[Histonet] Napsin A

[Mark Tarango]

Hi Jessica,

Our control for this antibody is normal lung, adenocarcinoma of the lung,
and thyroid.  We use this same block for other stains too (TTF-1,
sufactant-A, EMA, etc.) but if I were going to make a control block specific
for this antibody, I would want to include a squamous cell carcinoma of the
lung to show that it's negative for napsin-A.  You may see a very tiny
amount of staining on the squamous cell carcinomas where the
pulmonary-aveolar macrophages are eating up the keratin, but the tumor cells
will be negative.

Ovarian carcinomas can also stain.  I would include a few of those too so
the docs can see what that looks like.
I would also make sure to include thyroid and thyroid carcinomas.  With a
particular vendor's antibody, napsin-a seems to stain thyroid (I don't want
to name names), but with Novocastra's antibody it doesn't.  It was helpful
to our pathologists to have an antibody that doesn't stain thyroid.

Thanks
Mark

On Mon, Sep 13, 2010 at 8:57 AM, Jessica Piche <jhisto37 <@t> yahoo.com> wrote:

> Hello Everyone,
>
> We are in the process of working up Napsin A. I was wondering what tissue
> others are using for multi tissue blocks to validate the anitbody? Thanks,
>
>
> Jessica Piche-Grocki, HT(ASCP)
> Waterbury Hospital, CT
>
>
>
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