[Histonet] uh...

[Jennifer MacDonald]

We had the same problem years ago using Clear-rite.  It turned out to be 
the mounting medium was not compatible.  We use Clear-rite routinely and 
do not have problems with the eosin bleeding.  We use Permount or on our 
coverslipper we use Sakura brand.
Jennifer




<sgoebel <@t> mirnarx.com> 
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04/07/2011 02:09 PM

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[Histonet] uh...






So I just stained a group of slides all at the same time with the same
conditions.  About 40 of 150 the eosin looks like it is bleeding out of
the sections...this has never happened before?  What could be the cause
and how do I fix it?  Everything is as normal, but again I am being
forced to use the crappy stuff called clear rite.  Could this be the
cause of the bleeding?

Thanks

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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