[Histonet] Steedman polyester wax

[Onstead-Cardinale, Luisa]

I would like to know if anyone has a good protocol that includes tissue
fixation (preferred fixative,  fixation temperature etc) and  tissue
processing (time etc.) for   low melting Steedman polyester wax . The
tissue to be embedded is chick embryo brain.

 I am going to do all the tissue processing and embedding  manually. Any
suggestion?

Looking forward to receive your advice

Luisa

 

 

Luisa Onstead-Cardinale, M.S.

Research Associate

UF Biomedical   Research Laboratory, Room 121

655 West 11th Street 

Jacksonville, Fl 32206

phone (904) 633-0984