[Histonet] Histo gel users?
morgancd <@t> charter.net
morgancd <@t> charter.net
Sat Apr 16 17:30:23 CDT 2011
The specimen does not need to be fixed it will fix in the NBF during
processing.
Dawn F. Morgan
> What about using it on specimens that are going to be tested for
> prognostic markers? Like Her 2 neu? Thanks Deb.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> histonet-request <@t> lists.utsouthwestern.edu
> Sent: Wednesday, April 13, 2011 9:10 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 89, Issue 13
>
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> Today's Topics:
>
> 1. Immunofluorescence and FISH Combined stating (Diane Kolins)
> 2. Von kossa stain (Webb, Dorothy L)
> 3. RE: Von kossa stain (Breeden, Sara)
> 4. Re: Histonet Digest, Vol 89, Issue 12 (Mark Elliott)
> 5. AW: [Histonet] Von kossa stain (Gudrun Lang)
> 6. Re: AW: [Histonet] Von kossa stain
> (Grantham, Andrea L - (algranth))
> 7. Staining using a Plastic Sytem (Mahesh Polavarapu)
> 8. Biopsy program for Sakura VIP 5/6 Processor (Evans, Andria B)
> 9. Re: Milestone MW (Rene J Buesa)
> 10. Re: Von kossa stain (Rene J Buesa)
> 11. RE: Von kossa stain (Jack Ratliff)
> 12. RE: Counterstain for dual chromogenic (Tony Henwood)
> 13. RE: Von kossa stain (Tony Henwood)
> 14. RE: Biopsy program for Sakura VIP 5/6 Processor (Tony Henwood)
> 15. RE: RE: Biopsy program for Sakura VIP 5/6 Processor
> (WILLIAM DESALVO)
> 16. RE: RE: Biopsy program for Sakura VIP 5/6 Processor (Tony
> Henwood)
> 17. Update regarding question on Plastic Embedding (Mahesh
> Polavarapu)
> 18. SP3 HER2 mAb (Richard Cartun)
> 19. FW: 1 H&E slide vs. 2 (Eugenia Thomas)
> 20. Ventana ultra (Barbara.Crill <@t> LPNT.net)
> 21. RE: FW: 1 H&E slide vs. 2 (Monfils, Paul)
> 22. RE: Ventana ultra (Sebree Linda A)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 12 Apr 2011 13:23:03 -0400
> From: Diane Kolins <Diane <@t> bioview.co.il>
> Subject: [Histonet] Immunofluorescence and FISH Combined stating
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>
> <!&!AAAAAAAAAAAYAAAAAAAAAKxbzBNp1TJAsnIO9XHEfgnCgAAAEAAAAKFMd/+8AfZLjHzeYTbktzQBAAAAAA==@bioview.co.il>
>
> Content-Type: text/plain; charset=us-ascii
>
> Where can I find a procedure for dual staining of blood and bone
> marrow
> smears using Vector anti-kappa and anti-lambda tagged with Coumarine
> followed by FISH hybridization. I am able to see what I think are
> plasma
> cells by their fluorescent blue cytoplasm and on the same slide
> (changing
> filters) I can see the red and green signal patterns.
>
>
> From: : diane <@t> bioview.co.il
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 12 Apr 2011 12:35:18 -0500
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Subject: [Histonet] Von kossa stain
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1 <@t> HPEMX3.HealthPartners.int>
>
> Content-Type: text/plain; charset="us-ascii"
>
> What is everyone using for their "light" when developing the silver in
> the VonKossa stain when you have no sunlight to use? We used to use a
> 60 watt lamp, but haven't done one for years and am bringing this
> stain back to our repetiore due to pathologist request. Thanks much!
>
> Dorothy Webb, HT (ASCP)
> Regions Histology Technical Supervisor
> 651-254-2962
>
>
>
>
> ________________________________
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to whom they
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> any use, dissemination, forwarding, printing, or copying of this
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>
> If you have received this e-mail in error, please immediately notify
> the HealthPartners Support Center by telephone at (952) 967-6600. You
> will be reimbursed for reasonable costs incurred in notifying us.
> HealthPartners R001.0
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 12 Apr 2011 11:52:49 -0600
> From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
> Subject: RE: [Histonet] Von kossa stain
> To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <4D14F0FC9316DD41972D5F03C070908B02E477BD <@t> nmdamailsvr.nmda.ad.nmsu.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Funny you should ask... just last week I had two requests for a Von
> Kossa. That's when I found out that, despite the fact that we just
> moved into a brand new building with all the bells and whistles, there
> was not one single UV light in any of the many hoods that had been
> installed. Heaven forbid I could even find an incandescent bulb (we
> are a "green building"). So, I prepped my slides, put them in a clear
> glass Coplin jar and parked the jar on the hood of my car for an hour.
>
> Works like a charm. This all depends, naturally, on (1) if you even
> HAVE sunshine where you live; (2) how far a walk it is to the
> sunshine,
> and (3) whether you have a car hood on which to park the Coplin jar.
> Needless to say, out of the line of sight of curious onlookers with
> sticky fingers and no business wondering what that glass jar is...
> Nonetheless, it works just fine! May the Force be with you.
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 12 Apr 2011 11:16:16 -0700
> From: "Mark Elliott" <Mark.Elliott <@t> hli.ubc.ca>
> Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 12
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4DA43480020000D60006111F <@t> mail.mrl.ubc.ca>
> Content-Type: text/plain; charset=US-ASCII
>
>
> Date: Tue, 12 Apr 2011 11:37:17 -0400
> From: Eva Permaul <eca9 <@t> georgetown.edu>
> Subject: [Histonet] Counterstain for dual chromogenic
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4DA471AD.3040002 <@t> georgetown.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Eva
> I have been doing some double labelling using Vulcan Fast Red from
> Biocare and Vector Blue Alkaline Phosphatase Substrate Kit III from
> Vector Labs. For counter stain I use Vector Methyl Green and the
> combination works great and looks quite nice.
> Mark
> Good morning,
> I have been attempting to do some dual chromogenic staining. I am
> using a mouse and a rabbit antibody. For the mouse antibody I am using
> an HRP-mouse secondary followed by AEC for visualization. For the
> rabbit antibody I am using a biotinylated rabbit secondary, followed
> by ABC-AP and vector blue. My problem is what to use as a
> counterstain. From everything I have read so far there isn't one that
> would work without having some problems. I was thinking of trying a
> very light Hematoxylin and see if it doesn't disrupt the vector blue
> visualization too much. Does anyone else have any suggestions?
> Thanks,
> Eva
>
> ***CONFIDENTIALITY NOTICE***
> This electronic message and any attachments are intended only for the
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>
> ------------------------------
>
> Message: 5
> Date: Tue, 12 Apr 2011 20:22:22 +0200
> From: "Gudrun Lang" <gu.lang <@t> gmx.at>
> Subject: AW: [Histonet] Von kossa stain
> To: "'Webb, Dorothy L'" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <13B705C625E74F1BB9337593D57C3658 <@t> dielangs.at>
> Content-Type: text/plain; charset="iso-8859-1"
>
> WE use just a simple desk-lamp and put the coplin jar directly under
> the
> bulb. And the desk-lamp is placed in the window.
>
> Bye Gudrun
>
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
> Webb,
> Dorothy L
> Gesendet: Dienstag, 12. April 2011 19:35
> An: 'histonet <@t> lists.utsouthwestern.edu'
> Betreff: [Histonet] Von kossa stain
>
> What is everyone using for their "light" when developing the silver in
> the
> VonKossa stain when you have no sunlight to use? We used to use a 60
> watt
> lamp, but haven't done one for years and am bringing this stain back
> to our
> repetiore due to pathologist request. Thanks much!
>
> Dorothy Webb, HT (ASCP)
> Regions Histology Technical Supervisor
> 651-254-2962
>
>
>
>
> ________________________________
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to whom they
> are
> addressed. If you are not the intended recipient or the individual
> responsible for delivering the e-mail to the intended recipient,
> please be
> advised that you have received this e-mail in error and that any use,
> dissemination, forwarding, printing, or copying of this e-mail is
> strictly
> prohibited.
>
> If you have received this e-mail in error, please immediately notify
> the
> HealthPartners Support Center by telephone at (952) 967-6600. You will
> be
> reimbursed for reasonable costs incurred in notifying us.
> HealthPartners
> R001.0
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 12 Apr 2011 11:35:47 -0700
> From: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
> Subject: Re: AW: [Histonet] Von kossa stain
> Cc: HISTONET <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <59222C2B-42C1-4EB6-8233-ED0FB7FCA354 <@t> email.arizona.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> We have intense sun here but my windows face the wrong way! At least I
> have windows. I just use a light bulb from the lamp we have over the
> coverslipping station. Works great.
>
> Andi
>
>
>
> On Apr 12, 2011, at 11:22 AM, Gudrun Lang wrote:
>
>> WE use just a simple desk-lamp and put the coplin jar directly under
>> the
>> bulb. And the desk-lamp is placed in the window.
>>
>> Bye Gudrun
>>
>> -----Ursprüngliche Nachricht-----
>> Von: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
>> Webb,
>> Dorothy L
>> Gesendet: Dienstag, 12. April 2011 19:35
>> An: 'histonet <@t> lists.utsouthwestern.edu'
>> Betreff: [Histonet] Von kossa stain
>>
>> What is everyone using for their "light" when developing the silver
>> in the
>> VonKossa stain when you have no sunlight to use? We used to use a 60
>> watt
>> lamp, but haven't done one for years and am bringing this stain back
>> to our
>> repetiore due to pathologist request. Thanks much!
>>
>> Dorothy Webb, HT (ASCP)
>> Regions Histology Technical Supervisor
>> 651-254-2962
>>
>>
>>
>>
>> ________________________________
>> This e-mail and any files transmitted with it are confidential and
>> are
>> intended solely for the use of the individual or entity to whom they
>> are
>> addressed. If you are not the intended recipient or the individual
>> responsible for delivering the e-mail to the intended recipient,
>> please be
>> advised that you have received this e-mail in error and that any use,
>> dissemination, forwarding, printing, or copying of this e-mail is
>> strictly
>> prohibited.
>>
>> If you have received this e-mail in error, please immediately notify
>> the
>> HealthPartners Support Center by telephone at (952) 967-6600. You
>> will be
>> reimbursed for reasonable costs incurred in notifying us.
>> HealthPartners
>> R001.0
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 12 Apr 2011 13:56:53 -0500
> From: Mahesh Polavarapu <polavarapu.mahesh <@t> gmail.com>
> Subject: [Histonet] Staining using a Plastic Sytem
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BANLkTikXBMVMdTC70GXe4_o3_N=ujYyOWw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
>
> Does anyone have experience embedding and staining in plastic? We have
> rabbit rotator cuff tendons that are being embedded in plastic. I am
> trying
> to figure out what the process is and which stains to use in order to
> visualize (and quantify) blood vessels and collagen deposition at the
> Bone
> (Humeral Head) - Tendon (Infraspinatus) interface.
>
> Thanks,
> Mahesh
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 12 Apr 2011 14:57:18 -0400
> From: "Evans, Andria B" <aevans3 <@t> lghealth.org>
> Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60 <@t> MAIL-AG-CLUSTER.lha.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Our lab is currently looking for a way to shorten our Biopsy
> processing program without compromising the patient specimen. We do
> have an issue with our GI's being very dry, which causes us to have to
> soak between each level taken and also causes a lot of chatter. Also
> we have a goal to do a run during the day to improve turn around time.
> Here is what our current protocol is....
>
> Formalin 1 min 37degrees
> Formalin 15 mins 37degrees
> 70 14 mins 40 degrees
> 95 14 mins 40 degrees
> 95 9 mins 40 degrees
> 100 9 mins 40 degrees
> 100 7 mins 40 degrees
> 100 4 mins 40 degrees
> Xylene 23 mins no heat
> Xylene 15 mins no heat
> Paraffin 20 mins 60 degrees
> Paraffin 18 mins 60 degrees
> Paraffin 10 mins 60 degrees
> Paraffin 0 mins 60 degrees
>
> All the steps are set on a fast mix setting. All of our biopsy
> specimens are put into sponges.
>
> Any feedback would be greatly appreciated.
>
> Andria B Evans HTL(ASCP)CM
> Lancaster General Hospital
> 555 North Duke Street
> Lancaster, PA 17604
> (717)544-5511 ext: 77329
> aevans3 <@t> lgheath.org<mailto:aevans3 <@t> lgheath.org>
> This email was sent securely from the LGHealth Email Service
>
> Confidentiality Notice: This e-mail message, including any
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> of intended recipient(s) and may contain confidential and
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>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 12 Apr 2011 12:27:49 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Milestone MW
> To: Histonet <@t> lists.utsouthwestern.edu, SHANE NELSON
> <nelsonrnch <@t> verizon.net>
> Message-ID: <809117.78546.qm <@t> web65715.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Model "KOS" by Milestone is capable of tissue processing,
> decalcification, special stains, fixation, gross hardening, and
> antigen retrieval.
> René J.
>
> --- On Tue, 4/12/11, SHANE NELSON <nelsonrnch <@t> verizon.net> wrote:
>
>
> From: SHANE NELSON <nelsonrnch <@t> verizon.net>
> Subject: [Histonet] Milestone MW
> To: Histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, April 12, 2011, 12:30 PM
>
>
> Margret,
> Which microwave would that be
>
> THANK YOU,
>
> PATTI RUBEN-NELSON H.T.(ASCP) LABORATORY CONSULTANT
> SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS
> 35-900 Bob Hope Drive
> Suite 275
> Rancho Mirage, Ca. 92270
> cell (909) 841-9761 / wk (760) 321-2500
> nelsonrnch <@t> verizon.net
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 12 Apr 2011 12:30:26 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Von kossa stain
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>, Dorothy LWebb
> <Dorothy.L.Webb <@t> HealthPartners.Com>
> Message-ID: <145774.98333.qm <@t> web65704.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> You can use any strong intensity microscope light. The stronger
> the intensity of the light the better, but the reduction has a
> cumulative effect so even not very strong intensity bulbs can be
> effective.
> René J.
>
> --- On Tue, 4/12/11, Webb, Dorothy L
> <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:
>
>
> From: Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com>
> Subject: [Histonet] Von kossa stain
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Date: Tuesday, April 12, 2011, 1:35 PM
>
>
> What is everyone using for their "light" when developing the silver in
> the VonKossa stain when you have no sunlight to use? We used to use a
> 60 watt lamp, but haven't done one for years and am bringing this
> stain back to our repetiore due to pathologist request. Thanks much!
>
> Dorothy Webb, HT (ASCP)
> Regions Histology Technical Supervisor
> 651-254-2962
>
>
>
>
> ________________________________
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to whom they
> are addressed. If you are not the intended recipient or the individual
> responsible for delivering the e-mail to the intended recipient,
> please be advised that you have received this e-mail in error and that
> any use, dissemination, forwarding, printing, or copying of this
> e-mail is strictly prohibited.
>
> If you have received this e-mail in error, please immediately notify
> the HealthPartners Support Center by telephone at (952) 967-6600. You
> will be reimbursed for reasonable costs incurred in notifying us.
> HealthPartners R001.0
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 12 Apr 2011 15:41:54 -0400
> From: Jack Ratliff <ratliffjack <@t> hotmail.com>
> Subject: RE: [Histonet] Von kossa stain
> To: <dorothy.l.webb <@t> healthpartners.com>, Histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BLU167-w542F31C988F23C9B15EFF9AEAB0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> I purchase a kit from Dorn and Hart Microedge and use sodium
> carbonate-formaldehyde solution to chemically develop.
>
> Stain in silver nitrate for 5 min in the dark, 3 fresh DI rinses for 1
> min each in the dark, then sodium carbonate-formaldehyde solution for
> 2 min in the dark, 2 fresh DI rinses (normal light) for 1 min each, 30
> seconds in Farmer's Diminisher (Sodium thiosulfate-potassium
> ferricyanide soluiton to stop reaction) and a running tap water rinse
> for 10 min.
> Jack
>
>
>> From: Dorothy.L.Webb <@t> HealthPartners.Com
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Tue, 12 Apr 2011 12:35:18 -0500
>> Subject: [Histonet] Von kossa stain
>>
>> What is everyone using for their "light" when developing the silver
>> in the VonKossa stain when you have no sunlight to use? We used to
>> use a 60 watt lamp, but haven't done one for years and am bringing
>> this stain back to our repetiore due to pathologist request. Thanks
>> much!
>>
>> Dorothy Webb, HT (ASCP)
>> Regions Histology Technical Supervisor
>> 651-254-2962
>>
>>
>>
>>
>> ________________________________
>> This e-mail and any files transmitted with it are confidential and
>> are intended solely for the use of the individual or entity to whom
>> they are addressed. If you are not the intended recipient or the
>> individual responsible for delivering the e-mail to the intended
>> recipient, please be advised that you have received this e-mail in
>> error and that any use, dissemination, forwarding, printing, or
>> copying of this e-mail is strictly prohibited.
>>
>> If you have received this e-mail in error, please immediately notify
>> the HealthPartners Support Center by telephone at (952) 967-6600. You
>> will be reimbursed for reasonable costs incurred in notifying us.
>> HealthPartners R001.0
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 12
> Date: Tue, 12 Apr 2011 23:11:35 +0000
> From: Tony Henwood <AnthonyH <@t> chw.edu.au>
> Subject: RE: [Histonet] Counterstain for dual chromogenic
> To: "'Eva Permaul'" <eca9 <@t> georgetown.edu>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E925 <@t> xmdb02.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Try ethyl green:
> 1. 0.1N Acetic Acid
> Add 6ml of glacial acetic acid to 1000 ml of distilled water. 2.
> 0.1N Sodium Acetate
> Add 4.102 g of sodium acetate to 500 ml of distilled water
> 3. pH 4.2 buffer
> Add 755 ml of 0.1N acetic acid to 264 ml of 0.1N Sodium Acetate and
> adjust the pH to 4.2 with 1M NaOH
> 4. Ethyl Green Solution
> To 100ml of buffer add 2g Ethyl Green (CI 42590)
> Can be stored at room temp. If staining turns bluish than the
> solution has started to deteriorate.
>
> Stain for 5 minutes. Do not rinse in water for too long, it tends to
> extract the green staining
>
> Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),
> FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306
> Fax: 612 9845 3318 the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva
> Permaul
> Sent: Wednesday, 13 April 2011 1:37 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Counterstain for dual chromogenic
>
> Good morning,
> I have been attempting to do some dual chromogenic staining. I am
> using a mouse and a rabbit antibody. For the mouse antibody I am using
> an HRP-mouse secondary followed by AEC for visualization. For the
> rabbit antibody I am using a biotinylated rabbit secondary, followed
> by ABC-AP and vector blue. My problem is what to use as a
> counterstain. From everything I have read so far there isn't one that
> would work without having some problems. I was thinking of trying a
> very light Hematoxylin and see if it doesn't disrupt the vector blue
> visualization too much. Does anyone else have any suggestions?
> Thanks,
> Eva
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> *********************************************************************************
> This email and any files transmitted with it are confidential and
> intended solely for the use of the individual or entity to whom they
> are addressed. If you are not the intended recipient, please delete it
> and notify the sender.
>
> Views expressed in this message and any attachments are those of the
> individual sender, and are not necessarily the views of The Children's
> Hospital at Westmead
>
> This note also confirms that this email message has been virus scanned
> and although no computer viruses were detected, The Childrens Hospital
> at Westmead accepts no liability for any consequential damage
> resulting from email containing computer viruses.
>
> *********************************************************************************
>
>
>
> ------------------------------
>
> Message: 13
> Date: Tue, 12 Apr 2011 23:22:21 +0000
> From: Tony Henwood <AnthonyH <@t> chw.edu.au>
> Subject: RE: [Histonet] Von kossa stain
> To: "'Breeden, Sara'" <sbreeden <@t> nmda.nmsu.edu>, "Webb, Dorothy L"
> <Dorothy.L.Webb <@t> HealthPartners.Com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E95A <@t> xmdb02.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Interesting,
>
> Meloan & Puchtler (1985) have drawn our attention to Von Kossa's
> original work on this technique. He regarded only the yellow
> colouration of calcium deposits during early stages of the reaction as
> diagnostic for calcium phosphate and credited the blackening to
> organic matter. Further studies showed that bright light only causes
> the irreversible blackening of organic matter that masks the yellow
> silver phosphate. When the reaction is performed in subdued light,
> yellow to yellowish brown silver phosphate is visualised selectively.
> Silver carbonate dissolves in sodium thiosulphate and cannot be
> demonstrated with von Kossa's technique (Meloan & Puchtler 1985 J
> Histotechnol 8(1):11-13.).
>
> Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),
> FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306
> Fax: 612 9845 3318 the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Breeden, Sara
> Sent: Wednesday, 13 April 2011 3:53 AM
> To: Webb, Dorothy L; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Von kossa stain
>
> Funny you should ask... just last week I had two requests for a Von
> Kossa. That's when I found out that, despite the fact that we just
> moved into a brand new building with all the bells and whistles, there
> was not one single UV light in any of the many hoods that had been
> installed. Heaven forbid I could even find an incandescent bulb (we
> are a "green building"). So, I prepped my slides, put them in a clear
> glass Coplin jar and parked the jar on the hood of my car for an hour.
>
> Works like a charm. This all depends, naturally, on (1) if you even
> HAVE sunshine where you live; (2) how far a walk it is to the
> sunshine, and (3) whether you have a car hood on which to park the
> Coplin jar.
> Needless to say, out of the line of sight of curious onlookers with
> sticky fingers and no business wondering what that glass jar is...
> Nonetheless, it works just fine! May the Force be with you.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> *********************************************************************************
> This email and any files transmitted with it are confidential and
> intended solely for the use of the individual or entity to whom they
> are addressed. If you are not the intended recipient, please delete it
> and notify the sender.
>
> Views expressed in this message and any attachments are those of the
> individual sender, and are not necessarily the views of The Children's
> Hospital at Westmead
>
> This note also confirms that this email message has been virus scanned
> and although no computer viruses were detected, The Childrens Hospital
> at Westmead accepts no liability for any consequential damage
> resulting from email containing computer viruses.
>
> *********************************************************************************
>
>
>
> ------------------------------
>
> Message: 14
> Date: Tue, 12 Apr 2011 23:30:30 +0000
> From: Tony Henwood <AnthonyH <@t> chw.edu.au>
> Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor
> To: "'Evans, Andria B'" <aevans3 <@t> lghealth.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E981 <@t> xmdb02.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Increase the fixation time and make sure the air is out of the sponges
> (will stop formalin from getting to the tissue- a quick dunk of the
> cassette (with sponge and tissue) in alcohol and back into formalin
> will do the trick).
>
> Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),
> FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306
> Fax: 612 9845 3318 the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Evans,
> Andria B
> Sent: Wednesday, 13 April 2011 4:57 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
>
> Our lab is currently looking for a way to shorten our Biopsy
> processing program without compromising the patient specimen. We do
> have an issue with our GI's being very dry, which causes us to have to
> soak between each level taken and also causes a lot of chatter. Also
> we have a goal to do a run during the day to improve turn around time.
> Here is what our current protocol is....
>
> Formalin 1 min 37degrees
> Formalin 15 mins 37degrees
> 70 14 mins 40 degrees
> 95 14 mins 40 degrees
> 95 9 mins 40 degrees
> 100 9 mins 40 degrees
> 100 7 mins 40 degrees
> 100 4 mins 40 degrees
> Xylene 23 mins no heat
> Xylene 15 mins no heat
> Paraffin 20 mins 60 degrees
> Paraffin 18 mins 60 degrees
> Paraffin 10 mins 60 degrees
> Paraffin 0 mins 60 degrees
>
> All the steps are set on a fast mix setting. All of our biopsy
> specimens are put into sponges.
>
> Any feedback would be greatly appreciated.
>
> Andria B Evans HTL(ASCP)CM
> Lancaster General Hospital
> 555 North Duke Street
> Lancaster, PA 17604
> (717)544-5511 ext: 77329
> aevans3 <@t> lgheath.org<mailto:aevans3 <@t> lgheath.org>
> This email was sent securely from the LGHealth Email Service
>
> Confidentiality Notice: This e-mail message, including any
> attachments, is for the sole use of intended recipient(s) and may
> contain confidential and privileged information. Any unauthorized
> review, use, disclosure or distribution is prohibited. If you are not
> the intended recipient, please contact the sender by reply e-mail and
> destroy all copies of the original message.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> *********************************************************************************
> This email and any files transmitted with it are confidential and
> intended solely for the use of the individual or entity to whom they
> are addressed. If you are not the intended recipient, please delete it
> and notify the sender.
>
> Views expressed in this message and any attachments are those of the
> individual sender, and are not necessarily the views of The Children's
> Hospital at Westmead
>
> This note also confirms that this email message has been virus scanned
> and although no computer viruses were detected, The Childrens Hospital
> at Westmead accepts no liability for any consequential damage
> resulting from email containing computer viruses.
>
> *********************************************************************************
>
>
>
> ------------------------------
>
> Message: 15
> Date: Tue, 12 Apr 2011 17:58:36 -0600
> From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
> Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6
> Processor
> To: <anthonyh <@t> chw.edu.au>, <aevans3 <@t> lghealth.org>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY151-w119D7602D3EDDB2E17742F91AB0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> I would consider replacing the sponges. Tony is correct that you need
> to make sure the air is removed from the sponge to facilitate
> exchange, but with shortened processing times you will undoubtedly
> cause carry over from solution to solution. Yuo may want to have a
> small container of alcoholic formalin with sponges sitting on the
> gross table.
>
> Try using a nylon tissue bag to funnel filter your small biopsies. all
> your biopsy sample sizes can be placed into the bag and very tiny
> samples are retained. Last suggestion is to consider a folded filter
> paper method (we name it origami, I can provide directly if you want)
> that uses a quick four fold process w/ forceps at the gross bench to
> create a pocket for the tiny samples, has a single layer between the
> tissue/solution and is easy to open at embedding (without "popping" of
> tissue samples). Avoind the unnecessary carryover of the sponge if you
> can.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>
>
>> From: AnthonyH <@t> chw.edu.au
>> To: aevans3 <@t> lghealth.org; histonet <@t> lists.utsouthwestern.edu
>> Date: Tue, 12 Apr 2011 23:30:30 +0000
>> CC: Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6
>> Processor
>>
>> Increase the fixation time and make sure the air is out of the
>> sponges (will stop formalin from getting to the tissue- a quick dunk
>> of the cassette (with sponge and tissue) in alcohol and back into
>> formalin will do the trick).
>>
>> Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),
>> FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306
>> Fax: 612 9845 3318 the children's hospital at westmead
>> Cnr Hawkesbury Road and Hainsworth Street, Westmead
>> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>> Evans, Andria B
>> Sent: Wednesday, 13 April 2011 4:57 AM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
>>
>> Our lab is currently looking for a way to shorten our Biopsy
>> processing program without compromising the patient specimen. We do
>> have an issue with our GI's being very dry, which causes us to have
>> to soak between each level taken and also causes a lot of chatter.
>> Also we have a goal to do a run during the day to improve turn around
>> time. Here is what our current protocol is....
>>
>> Formalin 1 min 37degrees
>> Formalin 15 mins 37degrees
>> 70 14 mins 40 degrees
>> 95 14 mins 40 degrees
>> 95 9 mins 40 degrees
>> 100 9 mins 40 degrees
>> 100 7 mins 40 degrees
>> 100 4 mins 40 degrees
>> Xylene 23 mins no heat
>> Xylene 15 mins no heat
>> Paraffin 20 mins 60 degrees
>> Paraffin 18 mins 60 degrees
>> Paraffin 10 mins 60 degrees
>> Paraffin 0 mins 60 degrees
>>
>> All the steps are set on a fast mix setting. All of our biopsy
>> specimens are put into sponges.
>>
>> Any feedback would be greatly appreciated.
>>
>> Andria B Evans HTL(ASCP)CM
>> Lancaster General Hospital
>> 555 North Duke Street
>> Lancaster, PA 17604
>> (717)544-5511 ext: 77329
>> aevans3 <@t> lgheath.org<mailto:aevans3 <@t> lgheath.org>
>> This email was sent securely from the LGHealth Email Service
>>
>> Confidentiality Notice: This e-mail message, including any
>> attachments, is for the sole use of intended recipient(s) and may
>> contain confidential and privileged information. Any unauthorized
>> review, use, disclosure or distribution is prohibited. If you are
>> not the intended recipient, please contact the sender by reply e-mail
>> and destroy all copies of the original message.
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> *********************************************************************************
>> This email and any files transmitted with it are confidential and
>> intended solely for the use of the individual or entity to whom they
>> are addressed. If you are not the intended recipient, please delete
>> it and notify the sender.
>>
>> Views expressed in this message and any attachments are those of the
>> individual sender, and are not necessarily the views of The
>> Children's Hospital at Westmead
>>
>> This note also confirms that this email message has been virus
>> scanned and although no computer viruses were detected, The Childrens
>> Hospital at Westmead accepts no liability for any consequential
>> damage resulting from email containing computer viruses.
>
>>
>> *********************************************************************************
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 16
> Date: Wed, 13 Apr 2011 00:10:04 +0000
> From: Tony Henwood <AnthonyH <@t> chw.edu.au>
> Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6
> Processor
> To: "'WILLIAM DESALVO'" <wdesalvo.cac <@t> hotmail.com>,
> "aevans3 <@t> lghealth.org" <aevans3 <@t> lghealth.org>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E9D3 <@t> xmdb02.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> I agree
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> From: WILLIAM DESALVO [mailto:wdesalvo.cac <@t> hotmail.com]
> Sent: Wednesday, 13 April 2011 9:59 AM
> To: Tony Henwood; aevans3 <@t> lghealth.org; histonet
> Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6
> Processor
>
> I would consider replacing the sponges. Tony is correct that you need
> to make sure the air is removed from the sponge to facilitate
> exchange, but with shortened processing times you will undoubtedly
> cause carry over from solution to solution. Yuo may want to have a
> small container of alcoholic formalin with sponges sitting on the
> gross table.
>
> Try using a nylon tissue bag to funnel filter your small biopsies. all
> your biopsy sample sizes can be placed into the bag and very tiny
> samples are retained. Last suggestion is to consider a folded filter
> paper method (we name it origami, I can provide directly if you want)
> that uses a quick four fold process w/ forceps at the gross bench to
> create a pocket for the tiny samples, has a single layer between the
> tissue/solution and is easy to open at embedding (without "popping" of
> tissue samples). Avoind the unnecessary carryover of the sponge if you
> can.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>
>
>> From: AnthonyH <@t> chw.edu.au<mailto:AnthonyH <@t> chw.edu.au>
>> To: aevans3 <@t> lghealth.org<mailto:aevans3 <@t> lghealth.org>;
>> histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
>> Date: Tue, 12 Apr 2011 23:30:30 +0000
>> CC:
>> Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor
>>
>> Increase the fixation time and make sure the air is out of the
>> sponges (will stop formalin from getting to the tissue- a quick dunk
>> of the cassette (with sponge and tissue) in alcohol and back into
>> formalin will do the trick).
>>
>> Regards
>> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
>> Laboratory Manager & Senior Scientist
>> Tel: 612 9845 3306
>> Fax: 612 9845 3318
>> the children's hospital at westmead
>> Cnr Hawkesbury Road and Hainsworth Street, Westmead
>> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>>
>>
>> -----Original Message-----
>> From:
>> histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.utsouthwestern.edu>
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]<mailto:[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]>
>> On Behalf Of Evans, Andria B
>> Sent: Wednesday, 13 April 2011 4:57 AM
>> To:
>> histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
>> Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
>>
>> Our lab is currently looking for a way to shorten our Biopsy
>> processing program without compromising the patient specimen. We do
>> have an issue with our GI's being very dry, which causes us to have
>> to soak between each level taken and also causes a lot of chatter.
>> Also we have a goal to do a run during the day to improve turn around
>> time. Here is what our current protocol is....
>>
>> Formalin 1 min 37degrees
>> Formalin 15 mins 37degrees
>> 70 14 mins 40 degrees
>> 95 14 mins 40 degrees
>> 95 9 mins 40 degrees
>> 100 9 mins 40 degrees
>> 100 7 mins 40 degrees
>> 100 4 mins 40 degrees
>> Xylene 23 mins no heat
>> Xylene 15 mins no heat
>> Paraffin 20 mins 60 degrees
>> Paraffin 18 mins 60 degrees
>> Paraffin 10 mins 60 degrees
>> Paraffin 0 mins 60 degrees
>>
>> All the steps are set on a fast mix setting. All of our biopsy
>> specimens are put into sponges.
>>
>> Any feedback would be greatly appreciated.
>>
>> Andria B Evans HTL(ASCP)CM
>> Lancaster General Hospital
>> 555 North Duke Street
>> Lancaster, PA 17604
>> (717)544-5511 ext: 77329
>>
>> aevans3 <@t> lgheath.org<mailto:aevans3 <@t> lgheath.org<mailto:aevans3 <@t> lgheath.org%3cmailto:aevans3 <@t> lgheath.org>>
>> This email was sent securely from the LGHealth Email Service
>>
>> Confidentiality Notice:
>> This e-mail message, including any attachments, is for the sole use
>> of intended recipient(s) and may contain confidential and privileged
>> information.
>> Any unauthorized review, use, disclosure or distribution is
>> prohibited.
>> If you are not the intended recipient, please contact the sender by
>> reply e-mail and destroy all copies of the original message.
>>
>> _______________________________________________
>> Histonet mailing list
>>
>> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> *********************************************************************************
>> This email and any files transmitted with it are confidential and
>> intended solely for the use of the individual or entity to whom they
>> are addressed. If you are not the intended recipient, please delete
>> it and notify the sender.
>>
>> Views expressed in this message and any attachments are those of the
>> individual sender, and are not necessarily the views of The
>> Children's Hospital at Westmead
>>
>> This note also confirms that this email message has been virus
>> scanned and although no computer viruses were detected, The Childrens
>> Hospital at Westmead accepts no liability for any consequential
>> damage resulting from email containing computer viruses.
>
>>
>> *********************************************************************************
>>
>> _______________________________________________
>> Histonet mailing list
>>
>> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 17
> Date: Tue, 12 Apr 2011 19:25:30 -0500
> From: Mahesh Polavarapu <polavarapu.mahesh <@t> gmail.com>
> Subject: [Histonet] Update regarding question on Plastic Embedding
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BANLkTikk_-oe3rO1KbxXd5x4Jj0kiM6gAw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Looking for a protocol to visualize vascularization and collagen
> deposition
> at the bone-tendon interface of a rabbit rotator cuff embedded in a
> plastic
> system. Sections will be rather thick (~50um) b/c they are being made
> through a titanium anchor. Using MMA with a cold-curing resin,
> Technovit
> 9100. Thanks in advance!
>
> - Mahesh
>
>
> ------------------------------
>
> Message: 18
> Date: Tue, 12 Apr 2011 20:34:51 -0400
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: [Histonet] SP3 HER2 mAb
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4DA4B76A.7400.0077.1 <@t> harthosp.org>
> Content-Type: text/plain; charset=US-ASCII
>
> Anyone using the HER2 rabbit monoclonal antibody (clone SP3) ever see
> reactivity with skeletal muscle? Thank you.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
>
>
>
> ------------------------------
>
> Message: 19
> Date: Tue, 12 Apr 2011 23:14:30 -0400
> From: Eugenia Thomas <eugenia902d1 <@t> hotmail.com>
> Subject: [Histonet] FW: 1 H&E slide vs. 2
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <COL103-W59F151894F9360078648E8D5AA0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
>
>
>
> From: eugenia902d1 <@t> hotmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: 1 H&E slide vs. 2
> Date: Mon, 11 Apr 2011 13:06:59 -0400
>
>
>
>
> Good afternoon everyone,
> Does anyone know of an article or statistics discussing the impact
> (medical diagnosing) of cutting 2 H&E slides per block verses 1 for
> all routine work?
> Genia
>
> ------------------------------
>
> Message: 20
> Date: Wed, 13 Apr 2011 08:39:22 -0500
> From: <Barbara.Crill <@t> LPNT.net>
> Subject: [Histonet] Ventana ultra
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <7DA79EBDBD92BF408EF392413737878D3936B06565 <@t> NADCWPMSGCMS01.hca.corpad.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone use the Benchmark Ultra - care to share your comments?
>
> We are considering switching from the Benchmark XT to the Ultra but
> would like to hear from users about this instrument.
> Is there an increase in reagent cost?
>
> Can you really add more slides without adding time to the run?
>
>
> ANTOINETTE CRILL, MBA,CT(ASCP)
> TEAM LEADER ANATOMIC PATHOLOGY
> DANVILLE REGIONAL MEDICAL CENTER
> (O) 434.799.4470
> (F) 434.773.6806
> E-mail: barbara.crill <@t> LPNT.net<mailto:barbara.crill <@t> LPNT.net>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Wed, 13 Apr 2011 10:00:09 -0400
> From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
> Subject: RE: [Histonet] FW: 1 H&E slide vs. 2
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <4EBFF65383B74D49995298C4976D1D5E08020E55 <@t> LSRIEXCH1.lsmaster.lifespan.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> When I was in clinical histology (I'm in research now) the
> pathologists
> didn't even want us putting two sections on a slide, because even
> though
> they knew a second serial section wasn't going to show anything the
> first section didn't show, they felt ethically/legally bound to
> examine
> both sections if they were there, so they were investing twice the
> time
> and effort for the same return.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Eugenia
> Thomas
> Sent: Tuesday, April 12, 2011 11:15 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] FW: 1 H&E slide vs. 2
>
>
>
>
>
> From: eugenia902d1 <@t> hotmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: 1 H&E slide vs. 2
> Date: Mon, 11 Apr 2011 13:06:59 -0400
>
>
>
>
> Good afternoon everyone,
> Does anyone know of an article or statistics discussing the impact
> (medical diagnosing) of cutting 2 H&E slides per block verses 1 for
> all
> routine work?
> Genia
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>
>
> ------------------------------
>
> Message: 22
> Date: Wed, 13 Apr 2011 09:09:22 -0500
> From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
> Subject: RE: [Histonet] Ventana ultra
> To: <Barbara.Crill <@t> LPNT.net>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <F0B9FD4B28C80E43996FFD4408B198AE074E6D <@t> UWHC-MAIL3.uwhis.hosp.wisc.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Please "Reply All" as I'd like to hear feedback also.
>
> Thanks.
>
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Barbara.Crill <@t> LPNT.net
> Sent: Wednesday, April 13, 2011 8:39 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Ventana ultra
>
> Does anyone use the Benchmark Ultra - care to share your comments?
>
> We are considering switching from the Benchmark XT to the Ultra but
> would like to hear from users about this instrument.
> Is there an increase in reagent cost?
>
> Can you really add more slides without adding time to the run?
>
>
> ANTOINETTE CRILL, MBA,CT(ASCP)
> TEAM LEADER ANATOMIC PATHOLOGY
> DANVILLE REGIONAL MEDICAL CENTER
> (O) 434.799.4470
> (F) 434.773.6806
> E-mail: barbara.crill <@t> LPNT.net<mailto:barbara.crill <@t> LPNT.net>
>
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>
> ------------------------------
>
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> End of Histonet Digest, Vol 89, Issue 13
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