From cananatacan <@t> live.com Fri Apr 1 01:40:17 2011 From: cananatacan <@t> live.com (canan atacan) Date: Fri Apr 1 01:40:22 2011 Subject: [Histonet] coverslipping Message-ID: hi, I have a problem about coverslipping tape film. Could you please inform me about usage of this product If you send instructions for use of this product I will be appreciated. Thanks alot From DKBoyd <@t> chs.net Fri Apr 1 06:49:10 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Apr 1 06:49:20 2011 Subject: [Histonet] Poll on B5 In-Reply-To: Message-ID: We do not use B5. We've used Z5 (an Anatech product) for at least 10 years. Great product, it is a formaldehyde, zinc and ethanol mixture. We fix our core biopsies for a minimum of 2 hours before decalcification and processing. The aspiration is placed in 10% formalin per usual procedure. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Pitts, Jaclyn S. (Jackie), HT(ASCP)" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/31/2011 04:25 PM To cc Subject [Histonet] Poll on B5 Hello all. I was asked by our director to try to come up with a list of places that do still use and do not still use B5. We are working hard on trying to get to be a mercury free facility and other than the B5 we use for bone marrows we are mercury free. So, if you all could be so kind as to send me a response that would be much appreciated. I would like to know what you currently use, if you used B5 in the past or if you are currently using B5 now, and hospital name. I would absolutely appreciate any and all comments and help you all can provide. Thank you! Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic, Rochester MN E-mail: pitts.jaclyn@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From akbitting <@t> geisinger.edu Fri Apr 1 08:42:09 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Apr 1 08:42:19 2011 Subject: [Histonet] Kappa/Lambda ISH on Ventana In-Reply-To: References: Message-ID: <4D959DF0.2B7F.00C9.1@geisinger.edu> Ventana can no longer give out protocols. Theses probes are ASR products and the FDA doesn't allow them to tell you the protocol. They can offer guidance if you have a protocol that doesn't work well, but they can't outright tell you a protocol. Another institution that runs Ventana probes is your best bet. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Sebree Linda A" 3/31/2011 7:09 PM >>> You should be able to get protocols directly from Ventana. I tried out their K and L ISH probes and they gave us the protocols. They also supplied us with the protocol we use for EBER ISH. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Wilkes Sent: Thursday, March 31, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH on Ventana Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwilkes@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From LSebree <@t> uwhealth.org Fri Apr 1 08:51:51 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Apr 1 08:51:56 2011 Subject: [Histonet] Kappa/Lambda ISH on Ventana In-Reply-To: <4D959DF0.2B7F.00C9.1@geisinger.edu> References: <4D959DF0.2B7F.00C9.1@geisinger.edu> Message-ID: I was informed of the ASR issue also. Back in the day, vendors, including VMS, did give out protocols for ASR probes but no longer. The best I can offer is that with the VMS EBER probe we use ISH Protease 2/12" with great results. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ________________________________ From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, April 01, 2011 8:42 AM To: Steven Wilkes; histonet@lists.utsouthwestern.edu; Sebree Linda A Subject: RE: [Histonet] Kappa/Lambda ISH on Ventana Ventana can no longer give out protocols. Theses probes are ASR products and the FDA doesn't allow them to tell you the protocol. They can offer guidance if you have a protocol that doesn't work well, but they can't outright tell you a protocol. Another institution that runs Ventana probes is your best bet. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Sebree Linda A" 3/31/2011 7:09 PM >>> You should be able to get protocols directly from Ventana. I tried out their K and L ISH probes and they gave us the protocols. They also supplied us with the protocol we use for EBER ISH. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Wilkes Sent: Thursday, March 31, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH on Ventana Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwilkes@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From youngir <@t> gmail.com Fri Apr 1 13:38:34 2011 From: youngir <@t> gmail.com (Ian Young) Date: Fri Apr 1 13:38:39 2011 Subject: [Histonet] Sad news from Nebraska Message-ID: Histonetters, Those living in Nebraska may want to read this article. http://www.histobob.com/?q=node/156 HistoBob.com From liz <@t> premierlab.com Fri Apr 1 13:47:47 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Apr 1 13:47:53 2011 Subject: [Histonet] Sad news from Nebraska In-Reply-To: Message-ID: That has to be an April Fool joke, got ya!!! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Young Sent: Friday, April 01, 2011 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sad news from Nebraska Histonetters, Those living in Nebraska may want to read this article. http://www.histobob.com/?q=node/156 HistoBob.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Apr 1 14:09:17 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Apr 1 14:09:20 2011 Subject: [Histonet] cryostat repair--thanks! Message-ID: Thanks to all who answered my email, I ended up calling Tech One Biomedical and they came right out. I recommend them to anyone who needs histology equipment repaired!! Emily ps. this is not an april fool's joke. pps. really! It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins From louisesaruk <@t> gmail.com Fri Apr 1 14:15:42 2011 From: louisesaruk <@t> gmail.com (Louise Link Saruk) Date: Fri Apr 1 14:15:44 2011 Subject: [Histonet] Reserve Mohs Histotech Wanted. Message-ID: A North Wilmington, Delaware dermatology practice owned by a board-certified dermatopathologist and Fellow of the American Society of Mohs Surgery seeks the services of an experienced, technically gifted and fast Mohs histotech. We have need of someone to call upon in addition to, or as a back-up to our current tech. Mohs surgeries are scheduled back to back once or twice monthly. A typical Mohs day would mean 10-15 cases. Usually these are on Mondays or Tuesdays. If you have the required level of skill and this kind of flexibility please reply. Louise Link Saruk Interim Chief Operating Officer Delaware Valley Dermatology Group 3411 Silverside Road Webster Building, Suite 107 Wilmington, Delaware 19810 302-478-8532 www.delawarevalleyderm.com From JMacDonald <@t> mtsac.edu Sat Apr 2 01:12:29 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sat Apr 2 01:12:34 2011 Subject: [Histonet] Alk phos in muscle Message-ID: According to the literature, alkaline phosphatase is seen in both regenerating and degenerating muscle fibers. How would one differentiate between the two. I have been unable to find the answer to this. Thank you, Jennifer MacDonald From shrivastavaanuradha <@t> hotmail.com Sat Apr 2 18:00:30 2011 From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava) Date: Sat Apr 2 18:00:41 2011 Subject: [Histonet] thanks Message-ID: Hi Histo Friends, Thank you very much for yr help for PAS stain. you guys r great. thanks again to every body. Anu Shrivastava From c_m_hernandezjr <@t> yahoo.com Sat Apr 2 21:18:24 2011 From: c_m_hernandezjr <@t> yahoo.com (Carlos Hernandez) Date: Sat Apr 2 21:18:28 2011 Subject: [Histonet] Histonet Message-ID: <310601.15699.qm@web31804.mail.mud.yahoo.com> Histonet, Are you where you at you want to be in life? Do you want to end up working at taco bell, Or do you want to end up owning it? I can make the latter happen The more you want to get something done, the less you call it work. Everything about you is about to change. http://x.co/UJTi Everything you have done thus far in life has lead up to this point, Make your decision. From bill501 <@t> mindspring.com Sun Apr 3 07:45:20 2011 From: bill501 <@t> mindspring.com (Bill B.) Date: Sun Apr 3 07:45:33 2011 Subject: [Histonet] Histotech needed immediately SE Missouri Message-ID: I lost my histotech this weekend and need an immediate full time replacement for basic surgical pathology histology and low volume frozen sections. Our lab is near Cape Girardeau. Contact -- ___________________________ Wm F Blank, MD Heartland Laboratory 221 S. Main St. Chaffee, MO 63740 573-794-9011 573-887-3632 573-887-3635 (FAX) From amosbrooks <@t> gmail.com Sun Apr 3 17:15:28 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Apr 3 17:15:33 2011 Subject: [Histonet] Alk phos in muscle Message-ID: Hi, This is just a guess, but would degenerating muscle have Cleaved Caspase 3 or Tunel reactivity? Amos Message: 5 Date: Fri, 1 Apr 2011 23:12:29 -0700 From: Jennifer MacDonald Subject: [Histonet] Alk phos in muscle To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" According to the literature, alkaline phosphatase is seen in both regenerating and degenerating muscle fibers. How would one differentiate between the two. I have been unable to find the answer to this. Thank you, Jennifer MacDonald From LSebree <@t> uwhealth.org Sun Apr 3 18:12:34 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Sun Apr 3 18:12:39 2011 Subject: [Histonet] Ventana ISH probe protocols clarification Message-ID: Hello Histonetters, I just wanted to clarify an earlier post of mine stating that our lab received protocols from Ventana for their ISH probes. MANY years ago, when demo'ing several of Ventana's ISH probes, they provided working protocols for us. Now that these same and other probes are ASR status, Ventana may no longer and does not provide protocols for their ASR products. I hope this clears up any confusion I may have caused. LInda Sebree From MSHERWOOD <@t> PARTNERS.ORG Mon Apr 4 09:58:42 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Apr 4 09:58:50 2011 Subject: [Histonet] Giemsa Stain for frozens Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB561C@PHSXMB30.partners.org> I'm curious what Giemsa stain method people are using for frozen sections? I have a simple Wright-Giemsa stain that I use for blood smears (using a 1 min. methanol fix, 30 second stain with Wright-Giemsa, 5 minute in Phosphate buffer and then rinse in dH20). The investigator sent me a method using a HEPES buffer. It is involved to make up the working buffer of HEPES. I would appreciate any input. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From cbarone <@t> NEMOURS.ORG Mon Apr 4 10:14:27 2011 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Apr 4 10:14:38 2011 Subject: [Histonet] RE: Histonet Digest, Vol 89, Issue 2 In-Reply-To: References: Message-ID: Satelite cells indicate regeneration. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, April 02, 2011 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 89, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Sad news from Nebraska (Ian Young) 2. RE: Sad news from Nebraska (Liz Chlipala) 3. cryostat repair--thanks! (Emily Sours) 4. Reserve Mohs Histotech Wanted. (Louise Link Saruk) 5. Alk phos in muscle (Jennifer MacDonald) ---------------------------------------------------------------------- Message: 1 Date: Fri, 1 Apr 2011 13:38:34 -0500 From: Ian Young Subject: [Histonet] Sad news from Nebraska To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Histonetters, Those living in Nebraska may want to read this article. http://www.histobob.com/?q=node/156 HistoBob.com ------------------------------ Message: 2 Date: Fri, 1 Apr 2011 12:47:47 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Sad news from Nebraska To: "Ian Young" , Message-ID: Content-Type: text/plain; charset="us-ascii" That has to be an April Fool joke, got ya!!! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Young Sent: Friday, April 01, 2011 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sad news from Nebraska Histonetters, Those living in Nebraska may want to read this article. http://www.histobob.com/?q=node/156 HistoBob.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 1 Apr 2011 15:09:17 -0400 From: Emily Sours Subject: [Histonet] cryostat repair--thanks! To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Thanks to all who answered my email, I ended up calling Tech One Biomedical and they came right out. I recommend them to anyone who needs histology equipment repaired!! Emily ps. this is not an april fool's joke. pps. really! It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ------------------------------ Message: 4 Date: Fri, 1 Apr 2011 15:15:42 -0400 From: Louise Link Saruk Subject: [Histonet] Reserve Mohs Histotech Wanted. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 A North Wilmington, Delaware dermatology practice owned by a board-certified dermatopathologist and Fellow of the American Society of Mohs Surgery seeks the services of an experienced, technically gifted and fast Mohs histotech. We have need of someone to call upon in addition to, or as a back-up to our current tech. Mohs surgeries are scheduled back to back once or twice monthly. A typical Mohs day would mean 10-15 cases. Usually these are on Mondays or Tuesdays. If you have the required level of skill and this kind of flexibility please reply. Louise Link Saruk Interim Chief Operating Officer Delaware Valley Dermatology Group 3411 Silverside Road Webster Building, Suite 107 Wilmington, Delaware 19810 302-478-8532 www.delawarevalleyderm.com ------------------------------ Message: 5 Date: Fri, 1 Apr 2011 23:12:29 -0700 From: Jennifer MacDonald Subject: [Histonet] Alk phos in muscle To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" According to the literature, alkaline phosphatase is seen in both regenerating and degenerating muscle fibers. How would one differentiate between the two. I have been unable to find the answer to this. Thank you, Jennifer MacDonald ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 89, Issue 2 *************************************** From rsrichmond <@t> gmail.com Mon Apr 4 13:06:39 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Apr 4 13:06:43 2011 Subject: [Histonet] Re: Giemsa Stain for frozens Message-ID: Peggy Sherwood with the Wellman Center for Photomedicine at the Massachusetts General Hospital asks: >>I'm curious what Giemsa stain method people are using for frozen sections? I have a simple Wright-Giemsa stain that I use for blood smears (using a 1 min. methanol fix, 30 second stain with Wright-Giemsa, 5 minute in Phosphate buffer and then rinse in dH20). The investigator sent me a method using a HEPES buffer. It is involved to make up the working buffer of HEPES.<< If it LOOKS good, it IS good, as Duke Ellington might have said. In surgical pathology most people use one of the two-part Romanovsky type stains - Diff-Quik or any of a number of satisfactory generic equivalents. This is a very simple stain to do. I would think it would be publishable, since the stain is well known and easily obtained. Bob Richmond Samurai Pathologist Knoxville TN From amber.mckenzie <@t> gastrodocs.net Mon Apr 4 13:13:53 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Apr 4 13:13:57 2011 Subject: [Histonet] job opening Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3703FE89F8@giamail2.Gia.com> Job Opening in Jackson, MS: If anyone wants to work nights as a bench tech embedding, cutting, staining, putting cases together and filing slides at a GI lab in Jackson, MS, you can fax me your resume at 601-326-3532. Thanks! From MSHERWOOD <@t> PARTNERS.ORG Mon Apr 4 13:15:54 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Apr 4 13:15:58 2011 Subject: [Histonet] Re: Giemsa Stain for frozens In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5628@PHSXMB30.partners.org> Actually, Bob, the method the investigator sent me was the Romanovsky Giemsa that uses HEPES buffer. But it requires preparing all the reagents from scratch and the HEPES alone is several steps. I would like to buy prepared reagents. Can you buy a Giemsa kit for frozens? Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, April 04, 2011 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Giemsa Stain for frozens Peggy Sherwood with the Wellman Center for Photomedicine at the Massachusetts General Hospital asks: >>I'm curious what Giemsa stain method people are using for frozen sections? I have a simple Wright-Giemsa stain that I use for blood smears (using a 1 min. methanol fix, 30 second stain with Wright-Giemsa, 5 minute in Phosphate buffer and then rinse in dH20). The investigator sent me a method using a HEPES buffer. It is involved to make up the working buffer of HEPES.<< If it LOOKS good, it IS good, as Duke Ellington might have said. In surgical pathology most people use one of the two-part Romanovsky type stains - Diff-Quik or any of a number of satisfactory generic equivalents. This is a very simple stain to do. I would think it would be publishable, since the stain is well known and easily obtained. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From kblack <@t> digestivehlth.com Mon Apr 4 13:21:38 2011 From: kblack <@t> digestivehlth.com (Konni Black) Date: Mon Apr 4 13:21:46 2011 Subject: [Histonet] HT or HTL job opportunity Message-ID: HT or HTL wanted for new histology lab in Portland, Oregon. This is a fulltime position with a flexible schedule. Great benefits and excellent pay. Must have 3 to 5 years experience. If you are interested, please contact me via e-mail, kblack@digestivehlth.com. Thank you, Konni Black From pkrichar <@t> gundluth.org Mon Apr 4 13:36:55 2011 From: pkrichar <@t> gundluth.org (pkrichar@gundluth.org) Date: Mon Apr 4 13:37:58 2011 Subject: [Histonet] Cutting stations In-Reply-To: Message-ID: Hi, we are looking at possibly doing some remodeling and would like to know how others have their cutting stations setup. Are the built in or ergonomic tables, what size, what you like what you don't like, etc. Pictures sent to my work email would be great. Thanks in advance Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org From emendez84 <@t> yahoo.com Tue Apr 5 07:24:55 2011 From: emendez84 <@t> yahoo.com (Eric Mendez) Date: Tue Apr 5 07:24:59 2011 Subject: [Histonet] TBS Shurwave Microwave Message-ID: <510539.54634.qm@web46303.mail.sp1.yahoo.com> Hi All, I am looking into getting a TBS Shurwave Microwave and was trying to get some thoughts on the machine. I will be looking into processing GI biopsies and was wondering if the turn around time is good, consistency, reliability? Thanks, -E From nelsonrnch <@t> verizon.net Tue Apr 5 08:44:08 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Tue Apr 5 08:44:12 2011 Subject: [Histonet] TBS MICROWAVE PROCESSOR Message-ID: <505793.86130.qm@web84308.mail.re1.yahoo.com> Eric, I currently use the TBS shur/wave processor. I have used it for 4 years now and in all honesty in the beginning it was a struggle to find the programs that would work for me. TBS tech support is not what we would say?great by any means (they sucked). They were in no way helpful to me in?giving me direction what so ever. But now I am satisfied with the results we are getting in our lab after many hours of trouble shooting on my own. Bx?time for my program is about 22 minutes, 1 mm is 33, 2mm is 1 hour 3 minutes. I found that?microwaved processed tissue reacts very?differantly to staining. For example H & E staining times are much longer than some protocols I have used.?If you would like to ask me specific questions just e-mail me. THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS 35-900 Bob Hope Drive Suite 275 Rancho Mirage, Ca.? 92270 cell (909) 841-9761 / wk (760) 321-2500 nelsonrnch@verizon.net From talulahgosh <@t> gmail.com Tue Apr 5 09:11:27 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Apr 5 09:11:34 2011 Subject: [Histonet] cryostat repair--thanks! In-Reply-To: <4D962457.20406@pathology.washington.edu> References: <4D962457.20406@pathology.washington.edu> Message-ID: The compressor fan was loose, so when the Tech One guy fixed it, instead of making a horrible noise when it turns off, it just rattles. Oh well, time for a new motor. Which leads me to another question, is there any reason I should call Lecia to replace this motor? The guy from Tech One Biomedical seemed to know his stuff, but my boss seems to think we should call the people who made the cryostat. Personally, I think that would be spending a lot more money for the same thing, but maybe I'm missing something. Any suggestions? By the way, the cryostat is from 2008 and isn't under warranty. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 1, 2011 at 3:15 PM, Victor Tobias < victor@pathology.washington.edu> wrote: > So what was the problem? > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > On 4/1/2011 12:09 PM, Emily Sours wrote: > >> Thanks to all who answered my email, I ended up calling Tech One >> Biomedical >> and they came right out. I recommend them to anyone who needs histology >> equipment repaired!! >> >> Emily >> ps. this is not an april fool's joke. >> pps. really! >> >> It has become almost a cliche to remark that nobody boasts of ignorance of >> literature, but it is socially acceptable to boast ignorance of science >> and >> proudly claim incompetence in mathematics. >> -Richard Dawkins >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From talulahgosh <@t> gmail.com Tue Apr 5 09:14:29 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Apr 5 09:14:34 2011 Subject: [Histonet] science for kids Message-ID: I'm going to visit my nieces this summer and they want to know what I do at work. Does anyone know of a good book or website that explains DNA and/or histology for 4 to 6 year olds? Just thought I'd throw it out there, since googling it would yield way more than I can deal with. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron From kaylee <@t> medwrench.com Tue Apr 5 09:19:19 2011 From: kaylee <@t> medwrench.com (Kaylee McCaffrey) Date: Tue Apr 5 09:19:31 2011 Subject: [Histonet] IRMA TRUpoint Blood Analysis System Message-ID: Anyone know where to by CC or BG cartridges for the IRMA TRUpoint Blood Analysis System? Kaylee McCaffrey MedWrench Product Manager From nicole <@t> dlcjax.com Tue Apr 5 09:22:16 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Apr 5 09:26:28 2011 Subject: [Histonet] clia reg on ventilation Message-ID: <2891.208.62.167.196.1302013336.squirrel@webmail.realpages.com> Can anyone help me find some kind of standard for ventilation in a pathology laboratory. I cant seem to find anything more then adequate ventilation. Ok well what definies adequate? Is there a set Clia standard or AHCA standard for ventilation in Flordia. Thank you for your help. Nicole From JWeems <@t> sjha.org Tue Apr 5 09:29:06 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Apr 5 09:29:15 2011 Subject: [Histonet] cryostat repair--thanks! In-Reply-To: References: <4D962457.20406@pathology.washington.edu> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F3C5C78@CHEXCMS10.one.ads.che.org> The Tech One guy would do the same thing for much cheaper. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, April 05, 2011 10:11 To: Victor Tobias; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat repair--thanks! The compressor fan was loose, so when the Tech One guy fixed it, instead of making a horrible noise when it turns off, it just rattles. Oh well, time for a new motor. Which leads me to another question, is there any reason I should call Lecia to replace this motor? The guy from Tech One Biomedical seemed to know his stuff, but my boss seems to think we should call the people who made the cryostat. Personally, I think that would be spending a lot more money for the same thing, but maybe I'm missing something. Any suggestions? By the way, the cryostat is from 2008 and isn't under warranty. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 1, 2011 at 3:15 PM, Victor Tobias < victor@pathology.washington.edu> wrote: > So what was the problem? > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > University of Washington Medical Center Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > On 4/1/2011 12:09 PM, Emily Sours wrote: > >> Thanks to all who answered my email, I ended up calling Tech One >> Biomedical and they came right out. I recommend them to anyone who >> needs histology equipment repaired!! >> >> Emily >> ps. this is not an april fool's joke. >> pps. really! >> >> It has become almost a cliche to remark that nobody boasts of >> ignorance of literature, but it is socially acceptable to boast >> ignorance of science and proudly claim incompetence in mathematics. >> -Richard Dawkins >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From JWeems <@t> sjha.org Tue Apr 5 09:32:47 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Apr 5 09:32:59 2011 Subject: [Histonet] clia reg on ventilation In-Reply-To: <2891.208.62.167.196.1302013336.squirrel@webmail.realpages.com> References: <2891.208.62.167.196.1302013336.squirrel@webmail.realpages.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F3C5C7D@CHEXCMS10.one.ads.che.org> Google OSHA regs for histology lab ventilation and all kinds of stuff appears! See if there is something that would help you there. Best, J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, April 05, 2011 10:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] clia reg on ventilation Can anyone help me find some kind of standard for ventilation in a pathology laboratory. I cant seem to find anything more then adequate ventilation. Ok well what definies adequate? Is there a set Clia standard or AHCA standard for ventilation in Flordia. Thank you for your help. Nicole _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From DSiena <@t> statlab.com Tue Apr 5 10:04:22 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Tue Apr 5 10:04:31 2011 Subject: [Histonet] clia reg on ventilation In-Reply-To: <2891.208.62.167.196.1302013336.squirrel@webmail.realpages.com> References: <2891.208.62.167.196.1302013336.squirrel@webmail.realpages.com> Message-ID: You may want to check out Dick Dapson's book on Laboratory Safety, there is a chapter on ventilation there. Dapson R.W. (2001) Safety in the laboratory, Chapter 6 in Theory and Practice of Histological Technique Ed by Bancroft J.D.& Gamble, M Elsevier Science Ltd Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010? x229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, April 05, 2011 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] clia reg on ventilation Can anyone help me find some kind of standard for ventilation in a pathology laboratory. I cant seem to find anything more then adequate ventilation. Ok well what definies adequate? Is there a set Clia standard or AHCA standard for ventilation in Flordia. Thank you for your help. Nicole _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From swarthen <@t> wellspan.org Tue Apr 5 10:14:38 2011 From: swarthen <@t> wellspan.org (Warthen, Sheila) Date: Tue Apr 5 10:15:25 2011 Subject: [Histonet] Tissue Safe High Vacuum biospecimens transfer system Message-ID: I received some information from our surgical suite on the TissueSafe High Vacuum Biospecimens transfer system. Is anyone in histo land familiar with this machine? No price was listed. This claims to eliminate the use of formalin from the surgical suite. This is an alternative of how specimens are delivered to the laboratory with out formalin from the surgical suite. Any information would be greatly appreciated. Thanks, Sheila CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From Mandy.Bell <@t> chomp.org Tue Apr 5 11:45:59 2011 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Tue Apr 5 11:46:15 2011 Subject: [Histonet] RUO antibodies Message-ID: I know this has been brought up before, but the question of whether or not we can bill for RUO antibodies has come up in my lab. I was under the impression that you could use the RUO antibody with a disclaimer, but could not bill for it. Our pathologists say we can bill for RUO antibodies as long as we have the disclaimer. Does anyone know which is correct, and where I can find the documentation to back it up? Thanks. Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail From algranth <@t> email.arizona.edu Tue Apr 5 12:49:14 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Apr 5 12:50:49 2011 Subject: [Histonet] science for kids In-Reply-To: References: Message-ID: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> Emily, NSH has a coloring book that explains histology. Andi Grantham On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > I'm going to visit my nieces this summer and they want to know what I do at > work. Does anyone know of a good book or website that explains DNA and/or > histology for 4 to 6 year olds? Just thought I'd throw it out there, since > googling it would yield way more than I can deal with. > > Emily > > A great book should leave you with many experiences, and slightly exhausted. > You should live several lives while reading it. > -William Styron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jsjurczak <@t> comcast.net Tue Apr 5 13:32:54 2011 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Tue Apr 5 13:32:58 2011 Subject: [Histonet] science for kids In-Reply-To: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> Message-ID: <1168990097.2191742.1302028374627.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Really? ----- Original Message ----- From: "Andrea L - Grantham (algranth)" Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 5, 2011 12:49:14 PM Subject: Re: [Histonet] science for kids Emily, NSH has a coloring book that explains histology. Andi Grantham On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > I'm going to visit my nieces this summer and they want to know what I do at > work. ?Does anyone know of a good book or website that explains DNA and/or > histology for 4 to 6 year olds? Just thought I'd throw it out there, since > googling it would yield way more than I can deal with. > > Emily > > A great book should leave you with many experiences, and slightly exhausted. > You should live several lives while reading it. > -William Styron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Apr 5 14:19:18 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Apr 5 14:19:24 2011 Subject: [Histonet] science for kids In-Reply-To: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> References: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> Message-ID: A coloring book?! I want that for myself!!! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 5, 2011 at 1:49 PM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > Emily, > > NSH has a coloring book that explains histology. > > > Andi Grantham > > > > On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > > > I'm going to visit my nieces this summer and they want to know what I do > at > > work. Does anyone know of a good book or website that explains DNA > and/or > > histology for 4 to 6 year olds? Just thought I'd throw it out there, > since > > googling it would yield way more than I can deal with. > > > > Emily > > > > A great book should leave you with many experiences, and slightly > exhausted. > > You should live several lives while reading it. > > -William Styron > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From relia1 <@t> earthlink.net Tue Apr 5 14:24:50 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 5 14:24:54 2011 Subject: [Histonet] science for kids In-Reply-To: References: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> Message-ID: Me TOO!!!! How do we get them? Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, April 05, 2011 3:19 PM To: Grantham, Andrea L - (algranth); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] science for kids A coloring book?! I want that for myself!!! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 5, 2011 at 1:49 PM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > Emily, > > NSH has a coloring book that explains histology. > > > Andi Grantham > > > > On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > > > I'm going to visit my nieces this summer and they want to know what > > I do > at > > work. Does anyone know of a good book or website that explains DNA > and/or > > histology for 4 to 6 year olds? Just thought I'd throw it out there, > since > > googling it would yield way more than I can deal with. > > > > Emily > > > > A great book should leave you with many experiences, and slightly > exhausted. > > You should live several lives while reading it. > > -William Styron > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Tue Apr 5 14:34:40 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Tue Apr 5 14:34:44 2011 Subject: [Histonet] A PRN Histotech Position in Charleston, SC Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A297AE8E@NADCWPMSGCMS03.hca.corpad.net> Good Afternoon, We just got approval to replace our PRN HT that moved into a FTE position. In fact, the position # is 8978 on the tridenthealthsystem.com site under careers. It is not guaranteed hours per week, but on an as needed basis. Lately we've needed someone a lot!!! Preferable hours would be starting between 4:30-5:00 am and work until the work is caught up. Keep checking the website and fortunately for me, I am leaving tomorrow morning to go to the mountains to do some fly fishin' and relaxing!!! We'll talk when I get back. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From nicole <@t> dlcjax.com Tue Apr 5 14:36:07 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Apr 5 14:36:20 2011 Subject: [Histonet] Ventilation Message-ID: <4274.208.62.167.196.1302032167.squirrel@webmail.realpages.com> Thank you Fellow Histo's. I got the answers I seek. Thanks for the link Kay that was very helpful. Hope all is well. Nicole From trathborne <@t> somerset-healthcare.com Tue Apr 5 14:26:53 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Apr 5 14:45:35 2011 Subject: [Histonet] science for kids In-Reply-To: Message-ID: Where on the NSH website would I find this? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Emily Sours Sent: Tuesday, April 05, 2011 3:19 PM To: Grantham, Andrea L - (algranth); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] science for kids A coloring book?! I want that for myself!!! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 5, 2011 at 1:49 PM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > Emily, > > NSH has a coloring book that explains histology. > > > Andi Grantham > > > > On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > > > I'm going to visit my nieces this summer and they want to know what I do > at > > work. Does anyone know of a good book or website that explains DNA > and/or > > histology for 4 to 6 year olds? Just thought I'd throw it out there, > since > > googling it would yield way more than I can deal with. > > > > Emily > > > > A great book should leave you with many experiences, and slightly > exhausted. > > You should live several lives while reading it. > > -William Styron > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From abeharry798 <@t> gmail.com Tue Apr 5 15:20:37 2011 From: abeharry798 <@t> gmail.com (Andrea) Date: Tue Apr 5 15:18:52 2011 Subject: [Histonet] Computerized order entry Message-ID: <4412CDED-E425-4AEA-861C-785F31463059@gmail.com> Hi all, Anyone out there using computerized order entry for their histology or cytology specimens using Meditech? From nicole <@t> dlcjax.com Tue Apr 5 15:44:14 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Apr 5 15:44:27 2011 Subject: [Histonet] link to OSHA reg for lab ventilation Message-ID: <4386.208.62.167.196.1302036254.squirrel@webmail.realpages.com> http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=standards&p_id=10106 link to lab standard including ventilation. From jstaruk <@t> masshistology.com Tue Apr 5 16:14:45 2011 From: jstaruk <@t> masshistology.com (jstaruk) Date: Tue Apr 5 16:14:33 2011 Subject: [Histonet] New England Job Offering Message-ID: <01f601cbf3d6$7d35a650$77a0f2f0$@masshistology.com> HISTOLOGIST - Mass Histology Service, Inc. is a busy, well established, rapidly growing, highly reputable private histopathology lab located in central MA. We are looking for an experienced histologist with a strong background in routine and special stains, frozen sections, immunohistochemistry and general histology procedures. Must be HT certified or eligible. Hours are somewhat flexible and salary is based on experience as well as quality of work. We offer great benefits and a pleasant work environment. This is NOT a production line "slide factory". Potential partnership available to the right person. Please e-mail your cover letter, resume and salary requirements to info@masshistology.com. No recruiters please Thank you _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From ccrowder <@t> vetmed.lsu.edu Tue Apr 5 16:42:46 2011 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Tue Apr 5 16:46:58 2011 Subject: [Histonet] What we do for a living Message-ID: Hi - The NSH has a terrific coloring book what explains what we do and is really fun. It's meant for kids but I used to give it to "newbies" in our area. It cost a dollar I think. But whatever price, it's worth it. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 From lpwenk <@t> sbcglobal.net Tue Apr 5 19:12:22 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Apr 5 19:12:29 2011 Subject: [Histonet] science for kids In-Reply-To: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> References: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> Message-ID: <0E10B43126514267A6982947EBC4C84E@HP2010> The NSH Histology coloring book can be found at: https://www.nshonline.org/eweb/dynamicpage.aspx?expires=yes&webcode=COEMerchandiseSearch If that doesn't work, go to www.nsh.org In the blue bar, towards the right, click on NSH Today In the box that shows up, click on Marketplace The coloring book is about the 5th one down. Price is $5 for non-NSH members, $3 for NSH members. Please realize that they are 4 and 6 years old. They will not understand cells or nuclei. They will not be able to understand that their skin is made up of small things, too small for them to see. And that each of these tiny things have tiny nuclei/brains. At that age, they can understand things they can "feel" or see, like bones (dinosaurs), the stomach (as in aches, but don't try to explain 20 feet of intestines that look like a big worm - they will take that image literally), eyes (they can see them), the heart (they can feel it). They will understand the brain as "their head" doing the thinking, not as a separate organ. Lungs are somewhat iffy for the 6 year old - they will understand breathing in and out, any maybe the concept of a balloon. Kidney, liver, spleen, etc. are definitely out. The Crayola website has some nice very simple drawings that can be printed out. http://www.crayola.com/free-coloring-pages/Human-Body-coloring-pages/ And for those wondering what to do for the "Bring Your Son/Kid to Work Day", the Crayola website has drawing for several ages - skeletons and organs for the younger kids; the cell, heart and eye for the older kids. The other option for your nieces is an anatomy apron. Google it. It's vinyl, with outlines of the organs, and colorful organs that velcro onto the apron, in the correct location. Just a thought. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Grantham, Andrea L - (algranth)" Sent: Tuesday, April 05, 2011 1:49 PM Cc: Subject: Re: [Histonet] science for kids > Emily, > > NSH has a coloring book that explains histology. > > > Andi Grantham > > > > On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > >> I'm going to visit my nieces this summer and they want to know what I do >> at >> work. Does anyone know of a good book or website that explains DNA >> and/or >> histology for 4 to 6 year olds? Just thought I'd throw it out there, >> since >> googling it would yield way more than I can deal with. >> >> Emily >> >> A great book should leave you with many experiences, and slightly >> exhausted. >> You should live several lives while reading it. >> -William Styron >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cfitz <@t> 007group.com Tue Apr 5 21:17:54 2011 From: cfitz <@t> 007group.com (Cathy) Date: Tue Apr 5 21:17:52 2011 Subject: [Histonet] Tissue Safe High Vacuum biospecimens transfer system In-Reply-To: References: Message-ID: <69F7769EE7684A90884675E4E238E331@your8ba846406f> TissueSAFE is a system for vacuum sealing fresh surgical specimens. Once the specimen is sealed into the plastic bag it is kept at 2-8 C until it can be and placed into 10% Formalin. We did a small trial a few months ago. The initial results showed that the morphology was virtually the same; the IHC that we performed was also very similar. In our trial we only used single pieces of tissue not entire specimens. I have no idea how well the system would work on larger tissues. The only other trials that we had heard of were from Italy and Ireland. Cathy _____ From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Warthen, Sheila Sent: Tuesday, April 05, 2011 8:15 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Safe High Vacuum biospecimens transfer system I received some information from our surgical suite on the TissueSafe High Vacuum Biospecimens transfer system. Is anyone in histo land familiar with this machine? No price was listed. This claims to eliminate the use of formalin from the surgical suite. This is an alternative of how specimens are delivered to the laboratory with out formalin from the surgical suite. Any information would be greatly appreciated. Thanks, Sheila CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1209 / Virus Database: 1500/3550 - Release Date: 04/04/11 From amitapandey <@t> torrentpharma.com Wed Apr 6 00:08:59 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Wed Apr 6 00:13:08 2011 Subject: [Histonet] Tissue Safe High Vacuum biospecimens transfer system In-Reply-To: <69F7769EE7684A90884675E4E238E331@your8ba846406f> References: <69F7769EE7684A90884675E4E238E331@your8ba846406f> Message-ID: Cathy, I got just curious about ..... how many days we can keep the tissue at 2-8C ? Amita From: "Cathy" To: "'Warthen, Sheila'" , Date: 06/04/11 07:49 AM Subject: RE: [Histonet] Tissue Safe High Vacuum biospecimens transfer system Sent by: histonet-bounces@lists.utsouthwestern.edu TissueSAFE is a system for vacuum sealing fresh surgical specimens. Once the specimen is sealed into the plastic bag it is kept at 2-8 C until it can be and placed into 10% Formalin. We did a small trial a few months ago. The initial results showed that the morphology was virtually the same; the IHC that we performed was also very similar. In our trial we only used single pieces of tissue not entire specimens. I have no idea how well the system would work on larger tissues. The only other trials that we had heard of were from Italy and Ireland. Cathy _____ From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Warthen, Sheila Sent: Tuesday, April 05, 2011 8:15 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Safe High Vacuum biospecimens transfer system I received some information from our surgical suite on the TissueSafe High Vacuum Biospecimens transfer system. Is anyone in histo land familiar with this machine? No price was listed. This claims to eliminate the use of formalin from the surgical suite. This is an alternative of how specimens are delivered to the laboratory with out formalin from the surgical suite. Any information would be greatly appreciated. Thanks, Sheila CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1209 / Virus Database: 1500/3550 - Release Date: 04/04/11 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Wed Apr 6 02:55:09 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Wed Apr 6 02:55:22 2011 Subject: [Histonet] science for kids In-Reply-To: References: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> Message-ID: Me too, even in the UK! Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: 05 April 2011 20:25 To: 'Emily Sours' Cc: 'Histonet' Subject: RE: [Histonet] science for kids Me TOO!!!! How do we get them? Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, April 05, 2011 3:19 PM To: Grantham, Andrea L - (algranth); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] science for kids A coloring book?! I want that for myself!!! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 5, 2011 at 1:49 PM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > Emily, > > NSH has a coloring book that explains histology. > > > Andi Grantham > > > > On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > > > I'm going to visit my nieces this summer and they want to know what > > I do > at > > work. Does anyone know of a good book or website that explains DNA > and/or > > histology for 4 to 6 year olds? Just thought I'd throw it out there, > since > > googling it would yield way more than I can deal with. > > > > Emily > > > > A great book should leave you with many experiences, and slightly > exhausted. > > You should live several lives while reading it. > > -William Styron > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JoelI <@t> mcclainlab.com Wed Apr 6 07:13:41 2011 From: JoelI <@t> mcclainlab.com (Joel Israel) Date: Wed Apr 6 07:03:19 2011 Subject: [Histonet] C3a ab on pig paraffin sections Message-ID: Does anyone have experience with C3a antibody on porcine tissue (paraffin)? I am looking for a vender for the antibody and of course a procedure that works. Thanks in advance. Joel R. Israel McClain Laboratories, LLC From trathborne <@t> somerset-healthcare.com Wed Apr 6 09:03:18 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Apr 6 09:03:54 2011 Subject: [Histonet] Tissue Safe High Vacuum biospecimens transfer system In-Reply-To: <69F7769EE7684A90884675E4E238E331@your8ba846406f> Message-ID: What size pieces were used? How then were they transported? Were they ever placed in a pneumatic tube? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cathy Sent: Tuesday, April 05, 2011 10:18 PM To: 'Warthen, Sheila'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Safe High Vacuum biospecimens transfer system TissueSAFE is a system for vacuum sealing fresh surgical specimens. Once the specimen is sealed into the plastic bag it is kept at 2-8 C until it can be and placed into 10% Formalin. We did a small trial a few months ago. The initial results showed that the morphology was virtually the same; the IHC that we performed was also very similar. In our trial we only used single pieces of tissue not entire specimens. I have no idea how well the system would work on larger tissues. The only other trials that we had heard of were from Italy and Ireland. Cathy _____ From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Warthen, Sheila Sent: Tuesday, April 05, 2011 8:15 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Safe High Vacuum biospecimens transfer system I received some information from our surgical suite on the TissueSafe High Vacuum Biospecimens transfer system. Is anyone in histo land familiar with this machine? No price was listed. This claims to eliminate the use of formalin from the surgical suite. This is an alternative of how specimens are delivered to the laboratory with out formalin from the surgical suite. Any information would be greatly appreciated. Thanks, Sheila CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1209 / Virus Database: 1500/3550 - Release Date: 04/04/11 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From AHutton <@t> dh.org Wed Apr 6 13:01:09 2011 From: AHutton <@t> dh.org (Hutton, Allison) Date: Wed Apr 6 13:03:16 2011 Subject: [Histonet] solvent recycler waste Message-ID: <38A56C4F4630D348A50B3720409270870E0FE333@dhmail.dhorg.org> I was curious if anyone who uses the CBG recycler has ever tested the waste (the accumulation under the unit) to find out the specific components in the waste. My question refers both to the paraffin waste from the xylene run and the liquid waste from the alcohol run. Also, how does everyone dipose of that waste. I know laws are different in every state, I am located in PA. Thanks is advance Allison From Ken_Marissael <@t> vwr.com Wed Apr 6 13:03:54 2011 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Wed Apr 6 13:03:59 2011 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 04/05/2011 and will not return until 04/07/2011. I will be unavailable and will have limited access to phone and e-mail. While I am away, please contact VWR Customer Service at 800-932-5000. From michelecarr10 <@t> yahoo.com Wed Apr 6 13:16:15 2011 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Wed Apr 6 13:16:18 2011 Subject: [Histonet] patient melanin pigment Message-ID: <529110.84027.qm@web120714.mail.ne1.yahoo.com> Hi, I was wondering if there were a way to remove a patients melanin pigment on a slide. Any helpful advice would be appreciated. Thanks, Michele Carr Medical Laboratory Services From liz <@t> premierlab.com Wed Apr 6 13:21:49 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Apr 6 13:21:52 2011 Subject: [Histonet] solvent recycler waste In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE333@dhmail.dhorg.org> Message-ID: Why would you want to test the waste? We test the solutions generated but not the waste. We dispose of the liquid waste like the rest of our waste, its hauled away by a licensed vendor. The paraffin waste is placed into the trash in the same container its collected in, but we have not had to dispose of one yet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Wednesday, April 06, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] solvent recycler waste I was curious if anyone who uses the CBG recycler has ever tested the waste (the accumulation under the unit) to find out the specific components in the waste. My question refers both to the paraffin waste from the xylene run and the liquid waste from the alcohol run. Also, how does everyone dipose of that waste. I know laws are different in every state, I am located in PA. Thanks is advance Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From campbellj <@t> muhlbauerlab.com Wed Apr 6 13:22:36 2011 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Wed Apr 6 13:22:40 2011 Subject: [Histonet] patient melanin pigment In-Reply-To: <529110.84027.qm@web120714.mail.ne1.yahoo.com> References: <529110.84027.qm@web120714.mail.ne1.yahoo.com> Message-ID: We do a Melanin Bleach stain. On Wed, Apr 6, 2011 at 2:16 PM, Michele Carr wrote: > Hi, I was wondering if there were a way to remove a patients melanin > pigment on > a slide. Any helpful advice would be appreciated. > Thanks, > Michele Carr > Medical Laboratory Services > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Apr 6 13:25:49 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Apr 6 13:26:09 2011 Subject: [Histonet] patient melanin pigment In-Reply-To: <529110.84027.qm@web120714.mail.ne1.yahoo.com> References: <529110.84027.qm@web120714.mail.ne1.yahoo.com> Message-ID: you could do a melanin bleach Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Carr [michelecarr10@yahoo.com] Sent: Wednesday, April 06, 2011 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] patient melanin pigment Hi, I was wondering if there were a way to remove a patients melanin pigment on a slide. Any helpful advice would be appreciated. Thanks, Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Apr 6 13:28:09 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Apr 6 13:28:12 2011 Subject: [Histonet] patient melanin pigment In-Reply-To: <529110.84027.qm@web120714.mail.ne1.yahoo.com> References: <529110.84027.qm@web120714.mail.ne1.yahoo.com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E477AC@nmdamailsvr.nmda.ad.nmsu.edu> Deparaffinize and hydrate to DH20. Place slides into 0.25% potassium permanganate for 30 min to 1 hour (visually inspect to measure level of melanin removed); wash in running tap H20 for 1 minute and place into 5% oxalic acid for 2-5 minutes until section becomes clear. Wash in H20 and stain with H&E. From LSebree <@t> uwhealth.org Wed Apr 6 13:45:59 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Apr 6 13:46:02 2011 Subject: [Histonet] patient melanin pigment In-Reply-To: <529110.84027.qm@web120714.mail.ne1.yahoo.com> References: <529110.84027.qm@web120714.mail.ne1.yahoo.com> Message-ID: Are you talking about staining with H&E, special stains or immunos? If its to stain with IHC you can use a chromogen other than DAB for your detection kit. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Carr Sent: Wednesday, April 06, 2011 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] patient melanin pigment Hi, I was wondering if there were a way to remove a patients melanin pigment on a slide. Any helpful advice would be appreciated. Thanks, Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkmarshall <@t> anthc.org Wed Apr 6 14:21:42 2011 From: kkmarshall <@t> anthc.org (Marshall, Kimberly K) Date: Wed Apr 6 14:21:54 2011 Subject: [Histonet] Medical Grade Microwave Message-ID: Hello Histo peeps Just wondering where other labs purchased "Medical Grade Microwave" from. I am told the kind you buy at wal-mart to do special stains is no longer acceptable. Any info on this would be appreciated. Thanks Kimberly From mpence <@t> grhs.net Wed Apr 6 16:35:49 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Apr 6 16:35:54 2011 Subject: [Histonet] Slides sent out for outside consult. Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974BA1@is-e2k3.grhs.net> How is everyone handling a case that you send out to an outside consultant as far as billing the patient for the consult. Right now if I send out a case they bill me back an 88321. I am being told that I can not pass this same 88321 on to the patient. Having the outside consultant do the billing is not an option. Thanks, Mike From JWeems <@t> sjha.org Wed Apr 6 16:42:20 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Apr 6 16:42:24 2011 Subject: [Histonet] RE: Slides sent out for outside consult. In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974BA1@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974BA1@is-e2k3.grhs.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F3C60C3@CHEXCMS10.one.ads.che.org> Sorry, that is true and we often have to pay for them ourselves. There is supposed to be a limit that if the pathologists go over their group will be responsible, but so far I haven't had to resort to that. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, April 06, 2011 17:36 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides sent out for outside consult. How is everyone handling a case that you send out to an outside consultant as far as billing the patient for the consult. Right now if I send out a case they bill me back an 88321. I am being told that I can not pass this same 88321 on to the patient. Having the outside consultant do the billing is not an option. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From cbarone <@t> NEMOURS.ORG Wed Apr 6 16:51:38 2011 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Apr 6 16:51:42 2011 Subject: [Histonet] Seeking Immuno vendor... Message-ID: I am actively seeking an Immunohistochemistry Vendor to display and distribute information on their products at a INBRE CORE Facilities Open House event, Friday April 22nd Christiana Care Hopital - Ammon Ctr. - Wilmington, Delaware. Event: Each Core is requesting a supporting Vendor to display 8:00 am to 3:30 pm next to the corresponding booth: Histotechnology Core Laboratory, Nemours, Alfred I. du Pont Hospital for Children - Department of Biomedical Research. Contact cbarone@nemours.org ...We will feature Muscle Histochemistry and Immunohistochemistry Services. Only one Vendor per Core can be represented Thank you. or Dr. Rebekah Helton (rrhelton@udel.edu ) Dr. Katia Sol-Church (ksolchur@nemours.org ) Ms. Terry Pedicone (tpedicon@nemours.org ) Everyone else...Please join us... at the event Info...at https://secure.dbi.udel.edu/registration/coreopenhouse/ Participating cores include: Bioinformatics Core Facility, Nemours Biomolecular Core Laboratory, Nemours Cell Science Core, Nemours Christiana Care Center for Outcomes Research, CCHS CTCR Core Facility, UD CTCR Helen F. Graham Cancer Center, CCHS DBI Bio-Imaging Center, DBI DBI Bio-Informatics Center, DBI Histotechnology Core Laboratory, Nemours Protein Characterization Facility, DBI Proteomics & Mass Spectrometry Core Facility, DBI UD Sequencing & Genotyping Center, UD From vavalos <@t> allergydermatology.com Wed Apr 6 16:54:25 2011 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Wed Apr 6 16:54:31 2011 Subject: [Histonet] Slides sent out for outside consult. In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974BA1@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974BA1@is-e2k3.grhs.net> Message-ID: <000001cbf4a5$322a3840$967ea8c0$@com> Our practice does not bill the patient. Before sending they sign a waiver giving us permission to send out. On this waiver it states that if this is something their insurance does not cover then they may revieve a bill for $-$ range. We have an agreement with our consulting lab that they will only accept what the individual insurance covers. Our patients were getting ridiculous bills for consulting and our office was getting angry phone calls. After talking to the lab they made this agreement. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, April 06, 2011 2:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides sent out for outside consult. How is everyone handling a case that you send out to an outside consultant as far as billing the patient for the consult. Right now if I send out a case they bill me back an 88321. I am being told that I can not pass this same 88321 on to the patient. Having the outside consultant do the billing is not an option. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Thu Apr 7 09:26:22 2011 From: tifei <@t> foxmail.com (=?ISO-8859-1?B?dGkgZmVp?=) Date: Thu Apr 7 09:26:50 2011 Subject: [Histonet] Unsubscribe Message-ID: Hi please remove me from the mailing list ------------------ Original ------------------ From: "Vanessa Avalos"; Date: Thu, Apr 7, 2011 05:54 AM To: "HISTONET LISTS"; Subject: RE: [Histonet] Slides sent out for outside consult. Our practice does not bill the patient. Before sending they sign a waiver giving us permission to send out. On this waiver it states that if this is something their insurance does not cover then they may revieve a bill for $-$ range. We have an agreement with our consulting lab that they will only accept what the individual insurance covers. Our patients were getting ridiculous bills for consulting and our office was getting angry phone calls. After talking to the lab they made this agreement. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, April 06, 2011 2:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides sent out for outside consult. How is everyone handling a case that you send out to an outside consultant as far as billing the patient for the consult. Right now if I send out a case they bill me back an 88321. I am being told that I can not pass this same 88321 on to the patient. Having the outside consultant do the billing is not an option. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Apr 7 11:59:34 2011 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Apr 7 11:59:38 2011 Subject: [Histonet] QC documentation Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43010F85105497@HPEMX3.HealthPartners.int> How does everyone handle their QC documentation on special stains and IHC? We currently print out the run information from our stainer(s) and have the tech initial for her QC and the pathologist sign after they review the slides. I am hoping that someone has a way of doing this electronically. I hope to learn of a better way to save some trees!!! Thank you ahead of time! Dorothy Webb, HT Regions Histology Technical Specialist ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From beth.villarreal <@t> novartis.com Thu Apr 7 12:35:25 2011 From: beth.villarreal <@t> novartis.com (Villarreal, Beth) Date: Thu Apr 7 12:35:32 2011 Subject: [Histonet] rabbit neutrophil/heterophil IHC In-Reply-To: <201104051702.p35H2sZJ027485@ch2ssmenov01.novartis.com> References: <201104051702.p35H2sZJ027485@ch2ssmenov01.novartis.com> Message-ID: Does anyone know of a good antibody to detect rabbit heterophils (neutrophils)? I'd love one that works for FFPE, but we can go frozen if necessary. Thanks in advance! Beth Villarreal Preclinical Safety, Discovery Pathology Novartis Institutes for BioMedicalResearch, Inc. 300 Technology Square Cambridge, MA 02139 USA Phone +1 617 8714725 Fax +1 617 8714931 beth.villarreal@novartis.com From victor <@t> pathology.washington.edu Thu Apr 7 13:14:39 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Apr 7 13:15:00 2011 Subject: [Histonet] QC documentation In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F85105497@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F85105497@HPEMX3.HealthPartners.int> Message-ID: <4D9DFF0F.4080805@pathology.washington.edu> Dorothy, I'm only going to address what we are doing for IHC. We have a custom worksheet that is printed from the LIS for each patient. We were totally manual until 3 weeks ago when we went live with the Bond. We are still using our custom worksheet for the techs and pathologists. Boxes for them to check off and write comments. We just modified our IHC resulting tool and added fields for the pathologist to electronically record the QC. The manual QC paperwork gets returned to the lab and can be used for CAP and we can generate an electronic report also. Hopefully we'll get more paperless with time. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 4/7/2011 9:59 AM, Webb, Dorothy L wrote: > How does everyone handle their QC documentation on special stains and IHC? We currently print out the run information from our stainer(s) and have the tech initial for her QC and the pathologist sign after they review the slides. I am hoping that someone has a way of doing this electronically. I hope to learn of a better way to save some trees!!! Thank you ahead of time! > > Dorothy Webb, HT > Regions Histology Technical Specialist > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Thu Apr 7 13:22:16 2011 From: bill501 <@t> mindspring.com (Bill B.) Date: Thu Apr 7 13:22:27 2011 Subject: [Histonet] Histotech needed immediately SE Missouri In-Reply-To: References: Message-ID: Thanks all who responded. Problem solved. At 7:45 AM -0500 4/3/11, Bill B. wrote: >I lost my histotech this weekend and need an immediate full time replacement for basic surgical pathology histology and low volume frozen sections. > >Our lab is near Cape Girardeau. > >Contact >-- >___________________________ >Wm F Blank, MD >Heartland Laboratory >221 S. Main St. >Chaffee, MO 63740 >573-794-9011 >573-887-3632 >573-887-3635 (FAX) From JEllin <@t> yumaregional.org Thu Apr 7 13:32:19 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Apr 7 13:32:25 2011 Subject: [Histonet] QC documentation In-Reply-To: <4D9DFF0F.4080805@pathology.washington.edu> References: <65365F35C0F2EF4D846EC3CA73E49C43010F85105497@HPEMX3.HealthPartners.int> <4D9DFF0F.4080805@pathology.washington.edu> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D6B05@EXCHANGECLUSTER.yumaregional.local> We currently use our LIS to support this issue,, the tech are able to go into the case and make comments about controls, staining, technical work etc. There are places for comment section, etc. I also use the LIS to document Fixation time for specimens. Multiple specimens are an issue, but we are able to document that as well. I then use the LIS to drill down the data and get me what I want. I still am using paper work, since the Pathologist needs to sign off. But the reports are attached and reviewed by the Pathologist. This also allows us to trend issues we are having within the Department. I like it a lot. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, April 07, 2011 11:15 AM To: Webb, Dorothy L Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] QC documentation Dorothy, I'm only going to address what we are doing for IHC. We have a custom worksheet that is printed from the LIS for each patient. We were totally manual until 3 weeks ago when we went live with the Bond. We are still using our custom worksheet for the techs and pathologists. Boxes for them to check off and write comments. We just modified our IHC resulting tool and added fields for the pathologist to electronically record the QC. The manual QC paperwork gets returned to the lab and can be used for CAP and we can generate an electronic report also. Hopefully we'll get more paperless with time. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 4/7/2011 9:59 AM, Webb, Dorothy L wrote: > How does everyone handle their QC documentation on special stains and IHC? We currently print out the run information from our stainer(s) and have the tech initial for her QC and the pathologist sign after they review the slides. I am hoping that someone has a way of doing this electronically. I hope to learn of a better way to save some trees!!! Thank you ahead of time! > > Dorothy Webb, HT > Regions Histology Technical Specialist > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From trathborne <@t> somerset-healthcare.com Thu Apr 7 13:36:54 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Apr 7 14:01:01 2011 Subject: [Histonet] QC documentation In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8035D6B05@EXCHANGECLUSTER.yumaregional.local> Message-ID: What LIS are you using? This sounds great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jesus Ellin Sent: Thursday, April 07, 2011 2:32 PM To: Victor Tobias; Webb, Dorothy L Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] QC documentation We currently use our LIS to support this issue,, the tech are able to go into the case and make comments about controls, staining, technical work etc. There are places for comment section, etc. I also use the LIS to document Fixation time for specimens. Multiple specimens are an issue, but we are able to document that as well. I then use the LIS to drill down the data and get me what I want. I still am using paper work, since the Pathologist needs to sign off. But the reports are attached and reviewed by the Pathologist. This also allows us to trend issues we are having within the Department. I like it a lot. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, April 07, 2011 11:15 AM To: Webb, Dorothy L Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] QC documentation Dorothy, I'm only going to address what we are doing for IHC. We have a custom worksheet that is printed from the LIS for each patient. We were totally manual until 3 weeks ago when we went live with the Bond. We are still using our custom worksheet for the techs and pathologists. Boxes for them to check off and write comments. We just modified our IHC resulting tool and added fields for the pathologist to electronically record the QC. The manual QC paperwork gets returned to the lab and can be used for CAP and we can generate an electronic report also. Hopefully we'll get more paperless with time. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 4/7/2011 9:59 AM, Webb, Dorothy L wrote: > How does everyone handle their QC documentation on special stains and IHC? We currently print out the run information from our stainer(s) and have the tech initial for her QC and the pathologist sign after they review the slides. I am hoping that someone has a way of doing this electronically. I hope to learn of a better way to save some trees!!! Thank you ahead of time! > > Dorothy Webb, HT > Regions Histology Technical Specialist > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From sgoebel <@t> mirnarx.com Thu Apr 7 16:06:17 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Apr 7 16:06:20 2011 Subject: [Histonet] uh... Message-ID: So I just stained a group of slides all at the same time with the same conditions. About 40 of 150 the eosin looks like it is bleeding out of the sections...this has never happened before? What could be the cause and how do I fix it? Everything is as normal, but again I am being forced to use the crappy stuff called clear rite. Could this be the cause of the bleeding? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From chapcl <@t> yahoo.com Thu Apr 7 16:11:55 2011 From: chapcl <@t> yahoo.com (William Chappell) Date: Thu Apr 7 16:12:06 2011 Subject: [Histonet] uh... In-Reply-To: References: Message-ID: Sounds like your dehydrating alcohol is not dehydrating completely. I would switch out the 100%'s and the clearite. Either they got "wet" during the course of the day or someone made an oops when changing the stain line. Hope that helps. Will Sent from my iPhone On Apr 7, 2011, at 2:06 PM, wrote: > So I just stained a group of slides all at the same time with the same > conditions. About 40 of 150 the eosin looks like it is bleeding out of > the sections...this has never happened before? What could be the cause > and how do I fix it? Everything is as normal, but again I am being > forced to use the crappy stuff called clear rite. Could this be the > cause of the bleeding? > > Thanks > > > > Sarah Goebel, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Thu Apr 7 16:23:11 2011 From: chapcl <@t> yahoo.com (William Chappell) Date: Thu Apr 7 16:23:13 2011 Subject: [Histonet] uh... In-Reply-To: Message-ID: <514817.33759.qm@web30604.mail.mud.yahoo.com> Oh yes, how to fix it. Hydrate through clearite, 100% ETOH, 95% ETOH, and water. Spend an extra 5 mins in running tap water -- that should wash out most of the eosin. Then counterstain again with eosin. Dehydrate with the clean ETOHs and Clearite. That should do it. Another possibility I just thought of, I never used clearite for coverslipping, even in labs that primarily used clearite -- I always finished up in Xylene. Will --- On Thu, 4/7/11, William Chappell wrote: > From: William Chappell > Subject: Re: [Histonet] uh... > To: "" > Cc: "" > Date: Thursday, April 7, 2011, 2:11 PM > Sounds like your dehydrating alcohol > is not dehydrating completely. I would switch out the 100%'s > and the clearite. Either they got "wet" during the course of > the day or someone made an oops when changing the stain > line. > > Hope that helps. > > Will > > Sent from my iPhone > > On Apr 7, 2011, at 2:06 PM, > wrote: > > > So I just stained a group of slides all at the same > time with the same > > conditions.? About 40 of 150 the eosin looks like > it is bleeding out of > > the sections...this has never happened before?? > What could be the cause > > and how do I fix it?? Everything is as normal, > but again I am being > > forced to use the crappy stuff called clear > rite.? Could this be the > > cause of the bleeding? > > > > Thanks > > > > > > > > Sarah Goebel, BA, HT(ASCP) > > > > Histotechnologist > > > > Mirna Therapeutics > > > > 2150 Woodward Street > > > > Suite 100 > > > > Austin, Texas? 78744 > > > > (512)901-0900 ext. 6912 > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ruppert.amysue <@t> marshfieldclinic.org Thu Apr 7 21:48:01 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Thu Apr 7 21:48:07 2011 Subject: [Histonet] RE%3A Verhoeff/Masson%27s Stain&In-Reply-To=9BF995BC0E47744E9673A4148 Message-ID: <201104080248.p382m3DY004941@mailhost2.mfldclin.edu> Hello John, Just reading through some histonet archives and found your Elastic- Masson issue. Some notes on the elastic masson stain.... The fibers should darken when the slides go into running water after the 2% ferric chloride. So the color before the ferric chloride will not be the same intense color as after the running water. But one very important point is that with this stain, you should actually UNDER differentiate the slides in the 2% ferric chloride because the solutions in the masson part of the procedure will also differeniate out the fibers. And so you could actually wipe out the fiber staining after going through the entire procedure. In our lab, the technique is to do only 2-3 dips in the 2% ferric chloride (yes, this will seem like not enough time in the ferric chloride, but remember, the masson solutions will also differentiate the slides) and then procede with the rest of the staining and then you should be seeing the fibers. Also, do not be afraid to try to contact PolyScientific, they are a very helpful company. Good luck. amysue ruppert ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From JMacDonald <@t> mtsac.edu Fri Apr 8 00:36:57 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Apr 8 00:37:05 2011 Subject: [Histonet] uh... In-Reply-To: Message-ID: We had the same problem years ago using Clear-rite. It turned out to be the mounting medium was not compatible. We use Clear-rite routinely and do not have problems with the eosin bleeding. We use Permount or on our coverslipper we use Sakura brand. Jennifer Sent by: histonet-bounces@lists.utsouthwestern.edu 04/07/2011 02:09 PM To cc Subject [Histonet] uh... So I just stained a group of slides all at the same time with the same conditions. About 40 of 150 the eosin looks like it is bleeding out of the sections...this has never happened before? What could be the cause and how do I fix it? Everything is as normal, but again I am being forced to use the crappy stuff called clear rite. Could this be the cause of the bleeding? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Apr 8 08:00:49 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Apr 8 08:00:59 2011 Subject: [Histonet] Controls for IPs In-Reply-To: <53FC421CC200C5429929EDE6C3676F30014EC205@msgebe34> References: <53FC421CC200C5429929EDE6C3676F30014EC205@msgebe34> Message-ID: <4D9ECEC1.7400.0077.1@harthosp.org> This response is late due to the overwhelming time commitments of NCAA Basketball Tournaments. The only "multi-tissue" control we use is for HER2 protein overexpression. In my opinion, "multi-tissue" controls are completely unnecessary for every-day use for the majority of IHC tests that we do. They are expensive and time-consuming to prepare and, as you pointed out, consume valuable tissue. "Multi-tissue" controls should be used during primary antibody optimization and validation studies; they are not needed for routine testing. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Bauer, Karen L." 3/8/2011 10:16 AM >>> Hello, It seems we go back and forth between multi-tissue control blocks and single tissue control blocks when it comes to immunostaining. In the past we used single blocks and still continue to use some for the IPs that are rarely used. Then, switched to creating multi-tissue blocks for ease of use. It's so nice to cut control slides ahead of time and just pull them out and add our patient tissue. But... we find that the multi-tissue blocks are using up hard to find tissue. For example, we have a melanoma skin in one of the MTBs for the HMB45 and Melan A stains, but that MTB is used more for the CK7, CK20 and TTF-1 stains. Basically, that melanoma skin is just being wasted. I'm considering going back to the single block controls and was wondering what other labs are doing. What is the concensus in HistoLand in regards to IP control blocks/tissues? Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System bauer.karen@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LouroP <@t> Princeton.Huntingdon.com Fri Apr 8 08:51:59 2011 From: LouroP <@t> Princeton.Huntingdon.com (Louro, Pedro) Date: Fri Apr 8 08:52:10 2011 Subject: [Histonet] CD4, CD8 on Monkey tissue Message-ID: HI everyone, I wanted to see if anyone out there has had any luck using any CD4 and CD8 antibodies on FFPE monkey tissue. I have used a couple of Ab's that work great on human tissue but no luck with monkey tissue. Any information will be greatly appreciated. Thanks, Pedro Louro, MBA, QIHC IHC Technologist HLS CRO ******************************************************************************************** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ******************************************************************************************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. 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Maybe no one will notice =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From kenneth.metzger <@t> aruplab.com Fri Apr 8 09:58:00 2011 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Fri Apr 8 09:58:07 2011 Subject: [Histonet] Control Tissue Message-ID: Does anyone have or know where I can get control tissue for the MYCN (2p 24.1) FISH probe assay, an amplified or positive control? Thanks Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From Marcia_Gaiser <@t> ssmhc.com Fri Apr 8 09:58:20 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Fri Apr 8 09:58:29 2011 Subject: [Histonet] HT Position in Oklahoma City Message-ID: <728F817C02110E498D803A7C3B0C6248068B1F5B43@S009-APEXM06.ds.ad.ssmhc.com> St. Anthony Hospital currently has an excellent opportunity for an experienced Histology Technician. This position requires Certification as an HT or HLT ? or ? experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.com>>>, Ad # 10762, or contact Anna King for additional information at (405) 272-6105. Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From Herrick.James <@t> mayo.edu Fri Apr 8 12:09:45 2011 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Fri Apr 8 12:10:44 2011 Subject: [Histonet] Movat's Pentachrome Question Message-ID: <7267A64D75F58241B577876D8A885631038FE512@msgebe41> Happy Friday everyone!! I am trying to find a good Modified Movat's pentachrome stain protocol for plastic embedded iliac arteries and also bone tissue (animal model). Everything I have been able to find so far, is for paraffin embedded tissue and a mile long. There may not be a fix for the length of the protocol but if anyone would happen to have a technique that works well with plastic, I would greatly appreciate it. Thank you much. Have a great weekend!! Jim From jengirl1014 <@t> yahoo.com Fri Apr 8 13:29:46 2011 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Apr 8 13:29:49 2011 Subject: [Histonet] coverslippers Message-ID: <464701.128.qm@web125405.mail.ne1.yahoo.com> Hi Everyone! Our?small core is looking into getting a coverslipper and I was wondering the following things: 1) Which company did you get yours from? 2) Is there a difference between glass vs. plastic? (thinking of allowing people who do IHC/IMF to use it too) 3) What are the pros and cons of your machine? The world of coverslippers is huge and I'm just getting into the automation---FINALLY!! Would love to hear from the experienced! Thanks so much for all of your help in advance!? I truly appreciate it! Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD? 21205 phone:? 410-614-0131 ?????????????? 410-955-9688 fax:???????? 410-955-9677 cell:??????? 443-631-6361 e-mail:? jsipes1@jhmi.edu From rsrichmond <@t> gmail.com Fri Apr 8 14:11:37 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Apr 8 14:11:42 2011 Subject: [Histonet] Re: QC documentation Message-ID: Let me add the dyspeptic observation that, in the many pathology services I've worked in in the last 30 years - the more QC paperwork I have to do, the worse the slides. I've never seen it done, but I think the only effective QC program in histology would be daily review of selected slides by a pathologist and a senior histotechnologist, with documentation of what problems were observed and what got done about them. I'm sure neither Six Sigma nor LEAN (whatever they may be) would approve of this Edwards Deming approach. Bob Richmond Samurai Pathologist Knoxville TN From amkmadden <@t> gmail.com Fri Apr 8 14:11:40 2011 From: amkmadden <@t> gmail.com (Amanda Madden) Date: Fri Apr 8 14:12:04 2011 Subject: [Histonet] Leica Cryostat Service Recommendations Message-ID: Hello Histonetters!!! I am writing today to ask about the necessity of having cryostats serviced regularly. We have a Leica CM 3050S that is about 2 years old now. I have been looking far and wide for recommendations about cryostat maintenance, but haven't found much information. Do you think it is necessary to purchase a service contract that includes having someone come out regularly to check on the equipment? We love our Leica rep, but I haven't wanted to contact him about this until I have a better idea of what other labs are doing. I should mention that ours is a very small lab, with sectioning time averaging only about 5 hours per week. Thanks for any advice! Amanda From LSetlak <@t> childrensmemorial.org Fri Apr 8 14:18:40 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Fri Apr 8 14:18:46 2011 Subject: [Histonet] coverslippers In-Reply-To: <464701.128.qm@web125405.mail.ne1.yahoo.com> References: <464701.128.qm@web125405.mail.ne1.yahoo.com> Message-ID: <7111DB39D045004C9CF29E79C71B28BC101D507908@CMHEXCC01MBX.childrensmemorial.org> We have the tape coverslipper from Sakura and have had it for years.... we love it. Yo need to make sure when you change the tape that it is allowed to sit out of the package for a little while. We put one on the machine and open a new one and let it sit out- but I think overnight is fine. It's very low maintenance. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes Sent: Friday, April 08, 2011 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslippers Hi Everyone! Our?small core is looking into getting a coverslipper and I was wondering the following things: 1) Which company did you get yours from? 2) Is there a difference between glass vs. plastic? (thinking of allowing people who do IHC/IMF to use it too) 3) What are the pros and cons of your machine? The world of coverslippers is huge and I'm just getting into the automation---FINALLY!! Would love to hear from the experienced! Thanks so much for all of your help in advance!? I truly appreciate it! Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD? 21205 phone:? 410-614-0131 ?????????????? 410-955-9688 fax:???????? 410-955-9677 cell:??????? 443-631-6361 e-mail:? jsipes1@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Apr 8 14:24:05 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Apr 8 14:24:09 2011 Subject: [Histonet] Leica Cryostat Service Recommendations In-Reply-To: References: Message-ID: We have the same cryostat, three years old and this is the first time anything has broken on it. The total cost for everything will be about $600 which is waaaay cheaper than a $4000 service contract (for one year!). Also we use our cryostat almost every day, all day. If you're using it that little, and it's working fine, I would say hold off on the service contract. Just keep it clean and if it stops working, turn it off and let it defrost for a few days. Usually that fixes everything because it's just iced over somewhere. Leica service contracts are a complete waste of money--only get them if you need to spend money (which was the case for us a few years ago, unfortunately, it's not now!). Also, don't get Leica to fix your cryostat! They charge twice as much as anyone else. One thing to remember to save your object cooling compressor motor, when you restart your cryostat after defrosting, cool the chamber first, then specimen head. That way, the specimen head (object temperature) compressor will not have to work as hard to keep itself cool while the chamber is cooling. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 8, 2011 at 3:11 PM, Amanda Madden wrote: > Hello Histonetters!!! > > I am writing today to ask about the necessity of having cryostats serviced > regularly. We have a Leica CM 3050S that is about 2 years old now. I have > been looking far and wide for recommendations about cryostat maintenance, > but haven't found much information. Do you think it is necessary to > purchase > a service contract that includes having someone come out regularly to check > on the equipment? We love our Leica rep, but I haven't wanted to contact > him > about this until I have a better idea of what other labs are doing. I > should > mention that ours is a very small lab, with sectioning time averaging > only about 5 hours per week. > > Thanks for any advice! > Amanda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brett_connolly <@t> merck.com Fri Apr 8 14:47:37 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Apr 8 14:47:41 2011 Subject: [Histonet] Leica Cryostat Service Recommendations In-Reply-To: References: Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D7BA0E5A@usctmx1176.merck.com> I'll second that, we have 4 of these (all > 6 yrs. old) and are very happy with them. We do not have service contracts and, when needed, have a local company come in for repairs and PMs. Good tip Emily about the object cooling compressor, thanks! Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, April 08, 2011 3:24 PM To: Amanda Madden; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Leica Cryostat Service Recommendations We have the same cryostat, three years old and this is the first time anything has broken on it. The total cost for everything will be about $600 which is waaaay cheaper than a $4000 service contract (for one year!). Also we use our cryostat almost every day, all day. If you're using it that little, and it's working fine, I would say hold off on the service contract. Just keep it clean and if it stops working, turn it off and let it defrost for a few days. Usually that fixes everything because it's just iced over somewhere. Leica service contracts are a complete waste of money--only get them if you need to spend money (which was the case for us a few years ago, unfortunately, it's not now!). Also, don't get Leica to fix your cryostat! They charge twice as much as anyone else. One thing to remember to save your object cooling compressor motor, when you restart your cryostat after defrosting, cool the chamber first, then specimen head. That way, the specimen head (object temperature) compressor will not have to work as hard to keep itself cool while the chamber is cooling. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 8, 2011 at 3:11 PM, Amanda Madden wrote: > Hello Histonetters!!! > > I am writing today to ask about the necessity of having cryostats serviced > regularly. We have a Leica CM 3050S that is about 2 years old now. I have > been looking far and wide for recommendations about cryostat maintenance, > but haven't found much information. Do you think it is necessary to > purchase > a service contract that includes having someone come out regularly to check > on the equipment? We love our Leica rep, but I haven't wanted to contact > him > about this until I have a better idea of what other labs are doing. I > should > mention that ours is a very small lab, with sectioning time averaging > only about 5 hours per week. > > Thanks for any advice! > Amanda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From jaylundgren <@t> gmail.com Fri Apr 8 14:56:54 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Apr 8 14:56:57 2011 Subject: [Histonet] Movat's Pentachrome Question In-Reply-To: <7267A64D75F58241B577876D8A885631038FE512@msgebe41> References: <7267A64D75F58241B577876D8A885631038FE512@msgebe41> Message-ID: Ummm...yes, the protocol for a Movat's Pentachrome is a mile long. It's got five different stains There is no one step Movat's Pentachrome. As a matter of fact, to me, it's always been shorthand for a pain in the a**. Tech one: "Any special stains today? I need to leave early." Tech two: "Just a Movat's Pentachrome." Tech one: " &%*#@$& !!!" Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) From DKBoyd <@t> chs.net Fri Apr 8 14:57:04 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Apr 8 14:57:15 2011 Subject: [Histonet] Breast fixation over the weekend Message-ID: What percentage of alcohol are you using to hold breast over the weekend, so as not to interfere with Her2 breast markers? Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From amkmadden <@t> gmail.com Fri Apr 8 17:53:37 2011 From: amkmadden <@t> gmail.com (Amanda Madden) Date: Fri Apr 8 17:54:02 2011 Subject: [Histonet] Leica Cryostat Service Recommendations In-Reply-To: <9FE33752FA3F3647BC85BCDC3EA6C3D7BA0E5A@usctmx1176.merck.com> References: <9FE33752FA3F3647BC85BCDC3EA6C3D7BA0E5A@usctmx1176.merck.com> Message-ID: Thank you everyone for, as usual, all of the fantastic advice! Glad to know that we can save some money and still know that we're taking good care of our favorite tool!! Amanda On Fri, Apr 8, 2011 at 3:47 PM, Connolly, Brett M wrote: > I'll second that, we have 4 of these (all > 6 yrs. old) and are very > happy with them. > We do not have service contracts and, when needed, have a local company > come in for repairs and PMs. > > Good tip Emily about the object cooling compressor, thanks! > > Brett M. Connolly, Ph.D. > Molecular Imaging Team Leader > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily > Sours > Sent: Friday, April 08, 2011 3:24 PM > To: Amanda Madden; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Leica Cryostat Service Recommendations > > We have the same cryostat, three years old and this is the first time > anything has broken on it. The total cost for everything will be about > $600 > which is waaaay cheaper than a $4000 service contract (for one year!). > Also we use our cryostat almost every day, all day. If you're using it > that > little, and it's working fine, I would say hold off on the service > contract. Just keep it clean and if it stops working, turn it off and > let > it defrost for a few days. Usually that fixes everything because it's > just > iced over somewhere. > Leica service contracts are a complete waste of money--only get them if > you > need to spend money (which was the case for us a few years ago, > unfortunately, it's not now!). > Also, don't get Leica to fix your cryostat! They charge twice as much as > anyone else. > One thing to remember to save your object cooling compressor motor, when > you > restart your cryostat after defrosting, cool the chamber first, then > specimen head. That way, the specimen head (object temperature) > compressor > will not have to work as hard to keep itself cool while the chamber is > cooling. > > Emily > > A great book should leave you with many experiences, and slightly > exhausted. > You should live several lives while reading it. > -William Styron > > > > On Fri, Apr 8, 2011 at 3:11 PM, Amanda Madden > wrote: > > > Hello Histonetters!!! > > > > I am writing today to ask about the necessity of having cryostats > serviced > > regularly. We have a Leica CM 3050S that is about 2 years old now. I > have > > been looking far and wide for recommendations about cryostat > maintenance, > > but haven't found much information. Do you think it is necessary to > > purchase > > a service contract that includes having someone come out regularly to > check > > on the equipment? We love our Leica rep, but I haven't wanted to > contact > > him > > about this until I have a better idea of what other labs are doing. I > > should > > mention that ours is a very small lab, with sectioning time averaging > > only about 5 hours per week. > > > > Thanks for any advice! > > Amanda > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > From pruegg <@t> ihctech.net Sat Apr 9 10:20:58 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Apr 9 10:21:03 2011 Subject: [Histonet] Re: QC documentation In-Reply-To: References: Message-ID: I agree with you Samurai, no amount of paper work takes the place of checking stain and section quality under the microscope with both the slide preparer and pathologist. I own my own lab now and I try to look at every slide that goes out the door, of course sometimes that is not possible, but I stress to my assistants that they must QC before sending anything out and if I hear from a customer about a poorly cut or stained slide they get hell from me. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Friday, April 08, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: QC documentation Let me add the dyspeptic observation that, in the many pathology services I've worked in in the last 30 years - the more QC paperwork I have to do, the worse the slides. I've never seen it done, but I think the only effective QC program in histology would be daily review of selected slides by a pathologist and a senior histotechnologist, with documentation of what problems were observed and what got done about them. I'm sure neither Six Sigma nor LEAN (whatever they may be) would approve of this Edwards Deming approach. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Apr 9 10:22:45 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Apr 9 10:22:48 2011 Subject: [Histonet] Movat's Pentachrome Question In-Reply-To: References: <7267A64D75F58241B577876D8A885631038FE512@msgebe41> Message-ID: <9223539AEA94477B832FBB1EBF656153@prueggihctechlt> Yes pentachrome is a pain to do, but when done properly I challenge you to find a more beautiful and useful stain, it is definitely my favorite to look at. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Friday, April 08, 2011 1:57 PM To: Herrick, James L. (Jim) Cc: histonet Subject: Re: [Histonet] Movat's Pentachrome Question Ummm...yes, the protocol for a Movat's Pentachrome is a mile long. It's got five different stains There is no one step Movat's Pentachrome. As a matter of fact, to me, it's always been shorthand for a pain in the a**. Tech one: "Any special stains today? I need to leave early." Tech two: "Just a Movat's Pentachrome." Tech one: " &%*#@$& !!!" Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Apr 9 10:27:54 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Apr 9 10:27:58 2011 Subject: [Histonet] Movat's Pentachrome Question In-Reply-To: <7267A64D75F58241B577876D8A885631038FE512@msgebe41> References: <7267A64D75F58241B577876D8A885631038FE512@msgebe41> Message-ID: <81DD18508347462CBF2438AD284CF8E2@prueggihctechlt> Are you talking about GMA or MMA plastic? I have not tried to do pentachrome on GMA but I have a great TC for plastics which I have used for years for bone and cartilage development. Nothing beats a pentachrome for arteries though so it might be worth pursuing, if I get a chance I will look over the protocol for pentachrome and offer some suggestions to you for GMA (that is what I use) sections. Contact me directly to remind me please. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. (Jim) Sent: Friday, April 08, 2011 11:10 AM To: histonet Subject: [Histonet] Movat's Pentachrome Question Happy Friday everyone!! I am trying to find a good Modified Movat's pentachrome stain protocol for plastic embedded iliac arteries and also bone tissue (animal model). Everything I have been able to find so far, is for paraffin embedded tissue and a mile long. There may not be a fix for the length of the protocol but if anyone would happen to have a technique that works well with plastic, I would greatly appreciate it. Thank you much. Have a great weekend!! Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Apr 9 10:56:28 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Apr 9 10:56:32 2011 Subject: [Histonet] patient melanin pigment In-Reply-To: References: <529110.84027.qm@web120714.mail.ne1.yahoo.com> Message-ID: <825CE82854C8451CAE34A0856571E133@prueggihctechlt> You can remove melanin but I would think that would have an adverse effect on IHC for sure, as Linda suggests the best bet for IHC is to use ap/red or aec with hrp instead of DAB. Jim Burchette has a giemsa stain he does on skin which colors the melanin green instead of brown but I was not able to get it to work myself. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, April 06, 2011 12:46 PM To: Michele Carr; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] patient melanin pigment Are you talking about staining with H&E, special stains or immunos? If its to stain with IHC you can use a chromogen other than DAB for your detection kit. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Carr Sent: Wednesday, April 06, 2011 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] patient melanin pigment Hi, I was wondering if there were a way to remove a patients melanin pigment on a slide. Any helpful advice would be appreciated. Thanks, Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Apr 9 11:03:47 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Apr 9 11:03:50 2011 Subject: [Histonet] science for kids In-Reply-To: References: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> Message-ID: <8EBC708A099048C9B620EE419E8D52CE@prueggihctechlt> Years ago ASCP I believe gave out coloring books for histology, or was it NSH? I got a couple for my grandkids, they love it, my 7 year old granddaughter just pulled it out and was talking to me about it last week. I have some flyers on histology I could email to you as well, I pass these out when I talk to kids about careers in histology. Please contact me directly at work and I will email them to you, I scanned them into my computer so it will be easy to send them to you. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret Blount Sent: Wednesday, April 06, 2011 1:55 AM To: Pam Barker; Emily Sours Cc: Histonet Subject: RE: [Histonet] science for kids Me too, even in the UK! Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: 05 April 2011 20:25 To: 'Emily Sours' Cc: 'Histonet' Subject: RE: [Histonet] science for kids Me TOO!!!! How do we get them? Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, April 05, 2011 3:19 PM To: Grantham, Andrea L - (algranth); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] science for kids A coloring book?! I want that for myself!!! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 5, 2011 at 1:49 PM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > Emily, > > NSH has a coloring book that explains histology. > > > Andi Grantham > > > > On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > > > I'm going to visit my nieces this summer and they want to know what > > I do > at > > work. Does anyone know of a good book or website that explains DNA > and/or > > histology for 4 to 6 year olds? Just thought I'd throw it out there, > since > > googling it would yield way more than I can deal with. > > > > Emily > > > > A great book should leave you with many experiences, and slightly > exhausted. > > You should live several lives while reading it. > > -William Styron > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Elliott <@t> hli.ubc.ca Sat Apr 9 15:38:30 2011 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Sat Apr 9 15:38:39 2011 Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 9 Message-ID: <4DA06156020000D600060E6D@mail.mrl.ubc.ca> We are needing to stain cardiac muscle for beta-actin. Anyone know of an antibody? most of the beta actin antibodies we have found say they do not stain cardiac muscle. Any suggestions greatly appreciated. Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From dan.kim.lockert <@t> sasktel.net Sun Apr 10 11:10:54 2011 From: dan.kim.lockert <@t> sasktel.net (Kim Lockert) Date: Sun Apr 10 11:11:14 2011 Subject: [Histonet] Fouchet's Message-ID: <6E5206DF-BC5E-4349-B6A6-DB97B5D3E41C@sasktel.net> Hi Does anyone use a counterstain other than Van Gieson's for the Fouchet's for Bile stain? We are trying to eliminate using picric acid in our lab. Thanks Kim From ccrowder <@t> vetmed.lsu.edu Sun Apr 10 22:14:30 2011 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Sun Apr 10 22:18:56 2011 Subject: [Histonet] Bile counterstain Message-ID: Kim - You can use eosin lightly to counterstain the bile stain. The green will still contrast well. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 From JMacDonald <@t> mtsac.edu Sun Apr 10 22:33:54 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Apr 10 22:34:01 2011 Subject: [Histonet] Bile counterstain In-Reply-To: Message-ID: or Nuclear Fast Red "Cheryl Crowder" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/10/2011 08:21 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Bile counterstain Kim - You can use eosin lightly to counterstain the bile stain. The green will still contrast well. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GaleL <@t> unionhospital.org Mon Apr 11 07:25:15 2011 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Mon Apr 11 07:25:23 2011 Subject: [Histonet] embedding forceps Message-ID: Good Morning! Can anyone recommend comfortable, not-heated, embedding forceps (with ordering info)? Our favorite that we had for years disappeared a while back and we haven't found a replacement that we really like yet......... Thank you, Gale Gale Limron CT, HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From jqb7 <@t> cdc.gov Mon Apr 11 07:31:33 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Mon Apr 11 07:31:38 2011 Subject: [Histonet] RE: embedding forceps In-Reply-To: References: Message-ID: I like these: You can get them in different lengths.....5 1/2" or 7" http://www.leica-microsystems.com/products/total-histology/consumables/autopsy-dissection/dissection/details/product/512-14cm-ergonomic-1/ Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Monday, April 11, 2011 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding forceps Good Morning! Can anyone recommend comfortable, not-heated, embedding forceps (with ordering info)? Our favorite that we had for years disappeared a while back and we haven't found a replacement that we really like yet......... Thank you, Gale Gale Limron CT, HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Mon Apr 11 09:24:47 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Apr 11 09:24:51 2011 Subject: [Histonet] science for kids In-Reply-To: <8EBC708A099048C9B620EE419E8D52CE@prueggihctechlt> References: <463F1CD3-B39B-445F-8C46-2410333B27A7@email.arizona.edu> <8EBC708A099048C9B620EE419E8D52CE@prueggihctechlt> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5651@PHSXMB30.partners.org> Patsy, I would appreciate receiving the flyers as well. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Saturday, April 09, 2011 12:04 PM To: 'Margaret Blount'; 'Pam Barker'; 'Emily Sours' Cc: 'Histonet' Subject: RE: [Histonet] science for kids Years ago ASCP I believe gave out coloring books for histology, or was it NSH? I got a couple for my grandkids, they love it, my 7 year old granddaughter just pulled it out and was talking to me about it last week. I have some flyers on histology I could email to you as well, I pass these out when I talk to kids about careers in histology. Please contact me directly at work and I will email them to you, I scanned them into my computer so it will be easy to send them to you. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret Blount Sent: Wednesday, April 06, 2011 1:55 AM To: Pam Barker; Emily Sours Cc: Histonet Subject: RE: [Histonet] science for kids Me too, even in the UK! Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: 05 April 2011 20:25 To: 'Emily Sours' Cc: 'Histonet' Subject: RE: [Histonet] science for kids Me TOO!!!! How do we get them? Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, April 05, 2011 3:19 PM To: Grantham, Andrea L - (algranth); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] science for kids A coloring book?! I want that for myself!!! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 5, 2011 at 1:49 PM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > Emily, > > NSH has a coloring book that explains histology. > > > Andi Grantham > > > > On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > > > I'm going to visit my nieces this summer and they want to know what > > I do > at > > work. Does anyone know of a good book or website that explains DNA > and/or > > histology for 4 to 6 year olds? Just thought I'd throw it out there, > since > > googling it would yield way more than I can deal with. > > > > Emily > > > > A great book should leave you with many experiences, and slightly > exhausted. > > You should live several lives while reading it. > > -William Styron > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Valerie.Hannen <@t> parrishmed.com Mon Apr 11 09:25:59 2011 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Apr 11 09:29:25 2011 Subject: [Histonet] Immuno's Message-ID: <5680DA93771F0C48954CC8D38425E72401AB351F@ISMAIL.parrishmed.local> Hi Folks.. Looking for a bit of help. Is there anyone out there who are doing their immuno's manually? Our Pathologist has just informed us that our immuno's are ALL too weak. He states that they always have been, as he compares them to slides that we get back from the reference labs that we use for the antibodies that we don't do in-house. If you are doing them manually...what companies are you using for antibodies, detection kit and chromogen? And are your stains really dark? I informed our Pathologist that I know for a fact that the reference labs that we use, do the immuno's on stainers, and naturally these stains will be consistently be stronger. I am trying to see if I can keep costs down by trying not to have to buy a stainer and continue to do the immuno's manually. If you all know of a wet-workshop or of a immuno. company who would send someone to our facility to teach us ( the latter is preferred, since my section chief is going to retire in 1 1/2 weeks, I will be taking over her duties and this is going to leave us with only myself and one other tech, until we can get a replacement for my position). Any help or advice that can be given will be greatly appreciated. Thanks so much. Valerie Hannen, MLT(ASCP),HTL,SU (FL) Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From trathborne <@t> somerset-healthcare.com Mon Apr 11 10:02:36 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Apr 11 10:03:41 2011 Subject: [Histonet] science for kids In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5651@PHSXMB30.partners.org> Message-ID: So would I! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sherwood, Margaret Sent: Monday, April 11, 2011 10:25 AM To: Patsy Ruegg; Margaret Blount; Pam Barker; Emily Sours Cc: Histonet Subject: RE: [Histonet] science for kids Patsy, I would appreciate receiving the flyers as well. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Saturday, April 09, 2011 12:04 PM To: 'Margaret Blount'; 'Pam Barker'; 'Emily Sours' Cc: 'Histonet' Subject: RE: [Histonet] science for kids Years ago ASCP I believe gave out coloring books for histology, or was it NSH? I got a couple for my grandkids, they love it, my 7 year old granddaughter just pulled it out and was talking to me about it last week. I have some flyers on histology I could email to you as well, I pass these out when I talk to kids about careers in histology. Please contact me directly at work and I will email them to you, I scanned them into my computer so it will be easy to send them to you. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret Blount Sent: Wednesday, April 06, 2011 1:55 AM To: Pam Barker; Emily Sours Cc: Histonet Subject: RE: [Histonet] science for kids Me too, even in the UK! Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: 05 April 2011 20:25 To: 'Emily Sours' Cc: 'Histonet' Subject: RE: [Histonet] science for kids Me TOO!!!! How do we get them? Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, April 05, 2011 3:19 PM To: Grantham, Andrea L - (algranth); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] science for kids A coloring book?! I want that for myself!!! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 5, 2011 at 1:49 PM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > Emily, > > NSH has a coloring book that explains histology. > > > Andi Grantham > > > > On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > > > I'm going to visit my nieces this summer and they want to know what > > I do > at > > work. Does anyone know of a good book or website that explains DNA > and/or > > histology for 4 to 6 year olds? Just thought I'd throw it out there, > since > > googling it would yield way more than I can deal with. > > > > Emily > > > > A great book should leave you with many experiences, and slightly > exhausted. > > You should live several lives while reading it. > > -William Styron > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From HornHV <@t> archildrens.org Mon Apr 11 10:06:08 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Apr 11 10:06:15 2011 Subject: [Histonet] RE: embedding forceps In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719484D42AF@EVS1.archildrens.org> These are the same ones we like. We like the 7" ones. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Monday, April 11, 2011 7:32 AM To: Gale Limron; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: embedding forceps I like these: You can get them in different lengths.....5 1/2" or 7" http://www.leica-microsystems.com/products/total-histology/consumables/autopsy-dissection/dissection/details/product/512-14cm-ergonomic-1/ Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Monday, April 11, 2011 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding forceps Good Morning! Can anyone recommend comfortable, not-heated, embedding forceps (with ordering info)? Our favorite that we had for years disappeared a while back and we haven't found a replacement that we really like yet......... Thank you, Gale Gale Limron CT, HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From trathborne <@t> somerset-healthcare.com Mon Apr 11 10:32:46 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Apr 11 10:33:54 2011 Subject: [Histonet] RE: embedding forceps In-Reply-To: <25A4DE08332B19499904459F00AAACB719484D42AF@EVS1.archildrens.org> Message-ID: We also use Mopec. They come in a few sizes, and our rep has worked with us to find something that I was having a hard time locating. http://www.mopec.com/category/947/ergonomic/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Horn, Hazel V Sent: Monday, April 11, 2011 11:06 AM To: 'Bartlett, Jeanine (CDC/OID/NCEZID)'; Gale Limron; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: embedding forceps These are the same ones we like. We like the 7" ones. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Monday, April 11, 2011 7:32 AM To: Gale Limron; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: embedding forceps I like these: You can get them in different lengths.....5 1/2" or 7" http://www.leica-microsystems.com/products/total-histology/consumables/autopsy-dissection/dissection/details/product/512-14cm-ergonomic-1/ Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Monday, April 11, 2011 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding forceps Good Morning! Can anyone recommend comfortable, not-heated, embedding forceps (with ordering info)? Our favorite that we had for years disappeared a while back and we haven't found a replacement that we really like yet......... Thank you, Gale Gale Limron CT, HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From kaylee <@t> medwrench.com Mon Apr 11 10:36:18 2011 From: kaylee <@t> medwrench.com (Kaylee McCaffrey) Date: Mon Apr 11 10:36:25 2011 Subject: [Histonet] Unsubscribe Message-ID: <71514EC6-A13D-4811-92DC-2C10DD7B3F7D@medwrench.com> Unsubscribe. Kaylee McCaffrey MedWrench Product Manager From trathborne <@t> somerset-healthcare.com Mon Apr 11 10:38:08 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Apr 11 10:38:55 2011 Subject: [Histonet] Immuno's In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB351F@ISMAIL.parrishmed.local> Message-ID: Who are you purchasing your antibodies from? Any of the companies that I have dealt with would gladly send someone out to optimize their antibodies. The fact that you are performing them manually probably is not the cause for the weak staining. We used to use the old Shandon Sequenza staining system as a backup if our Dako was out of service. When used with the same protocols, the staining was the same. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hannen, Valerie Sent: Monday, April 11, 2011 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno's Hi Folks.. Looking for a bit of help. Is there anyone out there who are doing their immuno's manually? Our Pathologist has just informed us that our immuno's are ALL too weak. He states that they always have been, as he compares them to slides that we get back from the reference labs that we use for the antibodies that we don't do in-house. If you are doing them manually...what companies are you using for antibodies, detection kit and chromogen? And are your stains really dark? I informed our Pathologist that I know for a fact that the reference labs that we use, do the immuno's on stainers, and naturally these stains will be consistently be stronger. I am trying to see if I can keep costs down by trying not to have to buy a stainer and continue to do the immuno's manually. If you all know of a wet-workshop or of a immuno. company who would send someone to our facility to teach us ( the latter is preferred, since my section chief is going to retire in 1 1/2 weeks, I will be taking over her duties and this is going to leave us with only myself and one other tech, until we can get a replacement for my position). Any help or advice that can be given will be greatly appreciated. Thanks so much. Valerie Hannen, MLT(ASCP),HTL,SU (FL) Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From chapcl <@t> yahoo.com Mon Apr 11 10:46:11 2011 From: chapcl <@t> yahoo.com (William Chappell) Date: Mon Apr 11 10:46:15 2011 Subject: [Histonet] Immuno's In-Reply-To: Message-ID: <533867.64897.qm@web30601.mail.mud.yahoo.com> Toni beat me to it. My suggestion is to contact the vendor where you purchase your detection. The technical support departments for immuno vendors are emmensely helpful. Try Dako at 800-400-DAKO, Biocare at 603-925-8000, or Cellmarque at 800.665.7284. In addition, it has always been my experience that a good hand stain is always better than an instrument stain -- primarily because they are better washed by hand. My best guess is that you are not draining your slides well enough between rinses. What I do is flood my slides with buffer, then put them at an angle (the Biocare IQ stainer is great for this) and rinse again -- Robin Simpkins at Biocare calls this the Jamaican waterfall. I then follow up with a flick of the slide, then proceed to the next step. Again call your vendors. Technical support will be extremely helpful and you may get an expert (applications specialist or TSR) out to help you in person. William Chappell, HTL(ASCP)QIHC IHC Development Specialist CellNetix Pathology & Laboratories (503) 358-9567 www.cellnetix.com "Our expertise...your peace of mind" --- On Mon, 4/11/11, Rathborne, Toni wrote: > From: Rathborne, Toni > Subject: RE: [Histonet] Immuno's > To: "Hannen, Valerie" , histonet@lists.utsouthwestern.edu > Date: Monday, April 11, 2011, 8:38 AM > Who are you purchasing your > antibodies from? Any of the companies that I have dealt with > would gladly send someone out to optimize their antibodies. > The fact that you are performing them manually probably is > not the cause for the weak staining. We used to use the old > Shandon Sequenza staining system as a backup if our Dako was > out of service. When used with the same protocols, the > staining was the same. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On > Behalf Of Hannen, > Valerie > Sent: Monday, April 11, 2011 10:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Immuno's > > > Hi Folks.. > > Looking for a bit of help. Is there anyone out there who > are doing their > immuno's manually?? Our Pathologist has just informed > us that our > immuno's are ALL too weak. > He states that they always have been, as he compares them > to slides that > we get back from the? reference labs that we use for > the antibodies that > we don't do in-house. > > If you are doing them manually...what companies are you > using for > antibodies, detection kit and chromogen? And are your > stains really > dark? > > I informed our Pathologist that I know for a fact that the > reference > labs that we use, do the immuno's on stainers, and > naturally these > stains will be consistently be stronger. > > I am trying to see if I can keep costs down by trying not > to have to buy > a stainer and continue to do the immuno's manually. > > If you all know of a wet-workshop or of a immuno. company > who would send > someone to our facility to teach us ( the latter is > preferred, since my > section chief is going to retire in 1 1/2 weeks, I will be > taking over > her duties and this is going to leave us with only myself > and one other > tech, until we can get a replacement for my position). > > Any help or advice that can be given will be greatly > appreciated. > > Thanks so much. > > Valerie Hannen, MLT(ASCP),HTL,SU (FL) > Parrish Medical Center > Titusville,Florida > > > ************************************************************** > "This email is intended solely for the use of the > individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from > disclosure > under applicable law. If the reader of this email is not > the > intended recipient or the employee or agent responsible > for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If > you > have received this communication in error, please > immediately > delete this message. Thank you" > ************************************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset > Medical Center > and are intended only for the addressee.? The > information contained in this > message is confidential and may contain privileged, > confidential, > proprietary and/or trade secret information entitled to > protection and/or > exemption from disclosure under applicable law.? > Unauthorized forwarding, > printing, copying, distribution, or use of such information > is strictly > prohibited and may be unlawful.? If you are not the > addressee, please > promptly delete this message and notify the sender of the > delivery error > by e-mail or you may call Somerset Medical Center's > computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date > news, > event listings, health information and more. > > -----Inline Attachment Follows----- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Mon Apr 11 10:48:41 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Apr 11 10:48:48 2011 Subject: [Histonet] Benchmark Ultra question Message-ID: <3F8B4FBC9BAF43628DFAE0F32E7C3BDA@dielangs.at> Hi! I have some issues with our new Ultra. Since a few days antibodies, that work usually well, show up very faint. For example the TTF1 (Novocastra). I ordered a new bottle, made a titration and found, that the "old" titer 1:50 was again well enough. I filled the old PrepKit - and the result was very weak. Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - and again the result was very weak. At that time the working-solution was only three days old. Has anyone an explanation, why the titration works well and the automated dispension doesn't? Regards Gudrun Lang From alyssa <@t> alliedsearchpartners.com Mon Apr 11 10:55:57 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa) Date: Mon Apr 11 10:56:04 2011 Subject: [Histonet] Histology Manager Needed in NY Message-ID: Hello Everyone, I am looking for a Night Shift Histology Manager in New York. The position is in the White Plains area. Message me for details! Thank you! Alyssa Peterson, Candidate Recruitment LinkedIn: http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From cpyse <@t> x-celllab.com Mon Apr 11 11:37:11 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Mon Apr 11 11:38:02 2011 Subject: [Histonet] Immuno's In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB351F@ISMAIL.parrishmed.local> References: <5680DA93771F0C48954CC8D38425E72401AB351F@ISMAIL.parrishmed.local> Message-ID: <001b01cbf866$b50ad610$1f208230$@com> Do you use a hydrophobic pen to circle the tissue. This concentrates the solution where you need it. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 etx.232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, April 11, 2011 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno's Hi Folks.. Looking for a bit of help. Is there anyone out there who are doing their immuno's manually? Our Pathologist has just informed us that our immuno's are ALL too weak. He states that they always have been, as he compares them to slides that we get back from the reference labs that we use for the antibodies that we don't do in-house. If you are doing them manually...what companies are you using for antibodies, detection kit and chromogen? And are your stains really dark? I informed our Pathologist that I know for a fact that the reference labs that we use, do the immuno's on stainers, and naturally these stains will be consistently be stronger. I am trying to see if I can keep costs down by trying not to have to buy a stainer and continue to do the immuno's manually. If you all know of a wet-workshop or of a immuno. company who would send someone to our facility to teach us ( the latter is preferred, since my section chief is going to retire in 1 1/2 weeks, I will be taking over her duties and this is going to leave us with only myself and one other tech, until we can get a replacement for my position). Any help or advice that can be given will be greatly appreciated. Thanks so much. Valerie Hannen, MLT(ASCP),HTL,SU (FL) Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Apr 11 11:48:04 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Apr 11 11:48:14 2011 Subject: AW: [Histonet] Benchmark Ultra question In-Reply-To: <4DA2EE9A.2B7F.00C9.1@geisinger.edu> References: <3F8B4FBC9BAF43628DFAE0F32E7C3BDA@dielangs.at> <4DA2EE9A.2B7F.00C9.1@geisinger.edu> Message-ID: <881AD8F57F7B4ECF84F1411A606A68C4@dielangs.at> Yes, that could be true. Some of the PrepKits "go harder" than others. Perhaps the pressure of the hammer is not big enough. Sometimes the nozzles are not filled equally. (We press always reagens in the nozzle when we prepare the run.) With the Ventana-System there is an additional dilution, because the working-solution is added to a reaction-buffer film under the LCS. Perhaps it makes a difference if you dispense the solution with the pipett-tip under the LCS or if the drop falls on the surface of the LCS. That could be a matter of the LCS-quality, or buffer-quantity , or . I think I'll become mad! Our pathologists have an uncertain unhappiness with the overall staining results, but most of the antibodies work well. Perhaps the majority isn't very sensitiv to small changes in the system and the quality-difference isn't big enough, but some are easier to "kill". Regards, Gudrun _____ Von: Angela Bitting [mailto:akbitting@geisinger.edu] Gesendet: Montag, 11. April 2011 18:06 An: gu.lang@gmx.at Betreff: Re: [Histonet] Benchmark Ultra question When I was trained to do titration runs on the BenchmarkXT and Ultras, I was told to titrate 100ul of antibody. I have been suspecting for some time now that the dispensers DON'T dispense a full 100ul. I haven't taken the time to prove it, but your question may have motivated me. That could explain the weak staining. >>> "Gudrun Lang" 4/11/2011 11:48 AM >>> Hi! I have some issues with our new Ultra. Since a few days antibodies, that work usually well, show up very faint. For example the TTF1 (Novocastra). I ordered a new bottle, made a titration and found, that the "old" titer 1:50 was again well enough. I filled the old PrepKit - and the result was very weak. Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - and again the result was very weak. At that time the working-solution was only three days old. Has anyone an explanation, why the titration works well and the automated dispension doesn't? Regards Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From eugenia902d1 <@t> hotmail.com Mon Apr 11 12:06:59 2011 From: eugenia902d1 <@t> hotmail.com (Eugenia Thomas) Date: Mon Apr 11 12:07:03 2011 Subject: [Histonet] 1 H&E slide vs. 2 Message-ID: Good afternoon everyone, Does anyone know of an article or statistics discussing the impact (medical diagnosing) of cutting 2 H&E slides per block verses 1 for all routine work? Genia From Gary_Steinke <@t> vwr.com Mon Apr 11 13:02:54 2011 From: Gary_Steinke <@t> vwr.com (Gary_Steinke@vwr.com) Date: Mon Apr 11 13:03:05 2011 Subject: [Histonet] Gary Steinke is out of the office Message-ID: I will be out of the office starting 04/11/2011 and will not return until 04/18/2011. I will be out of the office from April 11th and returning on April 18th. I will out of town attending a business meeting and will have very limited access to email. If you need assistance immediately, please contact Customer Service at 800-932-5000. Take care and have a great day. From Ken_Marissael <@t> vwr.com Mon Apr 11 13:03:50 2011 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Mon Apr 11 13:03:58 2011 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 04/11/2011 and will not return until 04/15/2011. I will be unavailable and will have limited access to phone and e-mail. While I am away, please contact VWR Customer Service at 800-932-5000. From TGoins <@t> mt.gov Mon Apr 11 13:47:45 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Apr 11 13:47:49 2011 Subject: [Histonet] RE: Immuno's In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB351F@ISMAIL.parrishmed.local> References: <5680DA93771F0C48954CC8D38425E72401AB351F@ISMAIL.parrishmed.local> Message-ID: Valerie - All of our IHC stains are done manually. As an animal tissue lab, our antibody sources will differ, but the best chromagen I have use by far is Biocare's Vulcan Fast Red. I have tried other sources (cheaper) for the new fuchsin chromagen, but they do not produce the same vivid red stain as Vulcan. Our secondary antibodies are from KPL (biotinylated anti-mouse or anti-rabbit) with KPL alkaline phosphatase. Good luck Tresa Goins Histopathology Supervisor Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, April 11, 2011 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno's Hi Folks.. Looking for a bit of help. Is there anyone out there who are doing their immuno's manually? Our Pathologist has just informed us that our immuno's are ALL too weak. He states that they always have been, as he compares them to slides that we get back from the reference labs that we use for the antibodies that we don't do in-house. If you are doing them manually...what companies are you using for antibodies, detection kit and chromogen? And are your stains really dark? I informed our Pathologist that I know for a fact that the reference labs that we use, do the immuno's on stainers, and naturally these stains will be consistently be stronger. I am trying to see if I can keep costs down by trying not to have to buy a stainer and continue to do the immuno's manually. If you all know of a wet-workshop or of a immuno. company who would send someone to our facility to teach us ( the latter is preferred, since my section chief is going to retire in 1 1/2 weeks, I will be taking over her duties and this is going to leave us with only myself and one other tech, until we can get a replacement for my position). Any help or advice that can be given will be greatly appreciated. Thanks so much. Valerie Hannen, MLT(ASCP),HTL,SU (FL) Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathderm <@t> yahoo.com Mon Apr 11 14:02:46 2011 From: pathderm <@t> yahoo.com (robert king) Date: Mon Apr 11 14:02:49 2011 Subject: [Histonet] Re:2 Message-ID: <80862.74774.qm@web111706.mail.gq1.yahoo.com> ...I hope you?ll enjoy after visiting this site. http://kristiang.kr.funpic.de/page.php?mafortune=65m5 From histonet.nospam <@t> vneubert.com Mon Apr 11 14:32:34 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Mon Apr 11 14:32:43 2011 Subject: [Histonet] Re:2 In-Reply-To: <80862.74774.qm@web111706.mail.gq1.yahoo.com> References: <80862.74774.qm@web111706.mail.gq1.yahoo.com> Message-ID: <4DA35752.40204@vneubert.com> Redirects to a site which offers pills (I cannot even see what pills...) > ...I hope you?ll enjoy after visiting this site. http://kristiang.kr.funpic.de/page.php?mafortune=65m5 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lchen <@t> mednet.ucla.edu Mon Apr 11 15:56:40 2011 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Mon Apr 11 15:57:02 2011 Subject: [Histonet] Used Laboratory Equipment Message-ID: Hi, Does anyone which are better websites to place ads to sell laboratory equipment that are reliable and well-trafficked? We are looking to sell a Tecan machine. Thank you! Leslie From rboen <@t> clearwire.net Mon Apr 11 17:03:49 2011 From: rboen <@t> clearwire.net (Rick and Sue Boen) Date: Mon Apr 11 17:03:58 2011 Subject: [Histonet] P16 and ProExC Substitutions Message-ID: <1A2DEE32D7BA4FA8A99F8541356DA889@OfficeComputer> Hi All, Now that MTM has made P16 very expensive and TriPath is have problems producing ProExC, what's everybody using on ffpe tissue? We were told by TriPath/BD that ProExC will probably not be available until late June. Rick Boen, BS, HTL(ASCP) St. Luke's Hospital 901 East 1st Street Duluth, Mn 55805 From Pitts.Jaclyn <@t> mayo.edu Mon Apr 11 20:32:04 2011 From: Pitts.Jaclyn <@t> mayo.edu (Pitts, Jaclyn S. (Jackie), HT(ASCP)) Date: Mon Apr 11 20:32:09 2011 Subject: [Histonet] NY cert requirements Message-ID: Hello, My colleague and I have been wondering what the certification requirements are in New York and what other kind of guidelines that have to be followed for the state as well in the lab. Jaclyn Pitts, HT(ASCP) Histotechnician E-mail: pitts.jaclyn@mayo.edu From kmrstanley <@t> gmail.com Mon Apr 11 21:13:17 2011 From: kmrstanley <@t> gmail.com (Kim Stanley) Date: Mon Apr 11 21:13:21 2011 Subject: [Histonet] REMOVE FROM LIST Message-ID: Please remove this email address from this list. Thank you, From jane.weber <@t> comcast.net Tue Apr 12 00:16:48 2011 From: jane.weber <@t> comcast.net (Jane Weber) Date: Tue Apr 12 00:16:51 2011 Subject: [Histonet] embedding forceps Message-ID: There is a company that makes a great tool for embedding tissue. I have used it and love it. Their website is Histopress.com Good luck! From jane.weber <@t> comcast.net Tue Apr 12 00:23:48 2011 From: jane.weber <@t> comcast.net (Jane Weber) Date: Tue Apr 12 00:23:46 2011 Subject: [Histonet] RE: embedding forceps Message-ID: <84014864CADB4AC087F755A1B4FE5AFE@janesnotebook> There is also a company that makes great embedding presses called Histopress. I use them and love them! Check Histopress.com From Candy.A.Bales <@t> uth.tmc.edu Tue Apr 12 08:19:08 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Tue Apr 12 08:19:12 2011 Subject: [Histonet] Tissue-Tek cover slipper question Message-ID: <62C915811DD5A142851D95CA6BC5D1E41B371A7E0B@UTHCMS1.uthouston.edu> Good morning. I work part-time at a small lab and they have a Sakura Feintek Tissue-Tek SCA tape coverslipper, model 4764. I have not worked with any tape coverslippers before and am having problems with areas of the tissue drying out. The solvent bottle is full of Xylene. However, there is a crack in the lid of the bottle. After removing the stained slides from the autostainer and loading the basket(s) to place on the cover slipper, I re-dip the slides in Xylene. The room where this equipment is housed, does get warm. I was wondering if the tape can become old & brittle, leading to it not sealing well, or if not enough Xylene is being deposited on each slide or a combination of both? I could not find the manual on the unit for trouble shooting ideas. Any help would be greatly appreciated. Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From sadams <@t> hcmhcares.org Tue Apr 12 08:28:30 2011 From: sadams <@t> hcmhcares.org (Sharon Adams) Date: Tue Apr 12 08:28:49 2011 Subject: [Histonet] remove me from list Message-ID: Please remove this email from the list. sadams@hcmhcares.org -- *Sharon Adams* From LSetlak <@t> childrensmemorial.org Tue Apr 12 08:33:57 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Tue Apr 12 08:34:40 2011 Subject: [Histonet] RE: Tissue-Tek cover slipper question In-Reply-To: <62C915811DD5A142851D95CA6BC5D1E41B371A7E0B@UTHCMS1.uthouston.edu> References: <62C915811DD5A142851D95CA6BC5D1E41B371A7E0B@UTHCMS1.uthouston.edu> Message-ID: <7111DB39D045004C9CF29E79C71B28BC101D507911@CMHEXCC01MBX.childrensmemorial.org> Are you letting the tape sit out ("cure") for awhile befoe you use it? We leave ours out overnight? Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bales, Candy A Sent: Tuesday, April 12, 2011 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue-Tek cover slipper question Good morning. I work part-time at a small lab and they have a Sakura Feintek Tissue-Tek SCA tape coverslipper, model 4764. I have not worked with any tape coverslippers before and am having problems with areas of the tissue drying out. The solvent bottle is full of Xylene. However, there is a crack in the lid of the bottle. After removing the stained slides from the autostainer and loading the basket(s) to place on the cover slipper, I re-dip the slides in Xylene. The room where this equipment is housed, does get warm. I was wondering if the tape can become old & brittle, leading to it not sealing well, or if not enough Xylene is being deposited on each slide or a combination of both? I could not find the manual on the unit for trouble shooting ideas. Any help would be greatly appreciated. Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Kuhnla <@t> chsli.org Tue Apr 12 08:36:06 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Tue Apr 12 08:36:14 2011 Subject: [Histonet] Her2 FISH controls Message-ID: Hello, I see that the ASCO/CAP Guidelines recommend using amplified, cutoff, and normal controls for Her2 FISH testing. We currently use Pathvysion and purchase normal and cutoff controls from Abbott. They do not offer a product of amplified controls. Are you all running this third control? Are you using in-house tissue? Have you found a vendor who sells them? Thanx Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From Candy.A.Bales <@t> uth.tmc.edu Tue Apr 12 09:20:51 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Tue Apr 12 09:20:55 2011 Subject: [Histonet] Tiisue tek coverslipper Message-ID: <62C915811DD5A142851D95CA6BC5D1E41B371A7E33@UTHCMS1.uthouston.edu> Thank you all for your suggestions. I will observe the drip rate and increase it as well as replacing the cracked lid on the solvent bottle. I did leave the tape out for a couple of days before placing it on the machine as a friend who has a different model suggested I do so. Thank you again Histonetters candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From eca9 <@t> georgetown.edu Tue Apr 12 10:37:17 2011 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Tue Apr 12 10:37:25 2011 Subject: [Histonet] Counterstain for dual chromogenic Message-ID: <4DA471AD.3040002@georgetown.edu> Good morning, I have been attempting to do some dual chromogenic staining. I am using a mouse and a rabbit antibody. For the mouse antibody I am using an HRP-mouse secondary followed by AEC for visualization. For the rabbit antibody I am using a biotinylated rabbit secondary, followed by ABC-AP and vector blue. My problem is what to use as a counterstain. From everything I have read so far there isn't one that would work without having some problems. I was thinking of trying a very light Hematoxylin and see if it doesn't disrupt the vector blue visualization too much. Does anyone else have any suggestions? Thanks, Eva From Margaret.Perry <@t> sdstate.edu Tue Apr 12 11:08:13 2011 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Apr 12 11:08:19 2011 Subject: [Histonet] microwave processors Message-ID: Has anyone used the LabPulse microwave processor from EBS? We need a new microwave for staining and retrieval and thought maybe we could get one that will do processing also. I've looked at the Shurwave from Fisher, and the Mars from Hacker. Are there any other companies that have small microwave processors? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Apr 12 11:10:35 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Apr 12 11:11:11 2011 Subject: [Histonet] RE: NY cert requirements In-Reply-To: References: Message-ID: That is a huge question. Here is a good place to start NYS Clinical Laboratory TechnologySep 9, 2009 ... Clinical Laboratory Technician. License Requirements ... www.op.nysed.gov Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) [Pitts.Jaclyn@mayo.edu] Sent: Monday, April 11, 2011 9:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NY cert requirements Hello, My colleague and I have been wondering what the certification requirements are in New York and what other kind of guidelines that have to be followed for the state as well in the lab. Jaclyn Pitts, HT(ASCP) Histotechnician E-mail: pitts.jaclyn@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 12 11:14:43 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 12 11:14:47 2011 Subject: [Histonet] Counterstain for dual chromogenic In-Reply-To: <4DA471AD.3040002@georgetown.edu> Message-ID: <915944.67950.qm@web65705.mail.ac4.yahoo.com> You could do that or you could use DAB for one antibody ("brownish" color), followed by DAB + Nickel for the other ("bluish" color) and they are both permanent and alcohol resistant. Ren? J. --- On Tue, 4/12/11, Eva Permaul wrote: From: Eva Permaul Subject: [Histonet] Counterstain for dual chromogenic To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 12, 2011, 11:37 AM Good morning, I have been attempting to do some dual chromogenic staining. I am using a mouse and a rabbit antibody. For the mouse antibody I am using an HRP-mouse secondary followed by AEC for visualization. For the rabbit antibody I am using a biotinylated rabbit secondary, followed by ABC-AP and vector blue. My problem is what to use as a counterstain. From everything I have read so far there isn't one that would work without having some problems. I was thinking of trying a very light Hematoxylin and see if it doesn't disrupt the vector blue visualization too much. Does anyone else have any suggestions? Thanks, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 12 11:16:00 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 12 11:16:05 2011 Subject: [Histonet] microwave processors In-Reply-To: Message-ID: <566652.85502.qm@web65713.mail.ac4.yahoo.com> Milestone manufactures very good and versatile MW that process and stain. Ren? J. --- On Tue, 4/12/11, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] microwave processors To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, April 12, 2011, 12:08 PM Has anyone used the LabPulse microwave processor from EBS?? We need a new microwave for staining and retrieval? and thought maybe we could get one that will do processing also.???I've looked at the Shurwave from Fisher, and the Mars from Hacker.? Are there any other companies that have small microwave processors? Margaret Perry HT(ASCP) Dept of Veterinary and? Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Apr 12 11:18:43 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 12 11:18:55 2011 Subject: AW: [Histonet] Her2 FISH controls In-Reply-To: References: Message-ID: We use inhouse tissue and made really tiny pieces to place them near the specimen slide. So we bring it beneath one coverslip. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kuhnla, Melissa Gesendet: Dienstag, 12. April 2011 15:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Her2 FISH controls Hello, I see that the ASCO/CAP Guidelines recommend using amplified, cutoff, and normal controls for Her2 FISH testing. We currently use Pathvysion and purchase normal and cutoff controls from Abbott. They do not offer a product of amplified controls. Are you all running this third control? Are you using in-house tissue? Have you found a vendor who sells them? Thanx Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Apr 12 11:23:27 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 12 11:23:33 2011 Subject: [Histonet] Benchmark Ultra question - follow up In-Reply-To: <881AD8F57F7B4ECF84F1411A606A68C4@dielangs.at> References: <3F8B4FBC9BAF43628DFAE0F32E7C3BDA@dielangs.at><4DA2EE9A.2B7F.00C9.1@geisinger.edu> <881AD8F57F7B4ECF84F1411A606A68C4@dielangs.at> Message-ID: <846C2F83097F4C4C935C3BB8E7A6B215@dielangs.at> Today I made a further experiment. I did two titration runs. On one slide I added the working solution by pipette on the other slide I added it by pushing the filled PrepKit. Both stainings came out beautiful. - There has to be something wrong with the dispension-amount. Ventana is informed, I hope they find the culprit. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Montag, 11. April 2011 18:48 An: 'Angela Bitting' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] Benchmark Ultra question Yes, that could be true. Some of the PrepKits "go harder" than others. Perhaps the pressure of the hammer is not big enough. Sometimes the nozzles are not filled equally. (We press always reagens in the nozzle when we prepare the run.) With the Ventana-System there is an additional dilution, because the working-solution is added to a reaction-buffer film under the LCS. Perhaps it makes a difference if you dispense the solution with the pipett-tip under the LCS or if the drop falls on the surface of the LCS. That could be a matter of the LCS-quality, or buffer-quantity , or . I think I'll become mad! Our pathologists have an uncertain unhappiness with the overall staining results, but most of the antibodies work well. Perhaps the majority isn't very sensitiv to small changes in the system and the quality-difference isn't big enough, but some are easier to "kill". Regards, Gudrun _____ Von: Angela Bitting [mailto:akbitting@geisinger.edu] Gesendet: Montag, 11. April 2011 18:06 An: gu.lang@gmx.at Betreff: Re: [Histonet] Benchmark Ultra question When I was trained to do titration runs on the BenchmarkXT and Ultras, I was told to titrate 100ul of antibody. I have been suspecting for some time now that the dispensers DON'T dispense a full 100ul. I haven't taken the time to prove it, but your question may have motivated me. That could explain the weak staining. >>> "Gudrun Lang" 4/11/2011 11:48 AM >>> Hi! I have some issues with our new Ultra. Since a few days antibodies, that work usually well, show up very faint. For example the TTF1 (Novocastra). I ordered a new bottle, made a titration and found, that the "old" titer 1:50 was again well enough. I filled the old PrepKit - and the result was very weak. Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - and again the result was very weak. At that time the working-solution was only three days old. Has anyone an explanation, why the titration works well and the automated dispension doesn't? Regards Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nelsonrnch <@t> verizon.net Tue Apr 12 11:30:12 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Tue Apr 12 11:30:15 2011 Subject: [Histonet] Milestone MW Message-ID: <154600.6005.qm@web84304.mail.re1.yahoo.com> Margret, Which microwave would that be ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS 35-900 Bob Hope Drive Suite 275 Rancho Mirage, Ca.? 92270 cell (909) 841-9761 / wk (760) 321-2500 nelsonrnch@verizon.net From Diane <@t> bioview.co.il Tue Apr 12 12:23:03 2011 From: Diane <@t> bioview.co.il (Diane Kolins) Date: Tue Apr 12 12:16:17 2011 Subject: [Histonet] Immunofluorescence and FISH Combined stating Message-ID: Where can I find a procedure for dual staining of blood and bone marrow smears using Vector anti-kappa and anti-lambda tagged with Coumarine followed by FISH hybridization. I am able to see what I think are plasma cells by their fluorescent blue cytoplasm and on the same slide (changing filters) I can see the red and green signal patterns. From: : diane@bioview.co.il From Dorothy.L.Webb <@t> HealthPartners.Com Tue Apr 12 12:35:18 2011 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Apr 12 12:35:22 2011 Subject: [Histonet] Von kossa stain Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From sbreeden <@t> nmda.nmsu.edu Tue Apr 12 12:52:49 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Apr 12 12:52:52 2011 Subject: [Histonet] Von kossa stain In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E477BD@nmdamailsvr.nmda.ad.nmsu.edu> Funny you should ask... just last week I had two requests for a Von Kossa. That's when I found out that, despite the fact that we just moved into a brand new building with all the bells and whistles, there was not one single UV light in any of the many hoods that had been installed. Heaven forbid I could even find an incandescent bulb (we are a "green building"). So, I prepped my slides, put them in a clear glass Coplin jar and parked the jar on the hood of my car for an hour. Works like a charm. This all depends, naturally, on (1) if you even HAVE sunshine where you live; (2) how far a walk it is to the sunshine, and (3) whether you have a car hood on which to park the Coplin jar. Needless to say, out of the line of sight of curious onlookers with sticky fingers and no business wondering what that glass jar is... Nonetheless, it works just fine! May the Force be with you. From Mark.Elliott <@t> hli.ubc.ca Tue Apr 12 13:16:16 2011 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Tue Apr 12 13:16:55 2011 Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 12 In-Reply-To: <5A324AD4.955@mail.mrl.ubc.ca> References: <5A324AD4.955@mail.mrl.ubc.ca> Message-ID: <4DA43480020000D60006111F@mail.mrl.ubc.ca> Date: Tue, 12 Apr 2011 11:37:17 -0400 From: Eva Permaul Subject: [Histonet] Counterstain for dual chromogenic To: histonet@lists.utsouthwestern.edu Message-ID: <4DA471AD.3040002@georgetown.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Eva I have been doing some double labelling using Vulcan Fast Red from Biocare and Vector Blue Alkaline Phosphatase Substrate Kit III from Vector Labs. For counter stain I use Vector Methyl Green and the combination works great and looks quite nice. Mark Good morning, I have been attempting to do some dual chromogenic staining. I am using a mouse and a rabbit antibody. For the mouse antibody I am using an HRP-mouse secondary followed by AEC for visualization. For the rabbit antibody I am using a biotinylated rabbit secondary, followed by ABC-AP and vector blue. My problem is what to use as a counterstain. From everything I have read so far there isn't one that would work without having some problems. I was thinking of trying a very light Hematoxylin and see if it doesn't disrupt the vector blue visualization too much. Does anyone else have any suggestions? Thanks, Eva ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From gu.lang <@t> gmx.at Tue Apr 12 13:22:22 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 12 13:22:43 2011 Subject: AW: [Histonet] Von kossa stain In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> Message-ID: <13B705C625E74F1BB9337593D57C3658@dielangs.at> WE use just a simple desk-lamp and put the coplin jar directly under the bulb. And the desk-lamp is placed in the window. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, Dorothy L Gesendet: Dienstag, 12. April 2011 19:35 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Von kossa stain What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Tue Apr 12 13:35:47 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Apr 12 13:36:21 2011 Subject: AW: [Histonet] Von kossa stain In-Reply-To: <13B705C625E74F1BB9337593D57C3658@dielangs.at> References: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> <13B705C625E74F1BB9337593D57C3658@dielangs.at> Message-ID: <59222C2B-42C1-4EB6-8233-ED0FB7FCA354@email.arizona.edu> We have intense sun here but my windows face the wrong way! At least I have windows. I just use a light bulb from the lamp we have over the coverslipping station. Works great. Andi On Apr 12, 2011, at 11:22 AM, Gudrun Lang wrote: > WE use just a simple desk-lamp and put the coplin jar directly under the > bulb. And the desk-lamp is placed in the window. > > Bye Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, > Dorothy L > Gesendet: Dienstag, 12. April 2011 19:35 > An: 'histonet@lists.utsouthwestern.edu' > Betreff: [Histonet] Von kossa stain > > What is everyone using for their "light" when developing the silver in the > VonKossa stain when you have no sunlight to use? We used to use a 60 watt > lamp, but haven't done one for years and am bringing this stain back to our > repetiore due to pathologist request. Thanks much! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From polavarapu.mahesh <@t> gmail.com Tue Apr 12 13:56:53 2011 From: polavarapu.mahesh <@t> gmail.com (Mahesh Polavarapu) Date: Tue Apr 12 13:56:56 2011 Subject: [Histonet] Staining using a Plastic Sytem Message-ID: Hi, Does anyone have experience embedding and staining in plastic? We have rabbit rotator cuff tendons that are being embedded in plastic. I am trying to figure out what the process is and which stains to use in order to visualize (and quantify) blood vessels and collagen deposition at the Bone (Humeral Head) - Tendon (Infraspinatus) interface. Thanks, Mahesh From aevans3 <@t> lghealth.org Tue Apr 12 13:57:18 2011 From: aevans3 <@t> lghealth.org (Evans, Andria B) Date: Tue Apr 12 13:59:21 2011 Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor Message-ID: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60@MAIL-AG-CLUSTER.lha.org> Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is.... Formalin 1 min 37degrees Formalin 15 mins 37degrees 70 14 mins 40 degrees 95 14 mins 40 degrees 95 9 mins 40 degrees 100 9 mins 40 degrees 100 7 mins 40 degrees 100 4 mins 40 degrees Xylene 23 mins no heat Xylene 15 mins no heat Paraffin 20 mins 60 degrees Paraffin 18 mins 60 degrees Paraffin 10 mins 60 degrees Paraffin 0 mins 60 degrees All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges. Any feedback would be greatly appreciated. Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 aevans3@lgheath.org This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Tue Apr 12 14:27:49 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 12 14:27:53 2011 Subject: [Histonet] Milestone MW In-Reply-To: <154600.6005.qm@web84304.mail.re1.yahoo.com> Message-ID: <809117.78546.qm@web65715.mail.ac4.yahoo.com> Model "KOS" by Milestone is capable of tissue processing, decalcification, special stains, fixation, gross hardening, and antigen retrieval. Ren? J. --- On Tue, 4/12/11, SHANE NELSON wrote: From: SHANE NELSON Subject: [Histonet] Milestone MW To: Histonet@lists.utsouthwestern.edu Date: Tuesday, April 12, 2011, 12:30 PM Margret, Which microwave would that be ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS 35-900 Bob Hope Drive Suite 275 Rancho Mirage, Ca.? 92270 cell (909) 841-9761 / wk (760) 321-2500 nelsonrnch@verizon.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 12 14:30:26 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 12 14:30:30 2011 Subject: [Histonet] Von kossa stain In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> Message-ID: <145774.98333.qm@web65704.mail.ac4.yahoo.com> You can use any strong intensity microscope light. The stronger the?intensity of the light the better, but the reduction has a cumulative effect so even not very strong intensity bulbs can be effective. Ren? J. --- On Tue, 4/12/11, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Von kossa stain To: "'histonet@lists.utsouthwestern.edu'" Date: Tuesday, April 12, 2011, 1:35 PM What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use?? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request.? Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Tue Apr 12 14:41:54 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Apr 12 14:41:58 2011 Subject: [Histonet] Von kossa stain In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> Message-ID: I purchase a kit from Dorn and Hart Microedge and use sodium carbonate-formaldehyde solution to chemically develop. Stain in silver nitrate for 5 min in the dark, 3 fresh DI rinses for 1 min each in the dark, then sodium carbonate-formaldehyde solution for 2 min in the dark, 2 fresh DI rinses (normal light) for 1 min each, 30 seconds in Farmer's Diminisher (Sodium thiosulfate-potassium ferricyanide soluiton to stop reaction) and a running tap water rinse for 10 min. Jack > From: Dorothy.L.Webb@HealthPartners.Com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 12:35:18 -0500 > Subject: [Histonet] Von kossa stain > > What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Apr 12 18:11:35 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 12 18:11:55 2011 Subject: [Histonet] Counterstain for dual chromogenic In-Reply-To: <4DA471AD.3040002@georgetown.edu> References: <4DA471AD.3040002@georgetown.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E925@xmdb02.nch.kids> Try ethyl green: 1. 0.1N Acetic Acid Add 6ml of glacial acetic acid to 1000 ml of distilled water. 2. 0.1N Sodium Acetate Add 4.102 g of sodium acetate to 500 ml of distilled water 3. pH 4.2 buffer Add 755 ml of 0.1N acetic acid to 264 ml of 0.1N Sodium Acetate and adjust the pH to 4.2 with 1M NaOH 4. Ethyl Green Solution To 100ml of buffer add 2g Ethyl Green (CI 42590) Can be stored at room temp. If staining turns bluish than the solution has started to deteriorate. Stain for 5 minutes. Do not rinse in water for too long, it tends to extract the green staining Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Wednesday, 13 April 2011 1:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counterstain for dual chromogenic Good morning, I have been attempting to do some dual chromogenic staining. I am using a mouse and a rabbit antibody. For the mouse antibody I am using an HRP-mouse secondary followed by AEC for visualization. For the rabbit antibody I am using a biotinylated rabbit secondary, followed by ABC-AP and vector blue. My problem is what to use as a counterstain. From everything I have read so far there isn't one that would work without having some problems. I was thinking of trying a very light Hematoxylin and see if it doesn't disrupt the vector blue visualization too much. Does anyone else have any suggestions? Thanks, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Tue Apr 12 18:22:21 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 12 18:22:40 2011 Subject: [Histonet] Von kossa stain In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E477BD@nmdamailsvr.nmda.ad.nmsu.edu> References: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> <4D14F0FC9316DD41972D5F03C070908B02E477BD@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E95A@xmdb02.nch.kids> Interesting, Meloan & Puchtler (1985) have drawn our attention to Von Kossa's original work on this technique. He regarded only the yellow colouration of calcium deposits during early stages of the reaction as diagnostic for calcium phosphate and credited the blackening to organic matter. Further studies showed that bright light only causes the irreversible blackening of organic matter that masks the yellow silver phosphate. When the reaction is performed in subdued light, yellow to yellowish brown silver phosphate is visualised selectively. Silver carbonate dissolves in sodium thiosulphate and cannot be demonstrated with von Kossa's technique (Meloan & Puchtler 1985 J Histotechnol 8(1):11-13.). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, 13 April 2011 3:53 AM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Von kossa stain Funny you should ask... just last week I had two requests for a Von Kossa. That's when I found out that, despite the fact that we just moved into a brand new building with all the bells and whistles, there was not one single UV light in any of the many hoods that had been installed. Heaven forbid I could even find an incandescent bulb (we are a "green building"). So, I prepped my slides, put them in a clear glass Coplin jar and parked the jar on the hood of my car for an hour. Works like a charm. This all depends, naturally, on (1) if you even HAVE sunshine where you live; (2) how far a walk it is to the sunshine, and (3) whether you have a car hood on which to park the Coplin jar. Needless to say, out of the line of sight of curious onlookers with sticky fingers and no business wondering what that glass jar is... Nonetheless, it works just fine! May the Force be with you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Tue Apr 12 18:30:30 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 12 18:30:44 2011 Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor In-Reply-To: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60@MAIL-AG-CLUSTER.lha.org> References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60@MAIL-AG-CLUSTER.lha.org> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E981@xmdb02.nch.kids> Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Wednesday, 13 April 2011 4:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is.... Formalin 1 min 37degrees Formalin 15 mins 37degrees 70 14 mins 40 degrees 95 14 mins 40 degrees 95 9 mins 40 degrees 100 9 mins 40 degrees 100 7 mins 40 degrees 100 4 mins 40 degrees Xylene 23 mins no heat Xylene 15 mins no heat Paraffin 20 mins 60 degrees Paraffin 18 mins 60 degrees Paraffin 10 mins 60 degrees Paraffin 0 mins 60 degrees All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges. Any feedback would be greatly appreciated. Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 aevans3@lgheath.org This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From wdesalvo.cac <@t> hotmail.com Tue Apr 12 18:58:36 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Apr 12 18:58:40 2011 Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor In-Reply-To: <6D6BD1DE8A5571489398B392A38A71571884E981@xmdb02.nch.kids> References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60@MAIL-AG-CLUSTER.lha.org>, <6D6BD1DE8A5571489398B392A38A71571884E981@xmdb02.nch.kids> Message-ID: I would consider replacing the sponges. Tony is correct that you need to make sure the air is removed from the sponge to facilitate exchange, but with shortened processing times you will undoubtedly cause carry over from solution to solution. Yuo may want to have a small container of alcoholic formalin with sponges sitting on the gross table. Try using a nylon tissue bag to funnel filter your small biopsies. all your biopsy sample sizes can be placed into the bag and very tiny samples are retained. Last suggestion is to consider a folded filter paper method (we name it origami, I can provide directly if you want) that uses a quick four fold process w/ forceps at the gross bench to create a pocket for the tiny samples, has a single layer between the tissue/solution and is easy to open at embedding (without "popping" of tissue samples). Avoind the unnecessary carryover of the sponge if you can. William DeSalvo, B.S., HTL(ASCP) > From: AnthonyH@chw.edu.au > To: aevans3@lghealth.org; histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 23:30:30 +0000 > CC: > Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor > > Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick). > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B > Sent: Wednesday, 13 April 2011 4:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor > > Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is.... > > Formalin 1 min 37degrees > Formalin 15 mins 37degrees > 70 14 mins 40 degrees > 95 14 mins 40 degrees > 95 9 mins 40 degrees > 100 9 mins 40 degrees > 100 7 mins 40 degrees > 100 4 mins 40 degrees > Xylene 23 mins no heat > Xylene 15 mins no heat > Paraffin 20 mins 60 degrees > Paraffin 18 mins 60 degrees > Paraffin 10 mins 60 degrees > Paraffin 0 mins 60 degrees > > All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges. > > Any feedback would be greatly appreciated. > > Andria B Evans HTL(ASCP)CM > Lancaster General Hospital > 555 North Duke Street > Lancaster, PA 17604 > (717)544-5511 ext: 77329 > aevans3@lgheath.org > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. > Any unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Apr 12 19:10:04 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 12 19:10:20 2011 Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor In-Reply-To: References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60@MAIL-AG-CLUSTER.lha.org>, <6D6BD1DE8A5571489398B392A38A71571884E981@xmdb02.nch.kids> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E9D3@xmdb02.nch.kids> I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Wednesday, 13 April 2011 9:59 AM To: Tony Henwood; aevans3@lghealth.org; histonet Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor I would consider replacing the sponges. Tony is correct that you need to make sure the air is removed from the sponge to facilitate exchange, but with shortened processing times you will undoubtedly cause carry over from solution to solution. Yuo may want to have a small container of alcoholic formalin with sponges sitting on the gross table. Try using a nylon tissue bag to funnel filter your small biopsies. all your biopsy sample sizes can be placed into the bag and very tiny samples are retained. Last suggestion is to consider a folded filter paper method (we name it origami, I can provide directly if you want) that uses a quick four fold process w/ forceps at the gross bench to create a pocket for the tiny samples, has a single layer between the tissue/solution and is easy to open at embedding (without "popping" of tissue samples). Avoind the unnecessary carryover of the sponge if you can. William DeSalvo, B.S., HTL(ASCP) > From: AnthonyH@chw.edu.au > To: aevans3@lghealth.org; histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 23:30:30 +0000 > CC: > Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor > > Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick). > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B > Sent: Wednesday, 13 April 2011 4:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor > > Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is.... > > Formalin 1 min 37degrees > Formalin 15 mins 37degrees > 70 14 mins 40 degrees > 95 14 mins 40 degrees > 95 9 mins 40 degrees > 100 9 mins 40 degrees > 100 7 mins 40 degrees > 100 4 mins 40 degrees > Xylene 23 mins no heat > Xylene 15 mins no heat > Paraffin 20 mins 60 degrees > Paraffin 18 mins 60 degrees > Paraffin 10 mins 60 degrees > Paraffin 0 mins 60 degrees > > All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges. > > Any feedback would be greatly appreciated. > > Andria B Evans HTL(ASCP)CM > Lancaster General Hospital > 555 North Duke Street > Lancaster, PA 17604 > (717)544-5511 ext: 77329 > aevans3@lgheath.org> > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. > Any unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From polavarapu.mahesh <@t> gmail.com Tue Apr 12 19:25:30 2011 From: polavarapu.mahesh <@t> gmail.com (Mahesh Polavarapu) Date: Tue Apr 12 19:25:34 2011 Subject: [Histonet] Update regarding question on Plastic Embedding Message-ID: Looking for a protocol to visualize vascularization and collagen deposition at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic system. Sections will be rather thick (~50um) b/c they are being made through a titanium anchor. Using MMA with a cold-curing resin, Technovit 9100. Thanks in advance! - Mahesh From Rcartun <@t> harthosp.org Tue Apr 12 19:34:51 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Apr 12 19:34:58 2011 Subject: [Histonet] SP3 HER2 mAb Message-ID: <4DA4B76A.7400.0077.1@harthosp.org> Anyone using the HER2 rabbit monoclonal antibody (clone SP3) ever see reactivity with skeletal muscle? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From eugenia902d1 <@t> hotmail.com Tue Apr 12 22:14:30 2011 From: eugenia902d1 <@t> hotmail.com (Eugenia Thomas) Date: Tue Apr 12 22:14:33 2011 Subject: [Histonet] FW: 1 H&E slide vs. 2 In-Reply-To: References: Message-ID: From: eugenia902d1@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: 1 H&E slide vs. 2 Date: Mon, 11 Apr 2011 13:06:59 -0400 Good afternoon everyone, Does anyone know of an article or statistics discussing the impact (medical diagnosing) of cutting 2 H&E slides per block verses 1 for all routine work? Genia From Barbara.Crill <@t> LPNT.net Wed Apr 13 08:39:22 2011 From: Barbara.Crill <@t> LPNT.net (Barbara.Crill@LPNT.net) Date: Wed Apr 13 08:39:35 2011 Subject: [Histonet] Ventana ultra Message-ID: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net From PMonfils <@t> Lifespan.org Wed Apr 13 09:00:09 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Apr 13 09:00:19 2011 Subject: [Histonet] FW: 1 H&E slide vs. 2 In-Reply-To: References: Message-ID: <4EBFF65383B74D49995298C4976D1D5E08020E55@LSRIEXCH1.lsmaster.lifespan.org> When I was in clinical histology (I'm in research now) the pathologists didn't even want us putting two sections on a slide, because even though they knew a second serial section wasn't going to show anything the first section didn't show, they felt ethically/legally bound to examine both sections if they were there, so they were investing twice the time and effort for the same return. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eugenia Thomas Sent: Tuesday, April 12, 2011 11:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: 1 H&E slide vs. 2 From: eugenia902d1@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: 1 H&E slide vs. 2 Date: Mon, 11 Apr 2011 13:06:59 -0400 Good afternoon everyone, Does anyone know of an article or statistics discussing the impact (medical diagnosing) of cutting 2 H&E slides per block verses 1 for all routine work? Genia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Apr 13 09:09:22 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Apr 13 09:09:26 2011 Subject: [Histonet] Ventana ultra In-Reply-To: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> References: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> Message-ID: Please "Reply All" as I'd like to hear feedback also. Thanks. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Wednesday, April 13, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcates <@t> synecor.com Wed Apr 13 09:13:23 2011 From: lcates <@t> synecor.com (Lynne Cates) Date: Wed Apr 13 09:13:32 2011 Subject: [Histonet] Renal Catecholamines Message-ID: Does anyone know any places that perform catecholamines on porcine renal tissue and contact numbers? Lynne From kim.tournear <@t> yahoo.com Wed Apr 13 09:32:56 2011 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Wed Apr 13 09:33:04 2011 Subject: [Histonet] Ventana ultra In-Reply-To: Message-ID: <504386.23697.qm@web120220.mail.ne1.yahoo.com> I've heard that adding slides and/or reagents can add as much as 8 minutes to the run time. Again, that's just what I've heard, don't know if it's fact or fiction.... ~Kim~? ? OU ROCKS!!!! ~Don't be afraid your life will end, be afraid it will never begin~ --- On Wed, 4/13/11, Sebree Linda A wrote: From: Sebree Linda A Subject: RE: [Histonet] Ventana ultra To: Barbara.Crill@LPNT.net, histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 2:09 PM Please "Reply All" as I'd like to hear feedback also. Thanks. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Wednesday, April 13, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail:? barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Apr 13 09:41:42 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Apr 13 09:41:56 2011 Subject: [Histonet] Ventana ultra In-Reply-To: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> References: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> Message-ID: <4DA57DE6.2B7F.00C9.1@geisinger.edu> each of the 30 drawers essentially is it's own "run". You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> 4/13/2011 9:39 AM >>> Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From gu.lang <@t> gmx.at Wed Apr 13 09:44:27 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Apr 13 09:44:46 2011 Subject: AW: [Histonet] Benchmark Ultra question - follow up In-Reply-To: References: <3F8B4FBC9BAF43628DFAE0F32E7C3BDA@dielangs.at><4DA2EE9A.2B7F.00C9.1@geisinger.edu>, <881AD8F57F7B4ECF84F1411A606A68C4@dielangs.at>, <846C2F83097F4C4C935C3BB8E7A6B215@dielangs.at>, Message-ID: <87A14A80DD074AA5A3308A1E3BDD40BA@dielangs.at> Thank you all for your replies. After all I have to admit, that the cause was human failure. We found, that the protocols have been changed and noone did remember. That was after a stopped run, where HIER war clicked off for the repetition. And afterward only short HIER was clicked on again instead of mild HIER. ? stupid me! Bye Gudrun _____ Von: Lynn Lee [mailto:lynnlee2010@live.com] Gesendet: Mittwoch, 13. April 2011 04:14 An: gu.lang@gmx.at Betreff: FW: [Histonet] Benchmark Ultra question - follow up One last thought- do you filter the new antibody solution you made EVERY time when refilling the prep kit? If not, there could be some particulates in the diluent causing a blockage also. Hope these suggestions help or maybe you have already checked this out. Lynn Lee _____ From: lynnlee2010@live.com To: gu.lang@gmx.at Subject: RE: [Histonet] Benchmark Ultra question - follow up Date: Tue, 12 Apr 2011 20:10:43 -0600 It's possible the flip lid on the prep kit dispenser top is not completed closed and seated properly. When the dispense hammer goes down, the pressure may not be the same, every time if this is the case. I always check the tip of the dispenser for air bubbles before putting on the machine for EVERY one whether it is a prefilled Ventana dispenser or my prep kit dispenser. Finally something could be clogged in a line somewhere. I worked in R & D for Ventana for almost 4 years and have since used the XT and Ultra in other labs and never had a problem with incorrect amount of reagent being dispensed. I guess there's a first time for anything though. I would call Ventana Customer Support. They are great! Lynn Lee B.S., HT(ASCP) Tucson, AZ > From: gu.lang@gmx.at > To: histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 18:23:27 +0200 > Subject: [Histonet] Benchmark Ultra question - follow up > > Today I made a further experiment. I did two titration runs. On one slide I > added the working solution by pipette on the other slide I added it by > pushing the filled PrepKit. > Both stainings came out beautiful. - There has to be something wrong with > the dispension-amount. Ventana is informed, I hope they find the culprit. > > Bye > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun > Lang > Gesendet: Montag, 11. April 2011 18:48 > An: 'Angela Bitting' > Cc: histonet@lists.utsouthwestern.edu > Betreff: AW: [Histonet] Benchmark Ultra question > > Yes, that could be true. Some of the PrepKits "go harder" than others. > Perhaps the pressure of the hammer is not big enough. Sometimes the nozzles > are not filled equally. (We press always reagens in the nozzle when we > prepare the run.) > > With the Ventana-System there is an additional dilution, because the > working-solution is added to a reaction-buffer film under the LCS. Perhaps > it makes a difference if you dispense the solution with the pipett-tip under > the LCS or if the drop falls on the surface of the LCS. That could be a > matter of the LCS-quality, or buffer-quantity , or . I think I'll become > mad! > > Our pathologists have an uncertain unhappiness with the overall staining > results, but most of the antibodies work well. Perhaps the majority isn't > very sensitiv to small changes in the system and the quality-difference > isn't big enough, but some are easier to "kill". > > Regards, Gudrun > > > > > > _____ > > Von: Angela Bitting [mailto:akbitting@geisinger.edu] > Gesendet: Montag, 11. April 2011 18:06 > An: gu.lang@gmx.at > Betreff: Re: [Histonet] Benchmark Ultra question > > > > When I was trained to do titration runs on the BenchmarkXT and Ultras, I was > told to titrate 100ul of antibody. I have been suspecting for some time now > that the dispensers DON'T dispense a full 100ul. I haven't taken the time to > prove it, but your question may have motivated me. > > That could explain the weak staining. > > >>> "Gudrun Lang" 4/11/2011 11:48 AM >>> > Hi! > > I have some issues with our new Ultra. Since a few days antibodies, that > work usually well, show up very faint. For example the TTF1 (Novocastra). > > I ordered a new bottle, made a titration and found, that the "old" titer > 1:50 was again well enough. I filled the old PrepKit - and the result was > very weak. > > Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - > and again the result was very weak. At that time the working-solution was > only three days old. > > > > Has anyone an explanation, why the titration works well and the automated > dispension doesn't? > > > > Regards > > Gudrun Lang > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _____ > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It is > intended solely for the addressee. Access to this message by anyone else is > unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Kuhnla <@t> chsli.org Wed Apr 13 09:45:06 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Apr 13 09:45:15 2011 Subject: [Histonet] Ventana ultra In-Reply-To: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> References: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> Message-ID: I currently have one XT and 3 Ultras. I love the Ultras. There is no difference in reagent costs. The only thing that I can say remotely related is that if you keep both platforms, the XT and the ultras have separate CC1, CC2, and LCS. I am fairly certain the contents of the bottle are the same, but the label and part numbers are different. I love the continuous access. I have it set up where the three ultras are 'top loaded' with set antibodies every day. This eliminates bouncing things around all the time. Contact me if you would like anymore info. I would be happy to help. I notice no delay in run times. melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Wednesday, April 13, 2011 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From rjbuesa <@t> yahoo.com Wed Apr 13 09:52:04 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 13 09:52:08 2011 Subject: [Histonet] FW: 1 H&E slide vs. 2 In-Reply-To: Message-ID: <748035.62239.qm@web65706.mail.ac4.yahoo.com> There is not such a study. Cutting 2 vs. 1 H&E/block is merely a preference issue amongst pathologists. Now, if you refer to TWO different levels, that is a different issue. Two consecutive-serial sections from each block should not have a diagnostic impact BUT two different levels, separated by 20-25 sections, may. That is a decision to take by the pathologist. Ren? J. --- On Tue, 4/12/11, Eugenia Thomas wrote: From: Eugenia Thomas Subject: [Histonet] FW: 1 H&E slide vs. 2 To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 12, 2011, 11:14 PM From: eugenia902d1@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: 1 H&E slide vs. 2 Date: Mon, 11 Apr 2011 13:06:59 -0400 Good afternoon everyone, Does anyone know of an article or statistics discussing the impact (medical diagnosing) of cutting 2 H&E slides per block verses 1 for all routine work? Genia ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Wed Apr 13 09:55:38 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Apr 13 09:55:37 2011 Subject: [Histonet] Ventana ultra In-Reply-To: References: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B370407E57C@giamail2.Gia.com> I recently got an Ultra about a month ago and doing test runs, I have really enjoyed the continuous feed as well but I have a question...if I deactivate a product from the Ultra's inventory list I notice it's still in my XT's inventory list. So, I have to deactivate everything off of each instrument? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, April 13, 2011 9:45 AM To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana ultra I currently have one XT and 3 Ultras. I love the Ultras. There is no difference in reagent costs. The only thing that I can say remotely related is that if you keep both platforms, the XT and the ultras have separate CC1, CC2, and LCS. I am fairly certain the contents of the bottle are the same, but the label and part numbers are different. I love the continuous access. I have it set up where the three ultras are 'top loaded' with set antibodies every day. This eliminates bouncing things around all the time. Contact me if you would like anymore info. I would be happy to help. I notice no delay in run times. melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Wednesday, April 13, 2011 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Wed Apr 13 09:54:44 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Wed Apr 13 09:55:46 2011 Subject: [Histonet] Ventana ultra In-Reply-To: <4DA57DE6.2B7F.00C9.1@geisinger.edu> References: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> <4DA57DE6.2B7F.00C9.1@geisinger.edu> Message-ID: <7111DB39D045004C9CF29E79C71B28BC101D507915@CMHEXCC01MBX.childrensmemorial.org> We like ours as well. It's not as continual as we originally thought.... to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a "landing zone" in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run). Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 13, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu; Barbara.Crill@LPNT.net Subject: Re: [Histonet] Ventana ultra each of the 30 drawers essentially is it's own "run". You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> 4/13/2011 9:39 AM >>> Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From gu.lang <@t> gmx.at Wed Apr 13 10:01:35 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Apr 13 10:01:53 2011 Subject: AW: [Histonet] Ventana ultra In-Reply-To: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> References: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> Message-ID: <49E6A03ED68944AEA42E8F4F115F9012@dielangs.at> What we like about the Ultra is, that there's no need for cleaning runs between the runs and that you can take out the bulk and waste containers during the run. We don't use the continious loading, because it doesn't fit to our workplan and because we use various antibodies from run to run. The runs are faster, but more than 2 runs per 8 hour shift and 1 run over night are not possible for us. That's the same workload we did with the XT. There are "windows" during the run, when you can add new slides and reagens. If the needed reagens in on the instrument, you can add them any time, if there's enough place. But the run will last longer and you have to wait for a "window" of the longer run to get the reagens off the instrument. Ready stained slides can be taken out independently. But the free pads cannot be filled and runs started, until you have the possibility to add the right reagens. So we decided to stay with "patch-workload" and real go-away. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Barbara.Crill@LPNT.net Gesendet: Mittwoch, 13. April 2011 15:39 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Wed Apr 13 10:12:18 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Apr 13 10:18:25 2011 Subject: [Histonet] RE: Histonet Digest, Von Kossa Stain In-Reply-To: References: Message-ID: We used the lamp at our cutting stations, coplin jar in our waterbath or on top of the embedder, wrapped in tin foil for 1 hr. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ------------------------------ From plucas <@t> biopath.org Wed Apr 13 10:29:39 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed Apr 13 10:25:56 2011 Subject: [Histonet] IHC platforms/Sales Representatives Message-ID: <6619BD7E2C004B4FA2E53529F39EB9B1@biopath.local> Hello, We are inquiring about IHC staining platforms and we've already contacted the Leica sales rep for the Bond. I would also like to contact Ventana to get a quote on their system, and I've tried to get in contact with a sales rep in our area but I haven't had anyone returning my calls. If there is a Vantana rep reading this, would you please contact the sales representative for my area and let him/her know we are interested in a quote? We are located in Orange County (Fountain Valley), California. For those who have experience with these two platforms, would you mind taking the time to share with me your views and experiences about the two systems? Thanks so much, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 714.433.1330 From akbitting <@t> geisinger.edu Wed Apr 13 10:30:43 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Apr 13 10:30:54 2011 Subject: AW: [Histonet] Benchmark Ultra question - follow up In-Reply-To: <87A14A80DD074AA5A3308A1E3BDD40BA@dielangs.at> References: <3F8B4FBC9BAF43628DFAE0F32E7C3BDA@dielangs.at><4DA2EE9A.2B7F.00C9.1@geisinger.edu>, <881AD8F57F7B4ECF84F1411A606A68C4@dielangs.at>, <846C2F83097F4C4C935C3BB8E7A6B215@dielangs.at>, <87A14A80DD074AA5A3308A1E3BDD40BA@dielangs.at> Message-ID: <4DA58963.2B7F.00C9.1@geisinger.edu> Augh! I hate when that happens! >>> "Gudrun Lang" 4/13/2011 10:44 AM >>> Thank you all for your replies. After all I have to admit, that the cause was human failure. We found, that the protocols have been changed and noone did remember. That was after a stopped run, where HIER war clicked off for the repetition. And afterward only short HIER was clicked on again instead of mild HIER. ? stupid me! Bye Gudrun _____ Von: Lynn Lee [mailto:lynnlee2010@live.com] Gesendet: Mittwoch, 13. April 2011 04:14 An: gu.lang@gmx.at Betreff: FW: [Histonet] Benchmark Ultra question - follow up One last thought- do you filter the new antibody solution you made EVERY time when refilling the prep kit? If not, there could be some particulates in the diluent causing a blockage also. Hope these suggestions help or maybe you have already checked this out. Lynn Lee _____ From: lynnlee2010@live.com To: gu.lang@gmx.at Subject: RE: [Histonet] Benchmark Ultra question - follow up Date: Tue, 12 Apr 2011 20:10:43 -0600 It's possible the flip lid on the prep kit dispenser top is not completed closed and seated properly. When the dispense hammer goes down, the pressure may not be the same, every time if this is the case. I always check the tip of the dispenser for air bubbles before putting on the machine for EVERY one whether it is a prefilled Ventana dispenser or my prep kit dispenser. Finally something could be clogged in a line somewhere. I worked in R & D for Ventana for almost 4 years and have since used the XT and Ultra in other labs and never had a problem with incorrect amount of reagent being dispensed. I guess there's a first time for anything though. I would call Ventana Customer Support. They are great! Lynn Lee B.S., HT(ASCP) Tucson, AZ > From: gu.lang@gmx.at > To: histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 18:23:27 +0200 > Subject: [Histonet] Benchmark Ultra question - follow up > > Today I made a further experiment. I did two titration runs. On one slide I > added the working solution by pipette on the other slide I added it by > pushing the filled PrepKit. > Both stainings came out beautiful. - There has to be something wrong with > the dispension-amount. Ventana is informed, I hope they find the culprit. > > Bye > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun > Lang > Gesendet: Montag, 11. April 2011 18:48 > An: 'Angela Bitting' > Cc: histonet@lists.utsouthwestern.edu > Betreff: AW: [Histonet] Benchmark Ultra question > > Yes, that could be true. Some of the PrepKits "go harder" than others. > Perhaps the pressure of the hammer is not big enough. Sometimes the nozzles > are not filled equally. (We press always reagens in the nozzle when we > prepare the run.) > > With the Ventana-System there is an additional dilution, because the > working-solution is added to a reaction-buffer film under the LCS. Perhaps > it makes a difference if you dispense the solution with the pipett-tip under > the LCS or if the drop falls on the surface of the LCS. That could be a > matter of the LCS-quality, or buffer-quantity , or . I think I'll become > mad! > > Our pathologists have an uncertain unhappiness with the overall staining > results, but most of the antibodies work well. Perhaps the majority isn't > very sensitiv to small changes in the system and the quality-difference > isn't big enough, but some are easier to "kill". > > Regards, Gudrun > > > > > > _____ > > Von: Angela Bitting [mailto:akbitting@geisinger.edu] > Gesendet: Montag, 11. April 2011 18:06 > An: gu.lang@gmx.at > Betreff: Re: [Histonet] Benchmark Ultra question > > > > When I was trained to do titration runs on the BenchmarkXT and Ultras, I was > told to titrate 100ul of antibody. I have been suspecting for some time now > that the dispensers DON'T dispense a full 100ul. I haven't taken the time to > prove it, but your question may have motivated me. > > That could explain the weak staining. > > >>> "Gudrun Lang" 4/11/2011 11:48 AM >>> > Hi! > > I have some issues with our new Ultra. Since a few days antibodies, that > work usually well, show up very faint. For example the TTF1 (Novocastra). > > I ordered a new bottle, made a titration and found, that the "old" titer > 1:50 was again well enough. I filled the old PrepKit - and the result was > very weak. > > Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - > and again the result was very weak. At that time the working-solution was > only three days old. > > > > Has anyone an explanation, why the titration works well and the automated > dispension doesn't? > > > > Regards > > Gudrun Lang > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _____ > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It is > intended solely for the addressee. Access to this message by anyone else is > unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Apr 13 10:36:20 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Apr 13 10:36:30 2011 Subject: [Histonet] Ventana ultra In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC101D507915@CMHEXCC01MBX.childrensmemorial.org> References: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> <4DA57DE6.2B7F.00C9.1@geisinger.edu> <7111DB39D045004C9CF29E79C71B28BC101D507915@CMHEXCC01MBX.childrensmemorial.org> Message-ID: <4DA58AB4.2B7F.00C9.1@geisinger.edu> We load our Ultras with the most commonly used antibodies each morning. So that minimizes the number of landing zones we need to use. Also, (if you run both XTs and Ultras) run your antibodies with the longest protocols on Ultra and that will shorten your XT runs. >>> "Setlak, Lisa" 4/13/2011 10:54 AM >>> We like ours as well. It's not as continual as we originally thought.... to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a "landing zone" in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run). Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 13, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu; Barbara.Crill@LPNT.net Subject: Re: [Histonet] Ventana ultra each of the 30 drawers essentially is it's own "run". You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> 4/13/2011 9:39 AM >>> Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From dwenzel01 <@t> gmail.com Wed Apr 13 11:16:48 2011 From: dwenzel01 <@t> gmail.com (Dawn Herron) Date: Wed Apr 13 11:16:55 2011 Subject: [Histonet] slide brite and coverslip drying time Message-ID: Hello all. We have just made the transition from xylene in our linear stainer to Slide Brite. We are also coverslipping from Slide Brite with Permount. So far the slides look good (though we have to be careful about water contamination in the dehydrating alcohols) but we are having a problem with extremely long coverslipping drying time. Some of the slides have been on the warmer for over 18 hours and you can still move the coverslips! (Normally the slides would dry within 4 hours on the warmer with xylene and acrymount) Any suggestions on how to speed drying time? Thanks, Dawn From jqb7 <@t> cdc.gov Wed Apr 13 12:06:59 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Apr 13 12:07:06 2011 Subject: [Histonet] Tissue transfer Message-ID: Hello all, We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions? Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From rnegron <@t> sarapath.com Wed Apr 13 12:32:57 2011 From: rnegron <@t> sarapath.com (Robin Negron) Date: Wed Apr 13 12:43:02 2011 Subject: [Histonet] General Data Cassette Labeler Message-ID: Can anyone that uses the General Data cassette labeler give me information regarding your overall opinion of this instrument. 1. I would like to have any information regarding the speed of machine. When we did a demo of this equipment it seems fast. 2. I was also questioning the cassettes, we have found that the only company to purchase these from is general data (at a high cost). 3. Has anyone had the equipment break down and how is the dependability and the customer support service. 4. Our company is very interested in the on demand version. How has anyone found this to be an advantage. 5. Does anyone purchase the cassettes from another dealer besides General Data 6. Has anyone had problems with the black scatching off. 7. We currently use TBS and they are very slow and constantly break down this does not meet our demand as we currently print approx. 400-500 per day. I would like to hear your opinions. From christina.thurby <@t> bms.com Wed Apr 13 13:04:56 2011 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Wed Apr 13 13:05:04 2011 Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? In-Reply-To: References: Message-ID: Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From Diane.Craft <@t> amcny.org Wed Apr 13 13:11:38 2011 From: Diane.Craft <@t> amcny.org (Diane.Craft@amcny.org) Date: Wed Apr 13 13:05:28 2011 Subject: [Histonet] Tissue transfer In-Reply-To: References: Message-ID: I use Harleco Krystalon liquid coverslip medium. Cover the tissue, laying horizontally in oven for a few hours until it hardens, than soak in water bath, peel, and transfer to charged slide with same side facing down, dry in oven again, dissolve in xylene when ready for staining. Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) "Bartlett, Jeanine (CDC/OID/NCEZID)" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/13/2011 01:06 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Tissue transfer Hello all, We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions? Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Wed Apr 13 14:31:09 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Apr 13 14:31:15 2011 Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? In-Reply-To: References: Message-ID: As long as possible. I remember reading somewhere about TB in lung tissue that was still viable after *years* of 10% NBF. Make sure the lungs are thoroughly perfused, or they will still be unfixed in the middle. There is a technique to "inflate" lungs at autopsy using a large syringe full of NBF and a clamp. Make sure that the specimen is not contacting the walls or bottom of the specimen container. You can suspend the whole organ with string inside the container, if needed. One can also use gauze or paper towels to keep the organ under the surface of the fixative. Lung tissue is notoriously difficult to fix properly, due both to the high lipid content of the pleural parenchyma, and the fact that fresh lungs float. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From rjbuesa <@t> yahoo.com Wed Apr 13 14:51:07 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 13 14:51:11 2011 Subject: [Histonet] slide brite and coverslip drying time In-Reply-To: Message-ID: <237269.18418.qm@web65705.mail.ac4.yahoo.com> That is exactly one of the problems with Slide Brite, and is the "trade-off" you have to pay. The alternative (although it may seem "odd") is to get away with alcohols and xylene altogether. After staining the slides, wash them in distilled water, shake them well?and place them in an over at 60?C for 5 minutes. After drying apply the mounting medium more diluted (consistency of light mineral oil), and coverslip. You will get away with alcohols, and xylene (or its substitutes). Do not "frown", just try it! Ren? J. --- On Wed, 4/13/11, Dawn Herron wrote: From: Dawn Herron Subject: [Histonet] slide brite and coverslip drying time To: Histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 12:16 PM Hello all. We have just made the transition from xylene in our linear stainer to Slide Brite. We are also coverslipping from Slide Brite with Permount. So far the slides look good (though we have to be careful about water contamination in the dehydrating alcohols) but we are having a problem with extremely long coverslipping drying time. Some of the slides have been on the warmer for over 18 hours and you can still move the coverslips! (Normally the slides would dry within 4 hours on the warmer with xylene and acrymount) Any suggestions on how to speed drying time? Thanks, Dawn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Apr 13 14:57:28 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 13 14:57:32 2011 Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? In-Reply-To: Message-ID: <632902.43444.qm@web65712.mail.ac4.yahoo.com> 5?days (96 h) is more than enough, but always handle the tissue and blocks?using safety precautions. Ren? J. --- On Wed, 4/13/11, Thurby, Christina wrote: From: Thurby, Christina Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, April 13, 2011, 2:04 PM Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case?? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information.? The information is intended to be for the use of the individual or entity designated above.? If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments.? Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bnasreena <@t> gmail.com Wed Apr 13 15:07:43 2011 From: bnasreena <@t> gmail.com (Nasreen Bashir) Date: Wed Apr 13 15:07:46 2011 Subject: [Histonet] DAB staining for Liver frozen sections Message-ID: Hi, I need some information on how to block endogenous peroxidase effectively in liver frozen sections, as I need to do DAB staining. What blocking buffer will work best for blocking in liver frozen sections. I appreciate any information on this issue. Thanks, Reena From KumarD <@t> mail.amc.edu Wed Apr 13 15:08:33 2011 From: KumarD <@t> mail.amc.edu (Kumar, Devender) Date: Wed Apr 13 15:08:42 2011 Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? In-Reply-To: <632902.43444.qm@web65712.mail.ac4.yahoo.com> References: <632902.43444.qm@web65712.mail.ac4.yahoo.com> Message-ID: Hi, We fix 'Category-A' agent infected murine lungs in 10% buffered formalin for 7 days in ABSL-3. We also confirm tissue sterility by plating the tissue homogenates on suitable media before taking the samples out of ABSL-3 facility for sectioning. In your case 7 days should be enough, but it is always better to confirm the sterility of tissue. Thanks, Devender Devender Kumar, D.V.M., Ph.D. Postdoctorate fellow, Center for Immunology & Microbial Disease, Albany Medical College, 47 New Scotland Avenue, MC 151 Albany, NY 12208 518.262.6220(LAB) -- -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 13, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu; ChristinaThurby Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? 5?days (96 h) is more than enough, but always handle the tissue and blocks?using safety precautions. Ren? J. --- On Wed, 4/13/11, Thurby, Christina wrote: From: Thurby, Christina Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, April 13, 2011, 2:04 PM Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case?? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information.? The information is intended to be for the use of the individual or entity designated above.? If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments.? Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From rjbuesa <@t> yahoo.com Wed Apr 13 14:54:12 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 13 15:09:05 2011 Subject: [Histonet] Tissue transfer In-Reply-To: Message-ID: <899879.15372.qm@web65713.mail.ac4.yahoo.com> "Mission impossible" (successfully, you can always ruin them)! It is better to do the staining after a prolonged oven heating and do it delicately. Ren? J. --- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) Subject: [Histonet] Tissue transfer To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, April 13, 2011, 1:06 PM Hello all, We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing.? Any suggestions? Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA? 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Norm.Burnham <@t> propath.com Wed Apr 13 16:52:58 2011 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Wed Apr 13 16:53:03 2011 Subject: [Histonet] Part-time Histotech Opening Message-ID: <82C7248978CB50469FD6BA68EBBEFE6705653652@exchange.propathlab.com> Dear Histonetters, We have the following opening: HISTOTECHNOLOGIST ProPath, a high volume, pathology practice, located in Dallas, Texas, has an immediate opening for a part-time Histotechnologist. Responsibilities include embedding tissue specimens, microtomy of paraffin-embedded tissue, operation of automated stainer and coverslipper, equipment maintenance and record retention. The ideal candidate will have a high school diploma or equivalent. We prefer, HT, HTL (ASCP) registered or eligible. The hours are 2:00 p.m. to 6:00 p.m. Monday - Friday. For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 FAX: 214/237-1825 Job-Line: 214/237-1775 Email address: jobs@propath.com Website: www.propath.com Don't Follow the Leader! Join the Leader! EOE From saby_joseph_a <@t> yahoo.com Wed Apr 13 17:51:41 2011 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Wed Apr 13 17:51:45 2011 Subject: [Histonet] Update regarding question on Plastic Embedding In-Reply-To: References: Message-ID: <219317.4678.qm@web114401.mail.gq1.yahoo.com> Mahesh- If you have access to a sonicator, you can etch the slides by first soaking them in 1% formic acid in the sonicator for 5-10 minutes, then after a brief water rinse soak them in 50% ethanol for another 5-10 minutes in the sonicator. You may have to work with the staining times, but you will find that many of your paraffin embedding stains will work. Good luck! Joe Saby, BA HT NAMSA ________________________________ From: Mahesh Polavarapu To: histonet Sent: Tue, April 12, 2011 8:25:30 PM Subject: [Histonet] Update regarding question on Plastic Embedding Looking for a protocol to visualize vascularization and collagen deposition at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic system. Sections will be rather thick (~50um) b/c they are being made through a titanium anchor. Using MMA with a cold-curing resin, Technovit 9100. Thanks in advance! - Mahesh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Wed Apr 13 20:04:50 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Apr 13 20:04:54 2011 Subject: [Histonet] Seeking techs all over the place! Message-ID: <547837.58901.qm@web39422.mail.mud.yahoo.com> Help!? ? We're getting busy again (the economy rebounds??) and need both permanent and temporary histotechs.? ? Are you looking for... ...a new place to live? ...a bigger?city? ...a smaller city? ...a promotion? ...a raise? ...a?place to learn new things? ? We will actively consider... ...new grads?(and almost grads) ...experienced bench techs ...bench techs looking for a step up ...experienced management ...retirees (we know you've still 'got it') ? We have a number of permanent openings and seeking to successfully fill 9 thirteen-week temp positions.? We are working histotechs so the conversation is easy and usually kinda fun. Send a resume or ask for an application - tkngflght@yahoo.com Fax/Phone: 800.756.3309 From Fawaz.Zouabi <@t> sswahs.nsw.gov.au Wed Apr 13 22:44:54 2011 From: Fawaz.Zouabi <@t> sswahs.nsw.gov.au (Fawaz Zouabi) Date: Wed Apr 13 22:45:52 2011 Subject: [Histonet] Histology Supervisor ( Hospital Scientist)-F/T Message-ID: A Histology Supervisor ( Hospital Scientist) -F/T position is currently available at The Department of Forensic Medicine (Sydney local health network / Sydney south West), GLEBE 2037 NSW AUSTRALIA. To enquire please check the NSW health web site on http://www.health.nsw.gov.au/jobs/index.asp The successful applicant will be responsible of the supervision of the DOFM histology laboratory which involves the collection and processing of forensic histological specimens. From AnthonyH <@t> chw.edu.au Thu Apr 14 01:58:57 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Apr 14 01:59:14 2011 Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71571884F5E6@xmdb02.nch.kids> Christina, The following might be useful: Kappel et al (HUM PATHOL 27:1361--1364, 1996) attempted to grow TB from formalin fixed lung tissue that had previously been shown to be positive by sputum culture. They were unable to culture TB from these tissues. Gerston et al (HUM PATHOL 35:571-575.2004) in South Africa analysed 138 formalin fixed lungs with histological evidence of AFB and were able to culture TB from 12 of these cases (one of these cases had been fixed for 80 days before being tested). Gerston et al suggest that there is a risk of contracting tuberculosis from tissue that has been fixed in formalin, if aerosols or accidental inoculation should occur. Trimming and sectioning wax blocks are of concern but no studies have been done yet. Of concern to histotechnolgists are: 1. Tissue with Inflammation-induced Encapsulation may protect bugs from formalin. 2. Formalin dilutes as it penetrates tissue. 3. Formalin substitutes may not be germicidal. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thurby, Christina Sent: Thursday, 14 April 2011 4:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Melissa.Kuhnla <@t> chsli.org Thu Apr 14 06:11:02 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Thu Apr 14 06:11:07 2011 Subject: [Histonet] Tissue transfer In-Reply-To: <899879.15372.qm@web65713.mail.ac4.yahoo.com> References: <899879.15372.qm@web65713.mail.ac4.yahoo.com> Message-ID: Hello, We do have a successful way to transfer tissue sections. It is using Mount Quik liquid coverslip. We purchase this from Newcomber Supply. Are you doing IHC testing? 1. H&E sections first (so they are visible)(for IHC, retrieval will fade the stain anyways) 2. After the stain, run slide to Xylene 3. Cover entire slide with layer of Mount Quik 4. Bake horizontal in 60 degree oven for 1 hour 5. Bake vertical for one hour 6. Rinse slide in running warm water until the mount quik and tissue lift from the slide 7. Divide the tissue as need, place portions on plus slide 8. Bake horizontal for 1 hour 9. Bake vertical for one hour 10. Dissolve off mount quik with several 10 minute steps in Xylene. No visible residual should be left 11. Carry out testing Hope this helps Melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 13, 2011 3:54 PM To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett Subject: Re: [Histonet] Tissue transfer "Mission impossible" (successfully, you can always ruin them)! It is better to do the staining after a prolonged oven heating and do it delicately. Ren? J. --- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) Subject: [Histonet] Tissue transfer To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, April 13, 2011, 1:06 PM Hello all, We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing.? Any suggestions? Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA? 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From darlene.giracello <@t> okstate.edu Thu Apr 14 09:19:00 2011 From: darlene.giracello <@t> okstate.edu (Giracello, Darlene) Date: Thu Apr 14 09:19:06 2011 Subject: [Histonet] Cassette labeling Message-ID: <0C2BDF59DC80E64AB2A59939D703B2E5016BD5C2EEC1@STWEXE3.ad.okstate.edu> Our cassette printer finally gave up on us, and we are looking at available options. Does anyone have experience with the Brady cassette labeling system? We would really appreciate ANY feedback about this product. We are also open to suggestions. We are a veterinary lab with budget constraints :-) Thank you in advance for your help Darlene From amosbrooks <@t> gmail.com Thu Apr 14 09:25:16 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Apr 14 09:25:23 2011 Subject: [Histonet] AKT anyone? Message-ID: Hi, I am trying to get AKT working on formalin fixed paraffin embedded human surgical pathology tissue. I have tried two vendors (Cell Signalling and Epitomics) and I'm having terrible difficulty getting anything out of it. I was wondering if anyone else is using AKT (from anywhere) and is getting decent results. The PI here is getting really upset about it and I feel like I've done everything but stand on my head while doing the test. Thanks, Amos From east78 <@t> yahoo.co.kr Thu Apr 14 09:07:24 2011 From: east78 <@t> yahoo.co.kr (kyoung LEE) Date: Thu Apr 14 10:02:48 2011 Subject: [Histonet] [Help] oil red O stain in fattty change liver Message-ID: <147665.31524.qm@web70905.mail.kr3.yahoo.com> Dear sirs. I don't know whether I send this question to you. For several weeks, I'm setting oil red O stain in fattty change liver (mouse). Could you review my protocol? 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin). 2. Make frozen block 3. Blocks were cut 5um in crystat and dried for 1~2H at RT.? 4. store at -20 5. let it dry for 30mins at RT 6. immerse it in cold 10% NBF for 10mins 7. staining After staining, my section were fallen?off and seperated. I search many protocols. Some suggest additional fixation is necessary as soon as cutting. Others suggest?prefixed tissue is not?necessary additional fixation. Plz send me your comments. From LSetlak <@t> childrensmemorial.org Thu Apr 14 10:09:41 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Thu Apr 14 10:09:46 2011 Subject: [Histonet] [Help] oil red O stain in fattty change liver In-Reply-To: <147665.31524.qm@web70905.mail.kr3.yahoo.com> References: <147665.31524.qm@web70905.mail.kr3.yahoo.com> Message-ID: <7111DB39D045004C9CF29E79C71B28BC101D50791C@CMHEXCC01MBX.childrensmemorial.org> We do this stain on frozen sections in our lab with no formalin fixation. We stain in oil red o from American Mastertech for 30 minutes, rinse in water, counterstain in hematoxylin, coverslip with aqueous mounting media. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kyoung LEE Sent: Thursday, April 14, 2011 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] [Help] oil red O stain in fattty change liver Dear sirs. I don't know whether I send this question to you. For several weeks, I'm setting oil red O stain in fattty change liver (mouse). Could you review my protocol? 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin). 2. Make frozen block 3. Blocks were cut 5um in crystat and dried for 1~2H at RT.? 4. store at -20 5. let it dry for 30mins at RT 6. immerse it in cold 10% NBF for 10mins 7. staining After staining, my section were fallen?off and seperated. I search many protocols. Some suggest additional fixation is necessary as soon as cutting. Others suggest?prefixed tissue is not?necessary additional fixation. Plz send me your comments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Thu Apr 14 10:56:11 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Apr 14 10:56:15 2011 Subject: [Histonet] need monkey control tissue Message-ID: <0B3AF0D33B2447EF910223B1D49D1226@auxs.umn.edu> Hello, I'm posting this request for a colleague, who is in need of normal monkey placenta (preferably Rhesus). If anyone has some to spare, please let me know and I'll forward your response. Thanks, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) From ttroyer <@t> petersonlab.com Thu Apr 14 11:08:21 2011 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Thu Apr 14 11:08:26 2011 Subject: [Histonet] Activated Carbon Message-ID: <367C74D495C248669E61229AFF3B60EB@Peterson.local> Does anyone have an inexpensive vendor for activated carbon for the processor? Thanks, Travis From JMacDonald <@t> mtsac.edu Thu Apr 14 11:10:33 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Apr 14 11:10:40 2011 Subject: [Histonet] [Help] oil red O stain in fatty change liver In-Reply-To: <147665.31524.qm@web70905.mail.kr3.yahoo.com> Message-ID: We use pre-fixed tissue for oil red o. We rinse the section briefly to remove the formalin from the outside of the tissue. We prepare our tissue in OCT or the equivalent. We cut the frozen sections and mount them on charged slides. We let dry the dry a minimum of 10 minute, rinse them in water to remove the OCT and then proceed with the stain. kyoung LEE Sent by: histonet-bounces@lists.utsouthwestern.edu 04/14/2011 08:04 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] [Help] oil red O stain in fattty change liver Dear sirs. I don't know whether I send this question to you. For several weeks, I'm setting oil red O stain in fattty change liver (mouse). Could you review my protocol? 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin). 2. Make frozen block 3. Blocks were cut 5um in crystat and dried for 1~2H at RT. 4. store at -20 5. let it dry for 30mins at RT 6. immerse it in cold 10% NBF for 10mins 7. staining After staining, my section were fallen off and seperated. I search many protocols. Some suggest additional fixation is necessary as soon as cutting. Others suggest prefixed tissue is not necessary additional fixation. Plz send me your comments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Thu Apr 14 11:10:54 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Apr 14 11:11:45 2011 Subject: [Histonet] Activated Carbon In-Reply-To: <367C74D495C248669E61229AFF3B60EB@Peterson.local> Message-ID: Which processor? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Travis Troyer Sent: Thursday, April 14, 2011 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Activated Carbon Does anyone have an inexpensive vendor for activated carbon for the processor? Thanks, Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From jaylundgren <@t> gmail.com Thu Apr 14 12:04:11 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Apr 14 12:04:14 2011 Subject: [Histonet] Activated Carbon In-Reply-To: <367C74D495C248669E61229AFF3B60EB@Peterson.local> References: <367C74D495C248669E61229AFF3B60EB@Peterson.local> Message-ID: Go to any pet store and by activated carbon for a fish tank filter. Empty out the old filter and replace the carbon. It's messy, but 90% cheaper than buying med supply carbon. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From michelecarr10 <@t> yahoo.com Thu Apr 14 12:36:42 2011 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Thu Apr 14 12:36:45 2011 Subject: [Histonet] hematoxlin hue on slides Message-ID: <236333.29671.qm@web120713.mail.ne1.yahoo.com> Hello everyone, I have a question about hematoxylin hue on the H&E stained slides.? We were not having this problem in the past but all of a sudden have encountered it.? We have changed out the entire solutions on the stainer and checked protocols.? Not sure why we are having this problem. Could it be the slides causing it. Any helpful info would be appreciated. Thanks in advance, Michele Carr Medical Laboratory Services From rcharles <@t> state.pa.us Thu Apr 14 13:56:18 2011 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Thu Apr 14 13:56:22 2011 Subject: [Histonet] un-nucleated squamous epithelial cells Message-ID: <3809C163DC1DA54AA534B3C7794D07B679B741B70E@ENHBGMBX01.PA.LCL> Hello, Has anyone studied where un-nucleated squamous epithelial cells originate from? I'm leaning towards "thumbing blocks" with an ungloved hand but don't know for sure. Other possibilities I've come up with are dandruff from the head or uncovered arms. I hate to see our histologist work in a complete tyvek outfit so I'm searching for clues. Thanks to all. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us From rjbuesa <@t> yahoo.com Thu Apr 14 14:20:31 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 14 14:20:35 2011 Subject: [Histonet] hematoxlin hue on slides In-Reply-To: <236333.29671.qm@web120713.mail.ne1.yahoo.com> Message-ID: <869477.50436.qm@web65705.mail.ac4.yahoo.com> Check the time in the acid alcohol after nuclear staining. Ren? J. --- On Thu, 4/14/11, Michele Carr wrote: From: Michele Carr Subject: [Histonet] hematoxlin hue on slides To: histonet@lists.utsouthwestern.edu Date: Thursday, April 14, 2011, 1:36 PM Hello everyone, I have a question about hematoxylin hue on the H&E stained slides.? We were not having this problem in the past but all of a sudden have encountered it.? We have changed out the entire solutions on the stainer and checked protocols.? Not sure why we are having this problem. Could it be the slides causing it. Any helpful info would be appreciated. Thanks in advance, Michele Carr Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 14 14:22:41 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 14 14:22:44 2011 Subject: [Histonet] un-nucleated squamous epithelial cells In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B679B741B70E@ENHBGMBX01.PA.LCL> Message-ID: <724571.5966.qm@web65713.mail.ac4.yahoo.com> Any extra-dried skin can shed cells. The best option is having your HTs using some skin lotion (like Keri lotion) to prevent shedding of cells. Ren? J. --- On Thu, 4/14/11, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] un-nucleated squamous epithelial cells To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Thursday, April 14, 2011, 2:56 PM Hello, Has anyone studied where un-nucleated squamous epithelial cells originate from????I'm leaning towards "thumbing blocks" with an ungloved hand but don't know for sure.? Other possibilities I've come up with are dandruff from the head or uncovered arms.? I hate to see our histologist work in a complete tyvek outfit so I'm searching for clues. Thanks to all. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Thu Apr 14 14:52:38 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Apr 14 14:52:42 2011 Subject: [Histonet] un-nucleated squamous epithelial cells In-Reply-To: <724571.5966.qm@web65713.mail.ac4.yahoo.com> References: <724571.5966.qm@web65713.mail.ac4.yahoo.com> Message-ID: <248540.17036.qm@web1104.biz.mail.sk1.yahoo.com> If lotion gets in the waterbath, the tissue will not stick to the slides. Shed skin cells can fall from any exposed skin, not just the hands.?Like from the face of those that do not wear make-up or lotion. ? Paula ________________________________ From: Rene J Buesa To: "Histonet (histonet@lists.utsouthwestern.edu)" ; RogerCharles Sent: Thu, April 14, 2011 2:22:41 PM Subject: Re: [Histonet] un-nucleated squamous epithelial cells Any extra-dried skin can shed cells. The best option is having your HTs using some skin lotion (like Keri lotion) to prevent shedding of cells. Ren? J. --- On Thu, 4/14/11, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] un-nucleated squamous epithelial cells To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Thursday, April 14, 2011, 2:56 PM Hello, Has anyone studied where un-nucleated squamous epithelial cells originate from????I'm leaning towards "thumbing blocks" with an ungloved hand but don't know for sure.? Other possibilities I've come up with are dandruff from the head or uncovered arms.? I hate to see our histologist work in a complete tyvek outfit so I'm searching for clues. Thanks to all. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rnegron <@t> sarapath.com Thu Apr 14 14:59:18 2011 From: rnegron <@t> sarapath.com (Robin Negron) Date: Thu Apr 14 14:59:29 2011 Subject: [Histonet] ThermoScientific Printmate Message-ID: Can anyone give me any information Re: Thermo Scientific Printmate? I would like to get general information about this equipment including maintenance experience, dependability, efficiency, and any other experiences you have had either liked or dislike about this machine. Thanks, Robin From victor <@t> pathology.washington.edu Thu Apr 14 17:09:47 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Apr 14 17:10:20 2011 Subject: [Histonet] ThermoScientific Printmate In-Reply-To: References: Message-ID: <4DA770AB.5070608@pathology.washington.edu> Robin, Here is my take on the product from what I have observed. We are a high volume teaching hospital. Pros Cons Size Cost Clean Barcode Service/Support Low Maintenance Cost Can be Networked Service/Support Relatively Fast for Printing in real time Cost Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 4/14/2011 12:59 PM, Robin Negron wrote: > > > Can anyone give me any information Re: Thermo Scientific > Printmate? > > > > I would like to get general information about this > equipment including maintenance experience, dependability, efficiency, > and any other experiences you have had either liked or dislike about > this machine. > > > > Thanks, > > > > Robin > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Fri Apr 15 02:41:29 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Apr 15 02:41:43 2011 Subject: [Histonet] [Help] oil red O stain in fattty change liver In-Reply-To: <147665.31524.qm@web70905.mail.kr3.yahoo.com> References: <147665.31524.qm@web70905.mail.kr3.yahoo.com> Message-ID: We cryoprotect our livers in 20% sucrose overnight at 4C after fixation in 4% paraformaldehyde, then freeze in thawing isopentane (freeze it over liquid nitrogen, lift clear and use it as it melts, but before it has melted completely). Store if necessary at -80C. Section onto charged (Plus) slides and stain in the normal way. I cannot see why yours are coming off, but suggest you try plus slides, then they won't. I can't think that the cryoprotection makes any difference to whether they fall off or not, but I think the sections are easier to cut after cryoprotection than without it. I have been given livers treated both ways. Plus slides: Superfrost plus, available from all good histology suppliers; ours come from VWR. Good luck. Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kyoung LEE Sent: 14 April 2011 15:07 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] [Help] oil red O stain in fattty change liver Dear sirs. I don't know whether I send this question to you. For several weeks, I'm setting oil red O stain in fattty change liver (mouse). Could you review my protocol? 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin). 2. Make frozen block 3. Blocks were cut 5um in crystat and dried for 1~2H at RT.? 4. store at -20 5. let it dry for 30mins at RT 6. immerse it in cold 10% NBF for 10mins 7. staining After staining, my section were fallen?off and seperated. I search many protocols. Some suggest additional fixation is necessary as soon as cutting. Others suggest?prefixed tissue is not?necessary additional fixation. Plz send me your comments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leroyj <@t> upmc.edu Fri Apr 15 07:26:17 2011 From: leroyj <@t> upmc.edu (Le Roy, Jessica) Date: Fri Apr 15 07:28:24 2011 Subject: [Histonet] RE: ThermoScientific Printmate In-Reply-To: References: Message-ID: <07613F6ACF35074FB4CA2CB3A0956CA8136F2C1173@msxmbxnsprd17.acct.upmchs.net> Hello, our department uses this instrument. I asked the specimen processor if she could pass on what she thinks about it. Here is her response: For the most part I really like it. It's a lot quieter than our last cassette printer, and it's much faster. I also like having six functional hoppers because I fill it up a lot less. Also, the trays that it prints into are great and keep us very organized. I feel like it jams up a lot, but it's generally pretty easy to clear the jam and it's just more of a nuisance than anything. Also, four trays (holds a total of 100 cassettes) wasn't quit enough for us and it's a pain to print without a tray (you have to specify a different outlet, and the cassettes shoot all over the place), but extra trays were very expensive and I think you have to buy either two or four at a time. Hope this helps! Lauren I hope this helps, Jessica Le Roy IF Technician UPP Dermatopathology Presbyterian South Tower 200 Lothrop Street, Suite 3725 Pittsburgh, PA 15213 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Negron [rnegron@sarapath.com] Sent: Thursday, April 14, 2011 3:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ThermoScientific Printmate Can anyone give me any information Re: Thermo Scientific Printmate? I would like to get general information about this equipment including maintenance experience, dependability, efficiency, and any other experiences you have had either liked or dislike about this machine. Thanks, Robin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Apr 15 08:27:35 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Apr 15 08:27:38 2011 Subject: [Histonet] ThermoScientific Printmate In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974BBD@is-e2k3.grhs.net> I have had a Printmate for about 3 months now. I think the idea behind the design and how it is suppose to work is fine, but ours has not work correctly since we got it. It continues to jam up and we have had it worked on 3 times now. I think they have decided to replace the unit at this point (most likely with another unit that was sent in for repair and refurbished)! I see little difference in cost to run. We switched cassette vendors and they matched our current price. I think it is worth the added security of patient safety if I can ever get a unit that works. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Negron Sent: Thursday, April 14, 2011 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ThermoScientific Printmate Can anyone give me any information Re: Thermo Scientific Printmate? I would like to get general information about this equipment including maintenance experience, dependability, efficiency, and any other experiences you have had either liked or dislike about this machine. Thanks, Robin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Apr 15 08:42:47 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Apr 15 08:42:57 2011 Subject: [Histonet] ThermoScientific Printmate In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974BBD@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974BBD@is-e2k3.grhs.net> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBBAEFA7D@IBMB7Exchange.digestivespecialists.com> So, is there a cassette printer out there that someone really loves? Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, April 15, 2011 9:28 AM To: Robin Negron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ThermoScientific Printmate I have had a Printmate for about 3 months now. I think the idea behind the design and how it is suppose to work is fine, but ours has not work correctly since we got it. It continues to jam up and we have had it worked on 3 times now. I think they have decided to replace the unit at this point (most likely with another unit that was sent in for repair and refurbished)! I see little difference in cost to run. We switched cassette vendors and they matched our current price. I think it is worth the added security of patient safety if I can ever get a unit that works. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Negron Sent: Thursday, April 14, 2011 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ThermoScientific Printmate Can anyone give me any information Re: Thermo Scientific Printmate? I would like to get general information about this equipment including maintenance experience, dependability, efficiency, and any other experiences you have had either liked or dislike about this machine. Thanks, Robin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Apr 15 08:58:57 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Apr 15 08:59:01 2011 Subject: [Histonet] RELIA Hot Histology Jobs Alert 4/15/2011 Exciting Opportunities in NC, MD, MA IL and OR Message-ID: Hi Histonetters!! I hope everybody is gearing up for a fun packed Spring weekend! I wanted to put up a quick post to tell you about the positions that I am working on and am most excited about. Why am I excited about these positions? Because these clients offer excellent compensation, benefits, relocation assistance and most importantly they are ready to interview and hire ASAP. All of these position are full time permanent positions. The histology positions that I am most excited about are located in these areas: **Baltimore, MD **Portland, OR **Wilmington, NC **Cape Cod, MA **Boston, MA **Chicago, IL **Sarasota, FL **Ft. Myers, FL **Portland, ME If you or anyone you know might be interested in more information on any of these positions please contact me at relia1@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From madary <@t> verizon.net Fri Apr 15 09:06:15 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Fri Apr 15 09:04:58 2011 Subject: [Histonet] transferring tssue sections Message-ID: <39715563.2417160.1302876375349.JavaMail.root@vznit170060> If there is still the old diatex compund out there, great way to transfer sections but very time consuming, couple of days. From madary <@t> verizon.net Fri Apr 15 09:08:47 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Fri Apr 15 09:07:19 2011 Subject: [Histonet] I am looking for a job in Histology Message-ID: <1398185972.2417346.1302876528063.JavaMail.root@vznit170060> Wondering if there are any staffing agencies who could contact me abo ut work in our great field. I live in Maryland and can work in the DC for Ju in Histol Nick(Rocky) Madary, HT/HTL(ASCP)QIHC Joni Madary, PhD(in life) Apr 14, 2011 12:00:16 PM, histonet@lists.utsouthwestern.edu wrote: Send Histonet histonet@lists.utsouthwestern.edu To http://lists.uts or, via email, send a message histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lis When replying, please edit your Subject line s specific than "Re: Contents of Histonet digest..." < 1. RE: Activated Carbon (Rathborne, Toni) ------------------------------------------------------------------- ---< Message: 1 Date: Thu, 14 Apr 2011 12:10:54 -0400 From: "Rathbo Subject: RE: [Histonet] To: "Travis Troyer" , Message-ID: Content-Type: text/ Which processor? -----Original Messa From: histonet-bounces@lists.utsouthwestern.edu [mailto:histo Travis Troyer Sent: To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Activated Carbon Does anyone have an ine the processor? Thanks, T _______________________________________________ Histonet mailin Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern. CONFIDENTIALITY NOTICE This mes Medical Center and a contained in this me propriet protection and/or exempt forwarding, print strictly prohi please promptly error by e-ma Desk at 908-6 Be sure to visit Somerset Medical Center's Web s www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. -------------- _______________________________________________ Histonet@lists.utsouthwestern.edu http://lists. End of Histonet Digest, **************************************** From laurie.colbert <@t> huntingtonhospital.com Fri Apr 15 09:12:11 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Apr 15 09:12:13 2011 Subject: [Histonet] ThermoScientific Printmate In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBBAEFA7D@IBMB7Exchange.digestivespecialists.com> References: <661949901A768E4F9CC16D8AF8F2838C03974BBD@is-e2k3.grhs.net> <5A2BD13465E061429D6455C8D6B40E390EBBAEFA7D@IBMB7Exchange.digestivespecialists.com> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AB3E@EXCHANGE3.huntingtonhospital.com> I really love our Printmate, but we have also had a fair amount of problems with it. We haven't really had issues with the cassettes jamming, but we've had the print head replaced numerous times. Thermo Fisher is aware of this widespread problem and has switched manufacturers for the print head. We have our Printmate set up to be able to scan various barcodes for the color of cassette (which determines which hopper the cassette will come from), the starting block number, and the total number of cassettes that we want printed for that case. It is very easy to use, and despite all of the problems that we've had, I think it is a great benefit to us and I would recommend it. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, April 15, 2011 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ThermoScientific Printmate So, is there a cassette printer out there that someone really loves? Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, April 15, 2011 9:28 AM To: Robin Negron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ThermoScientific Printmate I have had a Printmate for about 3 months now. I think the idea behind the design and how it is suppose to work is fine, but ours has not work correctly since we got it. It continues to jam up and we have had it worked on 3 times now. I think they have decided to replace the unit at this point (most likely with another unit that was sent in for repair and refurbished)! I see little difference in cost to run. We switched cassette vendors and they matched our current price. I think it is worth the added security of patient safety if I can ever get a unit that works. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Negron Sent: Thursday, April 14, 2011 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ThermoScientific Printmate Can anyone give me any information Re: Thermo Scientific Printmate? I would like to get general information about this equipment including maintenance experience, dependability, efficiency, and any other experiences you have had either liked or dislike about this machine. Thanks, Robin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Fri Apr 15 09:18:41 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Apr 15 09:18:41 2011 Subject: [Histonet] mold spray In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2AB3E@EXCHANGE3.huntingtonhospital.com> References: <661949901A768E4F9CC16D8AF8F2838C03974BBD@is-e2k3.grhs.net><5A2BD13465E061429D6455C8D6B40E390EBBAEFA7D@IBMB7Exchange.digestivespecialists.com> <57BE698966D5C54EAE8612E8941D76830AD2AB3E@EXCHANGE3.huntingtonhospital.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B370407ECC8@giamail2.Gia.com> Does anyone use any type of spray to keep the blocks from sticking to the molds when popping the blocks out after they've been embedded? From victor <@t> pathology.washington.edu Fri Apr 15 09:20:08 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Apr 15 09:20:22 2011 Subject: [Histonet] ThermoScientific Printmate In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBBAEFA7D@IBMB7Exchange.digestivespecialists.com> References: <661949901A768E4F9CC16D8AF8F2838C03974BBD@is-e2k3.grhs.net> <5A2BD13465E061429D6455C8D6B40E390EBBAEFA7D@IBMB7Exchange.digestivespecialists.com> Message-ID: <4DA85418.1080809@pathology.washington.edu> My apologies to the group and Thermo for my comment yesterday, if they seemed misleading. I was referencing the Slidemate not the Printmate. I have no personal knowledge of the Printmate. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 4/15/2011 6:42 AM, Blazek, Linda wrote: > So, is there a cassette printer out there that someone really loves? > Linda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Friday, April 15, 2011 9:28 AM > To: Robin Negron; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ThermoScientific Printmate > > I have had a Printmate for about 3 months now. I think the idea behind > the design and how it is suppose to work is fine, but ours has not work > correctly since we got it. It continues to jam up and we have had it > worked on 3 times now. I think they have decided to replace the unit at > this point (most likely with another unit that was sent in for repair > and refurbished)! I see little difference in cost to run. We switched > cassette vendors and they matched our current price. I think it is worth > the added security of patient safety if I can ever get a unit that > works. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin > Negron > Sent: Thursday, April 14, 2011 2:59 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ThermoScientific Printmate > > > > > Can anyone give me any information Re: Thermo Scientific > Printmate? > > > > I would like to get general information about this > equipment including maintenance experience, dependability, efficiency, > and any other experiences you have had either liked or dislike about > this machine. > > > > Thanks, > > > > Robin > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Apr 15 09:23:55 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Apr 15 09:24:12 2011 Subject: [Histonet] RE: mold spray In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370407ECC8@giamail2.Gia.com> References: <661949901A768E4F9CC16D8AF8F2838C03974BBD@is-e2k3.grhs.net><5A2BD13465E061429D6455C8D6B40E390EBBAEFA7D@IBMB7Exchange.digestivespecialists.com> <57BE698966D5C54EAE8612E8941D76830AD2AB3E@EXCHANGE3.huntingtonhospital.com> <03C921A1EAF7F541B16543F6EC6A4B370407ECC8@giamail2.Gia.com> Message-ID: Mercedes Medical has a good product: http://www.mercedesmedical.com/Default.aspx?page=category%20search%20results&CatList=31&Parent=44&tree=2*Histology+Supplies*0@@41*Cassettes%2c+Molds+and+Sectioning+Aids*0@@44*Base+Mold+Release*31@@ Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, April 15, 2011 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mold spray Does anyone use any type of spray to keep the blocks from sticking to the molds when popping the blocks out after they've been embedded? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Apr 15 09:25:15 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Apr 15 09:25:43 2011 Subject: [Histonet] mold spray In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370407ECC8@giamail2.Gia.com> Message-ID: We don't use a spray, but use Tissue-Tek Mold Release (Sakura) to clean them. http://www.sakuraeu.com/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Amber McKenzie Sent: Friday, April 15, 2011 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mold spray Does anyone use any type of spray to keep the blocks from sticking to the molds when popping the blocks out after they've been embedded? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From rjbuesa <@t> yahoo.com Fri Apr 15 09:31:00 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 15 09:31:08 2011 Subject: [Histonet] mold spray In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370407ECC8@giamail2.Gia.com> Message-ID: <973268.59454.qm@web65707.mail.ac4.yahoo.com> Prepare a mixture of isopropanol and mineral oil 20:1, shake well and spray on the molds before starting embedding. Ren? J. --- On Fri, 4/15/11, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] mold spray To: histonet@lists.utsouthwestern.edu Date: Friday, April 15, 2011, 10:18 AM Does anyone use any type of spray to keep the blocks from sticking to the molds when popping the blocks out after they've been embedded? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Fri Apr 15 12:34:12 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Apr 15 12:34:22 2011 Subject: [Histonet] mold spray In-Reply-To: <973268.59454.qm@web65707.mail.ac4.yahoo.com> References: <03C921A1EAF7F541B16543F6EC6A4B370407ECC8@giamail2.Gia.com> <973268.59454.qm@web65707.mail.ac4.yahoo.com> Message-ID: This works well too: Mold Release Expires 1 year Reagent alcohol 570 mL dH2O 30 mL Glycerol 35 mL On Fri, Apr 15, 2011 at 7:31 AM, Rene J Buesa wrote: > Prepare a mixture of isopropanol and mineral oil 20:1, shake well and spray on the molds before starting embedding. > Ren? J. > > --- On Fri, 4/15/11, Amber McKenzie wrote: > > > From: Amber McKenzie > Subject: [Histonet] mold spray > To: histonet@lists.utsouthwestern.edu > Date: Friday, April 15, 2011, 10:18 AM > > > Does anyone use any type of spray to keep the blocks from sticking to > the molds when popping the blocks out after they've been embedded? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From ddittus787 <@t> aol.com Fri Apr 15 12:37:01 2011 From: ddittus787 <@t> aol.com (ddittus787@aol.com) Date: Fri Apr 15 12:37:47 2011 Subject: [Histonet] mold spray In-Reply-To: References: <03C921A1EAF7F541B16543F6EC6A4B370407ECC8@giamail2.Gia.com><973268.59454.qm@web65707.mail.ac4.yahoo.com> Message-ID: <8CDC9AADCE1A28A-2044-24C68@webmail-m145.sysops.aol.com> I have also used PAM spray -----Original Message----- From: Patrick Laurie To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Sent: Fri, Apr 15, 2011 1:34 pm Subject: Re: [Histonet] mold spray This works well too: old Release Expires 1 year Reagent alcohol 570 mL dH2O 30 mL Glycerol 35 mL n Fri, Apr 15, 2011 at 7:31 AM, Rene J Buesa wrote: Prepare a mixture of isopropanol and mineral oil 20:1, shake well and spray on he molds before starting embedding. Ren? J. --- On Fri, 4/15/11, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] mold spray To: histonet@lists.utsouthwestern.edu Date: Friday, April 15, 2011, 10:18 AM Does anyone use any type of spray to keep the blocks from sticking to the molds when popping the blocks out after they've been embedded? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- atrick Laurie HT(ASCP)QIHC ellNetix Pathology & Laboratories 124 Columbia Street, Suite 200 eattle, WA 98104 laurie@cellnetix.com _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet = From katelin09htl <@t> gmail.com Fri Apr 15 13:26:08 2011 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Fri Apr 15 13:26:11 2011 Subject: [Histonet] Histology Supervisor Needed Message-ID: *Histology Supervisor * Full-time position is currently available for an enthusiastic BC/BE HT or HTL. This is a private histology laboratory for a growing pathology practice in the Greater Portland, Oregon area. The successful applicant will be responsible for the supervision of the histology laboratory including: oversight of lab assistants, accurate and timely preparation of material; routine stains, special stains, IHC, instrument/laboratory maintenance, and light clerical duties. Preference will be given to candidates with IHC experience. Please fax resume to (503) 670-9754 or email to katelin@medsurgpath.com Salary DOE -- Katelin Lester, HTL MedSurg Pathology Associates, Inc. (503) 443-2157 From mpence <@t> grhs.net Fri Apr 15 13:29:56 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Apr 15 13:30:00 2011 Subject: [Histonet] RE: I saw your post on HistoNet In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974BBE@is-e2k3.grhs.net> Why would I want to buy a new unit when I haven't had the one I have for working yet. This is why I hate to have vendors on this lis. They are like leaches waiting for a chance to reply off line and push their products good or bad! And they can't even get your name right. It's FRIDAY!!!! -----Original Message----- From: VanTilburg, Walt [mailto:WVanTilburg@GENERAL-DATA.com] Sent: Friday, April 15, 2011 1:07 PM To: Mike Pence Subject: I saw your post on HistoNet Mark, General Data Healthcare makes a superior cassette printer. I have attached ebrochures. If your lab is interested please contact me. Walt Walter Van Tilburg Director of Sales General Data Healthcare, Inc. 26500 Bruce Road Bay Village, Ohio 44140 440-823-5495 - Mobile 440-808-8983 - Office 440-808-8995 - Fax WVanTilburg@general-data.com Visit our web site http://www.general-data.com/healthcare/ ________________________________ This email may contain confidential General Data Company, Inc. information: any unauthorized or improper disclosure, copying, distribution or use of the contents of this email and attached document(s) is prohibited and may be a criminal offense. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you received this email in error, please reply immediately to the sender & delete this message and the attached document(s) without disclosure. From stephaniem <@t> wpcreno.com Fri Apr 15 13:39:39 2011 From: stephaniem <@t> wpcreno.com (Stephanie Myer) Date: Fri Apr 15 13:37:04 2011 Subject: [Histonet] SOX10 Antibody Message-ID: <914FDE201204274CB2B5808918E92474458A42@mailsvr.westernpathologyconsultants.com> Hello everyone, Is anybody currently using the SOX10 antibody for Immunohistochemistry? If so, what manufacturer are you using, please? This is my first time posting, and I thank you all for any input! Stephanie Myer, HT (ASCP)CM Laboratory Manager Nevada Histology, Inc. 1350 Stardust Street, Suite D Reno, NV 89503 775-747-2211 ph 775-747-2974 fax email: stephaniem@wpcreno.com www.westernpathology.com From sgoebel <@t> mirnarx.com Fri Apr 15 13:42:31 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Apr 15 13:42:36 2011 Subject: [Histonet] Cell Block IHC Message-ID: Hey ya'll, So I am trying to do IHC on paraffin embedded cell blocks. The cell blocks were made in an agar solution. The H&E slides came out fine and look like a normal cell block slide. However I am now a little confused. I put the slides through HIER and it doesn't look like there is anything on the slide anymore? Are the cells still there, just the agar fell off because of the heat? Should I continue to use all the reagents and antibody on these slides? Also, if they are trash...how do I do IHC stains on these blocks? I do not have access to a cytospin machine so my "traditional" way of doing ICC with cytospins I cannot do. Ahhh!! Help!!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From talulahgosh <@t> gmail.com Fri Apr 15 14:09:44 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Apr 15 14:09:48 2011 Subject: [Histonet] SOX10 Antibody In-Reply-To: <914FDE201204274CB2B5808918E92474458A42@mailsvr.westernpathologyconsultants.com> References: <914FDE201204274CB2B5808918E92474458A42@mailsvr.westernpathologyconsultants.com> Message-ID: We have tried rabbit anti SOX10 from Millipore and Santa Cruz. Neither were specific to chick tissue, even though they were supposed to be due to sequence homology. There's my absolutely not helpful at all contribution towards your question. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 15, 2011 at 2:39 PM, Stephanie Myer wrote: > Hello everyone, > > Is anybody currently using the SOX10 antibody for Immunohistochemistry? > If so, what manufacturer are you using, please? > > This is my first time posting, and I thank you all for any input! > > > Stephanie Myer, HT (ASCP)CM > Laboratory Manager > > Nevada Histology, Inc. > 1350 Stardust Street, Suite D > Reno, NV 89503 > 775-747-2211 ph > 775-747-2974 fax > email: stephaniem@wpcreno.com > www.westernpathology.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sgoebel <@t> mirnarx.com Fri Apr 15 14:23:40 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Apr 15 14:23:43 2011 Subject: [Histonet] Thank you all!! Message-ID: So...I sacrificed a couple of the slides and stained with H&E per ya'lls suggestion and...yay!! Cells all over the place. See I freaked myself out for nothing. Now, where is 5:00? =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From brian <@t> prometheushealthcare.com Fri Apr 15 14:29:47 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Fri Apr 15 14:29:59 2011 Subject: [Histonet] Histology and IHC Management Openings in New York Message-ID: <010e01cbfba3$7e8c3530$7ba49f90$@com> Growing reference lab in Westchester County, NY searching for the following openings: Manager, Lab Operations, Night, Histology between 9-10pm start, M-F Supervisor, IHC IHC 8am-4:30pm, T-S Manager, IHC IHC 8am-4:30pm, M-F They offer Medical Insurance, Dental Insurance, Vision Insurance, Employee Life and Accidental Death and Dismemberment Insurance, Short-Term and Long-Term Disability, 401(k) Retirement Savings Plan, Tuition Reimbursement, Paid Time Off including Holidays, Floating Holidays and Vacation, Sick Leave and Personal Time. Very Competitive salary!! If you might know anyone interested I would greatly appreciate it! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From deb.vaneyck <@t> phci.org Fri Apr 15 16:08:12 2011 From: deb.vaneyck <@t> phci.org (Van Eyck, Deb) Date: Fri Apr 15 16:08:48 2011 Subject: [Histonet] Histo gel users? In-Reply-To: <20110413140958.6F1317D0CB8@mx1.phci.org> References: <20110413140958.6F1317D0CB8@mx1.phci.org> Message-ID: <37212590E341574B96E6796D27DF42DA0194EEB2@RWDEX3.WHS.phci.org> For those of you that use Histogel to make cell blocks or with biopsy specimens - is it necessary to have your specimen completely fixed prior to making your pellet. Or does the NBF permeate the gel? What about using it on specimens that are going to be tested for prognostic markers? Like Her 2 neu? Thanks Deb. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, April 13, 2011 9:10 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 89, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Immunofluorescence and FISH Combined stating (Diane Kolins) 2. Von kossa stain (Webb, Dorothy L) 3. RE: Von kossa stain (Breeden, Sara) 4. Re: Histonet Digest, Vol 89, Issue 12 (Mark Elliott) 5. AW: [Histonet] Von kossa stain (Gudrun Lang) 6. Re: AW: [Histonet] Von kossa stain (Grantham, Andrea L - (algranth)) 7. Staining using a Plastic Sytem (Mahesh Polavarapu) 8. Biopsy program for Sakura VIP 5/6 Processor (Evans, Andria B) 9. Re: Milestone MW (Rene J Buesa) 10. Re: Von kossa stain (Rene J Buesa) 11. RE: Von kossa stain (Jack Ratliff) 12. RE: Counterstain for dual chromogenic (Tony Henwood) 13. RE: Von kossa stain (Tony Henwood) 14. RE: Biopsy program for Sakura VIP 5/6 Processor (Tony Henwood) 15. RE: RE: Biopsy program for Sakura VIP 5/6 Processor (WILLIAM DESALVO) 16. RE: RE: Biopsy program for Sakura VIP 5/6 Processor (Tony Henwood) 17. Update regarding question on Plastic Embedding (Mahesh Polavarapu) 18. SP3 HER2 mAb (Richard Cartun) 19. FW: 1 H&E slide vs. 2 (Eugenia Thomas) 20. Ventana ultra (Barbara.Crill@LPNT.net) 21. RE: FW: 1 H&E slide vs. 2 (Monfils, Paul) 22. RE: Ventana ultra (Sebree Linda A) ---------------------------------------------------------------------- Message: 1 Date: Tue, 12 Apr 2011 13:23:03 -0400 From: Diane Kolins Subject: [Histonet] Immunofluorescence and FISH Combined stating To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Where can I find a procedure for dual staining of blood and bone marrow smears using Vector anti-kappa and anti-lambda tagged with Coumarine followed by FISH hybridization. I am able to see what I think are plasma cells by their fluorescent blue cytoplasm and on the same slide (changing filters) I can see the red and green signal patterns. From: : diane@bioview.co.il ------------------------------ Message: 2 Date: Tue, 12 Apr 2011 12:35:18 -0500 From: "Webb, Dorothy L" Subject: [Histonet] Von kossa stain To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 3 Date: Tue, 12 Apr 2011 11:52:49 -0600 From: "Breeden, Sara" Subject: RE: [Histonet] Von kossa stain To: "Webb, Dorothy L" , Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E477BD@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Funny you should ask... just last week I had two requests for a Von Kossa. That's when I found out that, despite the fact that we just moved into a brand new building with all the bells and whistles, there was not one single UV light in any of the many hoods that had been installed. Heaven forbid I could even find an incandescent bulb (we are a "green building"). So, I prepped my slides, put them in a clear glass Coplin jar and parked the jar on the hood of my car for an hour. Works like a charm. This all depends, naturally, on (1) if you even HAVE sunshine where you live; (2) how far a walk it is to the sunshine, and (3) whether you have a car hood on which to park the Coplin jar. Needless to say, out of the line of sight of curious onlookers with sticky fingers and no business wondering what that glass jar is... Nonetheless, it works just fine! May the Force be with you. ------------------------------ Message: 4 Date: Tue, 12 Apr 2011 11:16:16 -0700 From: "Mark Elliott" Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 12 To: Message-ID: <4DA43480020000D60006111F@mail.mrl.ubc.ca> Content-Type: text/plain; charset=US-ASCII Date: Tue, 12 Apr 2011 11:37:17 -0400 From: Eva Permaul Subject: [Histonet] Counterstain for dual chromogenic To: histonet@lists.utsouthwestern.edu Message-ID: <4DA471AD.3040002@georgetown.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Eva I have been doing some double labelling using Vulcan Fast Red from Biocare and Vector Blue Alkaline Phosphatase Substrate Kit III from Vector Labs. For counter stain I use Vector Methyl Green and the combination works great and looks quite nice. Mark Good morning, I have been attempting to do some dual chromogenic staining. I am using a mouse and a rabbit antibody. For the mouse antibody I am using an HRP-mouse secondary followed by AEC for visualization. For the rabbit antibody I am using a biotinylated rabbit secondary, followed by ABC-AP and vector blue. My problem is what to use as a counterstain. From everything I have read so far there isn't one that would work without having some problems. I was thinking of trying a very light Hematoxylin and see if it doesn't disrupt the vector blue visualization too much. Does anyone else have any suggestions? Thanks, Eva ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ------------------------------ Message: 5 Date: Tue, 12 Apr 2011 20:22:22 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Von kossa stain To: "'Webb, Dorothy L'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <13B705C625E74F1BB9337593D57C3658@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" WE use just a simple desk-lamp and put the coplin jar directly under the bulb. And the desk-lamp is placed in the window. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, Dorothy L Gesendet: Dienstag, 12. April 2011 19:35 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Von kossa stain What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 12 Apr 2011 11:35:47 -0700 From: "Grantham, Andrea L - (algranth)" Subject: Re: AW: [Histonet] Von kossa stain Cc: HISTONET Message-ID: <59222C2B-42C1-4EB6-8233-ED0FB7FCA354@email.arizona.edu> Content-Type: text/plain; charset="iso-8859-1" We have intense sun here but my windows face the wrong way! At least I have windows. I just use a light bulb from the lamp we have over the coverslipping station. Works great. Andi On Apr 12, 2011, at 11:22 AM, Gudrun Lang wrote: > WE use just a simple desk-lamp and put the coplin jar directly under the > bulb. And the desk-lamp is placed in the window. > > Bye Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, > Dorothy L > Gesendet: Dienstag, 12. April 2011 19:35 > An: 'histonet@lists.utsouthwestern.edu' > Betreff: [Histonet] Von kossa stain > > What is everyone using for their "light" when developing the silver in the > VonKossa stain when you have no sunlight to use? We used to use a 60 watt > lamp, but haven't done one for years and am bringing this stain back to our > repetiore due to pathologist request. Thanks much! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Tue, 12 Apr 2011 13:56:53 -0500 From: Mahesh Polavarapu Subject: [Histonet] Staining using a Plastic Sytem To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, Does anyone have experience embedding and staining in plastic? We have rabbit rotator cuff tendons that are being embedded in plastic. I am trying to figure out what the process is and which stains to use in order to visualize (and quantify) blood vessels and collagen deposition at the Bone (Humeral Head) - Tendon (Infraspinatus) interface. Thanks, Mahesh ------------------------------ Message: 8 Date: Tue, 12 Apr 2011 14:57:18 -0400 From: "Evans, Andria B" Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor To: "histonet@lists.utsouthwestern.edu" Message-ID: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60@MAIL-AG-CLUSTER.lha.org> Content-Type: text/plain; charset="iso-8859-1" Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is.... Formalin 1 min 37degrees Formalin 15 mins 37degrees 70 14 mins 40 degrees 95 14 mins 40 degrees 95 9 mins 40 degrees 100 9 mins 40 degrees 100 7 mins 40 degrees 100 4 mins 40 degrees Xylene 23 mins no heat Xylene 15 mins no heat Paraffin 20 mins 60 degrees Paraffin 18 mins 60 degrees Paraffin 10 mins 60 degrees Paraffin 0 mins 60 degrees All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges. Any feedback would be greatly appreciated. Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 aevans3@lgheath.org This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Tue, 12 Apr 2011 12:27:49 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Milestone MW To: Histonet@lists.utsouthwestern.edu, SHANE NELSON Message-ID: <809117.78546.qm@web65715.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Model "KOS" by Milestone is capable of tissue processing, decalcification, special stains, fixation, gross hardening, and antigen retrieval. Ren? J. --- On Tue, 4/12/11, SHANE NELSON wrote: From: SHANE NELSON Subject: [Histonet] Milestone MW To: Histonet@lists.utsouthwestern.edu Date: Tuesday, April 12, 2011, 12:30 PM Margret, Which microwave would that be ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS 35-900 Bob Hope Drive Suite 275 Rancho Mirage, Ca.? 92270 cell (909) 841-9761 / wk (760) 321-2500 nelsonrnch@verizon.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 12 Apr 2011 12:30:26 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Von kossa stain To: "'histonet@lists.utsouthwestern.edu'" , Dorothy LWebb Message-ID: <145774.98333.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You can use any strong intensity microscope light. The stronger the?intensity of the light the better, but the reduction has a cumulative effect so even not very strong intensity bulbs can be effective. Ren? J. --- On Tue, 4/12/11, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Von kossa stain To: "'histonet@lists.utsouthwestern.edu'" Date: Tuesday, April 12, 2011, 1:35 PM What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use?? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request.? Thanks much! Dorothy Webb, HT (ASCP) Regions Histology Technical Supervisor 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 12 Apr 2011 15:41:54 -0400 From: Jack Ratliff Subject: RE: [Histonet] Von kossa stain To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I purchase a kit from Dorn and Hart Microedge and use sodium carbonate-formaldehyde solution to chemically develop. Stain in silver nitrate for 5 min in the dark, 3 fresh DI rinses for 1 min each in the dark, then sodium carbonate-formaldehyde solution for 2 min in the dark, 2 fresh DI rinses (normal light) for 1 min each, 30 seconds in Farmer's Diminisher (Sodium thiosulfate-potassium ferricyanide soluiton to stop reaction) and a running tap water rinse for 10 min. Jack > From: Dorothy.L.Webb@HealthPartners.Com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 12:35:18 -0500 > Subject: [Histonet] Von kossa stain > > What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 12 Apr 2011 23:11:35 +0000 From: Tony Henwood Subject: RE: [Histonet] Counterstain for dual chromogenic To: "'Eva Permaul'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E925@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" Try ethyl green: 1. 0.1N Acetic Acid Add 6ml of glacial acetic acid to 1000 ml of distilled water. 2. 0.1N Sodium Acetate Add 4.102 g of sodium acetate to 500 ml of distilled water 3. pH 4.2 buffer Add 755 ml of 0.1N acetic acid to 264 ml of 0.1N Sodium Acetate and adjust the pH to 4.2 with 1M NaOH 4. Ethyl Green Solution To 100ml of buffer add 2g Ethyl Green (CI 42590) Can be stored at room temp. If staining turns bluish than the solution has started to deteriorate. Stain for 5 minutes. Do not rinse in water for too long, it tends to extract the green staining Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Wednesday, 13 April 2011 1:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counterstain for dual chromogenic Good morning, I have been attempting to do some dual chromogenic staining. I am using a mouse and a rabbit antibody. For the mouse antibody I am using an HRP-mouse secondary followed by AEC for visualization. For the rabbit antibody I am using a biotinylated rabbit secondary, followed by ABC-AP and vector blue. My problem is what to use as a counterstain. From everything I have read so far there isn't one that would work without having some problems. I was thinking of trying a very light Hematoxylin and see if it doesn't disrupt the vector blue visualization too much. Does anyone else have any suggestions? Thanks, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 13 Date: Tue, 12 Apr 2011 23:22:21 +0000 From: Tony Henwood Subject: RE: [Histonet] Von kossa stain To: "'Breeden, Sara'" , "Webb, Dorothy L" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E95A@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" Interesting, Meloan & Puchtler (1985) have drawn our attention to Von Kossa's original work on this technique. He regarded only the yellow colouration of calcium deposits during early stages of the reaction as diagnostic for calcium phosphate and credited the blackening to organic matter. Further studies showed that bright light only causes the irreversible blackening of organic matter that masks the yellow silver phosphate. When the reaction is performed in subdued light, yellow to yellowish brown silver phosphate is visualised selectively. Silver carbonate dissolves in sodium thiosulphate and cannot be demonstrated with von Kossa's technique (Meloan & Puchtler 1985 J Histotechnol 8(1):11-13.). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, 13 April 2011 3:53 AM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Von kossa stain Funny you should ask... just last week I had two requests for a Von Kossa. That's when I found out that, despite the fact that we just moved into a brand new building with all the bells and whistles, there was not one single UV light in any of the many hoods that had been installed. Heaven forbid I could even find an incandescent bulb (we are a "green building"). So, I prepped my slides, put them in a clear glass Coplin jar and parked the jar on the hood of my car for an hour. Works like a charm. This all depends, naturally, on (1) if you even HAVE sunshine where you live; (2) how far a walk it is to the sunshine, and (3) whether you have a car hood on which to park the Coplin jar. Needless to say, out of the line of sight of curious onlookers with sticky fingers and no business wondering what that glass jar is... Nonetheless, it works just fine! May the Force be with you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 14 Date: Tue, 12 Apr 2011 23:30:30 +0000 From: Tony Henwood Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor To: "'Evans, Andria B'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E981@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Wednesday, 13 April 2011 4:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is.... Formalin 1 min 37degrees Formalin 15 mins 37degrees 70 14 mins 40 degrees 95 14 mins 40 degrees 95 9 mins 40 degrees 100 9 mins 40 degrees 100 7 mins 40 degrees 100 4 mins 40 degrees Xylene 23 mins no heat Xylene 15 mins no heat Paraffin 20 mins 60 degrees Paraffin 18 mins 60 degrees Paraffin 10 mins 60 degrees Paraffin 0 mins 60 degrees All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges. Any feedback would be greatly appreciated. Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 aevans3@lgheath.org This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 15 Date: Tue, 12 Apr 2011 17:58:36 -0600 From: WILLIAM DESALVO Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor To: , , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I would consider replacing the sponges. Tony is correct that you need to make sure the air is removed from the sponge to facilitate exchange, but with shortened processing times you will undoubtedly cause carry over from solution to solution. Yuo may want to have a small container of alcoholic formalin with sponges sitting on the gross table. Try using a nylon tissue bag to funnel filter your small biopsies. all your biopsy sample sizes can be placed into the bag and very tiny samples are retained. Last suggestion is to consider a folded filter paper method (we name it origami, I can provide directly if you want) that uses a quick four fold process w/ forceps at the gross bench to create a pocket for the tiny samples, has a single layer between the tissue/solution and is easy to open at embedding (without "popping" of tissue samples). Avoind the unnecessary carryover of the sponge if you can. William DeSalvo, B.S., HTL(ASCP) > From: AnthonyH@chw.edu.au > To: aevans3@lghealth.org; histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 23:30:30 +0000 > CC: > Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor > > Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick). > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B > Sent: Wednesday, 13 April 2011 4:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor > > Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is.... > > Formalin 1 min 37degrees > Formalin 15 mins 37degrees > 70 14 mins 40 degrees > 95 14 mins 40 degrees > 95 9 mins 40 degrees > 100 9 mins 40 degrees > 100 7 mins 40 degrees > 100 4 mins 40 degrees > Xylene 23 mins no heat > Xylene 15 mins no heat > Paraffin 20 mins 60 degrees > Paraffin 18 mins 60 degrees > Paraffin 10 mins 60 degrees > Paraffin 0 mins 60 degrees > > All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges. > > Any feedback would be greatly appreciated. > > Andria B Evans HTL(ASCP)CM > Lancaster General Hospital > 555 North Duke Street > Lancaster, PA 17604 > (717)544-5511 ext: 77329 > aevans3@lgheath.org > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. > Any unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 13 Apr 2011 00:10:04 +0000 From: Tony Henwood Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor To: "'WILLIAM DESALVO'" , "aevans3@lghealth.org" , histonet Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E9D3@xmdb02.nch.kids> Content-Type: text/plain; charset="us-ascii" I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Wednesday, 13 April 2011 9:59 AM To: Tony Henwood; aevans3@lghealth.org; histonet Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor I would consider replacing the sponges. Tony is correct that you need to make sure the air is removed from the sponge to facilitate exchange, but with shortened processing times you will undoubtedly cause carry over from solution to solution. Yuo may want to have a small container of alcoholic formalin with sponges sitting on the gross table. Try using a nylon tissue bag to funnel filter your small biopsies. all your biopsy sample sizes can be placed into the bag and very tiny samples are retained. Last suggestion is to consider a folded filter paper method (we name it origami, I can provide directly if you want) that uses a quick four fold process w/ forceps at the gross bench to create a pocket for the tiny samples, has a single layer between the tissue/solution and is easy to open at embedding (without "popping" of tissue samples). Avoind the unnecessary carryover of the sponge if you can. William DeSalvo, B.S., HTL(ASCP) > From: AnthonyH@chw.edu.au > To: aevans3@lghealth.org; histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 23:30:30 +0000 > CC: > Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor > > Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick). > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B > Sent: Wednesday, 13 April 2011 4:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor > > Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is.... > > Formalin 1 min 37degrees > Formalin 15 mins 37degrees > 70 14 mins 40 degrees > 95 14 mins 40 degrees > 95 9 mins 40 degrees > 100 9 mins 40 degrees > 100 7 mins 40 degrees > 100 4 mins 40 degrees > Xylene 23 mins no heat > Xylene 15 mins no heat > Paraffin 20 mins 60 degrees > Paraffin 18 mins 60 degrees > Paraffin 10 mins 60 degrees > Paraffin 0 mins 60 degrees > > All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges. > > Any feedback would be greatly appreciated. > > Andria B Evans HTL(ASCP)CM > Lancaster General Hospital > 555 North Duke Street > Lancaster, PA 17604 > (717)544-5511 ext: 77329 > aevans3@lgheath.org> > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. > Any unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Tue, 12 Apr 2011 19:25:30 -0500 From: Mahesh Polavarapu Subject: [Histonet] Update regarding question on Plastic Embedding To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Looking for a protocol to visualize vascularization and collagen deposition at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic system. Sections will be rather thick (~50um) b/c they are being made through a titanium anchor. Using MMA with a cold-curing resin, Technovit 9100. Thanks in advance! - Mahesh ------------------------------ Message: 18 Date: Tue, 12 Apr 2011 20:34:51 -0400 From: "Richard Cartun" Subject: [Histonet] SP3 HER2 mAb To: "Histonet" Message-ID: <4DA4B76A.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII Anyone using the HER2 rabbit monoclonal antibody (clone SP3) ever see reactivity with skeletal muscle? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 19 Date: Tue, 12 Apr 2011 23:14:30 -0400 From: Eugenia Thomas Subject: [Histonet] FW: 1 H&E slide vs. 2 To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" From: eugenia902d1@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: 1 H&E slide vs. 2 Date: Mon, 11 Apr 2011 13:06:59 -0400 Good afternoon everyone, Does anyone know of an article or statistics discussing the impact (medical diagnosing) of cutting 2 H&E slides per block verses 1 for all routine work? Genia ------------------------------ Message: 20 Date: Wed, 13 Apr 2011 08:39:22 -0500 From: Subject: [Histonet] Ventana ultra To: Message-ID: <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net ------------------------------ Message: 21 Date: Wed, 13 Apr 2011 10:00:09 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] FW: 1 H&E slide vs. 2 To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E08020E55@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" When I was in clinical histology (I'm in research now) the pathologists didn't even want us putting two sections on a slide, because even though they knew a second serial section wasn't going to show anything the first section didn't show, they felt ethically/legally bound to examine both sections if they were there, so they were investing twice the time and effort for the same return. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eugenia Thomas Sent: Tuesday, April 12, 2011 11:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: 1 H&E slide vs. 2 From: eugenia902d1@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: 1 H&E slide vs. 2 Date: Mon, 11 Apr 2011 13:06:59 -0400 Good afternoon everyone, Does anyone know of an article or statistics discussing the impact (medical diagnosing) of cutting 2 H&E slides per block verses 1 for all routine work? Genia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 13 Apr 2011 09:09:22 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Ventana ultra To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Please "Reply All" as I'd like to hear feedback also. Thanks. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Wednesday, April 13, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 89, Issue 13 **************************************** This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. From morgancd <@t> charter.net Sat Apr 16 17:30:23 2011 From: morgancd <@t> charter.net (morgancd@charter.net) Date: Sat Apr 16 17:30:33 2011 Subject: [Histonet] Histo gel users? Message-ID: <34fd19ba.11a3ec.12f606fb3de.Webtop.43@charter.net> The specimen does not need to be fixed it will fix in the NBF during processing. Dawn F. Morgan > What about using it on specimens that are going to be tested for > prognostic markers? Like Her 2 neu? Thanks Deb. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: Wednesday, April 13, 2011 9:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 89, Issue 13 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Immunofluorescence and FISH Combined stating (Diane Kolins) > 2. Von kossa stain (Webb, Dorothy L) > 3. RE: Von kossa stain (Breeden, Sara) > 4. Re: Histonet Digest, Vol 89, Issue 12 (Mark Elliott) > 5. AW: [Histonet] Von kossa stain (Gudrun Lang) > 6. Re: AW: [Histonet] Von kossa stain > (Grantham, Andrea L - (algranth)) > 7. Staining using a Plastic Sytem (Mahesh Polavarapu) > 8. Biopsy program for Sakura VIP 5/6 Processor (Evans, Andria B) > 9. Re: Milestone MW (Rene J Buesa) > 10. Re: Von kossa stain (Rene J Buesa) > 11. RE: Von kossa stain (Jack Ratliff) > 12. RE: Counterstain for dual chromogenic (Tony Henwood) > 13. RE: Von kossa stain (Tony Henwood) > 14. RE: Biopsy program for Sakura VIP 5/6 Processor (Tony Henwood) > 15. RE: RE: Biopsy program for Sakura VIP 5/6 Processor > (WILLIAM DESALVO) > 16. RE: RE: Biopsy program for Sakura VIP 5/6 Processor (Tony > Henwood) > 17. Update regarding question on Plastic Embedding (Mahesh > Polavarapu) > 18. SP3 HER2 mAb (Richard Cartun) > 19. FW: 1 H&E slide vs. 2 (Eugenia Thomas) > 20. Ventana ultra (Barbara.Crill@LPNT.net) > 21. RE: FW: 1 H&E slide vs. 2 (Monfils, Paul) > 22. RE: Ventana ultra (Sebree Linda A) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 12 Apr 2011 13:23:03 -0400 > From: Diane Kolins > Subject: [Histonet] Immunofluorescence and FISH Combined stating > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > > Content-Type: text/plain; charset=us-ascii > > Where can I find a procedure for dual staining of blood and bone > marrow > smears using Vector anti-kappa and anti-lambda tagged with Coumarine > followed by FISH hybridization. I am able to see what I think are > plasma > cells by their fluorescent blue cytoplasm and on the same slide > (changing > filters) I can see the red and green signal patterns. > > > From: : diane@bioview.co.il > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 12 Apr 2011 12:35:18 -0500 > From: "Webb, Dorothy L" > Subject: [Histonet] Von kossa stain > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > <65365F35C0F2EF4D846EC3CA73E49C43010F851054C1@HPEMX3.HealthPartners.int> > > Content-Type: text/plain; charset="us-ascii" > > What is everyone using for their "light" when developing the silver in > the VonKossa stain when you have no sunlight to use? We used to use a > 60 watt lamp, but haven't done one for years and am bringing this > stain back to our repetiore due to pathologist request. Thanks much! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners R001.0 > > > ------------------------------ > > Message: 3 > Date: Tue, 12 Apr 2011 11:52:49 -0600 > From: "Breeden, Sara" > Subject: RE: [Histonet] Von kossa stain > To: "Webb, Dorothy L" , > > Message-ID: > > <4D14F0FC9316DD41972D5F03C070908B02E477BD@nmdamailsvr.nmda.ad.nmsu.edu> > > Content-Type: text/plain; charset="us-ascii" > > Funny you should ask... just last week I had two requests for a Von > Kossa. That's when I found out that, despite the fact that we just > moved into a brand new building with all the bells and whistles, there > was not one single UV light in any of the many hoods that had been > installed. Heaven forbid I could even find an incandescent bulb (we > are a "green building"). So, I prepped my slides, put them in a clear > glass Coplin jar and parked the jar on the hood of my car for an hour. > > Works like a charm. This all depends, naturally, on (1) if you even > HAVE sunshine where you live; (2) how far a walk it is to the > sunshine, > and (3) whether you have a car hood on which to park the Coplin jar. > Needless to say, out of the line of sight of curious onlookers with > sticky fingers and no business wondering what that glass jar is... > Nonetheless, it works just fine! May the Force be with you. > > > > ------------------------------ > > Message: 4 > Date: Tue, 12 Apr 2011 11:16:16 -0700 > From: "Mark Elliott" > Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 12 > To: > Message-ID: <4DA43480020000D60006111F@mail.mrl.ubc.ca> > Content-Type: text/plain; charset=US-ASCII > > > Date: Tue, 12 Apr 2011 11:37:17 -0400 > From: Eva Permaul > Subject: [Histonet] Counterstain for dual chromogenic > To: histonet@lists.utsouthwestern.edu > Message-ID: <4DA471AD.3040002@georgetown.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Eva > I have been doing some double labelling using Vulcan Fast Red from > Biocare and Vector Blue Alkaline Phosphatase Substrate Kit III from > Vector Labs. For counter stain I use Vector Methyl Green and the > combination works great and looks quite nice. > Mark > Good morning, > I have been attempting to do some dual chromogenic staining. I am > using a mouse and a rabbit antibody. For the mouse antibody I am using > an HRP-mouse secondary followed by AEC for visualization. For the > rabbit antibody I am using a biotinylated rabbit secondary, followed > by ABC-AP and vector blue. My problem is what to use as a > counterstain. From everything I have read so far there isn't one that > would work without having some problems. I was thinking of trying a > very light Hematoxylin and see if it doesn't disrupt the vector blue > visualization too much. Does anyone else have any suggestions? > Thanks, > Eva > > ***CONFIDENTIALITY NOTICE*** > This electronic message and any attachments are intended only for the > use of the addressee and may contain information that is privileged > and confidential. Any dissemination, distribution or copying of this > communication by unauthorized individuals is strictly prohibited. If > you have received this communication in error, please notify the > sender immediately by reply e-mail and delete the original and all > copies from your system. > > > > ------------------------------ > > Message: 5 > Date: Tue, 12 Apr 2011 20:22:22 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Von kossa stain > To: "'Webb, Dorothy L'" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <13B705C625E74F1BB9337593D57C3658@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > WE use just a simple desk-lamp and put the coplin jar directly under > the > bulb. And the desk-lamp is placed in the window. > > Bye Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > Webb, > Dorothy L > Gesendet: Dienstag, 12. April 2011 19:35 > An: 'histonet@lists.utsouthwestern.edu' > Betreff: [Histonet] Von kossa stain > > What is everyone using for their "light" when developing the silver in > the > VonKossa stain when you have no sunlight to use? We used to use a 60 > watt > lamp, but haven't done one for years and am bringing this stain back > to our > repetiore due to pathologist request. Thanks much! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is > strictly > prohibited. > > If you have received this e-mail in error, please immediately notify > the > HealthPartners Support Center by telephone at (952) 967-6600. You will > be > reimbursed for reasonable costs incurred in notifying us. > HealthPartners > R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Tue, 12 Apr 2011 11:35:47 -0700 > From: "Grantham, Andrea L - (algranth)" > Subject: Re: AW: [Histonet] Von kossa stain > Cc: HISTONET > Message-ID: <59222C2B-42C1-4EB6-8233-ED0FB7FCA354@email.arizona.edu> > Content-Type: text/plain; charset="iso-8859-1" > > We have intense sun here but my windows face the wrong way! At least I > have windows. I just use a light bulb from the lamp we have over the > coverslipping station. Works great. > > Andi > > > > On Apr 12, 2011, at 11:22 AM, Gudrun Lang wrote: > >> WE use just a simple desk-lamp and put the coplin jar directly under >> the >> bulb. And the desk-lamp is placed in the window. >> >> Bye Gudrun >> >> -----Urspr?ngliche Nachricht----- >> Von: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von >> Webb, >> Dorothy L >> Gesendet: Dienstag, 12. April 2011 19:35 >> An: 'histonet@lists.utsouthwestern.edu' >> Betreff: [Histonet] Von kossa stain >> >> What is everyone using for their "light" when developing the silver >> in the >> VonKossa stain when you have no sunlight to use? We used to use a 60 >> watt >> lamp, but haven't done one for years and am bringing this stain back >> to our >> repetiore due to pathologist request. Thanks much! >> >> Dorothy Webb, HT (ASCP) >> Regions Histology Technical Supervisor >> 651-254-2962 >> >> >> >> >> ________________________________ >> This e-mail and any files transmitted with it are confidential and >> are >> intended solely for the use of the individual or entity to whom they >> are >> addressed. If you are not the intended recipient or the individual >> responsible for delivering the e-mail to the intended recipient, >> please be >> advised that you have received this e-mail in error and that any use, >> dissemination, forwarding, printing, or copying of this e-mail is >> strictly >> prohibited. >> >> If you have received this e-mail in error, please immediately notify >> the >> HealthPartners Support Center by telephone at (952) 967-6600. You >> will be >> reimbursed for reasonable costs incurred in notifying us. >> HealthPartners >> R001.0 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > ------------------------------ > > Message: 7 > Date: Tue, 12 Apr 2011 13:56:53 -0500 > From: Mahesh Polavarapu > Subject: [Histonet] Staining using a Plastic Sytem > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > > Does anyone have experience embedding and staining in plastic? We have > rabbit rotator cuff tendons that are being embedded in plastic. I am > trying > to figure out what the process is and which stains to use in order to > visualize (and quantify) blood vessels and collagen deposition at the > Bone > (Humeral Head) - Tendon (Infraspinatus) interface. > > Thanks, > Mahesh > > > ------------------------------ > > Message: 8 > Date: Tue, 12 Apr 2011 14:57:18 -0400 > From: "Evans, Andria B" > Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60@MAIL-AG-CLUSTER.lha.org> > Content-Type: text/plain; charset="iso-8859-1" > > Our lab is currently looking for a way to shorten our Biopsy > processing program without compromising the patient specimen. We do > have an issue with our GI's being very dry, which causes us to have to > soak between each level taken and also causes a lot of chatter. Also > we have a goal to do a run during the day to improve turn around time. > Here is what our current protocol is.... > > Formalin 1 min 37degrees > Formalin 15 mins 37degrees > 70 14 mins 40 degrees > 95 14 mins 40 degrees > 95 9 mins 40 degrees > 100 9 mins 40 degrees > 100 7 mins 40 degrees > 100 4 mins 40 degrees > Xylene 23 mins no heat > Xylene 15 mins no heat > Paraffin 20 mins 60 degrees > Paraffin 18 mins 60 degrees > Paraffin 10 mins 60 degrees > Paraffin 0 mins 60 degrees > > All the steps are set on a fast mix setting. All of our biopsy > specimens are put into sponges. > > Any feedback would be greatly appreciated. > > Andria B Evans HTL(ASCP)CM > Lancaster General Hospital > 555 North Duke Street > Lancaster, PA 17604 > (717)544-5511 ext: 77329 > aevans3@lgheath.org > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use > of intended recipient(s) and may contain confidential and > privileged information. Any unauthorized review, use, disclosure or > distribution is > prohibited. If you are not the intended recipient, please contact the > sender by > reply e-mail and destroy all copies of the original message. > > > > ------------------------------ > > Message: 9 > Date: Tue, 12 Apr 2011 12:27:49 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Milestone MW > To: Histonet@lists.utsouthwestern.edu, SHANE NELSON > > Message-ID: <809117.78546.qm@web65715.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Model "KOS" by Milestone is capable of tissue processing, > decalcification, special stains, fixation, gross hardening, and > antigen retrieval. > Ren? J. > > --- On Tue, 4/12/11, SHANE NELSON wrote: > > > From: SHANE NELSON > Subject: [Histonet] Milestone MW > To: Histonet@lists.utsouthwestern.edu > Date: Tuesday, April 12, 2011, 12:30 PM > > > Margret, > Which microwave would that be > ? > THANK YOU, > ? > PATTI RUBEN-NELSON? H.T.(ASCP) LABORATORY CONSULTANT > SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS > 35-900 Bob Hope Drive > Suite 275 > Rancho Mirage, Ca.? 92270 > cell (909) 841-9761 / wk (760) 321-2500 > nelsonrnch@verizon.net > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Tue, 12 Apr 2011 12:30:26 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Von kossa stain > To: "'histonet@lists.utsouthwestern.edu'" > , Dorothy LWebb > > Message-ID: <145774.98333.qm@web65704.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > You can use any strong intensity microscope light. The stronger > the?intensity of the light the better, but the reduction has a > cumulative effect so even not very strong intensity bulbs can be > effective. > Ren? J. > > --- On Tue, 4/12/11, Webb, Dorothy L > wrote: > > > From: Webb, Dorothy L > Subject: [Histonet] Von kossa stain > To: "'histonet@lists.utsouthwestern.edu'" > > Date: Tuesday, April 12, 2011, 1:35 PM > > > What is everyone using for their "light" when developing the silver in > the VonKossa stain when you have no sunlight to use?? We used to use a > 60 watt lamp, but haven't done one for years and am bringing this > stain back to our repetiore due to pathologist request.? Thanks much! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > > ? ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 11 > Date: Tue, 12 Apr 2011 15:41:54 -0400 > From: Jack Ratliff > Subject: RE: [Histonet] Von kossa stain > To: , Histonet > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > I purchase a kit from Dorn and Hart Microedge and use sodium > carbonate-formaldehyde solution to chemically develop. > > Stain in silver nitrate for 5 min in the dark, 3 fresh DI rinses for 1 > min each in the dark, then sodium carbonate-formaldehyde solution for > 2 min in the dark, 2 fresh DI rinses (normal light) for 1 min each, 30 > seconds in Farmer's Diminisher (Sodium thiosulfate-potassium > ferricyanide soluiton to stop reaction) and a running tap water rinse > for 10 min. > Jack > > >> From: Dorothy.L.Webb@HealthPartners.Com >> To: histonet@lists.utsouthwestern.edu >> Date: Tue, 12 Apr 2011 12:35:18 -0500 >> Subject: [Histonet] Von kossa stain >> >> What is everyone using for their "light" when developing the silver >> in the VonKossa stain when you have no sunlight to use? We used to >> use a 60 watt lamp, but haven't done one for years and am bringing >> this stain back to our repetiore due to pathologist request. Thanks >> much! >> >> Dorothy Webb, HT (ASCP) >> Regions Histology Technical Supervisor >> 651-254-2962 >> >> >> >> >> ________________________________ >> This e-mail and any files transmitted with it are confidential and >> are intended solely for the use of the individual or entity to whom >> they are addressed. If you are not the intended recipient or the >> individual responsible for delivering the e-mail to the intended >> recipient, please be advised that you have received this e-mail in >> error and that any use, dissemination, forwarding, printing, or >> copying of this e-mail is strictly prohibited. >> >> If you have received this e-mail in error, please immediately notify >> the HealthPartners Support Center by telephone at (952) 967-6600. You >> will be reimbursed for reasonable costs incurred in notifying us. >> HealthPartners R001.0 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 12 > Date: Tue, 12 Apr 2011 23:11:35 +0000 > From: Tony Henwood > Subject: RE: [Histonet] Counterstain for dual chromogenic > To: "'Eva Permaul'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E925@xmdb02.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Try ethyl green: > 1. 0.1N Acetic Acid > Add 6ml of glacial acetic acid to 1000 ml of distilled water. 2. > 0.1N Sodium Acetate > Add 4.102 g of sodium acetate to 500 ml of distilled water > 3. pH 4.2 buffer > Add 755 ml of 0.1N acetic acid to 264 ml of 0.1N Sodium Acetate and > adjust the pH to 4.2 with 1M NaOH > 4. Ethyl Green Solution > To 100ml of buffer add 2g Ethyl Green (CI 42590) > Can be stored at room temp. If staining turns bluish than the > solution has started to deteriorate. > > Stain for 5 minutes. Do not rinse in water for too long, it tends to > extract the green staining > > Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), > FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 > Fax: 612 9845 3318 the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva > Permaul > Sent: Wednesday, 13 April 2011 1:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Counterstain for dual chromogenic > > Good morning, > I have been attempting to do some dual chromogenic staining. I am > using a mouse and a rabbit antibody. For the mouse antibody I am using > an HRP-mouse secondary followed by AEC for visualization. For the > rabbit antibody I am using a biotinylated rabbit secondary, followed > by ABC-AP and vector blue. My problem is what to use as a > counterstain. From everything I have read so far there isn't one that > would work without having some problems. I was thinking of trying a > very light Hematoxylin and see if it doesn't disrupt the vector blue > visualization too much. Does anyone else have any suggestions? > Thanks, > Eva > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ********************************************************************************* > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient, please delete it > and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned > and although no computer viruses were detected, The Childrens Hospital > at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. > > ********************************************************************************* > > > > ------------------------------ > > Message: 13 > Date: Tue, 12 Apr 2011 23:22:21 +0000 > From: Tony Henwood > Subject: RE: [Histonet] Von kossa stain > To: "'Breeden, Sara'" , "Webb, Dorothy L" > , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E95A@xmdb02.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Interesting, > > Meloan & Puchtler (1985) have drawn our attention to Von Kossa's > original work on this technique. He regarded only the yellow > colouration of calcium deposits during early stages of the reaction as > diagnostic for calcium phosphate and credited the blackening to > organic matter. Further studies showed that bright light only causes > the irreversible blackening of organic matter that masks the yellow > silver phosphate. When the reaction is performed in subdued light, > yellow to yellowish brown silver phosphate is visualised selectively. > Silver carbonate dissolves in sodium thiosulphate and cannot be > demonstrated with von Kossa's technique (Meloan & Puchtler 1985 J > Histotechnol 8(1):11-13.). > > Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), > FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 > Fax: 612 9845 3318 the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden, Sara > Sent: Wednesday, 13 April 2011 3:53 AM > To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Von kossa stain > > Funny you should ask... just last week I had two requests for a Von > Kossa. That's when I found out that, despite the fact that we just > moved into a brand new building with all the bells and whistles, there > was not one single UV light in any of the many hoods that had been > installed. Heaven forbid I could even find an incandescent bulb (we > are a "green building"). So, I prepped my slides, put them in a clear > glass Coplin jar and parked the jar on the hood of my car for an hour. > > Works like a charm. This all depends, naturally, on (1) if you even > HAVE sunshine where you live; (2) how far a walk it is to the > sunshine, and (3) whether you have a car hood on which to park the > Coplin jar. > Needless to say, out of the line of sight of curious onlookers with > sticky fingers and no business wondering what that glass jar is... > Nonetheless, it works just fine! May the Force be with you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ********************************************************************************* > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient, please delete it > and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned > and although no computer viruses were detected, The Childrens Hospital > at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. > > ********************************************************************************* > > > > ------------------------------ > > Message: 14 > Date: Tue, 12 Apr 2011 23:30:30 +0000 > From: Tony Henwood > Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor > To: "'Evans, Andria B'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E981@xmdb02.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Increase the fixation time and make sure the air is out of the sponges > (will stop formalin from getting to the tissue- a quick dunk of the > cassette (with sponge and tissue) in alcohol and back into formalin > will do the trick). > > Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), > FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 > Fax: 612 9845 3318 the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, > Andria B > Sent: Wednesday, 13 April 2011 4:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor > > Our lab is currently looking for a way to shorten our Biopsy > processing program without compromising the patient specimen. We do > have an issue with our GI's being very dry, which causes us to have to > soak between each level taken and also causes a lot of chatter. Also > we have a goal to do a run during the day to improve turn around time. > Here is what our current protocol is.... > > Formalin 1 min 37degrees > Formalin 15 mins 37degrees > 70 14 mins 40 degrees > 95 14 mins 40 degrees > 95 9 mins 40 degrees > 100 9 mins 40 degrees > 100 7 mins 40 degrees > 100 4 mins 40 degrees > Xylene 23 mins no heat > Xylene 15 mins no heat > Paraffin 20 mins 60 degrees > Paraffin 18 mins 60 degrees > Paraffin 10 mins 60 degrees > Paraffin 0 mins 60 degrees > > All the steps are set on a fast mix setting. All of our biopsy > specimens are put into sponges. > > Any feedback would be greatly appreciated. > > Andria B Evans HTL(ASCP)CM > Lancaster General Hospital > 555 North Duke Street > Lancaster, PA 17604 > (717)544-5511 ext: 77329 > aevans3@lgheath.org > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ********************************************************************************* > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient, please delete it > and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned > and although no computer viruses were detected, The Childrens Hospital > at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. > > ********************************************************************************* > > > > ------------------------------ > > Message: 15 > Date: Tue, 12 Apr 2011 17:58:36 -0600 > From: WILLIAM DESALVO > Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6 > Processor > To: , , histonet > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > I would consider replacing the sponges. Tony is correct that you need > to make sure the air is removed from the sponge to facilitate > exchange, but with shortened processing times you will undoubtedly > cause carry over from solution to solution. Yuo may want to have a > small container of alcoholic formalin with sponges sitting on the > gross table. > > Try using a nylon tissue bag to funnel filter your small biopsies. all > your biopsy sample sizes can be placed into the bag and very tiny > samples are retained. Last suggestion is to consider a folded filter > paper method (we name it origami, I can provide directly if you want) > that uses a quick four fold process w/ forceps at the gross bench to > create a pocket for the tiny samples, has a single layer between the > tissue/solution and is easy to open at embedding (without "popping" of > tissue samples). Avoind the unnecessary carryover of the sponge if you > can. > > William DeSalvo, B.S., HTL(ASCP) > > > > > >> From: AnthonyH@chw.edu.au >> To: aevans3@lghealth.org; histonet@lists.utsouthwestern.edu >> Date: Tue, 12 Apr 2011 23:30:30 +0000 >> CC: Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 >> Processor >> >> Increase the fixation time and make sure the air is out of the >> sponges (will stop formalin from getting to the tissue- a quick dunk >> of the cassette (with sponge and tissue) in alcohol and back into >> formalin will do the trick). >> >> Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), >> FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 >> Fax: 612 9845 3318 the children's hospital at westmead >> Cnr Hawkesbury Road and Hainsworth Street, Westmead >> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Evans, Andria B >> Sent: Wednesday, 13 April 2011 4:57 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor >> >> Our lab is currently looking for a way to shorten our Biopsy >> processing program without compromising the patient specimen. We do >> have an issue with our GI's being very dry, which causes us to have >> to soak between each level taken and also causes a lot of chatter. >> Also we have a goal to do a run during the day to improve turn around >> time. Here is what our current protocol is.... >> >> Formalin 1 min 37degrees >> Formalin 15 mins 37degrees >> 70 14 mins 40 degrees >> 95 14 mins 40 degrees >> 95 9 mins 40 degrees >> 100 9 mins 40 degrees >> 100 7 mins 40 degrees >> 100 4 mins 40 degrees >> Xylene 23 mins no heat >> Xylene 15 mins no heat >> Paraffin 20 mins 60 degrees >> Paraffin 18 mins 60 degrees >> Paraffin 10 mins 60 degrees >> Paraffin 0 mins 60 degrees >> >> All the steps are set on a fast mix setting. All of our biopsy >> specimens are put into sponges. >> >> Any feedback would be greatly appreciated. >> >> Andria B Evans HTL(ASCP)CM >> Lancaster General Hospital >> 555 North Duke Street >> Lancaster, PA 17604 >> (717)544-5511 ext: 77329 >> aevans3@lgheath.org >> This email was sent securely from the LGHealth Email Service >> >> Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of intended recipient(s) and may >> contain confidential and privileged information. Any unauthorized >> review, use, disclosure or distribution is prohibited. If you are >> not the intended recipient, please contact the sender by reply e-mail >> and destroy all copies of the original message. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ********************************************************************************* >> This email and any files transmitted with it are confidential and >> intended solely for the use of the individual or entity to whom they >> are addressed. If you are not the intended recipient, please delete >> it and notify the sender. >> >> Views expressed in this message and any attachments are those of the >> individual sender, and are not necessarily the views of The >> Children's Hospital at Westmead >> >> This note also confirms that this email message has been virus >> scanned and although no computer viruses were detected, The Childrens >> Hospital at Westmead accepts no liability for any consequential >> damage resulting from email containing computer viruses. > >> >> ********************************************************************************* >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 16 > Date: Wed, 13 Apr 2011 00:10:04 +0000 > From: Tony Henwood > Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6 > Processor > To: "'WILLIAM DESALVO'" , > "aevans3@lghealth.org" , histonet > > Message-ID: <6D6BD1DE8A5571489398B392A38A71571884E9D3@xmdb02.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > I agree > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] > Sent: Wednesday, 13 April 2011 9:59 AM > To: Tony Henwood; aevans3@lghealth.org; histonet > Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6 > Processor > > I would consider replacing the sponges. Tony is correct that you need > to make sure the air is removed from the sponge to facilitate > exchange, but with shortened processing times you will undoubtedly > cause carry over from solution to solution. Yuo may want to have a > small container of alcoholic formalin with sponges sitting on the > gross table. > > Try using a nylon tissue bag to funnel filter your small biopsies. all > your biopsy sample sizes can be placed into the bag and very tiny > samples are retained. Last suggestion is to consider a folded filter > paper method (we name it origami, I can provide directly if you want) > that uses a quick four fold process w/ forceps at the gross bench to > create a pocket for the tiny samples, has a single layer between the > tissue/solution and is easy to open at embedding (without "popping" of > tissue samples). Avoind the unnecessary carryover of the sponge if you > can. > > William DeSalvo, B.S., HTL(ASCP) > > > > > >> From: AnthonyH@chw.edu.au >> To: aevans3@lghealth.org; >> histonet@lists.utsouthwestern.edu >> Date: Tue, 12 Apr 2011 23:30:30 +0000 >> CC: >> Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor >> >> Increase the fixation time and make sure the air is out of the >> sponges (will stop formalin from getting to the tissue- a quick dunk >> of the cassette (with sponge and tissue) in alcohol and back into >> formalin will do the trick). >> >> Regards >> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) >> Laboratory Manager & Senior Scientist >> Tel: 612 9845 3306 >> Fax: 612 9845 3318 >> the children's hospital at westmead >> Cnr Hawkesbury Road and Hainsworth Street, Westmead >> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA >> >> >> -----Original Message----- >> From: >> histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Evans, Andria B >> Sent: Wednesday, 13 April 2011 4:57 AM >> To: >> histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor >> >> Our lab is currently looking for a way to shorten our Biopsy >> processing program without compromising the patient specimen. We do >> have an issue with our GI's being very dry, which causes us to have >> to soak between each level taken and also causes a lot of chatter. >> Also we have a goal to do a run during the day to improve turn around >> time. Here is what our current protocol is.... >> >> Formalin 1 min 37degrees >> Formalin 15 mins 37degrees >> 70 14 mins 40 degrees >> 95 14 mins 40 degrees >> 95 9 mins 40 degrees >> 100 9 mins 40 degrees >> 100 7 mins 40 degrees >> 100 4 mins 40 degrees >> Xylene 23 mins no heat >> Xylene 15 mins no heat >> Paraffin 20 mins 60 degrees >> Paraffin 18 mins 60 degrees >> Paraffin 10 mins 60 degrees >> Paraffin 0 mins 60 degrees >> >> All the steps are set on a fast mix setting. All of our biopsy >> specimens are put into sponges. >> >> Any feedback would be greatly appreciated. >> >> Andria B Evans HTL(ASCP)CM >> Lancaster General Hospital >> 555 North Duke Street >> Lancaster, PA 17604 >> (717)544-5511 ext: 77329 >> >> aevans3@lgheath.org> >> This email was sent securely from the LGHealth Email Service >> >> Confidentiality Notice: >> This e-mail message, including any attachments, is for the sole use >> of intended recipient(s) and may contain confidential and privileged >> information. >> Any unauthorized review, use, disclosure or distribution is >> prohibited. >> If you are not the intended recipient, please contact the sender by >> reply e-mail and destroy all copies of the original message. >> >> _______________________________________________ >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ********************************************************************************* >> This email and any files transmitted with it are confidential and >> intended solely for the use of the individual or entity to whom they >> are addressed. If you are not the intended recipient, please delete >> it and notify the sender. >> >> Views expressed in this message and any attachments are those of the >> individual sender, and are not necessarily the views of The >> Children's Hospital at Westmead >> >> This note also confirms that this email message has been virus >> scanned and although no computer viruses were detected, The Childrens >> Hospital at Westmead accepts no liability for any consequential >> damage resulting from email containing computer viruses. > >> >> ********************************************************************************* >> >> _______________________________________________ >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 17 > Date: Tue, 12 Apr 2011 19:25:30 -0500 > From: Mahesh Polavarapu > Subject: [Histonet] Update regarding question on Plastic Embedding > To: histonet > Message-ID: > Content-Type: text/plain; charset=ISO-8859-1 > > Looking for a protocol to visualize vascularization and collagen > deposition > at the bone-tendon interface of a rabbit rotator cuff embedded in a > plastic > system. Sections will be rather thick (~50um) b/c they are being made > through a titanium anchor. Using MMA with a cold-curing resin, > Technovit > 9100. Thanks in advance! > > - Mahesh > > > ------------------------------ > > Message: 18 > Date: Tue, 12 Apr 2011 20:34:51 -0400 > From: "Richard Cartun" > Subject: [Histonet] SP3 HER2 mAb > To: "Histonet" > Message-ID: <4DA4B76A.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Anyone using the HER2 rabbit monoclonal antibody (clone SP3) ever see > reactivity with skeletal muscle? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > > > ------------------------------ > > Message: 19 > Date: Tue, 12 Apr 2011 23:14:30 -0400 > From: Eugenia Thomas > Subject: [Histonet] FW: 1 H&E slide vs. 2 > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > > > > From: eugenia902d1@hotmail.com > To: histonet@lists.utsouthwestern.edu > Subject: 1 H&E slide vs. 2 > Date: Mon, 11 Apr 2011 13:06:59 -0400 > > > > > Good afternoon everyone, > Does anyone know of an article or statistics discussing the impact > (medical diagnosing) of cutting 2 H&E slides per block verses 1 for > all routine work? > Genia > > ------------------------------ > > Message: 20 > Date: Wed, 13 Apr 2011 08:39:22 -0500 > From: > Subject: [Histonet] Ventana ultra > To: > Message-ID: > > <7DA79EBDBD92BF408EF392413737878D3936B06565@NADCWPMSGCMS01.hca.corpad.net> > > Content-Type: text/plain; charset="us-ascii" > > Does anyone use the Benchmark Ultra - care to share your comments? > > We are considering switching from the Benchmark XT to the Ultra but > would like to hear from users about this instrument. > Is there an increase in reagent cost? > > Can you really add more slides without adding time to the run? > > > ANTOINETTE CRILL, MBA,CT(ASCP) > TEAM LEADER ANATOMIC PATHOLOGY > DANVILLE REGIONAL MEDICAL CENTER > (O) 434.799.4470 > (F) 434.773.6806 > E-mail: barbara.crill@LPNT.net > > > > ------------------------------ > > Message: 21 > Date: Wed, 13 Apr 2011 10:00:09 -0400 > From: "Monfils, Paul" > Subject: RE: [Histonet] FW: 1 H&E slide vs. 2 > To: > Message-ID: > > <4EBFF65383B74D49995298C4976D1D5E08020E55@LSRIEXCH1.lsmaster.lifespan.org> > > Content-Type: text/plain; charset="us-ascii" > > When I was in clinical histology (I'm in research now) the > pathologists > didn't even want us putting two sections on a slide, because even > though > they knew a second serial section wasn't going to show anything the > first section didn't show, they felt ethically/legally bound to > examine > both sections if they were there, so they were investing twice the > time > and effort for the same return. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Eugenia > Thomas > Sent: Tuesday, April 12, 2011 11:15 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FW: 1 H&E slide vs. 2 > > > > > > From: eugenia902d1@hotmail.com > To: histonet@lists.utsouthwestern.edu > Subject: 1 H&E slide vs. 2 > Date: Mon, 11 Apr 2011 13:06:59 -0400 > > > > > Good afternoon everyone, > Does anyone know of an article or statistics discussing the impact > (medical diagnosing) of cutting 2 H&E slides per block verses 1 for > all > routine work? > Genia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 22 > Date: Wed, 13 Apr 2011 09:09:22 -0500 > From: "Sebree Linda A" > Subject: RE: [Histonet] Ventana ultra > To: , > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > Please "Reply All" as I'd like to hear feedback also. > > Thanks. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Barbara.Crill@LPNT.net > Sent: Wednesday, April 13, 2011 8:39 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ventana ultra > > Does anyone use the Benchmark Ultra - care to share your comments? > > We are considering switching from the Benchmark XT to the Ultra but > would like to hear from users about this instrument. > Is there an increase in reagent cost? > > Can you really add more slides without adding time to the run? > > > ANTOINETTE CRILL, MBA,CT(ASCP) > TEAM LEADER ANATOMIC PATHOLOGY > DANVILLE REGIONAL MEDICAL CENTER > (O) 434.799.4470 > (F) 434.773.6806 > E-mail: barbara.crill@LPNT.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 89, Issue 13 > **************************************** > > > This information is confidential and intended solely for the use of > the individual or entity to whom it is addressed. If you have > received this email in error please notify the sender or our Customer > Support Center at (262) 928-2777. We have scanned this e-mail and its > attachments for malicious content. However, the recipient should > check this email and any attachments for the presence of viruses. > ProHealth Care accepts no liability for any damage caused by any virus > transmitted by this email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From awatanabe <@t> tgen.org Sat Apr 16 19:29:17 2011 From: awatanabe <@t> tgen.org (awatanabe@tgen.org) Date: Sat Apr 16 19:29:21 2011 Subject: [Histonet] Sectioning undecalcified bone Message-ID: <4EA62CF152D9974BA99A28A456C9B2D101C335@EX-MBX1.ad.tgen.org> I have a sample that is 3 needle bone biopsies that I need to get an H&E and subsequently several 12um thick sections for further assays. The bone appears to not be decaled at all. I don't normally have bone to cut so I'm looking for suggestions. Aprill From tissuearray <@t> hotmail.com Sat Apr 16 23:07:11 2011 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Sat Apr 16 23:07:18 2011 Subject: [Histonet] Preparing a Tissue Microarray Block for Cutting Message-ID: Hi All, I found this video on the Arraymold.com website and thought it would be good information for anyone preparing a TMAs block for cutting. Heres the link: http://www.arraymold.com/video4.html Brad From histotech <@t> imagesbyhopper.com Sun Apr 17 09:37:27 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun Apr 17 09:37:34 2011 Subject: [Histonet] Control Tissue Message-ID: <001f01cbfd0d$032cf030$0986d090$@imagesbyhopper.com> Hi Histonetters! I have been requested to post a question to the group at large: Is there any way to process a "control tissue" in a tissue processor, with the hopes of showing that the processor is working up to par? Perhaps someone has a procedure for this sort of thing? I know we use known controls for stains, but how would one accomplish this sort of thing with a tissue processor? TIA for any insight! Michelle From histotech <@t> imagesbyhopper.com Sun Apr 17 09:38:06 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun Apr 17 09:37:58 2011 Subject: [Histonet] Control slides for H&E Message-ID: <002401cbfd0d$1156bb50$340431f0$@imagesbyhopper.com> How often do you use a H&E control slide per day? Do you run it once after the stainer has been changed, once with each rack put on the stainer, after "x" number of racks etc? Does anyone have a specific procedure they would like to share for this QC step? Thanks! Michelle From rjbuesa <@t> yahoo.com Sun Apr 17 09:40:59 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 17 09:41:02 2011 Subject: [Histonet] Sectioning undecalcified bone In-Reply-To: <4EA62CF152D9974BA99A28A456C9B2D101C335@EX-MBX1.ad.tgen.org> Message-ID: <683385.3131.qm@web65706.mail.ac4.yahoo.com> You will have to decalcify the bone first. Being so small pieces, I advise you to use EDTA and proceed with the regular tissue processing after the bone is decalcified. Ren? J. --- On Sat, 4/16/11, awatanabe@tgen.org wrote: From: awatanabe@tgen.org Subject: [Histonet] Sectioning undecalcified bone To: histonet@lists.utsouthwestern.edu Date: Saturday, April 16, 2011, 8:29 PM I have a sample that is 3 needle bone biopsies that I need to get an H&E and subsequently several 12um thick sections for further assays.? The bone appears to not be decaled at all.? I don't normally have bone to cut so I'm looking for suggestions. Aprill _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Apr 17 09:47:28 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 17 09:47:33 2011 Subject: [Histonet] Control Tissue In-Reply-To: <001f01cbfd0d$032cf030$0986d090$@imagesbyhopper.com> Message-ID: <110644.64914.qm@web65716.mail.ac4.yahoo.com> A tissue processor is "working up to par" as long as the processing protocol you use is adequate. Meaning that the tissue processor does what you tell it to do. If with your protocol you are able to obtain a well infiltrated tissue, that is the objective. Each tissue contains "internal controls", use them as a reference. Ren? J. --- On Sun, 4/17/11, histotech@imagesbyhopper.com wrote: From: histotech@imagesbyhopper.com Subject: [Histonet] Control Tissue To: histonet@lists.utsouthwestern.edu Date: Sunday, April 17, 2011, 10:37 AM Hi Histonetters! I have been requested to post a question to the group at large:? Is there any way to process a "control tissue" in a tissue processor, with the hopes of showing that the processor is working up to par?? Perhaps someone has a procedure for this sort of thing?? I know we use known controls for stains, but how would one accomplish this sort of thing with a tissue processor? TIA for any insight! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Apr 17 09:49:43 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 17 09:49:47 2011 Subject: [Histonet] Control slides for H&E In-Reply-To: <002401cbfd0d$1156bb50$340431f0$@imagesbyhopper.com> Message-ID: <426381.87522.qm@web65710.mail.ac4.yahoo.com> Every day, as the reagents have been changed, use a (+) control and file it with the date. Try to use a tissue that contains most of the types of cells you will encounter during the day. An appendix section will do. Ren? J. --- On Sun, 4/17/11, histotech@imagesbyhopper.com wrote: From: histotech@imagesbyhopper.com Subject: [Histonet] Control slides for H&E To: histonet@lists.utsouthwestern.edu Date: Sunday, April 17, 2011, 10:38 AM How often do you use a H&E control slide per day?? Do you run it once after the stainer has been changed, once with each rack put on the stainer, after "x" number of racks etc?? Does anyone have a specific procedure they would like to share for this QC step? Thanks! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun Apr 17 19:04:14 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Apr 17 19:04:30 2011 Subject: [Histonet] un-nucleated squamous epithelial cells In-Reply-To: <248540.17036.qm@web1104.biz.mail.sk1.yahoo.com> References: <724571.5966.qm@web65713.mail.ac4.yahoo.com> <248540.17036.qm@web1104.biz.mail.sk1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71571884FBC4@xmdb02.nch.kids> So does that mean I will have to wear makeup? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Friday, 15 April 2011 5:53 AM To: Rene J Buesa; Histonet Subject: Re: [Histonet] un-nucleated squamous epithelial cells If lotion gets in the waterbath, the tissue will not stick to the slides. Shed skin cells can fall from any exposed skin, not just the hands.?Like from the face of those that do not wear make-up or lotion. ? Paula ________________________________ From: Rene J Buesa To: "Histonet (histonet@lists.utsouthwestern.edu)" ; RogerCharles Sent: Thu, April 14, 2011 2:22:41 PM Subject: Re: [Histonet] un-nucleated squamous epithelial cells Any extra-dried skin can shed cells. The best option is having your HTs using some skin lotion (like Keri lotion) to prevent shedding of cells. Ren? J. --- On Thu, 4/14/11, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] un-nucleated squamous epithelial cells To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Thursday, April 14, 2011, 2:56 PM Hello, Has anyone studied where un-nucleated squamous epithelial cells originate from????I'm leaning towards "thumbing blocks" with an ungloved hand but don't know for sure.? Other possibilities I've come up with are dandruff from the head or uncovered arms.? I hate to see our histologist work in a complete tyvek outfit so I'm searching for clues. Thanks to all. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From histotech411 <@t> gmail.com Sun Apr 17 20:51:57 2011 From: histotech411 <@t> gmail.com (Jenny Vega) Date: Sun Apr 17 20:52:00 2011 Subject: [Histonet] Question about delayed start in Leica Tissue Processor Message-ID: Hello. I have little experience using the tissue processor from my lab which is an old version of the Leica TP1020. I was taugh that since on weekends the processor is not going to be used then you have to set up the days of delay for example 2-18:20 (2 represents saturday and sunday). On Monday (tomorrow) I have the day off, so the processor won't be used. When I come back on Tuesday I have to change the the time as 0-18:20 so that the processor starts working that day and on wednesday. On thursday, friday, saturday and Sunday will also have the days off. I am confused because my supevisor told me to set the time as 3-18:20 next Wednesday, and since I will not be working in the lab for 4 days straight that does not make sense, it is suppose to be 4-18:20 instead. I think she made a mistake telling me that since maybe she confused the program of this week when the processor was programmed as 3-18:20 because used for 3 days straight (saturday to monday). My supervisor won't be in the lab next week so I cannot ask her. This question seems silly but I want to be sure. I know that if tissue is left for a long time in paraffin the tissue can get over-harden and I don't want to program de delayed start as 3-18:20 thinking it is the correct way when is not. Thanks From Saro.Bascaramurty <@t> nrc-cnrc.gc.ca Mon Apr 18 08:32:50 2011 From: Saro.Bascaramurty <@t> nrc-cnrc.gc.ca (Bascaramurty, Saro) Date: Mon Apr 18 08:32:59 2011 Subject: [Histonet] Question about delayed start in Leica Tissue Processor In-Reply-To: References: Message-ID: She is right. Day 0 is today (the same day you start the run) -day 1 Day 1 is tomorrow start the run the next day,- day 2 Day 2 is the day after tomorrow - day 3 Day 3 is the following day -day 4 and so on -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: April 17, 2011 8:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about delayed start in Leica Tissue Processor Hello. I have little experience using the tissue processor from my lab which is an old version of the Leica TP1020. I was taugh that since on weekends the processor is not going to be used then you have to set up the days of delay for example 2-18:20 (2 represents saturday and sunday). On Monday (tomorrow) I have the day off, so the processor won't be used. When I come back on Tuesday I have to change the the time as 0-18:20 so that the processor starts working that day and on wednesday. On thursday, friday, saturday and Sunday will also have the days off. I am confused because my supevisor told me to set the time as 3-18:20 next Wednesday, and since I will not be working in the lab for 4 days straight that does not make sense, it is suppose to be 4-18:20 instead. I think she made a mistake telling me that since maybe she confused the program of this week when the processor was programmed as 3-18:20 because used for 3 days straight (saturday to monday). My supervisor won't be in the lab next week so I cannot ask her. This question seems silly but I want to be sure. I know that if tissue is left for a long time in paraffin the tissue can get over-harden and I don't want to program de delayed start as 3-18:20 thinking it is the correct way when is not. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Apr 18 08:38:17 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Apr 18 08:38:26 2011 Subject: [Histonet] un-nucleated squamous epithelial cells In-Reply-To: <6D6BD1DE8A5571489398B392A38A71571884FBC4@xmdb02.nch.kids> References: <724571.5966.qm@web65713.mail.ac4.yahoo.com> <248540.17036.qm@web1104.biz.mail.sk1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71571884FBC4@xmdb02.nch.kids> Message-ID: <659319.47669.qm@web1101.biz.mail.sk1.yahoo.com> LOL, only if?tastefully done. I'm just saying, a couple dead epi cells are something that should be overlooked, as they cannot be avoided and are the main component of household dust. The stray clothing?or paper fiber is another nuisance. ? Paula ________________________________ From: Tony Henwood To: Paula Pierce ; Rene J Buesa ; Histonet Sent: Sun, April 17, 2011 7:04:14 PM Subject: RE: [Histonet] un-nucleated squamous epithelial cells So does that mean I will have to wear makeup? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Friday, 15 April 2011 5:53 AM To: Rene J Buesa; Histonet Subject: Re: [Histonet] un-nucleated squamous epithelial cells If lotion gets in the waterbath, the tissue will not stick to the slides. Shed skin cells can fall from any exposed skin, not just the hands.?Like from the face of those that do not wear make-up or lotion. ? Paula ________________________________ From: Rene J Buesa To: "Histonet (histonet@lists.utsouthwestern.edu)" ; RogerCharles Sent: Thu, April 14, 2011 2:22:41 PM Subject: Re: [Histonet] un-nucleated squamous epithelial cells Any extra-dried skin can shed cells. The best option is having your HTs using some skin lotion (like Keri lotion) to prevent shedding of cells. Ren? J. --- On Thu, 4/14/11, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] un-nucleated squamous epithelial cells To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Thursday, April 14, 2011, 2:56 PM Hello, Has anyone studied where un-nucleated squamous epithelial cells originate from????I'm leaning towards "thumbing blocks" with an ungloved hand but don't know for sure.? Other possibilities I've come up with are dandruff from the head or uncovered arms.? I hate to see our histologist work in a complete tyvek outfit so I'm searching for clues. Thanks to all. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From nto <@t> stowers.org Mon Apr 18 08:55:18 2011 From: nto <@t> stowers.org (Thomas, Nancy) Date: Mon Apr 18 08:55:34 2011 Subject: [Histonet] Question about delayed start in Leica Tissue Processor In-Reply-To: References: Message-ID: So that makes 4 the correct number because she is starting it on a Wednesday (day 0) Thurs. =1,Fri.=2, Sat.=3, Sun=4 and then it would start that night at 18:20. Sun. would be included (according to the manual) because the count refers to how many times the clock passes by midnight. (Wed., Thurs, Fri, and Sat. nights) Nancy Thomas -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bascaramurty, Saro Sent: Monday, April 18, 2011 8:33 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question about delayed start in Leica Tissue Processor She is right. Day 0 is today (the same day you start the run) -day 1 Day 1 is tomorrow start the run the next day,- day 2 Day 2 is the day after tomorrow - day 3 Day 3 is the following day -day 4 and so on -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: April 17, 2011 8:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about delayed start in Leica Tissue Processor Hello. I have little experience using the tissue processor from my lab which is an old version of the Leica TP1020. I was taugh that since on weekends the processor is not going to be used then you have to set up the days of delay for example 2-18:20 (2 represents saturday and sunday). On Monday (tomorrow) I have the day off, so the processor won't be used. When I come back on Tuesday I have to change the the time as 0-18:20 so that the processor starts working that day and on wednesday. On thursday, friday, saturday and Sunday will also have the days off. I am confused because my supevisor told me to set the time as 3-18:20 next Wednesday, and since I will not be working in the lab for 4 days straight that does not make sense, it is suppose to be 4-18:20 instead. I think she made a mistake telling me that since maybe she confused the program of this week when the processor was programmed as 3-18:20 because used for 3 days straight (saturday to monday). My supervisor won't be in the lab next week so I cannot ask her. This question seems silly but I want to be sure. I know that if tissue is left for a long time in paraffin the tissue can get over-harden and I don't want to program de delayed start as 3-18:20 thinking it is the correct way when is not. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Mon Apr 18 09:16:38 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Mon Apr 18 09:16:51 2011 Subject: [Histonet] H& E SLIDES Message-ID: Histonetters Another question about H &E control slides how long do you keep them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From rjbuesa <@t> yahoo.com Mon Apr 18 10:05:27 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 18 10:05:34 2011 Subject: [Histonet] H& E SLIDES In-Reply-To: Message-ID: <279591.80882.qm@web65710.mail.ac4.yahoo.com> The same time you have to keep your "case slides" because in the event of "legal dispute" where the staining may become "an issue" you have to produce the H&E control used along with the batch including the "disputed case". Ren? J. --- On Mon, 4/18/11, Sara Baldwin/mhhcc.org wrote: From: Sara Baldwin/mhhcc.org Subject: [Histonet] H& E SLIDES To: histonet@lists.utsouthwestern.edu Date: Monday, April 18, 2011, 10:16 AM Histonetters Another question about H &E control slides how long do you keep them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216,? Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Apr 18 10:23:09 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Apr 18 10:23:15 2011 Subject: [Histonet] H& E SLIDES In-Reply-To: <279591.80882.qm@web65710.mail.ac4.yahoo.com> Message-ID: I would say the same time as you keep your other QC records. CAP requires QC records to be kept for 2 years. Your state or individual institution may have longer retention times. "Quality control records, including instrument printouts: at least 2 years." Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 04/18/2011 08:08 AM To histonet@lists.utsouthwestern.edu, "Sara Baldwin/mhhcc.org" cc Subject Re: [Histonet] H& E SLIDES The same time you have to keep your "case slides" because in the event of "legal dispute" where the staining may become "an issue" you have to produce the H&E control used along with the batch including the "disputed case". Ren? J. --- On Mon, 4/18/11, Sara Baldwin/mhhcc.org wrote: From: Sara Baldwin/mhhcc.org Subject: [Histonet] H& E SLIDES To: histonet@lists.utsouthwestern.edu Date: Monday, April 18, 2011, 10:16 AM Histonetters Another question about H &E control slides how long do you keep them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Mon Apr 18 10:28:04 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Apr 18 10:28:02 2011 Subject: [Histonet] QC records In-Reply-To: References: <279591.80882.qm@web65710.mail.ac4.yahoo.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B370407F044@giamail2.Gia.com> I was wondering how long to keep all my QC log sheets...so CAP is 2 years? Does anyone know about CLIA's rules? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, April 18, 2011 10:23 AM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] H& E SLIDES I would say the same time as you keep your other QC records. CAP requires QC records to be kept for 2 years. Your state or individual institution may have longer retention times. "Quality control records, including instrument printouts: at least 2 years." Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 04/18/2011 08:08 AM To histonet@lists.utsouthwestern.edu, "Sara Baldwin/mhhcc.org" cc Subject Re: [Histonet] H& E SLIDES The same time you have to keep your "case slides" because in the event of "legal dispute" where the staining may become "an issue" you have to produce the H&E control used along with the batch including the "disputed case". Ren? J. --- On Mon, 4/18/11, Sara Baldwin/mhhcc.org wrote: From: Sara Baldwin/mhhcc.org Subject: [Histonet] H& E SLIDES To: histonet@lists.utsouthwestern.edu Date: Monday, April 18, 2011, 10:16 AM Histonetters Another question about H &E control slides how long do you keep them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech411 <@t> gmail.com Mon Apr 18 11:24:10 2011 From: histotech411 <@t> gmail.com (Jenny Vega) Date: Mon Apr 18 11:24:14 2011 Subject: [Histonet] Question about delayed start in Leica Tissue Processor In-Reply-To: References: Message-ID: Yes, that is what makes sense to me. Tomorrow morning after removing the specimens from the tissue basket I will check how my supervisor set the clock last Friday. If she chose 3-18:20, then by logic I will have to chose 4-18:20, since I will be away for the holidays for 4 days straight and I need the tissues to be processed by the next monday (april 25). I was told that the tissue processor starts at 6pm and it is done the next morning. I did not understood military time before and now I know that 18:20 means 6:20pm. Thanks On Mon, Apr 18, 2011 at 8:39 AM, Thomas, Nancy wrote: > We have the same processor. If you are returning to work next Monday > morning, then you would choose the 4 day delay. It would begin processing > at 18:20 Sunday night and be ready on Monday morning. > Nancy Thomas > Stowers Institute > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega > Sent: Sunday, April 17, 2011 8:52 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question about delayed start in Leica Tissue Processor > > Hello. I have little experience using the tissue processor from my lab > which is an old version of the Leica TP1020. I was taugh that since on > weekends the processor is not going to be used then you have to set up the > days of delay for example 2-18:20 (2 represents saturday and sunday). On > Monday > (tomorrow) I have the day off, so the processor won't be used. When I come > back on Tuesday I have to change the the time as 0-18:20 so that the > processor starts working that day and on wednesday. On thursday, friday, > saturday and Sunday will also have the days off. I am confused because my > supevisor told me to set the time as 3-18:20 next Wednesday, and since I > will not be working in the lab for 4 days straight that does not make sense, > it is suppose to be 4-18:20 instead. I think she made a mistake telling me > that since maybe she confused the program of this week when the processor > was programmed as 3-18:20 because used for 3 days straight (saturday to > monday). My supervisor won't be in the lab next week so I cannot ask her. > > This question seems silly but I want to be sure. I know that if tissue is > left for a long time in paraffin the tissue can get over-harden and I don't > want to program de delayed start as 3-18:20 thinking it is the correct way > when is not. > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald <@t> mtsac.edu Mon Apr 18 11:37:22 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Apr 18 11:37:29 2011 Subject: [Histonet] QC records In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370407F044@giamail2.Gia.com> Message-ID: Same to my knowledge. "Amber McKenzie" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/18/2011 08:30 AM To "Jennifer MacDonald" , "Rene J Buesa" cc histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Subject [Histonet] QC records I was wondering how long to keep all my QC log sheets...so CAP is 2 years? Does anyone know about CLIA's rules? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, April 18, 2011 10:23 AM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] H& E SLIDES I would say the same time as you keep your other QC records. CAP requires QC records to be kept for 2 years. Your state or individual institution may have longer retention times. "Quality control records, including instrument printouts: at least 2 years." Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 04/18/2011 08:08 AM To histonet@lists.utsouthwestern.edu, "Sara Baldwin/mhhcc.org" cc Subject Re: [Histonet] H& E SLIDES The same time you have to keep your "case slides" because in the event of "legal dispute" where the staining may become "an issue" you have to produce the H&E control used along with the batch including the "disputed case". Ren? J. --- On Mon, 4/18/11, Sara Baldwin/mhhcc.org wrote: From: Sara Baldwin/mhhcc.org Subject: [Histonet] H& E SLIDES To: histonet@lists.utsouthwestern.edu Date: Monday, April 18, 2011, 10:16 AM Histonetters Another question about H &E control slides how long do you keep them? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Mon Apr 18 13:10:07 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Apr 18 13:14:27 2011 Subject: [Histonet] Cassette/Slide labelers.... again.... Message-ID: Hello all, Is anyone using the ID/Positive system from Data General? Pros? Cons? Any info would be helpful. Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From anonwums1 <@t> gmail.com Mon Apr 18 14:33:53 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Apr 18 14:33:57 2011 Subject: [Histonet] Xylene re-use SOP Message-ID: Hi all, Our small research lab recently has been investigated by a very aggressive environmental health and safety inspector, and she asked us to write up the standard of practice for any chemicals that we re-use, including all the chemicals we re-use for deparaffinization and dehydration and rehydration of slides. We currently keep a few bottles of xylene and graded ethanol that we dispense into staining racks used for deparaffinizing and rehydration of sections. We pour the bottles back into their containers once we're done with them and reuse them until it has a lot of paraffin detritus in it. We do this all by hand. EH&S wants us to do the following: If a container is labeled "in process" or "recycled" be sure to have an SOP written up describing what is being done and what is meant by "recycled" (in this case a solution is being used again). Be sure to say at what point the solution is waste and then how it is managed after that (properly labeled and disposed). Don't label containers ?waste? or ?used? unless it is labeled with a properly filled out yellow hazardous waste sticker provided by EH&S. I have no idea what they're looking for, but they've gotten quite strict in enforcing all sorts of vague regulations. Any suggestions are welcome. Thanks, Adam From anonwums1 <@t> gmail.com Mon Apr 18 15:54:28 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Apr 18 15:54:32 2011 Subject: [Histonet] Re: Xylene re-use SOP In-Reply-To: References: Message-ID: Just to answer a few more questions people asked. We do not use a solvent recycler. Once our xylene is "bad," we label it with a sticker and EH&S picks it up and disposes of it somehow. The graduate student who started this would just eyeball the xylene and if it was gunky, replace it with fresh xylene from a big vat stored in a lab down the hall. I have no idea how many slides it would take to get to that point, or how many slides we should be processing through it before it stops working. I would guess that we go through somewhere between 50 - 100 slides a month, and that's on a busy month. We replace it 3 times a year or so. How many slides should I be processing through this? Adam On Mon, Apr 18, 2011 at 2:33 PM, Adam . wrote: > Hi all, > > Our small research lab recently has been investigated by a very aggressive > environmental health and safety inspector, and she asked us to write up the > standard of practice for any chemicals that we re-use, including all the > chemicals we re-use for deparaffinization and dehydration and rehydration of > slides. We currently keep a few bottles of xylene and graded ethanol that we > dispense into staining racks used for deparaffinizing and rehydration of > sections. We pour the bottles back into their containers once we're done > with them and reuse them until it has a lot of paraffin detritus in it. We > do this all by hand. > > EH&S wants us to do the following: > > If a container is labeled "in process" or "recycled" be sure to have an SOP > written up describing what is being done and what is meant by "recycled" (in > this case a solution is being used again). Be sure to say at what point the > solution is waste and then how it is managed after that (properly labeled > and disposed). Don't label containers ?waste? or ?used? unless it is labeled > with a properly filled out yellow hazardous waste sticker provided by EH&S. > > I have no idea what they're looking for, but they've gotten quite strict in > enforcing all sorts of vague regulations. Any suggestions are welcome. > > Thanks, > Adam > From amber.mckenzie <@t> gastrodocs.net Mon Apr 18 16:06:56 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Apr 18 16:06:54 2011 Subject: [Histonet] thermometer In-Reply-To: References: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B37040C93DF@giamail2.Gia.com> My last CLIA inspection, I was told to check the temperature in the wax chamber of my embedding station to compare against the temperature it says it is...does anyone know of a good thermometer to purchase? From histonet.nospam <@t> vneubert.com Tue Apr 19 01:17:23 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Apr 19 01:17:34 2011 Subject: [Histonet] thermometer In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B37040C93DF@giamail2.Gia.com> References: <03C921A1EAF7F541B16543F6EC6A4B37040C93DF@giamail2.Gia.com> Message-ID: <4DAD28F3.4080803@vneubert.com> A pyrometer should do the job, I guess. > My last CLIA inspection, I was told to check the temperature in the wax > chamber of my embedding station to compare against the temperature it > says it is...does anyone know of a good thermometer to purchase? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Tue Apr 19 01:43:55 2011 From: louise.renton <@t> gmail.com (Louise Renton) Date: Tue Apr 19 01:44:00 2011 Subject: [Histonet] Question about delayed start in Leica Tissue Processor In-Reply-To: References: Message-ID: The easiest way to remember is to count the "midnights" between starting and ending - therefore today will be "0", tomorrow "1"etc On Mon, Apr 18, 2011 at 6:24 PM, Jenny Vega wrote: > Yes, that is what makes sense to me. Tomorrow morning after removing the > specimens from the tissue basket I will check how my supervisor set the > clock last Friday. If she chose 3-18:20, then by logic I will have to chose > 4-18:20, since I will be away for the holidays for 4 days straight and I > need the tissues to be processed by the next monday (april 25). I was told > that the tissue processor starts at 6pm and it is done the next morning. I > did not understood military time before and now I know that 18:20 means > 6:20pm. > > Thanks > > On Mon, Apr 18, 2011 at 8:39 AM, Thomas, Nancy wrote: > > > We have the same processor. If you are returning to work next Monday > > morning, then you would choose the 4 day delay. It would begin > processing > > at 18:20 Sunday night and be ready on Monday morning. > > Nancy Thomas > > Stowers Institute > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega > > Sent: Sunday, April 17, 2011 8:52 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Question about delayed start in Leica Tissue > Processor > > > > Hello. I have little experience using the tissue processor from my lab > > which is an old version of the Leica TP1020. I was taugh that since on > > weekends the processor is not going to be used then you have to set up > the > > days of delay for example 2-18:20 (2 represents saturday and sunday). On > > Monday > > (tomorrow) I have the day off, so the processor won't be used. When I > come > > back on Tuesday I have to change the the time as 0-18:20 so that the > > processor starts working that day and on wednesday. On thursday, friday, > > saturday and Sunday will also have the days off. I am confused because my > > supevisor told me to set the time as 3-18:20 next Wednesday, and since I > > will not be working in the lab for 4 days straight that does not make > sense, > > it is suppose to be 4-18:20 instead. I think she made a mistake telling > me > > that since maybe she confused the program of this week when the processor > > was programmed as 3-18:20 because used for 3 days straight (saturday to > > monday). My supervisor won't be in the lab next week so I cannot ask her. > > > > This question seems silly but I want to be sure. I know that if tissue is > > left for a long time in paraffin the tissue can get over-harden and I > don't > > want to program de delayed start as 3-18:20 thinking it is the correct > way > > when is not. > > > > Thanks > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From A.Mortimer <@t> latrobe.edu.au Tue Apr 19 04:00:27 2011 From: A.Mortimer <@t> latrobe.edu.au (Abbey Mortimer) Date: Tue Apr 19 04:00:35 2011 Subject: [Histonet] Tissue adhesion to slides Message-ID: Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.mortimer@latrobe.edu.au From esulkosky <@t> gmail.com Tue Apr 19 07:20:46 2011 From: esulkosky <@t> gmail.com (Eric Sulkosky) Date: Tue Apr 19 07:20:50 2011 Subject: [Histonet] Cytotech job opening in Brooklyn NY Message-ID: There is a full time position open for a Cytotechnologist in Brooklyn, NY. A candidate with Histology background is preferred. Please forward letter of interest and resume to Ellen Barbarino ebarbarino@siradonc.com Thanks From hendrik.mommaerts <@t> med.kuleuven.be Tue Apr 19 08:50:33 2011 From: hendrik.mommaerts <@t> med.kuleuven.be (Hendrik Mommaerts) Date: Tue Apr 19 08:50:42 2011 Subject: [Histonet] Neuronal staining mouse limb sections Message-ID: <005d01cbfe98$c10e3910$432aab30$@mommaerts@med.kuleuven.be> Hello, I'm investigating a potential neuronal defect of our transgenic mouse embryos. Until now the staining I tried (luxol Fast Blue) didn't work out. I was wondering now if there is an alternative for this. Is there a staining (or an Ab), that I can use to detect any kind of neurons in the limb of a mouse embryo? I'm looking for a general detection of neurons. Preferentially I would like it to work on frozen sections, but for now anything would work for me! Thank you! From AGleiberman <@t> cbiolabs.com Tue Apr 19 09:04:17 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue Apr 19 09:04:34 2011 Subject: [Histonet] Neuronal staining mouse limb sections In-Reply-To: <005d01cbfe98$c10e3910$432aab30$@mommaerts@med.kuleuven.be> References: <005d01cbfe98$c10e3910$432aab30$@mommaerts@med.kuleuven.be> Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C162AFF@cbiolabs05.CBiolabs.local> Hi Hendrik, Try rabbit neuro-specific beta-III tubulin antibody (Abcam ab18207). Works fine on frozen and paraffin sections. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hendrik Mommaerts Sent: Tuesday, April 19, 2011 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neuronal staining mouse limb sections Hello, I'm investigating a potential neuronal defect of our transgenic mouse embryos. Until now the staining I tried (luxol Fast Blue) didn't work out. I was wondering now if there is an alternative for this. Is there a staining (or an Ab), that I can use to detect any kind of neurons in the limb of a mouse embryo? I'm looking for a general detection of neurons. Preferentially I would like it to work on frozen sections, but for now anything would work for me! Thank you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From Jane.Moose <@t> NewberryHospital.net Tue Apr 19 09:18:42 2011 From: Jane.Moose <@t> NewberryHospital.net (Jane C. Moose) Date: Tue Apr 19 09:18:47 2011 Subject: [Histonet] IHC on frozen sections Message-ID: <8BB5FC36DDA373488E60177757BBD698ABD47B@ncmhexchbe01.NewberryHospital.net> Following is question on behalf of Jana Moose Ritter, DVM janamoose@yahoo.com We are going to perform IHC on frozen sections, and the tissues (pig aorta) will be harvested on a Friday. Histo lab is wondering: 1) can tissue fix in paraformaldehyde over the weekend until next steps (sucrose and frozen embedding to be completed on Monday) 2) can fixative be switched to sucrose at the end of Friday (tissue would remain in sucrose until frozen embedding on Monday) 3) must complete the solution switch and entire frozen embedding process on Saturday Thanks for your help. Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 From Carmen.M.Garcia <@t> uv.es Tue Apr 19 09:40:18 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Tue Apr 19 09:40:25 2011 Subject: [Histonet] Question Message-ID: <5686474943carmaga6@uv.es> Good afternoon: I am Carmen Garcia a PhD student from the Faculty of medicine from the University of Valencia.We work with cryocut of mouse ovary, now I want to see the filopodia of the endothelial cells, do you have any suggestion about what I should do? Thank you so much, Carmen From BenatM <@t> gosh.nhs.uk Tue Apr 19 09:56:11 2011 From: BenatM <@t> gosh.nhs.uk (Malika Benatti) Date: Tue Apr 19 09:56:25 2011 Subject: [Histonet] Miller's Elastin Stain In-Reply-To: <201104181701.BID29764@rlmh03.swi.contact.secure-ops.net> References: <201104181701.BID29764@rlmh03.swi.contact.secure-ops.net> Message-ID: <4DADB09C.4626.0038.0@gosh.nhs.uk> ** Proprietary ** ** Reply Requested When Convenient ** Hi there, Does anyone use commercial Miller's Elastic Stain other than VWR Ref 35115S one ? If so I would be grateful if you could let me know which one you use Thanks in advance Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 benatm@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* From sfonner <@t> labpath.com Tue Apr 19 09:59:55 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Tue Apr 19 10:02:44 2011 Subject: [Histonet] RE: [IHCRG] Ventana EBER DNP In-Reply-To: <3FBEE959ECE9104B93A3D493D920F6F90886284E@exchange26.nh.novant.net> References: <3FBEE959ECE9104B93A3D493D920F6F90886284E@exchange26.nh.novant.net> Message-ID: <000801cbfea2$71ff87c0$55fe9740$@com> SAME HERE!!! I would love some help with this. We are going to start doing the EBER with Ventana and have no idea where to start. Thanks in advance for ANY suggestions or help you could give. Sheila, HT (ASCP) KDL Pathology Knoxville, TN From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Tallardy, Betty S Sent: Tuesday, April 19, 2011 10:55 AM To: ihcrg@googlegroups.com Subject: [IHCRG] Ventana EBER DNP Hi Group, Is anyone stuck in the EBER transition with Ventanas' probe changeover? The new product is supplied in a small vial and of course there are no suggestions about how to use the product. If anyone has some ideas on how to proceed with this, I would really appreciate some suggestions. I hate to waste such a pricey product trying to get something workable. Thanks in advance. Betty Tallardy, HT(ASCP)QIHC Dept. of Pathology Presbyterian Hospital 200 Hawthorne Lane Charlotte, NC 28204 704-384-5490 bstallardy@novanthealth.org _____ This message and any included attachments are from NOVANT HEALTH INC. and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. If you believe that any information contained in this message is disparaging or harassing or if you find it objectionable please contact Novant Health, Inc. at 1-800-350-0094 or forward the e-mail to reports@novanthealth.org. Thank you. -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From marktarango <@t> gmail.com Tue Apr 19 10:08:47 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Apr 19 10:08:51 2011 Subject: [Histonet] RE: [IHCRG] Ventana EBER DNP In-Reply-To: <000801cbfea2$71ff87c0$55fe9740$@com> References: <3FBEE959ECE9104B93A3D493D920F6F90886284E@exchange26.nh.novant.net> <000801cbfea2$71ff87c0$55fe9740$@com> Message-ID: If anyone has the catalog numbers for the probes, that would help. I couldn't get this info from Ventana when I asked. They said we have to ask another lab, even for catalog numbers! Mark On Tue, Apr 19, 2011 at 7:59 AM, Sheila Fonner wrote: > SAME HERE!!! I would love some help with this. We are going to start > doing > the EBER with Ventana and have no idea where to start. > > > > Thanks in advance for ANY suggestions or help you could give. > > > > Sheila, HT (ASCP) > > KDL Pathology > > Knoxville, TN > > > > > > From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of > Tallardy, Betty S > Sent: Tuesday, April 19, 2011 10:55 AM > To: ihcrg@googlegroups.com > Subject: [IHCRG] Ventana EBER DNP > > > > Hi Group, > > Is anyone stuck in the EBER transition with Ventanas' > > probe changeover? The new product is supplied in a small vial and of > course > there are no suggestions about how to use the product. > > If anyone has some ideas on how to proceed with this, I would really > appreciate some suggestions. > > I hate to waste such a pricey product trying to get something workable. > > Thanks in advance. > > Betty Tallardy, HT(ASCP)QIHC > > Dept. of Pathology > > Presbyterian Hospital > > 200 Hawthorne Lane > > Charlotte, NC 28204 > > 704-384-5490 > > bstallardy@novanthealth.org > > _____ > > This message and any included attachments are from NOVANT HEALTH INC. and > are intended only for the addressee(s). The information contained herein > may > include trade secrets or privileged or otherwise confidential information. > Unauthorized review, forwarding, printing, copying, distributing, or using > such information is strictly prohibited and may be unlawful. If you > received > this message in error, or have reason to believe you are not authorized to > receive it, please promptly delete this message and notify the sender by > e-mail. If you believe that any information contained in this message is > disparaging or harassing or if you find it objectionable please contact > Novant Health, Inc. at 1-800-350-0094 or forward the e-mail to > reports@novanthealth.org. Thank you. > > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee within > the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: histo@nsh.orgor > call 443.535.4060. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akbitting <@t> geisinger.edu Tue Apr 19 10:42:42 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Apr 19 10:42:50 2011 Subject: [Histonet] RE: [IHCRG] Ventana EBER DNP In-Reply-To: References: <3FBEE959ECE9104B93A3D493D920F6F90886284E@exchange26.nh.novant.net> <000801cbfea2$71ff87c0$55fe9740$@com> Message-ID: <4DAD7532.2B7F.00C9.1@geisinger.edu> Your sales rep should supply these to you. I'm having problems with filling my ISH Probe Kit. I logged in my EBER Probe. I registered the Probe kit and went to fill it and it only lets me pick from my Fillable Reagents list, not the Fillable probes list. Tech support can't figure out why this is happening and said they haven't encountered this problem with any other clients. Anyone else having this issue? >>> Mark Tarango 4/19/2011 11:08 AM >>> If anyone has the catalog numbers for the probes, that would help. I couldn't get this info from Ventana when I asked. They said we have to ask another lab, even for catalog numbers! Mark On Tue, Apr 19, 2011 at 7:59 AM, Sheila Fonner wrote: > SAME HERE!!! I would love some help with this. We are going to start > doing > the EBER with Ventana and have no idea where to start. > > > > Thanks in advance for ANY suggestions or help you could give. > > > > Sheila, HT (ASCP) > > KDL Pathology > > Knoxville, TN > > > > > > From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of > Tallardy, Betty S > Sent: Tuesday, April 19, 2011 10:55 AM > To: ihcrg@googlegroups.com > Subject: [IHCRG] Ventana EBER DNP > > > > Hi Group, > > Is anyone stuck in the EBER transition with Ventanas' > > probe changeover? The new product is supplied in a small vial and of > course > there are no suggestions about how to use the product. > > If anyone has some ideas on how to proceed with this, I would really > appreciate some suggestions. > > I hate to waste such a pricey product trying to get something workable. > > Thanks in advance. > > Betty Tallardy, HT(ASCP)QIHC > > Dept. of Pathology > > Presbyterian Hospital > > 200 Hawthorne Lane > > Charlotte, NC 28204 > > 704-384-5490 > > bstallardy@novanthealth.org > > _____ > > This message and any included attachments are from NOVANT HEALTH INC. and > are intended only for the addressee(s). The information contained herein > may > include trade secrets or privileged or otherwise confidential information. > Unauthorized review, forwarding, printing, copying, distributing, or using > such information is strictly prohibited and may be unlawful. If you > received > this message in error, or have reason to believe you are not authorized to > receive it, please promptly delete this message and notify the sender by > e-mail. If you believe that any information contained in this message is > disparaging or harassing or if you find it objectionable please contact > Novant Health, Inc. at 1-800-350-0094 or forward the e-mail to > reports@novanthealth.org. Thank you. > > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee within > the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: histo@nsh.orgor > call 443.535.4060. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From TNMayer <@t> mdanderson.org Tue Apr 19 10:47:28 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Apr 19 10:47:45 2011 Subject: [Histonet] [Histonet Biopsy Program for Sakura VIP 5/6 Processor In-Reply-To: <44519b9a-7013-4bcf-bff6-05da2a11063a@DCPWPRTR02.mdanderson.edu> References: <44519b9a-7013-4bcf-bff6-05da2a11063a@DCPWPRTR02.mdanderson.edu> Message-ID: Try to eliminate one of the 100% Alcohols. If it cannot eliminate it, then just pump it in and out. Microwave processing actually uses Ethanol, Isopropyl, and paraffin only. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org > Message: 8 > Date: Tue, 12 Apr 2011 14:57:18 -0400 > From: "Evans, Andria B" > Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60@MAIL-AG-CLUSTER.lha.org> > Content-Type: text/plain; charset="iso-8859-1" > > Our lab is currently looking for a way to shorten our Biopsy > processing program without compromising the patient specimen. We do > have an issue with our GI's being very dry, which causes us to have to > soak between each level taken and also causes a lot of chatter. Also > we have a goal to do a run during the day to improve turn around time. > Here is what our current protocol is.... > > Formalin 1 min 37degrees > Formalin 15 mins 37degrees > 70 14 mins 40 degrees > 95 14 mins 40 degrees > 95 9 mins 40 degrees > 100 9 mins 40 degrees > 100 7 mins 40 degrees > 100 4 mins 40 degrees > Xylene 23 mins no heat > Xylene 15 mins no heat > Paraffin 20 mins 60 degrees > Paraffin 18 mins 60 degrees > Paraffin 10 mins 60 degrees > Paraffin 0 mins 60 degrees > > All the steps are set on a fast mix setting. All of our biopsy > specimens are put into sponges. > > Any feedback would be greatly appreciated. > > Andria B Evans HTL(ASCP)CM > Lancaster General Hospital > 555 North Duke Street > Lancaster, PA 17604 > (717)544-5511 ext: 77329 > aevans3@lgheath.org From rjbuesa <@t> yahoo.com Tue Apr 19 11:36:42 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 19 11:36:45 2011 Subject: [Histonet] thermometer In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B37040C93DF@giamail2.Gia.com> Message-ID: <968066.7018.qm@web65713.mail.ac4.yahoo.com> You can bay a certified digital thermometer for less than $35 and will be more than adequate for what you need. Ren? J. --- On Mon, 4/18/11, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] thermometer To: histonet@lists.utsouthwestern.edu Date: Monday, April 18, 2011, 5:06 PM My last CLIA inspection, I was told to check the temperature in the wax chamber of my embedding station to compare against the temperature it says it is...does anyone know of a good thermometer to purchase? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 19 11:38:46 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 19 11:38:51 2011 Subject: [Histonet] Tissue adhesion to slides In-Reply-To: Message-ID: <645914.14905.qm@web65710.mail.ac4.yahoo.com> Such thick sections are usually handled better if stained floating (not adhered to sledes). Ren? J. --- On Tue, 4/19/11, Abbey Mortimer wrote: From: Abbey Mortimer Subject: [Histonet] Tissue adhesion to slides To: "Histonet@lists.utsouthwestern.edu" Date: Tuesday, April 19, 2011, 5:00 AM Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.mortimer@latrobe.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 19 11:41:29 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 19 11:41:33 2011 Subject: [Histonet] [Histonet Biopsy Program for Sakura VIP 5/6 Processor In-Reply-To: Message-ID: <925230.5666.qm@web65709.mail.ac4.yahoo.com> Incidentally, using ethanol + isopropanol?makes no sense and has no chemical fundament. Results are better using isopropanol ONLY. Ren? J. --- On Tue, 4/19/11, Mayer,Toysha N wrote: From: Mayer,Toysha N Subject: [Histonet] [Histonet Biopsy Program for Sakura VIP 5/6 Processor To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, April 19, 2011, 11:47 AM Try to eliminate one of the 100% Alcohols.? If it cannot eliminate it, then just pump it in and out. Microwave processing actually uses Ethanol, Isopropyl, and paraffin only. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org > Message: 8 > Date: Tue, 12 Apr 2011 14:57:18 -0400 > From: "Evans, Andria B" > Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor > To: "histonet@lists.utsouthwestern.edu" > ??? > Message-ID: > ????? > <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60@MAIL-AG-CLUSTER.lha.org> > Content-Type: text/plain;??? charset="iso-8859-1" > > Our lab is currently looking for a way to shorten our Biopsy > processing program without compromising the patient specimen.? We do > have an issue with our GI's being very dry, which causes us to have to > soak between each level taken and also causes a lot of chatter.? Also > we have a goal to do a run during the day to improve turn around time. > Here is what our current protocol is.... > > Formalin? ? 1 min? ? ???37degrees > Formalin? ? 15 mins? ? 37degrees > 70? ? ? ? ? ???14 mins? ? 40 degrees > 95? ? ? ? ? ???14 mins? ? 40 degrees > 95? ? ? ? ? ???9 mins? ? ? 40 degrees > 100? ? ? ? ???9 mins? ? ? 40 degrees > 100? ? ? ? ???7 mins? ? ? 40 degrees > 100? ? ? ? ???4 mins? ? ? 40 degrees > Xylene? ? ???23 mins? ? no heat > Xylene? ? ???15 mins? ? no heat > Paraffin? ? ? 20 mins???60 degrees > Paraffin? ? ? 18 mins???60 degrees > Paraffin? ? ? 10 mins???60 degrees > Paraffin? ? ? 0 mins? ???60 degrees > > All the steps are set on a fast mix setting.? All of our biopsy > specimens are put into sponges. > > Any feedback would be greatly appreciated. > > Andria B Evans HTL(ASCP)CM > Lancaster General Hospital > 555 North Duke Street > Lancaster, PA? 17604 > (717)544-5511 ext: 77329 > aevans3@lgheath.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Candy.A.Bales <@t> uth.tmc.edu Tue Apr 19 11:51:30 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Tue Apr 19 11:51:34 2011 Subject: [Histonet] in-situ hybridization Message-ID: <62C915811DD5A142851D95CA6BC5D1E41B374593AC@UTHCMS1.uthouston.edu> This questions is for the labs that do in-situ hybridization for diagnostic purposes. Do you take slides for ISH from other labs and if so, please contact me via my email. My next question is; one of my doc's is interested in us doing ISH herein our lab but I have been putting her off with the fact that we do not need this very often, it is very expensive to start up, etc. But she is persistent. Any suggestions for starting up this procedure, including vendor preferences for supplies? I know next to nothing about ISH other than what I have read on-line. Thank you in advance Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From Mandy.Bell <@t> chomp.org Tue Apr 19 12:13:27 2011 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Tue Apr 19 12:15:08 2011 Subject: [Histonet] slide drying ovens Message-ID: I'm looking for recommendations for a new slide drying oven. Something relatively small, with convection. Any input would be appreciated. Thanks ! Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail From estellamireles <@t> gmail.com Tue Apr 19 12:22:33 2011 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Tue Apr 19 12:22:38 2011 Subject: [Histonet] Disposing of gross only items Message-ID: Hello Everyone, Need some info on the disposal of gross only items. These items include hardware, wires, gallstones and foreign objects. We are running out of room for the storage of gross only specimens. From akemiat3377 <@t> yahoo.com Tue Apr 19 12:24:47 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Apr 19 12:25:12 2011 Subject: [Histonet] slide drying ovens In-Reply-To: References: Message-ID: <3BE3B8A3-8C91-44B6-867A-D9281D20CD18@yahoo.com> There are many so called convection ovens out there that are gravity convection. Biocare has a great convection oven that has an internal fan, and can be set with several different programs. You can set it for IHC at the end of the day and it automatically stops and is ready for you in the AM when you come in. It has a small footprint. It is not cheap, but well worth it. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Apr 19, 2011, at 10:13 AM, Bell, Mandy wrote: > I'm looking for recommendations for a new slide drying oven. > Something relatively small, with convection. Any input would be > appreciated. Thanks ! > > Mandy M Bell , HTL (ASCP) > Histology Department > Community Hospital of the Monterey Peninsula > 831.625.4791 > > > P Please consider the environment before printing this e-mail > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laura_conner <@t> hotmail.com Tue Apr 19 12:32:52 2011 From: laura_conner <@t> hotmail.com (Laura Conner) Date: Tue Apr 19 12:32:56 2011 Subject: [Histonet] Rabbit eye holder? Message-ID: Hi all, I'm looking for something to hold my rabbit eyes in place (and other eyes if they're available like rat) while cutting them in half before further processing. My boss said he used them a long time ago but doesn't know what they're called. I can't figure out what they'd be called either but I'd like to buy some if they exist. Any input? Ideas? Thanks! Laura From akemiat3377 <@t> yahoo.com Tue Apr 19 12:36:35 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Apr 19 12:37:00 2011 Subject: [Histonet] 3, 000 Breast Cancer Specimens from Quebec Breast Cancer being retested Message-ID: <81EB75AE-35C5-4587-B776-A718E01C8FE1@yahoo.com> PhenoPath Laboratories Retests Nearly 3,000 Breast Cancer Specimens from Quebec Breast Cancer specimens which may have been misdiagnosed. In the re-analysis, results of which were just announced, a total of 87 women were found to have received a false negative result from the original pathology laboratory tests in Quebec. Of this number, 39 were required to alter their treatment and among them five women died. Minister of Health Yves Bolduc said it was impossible to determine whether the women died as a result of being improperly treated. Check out this link. http://www.ihc.co/a824362-seattle-based- phenopath-laboratories-retests-nearly-.cfm Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From Janice.Mahoney <@t> alegent.org Tue Apr 19 12:57:36 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Apr 19 12:57:43 2011 Subject: [Histonet] unsubscribe Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C75D9@EXCHMBC2.ad.ah.local> ________________________________ Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From talulahgosh <@t> gmail.com Tue Apr 19 13:12:53 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Apr 19 13:12:56 2011 Subject: [Histonet] in-situ hybridization In-Reply-To: <62C915811DD5A142851D95CA6BC5D1E41B374593AC@UTHCMS1.uthouston.edu> References: <62C915811DD5A142851D95CA6BC5D1E41B374593AC@UTHCMS1.uthouston.edu> Message-ID: Roche Diagnostics has great DIG labels, antibodies and color reaction chemicals. It is a pain to just start up, you need to buy a lot of stuff for it. Could you use someone else's set up and pay for part of the chemicals? Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 19, 2011 at 12:51 PM, Bales, Candy A wrote: > This questions is for the labs that do in-situ hybridization for diagnostic > purposes. Do you take slides for ISH from other labs and if so, please > contact me via my email. > > My next question is; one of my doc's is interested in us doing ISH herein > our lab but I have been putting her off with the fact that we do not need > this very often, it is very expensive to start up, etc. > But she is persistent. Any suggestions for starting up this procedure, > including vendor preferences for supplies? I know next to nothing about ISH > other than what I have read on-line. > > Thank you in advance > Candy > > > > Candy Bales, HT > Chief Histologist > The University of Texas Dental Branch at Houston > Diagnostic Sciences-Oral Pathology > 6516 M.D. Anderson Blvd. # 3.093 > Houston, TX 77030 > 713.500.4411 office > 713.500.4416 fax > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Tue Apr 19 13:35:23 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Apr 19 13:35:47 2011 Subject: [Histonet] Re: Standardization??? 3, 000 Breast Cancer Specimens from Quebec Breast Cancer being retested In-Reply-To: <81EB75AE-35C5-4587-B776-A718E01C8FE1@yahoo.com> References: <81EB75AE-35C5-4587-B776-A718E01C8FE1@yahoo.com> Message-ID: <990F8BFB-E0B8-48E5-8228-6B566CE8D9EA@yahoo.com> From what I have experienced in my observations of various labs; numerous labs do not follow proper standardization practices which are set by FDA guidelines for Her2-neu. Prognostic markers are particularly susceptible to heat. Baking slides in excess temperatures (over 62 degrees C) for prolonged lengths of time can also drop the antigenicity of the test tissues a full grade. There has been documentation of this through publications. Temperatures MUST be monotored and recorded. The Antigen Retrieval Process is very important and QC measures MUST be followed. Internal temperature and pressure MUST be met and recorded. QC monitor strips will record and show any irregularities in this step. If the pressure and temp has not been met, the test should be repeated. A lot of IHC techs do not monitor this process. Unfortunately, internal laboratory standardization can vary from shift to shift. Just think about non- standardization practices throughout the country because of under- education and strict adherence to established guidelines. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Apr 19, 2011, at 10:36 AM, Akemi Allison wrote: > PhenoPath Laboratories Retests Nearly 3,000 Breast Cancer Specimens > from Quebec Breast Cancer specimens which may have been > misdiagnosed. In the re-analysis, results of which were just > announced, a total of 87 women were found to have received a false > negative result from the original pathology laboratory tests in > Quebec. Of this number, 39 were required to alter their treatment > and among them five women died. Minister of Health Yves Bolduc said > it was impossible to determine whether the women died as a result > of being improperly treated. Check out this link. http://www.ihc.co/ > a824362-seattle-based-phenopath-laboratories-retests-nearly-.cfm > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Tue Apr 19 13:43:44 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Tue Apr 19 13:44:50 2011 Subject: [Histonet] Re: Histonet Biopsy Program for Sakura VIP 5/6 Processor Message-ID: Wow, somehow I missed this email. Sorry for the delay. Andria, take the heat off your alcohols. Keep it on the formalin and the paraffin, but do the rest of the processing at ambient temperature. Make sure you put only small soft tissues on this schedule though, tougher collagenous skin specimens might not process well enough with the quick dehydration times you have. I am curious how you arrived at the timing for the steps. 14 minutes, 9 minutes, 7 minutes, 23 minutes, etc? Good luck and best wishes, Teri Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From Mary.L.Ring <@t> HealthPartners.Com Tue Apr 19 14:04:32 2011 From: Mary.L.Ring <@t> HealthPartners.Com (Ring, Mary L) Date: Tue Apr 19 14:04:36 2011 Subject: [Histonet] IHC validation Message-ID: <2594D39B69223047B6E2F4ADF1E62DB9010FFB487841@HPEMX3.HealthPartners.int> Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From kiran_g <@t> sbcglobal.net Tue Apr 19 14:30:36 2011 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Tue Apr 19 14:30:39 2011 Subject: [Histonet] Tissue Microarray Message-ID: <583100.88978.qm@web180113.mail.gq1.yahoo.com> Are you using tissue microarrays as controls for all your routine IHC stains (not antibody validation or research) ? If so, how are you making the arrays? ? thank you, ? -Kiranjit From rjbuesa <@t> yahoo.com Tue Apr 19 14:32:30 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 19 14:32:33 2011 Subject: [Histonet] Disposing of gross only items In-Reply-To: Message-ID: <304082.70528.qm@web65716.mail.ac4.yahoo.com> First talk to your legal department, just in case! Ren? J. --- On Tue, 4/19/11, Stella Mireles wrote: From: Stella Mireles Subject: [Histonet] Disposing of gross only items To: Histonet@lists.utsouthwestern.edu Date: Tuesday, April 19, 2011, 1:22 PM Hello Everyone, Need some info on the disposal of gross only items. These items include hardware, wires, gallstones and foreign objects. We are running out of room for the storage of gross only specimens. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 19 14:36:02 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 19 14:36:05 2011 Subject: [Histonet] IHC validation In-Reply-To: <2594D39B69223047B6E2F4ADF1E62DB9010FFB487841@HPEMX3.HealthPartners.int> Message-ID: <478173.881.qm@web65715.mail.ac4.yahoo.com> Not really because the Hematoxylin should "affect" only the nuclei UNLESS it is a nuclear antibody, in which case the?antigenic reaction?could be obscured. For nuclear Abs I used to dilute my hematoxylin as much as possible or did not use it at all. Ren? J. --- On Tue, 4/19/11, Ring, Mary L wrote: From: Ring, Mary L Subject: [Histonet] IHC validation To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, April 19, 2011, 3:04 PM Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Apr 19 14:39:57 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Apr 19 14:40:08 2011 Subject: [Histonet] IHC validation In-Reply-To: <2594D39B69223047B6E2F4ADF1E62DB9010FFB487841@HPEMX3.HealthPartners.int> References: <2594D39B69223047B6E2F4ADF1E62DB9010FFB487841@HPEMX3.HealthPartners.int> Message-ID: <4DADACCD.7400.0077.1@harthosp.org> In my opinion, only if it affects immunoreactivity. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Ring, Mary L" 4/19/2011 3:04 PM >>> Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CKent <@t> mbhs.org Tue Apr 19 16:02:02 2011 From: CKent <@t> mbhs.org (CKent@mbhs.org) Date: Tue Apr 19 16:02:08 2011 Subject: [Histonet] AUTO: Carolyn J Kent is out of the office. (returning 04/21/2011) Message-ID: I am out of the office until 04/21/2011. I will respond to your message when I return. Note: This is an automated response to your message "Histonet Digest, Vol 89, Issue 21" sent on 4/19/2011 12:02:59 PM. This is the only notification you will receive while this person is away. From kstoll <@t> mcw.edu Tue Apr 19 16:23:31 2011 From: kstoll <@t> mcw.edu (Stoll, Kathryn) Date: Tue Apr 19 16:22:20 2011 Subject: [Histonet] Vendor for antibodies - research Message-ID: <110E7925E2B91945A9B79EDFD0DC2B34E2371E90F6@MCWMBX2.mcwcorp.net> I am switching from hospital based IHC to research in a new lab. I am hoping to get some input for which vendors have shown good results for antibodies that I have not previously used. I am looking for a few specific ENT-1 RRM-1 ERCC TYMS SPARC TOPO-1 Thanks From billodonnell <@t> catholichealth.net Tue Apr 19 15:05:52 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Apr 19 16:25:56 2011 Subject: [Histonet] slide drying ovens In-Reply-To: <3BE3B8A3-8C91-44B6-867A-D9281D20CD18@yahoo.com> References: <3BE3B8A3-8C91-44B6-867A-D9281D20CD18@yahoo.com> Message-ID: I agree w Akemi, the BioCare oven is top-notch and programmable in ways that other ovens cannot even touch. A little complicated to set-up, but the folks at BioCare will walk you through it. Once programmed, it's a breeze to run. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Tuesday, April 19, 2011 12:25 PM To: Bell, Mandy; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] slide drying ovens There are many so called convection ovens out there that are gravity convection. Biocare has a great convection oven that has an internal fan, and can be set with several different programs. You can set it for IHC at the end of the day and it automatically stops and is ready for you in the AM when you come in. It has a small footprint. It is not cheap, but well worth it. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Apr 19, 2011, at 10:13 AM, Bell, Mandy wrote: > I'm looking for recommendations for a new slide drying oven. > Something relatively small, with convection. Any input would be > appreciated. Thanks ! > > Mandy M Bell , HTL (ASCP) > Histology Department > Community Hospital of the Monterey Peninsula > 831.625.4791 > > > P Please consider the environment before printing this e-mail > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dw18 <@t> uchicago.edu Tue Apr 19 16:41:12 2011 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Tue Apr 19 16:41:18 2011 Subject: [Histonet] whole retinal staining Message-ID: <20110419164112.ANU62452@mstore03.uchicago.edu> Dear Abbey & Histonet I agree with Ren? Buesa: I would try to stain whole retinae by a 'floating section' method i.e. hand processing in a dipping tray or mesh baskets (brush transfer can be done too but is laborious). Then mount them at the end. Floating allows reagents to penetrate from both sides of the retina - extra valuable because your incubation times are going to be very long with material this thick. (I assume you are not just interested in superficial layers.) A microwave processor would help with penetration, but I have no direct experience. -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery ORIGINAL POST: Histonet Digest, Vol 89, Issue 21 Message: 7 Date: Tue, 19 Apr 2011 19:00:27 +1000 From: Abbey Mortimer Subject: [Histonet] Tissue adhesion to slides Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.mortimer@latrobe.edu.au From AnthonyH <@t> chw.edu.au Tue Apr 19 18:03:23 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 19 18:03:36 2011 Subject: [Histonet] RE: IHC on frozen sections In-Reply-To: <8BB5FC36DDA373488E60177757BBD698ABD47B@ncmhexchbe01.NewberryHospital.net> References: <8BB5FC36DDA373488E60177757BBD698ABD47B@ncmhexchbe01.NewberryHospital.net> Message-ID: <6D6BD1DE8A5571489398B392A38A7157188514BE@xmdb02.nch.kids> Jana, Since you are fixing in formaldehyde, why not paraffin embed them? Frozen sectioning of formalin fixed tissue and doing IPX on them is difficult as well as problems with storing the tissue. I would cut frozen of unfixed tissue and do IPX on them rather than doing IPX on formalin fixed frozen sections. Any reason why you have chosen the frozen section of formalin fixed tissue? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: Wednesday, 20 April 2011 12:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on frozen sections Following is question on behalf of Jana Moose Ritter, DVM janamoose@yahoo.com We are going to perform IHC on frozen sections, and the tissues (pig aorta) will be harvested on a Friday. Histo lab is wondering: 1) can tissue fix in paraformaldehyde over the weekend until next steps (sucrose and frozen embedding to be completed on Monday) 2) can fixative be switched to sucrose at the end of Friday (tissue would remain in sucrose until frozen embedding on Monday) 3) must complete the solution switch and entire frozen embedding process on Saturday Thanks for your help. Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From carl.hobbs <@t> kcl.ac.uk Wed Apr 20 03:11:14 2011 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Apr 20 03:11:48 2011 Subject: [Histonet] re: IHC on frozen sections Message-ID: <11D9615B89C10747B1C985966A63D7CA367AA53922@KCL-MAIL04.kclad.ds.kcl.ac.uk> 1) NO 2) Yes...at 4C 3) Sure, if somebody is around to do this....or you want to make somebody go in and do it ;-) carl carl Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases School of Biomedical Sciences King's College London Guys Campus London SE1 1UL Tel.020 7848 6810 fax 020 7848 6816 carl.hobbs@kcl.ac.uk From GauchV <@t> mail.amc.edu Wed Apr 20 07:30:34 2011 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Wed Apr 20 07:30:40 2011 Subject: [Histonet] Looking for position in Baltimore,MD Message-ID: Hi everyone, I have a wonderful tech who is relocating to the Baltimore, MD area and is searching for a Histotech position. If anyone knows of anything, please contact me at this e-mail address (she is not on Histonet currently) and I will get the information to her promptly. Whoever hires this person will be very very happy- she is very conscientious, reliable, hard working and VERY pleasant to work with..we are going to miss her like crazy... Thanks in advance for your help, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From stewart_georgia <@t> sbcglobal.net Wed Apr 20 07:34:20 2011 From: stewart_georgia <@t> sbcglobal.net (Georgia Stewart) Date: Wed Apr 20 07:34:23 2011 Subject: [Histonet] Histonet - unsubscribe Message-ID: <672722.47368.qm@web81402.mail.mud.yahoo.com> Unsubscribe from Histonet. ? Thank you Georgia Stewart ? From histonet.nospam <@t> vneubert.com Wed Apr 20 07:36:54 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Apr 20 07:37:04 2011 Subject: [Histonet] Histonet - unsubscribe In-Reply-To: <672722.47368.qm@web81402.mail.mud.yahoo.com> References: <672722.47368.qm@web81402.mail.mud.yahoo.com> Message-ID: <4DAED366.3000802@vneubert.com> Okay, bye bye :) But do not forget to visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet and fill out the form on the bottom of the page! > Unsubscribe from Histonet. > > Thank you > Georgia Stewart > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nyilmaz <@t> mersin.edu.tr Wed Apr 20 09:12:25 2011 From: nyilmaz <@t> mersin.edu.tr (=?iso-8859-9?Q?Nejat_Y=FDlmaz?=) Date: Wed Apr 20 09:12:23 2011 Subject: [Histonet] decalcification of articular cartilage Message-ID: Dear Members, We're planning to study sheep knee articular cartilage with histochemistry and immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde at the moment. We need an effective, quick and cartilage tissue protective decalcification method. We'll perform only light microscopic examination in this study and we have to complete the study within one week. In these circumstances what are your advices? Thanks in advance. Dr. Necat Yilmaz University of Mersin School of Medicine __________ ESET NOD32 Antivirus Ak?ll? G?venlik taraf?ndan sa?lanan bilgiler, vir?s imza veritaban? s?r?m?: 6057 (20110420) __________ ?leti ESET NOD32 Antivirus Ak?ll? G?venlik taraf?ndan denetlendi. http://www.nod32.com.tr From hchidgey <@t> earthlink.net Wed Apr 20 09:42:59 2011 From: hchidgey <@t> earthlink.net (Henry Chidgey) Date: Wed Apr 20 09:43:27 2011 Subject: [Histonet] Request help on finding a part for Leitz 1512 Message-ID: <00ac01cbff69$3fac5a60$bf050f20$@net> Howdy, I am looking for a replacement screws for the two used to clamp the blade in place on the Leitz 1512. It also might be helpful if someone knows the particulars (thread, diameter. Length) of those screws. Thanks, Henry See what folks are saying about us Wildlife Analytical Laboratories www.DeerAge.com PO Box 295 1303 CR 118 B Burnet, Texas 78611 (512) 756-1989 (512) 663-9476 Cell Serving Wildlife Stewards, Ranchers & Hunters Worldwide Customer Service and Quality of Analysis are our Priority and Passion From hchidgey <@t> earthlink.net Wed Apr 20 11:06:55 2011 From: hchidgey <@t> earthlink.net (Henry Chidgey) Date: Wed Apr 20 11:07:28 2011 Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 Message-ID: <00fe01cbff74$fbbd0870$f3371950$@net> Thanks for all the prompt, complete help. Looks like we will now be able to get our 1512 back in perfect condition. Henry From lbustamante <@t> cvm.tamu.edu Wed Apr 20 11:30:29 2011 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Wed Apr 20 11:30:52 2011 Subject: [Histonet] Manual Microtome Message-ID: <4DAEC3D5020000B9000FDA94@CVM.TAMU.EDU> Dear All. I am looking to buy 4 used (but in very good conditions) non automated microtome that will be used for teaching. If you have any, please send me the maker, model and reasonable price. Thank you very much. Lin Bustamante. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 From gmartin <@t> marshallmedical.org Wed Apr 20 12:12:34 2011 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Apr 20 12:12:41 2011 Subject: [Histonet] Cassette labeler Message-ID: <6ED9D4252F278841A0593D3D788AF24C0D480110@mailsvr.MARSHMED.local> We have just received a Lamb Micro Writer (model # E22.01MWI) single shoe. The problem is that we did not receive an operator manual. Does anyone know where I can get a manual? From polavarapu.mahesh <@t> gmail.com Wed Apr 20 12:24:55 2011 From: polavarapu.mahesh <@t> gmail.com (Mahesh Polavarapu) Date: Wed Apr 20 12:24:59 2011 Subject: [Histonet] 'Deplasticizing' before staining? Message-ID: Hi, I have 50uM thick sections of soft tissue embedded in MMA (cold cured Technovit 9100). Do these need to be 'deplasticized' before they will take up stain? Thanks in advance! - Mahesh From hchidgey <@t> earthlink.net Wed Apr 20 12:31:10 2011 From: hchidgey <@t> earthlink.net (Henry Chidgey) Date: Wed Apr 20 12:31:40 2011 Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 Message-ID: <016f01cbff80$bef19030$3cd4b090$@net> I was asked by a member to share the solution so here it is : Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. He will probably have some or can make you some. Great guy and very knowledgeable. Thanks Joyce for giving me this informationJ Henry From relia1 <@t> earthlink.net Wed Apr 20 12:36:45 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Apr 20 12:36:48 2011 Subject: [Histonet] RELIA Histology Job Alert. Excellent opportunity on Long Island Day shift NY Lic required. Message-ID: Hi Histonetters!! I have a great new opportunity that I want to tell you about. I am working with a Laboratory located on Long Island that is in need of a NY licensed histology tech. This is a dayshift full time permanent position. My client offers a very competitive salary, excellent benefits, a great place to work and they are ready to interview and hire right away. If you or anyone you know might be interested in this position please contact me. I can be reached toll free at 866-607-3542 or relia1@earthlink.net. Thanks and have a great day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From JWeems <@t> sjha.org Wed Apr 20 13:54:58 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Apr 20 13:55:03 2011 Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 In-Reply-To: <016f01cbff80$bef19030$3cd4b090$@net> References: <016f01cbff80$bef19030$3cd4b090$@net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F4BB962@CHEXCMS10.one.ads.che.org> He worked in the Leica factory and is truly an expert. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Henry Chidgey Sent: Wednesday, April 20, 2011 13:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 I was asked by a member to share the solution so here it is : Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. He will probably have some or can make you some. Great guy and very knowledgeable. Thanks Joyce for giving me this informationJ Henry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From algranth <@t> email.arizona.edu Wed Apr 20 14:51:48 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Apr 20 14:52:18 2011 Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 In-Reply-To: <016f01cbff80$bef19030$3cd4b090$@net> References: <016f01cbff80$bef19030$3cd4b090$@net> Message-ID: I second getting in touch with Klaus - he knows the 1512 inside and out. Andi Grantham On Apr 20, 2011, at 10:31 AM, Henry Chidgey wrote: > I was asked by a member to share the solution so here it is : > > Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. > He will probably have some or can make you some. > > Great guy and very knowledgeable. Thanks Joyce for giving me this > informationJ > > Henry > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jcox90 <@t> yahoo.com Wed Apr 20 15:08:11 2011 From: jcox90 <@t> yahoo.com (jcox90@yahoo.com) Date: Wed Apr 20 15:08:15 2011 Subject: [Histonet] Part time Histo Tech position in Phoenix AZ Message-ID: <58759.56388.qm@web161609.mail.bf1.yahoo.com> Part time Histo Tech position open with Southwest Skin Specialists, Ltd., located in Phoenix AZ.? Responsibilities for this position include: grossing and fixation, processing, embedding, sectioning and staining.??? Education Requirements: ?Associate Degree in Applied Science/Histology and/or Histologic Certificate and HT or HTL ASCP Certification.? If interested please send resume to miverson@swskin.net. ? Jill Cox, HT ASCP From spanish_fly1981 <@t> yahoo.com Wed Apr 20 18:36:01 2011 From: spanish_fly1981 <@t> yahoo.com (Tate Maria) Date: Wed Apr 20 18:36:06 2011 Subject: [Histonet] Unsubscribe Message-ID: <383929.85727.qm@web125706.mail.ne1.yahoo.com> please remove from this mailing list --- On Thu, 4/7/11, ti fei wrote: From: ti fei Subject: [Histonet] Unsubscribe To: "Vanessa Avalos" , "HISTONET LISTS" Date: Thursday, April 7, 2011, 9:26 AM Hi please remove me from the mailing list ? ------------------ Original ------------------ ? From:? "Vanessa Avalos"; Date:? Thu, Apr 7, 2011 05:54 AM To:? "HISTONET LISTS"; Subject:? RE: [Histonet] Slides sent out for outside consult. ? Our practice does not bill the patient. Before sending they sign a waiver giving us permission to send out. On this waiver it states that if this is something their insurance does not cover then they may revieve a bill for $-$ range. We have an agreement with our consulting lab that they will only accept what the individual insurance covers. Our patients were getting ridiculous bills for consulting and our office was getting angry phone calls. After talking to the lab they made this agreement. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, April 06, 2011 2:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides sent out for outside consult. How is everyone handling a case that you send out to an outside consultant as far as billing the patient for the consult. Right now if I send out a case they bill me back an 88321. I am being told that I can not pass this same 88321 on to the patient. Having the outside consultant do the billing is not an option. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mollie <@t> phylogenyinc.com Wed Apr 20 19:21:18 2011 From: mollie <@t> phylogenyinc.com (Mollie Hannon) Date: Wed Apr 20 19:22:05 2011 Subject: [Histonet] Unsubscribe In-Reply-To: <383929.85727.qm@web125706.mail.ne1.yahoo.com> References: <383929.85727.qm@web125706.mail.ne1.yahoo.com> Message-ID: <81BCDB6F-2770-4C4B-833C-A55143E09806@phylogenyinc.com> Please remove me from this list. Thank you. On Apr 20, 2011, at 7:36 PM, Tate Maria wrote: > > please remove from this mailing list > --- On Thu, 4/7/11, ti fei wrote: > > From: ti fei > Subject: [Histonet] Unsubscribe > To: "Vanessa Avalos" , "HISTONET LISTS" > Date: Thursday, April 7, 2011, 9:26 AM > > Hi please remove me from the mailing list > ------------------ Original ------------------ > From: "Vanessa Avalos"; > Date: Thu, Apr 7, 2011 05:54 AM > To: "HISTONET LISTS"; > > Subject: RE: [Histonet] Slides sent out for outside consult. > > > Our practice does not bill the patient. Before sending they sign a waiver > giving us permission to send out. On this waiver it states that if this is > something their insurance does not cover then they may revieve a bill for > $-$ range. We have an agreement with our consulting lab that they will only > accept what the individual insurance covers. Our patients were getting > ridiculous bills for consulting and our office was getting angry phone > calls. After talking to the lab they made this agreement. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Wednesday, April 06, 2011 2:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slides sent out for outside consult. > > How is everyone handling a case that you send out to an outside > consultant as far as billing the patient for the consult. Right now if I > send out a case they bill me back an 88321. I am being told that I can > not pass this same 88321 on to the patient. Having the outside > consultant do the billing is not an option. > > Thanks, > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -----Inline Attachment Follows----- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmoore9k <@t> gmail.com Wed Apr 20 21:30:18 2011 From: tmoore9k <@t> gmail.com (Teresa Moore) Date: Wed Apr 20 21:30:21 2011 Subject: [Histonet] Graduating in June from histo program Message-ID: I will be graduating from the histology program at Columbus State Community College in June. Looking for a histotechnician position in or near Columbus, Ohio. Any advice or leads are appreciated. Thank you. Teresa Moore From A.Mortimer <@t> latrobe.edu.au Wed Apr 20 21:48:46 2011 From: A.Mortimer <@t> latrobe.edu.au (Abbey Mortimer) Date: Wed Apr 20 21:49:00 2011 Subject: [Histonet] RE: whole retinal staining In-Reply-To: <20110419164112.ANU62452@mstore03.uchicago.edu> References: <20110419164112.ANU62452@mstore03.uchicago.edu> Message-ID: Thank you all so very much! Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.mortimer@latrobe.edu.au ________________________________________ From: David A. Wright [dw18@uchicago.edu] Sent: Wednesday, 20 April 2011 7:41 AM To: histonet@lists.utsouthwestern.edu Cc: Abbey Mortimer Subject: whole retinal staining Dear Abbey & Histonet I agree with Ren? Buesa: I would try to stain whole retinae by a 'floating section' method i.e. hand processing in a dipping tray or mesh baskets (brush transfer can be done too but is laborious). Then mount them at the end. Floating allows reagents to penetrate from both sides of the retina - extra valuable because your incubation times are going to be very long with material this thick. (I assume you are not just interested in superficial layers.) A microwave processor would help with penetration, but I have no direct experience. -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery ORIGINAL POST: Histonet Digest, Vol 89, Issue 21 Message: 7 Date: Tue, 19 Apr 2011 19:00:27 +1000 From: Abbey Mortimer Subject: [Histonet] Tissue adhesion to slides Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.mortimer@latrobe.edu.au From lpwenk <@t> sbcglobal.net Thu Apr 21 04:48:41 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Apr 21 04:48:45 2011 Subject: [Histonet] How to Unsubscribe Message-ID: <1EA881612BC34D52BB4B1C32ADA42CCE@HP2010> It's a do-it-yourself. - Scroll to bottom of any Histonet email. - Click on the link that has the word "lists" in it. - Scroll to the bottom of that page, where there is information on how to unsubscribe. - Follow directions. Peggy Wenki From BMolinari <@t> heart.thi.tmc.edu Thu Apr 21 07:25:24 2011 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Apr 21 07:25:28 2011 Subject: [Histonet] trichrome/ frozen cardiac tissue Message-ID: Hi, I have some frozen house hearts that were perfused with 4% paraformaldehyde. I found protocols for FF, but not fixed frozen. I tried staining them with my usual Gomori I use on my paraffin blocks but the doc thinks they picked up too much red, not enough green. Any suggerstions..I need to get them done today, they were brought up to RT this morning. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From jshelley <@t> sanfordburnham.org Thu Apr 21 08:13:39 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Thu Apr 21 08:13:45 2011 Subject: [Histonet] UCP1 Antibody Message-ID: Hi Histonetters, I was hoping those of you who may have worked with UCP1 antibody might be willing to share how you optimized this antibody. I am using UCP1 antibody (ab10983) from Abcam. I have followed the datasheet and have used both protease and proteinase k and at varying times with no results. I am using brown fat from a wild type mouse and this is the control tissue that should work. I am running it overnight in a 4 ? to see if that might help since the Post Doc said that their western blot worked better that way. Any help or ideas how to proceed would be appreciated. Thanks in advance! Kind Regards! John J Shelley From Ashley.Troutman <@t> Vanderbilt.Edu Thu Apr 21 08:16:39 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Apr 21 08:16:44 2011 Subject: [Histonet] IHC validation Message-ID: <7B310892042DA74CB3590053F424CFE613D63CD2A5@ITS-HCWNEM06.ds.Vanderbilt.edu> Hematoxylin should not affect the immunoreactivity (since counterstaining occurs following the IHC reaction), so no, changing the hematoxylin would not require a revalidation. Just make sure you are not obscuring the immunostain with an overly-dark counterstain. The point is to provide contrast between the DAB (brown) or fast red chromagen and to offer the reader a sense of context of the surrounding morphology of the tissue being stained. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 10 Date: Tue, 19 Apr 2011 14:04:32 -0500 From: "Ring, Mary L" Subject: [Histonet] IHC validation To: "histonet@lists.utsouthwestern.edu" Message-ID: <2594D39B69223047B6E2F4ADF1E62DB9010FFB487841@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="iso-8859-1" Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn From cpyse <@t> x-celllab.com Thu Apr 21 08:51:35 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Thu Apr 21 08:52:46 2011 Subject: [Histonet] cyto prep Message-ID: <001501cc002b$3a929360$afb7ba20$@com> Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cpyse@x-celllab.com From cbrya <@t> lexclin.com Thu Apr 21 09:10:02 2011 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Apr 21 09:10:06 2011 Subject: [Histonet] processing times for skin specimens Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05C77A9C7F@EXCHANGESB> Hello everyone! What is the shortest cycle you may be using for processing skin specimens? Can skin biopsies, shaves, and punches be processed well on a short run on the VIP 6? I need to find a successful protocol if possible to run skins on a 6 hour process, if possible. Thank you in advance for any input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From Ronald.Houston <@t> nationwidechildrens.org Thu Apr 21 09:00:22 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Apr 21 09:12:46 2011 Subject: [Histonet] IHC menu Message-ID: Would anyone be willing to share their IHC menu with us? I want to see how we compare with other top-class facilities. Obviously I am more interested in pediatric facilities, but we are constantly evaluating antibodies for research studies, and what may have applications in pediatric pathology from the adult world. I'd be happy to send our menu on return Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From micropathlabs <@t> yahoo.com Thu Apr 21 09:23:36 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Apr 21 09:23:39 2011 Subject: [Histonet] cyto prep In-Reply-To: <001501cc002b$3a929360$afb7ba20$@com> References: <001501cc002b$3a929360$afb7ba20$@com> Message-ID: <104166.87242.qm@web161712.mail.bf1.yahoo.com> We have lab assistants running thin preps. Our cytotechs screen and do HPV. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? ________________________________ From: Cynthia Pyse To: Histonet Sent: Thu, April 21, 2011 9:51:35 AM Subject: [Histonet] cyto prep Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Apr 21 09:25:03 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Apr 21 09:26:32 2011 Subject: [Histonet] Unsubscribe In-Reply-To: <81BCDB6F-2770-4C4B-833C-A55143E09806@phylogenyinc.com> References: <383929.85727.qm@web125706.mail.ne1.yahoo.com> <81BCDB6F-2770-4C4B-833C-A55143E09806@phylogenyinc.com> Message-ID: Jupiter's thunder!!!! two people in a row? really?!!?!? LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT YOU SUBSCRIBED ON. Both of you are officially banned from the internet. Forever. Don't use it again. And don't EVER join a mailing list. EVER. Emily, who would love to unsubscribe you so I didn't have to get your ridiculous emails about how you can't do it your damn self. A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron From JWeems <@t> sjha.org Thu Apr 21 09:31:13 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Apr 21 09:31:18 2011 Subject: [Histonet] cyto prep In-Reply-To: <001501cc002b$3a929360$afb7ba20$@com> References: <001501cc002b$3a929360$afb7ba20$@com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F4BBA4D@CHEXCMS10.one.ads.che.org> We no longer do Gyn, but when we did we had lab assistants do the prep. Have lab asst for non-Gyn prep currently. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, April 21, 2011 09:52 To: 'Histonet' Subject: [Histonet] cyto prep Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From leiker <@t> buffalo.edu Thu Apr 21 09:34:44 2011 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Apr 21 09:35:06 2011 Subject: [Histonet] Unsubscribe In-Reply-To: References: <383929.85727.qm@web125706.mail.ne1.yahoo.com> <81BCDB6F-2770-4C4B-833C-A55143E09806@phylogenyinc.com> Message-ID: <91F17A8EA5A1AFDA3FBCAB34@CDYwxp1931.ad.med.buffalo.edu> **Listserv master: Some people are reporting problems with managing their subscription as they have submitted their unsubscription multiple times in the prescribed manner and are still getting Histonet emails. This was reported to me by one such (failed) unsubscriber.** --On Thursday, April 21, 2011 10:25 AM -0400 Emily Sours wrote: > Jupiter's thunder!!!! > two people in a row? really?!!?!? > LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT > YOU SUBSCRIBED ON. > > Both of you are officially banned from the internet. Forever. Don't use > it again. > And don't EVER join a mailing list. EVER. > > Emily, who would love to unsubscribe you so I didn't have to get your > ridiculous emails about how you can't do it your damn self. > > A great book should leave you with many experiences, and slightly > exhausted. You should live several lives while reading it. > -William Styron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6118 Fx: (716) 829-2665 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From christi.cosby <@t> gmail.com Thu Apr 21 11:18:23 2011 From: christi.cosby <@t> gmail.com (Christi Cosby) Date: Thu Apr 21 11:18:28 2011 Subject: [Histonet] eosin bleeding on frozen sections Message-ID: Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid H&E stain on frozen sections. Any advice would be greatly appreciated! From aevans3 <@t> lghealth.org Thu Apr 21 11:19:14 2011 From: aevans3 <@t> lghealth.org (Evans, Andria B) Date: Thu Apr 21 11:20:21 2011 Subject: [Histonet] Neutralizing Formalin Message-ID: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA65@MAIL-AG-CLUSTER.lha.org> Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!! Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sgoebel <@t> mirnarx.com Thu Apr 21 11:26:56 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Apr 21 11:27:00 2011 Subject: [Histonet] eosin bleeding on frozen sections In-Reply-To: References: Message-ID: This happened to me a couple weeks ago. You got water on the slide after the eosin staining. Just run it back through alcohols, and rinse for about five minutes. Change out the alcohols, and re-stain. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christi Cosby Sent: Thursday, April 21, 2011 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin bleeding on frozen sections Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid H&E stain on frozen sections. Any advice would be greatly appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nelsonrnch <@t> verizon.net Thu Apr 21 11:36:46 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Thu Apr 21 11:36:50 2011 Subject: [Histonet] (no subject) Message-ID: <193223.55791.qm@web84301.mail.re1.yahoo.com> Hi from Southern Cali, We neutralize our formalin with a product called Neutralex. It comes in powder form and after?30 mins we can dump it down the drain. We also keep records?for?QC purposes.?Product is?purchased from a company called American MasterTech. Hope this gives you a starting place. ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net From nancy_schmitt <@t> pa-ucl.com Thu Apr 21 12:05:22 2011 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Apr 21 12:05:30 2011 Subject: [Histonet] cyto prep In-Reply-To: <20110421164329.4CF0419198C@mail.pa-ucl.com> References: <20110421164329.4CF0419198C@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC06445691FD6F6@hestia.ad.pa-ucl.com> Hi Cindy- We have cyto prep/lab asst. position that performs pap and non-gyn preparation. Cytotechs screen and HPVs are sent out. Nancy Schmitt HT, MLT(ASCP) Dubuque, IA Date: Thu, 21 Apr 2011 09:51:35 -0400 From: "Cynthia Pyse" Subject: [Histonet] cyto prep To: "'Histonet'" Message-ID: <001501cc002b$3a929360$afb7ba20$@com> Content-Type: text/plain; charset="us-ascii" Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cpyse@x-celllab.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From laurie.colbert <@t> huntingtonhospital.com Thu Apr 21 12:18:11 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Apr 21 12:18:14 2011 Subject: [Histonet] eosin bleeding on frozen sections In-Reply-To: References: Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AB82@EXCHANGE3.huntingtonhospital.com> Try dehydrating longer. If there is water left on the slide, the eosin will bleed. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christi Cosby Sent: Thursday, April 21, 2011 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin bleeding on frozen sections Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid H&E stain on frozen sections. Any advice would be greatly appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Apr 21 12:59:10 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Apr 21 12:59:14 2011 Subject: [Histonet] decalcification of articular cartilage In-Reply-To: Message-ID: We do a lot of IHC staining on bone and cartilage samples and we use 10% formic acid for decalcification for immunohistochemistry. I'm afraid you may not be able to turnaround this in a week. The tissue needs to be adequately fixed prior to decalcification, and properly decaled. I would trim the tissue to about 4 mm thick if you can on a saw you might be able to turn this around in your time frame, but I have learned from experience if you rush decalcification you have a greater chance of running into problems. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz Sent: Wednesday, April 20, 2011 8:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of articular cartilage Dear Members, We're planning to study sheep knee articular cartilage with histochemistry and immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde at the moment. We need an effective, quick and cartilage tissue protective decalcification method. We'll perform only light microscopic examination in this study and we have to complete the study within one week. In these circumstances what are your advices? Thanks in advance. Dr. Necat Yilmaz University of Mersin School of Medicine __________ ESET NOD32 Antivirus Ak?ll? G?venlik taraf?ndan sa?lanan bilgiler, vir?s imza veritaban? s?r?m?: 6057 (20110420) __________ ?leti ESET NOD32 Antivirus Ak?ll? G?venlik taraf?ndan denetlendi. http://www.nod32.com.tr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Thu Apr 21 13:07:17 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Apr 21 13:07:20 2011 Subject: [Histonet] decalcification of articular cartilage In-Reply-To: References: Message-ID: You could always get formical which fixes and decals at the same time. This would eliminate the waiting for fixation. Although I do usually let it fix in straight formalin overnight first. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, April 21, 2011 12:59 PM To: Nejat Yilmaz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decalcification of articular cartilage We do a lot of IHC staining on bone and cartilage samples and we use 10% formic acid for decalcification for immunohistochemistry. I'm afraid you may not be able to turnaround this in a week. The tissue needs to be adequately fixed prior to decalcification, and properly decaled. I would trim the tissue to about 4 mm thick if you can on a saw you might be able to turn this around in your time frame, but I have learned from experience if you rush decalcification you have a greater chance of running into problems. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz Sent: Wednesday, April 20, 2011 8:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of articular cartilage Dear Members, We're planning to study sheep knee articular cartilage with histochemistry and immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde at the moment. We need an effective, quick and cartilage tissue protective decalcification method. We'll perform only light microscopic examination in this study and we have to complete the study within one week. In these circumstances what are your advices? Thanks in advance. Dr. Necat Yilmaz University of Mersin School of Medicine __________ ESET NOD32 Antivirus Ak?ll? G?venlik taraf?ndan sa?lanan bilgiler, vir?s imza veritaban? s?r?m?: 6057 (20110420) __________ ?leti ESET NOD32 Antivirus Ak?ll? G?venlik taraf?ndan denetlendi. http://www.nod32.com.tr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nguy0515 <@t> gmail.com Thu Apr 21 13:10:43 2011 From: nguy0515 <@t> gmail.com (Trini Nguyen) Date: Thu Apr 21 13:10:47 2011 Subject: [Histonet] question on Leica ST4020 stainer users Message-ID: Hello, Does anyone use the Leica St4020 stainer? If so, how do you have the stainer set up and how many slides do you run on the stainer before you change reagents? Which reagents do you change and which do you not? Thanks, Trini From baier.patricia <@t> marshfieldclinic.org Thu Apr 21 13:46:25 2011 From: baier.patricia <@t> marshfieldclinic.org (Baier, Patricia) Date: Thu Apr 21 13:46:29 2011 Subject: [Histonet] (no subject) Message-ID: <201104211846.p3LIkQA4004346@mailhost2.mfldclin.edu> We are currently in need of some ergonomic chairs for our embedding and microtomy stations. Does anyone have any informatio to share in reguard to chairs? ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From Clough <@t> medicine.tamhsc.edu Thu Apr 21 14:07:54 2011 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Thu Apr 21 14:09:27 2011 Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. Message-ID: While sectioning paraffin embedded tissue on a rotary microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise for me. Thanks in advance for your suggestions . Bret From sbreeden <@t> nmda.nmsu.edu Thu Apr 21 14:10:20 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Apr 21 14:10:24 2011 Subject: [Histonet] (no subject) In-Reply-To: <201104211846.p3LIkQA4004346@mailhost2.mfldclin.edu> References: <201104211846.p3LIkQA4004346@mailhost2.mfldclin.edu> Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47820@nmdamailsvr.nmda.ad.nmsu.edu> Yes - make sure they're long enough in the SEAT! Mine roll around the lab like little 5-wheeled rockets, but cut off the circulation to my thighs (is that something I can say on television?). And no arms on the chairs! From liz <@t> premierlab.com Thu Apr 21 14:13:59 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Apr 21 14:14:03 2011 Subject: [Histonet] processing times for skin specimens In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF05C77A9C7F@EXCHANGESB> Message-ID: Carol We are a research lab but we have always processed our skin samples on longer processing cycles, even the punch biopsy samples. The shaves might be different, if I wanted to cut down I would start with how you normally process them and then cut down at 5 minute increments to start and see how that goes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, April 21, 2011 8:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] processing times for skin specimens Hello everyone! What is the shortest cycle you may be using for processing skin specimens? Can skin biopsies, shaves, and punches be processed well on a short run on the VIP 6? I need to find a successful protocol if possible to run skins on a 6 hour process, if possible. Thank you in advance for any input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 21 14:26:18 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 21 14:26:21 2011 Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. In-Reply-To: Message-ID: <631746.44415.qm@web65716.mail.ac4.yahoo.com> 1- change cutting angle 2- keep the blade holder clean (without paraffin) 3- make sure the blade is not blunt Ren? J. ? ? --- On Thu, 4/21/11, Clough, Bret wrote: From: Clough, Bret Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. To: "Histonet list serv." Date: Thursday, April 21, 2011, 3:07 PM While sectioning paraffin embedded tissue on a rotary? microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise? for me. Thanks in advance for your suggestions . Bret _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu Apr 21 15:04:41 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Apr 21 15:04:44 2011 Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. In-Reply-To: <631746.44415.qm@web65716.mail.ac4.yahoo.com> References: <631746.44415.qm@web65716.mail.ac4.yahoo.com> Message-ID: Keep everything cold, cold, cold. The block, the blade, the blade holder. Cold spray the blade and blade holder, cool the block on ice. Warning: Use cold spray sparingly on the block, you can ruin your tissue with too much. Hullabaloo, Caneck! Caneck! Jay A. Lundgren, M.S., HTL (ASCP) From talulahgosh <@t> gmail.com Thu Apr 21 15:27:10 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Apr 21 15:27:14 2011 Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. In-Reply-To: References: Message-ID: Open a beer to the science gods. It may also help to follow the other instructions sent to the histonet list. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Thu, Apr 21, 2011 at 3:07 PM, Clough, Bret wrote: > While sectioning paraffin embedded tissue on a rotary microtome I noticed > the sections sticking to the blade holder and bunching up. What do I need to > do so that my section come off the blade in a ribbon? Please note that this > is my first time in sectioning tissue and this is a learning exercise for > me. > > Thanks in advance for your suggestions . > > Bret > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Thu Apr 21 15:32:43 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Apr 21 15:32:47 2011 Subject: [Histonet] endogenous peroxidases Message-ID: Hello I know I can block endogenous peroxidases in paraffin sections before antigen retrieval or after it, but which is better? Does it make a difference? Also, since our H2O2 is 30%, do I need to account for the water in it when I'm diluting it in 100% MeOH? (I was going to use a 0.3% H2O2 in MeOH wash for 30 minutes) I'm not even sure how I would account for it, I just thought it might affect the MeOH since it wouldn't be anhydrous anymore. Emily ps put your hand to the screen for an internet high five!! it's national high five day! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron From rjbuesa <@t> yahoo.com Thu Apr 21 15:58:32 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 21 15:58:36 2011 Subject: [Histonet] endogenous peroxidases In-Reply-To: Message-ID: <681015.91692.qm@web65715.mail.ac4.yahoo.com> The endogenous peroxidase has to be blocked (quenching others call this step) only before the procedure because the idea is to avoid the interference of the peroxidase with it. Ren? J. --- On Thu, 4/21/11, Emily Sours wrote: From: Emily Sours Subject: [Histonet] endogenous peroxidases To: histonet@lists.utsouthwestern.edu Date: Thursday, April 21, 2011, 4:32 PM Hello I know I can block endogenous peroxidases in paraffin sections before antigen retrieval or after it, but which is better? Does it make a difference? Also, since our H2O2 is 30%, do I need to account for the water in it when I'm diluting it in 100% MeOH? (I was going to use a 0.3% H2O2 in MeOH wash for 30 minutes) I'm not even sure how I would account for it, I just thought it might affect the MeOH since it wouldn't be anhydrous anymore. Emily ps put your hand to the screen for an internet high five!! it's national high five day! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtank2001 <@t> hotmail.com Thu Apr 21 16:21:33 2011 From: mtank2001 <@t> hotmail.com (Martino T.) Date: Thu Apr 21 16:21:36 2011 Subject: [Histonet] Mohs Key Performane Indicators (Recuts) Message-ID: Hello, I work for a Mohs surgeon who wants to establish key performance indicators (KPIs) for the mohs techs to evaluate daily performance. Does anyone have any information of surveys or standards for the number of recuts per day or per block as a measure for quality Mohs sectioning? She's working with a standard that 4 or less recuts in a day (average 30 to 50 blocks per day) is quality work. Has anyone come up with standards in this regard for Mohs sectioning? Thanks in advance, Martino Tanner ?Life can either be accepted or changed. If it is not accepted, it must be changed. If it cannot be changed, then it must be accepted.? From mhunt312 <@t> earthlink.net Thu Apr 21 17:06:19 2011 From: mhunt312 <@t> earthlink.net (Mary) Date: Thu Apr 21 17:06:28 2011 Subject: [Histonet] Input needed Message-ID: What?s the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason From JMacDonald <@t> mtsac.edu Thu Apr 21 18:00:52 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Apr 21 18:21:19 2011 Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. In-Reply-To: Message-ID: Another culprit is static. Moisture helps to keep the static down. "Clough, Bret" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/21/2011 12:11 PM To Histonet list serv. cc Subject [Histonet] How to keep paraffin sections from sticking to blade/blade holder. While sectioning paraffin embedded tissue on a rotary microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise for me. Thanks in advance for your suggestions . Bret _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Apr 21 18:09:44 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Apr 21 18:21:21 2011 Subject: [Histonet] Input needed In-Reply-To: Message-ID: I have had graduates from my program that are "mature". They have not had problems getting jobs, or advancing once hired. That is true for either gender. A good tech is a good tech. Jennifer MacDonald Mt. San Antonio College "Mary" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/21/2011 03:08 PM To cc Subject [Histonet] Input needed What?s the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM <@t> mcclainlab.com Thu Apr 21 19:24:29 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Thu Apr 21 19:13:42 2011 Subject: [Histonet] processing times for skin specimens In-Reply-To: References: Message-ID: 90 minutes for up to 2mm thickness using K3000 or VIP5. I do not have a VIP6 Steve A. McClain, MD McClain Laboratories, LLC 45 Manor Road Smithtown, NY 11787 631 361-4000 631 361-4037fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, April 21, 2011 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 89, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Cassette labeler (Martin, Gary) 2. 'Deplasticizing' before staining? (Mahesh Polavarapu) 3. Re: Request help on finding a part for Leitz 1512 (Henry Chidgey) 4. RELIA Histology Job Alert. Excellent opportunity on Long Island Day shift NY Lic required. (Pam Barker) 5. RE: Re: Request help on finding a part for Leitz 1512 (Weems, Joyce) 6. Re: Re: Request help on finding a part for Leitz 1512 (Grantham, Andrea L - (algranth)) 7. Part time Histo Tech position in Phoenix AZ (jcox90@yahoo.com) 8. Unsubscribe (Tate Maria) 9. Re: Unsubscribe (Mollie Hannon) 10. Graduating in June from histo program (Teresa Moore) 11. RE: whole retinal staining (Abbey Mortimer) 12. How to Unsubscribe (Lee & Peggy Wenk) 13. trichrome/ frozen cardiac tissue (Molinari, Betsy) 14. UCP1 Antibody (John Shelley) 15. IHC validation (Troutman, Kenneth A) 16. cyto prep (Cynthia Pyse) 17. processing times for skin specimens (Carol Bryant) 18. IHC menu (Houston, Ronald) 19. Re: cyto prep (Sheila Haas) 20. Re: Unsubscribe (Emily Sours) 21. RE: cyto prep (Weems, Joyce) 22. Re: Unsubscribe (Merced Leiker) 23. eosin bleeding on frozen sections (Christi Cosby) 24. Neutralizing Formalin (Evans, Andria B) 25. RE: eosin bleeding on frozen sections (sgoebel@mirnarx.com) 26. (no subject) (SHANE NELSON) ---------------------------------------------------------------------- Message: 1 Date: Wed, 20 Apr 2011 10:12:34 -0700 From: "Martin, Gary" Subject: [Histonet] Cassette labeler To: Message-ID: <6ED9D4252F278841A0593D3D788AF24C0D480110@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" We have just received a Lamb Micro Writer (model # E22.01MWI) single shoe. The problem is that we did not receive an operator manual. Does anyone know where I can get a manual? ------------------------------ Message: 2 Date: Wed, 20 Apr 2011 12:24:55 -0500 From: Mahesh Polavarapu Subject: [Histonet] 'Deplasticizing' before staining? To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I have 50uM thick sections of soft tissue embedded in MMA (cold cured Technovit 9100). Do these need to be 'deplasticized' before they will take up stain? Thanks in advance! - Mahesh ------------------------------ Message: 3 Date: Wed, 20 Apr 2011 12:31:10 -0500 From: "Henry Chidgey" Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 To: Message-ID: <016f01cbff80$bef19030$3cd4b090$@net> Content-Type: text/plain; charset="us-ascii" I was asked by a member to share the solution so here it is : Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. He will probably have some or can make you some. Great guy and very knowledgeable. Thanks Joyce for giving me this informationJ Henry ------------------------------ Message: 4 Date: Wed, 20 Apr 2011 13:36:45 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Histology Job Alert. Excellent opportunity on Long Island Day shift NY Lic required. To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! I have a great new opportunity that I want to tell you about. I am working with a Laboratory located on Long Island that is in need of a NY licensed histology tech. This is a dayshift full time permanent position. My client offers a very competitive salary, excellent benefits, a great place to work and they are ready to interview and hire right away. If you or anyone you know might be interested in this position please contact me. I can be reached toll free at 866-607-3542 or relia1@earthlink.net. Thanks and have a great day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 5 Date: Wed, 20 Apr 2011 14:54:58 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Re: Request help on finding a part for Leitz 1512 To: "hchidgey@earthlink.net" , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F4BB962@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" He worked in the Leica factory and is truly an expert. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Henry Chidgey Sent: Wednesday, April 20, 2011 13:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 I was asked by a member to share the solution so here it is : Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. He will probably have some or can make you some. Great guy and very knowledgeable. Thanks Joyce for giving me this informationJ Henry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 6 Date: Wed, 20 Apr 2011 12:51:48 -0700 From: "Grantham, Andrea L - (algranth)" Subject: Re: [Histonet] Re: Request help on finding a part for Leitz 1512 To: "hchidgey@earthlink.net" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I second getting in touch with Klaus - he knows the 1512 inside and out. Andi Grantham On Apr 20, 2011, at 10:31 AM, Henry Chidgey wrote: > I was asked by a member to share the solution so here it is : > > Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. > He will probably have some or can make you some. > > Great guy and very knowledgeable. Thanks Joyce for giving me this > informationJ > > Henry > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Wed, 20 Apr 2011 13:08:11 -0700 (PDT) From: jcox90@yahoo.com Subject: [Histonet] Part time Histo Tech position in Phoenix AZ To: "Histonet@Lists. Utsouthwestern. Edu" Message-ID: <58759.56388.qm@web161609.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Part time Histo Tech position open with Southwest Skin Specialists, Ltd., located in Phoenix AZ.? Responsibilities for this position include: grossing and fixation, processing, embedding, sectioning and staining.??? Education Requirements: ?Associate Degree in Applied Science/Histology and/or Histologic Certificate and HT or HTL ASCP Certification.? If interested please send resume to miverson@swskin.net. ? Jill Cox, HT ASCP ------------------------------ Message: 8 Date: Wed, 20 Apr 2011 16:36:01 -0700 (PDT) From: Tate Maria Subject: [Histonet] Unsubscribe To: histonet@lists.utsouthwestern.edu Message-ID: <383929.85727.qm@web125706.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 please remove from this mailing list --- On Thu, 4/7/11, ti fei wrote: From: ti fei Subject: [Histonet] Unsubscribe To: "Vanessa Avalos" , "HISTONET LISTS" Date: Thursday, April 7, 2011, 9:26 AM Hi please remove me from the mailing list ? ------------------ Original ------------------ ? From:? "Vanessa Avalos"; Date:? Thu, Apr 7, 2011 05:54 AM To:? "HISTONET LISTS"; Subject:? RE: [Histonet] Slides sent out for outside consult. ? Our practice does not bill the patient. Before sending they sign a waiver giving us permission to send out. On this waiver it states that if this is something their insurance does not cover then they may revieve a bill for $-$ range. We have an agreement with our consulting lab that they will only accept what the individual insurance covers. Our patients were getting ridiculous bills for consulting and our office was getting angry phone calls. After talking to the lab they made this agreement. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, April 06, 2011 2:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides sent out for outside consult. How is everyone handling a case that you send out to an outside consultant as far as billing the patient for the consult. Right now if I send out a case they bill me back an 88321. I am being told that I can not pass this same 88321 on to the patient. Having the outside consultant do the billing is not an option. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 20 Apr 2011 20:21:18 -0400 From: Mollie Hannon Subject: Re: [Histonet] Unsubscribe To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <81BCDB6F-2770-4C4B-833C-A55143E09806@phylogenyinc.com> Content-Type: text/plain; charset=us-ascii Please remove me from this list. Thank you. On Apr 20, 2011, at 7:36 PM, Tate Maria wrote: > > please remove from this mailing list > --- On Thu, 4/7/11, ti fei wrote: > > From: ti fei > Subject: [Histonet] Unsubscribe > To: "Vanessa Avalos" , "HISTONET LISTS" > Date: Thursday, April 7, 2011, 9:26 AM > > Hi please remove me from the mailing list > ------------------ Original ------------------ > From: "Vanessa Avalos"; > Date: Thu, Apr 7, 2011 05:54 AM > To: "HISTONET LISTS"; > > Subject: RE: [Histonet] Slides sent out for outside consult. > > > Our practice does not bill the patient. Before sending they sign a waiver > giving us permission to send out. On this waiver it states that if this is > something their insurance does not cover then they may revieve a bill for > $-$ range. We have an agreement with our consulting lab that they will only > accept what the individual insurance covers. Our patients were getting > ridiculous bills for consulting and our office was getting angry phone > calls. After talking to the lab they made this agreement. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Wednesday, April 06, 2011 2:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slides sent out for outside consult. > > How is everyone handling a case that you send out to an outside > consultant as far as billing the patient for the consult. Right now if I > send out a case they bill me back an 88321. I am being told that I can > not pass this same 88321 on to the patient. Having the outside > consultant do the billing is not an option. > > Thanks, > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -----Inline Attachment Follows----- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 20 Apr 2011 22:30:18 -0400 From: Teresa Moore Subject: [Histonet] Graduating in June from histo program To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I will be graduating from the histology program at Columbus State Community College in June. Looking for a histotechnician position in or near Columbus, Ohio. Any advice or leads are appreciated. Thank you. Teresa Moore ------------------------------ Message: 11 Date: Thu, 21 Apr 2011 12:48:46 +1000 From: Abbey Mortimer Subject: [Histonet] RE: whole retinal staining To: "David A. Wright" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Thank you all so very much! Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.mortimer@latrobe.edu.au ________________________________________ From: David A. Wright [dw18@uchicago.edu] Sent: Wednesday, 20 April 2011 7:41 AM To: histonet@lists.utsouthwestern.edu Cc: Abbey Mortimer Subject: whole retinal staining Dear Abbey & Histonet I agree with Ren? Buesa: I would try to stain whole retinae by a 'floating section' method i.e. hand processing in a dipping tray or mesh baskets (brush transfer can be done too but is laborious). Then mount them at the end. Floating allows reagents to penetrate from both sides of the retina - extra valuable because your incubation times are going to be very long with material this thick. (I assume you are not just interested in superficial layers.) A microwave processor would help with penetration, but I have no direct experience. -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery ORIGINAL POST: Histonet Digest, Vol 89, Issue 21 Message: 7 Date: Tue, 19 Apr 2011 19:00:27 +1000 From: Abbey Mortimer Subject: [Histonet] Tissue adhesion to slides Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.mortimer@latrobe.edu.au ------------------------------ Message: 12 Date: Thu, 21 Apr 2011 05:48:41 -0400 From: "Lee & Peggy Wenk" Subject: [Histonet] How to Unsubscribe To: "Histonet" Message-ID: <1EA881612BC34D52BB4B1C32ADA42CCE@HP2010> Content-Type: text/plain; charset="iso-8859-1" It's a do-it-yourself. - Scroll to bottom of any Histonet email. - Click on the link that has the word "lists" in it. - Scroll to the bottom of that page, where there is information on how to unsubscribe. - Follow directions. Peggy Wenki ------------------------------ Message: 13 Date: Thu, 21 Apr 2011 12:25:24 +0000 From: "Molinari, Betsy" Subject: [Histonet] trichrome/ frozen cardiac tissue To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I have some frozen house hearts that were perfused with 4% paraformaldehyde. I found protocols for FF, but not fixed frozen. I tried staining them with my usual Gomori I use on my paraffin blocks but the doc thinks they picked up too much red, not enough green. Any suggerstions..I need to get them done today, they were brought up to RT this morning. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ------------------------------ Message: 14 Date: Thu, 21 Apr 2011 09:13:39 -0400 From: John Shelley Subject: [Histonet] UCP1 Antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, I was hoping those of you who may have worked with UCP1 antibody might be willing to share how you optimized this antibody. I am using UCP1 antibody (ab10983) from Abcam. I have followed the datasheet and have used both protease and proteinase k and at varying times with no results. I am using brown fat from a wild type mouse and this is the control tissue that should work. I am running it overnight in a 4 ? to see if that might help since the Post Doc said that their western blot worked better that way. Any help or ideas how to proceed would be appreciated. Thanks in advance! Kind Regards! John J Shelley ------------------------------ Message: 15 Date: Thu, 21 Apr 2011 08:16:39 -0500 From: "Troutman, Kenneth A" Subject: [Histonet] IHC validation To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7B310892042DA74CB3590053F424CFE613D63CD2A5@ITS-HCWNEM06.ds.Vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" Hematoxylin should not affect the immunoreactivity (since counterstaining occurs following the IHC reaction), so no, changing the hematoxylin would not require a revalidation. Just make sure you are not obscuring the immunostain with an overly-dark counterstain. The point is to provide contrast between the DAB (brown) or fast red chromagen and to offer the reader a sense of context of the surrounding morphology of the tissue being stained. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 10 Date: Tue, 19 Apr 2011 14:04:32 -0500 From: "Ring, Mary L" Subject: [Histonet] IHC validation To: "histonet@lists.utsouthwestern.edu" Message-ID: <2594D39B69223047B6E2F4ADF1E62DB9010FFB487841@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="iso-8859-1" Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn ------------------------------ Message: 16 Date: Thu, 21 Apr 2011 09:51:35 -0400 From: "Cynthia Pyse" Subject: [Histonet] cyto prep To: "'Histonet'" Message-ID: <001501cc002b$3a929360$afb7ba20$@com> Content-Type: text/plain; charset="us-ascii" Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cpyse@x-celllab.com ------------------------------ Message: 17 Date: Thu, 21 Apr 2011 10:10:02 -0400 From: Carol Bryant Subject: [Histonet] processing times for skin specimens To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05C77A9C7F@EXCHANGESB> Content-Type: text/plain; charset="us-ascii" Hello everyone! What is the shortest cycle you may be using for processing skin specimens? Can skin biopsies, shaves, and punches be processed well on a short run on the VIP 6? I need to find a successful protocol if possible to run skins on a 6 hour process, if possible. Thank you in advance for any input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ------------------------------ Message: 18 Date: Thu, 21 Apr 2011 10:00:22 -0400 From: "Houston, Ronald" Subject: [Histonet] IHC menu To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Would anyone be willing to share their IHC menu with us? I want to see how we compare with other top-class facilities. Obviously I am more interested in pediatric facilities, but we are constantly evaluating antibodies for research studies, and what may have applications in pediatric pathology from the adult world. I'd be happy to send our menu on return Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 19 Date: Thu, 21 Apr 2011 07:23:36 -0700 (PDT) From: Sheila Haas Subject: Re: [Histonet] cyto prep To: Cynthia Pyse , Histonet Message-ID: <104166.87242.qm@web161712.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We have lab assistants running thin preps. Our cytotechs screen and do HPV. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? ________________________________ From: Cynthia Pyse To: Histonet Sent: Thu, April 21, 2011 9:51:35 AM Subject: [Histonet] cyto prep Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 21 Apr 2011 10:25:03 -0400 From: Emily Sours Subject: Re: [Histonet] Unsubscribe To: Mollie Hannon , spanish_fly1981@yahoo.com Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Jupiter's thunder!!!! two people in a row? really?!!?!? LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT YOU SUBSCRIBED ON. Both of you are officially banned from the internet. Forever. Don't use it again. And don't EVER join a mailing list. EVER. Emily, who would love to unsubscribe you so I didn't have to get your ridiculous emails about how you can't do it your damn self. A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ------------------------------ Message: 21 Date: Thu, 21 Apr 2011 10:31:13 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] cyto prep To: Cynthia Pyse , 'Histonet' Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F4BBA4D@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We no longer do Gyn, but when we did we had lab assistants do the prep. Have lab asst for non-Gyn prep currently. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, April 21, 2011 09:52 To: 'Histonet' Subject: [Histonet] cyto prep Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 22 Date: Thu, 21 Apr 2011 10:34:44 -0400 From: Merced Leiker Subject: Re: [Histonet] Unsubscribe To: Emily Sours , Mollie Hannon , spanish_fly1981@yahoo.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <91F17A8EA5A1AFDA3FBCAB34@CDYwxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed **Listserv master: Some people are reporting problems with managing their subscription as they have submitted their unsubscription multiple times in the prescribed manner and are still getting Histonet emails. This was reported to me by one such (failed) unsubscriber.** --On Thursday, April 21, 2011 10:25 AM -0400 Emily Sours wrote: > Jupiter's thunder!!!! > two people in a row? really?!!?!? > LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT > YOU SUBSCRIBED ON. > > Both of you are officially banned from the internet. Forever. Don't use > it again. > And don't EVER join a mailing list. EVER. > > Emily, who would love to unsubscribe you so I didn't have to get your > ridiculous emails about how you can't do it your damn self. > > A great book should leave you with many experiences, and slightly > exhausted. You should live several lives while reading it. > -William Styron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6118 Fx: (716) 829-2665 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 23 Date: Thu, 21 Apr 2011 11:18:23 -0500 From: Christi Cosby Subject: [Histonet] eosin bleeding on frozen sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid H&E stain on frozen sections. Any advice would be greatly appreciated! ------------------------------ Message: 24 Date: Thu, 21 Apr 2011 12:19:14 -0400 From: "Evans, Andria B" Subject: [Histonet] Neutralizing Formalin To: "histonet@lists.utsouthwestern.edu" Message-ID: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA65@MAIL-AG-CLUSTER.lha.org> Content-Type: text/plain; charset="iso-8859-1" Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!! Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 25 Date: Thu, 21 Apr 2011 11:26:56 -0500 From: Subject: RE: [Histonet] eosin bleeding on frozen sections To: , Message-ID: Content-Type: text/plain; charset="us-ascii" This happened to me a couple weeks ago. You got water on the slide after the eosin staining. Just run it back through alcohols, and rinse for about five minutes. Change out the alcohols, and re-stain. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christi Cosby Sent: Thursday, April 21, 2011 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin bleeding on frozen sections Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid H&E stain on frozen sections. Any advice would be greatly appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Thu, 21 Apr 2011 09:36:46 -0700 (PDT) From: SHANE NELSON Subject: [Histonet] (no subject) To: Histonet@lists.utsouthwestern.edu Message-ID: <193223.55791.qm@web84301.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi from Southern Cali, We neutralize our formalin with a product called Neutralex. It comes in powder form and after?30 mins we can dump it down the drain. We also keep records?for?QC purposes.?Product is?purchased from a company called American MasterTech. Hope this gives you a starting place. ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 89, Issue 24 **************************************** From njblademaster <@t> gmail.com Thu Apr 21 20:45:54 2011 From: njblademaster <@t> gmail.com (Nathan Jentsch) Date: Thu Apr 21 20:46:19 2011 Subject: [Histonet] Re: processing times for skin specimens Message-ID: We do a short run some mornings for shaves and small punches. Keep in mind that we don't work 24/7, so these have been sitting in formalin overnight. 20 min formalin 1 min distilled water 10 min 80% alcohol 10 min 90% alcohol 10 min 95% alcohol 10 min absolute alcohol 20 min absolute alcohol 10 min pro-par (xylene substitute) 10 min pro-par 20 min pro-par 10 min paraffin 10 min paraffin 10 min paraffin 10 min paraffin That comes out to be about 3 hours of time once you factor in the pump in/pump out time, and we have a VIP 5. Nathan Jentsch BS HT(ASCP) From Melissa.Kuhnla <@t> chsli.org Fri Apr 22 04:24:29 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri Apr 22 04:24:34 2011 Subject: [Histonet] How to keep paraffin sections from sticking toblade/blade holder. In-Reply-To: <631746.44415.qm@web65716.mail.ac4.yahoo.com> References: <631746.44415.qm@web65716.mail.ac4.yahoo.com> Message-ID: Check the retraction on the back of the mictrotome. Switch to the other setting -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, April 21, 2011 3:26 PM To: Histonet list serv.; BretClough Subject: Re: [Histonet] How to keep paraffin sections from sticking toblade/blade holder. 1- change cutting angle 2- keep the blade holder clean (without paraffin) 3- make sure the blade is not blunt Ren? J. ? ? --- On Thu, 4/21/11, Clough, Bret wrote: From: Clough, Bret Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. To: "Histonet list serv." Date: Thursday, April 21, 2011, 3:07 PM While sectioning paraffin embedded tissue on a rotary? microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise? for me. Thanks in advance for your suggestions . Bret _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From lpwenk <@t> sbcglobal.net Fri Apr 22 04:56:46 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Apr 22 04:57:32 2011 Subject: [Histonet] Input needed In-Reply-To: References: Message-ID: You've picked a great profession for lots of vacancies. Of course, at any given moment, in any given location, there might not be an opening, but if you are willing/able to move, there are lots of openings. And many places are willing to pay moving expenses, or sign on bonuses. Just ask, if they don't advertise that they might be willing to pay some extra. ASCP "LabMedicine" just published their wage and vacancy survey results in March and April 2011 issues. (Thank you to everyone who is an ASCP member, whose dues help pay for this!) Vacancy: http://labmed.ascpjournals.org/content/42/4/199.full.pdf+html Wages: http://labmed.ascpjournals.org/content/42/3/141.full.pdf+html (Please note, the wages listed are the median wages of everyone. And the average number of years that people were working that were reporting there wages was usually 10-15 years. So the wages reported are NOT the beginning salary.) Here's my summary of the vacancy rates: Histology Tech Vacancy Rate = 8.5% (second highest in survey) Histology Supervisor Vacancy Rate = 17.8% (3rd highest in survey) Histotech Overtime/Double shifts = 20.9% (second highest in survey) Time to fill histotech position: - 26.7% took less than 3 months - 34.2% took 3-4 months - 25.3% took 6-12 months - 13.7% took more than a year (highest in the survey) (That's why asking for hiring bonus or moving expenses works sometimes. Almost 40% take more than half a year to find someone!) Time to fill histology supervisor positions: - 26.0% took less than 3 months - 26.7% took 3-6 months - 23.3% took 6-12 months - 24.0% took more than a year (highest in the survey) Percent histotechs who will retire in next 5 years - 15.3% (2nd highest in survey) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Mary" Sent: Thursday, April 21, 2011 6:06 PM To: Subject: [Histonet] Input needed > What?s the chance of getting a job in histology as a new grad at age 57. > Will gender be an issue? Thanks for the input. Jason > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruebenjcarter <@t> gmail.com Fri Apr 22 06:09:08 2011 From: ruebenjcarter <@t> gmail.com (R C) Date: Fri Apr 22 06:09:11 2011 Subject: [Histonet] Histologist looking for work in San Diego Message-ID: I have 8 years clinical, 3 years pharmaceutical research. Well rounded (embedding, microtomy/cryotomy, special stains, IHC, ISH, Digital Pathology/Image Analysis, double-staining. Looking for position close to San Diego. Please contact me for CV or any leads. Thanks. From benjamin.ahlijah <@t> bioreliance.com Fri Apr 22 08:03:10 2011 From: benjamin.ahlijah <@t> bioreliance.com (Ahlijah, Benjamin) Date: Fri Apr 22 08:03:25 2011 Subject: [Histonet] (no subject) In-Reply-To: <193223.55791.qm@web84301.mail.re1.yahoo.com> References: <193223.55791.qm@web84301.mail.re1.yahoo.com> Message-ID: We also use Neutralex. Regards, Ben. Benjamin Ahlijah, BS Pathology Lab Supervisor BioReliance Corporation 14920 Broschart Road Rockville, MD 20850 Office: 301-610-2602 Fax:301-610 2199 benjamin.ahlijah@BioReliance.com Please consider the environment before printing this e-mail BioReliance Corporation, "Celebrating 60 years of contract bioservices" This message is intended for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or if you have reason to believe you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you received this communication in error, please notify us immediately. Thank you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON Sent: Thursday, April 21, 2011 12:37 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi from Southern Cali, We neutralize our formalin with a product called Neutralex. It comes in powder form and after?30 mins we can dump it down the drain. We also keep records?for?QC purposes.?Product is?purchased from a company called American MasterTech. Hope this gives you a starting place. ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Apr 22 08:10:59 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Apr 22 08:11:04 2011 Subject: [Histonet] Input needed In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974BC3@is-e2k3.grhs.net> Age and gender should not play a role in your chances of getting a job. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Sent: Thursday, April 21, 2011 5:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Input needed What's the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Fri Apr 22 08:14:00 2011 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Apr 22 08:14:07 2011 Subject: [Histonet] RE: Neutralizing Formalin In-Reply-To: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA65@MAIL-AG-CLUSTER.lha.org> References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA65@MAIL-AG-CLUSTER.lha.org> Message-ID: We bought materials for neutralizing formalin and started to use them. Two years later, the local EPA authorities told us to stop because we "lacked the competence to neutralize hazardous materials." Our neutralizing solution became a "hazardous material." We now pay a waste hauler to take our formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Thursday, April 21, 2011 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neutralizing Formalin Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!! Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Apr 22 08:22:21 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Apr 22 08:22:25 2011 Subject: [Histonet] A survey of IHC lab staffing Message-ID: Good morning, I am sending out one of those tedious surveys for people to answer if they feel so compelled and have the interest/time. Ours is an automated lab with Ventana instruments, 2 XTs and 1 BMK but I encourage anyone who wishes, to answer. We are currently moving from a 2 permanent FTE IHC lab to a 1 permanent FTE plus 1 FTE from a pool of 6 rotating surgical pathology techs. As you may have guessed, I am the 1 permanent FTE and I'm having a difficult time envisioning how this rotation of techs is going to work. These are my questions off the top of my head for now: * What is your monthly/yearly IHC workload? * How many techs rotate through IHC? * Are there any permanent techs in IHC that don't rotate? * Do all the techs, permanent or rotating, perform all the duties of the IHC lab? * What is your schedule of rotation? Daily? Weekly? Monthly? Other? * What are the hours of your IHC lab? The same as for the histology lab? * How many people are working in the IHC lab at any given time? If it varies, how many hours are one, two, more people staffing the IHC lab during the day? * Do you have more than one shift working in IHC? * Do you feel that all the people working in IHC have about the same level of expertise? If not, how is that dealt with? * Who keeps track of things like antibody, detection, ancillary reagent and supply inventories? * Does this same person do the ordering or do all techs order? * Who does monthly/quarterly cleaning and decontamination of your instruments? * Who is responsible for maintenance of temperatures, pH meter(s), freezer defrosting, making up of bulk solutions i.e. reaction buffer, EZ-Prep, etc. * Do all people, some, or one person deal with QC of new lots of antibodies, detection, etc? * Who is responsible for trouble shooting instrument/staining failures or problems? * Who is responsible for finding and maintaining control tissue inventory? * Who is responsible for workload tallies/recording? * Who is responsible for bringing on new antibodies? * Who is the main contact person with Ventana or other instrument/antibody vendor? * Who does send outs to reference labs for antibodies you don't carry? * Are your requests mostly by panels of antibodies or not? * Are certain antibodies/panels always requested on certain tissue types/diagnoses? * Are some/all/none of your IHC requests cut at the time of the H&E sectioning? * What is your TAT policy for IHC requests, i.e. deadline for same day TAT, from in when to out when? * If you are a VMS automated lab, how many and which VMS instruments do you have? * Do you use all predilute, all concentrate, or some of each kind of antibody? Thank you for your time and input. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From LSebree <@t> uwhealth.org Fri Apr 22 08:34:47 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Apr 22 08:34:52 2011 Subject: [Histonet] A survey of IHC lab staffing Message-ID: Good morning, I am sending out one of those tedious surveys regarding staffing of IHC labs. Ours is an automated lab with Ventana instruments; 2 XTs and 1 BMK but I encourage anyone with the interest/time to respond. We are currently moving from a 2 permanent FTE IHC lab to a 1 permanent FTE plus 1 FTE from a pool of 6 rotating surgical pathology techs. As you may have guessed, I am the 1 permanent FTE and I'm having a difficult time envisioning how this rotation of techs is going to work. These are my questions for now: * What is your monthly/yearly IHC workload? * How many techs rotate through IHC? * Are there any permanent techs in IHC that don't rotate? * Do all the techs, permanent or rotating, perform all the duties of the IHC lab? * What is your schedule of rotation? Daily? Weekly? Monthly? Other? * What are the hours of your IHC lab? The same as for the histology lab? * How many people are working in the IHC lab at any given time? If it varies, how many hours are one, two, more people staffing the IHC lab during the day? * Does your IHC lab operate more than one shift? * Do you feel that all the people working in IHC have about the same level of expertise? If not, how is that dealt with? * Who keeps track of things like antibody, detection, ancillary reagent and supply inventories? * Does this same person do the ordering or do all techs order? * Who does monthly/quarterly cleaning and decontamination of your instruments? * Who is responsible for things like maintenance of temperatures, pH meter(s), freezer defrosting, making up of bulk solutions and antibodies, etc.? * Do all people, some, or one person deal with QC of new lots of antibodies, detection, etc? * Who is responsible for trouble shooting instrument/staining failures or problems? * Who is responsible for finding and maintaining control tissue inventory? * Who is responsible for workload tallies/recording? * Who is responsible for bringing on new antibodies? * Do you use predilute, concentrate or some of each type of antibodies? * Who is the main contact person with Ventana or other antibody/instrument vendor? * Who does send outs to reference labs for antibodies you don't carry? * Are your requests mostly by panels of antibodies or not? * Are certain antibodies/panels always requested on certain tissue types/diagnoses? * Are some/all/none of your IHC requests cut at the time of the H&E sectioning? * What is your TAT policy for IHC requests, i.e. deadline for same day TAT, from in when to out when? * For VMS users, how many and which VMS instruments do you have? * Any opinions on which model of staffing has worked best in your situation? I will compile any results received and send it out on Histonet. Thanks for everyone's time and input, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From LSebree <@t> uwhealth.org Fri Apr 22 08:39:31 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Apr 22 08:39:34 2011 Subject: [Histonet] Sorry about duplicate IHC Lab staffing survey...please respond to only one Message-ID: Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From abuchiane <@t> bmhvt.org Fri Apr 22 08:42:59 2011 From: abuchiane <@t> bmhvt.org (Anita Buchiane) Date: Fri Apr 22 08:43:04 2011 Subject: [Histonet] RE: Neutralizing Formalin In-Reply-To: References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA65@MAIL-AG-CLUSTER.lha.org> Message-ID: <602863D272B56749A70CBA315D7DC70205CC3482@bmhexch.bmhvt.org> We recycle most of our formalin using the Creative Waste system but if we have an especially dirty batch (usually from the monthly dumping of specimens) we neutralize it with "Hyde-Away" (Fisher cat. # 04-355-78) we pay $269 for a case of 4 gallons (uses 8oz.for each gallon of formalin). My lab is in Vermont and as far as I know we comply with the state regs. Hope this helps. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Friday, April 22, 2011 9:14 AM To: Evans, Andria B Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Neutralizing Formalin We bought materials for neutralizing formalin and started to use them. Two years later, the local EPA authorities told us to stop because we "lacked the competence to neutralize hazardous materials." Our neutralizing solution became a "hazardous material." We now pay a waste hauler to take our formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Thursday, April 21, 2011 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neutralizing Formalin Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!! Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. 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The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ From LINDA.MARGRAF <@t> childrens.com Fri Apr 22 08:56:25 2011 From: LINDA.MARGRAF <@t> childrens.com (Linda Margraf) Date: Fri Apr 22 08:56:29 2011 Subject: [Histonet] Unsubscribe In-Reply-To: <91F17A8EA5A1AFDA3FBCAB34@CDYwxp1931.ad.med.buffalo.edu> References: <383929.85727.qm@web125706.mail.ne1.yahoo.com> <81BCDB6F-2770-4C4B-833C-A55143E09806@phylogenyinc.com> <91F17A8EA5A1AFDA3FBCAB34@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <683621D7852C2F488898D0AC7F164A9879541467@CMCPBEXMAIL02.Childrens.med> Hi, I am the Histonet administrator and I just tried to remove the people requesting to leave the Histonet list but both addresses were not on the list so I guess they were successful in removing themselves. Anyone wishing to remove their address from the list or change something about their subscription (ie. update the address, switch to the daily digest version, put on a temporary vacation hold on the mail etc), please go to the website http://lists.utsouthwestern.edu/mailman/listinfo/histonet and change your subscription using the instructions at the bottom of the page. Please remember, if your email address changes at all from when you first subscribed, you must update it on the list, in order to be able to post messages. If you cannot for some reason unsubscribe or have other problems, please let me know. Thanks, Linda M Histonet administrator -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 21, 2011 9:35 AM To: Emily Sours; Mollie Hannon; spanish_fly1981@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unsubscribe **Listserv master: Some people are reporting problems with managing their subscription as they have submitted their unsubscription multiple times in the prescribed manner and are still getting Histonet emails. This was reported to me by one such (failed) unsubscriber.** --On Thursday, April 21, 2011 10:25 AM -0400 Emily Sours wrote: > Jupiter's thunder!!!! > two people in a row? really?!!?!? > LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT > YOU SUBSCRIBED ON. > > Both of you are officially banned from the internet. Forever. Don't use > it again. > And don't EVER join a mailing list. EVER. > > Emily, who would love to unsubscribe you so I didn't have to get your > ridiculous emails about how you can't do it your damn self. > > A great book should leave you with many experiences, and slightly > exhausted. You should live several lives while reading it. > -William Styron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6118 Fx: (716) 829-2665 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From cjbulmer <@t> sbcglobal.net Fri Apr 22 09:12:48 2011 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Fri Apr 22 09:12:50 2011 Subject: [Histonet] Positive controls Message-ID: <988711.32712.qm@web82302.mail.mud.yahoo.com> Hello Histoland, ? Does anyone have a good source for purchasing HSV I & II ( Cocktail) positive controls? ? Thanks, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX From Ashley.Troutman <@t> Vanderbilt.Edu Fri Apr 22 09:46:20 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Apr 22 09:46:25 2011 Subject: [Histonet] Input needed Message-ID: <7B310892042DA74CB3590053F424CFE613D63CD2B4@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi Jason, It is against Federal law to discriminate based on age or gender. And one should not have too much of an issue finding a job, many places are hiring and looking for well-trained techs. Good luck! Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 (Office) 615-875-3311 (Lab) 615-343-9134 Message: 16 Date: Thu, 21 Apr 2011 18:06:19 -0400 From: "Mary" Subject: [Histonet] Input needed To: Message-ID: Content-Type: text/plain; charset="utf-8" What???s the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason From Sandra.Harrison3 <@t> va.gov Fri Apr 22 09:53:00 2011 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Apr 22 09:53:06 2011 Subject: [Histonet] Input needed In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974BC3@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974BC3@is-e2k3.grhs.net> Message-ID: Out of 6 recent hires, I hired two 50+ employees. One had been employed in another department for over 28 years. One was a fairly recent grad. of a Histology program. They were both good employees, though neither had much routine Histology experience. That being said, neither one of them are still here: one chose to retire, after 3 years in our department. The other one decided to return to the previous place of employment within 6 months, stating that the fast pace of our lab was a deciding factor. Bottom line - neither age or gender played a role in their getting hired. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, April 22, 2011 8:11 AM To: Mary; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Input needed Age and gender should not play a role in your chances of getting a job. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Sent: Thursday, April 21, 2011 5:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Input needed What's the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Apr 22 10:24:04 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Apr 22 10:24:10 2011 Subject: [Histonet] RE: Input needed Message-ID: <06253B36AD9646178873C090811C2C76@ownerf1abaad51> Hi Mary and Jason, I am a recruiter who works exclusively in permanent placement of histology professionals nationwide. Here in Orlando Keiser University has a great Histology program and I work with the new grads on job placement. What I have found is that the biggest challenge for a new grad in this economy is the lack of entry level positions. Most of the employers want someone with a minimum of 2 years of experience. I blame it on the economy because 3 years ago I placed as many new grads as experienced techs and nowadays it is very difficult not only for me to place a new grad but for the new grads to find positions themselves. Most labs seem to be shortstaffed so they can't take the time to supervise a new grad. I suggest that you contact the career placement center of the school that has the histology program that you are thinking about attending ask them what their placement rate is for histotechs. Be sure and find out not only how many of their techs get jobs but how many get jobs in the local area and how many needed to relocate to find employment. If there is not a career placement center I suggest that you talk with the program director or one of the instructors. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From TJJ <@t> stowers.org Fri Apr 22 11:50:43 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Apr 22 11:50:50 2011 Subject: [Histonet] Re: endogenous peroxidases Message-ID: Hi Emily, As for when is better to block endogenous peroxidases, I have no direct experience but I have heard from some that it should be done post-AR because the retrieval can reactivate the endogenous peroxidase. So that's when we do it. Some folks worry about what effect that might have on their primary antibody binding with their target and will do their block after application of the primary antibody, prior to the secondary (or whichever step has the HRP label). I try to keep it simple and just do my H2O2 block prior to the protein blocking step. Also, we never use methanol as a solvent for diluting the H2O2. It is contraindicated in some CD marker staining, and it's cheaper and just as good to use aqueous 3% H2O2. Some make it up in buffer, and you can do that as well if you wish. For cryosection peroxidase quenching, we use 0.3% H2O2 for 30 minutes. Otherwise it's a 10 minute step right after the AR in our routine paraffin section IHCs. Hope this helps! Teri Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From Ashley.Troutman <@t> Vanderbilt.Edu Fri Apr 22 12:22:18 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Apr 22 12:22:23 2011 Subject: [Histonet] Positive controls Message-ID: <7B310892042DA74CB3590053F424CFE613D63CD2B8@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi Cindy, We get ours from American Master Tech: HSV1 Item # CSH0425P (Box of 25) HSV2 Item # CSH0525P (Box of 25) I use Cell Marque's antibodies and they cross-react with each other, so you should be able to get by with one or the other. (I would test this in your lab first, though.) Good luck. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 (Office) 615-875-3311 (Lab) 615-343-9134 Message: 13 Date: Fri, 22 Apr 2011 07:12:48 -0700 (PDT) From: Cindy Bulmer Subject: [Histonet] Positive controls To: Histonet Message-ID: <988711.32712.qm@web82302.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histoland, ? Does anyone have a good source for purchasing HSV I & II ( Cocktail) positive controls? ? Thanks, Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX From carl.hobbs <@t> kcl.ac.uk Fri Apr 22 12:27:35 2011 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Apr 22 12:28:48 2011 Subject: [Histonet] endogenous peroxidases Message-ID: <11D9615B89C10747B1C985966A63D7CA367AA53927@KCL-MAIL04.kclad.ds.kcl.ac.uk> Gimme 5! Block before or after Ag retrieval. If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2 in Dist.water. Block for 15mins. Methanol is not neccessary...imho. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus London SE1 1UL Tel: 020 78486813 Fax: 020 78486816 From akemiat3377 <@t> yahoo.com Fri Apr 22 12:42:01 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Apr 22 12:42:29 2011 Subject: [Histonet] endogenous peroxidases In-Reply-To: <11D9615B89C10747B1C985966A63D7CA367AA53927@KCL-MAIL04.kclad.ds.kcl.ac.uk> References: <11D9615B89C10747B1C985966A63D7CA367AA53927@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: <632B5695-55E5-48F4-ADE5-A2EA505B623E@yahoo.com> Make sure you use a fairly fresh bottle of H2O2. (no older than 6 months). The stock bottle degrades after it is opened. I have seen people using an older bottle of H2O2 and the results are sub-optimal. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote: > > Gimme 5! > > Block before or after Ag retrieval. > If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2 > in Dist.water. > Block for 15mins. > Methanol is not neccessary...imho. > Carl > > > > Carl Hobbs > Histology Manager > Wolfson CARD > School of Biomedical Sciences > Kings College London > Guys Campus > London > SE1 1UL > Tel: 020 78486813 > Fax: 020 78486816 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Fri Apr 22 12:50:49 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Apr 22 12:50:53 2011 Subject: [Histonet] endogenous peroxidases In-Reply-To: <632B5695-55E5-48F4-ADE5-A2EA505B623E@yahoo.com> References: <11D9615B89C10747B1C985966A63D7CA367AA53927@KCL-MAIL04.kclad.ds.kcl.ac.uk> <632B5695-55E5-48F4-ADE5-A2EA505B623E@yahoo.com> Message-ID: So I have been seeing from this post that people block prior to HIER? I have always done it as the first step after for 10 minutes (I use the DAKO enzyme blocker). Is this wrong? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, April 22, 2011 12:42 PM To: Hobbs, Carl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] endogenous peroxidases Make sure you use a fairly fresh bottle of H2O2. (no older than 6 months). The stock bottle degrades after it is opened. I have seen people using an older bottle of H2O2 and the results are sub-optimal. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote: > > Gimme 5! > > Block before or after Ag retrieval. > If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2 > in Dist.water. > Block for 15mins. > Methanol is not neccessary...imho. > Carl > > > > Carl Hobbs > Histology Manager > Wolfson CARD > School of Biomedical Sciences > Kings College London > Guys Campus > London > SE1 1UL > Tel: 020 78486813 > Fax: 020 78486816 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.byrnes <@t> accelpath.com Fri Apr 22 12:55:28 2011 From: a.byrnes <@t> accelpath.com (Andrew Byrnes) Date: Fri Apr 22 12:55:34 2011 Subject: [Histonet] Histo lab consultants Message-ID: Dear Histoneters, We are looking for histotechnologists or lab consultants to talk to who are currently working in labs that may have a need for anatomic pathology services. If so, we can make it worth your while. Please give me a call or send me an email: a.byrnes@accelpath.com 732-312-8008 www.accelpath.com Thanks! Andrew From kkmarshall <@t> anthc.org Fri Apr 22 12:58:41 2011 From: kkmarshall <@t> anthc.org (Marshall, Kimberly K) Date: Fri Apr 22 12:59:16 2011 Subject: [Histonet] Howdy all Message-ID: Happy Friday Histo peeps Would anyone know of any openings for Histo techs in the Southern Utah area? I have a tech working with me that is looking to relocate from Alaska to Utah to be closer to family. Any info would be great. Thanks Kimberly From ruppert.amysue <@t> marshfieldclinic.org Fri Apr 22 13:14:35 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Fri Apr 22 13:14:39 2011 Subject: [Histonet] hematoxlin hue on slides&In-Reply-To= Message-ID: <201104221814.p3MIEbF9024320@mailhost2.mfldclin.edu> Are you using any adhesive in the tissue water bath? We have had issues of stain being picked up on the glass slide from certain brands. The amount of this hue can vary from lot to lot. amysue ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Fri Apr 22 14:27:19 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 22 14:27:22 2011 Subject: [Histonet] endogenous peroxidases In-Reply-To: Message-ID: <596403.93621.qm@web65715.mail.ac4.yahoo.com> My sequence is: dewax (with dishwasher soap) ? wash ??HIER ? wash ? block endogenous peroxidase ? go tthe IHC protocol. Ren? J. --- On Fri, 4/22/11, sgoebel@mirnarx.com wrote: From: sgoebel@mirnarx.com Subject: RE: [Histonet] endogenous peroxidases To: akemiat3377@yahoo.com, carl.hobbs@kcl.ac.uk Cc: histonet@lists.utsouthwestern.edu Date: Friday, April 22, 2011, 1:50 PM So I have been seeing from this post that people block prior to HIER?? I have always done it as the first step after for 10 minutes (I use the DAKO enzyme blocker).? Is this wrong? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, April 22, 2011 12:42 PM To: Hobbs, Carl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] endogenous peroxidases Make sure you use a fairly fresh bottle of H2O2. (no older than 6? months).? The stock bottle degrades after it is opened.? I have seen? people using an older bottle of? H2O2 and the results are sub-optimal. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote: > > Gimme 5! > > Block before or after Ag retrieval. > If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2? > in Dist.water. >? Block for 15mins. > Methanol is not neccessary...imho. > Carl > > > > Carl Hobbs > Histology Manager > Wolfson CARD > School of Biomedical Sciences > Kings College London > Guys Campus > London > SE1 1UL > Tel: 020 78486813 > Fax: 020 78486816 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SDeloney <@t> unch.unc.edu Fri Apr 22 14:46:24 2011 From: SDeloney <@t> unch.unc.edu (Deloney, Sheila) Date: Fri Apr 22 14:46:49 2011 Subject: [Histonet] HTL positions open at UNC Hospitals Message-ID: <6848CED124257946A388AB84A96D4C908D90EA@MSX6.unch.unc.edu> Hi Histonetters!! UNC Hospitals is currently searching for: A Histotechnologists proficient in all aspects of routine Histology, including tissue processing, embedding, sectioning, and maintenance of equipment. In addition, advanced skills in Muscle processing and Histochemistry, Immunohistochemistry, special stains and frozen tissue sectioning are preferred. Requirements include a minimum of one (1) year of histology experience, with HTL (ASCP) certification or eligibility as defined by the ASCP. Uncertified candidates must obtain certification within 18 months of hire. Various shifts available. Scheduled work hours may vary for training purposes and departmental coverage needs. A Histotechnologist for Electron Microscopy and Muscle Pathology services. The HTL-EM/Muscles must be technically proficient in all aspects of clinical electron microscopy. Skills required include tissue collection, freezing, fixation, processing, plastic embedding, block trimming, ultra-microtomy, staining, EM scanning and micrography. Experience with muscle pathology or routine histology procedures is a plus. This individual must be able to work independently and function with minimal supervision. Work hours are Monday-Friday, with varying start times ranging from 6:00 AM to 8:00 AM depending on EM scope availability and case volume, with occasional rotating Saturdays. Scheduled work hours may vary for training purposes and departmental coverage needs. To apply or learn more about our career opportunities, please visit our website at www.unchealthcare.org/jobs. Sheila L Deloney Histology Laboratory Supervisor UNC Hospitals/ McLendon Clinical Labs 101 Manning Drive W&C Hospitals RM CH30231 Chapel Hill, NC 27514 Ph: 919-843-1082 / 966-6288 fax Pager: 919-347-1677 Email: sdeloney@unch.unc.edu From Wanda.Smith <@t> HCAhealthcare.com Fri Apr 22 15:04:18 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Apr 22 15:04:26 2011 Subject: [Histonet] A PRN Histotech Needed in Charleston, SC Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A535C5A9@NADCWPMSGCMS03.hca.corpad.net> I have an open PRN HT position open at Trident Medical Center in Charleston, SC. The website to apply is TridentHealthSystem.com under careers/For Professionals/search jobs/apply online. The job # is 00062-8978. Ideal hours would be flexible if possible (mornings or afternoons), but priority is early morning to help with microtomy. Great crew to work with and good equipment (2 Shandon's and 2 Microm's) Special rate for PRN HT. Description states BS degree and NACCLS program, but it should read "or" NACCLS program. Call me below with question or apply on-line. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From dlaudier <@t> gmail.com Sat Apr 23 05:22:35 2011 From: dlaudier <@t> gmail.com (Damien Laudier) Date: Sat Apr 23 05:22:38 2011 Subject: [Histonet] Re: Deplasticizing before staining Message-ID: From dlaudier <@t> gmail.com Sat Apr 23 05:29:19 2011 From: dlaudier <@t> gmail.com (Damien Laudier) Date: Sat Apr 23 05:29:22 2011 Subject: [Histonet] Re:Deplasticizing before staining Message-ID: Hi Mahesh, The type of thick plastic sections you're inquiring about usually require "etching" with a dilute alcohol or acid before staining to facilitate stain penetration. However some stains will still penetrate without this pre-treatment if heat is used during staining. Feel free to contact me for more information on technique if you'd like. -Damien L. From hrumbut <@t> yahoo.com Sat Apr 23 13:31:59 2011 From: hrumbut <@t> yahoo.com (Heather R) Date: Sat Apr 23 13:32:03 2011 Subject: [Histonet] Processors and power outages Message-ID: <80495.29263.qm@web130224.mail.mud.yahoo.com> Wondering what the labs who are not in hospital setting, are doing with thier processors?for power outages. Starting a derm lab? and want to address this issue. Thanks H. From gloria.cole <@t> usa.net Sun Apr 24 15:30:57 2011 From: gloria.cole <@t> usa.net (Gloria Cole) Date: Sun Apr 24 15:31:09 2011 Subject: [Histonet] Histotech position in Irving, Texas Message-ID: <55D857E3-9B4D-4278-9568-E1836A8CD302@usa.net> Hello, Just wanted to let everyone know I have one and may have another Histotech/ Grossing tech position open in our GI lab in Irving, Texas. The hours on one position are negotiable the other will be day shift. If you are interested or know anyone interested please contact me for more details. Thanks, Gloria Cole BS HT (ASCP) NuePath Supervisor 972-489-2071 gcole@nueterrapathology.com From amber.mckenzie <@t> gastrodocs.net Mon Apr 25 08:13:07 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Apr 25 08:13:09 2011 Subject: [Histonet] Processors and power outages In-Reply-To: <80495.29263.qm@web130224.mail.mud.yahoo.com> References: <80495.29263.qm@web130224.mail.mud.yahoo.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3704107AFF@giamail2.Gia.com> Back-up battery -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather R Sent: Saturday, April 23, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processors and power outages Wondering what the labs who are not in hospital setting, are doing with thier processors?for power outages. Starting a derm lab? and want to address this issue. Thanks H. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Mon Apr 25 09:02:10 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Apr 25 09:02:23 2011 Subject: [Histonet] FDA approved IVD antibody Message-ID: <24A4826E8EF0964D86BC5317306F58A55DF526D86C@mmc-mail.ad.mhsil.com> Question - I am trying to figure why we should switch to an FDA approved IVD antibody for Cd117 from the current IVD antibody we use that is not FDA approved. Most of the IHC antibodies we use are not FDA approved. I am fully aware of the extra things we have to do if an antibody is a RUO or ASR antibody but am a little questioned about the true advantage of the FDA approval. The cost of course for an FDA approved antibody is extremely high in comparison to other IVD antibodies that are not FDA approved. (as much as five times as much). In this case I am trying to figure out what is so special about Cd117. If someone could enlighten me I would be grateful. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From brian <@t> prometheushealthcare.com Mon Apr 25 09:50:39 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Mon Apr 25 09:50:52 2011 Subject: [Histonet] Happy National Medical Laboratory Professionals Week Message-ID: <00aa01cc0358$281e1fd0$785a5f70$@com> National Medical Laboratory Professionals Week is an annual celebration of the medical laboratory professionals and pathologists who play a vital role in every aspect of health care. This year we will be celebrating from April 24th through April 30th. NMLPW is a chance for medical laboratory personnel to celebrate their professionalism and be recognized for their efforts. Often, they use this time to inform and educate medical colleagues and the public about the medical laboratory. It's estimated that up to 70 percent of medical decisions are made based on the test results generated by these highly-skilled professionals. Thanks to the 265,000 medical laboratory professionals and 15,000 board-certified Pathologists who play a vital role in every aspect of health care!!! Happy Lab Professionals Week! Prometheus Healthcare ( www.prometheushealthcare.com) is committed to connecting dedicated lab professionals with top medical organizations nationwide. Prometheus Healthcare Office 301-693-9057 Cell 301-605-5450 Fax 301-368-2478 info@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From dmikita <@t> wmcnet.org Mon Apr 25 12:25:53 2011 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Mon Apr 25 12:25:55 2011 Subject: [Histonet] Processors and power outages Message-ID: <4DB55A3D.1875.003A.0@wmcnet.org> What type of battery back-up are you using. The type that is purchased from a local office supply store is to low of amps to supply the processor with power. I don't think that the power supply would be large enough to supply power for a couple of hours, or is it? Thanks, Daryl Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 Phone: 307-577-2198 Fax: 307-577-2731 From trathborne <@t> somerset-healthcare.com Mon Apr 25 12:37:25 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Apr 25 12:38:20 2011 Subject: [Histonet] Processors and power outages In-Reply-To: <4DB55A3D.1875.003A.0@wmcnet.org> Message-ID: Does the processor manufacturer have any suggestions? Is there an alarm that can be hooked up to a remote site (someone's home or phone/computer)? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Daryl Mikita Sent: Monday, April 25, 2011 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processors and power outages What type of battery back-up are you using. The type that is purchased from a local office supply store is to low of amps to supply the processor with power. I don't think that the power supply would be large enough to supply power for a couple of hours, or is it? Thanks, Daryl Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 Phone: 307-577-2198 Fax: 307-577-2731 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From nelsonrnch <@t> verizon.net Mon Apr 25 12:41:25 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Mon Apr 25 12:41:28 2011 Subject: [Histonet] (no subject) Message-ID: <648242.71204.qm@web84303.mail.re1.yahoo.com> First of all make sure your designated outlet?has its own power surge?within?it.?Talk with your building electrician. They should?be able to accommodate you. If you still feel you need extra?protection you maybe able to?just install a power?surge protector. ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net From historsd <@t> aol.com Mon Apr 25 14:37:45 2011 From: historsd <@t> aol.com (historsd@aol.com) Date: Mon Apr 25 14:37:59 2011 Subject: [Histonet] automatic IHC Message-ID: <8CDD19762A950E7-19D4-209B2@webmail-d052.sysops.aol.com> Does anyone have any experience with the WAVE RPD system by Celerus diagnostics? Specifically for use in a Mohs lab? Any advice would be useful. Thanks in advance. I can be contacted at historsd@aol.com. From robin_dean <@t> compbio.com Mon Apr 25 17:09:49 2011 From: robin_dean <@t> compbio.com (Robin Dean) Date: Mon Apr 25 17:13:02 2011 Subject: [Histonet] silver stain on slide-mounted FFPE brain tissues Message-ID: <002b01cc0395$7ef46ca0$7cdd45e0$@com> Hi Histonetters, We want to do a degeneration-selective silver stain on slide-mounted formalin-fixed paraffin-embedded monkey brain sections. It looks like most of the kits/protocols are for free floating tissue sections or chunks of tissue that are stained and then sectioned. Has anyone used this stain or a similar stain on slide -mounted brain sections? (something more like an IHC protocol). We need to do multiple different stains on sections from the same brain, so we don't want to silver stain a whole chunk of brain tissue, just some individual sections. I'd appreciate any suggestions or help anyone might have to offer. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com From Vickroy.Jim <@t> mhsil.com Tue Apr 26 07:03:03 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Apr 26 07:03:10 2011 Subject: [Histonet] grossing stations Message-ID: <24A4826E8EF0964D86BC5317306F58A55DF526DAEF@mmc-mail.ad.mhsil.com> We are looking into new grossing stations. Can anybody give me their opinions on which to choose, experiences, and what to avoid? 1. Mopec's Grossing Stations 2. ThermoFisher Gross Lab Senior 3. Accu-Edge Workstations by Sakura James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From jsu5590m <@t> jsu.edu Tue Apr 26 08:27:15 2011 From: jsu5590m <@t> jsu.edu (Lindsey Minton) Date: Tue Apr 26 08:27:54 2011 Subject: [Histonet] HM 335 e manual In-Reply-To: <957814189.10472511303824313652.JavaMail.root@mbs1.jsu.edu> Message-ID: <2027327270.10473221303824435008.JavaMail.root@mbs1.jsu.edu> I'm hoping someone can help me out. Our university has acquired a Microm HM 335 e Microtome, but we have no instruction manual. So far, the internet has yielded no assistance. Does anyone know where I can find/get one, preferably free? Lindsey M. Minton From jsu5590m <@t> jsu.edu Tue Apr 26 09:08:24 2011 From: jsu5590m <@t> jsu.edu (Lindsey Minton) Date: Tue Apr 26 09:08:49 2011 Subject: [Histonet] Thanks! Message-ID: <1297249040.10490181303826904324.JavaMail.root@mbs1.jsu.edu> Thanks Histonet and all of you that responded! I almost immediately got a response from a Thermo Fisher tech (Marganne you rock!) with the PDF. Lindsey M. Minton Jacksonville State University From sfonner <@t> labpath.com Tue Apr 26 09:07:13 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Tue Apr 26 09:10:05 2011 Subject: [Histonet] Rickettsia Antibody Message-ID: <001a01cc041b$3df22810$b9d67830$@com> Hi everyone, One of our pathologists is wanting to know if anyone has an antibody they are using for rickettsia (rocky mountain spotted fever). We used to send this out but our supplier no longer offers it and did not know of anyone else doing it. I would greatly appreciate any information you could give me. Thanks Histoland! Sheila, HT (ASCP) KDL Pathology Knoxville, TN From rceades <@t> gmail.com Tue Apr 26 10:13:16 2011 From: rceades <@t> gmail.com (Eric Eades) Date: Tue Apr 26 10:13:23 2011 Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. In-Reply-To: References: Message-ID: Hi Bret, If you grab the first section as it comes off the blade with a pair of forceps, then your ribbon never needs to touch the blade holder at all. Just don't use your fingers! Good luck, -Eric On Thu, Apr 21, 2011 at 12:07 PM, Clough, Bret wrote: > While sectioning paraffin embedded tissue on a rotary microtome I noticed > the sections sticking to the blade holder and bunching up. What do I need to > do so that my section come off the blade in a ribbon? Please note that this > is my first time in sectioning tissue and this is a learning exercise for > me. > > Thanks in advance for your suggestions . > > Bret > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sgoebel <@t> mirnarx.com Tue Apr 26 10:30:52 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Apr 26 10:30:55 2011 Subject: [Histonet] Positive control Message-ID: Does anyone know what the positive control for MET is? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From ksbrown <@t> lmhosp.org Tue Apr 26 10:42:06 2011 From: ksbrown <@t> lmhosp.org (Brown, Kimberly) Date: Tue Apr 26 10:41:45 2011 Subject: [Histonet] Unscribe Message-ID: Histonet, I like this site, but I am receiving way too much emails for one day. I have requested to be removed from the mailing list several times to only receive more emails. Am I inscribing incorrectly? Please help me dissolve this relationship. Thanks, Kimberly Brown Histology Lab Manager Lawrence and Memorial Hospital Phone # 860-442-0711 ext. 4383 This message (and any included attachments) is from Lawrence & Memorial Corporation, Inc. or one of its affiliates and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. From rjbuesa <@t> yahoo.com Tue Apr 26 10:51:54 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 26 10:51:58 2011 Subject: [Histonet] Unscribe In-Reply-To: Message-ID: <859203.18550.qm@web65711.mail.ac4.yahoo.com> Go to the same website where your "inscribed" yourself, follow the instructions and "unscribe" yourself. You are the ONLY one that can do that. Nobody can (or will) do it for you. Ren? J. --- On Tue, 4/26/11, Brown, Kimberly wrote: From: Brown, Kimberly Subject: [Histonet] Unscribe To: "Histonet@lists.utsouthwestern.edu" Date: Tuesday, April 26, 2011, 11:42 AM Histonet, I like this site, but I am receiving way too much emails for one day.? I have requested to be removed from the mailing list several times to only receive more emails.? Am I inscribing incorrectly?? Please help me dissolve this relationship. Thanks, Kimberly Brown Histology Lab Manager Lawrence and Memorial Hospital Phone #? 860-442-0711 ext. 4383 This message (and any included attachments) is from Lawrence & Memorial Corporation, Inc. or one of its affiliates and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From etambutte <@t> centrescientifique.mc Tue Apr 26 12:06:28 2011 From: etambutte <@t> centrescientifique.mc (Eric Tambutte) Date: Tue Apr 26 12:06:33 2011 Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 30 Message-ID: <1050319054@s15272523.onlinehome-server.info> Merci pour votre message. Je suis actuellement absent du laboratoire. Je repondrai a votre message a mon retour a partir du 3 mai 2011. Thank you for your email. I am currently out of the lab. I will reply to your message after the 3rd of may 2011. From mlm11 <@t> cornell.edu Tue Apr 26 12:14:00 2011 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Tue Apr 26 12:14:06 2011 Subject: [Histonet] unsubscribe Message-ID: <4010584DE26D90419367C0A5F620E963302C095EB4@MBXB.exchange.cornell.edu> From Margaret.Perry <@t> sdstate.edu Tue Apr 26 12:48:49 2011 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Apr 26 12:48:55 2011 Subject: [Histonet] canine teeth Message-ID: Hi I was given blocks today of a canine tooth that had been decaled for 8 days. We processed it on our regular overnight program. It was too hard to cut and popped out of the paraffin. The biopsy trim sheet did not indicate it was a tooth so I put the pieces in ammonium hydroxide for 10 min. It didn't help. Do you have any suggestion on how I can save this tissue? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From kbowden <@t> ucsd.edu Tue Apr 26 13:14:35 2011 From: kbowden <@t> ucsd.edu (kbowden) Date: Tue Apr 26 13:14:39 2011 Subject: [Histonet] canine teeth In-Reply-To: References: Message-ID: <4DB70B8B.5010103@ucsd.edu> I would decal with TBD-2 for about two weeks. TBD-2 is a formic acid decal solution. I would shake the specimen 24 hrs a day. Vacuum and release the specimen several times a day. Change the decal every couple of days. Do not remove the paraffin it helps to protect from over decalcifying. *//* */-- You are what you do - not what you say --/* */Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 3525 John Hopkins Ct. 0863 San Diego, CA 92121-08630 858-822-1251 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: /**/THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER/**/./* On 4/26/11 10:48 AM, Perry, Margaret wrote: > Hi > I was given blocks today of a canine tooth that had been decaled for 8 days. We processed it on our regular overnight program. It was too hard to cut and popped out of the paraffin. The biopsy trim sheet did not indicate it was a tooth so I put the pieces in ammonium hydroxide for 10 min. It didn't help. Do you have any suggestion on how I can save this tissue? > > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services > Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dwenzel01 <@t> gmail.com Tue Apr 26 13:25:26 2011 From: dwenzel01 <@t> gmail.com (Dawn Herron) Date: Tue Apr 26 13:25:30 2011 Subject: [Histonet] Recycling slide brite Message-ID: Hello histonetters. We have made the transition from xylene to using slide brite in our stainer and for coverslipping and have worked out all the kinks except one---recycling it successfully. We've been talking to our recycler manufacturer (B&R) and the manufacturer of slide brite and still haven't had a successful run. Does anyone out there use this product and recycle it? If so could you send me your recycling protocol? Any thoughts would be appreciated. Thanks, Dawn From mchelle252002 <@t> yahoo.com Tue Apr 26 13:45:19 2011 From: mchelle252002 <@t> yahoo.com (Michelle Mathews) Date: Tue Apr 26 13:45:21 2011 Subject: [Histonet] Positive Met control Message-ID: <102655.78207.qm@web161308.mail.bf1.yahoo.com> Sarah, I worked this antibody up using normal colon. Uterine cervix, lung, and gallbladder (all normal) should also stain nicely. Michelle Mathews, HT(ASCP) Research Specialist TPL Does anyone know what the positive control for MET is? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From rjbuesa <@t> yahoo.com Tue Apr 26 14:51:15 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 26 14:51:18 2011 Subject: [Histonet] Recycling slide brite In-Reply-To: Message-ID: <253407.14943.qm@web65715.mail.ac4.yahoo.com> Being a MIXTURE of hydrocarbons, it will crack at different temperatures (separately). You would have to reconstitute it?using the same proportions (usually "proprietary"). Ren? J. --- On Tue, 4/26/11, Dawn Herron wrote: From: Dawn Herron Subject: [Histonet] Recycling slide brite To: Histonet@lists.utsouthwestern.edu Date: Tuesday, April 26, 2011, 2:25 PM Hello histonetters. We have made the transition from xylene to using slide brite in our stainer and for coverslipping and have worked out all the kinks except one---recycling it successfully. We've been talking to our recycler manufacturer (B&R) and the manufacturer of slide brite and still haven't had a successful run. Does anyone out there use this product and recycle it? If so could you send me your recycling protocol? Any thoughts would be appreciated. Thanks, Dawn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cindy38017 <@t> yahoo.com Tue Apr 26 14:54:56 2011 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Tue Apr 26 14:54:59 2011 Subject: [Histonet] tissue artifact Message-ID: <924580.42756.qm@web37104.mail.mud.yahoo.com> I work in a dermpath lab. One day last week we had a few slides that had "vacuoles" or holes next to the nucleus. We had over 600 hundred cases, and it only happened to a few of them. There seems to be nothing in common with them. The biopsies came from different Dr's offices, different companies manufactured the formalin bottles, etc. It only happened one day and since then there have been no issues. Since it does not involve all of the specimens, it doesn't seem to be processing or staining. Could it be from sectioning due to freeze spray? too many sponges?. If anyone has any ideas as to what could cause this, I would greatly appreciate it. From rjbuesa <@t> yahoo.com Tue Apr 26 14:59:59 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 26 15:00:02 2011 Subject: [Histonet] tissue artifact In-Reply-To: <924580.42756.qm@web37104.mail.mud.yahoo.com> Message-ID: <679210.25276.qm@web65715.mail.ac4.yahoo.com> This vacuolation was caused by you. The slides were not properly drained (elimination of ALL the water from the "back" of the section) before they were put on the oven to dry before staining. Under separate cover I am sending you an article on the subject. Ren? J. --- On Tue, 4/26/11, cindy dewar wrote: From: cindy dewar Subject: [Histonet] tissue artifact To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 26, 2011, 3:54 PM I work in a dermpath lab. One day last week we had a few slides that had "vacuoles" or holes next to the nucleus. We had over 600 hundred cases, and it only happened to a few of them. There seems to be nothing in common with them. The biopsies came from different Dr's offices, different companies manufactured the formalin bottles, etc. It only happened one day and since then there have been no issues. Since it does not involve all of the specimens, it doesn't seem to be processing or staining. Could it be from sectioning due to freeze spray? too many sponges?. If anyone has any ideas as to what could cause this, I would greatly appreciate it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlukey <@t> msn.com Tue Apr 26 15:48:09 2011 From: hlukey <@t> msn.com (Hugh Luk) Date: Tue Apr 26 15:48:14 2011 Subject: [Histonet] silver stain on slide-mounted FFPE brain tissues Message-ID: Dr. Dean, Histotechs are familiar with brain degeneration "Silver Stains" on FFPE cut sections(slides). Common silver stains include Bielchowsky's method for neuritic plaques and neurofibrillary tangles (most requested by my pathologists for degenerative brain disorders), Bodian's method for nerve endings and fibers , Holmes method for axons and nerve fibers, or Palmgren's silver impregnation method for neural fibers. There are other histochemical stains, but these are most common on silvers on brain sections. We have been making our own solutions for years (and years and years...). There are some kits for staining available through vendors such as Newcomer Supply http://www.newcomersupply.com/products-standard-special-stains.php, or American Master Tech http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=KTBIE, and if you know the individual reagents you need, you can buy them from almost all vendors. I have not used (nor have an affiliation with) Newcomer Supply and American Master Tech brain kits, but their other products have been great. As for staining whole chunks, I have not seen or done this. It sounds interesting. Hope this helps, Hugh, HTL (ASCP) Pathology core manager Hawaii > From: robin_dean@compbio.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 25 Apr 2011 15:09:49 -0700 > Subject: [Histonet] silver stain on slide-mounted FFPE brain tissues > > Hi Histonetters, > > > > We want to do a degeneration-selective silver stain on slide-mounted > formalin-fixed paraffin-embedded monkey brain sections. It looks like most > of the kits/protocols are for free floating tissue sections or chunks of > tissue that are stained and then sectioned. Has anyone used this stain or a > similar stain on slide -mounted brain sections? (something more like an > IHC protocol). We need to do multiple different stains on sections from the > same brain, so we don't want to silver stain a whole chunk of brain tissue, > just some individual sections. I'd appreciate any suggestions or help anyone > might have to offer. > > > > Thank you, > > > > Robin > > > > Robin R. Dean, Ph.D. > > Senior Scientist & Study Director > > Comparative Biosciences, Inc. > > 786 Lucerne Dr. > > Sunnyvale, CA > > (408) 738-8060 > > robin_dean@compbio.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Apr 26 16:19:33 2011 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Apr 26 16:19:52 2011 Subject: [Histonet] Mohs Key Performane Indicators (Recuts) In-Reply-To: References: Message-ID: I would think that the reason for the recut is relevant to the discussion. We have a multi surgeon Mohs Surgery clinic and I've tracked recut requests by surgeon and the data illustrates what I already knew. We have one surgeon who prefers to go deeper into virtually every block. In this instance it is not a quality indicator but rather an indicator of practice style. It sounds like your environment is a single surgeon practice which should simplify developing your own quality standards. I am not aware that such standards exist but you may consider contacting the Mohs College with your question. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 Learn more about Histotechnology Professionals Day at www.nsh.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martino T. Sent: Thursday, April 21, 2011 5:22 PM To: Histo Net Subject: [Histonet] Mohs Key Performane Indicators (Recuts) Hello, I work for a Mohs surgeon who wants to establish key performance indicators (KPIs) for the mohs techs to evaluate daily performance. Does anyone have any information of surveys or standards for the number of recuts per day or per block as a measure for quality Mohs sectioning? She's working with a standard that 4 or less recuts in a day (average 30 to 50 blocks per day) is quality work. Has anyone come up with standards in this regard for Mohs sectioning? Thanks in advance, Martino Tanner "Life can either be accepted or changed. If it is not accepted, it must be changed. If it cannot be changed, then it must be accepted." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Apr 26 16:24:07 2011 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Apr 26 16:24:12 2011 Subject: [Histonet] S100 primary antibody suitable for frozen sections? In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2AB82@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D76830AD2AB82@EXCHANGE3.huntingtonhospital.com> Message-ID: I would appreciate hearing from anyone who has discovered an S100 primary antibody that is confirmed to work on frozen sections using a conventional IHC stain protocol. This will be utilized in a busy Mohs Surgery laboratory where staining time must be kept as brief as possible. The commercially available antibodies I've seen for this antigen are intended for FFPE tissues and initial attempts to adapt them to frozen sections have been unsuccessful. our surgeons prefer to avoid post fixation prior to staining although I'm willing to re-visit this if it is my only option. Thank you everyone. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 From MLunetta <@t> luhcares.org Tue Apr 26 16:29:51 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Tue Apr 26 16:30:11 2011 Subject: [Histonet] PRN Longmont United Hospital, Colorado Message-ID: <4DB6E4EF020000A80005B820@ns.luhcares.org> Hey all, We are looking for a PRN HT(ASCP) to help us out for holidays and the unexpected suprises. Go to LUHCARES.ORG to get al the info and to apply. thanks Matt Lunetta HT(ASCP) Longmont United Hospital From kayakpa <@t> ptd.net Tue Apr 26 17:22:13 2011 From: kayakpa <@t> ptd.net (Jim Kovach) Date: Tue Apr 26 17:22:16 2011 Subject: [Histonet] HM 335 e manual In-Reply-To: <2027327270.10473221303824435008.JavaMail.root@mbs1.jsu.edu> References: <957814189.10472511303824313652.JavaMail.root@mbs1.jsu.edu> <2027327270.10473221303824435008.JavaMail.root@mbs1.jsu.edu> Message-ID: <003801cc0460$64b3d3a0$2e1b7ae0$@net> Contact Thermo at 800.522.7270.They own Microm. Jim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lindsey Minton Sent: Tuesday, April 26, 2011 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HM 335 e manual I'm hoping someone can help me out. Our university has acquired a Microm HM 335 e Microtome, but we have no instruction manual. So far, the internet has yielded no assistance. Does anyone know where I can find/get one, preferably free? Lindsey M. Minton _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Apr 26 20:21:58 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 26 20:22:12 2011 Subject: [Histonet] Re: processing times for skin specimens In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157188523F9@xmdb02.nch.kids> This is important. Ensure that your fixation time is as long as possible. Short processing will give poor results if fixation is shortened Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Friday, 22 April 2011 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: processing times for skin specimens We do a short run some mornings for shaves and small punches. Keep in mind that we don't work 24/7, so these have been sitting in formalin overnight. 20 min formalin 1 min distilled water 10 min 80% alcohol 10 min 90% alcohol 10 min 95% alcohol 10 min absolute alcohol 20 min absolute alcohol 10 min pro-par (xylene substitute) 10 min pro-par 20 min pro-par 10 min paraffin 10 min paraffin 10 min paraffin 10 min paraffin That comes out to be about 3 hours of time once you factor in the pump in/pump out time, and we have a VIP 5. Nathan Jentsch BS HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Tue Apr 26 20:37:18 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 26 20:37:28 2011 Subject: [Histonet] RE: endogenous peroxidases In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71571885244F@xmdb02.nch.kids> An interesting article by Chun Gao, Ai-Ying Wang, and Ya-Jing Han (Appl Immunohistochem Mol Morphol 2008;16:393-399) concluded that blockage of endogenous peroxidase with H2O2 in immunostaining could be omitted and replaced by microwave oven heating (using 0.01M citric acid buffer (pH 6.0)). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Saturday, 23 April 2011 2:51 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: endogenous peroxidases Hi Emily, As for when is better to block endogenous peroxidases, I have no direct experience but I have heard from some that it should be done post-AR because the retrieval can reactivate the endogenous peroxidase. So that's when we do it. Some folks worry about what effect that might have on their primary antibody binding with their target and will do their block after application of the primary antibody, prior to the secondary (or whichever step has the HRP label). I try to keep it simple and just do my H2O2 block prior to the protein blocking step. Also, we never use methanol as a solvent for diluting the H2O2. It is contraindicated in some CD marker staining, and it's cheaper and just as good to use aqueous 3% H2O2. Some make it up in buffer, and you can do that as well if you wish. For cryosection peroxidase quenching, we use 0.3% H2O2 for 30 minutes. Otherwise it's a 10 minute step right after the AR in our routine paraffin section IHCs. Hope this helps! Teri Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From ccrowder <@t> vetmed.lsu.edu Tue Apr 26 20:48:56 2011 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Tue Apr 26 20:52:46 2011 Subject: [Histonet] Sections sticking Message-ID: Bret - You can also check the back side of your blade. We wipe the front of the blade often, but forget about the back. If paraffin builds up on the blade the sections will stick. Cheryl From ree3 <@t> leicester.ac.uk Wed Apr 27 04:28:49 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Apr 27 04:29:02 2011 Subject: [Histonet] Input needed In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A1017685EBEA1B@EXC-MBX3.cfs.le.ac.uk> Hi Mary, I was made redundant after almost 40 years with the UK's Medical Research Council at the age of 60 in 2008, I applied for several jobs, was offered interviews for all of them, got the first one at the local university, the down side is that I am on a contract, which has been renewed twice so far but now runs out in August this year and I am not so hopeful as money is tight, so go for it Mary, you have many years of experience and skills and much to offer a future employer and new colleagues. Cheers Richard Edwards -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Sent: 21 April 2011 23:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Input needed What?s the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkmarshall <@t> anthc.org Wed Apr 27 07:21:27 2011 From: kkmarshall <@t> anthc.org (Marshall, Kimberly K) Date: Wed Apr 27 07:21:47 2011 Subject: [Histonet] Biopsy Run Message-ID: Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil "nuclear artifact". We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim From tatiana.gonzalez <@t> leo-pharma.com Wed Apr 27 07:38:27 2011 From: tatiana.gonzalez <@t> leo-pharma.com (tatiana.gonzalez@leo-pharma.com) Date: Wed Apr 27 07:38:35 2011 Subject: [Histonet] In vivo staining Message-ID: I am searching for a method to detect how far a dye will penetrate into the skin after applying different barrier disruption methods. My hope was to be able to detect how far into the epidermis the dye penetrates by looking at the samples in a microscope. Do any of you have suggestions on which dye I could use and a protocol that is likely to work for this purpose? The simpler the better! Thanks. Tatiana This e-mail, inclusive of attachments, is intended for the person(s) stated above and may contain confidential information. Unauthorised reading, disclosure, copying, distribution or use of this information may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete your copy. Thank you. From kim.tournear <@t> yahoo.com Wed Apr 27 07:55:37 2011 From: kim.tournear <@t> yahoo.com (Yahoo) Date: Wed Apr 27 07:55:53 2011 Subject: [Histonet] Biopsy Run In-Reply-To: References: Message-ID: <0A9F39E6-F055-4713-A9BD-488CA6A16A07@yahoo.com> Mesh cassettes are known to hold air pockets in them, so extending your times would only over process your tissue. I would suggest you use sponges, filter paper, or bx bags and use the cassettes you use for your larger specimens until you can get a different cassette. We found in our lab (we didn't have a printer) that the ink will fade if it's not dry enough before blocks are put in formalin container. Same on the slides. Hope this helps. Sent from the iPhone of Kim Tournear On Apr 27, 2011, at 7:21 AM, "Marshall, Kimberly K" wrote: > Morning and Happy Hump day Histo peeps > > Hope everyone is having a fun filled Lab Week, Here is Alaska we are > eating more than usual... > > Just need to ask one more question regarding my evil "nuclear > artifact". We have decided it is a processing issue due to the type of > cassettes we use. In cutting down the processing time, the reagents are > not able to get through the mesh on the cassettes. I have ordered other > kinds but due to limited funds (cant get a new labeler) and even with > the special pens for writing on cassettes and slides the hand written > numbers are fading, I cant use them. My next thought is to lengthen my > biopsy run. Would you folks out there share your biopsy run times with > me? I don't want to go back to having the over processing issues with > the biopsies that occur in the 12 hour, but maybe adding time would help > here. > > Thanks in advance and again hope everyone is having a great Lab week. > > Kim > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Wed Apr 27 08:14:48 2011 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Apr 27 08:15:15 2011 Subject: [Histonet] slide stainer repair Message-ID: Our slide stainer died this morning and I was wondering if anyone knows of a company that repairs the Zeiss HMS Programmable Slide Stainer? It is over 20 years old and may be beyond repair. I am in Central Pennsylvania. Thanks Roberta Horner HT/HTL Animal Diagnostic Lab Pennsylvania State University From JWeems <@t> sjha.org Wed Apr 27 08:53:28 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Apr 27 08:53:36 2011 Subject: [Histonet] Biopsy Run In-Reply-To: <0A9F39E6-F055-4713-A9BD-488CA6A16A07@yahoo.com> References: <0A9F39E6-F055-4713-A9BD-488CA6A16A07@yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F4BC1AD@CHEXCMS10.one.ads.che.org> And one more thing.... Soak the sponges in formalin... j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yahoo Sent: Wednesday, April 27, 2011 08:56 To: Marshall, Kimberly K Cc: Histonet Subject: Re: [Histonet] Biopsy Run Mesh cassettes are known to hold air pockets in them, so extending your times would only over process your tissue. I would suggest you use sponges, filter paper, or bx bags and use the cassettes you use for your larger specimens until you can get a different cassette. We found in our lab (we didn't have a printer) that the ink will fade if it's not dry enough before blocks are put in formalin container. Same on the slides. Hope this helps. Sent from the iPhone of Kim Tournear On Apr 27, 2011, at 7:21 AM, "Marshall, Kimberly K" wrote: > Morning and Happy Hump day Histo peeps > > Hope everyone is having a fun filled Lab Week, Here is Alaska we are > eating more than usual... > > Just need to ask one more question regarding my evil "nuclear > artifact". We have decided it is a processing issue due to the type > of cassettes we use. In cutting down the processing time, the > reagents are not able to get through the mesh on the cassettes. I > have ordered other kinds but due to limited funds (cant get a new > labeler) and even with the special pens for writing on cassettes and > slides the hand written numbers are fading, I cant use them. My next > thought is to lengthen my biopsy run. Would you folks out there share > your biopsy run times with me? I don't want to go back to having the > over processing issues with the biopsies that occur in the 12 hour, > but maybe adding time would help here. > > Thanks in advance and again hope everyone is having a great Lab week. > > Kim > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From nicole <@t> dlcjax.com Wed Apr 27 09:12:43 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Apr 27 09:12:54 2011 Subject: [Histonet] Two Questions Message-ID: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> 1st. Does anyone know if there is a rule or law that states a lab must have a door? 2nd Does anyone know how a TC only lab would do profiency testing on H&E slides. They do not have physician on staff and no microscope. Does the company that is reading PC lab do profiency testing on the QC slides and share results with the TC side? Any thoughts would be appreciated. Nicole Tatum HT, ASCP From nicole <@t> dlcjax.com Wed Apr 27 09:19:12 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Apr 27 09:19:29 2011 Subject: [Histonet] Biopsy Run In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164081F4BC1AD@CHEXCMS10.one.ads.che.org> References: <0A9F39E6-F055-4713-A9BD-488CA6A16A07@yahoo.com> <92AD9B20A6C38C4587A9FEBE3A30E164081F4BC1AD@CHEXCMS10.one.ads.che.org> Message-ID: <3456.208.62.167.196.1303913952.squirrel@webmail.realpages.com> I use the sponges and I keep them in a water so the formalin can penetrate when placed in the processor. Make sure when you put the sponges in the water that you mash them to get out the air bubbles. I use water just in cause my little bowl spills. If it was in formalin I would have to break down my whole grossing station and turn on hood. You can also use kim wipes. Just cut you some squares and place tissue in middle then fold and put in cassette. Hope this helps. Nicole And one more thing.... Soak the sponges in formalin... j > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yahoo > Sent: Wednesday, April 27, 2011 08:56 > To: Marshall, Kimberly K > Cc: Histonet > Subject: Re: [Histonet] Biopsy Run > > Mesh cassettes are known to hold air pockets in them, so extending your > times would only over process your tissue. > > I would suggest you use sponges, filter paper, or bx bags and use the > cassettes you use for your larger specimens until you can get a different > cassette. > > We found in our lab (we didn't have a printer) that the ink will fade if > it's not dry enough before blocks are put in formalin container. Same on > the slides. > > Hope this helps. > > Sent from the iPhone of Kim Tournear > > On Apr 27, 2011, at 7:21 AM, "Marshall, Kimberly K" > wrote: > >> Morning and Happy Hump day Histo peeps >> >> Hope everyone is having a fun filled Lab Week, Here is Alaska we are >> eating more than usual... >> >> Just need to ask one more question regarding my evil "nuclear >> artifact". We have decided it is a processing issue due to the type >> of cassettes we use. In cutting down the processing time, the >> reagents are not able to get through the mesh on the cassettes. I >> have ordered other kinds but due to limited funds (cant get a new >> labeler) and even with the special pens for writing on cassettes and >> slides the hand written numbers are fading, I cant use them. My next >> thought is to lengthen my biopsy run. Would you folks out there share >> your biopsy run times with me? I don't want to go back to having the >> over processing issues with the biopsies that occur in the 12 hour, >> but maybe adding time would help here. >> >> Thanks in advance and again hope everyone is having a great Lab week. >> >> Kim >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dlschneider <@t> gmail.com Wed Apr 27 09:24:31 2011 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Wed Apr 27 09:24:39 2011 Subject: [Histonet] Two Questions In-Reply-To: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> References: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> Message-ID: On 4/27/11, Nicole Tatum wrote: > 1st. Does anyone know if there is a rule or law that states a lab must > have a door? I suspect that it's not explicitly spelled out, but I'd caution you, if you build a lab without doors, you have to make provisions to provide food and water for the HT's imprisoned inside. It's easier to put in doors. DLS From MSHERWOOD <@t> PARTNERS.ORG Wed Apr 27 09:27:47 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Apr 27 09:27:56 2011 Subject: [Histonet] Two Questions In-Reply-To: References: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB56CB@PHSXMB30.partners.org> I believe it is a fire law. Even floors that are open, have to have a door at some end near the elevators. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Wednesday, April 27, 2011 10:25 AM To: Nicole Tatum Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Questions On 4/27/11, Nicole Tatum wrote: > 1st. Does anyone know if there is a rule or law that states a lab must > have a door? I suspect that it's not explicitly spelled out, but I'd caution you, if you build a lab without doors, you have to make provisions to provide food and water for the HT's imprisoned inside. It's easier to put in doors. DLS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From katelin09htl <@t> gmail.com Wed Apr 27 09:28:15 2011 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Wed Apr 27 09:28:23 2011 Subject: [Histonet] Two Questions In-Reply-To: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> References: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> Message-ID: Hi Nicole, You must get a microscope. Spend $600, get a teaching binocular one. You absolutely can not have a histology lab without a microscope. You must be able to check the quality of your staining and cutting. That being said, the lab that does the PC component should also do QC on your slides. If there are major issues they should address them right away, otherwise, you can set up a QC Form that they fill out quarterly to assure your slides are meeting their standards. -- Katelin Lester, HTL MedSurg Pathology Associates, Inc. (503) 443-2157 On Wed, Apr 27, 2011 at 7:12 AM, Nicole Tatum wrote: > 1st. Does anyone know if there is a rule or law that states a lab must > have a door? > > > > 2nd Does anyone know how a TC only lab would do profiency testing on > H&E slides. They do not have physician on staff and no microscope. Does > the company that is reading PC lab do profiency testing on the QC slides > and share results with the TC side? > > Any thoughts would be appreciated. > > Nicole Tatum HT, ASCP > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sgoebel <@t> mirnarx.com Wed Apr 27 09:29:30 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Apr 27 09:29:36 2011 Subject: [Histonet] Two Questions In-Reply-To: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> References: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> Message-ID: Usually I think it has to have a door for fire purposes. Especially since there are so many flammable chemicals in the lab. I could be wrong? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Wednesday, April 27, 2011 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two Questions 1st. Does anyone know if there is a rule or law that states a lab must have a door? 2nd Does anyone know how a TC only lab would do profiency testing on H&E slides. They do not have physician on staff and no microscope. Does the company that is reading PC lab do profiency testing on the QC slides and share results with the TC side? Any thoughts would be appreciated. Nicole Tatum HT, ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Apr 27 09:32:22 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Apr 27 09:32:27 2011 Subject: [Histonet] Two Questions In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB56CB@PHSXMB30.partners.org> References: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB56CB@PHSXMB30.partners.org> Message-ID: Aren't you required to have the lab under negative pressure with respect to the corridor? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, April 27, 2011 10:28 AM To: Daniel Schneider; Nicole Tatum Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two Questions I believe it is a fire law. Even floors that are open, have to have a door at some end near the elevators. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Wednesday, April 27, 2011 10:25 AM To: Nicole Tatum Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Questions On 4/27/11, Nicole Tatum wrote: > 1st. Does anyone know if there is a rule or law that states a lab must > have a door? I suspect that it's not explicitly spelled out, but I'd caution you, if you build a lab without doors, you have to make provisions to provide food and water for the HT's imprisoned inside. It's easier to put in doors. DLS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Wed Apr 27 10:30:13 2011 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Wed Apr 27 10:30:30 2011 Subject: [Histonet] Peloris Processing Message-ID: <8CDD30723167708-1848-117BC@webmail-m062.sysops.aol.com> Has anyone had issues with processing xylene free on the Peloris? If so, can you share them with me? Has anyone used Leica's xylene substitute Sub-X during processing? Thank you, Ann From rjbuesa <@t> yahoo.com Wed Apr 27 10:42:03 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 27 10:42:08 2011 Subject: [Histonet] Biopsy Run In-Reply-To: Message-ID: <847003.80395.qm@web65708.mail.ac4.yahoo.com> I am afraid your "decision" is wrong. "Empty nuclei" appears when the water between the section and the slide is not completely removed and "dissolves" the nuclear contents while drying and no staining after wards. Check the sections draining for this artifact. Ren? J. --- On Wed, 4/27/11, Marshall, Kimberly K wrote: From: Marshall, Kimberly K Subject: [Histonet] Biopsy Run To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 27, 2011, 8:21 AM Morning and Happy Hump day Histo peeps ? Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... ? Just need to ask one more question regarding my evil "nuclear artifact".? We have decided it is a processing issue due to the type of cassettes we use.? In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes.? I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them.? My next thought is to lengthen my biopsy run.? Would you folks out there share your biopsy run times with me?? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here.? Thanks in advance and again hope everyone is having a great Lab week. Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Apr 27 10:46:07 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 27 10:46:10 2011 Subject: [Histonet] Peloris Processing In-Reply-To: <8CDD30723167708-1848-117BC@webmail-m062.sysops.aol.com> Message-ID: <136220.29426.qm@web65703.mail.ac4.yahoo.com> It is better to dehydrate with 2-propanol and go from 2-propanol to paraffin wax than using a "xylene substitute". Ren? J. --- On Wed, 4/27/11, thisisann@aol.com wrote: From: thisisann@aol.com Subject: [Histonet] Peloris Processing To: histonet@lists.utsouthwestern.edu Cc: ofuks@bioreference.com Date: Wednesday, April 27, 2011, 11:30 AM Has anyone had issues with processing xylene free on the Peloris?? If so, can you share them with me?? Has anyone used Leica's xylene substitute Sub-X during processing?? Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Wed Apr 27 11:08:14 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Apr 27 11:08:08 2011 Subject: [Histonet] MSH 2011 Symposium References: <631746.44415.qm@web65716.mail.ac4.yahoo.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B37041083D6@giamail2.Gia.com> If anyone is interested in attending the annual Mississippi Society for Histotechnology Symposium June 24-26th in Hattiesburg, MS, please contact me directly and I can email you a program! We'd love to have you :) Amber McKenzie, BS, HT(ASCP) MSH Secretary From nicole <@t> dlcjax.com Wed Apr 27 12:30:05 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Apr 27 12:30:15 2011 Subject: [Histonet] Two Questions In-Reply-To: References: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> Message-ID: <4091.208.62.167.196.1303925405.squirrel@webmail.realpages.com> Thank you everyone for the input. Nicole Tatum HT(ASCP) Hi Nicole. > > I think that whether or not a door is required, may be up to the fire > department, who should come and inspect your laboratory since you will be > dealing with highly flammable materials. > > Also, in my opinion, even a technical only laboratory should have a > microscope if for no other reason than to review the staining results to > ensure that the work being performed is of the highest quality. > > Good luck and best wishes, > > Debbie Siena HT(ASCP)QIHC > Technical Manager | StatLab Medical Products > Direct: 972-436-1010? x229 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole > Tatum > Sent: Wednesday, April 27, 2011 9:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Two Questions > > 1st. Does anyone know if there is a rule or law that states a lab must > have a door? > > > > 2nd Does anyone know how a TC only lab would do profiency testing on > H&E slides. They do not have physician on staff and no microscope. Does > the company that is reading PC lab do profiency testing on the QC slides > and share results with the TC side? > > Any thoughts would be appreciated. > > Nicole Tatum HT, ASCP > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From micropathlabs <@t> yahoo.com Wed Apr 27 12:31:32 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Wed Apr 27 12:31:36 2011 Subject: [Histonet] Problem with IMEB Message-ID: <628183.1186.qm@web161705.mail.bf1.yahoo.com> Hi all. Has anyone had difficulties in purchasing?refurbished equipment?from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started?about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the?status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now,?IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear?of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had?a similar experience with them??? Thanks for lending an ear (or many). ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From jshelley <@t> sanfordburnham.org Wed Apr 27 12:54:37 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Wed Apr 27 12:54:42 2011 Subject: [Histonet] Problem with IMEB In-Reply-To: <628183.1186.qm@web161705.mail.bf1.yahoo.com> References: <628183.1186.qm@web161705.mail.bf1.yahoo.com> Message-ID: Hi Sheila, I have not worked with this company in the past but have know about them for many years. I was wondering if you tried talking with John O'Brien the owner of the company. He attends many of the histology meetings and I am sure he would not appreciate his company getting any bad publicity through Histonet. Here is a URL about him and maybe you can use his tag line to your benefit since it reads, "We offer you, the customer, 100% product satisfaction." Would love to know what the outcome is. Kind Regards! ? John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 27, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with IMEB Hi all. Has anyone had difficulties in purchasing?refurbished equipment?from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started?about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the?status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now,?IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear?of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had?a similar experience with them??? Thanks for lending an ear (or many). ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Wed Apr 27 12:57:27 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Apr 27 12:57:40 2011 Subject: [Histonet] Problem with IMEB In-Reply-To: <628183.1186.qm@web161705.mail.bf1.yahoo.com> References: <628183.1186.qm@web161705.mail.bf1.yahoo.com> Message-ID: <4443.208.62.167.196.1303927047.squirrel@webmail.realpages.com> I recently used IMEB for the first time and have had a wonderful experience. My rep Margaret has gone above and beyond to satisfy all my questions and needs. She was always very deligant in getting answers to my questions. The poor women probably had to do four or five quotes before we finally hashed it all out. I have used Belair and Pacific in the past. But, I would give my order to Margaret anytime. Nicole Tatum HT(ASCP) Hi all. > Has anyone had difficulties in purchasing?refurbished equipment?from IMEB? > I have been trying to purchase a T2000 ThinPrep processor and sent 50% > payment > with net due in 30 days. This transaction started?about a month ago and > they've > had our partial payment for more than 3 weeks but had not shipped the > instrument. I've repeatedly called to get the?status of shipment but have > gotten > the run around with checking our credit references. This transaction has > left a > very bad taste in our mouths so I proceeded to request our payment back > and > cancel the order yesterday. Now,?IMEB says they'll return our deposit > minus a > 20% restocking fee for an item that never even left their facility. > > I was surprised to hear?of this from IMEB given I'd heard they were a > reputable > company. My opinion has definetly changed! Just wondered if anyone else > has > had?a similar experience with them??? > > Thanks for lending an ear (or many). > ? > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jshelley <@t> sanfordburnham.org Wed Apr 27 13:01:46 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Wed Apr 27 13:01:51 2011 Subject: [Histonet] Problem with IMEB In-Reply-To: References: <628183.1186.qm@web161705.mail.bf1.yahoo.com> Message-ID: Oops!! Forgot the URL http://www.imebinc.com/aboutimeb.php Here you go! Kind Regards! ? John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, April 27, 2011 1:55 PM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Problem with IMEB Hi Sheila, I have not worked with this company in the past but have know about them for many years. I was wondering if you tried talking with John O'Brien the owner of the company. He attends many of the histology meetings and I am sure he would not appreciate his company getting any bad publicity through Histonet. Here is a URL about him and maybe you can use his tag line to your benefit since it reads, "We offer you, the customer, 100% product satisfaction." Would love to know what the outcome is. Kind Regards! ? John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 27, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with IMEB Hi all. Has anyone had difficulties in purchasing?refurbished equipment?from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started?about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the?status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now,?IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear?of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had?a similar experience with them??? Thanks for lending an ear (or many). ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Wed Apr 27 13:01:45 2011 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Apr 27 13:01:55 2011 Subject: [Histonet] MTS/Verhoeff's Stain Message-ID: Does anyone have a procedure for a combination MTS/VG elastic stain that they would be willing to share? Diana From micropathlabs <@t> yahoo.com Wed Apr 27 13:06:52 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Wed Apr 27 13:06:55 2011 Subject: [Histonet] Problem with IMEB In-Reply-To: References: <628183.1186.qm@web161705.mail.bf1.yahoo.com> Message-ID: <511894.51834.qm@web161718.mail.bf1.yahoo.com> John, Thanks for the information. I actually asked for the owner today, he'd previously been out of?the country, but was still unavailable. I've repeatedly told them we'd?done?everything they'd requested but they were still sitting on our money and the instrument. It wasn't until I threatened to cancel that they offered to send the instrument?to us immediately.?Poor business?practice as far as I'm concerned and I'm now very leary of the remainder of their business practices?(service, etc...). I hope they come through with a complete refund of our money. I'll be sure to let everyone know how it turns out. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? ________________________________ From: John Shelley To: Sheila Haas ; "histonet@lists.utsouthwestern.edu" Sent: Wed, April 27, 2011 2:01:46 PM Subject: RE: [Histonet] Problem with IMEB Oops!! Forgot the URL http://www.imebinc.com/aboutimeb.php Here you go! Kind Regards! ? John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email:? jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, April 27, 2011 1:55 PM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Problem with IMEB Hi Sheila, I have not worked with this company in the past but have know about them for many years. I was wondering if you tried talking with John O'Brien the owner of the company. He attends many of the histology meetings and I am sure he would not appreciate his company getting any bad publicity through Histonet. Here is a URL about him and maybe you can use his tag line to your benefit since it reads, "We offer you, the customer, 100% product satisfaction." Would love to know what the outcome is. Kind Regards! ? John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email:? jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 27, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with IMEB Hi all. Has anyone had difficulties in purchasing?refurbished equipment?from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started?about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the?status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now,?IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear?of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had?a similar experience with them??? Thanks for lending an ear (or many). ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Wed Apr 27 13:09:43 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Apr 27 13:09:46 2011 Subject: [Histonet] Problem with IMEB In-Reply-To: <628183.1186.qm@web161705.mail.bf1.yahoo.com> References: <628183.1186.qm@web161705.mail.bf1.yahoo.com> Message-ID: I have had excellent luck and service with them. Call Denise deVines she is super nice and helpful =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 27, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with IMEB Hi all. Has anyone had difficulties in purchasing?refurbished equipment?from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started?about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the?status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now,?IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear?of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had?a similar experience with them??? Thanks for lending an ear (or many). ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dunatrsd <@t> sbcglobal.net Wed Apr 27 13:17:03 2011 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Wed Apr 27 13:17:07 2011 Subject: [Histonet] Temporary (8 months) Histology position in San Diego In-Reply-To: References: <628183.1186.qm@web161705.mail.bf1.yahoo.com> Message-ID: <982641.24000.qm@web83908.mail.sp1.yahoo.com> Hi Everyone, There is a position for a ?Histotechnologist at one of the major Pharmaceutical companies located in the Torrey Pines/SDSU/La Jolla?area. Here is the job description and if anyone knows of someone that is interested please have them contact me. ? Thank you ? Dusko Trajkovic 858-638-6202 ? Temporary Histology position: ? Position will be for approximately 8 months.? It is approved for 40 hours per week but there is some flexibility and as long as there is overlap with?a lead histotech the time of day could be flexed.? It is?an ?associate scientist III level and will be competitive.? This would be a good opportunity for someone who may want to see what industry is like and to also get a crash course in IHC as we do a lot here.? It is all non-GLP.? ? Specific responsibilities in assisting the histology lab may include: ? ? ? ????????? Trim formalin fixed rat/mouse organs ? ????????? Process and embed tissue specimens ? ????????? Cut frozen? and paraffin (mainly) sections ? ????????? Perform H&E stains and special stains as needed ? ????????? Perform IHC staining manually or by autostainer ? ????????? Label cassettes and slides ? ????????? Change reagents in tissue processer and autostainer ? ????????? Maintain tissue blocks and slide inventories ? ? ? ? ? Requirements: ? ???? BS in a scientific discipline ? ???? 5 years in histology ? ???? Tissue processor (VIP), coverslipper, microtome, cryostat, autostainer (DAKO? ? ????? and Sakura) experience ? ???? Skills with frozen and paraffin sectioning, H&E stains and IHC stains ? ???? HT preferred From rjbuesa <@t> yahoo.com Wed Apr 27 13:18:45 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 27 13:18:48 2011 Subject: [Histonet] Problem with IMEB In-Reply-To: <628183.1186.qm@web161705.mail.bf1.yahoo.com> Message-ID: <553662.18025.qm@web65710.mail.ac4.yahoo.com> As with any other commercial transaction gone "sour", contact the Better Business Bureau on the area the company operates, and present a formal and documented complaint. Ren? J. --- On Wed, 4/27/11, Sheila Haas wrote: From: Sheila Haas Subject: [Histonet] Problem with IMEB To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 27, 2011, 1:31 PM Hi all. Has anyone had difficulties in purchasing?refurbished equipment?from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started?about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the?status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now,?IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear?of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had?a similar experience with them??? Thanks for lending an ear (or many). ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mweirauch <@t> crittenton.com Wed Apr 27 14:43:55 2011 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Wed Apr 27 14:45:24 2011 Subject: [Histonet] Searching for someone Message-ID: I am looking for Cody Best. Does anyone have a way to get hold of him? Thanks in advance! From rjr6 <@t> psu.edu Wed Apr 27 14:52:36 2011 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Apr 27 14:52:59 2011 Subject: [Histonet] Automated slide stainer Message-ID: I just got a pleasant surprise. After asking about repair for my dead stainer I was just told to get quotes for a new stainer ASAP. I need help I haven't looked at stainers for a long time. What is everyone using and how do you like it? Ours is a small lab average 50 slides per day seldom over 80. My old stainer did 50 slides a rack and that is a good capacity for us. Thanks for the information on repairs that I received. Roberta Horner HT/HTL Pennsylvania State University Animal Diagnostic Lab From rjbuesa <@t> yahoo.com Wed Apr 27 15:02:06 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 27 15:02:10 2011 Subject: [Histonet] Automated slide stainer In-Reply-To: Message-ID: <960743.53388.qm@web65714.mail.ac4.yahoo.com> Check Sakura stainers Ren? J. --- On Wed, 4/27/11, Roberta Horner wrote: From: Roberta Horner Subject: [Histonet] Automated slide stainer To: "Histonet" Date: Wednesday, April 27, 2011, 3:52 PM I just got a pleasant surprise.? After asking about repair for my dead stainer I was just told to get quotes for a new stainer ASAP. I need help I haven't looked at stainers for a long time.? What is everyone using and how do you like it?? Ours is a small lab average 50 slides per day seldom over 80.? My old stainer did 50 slides a rack and that is a good capacity for us. Thanks for the information on repairs that I received. Roberta Horner HT/HTL Pennsylvania State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Apr 27 15:48:10 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Apr 27 15:48:18 2011 Subject: [Histonet] Two Questions Message-ID: Hi, This is probably totally not helpful and I apologize in advance, but do you climb in through the window? I'm thinking the histology lab meets Dukes of Hazard! Sorry about the giggles at your expense but YEE HAW that's funny! Can't wait to see Daisy Duke in a lab coat & cut offs! Amos On Wed, Apr 27, 2011 at 10:20 AM, wrote: > Message: 24 > Date: Wed, 27 Apr 2011 10:12:43 -0400 (EDT) > From: "Nicole Tatum" > Subject: [Histonet] Two Questions > To: histonet@lists.utsouthwestern.edu > Message-ID: > <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> > Content-Type: text/plain;charset=iso-8859-1 > > 1st. Does anyone know if there is a rule or law that states a lab must > have a door? > > > > 2nd Does anyone know how a TC only lab would do profiency testing on > H&E slides. They do not have physician on staff and no microscope. Does > the company that is reading PC lab do profiency testing on the QC slides > and share results with the TC side? > > Any thoughts would be appreciated. > > Nicole Tatum HT, ASCP > From POWELL_SA <@t> mercer.edu Wed Apr 27 15:52:55 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Apr 27 15:53:16 2011 Subject: [Histonet] Diff-Quik stain Message-ID: <9BF995BC0E47744E9673A41486E24EE238D54B9BA5@MERCERMAIL.MercerU.local> Hi Guys, I am looking for diff-quick stain kits that are economical. My pathologist is contemplating another medical mission trip to Haiti and I need to locate a kit that she can take with her for blood smears. I have found a few upwards of $600 but she does not need a gallon of each of the solutions. If you know of a supplier of smaller volumes or even if you have protocols where you make your own, please share. Vendors comments are welcome if you contact me personally, please do not flood histonet with ads. Thanks in advance. Shirley Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From Lynn.Burton <@t> Illinois.gov Wed Apr 27 16:04:24 2011 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Apr 27 16:05:05 2011 Subject: [Histonet] RE: Diff-Quik stain In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238D54B9BA5@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE238D54B9BA5@MERCERMAIL.MercerU.local> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00231C837BA@IL084EXMBX214.illinois.gov> Fisher has a Hema 3 System in 500mL bottles for $144.75 that I use. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell [POWELL_SA@mercer.edu] Sent: Wednesday, April 27, 2011 3:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diff-Quik stain Hi Guys, I am looking for diff-quick stain kits that are economical. My pathologist is contemplating another medical mission trip to Haiti and I need to locate a kit that she can take with her for blood smears. I have found a few upwards of $600 but she does not need a gallon of each of the solutions. If you know of a supplier of smaller volumes or even if you have protocols where you make your own, please share. Vendors comments are welcome if you contact me personally, please do not flood histonet with ads. Thanks in advance. Shirley Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Apr 27 16:08:13 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Apr 27 16:08:17 2011 Subject: [Histonet] Diff-Quik stain In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238D54B9BA5@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE238D54B9BA5@MERCERMAIL.MercerU.local> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB56D0@PHSXMB30.partners.org> Shirley, I'm not sure if this is what you want, but we just ordered Hema-Diff Rapid Differential Stain(a modified Giemsa/Romanowsky) from StatLabs. Consists of 3 solutions: Soltn 1: Fixative, Soltn 2: Xanthene Dye and Soltn 3: Thiazine Dye. They sell it in gallon size and pint size. The gallon size is $35.00 and the pint size (16oz) is $10.00. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, April 27, 2011 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diff-Quik stain Hi Guys, I am looking for diff-quick stain kits that are economical. My pathologist is contemplating another medical mission trip to Haiti and I need to locate a kit that she can take with her for blood smears. I have found a few upwards of $600 but she does not need a gallon of each of the solutions. If you know of a supplier of smaller volumes or even if you have protocols where you make your own, please share. Vendors comments are welcome if you contact me personally, please do not flood histonet with ads. Thanks in advance. Shirley Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From madary <@t> verizon.net Wed Apr 27 16:53:46 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Wed Apr 27 16:49:22 2011 Subject: [Histonet] power outages for processors Message-ID: <202653717.2995542.1303941226997.JavaMail.root@vznit170064> here are usually UPS(uninterrupted Power suppy) batteries available f or just a couple of hundered dollars. The unit plugs into the wall and the Nick(Rocky) Mad Joni Madary, PhD(in life) Apr 25, 2011 12:0 Send Histonet histonet@lists.utsouthwestern.edu To http://lists.uts or, via email, send a message histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lis When replying, please edit your Subject line s specific than "Re: Contents of Histonet digest..." < 1. Histotech position in Irving, Texas (Gloria Co 2. RE: Processors and power outages (Amber McKenzie) 3. FDA appro 4. Happy National Medical Laboratory Pro (Brian- Prometheus) ------------------------ ---------------------------------------------- Message: 1 Date: S From: Gloria Cole Subject: [Histonet] Histotech position in Irving, Texas To: histonet@l Message-ID: <55D857E3-9B4D-4278-9568-E1836A8C Content-Type: text/plain; charset=us-ascii Hel Just wanted to let everyone know I have one and may have another Histotech/ Grossing tech position open in our GI lab in Irving, Texas. The day shift. If you a contact me for more details. Thanks, Gloria Cole BS HT (ASCP) NuePath Supervisor 972-489-2071 gcole@nueterrapathology.com ------------------- Message: 2 Date: Mon, 25 Apr 2011 08:13:07 -0500 F Subject: RE: [Hist To: "Heather R" ,< Message-ID: <03C921A1EAF7F5 Content-Type: text/plain Back-up battery -----Original Messag From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- bounces@lists.utsouthwestern.edu] On Behalf Of Heather R Sent: Saturday, To: histonet@lists.utsouthwestern.edu Subject Wondering what the labs wh doing with thier processors for pow lab and want to address this issue. Th H. _______________________________________________ Histonet m Histonet@lists.utsouthwestern.edu http://lists.utsouthwes ------------------------- Message: 3 Date: Mon, 25 Apr 2011 09:02:10 -0500 From: " Subject: [Histonet] FDA approved I To: "histonet@lists.utsouthwestern.edu" Message-ID: <24A4826E8EF0964D86BC5317306F58A55D F526D86C@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset=" Question - I am trying to figure why we should switch to a approved IVD antibody for Cd117 from the current IVD antibody we use are not FDA app have to do if an antibody is questioned about the true advantage The cost of course for an FDA approved antibody in comparison to other IVD antibodies that are not FDA a (as much as five times as much). In this case I am trying to figur e out what is so special about Cd117. If someone could enlighten me Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medic 217-788-4046 ________________________________ Th information in is protected by law. If y should delete this message. Any disc distribution of this message, or the taking of any acti on it, is strictly prohibited. ------------------------ Message: 4 Date: Mon, 25 Apr 2011 10:50:39 -0400 From: Subject: [Histonet] Professionals Week To: Message-ID: <00aa01cc0358$281e1fd0$785a5f70 Content-Type: text/plain; charset="us-ascii" National celebration of the m vital role i from April< NMLPW is a chance for medica their professionalism and be recogni use this time to inform and educate m about the medical laboratory. It's esti that up to 70 percent of medical decisions are made based on t test results generated by these highly-skilled professionals. Thanks to the 265,000 medical laboratory professionals and 15,000board-certified Pathologists who play a vital role in every aspect of hea care!!! Happy Lab Professionals Week! Prometheus Healthcare ( www.prometheushealthcare.com) is committed to connecting dedicated lab Prometheus Healthcare Office 301-693-90 Cell 301-605-5450 Fax 301-368-2478 info@prometheush www.prometheushe *** Stay up to date on the newest positions and healthc nationwide on Twitter!*** http://twitter.com/PrometheusBlog ----------------------- _______________________________________________ Histonet Histonet@lists.utsouthwestern.edu http://lists.utsouthwe End of Histonet Digest, Vol 89, **************************************** From rbutler <@t> ameripath.com Wed Apr 27 16:54:17 2011 From: rbutler <@t> ameripath.com (Butler, Roszetta) Date: Wed Apr 27 16:54:26 2011 Subject: [Histonet] Re: C4d stain for IF Message-ID: I'm having trouble with my C4d IF stain. I'm using AbDirect/Serotec, mouse monoclonal diluted 1:500 for 1 hr, and the Vector is AbDirect/Serotec diluted 1:25 for ? hour. This is not working and I've tried different dilutions with no success. Can anyone share their protocol? Rosie Butler, AmeriPath/3000 United Founders Blvd./Suite 234/Oklahoma City, OK 73112/405.842.7575 - Office 405.650.8921 - Cell/405.841.2002 - Fax CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From AnthonyH <@t> chw.edu.au Wed Apr 27 18:49:41 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Apr 27 18:49:57 2011 Subject: [Histonet] RE: Biopsy Run In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715718852E92@xmdb02.nch.kids> Increase the fixation time. If they are small biopsies (otherwise why would you be putting them in mesh cassettes), you could easily reduce the processing time to 30 minutes (eg 4 alcohols, 2-3 xylenes, 2-3 waxes) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Wednesday, 27 April 2011 10:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Run Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil "nuclear artifact". We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From hlukey <@t> msn.com Wed Apr 27 19:25:51 2011 From: hlukey <@t> msn.com (Hugh Luk) Date: Wed Apr 27 19:25:57 2011 Subject: [Histonet] Diff-Quik stain In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238D54B9BA5@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE238D54B9BA5@MERCERMAIL.MercerU.local> Message-ID: How about this from PolySciences Inc. $68. http://www.polysciences.com/Core/Display.aspx?pageId=98&categoryId=203&productId=2865 Looks compact and transportable. Too small? > From: POWELL_SA@mercer.edu > To: histonet@lists.utsouthwestern.edu > Date: Wed, 27 Apr 2011 16:52:55 -0400 > Subject: [Histonet] Diff-Quik stain > > Hi Guys, > > I am looking for diff-quick stain kits that are economical. My pathologist is contemplating another medical mission trip to Haiti and I need to locate a kit that she can take with her for blood smears. I have found a few upwards of $600 but she does not need a gallon of each of the solutions. If you know of a supplier of smaller volumes or even if you have protocols where you make your own, please share. > > Vendors comments are welcome if you contact me personally, please do not flood histonet with ads. > > Thanks in advance. > > Shirley > > Shirley A. Powell, HT(ASCP)HTL, QIHC > Technical Director > Histology Curricular Support Laboratory > Mercer University School of Medicine > 1550 College Street > Macon, GA 31207 > 478-301-2374 Lab > 478-301-5489 Fax > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Apr 27 20:47:10 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Apr 27 20:47:14 2011 Subject: [Histonet] Re: Diff-Quik stain Message-ID: Diff-Quik is a trade name - belongs to whatever Scientific Products is called this week. It's often used generically for all two-stain Romanowsky type stains, since there isn't a really satisfactory generic name for them. I've used several generic stains with separate eosin ("xanthine") and blue ("thiazine") dye baths, and generally found them satisfactory. But you do need to try them out for yourself. Bob Richmond Samurai Pathologist Knoxville TN From k84as <@t> yahoo.com Thu Apr 28 03:10:06 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Thu Apr 28 03:10:10 2011 Subject: [Histonet] chicken immunohistochemistry Message-ID: <496385.41591.qm@web112602.mail.gq1.yahoo.com> dear all i'm?looking for vendors or companies that we could order some anti chicken antibodies (primary & secondary). speacially for CD4 & CD8 i'm new in that feild and need your help. ? mohamed ? From histotalk <@t> yahoo.com Thu Apr 28 05:12:07 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu Apr 28 05:12:11 2011 Subject: [Histonet] MSH 2011 Symposium In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B37041083D6@giamail2.Gia.com> References: <631746.44415.qm@web65716.mail.ac4.yahoo.com> <03C921A1EAF7F541B16543F6EC6A4B37041083D6@giamail2.Gia.com> Message-ID: <177783.69813.qm@web120605.mail.ne1.yahoo.com> Hi Amber - I have the MSH?Symposium?scheduled to begin promos on May 8th's HistoTALK. Hopefully, the announcements will help get listeners attention and get them to attend. I'll be sure to mention your email on the show. :- ) Yours, Dave www.HistoTALK.com ________________________________ From: Amber McKenzie Cc: Histonet list serv. Sent: Wed, April 27, 2011 12:08:14 PM Subject: [Histonet] MSH 2011 Symposium If anyone is interested in attending the annual Mississippi Society for Histotechnology Symposium June 24-26th in Hattiesburg, MS, please contact me directly and I can email you a program!? We'd love to have you :) Amber McKenzie, BS, HT(ASCP) MSH Secretary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Thu Apr 28 06:17:14 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Apr 28 06:17:19 2011 Subject: [Histonet] IMEB Message-ID: <967305.23545.qm@web161712.mail.bf1.yahoo.com> Thanks?to everyone?for the feedback on IMEB. It appears?there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From kryan <@t> nfderm.com Thu Apr 28 09:32:22 2011 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Thu Apr 28 09:32:26 2011 Subject: [Histonet] IMEB In-Reply-To: <967305.23545.qm@web161712.mail.bf1.yahoo.com> References: <967305.23545.qm@web161712.mail.bf1.yahoo.com> Message-ID: I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks?to everyone?for the feedback on IMEB. It appears?there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Thu Apr 28 09:46:09 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Apr 28 09:46:14 2011 Subject: [Histonet] IMEB In-Reply-To: References: <967305.23545.qm@web161712.mail.bf1.yahoo.com> Message-ID: I agree Kaye!! IMEB has always gone above and beyond to help me with anything I have ever needed. They even had one of their techs call and help me troubleshoot some equipment that wasn't even theirs! I can never say enough awesome things about Denise either!! She is AWESOME!! Keeps in contact and tries to help out in any way she can!! I bought a used processor from them and a few months later it had an issue. They sent someone to my lab (on their dime by the way) who fixed it on the spot! From the time I called to the time it was fixed was less than 2 days!! It was an old VIP and the repair tech even made his own diagnostic equipment and repair parts! I would recommend this company to anyone (and have!!). It is a shame that one person's bad experience has been broadcast over the histonet for the world to see. With anything in this world there is always going to be one person who has a bad taste in their mouth about something. Because people always yell out the bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY!!!! I will now step down off my box =) Have a Happy Thursday Histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Thursday, April 28, 2011 9:32 AM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IMEB I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks?to everyone?for the feedback on IMEB. It appears?there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Thu Apr 28 09:48:00 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Apr 28 09:48:04 2011 Subject: [Histonet] IMEB In-Reply-To: References: <967305.23545.qm@web161712.mail.bf1.yahoo.com> Message-ID: <829976.69833.qm@web161717.mail.bf1.yahoo.com> Kaye, I was telling of my situation and asked if others?had recent problems. I received many responses with two being positive and?most of the others negative (a couple were offering suggestions on how to handle). I have heard this company was very reputable which is why I chose to do business with them in the first place but have since heard?that recently things have?been quite rocky. I do feel, at this point, that others should be leary before ordering from this company. I'd hate for someone else to be in the same situation as we are, particularly during these difficult economic times (we are out a large sum of money at this point). I also stated that if the issue is resolved, I would let everyone know but?up until now it has not been. You are right, we did not have an account with IMEB but what was promised in the beginning is not what played out. I have been in this field for a very long time and realize?how the accounting works?but I also realize that a?full refund is not unreasonable given I jumped through every hoop they requested I jump through.?Keep in mind, restocking fees typically occur when items are already shipped or delivered to the customer and they return them. This is not the case here, they have both the item and?our money. Again, if this issue is resolved to my satisfaction, I will gladly let everyone know. Sheila Haas ? ? ________________________________ From: Kaye Ryan To: Sheila Haas ; histonet@lists.utsouthwestern.edu Sent: Thu, April 28, 2011 10:32:22 AM Subject: RE: [Histonet] IMEB I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting.? I have had many excellent transactions with IMEB and would recommend them to anyone.? Their products are trustworthy and their prices are excellent compared to other companies that sell the same products.? I have known the owner and several of the reps for many years and have never heard of an incident like this.? I too, had to put a deposit down when I made my first order because we did not have an established account with them.? I am assuming that you did not have an account already established since they were checking your credit status?? It does take time to fill out the paper work and for them to check your credit references.? It is that way with almost any new company that you place a large order with.? In this day and time "trust" is a hard thing to come by on a new account.? It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them".? This can be interpreted by some to mean that there have been many negative interactions so stay clear of them.? Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks?to everyone?for the feedback on IMEB. It appears?there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Apr 28 09:48:01 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu Apr 28 09:48:47 2011 Subject: [Histonet] IMEB In-Reply-To: References: <967305.23545.qm@web161712.mail.bf1.yahoo.com> Message-ID: It would clear things up quite a bit if they would simply return her call. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Thursday, April 28, 2011 10:46 AM To: kryan@nfderm.com; micropathlabs@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IMEB I agree Kaye!! IMEB has always gone above and beyond to help me with anything I have ever needed. They even had one of their techs call and help me troubleshoot some equipment that wasn't even theirs! I can never say enough awesome things about Denise either!! She is AWESOME!! Keeps in contact and tries to help out in any way she can!! I bought a used processor from them and a few months later it had an issue. They sent someone to my lab (on their dime by the way) who fixed it on the spot! From the time I called to the time it was fixed was less than 2 days!! It was an old VIP and the repair tech even made his own diagnostic equipment and repair parts! I would recommend this company to anyone (and have!!). It is a shame that one person's bad experience has been broadcast over the histonet for the world to see. With anything in this world there is always going to be one person who has a bad taste in their mouth about something. Because people always yell out the bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY!!!! I will now step down off my box =) Have a Happy Thursday Histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Thursday, April 28, 2011 9:32 AM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IMEB I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks?to everyone?for the feedback on IMEB. It appears?there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 28 09:51:39 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 28 09:51:43 2011 Subject: [Histonet] IMEB In-Reply-To: Message-ID: <119316.93664.qm@web65711.mail.ac4.yahoo.com> Kaye: In no way this posting is aimed at "roasting" yours, not at all, it is just to explain MY philosophy when participating in HistoNet. When I read a posting first I consider if I have something useful to contribute and if that is the case I post my answer and I really do not care if it will follow a "consensus", it is my answer based on my experience and from the start I consider that I may be wrong or that others' answers will reflect reality in a better way. The fact that you may have a good commercial experience with IMEB in absolutely no way excludes the possibility that others' experiences could have been disastrous. Additionally, it does not really matter how others have related to this company if the one posting the initial query has had a bad experience. Summing up do as I do: post your answer if you consider will help, and absolutely disregard what others may think of it, and never try to impose your criterion. Ren? J. --- On Thu, 4/28/11, Kaye Ryan wrote: From: Kaye Ryan Subject: RE: [Histonet] IMEB To: "Sheila Haas" , histonet@lists.utsouthwestern.edu Date: Thursday, April 28, 2011, 10:32 AM I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting.? I have had many excellent transactions with IMEB and would recommend them to anyone.? Their products are trustworthy and their prices are excellent compared to other companies that sell the same products.? I have known the owner and several of the reps for many years and have never heard of an incident like this.? I too, had to put a deposit down when I made my first order because we did not have an established account with them.? I am assuming that you did not have an account already established since they were checking your credit status?? It does take time to fill out the paper work and for them to check your credit references.? It is that way with almost any new company that you place a large order with.? In this day and time "trust" is a hard thing to come by on a new account.? It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them".? This can be interpreted by some to mean that there have been many negative interactions so stay clear of them.? Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks?to everyone?for the feedback on IMEB. It appears?there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Apr 28 10:03:43 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Apr 28 10:03:50 2011 Subject: [Histonet] Ventana Probes Message-ID: <4DB9498F.2B7F.00C9.1@geisinger.edu> Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rant....anyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From akbitting <@t> geisinger.edu Thu Apr 28 10:10:45 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Apr 28 10:10:56 2011 Subject: [Histonet] Ventana Probes In-Reply-To: <4DB9498F.2B7F.00C9.1@geisinger.edu> References: <4DB9498F.2B7F.00C9.1@geisinger.edu> Message-ID: <4DB94B35.2B7F.00C9.1@geisinger.edu> Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts? >>> "Angela Bitting" 4/28/2011 11:03 AM >>> Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rant....anyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From rjbuesa <@t> yahoo.com Thu Apr 28 10:13:18 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 28 10:13:21 2011 Subject: [Histonet] Ventana Probes In-Reply-To: <4DB9498F.2B7F.00C9.1@geisinger.edu> Message-ID: <433054.26360.qm@web65712.mail.ac4.yahoo.com> Before adding vodka, take some "sips". Ren? J. --- On Thu, 4/28/11, Angela Bitting wrote: From: Angela Bitting Subject: [Histonet] Ventana Probes To: "histonet" Date: Thursday, April 28, 2011, 11:03 AM Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml).? Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rant....anyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone? 570-214-9634 fax? 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 28 10:17:16 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 28 10:17:19 2011 Subject: [Histonet] Ventana Probes In-Reply-To: <4DB94B35.2B7F.00C9.1@geisinger.edu> Message-ID: <533509.43351.qm@web65712.mail.ac4.yahoo.com> At 100?L per dispense, to do 50 tests you will need 50 x 100?L = 5000 ?L = 5 ml You do need to dilute it Ren? J. --- On Thu, 4/28/11, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Ventana Probes To: "Angela Bitting" , "histonet" Date: Thursday, April 28, 2011, 11:10 AM Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts? >>> "Angela Bitting" 4/28/2011 11:03 AM >>> Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml).? Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rant....anyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone? 570-214-9634 fax? 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Thu Apr 28 11:07:42 2011 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Apr 28 11:07:48 2011 Subject: [Histonet] TNF alpha antibody Message-ID: <62A8156F8071C8439080D626DF8C33A60174E398@wave-mail.7thwave.local> Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue? Any suggestions are greatly appreciated! From liz <@t> premierlab.com Thu Apr 28 11:15:48 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Apr 28 11:15:52 2011 Subject: [Histonet] TNF alpha antibody In-Reply-To: <62A8156F8071C8439080D626DF8C33A60174E398@wave-mail.7thwave.local> Message-ID: We have used the antibody from abcam on FFPE mouse tissue with good success, have not tired it on frozen sections Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, April 28, 2011 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TNF alpha antibody Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue? Any suggestions are greatly appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Apr 28 11:30:28 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Apr 28 11:30:32 2011 Subject: [Histonet] TNF-alpha Message-ID: I was incorrect in my earlier post, we actually use the antibody from Sigma for TNF- alpha, I was thinking of IL-6 that's the antibody we get from abcam Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From foreightl <@t> gmail.com Thu Apr 28 12:15:04 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Thu Apr 28 12:15:08 2011 Subject: [Histonet] Re: C4d stain for IF In-Reply-To: References: Message-ID: Try the C4d directly labeled antibody from alpco www.alpco.com anti human C4d (FITC-Conjugated) Cat #: 04-BI-RC4D-FITC Good luck. On Wed, Apr 27, 2011 at 2:54 PM, Butler, Roszetta wrote: > I'm having trouble with my C4d IF stain. ?I'm using AbDirect/Serotec, mouse monoclonal diluted 1:500 for 1 hr, and the Vector is AbDirect/Serotec ?diluted 1:25 for ? hour. ?This is not working and I've tried different dilutions with no success. ?Can anyone share their protocol? > > Rosie Butler, > AmeriPath/3000 United Founders Blvd./Suite 234/Oklahoma City, OK 73112/405.842.7575 - Office > 405.650.8921?- Cell/405.841.2002 - Fax > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From tpodawiltz <@t> lrgh.org Thu Apr 28 13:18:47 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Apr 28 13:18:55 2011 Subject: [Histonet] IMEB In-Reply-To: <119316.93664.qm@web65711.mail.ac4.yahoo.com> References: <119316.93664.qm@web65711.mail.ac4.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DEBE7C0C@LRGHEXVS1.practice.lrgh.org> Since my Navy time I have had two major careers in my life time. Histology and Sales(17 yrs). My experience gained me this prospective: All companies go through periods were everything they do is done right, then a few key people leave and suddenly they can't get out of their own way. Sometimes they figure it out and get back on track and sometimes they don't and fail. I am not familiar with IMEB at all and cannot comment on their status. I will comment on what I always told my staff. "It is unforgivable not to call a customer back, no matter how bad the news is". "Never lie, that way you don't have to remember what you said the last time you talked to them". "Remember this, a bad experience of any kind with us travels faster that a good one". "Above all never ever lie to me". We had customers that loved us because we did what we said were going to do and others that hated us because sometimes we just could not get out of our own way. By the way, I left my last sales position because of the President of Sales (minor owner), would not call back customers and would downright lie to them. He was later bought out and terminated. Thus my return to Histology. I think the only point I am trying to make is that there is always two sides to the story. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, April 28, 2011 10:52 AM To: Sheila Haas; histonet@lists.utsouthwestern.edu; Kaye Ryan Subject: RE: [Histonet] IMEB Kaye: In no way this posting is aimed at "roasting" yours, not at all, it is just to explain MY philosophy when participating in HistoNet. When I read a posting first I consider if I have something useful to contribute and if that is the case I post my answer and I really do not care if it will follow a "consensus", it is my answer based on my experience and from the start I consider that I may be wrong or that others' answers will reflect reality in a better way. The fact that you may have a good commercial experience with IMEB in absolutely no way excludes the possibility that others' experiences could have been disastrous. Additionally, it does not really matter how others have related to this company if the one posting the initial query has had a bad experience. Summing up do as I do: post your answer if you consider will help, and absolutely disregard what others may think of it, and never try to impose your criterion. Ren? J. --- On Thu, 4/28/11, Kaye Ryan wrote: From: Kaye Ryan Subject: RE: [Histonet] IMEB To: "Sheila Haas" , histonet@lists.utsouthwestern.edu Date: Thursday, April 28, 2011, 10:32 AM I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting.? I have had many excellent transactions with IMEB and would recommend them to anyone.? Their products are trustworthy and their prices are excellent compared to other companies that sell the same products.? I have known the owner and several of the reps for many years and have never heard of an incident like this.? I too, had to put a deposit down when I made my first order because we did not have an established account with them.? I am assuming that you did not have an account already established since they were checking your credit status?? It does take time to fill out the paper work and for them to check your credit references.? It is that way with almost any new company that you place a large order with.? In this day and time "trust" is a hard thing to come by on a new account.? It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them".? This can be interpreted by some to mean that there have been many negative interactions so stay clear of them.? Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks?to everyone?for the feedback on IMEB. It appears?there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From rosenfeldtek <@t> hotmail.com Thu Apr 28 13:38:55 2011 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Thu Apr 28 13:39:00 2011 Subject: [Histonet] Recycling slide brite In-Reply-To: <253407.14943.qm@web65715.mail.ac4.yahoo.com> References: , <253407.14943.qm@web65715.mail.ac4.yahoo.com> Message-ID: Looks like B/R has a gadget that will do it... http://www.solvent--recycling.com/xylene_substitutes.html Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Tue, 26 Apr 2011 12:51:15 -0700 > From: rjbuesa@yahoo.com > To: Histonet@lists.utsouthwestern.edu; dwenzel01@gmail.com > Subject: Re: [Histonet] Recycling slide brite > CC: > > Being a MIXTURE of hydrocarbons, it will crack at different temperatures (separately). You would have to reconstitute it using the same proportions (usually "proprietary"). > Ren? J. > > --- On Tue, 4/26/11, Dawn Herron wrote: > > > From: Dawn Herron > Subject: [Histonet] Recycling slide brite > To: Histonet@lists.utsouthwestern.edu > Date: Tuesday, April 26, 2011, 2:25 PM > > > Hello histonetters. We have made the transition from xylene to using slide > brite in our stainer and for coverslipping and have worked out all the kinks > except one---recycling it successfully. We've been talking to our recycler > manufacturer (B&R) and the manufacturer of slide brite and still haven't had > a successful run. Does anyone out there use this product and recycle it? If > so could you send me your recycling protocol? Any thoughts would be > appreciated. > > Thanks, > Dawn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Thu Apr 28 13:41:45 2011 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Thu Apr 28 13:41:48 2011 Subject: [Histonet] Supplier for human testis control slides? Message-ID: I'm working up a new antibody and human testis is the suggested postive control. Does anyone know of a supplier? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology From Lynne.Bell <@t> cvmc.org Thu Apr 28 13:48:51 2011 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Thu Apr 28 13:48:56 2011 Subject: [Histonet] Supplier for human testis control slides? In-Reply-To: References: Message-ID: I can think of a couple of people I would gladly volunteer. Lynne Bell, HT (ASCP) Histology Team Leader 802-371-4923 From akbitting <@t> geisinger.edu Thu Apr 28 14:30:21 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Apr 28 14:30:27 2011 Subject: [Histonet] Ventana Probes resolution Message-ID: <4DB9880D.2B7F.00C9.1@geisinger.edu> Thanks to all of you who offered your help. I did have a conference call with Ventana this afternoon and all my questions were answered. I'll share with you all what I know so no one else needs to fret needlessly. I didn't receive 2- 4ml vials of EBER Probe. I only received 1 when I ordered it. When you order the 760-1209, you should get 2 vials of Probe 4 ml each. I will be getting the second vial sent out to me tomorrow. So now I will have enough Probe to fill my ISH prep kit. It was a production/shipping error. If Customer Support could have explained that to me this morning, I would never have been so frustrated. Water under the bridge. I have put Ventana back on my Christmas card list. Also, they cannot give you the "recipe" but if your "recipe" doesn't work, a TAS can tell you what to try to fix it. I hope this info is helpful. Angie That's what I know, so thought I'd share. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From sgoebel <@t> mirnarx.com Thu Apr 28 15:16:16 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Apr 28 15:16:20 2011 Subject: [Histonet] Tumors tumors everywhere Message-ID: So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From akemiat3377 <@t> yahoo.com Thu Apr 28 15:25:23 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Apr 28 15:26:09 2011 Subject: [Histonet] Tumors tumors everywhere In-Reply-To: References: Message-ID: <9DD3F5C5-5B04-47DD-A385-AAEC8F1D156D@yahoo.com> Sarah, I am curious; have you tried contacting the BioCare research tech support reps? They are very helpful regarding their products and trouble-shooting any issues that may arise. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Apr 28, 2011, at 1:16 PM, wrote: > So I am staining tumors that were implanted as cells subdermally into > mice. The cells are human. I am trying to do Caspase staining on > these > tumors. The primary is an anti-human rabbit polyclonal, and I am > using > a polymer (Biocare Mach3) in lieu of the secondary antibody. The > background is through the roof!! Could the reason be that the > tumor was > grown in a mouse and is having cross reactivity somehow? What species > antibody should I be using instead? All my mouse monoclonal > antibodies > work perfect on the tissue, it's this stupid rabbit polyclonal!! I am > blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin > (just to see if that would help...it didn't), and protein block (it's > literally an hour worth of blocking!!), developing with DAB (Dako) and > hematoxylin counterstain. I am so confused as how to get this to > work! > Also, it isn't just this particular antibody it is any rabbit > polyclonal > I have tried. Could it be the polymer? It is the one that Biocare > suggested? HELP!! > > Thanks in advance =) > > > > Sarah Goebel, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Thu Apr 28 15:05:51 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Apr 28 15:36:38 2011 Subject: [Histonet] RE: Thanks to all!!!!! In-Reply-To: <59978e12-6cf4-461e-ad00-7c3caada0d46@DCPWPRTR02.mdanderson.edu> References: <59978e12-6cf4-461e-ad00-7c3caada0d46@DCPWPRTR02.mdanderson.edu> Message-ID: Thanks to all!!!!! I am writing this as a thanks to all persons posing questions and responding to them on the digest. I am an Education Coordinator at MD Anderson School of Health Professions and we are implementing a Critical Thinking component to our classes. You would not realize how difficult it is to come up with those type of questions on demand. Brain farts as I call them. The digest will provide me with great ideas to create questions to teach troubleshooting and critical thinking skills to the students. Skills that are sometimes lacking. Some questions just lead me to come up with ones to pose to the students. Staining questions about the H & E are the best. ALWAYS, THE IDENTIFYING INFORMATION WILL BE LEFT OUT TO PROTECT CONFIDENTIALITY.(HIPPA) I appreciate the knowledge and experience that is found here and respect it highly. I have been able to re-connect with long lost co-workers and to pass on any experience that I have to others. Thanks again, Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org **************************************** From alisha <@t> ka-recruiting.com Fri Apr 29 09:08:08 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Fri Apr 29 09:08:23 2011 Subject: [Histonet] Histology Job Opportunity Message-ID: <449786325.1304086088462.JavaMail.cfservice@SL4APP4> Hi Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Histotech position in Western GA (will relocate): I am currently working on an amazing opportunity with an award winning non-profit community hospital in western GA. This well-respected 400 bed hospital is looking to hire on a histotech. They are looking for either an experienced histotech or new graduate who is HT(ASCP) certified. The compensation package is fantastic and includes a competitive hourly rate comprehensive benefits package, tuition reimbursement, and relocation, if needed. If interested in learning more details, please email me at alisha@ka-recruiting.com. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Cytotech and Histotech 1. MI - Histology Manager 2. NY - Rochester - Histotech 3. FL - Jacksonville - Histotech 4. NH - Histology Supervisor 5. NC - Pathology Lab Manager 6. GA - Histology Supervisor 7. ME - Histotech 8. FL - Histotech 9. NY - Histotech 10. NY - Grossing Tech 11. NY - IHC Tech 12. NY - IHC Supervisor 13. NV - Histotech 14. IL - Chicago - Histotech 15. Texas If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From gagnone <@t> KGH.KARI.NET Fri Apr 29 11:03:36 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Fri Apr 29 11:03:47 2011 Subject: [Histonet] C4d stain for IF Message-ID: Patrick, or anyone else using the alpco product...can you give some details/feedback on how this anti human C4d (FITC-Conjugated) is working for you, and whether you were able to replace two steps with one for C4d IF staining? I checked that website, and the pdf on this product reads: "The antibody detects human complement split product C4d and has been tested for use on paraffin sections and frozen sections of human Tissue. " We've switched our goat anti-mouse currently used for C4d immunofluorescence, and would prefer to switch to a conjugated product. It sounds like something we'd like to try. Thanks in advance, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada Try the C4d directly labeled antibody from alpco www.alpco.com anti human C4d (FITC-Conjugated) Cat #: 04-BI-RC4D-FITC Good luck. On Wed, Apr 27, 2011 at 2:54 PM, Butler, Roszetta wrote: > I'm having trouble with my C4d IF stain. I'm using AbDirect/Serotec, mouse monoclonal diluted 1:500 for 1 hr, and the Vector is AbDirect/Serotec diluted 1:25 for ? hour. This is not working and I've tried different dilutions with no success. Can anyone share their protocol? > > Rosie Butler, > AmeriPath/3000 United Founders Blvd./Suite 234/Oklahoma City, OK 73112/405.842.7575 - Office > 405.650.8921 - Cell/405.841.2002 - Fax -- >>Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From Nacaela.Johnson <@t> USONCOLOGY.COM Fri Apr 29 11:06:54 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Fri Apr 29 11:07:57 2011 Subject: [Histonet] Job Opportunities Message-ID: <71882EED22A283429E8424513A22922D0CD17A@txhous1eb015.uson.usoncology.int> Does anyone know of Histology opening in the Kansas City area? I know of an individual who is looking. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From azdudley <@t> hotmail.com Fri Apr 29 11:29:40 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Apr 29 11:29:44 2011 Subject: [Histonet] cap new ANP.22760 Message-ID: hi everyone, just wondering how others were handling this checklist statement? thanks so much for your input. seems like a lot of extra work when running control with each show if each is working. anita dudley providence hosp mobile alabama From settembr <@t> umdnj.edu Fri Apr 29 12:59:35 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Fri Apr 29 12:59:47 2011 Subject: [Histonet] cap new ANP.22760 In-Reply-To: References: Message-ID: ANTIBODY LOTS I have a Dako and I have a Bond too. For the Dako I use my positive control. I use 3 slides. One is treated as the negative control - no antibody One is treated with the current lot One is treated with the new lot. I keep all QC slides together. I keep the 3 slides together. For my Bond, I use 2 slides of my positive control When a new lot comes in I run the new lot and the negative control. Then I compare them to the previous lot that I ran in the past. I keep the slides in chronological order - the previous lot goes back with The slides from that date. The NEW Lot slides get filed with today's date. DETECTION SYSTEM LOTS For the Bond I QC the detection kit using an antibody like Ki-67, it works well and always shows good nuclear staining. I indicate the received date, and QC date. I run the QC on my Ki-67 positive control. I use 2 slides. One is treated as the negative control - no antibody. One is treated with Ki-67. I compare these to the previous Detection kit QC slides I created a written procedures for all of the above. Hope this helps. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Friday, April 29, 2011 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cap new ANP.22760 hi everyone, just wondering how others were handling this checklist statement? thanks so much for your input. seems like a lot of extra work when running control with each show if each is working. anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Fri Apr 29 13:04:18 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Fri Apr 29 13:04:24 2011 Subject: [Histonet] Pax-2 on FFPE human tissue In-Reply-To: References: Message-ID: Hi All, Having a tough time looking for a reliable vendor for Pax-2. What vendors are you using? I need to use this on formalin fixed paraffin embedded human tissue. What control do you use? Thank you. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ From lmdee1 <@t> yahoo.com Fri Apr 29 13:29:23 2011 From: lmdee1 <@t> yahoo.com (Linda) Date: Fri Apr 29 13:29:26 2011 Subject: [Histonet] Could someone please tell me the requirements in work in Germany Message-ID: <384559.74004.qm@web36502.mail.mud.yahoo.com> Hi All, ? I would greatly appreciate if someone could tell me the requirements for working in Germany as a histologist?? Is it hard to get work? ? Thank you in advance, ? Linda Dee, BGS, HT(ASCP) From marktarango <@t> gmail.com Fri Apr 29 13:37:59 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Apr 29 13:38:04 2011 Subject: [Histonet] Pax-2 on FFPE human tissue In-Reply-To: References: Message-ID: Hi Dana, Invitrogen sells a rabbit polyclonal anti-pax-2 antibody that works well on FFPE tissues (Cat # 180483). We use a multi-tissue control block for a control. It has normal kidney to show the endothelial cells around glomeruli and renal tubules staining, clear cell renal cell Ca to show tumor staining and a small piece of tonsil to show that B-cells stain. Hope I helped, Mark On Fri, Apr 29, 2011 at 11:04 AM, Settembre, Dana wrote: > Hi All, > Having a tough time looking for a reliable vendor for Pax-2. > What vendors are you using? > I need to use this on formalin fixed paraffin embedded human tissue. > What control do you use? > Thank you. > > Dana Settembre > Immunohistochemistry Lab > University Hospital - UMDNJ > Newark, NJ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kiran_g <@t> sbcglobal.net Fri Apr 29 16:43:53 2011 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Fri Apr 29 16:43:57 2011 Subject: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. Message-ID: <45567.85076.qm@web180103.mail.gq1.yahoo.com> Hi All, ? What is the standard practice out in histology world?if?hand written cassette id washed?away during processing? ? Please share if you had any experience and how did you resolve this and what is your current practice. ? ? Thank you so much! ? -Kiranjit ? ? ? ? From histotech <@t> imagesbyhopper.com Fri Apr 29 17:04:25 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Apr 29 17:04:37 2011 Subject: [Histonet] Histology Waste in Florida Message-ID: <000301cc06b9$6a470f20$3ed52d60$@imagesbyhopper.com> Hi Histonetters, This is a question specific to Florida. Can you share with me what your facilities do with regards to the disposal of formalin and alcohol? With regards to formalin, do you recycle, haul it away, neutralize etc? With regards to alcohol, do you haul away, pour down the drain, recycle etc? Does anyone have any specific regs that could help answer these questions? Thanks! From histotech <@t> imagesbyhopper.com Fri Apr 29 17:20:23 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Apr 29 17:20:50 2011 Subject: [Histonet] special stain controls Message-ID: <000801cc06bb$a4c819d0$ee584d70$@imagesbyhopper.com> Hi, I am in need of control blocks for special stains, H. Pylori, pneumocystis and AFB. I am attempting to "grow" our own AFB, but am having difficulty getting the other control blocks. Commercial vendors charge a LOT of money for these controls! I was hoping to find a way to swap controls, if I could. Does anyone have any suggestions for me? I don't mind buying them, but tend to cringe when the costs are around $10/slide!! Thanks! From andreahooper <@t> rocketmail.com Fri Apr 29 17:39:10 2011 From: andreahooper <@t> rocketmail.com (andreahooper@rocketmail.com) Date: Fri Apr 29 17:39:25 2011 Subject: [Histonet] Tumors tumors everywhere In-Reply-To: References: Message-ID: <632487033-1304116759-cardhu_decombobulator_blackberry.rim.net-790649411-@bda192.bisx.prod.on.blackberry> Did you check to ensure the secondary in the kit is anti-rabbit IgG, with minimal cross to mouse? Ask Biocare directly. What does the background look like? What does your isotype look like? How about your secondary only control? Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Sender: histonet-bounces@lists.utsouthwestern.edu Date: Thu, 28 Apr 2011 15:16:16 To: Subject: [Histonet] Tumors tumors everywhere So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 41dmb41 <@t> gmail.com Fri Apr 29 18:19:44 2011 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Fri Apr 29 18:19:56 2011 Subject: [Histonet] special stain controls In-Reply-To: <000801cc06bb$a4c819d0$ee584d70$@imagesbyhopper.com> References: <000801cc06bb$a4c819d0$ee584d70$@imagesbyhopper.com> Message-ID: <4EC40ADA-C274-44CA-9E25-2C4FD8DCF84D@gmail.com> I can provide you with all the H. pylori you could ever want if you have some good fungus blocks. Let me know if you'd like to trade! Drew Sent from my iPhone On Apr 29, 2011, at 6:20 PM, wrote: > Hi, > > > > I am in need of control blocks for special stains, H. Pylori, pneumocystis > and AFB. I am attempting to "grow" our own AFB, but am having difficulty > getting the other control blocks. Commercial vendors charge a LOT of money > for these controls! I was hoping to find a way to swap controls, if I > could. Does anyone have any suggestions for me? I don't mind buying them, > but tend to cringe when the costs are around $10/slide!! > > > > Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ddreesen <@t> sbcglobal.net Sat Apr 30 01:17:52 2011 From: ddreesen <@t> sbcglobal.net (Debbie Dreesen) Date: Sat Apr 30 01:18:03 2011 Subject: [Histonet] A really neat site for students In-Reply-To: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net> Message-ID: <986954.21674.qm@web81002.mail.mud.yahoo.com> Hi All, I came across a really neat site that would be helpful for students, or any of us who just want to stay on our toes. The site makes studying?histology fun by using games such as Hangman, Crossword, Matching, etc. I go there now and then and I always come across information that I didn't already have stored in my brain. ? http://www.studystack.com/menu-237664 Debbie Dreesen, HT(ASCP) From gu.lang <@t> gmx.at Sat Apr 30 01:35:06 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Apr 30 01:35:13 2011 Subject: AW: [Histonet] Could someone please tell me the requirements in work inGermany In-Reply-To: <384559.74004.qm@web36502.mail.mud.yahoo.com> References: <384559.74004.qm@web36502.mail.mud.yahoo.com> Message-ID: Hi Linda, I live in Austria, but hope to be able to give some information. In Germany you can find MTLA, who work in histolabs. This is an education, that is done beyond the age of 17, about three years and ends with a diploma. The translation of MTLA would be medical technical lab assistant, but it's not the same as lab assistant in US. Rather like a Biomedical Scientist in UK but without university degree. If your education is on equal level you have the chance to get a job. I recommend to contact the professional association for your questions. info@dvta.de http://www.dvta.de/startseite/dvta-aktuell/ Gudrun -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Linda Gesendet: Freitag, 29. April 2011 20:29 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Could someone please tell me the requirements in work inGermany Hi All,   I would greatly appreciate if someone could tell me the requirements for working in Germany as a histologist?  Is it hard to get work?   Thank you in advance,   Linda Dee, BGS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sherrineely <@t> hotmail.com Sat Apr 30 09:07:12 2011 From: sherrineely <@t> hotmail.com (sherri neely) Date: Sat Apr 30 09:07:16 2011 Subject: [Histonet] Histotechnologist opening in FL Message-ID: There is a Histotechnologist position open at Baptist Health Care Pensacola Fl if any one would like to live near white sand beaches please apply. http://www.ebaptisthealthcare.org/Careers/ Have A Great Day! Sherri Neely From rjbuesa <@t> yahoo.com Sat Apr 30 09:39:14 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 30 09:39:17 2011 Subject: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. In-Reply-To: <45567.85076.qm@web180103.mail.gq1.yahoo.com> Message-ID: <556688.58493.qm@web65715.mail.ac4.yahoo.com> Eleven years ago that happened in our laboratory in what we started to call "The Black Tuesday" (it was from Monday to Tuesday). Thanks that we always kept all cassettes described-written in a log with the type of tissue and the number of pieces with their sizes. Then we went through the list of cassettes that were stored in the baskets in sequential order. That, and the cases description, allowed us to identify all the 268 cassettes. We also informed the chief pathologist and documented it in our QC. The HT that "decided" to use a different pencil to write the cassettes was counseled. We also instituted a check in of the pencil before writing the daily cassette load. I sympathize with your issue, it was really a nightmare in our lab that we were fortunate enough to overcome (thanks to our chain of custody procedure). Ren? J. --- On Fri, 4/29/11, Kiranjit Grewal wrote: From: Kiranjit Grewal Subject: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. To: histonet@lists.utsouthwestern.edu Date: Friday, April 29, 2011, 5:43 PM Hi All, ? What is the standard practice out in histology world?if?hand written cassette id washed?away during processing? ? Please share if you had any experience and how did you resolve this and what is your current practice. ? ? Thank you so much! ? -Kiranjit ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Apr 30 09:41:00 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 30 09:41:04 2011 Subject: [Histonet] Histology Waste in Florida In-Reply-To: <000301cc06b9$6a470f20$3ed52d60$@imagesbyhopper.com> Message-ID: <137942.53747.qm@web65715.mail.ac4.yahoo.com> Formalin ? towed away?by a safety contractor. Alcohol ? down the drain (recycling alcohol is too costly and time consuming). Ren? J. --- On Fri, 4/29/11, histotech@imagesbyhopper.com wrote: From: histotech@imagesbyhopper.com Subject: [Histonet] Histology Waste in Florida To: histonet@lists.utsouthwestern.edu Date: Friday, April 29, 2011, 6:04 PM Hi Histonetters, This is a question specific to Florida.? Can you share with me what your facilities do with regards to the disposal of formalin and alcohol?? With regards to formalin, do you recycle, haul it away, neutralize etc?? With regards to alcohol, do you haul away, pour down the drain, recycle etc? Does anyone have any specific regs that could help answer these questions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abeharry798 <@t> gmail.com Sat Apr 30 10:11:54 2011 From: abeharry798 <@t> gmail.com (Andrea) Date: Sat Apr 30 10:08:06 2011 Subject: [Histonet] Job opportunity- Brampton Ontario Message-ID: <554E6D62-9638-4707-8517-3309ACDF99E6@gmail.com> Hi everyone ,we are looking to fill two PA positions, below is the posting. Thanks, Andrea Beharry WILLIAM OSLER HEALTH SYSTEM JOB OPPORTUNITIES ? NON-UNION POSITION:?Two (2) Full-time Pathologist Assistants ? DEPARTMENT/SITE:?Pathology ? Brampton Civic Hospital? ? HOURS: Days, Occasional weekend hours may be required. ? QUALIFICATIONS:? ?Bachelor of Science in Biology and/or Masters of Pathology (Pathology Practicum University of Manitoba) or equivalent with a minimum of 2 years? experience directly related to duties and responsibilities specified ?Strong knowledge of Anatomy ?Demonstrated skills and ability in examining, describing, dissecting, weighing and photography of small and complex large gross surgical specimens of all tissue types ?Assist with or perform autopsies under pathologists? supervision ?Proven ability to perform duties independently or as a team member, with high degree of motivation and independent judgement ?Familiarity with equipment and tissue processing ?Demonstrated computer proficiency; Meditech and Paradigm an asset ?Proven written and oral communication skills ?Proven excellence working in a team environment with strong interpersonal skills and high degree of professionalism ?Demonstrated strong problem-solving ability ?Demonstrated ability to work under pressure and maintain high level of productivity and competence ?American Association of Pathology Assistants (AAPA) registration an asset ?May be required to work at and travel between all hospital sites ? JOB SUMMARY: Perform a variety of grossing and autopsy duties, and other related duties as assigned.? APPLY TO:?www.williamoslerhc.on.ca? ?? From poupakfar <@t> yahoo.com Sat Apr 30 18:00:07 2011 From: poupakfar <@t> yahoo.com (Poupak Farahani) Date: Sat Apr 30 18:00:17 2011 Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 14 Message-ID: Evimjuytdr e Sent from my iPad On Apr 18, 2011, at 7:43 AM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Renal Catecholamines (Lynne Cates)juuuhggswewfqgb verydffgffhfshopqaDDHWYDCFGHK > 2. RE: Ventana ultra (Kim Tournear) > 3. Re: Ventana ultra (Angela Bitting) > 4. AW: [Histonet] Benchmark Ultra question - follow up (Gudrun Lang) > 5. RE: Ventana ultra (Kuhnla, Melissa) > 6. Re: FW: 1 H&E slide vs. 2 (Rene J Buesa) > 7. RE: Ventana ultra (Amber McKenzie) > 8. RE: Ventana ultra (Setlak, Lisa) > 9. AW: [Histonet] Ventana ultra (Gudrun Lang) > 10. RE: Histonet Digest, Von Kossa Stain (Mayer,Toysha N) > 11. IHC platforms/Sales Representatives (Paula Lucas) > 12. Re: AW: [Histonet] Benchmark Ultra question - follow up > (Angela Bitting) > 13. RE: Ventana ultra (Angela Bitting) > 14. slide brite and coverslip drying time (Dawn Herron) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 13 Apr 2011 10:13:23 -0400 > From: "Lynne Cates" > Subject: [Histonet] Renal Catecholamines > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Does anyone know any places that perform catecholamines on porcine renal > tissue and contact numbers? > > > > Lynne > > > > > > > > ------------------------------ > > Message: 2 > Date: Wed, 13 Apr 2011 07:32:56 -0700 (PDT) > From: Kim Tournear > Subject: RE: [Histonet] Ventana ultra > To: Barbara.Crill@LPNT.net, histonet@lists.utsouthwestern.edu, Sebree > Linda A > Message-ID: <504386.23697.qm@web120220.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I've heard that adding slides and/or reagents can add as much as 8 minutes to the run time. Again, that's just what I've heard, don't know if it's fact or fiction.... > > ~Kim~ > OU ROCKS!!!! > ~Don't be afraid your life will end, > be afraid it will never begin~ > > --- On Wed, 4/13/11, Sebree Linda A wrote: > > > From: Sebree Linda A > Subject: RE: [Histonet] Ventana ultra > To: Barbara.Crill@LPNT.net, histonet@lists.utsouthwestern.edu > Date: Wednesday, April 13, 2011, 2:09 PM > > > Please "Reply All" as I'd like to hear feedback also. > > Thanks. > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Barbara.Crill@LPNT.net > Sent: Wednesday, April 13, 2011 8:39 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ventana ultra > > Does anyone use the Benchmark Ultra - care to share your comments? > > We are considering switching from the Benchmark XT to the Ultra but > would like to hear from users about this instrument. > Is there an increase in reagent cost? > > Can you really add more slides without adding time to the run? > > > ANTOINETTE CRILL, MBA,CT(ASCP) > TEAM LEADER ANATOMIC PATHOLOGY > DANVILLE REGIONAL MEDICAL CENTER > (O) 434.799.4470 > (F) 434.773.6806 > E-mail: barbara.crill@LPNT.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 3 > Date: Wed, 13 Apr 2011 10:41:42 -0400 > From: "Angela Bitting" > Subject: Re: [Histonet] Ventana ultra > To: , > Message-ID: <4DA57DE6.2B7F.00C9.1@geisinger.edu> > Content-Type: text/plain; charset="us-ascii" > > each of the 30 drawers essentially is it's own "run". You don't have to wait for all 30 drawers to finish their runs before adding more slides. > There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. > The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. > > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > >>>> 4/13/2011 9:39 AM >>> > Does anyone use the Benchmark Ultra - care to share your comments? > > We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. > Is there an increase in reagent cost? > > Can you really add more slides without adding time to the run? > > > ANTOINETTE CRILL, MBA,CT(ASCP) > TEAM LEADER ANATOMIC PATHOLOGY > DANVILLE REGIONAL MEDICAL CENTER > (O) 434.799.4470 > (F) 434.773.6806 > E-mail: barbara.crill@LPNT.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > -------------- next part -------------- > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Bitting, Angela > TEL;WORK:570-271-6844 > ORG:;Histology > EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu > N:Bitting;Angela > END:VCARD > > > ------------------------------ > > Message: 4 > Date: Wed, 13 Apr 2011 16:44:27 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Benchmark Ultra question - follow up > To: > Message-ID: <87A14A80DD074AA5A3308A1E3BDD40BA@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > Thank you all for your replies. After all I have to admit, that the cause > was human failure. We found, that the protocols have been changed and noone > did remember. That was after a stopped run, where HIER war clicked off for > the repetition. And afterward only short HIER was clicked on again instead > of mild HIER. ? stupid me! > > > > Bye Gudrun > > > > _____ > > Von: Lynn Lee [mailto:lynnlee2010@live.com] > Gesendet: Mittwoch, 13. April 2011 04:14 > An: gu.lang@gmx.at > Betreff: FW: [Histonet] Benchmark Ultra question - follow up > > > > > One last thought- do you filter the new antibody solution you made EVERY > time when refilling the prep kit? If not, there could be some particulates > in the diluent causing a blockage also. Hope these suggestions help or > maybe you have already checked this out. > > Lynn Lee > > > > > > _____ > > From: lynnlee2010@live.com > To: gu.lang@gmx.at > Subject: RE: [Histonet] Benchmark Ultra question - follow up > Date: Tue, 12 Apr 2011 20:10:43 -0600 > > It's possible the flip lid on the prep kit dispenser top is not completed > closed and seated properly. When the dispense hammer goes down, the pressure > may not be the same, every time if this is the case. I always check the tip > of the dispenser for air bubbles before putting on the machine for EVERY one > whether it is a prefilled Ventana dispenser or my prep kit dispenser. > Finally something could be clogged in a line somewhere. > > I worked in R & D for Ventana for almost 4 years and have since used the XT > and Ultra in other labs and never had a problem with incorrect amount of > reagent being dispensed. I guess there's a first time for anything though. I > would call Ventana Customer Support. They are great! > > Lynn Lee B.S., HT(ASCP) > Tucson, AZ > > >> From: gu.lang@gmx.at >> To: histonet@lists.utsouthwestern.edu >> Date: Tue, 12 Apr 2011 18:23:27 +0200 >> Subject: [Histonet] Benchmark Ultra question - follow up >> >> Today I made a further experiment. I did two titration runs. On one slide > I >> added the working solution by pipette on the other slide I added it by >> pushing the filled PrepKit. >> Both stainings came out beautiful. - There has to be something wrong with >> the dispension-amount. Ventana is informed, I hope they find the culprit. >> >> Bye >> Gudrun >> >> -----Urspr?ngliche Nachricht----- >> Von: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun >> Lang >> Gesendet: Montag, 11. April 2011 18:48 >> An: 'Angela Bitting' >> Cc: histonet@lists.utsouthwestern.edu >> Betreff: AW: [Histonet] Benchmark Ultra question >> >> Yes, that could be true. Some of the PrepKits "go harder" than others. >> Perhaps the pressure of the hammer is not big enough. Sometimes the > nozzles >> are not filled equally. (We press always reagens in the nozzle when we >> prepare the run.) >> >> With the Ventana-System there is an additional dilution, because the >> working-solution is added to a reaction-buffer film under the LCS. Perhaps >> it makes a difference if you dispense the solution with the pipett-tip > under >> the LCS or if the drop falls on the surface of the LCS. That could be a >> matter of the LCS-quality, or buffer-quantity , or . I think I'll become >> mad! >> >> Our pathologists have an uncertain unhappiness with the overall staining >> results, but most of the antibodies work well. Perhaps the majority isn't >> very sensitiv to small changes in the system and the quality-difference >> isn't big enough, but some are easier to "kill". >> >> Regards, Gudrun >> >> >> >> >> >> _____ >> >> Von: Angela Bitting [mailto:akbitting@geisinger.edu] >> Gesendet: Montag, 11. April 2011 18:06 >> An: gu.lang@gmx.at >> Betreff: Re: [Histonet] Benchmark Ultra question >> >> >> >> When I was trained to do titration runs on the BenchmarkXT and Ultras, I > was >> told to titrate 100ul of antibody. I have been suspecting for some time > now >> that the dispensers DON'T dispense a full 100ul. I haven't taken the time > to >> prove it, but your question may have motivated me. >> >> That could explain the weak staining. >> >>>>> "Gudrun Lang" 4/11/2011 11:48 AM >>> >> Hi! >> >> I have some issues with our new Ultra. Since a few days antibodies, that >> work usually well, show up very faint. For example the TTF1 (Novocastra). >> >> I ordered a new bottle, made a titration and found, that the "old" titer >> 1:50 was again well enough. I filled the old PrepKit - and the result was >> very weak. >> >> Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - >> and again the result was very weak. At that time the working-solution was >> only three days old. >> >> >> >> Has anyone an explanation, why the titration works well and the automated >> dispension doesn't? >> >> >> >> Regards >> >> Gudrun Lang >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _____ >> >> IMPORTANT WARNING: The information in this message (and the documents >> attached to it, if any) is confidential and may be legally privileged. It > is >> intended solely for the addressee. Access to this message by anyone else > is >> unauthorized. If you are not the intended recipient, any disclosure, >> copying, distribution or any action taken, or omitted to be taken, in >> reliance on it is prohibited and may be unlawful. If you have received > this >> message in error, please delete all electronic copies of this message (and >> the documents attached to it, if any), destroy any hard copies you may > have >> created and notify me immediately by replying to this email. Thank you. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Wed, 13 Apr 2011 10:45:06 -0400 > From: "Kuhnla, Melissa" > Subject: RE: [Histonet] Ventana ultra > To: , > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I currently have one XT and 3 Ultras. I love the Ultras. There is no > difference in reagent costs. The only thing that I can say remotely > related is that if you keep both platforms, the XT and the ultras have > separate CC1, CC2, and LCS. I am fairly certain the contents of the > bottle are the same, but the label and part numbers are different. > I love the continuous access. I have it set up where the three ultras > are 'top loaded' with set antibodies every day. This eliminates bouncing > things around all the time. Contact me if you would like anymore info. I > would be happy to help. I notice no delay in run times. > melissa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Barbara.Crill@LPNT.net > Sent: Wednesday, April 13, 2011 9:39 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ventana ultra > > Does anyone use the Benchmark Ultra - care to share your comments? > > We are considering switching from the Benchmark XT to the Ultra but > would like to hear from users about this instrument. > Is there an increase in reagent cost? > > Can you really add more slides without adding time to the run? > > > ANTOINETTE CRILL, MBA,CT(ASCP) > TEAM LEADER ANATOMIC PATHOLOGY > DANVILLE REGIONAL MEDICAL CENTER > (O) 434.799.4470 > (F) 434.773.6806 > E-mail: barbara.crill@LPNT.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. > > > > > > ------------------------------ > > Message: 6 > Date: Wed, 13 Apr 2011 07:52:04 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] FW: 1 H&E slide vs. 2 > To: histonet@lists.utsouthwestern.edu, Eugenia Thomas > > Message-ID: <748035.62239.qm@web65706.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > There is not such a study. Cutting 2 vs. 1 H&E/block is merely a preference issue amongst pathologists. Now, if you refer to TWO different levels, that is a different issue. > Two consecutive-serial sections from each block should not have a diagnostic impact BUT two different levels, separated by 20-25 sections, may. > That is a decision to take by the pathologist. > Ren? J. > > --- On Tue, 4/12/11, Eugenia Thomas wrote: > > > From: Eugenia Thomas > Subject: [Histonet] FW: 1 H&E slide vs. 2 > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, April 12, 2011, 11:14 PM > > > > > > > > From: eugenia902d1@hotmail.com > To: histonet@lists.utsouthwestern.edu > Subject: 1 H&E slide vs. 2 > Date: Mon, 11 Apr 2011 13:06:59 -0400 > > > > > Good afternoon everyone, > > Does anyone know of an article or statistics discussing the impact (medical diagnosing) of cutting 2 H&E slides per block verses 1 for all routine work? > > > Genia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 7 > Date: Wed, 13 Apr 2011 09:55:38 -0500 > From: "Amber McKenzie" > Subject: RE: [Histonet] Ventana ultra > To: "Kuhnla, Melissa" , > , > Message-ID: > <03C921A1EAF7F541B16543F6EC6A4B370407E57C@giamail2.Gia.com> > Content-Type: text/plain; charset="us-ascii" > > I recently got an Ultra about a month ago and doing test runs, I have > really enjoyed the continuous feed as well but I have a question...if I > deactivate a product from the Ultra's inventory list I notice it's still > in my XT's inventory list. So, I have to deactivate everything off of > each instrument? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, > Melissa > Sent: Wednesday, April 13, 2011 9:45 AM > To: Barbara.Crill@LPNT.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Ventana ultra > > I currently have one XT and 3 Ultras. I love the Ultras. There is no > difference in reagent costs. The only thing that I can say remotely > related is that if you keep both platforms, the XT and the ultras have > separate CC1, CC2, and LCS. I am fairly certain the contents of the > bottle are the same, but the label and part numbers are different. > I love the continuous access. I have it set up where the three ultras > are 'top loaded' with set antibodies every day. This eliminates bouncing > things around all the time. Contact me if you would like anymore info. I > would be happy to help. I notice no delay in run times. > melissa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Barbara.Crill@LPNT.net > Sent: Wednesday, April 13, 2011 9:39 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ventana ultra > > Does anyone use the Benchmark Ultra - care to share your comments? > > We are considering switching from the Benchmark XT to the Ultra but > would like to hear from users about this instrument. > Is there an increase in reagent cost? > > Can you really add more slides without adding time to the run? > > > ANTOINETTE CRILL, MBA,CT(ASCP) > TEAM LEADER ANATOMIC PATHOLOGY > DANVILLE REGIONAL MEDICAL CENTER > (O) 434.799.4470 > (F) 434.773.6806 > E-mail: barbara.crill@LPNT.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail, and any attachments therein, is > confidential and for use by the intended addressee only. If this message > is received by you in error please do not disseminate or read further. > Please reply to the sender that you have received the message in error, > then delete the message. Although Catholic Health Services of Long > Island attempts to sweep e-mail and attachments for viruses, it does > not guarantee that either are virus-free and accepts no liability for > any damage sustained as a result of viruses. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Wed, 13 Apr 2011 09:54:44 -0500 > From: "Setlak, Lisa" > Subject: RE: [Histonet] Ventana ultra > To: 'Angela Bitting' , > "histonet@lists.utsouthwestern.edu" > , "Barbara.Crill@LPNT.net" > > Message-ID: > <7111DB39D045004C9CF29E79C71B28BC101D507915@CMHEXCC01MBX.childrensmemorial.org> > > Content-Type: text/plain; charset="us-ascii" > > We like ours as well. It's not as continual as we originally thought.... to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a "landing zone" in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run). > Lisa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting > Sent: Wednesday, April 13, 2011 9:42 AM > To: histonet@lists.utsouthwestern.edu; Barbara.Crill@LPNT.net > Subject: Re: [Histonet] Ventana ultra > > each of the 30 drawers essentially is it's own "run". You don't have to wait for all 30 drawers to finish their runs before adding more slides. > There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. > The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. > > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > >>>> 4/13/2011 9:39 AM >>> > Does anyone use the Benchmark Ultra - care to share your comments? > > We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. > Is there an increase in reagent cost? > > Can you really add more slides without adding time to the run? > > > ANTOINETTE CRILL, MBA,CT(ASCP) > TEAM LEADER ANATOMIC PATHOLOGY > DANVILLE REGIONAL MEDICAL CENTER > (O) 434.799.4470 > (F) 434.773.6806 > E-mail: barbara.crill@LPNT.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > > > ------------------------------ > > Message: 9 > Date: Wed, 13 Apr 2011 17:01:35 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] Ventana ultra > To: > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <49E6A03ED68944AEA42E8F4F115F9012@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > What we like about the Ultra is, that there's no need for cleaning runs > between the runs and that you can take out the bulk and waste containers > during the run. > We don't use the continious loading, because it doesn't fit to our workplan > and because we use various antibodies from run to run. > The runs are faster, but more than 2 runs per 8 hour shift and 1 run over > night are not possible for us. That's the same workload we did with the XT. > > There are "windows" during the run, when you can add new slides and reagens. > If the needed reagens in on the instrument, you can add them any time, if > there's enough place. But the run will last longer and you have to wait for > a "window" of the longer run to get the reagens off the instrument. Ready > stained slides can be taken out independently. But the free pads cannot be > filled and runs started, until you have the possibility to add the right > reagens. So we decided to stay with "patch-workload" and real go-away. > > Bye > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > Barbara.Crill@LPNT.net > Gesendet: Mittwoch, 13. April 2011 15:39 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Ventana ultra > > Does anyone use the Benchmark Ultra - care to share your comments? > > We are considering switching from the Benchmark XT to the Ultra but would > like to hear from users about this instrument. > Is there an increase in reagent cost? > > Can you really add more slides without adding time to the run? > > > ANTOINETTE CRILL, MBA,CT(ASCP) > TEAM LEADER ANATOMIC PATHOLOGY > DANVILLE REGIONAL MEDICAL CENTER > (O) 434.799.4470 > (F) 434.773.6806 > E-mail: barbara.crill@LPNT.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Wed, 13 Apr 2011 10:12:18 -0500 > From: "Mayer,Toysha N" > Subject: [Histonet] RE: Histonet Digest, Von Kossa Stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > We used the lamp at our cutting stations, coplin jar in our waterbath or on top of the embedder, wrapped in tin foil for 1 hr. > > Toysha N. Mayer, MBA, HT (ASCP) > Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > > > > > > > > > > ------------------------------ > > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 13 Apr 2011 08:29:39 -0700 > From: "Paula Lucas" > Subject: [Histonet] IHC platforms/Sales Representatives > To: > Message-ID: <6619BD7E2C004B4FA2E53529F39EB9B1@biopath.local> > Content-Type: text/plain; charset="us-ascii" > > Hello, > > > > We are inquiring about IHC staining platforms and we've already contacted > the Leica sales rep for the Bond. I would also like to contact Ventana to > get a quote on their system, and I've tried to get in contact with a sales > rep in our area but I haven't had anyone returning my calls. If there is a > Vantana rep reading this, would you please contact the sales representative > for my area and let him/her know we are interested in a quote? We are > located in Orange County (Fountain Valley), California. > > > > For those who have experience with these two platforms, would you mind > taking the time to share with me your views and experiences about the two > systems? > > > > Thanks so much, > > Paula Lucas > > Lab Manager > > Bio-Path Medical Group > > Fountain Valley, CA > > 714.433.1330 > > > > ------------------------------ > > Message: 12 > Date: Wed, 13 Apr 2011 11:30:43 -0400 > From: "Angela Bitting" > Subject: Re: AW: [Histonet] Benchmark Ultra question - follow up > To: , > Message-ID: <4DA58963.2B7F.00C9.1@geisinger.edu> > Content-Type: text/plain; charset=UTF-8 > > Augh! I hate when that happens! > >>>> "Gudrun Lang" 4/13/2011 10:44 AM >>> > Thank you all for your replies. After all I have to admit, that the > cause > was human failure. We found, that the protocols have been changed and > noone > did remember. That was after a stopped run, where HIER war clicked off > for > the repetition. And afterward only short HIER was clicked on again > instead > of mild HIER. ??? stupid me! > > > > Bye Gudrun > > > > _____ > > Von: Lynn Lee [mailto:lynnlee2010@live.com] > Gesendet: Mittwoch, 13. April 2011 04:14 > An: gu.lang@gmx.at > Betreff: FW: [Histonet] Benchmark Ultra question - follow up > > > > > One last thought- do you filter the new antibody solution you made > EVERY > time when refilling the prep kit? If not, there could be some > particulates > in the diluent causing a blockage also. Hope these suggestions help > or > maybe you have already checked this out. > > Lynn Lee > > > > > > _____ > > From: lynnlee2010@live.com > To: gu.lang@gmx.at > Subject: RE: [Histonet] Benchmark Ultra question - follow up > Date: Tue, 12 Apr 2011 20:10:43 -0600 > > It's possible the flip lid on the prep kit dispenser top is not > completed > closed and seated properly. When the dispense hammer goes down, the > pressure > may not be the same, every time if this is the case. I always check > the tip > of the dispenser for air bubbles before putting on the machine for > EVERY one > whether it is a prefilled Ventana dispenser or my prep kit dispenser. > Finally something could be clogged in a line somewhere. > > I worked in R & D for Ventana for almost 4 years and have since used > the XT > and Ultra in other labs and never had a problem with incorrect amount > of > reagent being dispensed. I guess there's a first time for anything > though. I > would call Ventana Customer Support. They are great! > > Lynn Lee B.S., HT(ASCP) > Tucson, AZ > > >> From: gu.lang@gmx.at >> To: histonet@lists.utsouthwestern.edu >> Date: Tue, 12 Apr 2011 18:23:27 +0200 >> Subject: [Histonet] Benchmark Ultra question - follow up >> >> Today I made a further experiment. I did two titration runs. On one > slide > I >> added the working solution by pipette on the other slide I added it > by >> pushing the filled PrepKit. >> Both stainings came out beautiful. - There has to be something wrong > with >> the dispension-amount. Ventana is informed, I hope they find the > culprit. >> >> Bye >> Gudrun >> >> -----Urspr??ngliche Nachricht----- >> Von: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > Gudrun >> Lang >> Gesendet: Montag, 11. April 2011 18:48 >> An: 'Angela Bitting' >> Cc: histonet@lists.utsouthwestern.edu >> Betreff: AW: [Histonet] Benchmark Ultra question >> >> Yes, that could be true. Some of the PrepKits "go harder" than > others. >> Perhaps the pressure of the hammer is not big enough. Sometimes the > nozzles >> are not filled equally. (We press always reagens in the nozzle when > we >> prepare the run.) >> >> With the Ventana-System there is an additional dilution, because the >> working-solution is added to a reaction-buffer film under the LCS. > Perhaps >> it makes a difference if you dispense the solution with the > pipett-tip > under >> the LCS or if the drop falls on the surface of the LCS. That could be > a >> matter of the LCS-quality, or buffer-quantity , or . I think I'll > become >> mad! >> >> Our pathologists have an uncertain unhappiness with the overall > staining >> results, but most of the antibodies work well. Perhaps the majority > isn't >> very sensitiv to small changes in the system and the > quality-difference >> isn't big enough, but some are easier to "kill". >> >> Regards, Gudrun >> >> >> >> >> >> _____ >> >> Von: Angela Bitting [mailto:akbitting@geisinger.edu] >> Gesendet: Montag, 11. April 2011 18:06 >> An: gu.lang@gmx.at >> Betreff: Re: [Histonet] Benchmark Ultra question >> >> >> >> When I was trained to do titration runs on the BenchmarkXT and > Ultras, I > was >> told to titrate 100ul of antibody. I have been suspecting for some > time > now >> that the dispensers DON'T dispense a full 100ul. I haven't taken the > time > to >> prove it, but your question may have motivated me. >> >> That could explain the weak staining. >> >>>>> "Gudrun Lang" 4/11/2011 11:48 AM >>> >> Hi! >> >> I have some issues with our new Ultra. Since a few days antibodies, > that >> work usually well, show up very faint. For example the TTF1 > (Novocastra). >> >> I ordered a new bottle, made a titration and found, that the "old" > titer >> 1:50 was again well enough. I filled the old PrepKit - and the result > was >> very weak. >> >> Then I thought, the PrepKit itself is the culprit. I changed the > PrepKit - >> and again the result was very weak. At that time the working-solution > was >> only three days old. >> >> >> >> Has anyone an explanation, why the titration works well and the > automated >> dispension doesn't? >> >> >> >> Regards >> >> Gudrun Lang >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _____ >> >> IMPORTANT WARNING: The information in this message (and the > documents >> attached to it, if any) is confidential and may be legally > privileged. It > is >> intended solely for the addressee. Access to this message by anyone > else > is >> unauthorized. If you are not the intended recipient, any disclosure, >> copying, distribution or any action taken, or omitted to be taken, > in >> reliance on it is prohibited and may be unlawful. If you have > received > this >> message in error, please delete all electronic copies of this message > (and >> the documents attached to it, if any), destroy any hard copies you > may > have >> created and notify me immediately by replying to this email. Thank > you. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 13 > Date: Wed, 13 Apr 2011 11:36:20 -0400 > From: "Angela Bitting" > Subject: RE: [Histonet] Ventana ultra > To: "Lisa Setlak" , > "histonet@lists.utsouthwestern.edu" > , "Barbara.Crill@LPNT.net" > > Message-ID: <4DA58AB4.2B7F.00C9.1@geisinger.edu> > Content-Type: text/plain; charset=US-ASCII > > We load our Ultras with the most commonly used antibodies each morning. So that minimizes the number of landing zones we need to use. > Also, (if you run both XTs and Ultras) run your antibodies with the longest protocols on Ultra and that will shorten your XT runs. > >>>> "Setlak, Lisa" 4/13/2011 10:54 AM >>> > We like ours as well. It's not as continual as we originally thought.... to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a "landing zone" in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run). > Lisa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting > Sent: Wednesday, April 13, 2011 9:42 AM > To: histonet@lists.utsouthwestern.edu; Barbara.Crill@LPNT.net > Subject: Re: [Histonet] Ventana ultra > > each of the 30 drawers essentially is it's own "run". You don't have to wait for all 30 drawers to finish their runs before adding more slides. > There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. > The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. > > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > >>>> 4/13/2011 9:39 AM >>> > Does anyone use the Benchmark Ultra - care to share your comments? > > We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. > Is there an increase in reagent cost? > > Can you really add more slides without adding time to the run? > > > ANTOINETTE CRILL, MBA,CT(ASCP) > TEAM LEADER ANATOMIC PATHOLOGY > DANVILLE REGIONAL MEDICAL CENTER > (O) 434.799.4470 > (F) 434.773.6806 > E-mail: barbara.crill@LPNT.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > > > > ------------------------------------------------------------------------- > This message was secured by ZixCorp(R). > > > ------------------------------ > > Message: 14 > Date: Wed, 13 Apr 2011 11:16:48 -0500 > From: Dawn Herron > Subject: [Histonet] slide brite and coverslip drying time > To: Histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=ISO-8859-1 > > Hello all. We have just made the transition from xylene in our linear > stainer to Slide Brite. We are also coverslipping from Slide Brite with > Permount. So far the slides look good (though we have to be careful about > water contamination in the dehydrating alcohols) but we are having a problem > with extremely long coverslipping drying time. Some of the slides have been > on the warmer for over 18 hours and you can still move the coverslips! > (Normally the slides would dry within 4 hours on the warmer with xylene and > acrymount) Any suggestions on how to speed drying time? > > Thanks, > Dawn > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 89, Issue 14 > **************************************** From kiran_g <@t> sbcglobal.net Sat Apr 30 19:12:40 2011 From: kiran_g <@t> sbcglobal.net (kiran_g@sbcglobal.net) Date: Sat Apr 30 19:12:46 2011 Subject: [Histonet]Need input: Ink dissolved from Cassettes during processing. In-Reply-To: <556688.58493.qm@web65715.mail.ac4.yahoo.com> References: <45567.85076.qm@web180103.mail.gq1.yahoo.com><556688.58493.qm@web65715.mail.ac4.yahoo.com> Message-ID: <1025397262-1304208760-cardhu_decombobulator_blackberry.rim.net-906243174-@bda2889.bisx.prod.on.blackberry> Thank you! Does anybody take a digital photo to correlate cassettes when using hand written cassettes as a back up? Or Do you put a piece of paper inside the cassette as back up for hand written cassettes? Need input so we can prevent future incidents. Thx Kiranjit Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Sat, 30 Apr 2011 07:39:14 To: ; Kiranjit Grewal Subject: Re: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. Eleven years ago that happened in our laboratory in what we started to call "The Black Tuesday" (it was from Monday to Tuesday). Thanks that we always kept all cassettes described-written in a log with the type of tissue and the number of pieces with their sizes. Then we went through the list of cassettes that were stored in the baskets in sequential order. That, and the cases description, allowed us to identify all the 268 cassettes. We also informed the chief pathologist and documented it in our QC. The HT that "decided" to use a different pencil to write the cassettes was counseled. We also instituted a check in of the pencil before writing the daily cassette load. I sympathize with your issue, it was really a nightmare in our lab that we were fortunate enough to overcome (thanks to our chain of custody procedure). Ren? J. --- On Fri, 4/29/11, Kiranjit Grewal wrote: From: Kiranjit Grewal Subject: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. To: histonet@lists.utsouthwestern.edu Date: Friday, April 29, 2011, 5:43 PM Hi All, ? What is the standard practice out in histology world?if?hand written cassette id washed?away during processing? ? Please share if you had any experience and how did you resolve this and what is your current practice. ? ? Thank you so much! ? -Kiranjit ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet