[Histonet] Vectastain elite ABC kit- multiple dips ok?

[Teresa Iglesias]

Hi all,
I have three 24-well plates with three brain sections in each well. For IHC,
do I have to prepare three different/fresh ABC dips for each 24-well screen
plate or can I incubate (30min) each successive screen plate into the
already used dip of ABC? Do I have to leave it in longer to allow adequate
binding?
I know you can do this with DAB but you have a visual indicator that you've
left it in long enough in that case.
Any experience with this?
Thanks,
-Teresa

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Teresa Iglesias
Graduate Group in Animal Behavior
Department of Evolution and Ecology
University of California-Davis
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