629694F624A97C@mcpexchange.mcp.local> We are currently recruiting a histotech for our small independent pathology lab located in South Carolina. We offer a competitive salary, health insurance, sick leave, paid vacation, holidays, 401K/profit sharing. The hours are 5am-1pm. The job would include but not limited to embedding, cutting, staining, and knowledge of immunohistochemistry. Please call or email me if you are interested. Thanks! Deborah Taylor, MS, CT(ASCP) Customer Relations/Lab Manager Marlboro Chesterfield Pathology, PC 672 Hwy 9 West Bennettsville, SC 29512 Phone: 843-479-2402 Fax: 843-479-6609 Note: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anone else is unauthorized. If you are not the intended recipient, any disclosure copying or distribution of the message or any action or omission taken by you in reliance on it, is prohibitied and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. From gp62 <@t> georgetown.edu Fri Dec 30 12:00:05 2011 From: gp62 <@t> georgetown.edu (Guillermo Palchik) Date: Fri Dec 30 12:00:12 2011 Subject: [Histonet] Coverslip Sealant Message-ID: <3894CE1F-29DF-48EB-BE75-05EC97F3F9D2@georgetown.edu> Hi there, Does anybody know of a good alternative to nail polish for sealing round coverslips (or any shape, for that matter!). Ideally I am looking for a solution that is both an aqueous mount media and sealant, if such a beast exists... Thanks! Gil PS: Happy New Year! -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 From SJMccabe <@t> drmc.org Fri Dec 30 12:02:55 2011 From: SJMccabe <@t> drmc.org (McCabe, Sara J.) Date: Fri Dec 30 12:03:01 2011 Subject: [Histonet] IHC on Bones Message-ID: <02AE2390303AAB43A823930EAD6B63AE26197DEE@EX07.drmc.org> Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. From liz <@t> premierlab.com Fri Dec 30 12:04:48 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Dec 30 12:07:08 2011 Subject: [Histonet] RE: IHC on Bones In-Reply-To: <02AE2390303AAB43A823930EAD6B63AE26197DEE@EX07.drmc.org> References: <02AE2390303AAB43A823930EAD6B63AE26197DEE@EX07.drmc.org> Message-ID: <14E2C6176416974295479C64A11CB9AE011380AD9D10@SBS2K8.premierlab.local> Sara If you can you want to stick with an enzyme digestion such as proteinase K or another enzyme. If the antibody requires HIER and will not work with enzyme digestion then modify your retreival time and temp, we use 70C for 2 hours and find that this works for most antibodies. Some antibodies that require HIER may not work with the longer/lower temp methods. Good Luck Liz ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara J. [SJMccabe@drmc.org] Sent: Friday, December 30, 2011 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Bones Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Fri Dec 30 12:18:20 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Dec 30 12:18:31 2011 Subject: [Histonet] IHC on Bones In-Reply-To: <02AE2390303AAB43A823930EAD6B63AE26197DEE@EX07.drmc.org> References: <02AE2390303AAB43A823930EAD6B63AE26197DEE@EX07.drmc.org> Message-ID: <4EFDBA1C.2B7F.00C9.1@geisinger.edu> pray. >>> "McCabe, Sara J." 12/30/2011 1:02 PM >>> Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Dec 30 12:26:24 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Dec 30 12:27:27 2011 Subject: [Histonet] RE: IHC on Bones In-Reply-To: <02AE2390303AAB43A823930EAD6B63AE26197DEE@EX07.drmc.org> References: <02AE2390303AAB43A823930EAD6B63AE26197DEE@EX07.drmc.org> Message-ID: I have place the deparaffinized slides into 10% neutral buffered formalin for about 30 minutes before the heat retrieval. Somehow this seems to work You can also lower the temperature of your retrieval and lengthen the time. Good Luck. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara J. [SJMccabe@drmc.org] Sent: Friday, December 30, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Bones Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Fri Dec 30 12:33:17 2011 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Dec 30 12:33:22 2011 Subject: [Histonet] NCCI policy update Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int> Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From mward <@t> wakehealth.edu Fri Dec 30 13:12:36 2011 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Fri Dec 30 13:12:47 2011 Subject: [Histonet] RE: NCCI policy update In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int> Message-ID: Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Montina.VanMeter <@t> pbrc.edu Fri Dec 30 13:23:06 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri Dec 30 13:23:12 2011 Subject: [Histonet] Coverslip Sealant In-Reply-To: <3894CE1F-29DF-48EB-BE75-05EC97F3F9D2@georgetown.edu> References: <3894CE1F-29DF-48EB-BE75-05EC97F3F9D2@georgetown.edu> Message-ID: ProLong Gold (Invitrogen) Montina J. Van Meter, HT (ASCP) Lab Manager Dept. of Autonomic Neuroscience Pennington Biomedical Research Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo Palchik Sent: Friday, December 30, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslip Sealant Hi there, Does anybody know of a good alternative to nail polish for sealing round coverslips (or any shape, for that matter!). Ideally I am looking for a solution that is both an aqueous mount media and sealant, if such a beast exists... Thanks! Gil PS: Happy New Year! -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Fri Dec 30 14:06:45 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Dec 30 14:05:24 2011 Subject: [Histonet] RE: NCCI policy update In-Reply-To: References: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int> Message-ID: <5A33C952BB67F4468AF1F36D739212BC07382E@JERRY.Gia.com> Where can I get the article to show my billing dept/pathologists? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Friday, December 30, 2011 1:13 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: NCCI policy update Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Dec 30 15:16:55 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Dec 30 15:18:22 2011 Subject: [Histonet] NCCI policy update In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939537FC6D@chimsx08.CHI.catholichealth.net> I would expect the trend to go toward specimen and not block, perhaps even case and not specimen. It comes down to health care cost reduction and health-care reform which are turning out to be the same thing. I don' care who's holding the wheel, so long as gov't is doing the driving, we can expect this sort of thing. - Have a great new year - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 12:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From histotech <@t> imagesbyhopper.com Fri Dec 30 15:21:52 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Dec 30 15:21:56 2011 Subject: [Histonet] RE: NCCI policy update In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC07382E@JERRY.Gia.com> References: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int> <5A33C952BB67F4468AF1F36D739212BC07382E@JERRY.Gia.com> Message-ID: <004301ccc739$0f68ee20$2e3aca60$@imagesbyhopper.com> Wow. What made them change their minds? There are many times when we stain different blocks of a single specimen and only one lights up. I, too, would be interested in an official document stating the change. Thanks! Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, December 30, 2011 3:07 PM To: Martha Ward-Pathology; Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: NCCI policy update Where can I get the article to show my billing dept/pathologists? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Friday, December 30, 2011 1:13 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: NCCI policy update Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: 12/30/11 From JEllin <@t> yumaregional.org Fri Dec 30 16:18:17 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Dec 30 16:18:24 2011 Subject: [Histonet] RE: NCCI policy update In-Reply-To: <004301ccc739$0f68ee20$2e3aca60$@imagesbyhopper.com> References: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int> <5A33C952BB67F4468AF1F36D739212BC07382E@JERRY.Gia.com>, <004301ccc739$0f68ee20$2e3aca60$@imagesbyhopper.com> Message-ID: Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow. What made them change their minds? There are many times when we stain > different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for each > block regarding IHC billing, only one unit of billing for each part type no > matter how many blocks are stained? Also IHC "cocktail" stains, such as > PIN4 must now be billed as one unit even though multiple antibodies are > reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC codes > 88342, 88360, and 88361. > > Happy New Year to everyone out there. May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From histotalk <@t> yahoo.com Sat Dec 31 06:56:45 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Sat Dec 31 06:56:48 2011 Subject: [Histonet] Three Announcements! Message-ID: <1325336205.8393.YahooMailNeo@web120606.mail.ne1.yahoo.com> First - HAPPY NEW YEAR! I am very happy to announce that as of tomorrow, January 1st, I begin my new position?as Florida Histology Consultant for?IOP In-Office Pathology www.IOPathology.com. Thanks Bernie Ness and Joe Plandowski! ? Second - HistoTALK resumes on Januaary 8th, next Sunday, after a two month hiatus.Whew! Needed the break. Our guest will be a very exciting, informative and knowledgeable speaker / educator Peggy Wenk! The interview was done at the 2011 NSH Symposium/Convention in Cincinnati. Peggy's a fun guest! Future guests will include Lamar Jones, Pam Barker, Damien Laudier, Vince Della Speranza and YOU! ? Third - HistoTALK will?have its "mobile" studio at the?2012 Region III meeting, hosted by our friends of the Georgia Society for Histotechnology. Thank you President Mike Ayers, Vice Pres. Wanda Simons and one of the founders of the GSH, Secretary Shirely Powell for the invitation! The Region III meeting will be held at the Mountain Creek Inn - Callaway Gardens in Georgia?April 13, 14 & 15. If you would like to be?a guest on HistoTALK?while at the Region III meeting, let Wanda Simons or I know and we'll get you scheduled. ? Happy Holidays!? ? Yours, Dave? From tmoore9k <@t> gmail.com Sat Dec 31 07:44:53 2011 From: tmoore9k <@t> gmail.com (Teresa Moore) Date: Sat Dec 31 07:45:01 2011 Subject: [Histonet] Cutting speed Message-ID: I graduated from a histology program in June/11 and just got a job a week ago. My speed on the microtome is not great. Everyone says it takes time but I feel my technique may be wrong. To make matters worse the only other histotech in the lab is going on vacation the third week of January and I will be alone!!!!! I don't have the overall flow of the lab down yet and have no idea how they expect me to handle the cutting all by myself. My biggest concern is my cutting speed right now. How long does it take (approx) to do 40 blocks an hour. Currently, I'm about half that! I'm panicking and I've only been on the job 8 days. Help!!!!!!!!!!! -- Teresa Moore From rjbuesa <@t> yahoo.com Sat Dec 31 08:55:05 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 31 08:55:10 2011 Subject: [Histonet] Cutting speed In-Reply-To: Message-ID: <1325343305.308.YahooMailClassic@web65710.mail.ac4.yahoo.com> You asked "how long does it take to (approx) to do 40 blocks an hour", well it takes an hour. If you meant "how long does it take to do 40 blocks", the answer is 1.7 hours, because the average cutting speed is 24 blocks per hour. Ren? J. --- On Sat, 12/31/11, Teresa Moore wrote: From: Teresa Moore Subject: [Histonet] Cutting speed To: histonet@lists.utsouthwestern.edu Date: Saturday, December 31, 2011, 8:44 AM I graduated from a histology program in June/11 and just got a job a week ago.? My speed on the microtome is not great.? Everyone says it takes time but I feel my technique may be wrong.? To make matters worse the only other histotech in the lab is going on vacation the third week of January and I will be alone!!!!! I don't have the overall flow of the lab down yet and have no idea how they expect me to handle the cutting all by myself.? My biggest concern is my cutting speed right now.? How long does it take (approx) to do 40 blocks an hour.? Currently, I'm about half that!? I'm panicking and I've only been on the job 8 days.? Help!!!!!!!!!!! -- Teresa Moore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail2 <@t> gmail.com Sat Dec 31 09:09:43 2011 From: renafail2 <@t> gmail.com (Rena Fail) Date: Sat Dec 31 09:09:47 2011 Subject: [Histonet] Cutting speed In-Reply-To: References: Message-ID: Teresa, How do your sections look? Good quality well stained sections are vital. Speed will come with experience. When it becomes second nature to know when a block is cold enough to get good sections without cracks from being too cold or when a bloody piece of tissue is moistened enough toget sections without swollen cells your speed will increase. You will have been working a month by the time the tech goes on vacation and will have the workflow down by then. Don't worry so much. no one will expect you to be as fast as a tech with years of experience. Good luck rena Fail On Sat, Dec 31, 2011 at 8:44 AM, Teresa Moore wrote: > I graduated from a histology program in June/11 and just got a job a week > ago. My speed on the microtome is not great. Everyone says it takes time > but I feel my technique may be wrong. To make matters worse the only other > histotech in the lab is going on vacation the third week of January and I > will be alone!!!!! I don't have the overall flow of the lab down yet and > have no idea how they expect me to handle the cutting all by myself. My > biggest concern is my cutting speed right now. How long does it take > (approx) to do 40 blocks an hour. Currently, I'm about half that! I'm > panicking and I've only been on the job 8 days. Help!!!!!!!!!!! > > -- > Teresa Moore > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From estellamireles <@t> gmail.com Sat Dec 31 09:13:53 2011 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Sat Dec 31 09:13:55 2011 Subject: [Histonet] Cutting speed In-Reply-To: References: Message-ID: Teresa please express your concerns to your supervisor or lab manager now ! A new person in a lab needs more time than one month to get used to the routine feel of a new place. Did they use a PRN during their search for the new tech. If so, have that person come in and help during the vacation break of the the other tech. Our utmost concern should be care of the patient and safety of the tech. I know from personal experience that both will be compromised when you have a situation like yours. You will be rushed and tissue samples can be lost in embedding or cut away during microtomy. You could cut yourself also. I am not even taking into account the other responsibilities of a general histology lab that will be compromised as well. The histo students in our area are encouraged to be able to trim and cut 30 blocks in one hour. This does not include the time to embed the tissue, nor the time to stain and sign out the case. Worst case scenario; the other tech will have to delay the scheduled vacation until you are thoroughly acclimated to the flow of the lab. My personal opinion is that the this situation is totally unfair to any new employee, seasoned or new graduate. I would never do this to a new tech myself. Stay in our field, be encouraged, it does get better Teresa. Have a Great and Prosperous New Year Stella From LRaff <@t> uropartners.com Sat Dec 31 09:56:57 2011 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Sat Dec 31 10:00:13 2011 Subject: [Histonet] RE: NCCI policy update References: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int><5A33C952BB67F4468AF1F36D739212BC07382E@JERRY.Gia.com>, <004301ccc739$0f68ee20$2e3aca60$@imagesbyhopper.com> Message-ID: Below is the quote from the new NCCI guidelines that deal with this issue. I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax: 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow. What made them change their minds? There are many times when we stain > different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for each > block regarding IHC billing, only one unit of billing for each part type no > matter how many blocks are stained? Also IHC "cocktail" stains, such as > PIN4 must now be billed as one unit even though multiple antibodies are > reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC codes > 88342, 88360, and 88361. > > Happy New Year to everyone out there. May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Sat Dec 31 11:13:17 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Dec 31 11:13:23 2011 Subject: [Histonet] RE: NCCI policy update In-Reply-To: References: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int><5A33C952BB67F4468AF1F36D739212BC07382E@JERRY.Gia.com>, <004301ccc739$0f68ee20$2e3aca60$@imagesbyhopper.com> Message-ID: <1325351597.41488.YahooMailNeo@web112313.mail.gq1.yahoo.com> The practice of billing for seperate blocks on one specimen such as A1, A2 and A3 I beleive has long been gone. At least in the organizations I have been with. What seems to be new is they are going after the new trend of using cocktail antibodies. I agree you would think that ASCP and CAP would be on top of this. While i might see the argument that?the technical component could be reduced in using these cocktails, I cant see how they think it will benefit anyone for not allowing the fee for each antibody read out on one slide from the Pathologist. I realize most places bill global fee's, so that's a issue that would need to be looked at how to deal with it. But it is a bit unfair when you consider sometimes you only have one tiny little specimen, or maybe even one slide to stain and you need multiple markers. You could even argue that the cost of using cocktails is more expensive for your technical component. If what they do is force labs to go back to using one slide per anibody, I dont feel thats a betterment in healthcare pratcice since sometimes tumor cells are limited to one or two slides per specimen. We've all seen it i'm sure. With all that said, with everything coming down the pipelines at us healthcare professionals, we need to buckle up. It's getting ugly out here.They used to want us (healthcare professionals)?to do a lot for a little bit, now its do everything with nothing.?There I said it. ? Kim Donadio ________________________________ From: Lester Raff MD To: Jesus Ellin ; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; "Webb, Dorothy L" Sent: Saturday, December 31, 2011 10:56 AM Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue.? I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax:? 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we stain > different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for each > block regarding IHC billing, only one unit of billing for each part type no > matter how many blocks are stained?? Also IHC "cocktail" stains, such as > PIN4 must now be billed as one unit even though multiple antibodies are > reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC codes > 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Sat Dec 31 11:18:21 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Dec 31 11:18:28 2011 Subject: [Histonet] Cutting speed In-Reply-To: References: Message-ID: <1325351901.53131.YahooMailNeo@web112319.mail.gq1.yahoo.com> My only advice to you Teresa is to take a deep breath, calm down and do the best you can. Dont take your eye off the specimen you are dealing with. It's someones life. You might hear people screaming about time, they need this, they need that. but You as a healthcare professional have the ONE most importnat task you really need to focus on, and thats making the best slide you can from each specimen you deal with. Focus on that, keep your chin up and know that you are doing the patients a service by being there doing good work while dealing with hard times. ? Best of wishes ? Kim D ________________________________ From: Teresa Moore To: histonet@lists.utsouthwestern.edu Sent: Saturday, December 31, 2011 8:44 AM Subject: [Histonet] Cutting speed I graduated from a histology program in June/11 and just got a job a week ago.? My speed on the microtome is not great.? Everyone says it takes time but I feel my technique may be wrong.? To make matters worse the only other histotech in the lab is going on vacation the third week of January and I will be alone!!!!! I don't have the overall flow of the lab down yet and have no idea how they expect me to handle the cutting all by myself.? My biggest concern is my cutting speed right now.? How long does it take (approx) to do 40 blocks an hour.? Currently, I'm about half that!? I'm panicking and I've only been on the job 8 days.? Help!!!!!!!!!!! -- Teresa Moore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Sat Dec 31 11:21:28 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sat Dec 31 11:21:42 2011 Subject: [Histonet] RE: NCCI policy update In-Reply-To: References: <65365F35C0F2EF4D846EC3CA73E49C43013480553B5B@HPEMX3.HealthPartners.int><5A33C952BB67F4468AF1F36D739212BC07382E@JERRY.Gia.com>, <004301ccc739$0f68ee20$2e3aca60$@imagesbyhopper.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F7B719@SMCMAIL01.somerset-healthcare.com> Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue. I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax: 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow. What made them change their minds? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there. May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From JWeems <@t> sjha.org Sat Dec 31 12:03:27 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sat Dec 31 12:03:32 2011 Subject: [Histonet] RE: NCCI policy update plus copper stain CPT change Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408454D132C@CHEXCMS10.one.ads.che.org> For the past two years we have been able to bill immunos and special stains per block. This will be a loss for for the big cases that need multiple blocks stained since the cost is per slide, not specimen. I don't see anything about the special stains tho - doesn't make sense. Global billing is technical and professional charges combined and would have include the separate blocks. NOTE: The CPT code has changed for the copper stain - 88318. It is now in the non-microbial group - 88313. Happy New Year Everyone!! j ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio [one_angel_secret@yahoo.com] Sent: Saturday, December 31, 2011 12:13 PM To: Lester Raff MD; Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update The practice of billing for seperate blocks on one specimen such as A1, A2 and A3 I beleive has long been gone. At least in the organizations I have been with. What seems to be new is they are going after the new trend of using cocktail antibodies. I agree you would think that ASCP and CAP would be on top of this. While i might see the argument that the technical component could be reduced in using these cocktails, I cant see how they think it will benefit anyone for not allowing the fee for each antibody read out on one slide from the Pathologist. I realize most places bill global fee's, so that's a issue that would need to be looked at how to deal with it. But it is a bit unfair when you consider sometimes you only have one tiny little specimen, or maybe even one slide to stain and you need multiple markers. You could even argue that the cost of using cocktails is more expensive for your technical component. If what they do is force labs to go back to using one slide per anibody, I dont feel thats a betterment in healthcare pratcice since sometimes tumor cells are limited to one or two slides per specimen. We've all seen it i'm sure. With all that said, with everything coming down the pipelines at us healthcare professionals, we need to buckle up. It's getting ugly out here.They used to want us (healthcare professionals) to do a lot for a little bit, now its do everything with nothing. There I said it. Kim Donadio ________________________________ From: Lester Raff MD To: Jesus Ellin ; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; "Webb, Dorothy L" Sent: Saturday, December 31, 2011 10:56 AM Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue. I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax: 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow. What made them change their minds? There are many times when we stain > different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for each > block regarding IHC billing, only one unit of billing for each part type no > matter how many blocks are stained? Also IHC "cocktail" stains, such as > PIN4 must now be billed as one unit even though multiple antibodies are > reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC codes > 88342, 88360, and 88361. > > Happy New Year to everyone out there. May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From one_angel_secret <@t> yahoo.com Sat Dec 31 13:52:16 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Dec 31 13:52:22 2011 Subject: [Histonet] RE: NCCI policy update plus copper stain CPT change In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16408454D132C@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E16408454D132C@CHEXCMS10.one.ads.che.org> Message-ID: <1325361136.56885.YahooMailNeo@web112317.mail.gq1.yahoo.com> Like I said before, the organizations I have been with did not choose to do that because of the medically necessity laws that governed that. The Pathologist would have to document the necessity of billing for lets say a keratin done on blocks A1, A2 and A3 on a sentinal node. we always billed for just one?keratin.These are large institutions/path groups?I am refering to.Were they cheating themselves or being medically prudent? Not sure. Also, within the past two years Ive had the pleasure of being subjected to sitting within a group called code correct who helps hospitals stay in compliance. apparently that group did not like the pratice of billing for A1, A2 keratins as well. Bill for A, B, C etc yes. ? Perhaps the Pathologist sitting in this forum can explain it better as to why some Paths who are medical directors choose to not bill for multiple blocks on the same specimen unless they had a out right hard core reason to need it, rare in deed from what Ive seen. Ive had more than one argument on why cant we bill for the clot and make it part type B instead of making it block A2. Ive seen this practice of what im saying over the past 5 years at 2 large hospitals and 2 large private path groups that were the medical directors of the hospital labs. So I can only speak from what Ive seen. ? To say the least is I guess these places are better prepared for this change if in fact they self punished themselves from this practice of billing multiple blocks of same samples. ? but this new billing for using cocktails on one sample and being able to bill one is a whole nother ball game. ? I imagine its going to hit urology groups hard since vendors have been selling the P504s, racemase, 34Be12 cocktails real hard last few years. ? With all this said, I am sitting here laughing at myself. We all can only say what we have personally experianced. and whats funny to me is we think about work too much lol..... ? We should be having a nice cold one. We'll survive somehow with what ever they toss at us. :) ? Happy New Year Histonetters.... Luv Ya! ? Kim D ________________________________ From: "Weems, Joyce" To: Kim Donadio ; Lester Raff MD ; Jesus Ellin ; "histotech@imagesbyhopper.com" Cc: "histonet@lists.utsouthwestern.edu" ; "Webb, Dorothy L" Sent: Saturday, December 31, 2011 1:03 PM Subject: RE: [Histonet] RE: NCCI policy update plus copper stain CPT change For the past two years we have been able to bill immunos and special stains per block. This will be a loss for for the big cases that need multiple blocks stained since the cost is per slide, not specimen. I don't see anything about the special stains tho - doesn't make sense. Global billing is technical and professional charges combined and would have include the separate blocks. NOTE: The CPT code has changed for the copper stain - 88318. It is now in the non-microbial group - 88313. Happy New Year Everyone!! j ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio [one_angel_secret@yahoo.com] Sent: Saturday, December 31, 2011 12:13 PM To: Lester Raff MD; Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update The practice of billing for seperate blocks on one specimen such as A1, A2 and A3 I beleive has long been gone. At least in the organizations I have been with. What seems to be new is they are going after the new trend of using cocktail antibodies. I agree you would think that ASCP and CAP would be on top of this. While i might see the argument that the technical component could be reduced in using these cocktails, I cant see how they think it will benefit anyone for not allowing the fee for each antibody read out on one slide from the Pathologist. I realize most places bill global fee's, so that's a issue that would need to be looked at how to deal with it. But it is a bit unfair when you consider sometimes you only have one tiny little specimen, or maybe even one slide to stain and you need multiple markers. You could even argue that the cost of using cocktails is more expensive for your technical component. If what they do is force labs to go back to using one slide per anibody, I dont feel thats a betterment in healthcare pratcice since sometimes tumor cells are limited to one or two slides per specimen. We've all seen it i'm sure. With all that said, with everything coming down the pipelines at us healthcare professionals, we need to buckle up. It's getting ugly out here.They used to want us (healthcare professionals) to do a lot for a little bit, now its do everything with nothing. There I said it. Kim Donadio ________________________________ From: Lester Raff MD To: Jesus Ellin ; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; "Webb, Dorothy L" Sent: Saturday, December 31, 2011 10:56 AM Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue.? I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax:? 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we stain > different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for each > block regarding IHC billing, only one unit of billing for each part type no > matter how many blocks are stained?? Also IHC "cocktail" stains, such as > PIN4 must now be billed as one unit even though multiple antibodies are > reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC codes > 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From joelleweaver <@t> hotmail.com Sat Dec 31 14:18:08 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Sat Dec 31 14:18:14 2011 Subject: [Histonet] RE: NCCI policy update Message-ID: Yes this link would be great to share. Thanks Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rathborne Toni Date: Sat, 31 Dec 2011 17:21:28 To: ; ; Cc: ; Subject: RE: [Histonet] RE: NCCI policy update Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue.? I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax:? 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update ? Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained?? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Dec 31 14:21:23 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Sat Dec 31 14:21:28 2011 Subject: [Histonet] RE: NCCI policy update Message-ID: Agree, there will be changes with the HITECH legislation, reimburshment meaningful use and coding set revisions on the horizon. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Jesus Ellin Date: Fri, 30 Dec 2011 22:18:17 To: Cc: ; Subject: Re: [Histonet] RE: NCCI policy update Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we stain > different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for each > block regarding IHC billing, only one unit of billing for each part type no > matter how many blocks are stained?? Also IHC "cocktail" stains, such as > PIN4 must now be billed as one unit even though multiple antibodies are > reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC codes > 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Sat Dec 31 16:10:05 2011 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Sat Dec 31 16:12:23 2011 Subject: [Histonet] RE: NCCI policy update References: Message-ID: Here is the link: https://www.cms.gov/NationalCorrectCodInitEd/ This will take you to a folder of zipped documents, one of which is the lab coding rules. Yes this link would be great to share. Thanks Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rathborne Toni Date: Sat, 31 Dec 2011 17:21:28 To: ; ; Cc: ; Subject: RE: [Histonet] RE: NCCI policy update Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue.? I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax:? 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update ? Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained?? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Sat Dec 31 16:13:33 2011 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Sat Dec 31 16:13:58 2011 Subject: FW: [Histonet] RE: NCCI policy update--requested link References: Message-ID: -----Original Message----- From: Lester Raff MD Sent: Sat 12/31/2011 4:10 PM To: joelle weaver ; Rathborne Toni ; JEllin@yumaregional.org ; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu ; Dorothy.L.Webb@HealthPartners.Com Subject: RE: [Histonet] RE: NCCI policy update Here is the link: https://www.cms.gov/NationalCorrectCodInitEd/ This will take you to a folder of zipped documents, one of which is the lab coding rules. Yes this link would be great to share. Thanks Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rathborne Toni Date: Sat, 31 Dec 2011 17:21:28 To: ; ; Cc: ; Subject: RE: [Histonet] RE: NCCI policy update Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue.? I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax:? 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update ? Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow.? What made them change their minds?? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained?? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there.? May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > >? ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Dec 31 16:21:30 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Dec 31 16:21:40 2011 Subject: [Histonet] RE: NCCI policy update In-Reply-To: References: , Message-ID: Thanks so much for posting this, it will streamline getting this information greatly!Joelle Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver Subject: RE: [Histonet] RE: NCCI policy update Date: Sat, 31 Dec 2011 16:10:05 -0600 From: LRaff@uropartners.com To: joelleweaver@hotmail.com; trathborne@somerset-healthcare.com; JEllin@yumaregional.org; histotech@imagesbyhopper.com CC: histonet@lists.utsouthwestern.edu; Dorothy.L.Webb@HealthPartners.Com RE: [Histonet] RE: NCCI policy update Here is the link: https://www.cms.gov/NationalCorrectCodInitEd/ This will take you to a folder of zipped documents, one of which is the lab coding rules. Yes this link would be great to share. Thanks Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rathborne Toni Date: Sat, 31 Dec 2011 17:21:28 To: ; ; Cc: ; Subject: RE: [Histonet] RE: NCCI policy update Do you have the link to this new guideline? The site can be difficult to navigate. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Saturday, December 31, 2011 10:57 AM To: Jesus Ellin; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: RE: [Histonet] RE: NCCI policy update Below is the quote from the new NCCI guidelines that deal with this issue. I am amazed that CAP and ASCP, have not been 1) notifying members and 2) disputing this change. "The unit of service for immunohistochemistry (CPT codes 88342, 88360, 88361) is each antibody(s) stain (procedure) per specimen. If a single immunohistochemical stain (procedure) for one or more antibodies is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service per specimen for an immunohistochemical antibody(s) stain (procedure) even if it contains multiple separately interpretable antibodies" Lester J. Raff, MD Laboratory Medical Director UroPartners Laboratory 2225 Enterprise Dr Suite 2511 Westchester, IL 60154 Phone: 708.486.0076 Fax: 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jesus Ellin Sent: Fri 12/30/2011 4:18 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Webb, Dorothy L Subject: Re: [Histonet] RE: NCCI policy update Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, "histotech@imagesbyhopper.com" wrote: > Wow. What made them change their minds? There are many times when we > stain different blocks of a single specimen and only one lights up. > > I, too, would be interested in an official document stating the change. > > Thanks! > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Friday, December 30, 2011 3:07 PM > To: Martha Ward-Pathology; Webb, Dorothy L; > 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Where can I get the article to show my billing dept/pathologists? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Friday, December 30, 2011 1:13 PM > To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: NCCI policy update > > Oh yes, we are very aware and quite upset at the change! > > > Martha Ward, MT (ASCP) QIHC > Manager, Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 > 336-716-2104 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Friday, December 30, 2011 1:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] NCCI policy update > > Is everyone aware that beginning 1/1/12, we can no longer bill for > each block regarding IHC billing, only one unit of billing for each > part type no matter how many blocks are stained? Also IHC "cocktail" > stains, such as > PIN4 must now be billed as one unit even though multiple antibodies > are reported out. > > Kind of a surprising reversal of the policy set in motion 10/1/2009. > SPECIMEN becomes the unit of service rather than block(s) for IHC > codes 88342, 88360, and 88361. > > Happy New Year to everyone out there. May 2012 find you happiness and > health! > > Dorothy Webb, HT > Regions Histology TS > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: > 12/30/11 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet