[Histonet] filtering cytology stains

Feher, Stephen sfeher <@t> CMC-NH.ORG
Thu Sep 16 09:58:55 CDT 2010


Hi Brandi,

Depending on your stain line set up and the number of FNA's that you are
doing, you may want to consider having multiple stain set up's that you
can switch to in a hurry.  This works well if you only filter stains and
change alcohols.  You can keep several boats or the smaller manual
staining dishes filled with Hematoxlyn, OG and EA.  Even better and
quicker if you use the EA/OG combo stain.

The stain dishes or boats that were used for positive FNA's can be
tagged with a colored sticker and set aside to be filtered all together.



Steve

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of louise
renton
Sent: Thursday, September 16, 2010 3:16 AM
To: Brandi Higgins; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] filtering cytology stains

Hi - why not look at something like a millipore (millivex) syringe
filter?
This website has the application choice on it - or perhaps speak to your
Millipore rep?

http://www.millipore.com/techpublications/tech1/pb1951en00


On Wed, Sep 15, 2010 at 6:49 PM, Brandi Higgins
<brandihiggins <@t> gmail.com>wrote:

> Hello All,
>
>     I was wondering how you are filtering your cytology stains.  We 
> are a relatively small lab, so we have been gravity filtering our 
> cytology stains, but as we are getting busier with a larger volume of 
> slides, especially fna's this is becoming a very time consuming 
> process.  I think vacuum filtration would be almost as lengthy a 
> process.
>     It was suggested that we look into getting some 200ml syringes 
> that can come with an attached filter to suck in and pump out the 
> fluid for faster filtration...is anyone using such a process?  If so, 
> do you have product names/numbers for the filters or the syringes.  If

> anyone has another method they are using I would like to hear any 
> suggestions.
>
> Thanks in advance for you input,
> Brandi Higgins, BS, HT(ASCP)
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--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
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073 5574456 (emergencies only)
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