[Histonet] Araldite

[Coughlin, Beth]

Anyone have advice for how to get sections from epon blocks that are very soft??
I used a rapid embedding protocol for making epon blocks that I found online.
Unfortunately, I misunderstood the instruction that I was to use araldite alone for the embedding media.
I used the kit that came with the araldite (ddsa and dmp 30).
I didn't actually use the steps for polymerizing recommended below. I just used the early steps for speeding up the processing. I just put my samples in at 60 degrees overnight, as usual.



WASH...............PBS..................2X5 MIN.

1%OsO4 in PBS...........................20 MIN RTEMP.

WASH...............PBS..................2X5 MIN

*70% ETOH...............................3 MIN

95% ETOH................................3 MIN

100% ETOH...............................2X5 MIN

PROPYLENE OXIDE.........................2X5 MIN

1:1 P.O.:ARALDITE 502...................30 MIN (REMOVE Caps)

1:3 " " ...................20 MIN

PURE ARALDITE 502.......................10 MIN

EMBED

CURE IN 75C OVEN FOR 50 MIN. PLACE IN 95C OVEN FOR 50 MIN.

PLACE IN FREEZER AND LET COOL 10 MIN.