[Histonet] Re: Problem with liver fixation

[Itai Moshe]

Hi Histonet's
I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with
PFA.
I'm using this protocol:
http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997
with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079)
The staining is beautiful, but i can't see the nuclei good enough.
1) Is there a way to enhance the nuclei staining ? (the nuclei is the only
reason that i"m not using the simpler sirius red and fast green staining.)
2) What is the meaning for washing in running tap water washing, is it done
by putting the slides in a  jar with simple tap water for a few minutes ?

Thank's
Itai