[Histonet] H&E+ Alcian Blue
Deborah Faichney
d.a.faichney <@t> stir.ac.uk
Thu Sep 9 03:44:39 CDT 2010
Hello all,
I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it?
For information, I am trying to stain salmon intestine at 5 microns for using the following:
Alcian Blue 8GX (certified), pH has been checked
Haematoxylin Z (Cellpath Uk)
1% aq Eosin (Cellpath uk) and lab prepared solution from dye.
Thanks from a frustrated technician!
Debbie Faichney
Histopathology
Institute of Aquaculture
University of Stirling
Scotland
UK
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