From nicole <@t> dlcjax.com Mon Aug 1 07:44:07 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Mon Aug 1 07:44:15 2011 Subject: [Histonet] Opening in KC, Missouri Message-ID: <2154.208.62.167.196.1312202647.squirrel@webmail.realpages.com> Full time or part time opening in Kansas City, Missouri for a busy Dermatopathology lab. Must be HT or HTL certified. Proficient in all aspects of routine histology. Grossing, coversliping, accessioning, microtonomy, and H&E stains. Please call Tami at 816.584.8100 for immediate consideration. From sforeman <@t> labpath.com Mon Aug 1 11:18:36 2011 From: sforeman <@t> labpath.com (Susan Foreman) Date: Mon Aug 1 11:22:33 2011 Subject: [Histonet] Ventana Benchmark Ultra-Red Problems Message-ID: <007c01cc5066$aa941720$ffbc4560$@com> We have been having an issue lately with a precipitate (possibly bacterial contaminant) using the Ultra View Red Detection Kit that is not showing up on stains using the Ultra View DAB Detection Kit. The red speckles appear on the tissue and also on the glass away from the tissue. We have alerted tech support, done several decontaminations and changed our water from "purified" to "distilled". Has anyone experienced similar problems with the new formulation of the Ultra Red Detection kit or any other similar situations? Any thoughts on the subject would be greatly appreciated. Benchmark Ultra stainer I appreciate your input, Susan KDL Pathology From 41dmb41 <@t> gmail.com Mon Aug 1 11:26:48 2011 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Mon Aug 1 11:27:06 2011 Subject: [Histonet] Ventana Benchmark Ultra-Red Problems In-Reply-To: <007c01cc5066$aa941720$ffbc4560$@com> References: <007c01cc5066$aa941720$ffbc4560$@com> Message-ID: <7EB02928-9A79-4DB7-94BF-C09B857B3098@gmail.com> Have you tried using a different lot of the Ultra View Red... Ours has been working fine, so maybe you got a bad lot? Drew Sent from my iPhone On Aug 1, 2011, at 12:18 PM, "Susan Foreman" wrote: > We have been having an issue lately with a precipitate (possibly bacterial > contaminant) using the Ultra View Red Detection Kit that is not showing up > on stains using the Ultra View DAB Detection Kit. The red speckles appear > on the tissue and also on the glass away from the tissue. We have alerted > tech support, done several decontaminations and changed our water from > "purified" to "distilled". Has anyone experienced similar problems with the > new formulation of the Ultra Red Detection kit or any other similar > situations? Any thoughts on the subject would be greatly appreciated. > Benchmark Ultra stainer > > > > I appreciate your input, > > Susan > > KDL Pathology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sforeman <@t> labpath.com Mon Aug 1 11:24:58 2011 From: sforeman <@t> labpath.com (Susan Foreman) Date: Mon Aug 1 11:28:59 2011 Subject: [Histonet] Ventana Benchmark Ultra-Red Problems In-Reply-To: <7EB02928-9A79-4DB7-94BF-C09B857B3098@gmail.com> References: <007c01cc5066$aa941720$ffbc4560$@com> <7EB02928-9A79-4DB7-94BF-C09B857B3098@gmail.com> Message-ID: <009601cc5067$8e1d7310$aa585930$@com> We have tried both lots of the Ultra View Red -----Original Message----- From: Drew Meyer [mailto:41dmb41@gmail.com] Sent: Monday, August 01, 2011 12:27 PM To: Susan Foreman Cc: Subject: Re: [Histonet] Ventana Benchmark Ultra-Red Problems Have you tried using a different lot of the Ultra View Red... Ours has been working fine, so maybe you got a bad lot? Drew Sent from my iPhone On Aug 1, 2011, at 12:18 PM, "Susan Foreman" wrote: > We have been having an issue lately with a precipitate (possibly bacterial > contaminant) using the Ultra View Red Detection Kit that is not showing up > on stains using the Ultra View DAB Detection Kit. The red speckles appear > on the tissue and also on the glass away from the tissue. We have alerted > tech support, done several decontaminations and changed our water from > "purified" to "distilled". Has anyone experienced similar problems with the > new formulation of the Ultra Red Detection kit or any other similar > situations? Any thoughts on the subject would be greatly appreciated. > Benchmark Ultra stainer > > > > I appreciate your input, > > Susan > > KDL Pathology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anna.Inman <@t> stmarygj.org Mon Aug 1 11:30:51 2011 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Mon Aug 1 11:31:00 2011 Subject: [Histonet] IHC service ?? Message-ID: <2925AE271EAAD440AF48FCCEB8002D091C076D9F@smgmail01.smgj.sclhs.net> Can you tell me what kind of on-site technical service you are accustomed to receiving from Ventana (especially if you NOT in a big city so travel is required)? We have had a terrible time getting a Field service engineer into our lab this year and wonder if that is the "norm" for Ventana these days? For the Dako users ----- what kind of service are you getting? Thank you in advance Anna Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From 41dmb41 <@t> gmail.com Mon Aug 1 12:06:21 2011 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Mon Aug 1 12:06:40 2011 Subject: [Histonet] Ventana Benchmark Ultra-Red Problems In-Reply-To: <009601cc5067$8e1d7310$aa585930$@com> References: <007c01cc5066$aa941720$ffbc4560$@com> <7EB02928-9A79-4DB7-94BF-C09B857B3098@gmail.com> <009601cc5067$8e1d7310$aa585930$@com> Message-ID: Is it appearing on every slide run with that detection regardless of the antibody? What are you using to remove the liquid coverslip? If it's dish-soap, did you just recently change brands or use one with a different formula? Drew Sent from my iPhone On Aug 1, 2011, at 12:24 PM, "Susan Foreman" wrote: > We have tried both lots of the Ultra View Red > > -----Original Message----- > From: Drew Meyer [mailto:41dmb41@gmail.com] > Sent: Monday, August 01, 2011 12:27 PM > To: Susan Foreman > Cc: > Subject: Re: [Histonet] Ventana Benchmark Ultra-Red Problems > > Have you tried using a different lot of the Ultra View Red... Ours has been > working fine, so maybe you got a bad lot? > > Drew > > Sent from my iPhone > > On Aug 1, 2011, at 12:18 PM, "Susan Foreman" wrote: > >> We have been having an issue lately with a precipitate (possibly bacterial >> contaminant) using the Ultra View Red Detection Kit that is not showing up >> on stains using the Ultra View DAB Detection Kit. The red speckles appear >> on the tissue and also on the glass away from the tissue. We have alerted >> tech support, done several decontaminations and changed our water from >> "purified" to "distilled". Has anyone experienced similar problems with > the >> new formulation of the Ultra Red Detection kit or any other similar >> situations? Any thoughts on the subject would be greatly appreciated. >> Benchmark Ultra stainer >> >> >> >> I appreciate your input, >> >> Susan >> >> KDL Pathology >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From candice_camille <@t> yahoo.com Mon Aug 1 12:51:50 2011 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Mon Aug 1 12:51:53 2011 Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections In-Reply-To: References: Message-ID: <1312221110.38910.YahooMailNeo@web125418.mail.ne1.yahoo.com> Hi, we do this stain all the time in our lab. We only use gelatin coated slides. You can purchase them or you can coat them yourselves. Our sections were doing the same thing until we started using the gelatin coated slides. We no longer have that problem. I hope this helps! I remain yours truely, Candice Camille From: "Michael, Susan" To: "histonet@lists.utsouthwestern.edu" Sent: Friday, July 29, 2011 8:11 AM Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections I am having trouble keeping my sections on the slides when I stain with cresyl violet.? These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water.? Into the cresyl violet solution, then 100% to 95% ETOH.? Is it the water?? Is it the slides?? Any suggestions? Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Mon Aug 1 12:55:54 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Mon Aug 1 12:55:59 2011 Subject: [Histonet] Mouse vs. Human cells Message-ID: Hello Histo-hotties! Question: We are working with xenograft tumors in mice. The tumor cells are human in origin. I am trying to come up with some kind of stain that will stain all the mouse cells and none of the human cells. Just to be able to determine if any of the human tumor cells are in a normal looking say liver. I was thinking maybe Ki-67? It doesn't have to be any specific marker, just to be able to see a really blue mouse organ, and then if there are human cells of any type in the organ light up that one cell. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From sdysart <@t> mirnarx.com Mon Aug 1 12:58:10 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Mon Aug 1 12:58:14 2011 Subject: [Histonet] Oops Message-ID: That was backwards =) Don't stain mouse cells...stain human ones Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From Ronald.Houston <@t> nationwidechildrens.org Mon Aug 1 15:13:21 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Aug 1 15:13:31 2011 Subject: [Histonet] C4d nuclear staining? Message-ID: Has anyone ever noticed any nuclear staining with C4d in a renal biopsy? The control stains great, but the patient's tissue exhibits some nuclear staining in what look to be inflammatory cells. There has been a report of granular and some nuclear staining in lung transplants on structures beyond the vasculature but we haven't seen anything like this in a case of renal rejection. Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From lscott <@t> sfcn.org Mon Aug 1 17:02:00 2011 From: lscott <@t> sfcn.org (Scott) Date: Mon Aug 1 17:02:08 2011 Subject: [Histonet] Thanks to Leica service Message-ID: <69EBD4E1-E2AE-4B6A-8041-54B8C56254DC@sfcn.org> Quick note for those who may be interested. I received a message from Leica's service department offering to help with my blade holder issues. Hopefully we can get the problem resolved quickly. Thanks Leica ! Sent from my iPhone From tahseen <@t> brain.net.pk Tue Aug 2 06:39:51 2011 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Tue Aug 2 06:40:00 2011 Subject: [Histonet] uncertainity of results in histopathology Message-ID: <54194.203.135.35.66.1312285191.squirrel@brain.net.pk> Dear all please guide me about uncertainity of results in Histopathology at different steps,including fixation of tissue ,grossing,processing,microtomy,staining,IHC,special staining and interpretation etc. This is the requirement of ISO 15189 CLAUSE:5.6.2 Muhammad Tahseen SKMCH&RC Pakistan From cpostenka <@t> hotmail.com Tue Aug 2 10:16:02 2011 From: cpostenka <@t> hotmail.com (Carl Postenka) Date: Tue Aug 2 10:16:07 2011 Subject: [Histonet] Mouse vs. Human cells In-Reply-To: References: Message-ID: We've had succes identifying human cells in a mouse background using an antibody against human mitochondria (Neomarkers cat#MS-1372P). The primary is a monoclonal mouse anti-human, so we use a Dako ARK kit (#K3954) to eliminate the "mouse on mouse" background. HIER using citrate pH=6.0. H2O2 block. Primary diluted 1/100 (through ARK kit), for 20min @ R/T. -------------------------------------- Carl PostenkaLondon Regional Cancer Program > Date: Mon, 1 Aug 2011 12:55:54 -0500 > From: sdysart@mirnarx.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Mouse vs. Human cells > > Hello Histo-hotties! > > Question: We are working with xenograft tumors in mice. The tumor cells > are human in origin. I am trying to come up with some kind of stain > that will stain all the mouse cells and none of the human cells. Just > to be able to determine if any of the human tumor cells are in a normal > looking say liver. I was thinking maybe Ki-67? It doesn't have to be > any specific marker, just to be able to see a really blue mouse organ, > and then if there are human cells of any type in the organ light up that > one cell. > > Thanks > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Tue Aug 2 10:27:08 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Aug 2 10:27:11 2011 Subject: [Histonet] Mouse vs. Human cells In-Reply-To: References: Message-ID: <1312298828.88360.YahooMailNeo@web130121.mail.mud.yahoo.com> Years ago, we used to distinguish human fibroblasts in mouse tissue with vimentin; which stains human cells quite nicely, but does not cross to mouse. ? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Carl Postenka To: sdysart@mirnarx.com; histonet@lists.utsouthwestern.edu Sent: Tuesday, August 2, 2011 11:16 AM Subject: RE: [Histonet] Mouse vs. Human cells We've had succes identifying human cells in a mouse background using an antibody against human mitochondria (Neomarkers cat#MS-1372P). The primary is a monoclonal mouse anti-human, so we use a Dako ARK kit (#K3954) to eliminate the "mouse on mouse" background. HIER using citrate pH=6.0. H2O2 block. Primary diluted 1/100 (through ARK kit), for 20min @ R/T. -------------------------------------- Carl PostenkaLondon Regional Cancer Program > Date: Mon, 1 Aug 2011 12:55:54 -0500 > From: sdysart@mirnarx.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Mouse vs. Human cells > > Hello Histo-hotties! > > Question: We are working with xenograft tumors in mice.? The tumor cells > are human in origin.? I am trying to come up with some kind of stain > that will stain all the mouse cells and none of the human cells.? Just > to be able to determine if any of the human tumor cells are in a normal > looking say liver.? I was thinking maybe Ki-67?? It doesn't have to be > any specific marker, just to be able to see a really blue mouse organ, > and then if there are human cells of any type in the organ light up that > one cell. > > Thanks > >? > >? > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas? 78744 > > (512)901-0900 ext. 6912 > >? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLashus <@t> pathgroup.com Tue Aug 2 11:19:34 2011 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Tue Aug 2 11:19:54 2011 Subject: [Histonet] Job Opening Message-ID: <197CD0B02A81F94994A285C59C8AE05C07B1B110E4@pgnexchange.pathgroup.com> We are currently seeking a qualified Histotech to join our third shift operation. The Chattanooga area offers many opportunities for work and leisure. PathGroup offers an excellent working environment and benefit package. The candidate we are seeking would need to be a registered HT or eligible and meet the requirements according to CAP and CLIA to gross small specimens. The candidate would alternate the grossing duties with another tech and we would be willing to train the right candidate. If you are interested please send your resume to me at the email address below. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Chattanooga 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 Phone: 423-493-0207 Fax: 423-493-0208 Email: mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From tjay30 <@t> yahoo.com Tue Aug 2 12:05:40 2011 From: tjay30 <@t> yahoo.com (Tim Jay) Date: Tue Aug 2 12:05:58 2011 Subject: [Histonet] Job opening Message-ID: <4834A9F3-36B6-4A1B-AA35-B31F4E59A5EA@yahoo.com> GI path lab in Santa Rosa, CA looking for a certified tech. Great opportunity!!!!!!! Great pay. Flexible hours. Friendly office. Submit resumes to tjay30@yahoo.com. Sent from my iPhone From sdysart <@t> mirnarx.com Tue Aug 2 12:06:38 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Tue Aug 2 12:06:42 2011 Subject: [Histonet] hmm Message-ID: Has anyone ever heard of the company, Stem cells? I have been trying to contact them all morning to order an antibody, but no one ever answers the phone. Little nervous. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From LSebree <@t> uwhealth.org Tue Aug 2 12:13:17 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Aug 2 12:14:29 2011 Subject: [Histonet] IHC service ?? References: <2925AE271EAAD440AF48FCCEB8002D091C076D9F@smgmail01.smgj.sclhs.net> Message-ID: We've had excellent service from Ventana and they do have to travel from usually Chicago up to Madison. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Inman, Anna Sent: Mon 8/1/2011 11:30 AM To: histonet@lists.utsouthwestern.edu. Subject: [Histonet] IHC service ?? Can you tell me what kind of on-site technical service you are accustomed to receiving from Ventana (especially if you NOT in a big city so travel is required)? We have had a terrible time getting a Field service engineer into our lab this year and wonder if that is the "norm" for Ventana these days? For the Dako users ----- what kind of service are you getting? Thank you in advance Anna Anna.Inman@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Tue Aug 2 12:14:24 2011 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue Aug 2 12:14:30 2011 Subject: [Histonet] cell blocks Message-ID: How does everyone process their cell blocks? Currently, we use our biopsy run for smaller cell blocks, but the larger ones we use our routine tissue process. Our lab manager would like us to look at putting all cell blocks, regardless of size, on our biopsy run, to increase turn around time. I have my doubts, since some cell blocks, especially bronch washes and pleural fluids, can be quite large and greasy or mucous-y. What does everyone else do? We use standard 10% NBF, xylene, and paraplast xtra on our processors. We would process on a Leica Peloris. Our standard bx protocol is about 1.5 hours total, and our standard routine protocol is about 7 hours. Thank you! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From mpowers <@t> dpspa.com Tue Aug 2 13:01:03 2011 From: mpowers <@t> dpspa.com (Marian Powers) Date: Tue Aug 2 13:01:07 2011 Subject: [Histonet] SOX-11 Message-ID: Hi: Has anyone had success with antibody SOX-11 and would be willing to share your protocol? Thanks in advance, -- *Marian L. Powers* *Q*uality *L*aboratory *C*onsultants *Doctors Pathology Services * ** c| 302.747.0580 o| 302.677.0000 ext: 110 f | 302.677.0010 From rjbuesa <@t> yahoo.com Tue Aug 2 14:41:38 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 2 14:41:44 2011 Subject: [Histonet] cell blocks In-Reply-To: Message-ID: <1312314098.85498.YahooMailClassic@web65713.mail.ac4.yahoo.com> I always processed cell blocks, regardless of their size, with the regular tissue processing protocol. Ren? J. --- On Tue, 8/2/11, Clare Thornton wrote: From: Clare Thornton Subject: [Histonet] cell blocks To: "'Histonet@lists.utsouthwestern.edu'" Date: Tuesday, August 2, 2011, 1:14 PM How does everyone process their cell blocks?? Currently, we use our biopsy run for smaller cell blocks, but the larger ones we use our routine tissue process.? Our lab manager would like us to look at putting all cell blocks, regardless of size, on our biopsy run, to increase turn around time.? I have my doubts, since some cell blocks, especially bronch washes and pleural fluids, can be quite large and greasy or mucous-y.? What does everyone else do?? We use standard 10% NBF, xylene, and paraplast xtra on our processors.? We would process on a Leica Peloris.? Our standard bx protocol is about 1.5 hours total, and our standard routine protocol is about 7 hours. Thank you! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Tue Aug 2 14:49:18 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Aug 2 14:49:31 2011 Subject: [Histonet] cell blocks In-Reply-To: References: Message-ID: <2493.208.62.167.196.1312314558.squirrel@webmail.realpages.com> you definitly can run them together. I would just test one before I put them all in there, because when they are small they can over process and be very hard and brittle making intrepretation difficult. As far as your larger fatty gooey specimens. Make sure they are no thicker than 3mm. Anything bigger doesnt process well on a normal 7hr cycle. Hope this helps Nicole Tatum HT ASCP How does everyone process their cell blocks? Currently, we use our biopsy > run for smaller cell blocks, but the larger ones we use our routine tissue > process. Our lab manager would like us to look at putting all cell > blocks, regardless of size, on our biopsy run, to increase turn around > time. I have my doubts, since some cell blocks, especially bronch washes > and pleural fluids, can be quite large and greasy or mucous-y. What does > everyone else do? We use standard 10% NBF, xylene, and paraplast xtra on > our processors. We would process on a Leica Peloris. Our standard bx > protocol is about 1.5 hours total, and our standard routine protocol is > about 7 hours. > > Thank you! > > Clare J. Thornton, HTL(ASCP),QIHC > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Tue Aug 2 14:51:09 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Aug 2 14:51:14 2011 Subject: [Histonet] cell blocks In-Reply-To: <1312314098.85498.YahooMailClassic@web65713.mail.ac4.yahoo.com> References: <1312314098.85498.YahooMailClassic@web65713.mail.ac4.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082128C27E@CHEXCMS10.one.ads.che.org> We have a 6 hours program that we process ours on - our assistant works wee hours of the morning and has them ready to start around 6 am. If they don't get on same day, they go on the regular overnight run. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax From: Clare Thornton Subject: [Histonet] cell blocks To: "'Histonet@lists.utsouthwestern.edu'" Date: Tuesday, August 2, 2011, 1:14 PM How does everyone process their cell blocks?? Currently, we use our biopsy run for smaller cell blocks, but the larger ones we use our routine tissue process.? Our lab manager would like us to look at putting all cell blocks, regardless of size, on our biopsy run, to increase turn around time.? I have my doubts, since some cell blocks, especially bronch washes and pleural fluids, can be quite large and greasy or mucous-y.? What does everyone else do?? We use standard 10% NBF, xylene, and paraplast xtra on our processors.? We would process on a Leica Peloris.? Our standard bx protocol is about 1.5 hours total, and our standard routine protocol is about 7 hours. Thank you! Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Luis.Chiriboga <@t> nyumc.org Tue Aug 2 14:54:20 2011 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Tue Aug 2 14:54:24 2011 Subject: [Histonet] Perforin Ab Message-ID: Can anyone recommend a perforin ab for use in FFPE human tissue? Thanks in advance Luis Luis Chiriboga Ph.D OCS Experimental Pathology IHC Core Lab Bellevue Hospital Center Department of Pathology 4w27 (212) 562-4667 Luis.Chiriboga@nyumc.org ------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
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From Vickroy.Jim <@t> mhsil.com  Tue Aug  2 15:41:59 2011
From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim)
Date: Tue Aug  2 15:42:09 2011
Subject: [Histonet] Histotech Position in Springfield, Illinois
Message-ID: <24A4826E8EF0964D86BC5317306F58A55DF71EAE3A@mmc-mail.ad.mhsil.com>

Histotechnician
Job Description
Basic Function: Process tissue and prepare slides for diagnosis by pathologists.
Requirements for the position include:
    High school graduate required.
    HT(ASCP) registered or eligible
    Light physical effort.
Job Location
Springfield, IL US
Position Type
Full-Time/Regular
Hours of Work
9:00 a.m. - 5:30 p.m.
Weekends (specify)
as needed
Exempt or Non-Exempt
Non-Exempt

Our careers website is www.memorialcareers.com

Breanne Kasprzyk?Recruitment Specialist
?Memorial Health System ?701 North First Street? Springfield, IL 62781
?: 217.788.4587?7: 217.788.5539??kasprzyk.breanne@mhsil.com
?choosememorial.org ?: Location
Thank you for choosing Memorial!


James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


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From Tony_Reilly <@t> health.qld.gov.au  Tue Aug  2 17:41:27 2011
From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly)
Date: Tue Aug  2 17:42:20 2011
Subject: [Histonet] uncertainity of results in histopathology
In-Reply-To: <54194.203.135.35.66.1312285191.squirrel@brain.net.pk>
References: <54194.203.135.35.66.1312285191.squirrel@brain.net.pk>
Message-ID: <4E3909B6.411C.0039.1@health.qld.gov.au>

Hi Muhammad
 
I assume you are referring to Measurement of Uncertainty (MOU).  This is only used for quantitative results and not applicable to Histology processing.  The only times I have seen it applied in Anatomic Pathology are for Her2 and white cell counts on Broncho-alveolar lavages. 
 
 EM staff use grids to make measurements on specimens and of course Pathologists use graticules to record measurements on tissue sections.  Our local accreditation body has yet to make a call on these two measurements.
 
regards
Tony
 
 
 

Tony Reilly  B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
_________________________________________________
Clinical and Statewide Services Division| QueenslandHealth
 
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_reilly@health.qld.gov.au
Web:  www.health.qld.gov.au/qhcss/
 
 


>>>  8/2/2011 9:39 pm >>>
Dear all please guide me about uncertainity of results in Histopathology
at different steps,including fixation of tissue
,grossing,processing,microtomy,staining,IHC,special staining and
interpretation etc.
This is the requirement of ISO 15189 CLAUSE:5.6.2
Muhammad Tahseen
SKMCH&RC
Pakistan


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From CrochiereSteve <@t> aol.com  Tue Aug  2 17:46:20 2011
From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com)
Date: Tue Aug  2 17:46:55 2011
Subject: [Histonet] Processing of derm specimens
Message-ID: <6379a.6d5378c5.3b69d83c@aol.com>

I have recently had a problem with my skin specimens being  
"underprocessed". I use a Leica 300ASP. The schedule is as follows:
10% NBF x 2 for 1 hour ea.
80% Reagent Alcohol for 1 hour
95% Reagent Alcohol x 2 for 1 hour each
100% Reagent Alcohol for 1 hour each
Xylene x 3 for 1 hour each
Paraffin x 3 for 1 hour each
 
The specimens are "mushy and swell on the ice"
Any input is welcome.
 
send response to :
_scrochiere@nedlc.com_ (mailto:scrochiere@nedlc.com) 
 
thanks
From liz <@t> premierlab.com  Tue Aug  2 18:00:07 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Tue Aug  2 18:00:12 2011
Subject: [Histonet] Processing of derm specimens
In-Reply-To: <6379a.6d5378c5.3b69d83c@aol.com>
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0687@SBS2K8.premierlab.local>

That processing schedule should be fine for skin samples, we add an additional 100% alcohol step so we have three absolute steps at 1 hour each (I would remove one 95%).  Thickness of your samples is also important they should be around 3mm in thickness if they are thicker than that they may not process properly.  The other thing is that your solutions need to be fresh for samples to process properly.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CrochiereSteve@aol.com
Sent: Tuesday, August 02, 2011 4:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processing of derm specimens

I have recently had a problem with my skin specimens being
"underprocessed". I use a Leica 300ASP. The schedule is as follows:
10% NBF x 2 for 1 hour ea.
80% Reagent Alcohol for 1 hour
95% Reagent Alcohol x 2 for 1 hour each
100% Reagent Alcohol for 1 hour each
Xylene x 3 for 1 hour each
Paraffin x 3 for 1 hour each

The specimens are "mushy and swell on the ice"
Any input is welcome.

send response to :
_scrochiere@nedlc.com_ (mailto:scrochiere@nedlc.com)

thanks
_______________________________________________
Histonet mailing list
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From daniela.bodemer <@t> mcri.edu.au  Tue Aug  2 18:44:52 2011
From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer)
Date: Tue Aug  2 18:44:57 2011
Subject: [Histonet] Reorienting frozen tissue
Message-ID: <9DF797D618351549B984596F01A1FE1D01CE45B5@murmx.mcri.edu.au>

Hi all,

 

I would like to know if it is possible and how to reorient or reembedd
human tissue, that has been frozen at -80C in OCT. 

 

Many thanks,

 

Daniela Bodemer 

Research Assistant

Surgical Research, Infection and Immunity

 

Murdoch Childrens Research Institute

The Royal Children's Hospital

Flemington Road Parkville Victoria 3052 Australia 

T 03 9345 5930     T (03 9345 4116)     

E daniela.bodemer@mcri.edu.au  

www.mcri.edu.au  

 

This e-mail and any attachments to it (the "Communication") are, unless
otherwise stated, confidential, may contain copyright material and is
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Communication are those of the individual sender only, unless expressly
stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21
006 566 972 or any of its related entities. MCRI does not accept
liability in connection with the integrity of or errors in the
Communication, computer virus, data corruption, interference or delay
arising from or in respect of the Communication. 

P      Please consider the environment before printing this email

 


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From sparker <@t> vt.edu  Tue Aug  2 21:53:38 2011
From: sparker <@t> vt.edu (Scott Parker)
Date: Tue Aug  2 21:53:42 2011
Subject: [Histonet] Manual embedding
Message-ID: 

Dear Histonetters:

I am interested in acquiring a pitcher and heating jacket for melting and
pouring paraffin during manual embedding. My work is relatively low volume
and in a university research lab setting so I am trying to avoid purchasing
an expensive embedding station. Can anyone recommend an honest supplier of
used histology equipment that might be able to provide me with this item?

Thank you for your expertise!

Scott L. Parker
From MAUGER <@t> email.chop.edu  Wed Aug  3 06:19:01 2011
From: MAUGER <@t> email.chop.edu (Mauger, Joanne)
Date: Wed Aug  3 06:19:09 2011
Subject: [Histonet] RE: Perforin Ab
In-Reply-To: 
References: 
Message-ID: <443F5B475A9BF647AB962E834884EBAD27EC1A9510@EX7CCRPW03V1.chop.edu>

Hi Luis,
We use perforin from Thermo-Fisher, #MS-1834.
Jo Mauger
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis
Sent: Tuesday, August 02, 2011 3:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Perforin Ab

Can anyone recommend a perforin ab for use in FFPE human tissue?
Thanks in advance
Luis


Luis Chiriboga Ph.D
OCS Experimental Pathology IHC Core Lab
Bellevue Hospital Center
Department of Pathology 4w27
(212) 562-4667
Luis.Chiriboga@nyumc.org
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From kmerriam2003 <@t> yahoo.com  Wed Aug  3 07:26:23 2011
From: kmerriam2003 <@t> yahoo.com (Kim Merriam)
Date: Wed Aug  3 07:26:28 2011
Subject: [Histonet] Reorienting frozen tissue
In-Reply-To: <9DF797D618351549B984596F01A1FE1D01CE45B5@murmx.mcri.edu.au>
References: <9DF797D618351549B984596F01A1FE1D01CE45B5@murmx.mcri.edu.au>
Message-ID: <1312374383.80758.YahooMailNeo@web130103.mail.mud.yahoo.com>

Whenever I have to reembed an OCT block, I leave it out at RT for just a couple of minutes to let it that a little bit.? Just take a razor blade and cut out the tissue (leaving some OCT around it); re-oreint it in a mold with RT OCT and then freeze it.? Don't freeze it too fast, you want the old OCT to melt a little bit with the new OCT, that way you will have a block without any seams in it.
?
Good luck!
Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

From: Daniela Bodemer 
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, August 2, 2011 7:44 PM
Subject: [Histonet] Reorienting frozen tissue

Hi all,



I would like to know if it is possible and how to reorient or reembedd
human tissue, that has been frozen at -80C in OCT. 



Many thanks,



Daniela Bodemer 

Research Assistant

Surgical Research, Infection and Immunity



Murdoch Childrens Research Institute

The Royal Children's Hospital

Flemington Road Parkville Victoria 3052 Australia 

T 03 9345 5930? ? T (03 9345 4116)? ? 

E daniela.bodemer@mcri.edu.au  

www.mcri.edu.au  



This e-mail and any attachments to it (the "Communication") are, unless
otherwise stated, confidential, may contain copyright material and is
for the use only of the intended recipient. If you receive the
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e-mail, delete the Communication and the return e-mail, and do not read,
copy, retransmit or otherwise deal with it. Any views expressed in the
Communication are those of the individual sender only, unless expressly
stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21
006 566 972 or any of its related entities. MCRI does not accept
liability in connection with the integrity of or errors in the
Communication, computer virus, data corruption, interference or delay
arising from or in respect of the Communication. 

P? ? ? Please consider the environment before printing this email




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From Kim.Kolman <@t> va.gov  Wed Aug  3 07:57:58 2011
From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.)
Date: Wed Aug  3 07:58:06 2011
Subject: [Histonet] paraffin temps
Message-ID: <9C32F30B6662D74A8419DDDB7E66656A0639CE8A@VHAV15MSGA1.v15.med.va.gov>

How do you all handle adjustments in paraffin temps depending on
different brands of paraffins? 

 

Last time I ordered, I received a different brand of Paraplast than my
usual and it is obvious that the temp on the tissue processor needs to
be adjusted up, as the paraffin is collecting in large amounts all over
the top of the cassette basket and is difficult to clean.  To adjust the
temps on the paraffin baths works just fine (as in up to 60 degrees),
which raises the actual paraffin temps up to 62-64 degrees and is then
back to easier melting and cleaning, but the manufacturer packaging
states 'do not heat over 62 degrees'.   

 

I can revise my QC maintenance sheets to alter the temperature ranges so
I am not constantly out of range; however there is the issue of the
packaging....

 

Can't wait to get all this brand used up; I want my McCormick back!

 

Kimberly D. Kolman, HT, (ASCP)

Diagnostics 115 

VA Eastern Kansas Health Care System

4101 S. 4th St. Trfwy.

Leavenworth, KS 66048

ph: 913-682-2000 x 52537/52539

 

 

From rjbuesa <@t> yahoo.com  Wed Aug  3 08:20:31 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Aug  3 08:20:35 2011
Subject: [Histonet] Reorienting frozen tissue
In-Reply-To: <9DF797D618351549B984596F01A1FE1D01CE45B5@murmx.mcri.edu.au>
Message-ID: <1312377631.85779.YahooMailClassic@web65715.mail.ac4.yahoo.com>

Thaw and re-embed in OCT as desired. Freeze again.
Ren? J.

--- On Tue, 8/2/11, Daniela Bodemer  wrote:


From: Daniela Bodemer 
Subject: [Histonet] Reorienting frozen tissue
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, August 2, 2011, 7:44 PM


Hi all,



I would like to know if it is possible and how to reorient or reembedd
human tissue, that has been frozen at -80C in OCT. 



Many thanks,



Daniela Bodemer 

Research Assistant

Surgical Research, Infection and Immunity



Murdoch Childrens Research Institute

The Royal Children's Hospital

Flemington Road Parkville Victoria 3052 Australia 

T 03 9345 5930? ???T (03 9345 4116)? ???

E daniela.bodemer@mcri.edu.au  

www.mcri.edu.au  



This e-mail and any attachments to it (the "Communication") are, unless
otherwise stated, confidential, may contain copyright material and is
for the use only of the intended recipient. If you receive the
Communication in error, please notify the sender immediately by return
e-mail, delete the Communication and the return e-mail, and do not read,
copy, retransmit or otherwise deal with it. Any views expressed in the
Communication are those of the individual sender only, unless expressly
stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21
006 566 972 or any of its related entities. MCRI does not accept
liability in connection with the integrity of or errors in the
Communication, computer virus, data corruption, interference or delay
arising from or in respect of the Communication. 

P? ? ? Please consider the environment before printing this email




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From rjbuesa <@t> yahoo.com  Wed Aug  3 08:24:37 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Aug  3 08:24:42 2011
Subject: [Histonet] paraffin temps
In-Reply-To: <9C32F30B6662D74A8419DDDB7E66656A0639CE8A@VHAV15MSGA1.v15.med.va.gov>
Message-ID: <1312377877.35283.YahooMailClassic@web65709.mail.ac4.yahoo.com>

The brand of the paraffin has nothing to do with the melting temperature, which depends on the paraffin polymers it contains.
Each paraffin will have a melting point value in the package and will melt at that temperature. You can use a higher temperature also but not a lower one.
Ren? J.

--- On Wed, 8/3/11, Kolman, Kimberly D.  wrote:


From: Kolman, Kimberly D. 
Subject: [Histonet] paraffin temps
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, August 3, 2011, 8:57 AM


How do you all handle adjustments in paraffin temps depending on
different brands of paraffins? 



Last time I ordered, I received a different brand of Paraplast than my
usual and it is obvious that the temp on the tissue processor needs to
be adjusted up, as the paraffin is collecting in large amounts all over
the top of the cassette basket and is difficult to clean.? To adjust the
temps on the paraffin baths works just fine (as in up to 60 degrees),
which raises the actual paraffin temps up to 62-64 degrees and is then
back to easier melting and cleaning, but the manufacturer packaging
states 'do not heat over 62 degrees'.???



I can revise my QC maintenance sheets to alter the temperature ranges so
I am not constantly out of range; however there is the issue of the
packaging....



Can't wait to get all this brand used up; I want my McCormick back!



Kimberly D. Kolman, HT, (ASCP)

Diagnostics 115 

VA Eastern Kansas Health Care System

4101 S. 4th St. Trfwy.

Leavenworth, KS 66048

ph: 913-682-2000 x 52537/52539





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From nicole <@t> dlcjax.com  Wed Aug  3 10:36:41 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Wed Aug  3 10:36:49 2011
Subject: [Histonet] Processing of derm specimens
In-Reply-To: <6379a.6d5378c5.3b69d83c@aol.com>
References: <6379a.6d5378c5.3b69d83c@aol.com>
Message-ID: <4295.208.62.167.196.1312385801.squirrel@webmail.realpages.com>

How many 100%  do you have.  I have 3. This is where the dehydration comes
in. The 100 takes all the mositure out of the specimen. So this step is
critical. Water and xylene are not soluable so if the specimens are not
getting dehydrated properly, the xylene will not penertate the specimens
either. Next is make sure your specimen are not thicker then 3mm.
Espceially lipomas and cyst. Try to cut them as thin as possible. Note,
the caseous inside of a cyst will likely not process and usually expoldes
on a water bath. Next, make sure the specimen cassettes are propely placed
inside the tissue rack. If flow between cassettes is restricted, a portion
of a specimen could be raw.

Hope this helps.

Nicole Tatum HT ASCP

I have recently had a problem with my skin specimens being
> "underprocessed". I use a Leica 300ASP. The schedule is as follows:
> 10% NBF x 2 for 1 hour ea.
> 80% Reagent Alcohol for 1 hour
> 95% Reagent Alcohol x 2 for 1 hour each
> 100% Reagent Alcohol for 1 hour each
> Xylene x 3 for 1 hour each
> Paraffin x 3 for 1 hour each
>
> The specimens are "mushy and swell on the ice"
> Any input is welcome.
>
> send response to :
> _scrochiere@nedlc.com_ (mailto:scrochiere@nedlc.com)
>
> thanks
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From Valerie.Hannen <@t> parrishmed.com  Wed Aug  3 10:57:06 2011
From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie)
Date: Wed Aug  3 10:57:10 2011
Subject: [Histonet] Immuno. confusion
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3229250FAD4@ISEXSTORE03.parrishmed.local>

Hi folks...

I am going to try to revamp out Immuno. procedure and am looking at a couple of spec. sheets from our primary vendor. I am gettin conflicting information...so I am turning to you all for help.

When doing immuno's that require either HIER or enzyme digestion, do you perform the retrieval first or do you do the "peroxide" step first??
One spec. sheets says retrieve then block endogenous peroxidase...the other says block endogenous peroxidase then do the HIER or enzyme digestion..which is correct??

One other question that I have is...what do you use to enhance your stain?? The enhancing solution that we were using has been discontinued.

Thanks so much!!

Valerie Hannen,MLT(ASCP), HTL,SU(FL)
Histotechnologist
Parrish Medical Center
951 N. Washington Avenue
Titusville, Florida 32796
(321) 268-6111 ext. 7506



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under applicable law. If the reader of this email is not the
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From rjbuesa <@t> yahoo.com  Wed Aug  3 11:09:21 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Aug  3 11:09:25 2011
Subject: [Histonet] Immuno. confusion
In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3229250FAD4@ISEXSTORE03.parrishmed.local>
Message-ID: <1312387761.79218.YahooMailClassic@web65706.mail.ac4.yahoo.com>

If you fix with formaldehyde, HIER will eliminate the cross link and that step is necessary BEFORE you can apply the peroxide. The enzyme (peroxidase) is also a protein and has to be "open" to receive the H202.
The same if you want to apply enzymatic digestion, HIER has to be done first.
HIER will remove the formaldehyde cross linking and this step is required before any other in the IHC protocol.
Ren? J.

--- On Wed, 8/3/11, Hannen, Valerie  wrote:


From: Hannen, Valerie 
Subject: [Histonet] Immuno. confusion
To: "histonet@lists.utsouthwestern.edu" 
Date: Wednesday, August 3, 2011, 11:57 AM


Hi folks...

I am going to try to revamp out Immuno. procedure and am looking at a couple of spec. sheets from our primary vendor. I am gettin conflicting information...so I am turning to you all for help.

When doing immuno's that require either HIER or enzyme digestion, do you perform the retrieval first or do you do the "peroxide" step first??
One spec. sheets says retrieve then block endogenous peroxidase...the other says block endogenous peroxidase then do the HIER or enzyme digestion..which is correct??

One other question that I have is...what do you use to enhance your stain?? The enhancing solution that we were using has been discontinued.

Thanks so much!!

Valerie Hannen,MLT(ASCP), HTL,SU(FL)
Histotechnologist
Parrish Medical Center
951 N. Washington Avenue
Titusville, Florida 32796
(321) 268-6111 ext. 7506



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hereby notified that any dissemination, distribution, or
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From theresec <@t> slonepartners.com  Wed Aug  3 11:16:38 2011
From: theresec <@t> slonepartners.com (Therese Cook)
Date: Wed Aug  3 11:16:42 2011
Subject: [Histonet] Seeking a Histology Manager in CT
Message-ID: 


   Slone  Partners   laboratory client in Connecticut.
   

   
The  successful  candidate  will  have  experience  with  workflow   redesign  and be great at managing change. The manager will oversee 38
   people,   plus.
      
Qualified  candidates  will  have  a B.S. degree and either HT/HTL   certification,  with  5 years of supervisory experience, preferably in
   a   

   
Special features   the opportunity   

   
If   for  this   [1]theresec@slonepartners.com.
   

   
If  you  have experience in the diagnostic laboratory industry   wish  to be considered for other roles, please forward your resume to   Tara Kochis at [2]tara@slonepartners.com.
   

   
All inquiries are kept confidential.

References

   1. 3D"mailto:theresec@slonepartners.com"
   2. 3D"mailto:tara@slonepartners.com"
From darcyb <@t> slonepartners.com  Wed Aug  3 11:17:55 2011
From: darcyb <@t> slonepartners.com (Darcy Bloch)
Date: Wed Aug  3 11:17:58 2011
Subject: [Histonet] Seeking a Histotechnologist in NE TX
Message-ID: 


   Slone   Partners   Histotechnologist   for   our  hospital-based   Northeast Texas.
   

   
Qualified   life science and   

   
The  ideal  candidate  will   embedding, cutting, staining and other   in a fast paced working environment.
   

   
If  you  meet these qualifications   for  this  opportunity  please  submit  your   [1]darcyb@slonepartners.com.
   

   
If  you  have experience in the diagnostic laboratory industry   wish  to be considered for other roles, please forward your resume to   Tara Kochis at [2]tara@slonepartners.com.
   

   
All inquiries are kept confidential.

References

   1. 3D"mailto:Slone%20Partners%20seeks%20a%20Laboratory%20Sales%20Representatives%20based%20in%20New%20Jersey.%20%20This%20driven%20and%20highly%20motivated%20individual%20will%20be%20responsible%20for%20generating%20new%20business%20in%20the%20competitive%20physician%20market%20in%20the%20state%20of%20New%20Jersey.%20%20Candidates%20must%20be%20able%20to%20prospect%20and%20close%20new%20business%20for%20a%20clinical%20diagnostic%20laboratory%20and%20have%20excellent%20communication%20skills,%20a%20positive%20attitude,%20and%20self-motivation.%20%20This%20position%20offers%20an%20extremely%20lucrative%20compensation%20package%20for%20someone%20with%20a%20%22hunter%20mentality%22%20and%20desire%20to%20succeed.%20%20Experience%20in%20the%20diagnostic%20laboratory%20industry%20is%20a%20plus.%20%20Please%20note:%20%20A%20proven%20track%20record%20of%20sales%20success%20is%20required.%20%20If%20you%20meet%20these%20qualifications%20and%20would%20like%
   2. 3D"mailto:tara@slonepartners.com"
From darcyb <@t> slonepartners.com  Wed Aug  3 11:19:03 2011
From: darcyb <@t> slonepartners.com (Darcy Bloch)
Date: Wed Aug  3 11:19:06 2011
Subject: [Histonet] Seeking Histotechnologist in Upstate NY
Message-ID: 


   Slone   graduates are   

   
Located  in upstate NY, this large university-based   is  looking for talented histotechs who can work a flexible   (starting at 5:00am, or 6am or 7am -working 81/2 hours shift   hr  for  lunch) to join their dynamic team. If you are reliable,   pride  in  quality  work,  are  dedicated  to patient care, and have a
   desire   might be the   

   
The  ideal  candidates   science  or  higher and hold HT or   will   also   consider   experienced   MTs  that   transitioning  to histology. All candidates must either   license or be eligible for NY licensure.
   

   
Special   features   about   this   position:   Our  client   significant   opportunities   for   advancement  and  has  outstanding
   benefits.
   

   
If   for    this   [1]darcyb@slonepartners.com.
   

   
If  you  have experience   wish  to be considered for other   Tara Kochis at [2]tara@slonepartners.com.
   

   
All inquiries are kept confidential.

References

   1. 3D"mailto:darcyb@slonepartners.com"
   2. 3D"mailto:tara@slonepartners.com"
From rsrichmond <@t> gmail.com  Wed Aug  3 11:28:44 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Wed Aug  3 11:28:48 2011
Subject: [Histonet] Dyes used in Crayola wax crayons
Message-ID: 

I started what turned into a remarkably lengthy and diverse Facebook
thread about Crayola? brand wax crayons. Some people use them to dye
fabrics, particularly cotton T-shirts.

Poking around the Web, I get the idea that Crayolas are colored by
Procion MX reactive cotton dyes, which come in a vast variety of
colors. The dyes are suspended in particulate form in the wax, rather
than dissolved. Some people fix the dye in the fabric after dyeing by
soaking the fabric in salt water, a practice that certainly sounds
like reactive cotton dyes.

Anyone know if this information is correct? Obviously reactive cotton
dyes don't have much use in histology.

If anybody wants to friend me on Facebook, I'm the Bob Richmond with
"Harvard" after his name.

Bob Richmond
Samurai Pathologist
Knoxville TN

From relia1 <@t> earthlink.net  Wed Aug  3 11:30:53 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Wed Aug  3 11:30:54 2011
Subject: [Histonet] RELIA Histology Careers Bulletin 8/3/2011 How are you
	staying cool during these dog days of Summer?
Message-ID: <7A9EA8CAACE14855BAF03DCC0784FAB8@ownerf1abaad51>

Hi Histonetters!,

I hope you are having a great day!  ! What are you doing to stay cool
during these dog days of summer?
I am sipping icy cold drinks and spending as much time as possible at
the beach and in the Air Conditioning!!!  If you are contemplating
making a job change let me help while you kick back relax and enjoy a
glass of iced tea or lemonade in the shade.  I can keep you posted on
opportunities as they come open, assist you with your resume and coach
you through the interview process.

All of the positions I work with are fulltime 40 hour per week positions
with top hospitals, labs and doctors offices.  My clients offer
excellent compensation including competitive salaries, great benefits,
relocation assistance and in some cases sign on bonuses.  My services
are FREE of charge to you.  All of my fees are paid by my clients, the
facilities that I represent.  I work with companies nationwide that are
in need of histology supervisors, histotechnologists and
histotechnicians.  




Here is a list of my most exciting current openings:



HISTOLOGY/PATHOLOGY  MANAGEMENT

TX ? Anatomic Pathology Laboratory Manager - Austin

MA ? Night Shift Supervisor ? Boston area

NC ? Laboratory Manager ? Histology/Cytology - Asheville

 

HISTOTECHS

FL ? Evening Lead DermpathTech - Miami

NC ? Afternoon Shift Grossing Histotechnologist-Charlotte

TX ? Histology Technician - Austin

LA ? Night Shift Histology Tech - Lafayette

NC ? Day shift - Asheville

FL ? Mohs Histotech ? Sarasota

MD ? Night Shift Grossing Histotechnologist - Baltimore

MA ? Day shift ? Cape Cod 

OTHER OPPORTUNITIES

Lead Grossing Tech ? Austin, TX

Path Assistant ? Charlotte, NC

Specimen Processing Manager ? Portland, ME

If you are interested in any of these positions please call me at
866-607-3542 or e-mail me at relia1@earthlink.net  If you would like you
can e-mail me your resume and a number where I can reach you at a time
that is convenient for you.  If you are interested in looking into new
job opportunities in other areas that are not mentioned above please
contact me as well.  I work with facilities nationwide and I will keep
your resume confidential.  I will only represent you to jobs you tell me
you are interested in looking into.



Remember. It never hurts to keep an eye open even if you are happy in
your present job.


Also if you know anyone else that might be interested I would really
appreciate it if you would pass my information along to them as well.  I
offer a 500.00 referral fee if you refer someone that I place!

Thank - Pam - 866-607-3542 (866-60RELIA)


Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.comPamBarkerRELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From tammy <@t> surgicalpathlabs.com  Wed Aug  3 11:35:25 2011
From: tammy <@t> surgicalpathlabs.com (Tammy de Leon)
Date: Wed Aug  3 11:39:11 2011
Subject: [Histonet] job openings
Message-ID: 

 

 

If you're looking for a friendly environment, a new state-of-the-art
facility and an employer of choice, SPL is the place for you!! We are
currently looking for qualified Histotechnicians/Histotechnologists!

 

Histo candidates should be an HTL or HT (ASCP) or equivalent.

Primary responsibilities include on-site frozen sections including mobile
laboratory units.

 

SPL is CAP accredited, offers competitive pay, and a comprehensive benefits
package.  SPL pays 100% of employee premiums for  Medical, Dental, LTD and
Life!  We also offer a Retirement Plan & Supplemental Insurance.

 

Please forward resume to

Tammy de Leon

tammy@surgicalpathlabs.com

Surgical Pathology Laboratory

800-304-1066

8455 66th Street N

Pinellas Park, Florida 33781

www.surgicalpathlabs.com

 

From Ashley.Troutman <@t> Vanderbilt.Edu  Wed Aug  3 11:49:40 2011
From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A)
Date: Wed Aug  3 11:49:47 2011
Subject: [Histonet] Immuno. confusion
Message-ID: <7B310892042DA74CB3590053F424CFE6143EE301B7@ITS-HCWNEM06.ds.Vanderbilt.edu>

Hi Valerie,

The peroxidase blocking step is to prevent endogenous peroxidase activity that will cause a false positive stemming from your application of DAB (the substrate for your DAB solution is hydrogen peroxide).  You can essentially put this blocking step at any point in your stain (following deparaffinization) and before your application of DAB.  I have run this step at varying points in my procedures and they all work fine.  Some antibodies prefer you do this step before primary application and others prior to DAB, but for most stains, it doesn't matter too much.

As for an enhancer, you can make a copper sulfate solution (5mg/mL in buffer).  Or you can try DAB Enhancer or DAB Sparkle from Biocare.

Good luck,

Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN  37232

From: Hannen, Valerie 
Subject: [Histonet] Immuno. confusion
To: "histonet@lists.utsouthwestern.edu" 
Date: Wednesday, August 3, 2011, 11:57 AM


Hi folks...

I am going to try to revamp out Immuno. procedure and am looking at a couple of spec. sheets from our primary vendor. I am gettin conflicting information...so I am turning to you all for help.

When doing immuno's that require either HIER or enzyme digestion, do you perform the retrieval first or do you do the "peroxide" step first??
One spec. sheets says retrieve then block endogenous peroxidase...the other says block endogenous peroxidase then do the HIER or enzyme digestion..which is correct??

One other question that I have is...what do you use to enhance your stain?? The enhancing solution that we were using has been discontinued.

Thanks so much!!

Valerie Hannen,MLT(ASCP), HTL,SU(FL)
Histotechnologist
Parrish Medical Center
951 N. Washington Avenue
Titusville, Florida 32796
(321) 268-6111 ext. 7506

From amario3 <@t> uic.edu  Wed Aug  3 12:20:17 2011
From: amario3 <@t> uic.edu (Andrea Marion)
Date: Wed Aug  3 12:20:21 2011
Subject: [Histonet] Manual embedding
Message-ID: 

Hi Scott,

I work in a research lab that does manual embedding too. I looked into
purchasing a paraffin pitcher, but it was too expensive for us. Our
makeshift embedding center is comprised of:

1) 3 thick-walled plastic 500 ml beakers covered in a double layer of foil
(you could use glass, but it gets slippery with melted paraffin on it)
2) a heating block with the surface covered in aluminum foil
3) a water bath
4) a set of weighted rings

We remove most of the water from the bath (otherwise the paraffin beakers
want to float), and use the beakers to store melted paraffin. Two are for
infiltrating, one stores clean paraffin for embedding. The weighted rings
are placed on top of the beakers, supported by the foil. The foil also
keeps water from getting into the paraffin.

After infiltration, I use a plastic mold, place it on the covered heating
block (held at ~65 degree C), fill with paraffin, then place and orient
the specimen in the mold. The blocks sit at room temperature until they
are mostly set, then stored in a 4 degree fridge to finish hardening.

The only problem we have had is occasionally getting water in the paraffin
from condensation. This hasn't been a problem since removing the lid of
the water bath. If it happens, you will see the water sitting under the
melted paraffin. We either replace the paraffin, place plastic cassettes
at the bottom of the beaker so the samples can sit above the level of the
water, or use disposable plastic pipettes to remove the water.

I have also used a dry incubator set to 60 degrees with the same
equipment. It is slightly more convenient, but the paraffin takes longer
the melt than in the water bath (heat exchange is slower).

I also occasionally use a metal pitcher for storing paraffin either in the
incubator or water bath (heating jacket has not been necessary for us).
You can purchase these very cheaply from Amazon - look for stainless steel
milk frothing pitchers that are used for making coffee drinks.

Good luck!

Andrea Marion
Graduate Student
University of Illinois at Chicago


Dear Histonetters:

I am interested in acquiring a pitcher and heating jacket for melting and
pouring paraffin during manual embedding. My work is relatively low volume
and in a university research lab setting so I am trying to avoid purchasing
an expensive embedding station. Can anyone recommend an honest supplier of
used histology equipment that might be able to provide me with this item?

Thank you for your expertise!

Scott L. Parker


From pruegg <@t> ihctech.net  Wed Aug  3 13:19:40 2011
From: pruegg <@t> ihctech.net (pruegg@ihctech.net)
Date: Wed Aug  3 13:19:45 2011
Subject: [Histonet] Immuno. confusion
Message-ID: <20110803111940.f86bd30e73b823f57b516b5451216a98.0652434256.wbe@email00.secureserver.net>


   I have seen this done many ways, some people have h202 at th   their  depara  setup  if  doing  that  off line.  For the Leica Bon   instrument  we were advised to do h202 block just before the DAB and i
   hav   years  h   affected  by  it so   may  be.   There  was    needed to be h202 blocked be   work  if  you  did not do the block b   show that each ab has to be handled sp
   




   


   A  little  aside  here and techni   having  trouble  with  the  Leica  ap/red  ki   advised  by  Jim Burchette not Leica to use H202    substrate chromogen and that has really improved the ap   for  us  which  we use a lot on derm samples.  I know    phos uses levamisol as a block and not h202 but for some rea   works so I don't argue with it.

   




   


   R
   


   Patsy

   




   


     


   --------   Original   Message   --------Subject:   [Histonet]  Immuno.
   confusion
   From: "Hannen, Valerie"  ricerca.com  Wed Aug  3 13:36:47 2011
From: carol.wilson <@t> ricerca.com (Wilson, Carol)
Date: Wed Aug  3 13:37:23 2011
Subject: [Histonet] FDA Reagent Label Requirements for Richard allan Flex
	alcohols
Message-ID: <19848F1C0886A5409C729CC2136EDAFF0557388F67@IAD2MBX09.mex02.mlsrvr.com>

Hi All,
I currently got inspected by my QA dept. and they complained that our Flex alcohols did not have storage requirements on their labels, is anyone using the new Flex alcohols in the larger bottles and if so, do they have the storage requirements on the labels.  If not, are there any GLP labs out there that add a label to their alcohols when they are received stating storage requirements?  Looking for ideas to have a simple fix for this.
Thanks,
Carol

Carol Wilson, HT(ASCP)
Lead Technician/Histology
Ricerca Biosciences, LLC

From arme <@t> optonline.net  Wed Aug  3 14:15:17 2011
From: arme <@t> optonline.net (American Resource Medical)
Date: Wed Aug  3 14:15:25 2011
Subject: [Histonet] For Sale - Mopec MB100 Countertop Grossing Station
Message-ID: <008f01cc5211$b1a69db0$14f3d910$@net>

For Sale - Mopec MB100 Countertop Grossing Station
 
This is a used piece in excellent condition and half the price of new. 
 
 
 
http://media.mopec.com/media/pdf/Catalog2007smaller159.pdf
 
 
 
This is a countertop unit.   
 
 
American ReSource Medical 
 
P: 201.833.1550

 

From estellamireles <@t> gmail.com  Wed Aug  3 15:29:42 2011
From: estellamireles <@t> gmail.com (Stella Mireles)
Date: Wed Aug  3 15:29:45 2011
Subject: [Histonet] Manual embedding
In-Reply-To: 
References: 
Message-ID: 

Andrea,
I have send you an idea that might work for you. Check your email.
Hope this will help.
From rjbuesa <@t> yahoo.com  Wed Aug  3 15:37:44 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Aug  3 15:37:48 2011
Subject: [Histonet] Manual embedding
In-Reply-To: 
Message-ID: <1312403864.11362.YahooMailClassic@web65704.mail.ac4.yahoo.com>

Stella:
Just remember that the mission of HistoNet is not only to reply to specific queries, but to do so in a way that we all could benefit.
Instead of sending a good idea to somebody privately, you should let everybody know about it.
Ren? J.

--- On Wed, 8/3/11, Stella Mireles  wrote:


From: Stella Mireles 
Subject: Re: [Histonet] Manual embedding
To: "Andrea Marion" 
Cc: histonet@lists.utsouthwestern.edu
Date: Wednesday, August 3, 2011, 4:29 PM


Andrea,
I have send you an idea that might work for you. Check your email.
Hope this will help.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From estellamireles <@t> gmail.com  Wed Aug  3 16:03:08 2011
From: estellamireles <@t> gmail.com (Stella Mireles)
Date: Wed Aug  3 16:03:13 2011
Subject: [Histonet] Manual embedding
In-Reply-To: 
References: 
Message-ID: 

A couple of ideas:
Suggest looking for a used paraffin bath from an outside vendor. #2 Idea: If
anyone knows of a lab that is closing up, they might be able to contact
Andrea and pass that info. I know when a lab is closing up, storage of
equipment becomes burdensome.
From ruppert.amysue <@t> marshfieldclinic.org  Wed Aug  3 16:48:03 2011
From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue)
Date: Wed Aug  3 16:48:16 2011
Subject: [Histonet] Thanks to Leica service&In-Reply-To=
Message-ID: <201108032147.p73LltPw029952@mailhost2.mfldclin.edu>

One point to bring up, and I am sure that Leica service will do this, is the brand of knives you are using. Different brands have slightly different thickness. This is not related to the height of the blade. We have found that we need to use the blades that are used by our service company to calibrate the microtomes to have the correct tension in the holder and not any problems with the quality of the sections. 
 good luck, seems you are on the right track though.
amysue ruppert

______________________________________________________________________
The contents of this message may contain private, protected and/or privileged information.  If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.  Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.  Thank you for your cooperation.
From liz <@t> premierlab.com  Wed Aug  3 16:55:39 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Wed Aug  3 16:55:43 2011
Subject: [Histonet] microtome service/repair Denver area
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA06B8@SBS2K8.premierlab.local>

Hello everyone

I was wondering if anyone has any recommendations for a vendor that does yearly service and repair on Leica microtomes and cryostats.  We have been working with one vendor for years now and always have been happy with their service but they have become very expensive, any suggestions would be appreciated.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

From ruppert.amysue <@t> marshfieldclinic.org  Wed Aug  3 17:10:37 2011
From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue)
Date: Wed Aug  3 17:10:45 2011
Subject: [Histonet] Leica Service Technician&In-Reply-To=
Message-ID: <201108032210.p73MARgD018596@mailhost2.mfldclin.edu>

We moved into a new lab a few years ago, and due to the incoming air current with the ventilation here, it caused our cyrostats to ice up considerably. We have the type of ventilation that has air vents on the ceiling and are constantly blowing large amounts of air into our rooms. We had to get a piece of plastic put into the vent to divert some of the air away from our cryostat. It helped, but still ices more than when we were in our old lab. Anyway, point is to check out the air flow above the cyrostat. You also need to have enough clearance behind the cryostat and on each side. The clearance amount would be listed in the manual. 
good luck
amysue

______________________________________________________________________
The contents of this message may contain private, protected and/or privileged information.  If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.  Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.  Thank you for your cooperation.
From amosbrooks <@t> gmail.com  Wed Aug  3 17:45:08 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Wed Aug  3 17:45:14 2011
Subject: [Histonet] Immuno. confusion
Message-ID: 

Hi,
    Well... almost any point before DAB. You wouldn't want to put it in
after the HRP conjugated secondary because that would quench the HRP signal
you are actually looking for leaving you nothing for the DAB to precipitate
on. On the other hand you certainly do not *need* to do it after the HIER as
it will work just fine before it or even after the primary antibody
(assuming it isn't HRP conjugated). Actually we have had the best results on
CD4 if the H2O2 is done prior to the HIER. The H2O2 in this case actually
affects the epitope if you do it after HIER. If it does that with CD4, I
assume it is likely to do so with other finicky epitopes.

Have a nice day,
Amos

On Wed, Aug 3, 2011 at 1:00 PM,
wrote:

> Message: 7
> Date: Wed, 3 Aug 2011 11:49:40 -0500
> From: "Troutman, Kenneth A" 
> Subject: [Histonet] Immuno. confusion
> To: "Histonet@lists.utsouthwestern.edu"
>        
> Message-ID:
>        <
> 7B310892042DA74CB3590053F424CFE6143EE301B7@ITS-HCWNEM06.ds.Vanderbilt.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Valerie,
>
> The peroxidase blocking step is to prevent endogenous peroxidase activity
> that will cause a false positive stemming from your application of DAB (the
> substrate for your DAB solution is hydrogen peroxide).  You can essentially
> put this blocking step at any point in your stain (following
> deparaffinization) and before your application of DAB.  I have run this step
> at varying points in my procedures and they all work fine.  Some antibodies
> prefer you do this step before primary application and others prior to DAB,
> but for most stains, it doesn't matter too much.
>
> As for an enhancer, you can make a copper sulfate solution (5mg/mL in
> buffer).  Or you can try DAB Enhancer or DAB Sparkle from Biocare.
>
> Good luck,
>
> Ashley Troutman BS, HT(ASCP) QIHC
> Immunohistochemistry Supervisor
> Vanderbilt University Histopathology
> 1301 Medical Center Drive TVC 4531
> Nashville, TN  37232
>
From abright <@t> brightinstruments.com  Thu Aug  4 07:38:35 2011
From: abright <@t> brightinstruments.com (abright@brightinstruments.com)
Date: Thu Aug  4 07:38:47 2011
Subject: [Histonet] microtome service/repair Denver area
In-Reply-To: <14E2C6176416974295479C64A11CB9AE1DECBA06B8@SBS2K8.premierlab.local>
References: <14E2C6176416974295479C64A11CB9AE1DECBA06B8@SBS2K8.premierlab.local>
Message-ID: <1698950468-1312461517-cardhu_decombobulator_blackberry.rim.net-303753835-@b26.c2.bise7.blackberry>

Dear Liz,

Why don't you use Leica services?

Alan Bright 
Sent from my BlackBerry? wireless device

-----Original Message-----
From: Elizabeth Chlipala 
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Wed, 3 Aug 2011 15:55:39 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome service/repair Denver area

Hello everyone

I was wondering if anyone has any recommendations for a vendor that does yearly service and repair on Leica microtomes and cryostats.  We have been working with one vendor for years now and always have been happy with their service but they have become very expensive, any suggestions would be appreciated.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From JWeems <@t> sjha.org  Thu Aug  4 08:53:39 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Thu Aug  4 08:53:46 2011
Subject: [Histonet] microtome service/repair Denver area
In-Reply-To: <1698950468-1312461517-cardhu_decombobulator_blackberry.rim.net-303753835-@b26.c2.bise7.blackberry>
References: <14E2C6176416974295479C64A11CB9AE1DECBA06B8@SBS2K8.premierlab.local>
	<1698950468-1312461517-cardhu_decombobulator_blackberry.rim.net-303753835-@b26.c2.bise7.blackberry>
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082128C597@CHEXCMS10.one.ads.che.org>

She said they've become too expensive. We all must economize these days!  


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of abright@brightinstruments.com
Sent: Thursday, August 04, 2011 08:39
To: Elizabeth Chlipala; histonet-bounces@lists.utsouthwestern.edu; Histonet
Subject: Re: [Histonet] microtome service/repair Denver area

Dear Liz,

Why don't you use Leica services?

Alan Bright
Sent from my BlackBerry(r) wireless device

-----Original Message-----
From: Elizabeth Chlipala 
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Wed, 3 Aug 2011 15:55:39
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome service/repair Denver area

Hello everyone

I was wondering if anyone has any recommendations for a vendor that does yearly service and repair on Leica microtomes and cryostats.  We have been working with one vendor for years now and always have been happy with their service but they have become very expensive, any suggestions would be appreciated.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From Bryan.Watson <@t> neiurology.com  Thu Aug  4 09:34:49 2011
From: Bryan.Watson <@t> neiurology.com (Bryan Watson)
Date: Thu Aug  4 09:34:57 2011
Subject: [Histonet] ThinPrep 2000
Message-ID: <1C03BD76DC9BD14EA266F05C65D173B4430F32FC1C@NEIU-SBS.Niu.lan>

We are looking into using a ThinPrep 2000 instrument for our urine cytologies. A rep from Hologic told my manager these can also be used to do FISH on urines as well. I am wondering if anyone uses the ThinPrep 2000 for this purpose and if so, how well do you like it?
Thanks,
Bryan
From Ghazal.Khan <@t> uchospitals.edu  Thu Aug  4 10:22:08 2011
From: Ghazal.Khan <@t> uchospitals.edu (Ghazal.Khan@uchospitals.edu)
Date: Thu Aug  4 10:22:12 2011
Subject: [Histonet] ThinPrep 2000
In-Reply-To: <1C03BD76DC9BD14EA266F05C65D173B4430F32FC1C@NEIU-SBS.Niu.lan>
References: <1C03BD76DC9BD14EA266F05C65D173B4430F32FC1C@NEIU-SBS.Niu.lan>
Message-ID: <04D92BC5F8E8FD4089836F60E2983FF302A843D5@uchmbx04-hpk03s.UCHAD.uchospitals.edu>

I know of a lab where I work sometimes (on call) they use thin prep 2000
and do the fish studies on the sample. I think it's working for them.
The lab name is Uro partners.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan
Watson
Sent: Thursday, August 04, 2011 9:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ThinPrep 2000

We are looking into using a ThinPrep 2000 instrument for our urine
cytologies. A rep from Hologic told my manager these can also be used to
do FISH on urines as well. I am wondering if anyone uses the ThinPrep
2000 for this purpose and if so, how well do you like it?
Thanks,
Bryan
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

********************************************************************************
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it is addressed and may contain information that is privileged and confidential.
If the reader of this e-mail message is not the intended recipient, you are 
hereby notified that any dissemination, distribution or copying of this
communication is prohibited. If you have received this e-mail in error, please 
notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 
********************************************************************************

From lbustamante <@t> cvm.tamu.edu  Thu Aug  4 10:56:58 2011
From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante)
Date: Thu Aug  4 10:57:11 2011
Subject: [Histonet] Texas Society Officer
Message-ID: <4E3A7AFA020000B90010D6FB@CVM.TAMU.EDU>

Please, I need to contact the Texas Society Treasurer or anyone that can
help me with a check I received from TSH by mistake.
Thank you.
Lin Bustamante

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas A&M University
College Station, TX 77843-4458
From Ghazal.Khan <@t> uchospitals.edu  Thu Aug  4 11:23:53 2011
From: Ghazal.Khan <@t> uchospitals.edu (Ghazal.Khan@uchospitals.edu)
Date: Thu Aug  4 11:23:58 2011
Subject: [Histonet] Job opening
In-Reply-To: <4E3A7AFA020000B90010D6FB@CVM.TAMU.EDU>
References: <4E3A7AFA020000B90010D6FB@CVM.TAMU.EDU>
Message-ID: <04D92BC5F8E8FD4089836F60E2983FF302A843D6@uchmbx04-hpk03s.UCHAD.uchospitals.edu>


I know this position for a full time histotech at Palos Community
hospital. Palos heights, Il

Please contact Linda Coomer at 708-923-5094.  You can give her my
reference

Thanks 
ghazal

********************************************************************************
This e-mail is intended only for the use of the individual or entity to which
it is addressed and may contain information that is privileged and confidential.
If the reader of this e-mail message is not the intended recipient, you are 
hereby notified that any dissemination, distribution or copying of this
communication is prohibited. If you have received this e-mail in error, please 
notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 
********************************************************************************

From hlukey <@t> msn.com  Thu Aug  4 13:52:17 2011
From: hlukey <@t> msn.com (Hugh Luk)
Date: Thu Aug  4 13:52:21 2011
Subject: [Histonet] Manual embedding
In-Reply-To: 
References: 
Message-ID: 










Scott,

Pacific Southwest Lab Equipment in California has been good to me.  Very fair prices compared to new.  However, I also must state that two of the four items purchased "Used" from them no longer work (embedder, cryostat) BECAUSE of the new freon regulations.  Be aware that in older embedders, if the refrigerant gives out, it cannot be serviced legally, as the old freon was outlawed.  To replace the coolant, the whole refrigeration system will have to be rebuilt (evaporator, compressor, ect).

Sorry to state this to you, but the embedder that save my sanity, was purchased new.

I agree with Stella's email, as the nicest way to "Get" an embedder or paraffin pot is to find someone who does not need it anymore (like a local hospital or clinic that could donate to your university).  When I first started research, and we had no money for equipment, I purchased a paraffin foot-bath, like the HoMedics one at Target.  It worked okay, but it was cumbersome.  And where was that lavender-smell coming from?

Also, I got a great Leica electric paraffin pitcher from eBay for like $80 and a forceps warmer for $35.

Good luck with your search,
Hugh
Also in University research
Hawaii


> Date: Tue, 2 Aug 2011 22:53:38 -0400
> From: sparker@vt.edu
> To: Histonet@lists.utsouthwestern.edu
> CC: 
> Subject: [Histonet] Manual embedding
> 
> Dear Histonetters:
> 
> I am interested in acquiring a pitcher and heating jacket for melting and
> pouring paraffin during manual embedding. My work is relatively low volume
> and in a university research lab setting so I am trying to avoid purchasing
> an expensive embedding station. Can anyone recommend an honest supplier of
> used histology equipment that might be able to provide me with this item?
> 
> Thank you for your expertise!
> 
> Scott L. Parker
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


 		 	   		  
From thisisann <@t> aol.com  Thu Aug  4 14:11:38 2011
From: thisisann <@t> aol.com (Ann Angelo)
Date: Thu Aug  4 14:11:57 2011
Subject: [Histonet] Alternate embedding media
Message-ID: <8CE20F1782C7B05-DA4-16A74@webmail-d079.sysops.aol.com>


Can anyone tell me if they know of an alternative embedding media to paraffin?  Does "SAEF" mean anything to anyone?
Thanks,  Ann



From jp1000r <@t> hotmail.com  Thu Aug  4 14:27:22 2011
From: jp1000r <@t> hotmail.com (Jean-Philippe REY)
Date: Thu Aug  4 14:27:26 2011
Subject: [Histonet] Certification
Message-ID: 


I intend to take the HT/HTL certification before summer 2012 and would like to team up with people with the same interest to create a work group to prepare for that test.
The group will gather once or twice a week for an hour, or so, to go over a partial or entire Chapter of the Histotechnology: Self-Instructional text book from F. Carson.
If you are living in the area of Kansas City (MO or KS) and are interested into it please contact me.
 
Jean-Philippe Rey
(816) 213-2558
jp1000r@hotmail.com 		 	   		  
From MLafrini <@t> csmlab.com  Thu Aug  4 15:11:03 2011
From: MLafrini <@t> csmlab.com (Michael LaFriniere)
Date: Thu Aug  4 15:11:24 2011
Subject: [Histonet] Job opening
In-Reply-To: <04D92BC5F8E8FD4089836F60E2983FF302A843D6@uchmbx04-hpk03s.UCHAD.uchospitals.edu>
References: <4E3A7AFA020000B90010D6FB@CVM.TAMU.EDU>
	<04D92BC5F8E8FD4089836F60E2983FF302A843D6@uchmbx04-hpk03s.UCHAD.uchospitals.edu>
Message-ID: <4E3AC4A1.588C.00AF.0@csmlab.com>

I have a few PT histotech positions openings...1 in Reston Virginia and 1 in Laurel Maryland. Please feel free to contact me if any interest.

 
 
Michael
 
Michael R. LaFriniere, HT (ASCP) 
Executive Director
Cytology Services of Maryland (CSM)
13900 Laurel Lakes Ave., Suite 100 
Laurel, MD 20707
301-206-2555 ext 27    
410-940-8844 cell
301-206-2595 fax
michael.lafriniere@csmlab.com

From brod033 <@t> gmail.com  Thu Aug  4 15:39:21 2011
From: brod033 <@t> gmail.com (Ben Spirto)
Date: Thu Aug  4 15:39:24 2011
Subject: [Histonet] Looking for a position in Chicago land area
Message-ID: 

Hi all,

I am looking for a Histotech position in Chicago and suburbs. I have
experience in immunohistochemistry (manual and automated), sectioning (4, 6,
20, 40 microns) and embedding.
I have 4 years of experience and BS in Biology. I am HTL/HT as well as QIHC
eligible.

For more info please contact me at: brod033@gmail.com

Thanks
From POWELL_SA <@t> mercer.edu  Thu Aug  4 16:13:49 2011
From: POWELL_SA <@t> mercer.edu (Shirley A. Powell)
Date: Thu Aug  4 16:13:58 2011
Subject: [Histonet] histology equipment service
Message-ID: <9BF995BC0E47744E9673A41486E24EE238DCB37FC2@MERCERMAIL.MercerU.local>

For the person/persons looking for someone to do equipment maintenance/service I can highly recommend this company, Pathology Service, Inc.  Sean Draime and Joel DeVisser are very knowledgeable on all types of histology equipment and their charges are reasonable.  They are in Georgia, of course, GO DAWGS, but travel all over the country doing repair, PM, and also have used equipment now.  Their number is 1-866-398-9478, or contact them by going to www.pathologyserv.com.  Also see email below for more information on equipment purchases.


To: Shirley A. Powell
Subject: Joel from PSI

Hi, this is my email here for any info you come across reference people who may need to sell any lab equipment or labs.  We also do offer very competitive service and preventative maintenance packages and cover most of the USA.  Sean has 3 different plans that can be customized to fit any clients needs or budgets.  We also sell used, refurbished equipment and our pieces go through frame off restorations and come out working and looking like new again.  We offer 90 day warranties on everything we sell and 1 year extensions all through PSI.  Thank you so much for the information on the news letter and for taking the time to speak with me earlier on the phone.

Joel DeVisser
678-887-6068
From POWELL_SA <@t> mercer.edu  Thu Aug  4 16:25:44 2011
From: POWELL_SA <@t> mercer.edu (Shirley A. Powell)
Date: Thu Aug  4 16:25:57 2011
Subject: [Histonet] FW: histology equipment service
Message-ID: <9BF995BC0E47744E9673A41486E24EE238DCB37FE5@MERCERMAIL.MercerU.local>

Okay the website is www.pathologyserv.com for some reason it was duplicated.  On the site it focuses on eastern states but they have expanded to cover most of the USA.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell
Sent: Thursday, August 04, 2011 5:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] histology equipment service

For the person/persons looking for someone to do equipment maintenance/service I can highly recommend this company, Pathology Service, Inc.  Sean Draime and Joel DeVisser are very knowledgeable on all types of histology equipment and their charges are reasonable.  They are in Georgia, of course, GO DAWGS, but travel all over the country doing repair, PM, and also have used equipment now.  Their number is 1-866-398-9478, or contact them by going to www.pathologyserv.com.  Also see email below for more information on equipment purchases.


To: Shirley A. Powell
Subject: Joel from PSI

Hi, this is my email here for any info you come across reference people who may need to sell any lab equipment or labs.  We also do offer very competitive service and preventative maintenance packages and cover most of the USA.  Sean has 3 different plans that can be customized to fit any clients needs or budgets.  We also sell used, refurbished equipment and our pieces go through frame off restorations and come out working and looking like new again.  We offer 90 day warranties on everything we sell and 1 year extensions all through PSI.  Thank you so much for the information on the news letter and for taking the time to speak with me earlier on the phone.

Joel DeVisser
678-887-6068
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From amitapandey <@t> torrentpharma.com  Fri Aug  5 02:24:31 2011
From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com)
Date: Fri Aug  5 02:26:04 2011
Subject: [Histonet] your opinion on microtome
In-Reply-To: 
References: 
Message-ID: 

Hello friends,

I am planning to  buy a microtome for my lab ....i already have Leica 
manual one which is working perfectly.

Now I wish to introduce a fully motorized one. 

Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME.

Let me know your frank review on
1. manual versus automated option
2. Above all three items.in terms of their performance, maintenance and 
breakdown etc. or due you suggest any more better option


Thanks in advance for your feed back. In past also histonetters helped me 
to solve many problems, looking forward this time also.

Amita
From relia1 <@t> earthlink.net  Fri Aug  5 08:26:07 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Fri Aug  5 08:26:09 2011
Subject: [Histonet] RELIA Hot Histology Job Alert 3 month Assignment in
	Miami with one of my best clients.
Message-ID: <5A1EBC8BB79242C2831D31E4E335EAD4@ownerf1abaad51>

Hi Histonetters,
I hope you are having a great day!!  I just received a call from one of
my best clients located in Miami, FL. While I work exclusively in
permanent placement they have asked for my assistance in finding a
Florida licensed histotechnician for a 3 month assignment.  The shift is
4a-1230p.  They prefer a local candidate and derm experience is a plus.
Here is the KICKER... If you are a new graduate of a histology program
who has a Florida license my client wants to talk to you too!!!  If you
or anyone you know might be interested in this opportunity please shoot
me an e-mail at relia1@earthlink.net or give me a call on my cell phone
at 407-353-5070.  
 

Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.comPamBarkerRELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From dliaros <@t> BioReference.com  Fri Aug  5 09:47:40 2011
From: dliaros <@t> BioReference.com (Donna Liaros)
Date: Fri Aug  5 09:48:01 2011
Subject: [Histonet] Arg-1
Message-ID: <6CB019913E5F6940AD6E4871841EC5364AD8CE@MBX-2.biosvr1.bioreference.com>

I have to work up the antibody ARG-1 and would like to get some feedback on suggested protocols.  I have the concentrated antibody from ABCAM.

Thanks,
Donna
The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof.
From TGoins <@t> mt.gov  Fri Aug  5 09:57:57 2011
From: TGoins <@t> mt.gov (Goins, Tresa)
Date: Fri Aug  5 09:58:36 2011
Subject: [Histonet] your opinion on microtome
In-Reply-To: 
References: 
	
Message-ID: 

We have a Reichert-Jung 820 manual (old old old) and a fully automated Microm HM 355.  Both of us who cut prefer the manual.

The manual is more compact, easier to clean, cheaper. 
The Microm HM 355 is twice as big and the blade holder - adjustment screw set-up is neither convenient or easy to optimize.

Bottom line, the fewer moving parts the better.

Tresa     


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amitapandey@torrentpharma.com
Sent: Friday, August 05, 2011 1:25 AM
To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu
Subject: [Histonet] your opinion on microtome

Hello friends,

I am planning to  buy a microtome for my lab ....i already have Leica 
manual one which is working perfectly.

Now I wish to introduce a fully motorized one. 

Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME.

Let me know your frank review on
1. manual versus automated option
2. Above all three items.in terms of their performance, maintenance and 
breakdown etc. or due you suggest any more better option


Thanks in advance for your feed back. In past also histonetters helped me 
to solve many problems, looking forward this time also.

Amita
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From rjbuesa <@t> yahoo.com  Fri Aug  5 10:03:26 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Aug  5 10:03:30 2011
Subject: [Histonet] your opinion on microtome
In-Reply-To: 
Message-ID: <1312556606.81347.YahooMailClassic@web65716.mail.ac4.yahoo.com>

The same (the less moving parts the better) applies to any instrument or piece of equipment.
Remember the VW Beetle?
Ren? J.

--- On Fri, 8/5/11, Goins, Tresa  wrote:


From: Goins, Tresa 
Subject: RE: [Histonet] your opinion on microtome
To: "amitapandey@torrentpharma.com" , "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" 
Date: Friday, August 5, 2011, 10:57 AM


We have a Reichert-Jung 820 manual (old old old) and a fully automated Microm HM 355.? Both of us who cut prefer the manual.

The manual is more compact, easier to clean, cheaper. 
The Microm HM 355 is twice as big and the blade holder - adjustment screw set-up is neither convenient or easy to optimize.

Bottom line, the fewer moving parts the better.

Tresa? ???


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amitapandey@torrentpharma.com
Sent: Friday, August 05, 2011 1:25 AM
To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu
Subject: [Histonet] your opinion on microtome

Hello friends,

I am planning to? buy a microtome for my lab ....i already have Leica 
manual one which is working perfectly.

Now I wish to introduce a fully motorized one. 

Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME.

Let me know your frank review on
1. manual versus automated option
2. Above all three items.in terms of their performance, maintenance and 
breakdown etc. or due you suggest any more better option


Thanks in advance for your feed back. In past also histonetters helped me 
to solve many problems, looking forward this time also.

Amita
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From PAMarcum <@t> uams.edu  Fri Aug  5 10:13:46 2011
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Fri Aug  5 10:13:53 2011
Subject: [Histonet] your opinion on microtome
In-Reply-To: 
References: 
	
	
Message-ID: 

We have 3 Leica RM2155s, 3 RM2255 and one Microm 355S.  We all love the Leica models and some prefer one or the other. I do find I am the only one to use the foot petal or most of the really automated features.  The Microm is a good microtome however; only person uses it daily and no one else seems to want to switch.  I have used the Thermo Millennium and it is a good microtome too.  I loved the old black AO I started on and still miss sometimes.  We have a Reichert Jung 2030 Rotary that also works very well.  I think it is what you are accustomed to using and the motorized microtomes while good are expensive and it you don't need or want the features it can be a waste of money. 

I do love the using the automated fine/coarse advance on the motorized as opposed to turning a wheel.  It is easier on the hands and wrists for repetitive motion injuries.  

Pam Marcum
UAMS
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Friday, August 05, 2011 9:58 AM
To: amitapandey@torrentpharma.com; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu
Subject: RE: [Histonet] your opinion on microtome

We have a Reichert-Jung 820 manual (old old old) and a fully automated Microm HM 355.  Both of us who cut prefer the manual.

The manual is more compact, easier to clean, cheaper. 
The Microm HM 355 is twice as big and the blade holder - adjustment screw set-up is neither convenient or easy to optimize.

Bottom line, the fewer moving parts the better.

Tresa     


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amitapandey@torrentpharma.com
Sent: Friday, August 05, 2011 1:25 AM
To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu
Subject: [Histonet] your opinion on microtome

Hello friends,

I am planning to  buy a microtome for my lab ....i already have Leica 
manual one which is working perfectly.

Now I wish to introduce a fully motorized one. 

Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME.

Let me know your frank review on
1. manual versus automated option
2. Above all three items.in terms of their performance, maintenance and 
breakdown etc. or due you suggest any more better option


Thanks in advance for your feed back. In past also histonetters helped me 
to solve many problems, looking forward this time also.

Amita
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information.  Any unauthorized review,
use, disclosure or distribution is prohibited.  If you are not the 
intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message..


From Nacaela.Johnson <@t> USONCOLOGY.COM  Fri Aug  5 10:31:30 2011
From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela)
Date: Fri Aug  5 10:32:46 2011
Subject: [Histonet] Kappa/Lambda ISH background
Message-ID: <71882EED22A283429E8424513A22922D595366@txhous1eb015.uson.usoncology.int>

Would anyone be willing to share their protocols for the Kappa and
Lambda ISH?  I cannot get rid of the eosinophilic background staining.
 

Thanks,

 

Nacaela Johnson, B.S. HTL (ASCP)CM

Histotechnologist

KCCC Pathology

12000 110th St., Ste. 400

Overland Park, KS 66210

Office:  913-234-0576

Fax:  913-433-7639

Email:  Nacaela.Johnson@USOncology.com
The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From alyssa <@t> alliedsearchpartners.com Fri Aug 5 10:53:38 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Aug 5 10:53:45 2011 Subject: [Histonet] Tech Needed For Vaca Coverage Next Week! Message-ID: Hello, I'm in need of a Histotech for a laboratory in Hadden Hieghts, NJ which is about 10 miles outside of Philadelphia. It would be next week, August 16th (Tuesday) through August 19th (Friday). Please let me know if you or anyone you might know could help me out! :-) Thanks! -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From marktarango <@t> gmail.com Fri Aug 5 10:54:07 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Aug 5 10:54:11 2011 Subject: [Histonet] Kappa/Lambda ISH background In-Reply-To: <71882EED22A283429E8424513A22922D595366@txhous1eb015.uson.usoncology.int> References: <71882EED22A283429E8424513A22922D595366@txhous1eb015.uson.usoncology.int> Message-ID: How are you doing your ISH? A commercial manual kit, home-made kit, instrument? On Fri, Aug 5, 2011 at 8:31 AM, Johnson, Nacaela < Nacaela.Johnson@usoncology.com> wrote: > Would anyone be willing to share their protocols for the Kappa and > Lambda ISH? I cannot get rid of the eosinophilic background staining. > > > Thanks, > > > > Nacaela Johnson, B.S. HTL (ASCP)CM > > Histotechnologist > > KCCC Pathology > > 12000 110th St., Ste. 400 > > Overland Park, KS 66210 > > Office: 913-234-0576 > > Fax: 913-433-7639 > > Email: Nacaela.Johnson@USOncology.com > > > The contents of this electronic mail message and any attachments are > confidential, possibly privileged and intended for the addressee(s) > only.
Only the addressee(s) may read, disseminate, retain or otherwise > use this message. If received in error, please immediately inform the sender > and then delete this message without disclosing its contents to > anyone. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BDeBrosse-Serra <@t> isisph.com Fri Aug 5 12:20:44 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Aug 5 12:20:59 2011 Subject: [Histonet] your opinion on microtome In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F08FE8409FF@EXCHMB01.isis.local> My favorite is the Leica RM2255. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, August 05, 2011 7:58 AM To: amitapandey@torrentpharma.com; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] your opinion on microtome We have a Reichert-Jung 820 manual (old old old) and a fully automated Microm HM 355. Both of us who cut prefer the manual. The manual is more compact, easier to clean, cheaper. The Microm HM 355 is twice as big and the blade holder - adjustment screw set-up is neither convenient or easy to optimize. Bottom line, the fewer moving parts the better. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amitapandey@torrentpharma.com Sent: Friday, August 05, 2011 1:25 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] your opinion on microtome Hello friends, I am planning to buy a microtome for my lab ....i already have Leica manual one which is working perfectly. Now I wish to introduce a fully motorized one. Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME. Let me know your frank review on 1. manual versus automated option 2. Above all three items.in terms of their performance, maintenance and breakdown etc. or due you suggest any more better option Thanks in advance for your feed back. In past also histonetters helped me to solve many problems, looking forward this time also. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nacaela.Johnson <@t> USONCOLOGY.COM Fri Aug 5 13:48:44 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Fri Aug 5 13:49:48 2011 Subject: [Histonet] Kappa/Lambda ISH background In-Reply-To: References: <71882EED22A283429E8424513A22922D595366@txhous1eb015.uson.usoncology.int> Message-ID: <71882EED22A283429E8424513A22922D595368@txhous1eb015.uson.usoncology.int> I am performing the ISH on the Leica Bond. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com ________________________________ From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Friday, August 05, 2011 10:54 AM To: Johnson, Nacaela Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Kappa/Lambda ISH background How are you doing your ISH? A commercial manual kit, home-made kit, instrument? On Fri, Aug 5, 2011 at 8:31 AM, Johnson, Nacaela wrote: Would anyone be willing to share their protocols for the Kappa and Lambda ISH? I cannot get rid of the eosinophilic background staining. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From jimr0712 <@t> comcast.net Sun Aug 7 04:33:11 2011 From: jimr0712 <@t> comcast.net (jimr0712@comcast.net) Date: Sun Aug 7 04:33:21 2011 Subject: [Histonet] (no subject) Message-ID: <59590761.281178.1312709591856.JavaMail.root@sz0098a.emeryville.ca.mail.comcast.net> .. http://www.badantecercasi.it/dkiurytq1.htm?ushowtopic=y2ziy From rgeske_2000 <@t> yahoo.com Sun Aug 7 12:38:58 2011 From: rgeske_2000 <@t> yahoo.com (Rob Geske) Date: Sun Aug 7 12:39:02 2011 Subject: [Histonet] 20 slide holder for Microprobe system Message-ID: <1312738738.68756.YahooMailNeo@web39410.mail.mud.yahoo.com> All, ? i'm looking for a supplier of the 20 slide holder/handle for the microprobe staining system previously sold by fisher.?fisher has discontinue this as too has cole palmer.? the entire system is available on e-bay and also through some used equipment re-sellers, but i just?need the handle.? any help would be appreciated. ? thanks in advance, rob From Karen.Heckford <@t> CHW.edu Mon Aug 8 07:10:57 2011 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Mon Aug 8 07:11:04 2011 Subject: [Histonet] problems posting Message-ID: <9ECF174E7DA83046BE6EBFDE009E28A388C413@CHW-MSG-301.chw.edu> I am having problems posting to Histonet. Not sure what is going on. Hopefully this goes through if not please add me to your list. I have been on the the Histonet for 7 years now. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 616 7Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From Karen.Heckford <@t> CHW.edu Mon Aug 8 07:19:24 2011 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Mon Aug 8 07:19:30 2011 Subject: [Histonet] Per diem Position in the San Francisco Bay Area Message-ID: <9ECF174E7DA83046BE6EBFDE009E28A388C414@CHW-MSG-301.chw.edu> We are looking for a Certified Histology Technician for per diem work. You will also do some Pathology Assisting (nothing to extensive on the PA). Need someone that can cover vacation, sick days, etc. Perfect for someone that is retired and is looking for some extra money. Need to be able to work on your own independently and know how to troubleshoot both Histology and IHC's. You will need to be able to be available to work up to 1-2 weeks at a time or get called in at the last minute in case someone is sick. So a flexible schedule is ideal. If interested please call and leave me a message. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 616 7Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From Carol.Fields <@t> Northside.com Mon Aug 8 07:48:20 2011 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Mon Aug 8 07:48:34 2011 Subject: [Histonet] histology equipment service In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238DCB37FC2@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE238DCB37FC2@MERCERMAIL.MercerU.local> Message-ID: <731941C266951A47BEF11E5EFAAED9C90B666B7C@nsmvexch01.northside.local> Southeast Pathology Services, Inc. we have used for years and do an excellent job. They also have refurbed equipment. Call Michael Dietrich at 843-588-2559 Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, August 04, 2011 5:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histology equipment service For the person/persons looking for someone to do equipment maintenance/service I can highly recommend this company, Pathology Service, Inc. Sean Draime and Joel DeVisser are very knowledgeable on all types of histology equipment and their charges are reasonable. They are in Georgia, of course, GO DAWGS, but travel all over the country doing repair, PM, and also have used equipment now. Their number is 1-866-398-9478, or contact them by going to www.pathologyserv.com. Also see email below for more information on equipment purchases. To: Shirley A. Powell Subject: Joel from PSI Hi, this is my email here for any info you come across reference people who may need to sell any lab equipment or labs. We also do offer very competitive service and preventative maintenance packages and cover most of the USA. Sean has 3 different plans that can be customized to fit any clients needs or budgets. We also sell used, refurbished equipment and our pieces go through frame off restorations and come out working and looking like new again. We offer 90 day warranties on everything we sell and 1 year extensions all through PSI. Thank you so much for the information on the news letter and for taking the time to speak with me earlier on the phone. Joel DeVisser 678-887-6068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From srishan <@t> mail.holyname.org Mon Aug 8 08:28:33 2011 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Mon Aug 8 08:28:55 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks Message-ID: Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From sbreeden <@t> nmda.nmsu.edu Mon Aug 8 08:46:50 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Aug 8 08:46:54 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: References: Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF967@nmdamailsvr.nmda.ad.nmsu.edu> AHA! I have an Answer! EMS (Electron Microscopy Sciences) sells a "One-Piece Scalpel Blade with Handle" Cat. #72048-01 that is perfect for cleaning blocks of excess paraffin! I've had one for over 30 years and it will retire with me! It is initially sharp (but not as sharp as a normal blade would be) but if you cut open a few cardboard boxes with it (which it also does beautifully), it "dulls down" to the perfect edge for cleaning blocks. It fits the hand well and doesn't shave the plastic off the cassette. Someone left an old paring knife here but it's too big and cumbersome. From rjbuesa <@t> yahoo.com Mon Aug 8 08:58:47 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 8 08:58:52 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: Message-ID: <1312811927.72000.YahooMailClassic@web65713.mail.ac4.yahoo.com> I always used my Wenger Swiss Army pocket knife. Ren? J. --- On Mon, 8/8/11, srishan@mail.holyname.org wrote: From: srishan@mail.holyname.org Subject: [Histonet] knife used for cleaning paraffin off the blocks To: histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu Date: Monday, August 8, 2011, 9:28 AM Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks.? I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Aug 8 09:32:08 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Aug 8 09:32:16 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org> We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Marcia_Gaiser <@t> ssmhc.com Mon Aug 8 09:33:43 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Mon Aug 8 09:33:51 2011 Subject: [Histonet] re-cycled xylene in tissue processor Message-ID: <728F817C02110E498D803A7C3B0C6248068D43B5E0@S009-APEXM06.ds.ad.ssmhc.com> Hi, Has anyone had experience using re-cycled xylene in the Tissue-Tek VIP5 tissue processor for the clean cycle? Will use of re-cycled xylene, over time, damage the processor? Thank you, Marcia Gaiser Pathology Supervisor Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Mon Aug 8 09:45:08 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 8 09:45:13 2011 Subject: [Histonet] re-cycled xylene in tissue processor In-Reply-To: <728F817C02110E498D803A7C3B0C6248068D43B5E0@S009-APEXM06.ds.ad.ssmhc.com> Message-ID: <1312814708.22223.YahooMailClassic@web65710.mail.ac4.yahoo.com> If you xylene recycler works according to standard and maintained properly, the recycled xylene you obtain is of equal quality to the xylene you buy from your usual supplier. It can be used in any and all steps of your processing and staining protocols, and there is no way it could "damage" your or any other tissue processing using xylene. All the "difficulties" with recycled xylene are caused by improper recycling. Ren? J. --- On Mon, 8/8/11, Gaiser, Marcia wrote: From: Gaiser, Marcia Subject: [Histonet] re-cycled xylene in tissue processor To: "histonet@lists.utsouthwestern.edu" Date: Monday, August 8, 2011, 10:33 AM Hi, Has anyone had experience using re-cycled xylene in the Tissue-Tek VIP5 tissue processor for the clean cycle? Will use of re-cycled xylene, over time, damage the processor? Thank you, Marcia Gaiser Pathology Supervisor Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Aug 8 09:46:31 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Aug 8 09:46:35 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org> Message-ID: <1312814791.72524.YahooMailRC@web1113.biz.mail.sk1.yahoo.com> I just use the handle end of my forceps. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Horn, Hazel V" To: "srishan@mail.holyname.org" ; "histonet-bounces@lists.utsouthwestern.edu" ; "histonet@lists.utsouthwestern.edu" Sent: Mon, August 8, 2011 9:32:08 AM Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way? ? Slot 820 Little Rock, AR? 72202 phone? 501.364.4240 fax? ? ? ? 501.364.3155 visit us on the web at:? ? www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks.? I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Mon Aug 8 09:50:25 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Aug 8 09:50:45 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: <1312814791.72524.YahooMailRC@web1113.biz.mail.sk1.yahoo.com> References: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org> <1312814791.72524.YahooMailRC@web1113.biz.mail.sk1.yahoo.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F509F0@SMCMAIL01.somerset-healthcare.com> I usually use one of the embedding molds. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 08, 2011 10:47 AM To: Histonet Subject: [Histonet] knife used for cleaning paraffin off the blocks I just use the handle end of my forceps. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Horn, Hazel V" To: "srishan@mail.holyname.org" ; "histonet-bounces@lists.utsouthwestern.edu" ; "histonet@lists.utsouthwestern.edu" Sent: Mon, August 8, 2011 9:32:08 AM Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way? ? Slot 820 Little Rock, AR? 72202 phone? 501.364.4240 fax? ? ? ? 501.364.3155 visit us on the web at:? ? www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks.? I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From sfonner <@t> labpath.com Mon Aug 8 09:47:54 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Mon Aug 8 09:51:59 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org> Message-ID: <003f01cc55da$27917f60$76b47e20$@com> Since I haven't seen anyone talk about this yet, I feel obligated to tell you all that there is a handy dandy little "melter" out there for getting the excess paraffin off the blocks. You just run the edges of the block over it and voila...trimmed. When you have up to 600 blocks a day, this thing is wonderful. And it keeps your hands from cramping up. Oh the wonders!! Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, August 08, 2011 10:32 AM To: 'srishan@mail.holyname.org'; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Aug 8 09:52:21 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Mon Aug 8 09:52:28 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F509F0@SMCMAIL01.somerset-healthcare.com> References: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org> <1312814791.72524.YahooMailRC@web1113.biz.mail.sk1.yahoo.com> <3AD061FE740D464FAC7BF6B5CFB7570711F509F0@SMCMAIL01.somerset-healthcare.com> Message-ID: I don't have much paraffin on the edges of the block but if I do I pop it off with the edge of my finger nail...... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Monday, August 08, 2011 10:50 AM To: 'Paula Pierce'; Histonet Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks I usually use one of the embedding molds. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 08, 2011 10:47 AM To: Histonet Subject: [Histonet] knife used for cleaning paraffin off the blocks I just use the handle end of my forceps. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Horn, Hazel V" To: "srishan@mail.holyname.org" ; "histonet-bounces@lists.utsouthwestern.edu" ; "histonet@lists.utsouthwestern.edu" Sent: Mon, August 8, 2011 9:32:08 AM Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way? ? Slot 820 Little Rock, AR? 72202 phone? 501.364.4240 fax? ? ? ? 501.364.3155 visit us on the web at:? ? www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks.? I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From laurie.colbert <@t> huntingtonhospital.com Mon Aug 8 09:53:42 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Aug 8 09:53:45 2011 Subject: [Histonet] re-cycled xylene in tissue processor In-Reply-To: <728F817C02110E498D803A7C3B0C6248068D43B5E0@S009-APEXM06.ds.ad.ssmhc.com> References: <728F817C02110E498D803A7C3B0C6248068D43B5E0@S009-APEXM06.ds.ad.ssmhc.com> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AF7D@EXCHANGE3.huntingtonhospital.com> We use recycled xylene for the cleaning cycle on our VIP 5's and have had no problems. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gaiser, Marcia Sent: Monday, August 08, 2011 7:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re-cycled xylene in tissue processor Hi, Has anyone had experience using re-cycled xylene in the Tissue-Tek VIP5 tissue processor for the clean cycle? Will use of re-cycled xylene, over time, damage the processor? Thank you, Marcia Gaiser Pathology Supervisor Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From je2 <@t> sanger.ac.uk Mon Aug 8 09:55:00 2011 From: je2 <@t> sanger.ac.uk (Jeanne Estabel) Date: Mon Aug 8 09:55:13 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks References: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org> <003f01cc55da$27917f60$76b47e20$@com> Message-ID: <34627BB49F43DC43A4A7D90B1B6F739D0115E1AB@exchsrv4.internal.sanger.ac.uk> Hi, Here we are using the "melter" or the knife depends on the technician and the number of blocks. Regards Jeanne Jeanne Estabel, PhD Scientific Manager Histology Operations Manager Mouse Genetics Project Wellcome Trust Sanger Institute Cambridge, UK Tel:+44 (0)1223 834244 ext 8306 Find Sanger Mouse Genetics Project phenotyping data on http://www.sanger.ac.uk/mouseportal/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: 08 August 2011 15:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks Since I haven't seen anyone talk about this yet, I feel obligated to tell you all that there is a handy dandy little "melter" out there for getting the excess paraffin off the blocks. You just run the edges of the block over it and voila...trimmed. When you have up to 600 blocks a day, this thing is wonderful. And it keeps your hands from cramping up. Oh the wonders!! Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, August 08, 2011 10:32 AM To: 'srishan@mail.holyname.org'; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE. From gayle.callis <@t> bresnan.net Mon Aug 8 09:56:25 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Aug 8 09:56:44 2011 Subject: [Histonet] Re: Knife for trimming paraffin from blocks Message-ID: <000001cc55db$59f88600$0de99200$@bresnan.net> You Wrote: I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. ************************************************************************* Dear Mala, In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer with a heated, slanted metal surface. This trimmer has made everyone happy by eliminating the potential for serious injury and does an even better job of getting rid of excess paraffin. There are two models sold, one from Thermo Scientific and the other one was recently spotted on a vendor website. (Sorry, I didn't jot the name down). The trimmers are worth the investment to keep you and your employees safe from nasty cuts, no matter what the knife/blade, etc could be used. The only drawback to the Para Trimmer is the messy paraffin drippings have to be collected in some separate container that the manufacturer doesn't supply. This is more than a bit annoying. It seems to me the manufacturer of these devices would design the trimmer with a paraffin catch tray. A small aluminum baking pan (from grocery store) could be used and disposed of (not a "Green" consideration). Be safe rather than sorry. Gayle M. Callis HTL/HT/MT(ASCP) From sdysart <@t> mirnarx.com Mon Aug 8 09:59:04 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Mon Aug 8 09:59:09 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: <34627BB49F43DC43A4A7D90B1B6F739D0115E1AB@exchsrv4.internal.sanger.ac.uk> References: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org><003f01cc55da$27917f60$76b47e20$@com> <34627BB49F43DC43A4A7D90B1B6F739D0115E1AB@exchsrv4.internal.sanger.ac.uk> Message-ID: The melters are pretty cool, but they have a pretty cool price tag too! If you are on a budget, this is not practical. I use a scalpel. Just make sure you dull the edge a bit before you use it so you don't chop your fingers off =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeanne Estabel Sent: Monday, August 08, 2011 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks Hi, Here we are using the "melter" or the knife depends on the technician and the number of blocks. Regards Jeanne Jeanne Estabel, PhD Scientific Manager Histology Operations Manager Mouse Genetics Project Wellcome Trust Sanger Institute Cambridge, UK Tel:+44 (0)1223 834244 ext 8306 Find Sanger Mouse Genetics Project phenotyping data on http://www.sanger.ac.uk/mouseportal/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: 08 August 2011 15:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks Since I haven't seen anyone talk about this yet, I feel obligated to tell you all that there is a handy dandy little "melter" out there for getting the excess paraffin off the blocks. You just run the edges of the block over it and voila...trimmed. When you have up to 600 blocks a day, this thing is wonderful. And it keeps your hands from cramping up. Oh the wonders!! Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, August 08, 2011 10:32 AM To: 'srishan@mail.holyname.org'; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfonner <@t> labpath.com Mon Aug 8 09:55:20 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Mon Aug 8 09:59:28 2011 Subject: [Histonet] Re: Knife for trimming paraffin from blocks In-Reply-To: <000001cc55db$59f88600$0de99200$@bresnan.net> References: <000001cc55db$59f88600$0de99200$@bresnan.net> Message-ID: <004c01cc55db$31f23f20$95d6bd60$@com> Gayle, We just use small disposable weigh boats to catch our drippings and toss them when full. Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, August 08, 2011 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Knife for trimming paraffin from blocks You Wrote: I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. ************************************************************************* Dear Mala, In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer with a heated, slanted metal surface. This trimmer has made everyone happy by eliminating the potential for serious injury and does an even better job of getting rid of excess paraffin. There are two models sold, one from Thermo Scientific and the other one was recently spotted on a vendor website. (Sorry, I didn't jot the name down). The trimmers are worth the investment to keep you and your employees safe from nasty cuts, no matter what the knife/blade, etc could be used. The only drawback to the Para Trimmer is the messy paraffin drippings have to be collected in some separate container that the manufacturer doesn't supply. This is more than a bit annoying. It seems to me the manufacturer of these devices would design the trimmer with a paraffin catch tray. A small aluminum baking pan (from grocery store) could be used and disposed of (not a "Green" consideration). Be safe rather than sorry. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathleen.Cormier <@t> crl.com Mon Aug 8 10:03:39 2011 From: Kathleen.Cormier <@t> crl.com (Cormier, Kathleen) Date: Mon Aug 8 10:03:44 2011 Subject: [Histonet] Re: Knife for trimming paraffin from blocks In-Reply-To: <000001cc55db$59f88600$0de99200$@bresnan.net> References: <000001cc55db$59f88600$0de99200$@bresnan.net> Message-ID: <895CFE180FF6704FBA7C0257D8DE344855D36D@ent-pr-xch-05.na01.crl.com> We use the paratrimmer too. What we do for a paraffin catcher is to use a specimen cup (I call them urine cups, but whatever) to catch the paraffin. When it get fullish, we place in the slide oven to melt the paraffin, when melted pour off, and reuse the cup... :) Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.cormier@crl.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, August 08, 2011 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Knife for trimming paraffin from blocks You Wrote: I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. ************************************************************************ * Dear Mala, In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer with a heated, slanted metal surface. This trimmer has made everyone happy by eliminating the potential for serious injury and does an even better job of getting rid of excess paraffin. There are two models sold, one from Thermo Scientific and the other one was recently spotted on a vendor website. (Sorry, I didn't jot the name down). The trimmers are worth the investment to keep you and your employees safe from nasty cuts, no matter what the knife/blade, etc could be used. The only drawback to the Para Trimmer is the messy paraffin drippings have to be collected in some separate container that the manufacturer doesn't supply. This is more than a bit annoying. It seems to me the manufacturer of these devices would design the trimmer with a paraffin catch tray. A small aluminum baking pan (from grocery store) could be used and disposed of (not a "Green" consideration). Be safe rather than sorry. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ArtimK <@t> slhn.org Mon Aug 8 10:07:22 2011 From: ArtimK <@t> slhn.org (Artim, Kimberly) Date: Mon Aug 8 10:07:27 2011 Subject: [Histonet] Department of Health Inspection Message-ID: <9E67FDD215B226448638018A82B952BD02563109BB9B@EXCHANGE.slhn.org> I was wondering if anyone could give me insight into the types of things a DOH inspector would be looking for during the inspection of a new lab located offsite? Any help would be much appreciated! --Kim Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From POWELL_SA <@t> mercer.edu Mon Aug 8 10:09:17 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Aug 8 10:09:25 2011 Subject: [Histonet] Re: Knife for trimming paraffin from blocks In-Reply-To: <895CFE180FF6704FBA7C0257D8DE344855D36D@ent-pr-xch-05.na01.crl.com> References: <000001cc55db$59f88600$0de99200$@bresnan.net> <895CFE180FF6704FBA7C0257D8DE344855D36D@ent-pr-xch-05.na01.crl.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DCB389E1@MERCERMAIL.MercerU.local> I too use the paratrimmer. It is great for avoiding repetitive injuries. For those of us who have carpal tunel syndrome and arthritis in the hands and cannot hold a small knife long enough to scrap multiple blocks it is great. Just part of my PPE. Worth every penny. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cormier, Kathleen Sent: Monday, August 08, 2011 11:04 AM To: gayle callis; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Knife for trimming paraffin from blocks We use the paratrimmer too. What we do for a paraffin catcher is to use a specimen cup (I call them urine cups, but whatever) to catch the paraffin. When it get fullish, we place in the slide oven to melt the paraffin, when melted pour off, and reuse the cup... :) Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.cormier@crl.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, August 08, 2011 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Knife for trimming paraffin from blocks You Wrote: I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. ************************************************************************ * Dear Mala, In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer with a heated, slanted metal surface. This trimmer has made everyone happy by eliminating the potential for serious injury and does an even better job of getting rid of excess paraffin. There are two models sold, one from Thermo Scientific and the other one was recently spotted on a vendor website. (Sorry, I didn't jot the name down). The trimmers are worth the investment to keep you and your employees safe from nasty cuts, no matter what the knife/blade, etc could be used. The only drawback to the Para Trimmer is the messy paraffin drippings have to be collected in some separate container that the manufacturer doesn't supply. This is more than a bit annoying. It seems to me the manufacturer of these devices would design the trimmer with a paraffin catch tray. A small aluminum baking pan (from grocery store) could be used and disposed of (not a "Green" consideration). Be safe rather than sorry. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Mon Aug 8 10:13:47 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Aug 8 10:13:57 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: References: Message-ID: Don't look in a catalog - go to Target or WalMart and look on the wall with kitchen gadgets. It will be much cheaper. On Aug 8, 2011, at 6:28 AM, wrote: > Hi All, > > I wonder if someone could help me with this! > > I am looking for a stainless steel knife that we use to scrape off the > paraffin from the embedded blocks. I could not find the 6 inches size > knife in any catalog. > > Thanks in advance > > Mala > > Nirmala Srishan > Histology Supervisor > Holy Name Medical Center. > > __________________ > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > Holy Name Medical Center is the recipient of: > > Magnet Recognition for Excellence in Patient Care, American Nurses > Credentialing Center > > 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern > Healthcare > > Best Places to Work in New Jersey, NJBIZ > > Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. > Power > > Distinguished Hospital Awards for Clinical Excellence, HealthGrades > > Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, > HealthGrades > > Best in Value Award, Data Advantage, LLC > > Chest Pain Center Accreditation, Society of Chest Pain Centers > > Primary Stroke Center Designation, The Joint Commission and NJ Department > of Health and Human Services > > > **** Warning: The information contained in this message is privileged and > CONFIDENTIAL and is intended only for the use of the addressee above. If > you are not the intended recipient, you are hereby notified that any > disclosure, copying, distribution, or taking of any action in reliance on > the content of this message is strictly prohibited. If you have received > this communication in error, please notify the sender by replying to this > message, and then delete it from your system. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From king.laurie <@t> marshfieldclinic.org Mon Aug 8 10:13:57 2011 From: king.laurie <@t> marshfieldclinic.org (King, Laurie) Date: Mon Aug 8 10:14:13 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks Message-ID: <201108081514.p78FE54J029990@mailhost2.mfldclin.edu> If you can't afford the melter, just go to Walmart or someplace similar, you can get inexpensive, small kitchen paring knives for less than $1 each. And, they are less likely to cause injury than a scalpel. Laurie ------Original Message------ From: "srishan@mail.holyname.org" Date: Mon Aug 08, 2011 -- 08:29:18 AM To: histonet-bounces@lists.utsouthwestern.edu,histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From jshelley <@t> sanfordburnham.org Mon Aug 8 10:14:54 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Mon Aug 8 10:15:01 2011 Subject: [Histonet] Re: Knife for trimming paraffin from blocks In-Reply-To: <895CFE180FF6704FBA7C0257D8DE344855D36D@ent-pr-xch-05.na01.crl.com> References: <000001cc55db$59f88600$0de99200$@bresnan.net> <895CFE180FF6704FBA7C0257D8DE344855D36D@ent-pr-xch-05.na01.crl.com> Message-ID: <5A605CE38EECB64B94485C02125A0C4405C73D72@LN-MAIL07.ln.burnham.org> Hi All, I likewise use the Para-trimmer and instead of a specimen cup I just use the top lid of a 1/2 gross microscope slide box. Once filled I just throw away in the trash. Kind Regards! ? John J Shelley Senior Research Associate, Histology Core -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cormier, Kathleen Sent: Monday, August 08, 2011 11:04 AM To: gayle callis; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Knife for trimming paraffin from blocks We use the paratrimmer too. What we do for a paraffin catcher is to use a specimen cup (I call them urine cups, but whatever) to catch the paraffin. When it get fullish, we place in the slide oven to melt the paraffin, when melted pour off, and reuse the cup... :) Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.cormier@crl.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, August 08, 2011 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Knife for trimming paraffin from blocks You Wrote: I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. ************************************************************************ * Dear Mala, In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer with a heated, slanted metal surface. This trimmer has made everyone happy by eliminating the potential for serious injury and does an even better job of getting rid of excess paraffin. There are two models sold, one from Thermo Scientific and the other one was recently spotted on a vendor website. (Sorry, I didn't jot the name down). The trimmers are worth the investment to keep you and your employees safe from nasty cuts, no matter what the knife/blade, etc could be used. The only drawback to the Para Trimmer is the messy paraffin drippings have to be collected in some separate container that the manufacturer doesn't supply. This is more than a bit annoying. It seems to me the manufacturer of these devices would design the trimmer with a paraffin catch tray. A small aluminum baking pan (from grocery store) could be used and disposed of (not a "Green" consideration). Be safe rather than sorry. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Mon Aug 8 10:15:04 2011 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon Aug 8 10:15:06 2011 Subject: [Histonet] Re: Knife for trimming paraffin from blocks In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238DCB389E1@MERCERMAIL.MercerU.local> Message-ID: <255504519.29090.1312816504178.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> If you are using the cassette writers and slide writers the knives can damage the surface of the barcode so I try to have everyone use the paratrimmers.? It is best not to have the repetitive injuries some of us developed over years of trimming blocks and many other things.? Pam Marcum UAMS ----- Original Message ----- From: "Shirley A. Powell" To: "Kathleen Cormier" , "gayle callis" , histonet@lists.utsouthwestern.edu Sent: Monday, August 8, 2011 10:09:17 AM Subject: RE: [Histonet] Re: ?Knife for trimming paraffin from blocks I too use the paratrimmer. ?It is great for avoiding repetitive injuries. ?For those of us who have carpal tunel syndrome and arthritis in the hands and cannot hold a small knife long enough to scrap multiple blocks it is great. ?Just part of my PPE. ?Worth every penny. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cormier, Kathleen Sent: Monday, August 08, 2011 11:04 AM To: gayle callis; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Knife for trimming paraffin from blocks We use the paratrimmer too. What we do for a paraffin catcher is to use a specimen cup (I call them urine cups, but whatever) to catch the paraffin. When it get fullish, we place in the slide oven to melt the paraffin, when melted pour off, and reuse the cup... :) Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.cormier@crl.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, August 08, 2011 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Knife for trimming paraffin from blocks You Wrote: ? I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. ?I could not find the 6 inches size knife in any catalog. ? Thanks in advance ? Mala ? Nirmala Srishan Histology Supervisor Holy Name Medical Center. ? ************************************************************************ * Dear Mala, ? In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer with a heated, slanted metal surface. ?This trimmer has made everyone happy by eliminating the potential for serious injury and does an even better job of getting rid of excess paraffin. ? ? ? There are two models sold, one from Thermo Scientific and the other one was recently spotted on a vendor website. (Sorry, I didn't jot the name down). The trimmers ?are worth the investment to keep you and your employees safe from nasty cuts, no matter what the knife/blade, etc could be used. ? ? The only drawback to the Para Trimmer is the messy paraffin drippings have to be collected in some separate container that the manufacturer doesn't supply. ?This is more than a bit annoying. ?It seems to me the manufacturer of these devices would design the trimmer with a paraffin catch tray. ?A small aluminum baking pan (from grocery store) could be used and disposed of (not a "Green" consideration). ? ? ? Be safe rather than sorry. ? ? Gayle M. Callis HTL/HT/MT(ASCP) ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Mon Aug 8 10:15:20 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Aug 8 10:15:22 2011 Subject: [Histonet] RE: knife used for cleaning paraffin off the blocks In-Reply-To: References: Message-ID: Mala, I use a dental knife used for trimming dentures. It is by Buffalo Dental. It has a wooden handle (similar to an old paring knife), but a small blade and fits great in my hands (which are kinda big). You can also try a company that sells dissecting instuments. Really what you are looking for is a scalpel or cartilage knife. I have recommended DR Instruments to my students. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. From algranth <@t> email.arizona.edu Mon Aug 8 10:26:58 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Aug 8 10:27:02 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: References: Message-ID: <3BAC8F82-A3FF-45CA-8DB1-594F1AF78FE7@email.arizona.edu> Actually we have a ParaTrimmer too - it was not that expensive and we love it. We paid @ $500 for ours and I see the they are a bit more expensive now - list price anyway. To solve the drip problem I put one of the tops from the boxes of pipette tips under the ledge and it catches all the drips. When the paraffin hardens you can just pop it out and when the "drip catcher" gets nasty we just throw it away and get another one. On Aug 8, 2011, at 8:13 AM, Grantham, Andrea L - (algranth) wrote: > Don't look in a catalog - go to Target or WalMart and look on the wall with kitchen gadgets. It will be much cheaper. > > > > On Aug 8, 2011, at 6:28 AM, wrote: > >> Hi All, >> >> I wonder if someone could help me with this! >> >> I am looking for a stainless steel knife that we use to scrape off the >> paraffin from the embedded blocks. I could not find the 6 inches size >> knife in any catalog. >> >> Thanks in advance >> >> Mala >> >> Nirmala Srishan >> Histology Supervisor >> Holy Name Medical Center. >> >> __________________ >> >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >> Holy Name Medical Center is the recipient of: >> >> Magnet Recognition for Excellence in Patient Care, American Nurses >> Credentialing Center >> >> 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern >> Healthcare >> >> Best Places to Work in New Jersey, NJBIZ >> >> Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. >> Power >> >> Distinguished Hospital Awards for Clinical Excellence, HealthGrades >> >> Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, >> HealthGrades >> >> Best in Value Award, Data Advantage, LLC >> >> Chest Pain Center Accreditation, Society of Chest Pain Centers >> >> Primary Stroke Center Designation, The Joint Commission and NJ Department >> of Health and Human Services >> >> >> **** Warning: The information contained in this message is privileged and >> CONFIDENTIAL and is intended only for the use of the addressee above. If >> you are not the intended recipient, you are hereby notified that any >> disclosure, copying, distribution, or taking of any action in reliance on >> the content of this message is strictly prohibited. If you have received >> this communication in error, please notify the sender by replying to this >> message, and then delete it from your system. >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From JMacDonald <@t> mtsac.edu Mon Aug 8 10:30:19 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Aug 8 10:30:25 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: <3BAC8F82-A3FF-45CA-8DB1-594F1AF78FE7@email.arizona.edu> Message-ID: If you put a piece of paper towel in the bottom of the "drip catcher" you just change that and it makes it much easier. "Grantham, Andrea L - (algranth)" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/08/2011 08:28 AM To cc HISTONET Subject Re: [Histonet] knife used for cleaning paraffin off the blocks Actually we have a ParaTrimmer too - it was not that expensive and we love it. We paid @ $500 for ours and I see the they are a bit more expensive now - list price anyway. To solve the drip problem I put one of the tops from the boxes of pipette tips under the ledge and it catches all the drips. When the paraffin hardens you can just pop it out and when the "drip catcher" gets nasty we just throw it away and get another one. On Aug 8, 2011, at 8:13 AM, Grantham, Andrea L - (algranth) wrote: > Don't look in a catalog - go to Target or WalMart and look on the wall with kitchen gadgets. It will be much cheaper. > > > > On Aug 8, 2011, at 6:28 AM, wrote: > >> Hi All, >> >> I wonder if someone could help me with this! >> >> I am looking for a stainless steel knife that we use to scrape off the >> paraffin from the embedded blocks. I could not find the 6 inches size >> knife in any catalog. >> >> Thanks in advance >> >> Mala >> >> Nirmala Srishan >> Histology Supervisor >> Holy Name Medical Center. >> >> __________________ >> >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >> Holy Name Medical Center is the recipient of: >> >> Magnet Recognition for Excellence in Patient Care, American Nurses >> Credentialing Center >> >> 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern >> Healthcare >> >> Best Places to Work in New Jersey, NJBIZ >> >> Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. >> Power >> >> Distinguished Hospital Awards for Clinical Excellence, HealthGrades >> >> Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, >> HealthGrades >> >> Best in Value Award, Data Advantage, LLC >> >> Chest Pain Center Accreditation, Society of Chest Pain Centers >> >> Primary Stroke Center Designation, The Joint Commission and NJ Department >> of Health and Human Services >> >> >> **** Warning: The information contained in this message is privileged and >> CONFIDENTIAL and is intended only for the use of the addressee above. If >> you are not the intended recipient, you are hereby notified that any >> disclosure, copying, distribution, or taking of any action in reliance on >> the content of this message is strictly prohibited. If you have received >> this communication in error, please notify the sender by replying to this >> message, and then delete it from your system. >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srishan <@t> mail.holyname.org Mon Aug 8 10:32:54 2011 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Mon Aug 8 10:33:17 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks Message-ID: Thank you to all who have responded to my question. I do greatly appreciate it ! Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From POWELL_SA <@t> mercer.edu Mon Aug 8 10:33:47 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Aug 8 10:33:52 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks In-Reply-To: References: <3BAC8F82-A3FF-45CA-8DB1-594F1AF78FE7@email.arizona.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DCB38A41@MERCERMAIL.MercerU.local> I use a glass staining dish to catch the drippings. When full I place it in the freezer to harden and it comes right out and I toss it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, August 08, 2011 11:30 AM To: Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] knife used for cleaning paraffin off the blocks If you put a piece of paper towel in the bottom of the "drip catcher" you just change that and it makes it much easier. "Grantham, Andrea L - (algranth)" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/08/2011 08:28 AM To cc HISTONET Subject Re: [Histonet] knife used for cleaning paraffin off the blocks Actually we have a ParaTrimmer too - it was not that expensive and we love it. We paid @ $500 for ours and I see the they are a bit more expensive now - list price anyway. To solve the drip problem I put one of the tops from the boxes of pipette tips under the ledge and it catches all the drips. When the paraffin hardens you can just pop it out and when the "drip catcher" gets nasty we just throw it away and get another one. On Aug 8, 2011, at 8:13 AM, Grantham, Andrea L - (algranth) wrote: > Don't look in a catalog - go to Target or WalMart and look on the wall with kitchen gadgets. It will be much cheaper. > > > > On Aug 8, 2011, at 6:28 AM, wrote: > >> Hi All, >> >> I wonder if someone could help me with this! >> >> I am looking for a stainless steel knife that we use to scrape off the >> paraffin from the embedded blocks. I could not find the 6 inches size >> knife in any catalog. >> >> Thanks in advance >> >> Mala >> >> Nirmala Srishan >> Histology Supervisor >> Holy Name Medical Center. >> >> __________________ >> >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >> Holy Name Medical Center is the recipient of: >> >> Magnet Recognition for Excellence in Patient Care, American Nurses >> Credentialing Center >> >> 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern >> Healthcare >> >> Best Places to Work in New Jersey, NJBIZ >> >> Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. >> Power >> >> Distinguished Hospital Awards for Clinical Excellence, HealthGrades >> >> Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, >> HealthGrades >> >> Best in Value Award, Data Advantage, LLC >> >> Chest Pain Center Accreditation, Society of Chest Pain Centers >> >> Primary Stroke Center Designation, The Joint Commission and NJ Department >> of Health and Human Services >> >> >> **** Warning: The information contained in this message is privileged and >> CONFIDENTIAL and is intended only for the use of the addressee above. If >> you are not the intended recipient, you are hereby notified that any >> disclosure, copying, distribution, or taking of any action in reliance on >> the content of this message is strictly prohibited. If you have received >> this communication in error, please notify the sender by replying to this >> message, and then delete it from your system. >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From technicalsupport <@t> newcomersupply.com Mon Aug 8 10:35:29 2011 From: technicalsupport <@t> newcomersupply.com (Technical Support) Date: Mon Aug 8 10:35:44 2011 Subject: [Histonet] RE: Histonet Digest, Vol 93, Issue 9 In-Reply-To: <20110808145924.522F220D458@barracuda.5ninesdata.com> References: <20110808145924.522F220D458@barracuda.5ninesdata.com> Message-ID: Hi Mala, Newcomer Supply has a Paraffin Wax Trimmer with a container to catch the paraffin. JoAnn M Szczepaniuk, HT (ASCP) Technical Support Newcomer Supply 2505 Parview Road Middleton, Wi. 53562 800-383-7799 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, August 08, 2011 9:59 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 93, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. 20 slide holder for Microprobe system (Rob Geske) 2. problems posting (Heckford, Karen - SMMC-SF) 3. Per diem Position in the San Francisco Bay Area (Heckford, Karen - SMMC-SF) 4. RE: histology equipment service (Carol Fields) 5. knife used for cleaning paraffin off the blocks (srishan@mail.holyname.org) 6. RE: knife used for cleaning paraffin off the blocks (Breeden, Sara) 7. Re: knife used for cleaning paraffin off the blocks (Rene J Buesa) 8. RE: knife used for cleaning paraffin off the blocks (Horn, Hazel V) 9. re-cycled xylene in tissue processor (Gaiser, Marcia) 10. Re: re-cycled xylene in tissue processor (Rene J Buesa) 11. knife used for cleaning paraffin off the blocks (Paula Pierce) 12. RE: knife used for cleaning paraffin off the blocks (Rathborne, Toni) 13. RE: knife used for cleaning paraffin off the blocks (Sheila Fonner) 14. RE: knife used for cleaning paraffin off the blocks (Bartlett, Jeanine (CDC/OID/NCEZID)) 15. RE: re-cycled xylene in tissue processor (Laurie Colbert) 16. RE: knife used for cleaning paraffin off the blocks (Jeanne Estabel) 17. Re: Knife for trimming paraffin from blocks (gayle callis) ---------------------------------------------------------------------- Message: 1 Date: Sun, 7 Aug 2011 10:38:58 -0700 (PDT) From: Rob Geske Subject: [Histonet] 20 slide holder for Microprobe system To: "histonet@lists.utsouthwestern.edu" Message-ID: <1312738738.68756.YahooMailNeo@web39410.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 All, ? i'm looking for a supplier of the 20 slide holder/handle for the microprobe staining system previously sold by fisher.?fisher has discontinue this as too has cole palmer.? the entire system is available on e-bay and also through some used equipment re-sellers, but i just?need the handle.? any help would be appreciated. ? thanks in advance, rob ------------------------------ Message: 2 Date: Mon, 8 Aug 2011 05:10:57 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] problems posting To: Message-ID: <9ECF174E7DA83046BE6EBFDE009E28A388C413@CHW-MSG-301.chw.edu> Content-Type: text/plain; charset="us-ascii" I am having problems posting to Histonet. Not sure what is going on. Hopefully this goes through if not please add me to your list. I have been on the the Histonet for 7 years now. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 616 7Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 3 Date: Mon, 8 Aug 2011 05:19:24 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Per diem Position in the San Francisco Bay Area To: Message-ID: <9ECF174E7DA83046BE6EBFDE009E28A388C414@CHW-MSG-301.chw.edu> Content-Type: text/plain; charset="us-ascii" We are looking for a Certified Histology Technician for per diem work. You will also do some Pathology Assisting (nothing to extensive on the PA). Need someone that can cover vacation, sick days, etc. Perfect for someone that is retired and is looking for some extra money. Need to be able to work on your own independently and know how to troubleshoot both Histology and IHC's. You will need to be able to be available to work up to 1-2 weeks at a time or get called in at the last minute in case someone is sick. So a flexible schedule is ideal. If interested please call and leave me a message. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 616 7Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 4 Date: Mon, 8 Aug 2011 08:48:20 -0400 From: "Carol Fields" Subject: RE: [Histonet] histology equipment service To: "Shirley A. Powell" Cc: histonet@lists.utsouthwestern.edu Message-ID: <731941C266951A47BEF11E5EFAAED9C90B666B7C@nsmvexch01.northside.local> Content-Type: text/plain;charset="us-ascii" Southeast Pathology Services, Inc. we have used for years and do an excellent job. They also have refurbed equipment. Call Michael Dietrich at 843-588-2559 Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, August 04, 2011 5:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histology equipment service For the person/persons looking for someone to do equipment maintenance/service I can highly recommend this company, Pathology Service, Inc. Sean Draime and Joel DeVisser are very knowledgeable on all types of histology equipment and their charges are reasonable. They are in Georgia, of course, GO DAWGS, but travel all over the country doing repair, PM, and also have used equipment now. Their number is 1-866-398-9478, or contact them by going to www.pathologyserv.com. Also see email below for more information on equipment purchases. To: Shirley A. Powell Subject: Joel from PSI Hi, this is my email here for any info you come across reference people who may need to sell any lab equipment or labs. We also do offer very competitive service and preventative maintenance packages and cover most of the USA. Sean has 3 different plans that can be customized to fit any clients needs or budgets. We also sell used, refurbished equipment and our pieces go through frame off restorations and come out working and looking like new again. We offer 90 day warranties on everything we sell and 1 year extensions all through PSI. Thank you so much for the information on the news letter and for taking the time to speak with me earlier on the phone. Joel DeVisser 678-887-6068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ Message: 5 Date: Mon, 8 Aug 2011 06:28:33 -0700 From: srishan@mail.holyname.org Subject: [Histonet] knife used for cleaning paraffin off the blocks To: histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ------------------------------ Message: 6 Date: Mon, 8 Aug 2011 07:46:50 -0600 From: "Breeden, Sara" Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks To: , Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF967@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" AHA! I have an Answer! EMS (Electron Microscopy Sciences) sells a "One-Piece Scalpel Blade with Handle" Cat. #72048-01 that is perfect for cleaning blocks of excess paraffin! I've had one for over 30 years and it will retire with me! It is initially sharp (but not as sharp as a normal blade would be) but if you cut open a few cardboard boxes with it (which it also does beautifully), it "dulls down" to the perfect edge for cleaning blocks. It fits the hand well and doesn't shave the plastic off the cassette. Someone left an old paring knife here but it's too big and cumbersome. ------------------------------ Message: 7 Date: Mon, 8 Aug 2011 06:58:47 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] knife used for cleaning paraffin off the blocks To: histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, srishan@mail.holyname.org Message-ID: <1312811927.72000.YahooMailClassic@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I always used my Wenger Swiss Army pocket knife. Ren? J. --- On Mon, 8/8/11, srishan@mail.holyname.org wrote: From: srishan@mail.holyname.org Subject: [Histonet] knife used for cleaning paraffin off the blocks To: histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu Date: Monday, August 8, 2011, 9:28 AM Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks.? I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 8 Aug 2011 09:32:08 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks To: "'srishan@mail.holyname.org'" , "histonet-bounces@lists.utsouthwestern.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB7198B85AD60@EVS1.archildrens.org> Content-Type: text/plain; charset="us-ascii" We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 9 Date: Mon, 8 Aug 2011 09:33:43 -0500 From: "Gaiser, Marcia" Subject: [Histonet] re-cycled xylene in tissue processor To: "histonet@lists.utsouthwestern.edu" Message-ID: <728F817C02110E498D803A7C3B0C6248068D43B5E0@S009-APEXM06.ds.ad.ssmhc.com> Content-Type: text/plain; charset="iso-8859-1" Hi, Has anyone had experience using re-cycled xylene in the Tissue-Tek VIP5 tissue processor for the clean cycle? Will use of re-cycled xylene, over time, damage the processor? Thank you, Marcia Gaiser Pathology Supervisor Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ------------------------------ Message: 10 Date: Mon, 8 Aug 2011 07:45:08 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] re-cycled xylene in tissue processor To: "histonet@lists.utsouthwestern.edu" , MarciaGaiser Message-ID: <1312814708.22223.YahooMailClassic@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If you xylene recycler works according to standard and maintained properly, the recycled xylene you obtain is of equal quality to the xylene you buy from your usual supplier. It can be used in any and all steps of your processing and staining protocols, and there is no way it could "damage" your or any other tissue processing using xylene. All the "difficulties" with recycled xylene are caused by improper recycling. Ren? J. --- On Mon, 8/8/11, Gaiser, Marcia wrote: From: Gaiser, Marcia Subject: [Histonet] re-cycled xylene in tissue processor To: "histonet@lists.utsouthwestern.edu" Date: Monday, August 8, 2011, 10:33 AM Hi, Has anyone had experience using re-cycled xylene in the Tissue-Tek VIP5 tissue processor for the clean cycle? Will use of re-cycled xylene, over time, damage the processor? Thank you, Marcia Gaiser Pathology Supervisor Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 8 Aug 2011 07:46:31 -0700 (PDT) From: Paula Pierce Subject: [Histonet] knife used for cleaning paraffin off the blocks To: Histonet Message-ID: <1312814791.72524.YahooMailRC@web1113.biz.mail.sk1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I just use the handle end of my forceps. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Horn, Hazel V" To: "srishan@mail.holyname.org" ; "histonet-bounces@lists.utsouthwestern.edu" ; "histonet@lists.utsouthwestern.edu" Sent: Mon, August 8, 2011 9:32:08 AM Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way? ? Slot 820 Little Rock, AR? 72202 phone? 501.364.4240 fax? ? ? ? 501.364.3155 visit us on the web at:? ? www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks.? I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 8 Aug 2011 14:50:25 +0000 From: "Rathborne, Toni" Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks To: "'Paula Pierce'" , Histonet Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F509F0@SMCMAIL01.somerset-healthcare.com> Content-Type: text/plain; charset="utf-8" I usually use one of the embedding molds. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 08, 2011 10:47 AM To: Histonet Subject: [Histonet] knife used for cleaning paraffin off the blocks I just use the handle end of my forceps. ?? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Horn, Hazel V" To: "srishan@mail.holyname.org" ; "histonet-bounces@lists.utsouthwestern.edu" ; "histonet@lists.utsouthwestern.edu" Sent: Mon, August 8, 2011 9:32:08 AM Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way?? ?? Slot 820 Little Rock, AR?? 72202 phone?? 501.364.4240 fax?? ?? ?? ?? 501.364.3155 visit us on the web at:?? ?? www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks.?? I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 13 Date: Mon, 8 Aug 2011 10:47:54 -0400 From: "Sheila Fonner" Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks To: Message-ID: <003f01cc55da$27917f60$76b47e20$@com> Content-Type: text/plain; charset="us-ascii" Since I haven't seen anyone talk about this yet, I feel obligated to tell you all that there is a handy dandy little "melter" out there for getting the excess paraffin off the blocks. You just run the edges of the block over it and voila...trimmed. When you have up to 600 blocks a day, this thing is wonderful. And it keeps your hands from cramping up. Oh the wonders!! Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, August 08, 2011 10:32 AM To: 'srishan@mail.holyname.org'; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 8 Aug 2011 14:52:21 +0000 From: "Bartlett, Jeanine (CDC/OID/NCEZID)" Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks To: "Rathborne, Toni" , 'Paula Pierce' , Histonet Message-ID: Content-Type: text/plain; charset="utf-8" I don't have much paraffin on the edges of the block but if I do I pop it off with the edge of my finger nail...... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Monday, August 08, 2011 10:50 AM To: 'Paula Pierce'; Histonet Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks I usually use one of the embedding molds. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 08, 2011 10:47 AM To: Histonet Subject: [Histonet] knife used for cleaning paraffin off the blocks I just use the handle end of my forceps. ?? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Horn, Hazel V" To: "srishan@mail.holyname.org" ; "histonet-bounces@lists.utsouthwestern.edu" ; "histonet@lists.utsouthwestern.edu" Sent: Mon, August 8, 2011 9:32:08 AM Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way?? ?? Slot 820 Little Rock, AR?? 72202 phone?? 501.364.4240 fax?? ?? ?? ?? 501.364.3155 visit us on the web at:?? ?? www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks.?? I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 15 Date: Mon, 8 Aug 2011 07:53:42 -0700 From: "Laurie Colbert" Subject: RE: [Histonet] re-cycled xylene in tissue processor To: "Gaiser, Marcia" , Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AF7D@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" We use recycled xylene for the cleaning cycle on our VIP 5's and have had no problems. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gaiser, Marcia Sent: Monday, August 08, 2011 7:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re-cycled xylene in tissue processor Hi, Has anyone had experience using re-cycled xylene in the Tissue-Tek VIP5 tissue processor for the clean cycle? Will use of re-cycled xylene, over time, damage the processor? Thank you, Marcia Gaiser Pathology Supervisor Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 8 Aug 2011 15:55:00 +0100 From: "Jeanne Estabel" Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks To: Message-ID: <34627BB49F43DC43A4A7D90B1B6F739D0115E1AB@exchsrv4.internal.sanger.ac.uk> Content-Type: text/plain; charset="us-ascii" Hi, Here we are using the "melter" or the knife depends on the technician and the number of blocks. Regards Jeanne Jeanne Estabel, PhD Scientific Manager Histology Operations Manager Mouse Genetics Project Wellcome Trust Sanger Institute Cambridge, UK Tel:+44 (0)1223 834244 ext 8306 Find Sanger Mouse Genetics Project phenotyping data on http://www.sanger.ac.uk/mouseportal/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: 08 August 2011 15:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks Since I haven't seen anyone talk about this yet, I feel obligated to tell you all that there is a handy dandy little "melter" out there for getting the excess paraffin off the blocks. You just run the edges of the block over it and voila...trimmed. When you have up to 600 blocks a day, this thing is wonderful. And it keeps your hands from cramping up. Oh the wonders!! Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, August 08, 2011 10:32 AM To: 'srishan@mail.holyname.org'; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] knife used for cleaning paraffin off the blocks We use a couple of butter knives we brought from home. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Monday, August 08, 2011 8:29 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] knife used for cleaning paraffin off the blocks Hi All, I wonder if someone could help me with this! I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ************************************************************************ **** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE. ------------------------------ Message: 17 Date: Mon, 8 Aug 2011 08:56:25 -0600 From: "gayle callis" Subject: [Histonet] Re: Knife for trimming paraffin from blocks To: Message-ID: <000001cc55db$59f88600$0de99200$@bresnan.net> Content-Type: text/plain; charset="us-ascii" You Wrote: I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. ************************************************************************* Dear Mala, In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer with a heated, slanted metal surface. This trimmer has made everyone happy by eliminating the potential for serious injury and does an even better job of getting rid of excess paraffin. There are two models sold, one from Thermo Scientific and the other one was recently spotted on a vendor website. (Sorry, I didn't jot the name down). The trimmers are worth the investment to keep you and your employees safe from nasty cuts, no matter what the knife/blade, etc could be used. The only drawback to the Para Trimmer is the messy paraffin drippings have to be collected in some separate container that the manufacturer doesn't supply. This is more than a bit annoying. It seems to me the manufacturer of these devices would design the trimmer with a paraffin catch tray. A small aluminum baking pan (from grocery store) could be used and disposed of (not a "Green" consideration). Be safe rather than sorry. Gayle M. Callis HTL/HT/MT(ASCP) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 93, Issue 9 *************************************** From histotech <@t> imagesbyhopper.com Mon Aug 8 10:46:55 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Aug 8 10:48:47 2011 Subject: [Histonet] Re: Knife for trimming paraffin from blocks In-Reply-To: <5A605CE38EECB64B94485C02125A0C4405C73D72@LN-MAIL07.ln.burnham.org> References: <000001cc55db$59f88600$0de99200$@bresnan.net> <895CFE180FF6704FBA7C0257D8DE344855D36D@ent-pr-xch-05.na01.crl.com> <5A605CE38EECB64B94485C02125A0C4405C73D72@LN-MAIL07.ln.burnham.org> Message-ID: <7AC5A3C6-0AAD-434A-962D-5F44B523EBE9@imagesbyhopper.com> I agree, the Para-Trimmer is the next best thing since sliced bread! I'll never go back to the knife again. We use lid from one of the Ventana special stains boxes to catch the wax. I have also fashioned a drip tray out of the cardboard boxes that the coverslips come in. We're all McGuyver's at heart, aren't we? :o) Sent from my iPhone On Aug 8, 2011, at 11:14 AM, John Shelley wrote: > Hi All, > > I likewise use the Para-trimmer and instead of a specimen cup I just use the top lid of a 1/2 gross microscope slide box. Once filled I just throw away in the trash. > > Kind Regards! > > John J Shelley > Senior Research Associate, Histology Core > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cormier, Kathleen > Sent: Monday, August 08, 2011 11:04 AM > To: gayle callis; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Knife for trimming paraffin from blocks > > We use the paratrimmer too. What we do for a paraffin catcher is to use > a specimen cup (I call them urine cups, but whatever) to catch the > paraffin. When it get fullish, we place in the slide oven to melt the > paraffin, when melted pour off, and reuse the cup... :) > > Kathy Cormier > Histology Manager > Charles River Laboratories > 251 Ballardvale Street > Wilmington, MA 01887 > Ph: 781-222-6803 > Fax: 978-988-8793 > kathleen.cormier@crl.com > Accelerating Drug Development. Exactly. > Notice - This email and any files transmitted with it are confidential > and may contain privileged and/or proprietary information. You must not > disclose this message to another party without Charles River's express > written consent. If you are not the intended recipient you must not > copy, distribute or use this email or the information contained in it > for any purpose other than to notify us. If you have received this > message in error, please notify Charles River immediately, and delete it > from your system. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle > callis > Sent: Monday, August 08, 2011 10:56 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Knife for trimming paraffin from blocks > > You Wrote: > > > > I am looking for a stainless steel knife that we use to scrape off the > > paraffin from the embedded blocks. I could not find the 6 inches size > > knife in any catalog. > > > > Thanks in advance > > > > Mala > > > > Nirmala Srishan > > Histology Supervisor > > Holy Name Medical Center. > > > > ************************************************************************ > * > > Dear Mala, > > > > In the past, we used an old style permanent edge scalpel blade but after > a > disaster with one person seirously cutting himself while trimming > paraffin > from a block, we purchased a Paratrimmer with a heated, slanted metal > surface. This trimmer has made everyone happy by eliminating the > potential > for serious injury and does an even better job of getting rid of excess > paraffin. > > > > There are two models sold, one from Thermo Scientific and the other one > was > recently spotted on a vendor website. (Sorry, I didn't jot the name > down). > The trimmers are worth the investment to keep you and your employees > safe > from nasty cuts, no matter what the knife/blade, etc could be used. > > > > The only drawback to the Para Trimmer is the messy paraffin drippings > have > to be collected in some separate container that the manufacturer doesn't > supply. This is more than a bit annoying. It seems to me the > manufacturer > of these devices would design the trimmer with a paraffin catch tray. A > small aluminum baking pan (from grocery store) could be used and > disposed of > (not a "Green" consideration). > > > > Be safe rather than sorry. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jclark <@t> pcnm.com Mon Aug 8 11:01:19 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon Aug 8 11:01:23 2011 Subject: [Histonet] RE: Histonet Digest, Vol 93, Issue 9 In-Reply-To: <20110808150046.B45D2A7D495@mx10.myoutlookonline.com> References: <20110808150046.B45D2A7D495@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E3786EA635@S10MAILD001N1.SH10.lan> We have been using recycled xylene on our VIP5 for the past 5 years and haven't had any problems. We use it for both the cleaning cycles and the xylene stations. Joanne Clark, HT Histology Supervisor PCNM ------------------------------ Message: 9 Date: Mon, 8 Aug 2011 09:33:43 -0500 From: "Gaiser, Marcia" Subject: [Histonet] re-cycled xylene in tissue processor To: "histonet@lists.utsouthwestern.edu" Message-ID: <728F817C02110E498D803A7C3B0C6248068D43B5E0@S009-APEXM06.ds.ad.ssmhc.com> Content-Type: text/plain; charset="iso-8859-1" Hi, Has anyone had experience using re-cycled xylene in the Tissue-Tek VIP5 tissue processor for the clean cycle? Will use of re-cycled xylene, over time, damage the processor? Thank you, Marcia Gaiser Pathology Supervisor Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ------------------------------ From Lisa.White3 <@t> va.gov Mon Aug 8 11:10:34 2011 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Mon Aug 8 11:10:39 2011 Subject: : [Histonet] re-cycled xylene in tissue processor Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760874200A@VHAV09MSGA3.v09.med.va.gov> We have recycled xylene in the past and now Formula 83 and have a VIP 5 and VIP 6. Sakura told us when we purchased each processor NOT to use recycled solvent in Sakura processors for the cleaning solvent station. If you want to give it a try would suggest waiting until the warranty has expired on the off chance that something would go wrong. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From ctorrence <@t> kmcpa.com Mon Aug 8 12:32:14 2011 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Mon Aug 8 12:32:42 2011 Subject: [Histonet] knife used for cleaning paraffin off the blocks Message-ID: <001201cc55f1$1cbf4c40$563de4c0$@com> For years I have used a palette knife used for mixing and painting with any artist media. You will find it in the art section of any art/craft store. They have a handle and a flexible blade that does not have a cutting edge on it. I have had the same one for years. If you have a heavy workload, just wrap masking tape around the area that your index finger comes in contact with the blade. Carol M. Torrence, HT(ASCP) KMC Dermatology - Laboratory 2921 SW Wanamaker Dr. Topeka, Kansas 66614-5334 785-273-2788 ext 328 fax 785-272-6185 ctorrence@kmcpa.com Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you From JWeems <@t> sjha.org Mon Aug 8 14:52:06 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Aug 8 14:52:12 2011 Subject: [Histonet] Re: Knife for trimming paraffin from blocks In-Reply-To: <000001cc55db$59f88600$0de99200$@bresnan.net> References: <000001cc55db$59f88600$0de99200$@bresnan.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408277BD4ED@CHEXCMS10.one.ads.che.org> Hey ya'll, You can also melt the paraffin on the embedding center... No extra cost and it comes with its own drip tray!! :>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, August 08, 2011 10:56 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Knife for trimming paraffin from blocks You Wrote: I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. ************************************************************************* Dear Mala, In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer with a heated, slanted metal surface. This trimmer has made everyone happy by eliminating the potential for serious injury and does an even better job of getting rid of excess paraffin. There are two models sold, one from Thermo Scientific and the other one was recently spotted on a vendor website. (Sorry, I didn't jot the name down). The trimmers are worth the investment to keep you and your employees safe from nasty cuts, no matter what the knife/blade, etc could be used. The only drawback to the Para Trimmer is the messy paraffin drippings have to be collected in some separate container that the manufacturer doesn't supply. This is more than a bit annoying. It seems to me the manufacturer of these devices would design the trimmer with a paraffin catch tray. A small aluminum baking pan (from grocery store) could be used and disposed of (not a "Green" consideration). Be safe rather than sorry. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From wgray19 <@t> sc.rr.com Mon Aug 8 18:31:03 2011 From: wgray19 <@t> sc.rr.com (Jeff and Wanda Gray) Date: Mon Aug 8 18:32:17 2011 Subject: [Histonet] Re:Microtome Message-ID: <000001cc5623$3d7825b0$b8687110$@rr.com> Amita and all, Love my Microm 355S, and automated beats manual, hands down. (Pardon the pun) Automated has allowed me many years of freedom from repetitive motion injuries. Been in the field, let's just say"amny" years, and am only just now developing problems, while older colleagues who didn't have the benefit of automation suffer and have surgery. I know my day will come with computer use and duties other than cutting, but automation is awesome! Wanda Shotsberger Gray HT/HTL (ASCP), QIHC Hello friends, I am planning to buy a microtome for my lab ....i already have Leica manual one which is working perfectly. Now I wish to introduce a fully motorized one. Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME. Let me know your frank review on 1. manual versus automated option 2. Above all three items.in terms of their performance, maintenance and breakdown etc. or due you suggest any more better option Thanks in advance for your feed back. In past also histonetters helped me to solve many problems, looking forward this time also. Amita From nelsonrnch <@t> verizon.net Mon Aug 8 20:19:22 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Mon Aug 8 20:19:25 2011 Subject: [Histonet] Medite Equipment Message-ID: <1312852762.95631.YahooMailRC@web84306.mail.re1.yahoo.com> Does anyone?have an opinion good or bad on?Medite Equipment??Specifically a TPC 15 Tissue Processor or Histo Pro 3030 Robotic Slide Stainer. And if?so, who does the servicing on the equipment. ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net From mousecatchr <@t> aol.com Mon Aug 8 20:51:25 2011 From: mousecatchr <@t> aol.com (Mousecatcher) Date: Mon Aug 8 20:51:29 2011 Subject: [Histonet] Microm DS-50 slide stainer Message-ID: <8CE244DFB32484C-15D0-271C3@webmail-m007.sysops.aol.com> I bought a new Microm DS-50 slide stainer but never set it up. Is there a lab in need of this stainer? Contact off list if interested. From Vickroy.Jim <@t> mhsil.com Tue Aug 9 08:10:45 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Aug 9 08:11:07 2011 Subject: [Histonet] picture of gross lab senior Message-ID: <24A4826E8EF0964D86BC5317306F58A55DF753DD04@mmc-mail.ad.mhsil.com> No instructions sent to install a thin monitor/keyboard bracket on a gross lab senior. Can anyone send me a photo so we can match it up. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From talulahgosh <@t> gmail.com Tue Aug 9 08:54:27 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Aug 9 08:54:30 2011 Subject: [Histonet] picture of gross lab senior In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55DF753DD04@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A55DF753DD04@mmc-mail.ad.mhsil.com> Message-ID: gross lab senior = obese elderly technician? i don't think s/he would appreciate you installing a keyboard on said person. emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Aug 9, 2011 at 9:10 AM, Vickroy, Jim wrote: > No instructions sent to install a thin monitor/keyboard bracket on a gross > lab senior. Can anyone send me a photo so we can match it up. > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brian <@t> prometheushealthcare.com Tue Aug 9 09:14:09 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Tue Aug 9 09:14:23 2011 Subject: [Histonet] Grossing tech openings in Westchester Cty NY Message-ID: <008c01cc569e$9ea31090$dbe931b0$@com> Please contact me today for immediate consideration. Both day and night shift available. If you might know anyone who would be interested, that would be great as well! Thanks! POSITION SUMMARY Perform duties that relate to the histology specimen grossing of surgically removed tissue under the supervision of the Pathology Assistant / Pathologist and/or laboratory supervisor. ESSENTIAL FUNCTIONS * Ensure specimen integrity upon arrival and through out the entire process. * Dictate a gross description for each biopsy specimen, dissect and prepare tissue for cassettes. * Gross core needle biopsy and all anatomical pathology specimens (under the supervision of a Pathologist Assistant and /or pathologist). * Prepare dissected tissue specimens and place into cassettes. * Load prepared cassettes in Tissue Processors. * Operation of all tissue processors. * Familiar with safety procedures and standard operating procedures. * Compliance with all Federal and State regulations pertaining to Grossing: * Adhere to workload requirements. * Maintenance of accurate work records (i.e., Maintenance Logs, Filter Logs, Bench Excerpts etc.). * Able to evaluate new laboratory equipment and procedures. * Able to perform Quality Control and Quality Assurance Procedures. * Working knowledge and understanding of TPS / Lab Manager IS System. * Assist in the professional development of laboratory aides. * Daily housekeeping. POSITION REQUIREMENTS . Associated Degree in Biology and Chemistry; or successful completion of a NAACLS Accredited Histotechnology Program; or Associate Degree with at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university with a combination of 12 semester hours (18 quarter hours) of biology and chemistry. Must be NY State License for CLT or HT. . 3-4 Years as a grossing technician * Must be able to work with hazardous chemicals. DESIRED * Knowledgeable in the handling of specimens from accessioning to final specimen storage. * Working knowledge of laboratory organization, operational procedures, Quality Control, Quality Assurance, and laboratory safety. * Working knowledge and understanding of computers. * Cooperative working relationships with peers and management. * Dependable, flexible and adaptable in all aspects of work. * * * * * * * * * * Brian Feldman * Principal * Prometheus Healthcare * Office 301-693-9057 * Fax 301-368-2478 * brian@prometheushealthcare.com * www.prometheushealthcare.com * *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** * http://twitter.com/PrometheusBlog From Kathleen.Cormier <@t> crl.com Tue Aug 9 09:30:03 2011 From: Kathleen.Cormier <@t> crl.com (Cormier, Kathleen) Date: Tue Aug 9 09:30:13 2011 Subject: [Histonet] Job available in Massachusetts Message-ID: <895CFE180FF6704FBA7C0257D8DE344855D493@ent-pr-xch-05.na01.crl.com> Charles River Laboratories has an immediate opening in Wilmington Massachusetts: Please apply directly on the website, if interested in this job. Job Description Job Title: Technologist, Histology Job ID: 110681 Location: USA - MA - Wilmington Full/Part Time: Full-Time Regular/Temporary: Regular ________________________________ ________________________________ Responsibilities: BASIC SUMMARY: This position is responsible for performing basic histology procedures in the laboratory. ESSENTIAL DUTIES AND RESPONSIBILITIES: * Adhere to Good Laboratory Practice documentation policies while performing all histology lab procedures. * Follow all established departmental Standard Operating Procedures (SOPs) and laboratory safety policies. * Coordinate and file all procedural paperwork. * Prepare basic reagents and stains used in the laboratory. * Identify, locate and trim normal and abnormal formalin fixed tissue. * Trim and decalcify small animal bone specimens. * Embed single and multiple tissues. * Microtome section single and multiple embedded tissue paraffin blocks. * Stain slides for hematoxylin and eosin both manually and by using automated staining equipment. * Manually perform special histochemical stains (e.g. Gram, Warthin Starry, GMS). * Coverslip stained slides both manually and by using automated staining. * Perform and document routine preventative maintenance on automated equipment. * Identify normal basic organs (lungs, heart, liver, spleen, kidney) stained with H&E microscopically. * File paraffin blocks, stained slides and fixed tissue according. * Package, label and ship pathology specimens. * Handle and dispose hazardous chemicals and biological material according to laboratory safety policy. * Use document control system for updating and implementing controlled documents. * Accession and enter data regarding specimens using department accessioning database. * Perform all other related duties as assigned. Qualifications: * Education: Associate's degree (A.A./A.S.) in biological science required. Bachelor's degree (B.A./B.S.) preferred. * Experience: 0 to 1 year. * Certification/Licensure: None. * Other: None. Equal Employment Opportunity: Charles River Laboratories is an equal opportunity employer who values diversity in the workplace. Kathy Cormier Histology Manager Charles River Laboratories Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. From wecare <@t> qualityhistology.com Tue Aug 9 09:30:01 2011 From: wecare <@t> qualityhistology.com (wecare@qualityhistology.com) Date: Tue Aug 9 09:30:16 2011 Subject: [Histonet] Medite Equipment & Histo Pro 3030 Robotic Slide Stainer Message-ID: <00e601cc56a0$d34544b0$79cfce10$@qualityhistology.com> Please note that HistoPro? 3030 Robotic Slide Stainer is designed and manufacture in USA by RUSHABH Instruments in Pennsylvania. You can contact RUSHABH Instruments at 215-491-0081 for support. Thanks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON Sent: Monday, August 08, 2011 9:19 PM To: Histonet Subject: [Histonet] Medite Equipment Does anyone?have an opinion good or bad on?Medite Equipment??Specifically a TPC 15 Tissue Processor or Histo Pro 3030 Robotic Slide Stainer. And if?so, who does the servicing on the equipment. ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1391 / Virus Database: 1520/3822 - Release Date: 08/08/11 From nicole <@t> dlcjax.com Tue Aug 9 09:49:35 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Aug 9 09:49:45 2011 Subject: [Histonet] Job Opening KC, Missouri Message-ID: <2077.208.62.167.196.1312901375.squirrel@webmail.realpages.com> Busy Derm practice looking for an independant, hard working histotech, for a dermatopathology lab. Must be proficient in all aspect of routine histology. Grossing, staining, accessioning, mircotonomy. Full time position. Please call Tammi for immediate consideration. (816)584-8100 From ploykasek <@t> phenopath.com Tue Aug 9 10:25:11 2011 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Aug 9 10:25:17 2011 Subject: [Histonet] position opening Message-ID: <7CEF45E1-3EA8-4D24-B7D9-423803826FAC@phenopath.com> Due to an upcoming retirement, PhenoPath has a position opening in the contract research department. This position is as described below. I'm happy to answer any questions on this position. Contract Research Laboratory Supervisor PhenoPath Laboratories, PLLC, has an opportunity for a Laboratory Supervisor in its Contract Research Department. This is a supervisory position located in Seattle, WA and reports to the Director of Contract Research. Primary Responsibilities Responsibilities include, and are not limited to: Project Development ? managing all aspects of client requests for R&D projects to include project proposals, reporting project status, and resolution of questions; Project Execution ? oversee and coordinate work of Research Technologists to include assay development, specimen tracking and testing, and quality review of slides; Supervision of Contract Research personnel ? staff training and development, performance management, assist in recruiting efforts for new staff. Required Skills/Experience Requires 8-10 years of Immunohistochemistry experience and 3-5 years of supervisory and project management experience; preference for an HTL or an MT with experience working in a clinical laboratory or GLP laboratory setting. TO APPLY, PLEASE SEND COVER LETTER AND RESUME TO: PhenoPath Laboratories, PLLC 551 N. 34th St., Suite 100 Seattle, WA 98103 Phone: 206 374-9000 Fax: 206 374-9009 E-mail: jobs@phenopath.com Patti Loykasek PhenoPath Laboratories Clinical Lab Manager Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From carrieleighd <@t> yahoo.com Tue Aug 9 10:30:42 2011 From: carrieleighd <@t> yahoo.com (Carrie Dula) Date: Tue Aug 9 10:30:55 2011 Subject: [Histonet] Re: Knife for trimming paraffin Message-ID: <1312903842.74894.YahooMailClassic@web65509.mail.ac4.yahoo.com> I currently use a paratrimmer. However, in the past I used an extremely dulled metal scalpel with a non-disposable scalpel. I dulled it by scraping it on concrete then on cardboard boxes. It is perfect for popping blocks out of the molds and also the perfect size for quickly scraping the sides of the blocks. I find that it is a lot easier and safer to use than the paring knife that many histotechs use. Carrie L Dula, HTL, SLS(ASCP) --- On Tue, 8/9/11, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 93, Issue 11 To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 9, 2011, 10:25 AM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Re: Re:? Knife for trimming paraffin from blocks ? ? ? (histotech@imagesbyhopper.com) ???2. RE: Histonet Digest, Vol 93, Issue 9 (Joanne Clark) ???3. : [Histonet] re-cycled xylene in tissue processor (White, Lisa M.) ---------------------------------------------------------------------- Message: 1 Date: Mon, 08 Aug 2011 11:46:55 -0400 From: "histotech@imagesbyhopper.com" Subject: Re: [Histonet] Re:? Knife for trimming paraffin from blocks To: John Shelley Cc: "histonet@lists.utsouthwestern.edu" ??? ,??? "Cormier, Kathleen" ??? Message-ID: <7AC5A3C6-0AAD-434A-962D-5F44B523EBE9@imagesbyhopper.com> Content-Type: text/plain; charset=us-ascii I agree, the Para-Trimmer is the next best thing since sliced bread!? I'll never go back to the knife again. We use lid from one of the Ventana special stains boxes to catch the wax.? I have also fashioned a drip tray out of the cardboard boxes that the coverslips come in. We're all McGuyver's at heart, aren't we?? :o) Sent from my iPhone On Aug 8, 2011, at 11:14 AM, John Shelley wrote: > Hi All, > > I likewise use the Para-trimmer and instead of a specimen cup I just use the top lid of a 1/2 gross microscope slide box. Once filled I just throw away in the trash. > > Kind Regards! >? > John J Shelley > Senior Research Associate, Histology Core > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cormier, Kathleen > Sent: Monday, August 08, 2011 11:04 AM > To: gayle callis; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Knife for trimming paraffin from blocks > > We use the paratrimmer too. What we do for a paraffin catcher is to use > a specimen cup (I call them urine cups, but whatever) to catch the > paraffin. When it get fullish, we place in the slide oven to melt the > paraffin, when melted pour off, and reuse the cup... :) > > Kathy Cormier > Histology Manager > Charles River Laboratories > 251 Ballardvale Street > Wilmington, MA 01887 > Ph: 781-222-6803 > Fax: 978-988-8793 > kathleen.cormier@crl.com > Accelerating Drug Development. Exactly. > Notice - This email and any files transmitted with it are confidential > and may contain privileged and/or proprietary information. You must not > disclose this message to another party without Charles River's express > written consent. If you are not the intended recipient you must not > copy, distribute or use this email or the information contained in it > for any purpose other than to notify us. If you have received this > message in error, please notify Charles River immediately, and delete it > from your system. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle > callis > Sent: Monday, August 08, 2011 10:56 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Knife for trimming paraffin from blocks > > You Wrote: > > > > I am looking for a stainless steel knife that we use to scrape off the > > paraffin from the embedded blocks.? I could not find the 6 inches size > > knife in any catalog. > > > > Thanks in advance > > > > Mala > > > > Nirmala Srishan > > Histology Supervisor > > Holy Name Medical Center. > > > > ************************************************************************ > * > > Dear Mala, > > > > In the past, we used an old style permanent edge scalpel blade but after > a > disaster with one person seirously cutting himself while trimming > paraffin > from a block, we purchased a Paratrimmer with a heated, slanted metal > surface.? This trimmer has made everyone happy by eliminating the > potential > for serious injury and does an even better job of getting rid of excess > paraffin.? ??? > > > > There are two models sold, one from Thermo Scientific and the other one > was > recently spotted on a vendor website. (Sorry, I didn't jot the name > down). > The trimmers? are worth the investment to keep you and your employees > safe > from nasty cuts, no matter what the knife/blade, etc could be used.? > > > > The only drawback to the Para Trimmer is the messy paraffin drippings > have > to be collected in some separate container that the manufacturer doesn't > supply.? This is more than a bit annoying.? It seems to me the > manufacturer > of these devices would design the trimmer with a paraffin catch tray.? A > small aluminum baking pan (from grocery store) could be used and > disposed of > (not a "Green" consideration).? ? > > > > Be safe rather than sorry.? > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 2 Date: Mon, 8 Aug 2011 16:01:19 +0000 From: Joanne Clark Subject: [Histonet] RE: Histonet Digest, Vol 93, Issue 9 To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <0494A7D4E8CC254EA2FB81464982E3786EA635@S10MAILD001N1.SH10.lan> Content-Type: text/plain; charset="us-ascii" We have been using recycled xylene on our VIP5 for the past 5 years and haven't had any problems.? We use it for both the cleaning cycles and the xylene stations. Joanne Clark, HT Histology Supervisor PCNM ------------------------------ Message: 9 Date: Mon, 8 Aug 2011 09:33:43 -0500 From: "Gaiser, Marcia" Subject: [Histonet] re-cycled xylene in tissue processor To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <728F817C02110E498D803A7C3B0C6248068D43B5E0@S009-APEXM06.ds.ad.ssmhc.com> ??? Content-Type: text/plain;??? charset="iso-8859-1" Hi, Has anyone had experience using re-cycled xylene in the Tissue-Tek VIP5 tissue processor for the clean cycle? Will use of re-cycled xylene, over time, damage the processor? Thank you, Marcia Gaiser Pathology Supervisor Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ------------------------------ ------------------------------ Message: 3 Date: Mon, 8 Aug 2011 12:10:34 -0400 From: "White, Lisa M." Subject: : [Histonet] re-cycled xylene in tissue processor To: Message-ID: ??? <2B2ECF33934F5D4996D8BE03EFDF39760874200A@VHAV09MSGA3.v09.med.va.gov> Content-Type: text/plain; charset="us-ascii" We have recycled xylene in the past and now Formula 83 and have a VIP 5 and VIP 6.? Sakura told us when we purchased each processor NOT to use recycled solvent in Sakura processors for the cleaning solvent station. If you want to give it a try would suggest waiting until the warranty has expired on the off chance that something would go wrong. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 93, Issue 11 **************************************** From lpjones <@t> srhs-pa.org Tue Aug 9 10:39:22 2011 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Tue Aug 9 10:39:27 2011 Subject: [Histonet] Old Green Staining Racks Message-ID: <4AE8039AEA096143B965CBC6D092166802351B394B@EXCH2007.srhs-pa.org> Does anyone remember the old green staining racks that had an inverted "L" metal handle and held 25 slides? We are trying to find somewhere to order them, but having no luck. Any suggestions are welcome! And, thanks in advance! The Histochicks at Sharon Regional ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From cpyse <@t> x-celllab.com Tue Aug 9 10:55:37 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Aug 9 10:55:49 2011 Subject: [Histonet] Old Green Staining Racks In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B394B@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D092166802351B394B@EXCH2007.srhs-pa.org> Message-ID: <001b01cc56ac$c83657b0$58a30710$@com> Market Lab cat#ML1212. I would check the cat# before ordering, the picture in the catalog show the right rack but the description is different. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Tuesday, August 09, 2011 11:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Green Staining Racks Does anyone remember the old green staining racks that had an inverted "L" metal handle and held 25 slides? We are trying to find somewhere to order them, but having no luck. Any suggestions are welcome! And, thanks in advance! The Histochicks at Sharon Regional ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Amanda.Steier <@t> sanfordhealth.org Tue Aug 9 10:59:34 2011 From: Amanda.Steier <@t> sanfordhealth.org (Steier,Amanda) Date: Tue Aug 9 10:59:39 2011 Subject: [Histonet] Napsin-A Message-ID: I am starting to work up Napsin-A (purchased from Biocare Medical) on our Ventana instruments using iView DAB. Does anyone have a recommended protocol to get me started? Amanda Steier, HT(ASCP) Sanford Health Fargo, ND ----------------------------------------------------------------------- Please note that My Email Address Has Changed! Please begin using the address in the "From" line above, immediately. Soon, email sent to my old address will no longer be delivered to me. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Ronald.Houston <@t> nationwidechildrens.org Tue Aug 9 11:01:48 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Aug 9 11:02:01 2011 Subject: [Histonet] TFE-3 Message-ID: Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From settembr <@t> umdnj.edu Tue Aug 9 11:04:50 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Tue Aug 9 11:05:02 2011 Subject: [Histonet] RE: TFE-3 In-Reply-To: References: Message-ID: We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958 Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, August 09, 2011 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] TFE-3 Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Tue Aug 9 11:21:41 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Aug 9 11:23:29 2011 Subject: [Histonet] RE: Old Green Staining Racks In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B394B@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D092166802351B394B@EXCH2007.srhs-pa.org> Message-ID: Mercedes Medical MER1040 - Slide Staining Dipper, with metal handle - took me awhile to find them too. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Tuesday, August 09, 2011 9:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Green Staining Racks Does anyone remember the old green staining racks that had an inverted "L" metal handle and held 25 slides? We are trying to find somewhere to order them, but having no luck. Any suggestions are welcome! And, thanks in advance! The Histochicks at Sharon Regional ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Aug 9 13:05:51 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Aug 9 13:05:57 2011 Subject: [Histonet] RE: TFE-3 In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E12D90D@evcspmbx3.ads.northwestern.edu> Same here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, August 09, 2011 11:05 AM To: 'Houston, Ronald'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: TFE-3 We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958 Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, August 09, 2011 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] TFE-3 Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Tue Aug 9 14:09:47 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Aug 9 14:09:58 2011 Subject: [Histonet] What's your favorite phospho-histone H3 antibody for IHC? Message-ID: ....p(Ser10) or p(Ser28) ? And have you found pHH3 (Ser28) to be more M-phase specific? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From collette2 <@t> llnl.gov Tue Aug 9 14:10:24 2011 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Tue Aug 9 14:10:36 2011 Subject: [Histonet] Manual embedding In-Reply-To: Message-ID: Hi, All, I just saw this question and the responses, thought I would add my own solution to the mix. I do manual embedding, I use a hyb oven for my infiltrating/embedding station (like for hybridizing Southern or Northern blots- who does that anymore?). I have taken out the rotating wheel for spinning bottles, and line the tray at the bottom with foil. Temp control works very well. I have several Wheaton staining boxes (the kind that come with glass inserts for staining 20 slides) that I use for my wax changes for infiltrating, and a couple of metal beakers I use for pouring into molds. I have a little real estate left inside the oven (it's probably around 18"x 18" square area) to heat my molds for pouring, and I have a metal heat block in there that you would use for Eppendorf tubes as my forceps warmer. It works pretty well, but does take a long time to heat up those boxes of wax, so I need to plan ahead by about 3 hours. When I pour the molds, I have the oven in front of a drawer under the bench, I line an extra cabinet shelf with foil and lay it over the open drawer to give me some bench space, pour my molds inside the oven and transfer it to the foil-lined shelf to cool so I can move it without the specimen shifting. Then I transfer to a photo tray and cool in the fridge for a couple of hours before releasing the molds. With the door of the hyb oven open I have to embed in shifts and let the molds heat up again, but it works OK. At least it's all contained in one unit. It's hard to do histology in a molecular lab ;) Hope this helps to give a do-it-your-selfer some ideas. Sincerely, Nicole Collette LLNL On 8/2/11 7:53 PM, "Scott Parker" wrote: > Dear Histonetters: > > I am interested in acquiring a pitcher and heating jacket for melting and > pouring paraffin during manual embedding. My work is relatively low volume > and in a university research lab setting so I am trying to avoid purchasing > an expensive embedding station. Can anyone recommend an honest supplier of > used histology equipment that might be able to provide me with this item? > > Thank you for your expertise! > > Scott L. Parker > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From angela-cordle <@t> uiowa.edu Tue Aug 9 15:46:03 2011 From: angela-cordle <@t> uiowa.edu (Cordle, Angela R) Date: Tue Aug 9 15:46:10 2011 Subject: [Histonet] Fern tissue loss during in situ hybridization Message-ID: <32F6F3C172009D44A3B5E6702B54DBF602A772D2@itsnt443.iowa.uiowa.edu> Dear Histonet members, I?m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I?m not an expert at this process, but I?ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt?s coated slies), and I?ve had success with Arabidpsis ISH. I?ll outline the relevant and troubleshooting procedures that I?ve gone through here: 1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides. 2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides. 3. For mounting, I float tissue on 37?C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures. 4. I?ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48?C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results. 5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature. 5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH. Okay. So, here are my specific questions: Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide? What the beep should I try next? In advance, thank you all so much for taking the time to help me out with this frustrating problem! --Angie From rjbuesa <@t> yahoo.com Tue Aug 9 15:59:18 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 9 15:59:26 2011 Subject: [Histonet] Fern tissue loss during in situ hybridization In-Reply-To: <32F6F3C172009D44A3B5E6702B54DBF602A772D2@itsnt443.iowa.uiowa.edu> Message-ID: <1312923558.59874.YahooMailClassic@web65711.mail.ac4.yahoo.com> I think you should try to find out about the cellulose contents of the fern you are working with, and compare it to that of Arabidopsis. If the fern (Ceratopteris) contains more cellulose, I think this is the cause of your problem. If that is the case I think you should try to use a paraffin wax of higher melting point (63-65?C) to assure an infiltration more compatible with the cellulose. Also make sure that the infiltration is optimal. Finally if you can use thinner sections (<7 ?m) that could help also. Ren? J. --- On Tue, 8/9/11, Cordle, Angela R wrote: From: Cordle, Angela R Subject: [Histonet] Fern tissue loss during in situ hybridization To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, August 9, 2011, 4:46 PM Dear Histonet members, I?m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I?m not an expert at this process, but I?ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt?s coated slies), and I?ve had success with Arabidpsis ISH. I?ll outline the relevant and troubleshooting procedures that I?ve gone through here: 1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides. 2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides. 3. For mounting, I float tissue on 37?C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures. 4. I?ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48?C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results. 5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature. 5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH. Okay. So, here are my specific questions: Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide? What the beep should I try next? In advance, thank you all so much for taking the time to help me out with this frustrating problem!? ? --Angie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihisto <@t> hotmail.com Tue Aug 9 19:46:25 2011 From: brandihisto <@t> hotmail.com (Brandi Farris) Date: Tue Aug 9 19:46:29 2011 Subject: [Histonet] Sterilized cassette Message-ID: My OR department is wanting a small biopsy cassette that they can sterilize, be in the sterile field and place small biopsy specimens directly into a cassette before placing in formalin. We've had a problem with small biopsies dissolving/disappearing in telfa pads. We can't seem to find a cassette that the manufacture approves to be sterilized. I have a sample of a metal biopsy cassette, but the lid closure is questionable. I'm having trouble thinking "out of the box" on this problem. I've never had an OR request this before. Any tips would be greatly appreciated! Thank you, Brandi Capital Region Medical Center Jefferson City, MO From lynn13361 <@t> aol.com Wed Aug 10 02:58:46 2011 From: lynn13361 <@t> aol.com (lynn13361@aol.com) Date: Wed Aug 10 02:58:55 2011 Subject: [Histonet] Re:2 Message-ID: <8CE254A777897B7-15C0-2CD98@Webmail-d108.sysops.aol.com> Hello! Tell me your feelings after trying this stuff!. http://aldeiadovale.com.br/com.page.php?pyhID=86tj5 From mpence <@t> grhs.net Wed Aug 10 07:57:27 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Aug 10 07:57:32 2011 Subject: [Histonet] Sterilized cassette In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974C58@is-e2k3.grhs.net> My question is this. Who will open the cassette and dictate the specimen and what if the specimen does not make it thru processing? Who is responsible then for it missing? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Farris Sent: Tuesday, August 09, 2011 7:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterilized cassette My OR department is wanting a small biopsy cassette that they can sterilize, be in the sterile field and place small biopsy specimens directly into a cassette before placing in formalin. We've had a problem with small biopsies dissolving/disappearing in telfa pads. We can't seem to find a cassette that the manufacture approves to be sterilized. I have a sample of a metal biopsy cassette, but the lid closure is questionable. I'm having trouble thinking "out of the box" on this problem. I've never had an OR request this before. Any tips would be greatly appreciated! Thank you, Brandi Capital Region Medical Center Jefferson City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> wvhcs.org Wed Aug 10 09:35:01 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Wed Aug 10 09:35:05 2011 Subject: [Histonet] Cell block H&E staining Message-ID: Hello all, I'm interested to see what staining protocol folks are using for H&E staining of cell blocks. Lately we've been getting varying light and dark staining on cell blocks only. Currently they are run on our standard H&E protocol that we use for all tissues. Does anyone use a separate protocol for cell blocks? Thanks! Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From rjbuesa <@t> yahoo.com Wed Aug 10 09:38:17 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 10 09:38:22 2011 Subject: [Histonet] Cell block H&E staining In-Reply-To: Message-ID: <1312987097.19039.YahooMailClassic@web65707.mail.ac4.yahoo.com> The? staining variability you are obtaining on cell blocks is caused by the medium you are preparing them in. We always used the same H&E protocol and I do not think you will solve the problems by changing the H&E protocol, but by modifying the way you prepare the cell block. Ren? J. --- On Wed, 8/10/11, Dessoye, Michael J wrote: From: Dessoye, Michael J Subject: [Histonet] Cell block H&E staining To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 10, 2011, 10:35 AM Hello all, I'm interested to see what staining protocol folks are using for H&E staining of cell blocks.? Lately we've been getting varying light and dark staining on cell blocks only.? Currently they are run on our standard H&E protocol that we use for all tissues.? Does anyone use a separate protocol for cell blocks? Thanks! Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Aug 10 10:02:18 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Aug 10 10:02:15 2011 Subject: [Histonet] Histotech needed in San Diego. Can you help? Message-ID: <10BE35346E43450B80E74E70B6469EBE@ownerf1abaad51> Hi Histonetters!! How are you? I have a new histology position and I need your help. I am currently working with a leading edge cancer diagnostic laboratory in the San Diego area that is in need of a histotechnologist with strong immunohistochemistry experience. This is a permanent full time position and the schedule is 9a-530p Tuesday-Saturday. The client offers a great environment, a great crew to work with, excellent salary, great benefits and a generous relocation package. ASCP HT or HTL and a B.S. degree in a science related major are required. QIHC is a plus. My question is do you know of anyone who might be interested in this position? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 500.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From rmweber113 <@t> comcast.net Wed Aug 10 10:47:29 2011 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Wed Aug 10 10:47:31 2011 Subject: [Histonet] CYTOLOGIST JOB OPENING PHILLY In-Reply-To: <1613288220.17543.1312990995514.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: <1743836975.17768.1312991249090.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> We have a part time job evening?opening for a cytologist to read urine cytology.? The job is located in the Northeast Philadelphia area in a urology practice.? Eligible candidates? must have at least 3 years experience.? Candidates can fax resumes to 215 947-2015 attention laboratory or call 215 947-4480. Thank you, From sbaldwin <@t> mhhcc.org Wed Aug 10 10:47:42 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Aug 10 10:47:46 2011 Subject: [Histonet] GROSSING SPECIMENS Message-ID: Histonetters If my Histo tech has an associates degree with enough chemistry courses can they gross the small specimens?? Acording to the new CAP, JOINT COMMISSION nad CLIAA regs?? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Aug 10 10:56:33 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Aug 10 10:56:38 2011 Subject: [Histonet] Cyrostat Oppinions Message-ID: Hi We are in the process of getting a new cyrostat and would like some opinions. We are looking at the ThermoFisher HM550. Please feel free to contact me offline if you like. Thank you in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From katelin09htl <@t> gmail.com Wed Aug 10 14:27:48 2011 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Wed Aug 10 14:27:52 2011 Subject: [Histonet] GROSSING SPECIMENS In-Reply-To: References: Message-ID: CLIA requirements are very clear for any testing personnel doing the grossing. Be sure you read the CLIA law regarding grossing requirements and be sure all of your grossing personnel not only meet these requirements but that their qualifications are well documented-including updated resumes indicating that they are CLIA eligible to gross. All grossing is a high complexity task. I would highly recommend you review the CLIA website for yourself and if you have any questions, call your local CLIA office. I just went through my CLIA inspection two weeks ago, feel free to contact me if you have any other questions. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 TESTING PERSONNEL 1. Licensed MD, DO or DPM. 2. Doctorate, master's, or bachelor's in laboratory science. 3. Education and training equivalent to an associate degree in a laboratory science or medical laboratory technology that includes at least 60 semester hours including 24 semester hrs of medical lab technology and at least 3 months training in each specialty in which high complexity testing is performed; or, 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: completion of a clinical lab training program and at least 3 months training in each specialty in which high complexity testing is performed. On Wed, Aug 10, 2011 at 8:47 AM, Sara Baldwin/mhhcc.org wrote: > Histonetters > If my Histo tech has an associates degree with enough chemistry courses can > they gross the small specimens?? Acording to the new CAP, JOINT COMMISSION > nad CLIAA regs?? > > Thanks > Pathology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-482-0210, 0216, Fax 812-482-0232, > Pager 812-481-0897, Cell 812-887-3357 > Confidential information, Authorized use only. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PMonfils <@t> Lifespan.org Wed Aug 10 14:46:01 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Aug 10 14:46:12 2011 Subject: [Histonet] Thick Paraffin Sections of Rat Brain? Message-ID: <4EBFF65383B74D49995298C4976D1D5E087F0183@LSRIEXCH1.lsmaster.lifespan.org> Does anyone cut 40 micron paraffin sections of rat brain? Do you have any problem with fine parallel cracks in the sections (parallel to the knife edge)? Any suggestions for avoiding this? Thanks. From b427297 <@t> aol.com Wed Aug 10 16:30:21 2011 From: b427297 <@t> aol.com (b427297@aol.com) Date: Wed Aug 10 16:30:30 2011 Subject: [Histonet] Shipping prepared histology slides Message-ID: <8CE25BBD7A6BE8D-25B4-489@webmail-m153.sysops.aol.com> Can someone provide a quick reference to ANYTHING I can use to prove to my EHS that fixed, processed, coverslipped slides are not HAZMAT? They insist shipping finished slides cannot be performed by histotechs. Wha? Thanks. From wenyizhang72 <@t> yahoo.com Wed Aug 10 16:44:48 2011 From: wenyizhang72 <@t> yahoo.com (wenyi zhang) Date: Wed Aug 10 16:44:51 2011 Subject: [Histonet] will reembed frozen tissue in OCT affect the histology Message-ID: <1313012688.14975.YahooMailClassic@web162015.mail.bf1.yahoo.com> I have some old frozen tissue block embedded in OCT and kept in -80. When I used cryostat to cut the sections, I found the block hard and brittle, the tissue inside block is coiled or look like a network. Is something wrong with the embedding? Can I reembed the tissue in new OCT? Thanks for any reply! From AnthonyH <@t> chw.edu.au Wed Aug 10 18:30:43 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Aug 10 18:30:51 2011 Subject: [Histonet] will reembed frozen tissue in OCT affect the histology In-Reply-To: <1313012688.14975.YahooMailClassic@web162015.mail.bf1.yahoo.com> References: <1313012688.14975.YahooMailClassic@web162015.mail.bf1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157188990E8@xmdb02.nch.kids> It is possible that the tissue has "freeze-dried". If you melt the specimen and re-embed in OCT you might find severe drying artefact, rendering poor morphology (though most enzymes would not be affected). I would advise you to look at a recent article in Journal of Histotechnology that describes a new method to transport muscle biopsies in Aedesta(tm)-cell/tissue preservation (ACTP) media. The authors also found that the immersion in ACTP of previously frozen muscle with extensive artefacts significantly reduced ice crystal artefacts and restored original sarcoplasmic morphology and enzymatic activity (Horkayne-Szakaly et al (2009) "Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic Activity and Morphology" J Histotechnol 32(2):49-53) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wenyi zhang Sent: Thursday, 11 August 2011 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] will reembed frozen tissue in OCT affect the histology I have some old frozen tissue block embedded in OCT and kept in -80. When I used cryostat to cut the sections, I found the block hard and brittle, the tissue inside block is coiled or look like a network. Is something wrong with the embedding? Can I reembed the tissue in new OCT? Thanks for any reply! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From histotech <@t> imagesbyhopper.com Wed Aug 10 18:52:40 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Aug 10 18:52:52 2011 Subject: [Histonet] Shipping prepared histology slides In-Reply-To: <8CE25BBD7A6BE8D-25B4-489@webmail-m153.sysops.aol.com> References: <8CE25BBD7A6BE8D-25B4-489@webmail-m153.sysops.aol.com> Message-ID: <6A7D9191-C539-4D93-9A8E-D40F0A4B38A9@imagesbyhopper.com> Good gosh, don't tell that to the reference labs who routinely send out finished slides!! Sent from my iPhone On Aug 10, 2011, at 5:30 PM, b427297@aol.com wrote: > > Can someone provide a quick reference to ANYTHING I can use to prove to my EHS that fixed, processed, coverslipped slides are not HAZMAT? > They insist shipping finished slides cannot be performed by histotechs. Wha? > Thanks. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rachael.Glebocki <@t> nottingham.ac.uk Thu Aug 11 03:10:23 2011 From: Rachael.Glebocki <@t> nottingham.ac.uk (Rachael Glebocki) Date: Thu Aug 11 03:19:13 2011 Subject: [Histonet] Decalcification of bone Message-ID: <0365FF1B8A3FD844A27C1BF96C1871BD361B5653CF@EXCHANGE1.ad.nottingham.ac.uk> Dear Histonet users, I was wondering if anyone has a operation procedure for bone decalcification that works. I am having no joy in decalcifying the bone and making a good slide from it. Thank you for your time. Rachael Glebocki Teaching Technician School of Veterinary Medicine & Science University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. From anonwums1 <@t> gmail.com Thu Aug 11 06:26:44 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Aug 11 06:26:49 2011 Subject: [Histonet] Decalcification of bone In-Reply-To: <0365FF1B8A3FD844A27C1BF96C1871BD361B5653CF@EXCHANGE1.ad.nottingham.ac.uk> References: <0365FF1B8A3FD844A27C1BF96C1871BD361B5653CF@EXCHANGE1.ad.nottingham.ac.uk> Message-ID: Hi Rachael, I work with mouse bones on a regular basis, and I assure you that they are incredibly difficult to work with, but with practice you can get decent sections. It would be useful if you gave us a bit more information. What kind of bones are you decalcifying? How are they fixed? How are you currently decalcifying? Are you paraffin embedding or cutting frozen sections? What is exactly happening when you're cutting the bones that gives you poor quality? What is your end goal (IHC, IF, H&E)? Adam On Thu, Aug 11, 2011 at 3:10 AM, Rachael Glebocki < Rachael.Glebocki@nottingham.ac.uk> wrote: > Dear Histonet users, > > I was wondering if anyone has a operation procedure for bone > decalcification that works. I am having no joy in decalcifying the bone and > making a good slide from it. > > Thank you for your time. > > Rachael Glebocki > Teaching Technician > School of Veterinary Medicine & Science > University of Nottingham > > This message and any attachment are intended solely for the addressee and > may contain confidential information. If you have received this message in > error, please send it back to me, and immediately delete it. Please do not > use, copy or disclose the information contained in this message or in any > attachment. Any views or opinions expressed by the author of this email do > not necessarily reflect the views of the University of Nottingham. > > This message has been checked for viruses but the contents of an attachment > may still contain software viruses which could damage your computer system: > you are advised to perform your own checks. Email communications with the > University of Nottingham may be monitored as permitted by UK > legislation._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jjcampbell14 <@t> att.net Thu Aug 11 10:49:01 2011 From: jjcampbell14 <@t> att.net (John & Jen Campbell) Date: Thu Aug 11 10:49:05 2011 Subject: [Histonet] potassium hydroxide/reprocessing for nails Message-ID: <417981.92892.qm@smtp107-mob.biz.mail.bf1.yahoo.com> Hi all, I was told to use potassium hydroxide to soften an already processed nail I am removing the paraffin from the tissue right now (xylene, followed by EtOH). My questions are: 1)How long should the nail remain in KOH-test it periodically? 2) If I do not plan on reprocessing the tissue til tomorrow, can the nail remain in KOH overnight or shld I transfer it to a diff solution, and if so, what would that be. 3) What times in each of the graded alcohols, xylenes and paraffin do u recommend for hand-reprocessing. I've done this before but do not recall the times for each. Thank you so much in advance! Jennifer From rjbuesa <@t> yahoo.com Thu Aug 11 10:56:34 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 11 10:56:37 2011 Subject: [Histonet] Decalcification of bone In-Reply-To: <0365FF1B8A3FD844A27C1BF96C1871BD361B5653CF@EXCHANGE1.ad.nottingham.ac.uk> Message-ID: <1313078194.7015.YahooMailClassic@web65714.mail.ac4.yahoo.com> As a matter of fact any decalciying technique presented in any histotechnique book works. The differences reside in the time it will take and the decalcifying method you use. I will be very complicated to indicate you one, but a commercial productuct called "RDO" is one I used for hard bones and EDTA solution for bone marrow biopsies. I this you will better of in consulting a techniques book and follow the instructions, especially those related to the "end point" of the decalcification. Ren? J. --- On Thu, 8/11/11, Rachael Glebocki wrote: From: Rachael Glebocki Subject: [Histonet] Decalcification of bone To: "histonet@lists.utsouthwestern.edu" Date: Thursday, August 11, 2011, 4:10 AM Dear Histonet users, I was wondering if anyone has a operation procedure for bone decalcification that works. I am having no joy in decalcifying the bone and making a good slide from it. Thank you for your time. Rachael Glebocki Teaching Technician School of Veterinary Medicine & Science University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it.???Please do not use, copy or disclose the information contained in this message or in any attachment.? Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 11 10:59:22 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 11 10:59:25 2011 Subject: [Histonet] potassium hydroxide/reprocessing for nails In-Reply-To: <417981.92892.qm@smtp107-mob.biz.mail.bf1.yahoo.com> Message-ID: <1313078362.53260.YahooMailClassic@web65702.mail.ac4.yahoo.com> The nails should remain in 10% aq NaOH (or KOH)?for half an hour at the most. Leaving them overnight will macerate them. After that process as usual.Ren? J. --- On Thu, 8/11/11, John & Jen Campbell wrote: From: John & Jen Campbell Subject: [Histonet] potassium hydroxide/reprocessing for nails To: "histonet@lists.utsouthwestern.edu" Date: Thursday, August 11, 2011, 11:49 AM Hi all, ? I was told to use potassium hydroxide to soften an already processed nail I am removing the paraffin from the tissue right now (xylene, followed by EtOH). My questions are: 1)How long should the nail remain in KOH-test it periodically? 2) If I do not plan on reprocessing the tissue til tomorrow, can the nail remain in KOH overnight or shld I transfer it to a diff solution, and if so, what would that be. 3)? What times in each of the graded alcohols, xylenes and paraffin do u recommend for hand-reprocessing. I've done this before but do not recall the times for each. Thank you so much in advance! Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jjcampbell14 <@t> att.net Thu Aug 11 11:08:40 2011 From: jjcampbell14 <@t> att.net (John & Jen Campbell) Date: Thu Aug 11 11:08:43 2011 Subject: [Histonet] potassium hydroxide/reprocessing of nail Message-ID: <1313078920.46934.YahooMailClassic@web180208.mail.gq1.yahoo.com> Hi All, I was asked to place an already processed and embedded nail in KOH to soften it so the pathologist can section it further. Right now the nail is going through the changes of xylene to remove paraffin, followed by changes of alcohol. My questions are: 1) For how long do you typically leave the nail in KOH? Chec on it periodically I'm assuming? 2) Is it safe to leave the nail in KOH overnight (I will not be able to reprocess until tomorrow) or should it be moved to another solution, and if so, what would that be? 3) What schedule do you recommend for hand processing the tissue? I've done this in the past but do not recall the times and unfortunately do not have access to that info where I am at the moment. Thank you so much in advance! Have a great day! Jennifer From rjbuesa <@t> yahoo.com Thu Aug 11 11:15:40 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 11 11:15:44 2011 Subject: [Histonet] potassium hydroxide/reprocessing of nail In-Reply-To: <1313078920.46934.YahooMailClassic@web180208.mail.gq1.yahoo.com> Message-ID: <1313079340.76991.YahooMailClassic@web65713.mail.ac4.yahoo.com> Half an hour in 10% aq. KOH or NaOH, no more. In 70% Ethol after that. Never overnight. Ren? J. --- On Thu, 8/11/11, John & Jen Campbell wrote: From: John & Jen Campbell Subject: [Histonet] potassium hydroxide/reprocessing of nail To: histonet@lists.utsouthwestern.edu Date: Thursday, August 11, 2011, 12:08 PM Hi All, ? I was asked to place an already processed and embedded nail in KOH to soften it so the pathologist can section it further.? Right now the nail is going through the changes of xylene to remove paraffin, followed by changes of alcohol.? My questions are: 1) For how long do you typically leave the nail in KOH? Chec on it periodically I'm assuming? 2) Is it safe to leave the nail in KOH overnight (I will not be able to reprocess until tomorrow) or should it be moved to another solution, and if so, what would that be? 3) What schedule do you recommend for hand processing the tissue? I've done this in the past but do not recall the times and unfortunately do not have access to that info where I am at the moment. Thank you so much in advance! Have a great day! Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Aug 11 11:18:45 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Aug 11 11:18:50 2011 Subject: [Histonet] potassium hydroxide/reprocessing of nail In-Reply-To: <1313078920.46934.YahooMailClassic@web180208.mail.gq1.yahoo.com> References: <1313078920.46934.YahooMailClassic@web180208.mail.gq1.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408277BDA2F@CHEXCMS10.one.ads.che.org> I would not reprocess it as that will make it even cripser. I would face the as gently as possible so as not to pop it out and soak the block in soap or Nair... My 2 cents.. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John & Jen Campbell Sent: Thursday, August 11, 2011 12:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] potassium hydroxide/reprocessing of nail Hi All, I was asked to place an already processed and embedded nail in KOH to soften it so the pathologist can section it further. Right now the nail is going through the changes of xylene to remove paraffin, followed by changes of alcohol. My questions are: 1) For how long do you typically leave the nail in KOH? Chec on it periodically I'm assuming? 2) Is it safe to leave the nail in KOH overnight (I will not be able to reprocess until tomorrow) or should it be moved to another solution, and if so, what would that be? 3) What schedule do you recommend for hand processing the tissue? I've done this in the past but do not recall the times and unfortunately do not have access to that info where I am at the moment. Thank you so much in advance! Have a great day! Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Clough <@t> medicine.tamhsc.edu Thu Aug 11 11:37:06 2011 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Thu Aug 11 11:37:12 2011 Subject: [Histonet] Needing advice on mice calvarial paraffin embedding and sectioning. Message-ID: Hello Everyone , I am writing you because I need advice on paraffin embedding and sectioning of mice calvarials. I?ve processed manually a calvarial using the following protocol and had problems with the tissue tearing and or the paraffin detaching (pulling away ) from border with the tissue creating a hole /tear in the paraffin section. This tissue was processed the follow method: 1.Tissue was fixed in 10% NBF for a couple of days, then placed in Carson?s fix until needed. 2.Tissue washed in 1%PBS x2, placed in 1M EDTA for 7 days to decalcify. 3. Wash tissue in 1%PBS x2, place in 30% ETOH for 30 min at RT. 4. Remove from 30%ETOH and place in 50% ETOH for 30 min at RT. 5. Remove from 50%ETOH and place in 70% ETOH store in 4C refrigerator until available to continue processing (a couple of days). 6. Remove from 70%ETOH and place in 80% ETOH for 30min at RT. 7. Remove from 80% ETOH and place in 95% ETOH for 30 min at RT. 8. Remove from 95% ETOH and place in 100% ETOH(#1) for 30 min at RT. 9. Remove from 100% ETOH(#1) and place in 100% ETOH(#2) for 30 min at RT. 10. Remove from 100% ETOH (#2) and place in 1:1 (100%ETOH , 100% Xylene) for 30 min at RT. 11. Remove from 1:1 and place in 100% Xylene (#1) for 30 min at RT. 12. Remove from 100% Xylene (#1) and place in 100% Xylene (#2) for 30 min at RT. 13. Remove from 100% Xylene (#2) and place in 1:1 (100% Xylene, 100% paraffin) for 30 min in oven at 59C 14. Remove from 1:1 and place in 100% paraffin (#1) for 30 min in oven at 59C. 15. Remove from 100% paraffin (1#) and placed in 100% paraffin (#2) for 30 min in oven at 59C. 16. Remove and embed. 17. section tissue and look at under microscope. Please note that the paraffin that I am using is paraffin type 6 from Richard Allan Scientific with a melting point of 55-57c. We also have Histoplast PE from Thermo Scientific but haven?t used it. Any suggestions on how to solve this problem as well as any protocol one might be willing to share would be greatly appreciated. Thanks for your help. Bret TAMHSC From turkekul <@t> gmail.com Thu Aug 11 12:27:29 2011 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Thu Aug 11 12:27:34 2011 Subject: [Histonet] Re: Histonet Digest, Vol 93, Issue 14 In-Reply-To: <4e440abf.0bb6640a.2ccd.ffff8f04SMTPIN_ADDED@mx.google.com> References: <4e440abf.0bb6640a.2ccd.ffff8f04SMTPIN_ADDED@mx.google.com> Message-ID: We usually fix 16-20hrs in NBF, then do 5M EDTA pH=8.0 for 5-10 days at RT until the bone has soften. Place enough EDTA and change EDTA once in a while (like every other day) to avoid saturation of EDTA with Ca. After decalcificaiton is completed I re-fix the bone is NBF for another 6-8 hours. Then I transfer to 70% Ethanol for several hours and process for paraffin embedding. We use longer program that yours: Note: Make sure you use enough fixative and EDTA (15-20 times more than the volume of the tissue itself) . Make sure the tissue floats freely in the solution. You may even gently rotate while fixing. Make sure you wash with copious amount of tap water between and after each NBF or EDTA treatment. You can use harsher decal solutions for shorter times. EDTA is better to preserve the target macromolecules If you do IHC, IF or ISH. But your paraffin processing protocol seems short for that big bone you have. *Reagent * *Station* *Time (Hr:Min)* *Temp ?C* *P / V* *Drain Secs* *Stir* Ethanol 95% 1 2:00 AMB AMB 120 On Ethanol 95% 2 0:30 AMB AMB 120 On Abs Ethanol 3 0:30 AMB AMB 120 On Abs Ethanol 4 0:30 AMB AMB 120 On Abs Ethanol 5 0:30 AMB AMB 120 On Histoclear or xylene 6 0:45 AMB AMB 120 On Histoclear or xylene 7 0:45 AMB AMB 120 On Histoclear or xylene 8 1:00 AMB AMB 120 On 9 10 Paraffin Wax Left 1:30 59 P / V 120 On Paraffin Wax Middle 1:30 59 P / V 120 On Paraffin Wax Right 1:30 59 P / V 120 On Good luck! Mesru Turkekul turkekul@mskcc.org From mdpraet <@t> gmail.com Thu Aug 11 12:28:00 2011 From: mdpraet <@t> gmail.com (mequita praet) Date: Thu Aug 11 12:28:03 2011 Subject: [Histonet] traveling jobs Message-ID: I am interested in learning more about taking a traveling job. I need information. Which company do you histonetters recommend? Do you feel like there are still plenty of the traveling jobs available or has the economy taken a toll on them? I am considering relocating to Citrus County, FL and don't want to give up the job I have if traveling is not a good option since I can't find a job in that location. Thanks for any information you can give me. Mequita Praet, HTL (ASCP)SLS From turkekul <@t> gmail.com Thu Aug 11 12:34:22 2011 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Thu Aug 11 12:34:26 2011 Subject: [Histonet] thick sections and thaw freeze OCT tissues Message-ID: I cut 40-50 micron paraffin sections with no difficulty. You may try to use softer paraffin (lower melting temp) or adjust the blade angel. It seems like you have chatter due to hardness of the block or not appropriate blade angel. If tissue is over fixed or overprosessed and hence harder that it should be the thicker you cut the more difficult it will be. Mesruh Turkekul turkekum@mskcc.org From KMB01 <@t> grh.org Thu Aug 11 12:34:27 2011 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Thu Aug 11 12:34:32 2011 Subject: [Histonet] GROSSING Message-ID: We are looking at getting a pathologist resident for the first time. What can anyone tell me about what we need to allow him to do gross? Do we need a certificate of any kind? Thanks and have a great day. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From rjbuesa <@t> yahoo.com Thu Aug 11 14:21:23 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 11 14:21:27 2011 Subject: [Histonet] GROSSING In-Reply-To: Message-ID: <1313090483.46013.YahooMailClassic@web65706.mail.ac4.yahoo.com> A pathology resident is an MD going through his/her training to be certified as a pathologist. Isn't that enough? Ren? J. --- On Thu, 8/11/11, Kathy M. Gorham wrote: From: Kathy M. Gorham Subject: [Histonet] GROSSING To: histonet@lists.utsouthwestern.edu Date: Thursday, August 11, 2011, 1:34 PM We are looking at getting a pathologist resident for the first time. What can anyone tell me about what we need to allow him to do gross?? Do we need a certificate of any kind? Thanks and have a great day. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission.? If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heidi.Couture <@t> leica-microsystems.com Thu Aug 11 16:00:54 2011 From: Heidi.Couture <@t> leica-microsystems.com (Heidi.Couture@leica-microsystems.com) Date: Thu Aug 11 16:01:24 2011 Subject: [Histonet] AUTO: Heidi Couture is out of the office. (returning 08/15/2011) Message-ID: I am out of the office until 08/15/2011. I will be out of the office Thursday 8/11 & Friday 8/12, returning Monday 8/15, please call 1800-225-3035 (leica customer service) with any urgent matters, or email lisa.raymond@leica-microsystems.com. Please have your account number and PO available. Thank you, have a great day! Note: This is an automated response to your message "Histonet Digest, Vol 93, Issue 14" sent on 8/11/2011 11:53:19 AM. You will receive a notification for each message you send to this person while the person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From plucas <@t> biopath.org Thu Aug 11 18:14:59 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Thu Aug 11 18:15:04 2011 Subject: [Histonet] IHC on Pig help Message-ID: <20110811231459.5721C2F42@centaur.cnchost.com> Hello, We received a call today from a company that does research on animal tissue, and our sales rep asked me to check into this for him to see if we can add this to our list of services. First of all, we are an anatomical pathology lab that works with human tissues and we service hospitals and surgery centers in our area. The call we received today involves studies with yorkshire pigs. I am completely out my confort zone here and would love some feedback from anyone who can help me. I am sorry if I come across as being "stupid", but I really don't have any experience with animals, only as pets. I hope my questions aren't too bad. : ) The company is wanting to see any damage to the structure of the dermis after treatment, and they would like us to do the following IHC stains: (1) CD 68 (which I've heard of and we do, but on human tissue) (2) LBH (our sales rep told me it is Lactate Behybrogenase?) (3) Trypen Blue Who carries these antibodies? We use the Leica Bond with their reagents for human tissues. I'm assuming I need a whole new detection kit and antibodies for pig? If anyone can lead me in the right direction, I'd appreciate it. I need to find out how much the antibodies are, do I need a new detection kit, if we can get them in-house, and if we can stain them on the Bond. Thanks in advance...I'd really appreciate it Paula Lucas, HT BP Medical Group FV, CA From Diane.Tokugawa <@t> kp.org Thu Aug 11 19:32:08 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Thu Aug 11 19:32:18 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 08/11/2011 and will not return until 08/15/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, Barbara at 8-421-5033, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, general histology lab 8-421- 5408 or Wanda at 8-421-5426. From turkekul <@t> gmail.com Fri Aug 12 05:57:44 2011 From: turkekul <@t> gmail.com (Mesruh Turkekul) Date: Fri Aug 12 05:57:51 2011 Subject: [Histonet] Cell blocks In-Reply-To: <4e42b939.044b960a.2f0d.ffffd973SMTPIN_ADDED@mx.google.com> References: <4e42b939.044b960a.2f0d.ffffd973SMTPIN_ADDED@mx.google.com> Message-ID: <07DB2783-1E5E-42D5-A630-258C32E93C63@gmail.com> How do you prepare your cell blocks? Mesruh Turkekul MSc, MLT, HTL(ASCP)QIHC Turkekul@mskcc.org Sent from my iPad fern From turkekul <@t> gmail.com Fri Aug 12 06:09:09 2011 From: turkekul <@t> gmail.com (Mesruh Turkekul) Date: Fri Aug 12 06:09:16 2011 Subject: [Histonet] Sterilizing cassettes In-Reply-To: <4e42b939.044b960a.2f0d.ffffd973SMTPIN_ADDED@mx.google.com> References: <4e42b939.044b960a.2f0d.ffffd973SMTPIN_ADDED@mx.google.com> Message-ID: <1BBFAF58-C879-46D5-AA7B-1713F1237DD6@gmail.com> You can use sterilization without heat for plastic cassettes like ethylene oxide or UV. Most of the hospitals have ethylene oxide sterilizers. Or leave the empty labeled cassettes in formalin overnight and they will be sterilized. Mesruh Turkekul MSc, MLT, HTL(ASCP)QIHC turkekul@mskcc.org Sent from my iPad From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Aug 12 07:21:24 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Aug 12 07:23:21 2011 Subject: [Histonet] GROSSING In-Reply-To: <1313090483.46013.YahooMailClassic@web65706.mail.ac4.yahoo.com> References: , <1313090483.46013.YahooMailClassic@web65706.mail.ac4.yahoo.com> Message-ID: I believe that they also need to be supervised at all times. They cannot be left unsupervised, like on weekends or evenings. Please check this out, I know that our gross room recently had to change things due to the change in the resident rules. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com] Sent: Thursday, August 11, 2011 3:21 PM To: histonet@lists.utsouthwestern.edu; Kathy M. Gorham Subject: Re: [Histonet] GROSSING A pathology resident is an MD going through his/her training to be certified as a pathologist. Isn't that enough? Ren? J. --- On Thu, 8/11/11, Kathy M. Gorham wrote: From: Kathy M. Gorham Subject: [Histonet] GROSSING To: histonet@lists.utsouthwestern.edu Date: Thursday, August 11, 2011, 1:34 PM We are looking at getting a pathologist resident for the first time. What can anyone tell me about what we need to allow him to do gross? Do we need a certificate of any kind? Thanks and have a great day. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Fri Aug 12 07:48:47 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Aug 12 07:48:50 2011 Subject: [Histonet] hospitality events at NSH Message-ID: <1313153327.55257.YahooMailNeo@web130113.mail.mud.yahoo.com> Anyone heard anything about hospitality events at the NSH? Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From LStadler <@t> cbiolabs.com Fri Aug 12 08:49:57 2011 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Fri Aug 12 08:50:06 2011 Subject: [Histonet] sterilized cassette In-Reply-To: <20110810170700.C13961A556@barracuda.cbiolabs.com> References: <20110810170700.C13961A556@barracuda.cbiolabs.com> Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC3293F2@cbiolabs05.CBiolabs.local> Have you tried the "cell safe" cassette insert from Ted Pella? http://www.tedpella.com/embed_html/27141.htm#anchor27165 Cellsafe(tm) Biopsy Cassettes (white) shown open and closed & inserted into a regular tissue cassette. Cellsafe(tm) Biopsy Cassettes The Cellsafe(tm) is designed to contain small tissue fragments and biopsies during tissue processing. The Cellsafe(tm) Biopsy Insert with a hinged cover fits into a regular cassette. Top and bottom consist of extra fine nylon mesh preventing small tissues from getting lost during specimen preparation, keeping them safe. The plastic frame and nylon mesh are suitable for use in common tissue processing reagents including Formaldehyde solutions, Bouin's Fluid, Alcohol solutions, Xylene, Chloroform, Citrus based clearing agents and Paraffin wax (melting point up to 65?C). The plastic frame is made from the same material widely used in the manufacture of plastic processing cassettes. If you are using any unusual reagents it is suggested that you carry out preliminary chemical compatibility tests before using the Cellsafe(tm) to contain tissue samples. The Cellsafe(tm) is designed for single use only. Interior dimesions of a regular cassette are: 30 x 26 x 4.92mm H. Exterior dimensions of the Cellsafe Biopsy Cassettes are: 27.5 x 25 x 4.57mm H. Item # 21765, bag of 100 $40.85 Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 10, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 93, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: TFE-3 (Bernice Frederick) 2. What's your favorite phospho-histone H3 antibody for IHC? (Connolly, Brett M) 3. Re: Manual embedding (Collette, Nicole M.) 4. Fern tissue loss during in situ hybridization (Cordle, Angela R) 5. Re: Fern tissue loss during in situ hybridization (Rene J Buesa) 6. Sterilized cassette (Brandi Farris) 7. Re:2 (lynn13361@aol.com) 8. RE: Sterilized cassette (Mike Pence) 9. Cell block H&E staining (Dessoye, Michael J) 10. Re: Cell block H&E staining (Rene J Buesa) 11. Histotech needed in San Diego. Can you help? (Pam Barker) 12. CYTOLOGIST JOB OPENING PHILLY (rmweber113@comcast.net) 13. GROSSING SPECIMENS (Sara Baldwin/mhhcc.org) 14. Cyrostat Oppinions (McMahon, Loralee A) ---------------------------------------------------------------------- Message: 1 Date: Tue, 9 Aug 2011 18:05:51 +0000 From: Bernice Frederick Subject: [Histonet] RE: TFE-3 To: "Settembre, Dana" , "'Houston, Ronald'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E12D90D@evcspmbx3.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Same here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, August 09, 2011 11:05 AM To: 'Houston, Ronald'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: TFE-3 We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958 Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, August 09, 2011 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] TFE-3 Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 9 Aug 2011 15:09:47 -0400 From: "Connolly, Brett M" Subject: [Histonet] What's your favorite phospho-histone H3 antibody for IHC? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" ....p(Ser10) or p(Ser28) ? And have you found pHH3 (Ser28) to be more M-phase specific? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 3 Date: Tue, 9 Aug 2011 12:10:24 -0700 From: "Collette, Nicole M." Subject: Re: [Histonet] Manual embedding To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, All, I just saw this question and the responses, thought I would add my own solution to the mix. I do manual embedding, I use a hyb oven for my infiltrating/embedding station (like for hybridizing Southern or Northern blots- who does that anymore?). I have taken out the rotating wheel for spinning bottles, and line the tray at the bottom with foil. Temp control works very well. I have several Wheaton staining boxes (the kind that come with glass inserts for staining 20 slides) that I use for my wax changes for infiltrating, and a couple of metal beakers I use for pouring into molds. I have a little real estate left inside the oven (it's probably around 18"x 18" square area) to heat my molds for pouring, and I have a metal heat block in there that you would use for Eppendorf tubes as my forceps warmer. It works pretty well, but does take a long time to heat up those boxes of wax, so I need to plan ahead by about 3 hours. When I pour the molds, I have the oven in front of a drawer under the bench, I line an extra cabinet shelf with foil and lay it over the open drawer to give me some bench space, pour my molds inside the oven and transfer it to the foil-lined shelf to cool so I can move it without the specimen shifting. Then I transfer to a photo tray and cool in the fridge for a couple of hours before releasing the molds. With the door of the hyb oven open I have to embed in shifts and let the molds heat up again, but it works OK. At least it's all contained in one unit. It's hard to do histology in a molecular lab ;) Hope this helps to give a do-it-your-selfer some ideas. Sincerely, Nicole Collette LLNL On 8/2/11 7:53 PM, "Scott Parker" wrote: > Dear Histonetters: > > I am interested in acquiring a pitcher and heating jacket for melting and > pouring paraffin during manual embedding. My work is relatively low volume > and in a university research lab setting so I am trying to avoid purchasing > an expensive embedding station. Can anyone recommend an honest supplier of > used histology equipment that might be able to provide me with this item? > > Thank you for your expertise! > > Scott L. Parker > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 9 Aug 2011 20:46:03 +0000 From: "Cordle, Angela R" Subject: [Histonet] Fern tissue loss during in situ hybridization To: "histonet@lists.utsouthwestern.edu" Message-ID: <32F6F3C172009D44A3B5E6702B54DBF602A772D2@itsnt443.iowa.uiowa.edu> Content-Type: text/plain; charset="Windows-1252" Dear Histonet members, I?m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I?m not an expert at this process, but I?ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt?s coated slies), and I?ve had success with Arabidpsis ISH. I?ll outline the relevant and troubleshooting procedures that I?ve gone through here: 1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides. 2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides. 3. For mounting, I float tissue on 37?C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures. 4. I?ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48?C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results. 5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature. 5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH. Okay. So, here are my specific questions: Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide? What the beep should I try next? In advance, thank you all so much for taking the time to help me out with this frustrating problem! --Angie ------------------------------ Message: 5 Date: Tue, 9 Aug 2011 13:59:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Fern tissue loss during in situ hybridization To: "histonet@lists.utsouthwestern.edu" , Angela RCordle Message-ID: <1312923558.59874.YahooMailClassic@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 I think you should try to find out about the cellulose contents of the fern you are working with, and compare it to that of Arabidopsis. If the fern (Ceratopteris) contains more cellulose, I think this is the cause of your problem. If that is the case I think you should try to use a paraffin wax of higher melting point (63-65??C) to assure an infiltration more compatible with the cellulose. Also make sure that the infiltration is optimal. Finally if you can use thinner sections (<7 ??m) that could help also. Ren?? J. --- On Tue, 8/9/11, Cordle, Angela R wrote: From: Cordle, Angela R Subject: [Histonet] Fern tissue loss during in situ hybridization To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, August 9, 2011, 4:46 PM Dear Histonet members, I???m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I???m not an expert at this process, but I???ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt???s coated slies), and I???ve had success with Arabidpsis ISH. I???ll outline the relevant and troubleshooting procedures that I???ve gone through here: 1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides. 2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides. 3. For mounting, I float tissue on 37??C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures. 4. I???ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48??C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results. 5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature. 5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH. Okay. So, here are my specific questions: Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide? What the beep should I try next? In advance, thank you all so much for taking the time to help me out with this frustrating problem!?? ?? --Angie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 9 Aug 2011 19:46:25 -0500 From: Brandi Farris Subject: [Histonet] Sterilized cassette To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" My OR department is wanting a small biopsy cassette that they can sterilize, be in the sterile field and place small biopsy specimens directly into a cassette before placing in formalin. We've had a problem with small biopsies dissolving/disappearing in telfa pads. We can't seem to find a cassette that the manufacture approves to be sterilized. I have a sample of a metal biopsy cassette, but the lid closure is questionable. I'm having trouble thinking "out of the box" on this problem. I've never had an OR request this before. Any tips would be greatly appreciated! Thank you, Brandi Capital Region Medical Center Jefferson City, MO ------------------------------ Message: 7 Date: Wed, 10 Aug 2011 03:58:46 -0400 (EDT) From: lynn13361@aol.com Subject: [Histonet] Re:2 To: gmeinke@pima.edu, goffman48@msn.com, histo@nsh.org, histonet@lists.utsouthwestern.edu, booknerd@cox.net, info@casablancagardens.com Message-ID: <8CE254A777897B7-15C0-2CD98@Webmail-d108.sysops.aol.com> Content-Type: text/plain; charset="us-ascii"; format=flowed Hello! Tell me your feelings after trying this stuff!. http://aldeiadovale.com.br/com.page.php?pyhID=86tj5 ------------------------------ Message: 8 Date: Wed, 10 Aug 2011 07:57:27 -0500 From: "Mike Pence" Subject: RE: [Histonet] Sterilized cassette To: "Brandi Farris" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974C58@is-e2k3.grhs.net> Content-Type: text/plain; charset="US-ASCII" My question is this. Who will open the cassette and dictate the specimen and what if the specimen does not make it thru processing? Who is responsible then for it missing? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Farris Sent: Tuesday, August 09, 2011 7:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterilized cassette My OR department is wanting a small biopsy cassette that they can sterilize, be in the sterile field and place small biopsy specimens directly into a cassette before placing in formalin. We've had a problem with small biopsies dissolving/disappearing in telfa pads. We can't seem to find a cassette that the manufacture approves to be sterilized. I have a sample of a metal biopsy cassette, but the lid closure is questionable. I'm having trouble thinking "out of the box" on this problem. I've never had an OR request this before. Any tips would be greatly appreciated! Thank you, Brandi Capital Region Medical Center Jefferson City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 10 Aug 2011 10:35:01 -0400 From: "Dessoye, Michael J" Subject: [Histonet] Cell block H&E staining To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello all, I'm interested to see what staining protocol folks are using for H&E staining of cell blocks. Lately we've been getting varying light and dark staining on cell blocks only. Currently they are run on our standard H&E protocol that we use for all tissues. Does anyone use a separate protocol for cell blocks? Thanks! Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ------------------------------ Message: 10 Date: Wed, 10 Aug 2011 07:38:17 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Cell block H&E staining To: histonet@lists.utsouthwestern.edu, Michael JDessoye Message-ID: <1312987097.19039.YahooMailClassic@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The? staining variability you are obtaining on cell blocks is caused by the medium you are preparing them in. We always used the same H&E protocol and I do not think you will solve the problems by changing the H&E protocol, but by modifying the way you prepare the cell block. Ren? J. --- On Wed, 8/10/11, Dessoye, Michael J wrote: From: Dessoye, Michael J Subject: [Histonet] Cell block H&E staining To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 10, 2011, 10:35 AM Hello all, I'm interested to see what staining protocol folks are using for H&E staining of cell blocks.? Lately we've been getting varying light and dark staining on cell blocks only.? Currently they are run on our standard H&E protocol that we use for all tissues.? Does anyone use a separate protocol for cell blocks? Thanks! Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 10 Aug 2011 11:02:18 -0400 From: "Pam Barker" Subject: [Histonet] Histotech needed in San Diego. Can you help? To: "'Histonet'" Message-ID: <10BE35346E43450B80E74E70B6469EBE@ownerf1abaad51> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! How are you? I have a new histology position and I need your help. I am currently working with a leading edge cancer diagnostic laboratory in the San Diego area that is in need of a histotechnologist with strong immunohistochemistry experience. This is a permanent full time position and the schedule is 9a-530p Tuesday-Saturday. The client offers a great environment, a great crew to work with, excellent salary, great benefits and a generous relocation package. ASCP HT or HTL and a B.S. degree in a science related major are required. QIHC is a plus. My question is do you know of anyone who might be interested in this position? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 500.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 12 Date: Wed, 10 Aug 2011 15:47:29 +0000 (UTC) From: rmweber113@comcast.net Subject: [Histonet] CYTOLOGIST JOB OPENING PHILLY To: histonet Message-ID: <1743836975.17768.1312991249090.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 We have a part time job evening??opening for a cytologist to read urine cytology.?? The job is located in the Northeast Philadelphia area in a urology practice.?? Eligible candidates?? must have at least 3 years experience.?? Candidates can fax resumes to 215 947-2015 attention laboratory or call 215 947-4480. Thank you, ------------------------------ Message: 13 Date: Wed, 10 Aug 2011 11:47:42 -0400 From: "Sara Baldwin/mhhcc.org" Subject: [Histonet] GROSSING SPECIMENS To: "'Histonet'" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Histonetters If my Histo tech has an associates degree with enough chemistry courses can they gross the small specimens?? Acording to the new CAP, JOINT COMMISSION nad CLIAA regs?? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ------------------------------ Message: 14 Date: Wed, 10 Aug 2011 11:56:33 -0400 From: "McMahon, Loralee A" Subject: [Histonet] Cyrostat Oppinions To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi We are in the process of getting a new cyrostat and would like some opinions. We are looking at the ThermoFisher HM550. Please feel free to contact me offline if you like. Thank you in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 93, Issue 13 **************************************** This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From jqb7 <@t> cdc.gov Fri Aug 12 08:57:19 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Aug 12 08:57:24 2011 Subject: [Histonet] hospitality events at NSH In-Reply-To: <1313153327.55257.YahooMailNeo@web130113.mail.mud.yahoo.com> References: <1313153327.55257.YahooMailNeo@web130113.mail.mud.yahoo.com> Message-ID: Look under Networking on the Symposium page -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, August 12, 2011 8:49 AM To: Histonet Subject: [Histonet] hospitality events at NSH Anyone heard anything about hospitality events at the NSH? Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim <@t> ncpath.com Fri Aug 12 09:00:27 2011 From: Kim <@t> ncpath.com (Kimmie Rabe) Date: Fri Aug 12 09:00:27 2011 Subject: [Histonet] Online program for histotechnician program Message-ID: I am exploring the possibilty of recruiting a student to work at our facility and obtain histology training online (leading to a histotechnician certificate). I have found programs through the University of North Dakota, Indiana University School of Medicine, and Harford Community College in Maryland. Does anyone have any experience/insight to offer? Kimmie E. Rabe, MD North Central Pathology, PA 3701 12th Street North, Suite 201 St. Cloud, MN 56303 Phone: 320-253-6554 Fax: 320-253-1218 kim@ncpath.com From JWeems <@t> sjha.org Fri Aug 12 09:06:14 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Aug 12 09:06:26 2011 Subject: [Histonet] RE: Online program for histotechnician program In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408277BDB36@CHEXCMS10.one.ads.che.org> We have a program in south GA at Darton College. Our lab assistant just completed the program in June and passed her registry last week. I think the programs can be very successful. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe Sent: Friday, August 12, 2011 10:00 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Online program for histotechnician program I am exploring the possibilty of recruiting a student to work at our facility and obtain histology training online (leading to a histotechnician certificate). I have found programs through the University of North Dakota, Indiana University School of Medicine, and Harford Community College in Maryland. Does anyone have any experience/insight to offer? Kimmie E. Rabe, MD North Central Pathology, PA 3701 12th Street North, Suite 201 St. Cloud, MN 56303 Phone: 320-253-6554 Fax: 320-253-1218 kim@ncpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rjbuesa <@t> yahoo.com Fri Aug 12 09:34:43 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 12 09:34:46 2011 Subject: [Histonet] Online program for histotechnician program In-Reply-To: Message-ID: <1313159683.13982.YahooMailClassic@web65714.mail.ac4.yahoo.com> As far as I know, the program at the Indiana University School of Medicine is very good. Ren? J. --- On Fri, 8/12/11, Kimmie Rabe wrote: From: Kimmie Rabe Subject: [Histonet] Online program for histotechnician program To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, August 12, 2011, 10:00 AM I am exploring the possibilty of recruiting a student to work at our facility and obtain histology training online (leading to a histotechnician certificate).? I have found programs through the University of North Dakota, Indiana University School of Medicine, and Harford Community College in Maryland. Does anyone have any experience/insight to offer? Kimmie E. Rabe, MD North Central Pathology, PA 3701 12th Street North, Suite 201 St. Cloud, MN 56303 Phone:? 320-253-6554 Fax:? 320-253-1218 kim@ncpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Fri Aug 12 10:11:05 2011 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Fri Aug 12 10:11:35 2011 Subject: [Histonet] Sterilized cassette Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760879B290@VHAV09MSGA3.v09.med.va.gov> Why do they feel the need to put them in telfa pads? We receive very tiny biopsies floating in specimen container and the resident then uses a pipette (blood bank one with the tip cut off) them onto OBEX filter paper when they are grossed in. We also use a pipette to drop Mucicarmine on very small biopsies and core biopsies so that they are easily seen throughout grossing, embedding and cutting. The main reason to attach a biopsy or specimen to anything would be for orientation and if it is that small you wouldn't be able to ink it and place it in multiple cassettes let alone orient a cut surface for embedding. Maybe the surgeon could spend a few minutes in the lab to see how thing look after processing to embedding - maybe it would make sense to them then? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax Message: 8 Date: Wed, 10 Aug 2011 07:57:27 -0500 From: "Mike Pence" Subject: RE: [Histonet] Sterilized cassette To: "Brandi Farris" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974C58@is-e2k3.grhs.net> Content-Type: text/plain; charset="US-ASCII" My question is this. Who will open the cassette and dictate the specimen and what if the specimen does not make it thru processing? Who is responsible then for it missing? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Farris Sent: Tuesday, August 09, 2011 7:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterilized cassette My OR department is wanting a small biopsy cassette that they can sterilize, be in the sterile field and place small biopsy specimens directly into a cassette before placing in formalin. We've had a problem with small biopsies dissolving/disappearing in telfa pads. We can't seem to find a cassette that the manufacture approves to be sterilized. I have a sample of a metal biopsy cassette, but the lid closure is questionable. I'm having trouble thinking "out of the box" on this problem. I've never had an OR request this before. Any tips would be greatly appreciated! Thank you, Brandi Capital Region Medical Center Jefferson City, MO From brett_connolly <@t> merck.com Fri Aug 12 10:36:38 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Aug 12 10:36:44 2011 Subject: [Histonet] umbilical cord block Message-ID: Hi all, Would anyone be willing to donate an FFPE block of human umbilical for a student I am mentoring who enrolled in an on-line histology training program? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From jengirl1014 <@t> yahoo.com Fri Aug 12 10:52:11 2011 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Aug 12 10:52:14 2011 Subject: [Histonet] H&E for Frozen Tissue Message-ID: <1313164331.88113.YahooMailNeo@web125406.mail.ne1.yahoo.com> Does anyone have a good protocol for staining frozen sections with H&E?? Also, any mounting medium suggestions?? This is the first time I've been asked to do this for frozen. ? Thanks a bunch everyone! Jen ? Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 933 720 Rutland Avenue Baltimore, MD? 21205 phone:???? 410-614-0131 fax:???????? 410-955-9677 cell:???????? 443-631-6361 e-mail:? jsipes1@jhmi.edu From Marie.Ompoc <@t> cshs.org Fri Aug 12 11:00:47 2011 From: Marie.Ompoc <@t> cshs.org (Ompoc, Marie) Date: Fri Aug 12 11:02:02 2011 Subject: [Histonet] RE: umbilical cord block In-Reply-To: Message-ID: <2D17E59D8A1A80498172B62DB5CD3F75770141@cshsmsgmbx02.CSMC.EDU> Hi all, I'm interested to know about this online histology program? Can anybody give me the site? Thank you all! Thank You, Christine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, August 12, 2011 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] umbilical cord block Hi all, Would anyone be willing to donate an FFPE block of human umbilical for a student I am mentoring who enrolled in an on-line histology training program? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From brett_connolly <@t> merck.com Fri Aug 12 11:18:59 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Aug 12 11:19:11 2011 Subject: [Histonet] RE: umbilical cord block In-Reply-To: <2D17E59D8A1A80498172B62DB5CD3F75770141@cshsmsgmbx02.CSMC.EDU> References: <2D17E59D8A1A80498172B62DB5CD3F75770141@cshsmsgmbx02.CSMC.EDU> Message-ID: Hi Christine, Here is the link for the Harford Community College on-line Histotechnology Certification Program. http://www.harford.edu/cet/histotech/default.asp?FA=ContEd Best regards, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: Ompoc, Marie [mailto:Marie.Ompoc@cshs.org] Sent: Friday, August 12, 2011 12:01 PM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: umbilical cord block Hi all, I'm interested to know about this online histology program? Can anybody give me the site? Thank you all! Thank You, Christine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, August 12, 2011 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] umbilical cord block Hi all, Would anyone be willing to donate an FFPE block of human umbilical for a student I am mentoring who enrolled in an on-line histology training program? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From POWELL_SA <@t> mercer.edu Fri Aug 12 11:20:20 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Aug 12 11:20:27 2011 Subject: [Histonet] Online Histology program In-Reply-To: <2D17E59D8A1A80498172B62DB5CD3F75770141@cshsmsgmbx02.CSMC.EDU> References: <2D17E59D8A1A80498172B62DB5CD3F75770141@cshsmsgmbx02.CSMC.EDU> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DCD2A747@MERCERMAIL.MercerU.local> Darton College, Albany GA has an online program, go to http://www.darton.edu/programs/AlliedHealth/hist/ then click on the HT navigation to the right. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ompoc, Marie Sent: Friday, August 12, 2011 12:01 PM To: 'Connolly, Brett M'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: umbilical cord block Hi all, I'm interested to know about this online histology program? Can anybody give me the site? Thank you all! Thank You, Christine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, August 12, 2011 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] umbilical cord block Hi all, Would anyone be willing to donate an FFPE block of human umbilical for a student I am mentoring who enrolled in an on-line histology training program? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Fri Aug 12 11:47:43 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Fri Aug 12 11:47:47 2011 Subject: [Histonet] VALIDATION SHEET Message-ID: Hi Histonetters Does anyone have a sample validation sheet for immuno's like ER/PR etc?? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From gu.lang <@t> gmx.at Fri Aug 12 12:06:00 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Aug 12 12:06:07 2011 Subject: AW: [Histonet] GROSSING In-Reply-To: References: Message-ID: <6AC64C5BBB464A059E7CBCEF4AEDC08B@dielangs.at> Our residents are supervised by a senior pathologist as long as he/she thinks, that the resident is "mature" for selfresponsible grossing. They have the serious instruction to ask for help with any occuring problem/question. Our residents have an education time of four years. During this time a senior pathologist is responsible for their training to become a "ready-made" pathologist. In my experience the medical education is not enough for grossing without any further instruction. That depends of course on the type of specimens in your lab. Gudrun Histolab, Austria -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kathy M. Gorham Gesendet: Donnerstag, 11. August 2011 19:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] GROSSING We are looking at getting a pathologist resident for the first time. What can anyone tell me about what we need to allow him to do gross? Do we need a certificate of any kind? Thanks and have a great day. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Fri Aug 12 12:53:44 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Aug 12 12:53:46 2011 Subject: [Histonet] Online program for histotechnician program In-Reply-To: References: Message-ID: We've had several students go throught the Indiana University program, I believe that the program creats very good histotechs. On Fri, Aug 12, 2011 at 7:00 AM, Kimmie Rabe wrote: > I am exploring the possibilty of recruiting a student to work at our > facility and obtain histology training online (leading to a histotechnician > certificate). I have found programs through the University of North Dakota, > Indiana University School of Medicine, and Harford Community College in > Maryland. > > Does anyone have any experience/insight to offer? > > Kimmie E. Rabe, MD > North Central Pathology, PA > 3701 12th Street North, Suite 201 > St. Cloud, MN 56303 > Phone: 320-253-6554 > Fax: 320-253-1218 > kim@ncpath.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From Bryan.Watson <@t> neiurology.com Fri Aug 12 13:00:44 2011 From: Bryan.Watson <@t> neiurology.com (Bryan Watson) Date: Fri Aug 12 13:03:10 2011 Subject: [Histonet] Online program for histotechnician program In-Reply-To: References: , Message-ID: <1C03BD76DC9BD14EA266F05C65D173B4430F32FC20@NEIU-SBS.Niu.lan> I went through the IU program. I'd like to think I'm a swell histotech as a result. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreightl@gmail.com] Sent: Friday, August 12, 2011 1:53 PM To: Kimmie Rabe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Online program for histotechnician program We've had several students go throught the Indiana University program, I believe that the program creats very good histotechs. On Fri, Aug 12, 2011 at 7:00 AM, Kimmie Rabe wrote: > I am exploring the possibilty of recruiting a student to work at our > facility and obtain histology training online (leading to a histotechnician > certificate). I have found programs through the University of North Dakota, > Indiana University School of Medicine, and Harford Community College in > Maryland. > > Does anyone have any experience/insight to offer? > > Kimmie E. Rabe, MD > North Central Pathology, PA > 3701 12th Street North, Suite 201 > St. Cloud, MN 56303 > Phone: 320-253-6554 > Fax: 320-253-1218 > kim@ncpath.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Fri Aug 12 13:18:21 2011 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Fri Aug 12 13:18:29 2011 Subject: [Histonet] FW: Online Histology program Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Friday, August 12, 2011 12:20 PM To: Ompoc, Marie; 'Connolly, Brett M'; histonet@lists.utsouthwestern.edu Subject: [Histonet] Online Histology program Darton College, Albany GA has an online program, go to http://www.darton.edu/programs/AlliedHealth/hist/ then click on the HT navigation to the right. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ompoc, Marie Sent: Friday, August 12, 2011 12:01 PM To: 'Connolly, Brett M'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: umbilical cord block Hi all, I'm interested to know about this online histology program? Can anybody give me the site? Thank you all! Thank You, Christine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, August 12, 2011 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] umbilical cord block Hi all, Would anyone be willing to donate an FFPE block of human umbilical for a student I am mentoring who enrolled in an on-line histology training program? Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From Annette_hall <@t> pa-ucl.com Fri Aug 12 13:58:52 2011 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Fri Aug 12 13:58:56 2011 Subject: [Histonet] Online program for histotechnician program In-Reply-To: References: Message-ID: <8B8724C83737DF41888FFD6ECCB161B147127C@PEITHA.wad.pa-ucl.com> We have sent 6 staff members to the Univ of Indiana over the past 15 yrs. Excellent program=excellent techs. Annette J Hall, MT Micro/Histo/Cyto Supervisor United Clinical Laboratories 205 Bluff St Ste 1 Dubuque, IA 52001 Ph: 563.556.2010 x131 Fax: 563.584.2085 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Friday, August 12, 2011 12:54 PM To: Kimmie Rabe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Online program for histotechnician program We've had several students go throught the Indiana University program, I believe that the program creats very good histotechs. On Fri, Aug 12, 2011 at 7:00 AM, Kimmie Rabe wrote: > I am exploring the possibilty of recruiting a student to work at our > facility and obtain histology training online (leading to a histotechnician > certificate). I have found programs through the University of North Dakota, > Indiana University School of Medicine, and Harford Community College in > Maryland. > > Does anyone have any experience/insight to offer? > > Kimmie E. Rabe, MD > North Central Pathology, PA > 3701 12th Street North, Suite 201 > St. Cloud, MN 56303 > Phone: 320-253-6554 > Fax: 320-253-1218 > kim@ncpath.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From SJMccabe <@t> drmc.org Fri Aug 12 14:07:23 2011 From: SJMccabe <@t> drmc.org (McCabe, Sara J.) Date: Fri Aug 12 14:11:06 2011 Subject: [Histonet] RE: controls on patient slide Message-ID: <02AE2390303AAB43A823930EAD6B63AE1C46B9CC@EX07.drmc.org> We always put our patient and control tissue on the same slide. We have many controls already cut and then we place the patient tissue below. If we are using blanks that were previously cut then we cut the control and place that below the patient tissue. We chose to not invest in the pre-marked slides. After processing we make a box with a red sharpie around the control tissue so there is no confusion as to which is the patient tissue and which is control. Sara McCabe, HT (ASCP) DuBois Regional Medical Center ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, July 21, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 92, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Assisting with Autopsies (Horn,Hazel) 2. Re: Leica Service Technician (Nicole Tatum) 3. RE: Leica Service Technician (Suresch, Donna L.) 4. Research microtomes (Adrienne Anderson) 5. Re: Research microtomes (Jack Ratliff) 6. Re: Research microtomes (Adrienne Anderson) 7. Re: Research microtomes (Jack Ratliff) 8. embalmers using formaldehyde - NYT story (Bob Richmond) 9. Re: Microtome draft shield (Joseph Saby) 10. Re: (Denise Piontek) 11. Re: Research microtomes (Adrienne Anderson) 12. Re: Research microtomes (Jack Ratliff) 13. Small Slide Scanner (Sheila Haas) 14. Fast Blue stabilization (carpj) ---------------------------------------------------------------------- Message: 1 Date: Wed, 20 Jul 2011 12:17:48 -0500 From: "Horn,Hazel" Subject: [Histonet] RE: Assisting with Autopsies To: "'Amy Self'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <25A4DE08332B19499904459F00AAACB7198B85ACEA@EVS1.archildrens.org> Content-Type: text/plain; charset="us-ascii" We have an autopsy assistant and when he's not busy with autopsies (which is often) he works in our histology lab as an assistant!! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 2:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 2 Date: Wed, 20 Jul 2011 14:08:10 -0400 (EDT) From: "Nicole Tatum" Subject: Re: [Histonet] Leica Service Technician To: "Amanda Madden" , histonet@lists.utsouthwestern.edu Message-ID: <3934.208.62.167.196.1311185290.squirrel@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 Amanda, Im not exactly sure which part of your machine is freezing based on your description, but, I have a leica 1510 and it has freezing issues. Everyone I have ever used does this. The bar that hold the specimen chucks ices and freezes over like crazy.. It will frezze the chuck in the bar holder solid. I have to get a hammer and beat it sometimes..lol. So, before I use my machine I wipe 100% alcohol across the bar to de-ice it and the chucks dont get stuck..Do not use so much that you lower your temp. Also, do not get on the oct or the stage because your blocks will not cut and be mush... Just wipe the areas daily with alcohol.. Hope this helps. Nicole Tatum, HT ASCP Hi Histonetters! > > A few months back I emailed regarding a service contract through Leica for > our CM 3050S, and (unfortunately?) we chose not to purchase one. This week > we have had a serious issue with it... the specimen head and arm is > covered > in frost, and the object temperature sensor is reading ## instead of a > temp. > In any case, we called Leica and asked for a service call, but it is > extremely expensive and they couldn't give us an estimate of when they > will > be here because cryostats used for clinical applications have priority > over > those, like ours, that are used for research. Understandable, but > frustrating nonetheless. So my question is: does anyone know of a good, > reputable cryostat service technician (who is authorized by leica, if > possible) that is located in the Boston, MA area? > > Any help would be greatly appreciated! > > Thanks in advance, > Amanda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Wed, 20 Jul 2011 14:16:40 -0400 From: "Suresch, Donna L." Subject: [Histonet] RE: Leica Service Technician To: "histonet@lists.utsouthwestern.edu" Message-ID: <2C9A1D9608959940943F357E0A470FF89765A7FB5A@USCTMXP51005.merck.com> Content-Type: text/plain; charset="us-ascii" Hello Amanda, We are located in Eastern Pa and we use Belair Instruments out of NJ (908-518-2662). We have been very happy with their service on our cryostats. Donna L. Suresch Merck Research Laboratories Research Biologist Imaging Research - West Point -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 20, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 92, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Leica Service Technician (Amanda Madden) 2. Controls with patient specimen on same slide (SHargrove@urhcs.org) 3. please remove me from the list, thank you! (Robert Cordero) 4. RE: Leica Service Technician (Sherwood, Margaret ) 5. Cat Scratch control (Houston, Ronald) 6. RE: Leica Service Technician (Tom McNemar) 7. RE: Controls with patient specimen on same slide (Tom McNemar) 8. RE: Assisting with Autopsies (Weems, Joyce) 9. Controls with patient specimen on same slide (Nancy Schmitt) ---------------------------------------------------------------------- Message: 1 Date: Wed, 20 Jul 2011 11:32:07 -0400 From: Amanda Madden Subject: [Histonet] Leica Service Technician To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda ------------------------------ Message: 2 Date: Wed, 20 Jul 2011 10:35:33 -0500 From: SHargrove@urhcs.org Subject: [Histonet] Controls with patient specimen on same slide To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" In our Lab we put a control on every slide. We keep multiple controls cut and stored in refrigerator. Most are multi- tissue controls. We use the Ventana detection kits and it is not cost efficient to use 2 uses of the detection kit per antibody. Of course there are times that this will not work, then our cost per test goes up, but we cannot charge any more for a control. (Embedded image moved to file: pic32000.jpg) ------------------------------ Message: 3 Date: Wed, 20 Jul 2011 15:37:44 +0000 From: Robert Cordero Subject: [Histonet] please remove me from the list, thank you! To: "Histonet@lists.utsouthwestern.edu" Message-ID: <0BA36F96367E8A4CBB27E112FAA6488A0679E9@vslcexmbp02.mychg.com> Content-Type: text/plain; charset="us-ascii" ------------------------------ Message: 4 Date: Wed, 20 Jul 2011 11:43:46 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] Leica Service Technician To: "Amanda Madden" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB58D3@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" Amanda, We have a Microm HM550 cryostat and have used Brian J. Hurley (New England Biomedical Services). He is an independent service engineer. He's very good. In fact ThermoFisher uses him if a service visit is needed. 781-331-8642 617-774-7368 (cell) Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 5 Date: Wed, 20 Jul 2011 15:55:50 +0000 From: "Houston, Ronald" Subject: [Histonet] Cat Scratch control To: "'histonet@lists.utsouthwestern.edu'" , "ihcrg@googlegroups.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have an extra Cat Scratch control block to spare? We currently buy control sections from Newcomer, but I'm not convinced they are that good. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 6 Date: Wed, 20 Jul 2011 12:26:11 -0400 From: Tom McNemar Subject: RE: [Histonet] Leica Service Technician To: 'Amanda Madden' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" It is really just a freezer with a microtome in it. Do you have an on-site refrigeration guy that could look at it? I have used our in-house guy a time or two. Ours is pretty simple and does not have all of the electronics that you may have on the Leica but it is worth a shot. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 7 Date: Wed, 20 Jul 2011 12:30:06 -0400 From: Tom McNemar Subject: RE: [Histonet] Controls with patient specimen on same slide To: "'Rathborne, Toni'" , 'Richard Cartun' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Each case gets one positive control per antibody. If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" 7/19/2011 >>> 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 8 Date: Wed, 20 Jul 2011 12:53:21 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Assisting with Autopsies To: Amy Self , "'histonet@lists.utsouthwestern.edu'" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082122A73D@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We have two contract deiners.. They work at other hospitals around town and charge per case. Works well here as our numbers have decreased drastically over the years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 15:12 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 9 Date: Wed, 20 Jul 2011 16:59:53 +0000 From: Nancy Schmitt Subject: [Histonet] Controls with patient specimen on same slide To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36791511@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" We place control tissue on EACH slide for IHC testing - even if multiple blocks from same case. Nancy Schmitt HT, MLT(ASCP) United Clinical Laboratories ----------------------------------------- Message: 12 Date: Wed, 20 Jul 2011 13:18:52 +0000 From: "Rathborne, Toni" Subject: RE: [Histonet] Controls with patient specimen on same slide To: "'Richard Cartun'" , "histonet@lists.utsouthwestern.edu" Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707031366@SMCMAIL01.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 27 **************************************** Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 4 Date: Wed, 20 Jul 2011 12:48:14 -0700 (PDT) From: Adrienne Anderson Subject: [Histonet] Research microtomes To: "histonet@lists.utsouthwestern.edu" Cc: "bushma1@rose-hulman.edu" , "mlosborn2@gmail.com" Message-ID: <1311191294.91032.YahooMailNeo@web59614.mail.ac4.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ------------------------------ Message: 5 Date: Wed, 20 Jul 2011 16:02:19 -0500 From: Jack Ratliff Subject: Re: [Histonet] Research microtomes To: Adrienne Anderson Cc: "histonet@lists.utsouthwestern.edu" , "bushma1@rose-hulman.edu" , "mlosborn2@gmail.com" Message-ID: Content-Type: text/plain; charset="us-ascii" What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: > Hello Histo-land, > > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Wed, 20 Jul 2011 14:57:48 -0700 (PDT) From: Adrienne Anderson Subject: Re: [Histonet] Research microtomes To: Jack Ratliff Cc: "histonet@lists.utsouthwestern.edu" , "bushma1@rose-hulman.edu" , "mlosborn2@gmail.com" Message-ID: <1311199068.60287.YahooMailNeo@web59611.mail.ac4.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Jack, We're trying to cut just plain old FFPE blocks. ________________________________ From: Jack Ratliff To: Adrienne Anderson Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: > Hello Histo-land, > > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Wed, 20 Jul 2011 17:13:43 -0500 From: Jack Ratliff Subject: Re: [Histonet] Research microtomes To: Adrienne Anderson Cc: "histonet@lists.utsouthwestern.edu" , "bushma1@rose-hulman.edu" , "mlosborn2@gmail.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson wrote: > Hi Jack, > > We're trying to cut just plain old FFPE blocks. > > From: Jack Ratliff > To: Adrienne Anderson > Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" > Sent: Wednesday, July 20, 2011 5:02 PM > Subject: Re: [Histonet] Research microtomes > > What type of specimen are you trying to cut? What embedding media are you using? > > Jack > > > > On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: > > > Hello Histo-land, > > > > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! > > > > Thanks, > > Adrienne > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ Message: 8 Date: Wed, 20 Jul 2011 18:45:19 -0400 From: Bob Richmond Subject: [Histonet] embalmers using formaldehyde - NYT story To: patho-l@mailman.srv.ualberta.ca, "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 This New York Times story about the use of formaldehyde by embalmers is worth reading by pathologists and histotechnologists. http://www.nytimes.com/2011/07/21/business/despite-cancer-risk-embalmers-stay-with-formaldehyde.html?_r=1&hp Seems like they've basically got the right idea. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 9 Date: Wed, 20 Jul 2011 15:45:47 -0700 (PDT) From: Joseph Saby Subject: Re: [Histonet] Microtome draft shield To: "Rathborne, Toni" , 'Keri Colwell' , "histonet@lists.utsouthwestern.edu" Message-ID: <1311201947.45523.YahooMailNeo@web114401.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I would suggest that you look inthe local yellow pages for people who work with plexiglass.? This is a re;atively inexpensive medium.? You can design what you need and have your draft shields built to order. ? Joe Saby NAMSA From: "Rathborne, Toni" To: 'Keri Colwell' ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, July 19, 2011 9:11 AM Subject: RE: [Histonet] Microtome draft shield Haven't seen anything that specific, but you might want to look at the various biohazard splash guards. They are a clear Plexiglas, and they have a base to support them. You would be able to move them around (or have them mounted to the counter if desired), and they come in an assortment of angles and sizes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Monday, July 18, 2011 5:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome draft shield Hi Everyone, I work in a lab which is under negative pressure (air is continuously flowing in), and due to the layout of the rooms are microtomes are located next to two different doorways.? We are looking for some sort of draft shield to place around each microtome and water bath that will reduce the effects of the airflow and personnel movements on our ribbons. Anyone have any suggestions as to who might sell such a thing? Thanks in advance! Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640? | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell@inspection.gc.ca Telephone | T?l?phone:? 403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 21 Jul 2011 09:11:33 -0400 From: Denise Piontek Subject: [Histonet] Re: To: , , , , , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Sexual problems are to be solved... http://ravera.todouy.com/bestsite.php?opage=20f0 ------------------------------ Message: 11 Date: Thu, 21 Jul 2011 07:49:22 -0700 (PDT) From: Adrienne Anderson Subject: Re: [Histonet] Research microtomes To: Jack Ratliff Cc: "histonet@lists.utsouthwestern.edu" , "bushma1@rose-hulman.edu" , "mlosborn2@gmail.com" Message-ID: <1311259762.22661.YahooMailNeo@web59603.mail.ac4.yahoo.com> Content-Type: text/plain; charset=us-ascii This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: "In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns." Thanks, Adrienne ________________________________ From: Jack Ratliff To: Adrienne Anderson Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" Sent: Wednesday, July 20, 2011 6:13 PM Subject: Re: [Histonet] Research microtomes Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson wrote: Hi Jack, > >We're trying to cut just plain old FFPE blocks. > > > >________________________________ >From: Jack Ratliff >To: Adrienne Anderson >Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" >Sent: Wednesday, July 20, 2011 5:02 PM >Subject: Re: [Histonet] Research microtomes > >What type of specimen are you trying to cut? What embedding media are you using? > >Jack > > > >On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: > >> Hello Histo-land, >> >> I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! >> >> Thanks, >> Adrienne >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ Message: 12 Date: Thu, 21 Jul 2011 10:07:09 -0500 From: Jack Ratliff Subject: Re: [Histonet] Research microtomes To: Adrienne Anderson Cc: "histonet@lists.utsouthwestern.edu" , "bushma1@rose-hulman.edu" , "mlosborn2@gmail.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Thanks a lot for your response Adrienne! I think I may be able to help you with this. Is there a time we can talk off-line over the phone? My number is 317-281-1975. Best Regards, Jack On Jul 21, 2011, at 9:49 AM, Adrienne Anderson wrote: > This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: > > "In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. > > Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns." > > Thanks, > Adrienne > > From: Jack Ratliff > To: Adrienne Anderson > Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" > Sent: Wednesday, July 20, 2011 6:13 PM > Subject: Re: [Histonet] Research microtomes > > Couple more questions. :) What is the tissue and the dimensions of the specimen? > > > > On Jul 20, 2011, at 4:57 PM, Adrienne Anderson wrote: > >> Hi Jack, >> >> We're trying to cut just plain old FFPE blocks. >> >> From: Jack Ratliff >> To: Adrienne Anderson >> Cc: "histonet@lists.utsouthwestern.edu" ; "bushma1@rose-hulman.edu" ; "mlosborn2@gmail.com" >> Sent: Wednesday, July 20, 2011 5:02 PM >> Subject: Re: [Histonet] Research microtomes >> >> What type of specimen are you trying to cut? What embedding media are you using? >> >> Jack >> >> >> >> On Jul 20, 2011, at 2:48 PM, Adrienne Anderson wrote: >> >> > Hello Histo-land, >> > >> > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! >> > >> > Thanks, >> > Adrienne >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >> > > ------------------------------ Message: 13 Date: Thu, 21 Jul 2011 08:34:10 -0700 (PDT) From: Sheila Haas Subject: [Histonet] Small Slide Scanner To: histonet@lists.utsouthwestern.edu Message-ID: <1311262450.88288.YahooMailRC@web161715.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all. Is anyone out there using one of the small, low volume slide scanners for use in?frozen sections? We have?looked at a couple of the larger scanners for other purposes but?wanted to know how the smaller ones would?work for our pathologists to?review frozen slides from off-site facilities. Of course, a PA would gross?the specimen at the facility,?cut the slides and?then load them into the scanner. Any thoughts? ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ------------------------------ Message: 14 Date: Thu, 21 Jul 2011 12:36:59 -0400 From: carpj Subject: [Histonet] Fast Blue stabilization To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hi List, We are retrogradely labeling spinal motoneurons with Fast Blue, which works well. When we process our sections (60-?m) for immunohistochemical labeling, the Fast Blue signal becomes much weaker. We believe that the detergents we are using to facilitate antibody penetration are also allowing the Fast Blue to leak out of the cells. Is anyone aware of methods for stabilizing Fast Blue within the cells so it won't wash out during immunohistochemical processing? Jonathan Carp, Ph.D. Wadsworth Center New York State Dept. Health Albany, NY IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 28 **************************************** From SJMccabe <@t> drmc.org Fri Aug 12 14:32:22 2011 From: SJMccabe <@t> drmc.org (McCabe, Sara J.) Date: Fri Aug 12 14:35:17 2011 Subject: [Histonet] RE: p16 on the Bond Message-ID: <02AE2390303AAB43A823930EAD6B63AE1C46B9CD@EX07.drmc.org> Hi Nancy, We too use p16 on the Bond Max and Bond III using Leica ER 1 for 20 min. It is working great for us! Sara McCabe, HT (ASCP) DuBois Regional Medical Center ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Monday, July 18, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 92, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Invitation to connect on LinkedIn (Sheree Holmes via LinkedIn) 2. Water droplets on slides (Esther Peters) 3. RNAlater buffer & fixation/embedding in OCT (Megan French) 4. positive staining on my secondary only controls...need a DAB superquench? (Megan French) 5. Lab humidity range (L White) 6. P16 on the BOND (Nancy Schmitt) 7. Re: Histobath revised (Johnson, Teri) 8. Alcian blue-safranin (Figliuolo, Leticia) 9. RE: Re: Histobath revised (Houston, Ronald) 10. RE: positive staining on my secondary only controls...need a DAB superquench? (Elizabeth Chlipala) 11. RE: P16 on the BOND (Settembre, Dana) 12. Spirabrush (Gauch, Vicki) 13. Leica SELECTECH (Breeden, Sara) 14. acceptance criteria of IHC stains (Elizabeth Chlipala) 15. Peggy Wenk's job postings (Lee & Peggy Wenk) ---------------------------------------------------------------------- Message: 1 Date: Sun, 17 Jul 2011 22:10:33 +0000 (UTC) From: Sheree Holmes via LinkedIn Subject: [Histonet] Invitation to connect on LinkedIn To: David Edmondson Message-ID: <1217399822.5174163.1310940633806.JavaMail.app@ela4-bed81.prod> Content-Type: text/plain; charset=UTF-8 LinkedIn ------------ Sheree Holmes requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Sheree Accept invitation from Sheree Holmes http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I133508382_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYOe3cUc3kPcP59bT9NtPlhgCBAbPgTc3cNdPcSdjcLrCBxbOYWrSlI/EML_comm_afe/ View invitation from Sheree Holmes http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I133508382_13/3cNnP8UcPwMdjcPckALqnpPbOYWrSlI/svi/ ------------------------------------------ DID YOU KNOW your LinkedIn profile helps you control your public image when people search for you? Setting your profile as public means your LinkedIn profile will come up when people enter your name in leading search engines. Take control of your image! http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/ewp/inv-22/ -- (c) 2011, LinkedIn Corporation ------------------------------ Message: 2 Date: Sun, 17 Jul 2011 19:10:36 -0400 From: Esther Peters Subject: [Histonet] Water droplets on slides To: Histonet@lists.utsouthwestern.edu Message-ID: <4E236BEC.90509@gmu.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I am having problems with water droplets appearing on slides when transferring the rack from the last 100% dehydrant (Richard-Allan) to either SafeClear II or xylenes. I saw that some of you had noted this problem might be in the xylene substitute, but switching to xylene seemed to solve it. I am wondering if the "100%" isn't? I have poured immediately before use from freshly opened bottles, and still have the problem. Would appreciate any help (of course, the humidity is high right now, but...)! -- Esther C. Peters, Ph.D., Assistant Professor; Department of Environmental Science & Policy; George Mason University ------------------------------ Message: 3 Date: Mon, 18 Jul 2011 13:43:25 +1000 From: "Megan French" Subject: [Histonet] RNAlater buffer & fixation/embedding in OCT To: Message-ID: Content-Type: text/plain; charset="us-ascii" Has anyone successful fixed & embedded tissues in OCT after using RNAlater buffer? I've found a paper that says this doesn't affect tissue integrity but I need for info regarding protocol (To defrost? Fixative? Timing? Temperature Etc) ...and more than anything feedback from someone who has used/uses this product. Product: http://www.ambion.com/techlib/resources/RNAlater/ Paper: http://www.nature.com/modpathol/journal/v14/n2/pdf/3880267a.pdf Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne) Research Assistant Surgical Research; Infection & Immunity Theme Murdoch Children's Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia E megan.french@mcri.edu.au * ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 4 Date: Mon, 18 Jul 2011 13:44:55 +1000 From: "Megan French" Subject: [Histonet] positive staining on my secondary only controls...need a DAB superquench? To: Message-ID: Content-Type: text/plain; charset="us-ascii" Having some trouble with my IHC optimisation. Hopefully it's a simple fix someone may be able to provide some guidance (Im a junior RA! Hello histoworld!). Im using a DAB protocol with swine-anti-rabbit HRP on human colon. I have successful staining of my primary (yay!), somewhat successful staining with my antibody preabsorbed with peptide (yet still some staining)...BUT im seeing positive staining on my secondary only controls (may explain why im getting a bit of staining on my preabsorped specimens). I quench with 5% hydrogen peroxide for 5 min on day 1 (prior to primaries/no primary if secondary control) and on day 2 add 1 DAKO DAB chromogen tablet to 10ml PBS and 10ul hydrogen peroxide again before immersing slides for 8 min, then stain with haematoxylin per usual, dehydrate, clear & mount. I feel like I need a super quencher or something?! Any tips would be most helpful. (My slides also undergo antigen retrieval in citrate buffer ph6 for 10min @ 95deg and 20min at RT) Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne) Research Assistant Surgical Research; Infection & Immunity Theme Murdoch Children's Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia E megan.french@mcri.edu.au * ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 5 Date: Mon, 18 Jul 2011 06:27:00 -0400 From: L White Subject: [Histonet] Lab humidity range To: Message-ID: Content-Type: text/plain; charset="us-ascii" Sakura has developed a threshold for humidity if you are using their film coverslipper. Other than that, I am not aware of any specific requirements for routine histology equipment. Lori ------------------------------ Message: 6 Date: Mon, 18 Jul 2011 13:24:28 +0000 From: Nancy Schmitt Subject: [Histonet] P16 on the BOND To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36791319@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Happy Monday- I should have been more specific in my question - is anyone using p16 on the BOND IHC instrument? Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 7 Date: Mon, 18 Jul 2011 08:44:24 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: Histobath revised To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF977056669@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Dr. Richmond recommended using non-flammable and non-explosive Novec (tm) Engineered Fluid HFE-7100. We are currently using this for our freezing and it works very well. The main thing we have noticed is the blocks float in it and do not sink as they would with other solvents. It is a 3M product, and my contact there last year was ebinder@mmm.com (Erin Binder). Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO ------------------------------ Message: 8 Date: Mon, 18 Jul 2011 09:44:39 -0400 From: "Figliuolo, Leticia" Subject: [Histonet] Alcian blue-safranin To: "histonet@lists.utsouthwestern.edu" Message-ID: <069E6CF048B915488720412DAFD1474D03FC377975@RNUMSEM702.nala.roche.com> Content-Type: text/plain; charset="us-ascii" Hello everybody, I was wondering if anyone has a protocol for Alcian Blue-Safranin (for mast cells). I would greatly appreciate any help. Thank you! Leticia Figliuolo e-mail: leticia.figliuolo@roche.com ------------------------------ Message: 9 Date: Mon, 18 Jul 2011 13:57:38 +0000 From: "Houston, Ronald" Subject: [Histonet] RE: Re: Histobath revised To: "'Johnson, Teri'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I second Terri's comments Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Monday, July 18, 2011 9:44 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Histobath revised Dr. Richmond recommended using non-flammable and non-explosive Novec (tm) Engineered Fluid HFE-7100. We are currently using this for our freezing and it works very well. The main thing we have noticed is the blocks float in it and do not sink as they would with other solvents. It is a 3M product, and my contact there last year was ebinder@mmm.com (Erin Binder). Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 10 Date: Mon, 18 Jul 2011 08:32:44 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] positive staining on my secondary only controls...need a DAB superquench? To: 'Megan French' , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA051A@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Megan Pre absorbing your antibody for a control for IHC is not always going to work there are publications out there that do not recommend it. I'll try to find the reference I'm talking about. I pulled this out of one of my presentations Protocols available on the web - for western blots http://www.upstate.com/misc/protocol_detail.q.prot.e.peptide-competion.a.name.e.Peptide_Competition http://store.crpinc.com/prot_peptide_comp.aspx "The absorption control is less important because if cannot determine whether the protein bound in the tissue is the same protein that used for absorption. Therefore, the recommended guidelines for immunohistochemistry include negative controls and positive controls but do not include absorption controls." Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Megan French Sent: Sunday, July 17, 2011 9:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] positive staining on my secondary only controls...need a DAB superquench? Having some trouble with my IHC optimisation. Hopefully it's a simple fix someone may be able to provide some guidance (Im a junior RA! Hello histoworld!). Im using a DAB protocol with swine-anti-rabbit HRP on human colon. I have successful staining of my primary (yay!), somewhat successful staining with my antibody preabsorbed with peptide (yet still some staining)...BUT im seeing positive staining on my secondary only controls (may explain why im getting a bit of staining on my preabsorped specimens). I quench with 5% hydrogen peroxide for 5 min on day 1 (prior to primaries/no primary if secondary control) and on day 2 add 1 DAKO DAB chromogen tablet to 10ml PBS and 10ul hydrogen peroxide again before immersing slides for 8 min, then stain with haematoxylin per usual, dehydrate, clear & mount. I feel like I need a super quencher or something?! Any tips would be most helpful. (My slides also undergo antigen retrieval in citrate buffer ph6 for 10min @ 95deg and 20min at RT) Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne) Research Assistant Surgical Research; Infection & Immunity Theme Murdoch Children's Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia E megan.french@mcri.edu.au * ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 18 Jul 2011 11:02:37 -0400 From: "Settembre, Dana" Subject: [Histonet] RE: P16 on the BOND To: 'Nancy Schmitt' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Nancy, I am using the p16 with the Bond. I am using the Bond's ER1 for 20min with their Refine detection kit. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Monday, July 18, 2011 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P16 on the BOND Happy Monday- I should have been more specific in my question - is anyone using p16 on the BOND IHC instrument? Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 18 Jul 2011 11:16:57 -0400 From: "Gauch, Vicki" Subject: [Histonet] Spirabrush To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I was wondering if anyone has had any experience with processing specimens obtained using the Spirabrush for GYN cases. We have an account who would like to submit his specimens using this method and we wanted to know if people are handling these as Cytology specimens or Histology specimens. Any information would be greatly appreciated. Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ------------------------------ Message: 13 Date: Mon, 18 Jul 2011 10:05:18 -0600 From: "Breeden, Sara" Subject: [Histonet] Leica SELECTECH To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF8C1@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Need to speak with a Technical contact regarding SelecTech staining system - someone who can give details about expiration dates. Cannot find contact info online. Thanks! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ Message: 14 Date: Mon, 18 Jul 2011 10:14:23 -0600 From: Elizabeth Chlipala Subject: [Histonet] acceptance criteria of IHC stains To: "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0524@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Hello everyone Has anyone out there had to write acceptance criteria for a USP document, any help would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 ------------------------------ Message: 15 Date: Mon, 18 Jul 2011 12:43:56 -0400 From: "Lee & Peggy Wenk" Subject: [Histonet] Peggy Wenk's job postings To: "Histonet" Message-ID: <033A63EE883E4A36AFBE246D19F2A0DB@HP2010> Content-Type: text/plain; charset="iso-8859-1" Histonetters - There seems to be a little glitch in our hospital's application process. Several people have said that they have applied for the position of Program Director for the Schools of Histotechnology at William Beaumont Hospital, Royal Oak, MI. But no one is showing up in the system. Could all those interested please go two things: 1. Reapply 2. Send resume (in a separate email, in addition to attaching it to the application process) to our Human Resources person and to me, just in case the application doesn't show up again. Diane Soper, Human Resources dsoper@beaumont.edu Peggy Wenk, School, pwenk@beaumont.edu For more information about the job, and directions on where to apply, go to the NSH website: www.nsh.org On the right, click on Career Center On the left/middle, click on View Jobs Look for the 6/30/11 Program Director position, click on View I apologize for the inconvenience. Peggy A. Wenk, HTL(ASCP)SLS Program Director Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 Lee & Peggy Wenk ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 23 **************************************** From Heidi.Couture <@t> leica-microsystems.com Fri Aug 12 16:01:50 2011 From: Heidi.Couture <@t> leica-microsystems.com (Heidi.Couture@leica-microsystems.com) Date: Fri Aug 12 16:01:58 2011 Subject: [Histonet] AUTO: Heidi Couture is out of the office. (returning 08/15/2011) Message-ID: I am out of the office until 08/15/2011. I will be out of the office Thursday 8/11 & Friday 8/12, returning Monday 8/15, please call 1800-225-3035 (leica customer service) with any urgent matters, or email lisa.raymond@leica-microsystems.com. Please have your account number and PO available. Thank you, have a great day! Note: This is an automated response to your message "Histonet Digest, Vol 93, Issue 17" sent on 8/12/2011 2:34:53 PM. You will receive a notification for each message you send to this person while the person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Heidi.Couture <@t> leica-microsystems.com Fri Aug 12 16:01:49 2011 From: Heidi.Couture <@t> leica-microsystems.com (Heidi.Couture@leica-microsystems.com) Date: Fri Aug 12 16:02:00 2011 Subject: [Histonet] AUTO: Heidi Couture is out of the office. (returning 08/15/2011) Message-ID: I am out of the office until 08/15/2011. I will be out of the office Thursday 8/11 & Friday 8/12, returning Monday 8/15, please call 1800-225-3035 (leica customer service) with any urgent matters, or email lisa.raymond@leica-microsystems.com. Please have your account number and PO available. Thank you, have a great day! Note: This is an automated response to your message "Histonet Digest, Vol 93, Issue 16" sent on 8/12/2011 11:59:25 AM. You will receive a notification for each message you send to this person while the person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From abijag76 <@t> rediffmail.com Sat Aug 13 03:19:23 2011 From: abijag76 <@t> rediffmail.com (abijag ) Date: Sat Aug 13 03:19:30 2011 Subject: [Histonet] mouse heart trimming and sectioning_papillary muscle Message-ID: <20110813081923.1389.qmail@f6mail-145-158.rediffmail.com> Dear fellow histonetters, I would like to have your suggestions regarding trimming and sectioning of mouse heart. Our pathologists are interested in evaluating mouse ventricle papillary muscle for some lesions. I am not able to cut sections having papillary muscle in all sections.Some sections I am getting but not in all. Anybody experienced this situation and any experience to share. With regards Abi From tahseen <@t> brain.net.pk Sat Aug 13 05:18:58 2011 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Sat Aug 13 05:19:06 2011 Subject: [Histonet] Peloris Rapid tissue processor Message-ID: <22513.203.135.35.66.1313230738.squirrel@brain.net.pk> Dear All, Our lab is in the process of purchasing our automated tissue processor (Peloris Rapid tissue processor). I would really appreciate comments from anyone who really likes, or dislikes the processor that they are using. Thanks in advance!!! Muhammad Tahseen Histology Supervisor Deptt. Pathology Shaukat Khanum Cancer Hospital And Research Center Lahore. Pakistan Ph. +92 42 5180725-36 Ext 2369, Fax. +92 42 5180723 e-mail. Histology <@t> skm.org.pk From rjbuesa <@t> yahoo.com Sat Aug 13 09:19:34 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Aug 13 09:19:39 2011 Subject: [Histonet] Peloris Rapid tissue processor In-Reply-To: <22513.203.135.35.66.1313230738.squirrel@brain.net.pk> Message-ID: <1313245174.8285.YahooMailClassic@web65711.mail.ac4.yahoo.com> Muhammad: The only thing I dislike about the Peloris technology (it is a technology in itself) is that?after the dehydration with 2-propanol, the tissues are subjected to a DRY HOT evaporation of the 2-propanol in vacuum before?the infiltration step with?melted paraffin. That step? of drying out the tissues to eliminate the 2-propanol to "facilitate" the infiltration is the one I do not like because the tissues are exposed to a very high gradient. If you end buying the instrument I think you should run a large series of validation tests to find out if the results you obtain with the Peloris compare with what you are used to, not referring to the sectioning quality of the blocks, but to their microscopic appearance.. Peloris was developed in Australia by VisionBioSystems and later bought by Leica Microsystems. Although there?were tests published by VisionBioSystems, all referred to animal tissues, and the instrument was never independently validated. Leica did not make known (published) independent validations. I for one would never subject the tissues to a hot dry desiccation before infiltration. I hope this will help you in your decision. Ren? J. --- On Sat, 8/13/11, tahseen@brain.net.pk wrote: From: tahseen@brain.net.pk Subject: [Histonet] Peloris Rapid tissue processor To: histonet@lists.utsouthwestern.edu Cc: Histology@skm.org.pk Date: Saturday, August 13, 2011, 6:18 AM Dear All, Our lab is in the process of purchasing our? automated tissue processor (Peloris Rapid tissue processor). I would really appreciate comments from anyone who really likes, or dislikes the processor that they are using. ??? ??? Thanks in advance!!! ??? ??? Muhammad Tahseen ??? ??? Histology Supervisor ??? ?????Deptt. Pathology ??? ??? Shaukat Khanum Cancer Hospital ??? ??? And Research Center Lahore. Pakistan ??? ??? Ph.???+92 42 5180725-36 Ext 2369, ??? ??? Fax. +92 42? 5180723 ??? ??? e-mail. Histology <@t> skm.org.pk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Aug 13 11:37:47 2011 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Aug 13 11:39:24 2011 Subject: [Histonet] Re: IHC on Pig help Message-ID: <11D9615B89C10747B1C985966A63D7CA37ACA7D219@KCL-MAIL04.kclad.ds.kcl.ac.uk> Treat the tissue exactly as if it was human. Sure, your anti CD68 may not work on pig but, it's worth a try. LBH? Probably LDH. If so, better to measure serum levels? Trypen blue? Prob. Trypan blue. It's a dye used for cell viability tests. So, it seems that 2 and 3 are not IHC at all. More details? Also ask for IHC help here, if you wish : http://www.immunoportal.com/ Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From Heidi.Couture <@t> leica-microsystems.com Sat Aug 13 16:02:52 2011 From: Heidi.Couture <@t> leica-microsystems.com (Heidi.Couture@leica-microsystems.com) Date: Sat Aug 13 16:02:59 2011 Subject: [Histonet] AUTO: Heidi Couture is out of the office. (returning 08/15/2011) Message-ID: I am out of the office until 08/15/2011. I will be out of the office Thursday 8/11 & Friday 8/12, returning Monday 8/15, please call 1800-225-3035 (leica customer service) with any urgent matters, or email lisa.raymond@leica-microsystems.com. Please have your account number and PO available. Thank you, have a great day! Note: This is an automated response to your message "Histonet Digest, Vol 93, Issue 18" sent on 8/13/2011 11:59:41 AM. You will receive a notification for each message you send to this person while the person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From shive003 <@t> umn.edu Sat Aug 13 16:42:09 2011 From: shive003 <@t> umn.edu (shive003@umn.edu) Date: Sat Aug 13 16:42:13 2011 Subject: [Histonet] Re: IHC on Pig help In-Reply-To: <11D9615B89C10747B1C985966A63D7CA37ACA7D219@KCL-MAIL04.kclad.ds.kcl.ac.uk> References: <11D9615B89C10747B1C985966A63D7CA37ACA7D219@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: Carl is correct... treat the tissue as if it is human... i.e., use anti-mouse IgG as your link secondary Ab for CD68 mouse primary antibody, etc. Not all CD68s cross-react with non-primates; I can let you know which clones I've tried when I get back into work on Monday. Jan Shivers IHC Section Head Veterinary Diagnostic Lab Univ. of Minnesota College of Veterinary Medicine St. Paul, MN On Aug 13 2011, Hobbs, Carl wrote: > >Treat the tissue exactly as if it was human. > >Sure, your anti CD68 may not work on pig but, it's worth a try. >LBH? Probably LDH. If so, better to measure serum levels? >Trypen blue? Prob. Trypan blue. It's a dye used for cell viability tests. > >So, it seems that 2 and 3 are not IHC at all. > >More details? >Also ask for IHC help here, if you wish : http://www.immunoportal.com/ > > >Carl Hobbs >Histology Manager >Wolfson CARD >School of Biomedical Sciences >Kings College London >Guys Campus >SE1 1UL >Tel: 020 78486813 >Fax: 020 78486816 >020 78486813 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mikoff <@t> pacbell.net Sun Aug 14 03:19:57 2011 From: mikoff <@t> pacbell.net (Keith) Date: Sun Aug 14 03:20:02 2011 Subject: [Histonet] Peloris Rapid tissue processor RE: Histonet Digest, Vol 93, Issue 18, Message: 4 In-Reply-To: <201108131703.p7DH3Cnv021010@flpd110.prodigy.net> Message-ID: <751AAEED784D4334964818781CA0CD51@kitchen> Muhammad: The Peloris technique holds some promise. Although I've never used the system, it received some good, and some bad reviews on the Histonet site as well as other places online, including white papers on the Leica site, itself. I know Leica is selling the Peloris processor, but the papers appear to be legitimate 3d party contributions. You may attempt to contact any of the authors for more information. Below are some of what can be found- Hi. We're currently setting up a histopath lab. We've purchased Peloris tissue processor and have been test processing tissues under different hour protocols. We encountered problems in the 8-hr and 5-hr protocols.Both produced tissues that were too difficult to section in the microtome. My histotechs showed me tissues that were like brittle plastics. Despite reprocessing, the tissues came out the same in the 8-hr protocol. We reprocessed the 5-hr tissues for 12 hrs, the tissues looked microscopically ok. I suspect the tissues were too thickly sampled and poorly fixed. May ask for feedback/advice on the ff;? 1. Possible reasons? 2. Steps to prevent this in the future? 3. Reprocessing protocol in Peloris. Thank you so much. Dr. Evette Demaisip Pathologist,Philippines http://lists.utsouthwestern.edu/pipermail/histonet/2009-September/046556.htm l RE: [Histonet] Peloris tissue processor From: "Ron Martin" We have been using our new Peloris for 5 weeks with great results. So far so good along with good tech support. Steve Westra (Florida rep) and Rick Couture (Mass. tech support) have been great with us. Ron Martin, BS, HTL(ASCP) Histology Supervisor Advanced Dermatology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 05, 2006 12:56 PM To: lhartman18@adelphia.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Peloris tissue processor Run away! Ask them for a list of users. I have one sitting in my storage area waiting on them to pick it up. It has never worked right and the tech support is non existent. The techs do not know how to fix it. It is the second one that they have sent us. If anyone from Vision is reading this please have it removed from our storage area. It has been here for four months waiting on you to pick it up. I hope the acquisition of this company doesn't tarnish the Ventana reputation. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. Source: http://www.histosearch.com/histonet/Sep06/RE.HistonetPeloristissuepA.html The next URL provides a link to the Leica site. At the site Leica provides white papers on the use of the Peloris tissue processor/system: http://www.leica-microsystems.com/products/total-histology/education-series/ whitepaper/ A number of Google links to additional reviews of the processor/procedure are available at: http://www.google.com/#hl=en&cp=14&gs_id=1o&xhr=t&q=Peloris+tissue+processor &pf=p&sclient=psy&source=hp&pbx=1&oq=Peloris+tissue&aq=0&aqi=g1g-v1g-m1&aql= &gs_sm=&gs_upl=&bav=on.2,or.r_gc.r_pw.&fp=116a3ca570808e30&biw=1209&bih=814 My recommendation would be to try the Peloris processor, but to run Best, Keith M. Mikoff, HTL/HT (ASCP) From kiran_g <@t> sbcglobal.net Sun Aug 14 10:32:27 2011 From: kiran_g <@t> sbcglobal.net (kiran_g@sbcglobal.net) Date: Sun Aug 14 10:32:36 2011 Subject: [Histonet] Peloris Rapid tissue processor In-Reply-To: <1313245174.8285.YahooMailClassic@web65711.mail.ac4.yahoo.com> References: <22513.203.135.35.66.1313230738.squirrel@brain.net.pk><1313245174.8285.YahooMailClassic@web65711.mail.ac4.yahoo.com> Message-ID: <1681437290-1313335950-cardhu_decombobulator_blackberry.rim.net-1449916658-@b4.c14.bise6.blackberry> Hi We are currently using Peloris for most of our tissues and its great. Our goal is to move all processing from conventional VIP to Peloris by the end of this year. We validated our own cycle times, not factory installed. The key is size of the tissue and cycle time, if tissues are mixed and cycle time is not sufficient to process, it will cause problems. Thank you, Rene for bringing some good points especially evaporation step, I would definitely look into that. No issues so far and great service! Kiran Kaiser Permanente, CA Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Sender: histonet-bounces@lists.utsouthwestern.edu Date: Sat, 13 Aug 2011 07:19:34 To: ; Cc: Subject: Re: [Histonet] Peloris Rapid tissue processor Muhammad: The only thing I dislike about the Peloris technology (it is a technology in itself) is that?after the dehydration with 2-propanol, the tissues are subjected to a DRY HOT evaporation of the 2-propanol in vacuum before?the infiltration step with?melted paraffin. That step? of drying out the tissues to eliminate the 2-propanol to "facilitate" the infiltration is the one I do not like because the tissues are exposed to a very high gradient. If you end buying the instrument I think you should run a large series of validation tests to find out if the results you obtain with the Peloris compare with what you are used to, not referring to the sectioning quality of the blocks, but to their microscopic appearance.. Peloris was developed in Australia by VisionBioSystems and later bought by Leica Microsystems. Although there?were tests published by VisionBioSystems, all referred to animal tissues, and the instrument was never independently validated. Leica did not make known (published) independent validations. I for one would never subject the tissues to a hot dry desiccation before infiltration. I hope this will help you in your decision. Ren? J. --- On Sat, 8/13/11, tahseen@brain.net.pk wrote: From: tahseen@brain.net.pk Subject: [Histonet] Peloris Rapid tissue processor To: histonet@lists.utsouthwestern.edu Cc: Histology@skm.org.pk Date: Saturday, August 13, 2011, 6:18 AM Dear All, Our lab is in the process of purchasing our? automated tissue processor (Peloris Rapid tissue processor). I would really appreciate comments from anyone who really likes, or dislikes the processor that they are using. ??? ??? Thanks in advance!!! ??? ??? Muhammad Tahseen ??? ??? Histology Supervisor ??? ?????Deptt. Pathology ??? ??? Shaukat Khanum Cancer Hospital ??? ??? And Research Center Lahore. Pakistan ??? ??? Ph.???+92 42 5180725-36 Ext 2369, ??? ??? Fax. +92 42? 5180723 ??? ??? e-mail. Histology <@t> skm.org.pk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heidi.Couture <@t> leica-microsystems.com Sun Aug 14 16:00:51 2011 From: Heidi.Couture <@t> leica-microsystems.com (Heidi.Couture@leica-microsystems.com) Date: Sun Aug 14 16:00:58 2011 Subject: [Histonet] AUTO: Heidi Couture is out of the office. (returning 08/15/2011) Message-ID: I am out of the office until 08/15/2011. I will be out of the office Thursday 8/11 & Friday 8/12, returning Monday 8/15, please call 1800-225-3035 (leica customer service) with any urgent matters, or email lisa.raymond@leica-microsystems.com. Please have your account number and PO available. Thank you, have a great day! Note: This is an automated response to your message "Histonet Digest, Vol 93, Issue 19" sent on 8/14/2011 11:53:09 AM. You will receive a notification for each message you send to this person while the person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From jseaton <@t> wlgore.com Sun Aug 14 18:04:06 2011 From: jseaton <@t> wlgore.com (Janella Seaton) Date: Sun Aug 14 18:04:37 2011 Subject: [Histonet] AUTO: Janella Seaton/WLGORE is out of the office. (returning 08/18/2011) Message-ID: I am out of the office until 08/18/2011. I will respond to your message when I return. Any histology-related requests or questions can be sent to: histology@wlgore.com. Note: This is an automated response to your message "Histonet Digest, Vol 93, Issue 19" sent on 8/14/2011 10:05:13 AM. This is the only notification you will receive while this person is away. From rsrichmond <@t> gmail.com Sun Aug 14 21:23:26 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Aug 14 21:23:30 2011 Subject: [Histonet] Re: GROSSING Message-ID: Kathy Gorham H.T. asks: >>We are looking at getting a pathologist resident for the first time. What can anyone tell me about what we need to allow him to do gross? Do we need a certificate of any kind? Thanks and have a great day.<< A pathology resident had better be able to gross, though you should check - some of them are quite poorly trained in it. The resident may need a state medical license in the state he's working in, and some residents aren't licensed. You should ask your pathologist to check into this. You may need a photocopy of his state license and other credentials for your files. Bob Richmond Samurai Pathologist Knoxville TN From H.J.G.vandeKant <@t> uu.nl Mon Aug 15 07:37:57 2011 From: H.J.G.vandeKant <@t> uu.nl (Kant, H.J.G. van de (Henk)) Date: Mon Aug 15 07:38:06 2011 Subject: [Histonet] cd4 from angio proteomie Message-ID: <8A014D954ADDDD44AC39749D8EB677C903E98B@ICTSC-W-S204.soliscom.uu.nl> Hello, Has somebody used the CD4 antibody from Angio proteomie. It is an anti mouse and it is for paraffin IHC. Catalog # 2H9 Is it working? Henk van de Kant Utrecht Institute of Pharmaceutical Sciences Faculty of Sciences, Utrecht University visiting address: Universiteitsweg 99, room 2.21 3584CG Utrecht, the Netherlands mailing address: PO Box 80.195, 3508TB Utrecht The Netherlands From Caroline.Pratt <@t> uphs.upenn.edu Mon Aug 15 08:16:35 2011 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Mon Aug 15 08:16:45 2011 Subject: [Histonet] Online program for histotechnician program In-Reply-To: <1C03BD76DC9BD14EA266F05C65D173B4430F32FC20@NEIU-SBS.Niu.lan> References: , <1C03BD76DC9BD14EA266F05C65D173B4430F32FC20@NEIU-SBS.Niu.lan> Message-ID: <91FD001DCC6DC84EA6A4B12BB931367801372850@uphmasphi007.UPHS.PENNHEALTH.PRV> We have an HT trained at our site while attending Harford, great end result. :) Caroline M. Pratt, MBA Practice Administrator Dermatopathology Penn Medicine 3020 Market Street, Ste 201 Philadelphia, PA 19104 cell 610-800-1381 phone 215-349-8178 fax 215-662-6150 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Friday, August 12, 2011 2:01 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Online program for histotechnician program I went through the IU program. I'd like to think I'm a swell histotech as a result. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreightl@gmail.com] Sent: Friday, August 12, 2011 1:53 PM To: Kimmie Rabe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Online program for histotechnician program We've had several students go throught the Indiana University program, I believe that the program creats very good histotechs. On Fri, Aug 12, 2011 at 7:00 AM, Kimmie Rabe wrote: > I am exploring the possibilty of recruiting a student to work at our > facility and obtain histology training online (leading to a histotechnician > certificate). I have found programs through the University of North Dakota, > Indiana University School of Medicine, and Harford Community College in > Maryland. > > Does anyone have any experience/insight to offer? > > Kimmie E. Rabe, MD > North Central Pathology, PA > 3701 12th Street North, Suite 201 > St. Cloud, MN 56303 > Phone: 320-253-6554 > Fax: 320-253-1218 > kim@ncpath.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From CThornton <@t> dahlchase.com Mon Aug 15 08:29:45 2011 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon Aug 15 08:29:49 2011 Subject: [Histonet] glass and humidity Message-ID: Has anyone ever done any sort of test/experiment of the effect humidity might have on slides? Last summer we had an issue with inconsistent staining with our IHC and we finally attributed it to the positive charge on the slide being uneven somehow, causing reagents to pool in some areas on the slide and not on others. The issue resolved in October, but has over the past week resurfaced again. We buy our slides in bulk and store them at our courier division, in a non-climate controlled facility. We're beginning to think it's humidity that might be affecting the slides, but we can't say for sure. Does anyone else have any experience with this sort of thing? Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From Luis.Chiriboga <@t> nyumc.org Mon Aug 15 09:41:28 2011 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Mon Aug 15 09:42:37 2011 Subject: [Histonet] pten Antibody Message-ID: Can anyone recommend a pten antibody for use in FFPE? Antibody I was using is no longer available.... Thanks Luis Luis Chiriboga Ph.D OCS Experimental Pathology IHC Core Lab Bellevue Hospital Center Department of Pathology 4w27 (212) 562-4667 Luis.Chiriboga@nyumc.org ------------------------------------------------------------
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================================= 
From coughlin <@t> musc.edu  Mon Aug 15 09:54:08 2011
From: coughlin <@t> musc.edu (Coughlin, Beth)
Date: Mon Aug 15 09:54:03 2011
Subject: [Histonet] LKB 7800B KnifeMaker
Message-ID: 

Does anyone have some quick tips for adjusting a knife maker, specifically a LKB?
We have the guide that came with the equipment. However, it's not very clear (to me) how to adjust the alignment based on what the guide says. It just seems to be an index of sorts without very much advice on how to realign the machine. 
Basically I'm getting knives, even squares, that are not straight on the edge. So, I think the scorer isn't lined up right. But, when I get squares to break, the pins seem to be too far to the edge because there's sometimes a lot of cracking and chipping that's going on too...
The head histologist in the lab says she fixed it, but if anyone could help with general tips and/or guidlines for the future, it would be much appreciated!
Thanks.
From COPPINM <@t> aruplab.com  Mon Aug 15 09:58:11 2011
From: COPPINM <@t> aruplab.com (Coppin, Margaret)
Date: Mon Aug 15 09:58:16 2011
Subject: [Histonet] Toxoplasma control
Message-ID: 

Hello all,

 

Does anyone have a Toxoplasma control they would be willing to share?  I
would even be happy with a few unstained slides if an entire block isn't
available and would be willing to trade control tissue from my lab too. 

 

Thanks in advance.

 

 

Margaret G. Coppin, HT(ASCP)

Technical Supervisor--Immunohistochemistry

 

ARUP Laboratories

500 Chipeta Way

Salt Lake City, UT 84108

(801)583-2787 X3869

coppinm@ARUPLab.com

 

 

 


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Unauthorized forwarding, printing, copying, distributing, or use of
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From settembr <@t> umdnj.edu  Mon Aug 15 11:35:49 2011
From: settembr <@t> umdnj.edu (Settembre, Dana)
Date: Mon Aug 15 11:35:59 2011
Subject: [Histonet] RE: pten Antibody
In-Reply-To: 
References: 
Message-ID: 

Leica sells the NovoCastra line pTEN.  That's what I have been using.
Which vendor have you been using?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis
Sent: Monday, August 15, 2011 10:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] pten Antibody

Can anyone recommend a pten antibody for use in FFPE?  Antibody I was using is no longer available....
Thanks
Luis



Luis Chiriboga Ph.D
OCS Experimental Pathology IHC Core Lab
Bellevue Hospital Center
Department of Pathology 4w27
(212) 562-4667
Luis.Chiriboga@nyumc.org
------------------------------------------------------------
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From liz <@t> premierlab.com  Mon Aug 15 12:43:41 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Mon Aug 15 12:44:28 2011
Subject: [Histonet] mouse heart trimming and sectioning_papillary muscle
In-Reply-To: <20110813081923.1389.qmail@f6mail-145-158.rediffmail.com>
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA075F@SBS2K8.premierlab.local>

You are probably going to have to check unstained sections as you trim into the block.  The heart needs to be grossed in from apex to base in a standard manner, you will need to figure out the best way to do this.  You need to section on the same orientation each time. I'm thinking you need to section in the frontal plane, trim off a small portion of the anterior side of the heart, you can tell the anterior from the posterior by the atria.  The atria are attached to the heart on the posterior side, so you want to trim off the other side, we have done this for rat hearts before to look specifically at the papillary muscle.  I would then section into the block and then pick up unstained slides and look under a microscope to see if the papillary muscle is present.  Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of abijag
Sent: Saturday, August 13, 2011 2:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] mouse heart trimming and sectioning_papillary muscle

Dear fellow histonetters,



I would like to have your suggestions regarding trimming and sectioning of mouse heart. Our pathologists are interested in evaluating mouse ventricle papillary muscle for some lesions. I am not able to cut sections having papillary muscle in all sections.Some sections I am getting but not in all. Anybody experienced this situation and any experience to share.



With regards

Abi
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Histonet@lists.utsouthwestern.edu
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From laurie.colbert <@t> huntingtonhospital.com  Mon Aug 15 13:16:11 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Mon Aug 15 13:16:15 2011
Subject: [Histonet] Cassette Labeler
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AFAE@EXCHANGE3.huntingtonhospital.com>

We currently have a Thermo Fisher Printmate cassette labeler.  I am
looking for something smaller than this to put in an outpatient surgery
center that will be opening soon.  It needs to be able to print the
accession number and a corresponding barcode on the cassettes - and it
needs to be easy enough for our pathologists to use.  Any suggestions?
Vendors welcome to contact me offline.

 

Thanks,

Laurie Colbert

Huntington Hospital

Pasadena, CA 91105

(626) 397-8620

 

From srishan <@t> mail.holyname.org  Mon Aug 15 13:31:48 2011
From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org)
Date: Mon Aug 15 13:32:55 2011
Subject: [Histonet] Cassette Labeler
Message-ID: 

I am looking for  a portable cassette labeler as the cassette printer or 
etcher will have to go through capital budget and so on.  Does anyone know 
about a small cassette labeler which is inexpensive but print the surgical 
numbers well enough to see after processing.
We have tried many pens but the numbers are sometime very fuzzy and takes 
a lot of time to fix this the following day. 

nirmala

__________________

We currently have a Thermo Fisher Printmate cassette labeler.  I am
looking for something smaller than this to put in an outpatient surgery
center that will be opening soon.  It needs to be able to print the
accession number and a corresponding barcode on the cassettes - and it
needs to be easy enough for our pathologists to use.  Any suggestions?
Vendors welcome to contact me offline.



Thanks,

Laurie Colbert

Huntington Hospital

Pasadena, CA 91105

(626) 397-8620



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet








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**** Warning: The information contained in this message is privileged and 
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you are not the intended recipient, you are hereby notified that any 
disclosure, copying, distribution, or taking of any action in reliance on 
the content of this message is strictly prohibited. If you have received 
this communication in error, please notify the sender by replying to this 
message, and then delete it from your system.




From laurie.colbert <@t> huntingtonhospital.com  Mon Aug 15 15:34:54 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Mon Aug 15 15:34:57 2011
Subject: [Histonet] Cytochemistry staining - reference lab
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AFB1@EXCHANGE3.huntingtonhospital.com>

I'm looking for a reference lab in the Los Angeles area that does
cytochemistry stains - specifically a TRAP stain.  Does anyone know
where I could send tissue sections for this stain?

 

Laurie Colbert

(626) 397-8620

From cbarone <@t> NEMOURS.ORG  Mon Aug 15 16:09:35 2011
From: cbarone <@t> NEMOURS.ORG (Barone, Carol )
Date: Mon Aug 15 16:10:31 2011
Subject: [Histonet] protocol for in situ RT_PCR
Message-ID: 

Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5
years (on my Perk and Elmer)...Know there must be some great new
reagents and kits out there...opinions? Send to cbarone@nemours.org. Thx
CB
From micro <@t> superlink.net  Mon Aug 15 20:59:52 2011
From: micro <@t> superlink.net (Markus F. Meyenhofer)
Date: Mon Aug 15 21:00:14 2011
Subject: [Histonet] LKB 7800B KnifeMaker
References: 
Message-ID: <37C6858A1E4B4F4E8A1B464D3DE5D6CE@DJ4VDH31>

It is an alignment problem. Also chipping at the scoring line indicates a 
too high pressure of the scoring wheel onto the glass (there is a set screw 
to loosen the pressure next to the scoring wheel, you have to remove the 
head to adjust). I have an instruction sheet with drawings to align the 
knife maker.
I can send you a copy.
If you still have a problem after alignment, I can recondition and align, 
add a new scoring wheel/pin for a modest charge. Please contact me off line.
Regards,
Markus F. Meyenhofer
Microscopy Labs
Box 338
Red Bank, NJ 07701
732 747 6228


micro@superlink.net
----- Original Message ----- 
From: "Coughlin, Beth" 
To: 
Sent: Monday, August 15, 2011 10:54 AM
Subject: [Histonet] LKB 7800B KnifeMaker


Does anyone have some quick tips for adjusting a knife maker, specifically a 
LKB?
We have the guide that came with the equipment. However, it's not very clear 
(to me) how to adjust the alignment based on what the guide says. It just 
seems to be an index of sorts without very much advice on how to realign the 
machine.
Basically I'm getting knives, even squares, that are not straight on the 
edge. So, I think the scorer isn't lined up right. But, when I get squares 
to break, the pins seem to be too far to the edge because there's sometimes 
a lot of cracking and chipping that's going on too...
The head histologist in the lab says she fixed it, but if anyone could help 
with general tips and/or guidlines for the future, it would be much 
appreciated!
Thanks.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From louise.renton <@t> gmail.com  Tue Aug 16 03:16:35 2011
From: louise.renton <@t> gmail.com (Louise Renton)
Date: Tue Aug 16 03:16:40 2011
Subject: [Histonet] protocol for in situ RT_PCR
In-Reply-To: 
References: 
Message-ID: 

hi could you add me on too pelase?

On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol  wrote:

> Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5
> years (on my Perk and Elmer)...Know there must be some great new
> reagents and kits out there...opinions? Send to cbarone@nemours.org. Thx
> CB
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
From sfonner <@t> labpath.com  Tue Aug 16 05:40:46 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Tue Aug 16 05:44:58 2011
Subject: [Histonet] IHC on Frozen Tissue
Message-ID: <001901cc5c00$f5139b40$df3ad1c0$@com>

Good morning everyone,

 

I was wondering if anyone could help me with a protocol change for the
Ventana Ultra.  I have a pathologist that is requesting an HSVI, HSVll, and
Zoster Virus on frozen tissue that was received for IF studies. I know it's
not Monday, but it is early, and I was hoping for some information about how
to change the protocol for frozen vs. paraffin tissue.  Do I just need to
delete the depar. step and leave everything else the same?  I appreciate any
help you could give.

 

Thanks,

Sheila

 

From sfonner <@t> labpath.com  Tue Aug 16 06:03:50 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Tue Aug 16 06:08:03 2011
Subject: [Histonet] IHC on Frozen Tissue
In-Reply-To: 
References: <001901cc5c00$f5139b40$df3ad1c0$@com>
	
Message-ID: <001e01cc5c04$2e02ecf0$8a08c6d0$@com>

Thank you.


-----Original Message-----
From: Mierow, Brett T. [mailto:Brett.Mierow@essentiahealth.org] 
Sent: Tuesday, August 16, 2011 7:02 AM
To: Sheila Fonner
Subject: RE: [Histonet] IHC on Frozen Tissue

You are correct, leave out the depar. step. 


Brett Mierow, HT (ASCP)
Histology specialist
Essentia Health
SMDC Laboratory
407 East 3rd Street, Duluth, MN 54880 
(218) 786-4510 | brett.mierow@essentiahealth.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila
Fonner
Sent: Tuesday, August 16, 2011 5:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC on Frozen Tissue

Good morning everyone,

 

I was wondering if anyone could help me with a protocol change for the
Ventana Ultra.  I have a pathologist that is requesting an HSVI, HSVll,
and Zoster Virus on frozen tissue that was received for IF studies. I
know it's not Monday, but it is early, and I was hoping for some
information about how to change the protocol for frozen vs. paraffin
tissue.  Do I just need to delete the depar. step and leave everything
else the same?  I appreciate any help you could give.

 

Thanks,

Sheila

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From lynn13361 <@t> aol.com  Tue Aug 16 06:39:29 2011
From: lynn13361 <@t> aol.com (lynn13361@aol.com)
Date: Tue Aug 16 06:39:40 2011
Subject: [Histonet] Re:
Message-ID: <8CE2A204B58E22C-108C-1457@webmail-m003.sysops.aol.com>

I want you to look throw this site! It?s the most interesting stuff I?ve  
seen last time!... http://listadoweb.com/com.page.php?jnohot=49ek4



From sfonner <@t> labpath.com  Tue Aug 16 06:44:19 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Tue Aug 16 06:48:26 2011
Subject: [Histonet] IHC on Frozen Tissue
In-Reply-To: 
References: <001901cc5c00$f5139b40$df3ad1c0$@com>
	
	<001e01cc5c04$2e02ecf0$8a08c6d0$@com>
	
Message-ID: <002a01cc5c09$d5eb9930$81c2cb90$@com>

Does anyone else leave out the cell conditioning step?


-----Original Message-----
From: Yahoo [mailto:kim.tournear@yahoo.com] 
Sent: Tuesday, August 16, 2011 7:41 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue

I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC2. 

Sent from the iPhone of Kim Tournear

On Aug 16, 2011, at 6:03 AM, "Sheila Fonner"  wrote:

> Thank you.
> 
> 
> -----Original Message-----
> From: Mierow, Brett T. [mailto:Brett.Mierow@essentiahealth.org] 
> Sent: Tuesday, August 16, 2011 7:02 AM
> To: Sheila Fonner
> Subject: RE: [Histonet] IHC on Frozen Tissue
> 
> You are correct, leave out the depar. step. 
> 
> 
> Brett Mierow, HT (ASCP)
> Histology specialist
> Essentia Health
> SMDC Laboratory
> 407 East 3rd Street, Duluth, MN 54880 
> (218) 786-4510 | brett.mierow@essentiahealth.org
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila
> Fonner
> Sent: Tuesday, August 16, 2011 5:41 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] IHC on Frozen Tissue
> 
> Good morning everyone,
> 
> 
> 
> I was wondering if anyone could help me with a protocol change for the
> Ventana Ultra.  I have a pathologist that is requesting an HSVI, HSVll,
> and Zoster Virus on frozen tissue that was received for IF studies. I
> know it's not Monday, but it is early, and I was hoping for some
> information about how to change the protocol for frozen vs. paraffin
> tissue.  Do I just need to delete the depar. step and leave everything
> else the same?  I appreciate any help you could give.
> 
> 
> 
> Thanks,
> 
> Sheila
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From SEsparza <@t> seton.org  Tue Aug 16 07:11:34 2011
From: SEsparza <@t> seton.org (Esparza, Sandra)
Date: Tue Aug 16 07:11:38 2011
Subject: [Histonet] IHC on Frozen Tissue
In-Reply-To: <002a01cc5c09$d5eb9930$81c2cb90$@com>
References: <001901cc5c00$f5139b40$df3ad1c0$@com><001e01cc5c04$2e02ecf0$8a08c6d0$@com>
	<002a01cc5c09$d5eb9930$81c2cb90$@com>
Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB041DFE2A@AUSEX2VS1.seton.org>

I leave out the cell conditioning step as well.  

Sandra
Sandra Esparza HT(ASCP)QIHC
Lead Technologist
Dell Children's Medical Center of Central Texas
512-324-0000  x87061
sesparza@seton.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila
Fonner
Sent: Tuesday, August 16, 2011 6:44 AM
To: 'Yahoo'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC on Frozen Tissue

Does anyone else leave out the cell conditioning step?


-----Original Message-----
From: Yahoo [mailto:kim.tournear@yahoo.com] 
Sent: Tuesday, August 16, 2011 7:41 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue

I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC2. 

Sent from the iPhone of Kim Tournear

On Aug 16, 2011, at 6:03 AM, "Sheila Fonner" 
wrote:

> Thank you.
> 
> 
> -----Original Message-----
> From: Mierow, Brett T. [mailto:Brett.Mierow@essentiahealth.org] 
> Sent: Tuesday, August 16, 2011 7:02 AM
> To: Sheila Fonner
> Subject: RE: [Histonet] IHC on Frozen Tissue
> 
> You are correct, leave out the depar. step. 
> 
> 
> Brett Mierow, HT (ASCP)
> Histology specialist
> Essentia Health
> SMDC Laboratory
> 407 East 3rd Street, Duluth, MN 54880 
> (218) 786-4510 | brett.mierow@essentiahealth.org
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila
> Fonner
> Sent: Tuesday, August 16, 2011 5:41 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] IHC on Frozen Tissue
> 
> Good morning everyone,
> 
> 
> 
> I was wondering if anyone could help me with a protocol change for the
> Ventana Ultra.  I have a pathologist that is requesting an HSVI,
HSVll,
> and Zoster Virus on frozen tissue that was received for IF studies. I
> know it's not Monday, but it is early, and I was hoping for some
> information about how to change the protocol for frozen vs. paraffin
> tissue.  Do I just need to delete the depar. step and leave everything
> else the same?  I appreciate any help you could give.
> 
> 
> 
> Thanks,
> 
> Sheila
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From sfonner <@t> labpath.com  Tue Aug 16 07:24:19 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Tue Aug 16 07:28:32 2011
Subject: [Histonet] IHC on Frozen Tissue
In-Reply-To: 
References: <001901cc5c00$f5139b40$df3ad1c0$@com><001e01cc5c04$2e02ecf0$8a08c6d0$@com>
	<002a01cc5c09$d5eb9930$81c2cb90$@com>
	
Message-ID: <002e01cc5c0f$6c1daa10$4458fe30$@com>

Rich,

 

Isn't the cell conditioning (retrieval) mostly done for tissues that have
been in formalin?  If the tissue is fresh (frozen), it has not seen
formalin.  That is the reason I questioned the cell conditioning step.

 

Thank you for your help.

Sheila

 

 

 

From: Richard Yeo [mailto:ryeo@wchosp.org] 
Sent: Tuesday, August 16, 2011 7:54 AM
To: Sheila Fonner
Subject: RE: [Histonet] IHC on Frozen Tissue

 

Sheila,

 

The cell conditioning step is for retrieval. It will depend on what antibody
you are going to run. If you use it for Depar. you will also use it
wiith-out.

 

 

Rich Y

 

  _____  

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sheila Fonner
Sent: Tue 8/16/2011 7:44 AM
To: 'Yahoo'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC on Frozen Tissue

Does anyone else leave out the cell conditioning step?


-----Original Message-----
From: Yahoo [mailto:kim.tournear@yahoo.com]
Sent: Tuesday, August 16, 2011 7:41 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue

I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC2.

Sent from the iPhone of Kim Tournear

On Aug 16, 2011, at 6:03 AM, "Sheila Fonner"  wrote:

> Thank you.
>
>
> -----Original Message-----
> From: Mierow, Brett T. [mailto:Brett.Mierow@essentiahealth.org]
> Sent: Tuesday, August 16, 2011 7:02 AM
> To: Sheila Fonner
> Subject: RE: [Histonet] IHC on Frozen Tissue
>
> You are correct, leave out the depar. step.
>
>
> Brett Mierow, HT (ASCP)
> Histology specialist
> Essentia Health
> SMDC Laboratory
> 407 East 3rd Street, Duluth, MN 54880
> (218) 786-4510 | brett.mierow@essentiahealth.org
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila
> Fonner
> Sent: Tuesday, August 16, 2011 5:41 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] IHC on Frozen Tissue
>
> Good morning everyone,
>
>
>
> I was wondering if anyone could help me with a protocol change for the
> Ventana Ultra.  I have a pathologist that is requesting an HSVI, HSVll,
> and Zoster Virus on frozen tissue that was received for IF studies. I
> know it's not Monday, but it is early, and I was hoping for some
> information about how to change the protocol for frozen vs. paraffin
> tissue.  Do I just need to delete the depar. step and leave everything
> else the same?  I appreciate any help you could give.
>
>
>
> Thanks,
>
> Sheila
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From FLiang <@t> wellstatoc.com  Tue Aug 16 07:45:40 2011
From: FLiang <@t> wellstatoc.com (Liang, Frank)
Date: Tue Aug 16 07:43:55 2011
Subject: [Histonet] protocol for in situ RT_PCR
In-Reply-To: 
References: 
	
Message-ID: 

Hello, 

Does anyone have used alcoholic zinc-formalin to fix mouse tissues? We tried  a few times with this fixative for immunohistochemical staining, it did not work, but when switched back to 4% paraformaldehyde, the immunostaining worked. We like alcoholic zinc-formalin as it gives better morphology. Any suggestions?

Thanks,

Frank  

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton
Sent: Tuesday, August 16, 2011 4:17 AM
To: Barone, Carol; Histonet
Subject: Re: [Histonet] protocol for in situ RT_PCR

hi could you add me on too pelase?

On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol  wrote:

> Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5
> years (on my Perk and Elmer)...Know there must be some great new
> reagents and kits out there...opinions? Send to cbarone@nemours.org. Thx
> CB
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From sfonner <@t> labpath.com  Tue Aug 16 08:24:48 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Tue Aug 16 08:28:55 2011
Subject: [Histonet] IHC on Frozen Tissue
In-Reply-To: 
References: <001901cc5c00$f5139b40$df3ad1c0$@com><001e01cc5c04$2e02ecf0$8a08c6d0$@com><002a01cc5c09$d5eb9930$81c2cb90$@com>
	<002e01cc5c0f$6c1daa10$4458fe30$@com>
	
Message-ID: <004101cc5c17$df13c600$9d3b5200$@com>

Jan,

Thanks, I really appreciate the help.  This is my first attempt, so we'll
see how it turns out. :)

Sheila


-----Original Message-----
From: Jan Shivers [mailto:shive003@umn.edu] 
Sent: Tuesday, August 16, 2011 9:21 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue

Sheila,

On frozens, there should be no need to do tissue conditioning/pretreatment, 
since there are no fixation-caused aldehyde bonds to break.  Bring your 
frozens to room temperature slowly, immerse in your rinse buffer for a few 
minutes, then proceed with your IHC staining protocol.  With frozens, since 
the tissue can at times be more fragile, I always do the H2O2 step AFTER the

primary antibody (but before the HRP-conjugated linking Ab; you don't want 
to quench your substrate enzyme) and I use 0.3% H2O2 instead of 3%.

Jan Shivers
IHC Section Head
UMN Vet Diag Lab

----- Original Message ----- 
From: "Sheila Fonner" 
To: "'Richard Yeo'" ; 
Sent: Tuesday, August 16, 2011 7:24 AM
Subject: RE: [Histonet] IHC on Frozen Tissue


> Rich,
>
>
>
> Isn't the cell conditioning (retrieval) mostly done for tissues that have
> been in formalin?  If the tissue is fresh (frozen), it has not seen
> formalin.  That is the reason I questioned the cell conditioning step.
>
>
>
> Thank you for your help.
>
> Sheila
>
>
>
>
>
>
>
> From: Richard Yeo [mailto:ryeo@wchosp.org]
> Sent: Tuesday, August 16, 2011 7:54 AM
> To: Sheila Fonner
> Subject: RE: [Histonet] IHC on Frozen Tissue
>
>
>
> Sheila,
>
>
>
> The cell conditioning step is for retrieval. It will depend on what 
> antibody
> you are going to run. If you use it for Depar. you will also use it
> wiith-out.
>
>
>
>
>
> Rich Y
>
>
>
>  _____
>
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sheila Fonner
> Sent: Tue 8/16/2011 7:44 AM
> To: 'Yahoo'; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] IHC on Frozen Tissue
>
> Does anyone else leave out the cell conditioning step?
>
>
> -----Original Message-----
> From: Yahoo [mailto:kim.tournear@yahoo.com]
> Sent: Tuesday, August 16, 2011 7:41 AM
> To: Sheila Fonner
> Subject: Re: [Histonet] IHC on Frozen Tissue
>
> I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC2.
>
> Sent from the iPhone of Kim Tournear
>
> On Aug 16, 2011, at 6:03 AM, "Sheila Fonner"  wrote:
>
>> Thank you.
>>
>>
>> -----Original Message-----
>> From: Mierow, Brett T. [mailto:Brett.Mierow@essentiahealth.org]
>> Sent: Tuesday, August 16, 2011 7:02 AM
>> To: Sheila Fonner
>> Subject: RE: [Histonet] IHC on Frozen Tissue
>>
>> You are correct, leave out the depar. step.
>>
>>
>> Brett Mierow, HT (ASCP)
>> Histology specialist
>> Essentia Health
>> SMDC Laboratory
>> 407 East 3rd Street, Duluth, MN 54880
>> (218) 786-4510 | brett.mierow@essentiahealth.org
>>
>> -----Original Message-----
>> From: histonet-bounces@lists.utsouthwestern.edu
>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila
>> Fonner
>> Sent: Tuesday, August 16, 2011 5:41 AM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] IHC on Frozen Tissue
>>
>> Good morning everyone,
>>
>>
>>
>> I was wondering if anyone could help me with a protocol change for the
>> Ventana Ultra.  I have a pathologist that is requesting an HSVI, HSVll,
>> and Zoster Virus on frozen tissue that was received for IF studies. I
>> know it's not Monday, but it is early, and I was hoping for some
>> information about how to change the protocol for frozen vs. paraffin
>> tissue.  Do I just need to delete the depar. step and leave everything
>> else the same?  I appreciate any help you could give.
>>
>>
>>
>> Thanks,
>>
>> Sheila
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> _______________________________________________
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From anonwums1 <@t> gmail.com  Tue Aug 16 08:31:59 2011
From: anonwums1 <@t> gmail.com (Adam .)
Date: Tue Aug 16 08:32:05 2011
Subject: [Histonet] protocol for in situ RT_PCR
In-Reply-To: 
References: 
	
	
Message-ID: 

I've used 10% zinc buffered formalin from Anatech (no alcohol) on mouse
bones with good results. It's possible that the alcohol is denaturing your
antigens of interest. Is there a reason you need to use alcoholic formalin?

Adam

On Tue, Aug 16, 2011 at 7:45 AM, Liang, Frank  wrote:

> Hello,
>
> Does anyone have used alcoholic zinc-formalin to fix mouse tissues? We
> tried  a few times with this fixative for immunohistochemical staining, it
> did not work, but when switched back to 4% paraformaldehyde, the
> immunostaining worked. We like alcoholic zinc-formalin as it gives better
> morphology. Any suggestions?
>
> Thanks,
>
> Frank
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton
> Sent: Tuesday, August 16, 2011 4:17 AM
> To: Barone, Carol; Histonet
> Subject: Re: [Histonet] protocol for in situ RT_PCR
>
> hi could you add me on too pelase?
>
> On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol 
> wrote:
>
> > Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5
> > years (on my Perk and Elmer)...Know there must be some great new
> > reagents and kits out there...opinions? Send to cbarone@nemours.org. Thx
> > CB
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
> "There are nights when the wolves are silent and only the moon howls".
> George Carlin
> No trees were killed in the sending of this message.
> However, many electrons were terribly inconvenienced.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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>
> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
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>
From dreynold <@t> mdanderson.org  Tue Aug 16 09:13:06 2011
From: dreynold <@t> mdanderson.org (Reynolds,Donna M)
Date: Tue Aug 16 09:14:00 2011
Subject: [Histonet] H&E on Frozen
In-Reply-To: <99e5a5fa-e12f-4b86-bb1a-eb18d8685347@DCPWPRTR02.mdanderson.edu>
References: <99e5a5fa-e12f-4b86-bb1a-eb18d8685347@DCPWPRTR02.mdanderson.edu>
Message-ID: <785BBF0C5F49CE41BA74460A43A08F022EA51EA848@DCPWVMBXC0VS3.mdanderson.edu>

We do this frequently. 
Fix slides in Methanol (45ml) + formaldehyde (5ml) for 10 minutes. 
Wash several changes of tap water
Stain with Harris Hematoxylin for 3 minutes
Proceed as you would for paraffin  sections
Get great staining. 
I am sure if you are using a different Hematoxylin you could use it the same way you use it for paraffin

Donna Reynolds HT(ASCP)
Chief Histology Technician, Core Immuno Lab
U. T. M.D. Anderson Cancer Center, Houston, TX
713-792-8106
e-mail dreynold@mdanderson.org

Message: 2
Date: Fri, 12 Aug 2011 08:52:11 -0700 (PDT)
From: Jennifer Sipes 
Subject: [Histonet] H&E for Frozen Tissue
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<1313164331.88113.YahooMailNeo@web125406.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Does anyone have a good protocol for staining frozen sections with H&E?? Also, any mounting medium suggestions?? This is the first time I've been asked to do this for frozen. 
?
Thanks a bunch everyone!
Jen
?
Jennifer K. Sipes, ALAT 
Sr. Laboratory Technician 
Johns Hopkins University 
Ross 933 
720 Rutland Avenue 
Baltimore, MD? 21205 
phone:???? 410-614-0131 
fax:???????? 410-955-9677 
cell:???????? 443-631-6361 
e-mail:? jsipes1@jhmi.edu 

------------------------------

**

From rjbuesa <@t> yahoo.com  Tue Aug 16 09:19:49 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Aug 16 09:19:58 2011
Subject: [Histonet] Peloris Rapid tissue processor
Message-ID: <1313504389.28053.YahooMailClassic@web65706.mail.ac4.yahoo.com>

Dear Colleagues:
I have just received the following e-mail from Leica Microsystems.
It is evident that I was wrong and that now the tissues in the Peloris instrument?go directly from the 2-propanol to the parafin wax and are not air dried.
I just wanted to share this?correction with you.
Ren? J.?

--- On Mon, 8/15/11, Mary.Cheles@leica-microsystems.com  wrote:


From: Mary.Cheles@leica-microsystems.com 
Subject: Re: [Histonet] Peloris Rapid tissue processor
To: "Rene J Buesa" 
Date: Monday, August 15, 2011, 7:03 PM


Dear Rene,

I read your statement on Peloris today. It is certainly not our intent to
try to censor the information placed on Histonet. However, I would like to
point out that there is some inaccuracy in your assessment.

The? inaccuracy is in the statement that tissues are subjected to "DRY HOT
evaporation.....". This is simply not true. Within the xylene-free
protocols of the instrument, isopropyl does act as a clearant instead of
xylene. Therefore, tissue transitions from isopropyl directly into
paraffin. The temperature of the 1st two paraffins are elevated to
"evaporate" the isopropyl but the tissue is physically in paraffin
undergoing infiltration during this simultaneous process.

If you have any questions, we would be happy to answer them. I am hopeful
that you will see fit to write in to Histonet with a clarifying statement.

We would be happy to send you white papers on the Peloris technology if you
are interested in receiving them.

Best Regards,

Mary Cheles, MPH, HTL, DLM (ASCP)
Director, Technical Services & Education Programs
Leica Microsystems
Biosystems Division
1700 Leider Lane
Buffalo Grove, IL 60089
847.317.5916 Office
847.236.3058 Fax
www.leica-microsystems.com



? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???
? ? ? ? ? ???Rene J Buesa? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
? ? ? ? ? ???? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???To 
? ? ? ? ? ???Sent by:? ? ? ? ? ? ? ? ? histonet@lists.utsouthwestern.edu,? 
? ? ? ? ? ???histonet-bounces@? ? ? ???tahseen@brain.net.pk? ? ? ? ? ? ? ? 
? ? ? ? ? ???lists.utsouthwest? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? cc 
? ? ? ? ? ???ern.edu? ? ? ? ? ? ? ? ???Histology@skm.org.pk? ? ? ? ? ? ? ? 
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???Subject 
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???Re: [Histonet] Peloris Rapid tissue 
? ? ? ? ? ???08/13/2011 09:19? ? ? ? ? processor? ? ? ? ? ? ? ? ? ? ? ? ???
? ? ? ? ? ???AM? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???




Muhammad:
The only thing I dislike about the Peloris technology (it is a technology
in itself) is that?after the dehydration with 2-propanol, the tissues are
subjected to a DRY HOT evaporation of the 2-propanol in vacuum before?the
infiltration step with?melted paraffin.
That step? of drying out the tissues to eliminate the 2-propanol to
"facilitate" the infiltration is the one I do not like because the tissues
are exposed to a very high gradient.
If you end buying the instrument I think you should run a large series of
validation tests to find out if the results you obtain with the Peloris
compare with what you are used to, not referring to the sectioning quality
of the blocks, but to their microscopic appearance..
Peloris was developed in Australia by VisionBioSystems and later bought by
Leica Microsystems.
Although there?were tests published by VisionBioSystems, all referred to
animal tissues, and the instrument was never independently validated. Leica
did not make known (published) independent validations.
I for one would never subject the tissues to a hot dry desiccation before
infiltration.
I hope this will help you in your decision.
Ren? J.
--- On Sat, 8/13/11, tahseen@brain.net.pk  wrote:


From: tahseen@brain.net.pk 
Subject: [Histonet] Peloris Rapid tissue processor
To: histonet@lists.utsouthwestern.edu
Cc: Histology@skm.org.pk
Date: Saturday, August 13, 2011, 6:18 AM



Dear All,
Our lab is in the process of purchasing our? automated tissue processor
(Peloris Rapid tissue processor).
I would really appreciate comments from anyone who really likes, or
dislikes the processor that they are using.

??? ??? Thanks in advance!!!

??? ??? Muhammad Tahseen
??? ??? Histology Supervisor
??? ?????Deptt. Pathology
??? ??? Shaukat Khanum Cancer Hospital
??? ??? And Research Center Lahore. Pakistan
??? ??? Ph.???+92 42 5180725-36 Ext 2369,
??? ??? Fax. +92 42? 5180723
??? ??? e-mail. Histology <@t> skm.org.pk



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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Histonet@lists.utsouthwestern.edu
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From COPPINM <@t> aruplab.com  Tue Aug 16 09:21:20 2011
From: COPPINM <@t> aruplab.com (Coppin, Margaret)
Date: Tue Aug 16 09:21:35 2011
Subject: [Histonet] Toxoplasma Control
Message-ID: 

Hello everyone,

 

Thanks for the response regarding the Toxoplasma control.  It's nice to
have such a supportive network available for these types of needs.

 

Margaret

 

Margaret G. Coppin, HT(ASCP)

Technical Supervisor--Immunohistochemistry

 

ARUP Laboratories

500 Chipeta Way

Salt Lake City, UT 84108

(801)583-2787 X3869

coppinm@ARUPLab.com

 

 

 


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attachments are from ARUP Laboratories and are intended only for the
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and may constitute inside or non-public information under
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Unauthorized forwarding, printing, copying, distributing, or use of
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Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1
(800) 522-2787 ext. 2100
From NLEMKE1 <@t> hfhs.org  Tue Aug 16 12:13:19 2011
From: NLEMKE1 <@t> hfhs.org (Lemke, Nancy)
Date: Tue Aug 16 12:13:32 2011
Subject: [Histonet] use of isopentane substitutes for tissue freezing
Message-ID: <66EF8D68E063C044B9AC46E99E3502BF03D38644@RHWMBX01.corp.ds.hfhs.org>


Hello all,
I am considering purchasing a freezing unit through Thermo that can be used with either isopentane of a new solution, HFE-7000 that is sold with the unit.  I need to be able to have frozen samples that allow high quality DNA and RNA extracted.  Does anyone have any experience with this fluid and molecular analytes?
Thanks very much,

Nancy W Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
rm 3115 E&R Bldg
2799 W Grand Blvd
Detroit, MI 48202
(313) 916-8648  phone
(313) 916-9855  fax


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From wanglianebox <@t> yahoo.com.cn  Tue Aug 16 12:14:25 2011
From: wanglianebox <@t> yahoo.com.cn (=?utf-8?B?6L+eIOeOiw==?=)
Date: Tue Aug 16 12:14:32 2011
Subject: [Histonet] Details about Masson Trichrome Stain !
Message-ID: <1313514865.2947.YahooMailClassic@web15707.mail.cnb.yahoo.com>

Details about Masson Trichrome Stain !
?
Biebrich Scarlet:
Biebrich scarlet 2.7 gm
Acid fuchsin 0.3 gm
Distilled water 300.0 ml
Glacial acetic acid 3.0 ml
?
I am not sure what the Biebrich Scarlet here is ? Acid Red 66 or acid Red 73 ?
?
Acid Red 73 http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=49823|FLUKA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
?
Acid Red 66 http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=B6008|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
?
What is the main difference between these two? 
May I choose 66, 73 or anyone?
?
Thanks !
?
Wang Lian, MD?
Medical School?of Nanjing?University
Hankou Road 22#
Nanjing, Jiangsu Province, China
e-mail: wanglianebox@yahoo.com.cn

?
?
From Tammy.Pickles <@t> inspection.gc.ca  Tue Aug 16 12:16:17 2011
From: Tammy.Pickles <@t> inspection.gc.ca (Tammy Pickles)
Date: Tue Aug 16 12:16:27 2011
Subject: [Histonet] Scorched tissue
Message-ID: <4E4A5180.B440.007F.1@inspection.gc.ca>

Hi,
 
I am wondering if anyone can help me out with some processing issues that our lab has been having. We recently have been having reagent contamination issues with alcohol and xylene, where our tissues are soft and cloudy after processing. It seems that the reagent valves on the processor were not operating properly. We changed all reagents after each processing run (we were waiting for replacement parts and needed to continue processing) this seemed to work but then we noticed an additional artifact. The outer cell layer (all tissue types) appears scorched, this does not appear throughout the tissue only on the outer edge. 
 
I am wondering if anyone else has seen this artifact and how they resolved the issue? Any comments or advice are greatly appreciated!
 
Tammy
From JWeems <@t> sjha.org  Tue Aug 16 12:23:00 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Tue Aug 16 12:23:05 2011
Subject: [Histonet] Details about Masson Trichrome Stain !
In-Reply-To: <1313514865.2947.YahooMailClassic@web15707.mail.cnb.yahoo.com>
References: <1313514865.2947.YahooMailClassic@web15707.mail.cnb.yahoo.com>
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408277BDF87@CHEXCMS10.one.ads.che.org>

 
http://stainsfile.info/StainsFile/dyes/26905.htm

This says 66...



Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ? ?
Sent: Tuesday, August 16, 2011 13:14
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Details about Masson Trichrome Stain !

Details about Masson Trichrome Stain !
?
Biebrich Scarlet:
Biebrich scarlet 2.7 gm
Acid fuchsin 0.3 gm
Distilled water 300.0 ml
Glacial acetic acid 3.0 ml
?
I am not sure what the Biebrich Scarlet here is ? Acid Red 66 or acid Red 73 ?
?
Acid Red 73 http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=49823|FLUKA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
?
Acid Red 66 http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=B6008|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
?
What is the main difference between these two? 
May I choose 66, 73 or anyone?
?
Thanks !
?
Wang Lian, MD
Medical School?of Nanjing?University
Hankou Road 22#
Nanjing, Jiangsu Province, China
e-mail: wanglianebox@yahoo.com.cn

?
?
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Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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It may contain information that is privileged and 
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From bennett777 <@t> gmail.com  Tue Aug 16 13:48:51 2011
From: bennett777 <@t> gmail.com (Kevin Bennett)
Date: Tue Aug 16 13:48:58 2011
Subject: [Histonet] Aqueous mounting media
Message-ID: 

All,
Can anyone recommend a quality aqueous mounting media? I was using Aquatex
from EMD chemicals but it's been discontinued, tried Aqua-Mount from Thermo
but show air bubbles a few days after coverslipping.

Thanks,
Kevin Bennett HT
From trathborne <@t> somerset-healthcare.com  Tue Aug 16 13:53:22 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Tue Aug 16 13:53:33 2011
Subject: [Histonet] RE: H&E on Frozen
In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F022EA51EA848@DCPWVMBXC0VS3.mdanderson.edu>
References: <99e5a5fa-e12f-4b86-bb1a-eb18d8685347@DCPWPRTR02.mdanderson.edu>
	<785BBF0C5F49CE41BA74460A43A08F022EA51EA848@DCPWVMBXC0VS3.mdanderson.edu>
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F51A54@SMCMAIL01.somerset-healthcare.com>

Our protocol is to:

Use a positively charged slide
Fix in 10% formalin for 1 minute
Rinse in tap (a few dips)
Gill 3 for 1 minute
Rinse in tap (a few dips)
3-5 dips in bluing
Rinse in tap (a few dips)
Eosin 2-3 dips
100% ETOH (a few dips)
100% ETOH (a few dips)
Xylene (dip until clear)
Mount with Richard- Allan Mounting Medium

After the Gill3, Eosin, and last alcohol, the back of the slide is wiped clean of excess solution to prevent carryover.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M
Sent: Tuesday, August 16, 2011 10:13 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] H&E on Frozen

We do this frequently. 
Fix slides in Methanol (45ml) + formaldehyde (5ml) for 10 minutes. 
Wash several changes of tap water
Stain with Harris Hematoxylin for 3 minutes Proceed as you would for paraffin  sections Get great staining. 
I am sure if you are using a different Hematoxylin you could use it the same way you use it for paraffin

Donna Reynolds HT(ASCP)
Chief Histology Technician, Core Immuno Lab U. T. M.D. Anderson Cancer Center, Houston, TX
713-792-8106
e-mail dreynold@mdanderson.org

Message: 2
Date: Fri, 12 Aug 2011 08:52:11 -0700 (PDT)
From: Jennifer Sipes 
Subject: [Histonet] H&E for Frozen Tissue
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<1313164331.88113.YahooMailNeo@web125406.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Does anyone have a good protocol for staining frozen sections with H&E?? Also, any mounting medium suggestions?? This is the first time I've been asked to do this for frozen. 
?
Thanks a bunch everyone!
Jen
?
Jennifer K. Sipes, ALAT
Sr. Laboratory Technician
Johns Hopkins University
Ross 933
720 Rutland Avenue
Baltimore, MD? 21205
phone:???? 410-614-0131
fax:???????? 410-955-9677
cell:???????? 443-631-6361
e-mail:? jsipes1@jhmi.edu 

------------------------------

**

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From Loralee_Mcmahon <@t> URMC.Rochester.edu  Tue Aug 16 14:03:27 2011
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Tue Aug 16 14:04:38 2011
Subject: [Histonet] Aqueous mounting media
In-Reply-To: 
References: 
Message-ID: 

We have been using an aqueous mounting media from Diagnostic Biosystems. 
 http://dbiosys.com/

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kevin Bennett [bennett777@gmail.com]
Sent: Tuesday, August 16, 2011 2:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Aqueous mounting media

All,
Can anyone recommend a quality aqueous mounting media? I was using Aquatex
from EMD chemicals but it's been discontinued, tried Aqua-Mount from Thermo
but show air bubbles a few days after coverslipping.

Thanks,
Kevin Bennett HT
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From Montina.VanMeter <@t> pbrc.edu  Tue Aug 16 14:05:22 2011
From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter)
Date: Tue Aug 16 14:05:27 2011
Subject: [Histonet] Aqueous mounting media
In-Reply-To: 
References: 
Message-ID: 

ProLong Gold (Invitrogen)






Montina J. Van Meter, HT (ASCP)
Lab Manager
Dept. of Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kevin
Bennett
Sent: Tuesday, August 16, 2011 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Aqueous mounting media

All,
Can anyone recommend a quality aqueous mounting media? I was using
Aquatex from EMD chemicals but it's been discontinued, tried Aqua-Mount
from Thermo but show air bubbles a few days after coverslipping.

Thanks,
Kevin Bennett HT
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From lldewe <@t> gmail.com  Tue Aug 16 15:14:23 2011
From: lldewe <@t> gmail.com (Loralei Dewe)
Date: Tue Aug 16 15:14:26 2011
Subject: [Histonet] Aqueous mounting media
In-Reply-To: 
References: 
	
Message-ID: 

Probe mount aqueous based mounting media from Innovex. My personal
favorite!!

Loralei Dewe



On Tue, Aug 16, 2011 at 12:05 PM, Montina Van Meter <
Montina.VanMeter@pbrc.edu> wrote:

> ProLong Gold (Invitrogen)
>
>
>
>
>
>
> Montina J. Van Meter, HT (ASCP)
> Lab Manager
> Dept. of Autonomic Neuroscience
> Pennington Biomedical Research Center
> 6400 Perkins Rd.
> Baton Rouge, LA  70791
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kevin
> Bennett
> Sent: Tuesday, August 16, 2011 1:49 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Aqueous mounting media
>
>  All,
> Can anyone recommend a quality aqueous mounting media? I was using
> Aquatex from EMD chemicals but it's been discontinued, tried Aqua-Mount
> from Thermo but show air bubbles a few days after coverslipping.
>
> Thanks,
> Kevin Bennett HT
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From techonebs <@t> comcast.net  Tue Aug 16 18:29:42 2011
From: techonebs <@t> comcast.net (Matt Mincer)
Date: Tue Aug 16 18:28:00 2011
Subject: [Histonet] Art Challenge
Message-ID: <4E4AFD66.9030004@comcast.net>

Hey All,

I have been working on updating the look of our website and booth for 
NSH. The graphic design people I am working with don't seem to be 
getting the look I am going for. This gave me an idea. I would like to 
challenge all of the crafty Histoneters to draw, paint or Photoshop (or 
whatever your preferred medium is) a cartoon, painting or caricature of 
histology equipment. The winner will receive a gift and my unending 
gratitude.

Thanks

-- 
Matthew Mincer
Tech One Biomedical Services
159 N Marion Street, PMB163
Oak Park, IL 60301
(708) 383-6040 X 10
fax (708) 383-6045
cell (708) 822-3738


From talulahgosh <@t> gmail.com  Tue Aug 16 20:33:22 2011
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Tue Aug 16 20:33:25 2011
Subject: [Histonet] RE: H&E on Frozen
In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F51A54@SMCMAIL01.somerset-healthcare.com>
References: <99e5a5fa-e12f-4b86-bb1a-eb18d8685347@DCPWPRTR02.mdanderson.edu>
	<785BBF0C5F49CE41BA74460A43A08F022EA51EA848@DCPWVMBXC0VS3.mdanderson.edu>
	<3AD061FE740D464FAC7BF6B5CFB7570711F51A54@SMCMAIL01.somerset-healthcare.com>
Message-ID: 

I concur on this.
If my boss will let me, I can send you our elaborate protocol for H&E
staining,  It involves many dishes and many washes.  But your H&E will be
perfect.
I don't have the protocol with me right now,as I'm at home but tomorrow I
will ask about sending you the protocol.  I don't see why we couldn't send
you ours, but I'm going to ask just in case.

A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Tue, Aug 16, 2011 at 2:53 PM, Rathborne, Toni <
trathborne@somerset-healthcare.com> wrote:

> Our protocol is to:
>
> Use a positively charged slide
> Fix in 10% formalin for 1 minute
> Rinse in tap (a few dips)
> Gill 3 for 1 minute
> Rinse in tap (a few dips)
> 3-5 dips in bluing
> Rinse in tap (a few dips)
> Eosin 2-3 dips
> 100% ETOH (a few dips)
> 100% ETOH (a few dips)
> Xylene (dip until clear)
> Mount with Richard- Allan Mounting Medium
>
> After the Gill3, Eosin, and last alcohol, the back of the slide is wiped
> clean of excess solution to prevent carryover.
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M
> Sent: Tuesday, August 16, 2011 10:13 AM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: [Histonet] H&E on Frozen
>
> We do this frequently.
> Fix slides in Methanol (45ml) + formaldehyde (5ml) for 10 minutes.
> Wash several changes of tap water
> Stain with Harris Hematoxylin for 3 minutes Proceed as you would for
> paraffin  sections Get great staining.
> I am sure if you are using a different Hematoxylin you could use it the
> same way you use it for paraffin
>
> Donna Reynolds HT(ASCP)
> Chief Histology Technician, Core Immuno Lab U. T. M.D. Anderson Cancer
> Center, Houston, TX
> 713-792-8106
> e-mail dreynold@mdanderson.org
>
> Message: 2
> Date: Fri, 12 Aug 2011 08:52:11 -0700 (PDT)
> From: Jennifer Sipes 
> Subject: [Histonet] H&E for Frozen Tissue
> To: "histonet@lists.utsouthwestern.edu"
>        
> Message-ID:
>        <1313164331.88113.YahooMailNeo@web125406.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Does anyone have a good protocol for staining frozen sections with H&E??
> Also, any mounting medium suggestions?? This is the first time I've been
> asked to do this for frozen.
> ?
> Thanks a bunch everyone!
> Jen
> ?
> Jennifer K. Sipes, ALAT
> Sr. Laboratory Technician
> Johns Hopkins University
> Ross 933
> 720 Rutland Avenue
> Baltimore, MD? 21205
> phone:???? 410-614-0131
> fax:???????? 410-955-9677
> cell:???????? 443-631-6361
> e-mail:? jsipes1@jhmi.edu
>
> ------------------------------
>
> **
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical Center
> and are intended only for the addressee.  The information contained in this
> message is confidential and may contain privileged, confidential,
> proprietary and/or trade secret information entitled to protection and/or
> exemption from disclosure under applicable law.  Unauthorized forwarding,
> printing, copying, distribution, or use of such information is strictly
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From talulahgosh <@t> gmail.com  Tue Aug 16 20:40:38 2011
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Tue Aug 16 20:40:42 2011
Subject: [Histonet] Aqueous mounting media
In-Reply-To: 
References: 
Message-ID: 

We use Gel Mount


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Tue, Aug 16, 2011 at 2:48 PM, Kevin Bennett  wrote:

> All,
> Can anyone recommend a quality aqueous mounting media? I was using Aquatex
> from EMD chemicals but it's been discontinued, tried Aqua-Mount from Thermo
> but show air bubbles a few days after coverslipping.
>
> Thanks,
> Kevin Bennett HT
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From talulahgosh <@t> gmail.com  Tue Aug 16 20:45:44 2011
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Tue Aug 16 20:45:48 2011
Subject: [Histonet] Aqueous mounting media
In-Reply-To: 
References: 
	
Message-ID: 

Sorry let me send that again with a link
*http://tinyurl.com/3uw5lcm
*I have no idea why we use this particular medium.  *
*
A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Tue, Aug 16, 2011 at 9:40 PM, Emily Sours  wrote:

> We use Gel Mount
>
>
> A great book should leave you with many experiences, and slightly
> exhausted. You should live several lives while reading it.
> -William Styron
>
>
>
> On Tue, Aug 16, 2011 at 2:48 PM, Kevin Bennett wrote:
>
>> All,
>> Can anyone recommend a quality aqueous mounting media? I was using Aquatex
>> from EMD chemicals but it's been discontinued, tried Aqua-Mount from
>> Thermo
>> but show air bubbles a few days after coverslipping.
>>
>> Thanks,
>> Kevin Bennett HT
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
From micro <@t> superlink.net  Tue Aug 16 21:02:28 2011
From: micro <@t> superlink.net (Markus F. Meyenhofer)
Date: Tue Aug 16 21:02:50 2011
Subject: [Histonet] Aqueous mounting media
References: 
Message-ID: 

The un-coupled Naphthol salts (from the phosphatase reaction, dissolved in
the lipids of the tissue) degrade and cause  N2 bubbles .
We solved this problem (in the 60ties!)with a post treatment after the
reaction:
After the reaction, rinse in dist water,
post fix in formalin 1:4 parts water (just dist. water) for 15 min.
(re-usable), Rinse in dist water and post-treat in a solution of 1%
Sulfanilic acid in 10% Acetic acid (re-usable) for 15 min., rinse in dist
water 4x and mount.
(we used at that time glycerin jelly and/or Aquamount)

Regards,
Markus F. Meyenhofer
Microscopy Labs
Box 338
Red Bank, NJ 07701
732 747 6228
micro@superlink.net


----- Original Message ----- 
From: "Kevin Bennett" 
To: 
Sent: Tuesday, August 16, 2011 2:48 PM
Subject: [Histonet] Aqueous mounting media


> All,
> Can anyone recommend a quality aqueous mounting media? I was using Aquatex
> from EMD chemicals but it's been discontinued, tried Aqua-Mount from 
> Thermo
> but show air bubbles a few days after coverslipping.
>
> Thanks,
> Kevin Bennett HT
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From cpyse <@t> x-celllab.com  Wed Aug 17 07:01:51 2011
From: cpyse <@t> x-celllab.com (Cynthia Pyse)
Date: Wed Aug 17 07:02:07 2011
Subject: [Histonet] unstained slides
Message-ID: <000301cc5cd5$738b9a20$5aa2ce60$@com>

Hello Histonetters,

 

Recently we have seen an increase number of unstained slides requested from
outside facilities. We do not release our blocks, so unstained slides are
the only option for any facility to obtain our tissue. Currently we do not
charge for these slides, but I am rethinking that policy. What is everyone
doing about unstained slide requests? Are you charging for the slides? How
much per slide?  Any information would be helpful. Thanks in advance.

Cindy

 

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory/Histology Supervisor

X-Cell Laboratories

716-250-9235 Ext. 232

e-mail cpyse@x-celllab.com

 

 

From LRaff <@t> uropartners.com  Wed Aug 17 07:39:00 2011
From: LRaff <@t> uropartners.com (Lester Raff MD)
Date: Wed Aug 17 07:39:06 2011
Subject: [Histonet] unstained slides
In-Reply-To: <000301cc5cd5$738b9a20$5aa2ce60$@com>
References: <000301cc5cd5$738b9a20$5aa2ce60$@com>
Message-ID: 

Cindy:

If these outside facilities are commercial labs doing proprietary
testing, many of them will pay a set fee to your laboratory for
preparing the slides.  This needs to be negotiated with the commercial
lab rep, but may be in the $40-50 range

Lester J. Raff, MD
Medical Director
UroPartners Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel 708.486.0076
Fax 708.492.0203
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia
Pyse
Sent: Wednesday, August 17, 2011 7:02 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] unstained slides

Hello Histonetters,

 

Recently we have seen an increase number of unstained slides requested
from
outside facilities. We do not release our blocks, so unstained slides
are
the only option for any facility to obtain our tissue. Currently we do
not
charge for these slides, but I am rethinking that policy. What is
everyone
doing about unstained slide requests? Are you charging for the slides?
How
much per slide?  Any information would be helpful. Thanks in advance.

Cindy

 

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory/Histology Supervisor

X-Cell Laboratories

716-250-9235 Ext. 232

e-mail cpyse@x-celllab.com

 

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From abuchiane <@t> bmhvt.org  Wed Aug 17 08:42:54 2011
From: abuchiane <@t> bmhvt.org (Anita Buchiane)
Date: Wed Aug 17 08:42:59 2011
Subject: [Histonet] Creative Waste Solutions
Message-ID: <602863D272B56749A70CBA315D7DC702062A6216@bmhexch.bmhvt.org>

Does anyone know of a distributor of Creative Waste Solutions products?


_______________________________________________________________

The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email.
_______________________________________________________________
From 41dmb41 <@t> gmail.com  Wed Aug 17 09:01:50 2011
From: 41dmb41 <@t> gmail.com (Drew Meyer)
Date: Wed Aug 17 08:59:49 2011
Subject: [Histonet] Creative Waste Solutions
In-Reply-To: <602863D272B56749A70CBA315D7DC702062A6216@bmhexch.bmhvt.org>
References: <602863D272B56749A70CBA315D7DC702062A6216@bmhexch.bmhvt.org>
Message-ID: <2A4A4354-B7A1-4CAD-8C06-82385127FFBA@gmail.com>

I may be wrong about this, but I don't think Rex has anyone else distributing his stuff yet.  However, some of the stuff they sell is merely products that they relabel to distribute themselves.  So, it depends on what specific products you're talking about. 

Drew

Sent from my iPad

On Aug 17, 2011, at 9:42 AM, Anita Buchiane  wrote:

> Does anyone know of a distributor of Creative Waste Solutions products?
> 
> 
> _______________________________________________________________
> 
> The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email.
> _______________________________________________________________
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From MSHERWOOD <@t> PARTNERS.ORG  Wed Aug 17 09:06:52 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Wed Aug 17 09:07:02 2011
Subject: [Histonet] Creative Waste Solutions
In-Reply-To: <602863D272B56749A70CBA315D7DC702062A6216@bmhexch.bmhvt.org>
References: <602863D272B56749A70CBA315D7DC702062A6216@bmhexch.bmhvt.org>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB594B@PHSXMB30.partners.org>

They sell there own supplies.  We were just introduced to them by an independent
salesperson. 


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane
Sent: Wednesday, August 17, 2011 9:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Creative Waste Solutions

Does anyone know of a distributor of Creative Waste Solutions products?


_______________________________________________________________



The information contained in, or attached to, this e-mail, may contain
confidential information and is intended solely for the use of the individual or
entity to whom it is addressed and may be subject to legal privilege. If you
have received this e-mail in error you should notify the sender immediately by
reply e-mail, delete the message from your system and notify your system
manager. Please do not copy it for any purpose, or disclose its contents to any
other person. The views or opinions presented in this e-mail are solely those of
the author and do not necessarily represent those of the company. The recipient
should check this e-mail and any attachments for the presence of viruses. The
company accepts no liability for any damage caused, directly or indirectly, by
any virus transmitted in this email.

_______________________________________________________________
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From abuchiane <@t> bmhvt.org  Wed Aug 17 09:14:47 2011
From: abuchiane <@t> bmhvt.org (Anita Buchiane)
Date: Wed Aug 17 09:14:51 2011
Subject: [Histonet] Creative Waste Solutions
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB594B@PHSXMB30.partners.org>
References: <602863D272B56749A70CBA315D7DC702062A6216@bmhexch.bmhvt.org>
	<073AE2BEA1C2BA4A8837AB6C4B943D9708DB594B@PHSXMB30.partners.org>
Message-ID: <602863D272B56749A70CBA315D7DC702062A626C@bmhexch.bmhvt.org>

Thank-You all for your responses.

-----Original Message-----
From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] 
Sent: Wednesday, August 17, 2011 10:07 AM
To: Anita Buchiane; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Creative Waste Solutions

They sell there own supplies.  We were just introduced to them by an
independent
salesperson. 


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita
Buchiane
Sent: Wednesday, August 17, 2011 9:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Creative Waste Solutions

Does anyone know of a distributor of Creative Waste Solutions products?


_______________________________________________________________



The information contained in, or attached to, this e-mail, may contain
confidential information and is intended solely for the use of the
individual or
entity to whom it is addressed and may be subject to legal privilege. If
you
have received this e-mail in error you should notify the sender
immediately by
reply e-mail, delete the message from your system and notify your system
manager. Please do not copy it for any purpose, or disclose its contents
to any
other person. The views or opinions presented in this e-mail are solely
those of
the author and do not necessarily represent those of the company. The
recipient
should check this e-mail and any attachments for the presence of
viruses. The
company accepts no liability for any damage caused, directly or
indirectly, by
any virus transmitted in this email.

_______________________________________________________________
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom
it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you
in error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.


_______________________________________________________________

The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email.
_______________________________________________________________

From jcox90 <@t> yahoo.com  Wed Aug 17 09:15:43 2011
From: jcox90 <@t> yahoo.com (Jill Cox)
Date: Wed Aug 17 09:15:49 2011
Subject: [Histonet] Ordering special stain kits and control slides
Message-ID: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>

Hi Histonetters,

I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!!?
?
Jill Cox, HT ASCP
From PAMarcum <@t> uams.edu  Wed Aug 17 09:18:53 2011
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Wed Aug 17 09:19:02 2011
Subject: [Histonet] Ordering special stain kits and control slides
In-Reply-To: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
References: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
Message-ID: 

Polyscientific R&D have very good kits and great technical back up.  

Pam Marcum
UAMS

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill Cox
Sent: Wednesday, August 17, 2011 9:16 AM
To: Histonet@Lists. Edu
Subject: [Histonet] Ordering special stain kits and control slides

Hi Histonetters,

I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!!?
?
Jill Cox, HT ASCP
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information.  Any unauthorized review,
use, disclosure or distribution is prohibited.  If you are not the 
intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message..


From trathborne <@t> somerset-healthcare.com  Wed Aug 17 09:21:34 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Wed Aug 17 09:21:56 2011
Subject: [Histonet] Ordering special stain kits and control slides
In-Reply-To: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
References: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F51CA3@SMCMAIL01.somerset-healthcare.com>

We use Poly Scientific www.PolyRnD.com Great support from their technical staff, timely deliveries, good quality.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill Cox
Sent: Wednesday, August 17, 2011 10:16 AM
To: Histonet@Lists. Edu
Subject: [Histonet] Ordering special stain kits and control slides

Hi Histonetters,

I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!!?
?
Jill Cox, HT ASCP
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.
From LSetlak <@t> childrensmemorial.org  Wed Aug 17 09:40:05 2011
From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa)
Date: Wed Aug 17 09:40:20 2011
Subject: [Histonet] Ordering special stain kits and control slides
In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F51CA3@SMCMAIL01.somerset-healthcare.com>
References: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
	<3AD061FE740D464FAC7BF6B5CFB7570711F51CA3@SMCMAIL01.somerset-healthcare.com>
Message-ID: <7111DB39D045004C9CF29E79C71B28BC1089C25371@CMHEXCC01MBX.childrensmemorial.org>

We also use Poly Scientific.
Lisa
Children's Memorial Hospital
Chg., IL

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, August 17, 2011 9:22 AM
To: 'Jill Cox'; Histonet@Lists. Edu
Subject: RE: [Histonet] Ordering special stain kits and control slides

We use Poly Scientific www.PolyRnD.com Great support from their technical staff, timely deliveries, good quality.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill Cox
Sent: Wednesday, August 17, 2011 10:16 AM
To: Histonet@Lists. Edu
Subject: [Histonet] Ordering special stain kits and control slides

Hi Histonetters,

I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!!?
?
Jill Cox, HT ASCP
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.

From turkekul <@t> gmail.com  Wed Aug 17 09:40:33 2011
From: turkekul <@t> gmail.com (mesruh turkekul)
Date: Wed Aug 17 09:40:38 2011
Subject: [Histonet] aqueous mounting media
Message-ID: 

Depending on the staining you can use different mounting media. If it is
only histological staining just mount the slides in 80% glycerol prepared in
water or compatible buffer for the staining (whatever mounting media you use
first test if it is compatable with your staining). If you have fluorescence
staining then you may need to use anti-fade media. Most anti-fade media
contain so called free radical (singlet oxygen species) scavenger chemicals
that ara not compatible with all fluorescentdyes. You have to always test if
the anti-fade mounting media is compatible with fluorescent dye you are
using. Alternatively you may use Mowiol 4-88 from Calbiochem. Mowoil does
not contain any free radical scavenger chemicals and prevents
photobleaching, it compatible with almost all fluorescent dyes.

Best,
Mesruh Turkekul MSc, HTL(ASCP)QIHC
turkekum@mskcc.org
From laurie.colbert <@t> huntingtonhospital.com  Wed Aug 17 09:40:44 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Wed Aug 17 09:40:52 2011
Subject: [Histonet] Ordering special stain kits and control slides
In-Reply-To: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
References: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AFCC@EXCHANGE3.huntingtonhospital.com>

I'm on the west coast and mainly use American Master Tech.  They also have many histology products and great technical support.  I also use Medical Chemical Corporation.  Their prices are very good, and I can always get personalized assistance from Andy Rocha - he has bailed me out on several occasions.  I'm not sure what control slides they carry, if any.
Laurie Colbert

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill Cox
Sent: Wednesday, August 17, 2011 7:16 AM
To: Histonet@Lists. Edu
Subject: [Histonet] Ordering special stain kits and control slides

Hi Histonetters,

I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!!?
?
Jill Cox, HT ASCP
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From algranth <@t> email.arizona.edu  Wed Aug 17 09:46:10 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Wed Aug 17 09:46:24 2011
Subject: [Histonet] Ordering special stain kits and control slides
In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F51CA3@SMCMAIL01.somerset-healthcare.com>
References: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
	<3AD061FE740D464FAC7BF6B5CFB7570711F51CA3@SMCMAIL01.somerset-healthcare.com>
Message-ID: 

Another vote for PolyScientific R&D but Newcomer is also good and they also control slides.

Andi Grantham




On Aug 17, 2011, at 7:21 AM, Rathborne, Toni wrote:

> We use Poly Scientific www.PolyRnD.com Great support from their technical staff, timely deliveries, good quality.
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill Cox
> Sent: Wednesday, August 17, 2011 10:16 AM
> To: Histonet@Lists. Edu
> Subject: [Histonet] Ordering special stain kits and control slides
> 
> Hi Histonetters,
> 
> I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! 
>  
> Jill Cox, HT ASCP
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical Center
> and are intended only for the addressee.  The information contained in this
> message is confidential and may contain privileged, confidential,
> proprietary and/or trade secret information entitled to protection and/or
> exemption from disclosure under applicable law.  Unauthorized forwarding,
> printing, copying, distribution, or use of such information is strictly
> prohibited and may be unlawful.  If you are not the addressee, please
> promptly delete this message and notify the sender of the delivery error
> by e-mail or you may call Somerset Medical Center's computer Help Desk
> at 908-685-2200, ext. 4050.
> 
> Be sure to visit Somerset Medical Center's Web site - 
> www.somersetmedicalcenter.com - for the most up-to-date news, 
> event listings, health information and more.
> 

From jqb7 <@t> cdc.gov  Wed Aug 17 09:49:08 2011
From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID))
Date: Wed Aug 17 09:49:15 2011
Subject: [Histonet] Ordering special stain kits and control slides
In-Reply-To: 
References: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
	<3AD061FE740D464FAC7BF6B5CFB7570711F51CA3@SMCMAIL01.somerset-healthcare.com>
	
Message-ID: 

I also use Poly Scientific and Newcomer with great success.  But occasionally I use American Mastertech when Newcomer and PS don't have what I need.

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth)
Sent: Wednesday, August 17, 2011 10:46 AM
Cc: Histonet@Lists. Edu
Subject: Re: [Histonet] Ordering special stain kits and control slides

Another vote for PolyScientific R&D but Newcomer is also good and they also control slides.

Andi Grantham




On Aug 17, 2011, at 7:21 AM, Rathborne, Toni wrote:

> We use Poly Scientific www.PolyRnD.com Great support from their technical staff, timely deliveries, good quality.
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu 
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill 
> Cox
> Sent: Wednesday, August 17, 2011 10:16 AM
> To: Histonet@Lists. Edu
> Subject: [Histonet] Ordering special stain kits and control slides
> 
> Hi Histonetters,
> 
> I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! 
>  
> Jill Cox, HT ASCP
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical 
> Center and are intended only for the addressee.  The information 
> contained in this message is confidential and may contain privileged, 
> confidential, proprietary and/or trade secret information entitled to 
> protection and/or exemption from disclosure under applicable law.  
> Unauthorized forwarding, printing, copying, distribution, or use of 
> such information is strictly prohibited and may be unlawful.  If you 
> are not the addressee, please promptly delete this message and notify 
> the sender of the delivery error by e-mail or you may call Somerset 
> Medical Center's computer Help Desk at 908-685-2200, ext. 4050.
> 
> Be sure to visit Somerset Medical Center's Web site - 
> www.somersetmedicalcenter.com - for the most up-to-date news, event 
> listings, health information and more.
> 


From sfonner <@t> labpath.com  Wed Aug 17 09:45:18 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Wed Aug 17 09:49:31 2011
Subject: [Histonet] Frozen Tissue Protocol
Message-ID: <000301cc5cec$48c81090$da5831b0$@com>

Hi everyone,

Yesterday I asked for help with modifying my protocols to stain HSVI, HSVII,
and Zoster.  I appreciate everyone's help.  Now I am back for more info.  I
deleted the depar. and cell conditioning steps and I got specific staining
with little background.  There was just one problem.the pathologist says
that the leukocytes are reacting with the alk. phos on all of the slides.
Does anyone know if a blocking step would help in this case?  Just a
reminder - I am using the Ventana Ultra platform with Ultraview Red
Detection.  Thanks again Histoland.  You never let me down! J

Sheila

 

From MSHERWOOD <@t> PARTNERS.ORG  Wed Aug 17 09:57:20 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Wed Aug 17 09:57:24 2011
Subject: [Histonet] Ordering special stain kits and control slides
In-Reply-To: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
References: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB594C@PHSXMB30.partners.org>

Ditto for Poly Scientific.  We are a research pathology lab and have used Poly
Scientific exclusively for years.  Their reagents are of excellent quality,
their tech support great and turnaround from ordering to delivery is timely.

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill Cox
Sent: Wednesday, August 17, 2011 10:16 AM
To: Histonet@Lists. Edu
Subject: [Histonet] Ordering special stain kits and control slides

Hi Histonetters,

I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do
know the various vendors but wanted your input on who you use and why if
possible. I also need to order HP control slides, same question.. Thanks in
advance!!?
?
Jill Cox, HT ASCP
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From sbaldwin <@t> mhhcc.org  Wed Aug 17 10:17:47 2011
From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org)
Date: Wed Aug 17 10:18:20 2011
Subject: [Histonet] VALIDATION
Message-ID: 

Thanks for all of those who replied I have enough samples and I hope I forwarded them to all wanted a sample!!

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
From gmartin <@t> marshallmedical.org  Wed Aug 17 10:47:09 2011
From: gmartin <@t> marshallmedical.org (Martin, Gary)
Date: Wed Aug 17 10:47:16 2011
Subject: [Histonet] Creative Waste solutions
Message-ID: <6ED9D4252F278841A0593D3D788AF24C0F76537D@mailsvr.MARSHMED.local>

If I'm not mistaken You can find Rex's unit through Creative Waste
Solutions (1-888-795-8300), Mercedes Medical, or American Master Tech
Scientific. 

 

From sfonner <@t> labpath.com  Wed Aug 17 12:12:24 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Wed Aug 17 12:16:39 2011
Subject: [Histonet] Frozen Tissue Protocol
In-Reply-To: <1313594026.91954.YahooMailRC@web1111.biz.mail.sk1.yahoo.com>
References: <000301cc5cec$48c81090$da5831b0$@com>
	<1313594026.91954.YahooMailRC@web1111.biz.mail.sk1.yahoo.com>
Message-ID: <000001cc5d00$d574da50$805e8ef0$@com>

Paula,

 

Where would I insert this step in my protocol if I am staining on the
Ventana Ultra?  Can I do it by hand after the slides are finished?  Thanks
for your help.

 

Sheila

 

 

From: Paula Pierce [mailto:contact@excaliburpathology.com] 
Sent: Wednesday, August 17, 2011 11:14 AM
To: Sheila Fonner
Subject: Re: [Histonet] Frozen Tissue Protocol

 

You are seeing endogenous phosphatase, not endogenous peroxidase. Use 10%
acetic acid for 1-2 minutes.
 

Paula K. Pierce, HTL(ASCP)HT

President

Excalibur Pathology, Inc.

631 N Broadway

Moore, OK 73160

405-759-3953 Lab

405-759-7513 Fax

www.excaliburpathology.com  

 

 

  _____  

From: Sheila Fonner 
To: histonet@lists.utsouthwestern.edu
Sent: Wed, August 17, 2011 9:45:18 AM
Subject: [Histonet] Frozen Tissue Protocol

Hi everyone,

Yesterday I asked for help with modifying my protocols to stain HSVI, HSVII,
and Zoster.  I appreciate everyone's help.  Now I am back for more info.  I
deleted the depar. and cell conditioning steps and I got specific staining
with little background.  There was just one problem.the pathologist says
that the leukocytes are reacting with the alk. phos on all of the slides.
Does anyone know if a blocking step would help in this case?  Just a
reminder - I am using the Ventana Ultra platform with Ultraview Red
Detection.  Thanks again Histoland.  You never let me down! J

Sheila



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From tnguyen <@t> kaiserassociates.com  Wed Aug 17 12:32:45 2011
From: tnguyen <@t> kaiserassociates.com (Tieuvi Nguyen)
Date: Wed Aug 17 12:33:12 2011
Subject: [Histonet] Opinions on Digital Pathology
Message-ID: <61C52EFB4F0AF8498D9ECADA6796B46618789583@VA3DIAXVS3D1.RED001.local>


Hello,

I am interested in getting in touch with some pathology professionals to get their opinions on the current and future uses of digital pathology.  Those interested please email me at: tnguyen@kaiserassociates.com with your contact information.  The conversation will last about 15 minutes and will cover current developments and current opinions on key attributes of the systems.

Thanks!

Tieuvi Nguyen  /  Kaiser Associates, Inc.
M +1 202 506 0775  /  tnguyen@kaiserassociates.com
1201 Connecticut Ave NW, 12th Floor  /  Washington, DC  20036  USA
www.kaiserassociates.com
From liz <@t> premierlab.com  Wed Aug 17 12:39:54 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Wed Aug 17 12:40:50 2011
Subject: [Histonet] RE: Opinions on Digital Pathology
In-Reply-To: <61C52EFB4F0AF8498D9ECADA6796B46618789583@VA3DIAXVS3D1.RED001.local>
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA07CD@SBS2K8.premierlab.local>

There is lots of information regarding digital pathology on the DPA website - Digital Pathology Association - http://digitalpathologyassociation.org/  and there are groups in Linked In too. http://www.linkedin.com/groups?gid=163106&trk=myg_ugrp_ovr

http://www.linkedin.com/groups?gid=3362959&trk=myg_ugrp_ovr

You can contact me if you want to discuss Digital Pathology

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tieuvi Nguyen
Sent: Wednesday, August 17, 2011 11:33 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Opinions on Digital Pathology


Hello,

I am interested in getting in touch with some pathology professionals to get their opinions on the current and future uses of digital pathology.  Those interested please email me at: tnguyen@kaiserassociates.com with your contact information.  The conversation will last about 15 minutes and will cover current developments and current opinions on key attributes of the systems.

Thanks!

Tieuvi Nguyen  /  Kaiser Associates, Inc.
M +1 202 506 0775  /  tnguyen@kaiserassociates.com
1201 Connecticut Ave NW, 12th Floor  /  Washington, DC  20036  USA
www.kaiserassociates.com

From LSetlak <@t> childrensmemorial.org  Wed Aug 17 13:19:24 2011
From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa)
Date: Wed Aug 17 13:19:33 2011
Subject: [Histonet] pathology tracking systems
Message-ID: <7111DB39D045004C9CF29E79C71B28BC1089C25379@CMHEXCC01MBX.childrensmemorial.org>



Hi,
I was wondering if anyone is currently using or has had any experience with tracking systems for histology/gross room/pathology (such as Vantage from Ventana, or Lab Lion) and what your thoughts are on them?

Thanks,
Lisa V.
Histology Manager
Children's Memorial Hospital
Chg., IL
773-868-8949
From rsrichmond <@t> gmail.com  Wed Aug 17 13:41:29 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Wed Aug 17 13:41:33 2011
Subject: [Histonet] Re: Details about Masson Trichrome Stain !
Message-ID: 

Wang Lian MD, Medical School of Nanjing University in China, asks
whether Biebrich Scarlet is Acid Red 66 or Acid Red 73.

According to R.D. Lillie (both Histologic Technic 3rd ed. and Conn's
Biological Stains 9th ed) states that the 2nd edition of the Colour
Index (a rare book I have never seen a copy of) refers to Biebrich
scarlet as Acid Red 66.

Bob Richmond
Samurai Pathologist
Knoxville, Tennessee

From NMargaryan <@t> childrensmemorial.org  Wed Aug 17 13:51:23 2011
From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira)
Date: Wed Aug 17 13:52:46 2011
Subject: [Histonet] melanin counterstaining
Message-ID: 

Good morning,

I have heavily pigmented melanocytic cells and trying to differentiate between melanin pigment and chromogen (DAB) by using Azure B.

After IHC with DAB I incubate slides for 30-45 min in the working solution of:

 *   4ml of a 25mg/ml aqueous solution of Azure B
 *   3.4ml of 0.1M acetic acid
 *   600?l of 0.1M sodium acetate
 *   27ml of DI water
 *   5ml acetone

Then water wash and hematoxylin counter-stain. By the end all melanin supposed to turn blue-green, but I still see some remained brown melanin.

My question is: I would like to increase green color, so the concentration of what reagent I have to increase?

Thanks in advance,
Naira

From solsen <@t> MPLNet.com  Wed Aug 17 13:56:51 2011
From: solsen <@t> MPLNet.com (Steve Olsen)
Date: Wed Aug 17 13:56:55 2011
Subject: [Histonet] RE: melanin counterstaining
In-Reply-To: 
References: 
Message-ID: <908827E7E3D5094BA8495A3D40ADEFE0C797F1FDA1@MAIL01.mplnet.com>

You can try a simple Schmorl's stain - which stains melanin.  You can find it in a Sheehan/Shrapchek


Steven K. Olsen
Chief Operating Officer, Director of Anatomic Pathology
Molecular Pathology Laboratory Network, Inc.
250 E. Broadway, Maryville, TN 37804

P? 865.380.9746 ext.187
C ?865.978.0321
F? 865.380.9191

http://www.MPLNET.com/
http://www.Geneuity.com/

Confidentiality Notice The information in this communication is confidential and intended for the exclusive use of the designated addressee. If you are not the addressee, any disclosure, reproduction or distribution of this communication and/or any attachments is strictly prohibited. If you are a covered entity and entitled to the information please notify us if there are exposures to the information contained in this communication as required by HIPAA and HITEC. If you received this communication in error, please notify the sender immediately.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira
Sent: Wednesday, August 17, 2011 2:51 PM
To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu
Subject: [Histonet] melanin counterstaining

Good morning,

I have heavily pigmented melanocytic cells and trying to differentiate between melanin pigment and chromogen (DAB) by using Azure B.

After IHC with DAB I incubate slides for 30-45 min in the working solution of:

 *   4ml of a 25mg/ml aqueous solution of Azure B
 *   3.4ml of 0.1M acetic acid
 *   600?l of 0.1M sodium acetate
 *   27ml of DI water
 *   5ml acetone

Then water wash and hematoxylin counter-stain. By the end all melanin supposed to turn blue-green, but I still see some remained brown melanin.

My question is: I would like to increase green color, so the concentration of what reagent I have to increase?

Thanks in advance,
Naira

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Diane.Craft <@t> amcny.org  Wed Aug 17 14:46:43 2011
From: Diane.Craft <@t> amcny.org (Diane.Craft@amcny.org)
Date: Wed Aug 17 14:40:01 2011
Subject: [Histonet] RE: melanin counterstaining
In-Reply-To: <908827E7E3D5094BA8495A3D40ADEFE0C797F1FDA1@MAIL01.mplnet.com>
References: 
	<908827E7E3D5094BA8495A3D40ADEFE0C797F1FDA1@MAIL01.mplnet.com>
Message-ID: 

I am not sure what others do, but I do not run my Azure B slides through 
alcohol because I find the alcohol diminishes the intensity of the green. 
I air dry, clear, then coverslip.

Diane Craft
Pathology Department
Animal Medical Center
510 East 62nd St
New York NY 10065-8314
212-329-8675 (phone)
212-759-5878 (fax)



Steve Olsen  
Sent by: histonet-bounces@lists.utsouthwestern.edu
08/17/2011 02:56 PM

To
"Margaryan, Naira" , 
"histonet@lists.utsouthwestern.edu" , 
"histonet-request@lists.utsouthwestern.edu" 

cc

Subject
[Histonet] RE: melanin counterstaining





You can try a simple Schmorl's stain - which stains melanin.  You can find 
it in a Sheehan/Shrapchek


Steven K. Olsen
Chief Operating Officer, Director of Anatomic Pathology
Molecular Pathology Laboratory Network, Inc.
250 E. Broadway, Maryville, TN 37804

P  865.380.9746 ext.187
C  865.978.0321
F  865.380.9191

http://www.MPLNET.com/
http://www.Geneuity.com/

Confidentiality Notice The information in this communication is 
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [
mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, 
Naira
Sent: Wednesday, August 17, 2011 2:51 PM
To: histonet@lists.utsouthwestern.edu; 
histonet-request@lists.utsouthwestern.edu
Subject: [Histonet] melanin counterstaining

Good morning,

I have heavily pigmented melanocytic cells and trying to differentiate 
between melanin pigment and chromogen (DAB) by using Azure B.

After IHC with DAB I incubate slides for 30-45 min in the working solution 
of:

*   4ml of a 25mg/ml aqueous solution of Azure B
*   3.4ml of 0.1M acetic acid
*   600?l of 0.1M sodium acetate
*   27ml of DI water
*   5ml acetone

Then water wash and hematoxylin counter-stain. By the end all melanin 
supposed to turn blue-green, but I still see some remained brown melanin.

My question is: I would like to increase green color, so the concentration 
of what reagent I have to increase?

Thanks in advance,
Naira

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From katelin09htl <@t> gmail.com  Wed Aug 17 15:08:10 2011
From: katelin09htl <@t> gmail.com (Katelin Lester)
Date: Wed Aug 17 15:08:14 2011
Subject: [Histonet] Ordering special stain kits and control slides
In-Reply-To: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
References: <1313590543.58940.YahooMailNeo@web161603.mail.bf1.yahoo.com>
Message-ID: 

I also had great results with American Mastertech's stain kits and agree
that their customer service is great. I've ordered HP control slides from
Mercedes Medical and Newcomer Supply and have had good results with both of
those as well.
-- 
Katelin Lester, HTL
Gastroenterology Specialists of Oregon, P.C.
Pathology Laboratory
(971) 224-2408



On Wed, Aug 17, 2011 at 7:15 AM, Jill Cox  wrote:

> Hi Histonetters,
>
> I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa.
> I do know the various vendors but wanted your input on who you use and why
> if possible. I also need to order HP control slides, same question.. Thanks
> in advance!!
>
> Jill Cox, HT ASCP
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From H.J.G.vandeKant <@t> uu.nl  Thu Aug 18 00:45:26 2011
From: H.J.G.vandeKant <@t> uu.nl (Kant, H.J.G. van de (Henk))
Date: Thu Aug 18 00:45:38 2011
Subject: [Histonet] T-bet antibody
Message-ID: <8A014D954ADDDD44AC39749D8EB677C90557CA@ICTSC-W-S204.soliscom.uu.nl>

Dear Colleagues,

Has somebody results with an anti mouse T-bet antibody, who is working fine on paraffin sections and formaline fixed.


Best Regards,



Henk van de Kant



From rsrichmond <@t> gmail.com  Thu Aug 18 05:25:35 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Thu Aug 18 05:25:39 2011
Subject: [Histonet] polarizers for microscopes
Message-ID: 

A microscope repairman and used microscope dealer, one I've worked
with for many years, trolls eBay for microscopes and related gear to
resell. He notes that polarizers with first-order plates ("gout
sliders") are out of manufacture and are very hard to get. It's
appalling how few pathologists I work with use polarization ("jiss
break a pair of sun glasses"). I remember my father (also a
pathologist) retrofitting his 1923 brass-tube Leitz monocular with a
polarizer in 1950. - If you've got one of these things, take care of
it - and they are fragile.

Bob Richmond
Samurai Pathologist
Knoxville TN

From Melissa.Zummak <@t> franciscanalliance.org  Thu Aug 18 07:54:44 2011
From: Melissa.Zummak <@t> franciscanalliance.org (Zummak Melissa)
Date: Thu Aug 18 07:54:50 2011
Subject: [Histonet] Benchmarks for grossing assistants/PA's
Message-ID: <30054652.1007557.1313672086188.JavaMail.root@oailxiron1.ssfhs.org>

Does anyone know where or if benchmarks exist for how many specimens someone can gross per hour or day?

Melissa Zummak



  ________________________________
The information contained in this e-mail and any accompanying documents is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited. If you are not the intended recipient(s), please contact the sender by e-mail and destroy all copies of the original message. Thank you.
From H.J.G.vandeKant <@t> uu.nl  Thu Aug 18 08:04:28 2011
From: H.J.G.vandeKant <@t> uu.nl (Kant, H.J.G. van de (Henk))
Date: Thu Aug 18 08:04:39 2011
Subject: [Histonet] Apoptosis staining on mouse testis
Message-ID: <8A014D954ADDDD44AC39749D8EB677C9055812@ICTSC-W-S204.soliscom.uu.nl>

Here a protocol for Bouin fixed tissue and it is working on testis.

TUNEL analysis on Bouin fixed, paraffin embedded tissue


  *   Incubation box in stove (37?C) with wet tissues.
  *   Dewax. All steps 3 min 3x Xylene, 2x Ehanol 100%, 1x Ethanol 96%, 1x Ethanol 70% and 1x MilliQ water.
  *   Microwave (700 Watt and at 98?C) 5 min in 10 mM Citrate buffer (pH 6.0).
  *   Put the slides directly in PBS on the shaker for 5 min.
  *   Incubate sections in 3% H202 in MilliQ water 5 min.
  *   Wash in PBS 3x5 min.
  *   Apply reaction mix:

Final concentration

1 section (30 ml)

1 section (40ml)

Amersham  buffer 1x

6ml

8 ml

Transferase 0.3U/ml

9U (0.75 ml)

1ml

dUTP 6.66 mM

0.2 ml

0.27 ml

MilliQ water (sterile)

23.05 ml

30.73 ml


For small sections apply 30 ml per section, 40 ml for bigger sections
NB! For negative control apply reaction mix without transferase


  *   Incubate 1h at 37?C in damp atmosphere

  *   End of reaction: Apply to each section the following buffer:
300mM NaCl, 30mM NaCitrate in MilliQ water and incubate for 15 min at RT

  *   Wash in PBS 3x5 min
  *   Blockage of non-specific sites with 2% BSA in PBS for 10 min at RT
  *   Apply ExtrAvidine 1:20 in 1%BSA PBS (30 or 40 ml/section) to sections and incubate 30 min at 37?C in damp atmosphere

  *   Wash in PBS 3x5 min

  *   Incubate in DAB solution (125 mgr DAB / 250 ml PBS + 85ml H2O2  37%) Incubate for 1 and 2 min. Use gloves and work in a fume cupboard.
  *   Wash in MilliQ water 5 min.
  *   Counterstain with Mayer for 30 sec.
  *   Dehydrate and mount with Pertex .
From tammy <@t> surgicalpathlabs.com  Thu Aug 18 08:23:09 2011
From: tammy <@t> surgicalpathlabs.com (Tammy de Leon)
Date: Thu Aug 18 08:27:05 2011
Subject: [Histonet] job openings
Message-ID: 

 

 

If you're looking for a friendly environment, a new state-of-the-art
facility and an employer of choice, SPL is the place for you!! We are
currently looking for qualified Histotechnicians/Histotechnologists!

 

Histo candidates should be an HTL or HT (ASCP) or equivalent.

Primary responsibilities include on-site frozen sections including mobile
laboratory units.

 

SPL is CAP accredited, offers competitive pay, and a comprehensive benefits
package.  SPL pays 100% of employee premiums for  Medical, Dental, LTD and
Life!  We also offer a Retirement Plan & Supplemental Insurance.

 

Please forward resume to

Tammy de Leon

tammy@surgicalpathlabs.com

Surgical Pathology Laboratory

800-304-1066

8455 66th Street N

Pinellas Park, Florida 33781

www.surgicalpathlabs.com

 

From thisisann <@t> aol.com  Thu Aug 18 09:19:34 2011
From: thisisann <@t> aol.com (Ann Angelo)
Date: Thu Aug 18 09:19:47 2011
Subject: [Histonet] Embedding Credentials
Message-ID: <8CE2BC8FD45804D-1CB8-D0F7@Webmail-m105.sysops.aol.com>

Does anyone know if CAP or NYS require an embedder to have specific credentials?  If so, what are they?  Thank you, Ann


From rjbuesa <@t> yahoo.com  Thu Aug 18 09:49:56 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Aug 18 09:50:04 2011
Subject: [Histonet] Benchmarks for grossing assistants/PA's
In-Reply-To: <30054652.1007557.1313672086188.JavaMail.root@oailxiron1.ssfhs.org>
Message-ID: <1313678996.32877.YahooMailClassic@web65702.mail.ac4.yahoo.com>

I have attached an article on the subject to your e-mail address
Ren? J.

--- On Thu, 8/18/11, Zummak Melissa  wrote:


From: Zummak Melissa 
Subject: [Histonet] Benchmarks for grossing assistants/PA's
To: "'histonet@lists.utsouthwestern.edu'" 
Date: Thursday, August 18, 2011, 8:54 AM


Does anyone know where or if benchmarks exist for how many specimens someone can gross per hour or day?

Melissa Zummak



? ________________________________
The information contained in this e-mail and any accompanying documents is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited. If you are not the intended recipient(s), please contact the sender by e-mail and destroy all copies of the original message. Thank you.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From coughlin <@t> musc.edu  Thu Aug 18 10:10:11 2011
From: coughlin <@t> musc.edu (Coughlin, Beth)
Date: Thu Aug 18 10:22:32 2011
Subject: [Histonet] Araldite
Message-ID: <1048F6FE-5817-4B33-BF31-81E3576790B8@musc.edu>

Anyone have advice for how to get sections from epon blocks that are very soft??
I used a rapid embedding protocol for making epon blocks that I found online.
Unfortunately, I misunderstood the instruction that I was to use araldite alone for the embedding media.
I used the kit that came with the araldite (ddsa and dmp 30).
I didn't actually use the steps for polymerizing recommended below. I just used the early steps for speeding up the processing. I just put my samples in at 60 degrees overnight, as usual.



WASH...............PBS..................2X5 MIN.

1%OsO4 in PBS...........................20 MIN RTEMP.

WASH...............PBS..................2X5 MIN

*70% ETOH...............................3 MIN

95% ETOH................................3 MIN

100% ETOH...............................2X5 MIN

PROPYLENE OXIDE.........................2X5 MIN

1:1 P.O.:ARALDITE 502...................30 MIN (REMOVE Caps)

1:3 " " ...................20 MIN

PURE ARALDITE 502.......................10 MIN

EMBED

CURE IN 75C OVEN FOR 50 MIN. PLACE IN 95C OVEN FOR 50 MIN.

PLACE IN FREEZER AND LET COOL 10 MIN.

From alisha <@t> ka-recruiting.com  Thu Aug 18 10:32:34 2011
From: alisha <@t> ka-recruiting.com (Alisha Dynan)
Date: Thu Aug 18 10:31:51 2011
Subject: [Histonet] Exciting Histotech Job in Arizona
Message-ID: <1953872259.1313681554881.JavaMail.cfservice@SL4APP4>


Dear Histonet Members,

I hope you are doing well. I am a healthcare recruiter and I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. 













 

Histotech in Arizona!


I am currently working with a client in rural Arizona.  This 250 bed non profit medical center has been ranked one of the top 100 hospitals for community value. My client is currently looking for qualified Histotechs with to fill 2 openings. My client is looking for candidates who are HT(ASCP) or HTL(ASCP) certified and have at least 1 year of histology experience. This hospital offers a very competitive base salary/hourly rate, shift differentials, an outstanding benefits and retirement package, relocation assistance, and a retention bonus. If you are interested in learning more about this state of the art facility in Arizona, email me at alisha@ka-recruiting.com.

 







If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future.  I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.  

 


To view some additional opportunities please visit our website at www.ka-recruiting.com.  





















Sincerely,

 

Alisha Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

alisha@ka-recruiting.com

www.ka-recruiting.com

 




 


From pathlocums <@t> gmail.com  Thu Aug 18 10:34:03 2011
From: pathlocums <@t> gmail.com (Davide Costanzo)
Date: Thu Aug 18 10:34:06 2011
Subject: [Histonet] Thermo STP420 and Excelsior
Message-ID: 

Dear Colleagues,

We are in the process of opening a new lab and are looking for a rapid
processor, preferably non-microwave, and a standard processor for backup. At
present I have been looking into the Thermo STP420 with an Excelsior backup.
My concerns are that I have heard some very negative feedback on the
Excelsior.

Can anyone tell me their experiences, good or bad, with both the STP420 and
the Excelsior? If you are using, or have used either one I would greatly
appreciate your thoughts. In addition, we are considering alternatives that
include the Peloris by Leica. It is somewhat more costly at current time and
thus the reason for Thermo STP420 to be in the lead on this one.

Should we use the Excelsior as backup givin its reagent savings, or go with
the VIP 6?

Thank you for sharing your thoughts.


David Costanzo
Project Manager
BluFrog Path Lab
From Edina.Paal <@t> va.gov  Thu Aug 18 12:34:17 2011
From: Edina.Paal <@t> va.gov (Paal, Edina E.)
Date: Thu Aug 18 12:34:37 2011
Subject: [Histonet] Cellient
Message-ID: <709792116CA04F4BABB197F941EB57DA013CD26FF8D8@R04BYNMSGD1.r04.med.va.gov>

Does anybody out there have experience with Cellient? We got some samples run by Hologic and our histotechs had a hard time cutting the cell blocks due to the rigid paraffin. Apparently this is the only type of paraffin validated for the instrument. I find it hard to believe that changing the paraffin is a major obstacle... would appreciate your opinion.





Edina Paal, M.D.

Pathologist, Cytopathologist, Director of Blood Bank

Pathology and Laboratory Medicine Service

VA Medical Center, Washington DC

50 Irving St, N.W., Rm GB205

Washington, DC 20422



Tel: (202) 518-4619

Fax: (202) 745-8284



From jengirl1014 <@t> yahoo.com  Thu Aug 18 12:34:56 2011
From: jengirl1014 <@t> yahoo.com (Jennifer Sipes)
Date: Thu Aug 18 12:35:01 2011
Subject: [Histonet] Re: Histonet Digest, Vol 93, Issue 24
Message-ID: <1313688896.88203.YahooMailNeo@web125401.mail.ne1.yahoo.com>

Hi everyone!
?
First let me thank EVERYONE for the huge response on the H&E stain on frozen tissue!!! My slides turned out great!
?
Now, I've got a question that I'm thinking will stump you as much as it's stumped a co-worker and I!? She is doing a study on the mucus membrane in the intestinal tract(cecum and colon mainly) of the mouse.? She tried flushing the fecal matter out of the colon and cecum but lost the membrane.? She decided to leave the fecal matter in, however now when I section it, it tears up my blade and gives sections that are not all that great.? I was wondering if anyone knows how to acheive great sections while keeping the membrane?? 
?
Thank you so much in advance for any and all help you can give us!
?
Regards,
?
Jen
?
Jennifer K. Sipes, ALAT 
Sr. Laboratory Technician 
Johns Hopkins University 
Ross 933 
720 Rutland Avenue 
Baltimore, MD? 21205 
phone:???? 410-614-0131 
fax:???????? 410-955-9677 
cell:???????? 443-631-6361 
e-mail:? jsipes1@jhmi.edu 
From oneilb <@t> wvuhealthcare.com  Thu Aug 18 12:42:53 2011
From: oneilb <@t> wvuhealthcare.com (Oneil, Beth Ann)
Date: Thu Aug 18 12:41:45 2011
Subject: [Histonet] IHC control material
Message-ID: <052C41417E0EF64D8E201FE822D36CA90A1C5A36@NT-EXCHANGE.wvuh.wvuhs.com>

   We would like to re-organize our system for maintaining blocks and slides for our IHC section.  Currently, we have a cache of blocks, each labelled with the different antibody it is used for, and filed in a drawer.  When we begin to run low on tissue, we have to scurry around and find a good specimen to use and lately it's been a patient block that we'll have to cut into. We don't like doing that because what if our docs order additional testing.   
  With our control slides, we'll cut around 10 at a time (50 if tonsil) and label with the antibody name and Julian date so that we know how old the slide is.  This seems to work pretty well.  
  I guess my question is, how do others organize their control tissues for IHC.
 
Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor
West Virginia University Hospitals
304-293-6014


-----------------------------------------
Confidentiality Notice: This e-mail message, including any
attachments, is for the sole use of the intended recipient(s) and
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 If you are not the intended recipient, please contact the sender
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From Barbara.Crill <@t> LPNT.net  Thu Aug 18 12:47:11 2011
From: Barbara.Crill <@t> LPNT.net (Barbara.Crill@LPNT.net)
Date: Thu Aug 18 12:47:20 2011
Subject: [Histonet] lab coats
Message-ID: <7DA79EBDBD92BF408EF392413737878D3948063B5D@NADCWPMSGCMS01.hca.corpad.net>

We are looking for new lab coats.
We are trying to find lab coats that are not HOT but yet fluid resistant.

We tried DenLine but our techs are not really happy with the blue coats they sell.


Any suggestions?


ANTOINETTE CRILL, MBA,CT(ASCP)
TEAM LEADER ANATOMIC PATHOLOGY
From trathborne <@t> somerset-healthcare.com  Thu Aug 18 12:47:55 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Thu Aug 18 12:48:06 2011
Subject: [Histonet] Re: Histonet Digest, Vol 93, Issue 24
In-Reply-To: <1313688896.88203.YahooMailNeo@web125401.mail.ne1.yahoo.com>
References: <1313688896.88203.YahooMailNeo@web125401.mail.ne1.yahoo.com>
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F52E72@SMCMAIL01.somerset-healthcare.com>

Have you thought of withholding food from the mice before euthanizing them? Or are these spontaneous deaths?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes
Sent: Thursday, August 18, 2011 1:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 93, Issue 24

Hi everyone!
?
First let me thank EVERYONE for the huge response on the H&E stain on frozen tissue!!! My slides turned out great!
?
Now, I've got a question that I'm thinking will stump you as much as it's stumped a co-worker and I!? She is doing a study on the mucus membrane in the intestinal tract(cecum and colon mainly) of the mouse.? She tried flushing the fecal matter out of the colon and cecum but lost the membrane.? She decided to leave the fecal matter in, however now when I section it, it tears up my blade and gives sections that are not all that great.? I was wondering if anyone knows how to acheive great sections while keeping the membrane?? 
?
Thank you so much in advance for any and all help you can give us!
?
Regards,
?
Jen
?
Jennifer K. Sipes, ALAT 
Sr. Laboratory Technician 
Johns Hopkins University 
Ross 933 
720 Rutland Avenue 
Baltimore, MD? 21205 
phone:???? 410-614-0131 
fax:???????? 410-955-9677 
cell:???????? 443-631-6361 
e-mail:? jsipes1@jhmi.edu 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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Be sure to visit Somerset Medical Center's Web site - 
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From Reuel.Cornelia <@t> tsrh.org  Thu Aug 18 13:37:35 2011
From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia)
Date: Thu Aug 18 13:37:58 2011
Subject: [Histonet] MMA for EM
Message-ID: <4E4D159F.077E.00C5.1@tsrh.org>

Can MMA embedded tissue be use for Electron Microscopy. If not,can anyone have a procedure how to prepare tissue that are embedded in MMA for EM. Is this the same procedure done on a paraffin embedded tissue. Thank you for your help.
 
Reuel
From Reuel.Cornelia <@t> tsrh.org  Thu Aug 18 14:06:15 2011
From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia)
Date: Thu Aug 18 14:06:28 2011
Subject: [Histonet] MMA
Message-ID: <4E4D1C57.077E.00C5.1@tsrh.org>

Can undecalcified bone MMA embedded tissue be use for Electron Microscopy.The tissue was fixed in formalin dehydrated in grades of alcohol, clear in xylene, infiltrated and embedded in MMA (MMA, dibutyl phthalate,perakdox).  If not,can anyone have a procedure how to prepare tissue that are embedded in MMA for EM. Is this the same procedure done on a paraffin embedded tissue where you melt the paraffin with xylene then hydrate, wash in distilled water then transfer in osmium ,wash in water, dehydrate, PO,resin. 
 
Thank you for your help.
 
Reuel
From algranth <@t> email.arizona.edu  Thu Aug 18 14:13:09 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Thu Aug 18 14:13:23 2011
Subject: [Histonet] Re: Histonet Digest, Vol 93, Issue 24
In-Reply-To: <1313688896.88203.YahooMailNeo@web125401.mail.ne1.yahoo.com>
References: <1313688896.88203.YahooMailNeo@web125401.mail.ne1.yahoo.com>
Message-ID: <6D66F841-D164-4529-947B-45A3CAC7BAEE@email.arizona.edu>

Has she tried opening the colons and allowing them to fix and then gently flushing the fecal matter out before processing?

Andi

On Aug 18, 2011, at 10:34 AM, Jennifer Sipes wrote:

> Hi everyone!
>  
> First let me thank EVERYONE for the huge response on the H&E stain on frozen tissue!!! My slides turned out great!
>  
> Now, I've got a question that I'm thinking will stump you as much as it's stumped a co-worker and I!  She is doing a study on the mucus membrane in the intestinal tract(cecum and colon mainly) of the mouse.  She tried flushing the fecal matter out of the colon and cecum but lost the membrane.  She decided to leave the fecal matter in, however now when I section it, it tears up my blade and gives sections that are not all that great.  I was wondering if anyone knows how to acheive great sections while keeping the membrane?  
>  
> Thank you so much in advance for any and all help you can give us!
>  
> Regards,
>  
> Jen
>  
> Jennifer K. Sipes, ALAT 
> Sr. Laboratory Technician 
> Johns Hopkins University 
> Ross 933 
> 720 Rutland Avenue 
> Baltimore, MD  21205 
> phone:     410-614-0131 
> fax:         410-955-9677 
> cell:         443-631-6361 
> e-mail:  jsipes1@jhmi.edu 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From NMargaryan <@t> childrensmemorial.org  Thu Aug 18 14:31:05 2011
From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira)
Date: Thu Aug 18 14:33:04 2011
Subject: [Histonet] H&E on  cells, protocol
Message-ID: 


Hi Histonetters,

I have to do H&E staining on cells that were growing up on coverslips. Can anyone send me a good protocol to fix these cells on same coverslip and H&E staining, please?

Thanks in advance,
Naira

From candice_camille <@t> yahoo.com  Thu Aug 18 14:38:12 2011
From: candice_camille <@t> yahoo.com (Candice Smoots)
Date: Thu Aug 18 14:38:14 2011
Subject: [Histonet] Silver Gold intensification
Message-ID: <1313696292.51315.YahooMailNeo@web125409.mail.ne1.yahoo.com>

Hey All
?
Does anyone have a silver gold intensification procedure that you could send me. I am doing this for the first time and I am not sure of what the tissue is supposed to look like. 
?
If at all possible, could u send the protocol as well as guidelines as to what this tissue is supposed to look like. If not, The protocol should suffice.
?
Thank You all soooooooooooooooo much!

I remain yours truely, 

Candice Camille 
From ratliffjack <@t> hotmail.com  Thu Aug 18 14:44:12 2011
From: ratliffjack <@t> hotmail.com (Jack Ratliff)
Date: Thu Aug 18 14:44:17 2011
Subject: [Histonet] MMA
In-Reply-To: <4E4D1C57.077E.00C5.1@tsrh.org>
References: <4E4D1C57.077E.00C5.1@tsrh.org>
Message-ID: 


Reuel,
 
I would say that you could at least try to use for EM, especially since you already seem to have a polymerized resin block. The biggest issue I would guess would be the overall hardness of your resin block/section so that the section would hold up during the analysis. Also, depending upon the hardness of your block, you may even be able to put your blocks in an oven at 60C for a weekend or so to harden them up even more. It theorized that even though the hardness or softness of a MMA block can be established from the beginning of polymerization (solution concentration of monomer:softner), the block continues to harden over time. I would say subjecting to heat may help to increase this however it may make it more difficult to cut thin sections. In fact, if you let your section roll during microtomy, you may need to adapt to pulling sections flat as the block continues to harden. Hope this makes some sense. Feel free to call me (317-281-1975) if you would like to talk about it over the phone.
 
Jack
 

 

> Date: Thu, 18 Aug 2011 14:06:15 -0500
> From: Reuel.Cornelia@tsrh.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] MMA
> 
> Can undecalcified bone MMA embedded tissue be use for Electron Microscopy.The tissue was fixed in formalin dehydrated in grades of alcohol, clear in xylene, infiltrated and embedded in MMA (MMA, dibutyl phthalate,perakdox). If not,can anyone have a procedure how to prepare tissue that are embedded in MMA for EM. Is this the same procedure done on a paraffin embedded tissue where you melt the paraffin with xylene then hydrate, wash in distilled water then transfer in osmium ,wash in water, dehydrate, PO,resin. 
> 
> Thank you for your help.
> 
> Reuel
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From MSHERWOOD <@t> PARTNERS.ORG  Thu Aug 18 15:14:42 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Thu Aug 18 15:14:47 2011
Subject: [Histonet] Re: Histonet Digest, Vol 93, Issue 24
In-Reply-To: <6D66F841-D164-4529-947B-45A3CAC7BAEE@email.arizona.edu>
References: <1313688896.88203.YahooMailNeo@web125401.mail.ne1.yahoo.com>
	<6D66F841-D164-4529-947B-45A3CAC7BAEE@email.arizona.edu>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB595A@PHSXMB30.partners.org>

I would like to give input on this problem.  We had a similar situation.  The
investigators couldn't flush the fecal material before fixing-destroyed the
treated area of interest.  After fixing, you couldn't flush the material out.
We ended up manually "picking out the material" before embedding (messy and not
fun!).  (P.S.  Food was withheld from the rats before euthanization).

If anyone has a suggestion, we would greatly appreciate hearing about it.

Peggy 

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea
L - (algranth)
Sent: Thursday, August 18, 2011 3:13 PM
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Histonet Digest, Vol 93, Issue 24

Has she tried opening the colons and allowing them to fix and then gently
flushing the fecal matter out before processing?

Andi

On Aug 18, 2011, at 10:34 AM, Jennifer Sipes wrote:

> Hi everyone!
>  
> First let me thank EVERYONE for the huge response on the H&E stain on frozen
tissue!!! My slides turned out great!
>  
> Now, I've got a question that I'm thinking will stump you as much as it's
stumped a co-worker and I!  She is doing a study on the mucus membrane in the
intestinal tract(cecum and colon mainly) of the mouse.  She tried flushing the
fecal matter out of the colon and cecum but lost the membrane.  She decided to
leave the fecal matter in, however now when I section it, it tears up my blade
and gives sections that are not all that great.  I was wondering if anyone knows
how to acheive great sections while keeping the membrane?  
>  
> Thank you so much in advance for any and all help you can give us!
>  
> Regards,
>  
> Jen
>  
> Jennifer K. Sipes, ALAT 
> Sr. Laboratory Technician 
> Johns Hopkins University 
> Ross 933 
> 720 Rutland Avenue 
> Baltimore, MD  21205 
> phone:     410-614-0131 
> fax:         410-955-9677 
> cell:         443-631-6361 
> e-mail:  jsipes1@jhmi.edu 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



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From tnguyen <@t> kaiserassociates.com  Thu Aug 18 15:23:33 2011
From: tnguyen <@t> kaiserassociates.com (Tieuvi Nguyen)
Date: Thu Aug 18 15:23:48 2011
Subject: [Histonet] subscription update
Message-ID: <61C52EFB4F0AF8498D9ECADA6796B4661878958E@VA3DIAXVS3D1.RED001.local>


Hi,

Can you please remove me from receiving message board comments not related to comments I have posted?

Thanks,
Tieuvi


Tieuvi Nguyen  /  Kaiser Associates, Inc.
M +1 202 506 0775  /  tnguyen@kaiserassociates.com
1201 Connecticut Ave NW, 12th Floor  /  Washington, DC  20036  USA
www.kaiserassociates.com
From dr_sadushe <@t> yahoo.com  Thu Aug 18 16:01:40 2011
From: dr_sadushe <@t> yahoo.com (Sadushe Loxha)
Date: Thu Aug 18 16:01:43 2011
Subject: [Histonet] (no subject)
Message-ID: <1313701300.19897.YahooMailMobile@web36102.mail.mud.yahoo.com>

http://superratings.info/oldtemp/frkiif.htm
From utebachmeier <@t> gmail.com  Thu Aug 18 16:19:53 2011
From: utebachmeier <@t> gmail.com (Sabrina Friedman)
Date: Thu Aug 18 16:19:58 2011
Subject: [Histonet] Opening in KC, Missourifrom Sabrina
Message-ID: 

Full time or part time opening in Kansas City, Missouri for a busy
Dermatopathology lab. Must be HT or HTL certified. Proficient in all
aspects of routine histology. Grossing, coversliping, accessioning,
microtonomy, and H&E stains. Please call Tami at 816.584.8100 for
immediate consideration.




Sabrina Friedman
Billige Fl?ge Marketing GmbH
Emanuelstr. 3,
10317 Berlin
Deutschland
Telefon: +49 (33) 5310967
Email: utebachmeier@gmail.com
Site: http://flug.airego.de - Billige Fl?ge vergleichen

From Christina.Wilson <@t> leica-microsystems.com  Thu Aug 18 17:36:57 2011
From: Christina.Wilson <@t> leica-microsystems.com (Christina.Wilson@leica-microsystems.com)
Date: Thu Aug 18 17:36:58 2011
Subject: [Histonet] AUTO:  is out of the office. (returning Fri 08/19/2011)
Message-ID: 


I am out of the office from Thu 08/18/2011 until Fri 08/19/2011.

I will have limited access to emails during this time.  If you should need
assistance, please contact Demaris Mills,
demaris.mills@leica-microsystems.com, for product management support or
Karen Niewerth, karen.niewerth@leica-microsystems.com, for customer service
support.


Note: This is an automated response to your message  "Histonet Digest, Vol
93, Issue 24" sent on 8/18/2011 12:00:00 PM.

This is the only notification you will receive while this person is away.


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From ttruscot <@t> vetmed.wsu.edu  Thu Aug 18 17:41:21 2011
From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom)
Date: Thu Aug 18 17:41:52 2011
Subject: [Histonet] Re: Histonet Digest, Vol 93, Issue 24
In-Reply-To: <6D66F841-D164-4529-947B-45A3CAC7BAEE@email.arizona.edu>
References: <1313688896.88203.YahooMailNeo@web125401.mail.ne1.yahoo.com>
	<6D66F841-D164-4529-947B-45A3CAC7BAEE@email.arizona.edu>
Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00270A5C55B5@CVM76.vetmed.wsu.edu>

Or, has you tried gently infusing the intestinal lumen with formalin from a syringe and tying or clamping both ends, then allow to fix in a jar of formalin, then trim open lengthways, then trim C-shaped cross-sections, then remove fecal material before placing in cassette. Tom T

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth)
Sent: Thursday, August 18, 2011 12:13 PM
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Histonet Digest, Vol 93, Issue 24

Has she tried opening the colons and allowing them to fix and then gently flushing the fecal matter out before processing?

Andi

On Aug 18, 2011, at 10:34 AM, Jennifer Sipes wrote:

> Hi everyone!
>  
> First let me thank EVERYONE for the huge response on the H&E stain on frozen tissue!!! My slides turned out great!
>  
> Now, I've got a question that I'm thinking will stump you as much as it's stumped a co-worker and I!  She is doing a study on the mucus membrane in the intestinal tract(cecum and colon mainly) of the mouse.  She tried flushing the fecal matter out of the colon and cecum but lost the membrane.  She decided to leave the fecal matter in, however now when I section it, it tears up my blade and gives sections that are not all that great.  I was wondering if anyone knows how to acheive great sections while keeping the membrane?  
>  
> Thank you so much in advance for any and all help you can give us!
>  
> Regards,
>  
> Jen
>  
> Jennifer K. Sipes, ALAT 
> Sr. Laboratory Technician 
> Johns Hopkins University 
> Ross 933 
> 720 Rutland Avenue 
> Baltimore, MD  21205 
> phone:     410-614-0131 
> fax:         410-955-9677 
> cell:         443-631-6361 
> e-mail:  jsipes1@jhmi.edu 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From wgray19 <@t> sc.rr.com  Thu Aug 18 19:20:39 2011
From: wgray19 <@t> sc.rr.com (Jeff and Wanda Gray)
Date: Thu Aug 18 19:21:30 2011
Subject: [Histonet] Decalcifying...Again
Message-ID: <001b01cc5e05$d32ed150$798c73f0$@rr.com>

'Netters:
I know the subject has been addressed ad nauseum on here, but my favorite
pathologist asked really nicely, so here goes: What are you using to
decalcify head and neck specimen? For how long ( a range is what we're
after)? Does immuno still work on the decalcified specimen? We get head and
neck resections almost every day, and are having trouble with turnaround
time. Often, even after three days in a commercial 10% HCl decal solution,
we still have great difficulty cutting these, with problems such as chipping
out and inability of all of our cutters to get artifact free sections. Both
the docs and we are frustrated and begging for input.
Thanks in advance,
Wanda Shotsberger Gray HTL (ASCP)
MUSC
Chas., SC
From louise.renton <@t> gmail.com  Fri Aug 19 04:17:33 2011
From: louise.renton <@t> gmail.com (Louise Renton)
Date: Fri Aug 19 04:17:39 2011
Subject: [Histonet] cadaveric bone
Message-ID: 

Hi all - a somewhat morbid question for the weekend. A student in the
anatomy dept came to me with wax embedded samlpes of demineralized
bone from dissection cadavers. The bone is very flinty and difficult
to section. Is this perhaps due to the preseravation/embalming process
that the bodies undergo? Is there something I can do to alleviate this
problem prior to processing?

I have tried  dewaxing and furher demineralization, but the problem
sems to be in the matrix itself--

BTW, using my usual protocol I ahv been able to section elephant tusk
- so I think the prob is the bone rather than what I am doing to it


best love & haev a great weekend

Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.

From talulahgosh <@t> gmail.com  Fri Aug 19 04:55:37 2011
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Fri Aug 19 04:55:40 2011
Subject: [Histonet] cadaveric bone
In-Reply-To: 
References: 
Message-ID: 

Wow, you section elephant tusk?
That is so cool.
And also, let me know what the answer is to your question, it's an
interesting one.

Emily


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Fri, Aug 19, 2011 at 5:17 AM, Louise Renton wrote:

> Hi all - a somewhat morbid question for the weekend. A student in the
> anatomy dept came to me with wax embedded samlpes of demineralized
> bone from dissection cadavers. The bone is very flinty and difficult
> to section. Is this perhaps due to the preseravation/embalming process
> that the bodies undergo? Is there something I can do to alleviate this
> problem prior to processing?
>
> I have tried  dewaxing and furher demineralization, but the problem
> sems to be in the matrix itself--
>
> BTW, using my usual protocol I ahv been able to section elephant tusk
> - so I think the prob is the bone rather than what I am doing to it
>
>
> best love & haev a great weekend
>
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
> "There are nights when the wolves are silent and only the moon howls".
> George Carlin
> No trees were killed in the sending of this message.
> However, many electrons were terribly inconvenienced.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From Melissa.Kuhnla <@t> chsli.org  Fri Aug 19 06:51:58 2011
From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa)
Date: Fri Aug 19 06:52:20 2011
Subject: [Histonet] IHC control material
In-Reply-To: <052C41417E0EF64D8E201FE822D36CA90A1C5A36@NT-EXCHANGE.wvuh.wvuhs.com>
References: <052C41417E0EF64D8E201FE822D36CA90A1C5A36@NT-EXCHANGE.wvuh.wvuhs.com>
Message-ID: 

Hi,
First let me say that a proper control system is not possible without
the help of a pathologist.  I am lucky enough to have a pathologist that
continuously is dedicated to helping me with this project. He very much
understands how important it is!! 
The pathologist is constantly looking for cases that have additional
tissue that can be processed, H&Ed, stained with various antibodies, and
then cataloged and documented as far as what it can be used for in the
future as a control.  After about 4 years of this, I have a library of a
couple hundred cases. Some are rare tumors; some are normal tonsil and
colon...etc.  I have excel spreadsheets that log case number, tissue
type, hours fixation, stain results, date, pathologist...etc.

I then have a naming and numbering system for 'working control' blocks.
In other words, our mesothelioma control block contains three or four
different tissue types. I have a log that shows all the case numbers and
that they were combined into working control blocks. Say I make five
blocks at once, they are named meso1, meso2, meso3, meso4, meso5. I then
H&E these 'working control' blocks and the pathologist signs off on
them. Now they are ready to put into use and cut.  We do pre-cut
controls. The slide is labeled with the blocks name (meso1), the date
cut, and the cutters initials.  This is so we are always using the
oldest first.

Hope this helps. Let me know if you need anything further.
Melissa

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Oneil,
Beth Ann
Sent: Thursday, August 18, 2011 1:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC control material

   We would like to re-organize our system for maintaining blocks and
slides for our IHC section.  Currently, we have a cache of blocks, each
labelled with the different antibody it is used for, and filed in a
drawer.  When we begin to run low on tissue, we have to scurry around
and find a good specimen to use and lately it's been a patient block
that we'll have to cut into. We don't like doing that because what if
our docs order additional testing.   
  With our control slides, we'll cut around 10 at a time (50 if tonsil)
and label with the antibody name and Julian date so that we know how old
the slide is.  This seems to work pretty well.  
  I guess my question is, how do others organize their control tissues
for IHC.
 
Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor
West Virginia University Hospitals
304-293-6014


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From ratliffjack <@t> hotmail.com  Fri Aug 19 06:58:32 2011
From: ratliffjack <@t> hotmail.com (Jack Ratliff)
Date: Fri Aug 19 06:58:45 2011
Subject: [Histonet] cadaveric bone
In-Reply-To: 
References: 
Message-ID: 

Louise,

My first suggestion to you is to de-wax the specimen and process into an MMA based resin containing a softener like DBP. Once polymerized you can simply take thin sections using tungsten-carbide knives. Make sure to infiltrate the specimen well with the resin solution prior to embedding. If you are unable to try this method, I would be happy to try this for you free of charge if you could send me the specimen.

Regarding further specimens of this type, again just process, infiltrate and embed in the MMA based resin without any prior wax or decalcification. If interrsted, you could also send me two of these fresh specimens that have not been decalcified or processed into wax and I could cut them for you as well for a proof of concept. I would process, infiltrate and embed one specimen in Acrylosin (MMA resin from Dorn & Hart Microedge) and with the other I have another method I try where I could cut the tissue without additional processing or embedding (similar I guess to cryotomy without the freezing process) at a minimum of 10 microns using a laser microtome! Yes, I said a laser microtome! :)

What do you say? Contact me back if you are interested and we can discuss the shipping details.

Best Regards,


Jack


Jack L Ratliff
Senior Histologist, BioMimetic Therapeutics
Chairman, Hard Tissue Committee - National Society for Histotechnology






On Aug 19, 2011, at 4:17 AM, Louise Renton wrote:

> Hi all - a somewhat morbid question for the weekend. A student in the
> anatomy dept came to me with wax embedded samlpes of demineralized
> bone from dissection cadavers. The bone is very flinty and difficult
> to section. Is this perhaps due to the preseravation/embalming process
> that the bodies undergo? Is there something I can do to alleviate this
> problem prior to processing?
> 
> I have tried  dewaxing and furher demineralization, but the problem
> sems to be in the matrix itself--
> 
> BTW, using my usual protocol I ahv been able to section elephant tusk
> - so I think the prob is the bone rather than what I am doing to it
> 
> 
> best love & haev a great weekend
> 
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
> "There are nights when the wolves are silent and only the moon howls".
> George Carlin
> No trees were killed in the sending of this message.
> However, many electrons were terribly inconvenienced.
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From Vickroy.Jim <@t> mhsil.com  Fri Aug 19 06:58:40 2011
From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim)
Date: Fri Aug 19 06:58:50 2011
Subject: [Histonet] True-Bond 380 slides
Message-ID: <24A4826E8EF0964D86BC5317306F58A55FC28704BE@mmc-mail.ad.mhsil.com>


Recently we switched to a different slide, cost savings.   The slide is called True-Bond 380.  This morning we noticed that the slides had an eosin tint in the background.   We are going to play with it to get rid of it but has anybody else experienced this problem and how did they solve it?  We are going to extend the 95% after hematoxylin but just wanted other ideas.

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
From SEsparza <@t> seton.org  Fri Aug 19 07:48:21 2011
From: SEsparza <@t> seton.org (Esparza, Sandra)
Date: Fri Aug 19 07:48:25 2011
Subject: [Histonet] True-Bond 380 slides
In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55FC28704BE@mmc-mail.ad.mhsil.com>
References: <24A4826E8EF0964D86BC5317306F58A55FC28704BE@mmc-mail.ad.mhsil.com>
Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB041DFE34@AUSEX2VS1.seton.org>

We use the True-Bond 200 from StatLab for our IHC's, special stain, bone
marrows, muscle enzymes, and any bloody tissue.  We have not had a
problem with tint in the background.  We use phloxine eosin.  You might
want to try the 200 slides.

Sandra
Sandra Esparza HT(ASCP)QIHC
Lead Technologist
Dell Children's Medical Center of Central Texas
512-324-0000  x87061
sesparza@seton.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Friday, August 19, 2011 6:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] True-Bond 380 slides


Recently we switched to a different slide, cost savings.   The slide is
called True-Bond 380.  This morning we noticed that the slides had an
eosin tint in the background.   We are going to play with it to get rid
of it but has anybody else experienced this problem and how did they
solve it?  We are going to extend the 95% after hematoxylin but just
wanted other ideas.

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


________________________________
This message (including any attachments) contains confidential
information intended for a specific individual and purpose, and is
protected by law. If you are not the intended recipient, you should
delete this message. Any disclosure, copying, or distribution of this
message, or the taking of any action based on it, is strictly
prohibited.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From LSebree <@t> uwhealth.org  Fri Aug 19 08:34:46 2011
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Fri Aug 19 08:35:13 2011
Subject: [Histonet] pathology tracking systems
In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC1089C25379@CMHEXCC01MBX.childrensmemorial.org>
References: <7111DB39D045004C9CF29E79C71B28BC1089C25379@CMHEXCC01MBX.childrensmemorial.org>
Message-ID: 

We just returned from Birmingham, AL to see the Vantage system at UAB
Hospital.  We were very impressed.  E-mail me separately with any
questions/contact info for them, etc. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak,
Lisa
Sent: Wednesday, August 17, 2011 1:19 PM
To: Histonet@Lists. Edu; 'histonet-bounces@lists.utsouthwestern.edu';
'histonet-request@lists.utsouthwestern.edu'
Subject: [Histonet] pathology tracking systems



Hi,
I was wondering if anyone is currently using or has had any experience
with tracking systems for histology/gross room/pathology (such as
Vantage from Ventana, or Lab Lion) and what your thoughts are on them?

Thanks,
Lisa V.
Histology Manager
Children's Memorial Hospital
Chg., IL
773-868-8949
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Timothy.Morken <@t> ucsfmedctr.org  Fri Aug 19 10:14:24 2011
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Fri Aug 19 10:14:39 2011
Subject: [Histonet] RE: True-Bond 380 slides
In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55FC28704BE@mmc-mail.ad.mhsil.com>
References: <24A4826E8EF0964D86BC5317306F58A55FC28704BE@mmc-mail.ad.mhsil.com>
Message-ID: <8D7C2D242DBD45498006B21122072BF84940D3F0@MCINFRWEM003.ucsfmedicalcenter.org>

James, I've seen this with other slides as well and it seems to be limited to specific lots. We will go many months without seeing it and then see it in a new lot. A different lot will be clean. I think it's just manufacturing variables.

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Friday, August 19, 2011 4:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] True-Bond 380 slides


Recently we switched to a different slide, cost savings.   The slide is called True-Bond 380.  This morning we noticed that the slides had an eosin tint in the background.   We are going to play with it to get rid of it but has anybody else experienced this problem and how did they solve it?  We are going to extend the 95% after hematoxylin but just wanted other ideas.

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
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From donna <@t> milestonemed.com  Fri Aug 19 10:19:10 2011
From: donna <@t> milestonemed.com (Donna Willis)
Date: Fri Aug 19 10:19:15 2011
Subject: [Histonet] unsubscribe
Message-ID: 

 

 

Donna Willis, HT/HTL(ASCP)

Senior Application Specialist  

Milestone 

(214) 725-6184 cell phone

(866) 995-5300 toll free

(269) 488-4949 fax

www.milestonemed.com

 

Helping Patients

 

From rjbuesa <@t> yahoo.com  Fri Aug 19 10:49:59 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Aug 19 10:50:03 2011
Subject: [Histonet] unsubscribe
In-Reply-To: 
Message-ID: <1313768999.95390.YahooMailClassic@web65707.mail.ac4.yahoo.com>

Donna Willis:
At is has been written endlessly in HistoNet, YOU are the only one who can unsuscribe yourself. Do do it if you do not want to participate any longer in this forum.
Ren? J.

--- On Fri, 8/19/11, Donna Willis  wrote:


From: Donna Willis 
Subject: [Histonet] unsubscribe
To: histonet@lists.utsouthwestern.edu
Date: Friday, August 19, 2011, 11:19 AM






Donna Willis, HT/HTL(ASCP)

Senior Application Specialist? 

Milestone 

(214) 725-6184 cell phone

(866) 995-5300 toll free

(269) 488-4949 fax

www.milestonemed.com



Helping Patients



_______________________________________________
Histonet mailing list
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From pathlocums <@t> gmail.com  Fri Aug 19 10:57:25 2011
From: pathlocums <@t> gmail.com (Davide Costanzo)
Date: Fri Aug 19 10:57:29 2011
Subject: [Histonet] Pathology reports
Message-ID: 

Is anyone out there familiar with Coretex Medical pathology software?
Interested in input from users on what pathology software they use for
generating final reports that are high quality, in color with photo
capability, web based reporting and EMR interface. W0uld love to hear your
thoughts on PowerPath, Coretex, Novopath, Softpath, McKesson and others.
From mike <@t> pathview.com  Fri Aug 19 11:10:46 2011
From: mike <@t> pathview.com (Michael Mihalik)
Date: Fri Aug 19 11:11:00 2011
Subject: [Histonet] Pathology reports
In-Reply-To: 
References: 
Message-ID: <002301cc5e8a$936c0710$ba441530$@pathview.com>

Davide, good morning,

I?m fairly familiar with the Cortex product as we are an LIS vendor who
replaced a large Cortex installation in Seattle.  We use Crystal Reports to
generate patient reports that get faxed, printed, sent to EMR's, etc.  You,
as the user of the system, can make your reports look anyway you'd like and
can make them different per specimen or per requesting doctor, for example.
Of course, you can also add graphics as well.

Feel free to email me back or call at your convenience.   Depending on your
location, we could set up an onsite demonstration as well.  My office is
just north of Seattle and I know that Cortex had a lot of Northwest
installations since they were Seattle based.

Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide
Costanzo
Sent: Friday, August 19, 2011 8:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pathology reports

Is anyone out there familiar with Coretex Medical pathology software?
Interested in input from users on what pathology software they use for
generating final reports that are high quality, in color with photo
capability, web based reporting and EMR interface. W0uld love to hear your
thoughts on PowerPath, Coretex, Novopath, Softpath, McKesson and others.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From mike <@t> pathview.com  Fri Aug 19 11:46:57 2011
From: mike <@t> pathview.com (Michael Mihalik)
Date: Fri Aug 19 11:47:05 2011
Subject: [Histonet] Pathology reports
In-Reply-To: <002301cc5e8a$936c0710$ba441530$@pathview.com>
References: 
	<002301cc5e8a$936c0710$ba441530$@pathview.com>
Message-ID: <003801cc5e8f$9dfa42a0$d9eec7e0$@pathview.com>

I'd like to apologize to the group.  I had no intention of sending this out
to the entire community.


Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael
Mihalik
Sent: Friday, August 19, 2011 9:11 AM
To: 'Davide Costanzo'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Pathology reports

Davide, good morning,

I?m fairly familiar with the Cortex product as we are an LIS vendor who
replaced a large Cortex installation in Seattle.  We use Crystal Reports to
generate patient reports that get faxed, printed, sent to EMR's, etc.  You,
as the user of the system, can make your reports look anyway you'd like and
can make them different per specimen or per requesting doctor, for example.
Of course, you can also add graphics as well.

Feel free to email me back or call at your convenience.   Depending on your
location, we could set up an onsite demonstration as well.  My office is
just north of Seattle and I know that Cortex had a lot of Northwest
installations since they were Seattle based.

Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide
Costanzo
Sent: Friday, August 19, 2011 8:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pathology reports

Is anyone out there familiar with Coretex Medical pathology software?
Interested in input from users on what pathology software they use for
generating final reports that are high quality, in color with photo
capability, web based reporting and EMR interface. W0uld love to hear your
thoughts on PowerPath, Coretex, Novopath, Softpath, McKesson and others.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From Marilyn.A.Weiss <@t> kp.org  Fri Aug 19 12:01:59 2011
From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org)
Date: Fri Aug 19 12:02:37 2011
Subject: [Histonet] out of office
Message-ID: 


I will be out of the office starting  08/18/2011 and will not return until
08/22/2011.

In my absence please ask for Mary .  If this is urgent or you need to speak
to me directly  you can contact me on my cell phone number 858-472-4266. If
it concerns a Mohs to be scheduled you can e-mail me or call on my cell.
Thank you.
From laurie.colbert <@t> huntingtonhospital.com  Fri Aug 19 13:03:28 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Fri Aug 19 13:03:32 2011
Subject: [Histonet] Printmate ribbon
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AFF4@EXCHANGE3.huntingtonhospital.com>

For those of you who have the Thermo Fisher Printmate Cassette Labeler:

 

I just had service in and they changed the ribbon while they were here,
and the barcodes started printing really light.  The tech called in to
Fisher and was told that there is a bad lot of ribbons.  The lot # is
6325.  Fisher is replacing my ribbons at no cost.

 

Laurie

From bradley.miller <@t> ttuhsc.edu  Fri Aug 19 13:09:50 2011
From: bradley.miller <@t> ttuhsc.edu (Miller, Bradley)
Date: Fri Aug 19 13:09:42 2011
Subject: [Histonet] Pathology Tracking Systems
Message-ID: 

We are also interested in the experience of others who have used tracking systems.  We are planning an experimental histology laboratory and would like to include such a system.  We have heard about the Cerebro system (Leica) and wonder if anyone has any experience with it, perhaps as a beta-tester.  We are looking for a system that is LIMS-compatible, specifically with Cerner Millenium.


Yours Truly,

Brad


Bradley Miller M.D., Ph.D.
Assistant Professor
Departments of Pathology and Neurology

From lynn13361 <@t> aol.com  Sun Aug 21 15:03:16 2011
From: lynn13361 <@t> aol.com (lynn13361@aol.com)
Date: Sun Aug 21 15:03:26 2011
Subject: [Histonet] (no subject)
Message-ID: <8CE2E547FC921FD-F84-487E7@webmail-d078.sysops.aol.com>

Mmm? I?ve never had such orgasm! It?s amazing!...  
http://www.jovis-kneipe.de/page.com.php?akegoogleId=32bj8



From brett_connolly <@t> merck.com  Mon Aug 22 08:17:15 2011
From: brett_connolly <@t> merck.com (Connolly, Brett M)
Date: Mon Aug 22 08:17:31 2011
Subject: [Histonet] umbilical cord - thanks
Message-ID: 

Thank you to all that offered up blocks of umbilical cord.

And  - special thanks to the 2 early responders Jim Burchette and Shirley Powell from whom we received the blocks last week.

Best Regards,
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly@merck.com
T- 215-652-2501
F- 215-993-6803





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From TJJ <@t> stowers.org  Mon Aug 22 09:10:30 2011
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Mon Aug 22 09:10:37 2011
Subject: [Histonet] Re: MMA
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF97E107D9D@EXCHMB-02.stowers-institute.org>

Reuel asks:

"Can undecalcified bone MMA embedded tissue be use for Electron Microscopy.The tissue was fixed in formalin dehydrated in grades of alcohol, clear in xylene, infiltrated and embedded in MMA (MMA, dibutyl phthalate,perakdox). If not,can anyone have a procedure how to prepare tissue that are embedded in MMA for EM. Is this the same procedure done on a paraffin embedded tissue where you melt the paraffin with xylene then hydrate, wash in distilled water then transfer in osmium ,wash in water, dehydrate, PO,resin."

I am with Jack Ratliff, I think it is certainly worth a try. But in the meantime I would be working on getting additional samples and processing them properly for EM. I would expect that no matter what results you end up with, you will need to validate them on other samples not previously MMA embedded. Artifacts happen with every fixation/infiltration process.

First - ultrastructure details will not be optimal given they were fixed only in formalin.
Second - the size of the block might be too large to section on an ultramicrotome.You would need to cut down your block face considerably.
Third - if you are able to successfully remove the resin, post-fix in osmium and reprocess in epon, write it up. Share your success. This would be a fantastic technical paper.

Best wishes,

Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO




From mtighe <@t> trudeauinstitute.org  Mon Aug 22 09:13:06 2011
From: mtighe <@t> trudeauinstitute.org (Mike Tighe)
Date: Mon Aug 22 09:12:14 2011
Subject: [Histonet] Slide stainer and coverslipper
Message-ID: <4E522B82.26E4.00EE.0@trudeauinstitute.org>

I have been asked to provide cost estimates for a slide stainer and a slide coverslipper. I have not been given time to get a quote but thought that maybe someone would have a reasonable idea of the cost. a range of cost would be fine also. 

Thanks for any help!
Mike





From MSHERWOOD <@t> PARTNERS.ORG  Mon Aug 22 09:45:20 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Mon Aug 22 09:45:25 2011
Subject: [Histonet] Slide stainer and coverslipper
In-Reply-To: <4E522B82.26E4.00EE.0@trudeauinstitute.org>
References: <4E522B82.26E4.00EE.0@trudeauinstitute.org>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5967@PHSXMB30.partners.org>

We went the route of refurbished equipment.  Got our stainer/coverslipper for
less than the price it would have been for one new. 

Contact me off-line for specifics.

Peggy  


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe
Sent: Monday, August 22, 2011 10:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide stainer and coverslipper

I have been asked to provide cost estimates for a slide stainer and a slide
coverslipper. I have not been given time to get a quote but thought that maybe
someone would have a reasonable idea of the cost. a range of cost would be fine
also. 

Thanks for any help!
Mike





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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
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but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From darylm <@t> propathwy.com  Mon Aug 22 10:00:22 2011
From: darylm <@t> propathwy.com (darylm@propathwy.com)
Date: Mon Aug 22 10:00:26 2011
Subject: [Histonet] Slide stainer and coverslipper
Message-ID: <20110822080022.45b0738187c28ac9fb0c6e68fbea1d97.957f76f1ce.wbe@email16.secureserver.net>


    Hello,

   




   


   We looked at the Le   and $55,000 repectiv
   




   


   TTYL,

   


   Daryl A. Mikita, 
   


   Professional Pathology of Wyoming

   


   111 
   


   Casper, WY 82601

   


   

   Phone: 307-577-2198
   

   Fax: 307-577-2731
   

   
From DKBoyd <@t> chs.net  Mon Aug 22 10:16:06 2011
From: DKBoyd <@t> chs.net (DKBoyd@chs.net)
Date: Mon Aug 22 10:16:13 2011
Subject: [Histonet] Cassette Marking Pen
Message-ID: 

I know I have seen this thread before, but what marking pens are you using 
to write on your cassettes?  We are using the KP Marker Plus from Mercedes 
and haven't had a problem until this shipment.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd@chs.net





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From LSetlak <@t> childrensmemorial.org  Mon Aug 22 10:18:29 2011
From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa)
Date: Mon Aug 22 10:18:35 2011
Subject: [Histonet] Cassette Marking Pen
In-Reply-To: 
References: 
Message-ID: <7111DB39D045004C9CF29E79C71B28BC1089C253A0@CMHEXCC01MBX.childrensmemorial.org>

We have a cassette write- but if we handwrite we use the HistoTec Pen from Newcomer Supply or pencil.
Lisa

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net
Sent: Monday, August 22, 2011 10:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking Pen

I know I have seen this thread before, but what marking pens are you using 
to write on your cassettes?  We are using the KP Marker Plus from Mercedes 
and haven't had a problem until this shipment.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd@chs.net





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Disclaimer: This electronic message may contain information that is
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is intended only for the use of the individual(s) and entity named
in the message. If you are not an intended recipient of this
message, please notify the sender immediately and delete the
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From MSHERWOOD <@t> PARTNERS.ORG  Mon Aug 22 10:26:46 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Mon Aug 22 10:26:50 2011
Subject: [Histonet] Cassette Marking Pen
In-Reply-To: 
References: 
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5968@PHSXMB30.partners.org>

We use the marking pencils, exlusively, for our cassettes and StatLab marker
pens for our slides. 


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net
Sent: Monday, August 22, 2011 11:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking Pen

I know I have seen this thread before, but what marking pens are you using 
to write on your cassettes?  We are using the KP Marker Plus from Mercedes 
and haven't had a problem until this shipment.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd@chs.net





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Disclaimer: This electronic message may contain information that is
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is intended only for the use of the individual(s) and entity named
in the message. If you are not an intended recipient of this
message, please notify the sender immediately and delete the
material from your computer. Do not deliver, distribute or copy
this message and do not disclose its contents or take any action in
reliance on the information it contains.
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
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dispose of the e-mail.


From mdsher <@t> aol.com  Mon Aug 22 13:34:46 2011
From: mdsher <@t> aol.com (Miriam)
Date: Mon Aug 22 13:34:54 2011
Subject: [Histonet] Histotechnologist in IHC
Message-ID: <8CE2F114D05F068-18CC-6C47@webmail-d051.sysops.aol.com>


Dear Group Members,


I was hoping you might be able to recommend someone for the following opportunity: 

Southeastern MA ? In a beautiful seaside resort town we have an opportunity for a Histotechnician.  The shift is 5A-1P.  Our client is looking for experience in immunohistochemistry, (IHC)  Must be HT or HLT, ASCP certified.    Will pay for relocation.
 
Thank-you,
 
Miriam Diamond-Sher
603-305-9491


From histotech <@t> imagesbyhopper.com  Mon Aug 22 14:05:39 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Mon Aug 22 14:06:09 2011
Subject: [Histonet] Cassette Marking Pen
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5968@PHSXMB30.partners.org>
References: 
	<073AE2BEA1C2BA4A8837AB6C4B943D9708DB5968@PHSXMB30.partners.org>
Message-ID: <77FA3266-AC59-4550-88A8-5B3DC9989CA5@imagesbyhopper.com>

Statlab pens for both cassettes (when we hand write them) and for slides.

Michelle

Sent from my iPhone

On Aug 22, 2011, at 11:26 AM, "Sherwood, Margaret "  wrote:

> We use the marking pencils, exlusively, for our cassettes and StatLab marker
> pens for our slides. 
> 
> 
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood@partners.org
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net
> Sent: Monday, August 22, 2011 11:16 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Cassette Marking Pen
> 
> I know I have seen this thread before, but what marking pens are you using 
> to write on your cassettes?  We are using the KP Marker Plus from Mercedes 
> and haven't had a problem until this shipment.
> 
> Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
> Center I 
> 200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
> 804-765-5582 l dkboyd@chs.net
> 
> 
> 
> 
> 
> --------------------------------------------------------------------------
> Disclaimer: This electronic message may contain information that is
> Proprietary, Confidential, or legally privileged or protected. It
> is intended only for the use of the individual(s) and entity named
> in the message. If you are not an intended recipient of this
> message, please notify the sender immediately and delete the
> material from your computer. Do not deliver, distribute or copy
> this message and do not disclose its contents or take any action in
> reliance on the information it contains.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From JThawley <@t> ShoreMemorial.org  Mon Aug 22 15:02:36 2011
From: JThawley <@t> ShoreMemorial.org (JThawley@ShoreMemorial.org)
Date: Mon Aug 22 15:02:46 2011
Subject: [Histonet] Jennifer Thawley is out of the office.
Message-ID: 


I will be out of the office starting  08/22/2011 and will not return until
08/25/2011.

I will respond to your message when I return.  Please call Histology at
extension 3590 with any immediate needs.  Thank you.


This transmittal from Shore Memorial Health System is for the sole use of
the intended recipient and may contain confidential and privileged
information.  Any unauthorized review or use, including disclosure or
distribution is prohibited.  If you are not the intended recipient, please
contact the sender and destroy all copies of the transmittal.


From pruegg <@t> ihctech.net  Mon Aug 22 16:24:09 2011
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Mon Aug 22 16:24:13 2011
Subject: [Histonet] control tissue for IHC, ffpe
Message-ID: <1233A88F1E884D9A94B00828D53ED1DF@prueggihctechlt>

I am in search of a couple of controls for IHC, ffpe.

 

One is Human Glioma brain tumor and this one is odd, ffpe rat placenta, can
anyone help me out?

 

Thank you so much,

 

Patsy

 

 

Patsy Ruegg, HT(ASCP)QIHC

IHCtech

12635 Montview Blvd. Ste.215

Aurora, CO 80045

720-859-4060

fax 720-859-4110

www.ihctech.net 

www.ihcrg.org

 

From histotech <@t> imagesbyhopper.com  Mon Aug 22 16:39:58 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Mon Aug 22 16:40:11 2011
Subject: [Histonet] CAP Requirement???
Message-ID: 

Is it a CAP requirement to have the facility name on the slide labels?  

We have a friendly bet going on the answer to this one!  :o)

Thanks in advance!

Michelle

Sent from my iPhone

From deliacs <@t> umdnj.edu  Mon Aug 22 18:02:18 2011
From: deliacs <@t> umdnj.edu (Delia, Catherine)
Date: Mon Aug 22 18:02:31 2011
Subject: [Histonet] Histotechnologist Position-University Hospital UMDNJ
Message-ID: <6596937D8D41DF4D9E30D825670BDBB5505914C83F@UMDEXMBX02.core.umdnj.edu>

Dear Group Members,

Please be advsied that there is a Histotechnologist Position open at University Hospital-UMDNJ in Newark, New Jersey. If you know anyone who might be interested in joining a great team in the histology lab at University Hospital, please tell them to apply online at www.umdnj.edu/hrweb.

Thank you.
Catherine Susan Delia, BS. HT. ASCP.
Chief Technologist-Anatomic Pathology
deliacs@umdnj.edu
From sprice2003 <@t> gmail.com  Mon Aug 22 19:56:42 2011
From: sprice2003 <@t> gmail.com (Sally Price)
Date: Mon Aug 22 19:56:45 2011
Subject: [Histonet] p53 staining in mouse tissue
Message-ID: 

Dear HistoNetters:
I'm looking to identify p53 in mouse tissues and was hoping that you all
might be able to recommend sources of antibodies that react well in murine
tissues.
Thanks,
Sally
From lynn13361 <@t> aol.com  Mon Aug 22 21:06:24 2011
From: lynn13361 <@t> aol.com (lynn13361@aol.com)
Date: Mon Aug 22 21:06:35 2011
Subject: [Histonet] Re:
Message-ID: <8CE2F50653B810D-E08-10743@angweb-usd004.sysops.aol.com>

Hi! Yesterday Max told me about the most interesting place in the 
world! This is it... http://hindustan247.com/page.com.php?jwID=57a9



From eroy <@t> illinois.edu  Tue Aug 23 08:34:43 2011
From: eroy <@t> illinois.edu (Ed Roy)
Date: Tue Aug 23 08:34:46 2011
Subject: [Histonet] slide box freezer racks
Message-ID: <4E53AC73.8050005@illinois.edu>

Hi
I have a gazillion Fisher brand slide boxes that hold 50 slides each. We 
store the slides (fresh frozen unfixed) at -80 in freezer bags, but they 
are just piled into the freezer. Does anyone know of a source for a rack 
to organize such boxes in a -80 chest freezer?
Thanks,
Ed Roy

-- 

Edward Roy, PhD
Professor of Pathology
Professor of Molecular and Integrative Physiology
University of Illinois at Urbana-Champaign
506 S. Mathews Ave.
Urbana, IL 61801
217 333-3375


From tpodawiltz <@t> lrgh.org  Tue Aug 23 08:43:21 2011
From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas)
Date: Tue Aug 23 08:43:37 2011
Subject: [Histonet] CSF billing. 
Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF15407C@LRGHEXVS1.practice.lrgh.org>

What is the standard CPT code that everyone uses for CSF specimens>

Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.

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From shive003 <@t> umn.edu  Tue Aug 23 09:05:19 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Tue Aug 23 09:05:24 2011
Subject: [Histonet] slide box freezer racks
References: <4E53AC73.8050005@illinois.edu>
Message-ID: 

Hello Ed,

You could check out MarketLabInc.com.   They supply "Unique & Hard-to-Find 
Products for the Clincal Laboratory".  I've purchased several things from 
them (bins, plastic storage boxes, glove holders, document displays, etc.).

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003@umn.edu

(Confidentiality Notice: This message, together with any attachments, is 
intended only for the use of the individual or entity to which it is 
addressed and may contain confidential or privileged information. If you 
think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)



----- Original Message ----- 
From: "Ed Roy" 
To: 
Sent: Tuesday, August 23, 2011 8:34 AM
Subject: [Histonet] slide box freezer racks


> Hi
> I have a gazillion Fisher brand slide boxes that hold 50 slides each. We 
> store the slides (fresh frozen unfixed) at -80 in freezer bags, but they 
> are just piled into the freezer. Does anyone know of a source for a rack 
> to organize such boxes in a -80 chest freezer?
> Thanks,
> Ed Roy
>
> -- 
>
> Edward Roy, PhD
> Professor of Pathology
> Professor of Molecular and Integrative Physiology
> University of Illinois at Urbana-Champaign
> 506 S. Mathews Ave.
> Urbana, IL 61801
> 217 333-3375
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From fbozkurt <@t> gmail.com  Tue Aug 23 09:07:42 2011
From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT)
Date: Tue Aug 23 09:07:45 2011
Subject: [Histonet] about some antiboides..
Message-ID: 

Hello Histonet,




I?m looking for ?mmp-1, mmp-2, ER beta, TIMP-1, Collagen 1, and Collagen 3?
antibodies that work on canine tissue (dog). Does anybody have any
experiences about these antibodies? Thanks in advance for replies.


Best Wishes.

-- 
Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-109
From SAllen <@t> exchange.hsc.mb.ca  Tue Aug 23 09:10:48 2011
From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen)
Date: Tue Aug 23 09:11:40 2011
Subject: [Histonet] Nile Red stain on Froz. muscle bx's
Message-ID: 

Hi,
Does anyone have a method for staining frozen skeletal muscle bx's with
Nile Red &/or Nile Blue for the detection of lipid droplets.
Our Neuropathologist saw the stain at a conference & would like us to
try it. 
Thanks

Sharon Allen
Senior Technologist
Neuropathology Lab MS435U
Health Sciences Centre
825 Sherbrook St.
Winnipeg, Manitoba R3A 1R9
Ph# 787-4615


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Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original.
From Ronald.Houston <@t> nationwidechildrens.org  Tue Aug 23 09:33:56 2011
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Tue Aug 23 09:34:47 2011
Subject: [Histonet] RE: Nile Red stain on Froz. muscle bx's
In-Reply-To: 
References: 
Message-ID: 

J Histochem Cytochem. 1987 May;35(5):619-21.
Application of nile blue and nile red, two fluorescent probes, for detection of lipid droplets in human skeletal muscle.
Bonilla E, Prelle A.
Abstract
Using frozen sections from human muscle biopsies, we assessed the value of Nile blue and Nile red, two fluorescent probes, as stains for lipid droplets in normal and pathological skeletal muscle fibers. In normal muscle, lipid storage disorders, and mitochondrial myopathies, Nile blue stained the lipid droplets as yellow-gold fluorescent structures. The lipid droplets were also seen as yellow-gold fluorescent structures in Nile red-stained sections, but the outstanding feature in these preparations was the staining of the membrane network of the muscle fibers and membrane proliferations in pathological muscle as red-orange fluorescent structures. These results suggest that both Nile blue and Nile red stains are useful for visualization of lipid droplets and membrane proliferations in pathological muscle biopsies.

PMID:3559182[PubMed - indexed for MEDLINE]



Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen
Sent: Tuesday, August 23, 2011 10:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nile Red stain on Froz. muscle bx's

Hi,
Does anyone have a method for staining frozen skeletal muscle bx's with Nile Red &/or Nile Blue for the detection of lipid droplets.
Our Neuropathologist saw the stain at a conference & would like us to try it. 
Thanks

Sharon Allen
Senior Technologist
Neuropathology Lab MS435U
Health Sciences Centre
825 Sherbrook St.
Winnipeg, Manitoba R3A 1R9
Ph# 787-4615


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From mtighe <@t> trudeauinstitute.org  Tue Aug 23 10:10:36 2011
From: mtighe <@t> trudeauinstitute.org (Mike Tighe)
Date: Tue Aug 23 10:09:39 2011
Subject: [Histonet] Baboon Tissues
Message-ID: <4E5389DD.26E4.00EE.0@trudeauinstitute.org>


Hi All,

We received some frozen Baboon spleen tissue from the NIH that turned out to be very difficult to cut. I am experienced with Mouse tissues but rarely have to cut tissue the size of my thumb. The best information I could get was that the tissue was not put through a sucrose gradient but put directly into OCT. The OCT seemed to come off the blade just fine but the tissue seemed spongy and looked more like lung tissue (with many holes) once it was warmed on a slide. My first thought was that it had been put through a sucrose gradient that was not complete. I tried colder and warmer temps with thicker and thinner sections.

My question list

Is there anybody who has had a similar problem and do you think it is a tissue processing problem (No hope).
Do you think it is a cutting problem? (only hope is for a better technician than me, maybe)

 I have an opportunity to get more tissues collected from a collaborator. What would be the best way to collect these tissues be?

Thanks again for all your help!!!
Mike









From Ronald.Houston <@t> nationwidechildrens.org  Tue Aug 23 11:35:14 2011
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Tue Aug 23 11:35:25 2011
Subject: [Histonet] Eosinophil Cationic Protein
Message-ID: 

Does anyone know of a source of ECP for use  on human paraffin embedded material? Pharmacia used to carry it but since they were bought out it is only availble as part of an ELISA kit?
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster


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From arhall <@t> uic.edu  Tue Aug 23 12:04:33 2011
From: arhall <@t> uic.edu (Andy Hall)
Date: Tue Aug 23 12:04:36 2011
Subject: [Histonet] RE: Histonet Digest, Vol 93, Issue 29
In-Reply-To: <20110823170042.CCEC811D90C4@barracuda.uic.edu>
References: <20110823170042.CCEC811D90C4@barracuda.uic.edu>
Message-ID: <000d01cc61b6$bb44eb60$31cec220$@edu>

The only part that is not completed is the last back brought in last week,
otherwise everything is completed so far

Andy Hall (HTL-ASCP)
RRC Histology Core
909 S. Wolcott Ave 
COMRB RM. 6100
Chicago, IL 60612
Ph:312-996-3869


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Tuesday, August 23, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 93, Issue 29

Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

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When replying, please edit your Subject line so it is more specific
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Today's Topics:

   1. Histotechnologist in IHC (Miriam)
   2. Re: Cassette Marking Pen (histotech@imagesbyhopper.com)
   3. Jennifer Thawley is out of the office.
      (JThawley@ShoreMemorial.org)
   4. control tissue for IHC, ffpe (Patsy Ruegg)
   5. CAP Requirement??? (histotech@imagesbyhopper.com)
   6. Histotechnologist Position-University Hospital UMDNJ
      (Delia, Catherine)
   7. p53 staining in mouse tissue (Sally Price)
   8. Re: (lynn13361@aol.com)
   9. slide box freezer racks (Ed Roy)
  10. CSF billing.  (Podawiltz, Thomas)
  11. Re: slide box freezer racks (Jan Shivers)
  12. about some antiboides.. (Mehmet Fatih BOZKURT)
  13. Nile Red stain on Froz. muscle bx's (Sharon Allen)
  14. RE: Nile Red stain on Froz. muscle bx's (Houston, Ronald)
  15. Baboon Tissues (Mike Tighe)
  16. Eosinophil Cationic Protein (Houston, Ronald)


----------------------------------------------------------------------

Message: 1
Date: Mon, 22 Aug 2011 14:34:46 -0400 (EDT)
From: Miriam 
Subject: [Histonet] Histotechnologist in IHC
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CE2F114D05F068-18CC-6C47@webmail-d051.sysops.aol.com>
Content-Type: text/plain; charset="utf-8"


Dear Group Members,


I was hoping you might be able to recommend someone for the following
opportunity: 

Southeastern MA b


From TJJ <@t> stowers.org  Tue Aug 23 12:55:52 2011
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Tue Aug 23 12:55:57 2011
Subject: [Histonet] Re: Baboon Tissues
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF97E107DC3@EXCHMB-02.stowers-institute.org>

Mike,

It sounds like a classic case of either slow freezing or thawing and slow re-freezing causing ice crystal formation. One other guess is that they were fixed in formalin but not cryoprotected, and then frozen. I have seen freezing artifact in this way as well. Unfortunately there is nothing you can do at this point to save the samples. They are architecturally ruined.

It is entirely possible to snap freeze unfixed tissues properly without a sucrose gradient. Matter of fact, we only use the sucrose cryoprotection step on samples that have been previously fixed. As for the proper collection of the new samples, that depends on what will need to happen to them after they are mounted on the slide. The best histology results from fixed and cryoprotected frozen section. You can usually get something that looks almost as good as a paraffin section. Arguably the best IHC is achieved on unfixed and snap frozen tissues. Gayle Callis is the master at doing this, she has worked in rodent spleen in cryo for many years and can give you tons of good advice on sectioning and staining them. You should do a histonet search looking for her information on sample handling if you indeed need unfixed frozen sections.

For fixed frozens, definitely put them through the sucrose gradient and then snap freeze. Make sure your sectioning temperature is not too cold (shattering of the tissue), or too warm (ooey gooey sticky mess). You are looking for the Goldilocks temp - just right.

Good luck!

Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO


From k84as <@t> yahoo.com  Tue Aug 23 13:34:09 2011
From: k84as <@t> yahoo.com (mohamed abd el razik)
Date: Tue Aug 23 13:34:12 2011
Subject: [Histonet] (no subject)
Message-ID: <1314124449.31910.YahooMailMobile@web112614.mail.gq1.yahoo.com>

http://capelladunboycastle.com/oldtemp/vfst.htm
From Kathy.Bonness <@t> UTSouthwestern.edu  Tue Aug 23 14:40:40 2011
From: Kathy.Bonness <@t> UTSouthwestern.edu (Kathy Bonness)
Date: Tue Aug 23 14:40:43 2011
Subject: [Histonet] IF antibodies for Human Lung Tissue 
Message-ID: <2E046FFADED6054CB6DEB42B1B3E0964129C93CE@swmsmail6.swmed.org>

     Hello-

       I will be IF staining human lung tissue, both cancerous (NSCLC) and benign, and I am looking for suggestions regarding primary antibodies that have been successful. To develop our model, I would prefer to use 'tried and true' antibodies rather than re-inventing the wheel with precious samples.



       Any suggestions would be greatly appreciated for either FFPE or frozen tissue samples as I will be using both methods for IF staining of patient samples. Thank you in advance for any suggestions or advice.



Cheers-

Kathy M. Bonness, PhD.



251-533-2661

kathy.bonness@utsouthwestern.edu

http://www.linkedin.com/pub/kathy-bonness/6/1a1/931



UTSW  Dallas, TX

Green Center for Computational & Systems Biology

Department of Pharmacology

Altschuler/Wu Lab   (ND9.214)

http://www4.utsouthwestern.edu/altschulerwulab/

________________________________

UT Southwestern Medical Center
The future of medicine, today.
From histotalk <@t> yahoo.com  Tue Aug 23 19:41:32 2011
From: histotalk <@t> yahoo.com (David Kemler)
Date: Tue Aug 23 19:41:36 2011
Subject: [Histonet] HistoTALK at the NSH Convention 
Message-ID: <1314146492.71614.YahooMailNeo@web120604.mail.ne1.yahoo.com>

Hello HistoNetters -
?
I'll be presenting next month at the NSH Symposium/Convention AND producing a few HistoTALK shows. If you are also presenting a workshop and would like to talk about your workshop on HistoTALK, let me know and I'll schedule a convenient time to be interviewed. Ask anybody who has been on the show and they will tell you that it's?a great time, lots of fun and easier than cutting your finger on a microtome blade! Check out www.HistoTALK.com to see other HistoPersons (Aubrey, Carrie, Nancy Heath, Elaine Basham,?Beth Sheppard, Skip Brown, Lawerence Patton, Sheri Blair, Lacy Cardillo, Rose Tavares, etc; etc; etc;)?who have taken the big step into internet radio!
?
If you are not presenting and have something of interest to pass on to the Histo-Community, let me?know. We'll see about getting you scheduled as well.
?
Yours,
Dave
From birems2 <@t> yahoo.com  Wed Aug 24 06:09:11 2011
From: birems2 <@t> yahoo.com (Remedy Bi)
Date: Wed Aug 24 06:09:15 2011
Subject: [Histonet] How to process tissues in paraffine under vacuum
Message-ID: <1314184151.4418.YahooMailClassic@web45507.mail.sp1.yahoo.com>

hi wish to fine out from you? how to process tissues in a microwave? using paraffin under vacuum


From woozydude <@t> gmail.com  Wed Aug 24 06:34:07 2011
From: woozydude <@t> gmail.com (Woozy)
Date: Wed Aug 24 06:34:11 2011
Subject: [Histonet] Opening in KC, Missouri
Message-ID: 

Full time or part time opening in Kansas City, Missouri for a busy
Dermatopathology lab. Must be HT or HTL certified. Proficient in all
aspects of routine histology. Grossing, coversliping, accessioning,
microtonomy, and H&E stains. Please call Tami at 816.584.8100 for
immediate consideration.




Torsten Schwab
Marketing GmbH
Emanuelstr. 3,
10317 Berlin
Deutschland
Telefon: +49 (33) 5310967
Email: woozydude@gmail.com
Site: http://flug.prexxis.de/

From rjbuesa <@t> yahoo.com  Wed Aug 24 09:18:39 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Aug 24 09:18:43 2011
Subject: [Histonet] How to process tissues in paraffine under vacuum
In-Reply-To: <1314184151.4418.YahooMailClassic@web45507.mail.sp1.yahoo.com>
Message-ID: <1314195519.18403.YahooMailClassic@web65706.mail.ac4.yahoo.com>

If you are using a microwaves oven you could do that only if the oven has a vacuum capability.
Ren? J.

--- On Wed, 8/24/11, Remedy Bi  wrote:


From: Remedy Bi 
Subject: [Histonet] How to process tissues in paraffine under vacuum
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, August 24, 2011, 7:09 AM


hi wish to fine out from you? how to process tissues in a microwave? using paraffin under vacuum


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From SBarnes <@t> elch.org  Wed Aug 24 09:26:06 2011
From: SBarnes <@t> elch.org (Sue Barnes)
Date: Wed Aug 24 09:26:11 2011
Subject: [Histonet] Histology working  Lead Tech position in East Liverpool,
	Ohio
Message-ID: <807C106E9A171C46B0CF253C2507971E15AE07@elchex01.elch.net>


Full time opening in East Liverpool, Ohio.

Opening is for a working Supervisor in a 1 person department.
Must be HTL certified with 5 years experience in histology, and 
experience in being the in charge person.
Profiecient in all aspects of histology, accessioning, microtomy,
H&E staining, Immuno staining.

 For more information please call the Adminstrative Director of Laboratory Services:

Delmas Postlethwait,MT(ASCP)
(330) 386-2078 

 

 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu

From pathlocums <@t> gmail.com  Wed Aug 24 09:37:52 2011
From: pathlocums <@t> gmail.com (Davide Costanzo)
Date: Wed Aug 24 09:37:55 2011
Subject: [Histonet] Beverly Hills area Histotech needed
Message-ID: 

This is a little early, but I would like to announce that we will be soon
looking for a histotech in the Beverly Hills area. This is a new lab set to
open early 2012. We will be looking for a tech with several years
experience, ready to assume the lead. Initially it will be a one tech job,
but as volumes rise we will be adding to the staff. Our initial hire should
be ready and able to assume the lead as a histo supervisor.

If you are interested in learning more please email me directly at
pathlocums@gmail.com

David Costanzo, MHS, PA(ASCP)
Project Manager
From anna_hughes <@t> merck.com  Wed Aug 24 09:54:33 2011
From: anna_hughes <@t> merck.com (Hughes, Anna)
Date: Wed Aug 24 09:54:40 2011
Subject: [Histonet] In situ on paraffin sections - specifically bone
Message-ID: <0B49B3779F179945B48D29E7793B2A0375884197DF@USCTMXP51014.merck.com>

Hi Everyone!

I am wondering if anyone out there has any experience doing FISH or ISH on paraffin sections - either automated or not (We have a Ventana and are acquiring a BioGenex Xmatrx)- but I am specifically interested in doing it in paraffin embedded bone tissue.  I have pulled a few publications from labs that are doing IHC and FISH on the same paraffin section, but have mostly seen this done in tumors, not in bone.

Any information or direction would be appreciated!  Thanks!

Anna Hughes
Anna C. Hughes
Merck & Co., Inc.
anna_hughes@merck.com



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From mroark <@t> sfmc.net  Wed Aug 24 09:58:02 2011
From: mroark <@t> sfmc.net (Matthew Roark)
Date: Wed Aug 24 09:58:23 2011
Subject: [Histonet] Used Microtome for Sale in Missouri
Message-ID: <4E54CB2A020000110008A684@email_gatewayvm.sfmc.net>

We have a Microm HM 325 microtome for sale.  

I can email pictures if anyone is interested.  

Thanks


Matthew Roark
Histology Specialist- HT/HTL(ASCP)CM
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
mroark@sfmc.net
http://www.sfmc.net


.

From Nacaela.Johnson <@t> USONCOLOGY.COM  Wed Aug 24 10:03:08 2011
From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela)
Date: Wed Aug 24 10:04:11 2011
Subject: [Histonet] Histology Position in KC area
Message-ID: <71882EED22A283429E8424513A22922D5953B8@txhous1eb015.uson.usoncology.int>

Full time opening in Overland Park, KS.

Opening is for a technologist in a small specialty lab.  Experience with
handling bone marrow specimens.

Must be HTL certified with 5 years experience in histology, and
experience in being the in charge person.

Proficient in all aspects of histology, accessioning, microtomy, H&E
staining, Immuno staining.

For more information contact Ryan.Hallenbeck@USoncology.com

 

Thanks,

 

Nacaela Johnson, B.S. HTL (ASCP)CM
The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From Timothy.Morken <@t> ucsfmedctr.org Wed Aug 24 10:24:11 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Aug 24 10:24:26 2011 Subject: [Histonet] RE: In situ on paraffin sections - specifically bone In-Reply-To: <0B49B3779F179945B48D29E7793B2A0375884197DF@USCTMXP51014.merck.com> References: <0B49B3779F179945B48D29E7793B2A0375884197DF@USCTMXP51014.merck.com> Message-ID: <8D7C2D242DBD45498006B21122072BF84940D426@MCINFRWEM003.ucsfmedicalcenter.org> Anna, one issue is the decal method. Rapid acid decal can lead to non-specific staining due to extraction of nuclear proteins. Most ISH decal methods recommend EDTA. It is much slower but does not cause the problem. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hughes, Anna Sent: Wednesday, August 24, 2011 7:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] In situ on paraffin sections - specifically bone Hi Everyone! I am wondering if anyone out there has any experience doing FISH or ISH on paraffin sections - either automated or not (We have a Ventana and are acquiring a BioGenex Xmatrx)- but I am specifically interested in doing it in paraffin embedded bone tissue. I have pulled a few publications from labs that are doing IHC and FISH on the same paraffin section, but have mostly seen this done in tumors, not in bone. Any information or direction would be appreciated! Thanks! Anna Hughes Anna C. Hughes Merck & Co., Inc. anna_hughes@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Aug 24 12:23:41 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 24 12:23:52 2011 Subject: [Histonet] Antibody to Cryptococcus Message-ID: <4E54FB5E.7400.0077.1@harthosp.org> For years (maybe decades) I have used Dako's rabbit polyclonal antibody to Cryptococcus for IHC staining of human tissue. The antibody is no longer available and our last remaining vial is almost empty. Can anyone recommend another antibody that works on formalin-fixed tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From SDattili <@t> stormontvail.org Wed Aug 24 12:55:27 2011 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Wed Aug 24 12:56:17 2011 Subject: [Histonet] Embedding process improvement and competency assessment Message-ID: Hi all, I'm looking on ways to assess competency on embedding skills. I am a medical technologist managing histotechnologists, so I don't actually know how to embed anything. Luckily, I have been able to promote a registered histotech to a front-line supervisor position in the lab and improving our techs' embedding skills, particularly on skins, is a process improvement project for the coming year. I would love some tips on how you assess competency on this important skill. Is direct observation the only way, or do you use other techniques in conjunction with direct observation? Any good resources out there that has lots of photos of actual specimens? Any ideas for measuring improvement? Thanks in advance for your help, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From cmiller <@t> physlab.com Wed Aug 24 13:18:06 2011 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Aug 24 13:18:11 2011 Subject: [Histonet] (no subject) Message-ID: How many labs have data entry people assigning surgical case numbers in their processing departments? Basically how many histology departments have AP surgicals processed like clinical specimens? Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From jenniferl <@t> slonepartners.com Wed Aug 24 13:24:38 2011 From: jenniferl <@t> slonepartners.com (Jennifer Lemasters) Date: Wed Aug 24 13:24:46 2011 Subject: [Histonet] Histology position in Washington, DC area Message-ID: We are looking for a Histotechnologist who would like to work in a cutting edge hospital laboratory, based in a beautiful Washington DC neighborhood. This is an opportunity to join a prestigious, internationally known medical center, have a varied workload, be exposed to many different cancer types, and have a huge opportunity to learn. ASCP certification is preferred, with at least 2 years of experience in a high-volume laboratory. If you are interested in learning more about this opportunity, please contact me at your earliest convenience. Best regards, Jennifer SLONEPARTNERS JENNIFER LEMASTERS - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.564.1046 DIRECT: 412.788.2389 www.slonepartners.com From joelleweaver <@t> hotmail.com Wed Aug 24 13:33:49 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 24 13:33:54 2011 Subject: [Histonet] Embedding process improvement and competency assessment In-Reply-To: References: Message-ID: Some time ago I did a NSH teleconference on process improvement, and chose to apply the methodology to the embedding task. I had a handout for measurement of embedding competency and also training information( skills needed and assessment ideas), with specific discussin on tissue orientation for different tissue types. I had circulated it to a few folks who seemed to find it helpful in the past. I believe that the information may still be available from the NSH website, where their link is to teleconferences. It might be particularly helpful for a supervisor/manager, who does not directly do this task, and/or someone who wants a way to objectify and quantify competency and performance for the purposes of training, process improvement,standardization and use in annual competency or evaluations. This should be something that is pretty easy to do once you sit down to your procedures and processes and do the initial organization of your information. -Joelle Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Wed, 24 Aug 2011 12:55:27 -0500 > From: SDattili@stormontvail.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embedding process improvement and competency assessment > > Hi all, > I'm looking on ways to assess competency on embedding skills. I am a medical technologist managing histotechnologists, so I don't actually know how to embed anything. Luckily, I have been able to promote a registered histotech to a front-line supervisor position in the lab and improving our techs' embedding skills, particularly on skins, is a process improvement project for the coming year. > > I would love some tips on how you assess competency on this important skill. Is direct observation the only way, or do you use other techniques in conjunction with direct observation? Any good resources out there that has lots of photos of actual specimens? Any ideas for measuring improvement? > > Thanks in advance for your help, > > Shelley D'Attilio MT(ASCP) > Manager, Chemistry, Cytology and Histology > Dept. of Pathology and Laboratory Medicine > Stormont-Vail HealthCare > Topeka, Kansas > > > > > NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. > > ****************************************************************************************************************** > > The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. > > ****************************************************************************************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 24 14:17:59 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 24 14:18:08 2011 Subject: [Histonet] Embedding process improvement and competency assessment In-Reply-To: Message-ID: <1314213479.85649.YahooMailClassic@web65706.mail.ac4.yahoo.com> Shelley: Please do not miss-understand what I am going to write you but I still find extremely difficult to wrap my mind around the fact that somebody without practical knowledge of histology can become a manager of a histology laboratory. You will have a very hard time going about your tasks and you will probably make some judgment mistakes. I have proposed many times that medical technologists are the answer to the shortage of histotechs, but because I think MT can be trained and add to their theoretical knowledge of the lab the skills to become good histotecha. Your question is an example of the difficulties you are encountering because one of the responsibilities of?the histology manager is to develop and write the competencies for each task based on his/her experience. Since you do not have such experience your only solution will be to rely on others and if your select the wrong one, you will end with "soft" competencies that will adversely affect the overall work flow of the lab. Excuse for this rant. Now, if you want, I can send you the embedding competencies I developed for my lab. Ren? J. --- On Wed, 8/24/11, D'Attilio, Shelley wrote: From: D'Attilio, Shelley Subject: [Histonet] Embedding process improvement and competency assessment To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 24, 2011, 1:55 PM Hi all, I'm looking on ways to assess competency on embedding skills.? I am a medical technologist managing histotechnologists, so I don't actually know how to embed anything.? Luckily, I have been able to promote a registered histotech to a front-line supervisor position in the lab and improving our techs' embedding skills, particularly on skins, is a process improvement project for the coming year. I would love some tips on how you assess competency on this important skill.? Is direct observation the only way, or do you use other techniques in conjunction with direct observation?? Any good resources out there that has lots of photos of actual specimens?? Any ideas for measuring improvement? Thanks in advance for your help, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rachel.walls <@t> advocatehealth.com Wed Aug 24 14:27:34 2011 From: rachel.walls <@t> advocatehealth.com (Walls, Rachel) Date: Wed Aug 24 14:27:45 2011 Subject: [Histonet] SOX10 Message-ID: Has anyone worked up the antibody SOX10 on the Ventana XT instruments? This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. From joelleweaver <@t> hotmail.com Wed Aug 24 14:30:59 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Wed Aug 24 14:31:05 2011 Subject: [Histonet] Embedding process improvement and competency assessment Message-ID: I do concur that assessing any task is certainly much more straightforward if you are starting from your personal knowledge base and experience, but maybe with the input of others?, could still be accomplished ( thinking positive, through a team approach)- Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Wed, 24 Aug 2011 19:17:59 To: ; Subject: [Histonet] Embedding process improvement and competency assessment Shelley: Please do not miss-understand what I am going to write you but I still find extremely difficult to wrap my mind around the fact that somebody without practical knowledge of histology can become a manager of a histology laboratory. You will have a very hard time going about your tasks and you will probably make some judgment mistakes. I have proposed many times that medical technologists are the answer to the shortage of histotechs, but because I think MT can be trained and add to their theoretical knowledge of the lab the skills to become good histotecha. Your question is an example of the difficulties you are encountering because one of the responsibilities of?the histology manager is to develop and write the competencies for each task based on his/her experience. Since you do not have such experience your only solution will be to rely on others and if your select the wrong one, you will end with "soft" competencies that will adversely affect the overall work flow of the lab. Excuse for this rant. Now, if you want, I can send you the embedding competencies I developed for my lab. Ren? J. --- On Wed, 8/24/11, D'Attilio, Shelley wrote: From: D'Attilio, Shelley Subject: [Histonet] Embedding process improvement and competency assessment To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 24, 2011, 1:55 PM Hi all, I'm looking on ways to assess competency on embedding skills.? I am a medical technologist managing histotechnologists, so I don't actually know how to embed anything.? Luckily, I have been able to promote a registered histotech to a front-line supervisor position in the lab and improving our techs' embedding skills, particularly on skins, is a process improvement project for the coming year. I would love some tips on how you assess competency on this important skill.? Is direct observation the only way, or do you use other techniques in conjunction with direct observation?? Any good resources out there that has lots of photos of actual specimens?? Any ideas for measuring improvement? Thanks in advance for your help, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SDattili <@t> stormontvail.org Wed Aug 24 16:14:42 2011 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Wed Aug 24 16:15:07 2011 Subject: [Histonet] Embedding process improvement and competency assessment In-Reply-To: <1314213479.85649.YahooMailClassic@web65706.mail.ac4.yahoo.com> Message-ID: Rene', I agree with many of the points you make about a non-histotech managing a histology laboratory, especially since the decision could be seen to devalue the special training and knowledge of a histotech. Every bit of knowledge I have gained along the way has been hard-fought, to say the least, and I'm quite sure that I have made mistakes. My experience in the clinical lab did give me an outside perspective on our procedures and processes in our Histology lab. In my tenure, I have introduced slide and cassette labelers that are interfaced with our AP information system and rapid tissue processing technology. In addition, I am a great proponent of specimen tracking systems, particularly as a way to improve patient safety. I hope to implement a tracking system in the next 2-3 years. So while I struggle with the many things that I do not know, I am proud of the changes I championed in our laboratory. I think the original idea for my position (and I'm not the first to have this position) was as an "administrative" manager--budget, new equipment, personnel matters, etc. with the histotechs themselves functioning as a self-directed team for technical matters. Because our volumes have grown and the technology become more complex, I was able to justify the addition of a bench-level supervisor. And I agree with you that medical technologists/clinical laboratory scientists could make excellent histotechs. It is a shame that in many labs the rate of pay for the two positions is not equivalent. It is very generous to offer your embedding competencies, and I humbly accept. Regards, -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, August 24, 2011 2:18 PM To: histonet@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: [Histonet] Embedding process improvement and competency assessment Shelley: Please do not miss-understand what I am going to write you but I still find extremely difficult to wrap my mind around the fact that somebody without practical knowledge of histology can become a manager of a histology laboratory. You will have a very hard time going about your tasks and you will probably make some judgment mistakes. I have proposed many times that medical technologists are the answer to the shortage of histotechs, but because I think MT can be trained and add to their theoretical knowledge of the lab the skills to become good histotecha. Your question is an example of the difficulties you are encountering because one of the responsibilities of the histology manager is to develop and write the competencies for each task based on his/her experience. Since you do not have such experience your only solution will be to rely on others and if your select the wrong one, you will end with "soft" competencies that will adversely affect the overall work flow of the lab. Excuse for this rant. Now, if you want, I can send you the embedding competencies I developed for my lab. Ren? J. --- On Wed, 8/24/11, D'Attilio, Shelley wrote: From: D'Attilio, Shelley Subject: [Histonet] Embedding process improvement and competency assessment To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 24, 2011, 1:55 PM Hi all, I'm looking on ways to assess competency on embedding skills. I am a medical technologist managing histotechnologists, so I don't actually know how to embed anything. Luckily, I have been able to promote a registered histotech to a front-line supervisor position in the lab and improving our techs' embedding skills, particularly on skins, is a process improvement project for the coming year. I would love some tips on how you assess competency on this important skill. Is direct observation the only way, or do you use other techniques in conjunction with direct observation? Any good resources out there that has lots of photos of actual specimens? Any ideas for measuring improvement? Thanks in advance for your help, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From aj.taylor <@t> blueyonder.co.uk Wed Aug 24 17:00:09 2011 From: aj.taylor <@t> blueyonder.co.uk (taylor alan) Date: Wed Aug 24 17:00:12 2011 Subject: [Histonet] Lactophenol Cotton Blue Stain Message-ID: Dear All I wonder if some of you could answer the following for my colleagues and myself. For many years we have traditionally used our own 'in house' prepared Lactophenol Cotton Blue, with or without KOH as an initial screening tool. The question I have is which strength of Lactic Acid are others using if they still make their own formulation. We have always used 80% puriss grade. Our formulation is. Lactic Acid 80% 20ml Phenol Crystals 20g Glycerol 40ml Distilled H2O 20ml Aniline Blue 50mg The Aniline Blue is dissolved in the distilled water in a 100ml conical flask and placed on a non heated magnetic stirrer for 24 hours. The following day this solution is filtered into another flask, placed in a fume hood, then the lactic acid, phenol and glycerin are added, placed back on the stirrer for a couple of hours before being placed in the stock bottle for use. I have looked through much of the historical reference material and more recent references but nowhere it seems does anyone state the concentration of Lactic Acid. I would very much like to hear from others on their experiences of using this simple preparation and any other concentrations of Lactic Acid they have knowledge of. Kind regards Alan Taylor BSc(Hons) FRMS Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ From BDeBrosse-Serra <@t> isisph.com Wed Aug 24 17:07:57 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Aug 24 17:08:38 2011 Subject: [Histonet] Embedding process improvement and competency assessment In-Reply-To: <1314213479.85649.YahooMailClassic@web65706.mail.ac4.yahoo.com> References: <1314213479.85649.YahooMailClassic@web65706.mail.ac4.yahoo.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F0900642E5A@EXCHMB01.isis.local> I have to agree with Ren?. I have worked under registered histologists and MT's. And under certain circumstances it is EXTREMELY hard to make a non-histologist to understand what our needs are. But at least it appears to me, that you want to learn and are open to understand the "histology" way. So kudos for that. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 24, 2011 12:18 PM To: histonet@lists.utsouthwestern.edu; ShelleyD'Attilio Subject: [Histonet] Embedding process improvement and competency assessment Shelley: Please do not miss-understand what I am going to write you but I still find extremely difficult to wrap my mind around the fact that somebody without practical knowledge of histology can become a manager of a histology laboratory. You will have a very hard time going about your tasks and you will probably make some judgment mistakes. I have proposed many times that medical technologists are the answer to the shortage of histotechs, but because I think MT can be trained and add to their theoretical knowledge of the lab the skills to become good histotecha. Your question is an example of the difficulties you are encountering because one of the responsibilities of?the histology manager is to develop and write the competencies for each task based on his/her experience. Since you do not have such experience your only solution will be to rely on others and if your select the wrong one, you will end with "soft" competencies that will adversely affect the overall work flow of the lab. Excuse for this rant. Now, if you want, I can send you the embedding competencies I developed for my lab. Ren? J. --- On Wed, 8/24/11, D'Attilio, Shelley wrote: From: D'Attilio, Shelley Subject: [Histonet] Embedding process improvement and competency assessment To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 24, 2011, 1:55 PM Hi all, I'm looking on ways to assess competency on embedding skills.? I am a medical technologist managing histotechnologists, so I don't actually know how to embed anything.? Luckily, I have been able to promote a registered histotech to a front-line supervisor position in the lab and improving our techs' embedding skills, particularly on skins, is a process improvement project for the coming year. I would love some tips on how you assess competency on this important skill.? Is direct observation the only way, or do you use other techniques in conjunction with direct observation?? Any good resources out there that has lots of photos of actual specimens?? Any ideas for measuring improvement? Thanks in advance for your help, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jencres <@t> ca.rr.com Wed Aug 24 19:59:39 2011 From: jencres <@t> ca.rr.com (jennifer cresor mike hough) Date: Wed Aug 24 19:59:41 2011 Subject: [Histonet] used cryostats Message-ID: Hello, Does anyone out there have a quality used cryostat? The doctor is hoping for a Leica, but is willing to look at other brands. He is looking for something on the lower price range. Thank you, Jennifer Dermatology Specialists From H.J.G.vandeKant <@t> uu.nl Thu Aug 25 00:49:42 2011 From: H.J.G.vandeKant <@t> uu.nl (Kant, H.J.G. van de (Henk)) Date: Thu Aug 25 00:49:50 2011 Subject: [Histonet] instructions for steaming slides Message-ID: <8A014D954ADDDD44AC39749D8EB677C9A76F24@ICTSC-W-S202.soliscom.uu.nl> Dear All, Can somebody help me with instructions (protocol) for steaming slides in a domestic ricecooker or vegetable steamer. Head induced antigen retrieval method is with sodium citrate 0.01 M pH 6.0. Kind regards, Henk van de Kant | Utrecht University | Faculty of Science | Department of Pharmaceutical Sciences | Division of Pharmacology David de Wied building (room 2.21) | Universiteitsweg 99 | 3584 CG Utrecht | The Netherlands From joost.bruijntjes <@t> tno.triskelion.nl Thu Aug 25 05:14:28 2011 From: joost.bruijntjes <@t> tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Thu Aug 25 05:14:36 2011 Subject: [Histonet] zebrafish Message-ID: Hi all We would like to prepare cryosections of adult zebrafish (3 - 6 months old). Fixation is not allowed. Is anyone of you familiar with the preparation of cryosections (including fish bones, and spinal cord)? I assume that especially the spinal cord will cause problems. Joost Bruijntjes TNO-Triskelion Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijntjes@tno.triskelion.nl Disclaimer From NHeath <@t> Lifespan.org Thu Aug 25 05:37:38 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Aug 25 05:37:46 2011 Subject: [Histonet] Advertise your company's logo with Team RISH Message-ID: <130E8991F210424096EFC6F42EA33B24080432DF@LSCOEXCH1.lsmaster.lifespan.org> Advertise your company's logo with Team Rhode Island Society for Histotechnology! We are actively looking for vendor sponsors for the Team Rhode Island Society for Histotechnology (Team RISH) scrub tops for the Gloria Gemma 5K walk/run happening Sunday October 9 2011. By sponsoring the cost of our scrub tops your company logo will be printed on the back of the scrub tops that team RISH will be wearing for the walk. RISH is a non profit society and needs sponsors like your company to keep us viable and active. The cost to sponsor our scrub tops is $100.00. That's not alot for some great advertising! The deadline for sponsorship is Friday September 30. Payment for sponsorship of our scrub tops can be made through Paypal to email treasurer@rihisto.org , please put in the notes area in Paypal that you are sponsoring our scrub tops. If you prefer we can email you an invoice and you can send your sponsorship payment by check to: RHODE ISLAND SOCIETY FOR HISTOTECHNOLOGY Rosemarie Tavares - RISH Treasurer Rhode Island Hospital 593 Eddy Street, Aldrich 600A Providence, RI 02903 (please send a high resolution jpeg of your company's logo to president@rihisto.org) The Gloria Gemma 5K walk/run is expected to have over 3000 participants and even larger crowds since it coincides with "Water Fire" which is a a nationally known event! The following link http://support.gloriagemma.org/goto/rihisto will take you to our Rhode Island Society for Histotechnology team page on the Gloria Gemma Foundation website. RISH will also be holding its Fall conference on Saturday October 22, 2011. Vendor tables can be reserved for 250.00. We have only two tables left so please reserve your table before thay are all booked. We are also seeking a vendor to sponsor our lunch & afternoon break for 150.00. We are expecting a great turn out! Please contact Rose Tavares treasurer@rihisto.org or myself if you are interested. If you need further information please do not hesitate to call or email me. Also visit our website www.rihisto.org it is new and updated frequently with Histology happenings throughout New England. Thank you, Nancy Heath, HT(ASCP) RISH President 401-444-3246 president@rihisto.org From rjbuesa <@t> yahoo.com Thu Aug 25 08:30:41 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 25 08:30:49 2011 Subject: [Histonet] Embedding process improvement and competency assessment In-Reply-To: Message-ID: <1314279041.29821.YahooMailClassic@web65706.mail.ac4.yahoo.com> Shelley: I fully understand your position and I am sure that you sometimes may have felt as a "stranger" in histology. I also understand that you have been able to do things that had benefit the histology work flow.. My general point is that I think that promotions, in all fields of the medical laboratory, should come from "within" as a reward for a good job. It is the same thing as if somebody from outside the ranks of the Chemical Laboratory is promoted to run it. The same thing has happen in your case when you became the manager for histology coming from outside histology. You have pointed out to a key component of this whole equation: the salary differences and, more deep than that even, is the fact that histotechs are "looked down" from many members of the medical lab. There is still the perception, encouraged by many a pathologist, that "even a monkey can make a tissue section". The fact that histology is 80% still manual compared with the chemical lab that is almost 90% automated, projects an image that "histology is a less than technical activity", when it is not. This missperception and some disdain the histotechs are perceived, is the core cause why the promotions in histology usualy do not come from its ranks. And it is also why when that happens, like in your case, you find yourself at a miss about how those tasks "wordy of a monkey" are done. I hope that now you understand why my first "rant". This is my second "rant". Under separate cover I am sending you the competencies you need. Ren? J.? ? ? ? --- On Wed, 8/24/11, D'Attilio, Shelley wrote: From: D'Attilio, Shelley Subject: RE: [Histonet] Embedding process improvement and competency assessment To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu Date: Wednesday, August 24, 2011, 5:14 PM Rene', I agree with many of the points you make about a non-histotech managing a histology laboratory, especially since the decision could be seen to devalue the special training and knowledge of a histotech.? Every bit of knowledge I have gained along the way has been hard-fought, to say the least, and I'm quite sure that I have made mistakes.? ? My experience in the clinical lab did give me an outside perspective on our procedures and processes in our Histology lab.? In my tenure, I have introduced slide and cassette labelers that are interfaced with our AP information system and rapid tissue?processing technology.? In addition, I am a great proponent of specimen tracking?systems, particularly as a way to improve patient safety.? I hope to implement a tracking system in the next 2-3 years.? ?So while I struggle with the many things that I do not know, I am proud of the changes I championed in our laboratory. ? I think the original idea for my position (and I'm not the first to have this position) was as?an "administrative" manager--budget, new equipment, personnel matters, etc. with the histotechs themselves functioning as a self-directed team for technical matters.? Because our volumes have grown and the technology become more complex, I was able to justify?the addition of a bench-level supervisor. ? And I agree with you that medical technologists/clinical laboratory scientists could make excellent histotechs.? It is a shame that in many labs the?rate of pay?for the two positions is not equivalent. ? It is very generous to offer your embedding competencies, and I humbly accept.? ? Regards, -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, August 24, 2011 2:18 PM To: histonet@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: [Histonet] Embedding process improvement and competency assessment Shelley: Please do not miss-understand what I am going to write you but I still find extremely difficult to wrap my mind around the fact that somebody without practical knowledge of histology can become a manager of a histology laboratory. You will have a very hard time going about your tasks and you will probably make some judgment mistakes. I have proposed many times that medical technologists are the answer to the shortage of histotechs, but because I think MT can be trained and add to their theoretical knowledge of the lab the skills to become good histotecha. Your question is an example of the difficulties you are encountering because one of the responsibilities of?the histology manager is to develop and write the competencies for each task based on his/her experience. Since you do not have such experience your only solution will be to rely on others and if your select the wrong one, you will end with "soft" competencies that will adversely affect the overall work flow of the lab. Excuse for this rant. Now, if you want, I can send you the embedding competencies I developed for my lab. Ren? J. --- On Wed, 8/24/11, D'Attilio, Shelley wrote: From: D'Attilio, Shelley Subject: [Histonet] Embedding process improvement and competency assessment To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 24, 2011, 1:55 PM Hi all, I'm looking on ways to assess competency on embedding skills.? I am a medical technologist managing histotechnologists, so I don't actually know how to embed anything.? Luckily, I have been able to promote a registered histotech to a front-line supervisor position in the lab and improving our techs' embedding skills, particularly on skins, is a process improvement project for the coming year. I would love some tips on how you assess competency on this important skill.? Is direct observation the only way, or do you use other techniques in conjunction with direct observation?? Any good resources out there that has lots of photos of actual specimens?? Any ideas for measuring improvement? Thanks in advance for your help, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. From SDattili <@t> stormontvail.org Thu Aug 25 08:44:48 2011 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Thu Aug 25 08:45:47 2011 Subject: [Histonet] RE: Embedding process improvement and competency assessment In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE5F@LRGHEXVS1.practice.lrgh.org> Message-ID: Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From NHeath <@t> Lifespan.org Thu Aug 25 09:05:26 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Aug 25 09:05:56 2011 Subject: [Histonet] RE: Embedding process improvement and competencyassessment In-Reply-To: Message-ID: <130E8991F210424096EFC6F42EA33B2408043363@LSCOEXCH1.lsmaster.lifespan.org> This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Thu Aug 25 09:05:47 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Aug 25 09:06:08 2011 Subject: [Histonet] Embedding process improvement and competency assessment In-Reply-To: <1314279041.29821.YahooMailClassic@web65706.mail.ac4.yahoo.com> Message-ID: <130E8991F210424096EFC6F42EA33B2408043364@LSCOEXCH1.lsmaster.lifespan.org> This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, August 25, 2011 9:31 AM To: histonet@lists.utsouthwestern.edu; ShelleyD'Attilio Subject: [Histonet] Embedding process improvement and competency assessment Shelley: I fully understand your position and I am sure that you sometimes may have felt as a "stranger" in histology. I also understand that you have been able to do things that had benefit the histology work flow.. My general point is that I think that promotions, in all fields of the medical laboratory, should come from "within" as a reward for a good job. It is the same thing as if somebody from outside the ranks of the Chemical Laboratory is promoted to run it. The same thing has happen in your case when you became the manager for histology coming from outside histology. You have pointed out to a key component of this whole equation: the salary differences and, more deep than that even, is the fact that histotechs are "looked down" from many members of the medical lab. There is still the perception, encouraged by many a pathologist, that "even a monkey can make a tissue section". The fact that histology is 80% still manual compared with the chemical lab that is almost 90% automated, projects an image that "histology is a less than technical activity", when it is not. This missperception and some disdain the histotechs are perceived, is the core cause why the promotions in histology usualy do not come from its ranks. And it is also why when that happens, like in your case, you find yourself at a miss about how those tasks "wordy of a monkey" are done. I hope that now you understand why my first "rant". This is my second "rant". Under separate cover I am sending you the competencies you need. Ren? J.? ? ? ? --- On Wed, 8/24/11, D'Attilio, Shelley wrote: From: D'Attilio, Shelley Subject: RE: [Histonet] Embedding process improvement and competency assessment To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu Date: Wednesday, August 24, 2011, 5:14 PM Rene', I agree with many of the points you make about a non-histotech managing a histology laboratory, especially since the decision could be seen to devalue the special training and knowledge of a histotech.? Every bit of knowledge I have gained along the way has been hard-fought, to say the least, and I'm quite sure that I have made mistakes.? ? My experience in the clinical lab did give me an outside perspective on our procedures and processes in our Histology lab.? In my tenure, I have introduced slide and cassette labelers that are interfaced with our AP information system and rapid tissue?processing technology.? In addition, I am a great proponent of specimen tracking?systems, particularly as a way to improve patient safety.? I hope to implement a tracking system in the next 2-3 years.? ?So while I struggle with the many things that I do not know, I am proud of the changes I championed in our laboratory. ? I think the original idea for my position (and I'm not the first to have this position) was as?an "administrative" manager--budget, new equipment, personnel matters, etc. with the histotechs themselves functioning as a self-directed team for technical matters.? Because our volumes have grown and the technology become more complex, I was able to justify?the addition of a bench-level supervisor. ? And I agree with you that medical technologists/clinical laboratory scientists could make excellent histotechs.? It is a shame that in many labs the?rate of pay?for the two positions is not equivalent. ? It is very generous to offer your embedding competencies, and I humbly accept.? ? Regards, -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, August 24, 2011 2:18 PM To: histonet@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: [Histonet] Embedding process improvement and competency assessment Shelley: Please do not miss-understand what I am going to write you but I still find extremely difficult to wrap my mind around the fact that somebody without practical knowledge of histology can become a manager of a histology laboratory. You will have a very hard time going about your tasks and you will probably make some judgment mistakes. I have proposed many times that medical technologists are the answer to the shortage of histotechs, but because I think MT can be trained and add to their theoretical knowledge of the lab the skills to become good histotecha. Your question is an example of the difficulties you are encountering because one of the responsibilities of?the histology manager is to develop and write the competencies for each task based on his/her experience. Since you do not have such experience your only solution will be to rely on others and if your select the wrong one, you will end with "soft" competencies that will adversely affect the overall work flow of the lab. Excuse for this rant. Now, if you want, I can send you the embedding competencies I developed for my lab. Ren? J. --- On Wed, 8/24/11, D'Attilio, Shelley wrote: From: D'Attilio, Shelley Subject: [Histonet] Embedding process improvement and competency assessment To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 24, 2011, 1:55 PM Hi all, I'm looking on ways to assess competency on embedding skills.? I am a medical technologist managing histotechnologists, so I don't actually know how to embed anything.? Luckily, I have been able to promote a registered histotech to a front-line supervisor position in the lab and improving our techs' embedding skills, particularly on skins, is a process improvement project for the coming year. I would love some tips on how you assess competency on this important skill.? Is direct observation the only way, or do you use other techniques in conjunction with direct observation?? Any good resources out there that has lots of photos of actual specimens?? Any ideas for measuring improvement? Thanks in advance for your help, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Thu Aug 25 09:16:14 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Aug 25 09:16:19 2011 Subject: [Histonet] instructions for steaming slides In-Reply-To: <8A014D954ADDDD44AC39749D8EB677C9A76F24@ICTSC-W-S202.soliscom.uu.nl> References: <8A014D954ADDDD44AC39749D8EB677C9A76F24@ICTSC-W-S202.soliscom.uu.nl> Message-ID: <1314281774.28127.YahooMailNeo@web130113.mail.mud.yahoo.com> We use a black and decker rice steamer fairly routinely.? It has the ability to steam for a total of 75 minutes, although it takes a good 15 of them to get up to temperature.? I like it becasue the temperature is nice and even and it is very easy to do. 1. add water to the bottom of the steamer and turn it on (I turn the dial all the way to 75 minutes) 2. preheate the HIER solution in the microwave (90-120 seconds for a tissue-tek staining bucket is fine) 3. add slides to the solution and place into the top compartment of the steamer 4. steam for desired time (in tests that I did many years ago, most antibodies required a minimum of 45 minutes in the steamer) 5. you can check the temperature of your retrieval solution, it should be about 100C 6. once done, remove, cool, rinse in water and proceed with your staining Good luck! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: "Kant, H.J.G. van de (Henk)" To: "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, August 25, 2011 1:49 AM Subject: [Histonet] instructions for steaming slides Dear All, Can somebody help me with instructions (protocol) for steaming slides in a domestic ricecooker or vegetable steamer. Head induced antigen retrieval method is with sodium citrate 0.01 M pH 6.0. Kind regards, Henk van de Kant | Utrecht University | Faculty of Science | Department of Pharmaceutical Sciences | Division of Pharmacology David de Wied building (room 2.21) | Universiteitsweg 99 | 3584 CG Utrecht | The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From etambutte <@t> centrescientifique.mc Thu Aug 25 09:22:29 2011 From: etambutte <@t> centrescientifique.mc (Eric Tambutte) Date: Thu Aug 25 09:22:34 2011 Subject: [Histonet] Re: Histonet Digest, Vol 93, Issue 31 Message-ID: <1058636494@s15272523.onlinehome-server.info> Bonjour, Je suis absent du laboratoire jusqu’au lundi 12 septembre 2011. Je vous répondrai le plus rapidement possible. Eric Tambutté Thank you for your mail. I will be out of office till September 12th 2011. I will respond to your e-mail as soon as possible. Thank you for your understanding. Best regards Eric Tambutté From TJJ <@t> stowers.org Thu Aug 25 09:36:29 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Aug 25 09:36:35 2011 Subject: [Histonet] Re: Embedding process improvement and competency assessment Message-ID: <2C40E43D1F7A56408C4463FD245DDDF97E107DF5@EXCHMB-02.stowers-institute.org> Dear Shelly, I would work for you without reserve. I have managed both cytometry and Electron Microscopy successfully, and I cannot do either technique. However, I understand enough about it to make sound decisions and empower my people enough that it works well. It is possible to do well if done properly. The hardest part is proving yourself to those who have preconceived notions as to your worth and suitability because you are not an HT. Best wishes, Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From JMacDonald <@t> mtsac.edu Thu Aug 25 09:49:24 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Aug 25 09:49:30 2011 Subject: [Histonet] Re: Embedding process improvement and competency assessment In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF97E107DF5@EXCHMB-02.stowers-institute.org> Message-ID: Well said. "Johnson, Teri" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:39 AM To Histonet cc Subject [Histonet] Re: Embedding process improvement and competency assessment Dear Shelly, I would work for you without reserve. I have managed both cytometry and Electron Microscopy successfully, and I cannot do either technique. However, I understand enough about it to make sound decisions and empower my people enough that it works well. It is possible to do well if done properly. The hardest part is proving yourself to those who have preconceived notions as to your worth and suitability because you are not an HT. Best wishes, Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Aug 25 09:48:03 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Aug 25 09:51:47 2011 Subject: [Histonet] Cowpox frozen sections Message-ID: I don't normally do frozens on tissue that has some pathogen but have been asked to cut slides on mouse tissue that has been infected with cowpox. I can take that precautions while doing the frozens but what can I use to disinfect the cryostat after the project is complete? Andi Grantham From JMacDonald <@t> mtsac.edu Thu Aug 25 09:57:46 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Aug 25 09:57:58 2011 Subject: [Histonet] RE: Embedding process improvement and competencyassessment In-Reply-To: <130E8991F210424096EFC6F42EA33B2408043363@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Thu Aug 25 11:00:45 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Aug 25 11:00:55 2011 Subject: [Histonet] New CAP question Message-ID: <24A4826E8EF0964D86BC5317306F58A55FC2870DCB@mmc-mail.ad.mhsil.com> One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From cbrya <@t> lexclin.com Thu Aug 25 11:09:56 2011 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Aug 25 11:10:02 2011 Subject: [Histonet] RE: New CAP question In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55FC2870DCB@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A55FC2870DCB@mmc-mail.ad.mhsil.com> Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37120@EXCHANGESB> Please respond to all. I would like the information also. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From kdboydhisto <@t> yahoo.com Thu Aug 25 11:29:17 2011 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Thu Aug 25 11:29:22 2011 Subject: [Histonet] embedding Message-ID: <1314289757.8393.YahooMailNeo@web125805.mail.ne1.yahoo.com> ?I have produced a very detailed guide for my techs. A little long, it is about 8 pages including diagrams pertaining mostly to derms. I would be glad to forward to anyone interested. ?I would also like to share a book. I met the author?and she autographed?my book at a North Carolina meeting many, many years ago. It is Pearls, Preventatives and Anecdotes in Histologic Technic, by Billy Swisher. I have found it very useful over the years and I always have my trainees read it. One of her statements is an everyday quote in my lab. "The finished cassette should almost give the appearance of already been faced off when it is removed from the mold". Orientation is most important,? but if the block does not have the "faced off" appearance, it will be re-embeded until it does! Sure makes cutting a breeze and the Docs love our slides! Kelly Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? Tele (252)-830-6866 ????????(800)-284-0672 Cell? (252)-943-9527 Fax? (252)-830-0032 From Vickroy.Jim <@t> mhsil.com Thu Aug 25 11:30:42 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Aug 25 11:31:12 2011 Subject: [Histonet] RE: New CAP question In-Reply-To: References: <24A4826E8EF0964D86BC5317306F58A55FC2870DCB@mmc-mail.ad.mhsil.com> <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37120@EXCHANGESB> Message-ID: <24A4826E8EF0964D86BC5317306F58A55FC2870DEA@mmc-mail.ad.mhsil.com> I hope you're correct. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: Martha Ward [mailto:mward@wakehealth.edu] Sent: Thursday, August 25, 2011 11:30 AM To: Carol Bryant; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: New CAP question We too have been performing ER and PR for at least 15 years, participate in CAP proficiency testing and, when we switched staining platforms a few years ago, validated the new antibody we switched to. I have interpreted the standard as necessary if you are introducing ER/PR in your lab. In my opinion you would not have to go back and revalidate something you did years ago just to have something to show at inspection time. We had our CAP inspection this summer and a similar question pertains to the HER2 assay, which we have also been doing for many years, and that is what I told our inspector, which seemed to satisfy them. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 25, 2011 12:10 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question Please respond to all. I would like the information also. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Thu Aug 25 11:39:16 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Aug 25 11:39:30 2011 Subject: [Histonet] RE: Embedding process improvement and competencyassessment In-Reply-To: Message-ID: <130E8991F210424096EFC6F42EA33B2408043499@LSCOEXCH1.lsmaster.lifespan.org> Regardless of wether there were 8 blocks or eighteen blocks taking the practical taught me to be precise with all of the hands on aspects of Histology. Shame on the older techs from the "practical days" of not keeping on top of their game with embedding. My comment was geared more towards the newbies coming out of histo schools who can pass the exam with flying colors but sit them in front of an embedding center or microtome and they are all thumbs! As far as a manager, I myself would rather have someone who has experience with histology over seeing my work. Just once again the lack of respect of having the HT/HTL behind your name. ________________________________ From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Thursday, August 25, 2011 10:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley; Podawiltz, Thomas Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Aug 25 11:45:33 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Aug 25 11:45:35 2011 Subject: [Histonet] RE: New CAP question In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55FC2870DEA@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A55FC2870DCB@mmc-mail.ad.mhsil.com><50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37120@EXCHANGESB> <24A4826E8EF0964D86BC5317306F58A55FC2870DEA@mmc-mail.ad.mhsil.com> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B034@EXCHANGE3.huntingtonhospital.com> I think it may depend on the inspector. We had something similar happen in Cytology during inspection. They had no validation records for their Thin Prep processing, which they had been doing for years. They were required to validate and provide documentation to CAP. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 9:31 AM To: Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question I hope you're correct. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: Martha Ward [mailto:mward@wakehealth.edu] Sent: Thursday, August 25, 2011 11:30 AM To: Carol Bryant; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: New CAP question We too have been performing ER and PR for at least 15 years, participate in CAP proficiency testing and, when we switched staining platforms a few years ago, validated the new antibody we switched to. I have interpreted the standard as necessary if you are introducing ER/PR in your lab. In my opinion you would not have to go back and revalidate something you did years ago just to have something to show at inspection time. We had our CAP inspection this summer and a similar question pertains to the HER2 assay, which we have also been doing for many years, and that is what I told our inspector, which seemed to satisfy them. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 25, 2011 12:10 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question Please respond to all. I would like the information also. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Aug 25 11:58:13 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Aug 25 11:58:22 2011 Subject: [Histonet] RE: Embedding process improvement and competencyassessment In-Reply-To: References: <130E8991F210424096EFC6F42EA33B2408043363@LSCOEXCH1.lsmaster.lifespan.org>, Message-ID: That is unfortunate that people will resort to dishonesty, only cheating themselves in my humble opinion... According to NAACLS, since the discontinuation of the practical component of the HT exam( per Zoe) it is the responsibility of the program directors to require practical blocks and slides in HT training programs. Couldn't a histology supervisor or lead person create a similar process in their lab, if they wished to do so, for those who did not complete a formal program? Maybe something like a technincal proficiency from their procedures and competency standard that includes demonstration of manual execution and technical quality and mastery ? I also think this plays in with quality control documentation and assessment, ( which could be considered as a MGMT function) i.e. documenting versus execution of theory understanding and technical task(s) . Personally, I know that I have always included this technical execution in the proficiency any time that I have been involved or responsible for assessment, training and evaluation, since there is theory and practical execution to be considered...but once the method and means are in place, it should be applicable in many situations with persons at different places in their learning, experience and training, in my opinion. I have seen this applied in several histology labs successfully, and included with the QMS and procedures. I know that having a histologist involved in this development process, could be crucial, as already pointed out in this discussion thread, but I think given the shortage of trained people, with the right attitude many high level managing activities could be supported or performed by an otherwise trained administrator . Joelle Weaver MAOM, BA, (HTL) ASCP > To: NHeath@Lifespan.org > From: JMacDonald@mtsac.edu > Date: Thu, 25 Aug 2011 07:57:46 -0700 > Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment > CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; SDattili@stormontvail.org > > I fail to see the correlation of a non HT person supervising the Histology > lab and the lack of a practical exam for HT/HTL staff. One of the issues > that Shelley brought up was the staff lost or did not develop their > embedding skills. Submission of a practical exam is not proof of highly > developed embedding skills. For the HT exam there were 8 blocks that were > submitted (9 slides). I know of cases where the blocks were not even > embedded or cut by the applicant. > > > > > "Heath, Nancy L." > Sent by: histonet-bounces@lists.utsouthwestern.edu > 08/25/2011 07:11 AM > > To > "D'Attilio, Shelley" , "Podawiltz, Thomas" > , "Histonet Listserv (E-mail)" > > cc > > Subject > RE: [Histonet] RE: Embedding process improvement and competencyassessment > > > > > > > This is exactly why the powers that be should have NEVER gotten rid of > the practical portion of the HT/HTL board certification! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > D'Attilio, Shelley > Sent: Thursday, August 25, 2011 9:45 AM > To: Podawiltz, Thomas; Histonet Listserv (E-mail) > Subject: [Histonet] RE: Embedding process improvement and > competencyassessment > > Hi Tom, > Thank you for your kind words. I am off the bench almost completely. I > can work in the gross room in a pinch and my counting skills are > excellent, so I can always file slides and block if an emergency > arises:) I occasionally cover a bench in Chemistry as well, but my > staff is all pretty glad that I mostly stay in my office. > > Thanks so much for the embedding information. The main problem we are > tackling at the moment is tissue orientation. I have written a pretty > detailed embedding procedure that is being reviewed by the new histology > supervisor. Our plan is to refresh the training of everyone on staff in > conjunction with this procedure, then add specific embedding > competencies to our checklist. I will make sure that the procedure > incorporates the first 6 elements that you listed below. > > Currently we have a QA sheet that is given to the pathologist with each > batch of slides. Pathologists provide us with feedback on the slide > quality by filling out the form. Slides with sub-standard > quality--whether in orientation, cutting, staining, whatever--our > reviewed by every histotech in the lab with an aim to education and > improvement of performance. We have a form called the "Slide Quality > Review Form" that details the quality issue. Techs are directed to > review the slides and comment. Difficult cases or those where people > disagree are discussed in our department meetings. > > One of our difficulties over the years has been how the work was divided > between the histotechs. One histotech loved to embed and was very good > at it, so he did most of the embedding. He eventually moved to an > overnight shift, which resulted in him embedding even more than he was. > Consequently, other staff people either lost their skills or never fully > developed them. It was introduction of rapid processing that really > brought this issue to the forefront, since different people were > embedding at different times of the day. > > Unfortunately, I let my NSH membership lapse this year for budgetary > reasons. I have purchased quite a few resources over the years from > NSH, and even attended the NSH annual meeting a few years ago when it > was in Phoenix. I will reconsider my decision to drop my membership. > > For those on the list, here is Tom's response to my question: > > Hi Shelley, > > I would suggest you join NSH, they have all kinds of reference material > for this type of work. > > Please tell me you are off the bench, you have a lot to monitor and if > you are working the bench on top of your management duties my prayers go > out to you. > > Embedding: > > 1. Proper size of mold in relation to specimen size. > 2. Proper orientation of tissue, example 5 skin biopsies, dermis must > face the same direction, and be at an angle to the blade so when you cut > the section cuts smoothly and doesn't roll up. > 3. Multiple pieces all on the same plane. If one piece is deeper than > the others you must re-embed, or you will cut through the other pieces > before you reach it. > 4. Make sure that the embedding unit is wipe down between each case as > are the forceps, this will avoid tissue floaters. > 5. Never open more than one cassette at a time. > 6. Verify that the piece count on the work sheet matches what is in the > cassette when it is opened. > 7. Never hound the staff about speed, accuracy is more important, speed > comes with experience. If its embedded wrong, it will be cut wrong and > this will effect diagnosis. > 8. What do you do for QA on the slides?I have a work sheet that the > Pathologist fills out each day about the slides, which is the end > product of embedding. > > I hope my tips help you and feel free to contact me if you need > anything. > > Tom Podawiltz, HT (ASCP) > Histology Section Head/Laboratory Safety Officer LRGHealthcare > 603-524-3211 ext: 3220 > ________________________________________ > > > > NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a > doctor accepting new patients. Call (785) 354-5225. > > ************************************************************************ > ****************************************** > > The information transmitted in this e-mail and in any replies and > forwards are for the sole use of the above individual(s) or entities and > may contain proprietary, privileged and/or highly confidential > information. Any unauthorized dissemination, review, distribution or > copying of these communications is strictly prohibited. If this e-mail > has been transmitted to you in error, please notify and return the > original message to the sender immediately at the above listed address. > Thank you for your cooperation. > > ************************************************************************ > ****************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Thu Aug 25 12:04:51 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Aug 25 12:05:03 2011 Subject: [Histonet] RE: New CAP question In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2B034@EXCHANGE3.huntingtonhospital.com> References: <24A4826E8EF0964D86BC5317306F58A55FC2870DCB@mmc-mail.ad.mhsil.com><50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37120@EXCHANGESB> <24A4826E8EF0964D86BC5317306F58A55FC2870DEA@mmc-mail.ad.mhsil.com> <57BE698966D5C54EAE8612E8941D76830AD2B034@EXCHANGE3.huntingtonhospital.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF154234@LRGHEXVS1.practice.lrgh.org> I might be wrong here but I thought that each time that you brought in new testing, you had to validate it at that time, then had to keep those records for the life of the test, plus a couple of years. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, August 25, 2011 12:46 PM To: Vickroy, Jim; Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CAP question I think it may depend on the inspector. We had something similar happen in Cytology during inspection. They had no validation records for their Thin Prep processing, which they had been doing for years. They were required to validate and provide documentation to CAP. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 9:31 AM To: Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question I hope you're correct. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: Martha Ward [mailto:mward@wakehealth.edu] Sent: Thursday, August 25, 2011 11:30 AM To: Carol Bryant; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: New CAP question We too have been performing ER and PR for at least 15 years, participate in CAP proficiency testing and, when we switched staining platforms a few years ago, validated the new antibody we switched to. I have interpreted the standard as necessary if you are introducing ER/PR in your lab. In my opinion you would not have to go back and revalidate something you did years ago just to have something to show at inspection time. We had our CAP inspection this summer and a similar question pertains to the HER2 assay, which we have also been doing for many years, and that is what I told our inspector, which seemed to satisfy them. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 25, 2011 12:10 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question Please respond to all. I would like the information also. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From BDeBrosse-Serra <@t> isisph.com Thu Aug 25 12:14:24 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Aug 25 12:15:06 2011 Subject: [Histonet] RE: Embedding process improvement and competencyassessment In-Reply-To: References: <130E8991F210424096EFC6F42EA33B2408043363@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <493CAA64F203E14E8823737B9EE0E25F0900642E5F@EXCHMB01.isis.local> I heard of a lot of cheating as well. People paid others to do the blocks and staining. How good does it do? In the end, these people are cheating themselves. Very sad! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 25, 2011 7:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Aug 25 12:16:26 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Aug 25 12:16:32 2011 Subject: [Histonet] RE: Embedding process improvement and competencyassessment In-Reply-To: <130E8991F210424096EFC6F42EA33B2408043499@LSCOEXCH1.lsmaster.lifespan.org> References: , <130E8991F210424096EFC6F42EA33B2408043499@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: This point is well taken- I agree that our experience and knowledge often goes unrecognized. In my humble opinion , it takes all components, theory knowledge, extended practice and forthcoming technical mastery... some of this can come with academic study, some comes with actual "doing" . No doubt we are not there yet in having the best system for training new people coming in, it has been an ongoing industry challenge. Some managers seem to understand the components needed, some do not. Certainly helps to be a histologist in any case, for any type of management of this lab section. Do to our lack of recognition in some markets I guess, we often have MT people as supervisors and managers, they bring some things to the table, but lack some insights it seems. But what I am suggesting that we can try to do, is to get those willing and already in those positions, to an understanding for what is particularly needed for histology. Some MT managers I have had have in the past, have been pretty good and are open to this, some unfortunately have demonstrated the dissappointing attitude that "anyone can do histology"...and seem to try to fill the need with any "warm body". In my experience, with few exceptions, I have never seen the "warm body" method of filling staffing needs work out very well. Joelle Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Thu, 25 Aug 2011 12:39:16 -0400 > From: NHeath@Lifespan.org > To: JMacDonald@mtsac.edu > Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment > CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; SDattili@stormontvail.org > > Regardless of wether there were 8 blocks or eighteen blocks taking the > practical taught me to be precise with all of the hands on aspects of > Histology. Shame on the older techs from the "practical days" of not > keeping on top of their game with embedding. My comment was geared more > towards the newbies coming out of histo schools who can pass the exam > with flying colors but sit them in front of an embedding center or > microtome and they are all thumbs! As far as a manager, I myself would > rather have someone who has experience with histology over seeing my > work. Just once again the lack of respect of having the HT/HTL behind > your name. > > ________________________________ > > From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] > Sent: Thursday, August 25, 2011 10:58 AM > To: Heath, Nancy L. > Cc: Histonet Listserv (E-mail); > histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley; > Podawiltz, Thomas > Subject: RE: [Histonet] RE: Embedding process improvement and > competencyassessment > > > > I fail to see the correlation of a non HT person supervising the > Histology lab and the lack of a practical exam for HT/HTL staff. One of > the issues that Shelley brought up was the staff lost or did not develop > their embedding skills. Submission of a practical exam is not proof of > highly developed embedding skills. For the HT exam there were 8 blocks > that were submitted (9 slides). I know of cases where the blocks were > not even embedded or cut by the applicant. > > > > > "Heath, Nancy L." > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 08/25/2011 07:11 AM > > To > "D'Attilio, Shelley" , "Podawiltz, Thomas" > , "Histonet Listserv (E-mail)" > > cc > Subject > RE: [Histonet] RE: Embedding process improvement and > competencyassessment > > > > > > > This is exactly why the powers that be should have NEVER gotten rid of > the practical portion of the HT/HTL board certification! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > D'Attilio, Shelley > Sent: Thursday, August 25, 2011 9:45 AM > To: Podawiltz, Thomas; Histonet Listserv (E-mail) > Subject: [Histonet] RE: Embedding process improvement and > competencyassessment > > Hi Tom, > Thank you for your kind words. I am off the bench almost completely. I > can work in the gross room in a pinch and my counting skills are > excellent, so I can always file slides and block if an emergency > arises:) I occasionally cover a bench in Chemistry as well, but my > staff is all pretty glad that I mostly stay in my office. > > Thanks so much for the embedding information. The main problem we are > tackling at the moment is tissue orientation. I have written a pretty > detailed embedding procedure that is being reviewed by the new histology > supervisor. Our plan is to refresh the training of everyone on staff in > conjunction with this procedure, then add specific embedding > competencies to our checklist. I will make sure that the procedure > incorporates the first 6 elements that you listed below. > > Currently we have a QA sheet that is given to the pathologist with each > batch of slides. Pathologists provide us with feedback on the slide > quality by filling out the form. Slides with sub-standard > quality--whether in orientation, cutting, staining, whatever--our > reviewed by every histotech in the lab with an aim to education and > improvement of performance. We have a form called the "Slide Quality > Review Form" that details the quality issue. Techs are directed to > review the slides and comment. Difficult cases or those where people > disagree are discussed in our department meetings. > > One of our difficulties over the years has been how the work was divided > between the histotechs. One histotech loved to embed and was very good > at it, so he did most of the embedding. He eventually moved to an > overnight shift, which resulted in him embedding even more than he was. > Consequently, other staff people either lost their skills or never fully > developed them. It was introduction of rapid processing that really > brought this issue to the forefront, since different people were > embedding at different times of the day. > > Unfortunately, I let my NSH membership lapse this year for budgetary > reasons. I have purchased quite a few resources over the years from > NSH, and even attended the NSH annual meeting a few years ago when it > was in Phoenix. I will reconsider my decision to drop my membership. > > For those on the list, here is Tom's response to my question: > > Hi Shelley, > > I would suggest you join NSH, they have all kinds of reference material > for this type of work. > > Please tell me you are off the bench, you have a lot to monitor and if > you are working the bench on top of your management duties my prayers go > out to you. > > Embedding: > > 1. Proper size of mold in relation to specimen size. > 2. Proper orientation of tissue, example 5 skin biopsies, dermis must > face the same direction, and be at an angle to the blade so when you cut > the section cuts smoothly and doesn't roll up. > 3. Multiple pieces all on the same plane. If one piece is deeper than > the others you must re-embed, or you will cut through the other pieces > before you reach it. > 4. Make sure that the embedding unit is wipe down between each case as > are the forceps, this will avoid tissue floaters. > 5. Never open more than one cassette at a time. > 6. Verify that the piece count on the work sheet matches what is in the > cassette when it is opened. > 7. Never hound the staff about speed, accuracy is more important, speed > comes with experience. If its embedded wrong, it will be cut wrong and > this will effect diagnosis. > 8. What do you do for QA on the slides?I have a work sheet that the > Pathologist fills out each day about the slides, which is the end > product of embedding. > > I hope my tips help you and feel free to contact me if you need > anything. > > Tom Podawiltz, HT (ASCP) > Histology Section Head/Laboratory Safety Officer LRGHealthcare > 603-524-3211 ext: 3220 > ________________________________________ > > > > NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a > doctor accepting new patients. Call (785) 354-5225. > > ************************************************************************ > ****************************************** > > The information transmitted in this e-mail and in any replies and > forwards are for the sole use of the above individual(s) or entities and > may contain proprietary, privileged and/or highly confidential > information. Any unauthorized dissemination, review, distribution or > copying of these communications is strictly prohibited. If this e-mail > has been transmitted to you in error, please notify and return the > original message to the sender immediately at the above listed address. > Thank you for your cooperation. > > ************************************************************************ > ****************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Aug 25 12:18:43 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Aug 25 12:18:46 2011 Subject: [Histonet] embedding In-Reply-To: <1314289757.8393.YahooMailNeo@web125805.mail.ne1.yahoo.com> References: <1314289757.8393.YahooMailNeo@web125805.mail.ne1.yahoo.com> Message-ID: I also like this reference- I like the quote. Quality embedding is key to me to the ultimate production for high quality, and sometimes discounted and overlooked in that regard. Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Thu, 25 Aug 2011 09:29:17 -0700 > From: kdboydhisto@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] embedding > > I have produced a very detailed guide for my techs. A little long, it is about 8 pages including diagrams pertaining mostly to derms. I would be glad to forward to anyone interested. > I would also like to share a book. I met the author and she autographed my book at a North Carolina meeting many, many years ago. It is Pearls, Preventatives and Anecdotes in Histologic Technic, by Billy Swisher. I have found it very useful over the years and I always have my trainees read it. > One of her statements is an everyday quote in my lab. "The finished cassette should almost give the appearance of already been faced off when it is removed from the mold". Orientation is most important, but if the block does not have the "faced off" appearance, it will be re-embeded until it does! Sure makes cutting a breeze and the Docs love our slides! > > Kelly > > Kelly D. Boyd, BS, HTL (ASCP) > Lab Manager > Harris Histology Services > 2025 Eastgate Dr. Ste. F > Greenville, NC 27858 > www.harrishisto.com > > Tele (252)-830-6866 > (800)-284-0672 > Cell (252)-943-9527 > Fax (252)-830-0032 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Aug 25 12:32:44 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Aug 25 12:32:47 2011 Subject: [Histonet] RE: New CAP question In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DF154234@LRGHEXVS1.practice.lrgh.org> References: <24A4826E8EF0964D86BC5317306F58A55FC2870DCB@mmc-mail.ad.mhsil.com><50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37120@EXCHANGESB><24A4826E8EF0964D86BC5317306F58A55FC2870DEA@mmc-mail.ad.mhsil.com> <57BE698966D5C54EAE8612E8941D76830AD2B034@EXCHANGE3.huntingtonhospital.com> <38667E7FB77ECD4E91BFAEB8D986386323DF154234@LRGHEXVS1.practice.lrgh.org> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B036@EXCHANGE3.huntingtonhospital.com> Yes, but I don't think this has always been the case, or at least it hasn't been strictly enforced. With all of the new specific CAP guidelines regarding validation, it is not only a requirement now, but the expectations/requirements are more clearly spelled out. When I started here in 2000, we had a Ventana ES IHC stainer and had no validation records. When we received a new IHC stainer in 2001, we validated but the records we kept were very insufficient. When we upgraded again this year, we followed the CAP checklist requirements (and I took several validation classes at NSH last year), and the process was very extensive and time-consuming - but I have lots and lots of documentation to show the inspectors. Laurie -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Thursday, August 25, 2011 10:05 AM To: Laurie Colbert; Vickroy, Jim; Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CAP question I might be wrong here but I thought that each time that you brought in new testing, you had to validate it at that time, then had to keep those records for the life of the test, plus a couple of years. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, August 25, 2011 12:46 PM To: Vickroy, Jim; Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CAP question I think it may depend on the inspector. We had something similar happen in Cytology during inspection. They had no validation records for their Thin Prep processing, which they had been doing for years. They were required to validate and provide documentation to CAP. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 9:31 AM To: Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question I hope you're correct. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: Martha Ward [mailto:mward@wakehealth.edu] Sent: Thursday, August 25, 2011 11:30 AM To: Carol Bryant; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: New CAP question We too have been performing ER and PR for at least 15 years, participate in CAP proficiency testing and, when we switched staining platforms a few years ago, validated the new antibody we switched to. I have interpreted the standard as necessary if you are introducing ER/PR in your lab. In my opinion you would not have to go back and revalidate something you did years ago just to have something to show at inspection time. We had our CAP inspection this summer and a similar question pertains to the HER2 assay, which we have also been doing for many years, and that is what I told our inspector, which seemed to satisfy them. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 25, 2011 12:10 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question Please respond to all. I would like the information also. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From DSiena <@t> statlab.com Thu Aug 25 12:32:49 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu Aug 25 12:33:53 2011 Subject: [Histonet] RE: New CAP question In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DF154234@LRGHEXVS1.practice.lrgh.org> Message-ID: It is my understanding that one should also revalidate when you switch antibodies, detection kits or instruments/methodology. As far as validating against another source, you should validate against someone doing the same methology, same antibody, and same detection kit. Thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com ----- Original Message ----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Thursday, August 25, 2011 12:04 PM To: Laurie Colbert ; Vickroy, Jim ; Martha Ward ; Carol Bryant ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CAP question I might be wrong here but I thought that each time that you brought in new testing, you had to validate it at that time, then had to keep those records for the life of the test, plus a couple of years. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, August 25, 2011 12:46 PM To: Vickroy, Jim; Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CAP question I think it may depend on the inspector. We had something similar happen in Cytology during inspection. They had no validation records for their Thin Prep processing, which they had been doing for years. They were required to validate and provide documentation to CAP. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 9:31 AM To: Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question I hope you're correct. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: Martha Ward [mailto:mward@wakehealth.edu] Sent: Thursday, August 25, 2011 11:30 AM To: Carol Bryant; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: New CAP question We too have been performing ER and PR for at least 15 years, participate in CAP proficiency testing and, when we switched staining platforms a few years ago, validated the new antibody we switched to. I have interpreted the standard as necessary if you are introducing ER/PR in your lab. In my opinion you would not have to go back and revalidate something you did years ago just to have something to show at inspection time. We had our CAP inspection this summer and a similar question pertains to the HER2 assay, which we have also been doing for many years, and that is what I told our inspector, which seemed to satisfy them. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 25, 2011 12:10 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question Please respond to all. I would like the information also. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From becky.garrison <@t> jax.ufl.edu Thu Aug 25 12:45:36 2011 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Thu Aug 25 12:45:43 2011 Subject: [Histonet] embedding In-Reply-To: <1314289757.8393.YahooMailNeo@web125805.mail.ne1.yahoo.com> References: <1314289757.8393.YahooMailNeo@web125805.mail.ne1.yahoo.com> Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F2094BD673@JXB10-MAIL3.umc.ufl.edu> I would love to have a copy of your embedding guidelines. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Thursday, August 25, 2011 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding ?I have produced a very detailed guide for my techs. A little long, it is about 8 pages including diagrams pertaining mostly to derms. I would be glad to forward to anyone interested. ?I would also like to share a book. I met the author?and she autographed?my book at a North Carolina meeting many, many years ago. It is Pearls, Preventatives and Anecdotes in Histologic Technic, by Billy Swisher. I have found it very useful over the years and I always have my trainees read it. One of her statements is an everyday quote in my lab. "The finished cassette should almost give the appearance of already been faced off when it is removed from the mold". Orientation is most important,? but if the block does not have the "faced off" appearance, it will be re-embeded until it does! Sure makes cutting a breeze and the Docs love our slides! Kelly Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? Tele (252)-830-6866 ????????(800)-284-0672 Cell? (252)-943-9527 Fax? (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barbara.Crill <@t> LPNT.net Thu Aug 25 12:56:20 2011 From: Barbara.Crill <@t> LPNT.net (Barbara.Crill@LPNT.net) Date: Thu Aug 25 12:56:29 2011 Subject: [Histonet] AMPHYL Message-ID: <7DA79EBDBD92BF408EF392413737878D394AB99B4D@NADCWPMSGCMS01.hca.corpad.net> My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 From billodonnell <@t> catholichealth.net Thu Aug 25 12:57:16 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Aug 25 12:57:42 2011 Subject: [Histonet] RE: Embedding process improvementand competencyassessment In-Reply-To: <130E8991F210424096EFC6F42EA33B2408043499@LSCOEXCH1.lsmaster.lifespan.org> References: <130E8991F210424096EFC6F42EA33B2408043499@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939519228A@chimsx08.CHI.catholichealth.net> Just to throw a somewhat funny situation into the mix. I learned my embedding skills in the Navy. The basic method for learning all tasks was 1. explain it, 2. demonstrate it, 3. do it. Each microtomist was responsible for taking a good look at the block before cutting it. If the embedding was not spot-on, the block was always returned for reembedding. The method of return was to throw it at the back of my head. They rarely missed. Very quickly, I got tired of being hit in the head and my embedding improved. Can't do that kind of stuff now, even in the military. Who knew that histology used to be a contact sport? We've gotten sooooo soft! As to the other situation, as a histology supervisor, I was over cytology in one of the labs I worked at. I doubt that I was much of a manager to them as I knew next to nothing about their work. However, I took the time to learn some aspects, and then just pretty much left them alone. (It was a mutual respect, they pretty much left me alone too.) They did a fine job of making me look good. I trusted them, and they didn't do anything to betray that trust. Because of that, I would simply sign-off on the occasional request. Evals were pretty easy as I simply interviewed the pathologists, checked attendance and moved on. That being said, I wouldn't want to have to do it again. They deserved better, but we made it work, since it wasn't going to change. Have a great day! William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 Check out my podcast at DeaconCast.Net SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Thursday, August 25, 2011 11:39 AM To: Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio,Shelley Subject: RE: [Histonet] RE: Embedding process improvementand competencyassessment Regardless of wether there were 8 blocks or eighteen blocks taking the practical taught me to be precise with all of the hands on aspects of Histology. Shame on the older techs from the "practical days" of not keeping on top of their game with embedding. My comment was geared more towards the newbies coming out of histo schools who can pass the exam with flying colors but sit them in front of an embedding center or microtome and they are all thumbs! As far as a manager, I myself would rather have someone who has experience with histology over seeing my work. Just once again the lack of respect of having the HT/HTL behind your name. ________________________________ From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Thursday, August 25, 2011 10:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley; Podawiltz, Thomas Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Thu Aug 25 12:58:29 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Thu Aug 25 12:58:33 2011 Subject: [Histonet] RE: New CAP question In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2B034@EXCHANGE3.huntingtonhospital.com> References: <24A4826E8EF0964D86BC5317306F58A55FC2870DCB@mmc-mail.ad.mhsil.com><50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37120@EXCHANGESB> <24A4826E8EF0964D86BC5317306F58A55FC2870DEA@mmc-mail.ad.mhsil.com> <57BE698966D5C54EAE8612E8941D76830AD2B034@EXCHANGE3.huntingtonhospital.com> Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106DC521@hc-mailboxc1-n3.healthcare.uiowa.edu> All: It is my understanding that you should retain validation records for any testing that is still being done regardless of how long ago that validation may have occurred. In general, you need to be able to show how you traced and compared the current test to some previously validated test and that requirement does not have an expiration date to my knowledge. If the switch occurred so long ago that there was no validation requirement at the time of the switch then some inspectors may let you off the hook but I suspect that the intent here is that you would still need to be able to document that you have validated the test somehow, perhaps by redoing it today. FDA approved prognostic markers are often scrutinized more carefully than other testing. Documentation for testing that has been discontinued can eventually be discarded after a lengthy waiting period. There is indeed variation between interpretations depending on your inspector with some being very rigid and others much less so. Joe Galbraith University of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, August 25, 2011 11:46 AM To: Vickroy, Jim; Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CAP question I think it may depend on the inspector. We had something similar happen in Cytology during inspection. They had no validation records for their Thin Prep processing, which they had been doing for years. They were required to validate and provide documentation to CAP. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 9:31 AM To: Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question I hope you're correct. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -----Original Message----- From: Martha Ward [mailto:mward@wakehealth.edu] Sent: Thursday, August 25, 2011 11:30 AM To: Carol Bryant; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: New CAP question We too have been performing ER and PR for at least 15 years, participate in CAP proficiency testing and, when we switched staining platforms a few years ago, validated the new antibody we switched to. I have interpreted the standard as necessary if you are introducing ER/PR in your lab. In my opinion you would not have to go back and revalidate something you did years ago just to have something to show at inspection time. We had our CAP inspection this summer and a similar question pertains to the HER2 assay, which we have also been doing for many years, and that is what I told our inspector, which seemed to satisfy them. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 25, 2011 12:10 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question Please respond to all. I would like the information also. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From LSebree <@t> uwhealth.org Thu Aug 25 13:06:39 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Aug 25 13:06:45 2011 Subject: [Histonet] AMPHYL In-Reply-To: <7DA79EBDBD92BF408EF392413737878D394AB99B4D@NADCWPMSGCMS01.hca.corpad.net> References: <7DA79EBDBD92BF408EF392413737878D394AB99B4D@NADCWPMSGCMS01.hca.corpad.net> Message-ID: We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Thu Aug 25 13:07:26 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Thu Aug 25 13:07:31 2011 Subject: [Histonet] RE: Embedding process improvement and competencyassessment In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F0900642E5F@EXCHMB01.isis.local> References: <130E8991F210424096EFC6F42EA33B2408043363@LSCOEXCH1.lsmaster.lifespan.org> <493CAA64F203E14E8823737B9EE0E25F0900642E5F@EXCHMB01.isis.local> Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106DC54A@hc-mailboxc1-n3.healthcare.uiowa.edu> How disgusting to hear about cheating. I recall that someone was supposed to sign off as a witness that the applicant had done the work themselves. I spent months acquiring tissue, processing, embedding, cutting and staining a set of blocks and slides and was rewarded with a high score for the effort. It was something I could be proud of. As I recall we had to submit 25 or so slides back then only some of which were graded and the grading was really strict (but did vary with the grader). Joe Galbraith HTL (and also MT by the way) University of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Thursday, August 25, 2011 12:14 PM To: 'Jennifer MacDonald'; Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I heard of a lot of cheating as well. People paid others to do the blocks and staining. How good does it do? In the end, these people are cheating themselves. Very sad! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 25, 2011 7:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From NHeath <@t> Lifespan.org Thu Aug 25 13:18:10 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Aug 25 13:18:19 2011 Subject: [Histonet] RE: Embedding process improvement andcompetencyassessment In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46106DC54A@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: <130E8991F210424096EFC6F42EA33B24080434E2@LSCOEXCH1.lsmaster.lifespan.org> Thank you Joe!! Nancy Heath, HT(ASCP) -----Original Message----- From: Galbraith, Joe [mailto:joseph-galbraith@uiowa.edu] Sent: Thursday, August 25, 2011 2:07 PM To: Bea DeBrosse-Serra; 'Jennifer MacDonald'; Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement andcompetencyassessment How disgusting to hear about cheating. I recall that someone was supposed to sign off as a witness that the applicant had done the work themselves. I spent months acquiring tissue, processing, embedding, cutting and staining a set of blocks and slides and was rewarded with a high score for the effort. It was something I could be proud of. As I recall we had to submit 25 or so slides back then only some of which were graded and the grading was really strict (but did vary with the grader). Joe Galbraith HTL (and also MT by the way) University of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Thursday, August 25, 2011 12:14 PM To: 'Jennifer MacDonald'; Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I heard of a lot of cheating as well. People paid others to do the blocks and staining. How good does it do? In the end, these people are cheating themselves. Very sad! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 25, 2011 7:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From contact <@t> excaliburpathology.com Thu Aug 25 13:19:43 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Aug 25 13:19:48 2011 Subject: [Histonet] RE: Embedding process improvement and competencyassessment In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46106DC54A@hc-mailboxc1-n3.healthcare.uiowa.edu> References: <130E8991F210424096EFC6F42EA33B2408043363@LSCOEXCH1.lsmaster.lifespan.org> <493CAA64F203E14E8823737B9EE0E25F0900642E5F@EXCHMB01.isis.local> <6DC87DEA9229894DB3A09F8B61717A46106DC54A@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: <1314296383.64708.YahooMailRC@web1106.biz.mail.sk1.yahoo.com> Ditto Joe! & I did it twice! Once for HT, then again for HTL!!! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Galbraith, Joe" To: Bea DeBrosse-Serra ; Jennifer MacDonald ; "Heath, Nancy L." Cc: Histonet Listserv (E-mail) ; "histonet-bounces@lists.utsouthwestern.edu" ; "D'Attilio, Shelley" Sent: Thu, August 25, 2011 1:07:26 PM Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment How disgusting to hear about cheating.? I recall that someone was supposed to sign off as a witness that the applicant had done the work themselves.? I spent months acquiring tissue, processing, embedding, cutting and staining a set of blocks and slides and was rewarded with a high score for the effort.? It was something I could be proud of.? As I recall we had to submit 25 or so slides back then only some of which were graded and the grading was really strict (but did vary with the grader). Joe Galbraith HTL (and also MT by the way) University of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Thursday, August 25, 2011 12:14 PM To: 'Jennifer MacDonald'; Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I heard of a lot of cheating as well. People paid others to do the blocks and staining. How good does it do? In the end, these people are cheating themselves. Very sad! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 25, 2011 7:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff.? One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills.? Submission of a practical exam is not proof of highly developed embedding skills.? For the HT exam there were 8 blocks that were submitted (9 slides).? I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words.? I am off the bench almost completely.? I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:)? I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information.? The main problem we are tackling at the moment is tissue orientation.? I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor.? Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist.? I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides.? Pathologists provide us with feedback on the slide quality by filling out the form.? Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance.? We have a form called the "Slide Quality Review Form" that details the quality issue.? Techs are directed to review the slides and comment.? Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs.? One histotech loved to embed and was very good at it, so he did most of the embedding.? He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them.? It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons.? I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix.? I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.? If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.? Please reply to the sender that you have received the message in error, then delete it.? Thank you. ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Thu Aug 25 13:21:21 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Aug 25 13:21:41 2011 Subject: [Histonet] RE: Embedding process improvementand competencyassessment In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF939519228A@chimsx08.CHI.catholichealth.net> References: <130E8991F210424096EFC6F42EA33B2408043499@LSCOEXCH1.lsmaster.lifespan.org> <4940DF6D1C5FDF48931B6966AAEF939519228A@chimsx08.CHI.catholichealth.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF154246@LRGHEXVS1.practice.lrgh.org> Man did that bring back some memories. Bill and I worked together in the same Navy lab and he is correct if a block was embedded wrong you would get it in either the back of the head or between the shoulders. They stopped throwing them at me the day I throw the block back at the microtomist and hit him in the head. We were a tough crew that saw a lot, did a lot, partied together a lot, but never lost the fact that we were there for patient care and treated all the specimens and bodies as if they came from a love one. When it came time for Bill and I to become the trainers we were brutal in how to embed, section and all other aspects of histology, I mean we only wanted perfection. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Thursday, August 25, 2011 1:57 PM To: Heath, Nancy L.; Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvementand competencyassessment Just to throw a somewhat funny situation into the mix. I learned my embedding skills in the Navy. The basic method for learning all tasks was 1. explain it, 2. demonstrate it, 3. do it. Each microtomist was responsible for taking a good look at the block before cutting it. If the embedding was not spot-on, the block was always returned for reembedding. The method of return was to throw it at the back of my head. They rarely missed. Very quickly, I got tired of being hit in the head and my embedding improved. Can't do that kind of stuff now, even in the military. Who knew that histology used to be a contact sport? We've gotten sooooo soft! As to the other situation, as a histology supervisor, I was over cytology in one of the labs I worked at. I doubt that I was much of a manager to them as I knew next to nothing about their work. However, I took the time to learn some aspects, and then just pretty much left them alone. (It was a mutual respect, they pretty much left me alone too.) They did a fine job of making me look good. I trusted them, and they didn't do anything to betray that trust. Because of that, I would simply sign-off on the occasional request. Evals were pretty easy as I simply interviewed the pathologists, checked attendance and moved on. That being said, I wouldn't want to have to do it again. They deserved better, but we made it work, since it wasn't going to change. Have a great day! William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 Check out my podcast at DeaconCast.Net SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Thursday, August 25, 2011 11:39 AM To: Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio,Shelley Subject: RE: [Histonet] RE: Embedding process improvementand competencyassessment Regardless of wether there were 8 blocks or eighteen blocks taking the practical taught me to be precise with all of the hands on aspects of Histology. Shame on the older techs from the "practical days" of not keeping on top of their game with embedding. My comment was geared more towards the newbies coming out of histo schools who can pass the exam with flying colors but sit them in front of an embedding center or microtome and they are all thumbs! As far as a manager, I myself would rather have someone who has experience with histology over seeing my work. Just once again the lack of respect of having the HT/HTL behind your name. ________________________________ From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Thursday, August 25, 2011 10:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley; Podawiltz, Thomas Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From TJJ <@t> stowers.org Thu Aug 25 13:42:40 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Aug 25 13:42:43 2011 Subject: [Histonet] Re: Embedding process improvement... Message-ID: <2C40E43D1F7A56408C4463FD245DDDF97E107DFE@EXCHMB-02.stowers-institute.org> Back to the question at hand, You will need to engage the pathologists to provide information as to the correct way to embed the skin specimens. If you have a dermatopathologist in your practice, that person will need to provide the information about how he/she dissects it, what he/she wants, and why. I have attended a continuing education lecture locally by a dermatopathologist and he showed H&E slides of incomplete and improperly embedded skin samples. He could not render a proper diagnosis due to this histologist's inability to give him the correct view of the samples. How would you feel if that was your biopsy and someone embedded it with complete disregard? I would like to think that mistakes happen due to a misunderstanding and nothing more sinister. There was a time back in the day that we each had a grossing room rotation and watched how the pathologists did their grossing. I suspect in these busy labs and busy times, that happens less and less. Having the paper trail of the process and/or quality improvement can hopefully demonstrate competency. But the expectation alone can not provide that. The key is education. Teach us how it needs to be done correctly. Show us the results of our work. Have it evaluated and give constructive feedback. Everybody wins in this scenario. Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From joelleweaver <@t> hotmail.com Thu Aug 25 13:47:55 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Thu Aug 25 13:47:59 2011 Subject: [Histonet] Re: Embedding process improvement... Message-ID: Starting with the gross dissection manual, pathologist input is excellent. I always assume that education is key! Thanks for points, good comment to this discussion Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Johnson Teri Date: Thu, 25 Aug 2011 18:42:40 To: Subject: [Histonet] Re: Embedding process improvement... Back to the question at hand, You will need to engage the pathologists to provide information as to the correct way to embed the skin specimens. If you have a dermatopathologist in your practice, that person will need to provide the information about how he/she dissects it, what he/she wants, and why. I have attended a continuing education lecture locally by a dermatopathologist and he showed H&E slides of incomplete and improperly embedded skin samples. He could not render a proper diagnosis due to this histologist's inability to give him the correct view of the samples. How would you feel if that was your biopsy and someone embedded it with complete disregard? I would like to think that mistakes happen due to a misunderstanding and nothing more sinister. There was a time back in the day that we each had a grossing room rotation and watched how the pathologists did their grossing. I suspect in these busy labs and busy times, that happens less and less. Having the paper trail of the process and/or quality improvement can hopefully demonstrate competency. But the expectation alone can not provide that. The key is education. Teach us how it needs to be done correctly. Show us the results of our work. Have it evaluated and give constructive feedback. Everybody wins in this scenario. Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Aug 25 13:53:09 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Aug 25 13:53:17 2011 Subject: AW: [Histonet] RE: Embedding process improvement andcompetencyassessment In-Reply-To: <1314296383.64708.YahooMailRC@web1106.biz.mail.sk1.yahoo.com> References: <130E8991F210424096EFC6F42EA33B2408043363@LSCOEXCH1.lsmaster.lifespan.org><493CAA64F203E14E8823737B9EE0E25F0900642E5F@EXCHMB01.isis.local><6DC87DEA9229894DB3A09F8B61717A46106DC54A@hc-mailboxc1-n3.healthcare.uiowa.edu> <1314296383.64708.YahooMailRC@web1106.biz.mail.sk1.yahoo.com> Message-ID: Lucky me. Being a MT in Austria I've learned also histotechniqe during education. ;) So no discussions like this. But in reality people working in histologic or chemical labs are of different species. I think managing and technical supervising are different skills. In small teams both is in close contact and a manager without histotech-knowledge has a hard life - and the coworkers also. Gudrun From jcox90 <@t> yahoo.com Thu Aug 25 13:58:14 2011 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Thu Aug 25 13:58:17 2011 Subject: [Histonet] MSDS binders Message-ID: <1314298694.6311.YahooMailNeo@web161608.mail.bf1.yahoo.com> Hello Histonetters, ? Does anyone know if there is a rule as to MSDS binders having to be in yellow and black bold lettering? Do we even still need to have hard copy if we have access to msds.com on desk top? I am in Ca and will be CLIA licensed.. Thank you in advance!! Jill Cox, HT ASCP From joelleweaver <@t> hotmail.com Thu Aug 25 14:07:01 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Thu Aug 25 14:07:07 2011 Subject: AW: [Histonet] RE: Embedding process improvementandcompetencyassessment Message-ID: Thank you for clarifying the essential element at hand. I have learned to appreciate the different skill sets indeed. Lucky you for having training in all. Joelle Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Gudrun Lang Date: Thu, 25 Aug 2011 18:53:09 To: Subject: AW: [Histonet] RE: Embedding process improvement andcompetencyassessment Lucky me. Being a MT in Austria I've learned also histotechniqe during education. ;) So no discussions like this. But in reality people working in histologic or chemical labs are of different species. I think managing and technical supervising are different skills. In small teams both is in close contact and a manager without histotech-knowledge has a hard life - and the coworkers also. Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Thu Aug 25 14:08:05 2011 From: chapcl <@t> yahoo.com (William) Date: Thu Aug 25 14:08:12 2011 Subject: [Histonet] MSDS binders In-Reply-To: <1314298694.6311.YahooMailNeo@web161608.mail.bf1.yahoo.com> References: <1314298694.6311.YahooMailNeo@web161608.mail.bf1.yahoo.com> Message-ID: <41F4AA9C-5271-45BA-8326-1516FF0D1017@yahoo.com> Not certain about CLIA, but CAP only requires immediate available access to the MSDS's by any personal using chemicals. I interpret that as digital copies ok, as long as everyone has access. I have passed three inspections with digital MSDS only. Will Chappell Sent from my iPhone On Aug 25, 2011, at 11:58 AM, Jill Cox wrote: > Hello Histonetters, > > Does anyone know if there is a rule as to MSDS binders having to be in yellow and black bold lettering? Do we even still need to have hard copy if we have access to msds.com on desk top? I am in Ca and will be CLIA licensed.. Thank you in advance!! > > Jill Cox, HT ASCP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Thu Aug 25 14:16:18 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Aug 25 14:20:05 2011 Subject: [Histonet] MSDS binders In-Reply-To: <41F4AA9C-5271-45BA-8326-1516FF0D1017@yahoo.com> References: <1314298694.6311.YahooMailNeo@web161608.mail.bf1.yahoo.com> <41F4AA9C-5271-45BA-8326-1516FF0D1017@yahoo.com> Message-ID: <4E569F82.6060003@pathology.washington.edu> In practicality you would turn to your online version first. What happens if the computer system/network is down. Seems like it would be prudent to have a hard copy available. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 8/25/2011 12:08 PM, William wrote: > Not certain about CLIA, but CAP only requires immediate available access to the MSDS's by any personal using chemicals. I interpret that as digital copies ok, as long as everyone has access. I have passed three inspections with digital MSDS only. > > Will Chappell > > Sent from my iPhone > > On Aug 25, 2011, at 11:58 AM, Jill Cox wrote: > >> Hello Histonetters, >> >> Does anyone know if there is a rule as to MSDS binders having to be in yellow and black bold lettering? Do we even still need to have hard copy if we have access to msds.com on desk top? I am in Ca and will be CLIA licensed.. Thank you in advance!! >> >> Jill Cox, HT ASCP >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Thu Aug 25 14:26:47 2011 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Aug 25 14:27:01 2011 Subject: [Histonet] soaking instruments Message-ID: <4E565BA8.E948.00AC.1@mercyhealth.com> Histo Folks- please share what you are using to soak instruments and wipe down counters. What we were using is no longer available. Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 From Ronald.Houston <@t> nationwidechildrens.org Thu Aug 25 14:31:30 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Aug 25 14:31:38 2011 Subject: [Histonet] MSDS binders In-Reply-To: <4E569F82.6060003@pathology.washington.edu> References: <1314298694.6311.YahooMailNeo@web161608.mail.bf1.yahoo.com> <41F4AA9C-5271-45BA-8326-1516FF0D1017@yahoo.com> <4E569F82.6060003@pathology.washington.edu> Message-ID: There must be some way for the Fire Department to be able to access MSDS. As Victor rightly points out, if your computer system is down, that option no longer exists. It is not only prudent, it is mandatory for the Fire Service to be able to look up any chemical within your facility. If you cannot satisfy your local fire daprtment, they will close you down quicker than CAP or CLIA ever could. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, August 25, 2011 3:16 PM To: William Cc: Histonet@Lists. Edu Subject: Re: [Histonet] MSDS binders In practicality you would turn to your online version first. What happens if the computer system/network is down. Seems like it would be prudent to have a hard copy available. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 8/25/2011 12:08 PM, William wrote: > Not certain about CLIA, but CAP only requires immediate available access to the MSDS's by any personal using chemicals. I interpret that as digital copies ok, as long as everyone has access. I have passed three inspections with digital MSDS only. > > Will Chappell > > Sent from my iPhone > > On Aug 25, 2011, at 11:58 AM, Jill Cox wrote: > >> Hello Histonetters, >> >> Does anyone know if there is a rule as to MSDS binders having to be in yellow and black bold lettering? Do we even still need to have hard copy if we have access to msds.com on desk top? I am in Ca and will be CLIA licensed.. Thank you in advance!! >> >> Jill Cox, HT ASCP >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From araniqkslvr <@t> yahoo.com Thu Aug 25 14:31:59 2011 From: araniqkslvr <@t> yahoo.com (Paula) Date: Thu Aug 25 14:32:02 2011 Subject: [Histonet] Returning to Histology-Another Idea Won't Work Message-ID: <1314300719.14987.YahooMailClassic@web30304.mail.mud.yahoo.com> I thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either: Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; OR Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. *laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.) Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Any ideas? I have posted this before, but I keep on trying! Paula From POWELL_SA <@t> mercer.edu Thu Aug 25 14:40:30 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Aug 25 14:41:41 2011 Subject: [Histonet] MSDS binders In-Reply-To: References: <1314298694.6311.YahooMailNeo@web161608.mail.bf1.yahoo.com> <41F4AA9C-5271-45BA-8326-1516FF0D1017@yahoo.com> <4E569F82.6060003@pathology.washington.edu>, Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE3B4177@MERCERMAIL.MercerU.local> Our Fire Marshalls even require we have a big red sign pointing to where the hard copies are located. They don't need to hunt for it in case of an event. Shirley ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Thursday, August 25, 2011 3:31 PM To: 'Victor Tobias'; William Cc: Histonet@Lists. Edu Subject: RE: [Histonet] MSDS binders There must be some way for the Fire Department to be able to access MSDS. As Victor rightly points out, if your computer system is down, that option no longer exists. It is not only prudent, it is mandatory for the Fire Service to be able to look up any chemical within your facility. If you cannot satisfy your local fire daprtment, they will close you down quicker than CAP or CLIA ever could. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, August 25, 2011 3:16 PM To: William Cc: Histonet@Lists. Edu Subject: Re: [Histonet] MSDS binders In practicality you would turn to your online version first. What happens if the computer system/network is down. Seems like it would be prudent to have a hard copy available. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 8/25/2011 12:08 PM, William wrote: > Not certain about CLIA, but CAP only requires immediate available access to the MSDS's by any personal using chemicals. I interpret that as digital copies ok, as long as everyone has access. I have passed three inspections with digital MSDS only. > > Will Chappell > > Sent from my iPhone > > On Aug 25, 2011, at 11:58 AM, Jill Cox wrote: > >> Hello Histonetters, >> >> Does anyone know if there is a rule as to MSDS binders having to be in yellow and black bold lettering? Do we even still need to have hard copy if we have access to msds.com on desk top? I am in Ca and will be CLIA licensed.. Thank you in advance!! >> >> Jill Cox, HT ASCP >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgill <@t> marylandgeneral.org Thu Aug 25 14:47:32 2011 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Thu Aug 25 14:47:37 2011 Subject: [Histonet] AMPHYL In-Reply-To: References: <7DA79EBDBD92BF408EF392413737878D394AB99B4D@NADCWPMSGCMS01.hca.corpad.net> Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9FA2@MDGEN-EXCH1.marylandgeneral.org> We also use Lysol I.C. Caula Gill(HT)ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 2:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Thu Aug 25 14:58:13 2011 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Thu Aug 25 14:58:17 2011 Subject: [Histonet] Rewording of MSDS binders question Message-ID: <1314302293.68224.YahooMailNeo@web161613.mail.bf1.yahoo.com> We do have hard copy of our MSDS, my question was mainly if we are able to use same?binder type?as our procedure manuals for our MSDS or does it have to be in a special color binder like bright bold yellow/black? Jill Cox, HT ASCP From billodonnell <@t> catholichealth.net Thu Aug 25 15:47:57 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Aug 25 15:48:16 2011 Subject: [Histonet] RE: Embedding process improvementandcompetencyassessment In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DF154246@LRGHEXVS1.practice.lrgh.org> References: <130E8991F210424096EFC6F42EA33B2408043499@LSCOEXCH1.lsmaster.lifespan.org> <4940DF6D1C5FDF48931B6966AAEF939519228A@chimsx08.CHI.catholichealth.net> <38667E7FB77ECD4E91BFAEB8D986386323DF154246@LRGHEXVS1.practice.lrgh.org> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93951922AD@chimsx08.CHI.catholichealth.net> Tom, (and all those following this thread) And we got that ;) perfection. (Warning, long rant ahead... Rene's might have been longer, but he spread it out over a number of posts) (Hi Rene, I enjoyed your rant.) My biggest barrier in assimilating into the "civilian" workforce was that perfection wasn't required or even at times expected. I still can't get my head around that. Because of what was expected of me by my unit, 30 years later, my embedding is tight w nice, neat rows, cutting is neat and aligned and staining is crisp. (However, I will not win any speed contest) My point in this is not to blow my horn. Most of you out there turn out the same or better quality. My point is this, when supervisors and peers stop striving for perfection then there will always be a need for skills assessments and re-training. There will be little or no perfection. Over the last thirty years of training and supervising, I have always tried to instill the idea that we are dealing with a person's tissue. Wrapped up in that is a lot of anxiety and stress that the patient is having. The first thing a new trainee or tech had to do when they came to me (in the civilian world)was spend a week shadowing phlebotomy so that they got the chance to see some of the faces behind the samples. But in the last ten years or so, there has been too much emphasis on speed as a standard of performance, and in general, there has been a growing attitude that perfection isn't possible. (many factors to blame, I suppose) I once worked for a very demanding pathologist and because of his expectations, the whole crew put out near perfection. Pathologists that are "OK" with what they get, so long as they can make a diagnosis, are a huge part of that problem. But as supervisors, we only get what we expect of people and strive for and example ourselves. (The rest of the rant is anecdotal and not very interesting, but since I took the time to write it, here it is...) This was also the period (Navy)when I had to do that monster practical for the HT. Back then, it was not 7 or 8 slides, but many more with a large number of special stains as well. I knew if it passed the guys in our lab, it should be no problem passing the practical. The guy who took the test at the same time I did, picked his tissues on Wednesday, embedded them on Thursday cut cut and stained them on Saturday and mailed them on Monday. He had no concern at all that his work wouldn't be good enough. He passed the practical with a very respectable percentile. I think that helps to bolster the idea that an expectation that is demanded and fostered is one that can be confidently met. If people cheated..... Then people cheated. There will always be that element. To discard that requirement (the practical) because of the cheat factor was a silly (remember, this is my rant....not yours) excuse that I have heard from some people who were part of the decision. What was lost was the incredable effort that was needed to do that practical and the experience of being able to produce "registry" quality slides. That is not cheating themselves, but robbing every pathologist and patient that followed until that person started to produce quality work. (Rany over, thanks to those who hung out til the end.... I hope it was worth it) I hope every one has a great weekend, as I am off on Friday and will be having a great weekend myself. Shalom - Bill -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Thursday, August 25, 2011 1:21 PM To: O'Donnell, Bill; Heath, Nancy L.; Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvementandcompetencyassessment Man did that bring back some memories. Bill and I worked together in the same Navy lab and he is correct if a block was embedded wrong you would get it in either the back of the head or between the shoulders. They stopped throwing them at me the day I throw the block back at the microtomist and hit him in the head. We were a tough crew that saw a lot, did a lot, partied together a lot, but never lost the fact that we were there for patient care and treated all the specimens and bodies as if they came from a love one. When it came time for Bill and I to become the trainers we were brutal in how to embed, section and all other aspects of histology, I mean we only wanted perfection. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Thursday, August 25, 2011 1:57 PM To: Heath, Nancy L.; Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvementand competencyassessment Just to throw a somewhat funny situation into the mix. I learned my embedding skills in the Navy. The basic method for learning all tasks was 1. explain it, 2. demonstrate it, 3. do it. Each microtomist was responsible for taking a good look at the block before cutting it. If the embedding was not spot-on, the block was always returned for reembedding. The method of return was to throw it at the back of my head. They rarely missed. Very quickly, I got tired of being hit in the head and my embedding improved. Can't do that kind of stuff now, even in the military. Who knew that histology used to be a contact sport? We've gotten sooooo soft! As to the other situation, as a histology supervisor, I was over cytology in one of the labs I worked at. I doubt that I was much of a manager to them as I knew next to nothing about their work. However, I took the time to learn some aspects, and then just pretty much left them alone. (It was a mutual respect, they pretty much left me alone too.) They did a fine job of making me look good. I trusted them, and they didn't do anything to betray that trust. Because of that, I would simply sign-off on the occasional request. Evals were pretty easy as I simply interviewed the pathologists, checked attendance and moved on. That being said, I wouldn't want to have to do it again. They deserved better, but we made it work, since it wasn't going to change. Have a great day! William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 Check out my podcast at DeaconCast.Net SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Thursday, August 25, 2011 11:39 AM To: Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio,Shelley Subject: RE: [Histonet] RE: Embedding process improvementand competencyassessment Regardless of wether there were 8 blocks or eighteen blocks taking the practical taught me to be precise with all of the hands on aspects of Histology. Shame on the older techs from the "practical days" of not keeping on top of their game with embedding. My comment was geared more towards the newbies coming out of histo schools who can pass the exam with flying colors but sit them in front of an embedding center or microtome and they are all thumbs! As far as a manager, I myself would rather have someone who has experience with histology over seeing my work. Just once again the lack of respect of having the HT/HTL behind your name. ________________________________ From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Thursday, August 25, 2011 10:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley; Podawiltz, Thomas Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? 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From talulahgosh <@t> gmail.com Thu Aug 25 16:16:26 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Aug 25 16:16:31 2011 Subject: [Histonet] MSDS binders In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238DE3B4177@MERCERMAIL.MercerU.local> References: <1314298694.6311.YahooMailNeo@web161608.mail.bf1.yahoo.com> <41F4AA9C-5271-45BA-8326-1516FF0D1017@yahoo.com> <4E569F82.6060003@pathology.washington.edu> <9BF995BC0E47744E9673A41486E24EE238DE3B4177@MERCERMAIL.MercerU.local> Message-ID: I would say if your computer system is down, then your electricity is probably out so I would hightail it out of there. Or ask yourself, why am I working with hazardous chemicals in the dark? Our EHS allows digital MSDS as well, but they say you could always call them if necessary. So maybe that's also what you would do if you computer system was down. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Thu, Aug 25, 2011 at 3:40 PM, Shirley A. Powell wrote: > Our Fire Marshalls even require we have a big red sign pointing to where > the hard copies are located. They don't need to hunt for it in case of an > event. > Shirley > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [ > Ronald.Houston@nationwidechildrens.org] > Sent: Thursday, August 25, 2011 3:31 PM > To: 'Victor Tobias'; William > Cc: Histonet@Lists. Edu > Subject: RE: [Histonet] MSDS binders > > There must be some way for the Fire Department to be able to access MSDS. > As Victor rightly points out, if your computer system is down, that option > no longer exists. It is not only prudent, it is mandatory for the Fire > Service to be able to look up any chemical within your facility. > > If you cannot satisfy your local fire daprtment, they will close you down > quicker than CAP or CLIA ever could. > > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias > Sent: Thursday, August 25, 2011 3:16 PM > To: William > Cc: Histonet@Lists. Edu > Subject: Re: [Histonet] MSDS binders > > In practicality you would turn to your online version first. What happens > if the computer system/network is down. Seems like it would be prudent to > have a hard copy available. > > Victor > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use of the > intended recipients. If you are not the intended recipient, or if the > message has been addressed to you in error, do not read, disclose, > reproduce, distribute, disseminate or otherwise use this transmission. > Instead, please notify the sender by reply e-mail, and then destroy all > copies of the message and any attachments. > > > On 8/25/2011 12:08 PM, William wrote: > > Not certain about CLIA, but CAP only requires immediate available access > to the MSDS's by any personal using chemicals. I interpret that as digital > copies ok, as long as everyone has access. I have passed three inspections > with digital MSDS only. > > > > Will Chappell > > > > Sent from my iPhone > > > > On Aug 25, 2011, at 11:58 AM, Jill Cox wrote: > > > >> Hello Histonetters, > >> > >> Does anyone know if there is a rule as to MSDS binders having to be in > yellow and black bold lettering? Do we even still need to have hard copy if > we have access to msds.com on desk top? I am in Ca and will be CLIA > licensed.. Thank you in advance!! > >> > >> Jill Cox, HT ASCP > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Thu Aug 25 16:18:49 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Aug 25 16:18:55 2011 Subject: [Histonet] MSDS binders In-Reply-To: References: <1314298694.6311.YahooMailNeo@web161608.mail.bf1.yahoo.com> <41F4AA9C-5271-45BA-8326-1516FF0D1017@yahoo.com> <4E569F82.6060003@pathology.washington.edu> <9BF995BC0E47744E9673A41486E24EE238DE3B4177@MERCERMAIL.MercerU.local> Message-ID: Actually now that I think about it, it would be faster to have a digital system, as you could search the MSDS file with the find option to get exactly what you needed. Also the most updated copies would be online always and you could google for them. And you would haven't to remember the actual chemical name for things like TESPA. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Thu, Aug 25, 2011 at 5:16 PM, Emily Sours wrote: > I would say if your computer system is down, then your electricity is > probably out so I would hightail it out of there. Or ask yourself, why am I > working with hazardous chemicals in the dark? > Our EHS allows digital MSDS as well, but they say you could always call > them if necessary. So maybe that's also what you would do if you computer > system was down. > > Emily > > A great book should leave you with many experiences, and slightly > exhausted. You should live several lives while reading it. > -William Styron > > > > On Thu, Aug 25, 2011 at 3:40 PM, Shirley A. Powell wrote: > >> Our Fire Marshalls even require we have a big red sign pointing to where >> the hard copies are located. They don't need to hunt for it in case of an >> event. >> Shirley >> >> >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu [ >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [ >> Ronald.Houston@nationwidechildrens.org] >> Sent: Thursday, August 25, 2011 3:31 PM >> To: 'Victor Tobias'; William >> Cc: Histonet@Lists. Edu >> Subject: RE: [Histonet] MSDS binders >> >> There must be some way for the Fire Department to be able to access MSDS. >> As Victor rightly points out, if your computer system is down, that option >> no longer exists. It is not only prudent, it is mandatory for the Fire >> Service to be able to look up any chemical within your facility. >> >> If you cannot satisfy your local fire daprtment, they will close you down >> quicker than CAP or CLIA ever could. >> >> >> Ronnie Houston >> Anatomic Pathology Manager >> Nationwide Children's Hospital >> Columbus OH 43205 >> (614) 722 5450 >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias >> Sent: Thursday, August 25, 2011 3:16 PM >> To: William >> Cc: Histonet@Lists. Edu >> Subject: Re: [Histonet] MSDS binders >> >> In practicality you would turn to your online version first. What happens >> if the computer system/network is down. Seems like it would be prudent to >> have a hard copy available. >> >> Victor >> >> Victor Tobias HT(ASCP) >> Clinical Applications Analyst >> University of Washington Medical Center >> Dept of Pathology Room BB220 >> 1959 NE Pacific >> Seattle, WA 98195 >> victor@pathology.washington.edu >> 206-744-2735 >> 206-744-8240 Fax >> ================================================= >> Privileged, confidential or patient identifiable information may be >> contained in this message. This information is meant only for the use of the >> intended recipients. If you are not the intended recipient, or if the >> message has been addressed to you in error, do not read, disclose, >> reproduce, distribute, disseminate or otherwise use this transmission. >> Instead, please notify the sender by reply e-mail, and then destroy all >> copies of the message and any attachments. >> >> >> On 8/25/2011 12:08 PM, William wrote: >> > Not certain about CLIA, but CAP only requires immediate available access >> to the MSDS's by any personal using chemicals. I interpret that as digital >> copies ok, as long as everyone has access. I have passed three inspections >> with digital MSDS only. >> > >> > Will Chappell >> > >> > Sent from my iPhone >> > >> > On Aug 25, 2011, at 11:58 AM, Jill Cox wrote: >> > >> >> Hello Histonetters, >> >> >> >> Does anyone know if there is a rule as to MSDS binders having to be in >> yellow and black bold lettering? Do we even still need to have hard copy if >> we have access to msds.com on desk top? I am in Ca and will be CLIA >> licensed.. Thank you in advance!! >> >> >> >> Jill Cox, HT ASCP >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> ----------------------------------------- Confidentiality Notice: >> The following mail message, including any attachments, is for the >> sole use of the intended recipient(s) and may contain confidential >> and privileged information. The recipient is responsible to >> maintain the confidentiality of this information and to use the >> information only for authorized purposes. If you are not the >> intended recipient (or authorized to receive information for the >> intended recipient), you are hereby notified that any review, use, >> disclosure, distribution, copying, printing, or action taken in >> reliance on the contents of this e-mail is strictly prohibited. If >> you have received this communication in error, please notify us >> immediately by reply e-mail and destroy all copies of the original >> message. Thank you. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From raestask <@t> grics.net Thu Aug 25 16:53:36 2011 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Thu Aug 25 16:53:44 2011 Subject: [Histonet] AMPHYL In-Reply-To: References: <7DA79EBDBD92BF408EF392413737878D394AB99B4D@NADCWPMSGCMS01.hca.corpad.net> Message-ID: <2F4286AE92E64EBF9FB3463CA8A36037@your4105e587b6> Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Aug 25 17:17:21 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Aug 25 17:17:25 2011 Subject: [Histonet] AMPHYL In-Reply-To: <2F4286AE92E64EBF9FB3463CA8A36037@your4105e587b6> References: <7DA79EBDBD92BF408EF392413737878D394AB99B4D@NADCWPMSGCMS01.hca.corpad.net> <2F4286AE92E64EBF9FB3463CA8A36037@your4105e587b6> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B03A@EXCHANGE3.huntingtonhospital.com> We get ours from Lab Safety Supply. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz Sent: Thursday, August 25, 2011 2:54 PM To: 'Sebree Linda A'; Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Tokugawa <@t> kp.org Thu Aug 25 19:08:01 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Thu Aug 25 19:08:16 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 08/25/2011 and will not return until 08/29/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, Barbara at 8-421-5033, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, general histology lab 8-421- 5408 or Wanda at 8-421-5426. From jnocito <@t> satx.rr.com Thu Aug 25 19:07:50 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Aug 25 19:09:05 2011 Subject: [Histonet] RE: New CAP question In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37120@EXCHANGESB> References: <24A4826E8EF0964D86BC5317306F58A55FC2870DCB@mmc-mail.ad.mhsil.com> <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37120@EXCHANGESB> Message-ID: <48E7D7D936B1453EA23D4D1908CF2235@JoePC> Greetings all, I contacted CAP just before my on-site inspection last year. I was told that all validation studies had to be on file until the antibody/ special stain/ or equipment was no longer used. Sad to say. Now, I understand for 2012, CAP has added another "lab specific" checklist. My understanding is that some of the General Checklist questions will now be department specific. It's like a subset of the General Checklist. I was told by a co-worker, I haven't seen anything in print yet, but come on people. A General department checklist from the General checklist. Then why have a General Checklist? Let's keep adding checklists because we can? This is what happens when a monopoly is formed. I'm going to start my own inspection company. I'm going to start from the toes and work my way up. JTT ----- Original Message ----- From: "Carol Bryant" To: "'Vickroy, Jim'" ; Sent: Thursday, August 25, 2011 11:09 AM Subject: [Histonet] RE: New CAP question Please respond to all. I would like the information also. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JBAR <@t> ANA.AU.DK Fri Aug 26 02:08:29 2011 From: JBAR <@t> ANA.AU.DK (Jette Barlach) Date: Fri Aug 26 02:05:20 2011 Subject: [Histonet] heavy duty microtome Message-ID: <6841742E8971D4438A935427959D30132055C95AA0@SIF.svf.au.dk> Does anyone have either a used Leica Polycut E or SM 2500 for sale. For the present we are using a very old (50 years) Reichert-Jung heavy duty microtome and looking for a replacement. If you can help us please contact: Jette Barlach Research unit for Rheumatology an Bone biology Dept. of Rheumatology University hospital of Aarhus N?rrebrogade 44 8000 Aarhus C Denmark jbar@ana.au.dk +45 89 42 29 92 From k84as <@t> yahoo.com Fri Aug 26 05:29:24 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Aug 26 05:29:27 2011 Subject: [Histonet] (no subject) Message-ID: <1314354564.91036.YahooMailMobile@web112616.mail.gq1.yahoo.com> http://hekkles.com/vffws.htm From NHeath <@t> Lifespan.org Fri Aug 26 06:10:19 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Fri Aug 26 06:10:28 2011 Subject: [Histonet] RE: Embedding process improvementandcompetencyassessment In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93951922AD@chimsx08.CHI.catholichealth.net> Message-ID: <130E8991F210424096EFC6F42EA33B2408043557@LSCOEXCH1.lsmaster.lifespan.org> "If people cheated..... Then people cheated. There will always be that element. To discard that requirement (the practical) because of the cheat factor was a silly (remember, this is my rant....not yours) excuse that I have heard from some people who were part of the decision. What was lost was the incredable effort that was needed to do that practical and the experience of being able to produce "registry" quality slides. That is not cheating themselves, but robbing every pathologist and patient that followed until that person started to produce quality work." I could not agree more with what Bill O'Donnell wrote!! Thank you Bill. I have students in the clinical portion of there histo training and though I am not former military my students sure do think I am!! I strive for perfection from the time they section in their first piece of tissue till their last stained slide. Nancy Heath, HT(ASCP) -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: Thursday, August 25, 2011 4:48 PM To: Podawiltz, Thomas; Heath, Nancy L.; Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvementandcompetencyassessment Tom, (and all those following this thread) And we got that ;) perfection. (Warning, long rant ahead... Rene's might have been longer, but he spread it out over a number of posts) (Hi Rene, I enjoyed your rant.) My biggest barrier in assimilating into the "civilian" workforce was that perfection wasn't required or even at times expected. I still can't get my head around that. Because of what was expected of me by my unit, 30 years later, my embedding is tight w nice, neat rows, cutting is neat and aligned and staining is crisp. (However, I will not win any speed contest) My point in this is not to blow my horn. Most of you out there turn out the same or better quality. My point is this, when supervisors and peers stop striving for perfection then there will always be a need for skills assessments and re-training. There will be little or no perfection. Over the last thirty years of training and supervising, I have always tried to instill the idea that we are dealing with a person's tissue. Wrapped up in that is a lot of anxiety and stress that the patient is having. The first thing a new trainee or tech had to do when they came to me (in the civilian world)was spend a week shadowing phlebotomy so that they got the chance to see some of the faces behind the samples. But in the last ten years or so, there has been too much emphasis on speed as a standard of performance, and in general, there has been a growing attitude that perfection isn't possible. (many factors to blame, I suppose) I once worked for a very demanding pathologist and because of his expectations, the whole crew put out near perfection. Pathologists that are "OK" with what they get, so long as they can make a diagnosis, are a huge part of that problem. But as supervisors, we only get what we expect of people and strive for and example ourselves. (The rest of the rant is anecdotal and not very interesting, but since I took the time to write it, here it is...) This was also the period (Navy)when I had to do that monster practical for the HT. Back then, it was not 7 or 8 slides, but many more with a large number of special stains as well. I knew if it passed the guys in our lab, it should be no problem passing the practical. The guy who took the test at the same time I did, picked his tissues on Wednesday, embedded them on Thursday cut cut and stained them on Saturday and mailed them on Monday. He had no concern at all that his work wouldn't be good enough. He passed the practical with a very respectable percentile. I think that helps to bolster the idea that an expectation that is demanded and fostered is one that can be confidently met. If people cheated..... Then people cheated. There will always be that element. To discard that requirement (the practical) because of the cheat factor was a silly (remember, this is my rant....not yours) excuse that I have heard from some people who were part of the decision. What was lost was the incredable effort that was needed to do that practical and the experience of being able to produce "registry" quality slides. That is not cheating themselves, but robbing every pathologist and patient that followed until that person started to produce quality work. (Rany over, thanks to those who hung out til the end.... I hope it was worth it) I hope every one has a great weekend, as I am off on Friday and will be having a great weekend myself. Shalom - Bill -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Thursday, August 25, 2011 1:21 PM To: O'Donnell, Bill; Heath, Nancy L.; Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvementandcompetencyassessment Man did that bring back some memories. Bill and I worked together in the same Navy lab and he is correct if a block was embedded wrong you would get it in either the back of the head or between the shoulders. They stopped throwing them at me the day I throw the block back at the microtomist and hit him in the head. We were a tough crew that saw a lot, did a lot, partied together a lot, but never lost the fact that we were there for patient care and treated all the specimens and bodies as if they came from a love one. When it came time for Bill and I to become the trainers we were brutal in how to embed, section and all other aspects of histology, I mean we only wanted perfection. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Thursday, August 25, 2011 1:57 PM To: Heath, Nancy L.; Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvementand competencyassessment Just to throw a somewhat funny situation into the mix. I learned my embedding skills in the Navy. The basic method for learning all tasks was 1. explain it, 2. demonstrate it, 3. do it. Each microtomist was responsible for taking a good look at the block before cutting it. If the embedding was not spot-on, the block was always returned for reembedding. The method of return was to throw it at the back of my head. They rarely missed. Very quickly, I got tired of being hit in the head and my embedding improved. Can't do that kind of stuff now, even in the military. Who knew that histology used to be a contact sport? We've gotten sooooo soft! As to the other situation, as a histology supervisor, I was over cytology in one of the labs I worked at. I doubt that I was much of a manager to them as I knew next to nothing about their work. However, I took the time to learn some aspects, and then just pretty much left them alone. (It was a mutual respect, they pretty much left me alone too.) They did a fine job of making me look good. I trusted them, and they didn't do anything to betray that trust. Because of that, I would simply sign-off on the occasional request. Evals were pretty easy as I simply interviewed the pathologists, checked attendance and moved on. That being said, I wouldn't want to have to do it again. They deserved better, but we made it work, since it wasn't going to change. Have a great day! William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 Check out my podcast at DeaconCast.Net SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Thursday, August 25, 2011 11:39 AM To: Jennifer MacDonald Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio,Shelley Subject: RE: [Histonet] RE: Embedding process improvementand competencyassessment Regardless of wether there were 8 blocks or eighteen blocks taking the practical taught me to be precise with all of the hands on aspects of Histology. Shame on the older techs from the "practical days" of not keeping on top of their game with embedding. My comment was geared more towards the newbies coming out of histo schools who can pass the exam with flying colors but sit them in front of an embedding center or microtome and they are all thumbs! As far as a manager, I myself would rather have someone who has experience with histology over seeing my work. Just once again the lack of respect of having the HT/HTL behind your name. ________________________________ From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Thursday, August 25, 2011 10:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley; Podawiltz, Thomas Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. "Heath, Nancy L." Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2011 07:11 AM To "D'Attilio, Shelley" , "Podawiltz, Thomas" , "Histonet Listserv (E-mail)" cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the "Slide Quality Review Form" that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I have purchased quite a few resources over the years from NSH, and even attended the NSH annual meeting a few years ago when it was in Phoenix. I will reconsider my decision to drop my membership. For those on the list, here is Tom's response to my question: Hi Shelley, I would suggest you join NSH, they have all kinds of reference material for this type of work. Please tell me you are off the bench, you have a lot to monitor and if you are working the bench on top of your management duties my prayers go out to you. Embedding: 1. Proper size of mold in relation to specimen size. 2. Proper orientation of tissue, example 5 skin biopsies, dermis must face the same direction, and be at an angle to the blade so when you cut the section cuts smoothly and doesn't roll up. 3. Multiple pieces all on the same plane. If one piece is deeper than the others you must re-embed, or you will cut through the other pieces before you reach it. 4. Make sure that the embedding unit is wipe down between each case as are the forceps, this will avoid tissue floaters. 5. Never open more than one cassette at a time. 6. Verify that the piece count on the work sheet matches what is in the cassette when it is opened. 7. Never hound the staff about speed, accuracy is more important, speed comes with experience. If its embedded wrong, it will be cut wrong and this will effect diagnosis. 8. What do you do for QA on the slides?I have a work sheet that the Pathologist fills out each day about the slides, which is the end product of embedding. I hope my tips help you and feel free to contact me if you need anything. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ************************************************************************ ****************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ************************************************************************ ****************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Montina.VanMeter <@t> pbrc.edu Fri Aug 26 07:19:57 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri Aug 26 07:20:18 2011 Subject: [Histonet] heavy duty microtome In-Reply-To: <6841742E8971D4438A935427959D30132055C95AA0@SIF.svf.au.dk> References: <6841742E8971D4438A935427959D30132055C95AA0@SIF.svf.au.dk> Message-ID: Contact Leica to see if they have any demo models for sale. Sent from my iPhone On Aug 26, 2011, at 2:10 AM, "Jette Barlach" wrote: > Does anyone have either a used Leica Polycut E or SM 2500 for sale. > For the present we are using a very old (50 years) Reichert-Jung heavy duty microtome and looking for a replacement. > > If you can help us please contact: > > Jette Barlach > Research unit for Rheumatology an Bone biology > Dept. of Rheumatology > University hospital of Aarhus > N?rrebrogade 44 > 8000 Aarhus C > Denmark > jbar@ana.au.dk > +45 89 42 29 92 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Fri Aug 26 08:08:01 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Aug 26 08:08:08 2011 Subject: [Histonet] heavy duty microtome In-Reply-To: References: <6841742E8971D4438A935427959D30132055C95AA0@SIF.svf.au.dk> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3203098FA8@Mail2Node2.ad.uams.edu> I have used the Leica RM2255 and RM 2155s for paraffin and non-decalcified bone in MMA and they are work horses. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Friday, August 26, 2011 7:20 AM To: Jette Barlach Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] heavy duty microtome Contact Leica to see if they have any demo models for sale. Sent from my iPhone On Aug 26, 2011, at 2:10 AM, "Jette Barlach" wrote: > Does anyone have either a used Leica Polycut E or SM 2500 for sale. > For the present we are using a very old (50 years) Reichert-Jung heavy duty microtome and looking for a replacement. > > If you can help us please contact: > > Jette Barlach > Research unit for Rheumatology an Bone biology Dept. of Rheumatology > University hospital of Aarhus N?rrebrogade 44 8000 Aarhus C Denmark > jbar@ana.au.dk > +45 89 42 29 92 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From mucram11 <@t> comcast.net Fri Aug 26 08:39:16 2011 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri Aug 26 08:39:22 2011 Subject: [Histonet] AMPHYL In-Reply-To: <2F4286AE92E64EBF9FB3463CA8A36037@your4105e587b6> Message-ID: <769717426.529941.1314365956569.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We can't find Lysol IC here either so any supplier would help and to keep costs down a store would be better than a scientific supplier it possible.? Please id anyone knows let us all know as it is not easy to find. Pam Marcum ----- Original Message ----- From: "Rae Staskiewicz" To: "Sebree Linda A" , "Barbara Crill" , histonet@lists.utsouthwestern.edu Sent: Thursday, August 25, 2011 4:53:36 PM Subject: RE: [Histonet] AMPHYL Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? ?Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bouchalr <@t> wvuhealthcare.com Fri Aug 26 08:44:58 2011 From: bouchalr <@t> wvuhealthcare.com (Bouchal, Rena L) Date: Fri Aug 26 08:43:54 2011 Subject: [Histonet] AMPHYL In-Reply-To: <769717426.529941.1314365956569.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <2F4286AE92E64EBF9FB3463CA8A36037@your4105e587b6> <769717426.529941.1314365956569.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: After Amphyl was discontinued, we switched to Steris Lph Se. Here are the comparisons of the two: Both are germicidal, fungicidal, and virucidal; both contain active ingredients 0-phenylophenol (amphyl at 2.80%and Steris at 7.7%) instead of the other active ingredient in amphyl (0-benzyl-p-chlorophenol at 7.2%) Steris contains p-tertiary-amphylphenol at 7.6%. Amphyl is diluted 40cc/1gal for a 1:100 ratio and Steris is diluted 30cc/1gal for 1:100 ratio. The order number for the Steris LpH Se is 6466-08. Hope this helps. Please note that my email address as of Jan 3, 2011 is bouchalr@wvuhealthcare.com . Please make the appropriate changes in your address book. Rena Bouchal, M.S. Anatomic Pathology Manager West Virginia University Hospitals 304-293-7765 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Friday, August 26, 2011 9:39 AM To: Rae Staskiewicz Cc: histonet@lists.utsouthwestern.edu; Barbara Crill; Sebree Linda A Subject: Re: [Histonet] AMPHYL We can't find Lysol IC here either so any supplier would help and to keep costs down a store would be better than a scientific supplier it possible.? Please id anyone knows let us all know as it is not easy to find. Pam Marcum ----- Original Message ----- From: "Rae Staskiewicz" To: "Sebree Linda A" , "Barbara Crill" , histonet@lists.utsouthwestern.edu Sent: Thursday, August 25, 2011 4:53:36 PM Subject: RE: [Histonet] AMPHYL Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? ?Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From twheelock <@t> mclean.harvard.edu Fri Aug 26 08:54:27 2011 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Aug 26 08:54:31 2011 Subject: [Histonet] Embedding Center Questions Message-ID: <4E57A593.90208@mclean.harvard.edu> Hi All: My 23 year old Shandon Embedding Center may be ready to give up the ghost. It has had two repairs of the coolant system for the freezing plate over the last year. Now a thermo-resistor has burnt out, so that the wax reservoir with no long melt the wax. Does anyone know of a company or companies that lease/rent histology equipment? Are there companies who sell used equipment? Also, what do people recommend as far as a new embedding center, in case we can afford it? I am in the Boston area. In the meantime, maybe I can jury-rig something, since it is only the wax reservoir that is down. The cassette holding tank, hot plate, and freezing plate are OK for the moment. Maybe I can melt a few jars of wax, and use a transfer pipet to fill the molds? Any ideas? Thanks, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 From SDattili <@t> stormontvail.org Fri Aug 26 08:57:35 2011 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Fri Aug 26 08:58:33 2011 Subject: [Histonet] Embedding competency and med techs running a Histo lab Message-ID: Hi all, Thanks so much for the advice I received following my request for help on our embedding competency improvement project. I enjoyed the lively discussion that followed about non-histotechs managing a Histology lab and I appreciated all the kind comments and words of encouragement both on and off the listserv board. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From LSebree <@t> uwhealth.org Fri Aug 26 09:02:24 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Aug 26 09:02:57 2011 Subject: [Histonet] AMPHYL In-Reply-To: <769717426.529941.1314365956569.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <2F4286AE92E64EBF9FB3463CA8A36037@your4105e587b6> <769717426.529941.1314365956569.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: We've been getting our Lysol IC from Cardinal Health but in the past we've had to go through Amazon.com Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ________________________________ From: Pam Marcum [mailto:mucram11@comcast.net] Sent: Friday, August 26, 2011 8:39 AM To: Rae Staskiewicz Cc: Sebree Linda A; Barbara Crill; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] AMPHYL We can't find Lysol IC here either so any supplier would help and to keep costs down a store would be better than a scientific supplier it possible. Please id anyone knows let us all know as it is not easy to find. Pam Marcum ________________________________ From: "Rae Staskiewicz" To: "Sebree Linda A" , "Barbara Crill" , histonet@lists.utsouthwestern.edu Sent: Thursday, August 25, 2011 4:53:36 PM Subject: RE: [Histonet] AMPHYL Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Aug 26 09:10:32 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Aug 26 09:10:49 2011 Subject: [Histonet] Embedding Center Questions In-Reply-To: <4E57A593.90208@mclean.harvard.edu> References: <4E57A593.90208@mclean.harvard.edu> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F53E0C@SMCMAIL01.somerset-healthcare.com> A couple of places that come to mind are Belair (800-783-9424), Medical Equipment Source (www.medequipsource.com), and Mayflower (800-287-9801). I know that they sell used equipment, you could inquire about a rental. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, August 26, 2011 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Center Questions Hi All: My 23 year old Shandon Embedding Center may be ready to give up the ghost. It has had two repairs of the coolant system for the freezing plate over the last year. Now a thermo-resistor has burnt out, so that the wax reservoir with no long melt the wax. Does anyone know of a company or companies that lease/rent histology equipment? Are there companies who sell used equipment? Also, what do people recommend as far as a new embedding center, in case we can afford it? I am in the Boston area. In the meantime, maybe I can jury-rig something, since it is only the wax reservoir that is down. The cassette holding tank, hot plate, and freezing plate are OK for the moment. Maybe I can melt a few jars of wax, and use a transfer pipet to fill the molds? Any ideas? Thanks, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From tkngflght <@t> yahoo.com Fri Aug 26 09:17:08 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Aug 26 09:17:15 2011 Subject: [Histonet] Jill's MSDS question: a tech's perspective Message-ID: <1314368228.65620.YahooMailNeo@web39410.mail.mud.yahoo.com> Hi Jill-- Congrats on the job! ? The MSDS in paper vs. eCopy?copy will come down to the opinion of your inspector.? Like Wil says, he's passed before...and I've passed before but only because we had both eCopies and hard copies.? Most of the larger for-profit and major health system administrations?require both by SOP regardless, for liability reasons usually but please read on! ? Better yet, think of the practical uses and real-world applications of?this?rather than the 'do it because I have to' ones: ? If you or one of yours gets splashed with something--how do you want the next response to go?? If you only have eCopies and the power is down, or you?are having computer issues, or the PC is slow, or?the printer on the network isn't responding, then what? If it's your eyes or your skin that's about to be irreversably burnt, wouldn't you want that information available in a TOTALLY reliable manner and a copy available to?grab,?follow?AND send to the emergency room with you--RIGHT NOW?? Then add to it the liability for your company should this be the case...and we all know when you most need your PC to work is when it doesn't. ? If I'm having trouble making a decision often exaggerating the potential circumstances makes the answer far easier to come to. ? If I am working in a lab I want hard copies alphabetized by both the mfc. chemical name and the common name we use for it in the lab with an index?with the binders (Print the covers black on yellow paper and insert these in clear view binders)?on the benches in the space where we work.? Not on a computer or off in the transcription area.? It may not be required by your inspector or by your facility, but is that really why we have these in our labs in the first place--because we're required to do so?? ? My four cents.... ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc - Staffing the AP Lab one GREAT tech at a time. 281.852.9457 admin@fullstaff.org From ratliffjack <@t> hotmail.com Fri Aug 26 09:24:46 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Aug 26 09:24:50 2011 Subject: [Histonet] heavy duty microtome In-Reply-To: <6841742E8971D4438A935427959D30132055C95AA0@SIF.svf.au.dk> References: <6841742E8971D4438A935427959D30132055C95AA0@SIF.svf.au.dk> Message-ID: I know of two possible options for exactly what you seek. Please contact me directly so that we may point you in the right direction and discuss with you the details. Best Regards, Jack PS I have heard a rumor that these units will no longer be for sale by Leica and no replacements will be available in the future. Additionally, I very seriously doubt that they have a demo unit for sale as they typically manufacture these units upon approved purchase order. Jack L Ratliff Senior Histologist, BioMimetic Therapeutics, Inc. Chair, Hard Tissue Committee - National Society for Histotechnology > From: JBAR@ANA.AU.DK > To: histonet@lists.utsouthwestern.edu > Date: Fri, 26 Aug 2011 09:08:29 +0200 > Subject: [Histonet] heavy duty microtome > > Does anyone have either a used Leica Polycut E or SM 2500 for sale. > For the present we are using a very old (50 years) Reichert-Jung heavy duty microtome and looking for a replacement. > > If you can help us please contact: > > Jette Barlach > Research unit for Rheumatology an Bone biology > Dept. of Rheumatology > University hospital of Aarhus > N?rrebrogade 44 > 8000 Aarhus C > Denmark > jbar@ana.au.dk > +45 89 42 29 92 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Aug 26 09:33:18 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Aug 26 09:33:36 2011 Subject: [Histonet] AMPHYL In-Reply-To: <769717426.529941.1314365956569.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <2F4286AE92E64EBF9FB3463CA8A36037@your4105e587b6>, <769717426.529941.1314365956569.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: http://www.labsafety.com/lysol-ic-quaternary-cleaner-disinfectant_24530427/ Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum [mucram11@comcast.net] Sent: Friday, August 26, 2011 9:39 AM To: Rae Staskiewicz Cc: histonet@lists.utsouthwestern.edu; Barbara Crill; Sebree Linda A Subject: Re: [Histonet] AMPHYL We can't find Lysol IC here either so any supplier would help and to keep costs down a store would be better than a scientific supplier it possible. Please id anyone knows let us all know as it is not easy to find. Pam Marcum ----- Original Message ----- From: "Rae Staskiewicz" To: "Sebree Linda A" , "Barbara Crill" , histonet@lists.utsouthwestern.edu Sent: Thursday, August 25, 2011 4:53:36 PM Subject: RE: [Histonet] AMPHYL Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gina.Rodriguez <@t> leica-microsystems.com Fri Aug 26 10:00:52 2011 From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com) Date: Fri Aug 26 10:01:02 2011 Subject: [Histonet] AUTO: Gina Rodriguez is out of the office. (returning 09/06/2011) Message-ID: I am out of the office until 09/06/2011. I will respond to your message when I return. Note: This is an automated response to your message "Histonet Digest, Vol 93, Issue 35" sent on 8/26/2011 8:45:07 AM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From relia1 <@t> earthlink.net Fri Aug 26 10:07:21 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Aug 26 10:07:14 2011 Subject: [Histonet] OT: Hurricane Irene Message-ID: Hi Histonetters! Are you in the projected path of Irene? I'm a long time florida resident who has weathered hurricanes&false alarms in both central Florida&the panhandle. It's not the hurricane it's the aftermath... No power which means no gasoline no atms no A.C. no lights, no water. At least fill your car with gas have cash on hand&fill your bathtub with water That way you are prepared and have not spent alot of money on stuff you wouldn't use on the OFF chance that it doesn't affect you. Be Safe!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From POWELL_SA <@t> mercer.edu Fri Aug 26 10:07:19 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Aug 26 10:07:23 2011 Subject: [Histonet] AMPHYL In-Reply-To: <769717426.529941.1314365956569.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <2F4286AE92E64EBF9FB3463CA8A36037@your4105e587b6> <769717426.529941.1314365956569.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE438B6E@MERCERMAIL.MercerU.local> Google Lysol IC and you will get plenty of suppliers. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Friday, August 26, 2011 9:39 AM To: Rae Staskiewicz Cc: histonet@lists.utsouthwestern.edu; Barbara Crill; Sebree Linda A Subject: Re: [Histonet] AMPHYL We can't find Lysol IC here either so any supplier would help and to keep costs down a store would be better than a scientific supplier it possible.? Please id anyone knows let us all know as it is not easy to find. Pam Marcum ----- Original Message ----- From: "Rae Staskiewicz" To: "Sebree Linda A" , "Barbara Crill" , histonet@lists.utsouthwestern.edu Sent: Thursday, August 25, 2011 4:53:36 PM Subject: RE: [Histonet] AMPHYL Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? ?Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Fri Aug 26 10:21:49 2011 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Fri Aug 26 10:21:52 2011 Subject: [Histonet] Jill's MSDS question: a tech's perspective In-Reply-To: <1314368228.65620.YahooMailNeo@web39410.mail.mud.yahoo.com> References: <1314368228.65620.YahooMailNeo@web39410.mail.mud.yahoo.com> Message-ID: <1314372109.6931.YahooMailNeo@web161605.mail.bf1.yahoo.com> Hi Cheryl, Thanks! I do have hard copies here, my main question was about the binder color. I ended up ordering a MSDS binder from LSS yesterday. I just wanted all manuals to match, lol. I haven't had hard copy in years, everywhere I have been has only had online and saved to desktop. We did pass all inspections with online only. It's great to hear from you! Jill Cox, HT ASCP ? ? From: Cheryl To: "histonet@lists.utsouthwestern.edu" Sent: Friday, August 26, 2011 7:17 AM Subject: [Histonet] Jill's MSDS question: a tech's perspective Hi Jill-- Congrats on the job! ? The MSDS in paper vs. eCopy?copy will come down to the opinion of your inspector.? Like Wil says, he's passed before...and I've passed before but only because we had both eCopies and hard copies.? Most of the larger for-profit and major health system administrations?require both by SOP regardless, for liability reasons usually but please read on! ? Better yet, think of the practical uses and real-world applications of?this?rather than the 'do it because I have to' ones: ? If you or one of yours gets splashed with something--how do you want the next response to go?? If you only have eCopies and the power is down, or you?are having computer issues, or the PC is slow, or?the printer on the network isn't responding, then what? If it's your eyes or your skin that's about to be irreversably burnt, wouldn't you want that information available in a TOTALLY reliable manner and a copy available to?grab,?follow?AND send to the emergency room with you--RIGHT NOW?? Then add to it the liability for your company should this be the case...and we all know when you most need your PC to work is when it doesn't. ? If I'm having trouble making a decision often exaggerating the potential circumstances makes the answer far easier to come to. ? If I am working in a lab I want hard copies alphabetized by both the mfc. chemical name and the common name we use for it in the lab with an index?with the binders (Print the covers black on yellow paper and insert these in clear view binders)?on the benches in the space where we work.? Not on a computer or off in the transcription area.? It may not be required by your inspector or by your facility, but is that really why we have these in our labs in the first place--because we're required to do so?? ? My four cents.... ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc - Staffing the AP Lab one GREAT tech at a time. 281.852.9457 admin@fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Aug 26 10:29:20 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Aug 26 10:29:25 2011 Subject: [Histonet] Jill's MSDS question: a tech's perspective In-Reply-To: <1314368228.65620.YahooMailNeo@web39410.mail.mud.yahoo.com> References: <1314368228.65620.YahooMailNeo@web39410.mail.mud.yahoo.com> Message-ID: I would respond to this by saying, if I were working with something that dangerous, I would know ahead of time what to do with it. I'm not going to spend any amount of time looking up an MSDS if something is on me RIGHT NOW, digital or not. Which is why I think it makes sense to have digital copies, because you're going to know how to handle hazardous stuff and digital copies save space and time. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Aug 26, 2011 at 10:17 AM, Cheryl wrote: > Hi Jill-- Congrats on the job! > > The MSDS in paper vs. eCopy copy will come down to the opinion of your > inspector. Like Wil says, he's passed before...and I've passed before but > only because we had both eCopies and hard copies. Most of the larger > for-profit and major health system administrations require both by SOP > regardless, for liability reasons usually but please read on! > > Better yet, think of the practical uses and real-world applications > of this rather than the 'do it because I have to' ones: > > If you or one of yours gets splashed with something--how do you want the > next response to go? If you only have eCopies and the power is down, or > you are having computer issues, or the PC is slow, or the printer on the > network isn't responding, then what? If it's your eyes or your skin that's > about to be irreversably burnt, wouldn't you want that information available > in a TOTALLY reliable manner and a copy available to grab, follow AND send > to the emergency room with you--RIGHT NOW? Then add to it the liability for > your company should this be the case...and we all know when you most need > your PC to work is when it doesn't. > > If I'm having trouble making a decision often exaggerating the potential > circumstances makes the answer far easier to come to. > > If I am working in a lab I want hard copies alphabetized by both the mfc. > chemical name and the common name we use for it in the lab with an > index with the binders (Print the covers black on yellow paper and insert > these in clear view binders) on the benches in the space where we work. Not > on a computer or off in the transcription area. It may not be required by > your inspector or by your facility, but is that really why we have these in > our labs in the first place--because we're required to do so? > > My four cents.... > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc - Staffing the AP Lab one GREAT tech at a time. > 281.852.9457 > admin@fullstaff.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Fri Aug 26 10:36:15 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Aug 26 10:36:19 2011 Subject: [Histonet] Re: Embedding process etc. Message-ID: Bill O'Donnell observes: >>I once worked for a very demanding pathologist and because of his expectations, the whole crew put out near perfection. Pathologists that are "OK" with what they get, so long as they can make a diagnosis, are a huge part of that problem.<< I very much agree, though I've often been guilty as charged. Edwards Deming - whom of course American MBA's learn to ridicule - was very much concerned with constant feedback, and constant attention to fixing little problems before they turn into big problems. The histotechnologist who never looks at a slide and the pathologist who is afraid to complain about unsatisfactory work are two sides of the same problem. This month's Journal of Histotechnology has an article about quality assurance of GI biopsies that everyone concerned with this issue should read. I'll try to get review of it onto Histonet. Bob Richmond Samurai Pathologist Knoxville TN From Diane.Tokugawa <@t> kp.org Fri Aug 26 12:00:51 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Fri Aug 26 12:01:12 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 08/25/2011 and will not return until 08/29/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, Barbara at 8-421-5033, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, general histology lab 8-421- 5408 or Wanda at 8-421-5426. From joelleweaver <@t> hotmail.com Fri Aug 26 12:14:03 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Aug 26 12:14:12 2011 Subject: [Histonet] RE: Embedding process improvement and competencyassessment In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46106DC54A@hc-mailboxc1-n3.healthcare.uiowa.edu> References: <130E8991F210424096EFC6F42EA33B2408043363@LSCOEXCH1.lsmaster.lifespan.org>, , <493CAA64F203E14E8823737B9EE0E25F0900642E5F@EXCHMB01.isis.local>, <6DC87DEA9229894DB3A09F8B61717A46106DC54A@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: I recall being told that one reason for the practical discontinuation, was that there were not enough well qualified judges for the practical work available at the ASCP, mostly populated with MT trained people in these roles? ..not sure if that is true.I am not offering that this is valid for a reason, just that this was the information I was given when I questioned the wisdom of that decision. I do think that if you cheat, this will catch up with you,but unfortunately not immediately most of the time. Sadly I think it does serve to dilute the perception of, and respect for, those that had put forth the effort to acheive their best , and submit their best slides(closest to perfection) by their own efforts. I can see it might be hard to prove cheaters, given the geographic area and number of people involved, but anyone who signed off on someone's misrepresented work, well they have to live with themselves and whatever the potential results to patients, colleagues et al. Joelle Joelle Weaver MAOM, BA, (HTL) ASCP > From: joseph-galbraith@uiowa.edu > To: BDeBrosse-Serra@isisph.com; JMacDonald@mtsac.edu; NHeath@Lifespan.org > Date: Thu, 25 Aug 2011 18:07:26 +0000 > Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment > CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; SDattili@stormontvail.org > > How disgusting to hear about cheating. I recall that someone was supposed to sign off as a witness that the applicant had done the work themselves. I spent months acquiring tissue, processing, embedding, cutting and staining a set of blocks and slides and was rewarded with a high score for the effort. It was something I could be proud of. As I recall we had to submit 25 or so slides back then only some of which were graded and the grading was really strict (but did vary with the grader). > > Joe Galbraith HTL (and also MT by the way) > University of Iowa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra > Sent: Thursday, August 25, 2011 12:14 PM > To: 'Jennifer MacDonald'; Heath, Nancy L. > Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley > Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment > > I heard of a lot of cheating as well. People paid others to do the blocks and staining. How good does it do? In the end, these people are cheating themselves. Very sad! > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 1896 Rutherford Road > Carlsbad, CA 92008 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald > Sent: Thursday, August 25, 2011 7:58 AM > To: Heath, Nancy L. > Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu; D'Attilio, Shelley > Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment > > I fail to see the correlation of a non HT person supervising the Histology > lab and the lack of a practical exam for HT/HTL staff. One of the issues > that Shelley brought up was the staff lost or did not develop their > embedding skills. Submission of a practical exam is not proof of highly > developed embedding skills. For the HT exam there were 8 blocks that were > submitted (9 slides). I know of cases where the blocks were not even > embedded or cut by the applicant. > > > > > "Heath, Nancy L." > Sent by: histonet-bounces@lists.utsouthwestern.edu > 08/25/2011 07:11 AM > > To > "D'Attilio, Shelley" , "Podawiltz, Thomas" > , "Histonet Listserv (E-mail)" > > cc > > Subject > RE: [Histonet] RE: Embedding process improvement and competencyassessment > > > > > > > This is exactly why the powers that be should have NEVER gotten rid of > the practical portion of the HT/HTL board certification! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > D'Attilio, Shelley > Sent: Thursday, August 25, 2011 9:45 AM > To: Podawiltz, Thomas; Histonet Listserv (E-mail) > Subject: [Histonet] RE: Embedding process improvement and > competencyassessment > > Hi Tom, > Thank you for your kind words. I am off the bench almost completely. I > can work in the gross room in a pinch and my counting skills are > excellent, so I can always file slides and block if an emergency > arises:) I occasionally cover a bench in Chemistry as well, but my > staff is all pretty glad that I mostly stay in my office. > > Thanks so much for the embedding information. The main problem we are > tackling at the moment is tissue orientation. I have written a pretty > detailed embedding procedure that is being reviewed by the new histology > supervisor. Our plan is to refresh the training of everyone on staff in > conjunction with this procedure, then add specific embedding > competencies to our checklist. I will make sure that the procedure > incorporates the first 6 elements that you listed below. > > Currently we have a QA sheet that is given to the pathologist with each > batch of slides. Pathologists provide us with feedback on the slide > quality by filling out the form. Slides with sub-standard > quality--whether in orientation, cutting, staining, whatever--our > reviewed by every histotech in the lab with an aim to education and > improvement of performance. We have a form called the "Slide Quality > Review Form" that details the quality issue. Techs are directed to > review the slides and comment. Difficult cases or those where people > disagree are discussed in our department meetings. > > One of our difficulties over the years has been how the work was divided > between the histotechs. One histotech loved to embed and was very good > at it, so he did most of the embedding. He eventually moved to an > overnight shift, which resulted in him embedding even more than he was. > Consequently, other staff people either lost their skills or never fully > developed them. It was introduction of rapid processing that really > brought this issue to the forefront, since different people were > embedding at different times of the day. > > Unfortunately, I let my NSH membership lapse this year for budgetary > reasons. I have purchased quite a few resources over the years from > NSH, and even attended the NSH annual meeting a few years ago when it > was in Phoenix. I will reconsider my decision to drop my membership. > > For those on the list, here is Tom's response to my question: > > Hi Shelley, > > I would suggest you join NSH, they have all kinds of reference material > for this type of work. > > Please tell me you are off the bench, you have a lot to monitor and if > you are working the bench on top of your management duties my prayers go > out to you. > > Embedding: > > 1. Proper size of mold in relation to specimen size. > 2. Proper orientation of tissue, example 5 skin biopsies, dermis must > face the same direction, and be at an angle to the blade so when you cut > the section cuts smoothly and doesn't roll up. > 3. Multiple pieces all on the same plane. If one piece is deeper than > the others you must re-embed, or you will cut through the other pieces > before you reach it. > 4. Make sure that the embedding unit is wipe down between each case as > are the forceps, this will avoid tissue floaters. > 5. Never open more than one cassette at a time. > 6. Verify that the piece count on the work sheet matches what is in the > cassette when it is opened. > 7. Never hound the staff about speed, accuracy is more important, speed > comes with experience. If its embedded wrong, it will be cut wrong and > this will effect diagnosis. > 8. What do you do for QA on the slides?I have a work sheet that the > Pathologist fills out each day about the slides, which is the end > product of embedding. > > I hope my tips help you and feel free to contact me if you need > anything. > > Tom Podawiltz, HT (ASCP) > Histology Section Head/Laboratory Safety Officer LRGHealthcare > 603-524-3211 ext: 3220 > ________________________________________ > > > > NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a > doctor accepting new patients. Call (785) 354-5225. > > ************************************************************************ > ****************************************** > > The information transmitted in this e-mail and in any replies and > forwards are for the sole use of the above individual(s) or entities and > may contain proprietary, privileged and/or highly confidential > information. Any unauthorized dissemination, review, distribution or > copying of these communications is strictly prohibited. If this e-mail > has been transmitted to you in error, please notify and return the > original message to the sender immediately at the above listed address. > Thank you for your cooperation. > > ************************************************************************ > ****************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ________________________________ > Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. > ________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgriffo <@t> u.washington.edu Fri Aug 26 13:40:11 2011 From: mgriffo <@t> u.washington.edu (Maureen Griffo) Date: Fri Aug 26 13:40:14 2011 Subject: [Histonet] Fwd: Re: Monday deployed changes to worklist processing In-Reply-To: <4E57DD7B.7080708@pathology.washington.edu> References: <4E57DD7B.7080708@pathology.washington.edu> Message-ID: <4E57E88B.7080109@u.washington.edu> All, Jeff's email below is a message that they have successfully re-directed the ICC recut (automatic H&E request) to the IP worklist now. The unstained slides have also been re-directed there as the majority of these requests are for IHC and or FISH. This lets all have the same visual around the unstained slides that were ordered and cut. Both teams need to keep your eyes on this and get feedback to us promptly if you see any issues. Thanks much! Let me know of your questions. Maureen -------- Original Message -------- Subject: Re: Monday deployed changes to worklist processing Date: Fri, 26 Aug 2011 10:52:59 -0700 From: Jeff Cochran To: Maureen Griffo Yep. You coming here? Me go to your place? On 8/26/2011 10:41 AM, Maureen Griffo wrote: > Hi Jeff, > > Do you have a moment to chat about this in ~15 minutes? > > Thanks, > > Maureen > > On 8/25/2011 9:19 AM, Jeff Cochran wrote: >> Maureen, >> >> Monday morning (while the Bond is being restarted), we'll deploy the >> alterations to the Bond interface that allow: >> >> 1) Non-staining orders to appear on a worklist monitored for the >> Bond. Orders ICC US and ICC Recut will appear on the IP Worksheet >> worklist with no messages directed to the Bond. Worklist modification >> to orders for tests on the Bond will have the same affect as current. >> For example, moving an order from IP Worksheet to another ICC >> worklist will send a cancel-order message to the Bond (common for >> managing research cases). Worklist modification to orders for tests >> not implemented on the Bond will behave correctly (e.g., moving an >> order for FISH to the IP Worksheet worklist will not cause a message >> to be sent to the Bond). >> >> 2) Procedures configured both by worklist (in routing) and through >> declaration of a "protocol name" for the Bond. The "protocol name" is >> the Bond's "public name" for a test. The feature allows multiple LIS >> procedures mapped to a single Bond test, supporting the CAP-directed >> alterations in resulting for breast panel tests. >> >> As part of the rework to add these new features, significant work has >> been done to increase stability and scalability in the Bond >> interface, and to access more LIS data when auto-generating negative >> control and H&E orders. >> >> Please let everyone know and please ask that they keep their eyes >> open for any problems on Monday. I'll be keeping the day open for any >> problems that may arise and the team should feel free to grab me or, >> preferably, open a high-priority tech request if they need to. >> >> Thanks, >> >> Jeff >> -- Jeff Cochran Senior Computer Specialist University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 (206) 598-1616 (206) 598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From SJMccabe <@t> drmc.org Fri Aug 26 13:48:30 2011 From: SJMccabe <@t> drmc.org (McCabe, Sara J.) Date: Fri Aug 26 13:50:54 2011 Subject: [Histonet] CMV IHC Message-ID: <02AE2390303AAB43A823930EAD6B63AE1C46B9D5@EX07.drmc.org> Happy Friday Histonetters! Anyone performing CMV by IHC? If so what are you using for a protocol? We ordered CMV from Cell Marque but they give no suggestions for a protocol. We are using the Leica Bond for our IHCs. Any input is greatly appreciated! Thanks! Sara McCabe, HT (ASCP) DuBois Regional Medical Center ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, August 26, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 93, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Embedding Center Questions (Tim Wheelock) 2. Embedding competency and med techs running a Histo lab (D'Attilio, Shelley) 3. RE: AMPHYL (Sebree Linda A) 4. RE: Embedding Center Questions (Rathborne, Toni) 5. Jill's MSDS question: a tech's perspective (Cheryl) 6. RE: heavy duty microtome (Jack Ratliff) 7. RE: AMPHYL (McMahon, Loralee A) 8. AUTO: Gina Rodriguez is out of the office. (returning 09/06/2011) (Gina.Rodriguez@leica-microsystems.com) 9. OT: Hurricane Irene (Pam Barker) 10. RE: AMPHYL (Shirley A. Powell) 11. Re: Jill's MSDS question: a tech's perspective (Jill Cox) 12. Re: Jill's MSDS question: a tech's perspective (Emily Sours) 13. Re: Embedding process etc. (Bob Richmond) ---------------------------------------------------------------------- Message: 1 Date: Fri, 26 Aug 2011 09:54:27 -0400 From: Tim Wheelock Subject: [Histonet] Embedding Center Questions To: histonet@lists.utsouthwestern.edu Message-ID: <4E57A593.90208@mclean.harvard.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi All: My 23 year old Shandon Embedding Center may be ready to give up the ghost. It has had two repairs of the coolant system for the freezing plate over the last year. Now a thermo-resistor has burnt out, so that the wax reservoir with no long melt the wax. Does anyone know of a company or companies that lease/rent histology equipment? Are there companies who sell used equipment? Also, what do people recommend as far as a new embedding center, in case we can afford it? I am in the Boston area. In the meantime, maybe I can jury-rig something, since it is only the wax reservoir that is down. The cassette holding tank, hot plate, and freezing plate are OK for the moment. Maybe I can melt a few jars of wax, and use a transfer pipet to fill the molds? Any ideas? Thanks, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 ------------------------------ Message: 2 Date: Fri, 26 Aug 2011 08:57:35 -0500 From: "D'Attilio, Shelley" Subject: [Histonet] Embedding competency and med techs running a Histo lab To: "Histonet Listserv (E-mail)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi all, Thanks so much for the advice I received following my request for help on our embedding competency improvement project. I enjoyed the lively discussion that followed about non-histotechs managing a Histology lab and I appreciated all the kind comments and words of encouragement both on and off the listserv board. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** ------------------------------ Message: 3 Date: Fri, 26 Aug 2011 09:02:24 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] AMPHYL To: "Pam Marcum" , "Rae Staskiewicz" Cc: histonet@lists.utsouthwestern.edu, Barbara Crill Message-ID: Content-Type: text/plain; charset="us-ascii" We've been getting our Lysol IC from Cardinal Health but in the past we've had to go through Amazon.com Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ________________________________ From: Pam Marcum [mailto:mucram11@comcast.net] Sent: Friday, August 26, 2011 8:39 AM To: Rae Staskiewicz Cc: Sebree Linda A; Barbara Crill; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] AMPHYL We can't find Lysol IC here either so any supplier would help and to keep costs down a store would be better than a scientific supplier it possible. Please id anyone knows let us all know as it is not easy to find. Pam Marcum ________________________________ From: "Rae Staskiewicz" To: "Sebree Linda A" , "Barbara Crill" , histonet@lists.utsouthwestern.edu Sent: Thursday, August 25, 2011 4:53:36 PM Subject: RE: [Histonet] AMPHYL Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 26 Aug 2011 14:10:32 +0000 From: "Rathborne, Toni" Subject: RE: [Histonet] Embedding Center Questions To: "'Tim Wheelock'" , "histonet@lists.utsouthwestern.edu" Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F53E0C@SMCMAIL01.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" A couple of places that come to mind are Belair (800-783-9424), Medical Equipment Source (www.medequipsource.com), and Mayflower (800-287-9801). I know that they sell used equipment, you could inquire about a rental. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, August 26, 2011 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Center Questions Hi All: My 23 year old Shandon Embedding Center may be ready to give up the ghost. It has had two repairs of the coolant system for the freezing plate over the last year. Now a thermo-resistor has burnt out, so that the wax reservoir with no long melt the wax. Does anyone know of a company or companies that lease/rent histology equipment? Are there companies who sell used equipment? Also, what do people recommend as far as a new embedding center, in case we can afford it? I am in the Boston area. In the meantime, maybe I can jury-rig something, since it is only the wax reservoir that is down. The cassette holding tank, hot plate, and freezing plate are OK for the moment. Maybe I can melt a few jars of wax, and use a transfer pipet to fill the molds? Any ideas? Thanks, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 5 Date: Fri, 26 Aug 2011 07:17:08 -0700 (PDT) From: Cheryl Subject: [Histonet] Jill's MSDS question: a tech's perspective To: "histonet@lists.utsouthwestern.edu" Message-ID: <1314368228.65620.YahooMailNeo@web39410.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Jill-- Congrats on the job! ? The MSDS in paper vs. eCopy?copy will come down to the opinion of your inspector.? Like Wil says, he's passed before...and I've passed before but only because we had both eCopies and hard copies.? Most of the larger for-profit and major health system administrations?require both by SOP regardless, for liability reasons usually but please read on! ? Better yet, think of the practical uses and real-world applications of?this?rather than the 'do it because I have to' ones: ? If you or one of yours gets splashed with something--how do you want the next response to go?? If you only have eCopies and the power is down, or you?are having computer issues, or the PC is slow, or?the printer on the network isn't responding, then what? If it's your eyes or your skin that's about to be irreversably burnt, wouldn't you want that information available in a TOTALLY reliable manner and a copy available to?grab,?follow?AND send to the emergency room with you--RIGHT NOW?? Then add to it the liability for your company should this be the case...and we all know when you most need your PC to work is when it doesn't. ? If I'm having trouble making a decision often exaggerating the potential circumstances makes the answer far easier to come to. ? If I am working in a lab I want hard copies alphabetized by both the mfc. chemical name and the common name we use for it in the lab with an index?with the binders (Print the covers black on yellow paper and insert these in clear view binders)?on the benches in the space where we work.? Not on a computer or off in the transcription area.? It may not be required by your inspector or by your facility, but is that really why we have these in our labs in the first place--because we're required to do so?? ? My four cents.... ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc - Staffing the AP Lab one GREAT tech at a time. 281.852.9457 admin@fullstaff.org ------------------------------ Message: 6 Date: Fri, 26 Aug 2011 10:24:46 -0400 From: Jack Ratliff Subject: RE: [Histonet] heavy duty microtome To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I know of two possible options for exactly what you seek. Please contact me directly so that we may point you in the right direction and discuss with you the details. Best Regards, Jack PS I have heard a rumor that these units will no longer be for sale by Leica and no replacements will be available in the future. Additionally, I very seriously doubt that they have a demo unit for sale as they typically manufacture these units upon approved purchase order. Jack L Ratliff Senior Histologist, BioMimetic Therapeutics, Inc. Chair, Hard Tissue Committee - National Society for Histotechnology > From: JBAR@ANA.AU.DK > To: histonet@lists.utsouthwestern.edu > Date: Fri, 26 Aug 2011 09:08:29 +0200 > Subject: [Histonet] heavy duty microtome > > Does anyone have either a used Leica Polycut E or SM 2500 for sale. > For the present we are using a very old (50 years) Reichert-Jung heavy duty microtome and looking for a replacement. > > If you can help us please contact: > > Jette Barlach > Research unit for Rheumatology an Bone biology > Dept. of Rheumatology > University hospital of Aarhus > N?rrebrogade 44 > 8000 Aarhus C > Denmark > jbar@ana.au.dk > +45 89 42 29 92 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 26 Aug 2011 10:33:18 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] AMPHYL To: Pam Marcum , Rae Staskiewicz Cc: "histonet@lists.utsouthwestern.edu" , Sebree Linda A , Barbara Crill Message-ID: Content-Type: text/plain; charset="us-ascii" http://www.labsafety.com/lysol-ic-quaternary-cleaner-disinfectant_24530427/ Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum [mucram11@comcast.net] Sent: Friday, August 26, 2011 9:39 AM To: Rae Staskiewicz Cc: histonet@lists.utsouthwestern.edu; Barbara Crill; Sebree Linda A Subject: Re: [Histonet] AMPHYL We can't find Lysol IC here either so any supplier would help and to keep costs down a store would be better than a scientific supplier it possible. Please id anyone knows let us all know as it is not easy to find. Pam Marcum ----- Original Message ----- From: "Rae Staskiewicz" To: "Sebree Linda A" , "Barbara Crill" , histonet@lists.utsouthwestern.edu Sent: Thursday, August 25, 2011 4:53:36 PM Subject: RE: [Histonet] AMPHYL Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 26 Aug 2011 10:00:52 -0500 From: Gina.Rodriguez@leica-microsystems.com Subject: [Histonet] AUTO: Gina Rodriguez is out of the office. (returning 09/06/2011) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I am out of the office until 09/06/2011. I will respond to your message when I return. Note: This is an automated response to your message "Histonet Digest, Vol 93, Issue 35" sent on 8/26/2011 8:45:07 AM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 9 Date: Fri, 26 Aug 2011 11:07:21 -0400 From: "Pam Barker" Subject: [Histonet] OT: Hurricane Irene To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! Are you in the projected path of Irene? I'm a long time florida resident who has weathered hurricanes&false alarms in both central Florida&the panhandle. It's not the hurricane it's the aftermath... No power which means no gasoline no atms no A.C. no lights, no water. At least fill your car with gas have cash on hand&fill your bathtub with water That way you are prepared and have not spent alot of money on stuff you wouldn't use on the OFF chance that it doesn't affect you. Be Safe!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 10 Date: Fri, 26 Aug 2011 11:07:19 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] AMPHYL To: Pam Marcum , Rae Staskiewicz Cc: "histonet@lists.utsouthwestern.edu" , Sebree Linda A , Barbara Crill Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE438B6E@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="utf-8" Google Lysol IC and you will get plenty of suppliers. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Friday, August 26, 2011 9:39 AM To: Rae Staskiewicz Cc: histonet@lists.utsouthwestern.edu; Barbara Crill; Sebree Linda A Subject: Re: [Histonet] AMPHYL We can't find Lysol IC here either so any supplier would help and to keep costs down a store would be better than a scientific supplier it possible.?? Please id anyone knows let us all know as it is not easy to find. Pam Marcum ----- Original Message ----- From: "Rae Staskiewicz" To: "Sebree Linda A" , "Barbara Crill" , histonet@lists.utsouthwestern.edu Sent: Thursday, August 25, 2011 4:53:36 PM Subject: RE: [Histonet] AMPHYL Linda, Where do you get your Lysol IC? Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? ??Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 26 Aug 2011 08:21:49 -0700 (PDT) From: Jill Cox Subject: Re: [Histonet] Jill's MSDS question: a tech's perspective To: Cheryl , "Histonet@Lists. Edu" Message-ID: <1314372109.6931.YahooMailNeo@web161605.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Cheryl, Thanks! I do have hard copies here, my main question was about the binder color. I ended up ordering a MSDS binder from LSS yesterday. I just wanted all manuals to match, lol. I haven't had hard copy in years, everywhere I have been has only had online and saved to desktop. We did pass all inspections with online only. It's great to hear from you! Jill Cox, HT ASCP ? ? From: Cheryl To: "histonet@lists.utsouthwestern.edu" Sent: Friday, August 26, 2011 7:17 AM Subject: [Histonet] Jill's MSDS question: a tech's perspective Hi Jill-- Congrats on the job! ? The MSDS in paper vs. eCopy?copy will come down to the opinion of your inspector.? Like Wil says, he's passed before...and I've passed before but only because we had both eCopies and hard copies.? Most of the larger for-profit and major health system administrations?require both by SOP regardless, for liability reasons usually but please read on! ? Better yet, think of the practical uses and real-world applications of?this?rather than the 'do it because I have to' ones: ? If you or one of yours gets splashed with something--how do you want the next response to go?? If you only have eCopies and the power is down, or you?are having computer issues, or the PC is slow, or?the printer on the network isn't responding, then what? If it's your eyes or your skin that's about to be irreversably burnt, wouldn't you want that information available in a TOTALLY reliable manner and a copy available to?grab,?follow?AND send to the emergency room with you--RIGHT NOW?? Then add to it the liability for your company should this be the case...and we all know when you most need your PC to work is when it doesn't. ? If I'm having trouble making a decision often exaggerating the potential circumstances makes the answer far easier to come to. ? If I am working in a lab I want hard copies alphabetized by both the mfc. chemical name and the common name we use for it in the lab with an index?with the binders (Print the covers black on yellow paper and insert these in clear view binders)?on the benches in the space where we work.? Not on a computer or off in the transcription area.? It may not be required by your inspector or by your facility, but is that really why we have these in our labs in the first place--because we're required to do so?? ? My four cents.... ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc - Staffing the AP Lab one GREAT tech at a time. 281.852.9457 admin@fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 26 Aug 2011 11:29:20 -0400 From: Emily Sours Subject: Re: [Histonet] Jill's MSDS question: a tech's perspective To: Cheryl , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 I would respond to this by saying, if I were working with something that dangerous, I would know ahead of time what to do with it. I'm not going to spend any amount of time looking up an MSDS if something is on me RIGHT NOW, digital or not. Which is why I think it makes sense to have digital copies, because you're going to know how to handle hazardous stuff and digital copies save space and time. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Aug 26, 2011 at 10:17 AM, Cheryl wrote: > Hi Jill-- Congrats on the job! > > The MSDS in paper vs. eCopy copy will come down to the opinion of your > inspector. Like Wil says, he's passed before...and I've passed before but > only because we had both eCopies and hard copies. Most of the larger > for-profit and major health system administrations require both by SOP > regardless, for liability reasons usually but please read on! > > Better yet, think of the practical uses and real-world applications > of this rather than the 'do it because I have to' ones: > > If you or one of yours gets splashed with something--how do you want the > next response to go? If you only have eCopies and the power is down, or > you are having computer issues, or the PC is slow, or the printer on the > network isn't responding, then what? If it's your eyes or your skin that's > about to be irreversably burnt, wouldn't you want that information available > in a TOTALLY reliable manner and a copy available to grab, follow AND send > to the emergency room with you--RIGHT NOW? Then add to it the liability for > your company should this be the case...and we all know when you most need > your PC to work is when it doesn't. > > If I'm having trouble making a decision often exaggerating the potential > circumstances makes the answer far easier to come to. > > If I am working in a lab I want hard copies alphabetized by both the mfc. > chemical name and the common name we use for it in the lab with an > index with the binders (Print the covers black on yellow paper and insert > these in clear view binders) on the benches in the space where we work. Not > on a computer or off in the transcription area. It may not be required by > your inspector or by your facility, but is that really why we have these in > our labs in the first place--because we're required to do so? > > My four cents.... > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc - Staffing the AP Lab one GREAT tech at a time. > 281.852.9457 > admin@fullstaff.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Fri, 26 Aug 2011 11:36:15 -0400 From: Bob Richmond Subject: [Histonet] Re: Embedding process etc. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Bill O'Donnell observes: >>I once worked for a very demanding pathologist and because of his expectations, the whole crew put out near perfection. Pathologists that are "OK" with what they get, so long as they can make a diagnosis, are a huge part of that problem.<< I very much agree, though I've often been guilty as charged. Edwards Deming - whom of course American MBA's learn to ridicule - was very much concerned with constant feedback, and constant attention to fixing little problems before they turn into big problems. The histotechnologist who never looks at a slide and the pathologist who is afraid to complain about unsatisfactory work are two sides of the same problem. This month's Journal of Histotechnology has an article about quality assurance of GI biopsies that everyone concerned with this issue should read. I'll try to get review of it onto Histonet. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 93, Issue 36 **************************************** From Vickroy.Jim <@t> mhsil.com Fri Aug 26 14:22:54 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Aug 26 14:23:07 2011 Subject: [Histonet] ER/PR validation Message-ID: <24A4826E8EF0964D86BC5317306F58A55FC3CFEAFB@mmc-mail.ad.mhsil.com> After my initial question a couple of days ago it is apparent that we should sure up our validation of ER/PR. We are currently using the FDA approved method from Ventana and would like to find someone to partner with to perform ER/PR stains on 20 of our positive cases and 20 of our negative cases. I believe that by doing this both of us could satisfy the requirements of this new CAP requirement. I have pulled my CAP survey cases (PMK's) and most likely have documentation of plenty of positive cases but the number of negative cases is lacking. If you are interested in partnering with us so that both of us could meet the requirements please let me know. I am also looking into help from a couple of companies that routinely do ER/PR testing. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From TGoins <@t> mt.gov Fri Aug 26 15:48:32 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Aug 26 15:48:40 2011 Subject: [Histonet] RE: MSDS In-Reply-To: References: <1314368228.65620.YahooMailNeo@web39410.mail.mud.yahoo.com> Message-ID: The hard copy of the MSDS is for your own safety AND for the safety of any person responding to an emergency. If you work with the reagent, you should be familiar with the associated hazards. Someone from down the hall or emergency response personnel will not necessarily be familiar with the associated hazards. I don't know about you, but I sometimes have trouble locating files on my own computer - I would hate to depend on someone else trying to find a MSDS file on a computer prior to administering aid. The skill of access by alphabetical means is something everyone is familiar with - a hard copy of the MSDS is a necessity. My two cents, Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, August 26, 2011 9:29 AM To: Cheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Jill's MSDS question: a tech's perspective I would respond to this by saying, if I were working with something that dangerous, I would know ahead of time what to do with it. I'm not going to spend any amount of time looking up an MSDS if something is on me RIGHT NOW, digital or not. Which is why I think it makes sense to have digital copies, because you're going to know how to handle hazardous stuff and digital copies save space and time. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Aug 26, 2011 at 10:17 AM, Cheryl wrote: > Hi Jill-- Congrats on the job! > > The MSDS in paper vs. eCopy copy will come down to the opinion of your > inspector. Like Wil says, he's passed before...and I've passed before but > only because we had both eCopies and hard copies. Most of the larger > for-profit and major health system administrations require both by SOP > regardless, for liability reasons usually but please read on! > > Better yet, think of the practical uses and real-world applications > of this rather than the 'do it because I have to' ones: > > If you or one of yours gets splashed with something--how do you want the > next response to go? If you only have eCopies and the power is down, or > you are having computer issues, or the PC is slow, or the printer on the > network isn't responding, then what? If it's your eyes or your skin that's > about to be irreversably burnt, wouldn't you want that information available > in a TOTALLY reliable manner and a copy available to grab, follow AND send > to the emergency room with you--RIGHT NOW? Then add to it the liability for > your company should this be the case...and we all know when you most need > your PC to work is when it doesn't. > > If I'm having trouble making a decision often exaggerating the potential > circumstances makes the answer far easier to come to. > > If I am working in a lab I want hard copies alphabetized by both the mfc. > chemical name and the common name we use for it in the lab with an > index with the binders (Print the covers black on yellow paper and insert > these in clear view binders) on the benches in the space where we work. Not > on a computer or off in the transcription area. It may not be required by > your inspector or by your facility, but is that really why we have these in > our labs in the first place--because we're required to do so? > > My four cents.... > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc - Staffing the AP Lab one GREAT tech at a time. > 281.852.9457 > admin@fullstaff.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dr_sadushe <@t> yahoo.com Fri Aug 26 15:50:45 2011 From: dr_sadushe <@t> yahoo.com (Sadushe Loxha) Date: Fri Aug 26 16:45:58 2011 Subject: [Histonet] (no subject) Message-ID: <1314391845.61646.YahooMailMobile@web36103.mail.mud.yahoo.com> http://www.davitt4council.com/wp-content/themes/delegate/vdfzblkk.htm From brent.jeter <@t> gwu-hospital.com Fri Aug 26 17:24:45 2011 From: brent.jeter <@t> gwu-hospital.com (Jeter, Brent) Date: Fri Aug 26 17:24:53 2011 Subject: [Histonet] Cleanser for blades/instruments Message-ID: <3AD1556A3658A240AADF7BD8E805086C0142A640EDF3@CORP-EXVS03.corp.uhsinc.biz> Histonetters, Can anyone recommend a good cleanser for soaking scissors/blades/instruments? Thanks in advance - Brent Jeter Anatomic Pathology Supervisor The George Washington University Hospital 202-715-5076 (phone) 202-715-4691 (fax) brent.jeter@gwu-hospital.com UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. From mehlikafaire <@t> hotmail.com Fri Aug 26 17:46:04 2011 From: mehlikafaire <@t> hotmail.com (mehlika faire) Date: Fri Aug 26 17:46:11 2011 Subject: [Histonet] Does anyone know of a good protocol for pERK staining in PFA fixed tissues? Message-ID: Hello All, I was wondering if anyone has a good protocol for pERK staining in PFA fixed tissues. I have tried several protocols (including Antigen retrieval using trypsin and dnase) but have been unsuccessful so far. I am trying to identify pERK in 10uM sections that have been fixed in 4% PFA for 1 hour. If anyone has any insight, I would really appreciate it! Thanks in advance! Best, Mehlika From histotech <@t> imagesbyhopper.com Fri Aug 26 19:40:02 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Aug 26 19:40:19 2011 Subject: [Histonet] Cleanser for blades/instruments In-Reply-To: <3AD1556A3658A240AADF7BD8E805086C0142A640EDF3@CORP-EXVS03.corp.uhsinc.biz> References: <3AD1556A3658A240AADF7BD8E805086C0142A640EDF3@CORP-EXVS03.corp.uhsinc.biz> Message-ID: Liquinox and bleach soulution works for us. Michelle Sent from my iPhone On Aug 26, 2011, at 6:24 PM, "Jeter, Brent" wrote: > Histonetters, > > Can anyone recommend a good cleanser for soaking scissors/blades/instruments? > > Thanks in advance - > > Brent Jeter > Anatomic Pathology Supervisor > The George Washington University Hospital > 202-715-5076 (phone) > 202-715-4691 (fax) > brent.jeter@gwu-hospital.com > > > > UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Fri Aug 26 22:05:15 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 26 22:05:21 2011 Subject: [Histonet] Histonet] Re: Embedding process etc. Message-ID: Hi, That was a good article. I really do hope I am not the only one that was just mortified at the high numbers of bad sections being produced. Once in a while on a bit of difficult tissue is one thing, but consistent poor quality is inexcusable. Come to think of it that was actually a rather depressing article. Amos On Fri, Aug 26, 2011 at 1:00 PM, wrote: > Message: 13 > Date: Fri, 26 Aug 2011 11:36:15 -0400 > From: Bob Richmond > Subject: [Histonet] Re: Embedding process etc. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > Bill O'Donnell observes: > > >>I once worked for a very demanding pathologist and because of his > expectations, the whole crew put out near perfection. Pathologists that are > "OK" with what they get, so long as they can make a diagnosis, are a huge > part of that problem.<< > > I very much agree, though I've often been guilty as charged. Edwards > Deming - whom of course American MBA's learn to ridicule - was very > much concerned with constant feedback, and constant attention to > fixing little problems before they turn into big problems. > > The histotechnologist who never looks at a slide and the pathologist > who is afraid to complain about unsatisfactory work are two sides of > the same problem. > > This month's Journal of Histotechnology has an article about quality > assurance of GI biopsies that everyone concerned with this issue > should read. I'll try to get review of it onto Histonet. > > Bob Richmond > Samurai Pathologist > Knoxville TN > From rjbuesa <@t> yahoo.com Sat Aug 27 10:22:18 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Aug 27 10:22:22 2011 Subject: [Histonet] Cleanser for blades/instruments In-Reply-To: <3AD1556A3658A240AADF7BD8E805086C0142A640EDF3@CORP-EXVS03.corp.uhsinc.biz> Message-ID: <1314458538.57318.YahooMailClassic@web65706.mail.ac4.yahoo.com> Immersion during 5 minutes (twice)?in a? 2% aq. v/v solution of a lab strength detergent at 90?C?.Ren? J. --- On Fri, 8/26/11, Jeter, Brent wrote: From: Jeter, Brent Subject: [Histonet] Cleanser for blades/instruments To: "histonet@lists.utsouthwestern.edu" Date: Friday, August 26, 2011, 6:24 PM Histonetters, Can anyone recommend a good cleanser for soaking scissors/blades/instruments? Thanks in advance - Brent Jeter Anatomic Pathology Supervisor The George Washington University Hospital 202-715-5076 (phone) 202-715-4691 (fax) brent.jeter@gwu-hospital.com UHS Confidentiality Notice:? This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information.? Any unauthorized review, use, disclosure or distribution of this information is prohibited.? If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From crochieresteve <@t> aol.com Sat Aug 27 11:28:03 2011 From: crochieresteve <@t> aol.com (Steven) Date: Sat Aug 27 11:28:12 2011 Subject: [Histonet] (no subject) Message-ID: <8CE32ED6D5D24AB-13B0-11C3D@webmail-d061.sysops.aol.com> http://www.baggageproductions.com/wp-content/themes/boast/hgjdll.htm From crochieresteve <@t> aol.com Sat Aug 27 11:43:10 2011 From: crochieresteve <@t> aol.com (Steven) Date: Sat Aug 27 11:43:18 2011 Subject: [Histonet] (no subject) Message-ID: <8CE32EF8A17866E-1ACC-1AAE5@webmail-d177.sysops.aol.com> http://autodoplnky.prnews.cz/oldtemp/hgjdll.htm From lpwenk <@t> sbcglobal.net Sat Aug 27 12:45:43 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Aug 27 12:45:36 2011 Subject: [Histonet] Returning to Histology-Another Idea Won't Work In-Reply-To: <1314300719.14987.YahooMailClassic@web30304.mail.mud.yahoo.com> References: <1314300719.14987.YahooMailClassic@web30304.mail.mud.yahoo.com> Message-ID: <0C4105D4F31A489792A55F56E30ED47B@HP2010> "It looks like the time limits prohibit me from trying this, since I did it so long ago" What is "IT"? What is prohibiting you from taking the HTL exam? What does having taken and passed the HT exam have to do with taking the HTL exam? Sorry, I'm really lost as to what you need, and how to help you. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 "The above response reflects my opinion, and not the hospital's view." -----Original Message----- From: Paula Sent: Thursday, August 25, 2011 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Returning to Histology-Another Idea Won't Work I thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either: Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; OR Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. *laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.) Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Any ideas? I have posted this before, but I keep on trying! Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madary <@t> verizon.net Sat Aug 27 14:25:56 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Sat Aug 27 14:25:59 2011 Subject: [Histonet] harrasment for humble histotechs Message-ID: <33099009.1060937.1314473156376.JavaMail.root@vznit170132> Gentle harrassment. I encourage you to look at you scores for your practical and written exam. Between gigs I am getting my important documents together and found my old scores for the HTL, no thte HT, however, both parts of the exam were taken by yours truly. Never got into the talk of practical vs no practical. I do think it si a good idea to keep it. Honest techs will be proud to do a practical. Looking at scores made me feel good that the next great job is right around the corner. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC From histotech <@t> imagesbyhopper.com Sat Aug 27 15:50:26 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Aug 27 15:50:36 2011 Subject: [Histonet] harrasment for humble histotechs In-Reply-To: <33099009.1060937.1314473156376.JavaMail.root@vznit170132> References: <33099009.1060937.1314473156376.JavaMail.root@vznit170132> Message-ID: <840D06FE-5A74-40F7-AA1F-32751738600F@imagesbyhopper.com> :o). I'm not even sure I could find my results after all these years and 9 moves! I *do* remember how picture perfect those slides had to be though. Tiny air bubble=graded down. Folds=forget it. Knife marks=bad. I did both the HT and the HTL practicals and now new students don't even have to submit them. I didn't cheat, but over the years I saw students attempting to cheat. If I saw them, I would remind them that they were to work independently, but who knows what they did when I wasn't looking? Integrity is what you do when no one is looking. Sent from my iPhone On Aug 27, 2011, at 3:25 PM, madary@verizon.net wrote: > > Gentle harrassment. I encourage you to look at you scores for your > practical and written exam. Between gigs I am getting my important > documents together and found my old scores for the HTL, no thte HT, > however, both parts of the exam were taken by yours truly. Never got > into the talk of practical vs no practical. I do think it si a good > idea to keep it. Honest techs will be proud to do a practical. Looking > at scores made me feel good that the next great job is right around > the corner. > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From k84as <@t> yahoo.com Sat Aug 27 17:59:32 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sat Aug 27 17:59:35 2011 Subject: [Histonet] (no subject) Message-ID: <1314485972.320.YahooMailMobile@web112613.mail.gq1.yahoo.com> http://www.scoutpack236.com/wp-includes/tzzzgg.htm From campbellj <@t> muhlbauerlab.com Sat Aug 27 18:27:32 2011 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Sat Aug 27 18:27:36 2011 Subject: [Histonet] harrasment for humble histotechs In-Reply-To: <840D06FE-5A74-40F7-AA1F-32751738600F@imagesbyhopper.com> References: <33099009.1060937.1314473156376.JavaMail.root@vznit170132> <840D06FE-5A74-40F7-AA1F-32751738600F@imagesbyhopper.com> Message-ID: I kicked ass on the HT practical! No way I was going to go through that hassle again. I am still puzzled as to why the powers that be got rid of it. It definitely tested how well you could follow directions too. On Sat, Aug 27, 2011 at 4:50 PM, histotech@imagesbyhopper.com < histotech@imagesbyhopper.com> wrote: > :o). I'm not even sure I could find my results after all these years and 9 > moves! > > I *do* remember how picture perfect those slides had to be though. Tiny air > bubble=graded down. Folds=forget it. Knife marks=bad. > > I did both the HT and the HTL practicals and now new students don't even > have to submit them. I didn't cheat, but over the years I saw students > attempting to cheat. If I saw them, I would remind them that they were to > work independently, but who knows what they did when I wasn't looking? > > Integrity is what you do when no one is looking. > > > > Sent from my iPhone > > On Aug 27, 2011, at 3:25 PM, madary@verizon.net wrote: > > > > > Gentle harrassment. I encourage you to look at you scores for your > > practical and written exam. Between gigs I am getting my important > > documents together and found my old scores for the HTL, no thte HT, > > however, both parts of the exam were taken by yours truly. Never got > > into the talk of practical vs no practical. I do think it si a good > > idea to keep it. Honest techs will be proud to do a practical. Looking > > at scores made me feel good that the next great job is right around > > the corner. > > > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 From histotech <@t> imagesbyhopper.com Sat Aug 27 19:06:18 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Aug 27 19:06:29 2011 Subject: [Histonet] Returning to Histology-Another Idea Won't Work In-Reply-To: <0C4105D4F31A489792A55F56E30ED47B@HP2010> References: <1314300719.14987.YahooMailClassic@web30304.mail.mud.yahoo.com> <0C4105D4F31A489792A55F56E30ED47B@HP2010> Message-ID: <65225DD0-8177-48E4-A022-D3C8A3D84948@imagesbyhopper.com> 10 years experience to qualify for taking the exam? Is that an ASCP requirement? It sounds like they might be exchanging experience for the bachelor's degree? Have you checked out the online courses? I believe there are some that offer the bachelor's degree. Michelle Sent from my iPhone On Aug 27, 2011, at 1:45 PM, "Lee & Peggy Wenk" wrote: > "It looks like the time limits prohibit me from trying this, since I did it so long ago" > > What is "IT"? What is prohibiting you from taking the HTL exam? What does having taken and passed the HT exam have to do with taking the HTL exam? > > Sorry, I'm really lost as to what you need, and how to help you. > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospital > Royal Oak, MI 48073 > > "The above response reflects my opinion, and not the hospital's view." > > -----Original Message----- From: Paula > Sent: Thursday, August 25, 2011 3:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Returning to Histology-Another Idea Won't Work > > I thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either: > > Histotechnologist, HTL(ASCP) > > Application Fee: $210 > > To be eligible for this examination category, an applicant must > satisfy the requirements of at least one of the following routes: > > Route 1: Baccalaureate degree from a regionally > accredited college/university with a combination of 30 semester hours > (45 quarter hours) of biology and chemistry AND successful completion of > a NAACLS accredited Histotechnician or Histotechnology program within > the last 5 years; OR > > Route 2: Baccalaureate degree from a regionally > accredited college/university with a combination of 30 semester hours > (45 quarter hours) of biology and chemistry AND one year full time > acceptable experience in a histopathology (clinical, veterinary, > industry or research) laboratory in the U.S., Canada or an accredited > laboratory* within the last ten years. > > *laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.) > > Clinical Laboratory Experience > > To fulfill the experience requirement for the Histotechnologist > examination, you must have experience, within the last ten years, in the > following areas: > > Fixation > Microtomy > Processing > Staining Any ideas? I have posted this before, but I keep on trying! > > Paula > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotech <@t> imagesbyhopper.com Sat Aug 27 19:07:32 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Aug 27 19:07:42 2011 Subject: [Histonet] Returning to Histology-Another Idea Won't Work In-Reply-To: <0C4105D4F31A489792A55F56E30ED47B@HP2010> References: <1314300719.14987.YahooMailClassic@web30304.mail.mud.yahoo.com> <0C4105D4F31A489792A55F56E30ED47B@HP2010> Message-ID: Sorry, I misread, the experience must be within the last 10 years. Michelle Sent from my iPhone On Aug 27, 2011, at 1:45 PM, "Lee & Peggy Wenk" wrote: > "It looks like the time limits prohibit me from trying this, since I did it so long ago" > > What is "IT"? What is prohibiting you from taking the HTL exam? What does having taken and passed the HT exam have to do with taking the HTL exam? > > Sorry, I'm really lost as to what you need, and how to help you. > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospital > Royal Oak, MI 48073 > > "The above response reflects my opinion, and not the hospital's view." > > -----Original Message----- From: Paula > Sent: Thursday, August 25, 2011 3:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Returning to Histology-Another Idea Won't Work > > I thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either: > > Histotechnologist, HTL(ASCP) > > Application Fee: $210 > > To be eligible for this examination category, an applicant must > satisfy the requirements of at least one of the following routes: > > Route 1: Baccalaureate degree from a regionally > accredited college/university with a combination of 30 semester hours > (45 quarter hours) of biology and chemistry AND successful completion of > a NAACLS accredited Histotechnician or Histotechnology program within > the last 5 years; OR > > Route 2: Baccalaureate degree from a regionally > accredited college/university with a combination of 30 semester hours > (45 quarter hours) of biology and chemistry AND one year full time > acceptable experience in a histopathology (clinical, veterinary, > industry or research) laboratory in the U.S., Canada or an accredited > laboratory* within the last ten years. > > *laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.) > > Clinical Laboratory Experience > > To fulfill the experience requirement for the Histotechnologist > examination, you must have experience, within the last ten years, in the > following areas: > > Fixation > Microtomy > Processing > Staining Any ideas? I have posted this before, but I keep on trying! > > Paula > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dr_sadushe <@t> yahoo.com Sun Aug 28 02:57:40 2011 From: dr_sadushe <@t> yahoo.com (Sadushe Loxha) Date: Sun Aug 28 02:57:45 2011 Subject: [Histonet] (no subject) Message-ID: <1314518260.98699.YahooMailMobile@web36102.mail.mud.yahoo.com> http://989.cz/oldtemp/tzzzgg.htm From lpwenk <@t> sbcglobal.net Sun Aug 28 07:55:33 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Aug 28 07:55:29 2011 Subject: [Histonet] Practical - 2nd try Message-ID: <2FFDBE49BDBC41EB85B638A21A0A92B7@HP2010> Trying to send again. Didn?t seem to go to HistoNet the first time, from what I can see. If you?ve seen it already, hit deleted fast. The reason the practical exam was dropped was NOT due to ASCP only having MT's. There were lots of HT/HTL associated with NSH that would be invited to grade, along with pathologists, many of whom also present at NSH. I wrote the following (after the *****), and posted it on HistoNet on March 14, 2006. So if you are not interested, hit delete now. If someone comes out of a NAACLS HT/HT program, and cannot cut or section, then it is the fault of the program. Complain to the program. Complain to the accrediting agency www.NAACLS.org I'm sure my students think I trained in the same Navy facility, as I demand perfection. My students still have to turn in a practical exam - HT being somewhat different than HTL in terms of some tissues, some sizes, some stains, numbers. - HT - 1 H&E from 23 different tissues, 7 special stains (each student gets different special stains on different tissues. Maybe they all have a PAS, but one is on sm. intestine, one on colon, one on fungus) - HTL - 1 H&E from 31 different tissues, 9 special stains, 2 IHC I have check sheets - 1 point for perfect block and slide (label, embedding, coverslipping, full face, not cut through, etc.) - if anything wronge = 0 points. - 2 points for sectioning - any cutting artifact = 0 points - 1 point misc (fixation ppt, over processing, bone dust, etc.) ? any problems = 0 points - 6-4-2-0 points for staining. (perfect, good, poor, bad) If they have on a slide with a knife line, and 2 tone eosin, that's 1-0-1-4 = 6 points. They need to have 75% overall to pass the practical. The practical is worth 10% of their grade. They have the entire practicum to work on it (7 months for the HT, 11.5 months for HTL). It's a competency based program, so failing any part (exam, homework, practical, rotations) means they don't pass, even if their overall grade is above the 75%. If people are interested in my practical grading sheets, email me directly (NOT through HistoNet), and I'll email. Specify HT (no enzymes, no IHC) or HTL (enzymes, IHC, more special stains). Below are the reasons for that the practical was dropped, that I wrote in 2006. The only thing I would add now is, if you can't "test" them for competency of sectioning, coverslipping, etc. during the interview, then USE the 3 months probation option. If they said during the interview that they knew how to microtomy, and you find once they start working that they can't - they lied - then FIRE them! BE the supervisor! Don't allow yourself to be stuck with any employee who can't do the work, won't do the work, is bad for your lab, is bad for the patient, etc. ************ Yes, the HT and HTL practical exams are being discontinued starting in 2007. In other words, 2006 is the last year it will be required. (If you're not interested, please hit "delete" at this point, as I'm going to get wordy.) There was information on the ASCP Board of Registry (BOR) web page. However, ASCP recently redid their entire web page, and this information is in queue to be put back up on the new pages. (In other words, they didn't drop it, it just hasn't been put back up.) I'll fill you in on what I know, learned by reading an email sent to all HT/HTL program directors about 2 months ago, and what was talked about at educator's forum at the 2005 NSH meeting in Florida. Also, I talked with Dr. Blair Holladay (head of ASCP BOR) when the email first came out, and with Gerri Piscorski (exam manager) (312-541-4887), after that, to get more information. Histotechs were the only category still required to do a practical. Med techs don't have to prove they can make a blood smear or can do a Gram stain or can streak an agar plate. Cytotechs don't have to prove they can make a FNA smear or do a Pap stain. Phlebotomists don't have to prove they can get blood from someone with collapsed veins, or do a heel stick on a newborn.All other categories are tested by just a written exam. The majority of histotechs pass the practical portion. Of those that don't pass the practical, the majority of these also did not pass the written portion. In other words, very few people pass the written but fail the practical. Most either pass the practical/fail the written, or they fail both parts, or they pass both parts. So the written portion of the HT/HTL exam was a better indicator of who would pass/fail both parts, than the practical exam. The cost of the grading of the practical exams was very high - flying histotechs and pathologists into Chicago from all over the country, putting them up in hotels for the weekend, feeding them. The graders were not being extravagent - pizza for lunch, sharing cabs to the grading center, etc. The number of slides was reduced from 15 to 9, in part to reduce the number of graders needed, thus reducing the cost. (By statistically picking the right combination of tissues and stains, 9 slides were giving the same pass/fail rate as the previous 15 slides.) The fee charged to the HT/HTL candidates did not cover the cost of grading the practical exam. Not even when an additional $75 fee was added. So ASCP BOR was losing money with each practical exam. Due to concerns about HIPAA, confidentiality, shipping, etc., many candidates were having difficult times obtaining tissue (especially students in college based histotech programs, that were doing rotations in hospital labs for, say, 2 months). This idea of dropping the practical exam has been around for quite a few years. Reducing the number of slides on the practical from 15 to 9 was a compromise, a stepping stone if you would. The people on the ASCP BOR Histotechnology Exam Committee have been taking pictures of poor staining and sectioning artifacts. These will be incorporated into future written exams as troubleshooting and problem-solving questions. What are the causes and how to correct thick/thin sections, wrinkles, folds, splits, microchatter, etc. So the exam is being re-written to incorporate more staining and sectioning problems. Remember, the ASCP BOR Histology Exam Committee with histotechs very involved with NSH, were involved in this discussion/decision. NSH Board has a representative on the Histology Exam Committee/ASCP Board of Governors, who was involved with this topic. ASCP BOR also talked with program directors of HT/HTL schools about this at the NSH convention, asking their opinion. And this topic has been discussed at various committees that I've been on, for the past several years. So it's been coming, slowly, for somewhere over the last 6 years, maybe closer to 10 years, that I'm aware of. Now, some questions you may be having - What is going to happen to the people who didn't pass the practical last year or the year before (2005, 2004)? Each one has been sent a letter, informing them at they must take and pass the practical in 2006 (this year). If they don't pass the practical this year, then they will not have passed their HT or HTL exam. - What about the people taking the HT/HTL exam for the first time this year (2006), who don't pass? They will be given one more year (2007) to take the "make up" practical, in order to pass their HT/HTL exam. So, for just these few people, they will be allowed to take the practical in 2007. But no one else. - How will I know if someone I'm thinking about hiring can really cut or stain, if they don't have to do a practical exam for ASCP? My suggestion - having them do a "practical exam" as part of their interview with you. Hand them 6 blocks, put them in front of a microtome, and give them 20 minutes to section. Then interview them more (to use up time during the drying), and have them load the H&E stainer. While the slides are staining, have a folder of slides for them to look at - some H&E, some special stains. Ask them to identify the stains or the tissue or what's wrong with a poorly stained slide. As long as you use the same type of tissues in the blocks, and the same stained slides, and have EVERY candidate do the test - this is perfectly acceptable way to assess someone you are interviewing for a position. By this time, the H&Es are done. Have them coverslip them Peggy Wenk From JWeems <@t> sjha.org Sun Aug 28 13:18:35 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sun Aug 28 13:21:50 2011 Subject: [Histonet] RE: Cleanser for blades/instruments In-Reply-To: <3AD1556A3658A240AADF7BD8E805086C0142A640EDF3@CORP-EXVS03.corp.uhsinc.biz> References: <3AD1556A3658A240AADF7BD8E805086C0142A640EDF3@CORP-EXVS03.corp.uhsinc.biz> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408279EA7C6@CHEXCMS10.one.ads.che.org> We have used Weck Instrument Cleaner for years. Works well for us. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeter, Brent Sent: Friday, August 26, 2011 18:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cleanser for blades/instruments Histonetters, Can anyone recommend a good cleanser for soaking scissors/blades/instruments? Thanks in advance - Brent Jeter Anatomic Pathology Supervisor The George Washington University Hospital 202-715-5076 (phone) 202-715-4691 (fax) brent.jeter@gwu-hospital.com UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From madary <@t> verizon.net Sun Aug 28 13:23:41 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Sun Aug 28 13:23:54 2011 Subject: [Histonet] peggy wenk comments on HT/HTL practical Message-ID: <1823936.2512777.1314555821247.JavaMail.root@vms170019> The dropping of the practical was explained so well by Peggy Wenk. I never got into the debate(glad I did not after reading Peggys comments), I would have argued to keep it. That said, indeed it is outdated. The fact that we were the last to do it I guess speaks volumes. I did struggle getting tissues for it for sure. Modern day, modern thinking. Thanks for the information Peggy and of course all you do fo this field. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC From lpwenk <@t> sbcglobal.net Sun Aug 28 18:40:39 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Aug 28 18:40:36 2011 Subject: [Histonet] peggy wenk comments on HT/HTL practical In-Reply-To: <1823936.2512777.1314555821247.JavaMail.root@vms170019> References: <1823936.2512777.1314555821247.JavaMail.root@vms170019> Message-ID: Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. How many people are now using: - automated H&E stainers - automated special stainers, including IHC stainer - automated coverslippers - automated microtomes - disposable blades Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health Systems Royal Oak, MI 48073 The above are my opinions and not those of my institution. -----Original Message----- From: madary@verizon.net Sent: Sunday, August 28, 2011 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peggy wenk comments on HT/HTL practical The dropping of the practical was explained so well by Peggy Wenk. I never got into the debate(glad I did not after reading Peggys comments), I would have argued to keep it. That said, indeed it is outdated. The fact that we were the last to do it I guess speaks volumes. I did struggle getting tissues for it for sure. Modern day, modern thinking. Thanks for the information Peggy and of course all you do fo this field. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Mon Aug 29 04:56:30 2011 From: louise.renton <@t> gmail.com (Louise Renton) Date: Mon Aug 29 04:56:37 2011 Subject: [Histonet] in situ for mRNA - refresher course? Message-ID: Hi all, after more than a decade away from the bench I have been asked to start doing ISH on cryosections for BMPs and TGFbetas (mRNA) . I desperately need some guidance - even an offer to training me. i don't even know waht type of probes have been developed in the interim. I am sure I can get some funding for a couple of days overseas...... Of course, vendors with local representation are welcome to contact me. I look forward to a HUMUNGOUS response regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From b-frederick <@t> northwestern.edu Mon Aug 29 07:35:50 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 29 07:35:55 2011 Subject: [Histonet] peggy wenk comments on HT/HTL practical In-Reply-To: References: <1823936.2512777.1314555821247.JavaMail.root@vms170019> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E13ACBD@evcspmbx3.ads.northwestern.edu> I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Sunday, August 28, 2011 6:41 PM To: madary@verizon.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. How many people are now using: - automated H&E stainers - automated special stainers, including IHC stainer - automated coverslippers - automated microtomes - disposable blades Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health Systems Royal Oak, MI 48073 The above are my opinions and not those of my institution. -----Original Message----- From: madary@verizon.net Sent: Sunday, August 28, 2011 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peggy wenk comments on HT/HTL practical The dropping of the practical was explained so well by Peggy Wenk. I never got into the debate(glad I did not after reading Peggys comments), I would have argued to keep it. That said, indeed it is outdated. The fact that we were the last to do it I guess speaks volumes. I did struggle getting tissues for it for sure. Modern day, modern thinking. Thanks for the information Peggy and of course all you do fo this field. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From an.eerdekens <@t> uzleuven.be Mon Aug 29 07:54:54 2011 From: an.eerdekens <@t> uzleuven.be (An Eerdekens) Date: Mon Aug 29 07:55:00 2011 Subject: [Histonet] placental tissue Message-ID: <09F2BA9A1E06C64D9F090FF0FD0D731772781924D0@EX2007-MBX-2.uz.kuleuven.ac.be> Dear collegues, I want to fixate placental tissue. What is the best solution to use? Formaldehyde 4%? neutral-buffered 10% formal saline? Other? Are there some tips and tricks while working with placental tissue? Thanks for your help. Regards Dr An Eerdekens University Hospitals Leuven Belgium From joelleweaver <@t> hotmail.com Mon Aug 29 08:05:47 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Aug 29 08:05:52 2011 Subject: [Histonet] peggy wenk comments on HT/HTL practical In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E13ACBD@evcspmbx3.ads.northwestern.edu> References: <1823936.2512777.1314555821247.JavaMail.root@vms170019>, , <62C639732D3F274DACED033EBDF6ADAF1E13ACBD@evcspmbx3.ads.northwestern.edu> Message-ID: I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. Joelle Joelle Weaver MAOM, BA, (HTL) ASCP From: b-frederick@northwestern.edu To: lpwenk@sbcglobal.net; madary@verizon.net; histonet@lists.utsouthwestern.edu Date: Mon, 29 Aug 2011 12:35:50 +0000 Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical CC: I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Sunday, August 28, 2011 6:41 PM To: madary@verizon.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. How many people are now using: - automated H&E stainers - automated special stainers, including IHC stainer - automated coverslippers - automated microtomes - disposable blades Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health Systems Royal Oak, MI 48073 The above are my opinions and not those of my institution. -----Original Message----- From: madary@verizon.net Sent: Sunday, August 28, 2011 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peggy wenk comments on HT/HTL practical The dropping of the practical was explained so well by Peggy Wenk. I never got into the debate(glad I did not after reading Peggys comments), I would have argued to keep it. That said, indeed it is outdated. The fact that we were the last to do it I guess speaks volumes. I did struggle getting tissues for it for sure. Modern day, modern thinking. Thanks for the information Peggy and of course all you do fo this field. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Aug 29 08:12:32 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Aug 29 08:12:37 2011 Subject: [Histonet] Returning to Histology-Another Idea Won't Work In-Reply-To: <65225DD0-8177-48E4-A022-D3C8A3D84948@imagesbyhopper.com> References: <1314300719.14987.YahooMailClassic@web30304.mail.mud.yahoo.com>, <0C4105D4F31A489792A55F56E30ED47B@HP2010>, <65225DD0-8177-48E4-A022-D3C8A3D84948@imagesbyhopper.com> Message-ID: With all due respect and regard that should be afforded to the indisputable value of experience ( in time spans of 10 years or more), I would add that I do not see how this could be exchanged for academics.To me they are different animals- both adding something to the knowledge base of the person ( and speaking from my perspective of having both under my belt), and from my experience they are beneficial and useful to me, though in very different ways and different situations. I do not have the routes in front of me, and don't spend the time reviewing them that I formerly did, since I am not teaching at the moment, but I do not recall any experience level for any route stipulating 10 years? Joelle Weaver MAOM, BA, (HTL) ASCP > From: histotech@imagesbyhopper.com > Date: Sat, 27 Aug 2011 20:06:18 -0400 > To: lpwenk@sbcglobal.net > Subject: Re: [Histonet] Returning to Histology-Another Idea Won't Work > CC: histonet@lists.utsouthwestern.edu; araniqkslvr@yahoo.com > > 10 years experience to qualify for taking the exam? Is that an ASCP requirement? It sounds like they might be exchanging experience for the bachelor's degree? Have you checked out the online courses? I believe there are some that offer the bachelor's degree. > > Michelle > > Sent from my iPhone > > On Aug 27, 2011, at 1:45 PM, "Lee & Peggy Wenk" wrote: > > > "It looks like the time limits prohibit me from trying this, since I did it so long ago" > > > > What is "IT"? What is prohibiting you from taking the HTL exam? What does having taken and passed the HT exam have to do with taking the HTL exam? > > > > Sorry, I'm really lost as to what you need, and how to help you. > > > > Peggy A. Wenk, HTL(ASCP)SLS > > Beaumont Hospital > > Royal Oak, MI 48073 > > > > "The above response reflects my opinion, and not the hospital's view." > > > > -----Original Message----- From: Paula > > Sent: Thursday, August 25, 2011 3:31 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Returning to Histology-Another Idea Won't Work > > > > I thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either: > > > > Histotechnologist, HTL(ASCP) > > > > Application Fee: $210 > > > > To be eligible for this examination category, an applicant must > > satisfy the requirements of at least one of the following routes: > > > > Route 1: Baccalaureate degree from a regionally > > accredited college/university with a combination of 30 semester hours > > (45 quarter hours) of biology and chemistry AND successful completion of > > a NAACLS accredited Histotechnician or Histotechnology program within > > the last 5 years; OR > > > > Route 2: Baccalaureate degree from a regionally > > accredited college/university with a combination of 30 semester hours > > (45 quarter hours) of biology and chemistry AND one year full time > > acceptable experience in a histopathology (clinical, veterinary, > > industry or research) laboratory in the U.S., Canada or an accredited > > laboratory* within the last ten years. > > > > *laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.) > > > > Clinical Laboratory Experience > > > > To fulfill the experience requirement for the Histotechnologist > > examination, you must have experience, within the last ten years, in the > > following areas: > > > > Fixation > > Microtomy > > Processing > > Staining Any ideas? I have posted this before, but I keep on trying! > > > > Paula > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Aug 29 08:19:55 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Aug 29 08:19:59 2011 Subject: [Histonet] harrasment for humble histotechs In-Reply-To: <840D06FE-5A74-40F7-AA1F-32751738600F@imagesbyhopper.com> References: <33099009.1060937.1314473156376.JavaMail.root@vznit170132>, <840D06FE-5A74-40F7-AA1F-32751738600F@imagesbyhopper.com> Message-ID: I think that I have worked with some of these people, like I posted previously, if you don't commit the effort to learn and perfect your technique and understanding when you are learning and a student, and try to do a "work around"- short cut, by cheating, IT WILL SHOW at some point. This may not catch up with you that day, or even the next, but in a specific lab or market, people get to know pretty quickly and easily who is really committed to doing quality work , who knows the how and why of what they are doing, and who just shows up for a job... Joelle Weaver MAOM, BA, (HTL) ASCP > From: histotech@imagesbyhopper.com > Date: Sat, 27 Aug 2011 16:50:26 -0400 > To: madary@verizon.net > Subject: Re: [Histonet] harrasment for humble histotechs > CC: histonet@lists.utsouthwestern.edu > > :o). I'm not even sure I could find my results after all these years and 9 moves! > > I *do* remember how picture perfect those slides had to be though. Tiny air bubble=graded down. Folds=forget it. Knife marks=bad. > > I did both the HT and the HTL practicals and now new students don't even have to submit them. I didn't cheat, but over the years I saw students attempting to cheat. If I saw them, I would remind them that they were to work independently, but who knows what they did when I wasn't looking? > > Integrity is what you do when no one is looking. > > > > Sent from my iPhone > > On Aug 27, 2011, at 3:25 PM, madary@verizon.net wrote: > > > > > Gentle harrassment. I encourage you to look at you scores for your > > practical and written exam. Between gigs I am getting my important > > documents together and found my old scores for the HTL, no thte HT, > > however, both parts of the exam were taken by yours truly. Never got > > into the talk of practical vs no practical. I do think it si a good > > idea to keep it. Honest techs will be proud to do a practical. Looking > > at scores made me feel good that the next great job is right around > > the corner. > > > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brian <@t> prometheushealthcare.com Mon Aug 29 08:26:35 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Mon Aug 29 08:26:46 2011 Subject: [Histonet] Moh's Tech Opening in Cleveland Message-ID: <00b701cc664f$49c77640$dd5662c0$@com> We are currently looking for a Moh's Tech brand new graduate or with experience. Position is with a large Dermatology Practice in Cleveland with 6 offices in the Greater Cleveland Area. The position involves some travel between offices as well as working 1-2 days in their Dermatopathology lab. 3-4 days the candidate would be cutting tissue for the same physician mostly. Please contact me today if you might know anyone interested! Thanks! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From dencrowl <@t> MIT.EDU Mon Aug 29 09:30:38 2011 From: dencrowl <@t> MIT.EDU (Denise G Crowley) Date: Mon Aug 29 09:30:43 2011 Subject: [Histonet] troubleshooting Oil Red O Message-ID: <6A9DB28F-BBB2-4406-9C44-29B5F964E662@mit.edu> Hi all, I'm using the Churukian method posted by Gayle Callis a few years ago and I have encountered a strange problem. The lipid stains fine, and the Gill's hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous mounting medium. But within a matter of minutes, the hematoxylin leaches out. I popped off the coverslip and tried again with Mayer's hematoxylin with the same results. I have used Vectashield Hard Set before, in fact I pulled a 5 year old control that still looks great. Does anyone have any ideas? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From 41dmb41 <@t> gmail.com Mon Aug 29 09:42:39 2011 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Mon Aug 29 09:43:05 2011 Subject: [Histonet] troubleshooting Oil Red O In-Reply-To: <6A9DB28F-BBB2-4406-9C44-29B5F964E662@mit.edu> References: <6A9DB28F-BBB2-4406-9C44-29B5F964E662@mit.edu> Message-ID: Could it be a bad lot of the Hard Set? Drew On Mon, Aug 29, 2011 at 10:30, Denise G Crowley wrote: > Hi all, > > I'm using the Churukian method posted by Gayle Callis a few years ago and > I have encountered a strange problem. The lipid stains fine, and the Gill's > hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous > mounting medium. But within a matter of minutes, the hematoxylin leaches > out. I popped off the coverslip and tried again with Mayer's hematoxylin > with the same results. I have used Vectashield Hard Set before, in fact I > pulled a 5 year old control that still looks great. Does anyone have any > ideas? > > Denise Crowley > Histology Facility Manager > Koch Center for Integrative Cancer Research > Massachusetts Institute of Technology > 500 Main St. 76-182 > Cambridge MA 02139 > 617-258-8183 > dencrowl@mit.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pathologylab <@t> ymail.com Mon Aug 29 10:16:09 2011 From: pathologylab <@t> ymail.com (Pathology Lab) Date: Mon Aug 29 10:16:14 2011 Subject: [Histonet] excellent condition used embedding center Message-ID: <1314630969.4737.YahooMailNeo@web121419.mail.ne1.yahoo.com> Im looking for an excellent conditions embbeding center ? Lcda. Mary V. Guerrero,BS, MBA,HtL ? ? ? ? ? ? ? Administradora/Coordinadora General ?Pathology Lab. 55?N. Dr. Basora Edificio M?dico IV?Oficina 206 Mayaguez, Puerto Rico 00680 Tel. 787-834-8202 ?Fax: 787-831-5255 ? ? Sra. Dimary Valent?n Secretaria Area de Facturaci?n Advertencia de Confidencialidad: Esta comunicaci?n, incluyendo los escritos que le acompa?an, es para conocimiento y uso exclusivo del destinatario y esta protegida por la LEY FEDERAL HIPAA Y LEY ESTATAL 194 CONSTITUYENDOLO UN DOCUMENTO LEGAL Y PRIVILEGIADO. Si por error usted lo recibe y no es el destinatario ni a sido autorizado para leer este documento,SE LE ADVIERTE QUE ESTA ESTRICTAMENTE PROHIBIDO FOTOCOPIAR, REVELAR, DISTRIBUIR O TOMAR ACCION ALGUNA SOBRE EL CONTENIDO DE ESTA TRANSMISION ELECTRONICA. ? From mcauliff <@t> umdnj.edu Mon Aug 29 10:23:14 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Aug 29 10:22:36 2011 Subject: [Histonet] troubleshooting Oil Red O In-Reply-To: <6A9DB28F-BBB2-4406-9C44-29B5F964E662@mit.edu> References: <6A9DB28F-BBB2-4406-9C44-29B5F964E662@mit.edu> Message-ID: <4E5BAEE2.6040009@umdnj.edu> It may be that Vector has changed the formulation for Hard Set. I suggest you call them up. I have found their technical support to be excellent. Geoff On 8/29/2011 10:30 AM, Denise G Crowley wrote: > Hi all, > > I'm using the Churukian method posted by Gayle Callis a few years ago and I have encountered a strange problem. The lipid stains fine, and the Gill's hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous mounting medium. But within a matter of minutes, the hematoxylin leaches out. I popped off the coverslip and tried again with Mayer's hematoxylin with the same results. I have used Vectashield Hard Set before, in fact I pulled a 5 year old control that still looks great. Does anyone have any ideas? > > Denise Crowley > Histology Facility Manager > Koch Center for Integrative Cancer Research > Massachusetts Institute of Technology > 500 Main St. 76-182 > Cambridge MA 02139 > 617-258-8183 > dencrowl@mit.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Herrick.James <@t> mayo.edu Mon Aug 29 10:25:46 2011 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Mon Aug 29 10:25:52 2011 Subject: [Histonet] iliac artery attachment to slides? Message-ID: <7267A64D75F58241B577876D8A885631038FE709@msgebe41> Good morning everybody!! I am trying to attach iliac arteries embedded in MMA (methyl methacrylate + dibutyl phthalate + perkadox 16) to gelatin coated slides to allow us to stain them with the Movat's Pentachrome. Unfortunately, I have a real problem getting them to attach - shortly after they are placed into 2-MEA for plastic removal, the section detaches from the slide. I have mounted hundreds of sections to slides before with great success, but have never tried iliac arteries before. When mounting them using the conventional method (a drop of 50% ETOH, roll them onto the slide using a plastic coverslip, clamping the stack and placing it into a 45 - 50 degree Celcius oven for 24 to 48 hours), the sections attach beautifully and do not fall off during deplasticization. The problem is that they are completely consumed with wrinkles around the entire circular area of the artery, rendering them unuseable. I have tried dry mounting (without using 50% ETOH or dH2O), I have tried floating the section onto a slide from a warm ETOH or dH2O solution (in water bath), I have tried using a slide warmer, I have tried a heat gun at low and high temperature settings, etc., etc.. The sections look great using the dry mounting methods (i.e. they don't wrinkle), but they detach from the slide very quickly following submersion in 2-MEA. It appears to me that the wrinkles appear following the introduction of the ETOH or dH2O. If anyone would have any suggestions or comments, it would be greatly appreciated. Thanks again for all of your help. Have a great day!! Jim From mary.helie <@t> yale.edu Mon Aug 29 11:06:18 2011 From: mary.helie <@t> yale.edu (Mary Helie) Date: Mon Aug 29 11:06:27 2011 Subject: [Histonet] lot to lot Message-ID: <4E5BB8FA.5010208@yale.edu> Good Afternoon I would like to get an idea of how many slides people are running for lot to lot antibody and detection testing. For example would a tonsil with good internal negative and positive elements be good enough to validate a new lot of LCA? Thank you Mary From rjbuesa <@t> yahoo.com Mon Aug 29 11:21:01 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 29 11:21:05 2011 Subject: [Histonet] lot to lot In-Reply-To: <4E5BB8FA.5010208@yale.edu> Message-ID: <1314634861.88354.YahooMailClassic@web65703.mail.ac4.yahoo.com> I think?yes! Ren? J. --- On Mon, 8/29/11, Mary Helie wrote: From: Mary Helie Subject: [Histonet] lot to lot To: histonet@lists.utsouthwestern.edu Date: Monday, August 29, 2011, 12:06 PM Good Afternoon I would like to get an idea of how many slides people are running for lot to lot antibody and detection testing. For example would a tonsil with good internal negative and positive elements be good enough to validate a new lot of LCA? Thank you Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Mon Aug 29 11:35:41 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Aug 29 11:35:45 2011 Subject: [Histonet] lot to lot In-Reply-To: <1314634861.88354.YahooMailClassic@web65703.mail.ac4.yahoo.com> References: <4E5BB8FA.5010208@yale.edu> <1314634861.88354.YahooMailClassic@web65703.mail.ac4.yahoo.com> Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106DDAD4@hc-mailboxc1-n3.healthcare.uiowa.edu> Mary: I agree with Rene with one caveat. The block should be one that you have well characterized over multiple runs with the previous lot # (like a tonsil control). While not mandatory, I would not use a different or new block for which you have little information. I prefer to cut successive cuts from the block staining one with the old lot and the other with the new lot. This may be a bit anal but it seems to work best in our hands. Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, August 29, 2011 11:21 AM To: histonet@lists.utsouthwestern.edu; mary.helie@yale.edu Subject: Re: [Histonet] lot to lot I think yes! Ren? J. --- On Mon, 8/29/11, Mary Helie wrote: From: Mary Helie Subject: [Histonet] lot to lot To: histonet@lists.utsouthwestern.edu Date: Monday, August 29, 2011, 12:06 PM Good Afternoon I would like to get an idea of how many slides people are running for lot to lot antibody and detection testing. For example would a tonsil with good internal negative and positive elements be good enough to validate a new lot of LCA? Thank you Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From ploykasek <@t> phenopath.com Mon Aug 29 11:40:26 2011 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Aug 29 11:40:48 2011 Subject: [Histonet] lot to lot In-Reply-To: <4E5BB8FA.5010208@yale.edu> References: <4E5BB8FA.5010208@yale.edu> Message-ID: I know different people do lot to lot checks in various ways. We use concentrated antibodies, and so we like to do a 'sandwich' titer check on the new lot using our usual tissue control tissue. For example, if the last CD20 lot was at 1:100 we would check the new lot at 1:50, 1:100, 1:200 using tonsil tissues & our standard SOPs. I did have 1 inspector tell me they thought that was unnecessary. It's our preference to do 3 titers & assess the maximum performance in one fell swoop. Our detection lot to lot check is completely different. We check 8-10 different antibodies using our standard control tissue with the new detection lot. we use antibodies with a variety of cellular expressions to assess cytoplasmic, membranous & nuclear expression. We make sure to include some of our 'fussy' antibodies. Of course we have SOPs & forms to go along with this. Call me crazy - I prefer to think of it as meticulous & dedicated! We have had a couple of problems with detections over the years. Just my 2 cents. Patti Ann Loykasek HTL, QIHC PhenoPath Laboratories On Aug 29, 2011, at 9:06 AM, Mary Helie wrote: > Good Afternoon > > I would like to get an idea of how many slides people are running for lot to lot antibody and detection testing. For example would a tonsil with good internal negative and positive elements be good enough to validate a new lot of LCA? > > Thank you > Mary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From abilger <@t> wellspan.org Mon Aug 29 12:16:02 2011 From: abilger <@t> wellspan.org (Bilger, Andrea) Date: Mon Aug 29 12:16:40 2011 Subject: [Histonet] Histotech position Message-ID: <6D7752544B308D44A902C0BD0EC7BF5C8721E666@EXCH02.wellspan.org> Histonet members, York Hospital in York Pennsylvania is looking for a histotech to work day shift Monday through Friday with an occasional 5 hours on Saturday. If you are interested, apply at www.wellspan.org or contact: Andrea Bilger Team Leader, Histology York Hospital 1001 South George St. York, Pa. 17405 717-851-5040 CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From mary.helie <@t> yale.edu Mon Aug 29 12:24:00 2011 From: mary.helie <@t> yale.edu (Mary Helie) Date: Mon Aug 29 12:24:08 2011 Subject: [Histonet] Thanks! Message-ID: <4E5BCB30.5050400@yale.edu> Thank you all- this was very helpful. I appreciate it. From Carol.Fields <@t> Northside.com Mon Aug 29 13:04:00 2011 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Mon Aug 29 13:04:07 2011 Subject: [Histonet] Cost of an H&E Message-ID: <731941C266951A47BEF11E5EFAAED9C90B666BFA@nsmvexch01.northside.local> Hi Netters, If anyone has figured the cost of an H&E lately will you pleas share the info with me? It has been awhile since I have done this. My email is below if you do not mind sharing. Thank you in advance. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From TJJ <@t> stowers.org Mon Aug 29 13:18:32 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Aug 29 13:18:37 2011 Subject: [Histonet] Re: iliac artery attachment to slides? Message-ID: <2C40E43D1F7A56408C4463FD245DDDF97E107E28@EXCHMB-02.stowers-institute.org> Hi Jim, sounds like you are having a time of it. I figure Jack Ratliff will chime in as soon as he sees this. In the meantime I will give you the same advice he gave to me. If you are having troubles with tissues adhering, try Haupt's adhesive. You can find recipes on the internet to make it yourself, or you can buy it commercially ready to use (www.dornandhart.com). I have heard from several people who use this consistently with their MMA and they swear by it. Have you tried stretching the sections using a few drops of 50% alcohol and a couple of soft brushes prior to covering in plastic and clamping? It's going to be tough getting circular tissues wrinkle free. I hope others with more experience than me will chime in on this. Best wishes, Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From rjbuesa <@t> yahoo.com Mon Aug 29 14:52:53 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 29 14:52:58 2011 Subject: [Histonet] Cost of an H&E In-Reply-To: <731941C266951A47BEF11E5EFAAED9C90B666BFA@nsmvexch01.northside.local> Message-ID: <1314647573.38427.YahooMailClassic@web65701.mail.ac4.yahoo.com> The information on direct costs is in separate e-mail Ren? J. --- On Mon, 8/29/11, Carol Fields wrote: From: Carol Fields Subject: [Histonet] Cost of an H&E To: histonet@lists.utsouthwestern.edu Date: Monday, August 29, 2011, 2:04 PM Hi Netters, If anyone has figured the cost of an H&E lately will you pleas share the info with me?? It has been awhile since I have done this.? My email is below if you do not mind sharing. Thank you in advance. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Aug 29 16:36:01 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 29 16:35:49 2011 Subject: [Histonet] training techs Message-ID: <885964FCAA9A431AB96C3DDC09E1D9EA@Patsyoffice> Hey Everyone, The third tech I trained in the last 3 years just passed her HTL exam last Friday, that all makes me feel pretty good. I just signed an affiliate agreement to be a clinical training site for the Histology program at UND today, they are sending me another student who will be with me for 2 semesters. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From gayle.callis <@t> bresnan.net Mon Aug 29 16:39:50 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Aug 29 16:40:09 2011 Subject: [Histonet] Charcoal filters for VIP1000 K series Message-ID: <000f01cc6694$300ccd00$90266700$@bresnan.net> There are two charcoal filters (#6160) available for a Sakura Finetek VIP 1000, K series tissue processor. These are free for the lab who can use them. Contact Maria Jerome [mjerome.mtedu@gmail.com] for shipping arrangements. She is not on Histonet. From histotech <@t> imagesbyhopper.com Mon Aug 29 18:40:48 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Aug 29 18:41:00 2011 Subject: [Histonet] training techs In-Reply-To: <885964FCAA9A431AB96C3DDC09E1D9EA@Patsyoffice> References: <885964FCAA9A431AB96C3DDC09E1D9EA@Patsyoffice> Message-ID: <331D692F-049F-4BDD-B526-188536BC0F2F@imagesbyhopper.com> Way to go Patsy!! :o) Sent from my iPhone On Aug 29, 2011, at 5:36 PM, "Patsy Ruegg" wrote: > Hey Everyone, > > > > The third tech I trained in the last 3 years just passed her HTL exam last > Friday, that all makes me feel pretty good. I just signed an affiliate > agreement to be a clinical training site for the Histology program at UND > today, they are sending me another student who will be with me for 2 > semesters. > > > > Regards, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotech <@t> imagesbyhopper.com Mon Aug 29 18:47:50 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Aug 29 18:47:55 2011 Subject: [Histonet] peggy wenk comments on HT/HTL practical In-Reply-To: References: <1823936.2512777.1314555821247.JavaMail.root@vms170019> <62C639732D3F274DACED033EBDF6ADAF1E13ACBD@evcspmbx3.ads.northwestern.edu> Message-ID: <98C4B261-D008-4D53-8DA0-887970D665BD@imagesbyhopper.com> Interestingly, I have never been required to section tissue in a job interview. I have worked at four hospitals, three private labs and one research facility (hubby moved us around a bit!) I just recently hired two techs, directly out of school with no real world experience. I did ask them to cut some slides for me! ;o) Michelle Sent from my iPhone On Aug 29, 2011, at 9:05 AM, joelle weaver wrote: > > > I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. > Joelle > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > From: b-frederick@northwestern.edu > To: lpwenk@sbcglobal.net; madary@verizon.net; histonet@lists.utsouthwestern.edu > Date: Mon, 29 Aug 2011 12:35:50 +0000 > Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical > CC: > > I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. > > As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. > > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > Sent: Sunday, August 28, 2011 6:41 PM > To: madary@verizon.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical > > Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. > > How many people are now using: > - automated H&E stainers > - automated special stainers, including IHC stainer > - automated coverslippers > - automated microtomes > - disposable blades > > Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. > > As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. > No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. > > That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Health Systems > Royal Oak, MI 48073 > > The above are my opinions and not those of my institution. > > -----Original Message----- > From: madary@verizon.net > Sent: Sunday, August 28, 2011 2:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] peggy wenk comments on HT/HTL practical > > > The dropping of the practical was explained so well by Peggy Wenk. I > never got into the debate(glad I did not after reading Peggys > comments), I would have argued to keep it. That said, indeed it is > outdated. The fact that we were the last to do it I guess speaks > volumes. I did struggle getting tissues for it for sure. Modern day, > modern thinking. Thanks for the information Peggy and of course all > you do fo this field. > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wgray19 <@t> sc.rr.com Mon Aug 29 19:15:11 2011 From: wgray19 <@t> sc.rr.com (Jeff and Wanda Gray) Date: Mon Aug 29 19:14:57 2011 Subject: [Histonet] RE: Going for the HTL In-Reply-To: <09.F3.10009.A647A5E4@cdptpa-mxlb.mail.rr.com> References: <09.F3.10009.A647A5E4@cdptpa-mxlb.mail.rr.com> Message-ID: <005401cc66a9$e20e5cc0$a62b1640$@rr.com> To Paula: Who wrote, "thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either:" There is an internet program just "down the road" in Charleston for HTLs: www.musc.edu/histoprogram Our program is NACCLS approved, has start dates in April and Sept. You can also call the director, Karen Brinker Geils @ (843) 792-4013. Take a look, I'll bet you can do it! It took me over 10 years, but I got my BS and passed my HTL just this year! Life got in my way for a while, but I did it, and I know you can too! Wanda Shotsberger Gray HT/HTL (ASCP), QIHC From rsrichmond <@t> gmail.com Mon Aug 29 21:42:43 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Aug 29 21:42:48 2011 Subject: [Histonet] Re: peggy wenk comments on HT/HTL practical Message-ID: I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN From louise.renton <@t> gmail.com Tue Aug 30 04:13:45 2011 From: louise.renton <@t> gmail.com (Louise Renton) Date: Tue Aug 30 04:22:46 2011 Subject: [Histonet] ACD RNAscope Message-ID: Hi all - is anyone using the above system for RNA detection in FFPE tissues - it sounds almost too good to be true? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From j.brinker <@t> att.net Tue Aug 30 05:12:40 2011 From: j.brinker <@t> att.net (Jean Brinker) Date: Tue Aug 30 05:12:42 2011 Subject: [Histonet] supply inventory software Message-ID: <1314699160.14821.YahooMailClassic@web180314.mail.gq1.yahoo.com> Hello, Can?anyone recommend? a business accounting software to primarily track supply inventory/expenses? We are growing Urology practice (about to merge with another) and in need of a means of tracking for the lab internally. I appreciate your response. ? Jean Brinker Pathology Supervisor St. Louis Urological Surgeons St. Peters, MO 63376 From micropathlabs <@t> yahoo.com Tue Aug 30 06:36:21 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Aug 30 06:36:24 2011 Subject: [Histonet] Slides Message-ID: <1314704181.54150.YahooMailNeo@web161704.mail.bf1.yahoo.com> Hi all! We are having issues with the tissue falling off of our slides during routine staining. The tissue primarily lifts from the bottom sections on the slides. We have had our stainer checked and?have checked ourselves, the oven appears to be working properly and the water pressure is fine. I tried two different?slide manufacturers, both silane coated, and are getting the same result.?Not sure where to go at this point except to try a"non-coated" slide. Any suggestions on a manufacturer or vendor?? Thanks in advance. Your help is always appreciated! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From pathologylab <@t> ymail.com Tue Aug 30 08:11:06 2011 From: pathologylab <@t> ymail.com (Pathology Lab) Date: Tue Aug 30 08:11:10 2011 Subject: [Histonet] Looking for sponsors Message-ID: <1314709866.51465.YahooMailNeo@web121413.mail.ne1.yahoo.com> Hi, im organizing a group of conference for the histotechs in Puerto Rico, and i would like to know if any company wants to come and share with us and sponsor the educational activity. Thanks and have a nice day ?!!! ? Lcda. Mary V. Guerrero,BS, MBA,HtL ? ? ? ? ? ? ? Administradora/Coordinadora General ?Pathology Lab.? ? 55?N. Dr. Basora Edificio M?dico IV?Oficina 206 Mayaguez, Puerto Rico 00680 Tel. 787-834-8202 ?Fax: 787-831-5255 Sra. Dimary Valent?n?????????? Sra. Iris Franqui??????????????? Sra. Myrna Gonz?lez ? Facturaci?n???????? Reportes ? ? ?Transcripcion This email may contain confidential health information that is legally privileged.? This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited.? If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . From vanessaorsini <@t> msn.com Tue Aug 30 08:15:08 2011 From: vanessaorsini <@t> msn.com (Vanessa Orsini) Date: Tue Aug 30 08:15:16 2011 Subject: [Histonet] brazilin Message-ID: Hi everyone, I'm Vanessa and i'm quite new in the histology field so i need some help... i'm having problems with a LacZ staining on mouse fresh frozen tissue. I managed to get a nice staining also if I think it's a bit variable but I cannot find a good counterstain!! I tried the nuclear fast red but it's not stable in an aqua-based mounting medium and if I do the acohol row before mounting with pertex the morphology of the sections is damaged and the lacz staining is a bit washed away (a problem if the Bgal expression is low) I then tried with ematoxilin, the staining is stable but it's more difficult to discriminate between the blue of the lacZ and the blue of ematoxilin. I find out that the brazilin is a red ematoxilin so i decided to try this one but once the powder arrived i didn't received any instruction for dilution with it I couldn't find anything on the net. Does someone have experience with brazilin? or do you know some other red counterstaining that I could use? Thanks a lot Vanessa From rgeske_2000 <@t> yahoo.com Tue Aug 30 08:33:07 2011 From: rgeske_2000 <@t> yahoo.com (Rob Geske) Date: Tue Aug 30 08:33:16 2011 Subject: [Histonet] Capillary Gap Slides Message-ID: <1314711187.18519.YahooMailClassic@web39421.mail.mud.yahoo.com> All, ? can anyone suggest a source for all three sizes of capillary gap slides?? Ventana, Fisher, and Cole-Palmer no longer stock them.? thanks in advance. ? rob ? From POWELL_SA <@t> mercer.edu Tue Aug 30 08:36:23 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Aug 30 08:36:31 2011 Subject: [Histonet] Re: peggy wenk comments on HT/HTL practical In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE5D0364@MERCERMAIL.MercerU.local> Not a Geek, just a good teacher. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Monday, August 29, 2011 10:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Aug 30 08:39:48 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Aug 30 08:39:52 2011 Subject: [Histonet] Slides In-Reply-To: <1314704181.54150.YahooMailNeo@web161704.mail.bf1.yahoo.com> References: <1314704181.54150.YahooMailNeo@web161704.mail.bf1.yahoo.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE5D0373@MERCERMAIL.MercerU.local> I use StayOn from Leica(Surgipath) which works really well on non-coated slides. I cut autopsy material, unusually large sections of bloody, dried, etc tissue and they stay on with StayOn. Gee I made a rhyme. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Tuesday, August 30, 2011 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides Hi all! We are having issues with the tissue falling off of our slides during routine staining. The tissue primarily lifts from the bottom sections on the slides. We have had our stainer checked and?have checked ourselves, the oven appears to be working properly and the water pressure is fine. I tried two different?slide manufacturers, both silane coated, and are getting the same result.?Not sure where to go at this point except to try a"non-coated" slide. Any suggestions on a manufacturer or vendor?? Thanks in advance. Your help is always appreciated! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Aug 30 09:19:17 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Aug 30 09:19:25 2011 Subject: [Histonet] Urgent - testing for cobalt in thyroid Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408279EA9AD@CHEXCMS10.one.ads.che.org> Does anyone know what facility would do this? Please answer ASAP.. thanks! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sdysart <@t> mirnarx.com Tue Aug 30 09:29:42 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Tue Aug 30 09:29:46 2011 Subject: [Histonet] peggy wenk comments on HT/HTL practical In-Reply-To: <98C4B261-D008-4D53-8DA0-887970D665BD@imagesbyhopper.com> References: <1823936.2512777.1314555821247.JavaMail.root@vms170019><62C639732D3F274DACED033EBDF6ADAF1E13ACBD@evcspmbx3.ads.northwestern.edu> <98C4B261-D008-4D53-8DA0-887970D665BD@imagesbyhopper.com> Message-ID: I have been required to cut slides at every job interview I have ever been to. I think this is good practice to follow because if you know what you are doing you should be fine and have no worries. If you are a book tech and have no practical experience then you should not be hired over someone who does. I think the practical should come back as part of the exam!! I had to do it...when you cut 900 slides to get 9 that are perfect, you learn a thing or two on how to modify yourself to be better!! Just my two cents =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Monday, August 29, 2011 6:48 PM To: joelle weaver Cc: Histonet; Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical Interestingly, I have never been required to section tissue in a job interview. I have worked at four hospitals, three private labs and one research facility (hubby moved us around a bit!) I just recently hired two techs, directly out of school with no real world experience. I did ask them to cut some slides for me! ;o) Michelle Sent from my iPhone On Aug 29, 2011, at 9:05 AM, joelle weaver wrote: > > > I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. > Joelle > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > From: b-frederick@northwestern.edu > To: lpwenk@sbcglobal.net; madary@verizon.net; histonet@lists.utsouthwestern.edu > Date: Mon, 29 Aug 2011 12:35:50 +0000 > Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical > CC: > > I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. > > As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. > > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > Sent: Sunday, August 28, 2011 6:41 PM > To: madary@verizon.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical > > Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. > > How many people are now using: > - automated H&E stainers > - automated special stainers, including IHC stainer > - automated coverslippers > - automated microtomes > - disposable blades > > Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. > > As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. > No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. > > That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Health Systems > Royal Oak, MI 48073 > > The above are my opinions and not those of my institution. > > -----Original Message----- > From: madary@verizon.net > Sent: Sunday, August 28, 2011 2:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] peggy wenk comments on HT/HTL practical > > > The dropping of the practical was explained so well by Peggy Wenk. I > never got into the debate(glad I did not after reading Peggys > comments), I would have argued to keep it. That said, indeed it is > outdated. The fact that we were the last to do it I guess speaks > volumes. I did struggle getting tissues for it for sure. Modern day, > modern thinking. Thanks for the information Peggy and of course all > you do fo this field. > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Tue Aug 30 09:38:26 2011 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Aug 30 09:38:32 2011 Subject: [Histonet] Slides In-Reply-To: <1314704181.54150.YahooMailNeo@web161704.mail.bf1.yahoo.com> References: <1314704181.54150.YahooMailNeo@web161704.mail.bf1.yahoo.com> Message-ID: Try coating the slides yourself with Vector Labs "Vectabond." Neither I nor any of my graduate students have ever lost a section from a slide coated with "Vectabond." -Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathlabs@yahoo.com] Sent: Tuesday, August 30, 2011 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides Hi all! We are having issues with the tissue falling off of our slides during routine staining. The tissue primarily lifts from the bottom sections on the slides. We have had our stainer checked and have checked ourselves, the oven appears to be working properly and the water pressure is fine. I tried two different slide manufacturers, both silane coated, and are getting the same result. Not sure where to go at this point except to try a"non-coated" slide. Any suggestions on a manufacturer or vendor?? Thanks in advance. Your help is always appreciated! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Tue Aug 30 10:36:36 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Tue Aug 30 10:36:41 2011 Subject: [Histonet] peggy wenk comments on HT/HTL practical In-Reply-To: Message-ID: <130E8991F210424096EFC6F42EA33B2408043949@LSCOEXCH1.lsmaster.lifespan.org> I totally agree Sarah :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sdysart@mirnarx.com Sent: Tuesday, August 30, 2011 10:30 AM To: histotech@imagesbyhopper.com; joelleweaver@hotmail.com Cc: histonet@lists.utsouthwestern.edu; madary@verizon.net Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical I have been required to cut slides at every job interview I have ever been to. I think this is good practice to follow because if you know what you are doing you should be fine and have no worries. If you are a book tech and have no practical experience then you should not be hired over someone who does. I think the practical should come back as part of the exam!! I had to do it...when you cut 900 slides to get 9 that are perfect, you learn a thing or two on how to modify yourself to be better!! Just my two cents =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Monday, August 29, 2011 6:48 PM To: joelle weaver Cc: Histonet; Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical Interestingly, I have never been required to section tissue in a job interview. I have worked at four hospitals, three private labs and one research facility (hubby moved us around a bit!) I just recently hired two techs, directly out of school with no real world experience. I did ask them to cut some slides for me! ;o) Michelle Sent from my iPhone On Aug 29, 2011, at 9:05 AM, joelle weaver wrote: > > > I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. > Joelle > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > From: b-frederick@northwestern.edu > To: lpwenk@sbcglobal.net; madary@verizon.net; histonet@lists.utsouthwestern.edu > Date: Mon, 29 Aug 2011 12:35:50 +0000 > Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical > CC: > > I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. > > As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. > > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > Sent: Sunday, August 28, 2011 6:41 PM > To: madary@verizon.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical > > Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. > > How many people are now using: > - automated H&E stainers > - automated special stainers, including IHC stainer > - automated coverslippers > - automated microtomes > - disposable blades > > Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. > > As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. > No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. > > That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Health Systems > Royal Oak, MI 48073 > > The above are my opinions and not those of my institution. > > -----Original Message----- > From: madary@verizon.net > Sent: Sunday, August 28, 2011 2:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] peggy wenk comments on HT/HTL practical > > > The dropping of the practical was explained so well by Peggy Wenk. I > never got into the debate(glad I did not after reading Peggys > comments), I would have argued to keep it. That said, indeed it is > outdated. The fact that we were the last to do it I guess speaks > volumes. I did struggle getting tissues for it for sure. Modern day, > modern thinking. Thanks for the information Peggy and of course all > you do fo this field. > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Aug 30 10:46:12 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Aug 30 10:46:17 2011 Subject: [Histonet] Re: brazilin Message-ID: Vanessa Orsini asks about brazilin as a red nuclear counterstain, noting that she has some dry powder but no directions for it. Indeed brazilin is structurally very similar to hematoxylin. You might try substituting it for dry hematoxylin, with a Mayer formula - aluminum mordant, iodate oxidant. Alternatively, Anatech offers a proprietary "Brazilliant", a similar preparation, which I would certainly try out myself if I had the chance. I have no commercial connection with Anatech. Bob Richmond Samurai Pathologist Knoxville TN From gu.lang <@t> gmx.at Tue Aug 30 11:09:46 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 30 11:09:54 2011 Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical In-Reply-To: References: Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Aug 30 11:34:28 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Aug 30 11:33:51 2011 Subject: [Histonet] brazilin In-Reply-To: References: Message-ID: <4E5D1114.4090809@umdnj.edu> Hi Vanessa: I tried "Brizilliant" several years ago. I purchased the dry powder since I have been mixing my own stains since the 1970's. I even called the vendor as I was unsure about the identity of some of the ingredients. The results on formalin fixed, paraffin embedded rodent tissues were disappointing, a dull orange not bright red. You might have better results with the pre-mixed stain. Perhaps they wills send you a small sample to try on your material. Geoff On 8/30/2011 9:15 AM, Vanessa Orsini wrote: > Hi everyone, > > I'm Vanessa and i'm quite new in the histology field so i need some help... > > i'm having problems with a LacZ staining on mouse fresh frozen tissue. I managed to get a nice staining also if I think it's a bit variable but I cannot find a good counterstain!! > I tried the nuclear fast red but it's not stable in an aqua-based mounting medium and if I do the acohol row before mounting with pertex the morphology of the sections is damaged and the lacz staining is a bit washed away (a problem if the Bgal expression is low) > I then tried with ematoxilin, the staining is stable but it's more difficult to discriminate between the blue of the lacZ and the blue of ematoxilin. > I find out that the brazilin is a red ematoxilin so i decided to try this one but once the powder arrived i didn't received any instruction for dilution with it I couldn't find anything on the net. > > Does someone have experience with brazilin? > or do you know some other red counterstaining that I could use? > > > Thanks a lot > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From contact <@t> excaliburpathology.com Tue Aug 30 11:56:09 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Aug 30 11:56:14 2011 Subject: [Histonet] brazilin In-Reply-To: <4E5D1114.4090809@umdnj.edu> References: <4E5D1114.4090809@umdnj.edu> Message-ID: <1314723369.76687.YahooMailNeo@web1102.biz.mail.sk1.yahoo.com> Hi, ? when I do Lac Z, I counterstain with nuclear fast red, rinse in water, then use one of the aqueous mounting medias that have replaced Crystal Mount. http://www.emsdiasum.com/microscopy/technical/datasheet/17985-12.aspx ? Place enough to cover the section, drain off the excess, completely air dry, and then you can permanently coverslip with resin. This is also the procedure I use for AEC and even Oil Red O. Crystal Mount forms a thin "plastic" protective film over the section. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Geoff McAuliffe To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 30, 2011 11:34 AM Subject: Re: [Histonet] brazilin Hi Vanessa: I tried "Brizilliant" several years ago. I purchased the dry powder since I have been mixing my own stains since the 1970's. I even called the vendor as I was unsure about the identity of some of the ingredients. The results on formalin fixed, paraffin embedded rodent tissues were disappointing, a dull orange not bright red. You might have better results with the pre-mixed stain. Perhaps they wills send you a small sample to try on your material. Geoff On 8/30/2011 9:15 AM, Vanessa Orsini wrote: > Hi everyone, > > I'm Vanessa and i'm quite new in the histology field so i need some help... > > i'm having problems with a LacZ staining on mouse fresh frozen tissue. I managed to get a nice staining also if I think it's a bit variable but I cannot find a good counterstain!! > I tried the nuclear fast red but it's not stable in an aqua-based mounting medium and if I do the acohol row before mounting with pertex the morphology of the sections is damaged and the lacz staining is a bit washed away (a problem if the Bgal expression is low) > I then tried with ematoxilin, the staining is stable but it's more difficult to discriminate between the blue of the lacZ and the blue of ematoxilin. > I find out that the brazilin is a red ematoxilin so i decided to try this one but once the powder arrived i didn't received any instruction for dilution with it I couldn't find anything on the net. > > Does someone have experience with brazilin? > or do you know some other red counterstaining that I could use? > > > Thanks a lot > > Vanessa > > >? ??? ??? ??? ? ??? ??? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Tue Aug 30 12:08:07 2011 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Tue Aug 30 12:08:10 2011 Subject: [Histonet] Humidity in Histology Lab Message-ID: <1314724087.62759.YahooMailNeo@web161619.mail.bf1.yahoo.com> Hi Histonetters! I am having humidity issues in a new lab in Long Beach Ca. It's 68% humidity inside lab. I have my own a/c system and have tried all settings including the dry setting. I had level down to 52% then it went back up. Is there something I can do to control this? I'm new to this area so don't know if humidity is year round or seasonal. What are you all using or doing if you have this problem? Thank you in advance, Jill Jill Cox, HT ASCP From kmerriam2003 <@t> yahoo.com Tue Aug 30 12:29:04 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Aug 30 12:29:08 2011 Subject: [Histonet] ACD RNAscope In-Reply-To: References: Message-ID: <1314725344.2135.YahooMailNeo@web130110.mail.mud.yahoo.com> I have been trying to get it to work using their actin (and control probes) probe on some routine FFPE cell pellets that I had, and I have not had any luck at all with it.? Truly, the kit is a breeze to use, but I can't get the darn thing to work.? I have had some very modest success with their kit on frozen sections. ? I have shipped them some of my slides and they have agreed to test them in their lab. ? Let me know if you?have any success! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Louise Renton To: Histonet Sent: Tuesday, August 30, 2011 5:13 AM Subject: [Histonet] ACD RNAscope Hi all - is anyone using the above system for RNA detection in FFPE tissues - it sounds almost too good to be true? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Tue Aug 30 12:59:26 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Tue Aug 30 12:59:47 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Message-ID: <4E5CD09E020000A800064EFE@ns.luhcares.org> Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Aug 30 13:06:47 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Aug 30 13:06:54 2011 Subject: [Histonet] Humidity in Histology Lab In-Reply-To: <1314724087.62759.YahooMailNeo@web161619.mail.bf1.yahoo.com> Message-ID: The humidity is not usual for Southern California. It's been a bad few weeks. Jill Cox Sent by: histonet-bounces@lists.utsouthwestern.edu 08/30/2011 10:25 AM Please respond to Jill Cox To "Histonet@Lists. Edu" cc Subject [Histonet] Humidity in Histology Lab Hi Histonetters! I am having humidity issues in a new lab in Long Beach Ca. It's 68% humidity inside lab. I have my own a/c system and have tried all settings including the dry setting. I had level down to 52% then it went back up. Is there something I can do to control this? I'm new to this area so don't know if humidity is year round or seasonal. What are you all using or doing if you have this problem? Thank you in advance, Jill Jill Cox, HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Tue Aug 30 13:23:39 2011 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Aug 30 13:23:44 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick aPin References: <4E5CD09E020000A800064EFE@ns.luhcares.org> Message-ID: <90354A475B420441B2A0396E5008D49692C04B@copc-sbs.COPC.local> Matthew, I am in agreement with you. I appreciate Peggy's comments and understand the wherefore and the why regarding this. Unfortunately, something has been lost. We also serve as a clinical internship site for students coming out of Clover Park in the Seattle area. This is a 9 week stint and a challenge considering all we'd like to expose the student to in such a short time. As you mention in your post: speed, accuracy and quality are paramount and we do our best to prepare folks for the real world. I too, am glad to have taken the practical. Again, it's unfortunate that the practical isn't practical anymore. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick aPin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandra.Harrison3 <@t> va.gov Tue Aug 30 13:30:06 2011 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Tue Aug 30 13:30:54 2011 Subject: [Histonet] gout processing procedure Message-ID: Could someone share their gout processing procedure? The specimen is fresh, not in formalin. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 From sdysart <@t> mirnarx.com Tue Aug 30 13:40:21 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Tue Aug 30 13:40:27 2011 Subject: [Histonet] gout processing procedure In-Reply-To: References: Message-ID: DO NOT let the sample come in contact with water!!! Let them fix in 100% alcohol. You will also need to set up a run cycle on the processor that does not have any water either (ie, no formalin or alcohol gradients). Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, August 30, 2011 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gout processing procedure Could someone share their gout processing procedure? The specimen is fresh, not in formalin. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Aug 30 13:42:17 2011 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Tue Aug 30 13:42:44 2011 Subject: [Histonet] gout processing procedure In-Reply-To: References: Message-ID: The specimen must not come in contact with water during fixation and processing. Fixation: 100% ethanol Processing: 100% etoh- 5 min. Xylene: 2 changes, 30 min. each Paraffin: 3 changes 30 minutes each. Hope this helps! Stacy McLaughlin, HT(ASCP) Lead Histology Technician/Laboratory Safety Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 (413)582-2019 Stacy_McLaughlin@Cooley-Dickinson.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, August 30, 2011 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gout processing procedure Could someone share their gout processing procedure? The specimen is fresh, not in formalin. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Tue Aug 30 14:03:50 2011 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Tue Aug 30 14:03:54 2011 Subject: [Histonet] Shandon Embedding Center Advice--Thank you Message-ID: <4E5D3416.1050300@mclean.harvard.edu> Hi everyone: I want to thank all the laboratories, service companies, equipment manufactures, and company representatives who responded to my questions concerning my Shandon Embedding Center. As it turned out, I had the thermal fuse replaced and now my 23 year old machine is up and running again. However, as a result of this experience, I now have some back-up options for buying a new or refurbished machine or renting/leasing one in the future. I will try to put in for a new one next year for our next grant-cycle. Thanks again, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 From mbrooks <@t> incytepathology.com Tue Aug 30 14:21:53 2011 From: mbrooks <@t> incytepathology.com (Matt Brooks) Date: Tue Aug 30 14:21:56 2011 Subject: [Histonet] Evening Histology Analyst Position Message-ID: <706224670091FE47997AEF88EFADE7CA01FA580E@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello Fellow Histotech, InCyte Pathology is looking to fill a Histotech position on the evening shift. InCyte is a private anatomic pathology laboratory located in Spokane Valley, WA. We have 24 pathologists stationed at multiple hospital locations throughout the Inland Northwest. The specimen volume is medium-high, with about 78,000 blocks processed annually. The Histology department operates 24 hours a day Monday-Saturday. The shift that we need to fill is an evening shift. If you are interested please contact either Heather Major or Emily Clyde in our Human Resource department. They can be reached at hmajor@incytepathology.com, eclyde@incytepathology.com or by calling 509-892-2700 and asking to speak with either one of them. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com From sratliff <@t> brownpathology.com Tue Aug 30 14:36:55 2011 From: sratliff <@t> brownpathology.com (Sellestine Ratliff) Date: Tue Aug 30 14:37:07 2011 Subject: [Histonet] RE: Histonet slide In-Reply-To: References: Message-ID: <94452A2808AC344B98FAAA8DF675D39819CE9C@WINSURGE.brownpathology.local> Hi Sheila, We ran into the problem of tissue not sticking to the slide a few years ago. Our problem was in the water line attached to our stainer. We found out that the city had cut off the water to do some repairs. We disconnected our water and flushed out the stainer line with a 10% bleach solution removing debris that had changed the pH in the water. Our problem went away. Maybe this is something you can try. S.Ratliff HT (ACSP) Brown & Associates Houston, Texas -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, August 30, 2011 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 93, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Histotech position (Bilger, Andrea) 2. Thanks! (Mary Helie) 3. Cost of an H&E (Carol Fields) 4. Re: iliac artery attachment to slides? (Johnson, Teri) 5. Re: Cost of an H&E (Rene J Buesa) 6. training techs (Patsy Ruegg) 7. Charcoal filters for VIP1000 K series (gayle callis) 8. Re: training techs (histotech@imagesbyhopper.com) 9. Re: peggy wenk comments on HT/HTL practical (histotech@imagesbyhopper.com) 10. RE: Going for the HTL (Jeff and Wanda Gray) 11. Re: peggy wenk comments on HT/HTL practical (Bob Richmond) 12. ACD RNAscope (Louise Renton) 13. supply inventory software (Jean Brinker) 14. Slides (Sheila Haas) 15. Looking for sponsors (Pathology Lab) 16. brazilin (Vanessa Orsini) 17. Capillary Gap Slides (Rob Geske) 18. RE: Re: peggy wenk comments on HT/HTL practical (Shirley A. Powell) 19. RE: Slides (Shirley A. Powell) 20. Urgent - testing for cobalt in thyroid (Weems, Joyce) 21. RE: peggy wenk comments on HT/HTL practical (sdysart@mirnarx.com) 22. RE: Slides (Smith, Allen) 23. RE: peggy wenk comments on HT/HTL practical (Heath, Nancy L.) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Aug 2011 13:16:02 -0400 From: "Bilger, Andrea" Subject: [Histonet] Histotech position To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <6D7752544B308D44A902C0BD0EC7BF5C8721E666@EXCH02.wellspan.org> Content-Type: text/plain; charset="US-ASCII" Histonet members, York Hospital in York Pennsylvania is looking for a histotech to work day shift Monday through Friday with an occasional 5 hours on Saturday. If you are interested, apply at www.wellspan.org or contact: Andrea Bilger Team Leader, Histology York Hospital 1001 South George St. York, Pa. 17405 717-851-5040 CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ ------------------------------ Message: 2 Date: Mon, 29 Aug 2011 13:24:00 -0400 From: Mary Helie Subject: [Histonet] Thanks! To: histonet@lists.utsouthwestern.edu Message-ID: <4E5BCB30.5050400@yale.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Thank you all- this was very helpful. I appreciate it. ------------------------------ Message: 3 Date: Mon, 29 Aug 2011 14:04:00 -0400 From: "Carol Fields" Subject: [Histonet] Cost of an H&E To: Message-ID: <731941C266951A47BEF11E5EFAAED9C90B666BFA@nsmvexch01.northside.local> Content-Type: text/plain;charset="US-ASCII" Hi Netters, If anyone has figured the cost of an H&E lately will you pleas share the info with me? It has been awhile since I have done this. My email is below if you do not mind sharing. Thank you in advance. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ Message: 4 Date: Mon, 29 Aug 2011 13:18:32 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: iliac artery attachment to slides? To: Histonet Message-ID: <2C40E43D1F7A56408C4463FD245DDDF97E107E28@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Hi Jim, sounds like you are having a time of it. I figure Jack Ratliff will chime in as soon as he sees this. In the meantime I will give you the same advice he gave to me. If you are having troubles with tissues adhering, try Haupt's adhesive. You can find recipes on the internet to make it yourself, or you can buy it commercially ready to use (www.dornandhart.com). I have heard from several people who use this consistently with their MMA and they swear by it. Have you tried stretching the sections using a few drops of 50% alcohol and a couple of soft brushes prior to covering in plastic and clamping? It's going to be tough getting circular tissues wrinkle free. I hope others with more experience than me will chime in on this. Best wishes, Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO ------------------------------ Message: 5 Date: Mon, 29 Aug 2011 12:52:53 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Cost of an H&E To: histonet@lists.utsouthwestern.edu, Carol Fields Message-ID: <1314647573.38427.YahooMailClassic@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The information on direct costs is in separate e-mail Ren? J. --- On Mon, 8/29/11, Carol Fields wrote: From: Carol Fields Subject: [Histonet] Cost of an H&E To: histonet@lists.utsouthwestern.edu Date: Monday, August 29, 2011, 2:04 PM Hi Netters, If anyone has figured the cost of an H&E lately will you pleas share the info with me?? It has been awhile since I have done this.? My email is below if you do not mind sharing. Thank you in advance. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 29 Aug 2011 15:36:01 -0600 From: "Patsy Ruegg" Subject: [Histonet] training techs To: Message-ID: <885964FCAA9A431AB96C3DDC09E1D9EA@Patsyoffice> Content-Type: text/plain; charset="us-ascii" Hey Everyone, The third tech I trained in the last 3 years just passed her HTL exam last Friday, that all makes me feel pretty good. I just signed an affiliate agreement to be a clinical training site for the Histology program at UND today, they are sending me another student who will be with me for 2 semesters. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 7 Date: Mon, 29 Aug 2011 15:39:50 -0600 From: "gayle callis" Subject: [Histonet] Charcoal filters for VIP1000 K series To: Message-ID: <000f01cc6694$300ccd00$90266700$@bresnan.net> Content-Type: text/plain; charset="us-ascii" There are two charcoal filters (#6160) available for a Sakura Finetek VIP 1000, K series tissue processor. These are free for the lab who can use them. Contact Maria Jerome [mjerome.mtedu@gmail.com] for shipping arrangements. She is not on Histonet. ------------------------------ Message: 8 Date: Mon, 29 Aug 2011 19:40:48 -0400 From: "histotech@imagesbyhopper.com" Subject: Re: [Histonet] training techs To: Patsy Ruegg Cc: "" Message-ID: <331D692F-049F-4BDD-B526-188536BC0F2F@imagesbyhopper.com> Content-Type: text/plain; charset=us-ascii Way to go Patsy!! :o) Sent from my iPhone On Aug 29, 2011, at 5:36 PM, "Patsy Ruegg" wrote: > Hey Everyone, > > > > The third tech I trained in the last 3 years just passed her HTL exam last > Friday, that all makes me feel pretty good. I just signed an affiliate > agreement to be a clinical training site for the Histology program at UND > today, they are sending me another student who will be with me for 2 > semesters. > > > > Regards, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Mon, 29 Aug 2011 19:47:50 -0400 From: "histotech@imagesbyhopper.com" Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical To: joelle weaver Cc: Histonet , "" Message-ID: <98C4B261-D008-4D53-8DA0-887970D665BD@imagesbyhopper.com> Content-Type: text/plain; charset=us-ascii Interestingly, I have never been required to section tissue in a job interview. I have worked at four hospitals, three private labs and one research facility (hubby moved us around a bit!) I just recently hired two techs, directly out of school with no real world experience. I did ask them to cut some slides for me! ;o) Michelle Sent from my iPhone On Aug 29, 2011, at 9:05 AM, joelle weaver wrote: > > > I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. > Joelle > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > From: b-frederick@northwestern.edu > To: lpwenk@sbcglobal.net; madary@verizon.net; histonet@lists.utsouthwestern.edu > Date: Mon, 29 Aug 2011 12:35:50 +0000 > Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical > CC: > > I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. > > As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. > > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > Sent: Sunday, August 28, 2011 6:41 PM > To: madary@verizon.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical > > Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. > > How many people are now using: > - automated H&E stainers > - automated special stainers, including IHC stainer > - automated coverslippers > - automated microtomes > - disposable blades > > Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. > > As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. > No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. > > That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Health Systems > Royal Oak, MI 48073 > > The above are my opinions and not those of my institution. > > -----Original Message----- > From: madary@verizon.net > Sent: Sunday, August 28, 2011 2:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] peggy wenk comments on HT/HTL practical > > > The dropping of the practical was explained so well by Peggy Wenk. I > never got into the debate(glad I did not after reading Peggys > comments), I would have argued to keep it. That said, indeed it is > outdated. The fact that we were the last to do it I guess speaks > volumes. I did struggle getting tissues for it for sure. Modern day, > modern thinking. Thanks for the information Peggy and of course all > you do fo this field. > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Mon, 29 Aug 2011 20:15:11 -0400 From: "Jeff and Wanda Gray" Subject: [Histonet] RE: Going for the HTL To: Message-ID: <005401cc66a9$e20e5cc0$a62b1640$@rr.com> Content-Type: text/plain; charset="US-ASCII" To Paula: Who wrote, "thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either:" There is an internet program just "down the road" in Charleston for HTLs: www.musc.edu/histoprogram Our program is NACCLS approved, has start dates in April and Sept. You can also call the director, Karen Brinker Geils @ (843) 792-4013. Take a look, I'll bet you can do it! It took me over 10 years, but I got my BS and passed my HTL just this year! Life got in my way for a while, but I did it, and I know you can too! Wanda Shotsberger Gray HT/HTL (ASCP), QIHC ------------------------------ Message: 11 Date: Mon, 29 Aug 2011 22:42:43 -0400 From: Bob Richmond Subject: [Histonet] Re: peggy wenk comments on HT/HTL practical To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 12 Date: Tue, 30 Aug 2011 11:13:45 +0200 From: Louise Renton Subject: [Histonet] ACD RNAscope To: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi all - is anyone using the above system for RNA detection in FFPE tissues - it sounds almost too good to be true? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 13 Date: Tue, 30 Aug 2011 03:12:40 -0700 (PDT) From: Jean Brinker Subject: [Histonet] supply inventory software To: histonet@lists.utsouthwestern.edu Message-ID: <1314699160.14821.YahooMailClassic@web180314.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, Can?anyone recommend? a business accounting software to primarily track supply inventory/expenses? We are growing Urology practice (about to merge with another) and in need of a means of tracking for the lab internally. I appreciate your response. ? Jean Brinker Pathology Supervisor St. Louis Urological Surgeons St. Peters, MO 63376 ------------------------------ Message: 14 Date: Tue, 30 Aug 2011 04:36:21 -0700 (PDT) From: Sheila Haas Subject: [Histonet] Slides To: "histonet@lists.utsouthwestern.edu" Message-ID: <1314704181.54150.YahooMailNeo@web161704.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all! We are having issues with the tissue falling off of our slides during routine staining. The tissue primarily lifts from the bottom sections on the slides. We have had our stainer checked and?have checked ourselves, the oven appears to be working properly and the water pressure is fine. I tried two different?slide manufacturers, both silane coated, and are getting the same result.?Not sure where to go at this point except to try a"non-coated" slide. Any suggestions on a manufacturer or vendor?? Thanks in advance. Your help is always appreciated! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ------------------------------ Message: 15 Date: Tue, 30 Aug 2011 06:11:06 -0700 (PDT) From: Pathology Lab Subject: [Histonet] Looking for sponsors To: "histonet@lists.utsouthwestern.edu" Message-ID: <1314709866.51465.YahooMailNeo@web121413.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi, im organizing a group of conference for the histotechs in Puerto Rico, and i would like to know if any company wants to come and share with us and sponsor the educational activity. Thanks and have a nice day ?!!! ? Lcda. Mary V. Guerrero,BS, MBA,HtL ? ? ? ? ? ? ? Administradora/Coordinadora General ?Pathology Lab.? ? 55?N. Dr. Basora Edificio M?dico IV?Oficina 206 Mayaguez, Puerto Rico 00680 Tel. 787-834-8202 ?Fax: 787-831-5255 Sra. Dimary Valent?n?????????? Sra. Iris Franqui??????????????? Sra. Myrna Gonz?lez ? Facturaci?n???????? Reportes ? ? ?Transcripcion This email may contain confidential health information that is legally privileged.? This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited.? If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ------------------------------ Message: 16 Date: Tue, 30 Aug 2011 15:15:08 +0200 From: Vanessa Orsini Subject: [Histonet] brazilin To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi everyone, I'm Vanessa and i'm quite new in the histology field so i need some help... i'm having problems with a LacZ staining on mouse fresh frozen tissue. I managed to get a nice staining also if I think it's a bit variable but I cannot find a good counterstain!! I tried the nuclear fast red but it's not stable in an aqua-based mounting medium and if I do the acohol row before mounting with pertex the morphology of the sections is damaged and the lacz staining is a bit washed away (a problem if the Bgal expression is low) I then tried with ematoxilin, the staining is stable but it's more difficult to discriminate between the blue of the lacZ and the blue of ematoxilin. I find out that the brazilin is a red ematoxilin so i decided to try this one but once the powder arrived i didn't received any instruction for dilution with it I couldn't find anything on the net. Does someone have experience with brazilin? or do you know some other red counterstaining that I could use? Thanks a lot Vanessa ------------------------------ Message: 17 Date: Tue, 30 Aug 2011 06:33:07 -0700 (PDT) From: Rob Geske Subject: [Histonet] Capillary Gap Slides To: histonet@lists.utsouthwestern.edu Message-ID: <1314711187.18519.YahooMailClassic@web39421.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 All, ? can anyone suggest a source for all three sizes of capillary gap slides?? Ventana, Fisher, and Cole-Palmer no longer stock them.? thanks in advance. ? rob ? ------------------------------ Message: 18 Date: Tue, 30 Aug 2011 09:36:23 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] Re: peggy wenk comments on HT/HTL practical To: Bob Richmond , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE5D0364@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" Not a Geek, just a good teacher. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Monday, August 29, 2011 10:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 30 Aug 2011 09:39:48 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] Slides To: Sheila Haas , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE5D0373@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="iso-8859-1" I use StayOn from Leica(Surgipath) which works really well on non-coated slides. I cut autopsy material, unusually large sections of bloody, dried, etc tissue and they stay on with StayOn. Gee I made a rhyme. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Tuesday, August 30, 2011 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides Hi all! We are having issues with the tissue falling off of our slides during routine staining. The tissue primarily lifts from the bottom sections on the slides. We have had our stainer checked and?have checked ourselves, the oven appears to be working properly and the water pressure is fine. I tried two different?slide manufacturers, both silane coated, and are getting the same result.?Not sure where to go at this point except to try a"non-coated" slide. Any suggestions on a manufacturer or vendor?? Thanks in advance. Your help is always appreciated! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 30 Aug 2011 10:19:17 -0400 From: "Weems, Joyce" Subject: [Histonet] Urgent - testing for cobalt in thyroid To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408279EA9AD@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Does anyone know what facility would do this? Please answer ASAP.. thanks! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 21 Date: Tue, 30 Aug 2011 09:29:42 -0500 From: Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical To: , Cc: histonet@lists.utsouthwestern.edu, madary@verizon.net Message-ID: Content-Type: text/plain; charset="us-ascii" I have been required to cut slides at every job interview I have ever been to. I think this is good practice to follow because if you know what you are doing you should be fine and have no worries. If you are a book tech and have no practical experience then you should not be hired over someone who does. I think the practical should come back as part of the exam!! I had to do it...when you cut 900 slides to get 9 that are perfect, you learn a thing or two on how to modify yourself to be better!! Just my two cents =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Monday, August 29, 2011 6:48 PM To: joelle weaver Cc: Histonet; Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical Interestingly, I have never been required to section tissue in a job interview. I have worked at four hospitals, three private labs and one research facility (hubby moved us around a bit!) I just recently hired two techs, directly out of school with no real world experience. I did ask them to cut some slides for me! ;o) Michelle Sent from my iPhone On Aug 29, 2011, at 9:05 AM, joelle weaver wrote: > > > I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. > Joelle > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > From: b-frederick@northwestern.edu > To: lpwenk@sbcglobal.net; madary@verizon.net; histonet@lists.utsouthwestern.edu > Date: Mon, 29 Aug 2011 12:35:50 +0000 > Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical > CC: > > I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. > > As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. > > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > Sent: Sunday, August 28, 2011 6:41 PM > To: madary@verizon.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical > > Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. > > How many people are now using: > - automated H&E stainers > - automated special stainers, including IHC stainer > - automated coverslippers > - automated microtomes > - disposable blades > > Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. > > As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. > No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. > > That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Health Systems > Royal Oak, MI 48073 > > The above are my opinions and not those of my institution. > > -----Original Message----- > From: madary@verizon.net > Sent: Sunday, August 28, 2011 2:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] peggy wenk comments on HT/HTL practical > > > The dropping of the practical was explained so well by Peggy Wenk. I > never got into the debate(glad I did not after reading Peggys > comments), I would have argued to keep it. That said, indeed it is > outdated. The fact that we were the last to do it I guess speaks > volumes. I did struggle getting tissues for it for sure. Modern day, > modern thinking. Thanks for the information Peggy and of course all > you do fo this field. > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 30 Aug 2011 14:38:26 +0000 From: "Smith, Allen" Subject: RE: [Histonet] Slides To: Sheila Haas Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Try coating the slides yourself with Vector Labs "Vectabond." Neither I nor any of my graduate students have ever lost a section from a slide coated with "Vectabond." -Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathlabs@yahoo.com] Sent: Tuesday, August 30, 2011 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides Hi all! We are having issues with the tissue falling off of our slides during routine staining. The tissue primarily lifts from the bottom sections on the slides. We have had our stainer checked and have checked ourselves, the oven appears to be working properly and the water pressure is fine. I tried two different slide manufacturers, both silane coated, and are getting the same result. Not sure where to go at this point except to try a"non-coated" slide. Any suggestions on a manufacturer or vendor?? Thanks in advance. Your help is always appreciated! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Tue, 30 Aug 2011 11:36:36 -0400 From: "Heath, Nancy L." Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical To: , , Cc: histonet@lists.utsouthwestern.edu, madary@verizon.net Message-ID: <130E8991F210424096EFC6F42EA33B2408043949@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" I totally agree Sarah :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sdysart@mirnarx.com Sent: Tuesday, August 30, 2011 10:30 AM To: histotech@imagesbyhopper.com; joelleweaver@hotmail.com Cc: histonet@lists.utsouthwestern.edu; madary@verizon.net Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical I have been required to cut slides at every job interview I have ever been to. I think this is good practice to follow because if you know what you are doing you should be fine and have no worries. If you are a book tech and have no practical experience then you should not be hired over someone who does. I think the practical should come back as part of the exam!! I had to do it...when you cut 900 slides to get 9 that are perfect, you learn a thing or two on how to modify yourself to be better!! Just my two cents =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Monday, August 29, 2011 6:48 PM To: joelle weaver Cc: Histonet; Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical Interestingly, I have never been required to section tissue in a job interview. I have worked at four hospitals, three private labs and one research facility (hubby moved us around a bit!) I just recently hired two techs, directly out of school with no real world experience. I did ask them to cut some slides for me! ;o) Michelle Sent from my iPhone On Aug 29, 2011, at 9:05 AM, joelle weaver wrote: > > > I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. > Joelle > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > From: b-frederick@northwestern.edu > To: lpwenk@sbcglobal.net; madary@verizon.net; histonet@lists.utsouthwestern.edu > Date: Mon, 29 Aug 2011 12:35:50 +0000 > Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical > CC: > > I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. > > As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. > > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > Sent: Sunday, August 28, 2011 6:41 PM > To: madary@verizon.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical > > Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. > > How many people are now using: > - automated H&E stainers > - automated special stainers, including IHC stainer > - automated coverslippers > - automated microtomes > - disposable blades > > Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. > > As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. > No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. > > That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Health Systems > Royal Oak, MI 48073 > > The above are my opinions and not those of my institution. > > -----Original Message----- > From: madary@verizon.net > Sent: Sunday, August 28, 2011 2:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] peggy wenk comments on HT/HTL practical > > > The dropping of the practical was explained so well by Peggy Wenk. I > never got into the debate(glad I did not after reading Peggys > comments), I would have argued to keep it. That said, indeed it is > outdated. The fact that we were the last to do it I guess speaks > volumes. I did struggle getting tissues for it for sure. Modern day, > modern thinking. Thanks for the information Peggy and of course all > you do fo this field. > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 93, Issue 41 **************************************** From turkekul <@t> gmail.com Tue Aug 30 14:45:44 2011 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Tue Aug 30 14:45:47 2011 Subject: [Histonet] Congo Red control slides Message-ID: Hi, I would like to know if anybody is purchasing positive control tissue slides for congo red staining. Can you suggest any vendor and catalog number? Thanks, Mesru Turkekul mskcc.org From PMonfils <@t> Lifespan.org Tue Aug 30 15:16:23 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Aug 30 15:16:33 2011 Subject: [Histonet] HistoFix and frozen sections Message-ID: <4EBFF65383B74D49995298C4976D1D5E088C78BE@LSRIEXCH1.lsmaster.lifespan.org> Does anyone here use the fixative called Histofix? If so, does anyone do frozen sections of tissues fixed in it? Or, has anyone attempted it and found it impossible? Thanks for any input. Paul M. From gonavy2003 <@t> gmail.com Tue Aug 30 15:21:01 2011 From: gonavy2003 <@t> gmail.com (Rick T.) Date: Tue Aug 30 15:21:47 2011 Subject: [Histonet] RE: Humidity in Histology Lab Message-ID: <002401cc6752$57742370$065c6a50$@gmail.com> Jill, When I lived in Missouri humidity was a BIG issue. There are some inexpensive portable dehumidifiers that can be purchased, they have sort of a "thermostat" you can set to maintain a certain level of humidity. They are noisy though, we ran ours max setting at night and turned off during the day. Depends on the lab size as to what type and expense of dehumidifier you will need. Check out this website: http://www.simplydehumidifiers.com/ it's a good start. Also, some of my friends in MO tried plants that reduce humidity, the results are unknown. Check them out at http://www.ehow.com/list_5925724_house-plants-reduce-humidity-levels.html Rick T. From rsrichmond <@t> gmail.com Tue Aug 30 15:53:18 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Aug 30 15:53:23 2011 Subject: [Histonet] Re: gout processing procedure Message-ID: Gouty tissue - containing monosodium urate crystals - should be fixed in absolute alcohol, and processed into xylene and paraffin directly - a rather cumbersome thing to do. Urate crystals, particularly in large deposits (tophi), often survive formalin fixation and routine processing anyway. If any crystalline material is seen on gross examination, it's better to pick it out with a needle, suspend it in a drop of water or alcohol, and look at with a polarizing system that includes a full wave plate (the pee lab microscope usually has one of these, the pathologist usuallly doesn't). If you see the characteristic negatively birefringent crystal needles with this approach, you don't need to do anything else. Bob Richmond Samurai Pathologist Knoxville TN From TNMayer <@t> mdanderson.org Tue Aug 30 15:37:52 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Aug 30 15:56:07 2011 Subject: [Histonet] RE: Humidity in Histology Lab In-Reply-To: References: Message-ID: Jill, Try a dehumidifier set up in the lab. The facilities division of your company should have one or they can purchase it for no more than a couple hundred dollars. Depending on the size of your lab you may need more than one. It has to stay on 24/7 to really make a difference. I had two in a lab where I worked once, and it really helped with the coverslips. Remember to empty them frequently so that they will not shut off. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 1 Date: Tue, 30 Aug 2011 10:08:07 -0700 (PDT) From: Jill Cox Subject: [Histonet] To: "Histonet@Lists. Edu" Message-ID: <1314724087.62759.YahooMailNeo@web161619.mail.bf1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Histonetters! I am having humidity issues in a new lab in Long Beach Ca. It's 68% humidity inside lab. I have my own a/c system and have tried all settings including the dry setting. I had level down to 52% then it went back up. Is there something I can do to control this? I'm new to this area so don't know if humidity is year round or seasonal. What are you all using or doing if you have this problem? Thank you in advance, Jill Jill Cox, HT ASCP ------------------------------ From ratliffjack <@t> hotmail.com Tue Aug 30 15:57:50 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Aug 30 15:57:55 2011 Subject: [Histonet] Re: iliac artery attachment to slides? In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF97E107E28@EXCHMB-02.stowers-institute.org> References: <2C40E43D1F7A56408C4463FD245DDDF97E107E28@EXCHMB-02.stowers-institute.org> Message-ID: Jim, I agree with Teri regarding the Haupt's Adhesive and assume that when you mean a gelatin coating that it is possible that you might already be using this product based upon past conversations. If you are using Haupt's, then I am confused that you are having difficulty with your sections remaining attached to the slide. I am in that group Teri mentioned that swear by Haupt's and it ability to retain sections throughout staining. This then leaves me to question the use of 2-MEA for removal of the resin since you seem to be having problems after this step. Please remember a few things (my opinion and I welcome the challenge from others) when mounting resin sections to gelatin coated slides: #1 - The concentration of gelatin in the coating solution is directly proportional to the adhesive property of the section to the slide. #2 - The concentration of the gelatin in the coating solution is directly proportional to the background staining of certain high molecular weight dyes (i.e. hematoxylin, Aniline blue, etc.). #3 - The adhesive property of the gelatin, attachment of the section to the slide, is proportional to the heat activation of the gelatin during the slide section drying phase (i.e. heating in an oven for a period of time at a certain temperature range). Allow me to briefly expand upon this last statement. The best way that I can explain all of this to people is what I simply call the "tape effect". If you use regular Scotch tape on paper in a room temperature environment, 8 or 9 times out of ten you can safely remove the tape without tearing the paper and without a sticky residue. If you repeat the same procedure in a warmer environment, like say you left it on the paper and stuck it to a window inside your car with all the windows up on a hot summer day, you will be able to remove the tape while it is hot without damage to the paper, but you will leave a very sticky residue on both the paper and the window due to the heat activation of the adhesive. However, if you wait until late evening to perform the same task after the tape has cooled, the adhesion is much stronger and you are highly likely to tear the paper and find it difficult to remove easily from the window. This is how I explain heat activation of the gelatin adhesive and it is for this reason that I use an aluminum slide press as a "heat sink" to help dry ALL slide sections evenly and activate the adhesive properties of the gelatin by increasing the internal heat within the slide chambers. Essentially, you are looking for the correct proportion of adhesive concentration to background staining, all while avoiding the melting of the plastic section covers but still so that all slides dry evenly and so that enough heat is achieved to activate and optimize the adhesive properties of the gelatin (Haupt's Adhesive) coated slides. I then deplastify in warmed xylenes at 50 C, three fresh changes for 30-60 minutes depending upon the overall size of the sections (i.e. small sections on 1x3 slides or large sections on 2x3 slides) and do not lose sections! One last thing to consider is that the geometry of your tissue (circular) is also a contributing factor because of the shrinking and swelling effect in using organic solvents like xylenes to deplastify, ethanol's of decreasing concentration to hydrate the sections, water based solutions for staining and then dehydrating again to coverslip. The density of the tissue then also increases this shrinking and swelling effect. For example, this is not as noticeable with undemineralized bone. So, what do you do? Well, obviously you have to identify where your weaknesses are and then start changing the variables one-by-one until you optimize the method. If it was me I would eliminate the 2-MEA first, then ensure adequate and even drying of the slides as also related to adhesive activation in the oven. If you are still having problems, play with the concentration of the gelatin adhesive and learn to live with the background. There really is a lot of directions to go here, but by no means throw in the towel and give up! Hope I have been helpful and not too confusing. :) Best, Jack Simple concept I know, but think about this, the proper balance of gelatin concentration, heating and cooling during the drying phase, subsequent use of chemicals, and the density properties of the tissue, all contribute to the overall section adhesion throughout staining. > From: TJJ@stowers.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 29 Aug 2011 13:18:32 -0500 > Subject: [Histonet] Re: iliac artery attachment to slides? > > Hi Jim, sounds like you are having a time of it. > > I figure Jack Ratliff will chime in as soon as he sees this. In the meantime I will give you the same advice he gave to me. > > If you are having troubles with tissues adhering, try Haupt's adhesive. You can find recipes on the internet to make it yourself, or you can buy it commercially ready to use (www.dornandhart.com). I have heard from several people who use this consistently with their MMA and they swear by it. Have you tried stretching the sections using a few drops of 50% alcohol and a couple of soft brushes prior to covering in plastic and clamping? It's going to be tough getting circular tissues wrinkle free. I hope others with more experience than me will chime in on this. > > Best wishes, > > Teri Johnson, HT(ASCP)QIHC > Head, Histology and Electron Microscopy > Stowers Institute for Medical Research > Kansas City, MO > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Tue Aug 30 15:59:49 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Aug 30 15:59:53 2011 Subject: [Histonet] Humidity in Histology Lab In-Reply-To: <1314724087.62759.YahooMailNeo@web161619.mail.bf1.yahoo.com> References: <1314724087.62759.YahooMailNeo@web161619.mail.bf1.yahoo.com> Message-ID: Is the humitity causing any problems or is the number just not what you're used to seeing when you look at the readout? On Tue, Aug 30, 2011 at 10:08 AM, Jill Cox wrote: > Hi Histonetters! > I am having humidity issues in a new lab in Long Beach Ca. It's 68% > humidity inside lab. I have my own a/c system and have tried all settings > including the dry setting. I had level down to 52% then it went back up. Is > there something I can do to control this? I'm new to this area so don't know > if humidity is year round or seasonal. What are you all using or doing if > you have this problem? Thank you in advance, Jill > > Jill Cox, HT ASCP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From epeters2 <@t> gmu.edu Tue Aug 30 16:24:50 2011 From: epeters2 <@t> gmu.edu (Esther Peters) Date: Tue Aug 30 16:24:54 2011 Subject: [Histonet] HT positions still available! Message-ID: <4E5D5522.6030800@gmu.edu> I am posting the following position announcements for Dr. Patricia Latham, Department of Pathology, at George Washington University (GWU), in Washington, DC. If you are interested in the positions, please contact her directly: platham@mfa.gwu.edu. Applicants should already be located in the DC area. One position is funded for development of a histopathology core lab in the GW School of Biomedical Sciences. This lab will be expected to provide tissue processing, sections, routine and special stains and immunostaining to meet the needs of researchers at GWU, primarily in the Biomedical sciences, but all departments will be invited to submit tissues. This person will need to work independently to deliver excellent quality results in a timely manner from the get-go. The person should have good managerial skills, since there will be a need to maintain inventory and to keep track of the flow of specimens and charges. Ideally, the person would have an entreprenurial spirit since the success of the lab will determine its future existence. The position is assured for 2 years, but it could grow to full-time, if the lab does well. The second position is not available quite yet (but very soon) and this person must also be a qualified histotechnologist with experience. This is a contract position at half-time to provide a service to a Pharma project - now scheduled through May 2015. The work involved for the contract will be very limited to a select number of routine stains and one immunostain. However, the person will need to be entering data into an audit-trail database and to maintain meticulous records and interdepartmental communications on a limited but international scale. There will be significant time not involved in the contract work for research projects that I intend to pursue. It would be ideal for someone wanting to take an advanced degree or to get involved in biomedical research. This person will be more like a research assistant, except for the contract obligations. Esther C. Peters, Ph.D. George Mason University Fairfax, VA From foreightl <@t> gmail.com Tue Aug 30 16:25:42 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Aug 30 16:25:48 2011 Subject: [Histonet] Re: gout processing procedure In-Reply-To: References: Message-ID: Processing from 100% alcohol is important, but so is the cutting and staining. Don't cut sections and place onto a regular waterbath and expect all of the crystals to survive. Some still will, but we cut and place onto a 100% alcohol bath. I'll tell you though it can be difficult to get good sections on an alcohol bath. Then we stain them using an eosin only protocol, skipping any aqueous steps (like hematoxylin, bluing, clarifier, wash steps, etc). The pathologist looks at them using polarizing filters looking for birefringence. We tried doing the De Galantha silver method, but it was too complicated. Good luck! On Tue, Aug 30, 2011 at 1:53 PM, Bob Richmond wrote: > Gouty tissue - containing monosodium urate crystals - should be fixed > in absolute alcohol, and processed into xylene and paraffin directly - > a rather cumbersome thing to do. > > Urate crystals, particularly in large deposits (tophi), often survive > formalin fixation and routine processing anyway. > > If any crystalline material is seen on gross examination, it's better > to pick it out with a needle, suspend it in a drop of water or > alcohol, and look at with a polarizing system that includes a full > wave plate (the pee lab microscope usually has one of these, the > pathologist usuallly doesn't). If you see the characteristic > negatively birefringent crystal needles with this approach, you don't > need to do anything else. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From puth.floresita <@t> gene.com Tue Aug 30 16:37:04 2011 From: puth.floresita <@t> gene.com (Flossy Puth) Date: Tue Aug 30 16:37:30 2011 Subject: [Histonet] 3D Histech automated TMA master Message-ID: Hi all, I was wondering if anyone has used or are familiar with this equipment for making TMA blocks, I would like to hear feedback. "3D Histech TMA master" automated TMA arrayer sold by Caliper Life Sciences Thanks! From JWeems <@t> sjha.org Tue Aug 30 17:17:40 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Aug 30 17:17:47 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <4E5CD09E020000A800064EFE@ns.luhcares.org> References: <4E5CD09E020000A800064EFE@ns.luhcares.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8AF9C@CHEXCMS10.one.ads.che.org> I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From SteveM <@t> mcclainlab.com Tue Aug 30 17:51:11 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Tue Aug 30 17:38:43 2011 Subject: [Histonet] gout procedure Message-ID: Sandra, Gout is readily identified from Direct smears by examining either unstained, mounted slides or Dif Quik-stained slides mounted under polarized light. If processed, start in 70% alcohol and process to paraffin. Do not let slides sit in water during staining. Some uric acid generally remains even in H&E though far less than is visible on direct smear. If gout is received in formalin (uric acid is even more soluble in formalin fixative than water), one can recover it by drying the fixative on a slide in the oven and mounting unstained or Dif-Quik stain. Steve Steve A. McClain, MD 45 Manor Road Smithtown, NY 11787 631 361 4000 From cdisbrow <@t> msn.com Tue Aug 30 18:56:35 2011 From: cdisbrow <@t> msn.com (Carrie Disbrow) Date: Tue Aug 30 18:56:39 2011 Subject: [Histonet] Congo Red Controls In-Reply-To: References: Message-ID: Hi,A pathologist told me to try medullary thyroid carcinoma cases for Congo Red. Cheaper than purchasing if positive.Good luck! From POWELL_SA <@t> mercer.edu Tue Aug 30 19:31:35 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Aug 30 19:31:53 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8AF9C@CHEXCMS10.one.ads.che.org> References: <4E5CD09E020000A800064EFE@ns.luhcares.org>, <92AD9B20A6C38C4587A9FEBE3A30E1640827A8AF9C@CHEXCMS10.one.ads.che.org> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE3B417E@MERCERMAIL.MercerU.local> I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carmen.M.Garcia <@t> uv.es Wed Aug 31 02:37:57 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Wed Aug 31 02:38:14 2011 Subject: [Histonet] Mice embryo cryocuts In-Reply-To: References: Message-ID: <1492119186carmaga6@uv.es> Good moorning everyone: I am Carmen Garcia from the University of Valencia. I am just starting my PhD. Now I have to obtain mice embryo cryocut and I am having some problems with the morphology... We removed the embryos and put them in sucrose 30% like 5 hours, then embebbed in OCT and put them at -80?C, when we are going to cut them we let the in the cryostat like 2 hours and the cut them. Someone can give me any advise of what can we been doing wrong? Thank you so much. ******************************************************* Carmen Mar?a Garc?a Pascual FIVI/INCLIVA/Facultad de Medicina Universidad de Valencia Departamento de P.O.G (Laboratorios) 96.386.40.48 Avd. Blasco Iba?ez 17 46010, Valencia ******************************************************* From ree3 <@t> leicester.ac.uk Wed Aug 31 04:31:25 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Aug 31 04:33:08 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <4E5CD09E020000A800064EFE@ns.luhcares.org> References: <4E5CD09E020000A800064EFE@ns.luhcares.org> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4EA486773@EXC-MBX3.cfs.le.ac.uk> If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recently graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. Cheers Richard Edwards -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: 30 August 2011 18:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Aug 31 05:25:30 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 31 05:26:53 2011 Subject: [Histonet] Congo Red control slides In-Reply-To: References: Message-ID: <44DFCB94DEDA4701847E41F2DA1B8EE2@JoePC> Greetings Histoland, in a pinch, we've also used mayonnaise. We opened one of the small packets you get with sandwiches and smeared a little of it onto a slide and there you go. Also, if you can obtain a fresh pierce of kidney with Renal Cell carcinoma, that will work also. Joe ----- Original Message ----- From: "mesruh turkekul" To: Sent: Tuesday, August 30, 2011 2:45 PM Subject: [Histonet] Congo Red control slides > Hi, > > I would like to know if anybody is purchasing positive control tissue > slides > for congo red staining. > Can you suggest any vendor and catalog number? > > Thanks, > Mesru Turkekul > mskcc.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Aug 31 08:20:09 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Aug 31 08:20:13 2011 Subject: [Histonet] Mice embryo cryocuts In-Reply-To: <1492119186carmaga6@uv.es> References: <1492119186carmaga6@uv.es> Message-ID: You need to leave the embryos in sucrose waaay longer than 5 hours. I would leave them at least overnight, if not more than that. You can probably take them out of the blocks and resink them in sucrose, if you're desparate. But next time, always sink (ie wash in sucrose) for at least overnight. How old are the mice you're sectioning? Write back if that doesn't help! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Aug 31, 2011 at 3:37 AM, Carmen Maria Garcia Pascual < Carmen.M.Garcia@uv.es> wrote: > > > Good moorning everyone: > > I am Carmen Garcia from the University of Valencia. I am just starting > my PhD. Now I have to obtain mice embryo cryocut and I am having some > problems with the morphology... We removed the embryos and put them in > sucrose 30% like 5 hours, then embebbed in OCT and put them at -80?C, > when we are going to cut them we let the in the cryostat like 2 hours > and the cut them. > Someone can give me any advise of what can we been doing wrong? > Thank you so much. > > ******************************************************* > Carmen Mar?a Garc?a Pascual > FIVI/INCLIVA/Facultad de Medicina > Universidad de Valencia > Departamento de P.O.G (Laboratorios) > 96.386.40.48 > Avd. Blasco Iba?ez 17 > 46010, Valencia > ******************************************************* > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joseph-galbraith <@t> uiowa.edu Wed Aug 31 08:36:04 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Wed Aug 31 08:36:09 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238DE3B417E@MERCERMAIL.MercerU.local> References: <4E5CD09E020000A800064EFE@ns.luhcares.org>, <92AD9B20A6C38C4587A9FEBE3A30E1640827A8AF9C@CHEXCMS10.one.ads.che.org> <9BF995BC0E47744E9673A41486E24EE238DE3B417E@MERCERMAIL.MercerU.local> Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106DDF38@hc-mailboxc1-n3.healthcare.uiowa.edu> Histoland: I have to agree also. Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors. Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills. As a University based hospital we collaborate with area programs to provide their students with a practical rotation. Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation. If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP. The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills. Thanks. Joe Galbraith Univ of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems, Joyce; Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From thomas.crowell <@t> novartis.com Wed Aug 31 08:44:10 2011 From: thomas.crowell <@t> novartis.com (Crowell, Thomas) Date: Wed Aug 31 08:44:23 2011 Subject: [Histonet] Common Fixatives Message-ID: Hi All, I would like to get a list of the most common fixatives that are being used in clinical laboratories, both in the USA as well as globally. This would be for all types of specimens that come through your labs. Any help would be greatly appreciated! Thanks, Tom Thomas Crowell Fellow (Molecular Discovery) Novartis Institutes for BioMedical Research, Inc. 45 Sidney Street Cambridge, MA 02139 USA Phone +1 617 8717460 Fax +1 N.A. thomas.crowell@novartis.com www.novartis.com From Herrick.James <@t> mayo.edu Wed Aug 31 09:04:28 2011 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Wed Aug 31 09:04:32 2011 Subject: FW: [Histonet] iliac artery attachment to slides? Message-ID: <7267A64D75F58241B577876D8A885631038FE71B@msgebe41> Good morning everybody!! I am trying to attach iliac arteries embedded in MMA (methyl methacrylate + dibutyl phthalate + perkadox 16) to gelatin coated slides to allow us to stain them with the Movat's Pentachrome. Unfortunately, I have a real problem getting them to attach - shortly after they are placed into 2-MEA for plastic removal, the section detaches from the slide. I have mounted hundreds of sections to slides before with great success, but have never tried iliac arteries before. When mounting them using the conventional method (a drop of 50% ETOH, roll them onto the slide using a plastic coverslip, clamping the stack and placing it into a 45 - 50 degree Celcius oven for 24 to 48 hours), the sections attach beautifully and do not fall off during deplasticization. The problem with the iliac sections is that they are completely consumed with wrinkles around the entire circular area of the artery, rendering them unuseable. I have tried dry mounting (without using 50% ETOH or dH2O), I have tried floating the section onto a slide from a warm ETOH or dH2O solution (in water bath), I have tried using a slide warmer, I have tried a heat gun at low and high temperature settings, etc., etc.. The sections look great using the dry mounting methods (i.e. they don't wrinkle), but they detach from the slide very quickly following submersion in 2-MEA. It seems to me that the wrinkles appear, following the introduction of the ETOH or dH2O. If anyone would have any suggestions or comments, it would be greatly appreciated. Thanks again for all of your help. Have a great day!! Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Wed Aug 31 09:15:29 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Aug 31 09:15:55 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Message-ID: <4E5DEDA1020000A80006503A@ns.luhcares.org> You might be surprised I too agree with Joyce and Richard. I understand very well that a new graduate will not be up to the skill level of an individual that has been working for a while. What I am surprised about is that this program seemed to teach to pass the test and has left all of the technical skills left to be taught by the persons 1st job. This could lead to several painful experiences for not only the facility but the new HT. Were is the disconnect. If a person is doing the OJT route they need to have at least one year of experience signed-off by a pathologist. If a person goes through a program who is responsible for making sure that the base-skills are there? Is there not some standards that a new graduate should be able to cut/embed so many blocks in an hour? Is it reasonable for a new graduate to take 1.5 hours to cut 5 (uterus, appendix, tonsil) one-cuts? From facing to lifting the slide off the water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut blocks? So Richard is also right how is a new grad to get experiance without that 1st gig? But how much resposiblity should be placed on the 1st gig to train a new HT that is supose to have base skills in lab equipment, cutting, embeding, staining etal? More thoughts.... ciao Matt Lunetta BS HT(ASCP) Edwards, Richard E." ree3@leicester.ac.uk If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recently graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. Cheers Richard Edwards >>> "Shirley A. Powell" 08/30/11 6:32 PM >>> I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is nee ded in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Aug 31 09:29:33 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Aug 31 09:29:37 2011 Subject: [Histonet] Re: Congo Red controls Message-ID: Carrie Disbrow notes the use of sections of human medullary type thyroid carcinoma as an amyloid control. This is what I've usually seen used in recent years. Many but not all medullary thyroid carcinomas contain amyloid. I've seen good results with the right material. A couple of questions I've asked on Histonet and never gotten a reply to: Amyoidosis is quite easily produced in mice. Why isn't this tissue available for control use? Has anyone tried Anatech's "Amyloid Red" as a substitute for Congo Red? Bob Richmond Samurai Pathologist Knoxville TN From mwich <@t> 7thwavelabs.com Wed Aug 31 09:33:51 2011 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Wed Aug 31 09:33:55 2011 Subject: [Histonet] Congo Red control slides References: Message-ID: <62A8156F8071C8439080D626DF8C33A6019AC6A2@wave-mail.7thwave.local> American Mastertech, Cat. #CSA0425P -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mesruh turkekul Sent: Tuesday, August 30, 2011 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Congo Red control slides Hi, I would like to know if anybody is purchasing positive control tissue slides for congo red staining. Can you suggest any vendor and catalog number? Thanks, Mesru Turkekul mskcc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 31 09:45:47 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 31 09:45:52 2011 Subject: [Histonet] Common Fixatives In-Reply-To: Message-ID: <1314801947.98002.YahooMailClassic@web65707.mail.ac4.yahoo.com> Do not sweat, 85% = neutral buffered formalin. The remaining 15% are about 20 other used in such a small number of labs that do not merit consideration. Ren? J. --- On Wed, 8/31/11, Crowell, Thomas wrote: From: Crowell, Thomas Subject: [Histonet] Common Fixatives To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, August 31, 2011, 9:44 AM Hi All, I would like to get a list of the most common fixatives that are being used in clinical laboratories, both in the USA as well as globally.? This would be for all types of specimens that come through your labs. Any help would be greatly appreciated! Thanks, Tom Thomas Crowell Fellow (Molecular Discovery) Novartis Institutes for BioMedical Research, Inc. 45 Sidney Street Cambridge, MA 02139 USA Phone? ? +1? 617 8717460 Fax? ? ? ???+1? N.A. thomas.crowell@novartis.com www.novartis.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 31 09:51:36 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 31 09:51:40 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46106DDF38@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: <1314802296.66564.YahooMailClassic@web65711.mail.ac4.yahoo.com> Regardless of all the reasoning and good intentions, or even "advantages" eliminating the practical part of the ASCP (either HT or HTL) and concentrating in the "theory" only, is like graduating a medical student without doing any hospital rotations. I would not want to be treated by such an MD. If cheating in the exam was "a given" it is because those who were supposed to do the training were not doing their job correctly. I would not want to have a recently "certified" HT(ASCP) in my lab that I would have to train the practical aspects of the trade. I would expect that such a certified HT would be able to work with quality since the beginning. You can never correct "a wrong" by doing another even greater "wrong". Ren? J --- On Wed, 8/31/11, Galbraith, Joe wrote: From: Galbraith, Joe Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin To: "Shirley A. Powell" , "Weems, Joyce" , "Matthew Lunetta" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, August 31, 2011, 9:36 AM Histoland: I have to agree also.? Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors.? Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills.? As a University based hospital we collaborate with area programs to provide their students with a practical rotation.? Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation.? If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP.? The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills.? Thanks. Joe Galbraith Univ of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems, Joyce; Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.? If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.? Please reply to the sender that you have received the message in error, then delete it.? Thank you. ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Aug 31 09:52:36 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Aug 31 09:52:42 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <4E5DEDA1020000A80006503A@ns.luhcares.org> References: <4E5DEDA1020000A80006503A@ns.luhcares.org> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E13C110@evcspmbx3.ads.northwestern.edu> I went through histo school (in 1983-84) and we were taught the skills needed to work in a hospital lab- Many of these came into play when we were preparing our practical. I had to cut at my interview, being a new grad, and once I was hired I had my 3 months probation to have the quality and quantity of the techs already there, most of them had been in the field at least 10 years, so I had a basis and a goal to meet in a short time. I survived and worked there for 15 years and learned how to crank out the work with the needed quality. If trained properly, it should not be a problem. You relearn/adapt your skills to do what is needed in your job- that is the reason I was hired- the hospital wanted a tech easy to retrain to their procedures. Bear in mind, OTJ is no longer a viable route (last I heard) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Wednesday, August 31, 2011 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin You might be surprised I too agree with Joyce and Richard. I understand very well that a new graduate will not be up to the skill level of an individual that has been working for a while. What I am surprised about is that this program seemed to teach to pass the test and has left all of the technical skills left to be taught by the persons 1st job. This could lead to several painful experiences for not only the facility but the new HT. Were is the disconnect. If a person is doing the OJT route they need to have at least one year of experience signed-off by a pathologist. If a person goes through a program who is responsible for making sure that the base-skills are there? Is there not some standards that a new graduate should be able to cut/embed so many blocks in an hour? Is it reasonable for a new graduate to take 1.5 hours to cut 5 (uterus, appendix, tonsil) one-cuts? From facing to lifting the slide off the water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut blocks? So Richard is also right how is a new grad to get experiance without that 1st gig? But how much resposiblity should be placed on the 1st gig to train a new HT that is supose to have base skills in lab equipment, cutting, embeding, staining etal? More thoughts.... ciao Matt Lunetta BS HT(ASCP) Edwards, Richard E." ree3@leicester.ac.uk If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recently graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. Cheers Richard Edwards >>> "Shirley A. Powell" 08/30/11 6:32 PM >>> I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is nee ded in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Aug 31 09:58:27 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Aug 31 09:58:38 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <4E5DEDA1020000A80006503A@ns.luhcares.org> References: <4E5DEDA1020000A80006503A@ns.luhcares.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF15446D@LRGHEXVS1.practice.lrgh.org> I have had one tech finish an online HT course and currently a second person looking at going the online route to her HT. In both cases as their supervisor I have had to sign on as their in house trainer. As a trainer it is my job to make sure that they know how to function well in Histology when they are finished. My first tech was working elsewhere when she started school and receive little support with her studies, one of the main reasons she came to work for me was how appalled I was to hear that she was getting not support. By the time she was finished I would of let her work on any specimen removed from either myself or anyone in my family. We get the HT's that we work to get. As a supervisor and trainer, I am only as good as my staff makes me look. If I can go on vacation and the lab does not miss a beat, then I have done my job correctly. So to everyone that gets a fresh tech who got short changed on their training, don't whine about it, take it as an opportunity to shape them to the HT that you need them to be. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Wednesday, August 31, 2011 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin You might be surprised I too agree with Joyce and Richard. I understand very well that a new graduate will not be up to the skill level of an individual that has been working for a while. What I am surprised about is that this program seemed to teach to pass the test and has left all of the technical skills left to be taught by the persons 1st job. This could lead to several painful experiences for not only the facility but the new HT. Were is the disconnect. If a person is doing the OJT route they need to have at least one year of experience signed-off by a pathologist. If a person goes through a program who is responsible for making sure that the base-skills are there? Is there not some standards that a new graduate should be able to cut/embed so many blocks in an hour? Is it reasonable for a new graduate to take 1.5 hours to cut 5 (uterus, appendix, tonsil) one-cuts? From facing to lifting the slide off the water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut blocks? So Richard is also right how is a new grad to get experiance without that 1st gig? But how much resposiblity should be placed on the 1st gig to train a new HT that is supose to have base skills in lab equipment, cutting, embeding, staining etal? More thoughts.... ciao Matt Lunetta BS HT(ASCP) Edwards, Richard E." ree3@leicester.ac.uk If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recently graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. Cheers Richard Edwards >>> "Shirley A. Powell" 08/30/11 6:32 PM >>> I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is nee ded in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Demaris.Mills <@t> leica-microsystems.com Wed Aug 31 10:00:54 2011 From: Demaris.Mills <@t> leica-microsystems.com (Demaris.Mills@leica-microsystems.com) Date: Wed Aug 31 10:00:57 2011 Subject: [Histonet] AUTO: Mills, Demaris is out of the office. (returning 09/01/2011) Message-ID: I am out of the office until 09/01/2011. I will be out of the office with limited access to email Aug 31 to Sept 2. For urgent matters, please contact Christina Wilson at christina.wilson@leica-microsystems.com or 314-422-7140 Note: This is an automated response to your message "Histonet Digest, Vol 93, Issue 44" sent on 8/31/2011 9:16:07 AM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From thiggins <@t> cddmedical.com Wed Aug 31 10:12:58 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Wed Aug 31 10:13:02 2011 Subject: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical References: <20110831142032.7E2D113B6ED5@barracuda.crvinc.net> Message-ID: <000501cc67f0$77fcf420$e001a8c0@cdd.loc> I have to agree with the fact that students should not be released into the workforce without any practical experience. We team up with a local college (St. Phillips College, San Antonio TX) that produces several students a year and we along with other facilities help in the rotations for students to deal with the practical experience they desperately need. In my opinion, any program that produces students without the basic skill set (embedding and microtomy) along with basics of fixation, special stains, etc. should not be allowed to be a "Approved" program. Again, this is just my opinion. I want to know who approves a program that would send a student into the workforce without the basic skill set, that blows me away!!! Producing students that are not functional is taking the Histology field a step backwards in getting the respect and pay over the years that we have worked so hard to receive in the healthcare world. Wow!! Thanks, Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) From joseph-galbraith <@t> uiowa.edu Wed Aug 31 10:19:26 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Wed Aug 31 10:19:31 2011 Subject: FW: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106DE055@hc-mailboxc1-n3.healthcare.uiowa.edu> -----Original Message----- From: Galbraith, Joe Sent: Wednesday, August 31, 2011 10:13 AM To: 'Heath, Nancy L.'; 'histonet-bounces@lists.utsouthwestern.edu' Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin Nancy: I would encourage our regional and national leaders to bring this issue to the board of governors at the NSH and have the board direct the NSH executives to address ASCP regarding setting standards for the programs that with to provide 'graduates' eligible to take the exam. Perhaps the standards should include a practical rotation in some form. Perhaps a requirement for sitting for the exam should be successful completion of a practical rotation. These are just thoughts. Others may have better ideas. Joe -----Original Message----- From: Heath, Nancy L. [mailto:NHeath@Lifespan.org] Sent: Wednesday, August 31, 2011 9:27 AM To: Galbraith, Joe Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin Totally agree with you Joe! How would we as professionals in the field organize our concerns to NSH and/or ASCP? I would think we would need a large group of histotechs to be heard. Nancy Heath, HT(ASCP) Rhode Island Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Wednesday, August 31, 2011 9:36 AM To: Shirley A. Powell; Weems, Joyce; Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin Histoland: I have to agree also. Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors. Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills. As a University based hospital we collaborate with area programs to provide their students with a practical rotation. Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation. If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP. The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills. Thanks. Joe Galbraith Univ of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems, Joyce; Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From mehlikafaire <@t> hotmail.com Wed Aug 31 10:20:32 2011 From: mehlikafaire <@t> hotmail.com (Mehlika Faire) Date: Wed Aug 31 10:20:37 2011 Subject: [Histonet] Common Fixatives In-Reply-To: <1314801947.98002.YahooMailClassic@web65707.mail.ac4.yahoo.com> References: , <1314801947.98002.YahooMailClassic@web65707.mail.ac4.yahoo.com> Message-ID: Depending on the experiment. Our lab uses 4% PFA for cryosections, Methanol:DMSO (4:1) for whole mount IHC, acetone/4%PFA for cell fixation -Mehlika > Date: Wed, 31 Aug 2011 07:45:47 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; thomas.crowell@novartis.com > Subject: Re: [Histonet] Common Fixatives > CC: > > Do not sweat, 85% = neutral buffered formalin. The remaining 15% are about 20 other used in such a small number of labs that do not merit consideration. > Ren? J. > > --- On Wed, 8/31/11, Crowell, Thomas wrote: > > > From: Crowell, Thomas > Subject: [Histonet] Common Fixatives > To: "histonet@lists.utsouthwestern.edu" > Date: Wednesday, August 31, 2011, 9:44 AM > > > Hi All, > > I would like to get a list of the most common fixatives that are being used in clinical laboratories, both in the USA as well as globally. This would be for all types of specimens that come through your labs. > > Any help would be greatly appreciated! > Thanks, > Tom > > > Thomas Crowell > Fellow (Molecular Discovery) > Novartis Institutes for BioMedical > Research, Inc. > 45 Sidney Street > Cambridge, MA 02139 > USA > > Phone +1 617 8717460 > Fax +1 N.A. > thomas.crowell@novartis.com > www.novartis.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Aug 31 10:21:46 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Aug 31 10:21:57 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <1314802296.66564.YahooMailClassic@web65711.mail.ac4.yahoo.com> Message-ID: Graduating students in histotech programs DO clinical rotations. They have hands-on experience. They are not just learning the theory from books. Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 08/31/2011 07:54 AM To "Shirley A. Powell" , JoyceWeems , Matthew Lunetta , "histonet@lists.utsouthwestern.edu" , JoeGalbraith cc Subject RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Regardless of all the reasoning and good intentions, or even "advantages" eliminating the practical part of the ASCP (either HT or HTL) and concentrating in the "theory" only, is like graduating a medical student without doing any hospital rotations. I would not want to be treated by such an MD. If cheating in the exam was "a given" it is because those who were supposed to do the training were not doing their job correctly. I would not want to have a recently "certified" HT(ASCP) in my lab that I would have to train the practical aspects of the trade. I would expect that such a certified HT would be able to work with quality since the beginning. You can never correct "a wrong" by doing another even greater "wrong". Ren? J --- On Wed, 8/31/11, Galbraith, Joe wrote: From: Galbraith, Joe Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin To: "Shirley A. Powell" , "Weems, Joyce" , "Matthew Lunetta" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, August 31, 2011, 9:36 AM Histoland: I have to agree also. Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors. Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills. As a University based hospital we collaborate with area programs to provide their students with a practical rotation. Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation. If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP. The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills. Thanks. Joe Galbraith Univ of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems, Joyce; Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Wed Aug 31 10:27:06 2011 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Wed Aug 31 10:27:09 2011 Subject: [Histonet] Frozen mouse embryo Message-ID: Hi Carmen, Sometimes people freeze fresh (not fixed) tissues in OCT. When freezing fresh tissues you do not use sucrose. Sucrose is very hyper tonic and will damage live tissue. Alternatively people freeze fixed tissues. Then depending on the stage of the embryo you fix them in neutral buffered formalin or freshly prepared 4% paraformaldehyde for certain time depending on the stage of the embryo. After fixation you wash the fixative with PBS and then place the embryos in 30% sucrose/PBS at 4C. You should keep them in sucrose until they sink to the bottom. If there are air bubbles in the ventricles or other places they will never sink. For embryos bigger than E11.5 days sucrose is overnight at 4C. After sucrose treatment you should incubate the embryos in ice cold OCT for 30 min. Transfer the embryos in fresh ice cold OCT, orient them as you wish and submerge the blocks in iso-pentane (2-methyl butane) pre-chilled in liquid nitrogen to temp of -150C (freezing point of isopentane). Put enough OCT just to cover the embryos and do not keep to long the blocks may crack. Check after 7-10 seconds. It is better to use metal molds lined with aluminium. They conduct heat better. Store the blocks at -80C. Whenever I thaw frozen tissue and freeze it again the morphology is horrible. And with embryos I would not recommend it. Please email me if you need stage specific fixation times. For embryos bigger than E12.5 fix them overnight at room temperature. Best, Mesruh Turkekul mskcc.org From mehlikafaire <@t> hotmail.com Wed Aug 31 10:28:57 2011 From: mehlikafaire <@t> hotmail.com (Mehlika Faire) Date: Wed Aug 31 10:29:01 2011 Subject: [Histonet] Mice embryo cryocuts In-Reply-To: References: , <1492119186carmaga6@uv.es>, Message-ID: Sorry for the repost- forgot to include everyone else. Do you fix the embryos pior to sectioning? or post fix? Usually I place my embryos in 30% sucrose over night in the 4 degree (or until they float to the top-this also depends on the age/size of the embryos) and I fix them in 4% PFA for 1 hr to overnight, depending on the age of the embryo or what the type of treatment for IHC (antigen retrieval w/ trypsin or dnase the embryos requires longer fixation). Then I embed them and section. I haven't had any problems with this method. Hope this helps you out.-Mehlika > Date: Wed, 31 Aug 2011 09:20:09 -0400 > From: talulahgosh@gmail.com > To: Carmen.M.Garcia@uv.es; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Mice embryo cryocuts > CC: > > You need to leave the embryos in sucrose waaay longer than 5 hours. I would > leave them at least overnight, if not more than that. You can probably take > them out of the blocks and resink them in sucrose, if you're desparate. > But next time, always sink (ie wash in sucrose) for at least overnight. How > old are the mice you're sectioning? > Write back if that doesn't help! > > Emily > > A great book should leave you with many experiences, and slightly exhausted. > You should live several lives while reading it. > -William Styron > > > > On Wed, Aug 31, 2011 at 3:37 AM, Carmen Maria Garcia Pascual < > Carmen.M.Garcia@uv.es> wrote: > > > > > > > Good moorning everyone: > > > > I am Carmen Garcia from the University of Valencia. I am just starting > > my PhD. Now I have to obtain mice embryo cryocut and I am having some > > problems with the morphology... We removed the embryos and put them in > > sucrose 30% like 5 hours, then embebbed in OCT and put them at -80?C, > > when we are going to cut them we let the in the cryostat like 2 hours > > and the cut them. > > Someone can give me any advise of what can we been doing wrong? > > Thank you so much. > > > > ******************************************************* > > Carmen Mar?a Garc?a Pascual > > FIVI/INCLIVA/Facultad de Medicina > > Universidad de Valencia > > Departamento de P.O.G (Laboratorios) > > 96.386.40.48 > > Avd. Blasco Iba?ez 17 > > 46010, Valencia > > ******************************************************* > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From candice_camille <@t> yahoo.com Wed Aug 31 10:46:04 2011 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Wed Aug 31 10:46:09 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: References: <1314802296.66564.YahooMailClassic@web65711.mail.ac4.yahoo.com> Message-ID: <1314805564.16696.YahooMailNeo@web125403.mail.ne1.yahoo.com> If my memory severs me correctly.....? is not "required" that a canidate must also have lab experience inorder to take the exam wheather it be from a histotech program or on the job training. So anyone who becomes HT/HTL certified should not only know basic theory but should also have atleast basic experience in micotomy, staining, fixation etc. I thought that this was the case for any of the routes that it takes to become certified. ? What I have found is that a person can start out in a specialty lab and work thier way up ?and get the work experience that is required to sit for the exam however because they only have experience in THAT lab, they are in a disadvantage because they do not have routine experience. ? For example, I know a person who started out in? a research lab and was trained and worked thier way up in about 3 years. They studied and sat for the exam because they had the required work experience and passed. They are now certified. However, whenn they got employed at a hospital that mostly did routines, they did have to be trained a little to undertsand rouine histology. They had never done basic trichromes?and they were?slow at sectioning because speed was not as important where they were from.?But the basic theory and micotmy, he had. ? I say all this to say...... Just because a person has the credientials does not mean that they have all the experience even though they are certified. So sometimes it may take a little training but i admit that it shouldnt take much as if they are a beginner... as they are certified. Thats just my opinion. I remain yours truely, Candice Camille From: Jennifer MacDonald To: Rene J Buesa Cc: "histonet@lists.utsouthwestern.edu" ; Matthew Lunetta ; JoyceWeems ; histonet-bounces@lists.utsouthwestern.edu; Shirley A. Powell Sent: Wednesday, August 31, 2011 10:21 AM Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Graduating students in histotech programs DO clinical rotations.? They have hands-on experience.? They are not just learning the theory from books. Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 08/31/2011 07:54 AM To "Shirley A. Powell" , JoyceWeems , Matthew Lunetta , "histonet@lists.utsouthwestern.edu" , JoeGalbraith cc Subject RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick? ? a Pin Regardless of all the reasoning and good intentions, or even "advantages" eliminating the practical part of the ASCP (either HT or HTL) and concentrating in the "theory" only, is like graduating a medical student without doing any hospital rotations. I would not want to be treated by such an MD. If cheating in the exam was "a given" it is because those who were supposed to do the training were not doing their job correctly. I would not want to have a recently "certified" HT(ASCP) in my lab that I would have to train the practical aspects of the trade. I would expect that such a certified HT would be able to work with quality since the beginning. You can never correct "a wrong" by doing another even greater "wrong". Ren? J --- On Wed, 8/31/11, Galbraith, Joe wrote: From: Galbraith, Joe Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin To: "Shirley A. Powell" , "Weems, Joyce" , "Matthew Lunetta" , "histonet@lists.utsouthwestern.edu" Date: Wednesday, August 31, 2011, 9:36 AM Histoland: I have to agree also.? Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors.? Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills.? As a University based hospital we collaborate with area programs to provide their students with a practical rotation.? Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation.? If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP.? The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills.? Thanks. Joe Galbraith Univ of Iowa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems, Joyce; Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.? If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.? Please reply to the sender that you have received the message in error, then delete it.? Thank you. ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Aug 31 10:58:49 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Aug 31 10:58:53 2011 Subject: [Histonet] LEICA rep for New Mexico? Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFA44@nmdamailsvr.nmda.ad.nmsu.edu> Will this person please contact me? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From ratliffjack <@t> hotmail.com Wed Aug 31 10:59:10 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Aug 31 10:59:15 2011 Subject: [Histonet] iliac artery attachment to slides? In-Reply-To: <7267A64D75F58241B577876D8A885631038FE71B@msgebe41> References: <7267A64D75F58241B577876D8A885631038FE71B@msgebe41> Message-ID: Jim, I think that I might better understand you now from your reply and would like to share my response with others that might be following this thread. From the looks of it you are using 50% EtOH on the slide before rolling out the section. You then mentioned that you once used a drop of xylenes instead of the 50% EtOH and everything works, but you lose sections during deplastification. My routine procedure is to first lubricate the block during cutting with 70% EtOH, I then use 95% EtOH on the slide and periodically (if needed) on section during the process of rolling out the section and orientating on the slide, with the section wet with 95% EtOH I place a section cover over the top, then using bibulous paper and a brayer roller I gently start in the middle rocking back and forth while gradually pressing down with more force until I have attached the section to the slide. This typically help to eliminate wrinkles, however, with your type of specimens you can still have trouble so you need to improvise with the actually pressing method where you actually attach the section to the slide. If you have not tried the higher concentrations of ethanol during the roll out steps, try it and let me know your results. Please keep in mind that I am assuming that you are using something like Haupt's Adhesive (Dorn and Hart Microedge) to coat your slides and that your resin blocks are fairly soft. You see, the higher alcohol concentration acts to help soften the resin so that it is easy to unroll the section and eliminate wrinkles. In fact, the concentration of ethanol and its effectiveness during this step is proportional to the hardness or softness of the resin block. I know that you use MMA + DBP + P-16, but can you tell us the concentrations? I use Acrylosin Infiltration & Embedding solution for my resin work now and the SOFT product contains 10% of the softening component. One other thing to try with the attachment of these difficult type specimens is to float them out on the slide in a pool of 95% EtOH on a slide warmer. The 95% will help you to tease out the wrinkles and the heat of the slide warmer will help to evaporate the ethanol so that you can get some sort of initial attachment to the Haupt's before you try to press the section flat. Also, before you place the section cover on top of the section, add a drop of further diluted Haupt's to the section so that it can dry with the section just in case you are worried about gelatin coating loss. You might get some additional background, but at least it is something that you can tweak if the ethanol steps help to increase your ability to eliminate wrinkles. Jack > Date: Wed, 31 Aug 2011 09:04:28 -0500 > From: Herrick.James@mayo.edu > To: histonet@lists.utsouthwestern.edu > Subject: FW: [Histonet] iliac artery attachment to slides? > > > > Good morning everybody!! > > I am trying to attach iliac arteries embedded in MMA (methyl > methacrylate + dibutyl phthalate + perkadox 16) to gelatin coated slides > to allow us to stain them with the Movat's Pentachrome. Unfortunately, I > have a real problem getting them to attach - shortly after they are > placed into 2-MEA for plastic removal, the section detaches from the > slide. > > I have mounted hundreds of sections to slides before with great success, > but have never tried iliac arteries before. When mounting them using the > conventional method (a drop of 50% ETOH, roll them onto the slide using > a plastic coverslip, clamping the stack and placing it into a 45 - 50 > degree Celcius oven for 24 to 48 hours), the sections attach beautifully > and do not fall off during deplasticization. The problem with the iliac > sections is that they are completely consumed with wrinkles around the > entire circular area of the artery, rendering them unuseable. > > I have tried dry mounting (without using 50% ETOH or dH2O), I have tried > floating the section onto a slide from a warm ETOH or dH2O solution (in > water bath), I have tried using a slide warmer, I have tried a heat gun > at low and high temperature settings, etc., etc.. The sections look > great using the dry mounting methods (i.e. they don't wrinkle), but they > detach from the slide very quickly following submersion in 2-MEA. It > seems to me that the wrinkles appear, following the introduction of the > ETOH or dH2O. > > If anyone would have any suggestions or comments, it would be greatly > appreciated. Thanks again for all of your help. > > Have a great day!! > Jim > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Aug 31 11:01:54 2011 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Aug 31 11:01:59 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <1314805564.16696.YahooMailNeo@web125403.mail.ne1.yahoo.com> Message-ID: <840447922.675966.1314806514331.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I have followed this for a while and was wondering why no one is bringing up the lack of Histology Schools and the small number of graduates they can produce per year as an issue.? Online is fine if you have a lab to work in.? However; one of the people no longer at this facility simply did his book work and an HT then offered himself as just that. He never cut a section or did a stain yet he passed so it is possible. ? Many of us are training OJTs again due to the lack of available people and the in our case the starting salary is low.??It is said it is because?we are not required to complete a BS for an HT only the HTL .? It means we are doing as much as we can to train someone in only one lab with the small amount of time an understaffed Histology Laboratory has yet still have the best training for the field.? We are not recognized fully as Laboratory Professionals yet so it is not really getting any better than it was years ago.? Now we require a 2 year degree for education in science and not much help from the organizations that rule us beyond pay more money.? Many of us are not able to go to meetings ( especially NSH ) due to costs to us personally as the hospitals and Universities are not paying for travel and very little for educational help.? ? Pam Marcum ----- Original Message ----- From: "Candice Smoots " < candice _ camille @yahoo.com> To: " Histonet " < histonet @lists. utsouthwestern . edu > Sent: Wednesday, August 31, 2011 10:46:04 AM Subject: Re: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick????????a Pin If my memory severs me correctly.....? is not "required" that a canidate must also have lab experience inorder to take the exam wheather it be from a histotech program or on the job training. So anyone who becomes HT/ HTL certified should not only know basic theory but should also have atleast basic experience in micotomy , staining, fixation etc. I thought that this was the case for any of the routes that it takes to become certified. ? What I have found is that a person can start out in a specialty lab and work thier way up ?and get the work experience that is required to sit for the exam however because they only have experience in THAT lab, they are in a disadvantage because they do not have routine experience. ? For example, I know a person who started out in? a research lab and was trained and worked thier way up in about 3 years. They studied and sat for the exam because they had the required work experience and passed. They are now certified. However, whenn they got employed at a hospital that mostly did routines, they did have to be trained a little to undertsand rouine histology. They had never done basic trichromes ?and they were?slow at sectioning because speed was not as important where they were from.?But the basic theory and micotmy , he had. ? I say all this to say...... Just because a person has the credientials does not mean that they have all the experience even though they are certified. So sometimes it may take a little training but i admit that it shouldnt take much as if they are a beginner... as they are certified. Thats just my opinion. I remain yours truely , Candice Camille From: Jennifer MacDonald < JMacDonald @ mtsac . edu > To: Rene J Buesa < rjbuesa @yahoo.com> Cc: " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu >; Matthew Lunetta < MLunetta @ luhcares .org>; JoyceWeems < JWeems @ sjha .org>; histonet -bounces@lists. utsouthwestern . edu ; Shirley A. Powell Sent: Wednesday, August 31, 2011 10:21 AM Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin Graduating students in histotech programs DO clinical rotations.? They have hands-on experience.? They are not just learning the theory from books. Rene J Buesa < rjbuesa @yahoo.com> Sent by: histonet -bounces@lists. utsouthwestern . edu 08/31/2011 07:54 AM To "Shirley A. Powell" , JoyceWeems < JWeems @ sjha .org>, Matthew Lunetta < MLunetta @ luhcares .org>, " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu >, JoeGalbraith cc Subject RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick? ? a Pin Regardless of all the reasoning and good intentions, or even "advantages" eliminating the practical part of the ASCP (either HT or HTL ) and concentrating in the "theory" only, is like graduating a medical student without doing any hospital rotations. I would not want to be treated by such an MD. If cheating in the exam was "a given" it is because those who were supposed to do the training were not doing their job correctly. I would not want to have a recently "certified" HT( ASCP ) in my lab that I would have to train the practical aspects of the trade. I would expect that such a certified HT would be able to work with quality since the beginning. You can never correct "a wrong" by doing another even greater "wrong". Ren? J --- On Wed, 8/31/11, Galbraith, Joe wrote: From: Galbraith, Joe Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin To: "Shirley A. Powell" , " Weems , Joyce" < JWeems @ sjha .org>, "Matthew Lunetta " < MLunetta @ luhcares .org>, " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu > Date: Wednesday, August 31, 2011, 9:36 AM Histoland : I have to agree also.? Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors.? Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills.? As a University based hospital we collaborate with area programs to provide their students with a practical rotation.? Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation.? If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP .? The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills.? Thanks. Joe Galbraith Univ of Iowa -----Original Message----- From: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems , Joyce; Matthew Lunetta ; histonet @lists. utsouthwestern . edu Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin I second that Joyce. sp ________________________________________ From: histonet -bounces@lists. utsouthwestern . edu [ histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of Weems , Joyce [ JWeems @ sjha .org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta ; histonet @lists. utsouthwestern . edu Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their " Clinicals ". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet @lists. utsouthwestern . edu Subject: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT ( ASCP ) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: " Gudrun Lang" Subject: AW: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical To: " 'Bob Richmond' " Cc: histonet @lists. utsouthwestern . edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@ dielangs .at> Content-Type: text/plain; charset ="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun ----- Urspr?ngliche Nachricht ----- Von: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] Im Auftrag von Bob Richmond Gesendet : Dienstag , 30. August 2011 04:43 An: histonet @lists. utsouthwestern . edu Betreff : [ Histonet ] Re: peggy wenk comments on HT/ HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura . I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.? If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.? Please reply to the sender that you have received the message in error, then delete it.? Thank you. ________________________________ _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet From POWELL_SA <@t> mercer.edu Wed Aug 31 11:10:13 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Aug 31 11:10:21 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <840447922.675966.1314806514331.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <1314805564.16696.YahooMailNeo@web125403.mail.ne1.yahoo.com> <840447922.675966.1314806514331.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE6E7542@MERCERMAIL.MercerU.local> http://www.naacls.org/docs/Section3_HT.pdf NAACLS spells it out. Online Schools should be following the guidelines. If not they need to rethink their programs if they are approved by NAACLS. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, August 31, 2011 12:02 PM To: Candice Smoots Cc: Histonet Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I have followed this for a while and was wondering why no one is bringing up the lack of Histology Schools and the small number of graduates they can produce per year as an issue. Online is fine if you have a lab to work in. However; one of the people no longer at this facility simply did his book work and an HT then offered himself as just that. He never cut a section or did a stain yet he passed so it is possible. Many of us are training OJTs again due to the lack of available people and the in our case the starting salary is low. It is said it is because we are not required to complete a BS for an HT only the HTL . It means we are doing as much as we can to train someone in only one lab with the small amount of time an understaffed Histology Laboratory has yet still have the best training for the field. We are not recognized fully as Laboratory Professionals yet so it is not really getting any better than it was years ago. Now we require a 2 year degree for education in science and not much help from the organizations that rule us beyond pay more money. Many of us are not able to go to meetings ( especially NSH ) due to costs to us personally as the hospitals and Universities are not paying for travel and very little for educational help. Pam Marcum ----- Original Message ----- From: "Candice Smoots " < candice _ camille @yahoo.com> To: " Histonet " < histonet @lists. utsouthwestern . edu > Sent: Wednesday, August 31, 2011 10:46:04 AM Subject: Re: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin If my memory severs me correctly..... is not "required" that a canidate must also have lab experience inorder to take the exam wheather it be from a histotech program or on the job training. So anyone who becomes HT/ HTL certified should not only know basic theory but should also have atleast basic experience in micotomy , staining, fixation etc. I thought that this was the case for any of the routes that it takes to become certified. What I have found is that a person can start out in a specialty lab and work thier way up and get the work experience that is required to sit for the exam however because they only have experience in THAT lab, they are in a disadvantage because they do not have routine experience. For example, I know a person who started out in a research lab and was trained and worked thier way up in about 3 years. They studied and sat for the exam because they had the required work experience and passed. They are now certified. However, whenn they got employed at a hospital that mostly did routines, they did have to be trained a little to undertsand rouine histology. They had never done basic trichromes and they were slow at sectioning because speed was not as important where they were from. But the basic theory and micotmy , he had. I say all this to say...... Just because a person has the credientials does not mean that they have all the experience even though they are certified. So sometimes it may take a little training but i admit that it shouldnt take much as if they are a beginner... as they are certified. Thats just my opinion. I remain yours truely , Candice Camille From: Jennifer MacDonald < JMacDonald @ mtsac . edu > To: Rene J Buesa < rjbuesa @yahoo.com> Cc: " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu >; Matthew Lunetta < MLunetta @ luhcares .org>; JoyceWeems < JWeems @ sjha .org>; histonet -bounces@lists. utsouthwestern . edu ; Shirley A. Powell Sent: Wednesday, August 31, 2011 10:21 AM Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin Graduating students in histotech programs DO clinical rotations. They have hands-on experience. They are not just learning the theory from books. Rene J Buesa < rjbuesa @yahoo.com> Sent by: histonet -bounces@lists. utsouthwestern . edu 08/31/2011 07:54 AM To "Shirley A. Powell" , JoyceWeems < JWeems @ sjha .org>, Matthew Lunetta < MLunetta @ luhcares .org>, " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu >, JoeGalbraith cc Subject RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin Regardless of all the reasoning and good intentions, or even "advantages" eliminating the practical part of the ASCP (either HT or HTL ) and concentrating in the "theory" only, is like graduating a medical student without doing any hospital rotations. I would not want to be treated by such an MD. If cheating in the exam was "a given" it is because those who were supposed to do the training were not doing their job correctly. I would not want to have a recently "certified" HT( ASCP ) in my lab that I would have to train the practical aspects of the trade. I would expect that such a certified HT would be able to work with quality since the beginning. You can never correct "a wrong" by doing another even greater "wrong". Ren? J --- On Wed, 8/31/11, Galbraith, Joe wrote: From: Galbraith, Joe Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin To: "Shirley A. Powell" , " Weems , Joyce" < JWeems @ sjha .org>, "Matthew Lunetta " < MLunetta @ luhcares .org>, " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu > Date: Wednesday, August 31, 2011, 9:36 AM Histoland : I have to agree also. Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors. Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills. As a University based hospital we collaborate with area programs to provide their students with a practical rotation. Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation. If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP . The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills. Thanks. Joe Galbraith Univ of Iowa -----Original Message----- From: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems , Joyce; Matthew Lunetta ; histonet @lists. utsouthwestern . edu Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin I second that Joyce. sp ________________________________________ From: histonet -bounces@lists. utsouthwestern . edu [ histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of Weems , Joyce [ JWeems @ sjha .org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta ; histonet @lists. utsouthwestern . edu Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their " Clinicals ". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet @lists. utsouthwestern . edu Subject: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT ( ASCP ) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: " Gudrun Lang" Subject: AW: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical To: " 'Bob Richmond' " Cc: histonet @lists. utsouthwestern . edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@ dielangs .at> Content-Type: text/plain; charset ="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun ----- Urspr?ngliche Nachricht ----- Von: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] Im Auftrag von Bob Richmond Gesendet : Dienstag , 30. August 2011 04:43 An: histonet @lists. utsouthwestern . edu Betreff : [ Histonet ] Re: peggy wenk comments on HT/ HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura . I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Aug 31 11:15:05 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Aug 31 11:15:34 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238DE6E7542@MERCERMAIL.MercerU.local> References: <1314805564.16696.YahooMailNeo@web125403.mail.ne1.yahoo.com> <840447922.675966.1314806514331.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> <9BF995BC0E47744E9673A41486E24EE238DE6E7542@MERCERMAIL.MercerU.local> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32030D7E9D@Mail2Node2.ad.uams.edu> I understand the NAACLS rules and live in reality where it is not always followed due to expediency to have a registered HT. It is not the best way to control how we train new Histologists. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, August 31, 2011 11:10 AM To: Pam Marcum; Candice Smoots Cc: Histonet Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin http://www.naacls.org/docs/Section3_HT.pdf NAACLS spells it out. Online Schools should be following the guidelines. If not they need to rethink their programs if they are approved by NAACLS. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, August 31, 2011 12:02 PM To: Candice Smoots Cc: Histonet Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I have followed this for a while and was wondering why no one is bringing up the lack of Histology Schools and the small number of graduates they can produce per year as an issue. Online is fine if you have a lab to work in. However; one of the people no longer at this facility simply did his book work and an HT then offered himself as just that. He never cut a section or did a stain yet he passed so it is possible. Many of us are training OJTs again due to the lack of available people and the in our case the starting salary is low. It is said it is because we are not required to complete a BS for an HT only the HTL . It means we are doing as much as we can to train someone in only one lab with the small amount of time an understaffed Histology Laboratory has yet still have the best training for the field. We are not recognized fully as Laboratory Professionals yet so it is not really getting any better than it was years ago. Now we require a 2 year degree for education in science and not much help from the organizations that rule us beyond pay more money. Many of us are not able to go to meetings ( especially NSH ) due to costs to us personally as the hospitals and Universities are not paying for travel and very little for educational help. Pam Marcum ----- Original Message ----- From: "Candice Smoots " < candice _ camille @yahoo.com> To: " Histonet " < histonet @lists. utsouthwestern . edu > Sent: Wednesday, August 31, 2011 10:46:04 AM Subject: Re: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin If my memory severs me correctly..... is not "required" that a canidate must also have lab experience inorder to take the exam wheather it be from a histotech program or on the job training. So anyone who becomes HT/ HTL certified should not only know basic theory but should also have atleast basic experience in micotomy , staining, fixation etc. I thought that this was the case for any of the routes that it takes to become certified. What I have found is that a person can start out in a specialty lab and work thier way up and get the work experience that is required to sit for the exam however because they only have experience in THAT lab, they are in a disadvantage because they do not have routine experience. For example, I know a person who started out in a research lab and was trained and worked thier way up in about 3 years. They studied and sat for the exam because they had the required work experience and passed. They are now certified. However, whenn they got employed at a hospital that mostly did routines, they did have to be trained a little to undertsand rouine histology. They had never done basic trichromes and they were slow at sectioning because speed was not as important where they were from. But the basic theory and micotmy , he had. I say all this to say...... Just because a person has the credientials does not mean that they have all the experience even though they are certified. So sometimes it may take a little training but i admit that it shouldnt take much as if they are a beginner... as they are certified. Thats just my opinion. I remain yours truely , Candice Camille From: Jennifer MacDonald < JMacDonald @ mtsac . edu > To: Rene J Buesa < rjbuesa @yahoo.com> Cc: " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu >; Matthew Lunetta < MLunetta @ luhcares .org>; JoyceWeems < JWeems @ sjha .org>; histonet -bounces@lists. utsouthwestern . edu ; Shirley A. Powell Sent: Wednesday, August 31, 2011 10:21 AM Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin Graduating students in histotech programs DO clinical rotations. They have hands-on experience. They are not just learning the theory from books. Rene J Buesa < rjbuesa @yahoo.com> Sent by: histonet -bounces@lists. utsouthwestern . edu 08/31/2011 07:54 AM To "Shirley A. Powell" , JoyceWeems < JWeems @ sjha .org>, Matthew Lunetta < MLunetta @ luhcares .org>, " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu >, JoeGalbraith cc Subject RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin Regardless of all the reasoning and good intentions, or even "advantages" eliminating the practical part of the ASCP (either HT or HTL ) and concentrating in the "theory" only, is like graduating a medical student without doing any hospital rotations. I would not want to be treated by such an MD. If cheating in the exam was "a given" it is because those who were supposed to do the training were not doing their job correctly. I would not want to have a recently "certified" HT( ASCP ) in my lab that I would have to train the practical aspects of the trade. I would expect that such a certified HT would be able to work with quality since the beginning. You can never correct "a wrong" by doing another even greater "wrong". Ren? J --- On Wed, 8/31/11, Galbraith, Joe wrote: From: Galbraith, Joe Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin To: "Shirley A. Powell" , " Weems , Joyce" < JWeems @ sjha .org>, "Matthew Lunetta " < MLunetta @ luhcares .org>, " histonet @lists. utsouthwestern . edu " < histonet @lists. utsouthwestern . edu > Date: Wednesday, August 31, 2011, 9:36 AM Histoland : I have to agree also. Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors. Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills. As a University based hospital we collaborate with area programs to provide their students with a practical rotation. Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation. If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP . The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills. Thanks. Joe Galbraith Univ of Iowa -----Original Message----- From: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems , Joyce; Matthew Lunetta ; histonet @lists. utsouthwestern . edu Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin I second that Joyce. sp ________________________________________ From: histonet -bounces@lists. utsouthwestern . edu [ histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of Weems , Joyce [ JWeems @ sjha .org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta ; histonet @lists. utsouthwestern . edu Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their " Clinicals ". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet @lists. utsouthwestern . edu Subject: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT ( ASCP ) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: " Gudrun Lang" Subject: AW: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical To: " 'Bob Richmond' " Cc: histonet @lists. utsouthwestern . edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@ dielangs .at> Content-Type: text/plain; charset ="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun ----- Urspr?ngliche Nachricht ----- Von: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] Im Auftrag von Bob Richmond Gesendet : Dienstag , 30. August 2011 04:43 An: histonet @lists. utsouthwestern . edu Betreff : [ Histonet ] Re: peggy wenk comments on HT/ HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura . I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. 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Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From sbreeden <@t> nmda.nmsu.edu Wed Aug 31 11:17:30 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Aug 31 11:17:33 2011 Subject: [Histonet] New Mexico LEICA rep Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFA46@nmdamailsvr.nmda.ad.nmsu.edu> I am trying to contact my LEICA rep for CONSUMABLES. Thank you to all who've replied so far... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From joelleweaver <@t> hotmail.com Wed Aug 31 11:39:59 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 31 11:40:05 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <4E5DEDA1020000A80006503A@ns.luhcares.org> References: <4E5DEDA1020000A80006503A@ns.luhcares.org> Message-ID: Matt, your first paragraph is exactly correct in my experience with directing a program. And yes, it did produce very unfortunate results in some cases. Almost to the point of negligence, I came to feel in some situations on the part of the school ( test score focus, seemingly little concern if they were actually knowledgable, prepared or safe for themselves and patients) . My pleas for understanding, actual and financial support, fell on "deaf ears"...against mounting budget and funding pressures I reasoned. But all the school focus seemed to be "outcomes" which for them= test scores tied to state money, and NOT actual proficiency or prepartion to work competently in all areas of histology (as it meant for me). It was really disheartening, and I also agree that what I saw were some definate "disconnects" as you have identified (Matt Lunetta's post). Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Wed, 31 Aug 2011 08:15:29 -0600 > From: MLunetta@luhcares.org > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > > You might be surprised I too agree with Joyce and Richard. > > I understand very well that a new graduate will not be up to the skill > level of an individual that has been working for a while. What I am > surprised about is that this program seemed to teach to pass the test > and has left all of the technical skills left to be taught by the > persons 1st job. This could lead to several painful experiences for not > only the facility but the new HT. > > Were is the disconnect. If a person is doing the OJT route they need to > have at least one year of experience signed-off by a pathologist. If a > person goes through a program who is responsible for making sure that > the base-skills are there? Is there not some standards that a new > graduate should be able to cut/embed so many blocks in an hour? Is it > reasonable for a new graduate to take 1.5 hours to cut 5 (uterus, > appendix, tonsil) one-cuts? From facing to lifting the slide off the > water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut > blocks? > > So Richard is also right how is a new grad to get experiance without > that 1st gig? But how much resposiblity should be placed on the 1st gig > to train a new HT that is supose to have base skills in lab equipment, > cutting, embeding, staining etal? > > More thoughts.... > ciao > Matt Lunetta BS HT(ASCP) > > > > > Edwards, Richard E." ree3@leicester.ac.uk > > If you do not employ recently trained individuals, how on earth are they > going to obtain the experience that Matt craves, it's beyond me. My > daughter recently qualified as an Occupational Therapist, when she asked > for the reason that she was unsuccessful in obtaining a post, they said > that as a recently graduated student she had no experience, what > rubbish!!.Thankfully she has now obtained a position under more > enlightened management that is to be found here!. > > Cheers > > Richard Edwards > >>> "Shirley A. Powell" 08/30/11 6:32 PM >>> > I second that Joyce. > sp > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce > [JWeems@sjha.org] > Sent: Tuesday, August 30, 2011 6:17 PM > To: Matthew Lunetta; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To > stick a Pin > > I don't understand how a student of any program would have not a portion > of their program dedicated to these skills. We partner with Darton > College and their students to do a certain number of hours for their > "Clinicals". They know how to do those things, are trained by the > clinical coordinator for the program, and are graded on their work. > > Are they prepared to go into a lab and work like they've done OJT for > 1-2 years? Not at all, but they need to be hired with the understanding > that they will need time and patience to develop their speed and their > skill. > > My 2 cents... > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew > Lunetta > Sent: Tuesday, August 30, 2011 13:59 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To > stick a Pin > > Hey all, > > I found Peggy's comments on why the practical was discontinued to be > very interesting. Of late I have had some experience with a new HT that > graduated from a program and passed the current HT exam. > So, as they say in Great Britain, to stick a pin in the ASCP reasons. > > This new fresh and shiny HT has all the book knowledge we needed them to > have. What they did not have was any technical skills. > 1) never used a microscope or centrifuge. > 2) no special staining experience > 3) no embedding experience > 4) no cutting experience > > When they cut or embed they are no were near the speed, accuracy or > quality that is nee > ded in our industry. While they can answer any > question you ask them they just do not have the technical skills one > would expect from a new graduate. > > I have learned several lessons from this experience. > > 1) I am so very glad I was one of the last HT's to have taken the > practical > 2) Any new HT's will be taking a practical if I am involved in the > selection process. > 3) I will question they quality of any new HT from this particular > program > > While I am sure that there are many new HT's that do have the skills > needed, this one experience has caused me to be more cautious. > > Respectfully, > > Matt Lunetta > BS, HT (ASCP) > > > > > Message: 2 > Date: Tue, 30 Aug 2011 18:09:46 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical > To: "'Bob Richmond'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Dr. Richmond > Here in Austria we have a job open for a pathologist with 5 years > experience. ;) > Please, think it over to come. Lovely mountains, lovely techs... > > It sounds, like you are from that sort of pathologist techs dream of. > Gudrun > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob > Richmond > Gesendet: Dienstag, 30. August 2011 04:43 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical > > I really appreciate Peggy Wenk's analysis of the practical examination > and why it had to be dropped. I never really understood the issue > before. > > I must confess I always enjoyed helping the prospective examinee > obtain exactly the right tissue. > > "No, this endometrium is poorly preserved. We'll arrange with surgery > for a completely fresh specimen - I'll block it initially for the > diagnosis, then we'll fix it overnight and then block it exactly to > specifications. - Ick - this one's been curetted - we'll get another > one". > > "I'll block the margins of this colon resection specimen, then we'll > pin a portion of tissue onto paraffin and fix it flat overnight." > > "Next time I do an autopsy we'll get a lumbar spinal cord in the > intact dura. I'll open the dura dorsally and ventrally with iridectomy > scissors, then we'll hang it in neutral buffered formalin for two > days. Then I'll tie the dura and dependent nerves with a cotton > string. When you embed you'll remove the string, taking care that dura > and nerves remain in position. After that it's all yours. If it > doesn't work the first time, we've got three more levels in the jar." > > OK, I'm a geek, I'm 72 years old, I got a right. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Aug 31 11:41:25 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 31 11:41:29 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101A4EA486773@EXC-MBX3.cfs.le.ac.uk> References: <4E5CD09E020000A800064EFE@ns.luhcares.org>, <7722595275A4DD4FA225B92CDBF174A101A4EA486773@EXC-MBX3.cfs.le.ac.uk> Message-ID: Yes, the old you need experience to get the job, and you need the job to get experience.... Joelle Weaver MAOM, BA, (HTL) ASCP > From: ree3@leicester.ac.uk > To: MLunetta@luhcares.org; histonet@lists.utsouthwestern.edu > Date: Wed, 31 Aug 2011 10:31:25 +0100 > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > CC: > > If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recently graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. > > Cheers > > Richard Edwards > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta > Sent: 30 August 2011 18:59 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > > Hey all, > > I found Peggy's comments on why the practical was discontinued to be > very interesting. Of late I have had some experience with a new HT that > graduated from a program and passed the current HT exam. > So, as they say in Great Britain, to stick a pin in the ASCP reasons. > > This new fresh and shiny HT has all the book knowledge we needed them to > have. What they did not have was any technical skills. > 1) never used a microscope or centrifuge. > 2) no special staining experience > 3) no embedding experience > 4) no cutting experience > > When they cut or embed they are no were near the speed, accuracy or > quality that is needed in our industry. While they can answer any > question you ask them they just do not have the technical skills one > would expect from a new graduate. > > I have learned several lessons from this experience. > > 1) I am so very glad I was one of the last HT's to have taken the > practical > 2) Any new HT's will be taking a practical if I am involved in the > selection process. > 3) I will question they quality of any new HT from this particular > program > > While I am sure that there are many new HT's that do have the skills > needed, this one experience has caused me to be more cautious. > > Respectfully, > > Matt Lunetta > BS, HT (ASCP) > > > > > Message: 2 > Date: Tue, 30 Aug 2011 18:09:46 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical > To: "'Bob Richmond'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Dr. Richmond > Here in Austria we have a job open for a pathologist with 5 years > experience. ;) > Please, think it over to come. Lovely mountains, lovely techs... > > It sounds, like you are from that sort of pathologist techs dream of. > Gudrun > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob > Richmond > Gesendet: Dienstag, 30. August 2011 04:43 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical > > I really appreciate Peggy Wenk's analysis of the practical examination > and why it had to be dropped. I never really understood the issue > before. > > I must confess I always enjoyed helping the prospective examinee > obtain exactly the right tissue. > > "No, this endometrium is poorly preserved. We'll arrange with surgery > for a completely fresh specimen - I'll block it initially for the > diagnosis, then we'll fix it overnight and then block it exactly to > specifications. - Ick - this one's been curetted - we'll get another > one". > > "I'll block the margins of this colon resection specimen, then we'll > pin a portion of tissue onto paraffin and fix it flat overnight." > > "Next time I do an autopsy we'll get a lumbar spinal cord in the > intact dura. I'll open the dura dorsally and ventrally with iridectomy > scissors, then we'll hang it in neutral buffered formalin for two > days. Then I'll tie the dura and dependent nerves with a cotton > string. When you embed you'll remove the string, taking care that dura > and nerves remain in position. After that it's all yours. If it > doesn't work the first time, we've got three more levels in the jar." > > OK, I'm a geek, I'm 72 years old, I got a right. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Wed Aug 31 12:29:49 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Aug 31 12:30:04 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Message-ID: <4E5E1B2D020000A8000650C3@ns.luhcares.org> Tom, I feel that this is very important conversation, not whining about the situation but shining a very bright light on an important subject. I agree that it is an opportunity to help shape a new HT if several factors are in place, the facility can afford the time, cost and personnel that it takes to train. Oh by and by the new HT is coming along and it will take months (much longer than the 3 probationary) to get them to the right place, luckily we can afford (just barley) the time, cost and personnel. At this time 2 months into the process they are not cutting small BX, STATS, embedding of BX, no immuno work and are not to be left alone in the lab. We are hopeful for a good outcome. Matt Lunetta BS HT (ASCP) >>> "Podawiltz, Thomas" 08/31/11 8:59 AM >>> I have had one tech finish an online HT course and currently a second person looking at going the online route to her HT. In both cases as their supervisor I have had to sign on as their in house trainer. As a trainer it is my job to make sure that they know how to function well in Histology when they are finished. My first tech was working elsewhere when she started school and receive little support with her studies, one of the main reasons she came to work for me was how appalled I was to hear that she was getting not support. By the time she was finished I would of let her work on any specimen removed from either myself or anyone in my family. We get the HT's that we work to get. As a supervisor and trainer, I am only as good as my staff makes me look. If I can go on vacation and the lab does not miss a beat, then I have done my job correctly. So to everyone that gets a fresh tech who got short changed on their training, don't whine about it, take it as an opportunity to shape them to the HT that you need them to be. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Wednesday, August 31, 2011 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin You might be surprised I too agree with Joyce and Richard. I understand very well that a new graduate will not be up to the skill level of an individual that has been working for a while. What I am surprised about is that this program seemed to teach to pass the test and has left all of the technical skills left to be taught by the persons 1st job. This could lead to several painful experiences for not only the facility but the new HT. Were is the disconnect. If a person is doing the OJT route they need to have at least one year of experience signed-off by a pathologist. If a person goes through a program who is responsible for making sure that the base-skills are there? Is there not some standards that a new graduate should be able to cut/embed so many blocks in an hour? Is it reasonable for a new graduate to take 1.5 hours to cut 5 (uterus, appendix, tonsil) one-cuts? From facing to lifting the slide off the water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut blocks? So Richard is also right how is a new grad to get experiance without that 1st gig? But how much resposiblity should be placed on the 1st gig to train a new HT that is supose to have base skills in lab equipment, cutting, embeding, staining etal? More thoughts.... ciao Matt Lunetta BS HT(ASCP) Edwards, Richard E." ree3@leicester.ac.uk If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recen tly graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. Cheers Richard Edwards >>> "Shirley A. Powell" 08/30/11 6:32 PM >>> I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is nee ded in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issu e before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From LStadler <@t> cbiolabs.com Wed Aug 31 12:36:06 2011 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Wed Aug 31 12:36:13 2011 Subject: [Histonet] Bar Coding and Sample Tracking Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC0B50E7C4@cbiolabs05.CBiolabs.local> Looking for advice/comments/suggestions regarding sample tracking and data management. I work for a small research institution doing mostly mouse/monkey tissues. Up until now, we have been tracking samples with an excel spreadsheet, but increasing workload is making this more and more difficult. We average probably less than 500 blocks per month (very rough estimate). Basically what I think we need is bar coding for samples and a centralized data storage place so that all the information available for a single sample is in the same place, and can be accessed by anyone anywhere, but not altered (for GLP compliance) as well as capability to expand as our workload varies. This is my first step in looking into this so any input at all is greatly appreciated! Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From talulahgosh <@t> gmail.com Wed Aug 31 12:52:43 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Aug 31 12:52:47 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <4E5E1B2D020000A8000650C3@ns.luhcares.org> References: <4E5E1B2D020000A8000650C3@ns.luhcares.org> Message-ID: How do you become a certified HT and not have any lab experience?! That's crazy. Not that i know anything about being an HT, but I'm a lab tech and I can't imagine going into the job never having been in a lab at all. What exactly do they teach you?! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron From mehlikafaire <@t> hotmail.com Wed Aug 31 12:54:41 2011 From: mehlikafaire <@t> hotmail.com (Mehlika Faire) Date: Wed Aug 31 12:54:46 2011 Subject: [Histonet] pERK staining in PFA Fixed tissues Message-ID: Hello all, I am a Master's student working on staining for pERK in 4% PFA fixed tissues. I came across several protocols where researchers fixed their slides/tissues in PFA and treated their slides/tissues with a methanol gradient (50% to 100% ;dehydration), followed by a ethanol gradient(100% to aqueous). I know people have done this for paraffin fixed tissues, but I am not sure why it is necessary for PFA fixed tissues. Does anyone know the principle behind these steps for pERK staining? Or anyone have a reliable protocol of pERK in PFA fixed sections? Thank you in advance Mehlika From thiggins <@t> cddmedical.com Wed Aug 31 13:07:21 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Wed Aug 31 13:07:26 2011 Subject: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical References: <20110831170305.23A3E13C37D5@barracuda.crvinc.net> Message-ID: <001801cc6808$d4aa0100$e001a8c0@cdd.loc> How would the expression, "you need experience to get the job, and you need the job to get experience" work if a person went through flight school to be a pilot but did not receive any actual flight training but was then allowed to get a job as a pilot flying a commuter plane in charge of peoples lives. Maybe actual training is not as big a deal if you are flipping burgers but Histology is slightly more important than that. At least I feel it is, obviously the people running some of these "Approved" programs don't think so. Scary!! Thanks, Tim ----- Original Message ----- From: To: Sent: Wednesday, August 31, 2011 12:03 PM Subject: Histonet Digest, Vol 93, Issue 47 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Re:peggy wenk comments on HT/HTL practical - To stick a Pin (joelle weaver) ---------------------------------------------------------------------- Message: 1 Date: Wed, 31 Aug 2011 16:41:25 +0000 From: joelle weaver Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin To: , , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes, the old you need experience to get the job, and you need the job to get experience.... Joelle Weaver MAOM, BA, (HTL) ASCP > From: ree3@leicester.ac.uk > To: MLunetta@luhcares.org; histonet@lists.utsouthwestern.edu > Date: Wed, 31 Aug 2011 10:31:25 +0100 > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > CC: > > If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recently graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. > > Cheers > > Richard Edwards > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta > Sent: 30 August 2011 18:59 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > > Hey all, > > I found Peggy's comments on why the practical was discontinued to be > very interesting. Of late I have had some experience with a new HT that > graduated from a program and passed the current HT exam. > So, as they say in Great Britain, to stick a pin in the ASCP reasons. > > This new fresh and shiny HT has all the book knowledge we needed them to > have. What they did not have was any technical skills. > 1) never used a microscope or centrifuge. > 2) no special staining experience > 3) no embedding experience > 4) no cutting experience > > When they cut or embed they are no were near the speed, accuracy or > quality that is needed in our industry. While they can answer any > question you ask them they just do not have the technical skills one > would expect from a new graduate. > > I have learned several lessons from this experience. > > 1) I am so very glad I was one of the last HT's to have taken the > practical > 2) Any new HT's will be taking a practical if I am involved in the > selection process. > 3) I will question they quality of any new HT from this particular > program > > While I am sure that there are many new HT's that do have the skills > needed, this one experience has caused me to be more cautious. > > Respectfully, > > Matt Lunetta > BS, HT (ASCP) > > > > > Message: 2 > Date: Tue, 30 Aug 2011 18:09:46 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical > To: "'Bob Richmond'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Dr. Richmond > Here in Austria we have a job open for a pathologist with 5 years > experience. ;) > Please, think it over to come. Lovely mountains, lovely techs... > > It sounds, like you are from that sort of pathologist techs dream of. > Gudrun > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob > Richmond > Gesendet: Dienstag, 30. August 2011 04:43 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical > > I really appreciate Peggy Wenk's analysis of the practical examination > and why it had to be dropped. I never really understood the issue > before. > > I must confess I always enjoyed helping the prospective examinee > obtain exactly the right tissue. > > "No, this endometrium is poorly preserved. We'll arrange with surgery > for a completely fresh specimen - I'll block it initially for the > diagnosis, then we'll fix it overnight and then block it exactly to > specifications. - Ick - this one's been curetted - we'll get another > one". > > "I'll block the margins of this colon resection specimen, then we'll > pin a portion of tissue onto paraffin and fix it flat overnight." > > "Next time I do an autopsy we'll get a lumbar spinal cord in the > intact dura. I'll open the dura dorsally and ventrally with iridectomy > scissors, then we'll hang it in neutral buffered formalin for two > days. Then I'll tie the dura and dependent nerves with a cotton > string. When you embed you'll remove the string, taking care that dura > and nerves remain in position. After that it's all yours. If it > doesn't work the first time, we've got three more levels in the jar." > > OK, I'm a geek, I'm 72 years old, I got a right. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 93, Issue 47 **************************************** From joelleweaver <@t> hotmail.com Wed Aug 31 13:22:50 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 31 13:22:56 2011 Subject: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical In-Reply-To: <001801cc6808$d4aa0100$e001a8c0@cdd.loc> References: <20110831170305.23A3E13C37D5@barracuda.crvinc.net>, <001801cc6808$d4aa0100$e001a8c0@cdd.loc> Message-ID: Yes, that was what I was aluding to. I try to imagine that they just don't realize or have any knowledge of this, like me trying to fly a plane, since you mentioned pilots. It is better for me to consider it this way than just gettig upset or frustrated. From what I have seen in the education, the perception of some adminstrators is that histology is something that is quite simple and easily mastered, like "flipping burgers" . But the ones I know have never been in a histology lab, which sure didn't help.Maybe we need to educate some of the educators? In order to stay positive and forward looking, we can continue to try to develop though those that come in if we have the ability in staff, resources etc. Joelle Weaver MAOM, BA, (HTL) ASCP > From: thiggins@cddmedical.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 31 Aug 2011 13:07:21 -0500 > Subject: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical > > How would the expression, "you need experience to get the job, and you need > the job to get experience" work if a person went through flight school to be > a pilot but did not receive any actual flight training but was then allowed > to get a job as a pilot flying a commuter plane in charge of peoples lives. > Maybe actual training is not as big a deal if you are flipping burgers but > Histology is slightly more important than that. At least I feel it is, > obviously the people running some of these "Approved" programs don't think > so. > > Scary!! > > Thanks, > > Tim > ----- Original Message ----- > From: > To: > Sent: Wednesday, August 31, 2011 12:03 PM > Subject: Histonet Digest, Vol 93, Issue 47 > > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Re:peggy wenk comments on HT/HTL practical - To stick a > Pin (joelle weaver) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 31 Aug 2011 16:41:25 +0000 > From: joelle weaver > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - > To stick a Pin > To: , , Histonet > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Yes, the old you need experience to get the job, and you need the job to get > experience.... > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > > From: ree3@leicester.ac.uk > > To: MLunetta@luhcares.org; histonet@lists.utsouthwestern.edu > > Date: Wed, 31 Aug 2011 10:31:25 +0100 > > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To > stick a Pin > > CC: > > > > If you do not employ recently trained individuals, how on earth are they > going to obtain the experience that Matt craves, it's beyond me. My daughter > recently qualified as an Occupational Therapist, when she asked for the > reason that she was unsuccessful in obtaining a post, they said that as a > recently graduated student she had no experience, what rubbish!!.Thankfully > she has now obtained a position under more enlightened management that is to > be found here!. > > > > Cheers > > > > Richard Edwards > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew > Lunetta > > Sent: 30 August 2011 18:59 > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick > a Pin > > > > Hey all, > > > > I found Peggy's comments on why the practical was discontinued to be > > very interesting. Of late I have had some experience with a new HT that > > graduated from a program and passed the current HT exam. > > So, as they say in Great Britain, to stick a pin in the ASCP reasons. > > > > This new fresh and shiny HT has all the book knowledge we needed them to > > have. What they did not have was any technical skills. > > 1) never used a microscope or centrifuge. > > 2) no special staining experience > > 3) no embedding experience > > 4) no cutting experience > > > > When they cut or embed they are no were near the speed, accuracy or > > quality that is needed in our industry. While they can answer any > > question you ask them they just do not have the technical skills one > > would expect from a new graduate. > > > > I have learned several lessons from this experience. > > > > 1) I am so very glad I was one of the last HT's to have taken the > > practical > > 2) Any new HT's will be taking a practical if I am involved in the > > selection process. > > 3) I will question they quality of any new HT from this particular > > program > > > > While I am sure that there are many new HT's that do have the skills > > needed, this one experience has caused me to be more cautious. > > > > Respectfully, > > > > Matt Lunetta > > BS, HT (ASCP) > > > > > > > > > > Message: 2 > > Date: Tue, 30 Aug 2011 18:09:46 +0200 > > From: "Gudrun Lang" > > Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical > > To: "'Bob Richmond'" > > Cc: histonet@lists.utsouthwestern.edu > > Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> > > Content-Type: text/plain; charset="iso-8859-1" > > > > Dear Dr. Richmond > > Here in Austria we have a job open for a pathologist with 5 years > > experience. ;) > > Please, think it over to come. Lovely mountains, lovely techs... > > > > It sounds, like you are from that sort of pathologist techs dream of. > > Gudrun > > > > > > -----Urspr?ngliche Nachricht----- > > Von: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob > > Richmond > > Gesendet: Dienstag, 30. August 2011 04:43 > > An: histonet@lists.utsouthwestern.edu > > Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical > > > > I really appreciate Peggy Wenk's analysis of the practical examination > > and why it had to be dropped. I never really understood the issue > > before. > > > > I must confess I always enjoyed helping the prospective examinee > > obtain exactly the right tissue. > > > > "No, this endometrium is poorly preserved. We'll arrange with surgery > > for a completely fresh specimen - I'll block it initially for the > > diagnosis, then we'll fix it overnight and then block it exactly to > > specifications. - Ick - this one's been curetted - we'll get another > > one". > > > > "I'll block the margins of this colon resection specimen, then we'll > > pin a portion of tissue onto paraffin and fix it flat overnight." > > > > "Next time I do an autopsy we'll get a lumbar spinal cord in the > > intact dura. I'll open the dura dorsally and ventrally with iridectomy > > scissors, then we'll hang it in neutral buffered formalin for two > > days. Then I'll tie the dura and dependent nerves with a cotton > > string. When you embed you'll remove the string, taking care that dura > > and nerves remain in position. After that it's all yours. If it > > doesn't work the first time, we've got three more levels in the jar." > > > > OK, I'm a geek, I'm 72 years old, I got a right. > > > > Bob Richmond > > Samurai Pathologist > > Knoxville TN > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 93, Issue 47 > **************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SDattili <@t> stormontvail.org Wed Aug 31 12:43:30 2011 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Wed Aug 31 13:27:25 2011 Subject: [Histonet] RE: Histonet Digest, Vol 93, Issue 46 In-Reply-To: Message-ID: Hi all, Pam speaks to the real reason we have shortages: The lack of educational and degree-track programs at colleges and universities across the United States. As for me, I would love to hire a "shiny" new HT with no practical experience. We can give him or her that experience in our lab. We have done it many times before. What is difficult is to find people with a professional mind-set who have taken it upon themselves to get the necessary education and who see Histology as a career and not something you do while you are waiting to get into med-,vet- or nursing school. Encouraging professionalism is a priority. My hospital has what we call a "professional ladder." It was developed in conjunction with our campaign to become a Magnet hospital under the American Nurses Credentialing Center. A program for recognition of professional nurses was developed (called the Professional Nurse Contribution Ladder). It offers the ability to be recognized and rewarded at 3 different levels of accomplishment based on their clinical practice, continuing education, service to the community, etc. Our administration offered the same program to other professional classes in our healthcare system: laboratorian, pharmacists, respiratory therapists, etc. The laboratory launched our "Professional Laboratory Contribution Ladder" shortly after nursing went live with their program. Lab professionals holding certification can apply every two years for one of 3 levels offered, with each level being more difficult than the one before. I'm proud to say that histotechs that have their HT or HTL certification have been included in that program from the very beginning. Histotechs are also given funding for educational events on equal footing with all other professionals in our lab. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From TNMayer <@t> mdanderson.org Wed Aug 31 13:37:05 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Aug 31 13:36:49 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - In-Reply-To: References: Message-ID: Candace, I was much in the same boat as the other person you mentioned. I trained at small veterinary lab and sat for the exam. When I relocated and got work in a full service human hospital, it was culture shock. It took me a year or so to get to where I need to be. That is why I got into education, it not only ensures that newbies get what they need, but it makes sure that I really know my stuff. Hats off to my trainers (Cheryl, Del, Hal and Mae), the really to the time to ensure that I could do my job! The phrase that I hate the most is "where I trained...", I used it and now I know how annoying it can be. Thank goodness there are people who are willing and able to take the time to train newbies. Without them I wouldn't be where I am today. There are good things to be said for those of us who have OJT, and for those who completed a formal program. OJT people usually are more disciplined because they have to do it on there own. Those who have completed a program have the advantage of receiving theory for what they do. OJT people usually troubleshoot very well, and those in a program have the advantage of varied experience, before being hired. I did the practical and they took off for lint from the gauze on my slides, (go figure). Redid it and passed, without my chemistry background it would have been hard to pass the written though. Studying on my own took a lot of discipline. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ---------------------------------------------------------------------- Message: 1 Date: Wed, 31 Aug 2011 08:46:04 -0700 (PDT) From: Candice Smoots Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin To: Histonet Message-ID: <1314805564.16696.YahooMailNeo@web125403.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If my memory severs me correctly.....? is not "required" that a canidate must also have lab experience inorder to take the exam wheather it be from a histotech program or on the job training. So anyone who becomes HT/HTL certified should not only know basic theory but should also have atleast basic experience in micotomy, staining, fixation etc. I thought that this was the case for any of the routes that it takes to become certified. ? What I have found is that a person can start out in a specialty lab and work thier way up ?and get the work experience that is required to sit for the exam however because they only have experience in THAT lab, they are in a disadvantage because they do not have routine experience. ? For example, I know a person who started out in? a research lab and was trained and worked thier way up in about 3 years. They studied and sat for the exam because they had the required work experience and passed. They are now certified. However, whenn they got employed at a hospital that mostly did routines, they did have to be trained a little to undertsand rouine histology. They had never done basic trichromes?and they were?slow at sectioning because speed was not as important where they were from.?But the basic theory and micotmy, he had. ? I say all this to say...... Just because a person has the credientials does not mean that they have all the experience even though they are certified. So sometimes it may take a little training but i admit that it shouldnt take much as if they are a beginner... as they are certified. Thats just my opinion. I remain yours truely, Candice Camille From: Jennifer MacDonald To: Rene J Buesa Cc: "histonet@lists.utsouthwestern.edu" ; Matthew Lunetta ; JoyceWeems ; histonet-bounces@lists.utsouthwestern.edu; Shirley A. Powell Sent: Wednesday, August 31, 2011 10:21 AM Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Graduating students in histotech programs DO clinical rotations.? They have hands-on experience.? They are not just learning the theory from books. Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 08/31/2011 07:54 AM From joelleweaver <@t> hotmail.com Wed Aug 31 13:53:02 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 31 13:53:06 2011 Subject: [Histonet] RE: Histonet Digest, Vol 93, Issue 46 In-Reply-To: References: , Message-ID: That is great that your organization does such a career ladder and fosters professionalism. If more places adopted this, it might certainly be beneficial. Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Wed, 31 Aug 2011 12:43:30 -0500 > From: SDattili@stormontvail.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Histonet Digest, Vol 93, Issue 46 > > Hi all, > Pam speaks to the real reason we have shortages: The lack of educational and degree-track programs at colleges and universities across the United States. As for me, I would love to hire a "shiny" new HT with no practical experience. We can give him or her that experience in our lab. We have done it many times before. What is difficult is to find people with a professional mind-set who have taken it upon themselves to get the necessary education and who see Histology as a career and not something you do while you are waiting to get into med-,vet- or nursing school. > > Encouraging professionalism is a priority. My hospital has what we call a "professional ladder." It was developed in conjunction with our campaign to become a Magnet hospital under the American Nurses Credentialing Center. A program for recognition of professional nurses was developed (called the Professional Nurse Contribution Ladder). It offers the ability to be recognized and rewarded at 3 different levels of accomplishment based on their clinical practice, continuing education, service to the community, etc. Our administration offered the same program to other professional classes in our healthcare system: laboratorian, pharmacists, respiratory therapists, etc. The laboratory launched our "Professional Laboratory Contribution Ladder" shortly after nursing went live with their program. Lab professionals holding certification can apply every two years for one of 3 levels offered, with each level being more difficult than the one before. I'm proud to say that histotechs that have their HT or HTL certification have been included in that program from the very beginning. Histotechs are also given funding for educational events on equal footing with all other professionals in our lab. > > Regards, > Shelley D'Attilio MT(ASCP) > Manager, Chemistry, Cytology and Histology > Dept. of Pathology and Laboratory Medicine > Stormont-Vail HealthCare > Topeka, Kansas > > > > > > > > NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. > > ****************************************************************************************************************** > > The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. > > ****************************************************************************************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Aug 31 13:54:28 2011 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Aug 31 13:54:32 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: Message-ID: <986039693.683988.1314816868873.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I will clarify.? This person worked in the gross room as a PA and decided he wanted an HT.? So he watched over the shoulders of the histologists and learned enough to see the basics and then studied for the exam without ever cutting or staining a slide in Histology.? His theory was -?I cut frozens and do H&Es it won't be hard to pass a test with no practical and no one is checking to really see what I know besides what I learned in books and through acquiring testing examples so why not.? Guess what it was enough and he has an HT now.? I don't believe he has ever worked in the field as he is gone now and somewhere out of state. Pam ----- Original Message ----- From: "Emily Sours" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, August 31, 2011 12:52:43 PM Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick????????a Pin How do you become a certified HT and not have any lab experience?! That's crazy. Not that i know anything about being an HT, but I'm a lab tech and I can't imagine going into the job never having been in a lab at all. ?What exactly do they teach you?! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Aug 31 14:11:19 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Aug 31 14:11:30 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <986039693.683988.1314816868873.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <986039693.683988.1314816868873.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <8D7C2D242DBD45498006B21122072BF84940D483@MCINFRWEM003.ucsfmedicalcenter.org> But, to take the test you need an affidavit from the pathologist that you worked in the histology lab for at least a year. So something fishy there... Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, August 31, 2011 11:54 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I will clarify.? This person worked in the gross room as a PA and decided he wanted an HT.? So he watched over the shoulders of the histologists and learned enough to see the basics and then studied for the exam without ever cutting or staining a slide in Histology.? His theory was -?I cut frozens and do H&Es it won't be hard to pass a test with no practical and no one is checking to really see what I know besides what I learned in books and through acquiring testing examples so why not.? Guess what it was enough and he has an HT now.? I don't believe he has ever worked in the field as he is gone now and somewhere out of state. Pam ----- Original Message ----- From: "Emily Sours" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, August 31, 2011 12:52:43 PM Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick????????a Pin How do you become a certified HT and not have any lab experience?! That's crazy. Not that i know anything about being an HT, but I'm a lab tech and I can't imagine going into the job never having been in a lab at all. ?What exactly do they teach you?! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Aug 31 14:16:37 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Aug 31 14:17:06 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <8D7C2D242DBD45498006B21122072BF84940D483@MCINFRWEM003.ucsfmedicalcenter.org> References: <986039693.683988.1314816868873.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> <8D7C2D242DBD45498006B21122072BF84940D483@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32030D8F5F@Mail2Node2.ad.uams.edu> Tim, I understand that and he got one to sign it!! So it can be done and yes it is not legal or fair and unfortunately, it is not the first time I have heard of it just the first time I actually could verify. Way too late when I got here and it shows we have flaws in the system that are not being seen. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 31, 2011 2:11 PM To: 'Pam Marcum'; Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin But, to take the test you need an affidavit from the pathologist that you worked in the histology lab for at least a year. So something fishy there... Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, August 31, 2011 11:54 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I will clarify.? This person worked in the gross room as a PA and decided he wanted an HT.? So he watched over the shoulders of the histologists and learned enough to see the basics and then studied for the exam without ever cutting or staining a slide in Histology.? His theory was -?I cut frozens and do H&Es it won't be hard to pass a test with no practical and no one is checking to really see what I know besides what I learned in books and through acquiring testing examples so why not.? Guess what it was enough and he has an HT now.? I don't believe he has ever worked in the field as he is gone now and somewhere out of state. Pam ----- Original Message ----- From: "Emily Sours" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, August 31, 2011 12:52:43 PM Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick????????a Pin How do you become a certified HT and not have any lab experience?! That's crazy. Not that i know anything about being an HT, but I'm a lab tech and I can't imagine going into the job never having been in a lab at all. ?What exactly do they teach you?! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From billodonnell <@t> catholichealth.net Wed Aug 31 14:21:00 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Aug 31 14:21:09 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin In-Reply-To: <4E5E1B2D020000A8000650C3@ns.luhcares.org> References: <4E5E1B2D020000A8000650C3@ns.luhcares.org> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939519260F@chimsx08.CHI.catholichealth.net> Here's a man with a beer on his mind (just barley) Some typos ar just closer to my heart than others. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Wednesday, August 31, 2011 12:30 PM To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu; tpodawiltz@lrgh.org; b-frederick@northwestern.edu; hborgeri@wakehealth.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin Tom, I feel that this is very important conversation, not whining about the situation but shining a very bright light on an important subject. I agree that it is an opportunity to help shape a new HT if several factors are in place, the facility can afford the time, cost and personnel that it takes to train. Oh by and by the new HT is coming along and it will take months (much longer than the 3 probationary) to get them to the right place, luckily we can afford (just barley) the time, cost and personnel. At this time 2 months into the process they are not cutting small BX, STATS, embedding of BX, no immuno work and are not to be left alone in the lab. We are hopeful for a good outcome. Matt Lunetta BS HT (ASCP) >>> "Podawiltz, Thomas" 08/31/11 8:59 AM >>> I have had one tech finish an online HT course and currently a second person looking at going the online route to her HT. In both cases as their supervisor I have had to sign on as their in house trainer. As a trainer it is my job to make sure that they know how to function well in Histology when they are finished. My first tech was working elsewhere when she started school and receive little support with her studies, one of the main reasons she came to work for me was how appalled I was to hear that she was getting not support. By the time she was finished I would of let her work on any specimen removed from either myself or anyone in my family. We get the HT's that we work to get. As a supervisor and trainer, I am only as good as my staff makes me look. If I can go on vacation and the lab does not miss a beat, then I have done my job correctly. So to everyone that gets a fresh tech who got short changed on their training, don't whine about it, take it as an opportunity to shape them to the HT that you need them to be. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Wednesday, August 31, 2011 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin You might be surprised I too agree with Joyce and Richard. I understand very well that a new graduate will not be up to the skill level of an individual that has been working for a while. What I am surprised about is that this program seemed to teach to pass the test and has left all of the technical skills left to be taught by the persons 1st job. This could lead to several painful experiences for not only the facility but the new HT. Were is the disconnect. If a person is doing the OJT route they need to have at least one year of experience signed-off by a pathologist. If a person goes through a program who is responsible for making sure that the base-skills are there? Is there not some standards that a new graduate should be able to cut/embed so many blocks in an hour? Is it reasonable for a new graduate to take 1.5 hours to cut 5 (uterus, appendix, tonsil) one-cuts? From facing to lifting the slide off the water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut blocks? So Richard is also right how is a new grad to get experiance without that 1st gig? But how much resposiblity should be placed on the 1st gig to train a new HT that is supose to have base skills in lab equipment, cutting, embeding, staining etal? More thoughts.... ciao Matt Lunetta BS HT(ASCP) Edwards, Richard E." ree3@leicester.ac.uk If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recen tly graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. Cheers Richard Edwards >>> "Shirley A. Powell" 08/30/11 6:32 PM >>> I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is nee ded in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issu e before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Aug 31 14:35:02 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Aug 31 14:35:27 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32030D8F5F@Mail2Node2.ad.uams.edu> References: <986039693.683988.1314816868873.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> <8D7C2D242DBD45498006B21122072BF84940D483@MCINFRWEM003.ucsfmedicalcenter.org> <41D3A1AF6FEF0643BDC89E0516A6EA32030D8F5F@Mail2Node2.ad.uams.edu> Message-ID: <4E5E8CE6.2070509@pathology.washington.edu> Just curious, does NAACLS perform inspections like CAP to re-accredit and are all the programs out there NAACLS accredited? Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 8/31/2011 12:16 PM, Marcum, Pamela A wrote: > Tim, I understand that and he got one to sign it!! So it can be done and yes it is not legal or fair and unfortunately, it is not the first time I have heard of it just the first time I actually could verify. Way too late when I got here and it shows we have flaws in the system that are not being seen. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Wednesday, August 31, 2011 2:11 PM > To: 'Pam Marcum'; Emily Sours > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > > But, to take the test you need an affidavit from the pathologist that you worked in the histology lab for at least a year. So something fishy there... > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum > Sent: Wednesday, August 31, 2011 11:54 AM > To: Emily Sours > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > > > > I will clarify. This person worked in the gross room as a PA and decided he wanted an HT. So he watched over the shoulders of the histologists and learned enough to see the basics and then studied for the exam without ever cutting or staining a slide in Histology. His theory was - I cut frozens and do H&Es it won't be hard to pass a test with no practical and no one is checking to really see what I know besides what I learned in books and through acquiring testing examples so why not. Guess what it was enough and he has an HT now. I don't believe he has ever worked in the field as he is gone now and somewhere out of state. > > > > Pam > > > > > ----- Original Message ----- > > > From: "Emily Sours" > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, August 31, 2011 12:52:43 PM > Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > > How do you become a certified HT and not have any lab experience?! > That's crazy. > Not that i know anything about being an HT, but I'm a lab tech and I can't > imagine going into the job never having been in a lab at all. What exactly > do they teach you?! > > Emily > > > A great book should leave you with many experiences, and slightly exhausted. > You should live several lives while reading it. > -William Styron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, > use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message.. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Aug 31 14:50:35 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Wed Aug 31 14:50:40 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin Message-ID: They do. I think most are accredited. Seems to be some loop holes, or more detailed process might be needed? Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Victor Tobias Date: Wed, 31 Aug 2011 19:35:02 To: Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin ? Just curious, does NAACLS perform inspections like CAP to re-accredit and are all the programs out there NAACLS accredited? Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 8/31/2011 12:16 PM, Marcum, Pamela A wrote: > Tim, I understand that and he got one to sign it!!? So it can be done and yes it is not legal or fair and unfortunately, it is not the first time I have heard of it just the first time I actually could verify.? Way too late when I got here and it shows we have flaws in the system that are not being seen. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Wednesday, August 31, 2011 2:11 PM > To: 'Pam Marcum'; Emily Sours > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > > But, to take the test you need an affidavit from the pathologist that you worked in the histology lab for at least a year. So something fishy there... > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum > Sent: Wednesday, August 31, 2011 11:54 AM > To: Emily Sours > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin > > > > I will clarify.? This person worked in the gross room as a PA and decided he wanted an HT.? So he watched over the shoulders of the histologists and learned enough to see the basics and then studied for the exam without ever cutting or staining a slide in Histology.? His theory was - I cut frozens and do H&Es it won't be hard to pass a test with no practical and no one is checking to really see what I know besides what I learned in books and through acquiring testing examples so why not.? Guess what it was enough and he has an HT now.? I don't believe he has ever worked in the field as he is gone now and somewhere out of state. > > > > Pam > > > > > ----- Original Message ----- > > > From: "Emily Sours" > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, August 31, 2011 12:52:43 PM > Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick??????? a Pin > > How do you become a certified HT and not have any lab experience?! > That's crazy. > Not that i know anything about being an HT, but I'm a lab tech and I can't > imagine going into the job never having been in a lab at all.? What exactly > do they teach you?! > > Emily > > > A great book should leave you with many experiences, and slightly exhausted. > You should live several lives while reading it. > -William Styron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information.? Any unauthorized review, > use, disclosure or distribution is prohibited.? If you are not the > intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message.. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rceades <@t> gmail.com Wed Aug 31 14:52:06 2011 From: rceades <@t> gmail.com (Eric Eades) Date: Wed Aug 31 14:52:10 2011 Subject: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical In-Reply-To: References: <20110831170305.23A3E13C37D5@barracuda.crvinc.net> <001801cc6808$d4aa0100$e001a8c0@cdd.loc> Message-ID: It seems from these comments that not all schools are created equal. I just graduated from a one-year program in Histotechnology at Clover Park Technical College in Lakewood, WA and I can assure you that no one graduated unless they 1) Understood, and could preform by hand, every step in the histology workflow from grossing to coverslipping. 2) Completed a 300-hour clinical rotation with exposure to a variety of automated instruments and the production rate expected of a tech working in a clinical lab. 3) Demonstrated a respect for the profession and the passion to continue learning. I pity the HT candidate who enters the workforce with a less thorough preperation for what he's in for! -Eric Eades On Wed, Aug 31, 2011 at 11:22 AM, joelle weaver wrote: > > Yes, that was what I was aluding to. I try to imagine that they just don't > realize or have any knowledge of this, like me trying to fly a plane, since > you mentioned pilots. It is better for me to consider it this way than just > gettig upset or frustrated. From what I have seen in the education, the > perception of some adminstrators is that histology is something that is > quite simple and easily mastered, like "flipping burgers" . But the ones I > know have never been in a histology lab, which sure didn't help.Maybe we > need to educate some of the educators? In order to stay positive and forward > looking, we can continue to try to develop though those that come in if we > have the ability in staff, resources etc. > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > > From: thiggins@cddmedical.com > > To: histonet@lists.utsouthwestern.edu > > Date: Wed, 31 Aug 2011 13:07:21 -0500 > > Subject: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical > > > > How would the expression, "you need experience to get the job, and you > need > > the job to get experience" work if a person went through flight school to > be > > a pilot but did not receive any actual flight training but was then > allowed > > to get a job as a pilot flying a commuter plane in charge of peoples > lives. > > Maybe actual training is not as big a deal if you are flipping burgers > but > > Histology is slightly more important than that. At least I feel it is, > > obviously the people running some of these "Approved" programs don't > think > > so. > > > > Scary!! > > > > Thanks, > > > > Tim > > ----- Original Message ----- > > From: > > To: > > Sent: Wednesday, August 31, 2011 12:03 PM > > Subject: Histonet Digest, Vol 93, Issue 47 > > > > > > Send Histonet mailing list submissions to > > histonet@lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request@lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner@lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > > 1. RE: Re:peggy wenk comments on HT/HTL practical - To stick a > > Pin (joelle weaver) > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Wed, 31 Aug 2011 16:41:25 +0000 > > From: joelle weaver > > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - > > To stick a Pin > > To: , , Histonet > > > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > > > > Yes, the old you need experience to get the job, and you need the job to > get > > experience.... > > > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > > > > > From: ree3@leicester.ac.uk > > > To: MLunetta@luhcares.org; histonet@lists.utsouthwestern.edu > > > Date: Wed, 31 Aug 2011 10:31:25 +0100 > > > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To > > stick a Pin > > > CC: > > > > > > If you do not employ recently trained individuals, how on earth are > they > > going to obtain the experience that Matt craves, it's beyond me. My > daughter > > recently qualified as an Occupational Therapist, when she asked for the > > reason that she was unsuccessful in obtaining a post, they said that as a > > recently graduated student she had no experience, what > rubbish!!.Thankfully > > she has now obtained a position under more enlightened management that is > to > > be found here!. > > > > > > Cheers > > > > > > Richard Edwards > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew > > Lunetta > > > Sent: 30 August 2011 18:59 > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To > stick > > a Pin > > > > > > Hey all, > > > > > > I found Peggy's comments on why the practical was discontinued to be > > > very interesting. Of late I have had some experience with a new HT that > > > graduated from a program and passed the current HT exam. > > > So, as they say in Great Britain, to stick a pin in the ASCP reasons. > > > > > > This new fresh and shiny HT has all the book knowledge we needed them > to > > > have. What they did not have was any technical skills. > > > 1) never used a microscope or centrifuge. > > > 2) no special staining experience > > > 3) no embedding experience > > > 4) no cutting experience > > > > > > When they cut or embed they are no were near the speed, accuracy or > > > quality that is needed in our industry. While they can answer any > > > question you ask them they just do not have the technical skills one > > > would expect from a new graduate. > > > > > > I have learned several lessons from this experience. > > > > > > 1) I am so very glad I was one of the last HT's to have taken the > > > practical > > > 2) Any new HT's will be taking a practical if I am involved in the > > > selection process. > > > 3) I will question they quality of any new HT from this particular > > > program > > > > > > While I am sure that there are many new HT's that do have the skills > > > needed, this one experience has caused me to be more cautious. > > > > > > Respectfully, > > > > > > Matt Lunetta > > > BS, HT (ASCP) > > > > > > > > > > > > > > > Message: 2 > > > Date: Tue, 30 Aug 2011 18:09:46 +0200 > > > From: "Gudrun Lang" > > > Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical > > > To: "'Bob Richmond'" > > > Cc: histonet@lists.utsouthwestern.edu > > > Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> > > > Content-Type: text/plain; charset="iso-8859-1" > > > > > > Dear Dr. Richmond > > > Here in Austria we have a job open for a pathologist with 5 years > > > experience. ;) > > > Please, think it over to come. Lovely mountains, lovely techs... > > > > > > It sounds, like you are from that sort of pathologist techs dream of. > > > Gudrun > > > > > > > > > -----Urspr?ngliche Nachricht----- > > > Von: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob > > > Richmond > > > Gesendet: Dienstag, 30. August 2011 04:43 > > > An: histonet@lists.utsouthwestern.edu > > > Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical > > > > > > I really appreciate Peggy Wenk's analysis of the practical examination > > > and why it had to be dropped. I never really understood the issue > > > before. > > > > > > I must confess I always enjoyed helping the prospective examinee > > > obtain exactly the right tissue. > > > > > > "No, this endometrium is poorly preserved. We'll arrange with surgery > > > for a completely fresh specimen - I'll block it initially for the > > > diagnosis, then we'll fix it overnight and then block it exactly to > > > specifications. - Ick - this one's been curetted - we'll get another > > > one". > > > > > > "I'll block the margins of this colon resection specimen, then we'll > > > pin a portion of tissue onto paraffin and fix it flat overnight." > > > > > > "Next time I do an autopsy we'll get a lumbar spinal cord in the > > > intact dura. I'll open the dura dorsally and ventrally with iridectomy > > > scissors, then we'll hang it in neutral buffered formalin for two > > > days. Then I'll tie the dura and dependent nerves with a cotton > > > string. When you embed you'll remove the string, taking care that dura > > > and nerves remain in position. After that it's all yours. If it > > > doesn't work the first time, we've got three more levels in the jar." > > > > > > OK, I'm a geek, I'm 72 years old, I got a right. > > > > > > Bob Richmond > > > Samurai Pathologist > > > Knoxville TN > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > End of Histonet Digest, Vol 93, Issue 47 > > **************************************** > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkrupp <@t> deltacollege.edu Wed Aug 31 14:53:49 2011 From: jkrupp <@t> deltacollege.edu (Jon Krupp) Date: Wed Aug 31 14:54:01 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical In-Reply-To: <840447922.675966.1314806514331.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <840447922.675966.1314806514331.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: On Aug 31, 2011, at 9:01 AM, Pam Marcum wrote: > > > I have followed this for a while and was wondering why no one is bringing up the lack of Histology Schools and the small number of graduates they can produce per year as an issue. Online is fine if you have a lab to work in. However; one of the people no longer at this facility simply did his book work and an HT then offered himself as just that. He never cut a section or did a stain yet he passed so it is possible. > > > > Many of us are training OJTs again due to the lack of available people and the in our case the starting salary is low. It is said it is because we are not required to complete a BS for an HT only the HTL . It means we are doing as much as we can to train someone in only one lab with the small amount of time an understaffed Histology Laboratory has yet still have the best training for the field. We are not recognized fully as Laboratory Professionals yet so it is not really getting any better than it was years ago. Now we require a 2 year degree for education in science and not much help from the organizations that rule us beyond pay more money. Many of us are not able to go to meetings ( especially NSH ) due to costs to us personally as the hospitals and Universities are not paying for travel and very little for educational help. I may be coming late to this discussion, but I thought I would add my ideas. First, like many of you, I am amazed that an HT can be 'certified' without demonstrating practical ability as part of the process. Maybe if there is some OTJ training and experience that is verified it could work, but I am hearing that that is not a requirement. So, why are the educational opportunities for HT types limited? I can only share my experience here at Delta, a community college in California's central valley. We have lots of students desperate for a job. Many of them might be willing to give HT a try, but its not in the cards for now. I am part of an EM training program, we turn out skilled and competent electron microscopists. Many of the skills the EM students learn could easily be applied to HT. We have all the equipment for fixing, infiltrating, sectioning and staining. All I would need to turn out a few HT each year is the overhead to get the students signed off for their certificate. So why don't I do this? There are several reasons. Most important these days, we, the college, have no money to fund new programs. I have been told I must have a minimum of 20 students in a class to offer it. Getting 20 students into HT classes would be hard here, especially at this time and given the limited opportunities locally for any clinical or OTJ training to round out the curriculum. America Mastertech is located close by, and they have generously offered to help out, but it is impossible to get a program off the ground in the current fiscal situation in California. I would be happy to make some contribution to the training of HT's, but it's not going to happen until a number of things change. Jon Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu From joelleweaver <@t> hotmail.com Wed Aug 31 14:55:38 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Wed Aug 31 14:55:43 2011 Subject: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical Message-ID: Me too! I am glad you felt well prepared with your program, and they set clear standards. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Eric Eades Date: Wed, 31 Aug 2011 19:52:06 To: Cc: ; Subject: Re: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical It seems from these comments that not all schools are created equal.? I just graduated from a one-year program in Histotechnology at Clover Park Technical College in Lakewood, WA and I can assure you that no one graduated unless they 1) Understood, and could preform by hand, every step in the histology workflow from grossing to coverslipping.? 2)? Completed a 300-hour clinical rotation with exposure?to a variety of automated instruments and the production rate expected of a tech working in a clinical lab.? 3) Demonstrated a?respect for the profession and the passion to continue learning.? ? I pity the HT candidate who enters the workforce with a less thorough preperation for what he's in for! ? -Eric Eades ? ? On Wed, Aug 31, 2011 at 11:22 AM, joelle weaver > wrote: Yes, that was what I was aluding to. I try to imagine that they just don't realize or have any knowledge of this, like me trying to fly a plane, since you mentioned pilots. It is better for me to consider it this way than just gettig upset or frustrated. ?From what I have seen in the education, the perception of some adminstrators is that histology is something that is quite simple and easily mastered, like "flipping burgers" . But the ones I know have never been in a histology lab, which sure didn't help.Maybe we need to educate some of the educators? In order to stay positive and forward looking, we can continue to try to develop though those that come in if we have the ability in staff, resources etc. Joelle Weaver MAOM, BA, (HTL) ASCP > From: thiggins@cddmedical.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 31 Aug 2011 13:07:21 -0500 > Subject: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical > > How would the expression, "you need experience to get the job, and you need > the job to get experience" work if a person went through flight school to be > a pilot but did not receive any actual flight training but was then allowed > to get a job as a pilot flying a commuter plane in charge of peoples lives. > Maybe actual training is not as big a deal if you are flipping burgers but > Histology is slightly more important than that. At least I feel it is, > obviously the people running some of these "Approved" programs don't think > so. > > Scary!! > > Thanks, > > Tim > ----- Original Message ----- > From: > > To: > > Sent: Wednesday, August 31, 2011 12:03 PM > Subject: Histonet Digest, Vol 93, Issue 47 > > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Re:peggy wenk comments on HT/HTL practical - To stick a > Pin (joelle weaver) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 31 Aug 2011 16:41:25 +0000 > From: joelle weaver > > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - > To stick a Pin > To: >, >, Histonet > > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Yes, the old you need experience to get the job, and you need the job to get > experience.... > > Joelle Weaver MAOM, BA, (HTL) ASCP > > > > From: ree3@leicester.ac.uk > > To: MLunetta@luhcares.org ; histonet@lists.utsouthwestern.edu > > Date: Wed, 31 Aug 2011 10:31:25 +0100 > > Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To > stick a Pin > > CC: > > > > If you do not employ recently trained individuals, how on earth are they > going to obtain the experience that Matt craves, it's beyond me. My daughter > recently qualified as an Occupational Therapist, when she asked for the > reason that she was unsuccessful in obtaining a post, they said that as a > recently graduated student she had no experience, what rubbish!!.Thankfully > she has now obtained a position under more enlightened management that is to > be found here!. > > > > Cheers > > > > Richard Edwards > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of Matthew > Lunetta > > Sent: 30 August 2011 18:59 > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick > a Pin > > > > Hey all, > > > > I found Peggy's comments on why the practical was discontinued to be > > very interesting. Of late I have had some experience with a new HT that > > graduated from a program and passed the current HT exam. > > So, as they say in Great Britain, to stick a pin in the ASCP reasons. > > > > This new fresh and shiny HT has all the book knowledge we needed them to > > have. What they did not have was any technical skills. > > 1) never used a microscope or centrifuge. > > 2) no special staining experience > > 3) no embedding experience > > 4) no cutting experience > > > > When they cut or embed they are no were near the speed, accuracy or > > quality that is needed in our industry. While they can answer any > > question you ask them they just do not have the technical skills one > > would expect from a new graduate. > > > > I have learned several lessons from this experience. > > > > 1) I am so very glad I was one of the last HT's to have taken the > > practical > > 2) Any new HT's will be taking a practical if I am involved in the > > selection process. > > 3) I will question they quality of any new HT from this particular > > program > > > > While I am sure that there are many new HT's that do have the skills > > needed, this one experience has caused me to be more cautious. > > > > Respectfully, > > > > Matt Lunetta > > BS, HT (ASCP) > > > > > > > > > > Message: 2 > > Date: Tue, 30 Aug 2011 18:09:46 +0200 > > From: "Gudrun Lang" > > Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical > > To: "'Bob Richmond'" > > Cc: histonet@lists.utsouthwestern.edu > > Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at > > > Content-Type: text/plain; charset="iso-8859-1" > > > > Dear Dr. Richmond > > Here in Austria we have a job open for a pathologist with 5 years > > experience. ;) > > Please, think it over to come. Lovely mountains, lovely techs... > > > > It sounds, like you are from that sort of pathologist techs dream of. > > Gudrun > > > > > > -----Urspr?ngliche Nachricht----- > > Von: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu ] Im Auftrag von Bob > > Richmond > > Gesendet: Dienstag, 30. August 2011 04:43 > > An: histonet@lists.utsouthwestern.edu > > Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical > > > > I really appreciate Peggy Wenk's analysis of the practical examination > > and why it had to be dropped. I never really understood the issue > > before. > > > > I must confess I always enjoyed helping the prospective examinee > > obtain exactly the right tissue. > > > > "No, this endometrium is poorly preserved. We'll arrange with surgery > > for a completely fresh specimen - I'll block it initially for the > > diagnosis, then we'll fix it overnight and then block it exactly to > > specifications. - Ick - this one's been curetted - we'll get another > > one". > > > > "I'll block the margins of this colon resection specimen, then we'll > > pin a portion of tissue onto paraffin and fix it flat overnight." > > > > "Next time I do an autopsy we'll get a lumbar spinal cord in the > > intact dura. I'll open the dura dorsally and ventrally with iridectomy > > scissors, then we'll hang it in neutral buffered formalin for two > > days. Then I'll tie the dura and dependent nerves with a cotton > > string. When you embed you'll remove the string, taking care that dura > > and nerves remain in position. After that it's all yours. If it > > doesn't work the first time, we've got three more levels in the jar." > > > > OK, I'm a geek, I'm 72 years old, I got a right. > > > > Bob Richmond > > Samurai Pathologist > > Knoxville TN > > > >_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 93, Issue 47 > **************************************** > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Aug 31 15:41:07 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Aug 31 15:41:09 2011 Subject: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical In-Reply-To: References: <20110831170305.23A3E13C37D5@barracuda.crvinc.net> <001801cc6808$d4aa0100$e001a8c0@cdd.loc> Message-ID: I knew I couldn't get a job in a lab without experience, so I offered myself to anyone, anyone at all who was willing to teach me for free. Luckily I got an awesome job for a semester (volunteering) but it taught me a lot. You have to go out there and find yourself experience, you can't wait for it to fall in your lap. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Aug 31, 2011 at 3:52 PM, Eric Eades wrote: > It seems from these comments that not all schools are created equal. I > just > graduated from a one-year program in Histotechnology at Clover Park > Technical College in Lakewood, WA and I can assure you that no one > graduated > unless they 1) Understood, and could preform by hand, every step in the > histology workflow from grossing to coverslipping. 2) Completed a > 300-hour > clinical rotation with exposure to a variety of automated instruments and > the production rate expected of a tech working in a clinical lab. 3) > Demonstrated a respect for the profession and the passion to continue > learning. > > I pity the HT candidate who enters the workforce with a less thorough > preperation for what he's in for! > > -Eric Eades > > > > > From tpodawiltz <@t> lrgh.org Wed Aug 31 16:11:03 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Aug 31 16:15:57 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin In-Reply-To: <4E5E1B2D020000A8000650C3@ns.luhcares.org> References: <4E5E1B2D020000A8000650C3@ns.luhcares.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE66@LRGHEXVS1.practice.lrgh.org> Just to let you know, I don't let new techs near prostate, liver or any other kind of needle biopsy for at least a year. Why put them under that kind of pressure? I want them to stay in the field. I figure working for me is torture enough. We give new techs up to three years to get up to speed and pass the exam. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: Matthew Lunetta [MLunetta@luhcares.org] Sent: Wednesday, August 31, 2011 1:29 PM To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu; Podawiltz, Thomas; b-frederick@northwestern.edu; hborgeri@wakehealth.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Tom, I feel that this is very important conversation, not whining about the situation but shining a very bright light on an important subject. I agree that it is an opportunity to help shape a new HT if several factors are in place, the facility can afford the time, cost and personnel that it takes to train. Oh by and by the new HT is coming along and it will take months (much longer than the 3 probationary) to get them to the right place, luckily we can afford (just barley) the time, cost and personnel. At this time 2 months into the process they are not cutting small BX, STATS, embedding of BX, no immuno work and are not to be left alone in the lab. We are hopeful for a good outcome. Matt Lunetta BS HT (ASCP) >>> "Podawiltz, Thomas" 08/31/11 8:59 AM >>> I have had one tech finish an online HT course and currently a second person looking at going the online route to her HT. In both cases as their supervisor I have had to sign on as their in house trainer. As a trainer it is my job to make sure that they know how to function well in Histology when they are finished. My first tech was working elsewhere when she started school and receive little support with her studies, one of the main reasons she came to work for me was how appalled I was to hear that she was getting not support. By the time she was finished I would of let her work on any specimen removed from either myself or anyone in my family. We get the HT's that we work to get. As a supervisor and trainer, I am only as good as my staff makes me look. If I can go on vacation and the lab does not miss a beat, then I have done my job correctly. So to everyone that gets a fresh tech who got short changed on their training, don't whine about it, take it as an opportunity to shape them to the HT that you need them to be. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Wednesday, August 31, 2011 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin You might be surprised I too agree with Joyce and Richard. I understand very well that a new graduate will not be up to the skill level of an individual that has been working for a while. What I am surprised about is that this program seemed to teach to pass the test and has left all of the technical skills left to be taught by the persons 1st job. This could lead to several painful experiences for not only the facility but the new HT. Were is the disconnect. If a person is doing the OJT route they need to have at least one year of experience signed-off by a pathologist. If a person goes through a program who is responsible for making sure that the base-skills are there? Is there not some standards that a new graduate should be able to cut/embed so many blocks in an hour? Is it reasonable for a new graduate to take 1.5 hours to cut 5 (uterus, appendix, tonsil) one-cuts? From facing to lifting the slide off the water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut blocks? So Richard is also right how is a new grad to get experiance without that 1st gig? But how much resposiblity should be placed on the 1st gig to train a new HT that is supose to have base skills in lab equipment, cutting, embeding, staining etal? More thoughts.... ciao Matt Lunetta BS HT(ASCP) Edwards, Richard E." ree3@leicester.ac.uk If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recently graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. Cheers Richard Edwards >>> "Shirley A. Powell" 08/30/11 6:32 PM >>> I second that Joyce. sp ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their "Clinicals". They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is nee ded in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: "'Bob Richmond'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8B7976B131854ABC8DB236FAB5026851@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. "No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one". "I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight." "Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar." OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From COPPINM <@t> aruplab.com Wed Aug 31 16:53:20 2011 From: COPPINM <@t> aruplab.com (Coppin, Margaret) Date: Wed Aug 31 16:53:28 2011 Subject: [Histonet] CAP slides Message-ID: Hello, A few months back, there was a discussion on the list about CAP Proficiency Test slides that weren't staining as expected. Some of you had even contacted CAP and they acknowledged that there had been an issue. Any of you whose PT was affected, do you mind jogging my memory as to which PT's were the problem batch? Thanks in advance as always. Margaret ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From tkngflght <@t> yahoo.com Wed Aug 31 19:15:16 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Aug 31 19:15:20 2011 Subject: [Histonet] trolling for travelers! Message-ID: <1314836116.77090.YahooMailNeo@web39415.mail.mud.yahoo.com> Hi Everyone- ? Already traveling & want to stay busy? Looking to travel?? We need to add a few and?'season' a few new?techs for our travel team.? ? The conversation is fun--truly--and I'll answer all your questions to help you make the right decision for you and your family. ? 800.756.3309? ? (Also seeking grossing techs for five different permanent jobs around the country--get out of the cold/away from the hurricane--or both!) ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From lpwenk <@t> sbcglobal.net Wed Aug 31 20:02:37 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Aug 31 20:02:32 2011 Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin In-Reply-To: <8D7C2D242DBD45498006B21122072BF84940D483@MCINFRWEM003.ucsfmedicalcenter.org> References: <986039693.683988.1314816868873.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> <8D7C2D242DBD45498006B21122072BF84940D483@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <77D82F1F34C0428AB379A7754ACC42A3@HP2010> "Acceptable experience", for the on-the-job route (OJT), as defined by ASCP to sit for the HT or the HTL exam is the following: ". . . you must have experience, within the last ten years, in the following areas: ?Fixation ?Microtomy ?Processing ?Staining " So, the PA met the requirement: - they fix tissue in formalin and maybe something else - using a cryostat is microtomy - if they ever load the tissue on the processor, then they process (And if you think about it, freezing tissue is a type of processing - it's changing the consistency of the water in the cells) - H&E is a stain The same can be said for someone working in Mohs, who only does FS and H&E on skin. The same can apply to someone working in EM, who fixes with glut and osmium, uses an ultramicrotomy on resin blocks, stain with metal salts. They meet the criteria. As would someone working just in a GI lab, or with just rat tissue, or only doing IHC. There is no ASCP HT/HTL criteria of what type of tissues, what type of processing, which stains are required, how many/how fast. That allows for histotechs in many different labs working with very different tissues, stains, embedding media, microtomes, etc., to be eligible to sit for the histo exams. We don't want to make demands that are too strict, which would make a lot of people ineligible to take the exam. By making the requirements generic enough so that a lot of histotechs in a lot of different types of lab will be qualified to sit for the exam, unfortunately, there will be some people who slip in who may not really be qualified (like the PA). But remember, he had to have studied the book enough to pass the written exam. That says something. So again, it comes back to be supervisor who is thinking about hiring the person. Either have the person prove during the interview that they really can microtome paraffin blocks, do an H&E, and/or coverslip. OR, use the 3 months probation and get rid of the person if they can't perform the duties of a histotech. I think most histotech supervisors are nice people, but sometimes we are too nice. We don't want to hurt anyone's feelings. We like to give people lots of chances. But sometimes, we HAVE to be the supervisor, and do what's best of the lab and the patient. Peggy Wenk -----Original Message----- From: Morken, Timothy Sent: Wednesday, August 31, 2011 3:11 PM To: 'Pam Marcum' ; Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin But, to take the test you need an affidavit from the pathologist that you worked in the histology lab for at least a year. So something fishy there... Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, August 31, 2011 11:54 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I will clarify. This person worked in the gross room as a PA and decided he wanted an HT. So he watched over the shoulders of the histologists and learned enough to see the basics and then studied for the exam without ever cutting or staining a slide in Histology. His theory was - I cut frozens and do H&Es it won't be hard to pass a test with no practical and no one is checking to really see what I know besides what I learned in books and through acquiring testing examples so why not. Guess what it was enough and he has an HT now. I don't believe he has ever worked in the field as he is gone now and somewhere out of state. Pam ----- Original Message ----- From: "Emily Sours" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, August 31, 2011 12:52:43 PM Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin How do you become a certified HT and not have any lab experience?! That's crazy. Not that i know anything about being an HT, but I'm a lab tech and I can't imagine going into the job never having been in a lab at all. What exactly do they teach you?! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ernestinemiddleton <@t> yahoo.ca Wed Aug 31 20:23:54 2011 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Wed Aug 31 20:23:57 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Message-ID: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper.? Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org