[Histonet] Kappa/Lambda ISH background

Johnson, Nacaela Nacaela.Johnson <@t> USONCOLOGY.COM
Fri Aug 5 13:48:44 CDT 2011


I am performing the ISH on the Leica Bond.
 

Thanks,

 

Nacaela Johnson, B.S. HTL (ASCP)CM

Histotechnologist

KCCC Pathology

12000 110th St., Ste. 400

Overland Park, KS 66210

Office:  913-234-0576

Fax:  913-433-7639

Email:  Nacaela.Johnson <@t> USOncology.com

 

________________________________

From: Mark Tarango [mailto:marktarango <@t> gmail.com] 
Sent: Friday, August 05, 2011 10:54 AM
To: Johnson, Nacaela
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Kappa/Lambda ISH background


How are you doing your ISH?  A commercial manual kit, home-made kit,
instrument? 


 
On Fri, Aug 5, 2011 at 8:31 AM, Johnson, Nacaela
<Nacaela.Johnson <@t> usoncology.com> wrote:


	Would anyone be willing to share their protocols for the Kappa
and
	Lambda ISH?  I cannot get rid of the eosinophilic background
staining.
	
	
	Thanks,
	
	
	
	Nacaela Johnson, B.S. HTL (ASCP)CM
	
	Histotechnologist
	
	KCCC Pathology
	
	12000 110th St., Ste. 400
	
	Overland Park, KS 66210
	
	Office:  913-234-0576
	
	Fax:  913-433-7639
	
	Email:  Nacaela.Johnson <@t> USOncology.com
	
	
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