From chak_bou <@t> yahoo.com Wed Sep 1 05:39:13 2010 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Wed Sep 1 05:39:17 2010 Subject: [Histonet] Thank you! Message-ID: <919256.47439.qm@web58101.mail.re3.yahoo.com> Hi Histonet, I just would like to thank everyone who responded to my question about Warthin-Starry stain. Your help is very appreciated! Chakib From amario3 <@t> uic.edu Wed Sep 1 08:45:50 2010 From: amario3 <@t> uic.edu (Andrea Marion) Date: Wed Sep 1 08:45:57 2010 Subject: [Histonet] melted paraffin storage and dispensing Message-ID: I do histology in a small research lab. We use the tissue processor and embedding station of a nearby lab to process specimens. I would like to switch to manual in-lab processing of some our more delicate samples like mouse embryos. I am wondering what histonetters use to store their melted paraffin when you don't have access to an embedding station. I have an appropriate incubator, but what kind of container should be used? I have seen metal paraffin pails used elsewhere - anyone know where to purchase these? Is a metal container necessary or will a plastic or glass beaker work? Are there any problems with storing paraffin (Paraplast Plus) between uses in the liquid state, or should I remelt it each time I want to embed samples? Anything else I should be concerned about that I am overlooking? Thanks, Andrea Marion Graduate Student University of Illinois at Chicago amario3 |at| uic |dot| edu From brandihiggins <@t> gmail.com Wed Sep 1 12:30:51 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Wed Sep 1 12:30:55 2010 Subject: [Histonet] tonsils In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A558E@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A558E@MAIL1.pph.local> Message-ID: > > We receive and process all tonsils. We charge 88304. > Brandi Higgins, BS, HT (ASCP) From camayton <@t> gmail.com Wed Sep 1 13:10:34 2010 From: camayton <@t> gmail.com (Cathy Mayton) Date: Wed Sep 1 13:10:40 2010 Subject: [Histonet] Attention new equipment distributors Message-ID: Can you give me a ball park figure for a new paraffin embedding center? I have a friend setting up a new lab and are working on their budget. They need a cost of a unit equivalent to the Shandon Histocentre that has the electric forceps. About 7 years ago I paid about $7000 for a unit such as this but of course time and costs have changed things. Please respond off line to camayton@gmail.com Thanks in advance, -- Cathy Mayton From brandihiggins <@t> gmail.com Wed Sep 1 13:12:02 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Wed Sep 1 13:12:07 2010 Subject: [Histonet] tonsils In-Reply-To: <4C7E5E4E.7400.0077.1@harthosp.org> References: <2820431BF953BB4DA3E9E1A5882265FD034A558E@MAIL1.pph.local> <4C7E5E4E.7400.0077.1@harthosp.org> Message-ID: correct. Pathologists do microscopic examination and generate a report with microscopic diagnosis for all tonsils. Brandi Higgins, BS, HT(ASCP) On Wed, Sep 1, 2010 at 2:08 PM, Richard Cartun wrote: > It sounds like you do microscopic examination on all tonsils? > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> Brandi Higgins 9/1/2010 1:30 PM >>> > > > > We receive and process all tonsils. We charge 88304. > > > > Brandi Higgins, BS, HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From MSHERWOOD <@t> PARTNERS.ORG Wed Sep 1 13:26:41 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Sep 1 13:28:44 2010 Subject: [Histonet] accu-edge low profile microtome blades References: <57BE698966D5C54EAE8612E8941D7683097225DF@EXCHANGE3.huntingtonhospital.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708AF0027@PHSXMB30.partners.org> We use the low-profile microtome blades and love them. We actually found Thermo-Fisher's brand of the low-profile to work just as well and they are less expensive. We had problems with other manufacturers' low -profile blades that had the oil on them. However we have several sample boxes and I will have the techs wipe the oil off and see if they work better. Thanks, Laurie. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Mon 8/30/2010 4:25 PM To: Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We also use those blades and love them. We've tried others but always come back to the Accu-edge. I have always noticed a film of oil on the blades. I usually wipe the edge of the blade with a kimwipe before using it. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Monday, August 30, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] accu-edge low profile microtome blades Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From billodonnell <@t> catholichealth.net Wed Sep 1 13:54:20 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Sep 1 13:54:34 2010 Subject: [Histonet] RE: accu-edge low profile microtome blades In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708AF0027@PHSXMB30.partners.org> References: <57BE698966D5C54EAE8612E8941D7683097225DF@EXCHANGE3.huntingtonhospital.com> <073AE2BEA1C2BA4A8837AB6C4B943D9708AF0027@PHSXMB30.partners.org> Message-ID: The oil serves a valuable purpose and shopuld be found on all blades of high quality. It protects against oxidation and allows the blades to be easily separated from one another, so the oil has always been there. I have personally found no advantage to wiping or not wiping the oil from the slides. My 2cents - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, September 01, 2010 1:27 PM To: Laurie Colbert; Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We use the low-profile microtome blades and love them. We actually found Thermo-Fisher's brand of the low-profile to work just as well and they are less expensive. We had problems with other manufacturers' low -profile blades that had the oil on them. However we have several sample boxes and I will have the techs wipe the oil off and see if they work better. Thanks, Laurie. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Mon 8/30/2010 4:25 PM To: Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We also use those blades and love them. We've tried others but always come back to the Accu-edge. I have always noticed a film of oil on the blades. I usually wipe the edge of the blade with a kimwipe before using it. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Monday, August 30, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] accu-edge low profile microtome blades Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmconway <@t> usgs.gov Wed Sep 1 14:18:15 2010 From: cmconway <@t> usgs.gov (Carla M Conway) Date: Wed Sep 1 14:18:24 2010 Subject: [Histonet] Frozen sections missing centers Message-ID: Hello, I have been asked to to prepare frozen sections of fish livers that have been stored in RNAlater Solution at -80C. Tissues (about 10 um square x 5 um thick) were embedded in OCT, frozen on dry ice, and sectioned at 6 um. My problem is that when I cut a section, the center of the tissue is missing! Approximately 1 mm of tissue around the edges seems to section just fine, but then there is just a gaping hole in the center as if the tissue is receding while it is being cut. I've tried sectioning at various temperatures (-20C, -16C, and -12C) and thickness (4um-8um). Any help will be greatly appreciated, Carla Conway Western Fisheries Research Center Seattle, WA ph: 206-526-6282 ext. 242 cmconway@usgs.gov From TGoins <@t> mt.gov Wed Sep 1 14:23:41 2010 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Sep 1 14:23:49 2010 Subject: [Histonet] Microtome Blades Message-ID: The best blades we've used so far are from Mercedes Medical - MER DMBHPGP - and are priced well below some competitors blades. Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana From Sandra.Harrison3 <@t> va.gov Wed Sep 1 14:49:38 2010 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Wed Sep 1 14:49:42 2010 Subject: [Histonet] accu-edge low profile microtome blades In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708AF0027@PHSXMB30.partners.org> References: <57BE698966D5C54EAE8612E8941D7683097225DF@EXCHANGE3.huntingtonhospital.com> <073AE2BEA1C2BA4A8837AB6C4B943D9708AF0027@PHSXMB30.partners.org> Message-ID: About 1/2 of my tech.'s wipe the oil off; otherwise it causes the first 2-3 ribbons to curl off the knife, rather than forming a nice ribbon. Sandy Harrison Histology Supervisor Minneapolis VA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, September 01, 2010 1:27 PM To: Laurie Colbert; Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We use the low-profile microtome blades and love them. We actually found Thermo-Fisher's brand of the low-profile to work just as well and they are less expensive. We had problems with other manufacturers' low -profile blades that had the oil on them. However we have several sample boxes and I will have the techs wipe the oil off and see if they work better. Thanks, Laurie. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Mon 8/30/2010 4:25 PM To: Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We also use those blades and love them. We've tried others but always come back to the Accu-edge. I have always noticed a film of oil on the blades. I usually wipe the edge of the blade with a kimwipe before using it. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Monday, August 30, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] accu-edge low profile microtome blades Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Cortright <@t> vtmednet.org Thu Sep 2 07:25:29 2010 From: Valerie.Cortright <@t> vtmednet.org (Cortright, Valerie) Date: Thu Sep 2 07:25:39 2010 Subject: [Histonet] Xylene/Solvent Waste Message-ID: <6C6628BE75C6374F96168DB50F9CE30E015AEB51B00C@EMAIL2.fahc.fletcherallen.org> Hello! I was wondering if anyone has any data on the amount of xylene/solvent waste that they create on a monthly basis? I am looking for gallons or lbs of waste created! Any help you can provide would be greatly appreciated... feel free to email me directly. Thanks so much, Valerie Cortright From rjbuesa <@t> yahoo.com Thu Sep 2 08:33:09 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 2 08:33:13 2010 Subject: [Histonet] Xylene/Solvent Waste In-Reply-To: <6C6628BE75C6374F96168DB50F9CE30E015AEB51B00C@EMAIL2.fahc.fletcherallen.org> Message-ID: <463022.51993.qm@web65703.mail.ac4.yahoo.com> That will depend on the original usage determined by the work volume. You will have to make your determination yourself for your particular conditions and the data from others will be only useful for comparative purposes, i.e. are you generating more or less waste than others. Ren? J. --- On Thu, 9/2/10, Cortright, Valerie wrote: From: Cortright, Valerie Subject: [Histonet] Xylene/Solvent Waste To: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, September 2, 2010, 8:25 AM Hello! ? I was wondering if anyone has any data on the amount of xylene/solvent waste that they create on a monthly basis?? I am looking for gallons or lbs of waste created! Any help you can provide would be greatly appreciated... feel free to email me directly. Thanks so much, Valerie Cortright _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Cortright <@t> vtmednet.org Thu Sep 2 08:44:18 2010 From: Valerie.Cortright <@t> vtmednet.org (Cortright, Valerie) Date: Thu Sep 2 08:44:22 2010 Subject: [Histonet] Xylene/Solvent Waste In-Reply-To: <463022.51993.qm@web65703.mail.ac4.yahoo.com> References: <6C6628BE75C6374F96168DB50F9CE30E015AEB51B00C@EMAIL2.fahc.fletcherallen.org> <463022.51993.qm@web65703.mail.ac4.yahoo.com> Message-ID: <6C6628BE75C6374F96168DB50F9CE30E015AEB51B00F@EMAIL2.fahc.fletcherallen.org> I am using it for comparative purposes, so any data will be useful to me. I have my own numbers, I'm just looking to see how we compare to other institutions. Thanks ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 02, 2010 9:33 AM To: 'histonet@lists.utsouthwestern.edu'; Cortright, Valerie Subject: Re: [Histonet] Xylene/Solvent Waste That will depend on the original usage determined by the work volume. You will have to make your determination yourself for your particular conditions and the data from others will be only useful for comparative purposes, i.e. are you generating more or less waste than others. Ren? J. --- On Thu, 9/2/10, Cortright, Valerie wrote: From: Cortright, Valerie Subject: [Histonet] Xylene/Solvent Waste To: "'histonet@lists.utsouthwestern.edu'" Date: Thursday, September 2, 2010, 8:25 AM Hello! I was wondering if anyone has any data on the amount of xylene/solvent waste that they create on a monthly basis? I am looking for gallons or lbs of waste created! Any help you can provide would be greatly appreciated... feel free to email me directly. Thanks so much, Valerie Cortright _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Thu Sep 2 09:29:47 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Thu Sep 2 09:30:41 2010 Subject: [Histonet] Hazardous waste Message-ID: Histonetters We are just now keeping a count of how many gallons we dispose of because the company that hauls it off gave us a 55 gal drum and charges us for 55 gal drum everytime he picks it up so we are going for a count for one month to see how much we dispose uf and maybe get a smaller drum so not to have to pay as much. Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From MadaryJ <@t> MedImmune.com Thu Sep 2 12:31:35 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Thu Sep 2 12:31:40 2010 Subject: [Histonet] embedding center just did alot of research! Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A13024DD901@MD1EV002.medimmune.com> I did a lot of research on embedding centers and made the mistake of going with the least expensive new one. If you want to know what I bought email me separately. The prices are in the stratosphere but I have to say Leica still has the edge for this 30 plus year tech. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From malbenatti <@t> gmail.com Thu Sep 2 12:33:13 2010 From: malbenatti <@t> gmail.com (Malika Benatti) Date: Thu Sep 2 12:33:27 2010 Subject: [Histonet] H1B Visa Sponsorship Message-ID: I wonder if anyone can help or perhaps knows anyone who could. I am a UK trained and registered medical technologist and have specialised in Histology, with 16 years laboratory experience. I am fully registered as a Specialist Biomedical Scientist with the Health Professional Council (Similar to the ASCP) I am looking for a H1B Visa Sponsorship opportunity inTennessee/Knoxville or surrounding areas. I wonder if anyone can help or would be interested in having a informal discussion. I will be in Knoxville the last week September would be more then happy to travel and meet for an informal interview. Malika From flnails <@t> texaschildrens.org Thu Sep 2 12:38:40 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Sep 2 12:39:06 2010 Subject: [Histonet] RE: embedding center just did alot of research! In-Reply-To: <29A3CB81288E6F4BA2C9B3C8015A9A13024DD901@MD1EV002.medimmune.com> References: <29A3CB81288E6F4BA2C9B3C8015A9A13024DD901@MD1EV002.medimmune.com> Message-ID: Even over Tissue Tec -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Thursday, September 02, 2010 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding center just did alot of research! I did a lot of research on embedding centers and made the mistake of going with the least expensive new one. If you want to know what I bought email me separately. The prices are in the stratosphere but I have to say Leica still has the edge for this 30 plus year tech. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From Cathy.Crumpton <@t> tuality.org Thu Sep 2 14:04:01 2010 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Thu Sep 2 14:04:07 2010 Subject: [Histonet] Technical Assistant or plain Lab Assistant? Message-ID: I need help from my fellow histotechs. We are creating a po for a histology lab assistant. We need someone to assist the p athologist when grossing (not grossing themselves), clean machines, file, d this posi Lab Assistant code assistants in your area and w technical assistant, what exactly higher pay grade? This is difficult beca certification for a histology lab assistant and they do analyzers or testing machinery. Cath Tuality Community Hospital Hillsb (503)681-1292 From Margaret.Perry <@t> sdstate.edu Thu Sep 2 15:22:13 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Sep 2 15:22:19 2010 Subject: [Histonet] modified Mayers Hematoxylin Message-ID: We are looking for a procedure for modified mayer's hematoxylin. The pathologists don't like the Gills. We need something that is close to Mayer's in staining. We were told to find something without the chloral hydrate. We have found a modified Hematoxylin from Thermo that the doc's like but would like to keep costs down by making our own. Can you help us? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From sfeher <@t> CMC-NH.ORG Thu Sep 2 15:35:42 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Sep 2 15:35:48 2010 Subject: [Histonet] Technical Assistant or plain Lab Assistant? In-Reply-To: References: Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F3C3@exchange.cmc-nh.org> We created a position for a Pathology Technician. The duties are similar to those you mention with the addition of Morgue Diener. Some of our Path Techs are CAP qualified to gross small tissue specimens. In the near future we will be creating tiers to the Path Tech position, Path Tech I, Path Tech II, etc. Those qualified to gross in tissue would be graded higher than those that are not qualified to do so. Tiers could be geared towards Diener qualifications, hazardous waste handling qualifications, etc. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org Sent: Thursday, September 02, 2010 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technical Assistant or plain Lab Assistant? I need help from my fellow histotechs. We are creating a po=ition for a histology lab assistant. We need someone to assist the p athologist when grossing (not grossing themselves), clean machines, file, d=ta entry and accessioning, etc. We are trying to decide if this posi=tion should be a Technical Assistant job code or a plain Lab Assistant code=. I wanted to know if you have any such assistants in your area and w=at their category was. If it is a technical assistant, what exactly =makes the position worth the higher pay grade? This is difficult beca=use there in not a certification for a histology lab assistant and they do =ot run analyzers or testing machinery. Cath= Crumpton HT(ASCP), Histology Lead Tuality Community Hospital Hillsb=ro, OR 97123 (503)681-1292 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STACEY.LANGENBERG <@t> UCDENVER.EDU Thu Sep 2 18:23:37 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Thu Sep 2 18:25:33 2010 Subject: [Histonet] RE: embedding center just did alot of research! In-Reply-To: References: <29A3CB81288E6F4BA2C9B3C8015A9A13024DD901@MD1EV002.medimmune.com>, Message-ID: <1F70FCBB6D4EC549B2ADF69B9F9EAC0344E4EACD1B@STEAMBOAT.ucdenver.pvt> I would agree with Nicks statement on this. I love Leica and they are probably even less expensive than say Sakura. My two cents Stacey "People are not an interruption of our business. People are our business." Stacey Langenberg HT (ASCP) QIHC Laboratory Manager Histology/IF CU Dermatopathology Consultants 1999 N. Fitzsimons Pkwy Suite 120 Aurora, CO 80045 Lab-720-859-3559 Fax- 303-344-0789 Cell-970-405-7742 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton [flnails@texaschildrens.org] Sent: Thursday, September 02, 2010 11:38 AM To: 'Madary, Joseph'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: embedding center just did alot of research! Even over Tissue Tec -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Thursday, September 02, 2010 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding center just did alot of research! I did a lot of research on embedding centers and made the mistake of going with the least expensive new one. If you want to know what I bought email me separately. The prices are in the stratosphere but I have to say Leica still has the edge for this 30 plus year tech. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Sep 2 18:29:00 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Sep 2 18:29:14 2010 Subject: [Histonet] modified Mayers Hematoxylin In-Reply-To: Message-ID: Margaret, This might be of help: Garvey's Modified Mayer's Haematoxylin This solution is a progressive haematoxylin that uses 10% less alum. Chloral hydrate, because of its toxicity, has been replaced with ethanol, a more effective penetrator and bacteriostat. Mercury oxide has been replaced with sodium iodate (Garvie, (1991) J. Histotechn 14(3):164-165). Fixation: 10% buffered formalin. Microtomy: paraffin sections at 5?m. Reagents: 1. Dissolve 45g ammonium or potassium alum in 900ml distilled water with the aid of heat. 2. Dissolve 2.5g Haematoxylin (CI 75290) in 100ml absolute ethanol. 3. Combine above solutions and add 0.2g Sodium Iodate and 1g citric acid. 4. Mix well. The solution is stable for several months. Procedure: 1. Dewax and hydrate sections. 2. Stain in modified Mayer's Haematoxylin 40secs. 3. Wash in warm water 5min. 4. If required, counterstain in Eosin solution. 5. Dehydrate, clear and mount. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Friday, 3 September 2010 6:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] modified Mayers Hematoxylin We are looking for a procedure for modified mayer's hematoxylin. The pathologists don't like the Gills. We need something that is close to Mayer's in staining. We were told to find something without the chloral hydrate. We have found a modified Hematoxylin from Thermo that the doc's like but would like to keep costs down by making our own. Can you help us? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From rhbrown1 <@t> histocs.com Thu Sep 2 19:04:39 2010 From: rhbrown1 <@t> histocs.com (Leroy Brown) Date: Thu Sep 2 19:06:22 2010 Subject: [Histonet] embedding center needed Message-ID: I am looking for a "used" even well used is fine as long as it works, embedding center. I have a CBG formalin recycler that is like new I would be willing to swap. I also might be interested in a Shandon embedding center for parts if you have one that isn't too far gone. It would be for use in my histo lab not for reselling. thanks LeRoy Brown HCS, Inc www.histocs.com 360-966-7300 From napoli <@t> siscom.net Thu Sep 2 20:30:49 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Thu Sep 2 20:30:54 2010 Subject: [Histonet] OF16E59B6F.156E47E7-ON88257791.00659D15-88257791.006A0AD6@usgs.gov Message-ID: <4c804fc9.222.3265.1594981214@siscom.net> Regarding microtome blades. If you are sectioning skin, my firm opinion is that the Thermo-Shandon MX35 Premier blade is superb. Dermpath lab techs...you should try these out. They are WELL WORTH using and last a lot longer than many other types of low-profile blades. I have used them for 10 years in dermpath labs. From deannal78 <@t> verizon.net Thu Sep 2 20:36:15 2010 From: deannal78 <@t> verizon.net (Deanna Rhoads) Date: Thu Sep 2 20:36:18 2010 Subject: [Histonet] OF16E59B6F.156E47E7-ON88257791.00659D15-88257791.006A0AD6@usgs.gov In-Reply-To: <4c804fc9.222.3265.1594981214@siscom.net> References: <4c804fc9.222.3265.1594981214@siscom.net> Message-ID: <939916.57280.qm@web84301.mail.re1.yahoo.com> I agree.? I just ordered the MX35 Premier Plus and they are great.? Fisher just lowered the price of them dramatically too! ________________________________ From: Andrew Burgeson To: histonet@lists.utsouthwestern.edu Sent: Thu, September 2, 2010 9:30:49 PM Subject: [Histonet] OF16E59B6F.156E47E7-ON88257791.00659D15-88257791.006A0AD6@usgs.gov Regarding microtome blades. If you are sectioning skin, my firm opinion is that the Thermo-Shandon MX35 Premier blade is superb. Dermpath lab techs...you should try these out. They are WELL WORTH using and last a lot longer than many other types of low-profile blades. I have used them for 10 years in dermpath labs. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Fri Sep 3 07:05:10 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Sep 3 07:05:21 2010 Subject: [Histonet] Hazardous waste In-Reply-To: Message-ID: <8BC7B0EED50B497C906668904E3C9ED1@hopperPC> We are using a solvent recycler and have no longer have any xylene waste to haul away. :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Thursday, September 02, 2010 10:30 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Hazardous waste Histonetters We are just now keeping a count of how many gallons we dispose of because the company that hauls it off gave us a 55 gal drum and charges us for 55 gal drum everytime he picks it up so we are going for a count for one month to see how much we dispose uf and maybe get a smaller drum so not to have to pay as much. Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3104 - Release Date: 08/31/10 06:34:00 From brandihiggins <@t> gmail.com Fri Sep 3 08:03:28 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Fri Sep 3 08:03:32 2010 Subject: [Histonet] LI pathology lab looking for PA for grossing Message-ID: Hello all, We are a small pathology lab located on the east end of Long Island. We are looking for a PA to do part time grossing. If you know any one that is interested please have them email me. Thanks, Brandi Higgins, BS, HT(ASCP) From jaylundgren <@t> gmail.com Fri Sep 3 13:59:32 2010 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Sep 3 13:59:37 2010 Subject: [Histonet] Ventana vs. Leica Part II : The Revenge Message-ID: I want to thank all of you for your helpful input on my Leica vs Ventana question. In my opinion, this is exactly what Histonet is best at, getting unbiased opinions from the people who are actually in the trenches. By the way, the Ventana rep actually called the histology supervisor here at 7:20 A.M. to try to refute one of the claims. 0720 isn't very early for a histotech, but it's pretty early for a sales rep. Does anyone else have any stories of vendor hijinks to share? No vendors please. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From hymclab.hymclab <@t> ministryhealth.org Fri Sep 3 14:33:07 2010 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Fri Sep 3 14:33:29 2010 Subject: [Histonet] OF16E59B6F.156E47E7-ON88257791.00659D15-88257791.006A0AD6@usgs.gov In-Reply-To: <4c804fc9.222.3265.1594981214@siscom.net> References: <4c804fc9.222.3265.1594981214@siscom.net> Message-ID: We use these for everything a love them!!!! They seem to last the longest in addition to cutting great!!! Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson Sent: Thursday, September 02, 2010 8:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OF16E59B6F.156E47E7-ON88257791.00659D15-88257791.006A0AD6@usgs.gov Regarding microtome blades. If you are sectioning skin, my firm opinion is that the Thermo-Shandon MX35 Premier blade is superb. Dermpath lab techs...you should try these out. They are WELL WORTH using and last a lot longer than many other types of low-profile blades. I have used them for 10 years in dermpath labs. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Maria.Katleba <@t> stjoe.org Fri Sep 3 15:03:40 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Sep 3 15:04:09 2010 Subject: [Histonet] Caris and Genoptix hardballing pathology labs In-Reply-To: References: Message-ID: Good one Jay! I have had issue with a couple reference labs... Yes... I had a Caris Life Science rep show up in blue scrubs (just like our facility). He misleads me into believing he was with the oncology group. So I took time out of day to speak to him (thinking he was a Queen of the Valley employee, nurse or tech). You can imagine how very annoyed I was to find out that he just dressed up like this because it gives him a better chance to wedge through the door of a lab. Next is Genoptix... long story short: Genoptix faxed a request for blocks, reports, and slides so that they could perform a test for an oncologist... I have never heard of these people, never seen a rep, nor do I even have a CLIA certificate on file, and why do they want 'everything on file" Seriously? So I call them. Come to find out, our current lab of choice does the same test. Not to mention the courier was due to arrive in 45minutes to pick up our send outs. I spoke to the customer service person at Genoptix... she was going to fax me a CLIA... so I decided to check with my pathologists. The block went to our longstanding reference lab and the case was done quickly. A few days later, I get a few inappropriate calls from Genoptix. They were very angry that they lost business because I did not send them the case. They also made sure to tell the oncologists that "the pathology lab refused to send the block..." They went on further to suggest that I was sending the block to our current ref. lab because of some "kick backs".... Let me assure everyone on Histonet... KICK BACKS ARE ILLEGAL! That being said, every reference lab we have ever used does not participate in kickbacks... Genoptix though, that's another story. Pitting an oncology group against the hospital's pathology lab is a very poor way of getting the business. Those tactics don't go over well with any of us! Caris Life Science and Genoptix have a lot to learn... Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Friday, September 03, 2010 12:00 PM To: histonet Subject: [Histonet] Ventana vs. Leica Part II : The Revenge I want to thank all of you for your helpful input on my Leica vs Ventana question. In my opinion, this is exactly what Histonet is best at, getting unbiased opinions from the people who are actually in the trenches. By the way, the Ventana rep actually called the histology supervisor here at 7:20 A.M. to try to refute one of the claims. 0720 isn't very early for a histotech, but it's pretty early for a sales rep. Does anyone else have any stories of vendor hijinks to share? No vendors please. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Bill.Tench <@t> pph.org Fri Sep 3 15:13:22 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Sep 3 15:13:27 2010 Subject: [Histonet] caris and genoptix experience Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A55B8@MAIL1.pph.local> We have had almost identical experiences with both organizations. Caris has been informed that it is not welcome; genoptix is on the same list Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From vjp2105 <@t> columbia.edu Fri Sep 3 15:26:31 2010 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Fri Sep 3 15:26:38 2010 Subject: [Histonet] TUNEL Message-ID: Hi everyone, I am hoping you can help me I am looking for some advice on how to do TUNEL staining (fluorescent or enzymatic), kits recommended/ protocol. Any help would be greatly appreciated. Thanking you in advance From liz <@t> premierlab.com Fri Sep 3 15:35:47 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Sep 3 15:34:26 2010 Subject: [Histonet] TUNEL In-Reply-To: Message-ID: We use the Roche Kit with good success. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa J. Phelan Sent: Friday, September 03, 2010 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TUNEL Hi everyone, I am hoping you can help me I am looking for some advice on how to do TUNEL staining (fluorescent or enzymatic), kits recommended/ protocol. Any help would be greatly appreciated. Thanking you in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Fri Sep 3 16:27:14 2010 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Fri Sep 3 16:27:30 2010 Subject: [Histonet] Cryostat decontamination Message-ID: <8CD19C5E975CD69-1950-4E6E@webmail-d042.sysops.aol.com> Can someone send me a procedure on how to decontaminate a cryostat? Thank you, Ann From macveigh <@t> usc.edu Fri Sep 3 18:51:46 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Fri Sep 3 18:51:49 2010 Subject: [Histonet] TUNEL Message-ID: We also use Roche kits. The cat # 11 684 795 910 is for fluorescent and cat# 11 684 817 910 is for loight microscope. There are aditional chemicals to purchase, beside the kit. Call Roche for more info. Good luck! Michelle USC School of Medicine From histotech <@t> imagesbyhopper.com Fri Sep 3 21:51:45 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Sep 3 21:51:49 2010 Subject: [Histonet] caris and genoptix experience In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A55B8@MAIL1.pph.local> Message-ID: Very interesting. I recently had a very pushy Genoptix rep at my facility. Wanted to take a LOT of my time, but as I was walking out the door (the rep came unannounced), they only got about 10 minutes. They were quick to point out that wasn't enough time ... well, um ... make an appointment!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, September 03, 2010 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] caris and genoptix experience We have had almost identical experiences with both organizations. Caris has been informed that it is not welcome; genoptix is on the same list Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3104 - Release Date: 09/03/10 06:34:00 From Kim.Donadio <@t> bhcpns.org Sat Sep 4 10:55:18 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Sat Sep 4 10:55:42 2010 Subject: [Histonet] caris and genoptix experience In-Reply-To: Message-ID: I had exactly the same experience with the same company. It's a bad mark right off the bat with me if vendors just walk in the door and expect me to drop what I am doing. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-bounces@lists.utsouthwestern.edu 09/03/2010 09:51 PM To "'Tench, Bill'" , cc Subject RE: [Histonet] caris and genoptix experience Very interesting. I recently had a very pushy Genoptix rep at my facility. Wanted to take a LOT of my time, but as I was walking out the door (the rep came unannounced), they only got about 10 minutes. They were quick to point out that wasn't enough time ... well, um ... make an appointment!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, September 03, 2010 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] caris and genoptix experience We have had almost identical experiences with both organizations. Caris has been informed that it is not welcome; genoptix is on the same list Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3104 - Release Date: 09/03/10 06:34:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From DianaRip1 <@t> aol.com Sat Sep 4 19:06:41 2010 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Sat Sep 4 19:06:53 2010 Subject: [Histonet] accu-edge low profile microtome blades Message-ID: <2027ee.609bd825.39b43911@aol.com> We've always used them too. They've always had oil on them. We just dip them in alcohol and then xylene to take the coating off. I try to not estimate more than I will use in a day. If you take the coating off and don't cover them with a paper towel at night they will rust. From carolina.soekmadji <@t> qut.edu.au Sat Sep 4 19:14:28 2010 From: carolina.soekmadji <@t> qut.edu.au (Carolina Soekmadji) Date: Sat Sep 4 19:14:36 2010 Subject: [Histonet] cryosection for IHC samples Message-ID: <60495E34E2CF58448A44DE567C6FAF240BC7CA7A71@QUTEXMBX03.qut.edu.au> Hi, Could anybody give me an advice about sample processing for cryosection? For best IHC, is it better to go with fresh tumour sample that goes straight to cryosection, then fix with PFA ? How long we need to fix in PFA in this case? 30 min? Or is it better to process it by fixing tumour first by immersion in PFA then sucrose then cryosection ? Best wishes, Carolina Carolina Soekmadji, PhD. MSc. Postdoctoral Research Fellow Australian Prostate Cancer Research Centre - Queensland | Institute of Health and Biomedical Innovation -QUT | t: 07 3176 3099 (APCRC - Q, Princess Alexandra Hospital) t: 07 3138 6286 (IHBI, QUT Kelvin Grove) mobile: +61 423 111 807 f: 07 3176 7440 e: carolina.soekmadji@qut.edu.au From itai.moshe <@t> mail.huji.ac.il Sun Sep 5 07:37:59 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Sun Sep 5 07:45:14 2010 Subject: [Histonet] Problem with liver fixation Message-ID: Dear All, I'm trying to use liver sections in paraffin blocks for IHC, but get no signal at all, doesn't matter what antibody i'm using. I'm using section about 3mm thick (the natural thickness of mouse liver) and about 5-7mm Width and Length. My fixation protocol is like this: 1) immediately after killing the mouse i'm putting the sections in a fixation solution that is made from: 10ml formaldehyde 37%, 5ml PBSx20, 85ml DDW - pH 7, 24Hr at 4C. 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT 3) Xylen x2 - each for 1Hr at RT. 4) Paraffin x3 - each at 60C for 1Hr. Before doing IHC: 1) Xylen x2 - each for 10Min at RT 2) ETOH 100%x2, ETOH 96%, ETOH 80%, ETOH 70% - each for 2Min at RT. 3) IHC protocol... Will Bouin's solution be better ? Thank you all very much in advance Itai M From rjbuesa <@t> yahoo.com Sun Sep 5 08:14:08 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Sep 5 08:14:12 2010 Subject: [Histonet] Problem with liver fixation In-Reply-To: Message-ID: <607770.91617.qm@web65704.mail.ac4.yahoo.com> Bouin's will not do better than what you describe. Are you using antigen retrieval? Ren? J. --- On Sun, 9/5/10, Itai Moshe wrote: From: Itai Moshe Subject: [Histonet] Problem with liver fixation To: histonet@lists.utsouthwestern.edu Date: Sunday, September 5, 2010, 8:37 AM Dear All, I'm trying to use liver sections in paraffin blocks for IHC, but get no signal at all, doesn't matter what antibody i'm using. I'm using section about 3mm thick (the natural thickness of mouse liver) and about 5-7mm Width and Length. My fixation protocol is like this: 1) immediately after killing the mouse i'm putting the sections in a fixation solution that is made from: 10ml formaldehyde 37%, 5ml PBSx20, 85ml DDW? - pH 7, 24Hr at 4C. 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT 3) Xylen x2 - each for 1Hr at RT. 4) Paraffin x3 - each at 60C for 1Hr. Before doing IHC: 1) Xylen x2 - each for 10Min at RT 2) ETOH 100%x2, ETOH 96%, ETOH 80%, ETOH 70% - each for 2Min at RT. 3) IHC protocol... Will Bouin's solution be better ? Thank you all very much in advance Itai M _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Sep 5 13:19:43 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Sep 5 13:20:27 2010 Subject: SPAM-LOW: [Histonet] cryosection for IHC samples In-Reply-To: <60495E34E2CF58448A44DE567C6FAF240BC7CA7A71@QUTEXMBX03.qut.edu.au> References: <60495E34E2CF58448A44DE567C6FAF240BC7CA7A71@QUTEXMBX03.qut.edu.au> Message-ID: It depends on the marker you are using. Some antibodies require acetone/ethanol fixation and are not recommended to be used with aldehyde fixatives. If you know your marker works with PFA fixative why not fix it and then process it into paraffin. I use frozen sections just for tissues and markers that may not do well with aldehyde fixation and paraffin processing. I use fixed tissues that are then infiltrated in 30% sucrose mostly for tissues that will be difficult to cut, such as calcified bone and then use a tape transfer sectioning technique. Since you are working with tumor you probably do not need to fix and then cyroprotect in sucrose as the unfixed snap frozen sections should section pretty well directly. Decide from there if you need to fix in acetone or PFA (depending on what ab you are using). Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carolina Soekmadji Sent: Saturday, September 04, 2010 6:14 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] cryosection for IHC samples Hi, Could anybody give me an advice about sample processing for cryosection? For best IHC, is it better to go with fresh tumour sample that goes straight to cryosection, then fix with PFA ? How long we need to fix in PFA in this case? 30 min? Or is it better to process it by fixing tumour first by immersion in PFA then sucrose then cryosection ? Best wishes, Carolina Carolina Soekmadji, PhD. MSc. Postdoctoral Research Fellow Australian Prostate Cancer Research Centre - Queensland | Institute of Health and Biomedical Innovation -QUT | t: 07 3176 3099 (APCRC - Q, Princess Alexandra Hospital) t: 07 3138 6286 (IHBI, QUT Kelvin Grove) mobile: +61 423 111 807 f: 07 3176 7440 e: carolina.soekmadji@qut.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Sep 5 13:25:38 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Sep 5 13:26:20 2010 Subject: SPAM-LOW: [Histonet] Problem with liver fixation In-Reply-To: References: Message-ID: <08E669C957BA4660A118D4B3D66B78E6@prueggihctechlt> I would not fix at 4c, do it at rt. Your fixative looks ok and processing, actually for animal tissues I process for only 20 min a station not 60 min., animal tissues will process quicker because they have less fat, and you can over process them so they become dryed out and hard. About not getting a signal no matter what ab you use, there could be several causes, are you using the proper antigen retrieval technique for that ab, is your detection system matched to your primary antibody, on and on..........? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Itai Moshe Sent: Sunday, September 05, 2010 6:38 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Problem with liver fixation Dear All, I'm trying to use liver sections in paraffin blocks for IHC, but get no signal at all, doesn't matter what antibody i'm using. I'm using section about 3mm thick (the natural thickness of mouse liver) and about 5-7mm Width and Length. My fixation protocol is like this: 1) immediately after killing the mouse i'm putting the sections in a fixation solution that is made from: 10ml formaldehyde 37%, 5ml PBSx20, 85ml DDW - pH 7, 24Hr at 4C. 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT 3) Xylen x2 - each for 1Hr at RT. 4) Paraffin x3 - each at 60C for 1Hr. Before doing IHC: 1) Xylen x2 - each for 10Min at RT 2) ETOH 100%x2, ETOH 96%, ETOH 80%, ETOH 70% - each for 2Min at RT. 3) IHC protocol... Will Bouin's solution be better ? Thank you all very much in advance Itai M _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sun Sep 5 16:08:43 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Sep 5 16:08:47 2010 Subject: [Histonet] Tunel Message-ID: Hi, I love the Millipore kit S7101. The data sheet (instructions) require a bit of distilling into a palatable form, but it works great once you clean up the procedure some. If you need a hand with this one drop me a line. I do it very often. Incidentally as with most kits some parts end up used up faster than others. Millipore sells the individual components as well so no sweat there. Good luck, Amos Message: 5 Date: Fri, 3 Sep 2010 16:26:31 -0400 From: "Vanessa J. Phelan" Subject: [Histonet] TUNEL To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi everyone, I am hoping you can help me I am looking for some advice on how to do TUNEL staining (fluorescent or enzymatic), kits recommended/ protocol. Any help would be greatly appreciated. Thanking you in advance From alisha <@t> ka-recruiting.com Mon Sep 6 15:57:08 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Mon Sep 6 15:57:06 2010 Subject: [Histonet] Unique Histotech Jobs Message-ID: <684567943.1283806627930.JavaMail.cfservice@SL4APP4> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working with a client just outside of Indianapolis, IN. This hospital has a very strong reputation in Indiana and has repeatedly won awards for being one of the best places to work in the state! My client is currently expanding their laboratory and looking for a Histotechn, with 5+ years experience. This is a day shift position. This hospital system offers a very competitive base salary/hourly rate, shift differentials, and an outstanding benefits and retirement package. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Histotech Opportunities: IN - Histotech 1st shift GA - Histology Supervisor - 1st shift MA - Histotech - 1st shift MI - Histology Manager - 1st shift NY - Histotech 3rd shift NV - Histotech 3rd shift (must have Bachelor's Degree) FL - Histotechnologist with IHC experience (5+ years) NJ - Anatomic Pathology General Manager of Operations If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From amario3 <@t> uic.edu Mon Sep 6 21:01:50 2010 From: amario3 <@t> uic.edu (Andrea Marion) Date: Mon Sep 6 21:01:59 2010 Subject: [Histonet] Re: Problem with liver fixation Message-ID: Hi Itai, Liver antigens tend to degrade rapidly, so immediate fixation is necessary. It sounds like you are getting the tissue into the solution quickly enough. Fixation occurs more slowly at 4 degrees than room temperature though - perhaps your antigen is degrading during this 'slower' fixation? Are you using a sufficient volume of fixative compared to tissue mass? Another concern is that some antigens require paraformaldehyde fixation (ie a 4% solution of buffered formaldehyde prepared directly from solid paraformaldehyde). Solutions of formaldehyde marketed as '37% formaldehyde' or formalin typically contain ~10% methanol added as a stabilizer, which can interfere with some antigens. For immunohistochemistry purposes, you may need to prepare your formaldehyde solution directly from paraformaldehye. See here for more discussion of formaldehyde solutions: http://publish.uwo.ca/~jkiernan/formglut.htm Finally, you list 'IHC protocol', but why are you sure your problem lies in the tissue fixation/processing and not the staining protocol? You probably need to do some form of antigen retrieval - what method are you using? What is your primary antibody dilution? Has your antibody been validated for use with IHC/IF? Many antibodies simply do not work with FFPE tissues. There are many other steps that could be causing trouble... Here is a good beginner's guide for IHC with FFPE tissues if you need: http://www.abcam.com/ps/pdf/protocols/ihc_p.pdf As a side note, your processing steps may be a little long, but I am not an expert. We use only 30 minutes for each dehydration and clearing step, and 2 paraffin steps for one hour each with mouse tissue. Good luck! Andrea Marion amario3 &at& uic &dot& edu Graduate Student University of Illinois at Chicago From adesupo2002 <@t> hotmail.com Mon Sep 6 22:27:43 2010 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Mon Sep 6 22:27:49 2010 Subject: [Histonet] GEN. 55500 In-Reply-To: References: Message-ID: Hi, Does anyone have a competency checklist for Histotechs that they would like to share? Besides can we use thesame competency checklist for Histotechnologist (HTL), Histotechnician(HT) and Histotechnician(Non Registered)? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). From itai.moshe <@t> mail.huji.ac.il Tue Sep 7 04:13:24 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Tue Sep 7 04:14:16 2010 Subject: [Histonet] Re: Problem with liver fixation In-Reply-To: References: Message-ID: Thank's all, I'm thinking that my IHC method might be O.K because it is working great with other paraffin embeddedd tissues like diaphragm and various muscles, and (sometimes) also in older liver tissues that i have found in our lab (but i don't know how they were made). I'm using antigen retrieval with 0.01% Citrate buffer, pH 6.0 heated in microwave. but I don't rule out that my IHC method is not optimal for liver tissues, and that there might be a better one. Does someone have a proven fixation, or IHC methods that worked with live tissues ? Thank you all very much Itai M 2010/9/7 Andrea Marion > Hi Itai, > > Liver antigens tend to degrade rapidly, so immediate fixation is > necessary. It sounds like you are getting the tissue into the solution > quickly enough. Fixation occurs more slowly at 4 degrees than room > temperature though - perhaps your antigen is degrading during this > 'slower' fixation? Are you using a sufficient volume of fixative compared > to tissue mass? > > Another concern is that some antigens require paraformaldehyde fixation > (ie a 4% solution of buffered formaldehyde prepared directly from solid > paraformaldehyde). Solutions of formaldehyde marketed as '37% > formaldehyde' or formalin typically contain ~10% methanol added as a > stabilizer, which can interfere with some antigens. For > immunohistochemistry purposes, you may need to prepare your formaldehyde > solution directly from paraformaldehye. See here for more discussion of > formaldehyde solutions: http://publish.uwo.ca/~jkiernan/formglut.htm > > Finally, you list 'IHC protocol', but why are you sure your problem lies > in the tissue fixation/processing and not the staining protocol? You > probably need to do some form of antigen retrieval - what method are you > using? What is your primary antibody dilution? Has your antibody been > validated for use with IHC/IF? Many antibodies simply do not work with > FFPE tissues. There are many other steps that could be causing trouble... > Here is a good beginner's guide for IHC with FFPE tissues if you need: > http://www.abcam.com/ps/pdf/protocols/ihc_p.pdf > > As a side note, your processing steps may be a little long, but I am not > an expert. We use only 30 minutes for each dehydration and clearing step, > and 2 paraffin steps for one hour each with mouse tissue. Good luck! > > Andrea Marion > > amario3 &at& uic &dot& edu > Graduate Student > University of Illinois at Chicago > > > ---- > Rene J Buesa histonet@lists.utsouthwestern.edu ?, Itai Moshe Bouin's will not do better than what you describe. Are you using antigen retrieval? Ren? J. ---- Patsy Ruegg Itai Moshe itai.moshe@mail.huji.ac.il ?, I would not fix at 4c, do it at rt. Your fixative looks ok and processing, actually for animal tissues I process for only 20 min a station not 60 min., animal tissues will process quicker because they have less fat, and you can over process them so they become dryed out and hard. About not getting a signal no matter what ab you use, there could be several causes, are you using the proper antigen retrieval technique for that ab, is your detection system matched to your primary antibody, on and on..........? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org --- Itai Moshe ?? histonet@lists.utsouthwestern.edu Dear All, I'm trying to use liver sections in paraffin blocks for IHC, but get no signal at all, doesn't matter what antibody i'm using. I'm using section about 3mm thick (the natural thickness of mouse liver) and about 5-7mm Width and Length. My fixation protocol is like this: 1) immediately after killing the mouse i'm putting the sections in a fixation solution that is made from: 10ml formaldehyde 37%, 5ml PBSx20, 85ml DDW - pH 7, 24Hr at 4C. 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT 3) Xylen x2 - each for 1Hr at RT. 4) Paraffin x3 - each at 60C for 1Hr. Before doing IHC: 1) Xylen x2 - each for 10Min at RT 2) ETOH 100%x2, ETOH 96%, ETOH 80%, ETOH 70% - each for 2Min at RT. 3) IHC protocol... Will Bouin's solution be better ? Thank you all very much in advance Itai M From itai.moshe <@t> mail.huji.ac.il Tue Sep 7 04:13:24 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Tue Sep 7 04:19:10 2010 Subject: [Histonet] Re: Problem with liver fixation In-Reply-To: References: Message-ID: Thank's all, I'm thinking that my IHC method might be O.K because it is working great with other paraffin embeddedd tissues like diaphragm and various muscles, and (sometimes) also in older liver tissues that i have found in our lab (but i don't know how they were made). I'm using antigen retrieval with 0.01% Citrate buffer, pH 6.0 heated in microwave. but I don't rule out that my IHC method is not optimal for liver tissues, and that there might be a better one. Does someone have a proven fixation, or IHC methods that worked with live tissues ? Thank you all very much Itai M 2010/9/7 Andrea Marion > Hi Itai, > > Liver antigens tend to degrade rapidly, so immediate fixation is > necessary. It sounds like you are getting the tissue into the solution > quickly enough. Fixation occurs more slowly at 4 degrees than room > temperature though - perhaps your antigen is degrading during this > 'slower' fixation? Are you using a sufficient volume of fixative compared > to tissue mass? > > Another concern is that some antigens require paraformaldehyde fixation > (ie a 4% solution of buffered formaldehyde prepared directly from solid > paraformaldehyde). Solutions of formaldehyde marketed as '37% > formaldehyde' or formalin typically contain ~10% methanol added as a > stabilizer, which can interfere with some antigens. For > immunohistochemistry purposes, you may need to prepare your formaldehyde > solution directly from paraformaldehye. See here for more discussion of > formaldehyde solutions: http://publish.uwo.ca/~jkiernan/formglut.htm > > Finally, you list 'IHC protocol', but why are you sure your problem lies > in the tissue fixation/processing and not the staining protocol? You > probably need to do some form of antigen retrieval - what method are you > using? What is your primary antibody dilution? Has your antibody been > validated for use with IHC/IF? Many antibodies simply do not work with > FFPE tissues. There are many other steps that could be causing trouble... > Here is a good beginner's guide for IHC with FFPE tissues if you need: > http://www.abcam.com/ps/pdf/protocols/ihc_p.pdf > > As a side note, your processing steps may be a little long, but I am not > an expert. We use only 30 minutes for each dehydration and clearing step, > and 2 paraffin steps for one hour each with mouse tissue. Good luck! > > Andrea Marion > > amario3 &at& uic &dot& edu > Graduate Student > University of Illinois at Chicago > > > ---- > Rene J Buesa histonet@lists.utsouthwestern.edu ?, Itai Moshe Bouin's will not do better than what you describe. Are you using antigen retrieval? Ren? J. ---- Patsy Ruegg Itai Moshe itai.moshe@mail.huji.ac.il ?, I would not fix at 4c, do it at rt. Your fixative looks ok and processing, actually for animal tissues I process for only 20 min a station not 60 min., animal tissues will process quicker because they have less fat, and you can over process them so they become dryed out and hard. About not getting a signal no matter what ab you use, there could be several causes, are you using the proper antigen retrieval technique for that ab, is your detection system matched to your primary antibody, on and on..........? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org --- Itai Moshe ?? histonet@lists.utsouthwestern.edu Dear All, I'm trying to use liver sections in paraffin blocks for IHC, but get no signal at all, doesn't matter what antibody i'm using. I'm using section about 3mm thick (the natural thickness of mouse liver) and about 5-7mm Width and Length. My fixation protocol is like this: 1) immediately after killing the mouse i'm putting the sections in a fixation solution that is made from: 10ml formaldehyde 37%, 5ml PBSx20, 85ml DDW - pH 7, 24Hr at 4C. 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT 3) Xylen x2 - each for 1Hr at RT. 4) Paraffin x3 - each at 60C for 1Hr. Before doing IHC: 1) Xylen x2 - each for 10Min at RT 2) ETOH 100%x2, ETOH 96%, ETOH 80%, ETOH 70% - each for 2Min at RT. 3) IHC protocol... Will Bouin's solution be better ? Thank you all very much in advance Itai M From mcauliff <@t> umdnj.edu Tue Sep 7 08:26:55 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Sep 7 08:24:24 2010 Subject: [Histonet] Problem with liver fixation In-Reply-To: References: Message-ID: <4C863D9F.6020800@umdnj.edu> Formalin fixes slowly, even more slowly at 4 degrees C. Even though the fix may penetrate, fixation will not be complete. I suggest a formalin+alcohol+acetic acid fix at room temp for 24 hours. There are many, many variations of this fix. Lillie recommends 85 ml of 95% EtOH, 10 ml conc. formalin (37-40% formaldehyde), 5 ml glacial acetic acid. I agree with Rene, Bouin will not make a difference. Alchoholic Bouin is also worth a try. Geoff Itai Moshe wrote: > Dear All, > I'm trying to use liver sections in paraffin blocks for IHC, but get no > signal at all, doesn't matter what antibody i'm using. > > I'm using section about 3mm thick (the natural thickness of mouse liver) and > about 5-7mm Width and Length. > > My fixation protocol is like this: > 1) immediately after killing the mouse i'm putting the sections in a > fixation solution that is made from: 10ml formaldehyde 37%, 5ml PBSx20, 85ml > DDW - pH 7, 24Hr at 4C. > 2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT > 3) Xylen x2 - each for 1Hr at RT. > 4) Paraffin x3 - each at 60C for 1Hr. > > Before doing IHC: > 1) Xylen x2 - each for 10Min at RT > 2) ETOH 100%x2, ETOH 96%, ETOH 80%, ETOH 70% - each for 2Min at RT. > 3) IHC protocol... > > Will Bouin's solution be better ? > > Thank you all very much in advance > > Itai M > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From amkmadden <@t> gmail.com Tue Sep 7 11:10:27 2010 From: amkmadden <@t> gmail.com (Amanda Madden) Date: Tue Sep 7 11:11:06 2010 Subject: [Histonet] Losing Sections While Dehydrating Message-ID: Hello Everyone! I have searched the archives because I am sure this is a problem that has been encountered before, but I have been unsuccessful in finding the right thread. I ran an IHC series dilution on a new primary antibody for NeuN. I am using 35 um rat brain sections that were cut on a cryostat and stored in Cryoprotectant until ICC began. After finishing the ICC protocol, I mounted the slides that were floating in phosphate buffered saline onto slides that I subbed two weeks ago (.5% gelatin subbing). I allowed the slides to dry from Friday afternoon until Tuesday afternoon. I tried to dehydrate them for coverslipping and when they went into the dH20 bath, some of the sections began to fall off of the slides. This continued in the 50% Alcohol bath. All tissue that made it through those first two baths in the series stayed on throughout, but I did lose quite a few sections, especially those that had the lowest concentration of primary antibody. My experience has been that highly concentrated primaries make the tissue a bit sticky, but the best staining generally comes from the least concentrated. Does anyone know what the problem might be? I think it might be a subbing problem but I'm not sure. Thanks in advance. Amanda Madden Research Assistant P.S. The subbing protocol that I used only called for one "dip" into the gelatin solution, and didn't call for any type of acid wash. Also, our cryostat is a Leica CM3050S and I was wondering if any of your labs have established a working range in terms of humidity. Our lab has been experiencing very high humidity levels (up to almost 80%), so we were wondering if anyone has established that cutting cannot be done unless the humidity is under x%. From emerald_lake77 <@t> yahoo.com Tue Sep 7 14:47:26 2010 From: emerald_lake77 <@t> yahoo.com (Robert Hughes) Date: Tue Sep 7 14:47:30 2010 Subject: [Histonet] Negative Control Tissue - No / Few Macrophages Message-ID: <671612.48727.qm@web110606.mail.gq1.yahoo.com> Hello, ? Does anyone know of a good control tissue absent of (or with few)?macrophages? ? I have seen a number of postings regarding tissue positive for various inflammatory markers (F4/80, MOMA. CD68). ? I am running F4/80 (Serotec, Rat anti-ms F4/80) on ms. tissue.? I have a number of positive control tissues -- liver, spleen, etc. ? Any suggestions would be very helpful. ? Thank you. ? Gustave ? Gustave T. Hebert Research Scientist I Pfizer Cambridge, MA 02140 ? ? From klauing <@t> lumc.edu Tue Sep 7 15:35:03 2010 From: klauing <@t> lumc.edu (Kristen Lauing) Date: Tue Sep 7 15:35:13 2010 Subject: [Histonet] Rat tendon processing Message-ID: <4C865AB8.32C2.00FD.1@lumc.edu> Hi, I am processing rat infraspinatus tendons for Masson Trichrome staining. I've had trouble with routine processing protocols and read that adding 4% phenol to the 95% alcohol solutions may help soften the tissue to make it easier to cut. Has anyone ever tried this, or have other suggestions for preventing the tendon from getting so brittle it falls out of the paraffin? Also, should I be extending the time that the tendon spends in each alcohol station or shortening it? I am processing manually since we do not have access to an automatic processor. Thanks, Kristen From Vickroy.Jim <@t> mhsil.com Tue Sep 7 15:44:47 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Sep 7 15:44:54 2010 Subject: [Histonet] trephine protocols Message-ID: <24A4826E8EF0964D86BC5317306F58A554D2B4CD89@mmc-mail.ad.mhsil.com> We are experiencing some problems with our trephine bone marrow biopsies. Could anyone share with me the amount of time they are generally using for decalcification and what reagents you are using? We are also experiencing fragmentation in some of the slides. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From CIngles <@t> uwhealth.org Tue Sep 7 15:46:37 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Sep 7 15:48:33 2010 Subject: [Histonet] caris and genoptix experience References: Message-ID: Gee... It's not like WE have work to do or anything. Thanks for the heads up about these companies. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim.Donadio@bhcpns.org Sent: Sat 9/4/2010 10:55 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] caris and genoptix experience I had exactly the same experience with the same company. It's a bad mark right off the bat with me if vendors just walk in the door and expect me to drop what I am doing. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-bounces@lists.utsouthwestern.edu 09/03/2010 09:51 PM To "'Tench, Bill'" , cc Subject RE: [Histonet] caris and genoptix experience Very interesting. I recently had a very pushy Genoptix rep at my facility. Wanted to take a LOT of my time, but as I was walking out the door (the rep came unannounced), they only got about 10 minutes. They were quick to point out that wasn't enough time ... well, um ... make an appointment!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, September 03, 2010 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] caris and genoptix experience We have had almost identical experiences with both organizations. Caris has been informed that it is not welcome; genoptix is on the same list Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3104 - Release Date: 09/03/10 06:34:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. 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Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Tue Sep 7 16:15:44 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Sep 7 16:15:53 2010 Subject: [Histonet] Silver In-Reply-To: References: Message-ID: I know... I should wear gloves when doing a GMS.....I... know... that. (sorry, I thought this was Facebook for a second) Have a great week! - Sir Bill of the Blackened Thumb From CIngles <@t> uwhealth.org Tue Sep 7 17:57:18 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Sep 7 17:59:08 2010 Subject: [Histonet] Silver References: Message-ID: I thought Histotechs were supposed to have purple thumbs. :) (You know, gardeners have green thumbs...) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of O'Donnell, Bill Sent: Tue 9/7/2010 4:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver I know... I should wear gloves when doing a GMS.....I... know... that. (sorry, I thought this was Facebook for a second) Have a great week! - Sir Bill of the Blackened Thumb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Tue Sep 7 18:31:25 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Sep 7 18:32:09 2010 Subject: [Histonet] FW: Ventana vs. Leica Message-ID: This is how professional histotechs treat each other? A very sad commentary in a country where people are afforded free speech. Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime ________________________________ From: Joe Jones [mailto:joe_j_jones99@yahoo.com] Sent: Tuesday, September 07, 2010 4:28 PM To: Maria Katleba Subject: Ventana vs. Leica Maria, Please stop embarassing those of us who know you and have to work with you. You are clueless about Ventana's business, let alone anyone elses. Your postings on Histonet scream "idiot", so please stop. Ventana paying for a company's waste has nothing to do with a business plan, you dim ass. They pay it after some customer's bring up the additional cost when weighing their options. Fix the "machine" (it's not a machine you stupid tool)? If you knew anything about how instrument's are designed, you'd realize a company doesn't go back and redo an instrument. Now shut up and do us all a favor. Toni brings up a very good point.... you know it's pretty bad when a company is willing to pay your waste costs... Not a good business plan. Why not 'fix' the machine so that it's more "green" Maria Katleba MS HT(ASCP) ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From rgrow <@t> bmnet.com Wed Sep 8 07:10:12 2010 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Wed Sep 8 07:10:15 2010 Subject: [Histonet] Position Open in E. Tennessee Message-ID: Blount Memorial Hospital in Maryville, TN still has a full time histology technician position open Monday thru Friday. If you are interested in working for a hospital that cares about its patients, we may be what you are looking for. General histology experience with certification preferred. Some Immunohistochemistry would be helpful. Occasional variance in schedule may be necessary as needed. Must demonstrate competency and successfully complete the on-the-job orientation through the histology section of the laboratory. Perform all duties of a Histology Technician and other duties as assigned. Applicants must meet the educational and training requirements necessary for certification by the American Society of Clinical Pathology as a Histology Technician or have experience equal to certification. Good benefits are offered. Anyone interested please visit our website at blountmemorial.org. to fill out an application and attach a resume. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From cdemarinis <@t> SARATOGACARE.ORG Wed Sep 8 08:21:41 2010 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Wed Sep 8 08:21:49 2010 Subject: [Histonet] Pathology billing for consultation Message-ID: I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From flnails <@t> texaschildrens.org Wed Sep 8 09:11:29 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Sep 8 09:11:47 2010 Subject: [Histonet] RE: Ventana vs. Leica In-Reply-To: References: Message-ID: There are many opinions on the histonet that I disagree with, however it is just an opinion and no one should be disrespected how joe jones decided to disrespect Maria. Lets all remember it is just an opinion and someone asked for it. Hopefully Joe Jones is not an employee of Ventana because it would definitely sway my opinion about who they hire and how the treat potential clients. Just my opinion. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Tuesday, September 07, 2010 6:31 PM To: histonet Subject: [Histonet] FW: Ventana vs. Leica This is how professional histotechs treat each other? A very sad commentary in a country where people are afforded free speech. Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime ________________________________ From: Joe Jones [mailto:joe_j_jones99@yahoo.com] Sent: Tuesday, September 07, 2010 4:28 PM To: Maria Katleba Subject: Ventana vs. Leica Maria, Please stop embarassing those of us who know you and have to work with you. You are clueless about Ventana's business, let alone anyone elses. Your postings on Histonet scream "idiot", so please stop. Ventana paying for a company's waste has nothing to do with a business plan, you dim ass. They pay it after some customer's bring up the additional cost when weighing their options. Fix the "machine" (it's not a machine you stupid tool)? If you knew anything about how instrument's are designed, you'd realize a company doesn't go back and redo an instrument. Now shut up and do us all a favor. Toni brings up a very good point.... you know it's pretty bad when a company is willing to pay your waste costs... Not a good business plan. Why not 'fix' the machine so that it's more "green" Maria Katleba MS HT(ASCP) ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From JWeems <@t> sjha.org Wed Sep 8 09:46:10 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Sep 8 09:46:17 2010 Subject: [Histonet] RE: Pathology billing for consultation In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403321E18E2@CHEXCMS10.one.ads.che.org> It is my understanding that we cannot charge professional fees for other pathologists. Our hospital pays for the pathologists' consults if the patient's insurance will not or if the consultant does not bill third party. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 09:22 To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ESabato <@t> stanfordmed.org Wed Sep 8 09:51:21 2010 From: ESabato <@t> stanfordmed.org (Sabato, Ellen) Date: Wed Sep 8 09:52:24 2010 Subject: [Histonet] unsubscribe Message-ID: Please unsubscribe. Ellen Sabato Histology Supervisor Stanford Hospital & Clinics Stanford, CA From alonso.martinezcanabal <@t> utoronto.ca Wed Sep 8 09:21:19 2010 From: alonso.martinezcanabal <@t> utoronto.ca (alonso.martinezcanabal@utoronto.ca) Date: Wed Sep 8 10:00:11 2010 Subject: [Histonet] About home made mounting medium Message-ID: <20100908102119.962t63mksg44sc4g@webmail.utoronto.ca> Hi everyone. I am trying to make aquous mouning medium for our lab. I am doing 10% PVV, 10% and 1% NPG in PBS. For a reason that I dont understand, when I try to mix the PVV wth the NPG the PVV starts to polymerize, even if it is completely dissolved. When I dissolve the NPG directly in the PVV solution it dissolves fine, but acquires a dark color that maybe be harmles but looks bad and apparently the appeareance is importnt in this lab... So, any suggestions? Thank you very much Alonso From ESabato <@t> stanfordmed.org Wed Sep 8 10:01:00 2010 From: ESabato <@t> stanfordmed.org (Sabato, Ellen) Date: Wed Sep 8 10:00:42 2010 Subject: [Histonet] Please unsubscribe Message-ID: Please unsubscribe Ellen Sabato Histology Supervisor Stanford Hospital & Clinics Stanford, CA From trathborne <@t> somerset-healthcare.com Wed Sep 8 10:05:08 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Sep 8 10:05:13 2010 Subject: [Histonet] Pathology billing for consultation In-Reply-To: Message-ID: If the request was made by the patient or their physician, then the patient is billed. They are informed of this by their physician after he/she contacts us. If it is requested by the pathologist because they are unsure of a diagnosis, then the pathologist will pay. I'm not referring to sending out the block or slide for testing that is not done at our facility, only cases where all relevant testing has been completed and the pathologist is still uncertain. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 9:22 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From talulahgosh <@t> gmail.com Wed Sep 8 10:19:54 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Sep 8 10:20:01 2010 Subject: [Histonet] unsubscribe In-Reply-To: References: Message-ID: Wait, try it again! It might work the third time!! -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Wed, Sep 8, 2010 at 10:51 AM, Sabato, Ellen wrote: > Please unsubscribe. > > > > Ellen Sabato > > Histology Supervisor > > Stanford Hospital & Clinics > > Stanford, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Janice.Mahoney <@t> alegent.org Wed Sep 8 10:25:51 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Sep 8 10:28:25 2010 Subject: [Histonet] RE: Ventana vs. Leica In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E6D@EXCHMBC2.ad.ah.local> In My opinion (maybe we all need to start prefacing our comments with this), all of the Ventana reps I have encountered are very professional and would NEVER write something lilke this about a customer. I'm sure if it happened he would be fired on the spot. Perfect time for one of my favorite quotes of all time "Life is very short and there's no time for fussing and fighting my friends." -Lennon&Mccartney Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 9:11 AM To: 'Maria Katleba'; histonet Subject: [Histonet] RE: Ventana vs. Leica There are many opinions on the histonet that I disagree with, however it is just an opinion and no one should be disrespected how joe jones decided to disrespect Maria. Lets all remember it is just an opinion and someone asked for it. Hopefully Joe Jones is not an employee of Ventana because it would definitely sway my opinion about who they hire and how the treat potential clients. Just my opinion. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Tuesday, September 07, 2010 6:31 PM To: histonet Subject: [Histonet] FW: Ventana vs. Leica This is how professional histotechs treat each other? A very sad commentary in a country where people are afforded free speech. Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime ________________________________ From: Joe Jones [mailto:joe_j_jones99@yahoo.com] Sent: Tuesday, September 07, 2010 4:28 PM To: Maria Katleba Subject: Ventana vs. Leica Maria, Please stop embarassing those of us who know you and have to work with you. You are clueless about Ventana's business, let alone anyone elses. Your postings on Histonet scream "idiot", so please stop. Ventana paying for a company's waste has nothing to do with a business plan, you dim ass. They pay it after some customer's bring up the additional cost when weighing their options. Fix the "machine" (it's not a machine you stupid tool)? If you knew anything about how instrument's are designed, you'd realize a company doesn't go back and redo an instrument. Now shut up and do us all a favor. Toni brings up a very good point.... you know it's pretty bad when a company is willing to pay your waste costs... Not a good business plan. Why not 'fix' the machine so that it's more "green" Maria Katleba MS HT(ASCP) ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Janice.Mahoney <@t> alegent.org Wed Sep 8 10:36:52 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Sep 8 10:37:03 2010 Subject: [Histonet] RE: Pathology billing for consultation In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16403321E18E2@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E16403321E18E2@CHEXCMS10.one.ads.che.org> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E6F@EXCHMBC2.ad.ah.local> If our Pathologists need a second opinion we eat the charge. If it is requested by the clinician, we request that he/she be billed. If it is the patient we bill the patient (or ask the consultant to bill the patient) All of this depends on patient status/medicare etc. I'm very interested in seeing how others respond. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, September 08, 2010 9:46 AM To: Demarinis, Carolyn; HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] RE: Pathology billing for consultation It is my understanding that we cannot charge professional fees for other pathologists. Our hospital pays for the pathologists' consults if the patient's insurance will not or if the consultant does not bill third party. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 09:22 To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Jessica.Vacca <@t> HCAhealthcare.com Wed Sep 8 10:45:11 2010 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Wed Sep 8 10:45:21 2010 Subject: [Histonet] RE: Pathology billing for consultation In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E6F@EXCHMBC2.ad.ah.local> References: <92AD9B20A6C38C4587A9FEBE3A30E16403321E18E2@CHEXCMS10.one.ads.che.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E6F@EXCHMBC2.ad.ah.local> Message-ID: <938D716CD445614ABBB817517557B6F4ED072DA5@NADCWPMSGCMS09.hca.corpad.net> We send to reference lab (with which we have a contract, if they do not have the pathologist that covers that specialty or need to then send it for additional consult outside of them, they will incur the cost and only charge for the initial consult) and it is always 3rd party billed, this will cover medicare and insurance. It's never billed back to us unless we mistakenly mark the req. incorrectly. Most of the larger reference labs take all insurances. Jessica Vacca Histology Supervisor Brandon Regional Hospital 813-571-6410 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, September 08, 2010 11:37 AM To: 'Weems, Joyce'; Demarinis, Carolyn; HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] RE: Pathology billing for consultation If our Pathologists need a second opinion we eat the charge. If it is requested by the clinician, we request that he/she be billed. If it is the patient we bill the patient (or ask the consultant to bill the patient) All of this depends on patient status/medicare etc. I'm very interested in seeing how others respond. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, September 08, 2010 9:46 AM To: Demarinis, Carolyn; HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] RE: Pathology billing for consultation It is my understanding that we cannot charge professional fees for other pathologists. Our hospital pays for the pathologists' consults if the patient's insurance will not or if the consultant does not bill third party. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 09:22 To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Wed Sep 8 10:51:14 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Sep 8 10:51:31 2010 Subject: [Histonet] RE: Pathology billing for consultation In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E6F@EXCHMBC2.ad.ah.local> References: <92AD9B20A6C38C4587A9FEBE3A30E16403321E18E2@CHEXCMS10.one.ads.che.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E6F@EXCHMBC2.ad.ah.local> Message-ID: That is the case here also -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, September 08, 2010 10:37 AM To: 'Weems, Joyce'; Demarinis, Carolyn; HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] RE: Pathology billing for consultation If our Pathologists need a second opinion we eat the charge. If it is requested by the clinician, we request that he/she be billed. If it is the patient we bill the patient (or ask the consultant to bill the patient) All of this depends on patient status/medicare etc. I'm very interested in seeing how others respond. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, September 08, 2010 9:46 AM To: Demarinis, Carolyn; HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] RE: Pathology billing for consultation It is my understanding that we cannot charge professional fees for other pathologists. Our hospital pays for the pathologists' consults if the patient's insurance will not or if the consultant does not bill third party. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 09:22 To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From vjp2105 <@t> columbia.edu Wed Sep 8 10:56:52 2010 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Wed Sep 8 10:56:57 2010 Subject: [Histonet] Laboratory move Message-ID: Hi everyone, I was wondering if anyone may have a laboratory relocation guidelines/checklist? Thanks so much From HornHV <@t> archildrens.org Wed Sep 8 11:23:44 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Sep 8 11:23:51 2010 Subject: [Histonet] RE: Pathology billing for consultation In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E16403321E18E2@CHEXCMS10.one.ads.che.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E6F@EXCHMBC2.ad.ah.local> Message-ID: <25A4DE08332B19499904459F00AAACB717F9A81E36@EVS1.archildrens.org> You cannot bill for consults if your pathologists requests them, is that correct? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 10:51 AM To: 'Mahoney,Janice A'; 'Weems, Joyce'; Demarinis, Carolyn; HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] RE: Pathology billing for consultation That is the case here also -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, September 08, 2010 10:37 AM To: 'Weems, Joyce'; Demarinis, Carolyn; HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] RE: Pathology billing for consultation If our Pathologists need a second opinion we eat the charge. If it is requested by the clinician, we request that he/she be billed. If it is the patient we bill the patient (or ask the consultant to bill the patient) All of this depends on patient status/medicare etc. I'm very interested in seeing how others respond. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, September 08, 2010 9:46 AM To: Demarinis, Carolyn; HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] RE: Pathology billing for consultation It is my understanding that we cannot charge professional fees for other pathologists. Our hospital pays for the pathologists' consults if the patient's insurance will not or if the consultant does not bill third party. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 09:22 To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Bill.Tench <@t> pph.org Wed Sep 8 11:48:25 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Sep 8 11:48:37 2010 Subject: [Histonet] billing consults Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A55C9@MAIL1.pph.local> we ask the consultant to bill the patient's insurance. If they don't do that, they bill the hospital and the hospital passes the charges on to the patient. we do not make any distinction based on where the request for the consultation came from (us, the patient, the treating clinician). The patient is the beneficiary of the service. On the very rare case when it clearly is an issue of intellectual curiosity (i can think of only 3 examples), the practice will pay the charge. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From gentras <@t> auburn.edu Wed Sep 8 12:01:51 2010 From: gentras <@t> auburn.edu (Atoska Gentry) Date: Wed Sep 8 12:02:17 2010 Subject: [Histonet] RE: Feline adipose tissue IHC Message-ID: <4C877B2F.C676.0026.0@auburn.edu> hello, I'm assisting a graduate student who's interested in info on source recommendations for the following macrophage markers which guarantee results on feline tissue: 1) AM3-K, 2) alpha1-antitrypsin and 3) Lysozyme. Your prompt replies with pertinent info will be greatly appreciated. ~ Atoska From ttruscot <@t> vetmed.wsu.edu Wed Sep 8 12:28:09 2010 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Sep 8 12:28:03 2010 Subject: [Histonet] RE: billing consults In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A55C9@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A55C9@MAIL1.pph.local> Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB40113757A74EF@CVMMBX.vetmed.wsu.edu> One way to think about it, would be that if a pathologist accepts a case, it is understood that he or she has the expertise to diagnose it, and with a diagnoses comes a bill. But, if unable to diagnose, or confidently diagnose, and a consult is needed, then the pathologist that can make the diagnosis gets paid, and the lab that did the preliminary work gets part of that pay. Would a mechanic charge you full price for fixing your engine, if he couldn't fix it and sent it to another mechanic to fix? Perhaps fee schedules should be set up so that a certain percentage goes to consultation ( because it's going to happen) and the patient doesn't get extra billing.Just an opinion. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, September 08, 2010 9:48 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] billing consults we ask the consultant to bill the patient's insurance. If they don't do that, they bill the hospital and the hospital passes the charges on to the patient. we do not make any distinction based on where the request for the consultation came from (us, the patient, the treating clinician). The patient is the beneficiary of the service. On the very rare case when it clearly is an issue of intellectual curiosity (i can think of only 3 examples), the practice will pay the charge. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Sep 8 12:38:49 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Sep 8 12:38:55 2010 Subject: [Histonet] unsubscribe In-Reply-To: References: Message-ID: <497F8C4844AA4BED1E6B1DB6@CDYwxp1931.ad.med.buffalo.edu> Personally and professionally, I think this response is uncalled for. I know I myself get many emails a day and I am tuned to look for the "To unsubscribe, please click here" link (or at least to look for the word UNSUBSCRIBE somewhere in the email). Seeing as how such a link is not overtly obvious on Histonet emails (the link is "diguised" as merely a list information link), I think I would be confused, too, if I ever wanted to unsubscribe from Histonet. It is unfortunate but true that in this fast-paced age of information inundation we need to have the obvious jumping out at us before we figure out what it is. Kind regards, Merced --On Wednesday, September 08, 2010 11:19 AM -0400 Emily Sours wrote: > Wait, try it again! It might work the third time!! > > -- > Outside of a dog, a book is man's best friend. Inside of a dog it's too > dark to read. > --Groucho Marx > > > On Wed, Sep 8, 2010 at 10:51 AM, Sabato, Ellen > wrote: > >> Please unsubscribe. >> >> >> >> Ellen Sabato >> >> Histology Supervisor >> >> Stanford Hospital & Clinics >> >> Stanford, CA >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From jfray80 <@t> hotmail.com Wed Sep 8 13:36:10 2010 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Wed Sep 8 13:55:09 2010 Subject: [Histonet] competency Message-ID: Would anyone like to share a copy of there competency form. I would like to make one up for the GI lab I am managing. Thanks Histojoe From jsantiago <@t> bellsouth.net Wed Sep 8 14:28:46 2010 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Wed Sep 8 14:28:50 2010 Subject: [Histonet] MHC Class 1 Message-ID: <681762.69328.qm@web180804.mail.gq1.yahoo.com> Histonetters, Anyone doing MHC Class I on paraffin embedded tissue? We have a case that we ned to send out. Please let me know if anyone is performing this test. Sincerely, Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville 655 W 8th Street Jacksonville, FL 32209 904-244-6149 jerry.santiago@jax.ufl.edu From HornHV <@t> archildrens.org Wed Sep 8 15:08:04 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Sep 8 15:08:08 2010 Subject: [Histonet] bar coding specimens, slides, blocks Message-ID: <25A4DE08332B19499904459F00AAACB717F9A81E3E@EVS1.archildrens.org> I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From gayle.callis <@t> bresnan.net Wed Sep 8 15:14:13 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Sep 8 15:14:25 2010 Subject: [Histonet] Will a Thermo Scientific Sales Representative please contact me Message-ID: <000001cb4f92$69e29e70$3da7db50$@callis@bresnan.net> I need to have a Thermo Scientific sales rep contact me to clarify ordering Richard Allan staining reagents. Thank you Gayle M. Callis HTL/HT/MT(ASCP) From HornHV <@t> archildrens.org Wed Sep 8 15:19:41 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Sep 8 15:19:48 2010 Subject: [Histonet] Will a Thermo Scientific Sales Representative please contact me In-Reply-To: <000001cb4f92$69e29e70$3da7db50$@callis@bresnan.net> References: <000001cb4f92$69e29e70$3da7db50$@callis@bresnan.net> Message-ID: <25A4DE08332B19499904459F00AAACB717F9A81E3F@EVS1.archildrens.org> Ditto...I need the same help Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Wednesday, September 08, 2010 3:14 PM To: 'Histonet' Subject: [Histonet] Will a Thermo Scientific Sales Representative please contact me I need to have a Thermo Scientific sales rep contact me to clarify ordering Richard Allan staining reagents. Thank you Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From lblazek <@t> digestivespecialists.com Wed Sep 8 15:40:22 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Sep 8 15:40:26 2010 Subject: [Histonet] Will a Thermo Scientific Sales Representative please contact me In-Reply-To: <25A4DE08332B19499904459F00AAACB717F9A81E3F@EVS1.archildrens.org> References: <000001cb4f92$69e29e70$3da7db50$@callis@bresnan.net> <25A4DE08332B19499904459F00AAACB717F9A81E3F@EVS1.archildrens.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C7E2@IBMB7Exchange.digestivespecialists.com> You mean there are ones? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:20 PM To: 'gayle callis'; 'Histonet' Subject: RE: [Histonet] Will a Thermo Scientific Sales Representative please contact me Ditto...I need the same help Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Wednesday, September 08, 2010 3:14 PM To: 'Histonet' Subject: [Histonet] Will a Thermo Scientific Sales Representative please contact me I need to have a Thermo Scientific sales rep contact me to clarify ordering Richard Allan staining reagents. Thank you Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Wed Sep 8 16:31:03 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Sep 8 16:31:10 2010 Subject: [Histonet] Odd question Message-ID: Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From sgoebel <@t> xbiotech.com Wed Sep 8 16:36:43 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Sep 8 16:36:50 2010 Subject: [Histonet] Odd question Message-ID: <20100908143643.9e2d9aa830e8449a2412eb1e4f2f067e.f92a086ba4.wbe@email04.secureserver.net> Formalin is a biohazard and I think there are restrictions on tra nsporting it anywhere (even home from the hospital). I know at past j It mention your faci that anything r facility? This usually Sarah Goebel, B.A., HT (ASCP) < Histotechn XBiotech USA Inc. 8201 East Riverside D Austin, Texas 78744 (51 -------- Original Message -------- Subject: [Histonet] Odd question From: [1]Kim.Donadio@bhcpns.org< 2:31 pm To: [2]histonet@lists.uts Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our < it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth [4]http: References 1. 3D"mailto:Kim.Donadio@bhcpns.org" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From nasteuer <@t> maxhealth.com Wed Sep 8 16:38:56 2010 From: nasteuer <@t> maxhealth.com (Nathan Steuer) Date: Wed Sep 8 16:39:25 2010 Subject: [Histonet] **Histo Technologist Contract in ATL** Message-ID: <6BA36D0AD1B7A4408D1B2E618BDADD8394147289C1@EXCLUSTER02.maxhealth.com> Hello, I have a 6 month Histology Technologist contract (local) in the city of Atlanta. Our client is looking for a registered technologist to come in and work from 7am-430pm, Monday through Friday. They are looking for a well rounded tech able to help in processing, embedding, sectioning and staining. They would like someone to be able to start as soon as possible. Realistically we are likely looking at a date between Sept 20th and Oct 4th. If you, or anyone you know, are interested in this opening give me a call or drop me an email at your convenience. Thank you, Nathan Steuer Maxim Staffing Solutions 2751 Buford Highway, Suite 202 Atlanta, GA 30324 PH: 404-325-3113 FX: 888-973-2726 ________________________________ Confidentiality Statement: The information contained in this facsimile/email transmission is privileged and confidential and is intended only for the use of the recipient listed above. If you are neither the intended recipient or an employee or agent of the intended recipient responsible for the delivery of this information, you are hereby notified that the disclosure, copying, use or distribution of this information is strictly prohibited. If you have received this transmission in error, please notify us immediately to arrange for the return of the transmitted documents or to verify their destruction. From CIngles <@t> uwhealth.org Wed Sep 8 16:39:50 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Sep 8 16:41:15 2010 Subject: [Histonet] Will a Thermo Scientific Sales Representative pleasecontact me References: <000001cb4f92$69e29e70$3da7db50$@callis@bresnan.net><25A4DE08332B19499904459F00AAACB717F9A81E3F@EVS1.archildrens.org> <5A2BD13465E061429D6455C8D6B40E390EAF72C7E2@IBMB7Exchange.digestivespecialists.com> Message-ID: Maybe they're all pestering me. :) I believe Leica has Thermo now. I know they have closed at least one factory in my region, maybe more. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Blazek, Linda Sent: Wed 9/8/2010 3:40 PM To: 'Horn, Hazel V'; 'gayle callis'; 'Histonet' Subject: RE: [Histonet] Will a Thermo Scientific Sales Representative pleasecontact me You mean there are ones? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:20 PM To: 'gayle callis'; 'Histonet' Subject: RE: [Histonet] Will a Thermo Scientific Sales Representative please contact me Ditto...I need the same help Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Wednesday, September 08, 2010 3:14 PM To: 'Histonet' Subject: [Histonet] Will a Thermo Scientific Sales Representative please contact me I need to have a Thermo Scientific sales rep contact me to clarify ordering Richard Allan staining reagents. Thank you Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Sep 8 16:43:51 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Sep 8 16:43:58 2010 Subject: [Histonet] Odd question In-Reply-To: References: Message-ID: <4C87CB57.7400.0077.1@harthosp.org> Here in CT we can only release tissue to a funeral home. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 9/8/2010 5:31 PM >>> Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Sep 8 16:45:37 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Sep 8 16:46:41 2010 Subject: [Histonet] Odd question In-Reply-To: <20100908143643.9e2d9aa830e8449a2412eb1e4f2f067e.f92a086ba4.wbe@email04.secureserver.net> References: <20100908143643.9e2d9aa830e8449a2412eb1e4f2f067e.f92a086ba4.wbe@email04.secureserver.net> Message-ID: <4C880401.5040201@pathology.washington.edu> After my surgery for a rare tumor, someone on Histonet was looking for that particular tumor for a control. They would not give me the wet tissue, even though I work here and had the surgery done here. They did process the remainder of the tissue into paraffin blocks which I then mailed off after signing a bunch of release forms. Just my personal experience. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 9/8/2010 2:36 PM, sgoebel@xbiotech.com wrote: > Formalin is a biohazard and I think there are restrictions on tra nsporting it anywhere (even home from the hospital). I know at past > j=obs, we did not allow anything that had touched formalin to leave. > It=ould be potential for lawsuits if somehow it got injested, not to > mention=if they saw something they didn't think was right? Doesn't > your faci=ity have something the patient signs before surgery saying > that anything r=emoved during surgery becomes property of the > facility? This usually =tops things quickly? > > Sarah Goebel, B.A., HT (ASCP) > > <=m> > > Histotechn=cian > > XBiotech USA Inc. > > 8201 East Riverside D=. Bldg 4 Suite 100 > Austin, Texas 78744 > (51=)386-5107 > > -------- Original Message -------- > Subject: [Histonet] Odd question > From: [1]Kim.Donadio@bhcpns.org<=br> Date: Wed, September 08, 2010 > 2:31 pm > To: [2]histonet@lists.uts=uthwestern.edu > Hi Histonetters, > Can anyone give me any idea of any laws that guide giving a patient > there organ after we have taken from it what we need to do the > Histology? > I know we have to keep it for a minimum of two weeks after sign out ( > our<=r> policy is 6 weeks after sign out ). But then we dispose of > it as medical waste. > Are any of you aware of any guidelines on giving a patient there > entire > organ which would be submerged in formalin? > Help and thanks in advance :-) > Kim Donadio > Pathology Supervisor > Baptist Hospital > 1000 W Moreno St. > Pensacola FL 32501 > Phone (850) 469-7718 > Fax (850) 434-4996 > ----------------------------------------- > All electronic data transmissions originating from or sent to > Baptist Health Care Corporation (BHC) are subject to monitoring. > This message along with any attached data, are the confidential and > proprietary communications of BHC and are intended to be received > only by the individual or individuals to whom the message has been > addressed. If the reader of this message is not the intended > recipient, please take notice that any use, copying, printing, > forwarding or distribution of this message, in any form, is > strictly prohibited and may violate State or Federal Law. If you > have received this transmission in error, please delete or destroy > all copies of this message. For questions, contact the BHC Privacy > Officer at (850) 434-4472. Rev.10/07. > _______________________________________________ > Histonet mailing list > [3]Histonet@lists.utsouth=estern.edu > [4]http:=/lists.utsouthwestern.edu/mailman/listinfo/histonet > > > References > > 1. 3D"mailto:Kim.Donadio@bhcpns.org" > 2. 3D"mailto:histonet@lists.utsouthwestern.edu" > 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Wed Sep 8 17:13:47 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Sep 8 17:14:07 2010 Subject: [Histonet] Odd question In-Reply-To: <20100908143643.9e2d9aa830e8449a2412eb1e4f2f067e.f92a086ba4.wbe@email04.secureserver.net> Message-ID: I felt the same way as you about the hazards. I hadn't thought about the what they signed before surgery. I will check into that. Thanks Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 09/08/2010 04:36 PM To Kim.Donadio@bhcpns.org cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] Odd question Formalin is a biohazard and I think there are restrictions on transporting it anywhere (even home from the hospital). I know at past jobs, we did not allow anything that had touched formalin to leave. It could be potential for lawsuits if somehow it got injested, not to mention if they saw something they didn't think was right? Doesn't your facility have something the patient signs before surgery saying that anything removed during surgery becomes property of the facility? This usually stops things quickly? Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] Odd question From: Kim.Donadio@bhcpns.org Date: Wed, September 08, 2010 2:31 pm To: histonet@lists.utsouthwestern.edu Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Wed Sep 8 17:14:03 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Sep 8 17:14:15 2010 Subject: [Histonet] returning tissue Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A55D2@MAIL1.pph.local> We do not return any tissue to patient unless there is a religious request that must be initiated by the patient (ie, staff are not permitted to offer this option--we consider that coaching). Then it is a big hassle with the issues of hazard exposure, disposal, etc. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From flnails <@t> texaschildrens.org Wed Sep 8 17:18:15 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Sep 8 17:18:32 2010 Subject: [Histonet] Odd question In-Reply-To: References: Message-ID: Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From Timothy.Morken <@t> ucsfmedctr.org Wed Sep 8 17:49:43 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Sep 8 17:49:54 2010 Subject: [Histonet] Odd question In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4C025501010F@EXMBMCB15.ucsfmedicalcenter.org> There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org). It also addresses wet tissue, other things taken out of people. The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials." So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 3:18 PM To: 'Kim.Donadio@bhcpns.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Odd question Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Sep 8 21:25:39 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Sep 8 21:26:05 2010 Subject: [Histonet] Will a Thermo Scientific Sales Representative pleasecontact me In-Reply-To: References: <000001cb4f92$69e29e70$3da7db50$@callis@bresnan.net><25A4DE08332B19499904459F00AAACB717F9A81E3F@EVS1.archildrens.org> <5A2BD13465E061429D6455C8D6B40E390EAF72C7E2@IBMB7Exchange.digestivespecialists.com> Message-ID: <83CC36CF-78AE-4E4E-9A5A-C786F7F99BF6@yahoo.com> Hi Claire, Sorry you are having difficulties with Thermo. Actually, Thermo is the parent of Fisher now, as well as Lab Vision and Richard Allen. Leica is not associated with Thermo what so ever. Leica is the parent company of "Vision Biosystems" now Leica Microsystems "Bond" and McCormick. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Sep 8, 2010, at 2:39 PM, Ingles Claire wrote: > Maybe they're all pestering me. :) I believe Leica has Thermo now. > I know they have closed at least one factory in my region, maybe more. > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Blazek, Linda > Sent: Wed 9/8/2010 3:40 PM > To: 'Horn, Hazel V'; 'gayle callis'; 'Histonet' > Subject: RE: [Histonet] Will a Thermo Scientific Sales > Representative pleasecontact me > > > > You mean there are ones? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Wednesday, September 08, 2010 4:20 PM > To: 'gayle callis'; 'Histonet' > Subject: RE: [Histonet] Will a Thermo Scientific Sales > Representative please contact me > > Ditto...I need the same help > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis > Sent: Wednesday, September 08, 2010 3:14 PM > To: 'Histonet' > Subject: [Histonet] Will a Thermo Scientific Sales Representative > please contact me > > I need to have a Thermo Scientific sales rep contact me to clarify > ordering > Richard Allan staining reagents. > > > > Thank you > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > ********************************************************************** > The information contained in this message may be privileged and > confidential > and protected from disclosure. If the reader of this message is not > the > intended recipient, or an employee or agent responsible for > delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is > strictly > prohibited. If you have received this communication in error, > please notify > us immediately by replying to the message and deleting it from your > computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From d.a.faichney <@t> stir.ac.uk Thu Sep 9 03:44:39 2010 From: d.a.faichney <@t> stir.ac.uk (Deborah Faichney) Date: Thu Sep 9 03:45:01 2010 Subject: [Histonet] H&E+ Alcian Blue Message-ID: <8ED3F2CA5B78E142B8193376C57330F8EAE96449C5@EXCH2007.ad.stir.ac.uk> Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. From TMcNemar <@t> lmhealth.org Thu Sep 9 05:04:06 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Sep 9 05:04:13 2010 Subject: [Histonet] RE: bar coding specimens, slides, blocks In-Reply-To: <25A4DE08332B19499904459F00AAACB717F9A81E3E@EVS1.archildrens.org> Message-ID: Also interested in this... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Timothy.Morken <@t> ucsfmedctr.org Thu Sep 9 10:48:05 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Sep 9 10:48:16 2010 Subject: FW: [Histonet] Odd question Message-ID: <1AAF670737F193429070841C6B2ADD4C0255010115@EXMBMCB15.ucsfmedicalcenter.org> This link will take you to the article about legalisms of tissue and blocks: http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12 Tim >>> "Morken, Tim" 9/8/2010 6:49 PM >>> There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org). It also addresses wet tissue, other things taken out of people. The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials." So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 3:18 PM To: 'Kim.Donadio@bhcpns.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Odd question Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Thu Sep 9 11:03:55 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Sep 9 11:04:01 2010 Subject: [Histonet] bar coding specimens, slides, blocks In-Reply-To: <25A4DE08332B19499904459F00AAACB717F9A81E3E@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB717F9A81E3E@EVS1.archildrens.org> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F461@exchange.cmc-nh.org> We have had a great deal of success using Leica's IPC and IPS cassette and slide labelers. We use Soft Path but I had heard that the Leica LIS experts have experience interfacing with a number of LIS systems. We set these up basically as just another printer that is interfaced with our LIS system. The software within the Leica labelers produces the cassettes and slides with any configuration of accession number and/or bar code that you would like. We chose to use 2d barcode to save space on the cassette and slide. We also print our ThinPrep slides this way. Leica and Hologic got together to make sure we were able to use the correct coding to use these slides on our ThinPrep Imager. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Thu Sep 9 11:06:21 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Sep 9 11:06:26 2010 Subject: [Histonet] Pathology billing for consultation In-Reply-To: References: Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F462@exchange.cmc-nh.org> There are a couple of ways to handle this and most of them have already been mentioned. We have a contract Pathology group in our hospital so if the patient insurance does not pay, the consultant bills the Pathology Group. Unless your pathologists are completely sold on their expert consultants, outside labs such as Genzyme will do the third party billing if you use their consultants. I know that they accept insurance assignment and have relationships with most large insurers so the patient rarely gets stuck with a bill. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 9:22 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Thu Sep 9 11:12:55 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Thu Sep 9 11:13:07 2010 Subject: FW: [Histonet] Odd question In-Reply-To: <1AAF670737F193429070841C6B2ADD4C0255010115@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: Thank you all for responding. All of you were very helpful. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Morken, Tim" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2010 10:48 AM To "histonet@lists.utsouthwestern.edu" cc Subject FW: [Histonet] Odd question This link will take you to the article about legalisms of tissue and blocks: http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12 Tim >>> "Morken, Tim" 9/8/2010 6:49 PM >>> There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org). It also addresses wet tissue, other things taken out of people. The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials." So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 3:18 PM To: 'Kim.Donadio@bhcpns.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Odd question Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Thu Sep 9 11:14:12 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Thu Sep 9 11:14:24 2010 Subject: [Histonet] giving back tissue Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A55D6@MAIL1.pph.local> In all of this discussion, it is important to understand that there are significant variations in state laws that relate to this issue, so if this problem arises, have your hospital/lab attorney check into state laws very carefully. In California, the laboratory may not "own" the tissue (again, depending on releases never read but signed by the patient on admission) but it clearly is the responsible "custodian." This applies to sending out slides and blocks for research and for clinical testing. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From STACEY.LANGENBERG <@t> UCDENVER.EDU Thu Sep 9 11:16:11 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Thu Sep 9 11:16:23 2010 Subject: [Histonet] bar coding specimens, slides, blocks In-Reply-To: <73A7ED895EE0C24D9267ED814911DF191773F461@exchange.cmc-nh.org> References: <25A4DE08332B19499904459F00AAACB717F9A81E3E@EVS1.archildrens.org><73A7ED895EE0C24D9267ED814911DF191773F461@exchange.cmc-nh.org> Message-ID: <99316908.1375278.1284048976798.JavaMail.rim@bda2340.bisx.prod.on.blackberry> We too use both the IPS and IPC. These are on such an open system we created our own LIS and now have barcoding from grossing to slide signout. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: "Feher, Stephen" Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Thu, 9 Sep 2010 10:03:55 To: Horn, Hazel V; HISTONET@PATHOLOGY.SWMED.EDU Subject: RE: [Histonet] bar coding specimens, slides, blocks We have had a great deal of success using Leica's IPC and IPS cassette and slide labelers. We use Soft Path but I had heard that the Leica LIS experts have experience interfacing with a number of LIS systems. We set these up basically as just another printer that is interfaced with our LIS system. The software within the Leica labelers produces the cassettes and slides with any configuration of accession number and/or bar code that you would like. We chose to use 2d barcode to save space on the cassette and slide. We also print our ThinPrep slides this way. Leica and Hologic got together to make sure we were able to use the correct coding to use these slides on our ThinPrep Imager. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Thu Sep 9 11:21:24 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Thu Sep 9 11:21:31 2010 Subject: [Histonet] HT position in Colorado Message-ID: <6F33D8418806044682A391273399860F051F3FA5@s-irv-ex301.PathologyPartners.intranet> Colorado GI Pathology (CGIP) is seeking an experienced histotech to complement its existing staff. CGIP is a specialized lab located in Centennial, CO that provides services to the three largest gastroenterology practices in Denver. Dr. Russell Nash is the chief pathologist and medical director. Tracy Wrinkle is the Operations Manager. The position can be filled by an experienced person who can work from 32 to 40 hours per week. The schedule is basically days, although coverage for other times during vacations will be needed. The pay range starts at $26.00 per hour with benefits. This is a small, congenial work place with an emphasis on team work and cooperation. If you are interested in learning more about the opportunity, please contact Tracy Wrinkle at twrinkle@cogipath.com or call her at (303) 770-4848 for a confidential interview and tour of the facility. Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From benoit.loup <@t> jouy.inra.fr Thu Sep 9 11:23:04 2010 From: benoit.loup <@t> jouy.inra.fr (Benoit Loup) Date: Thu Sep 9 11:23:08 2010 Subject: [Histonet] tissue refixation ? Message-ID: <4C8909E8.7040800@jouy.inra.fr> Hi to all, I have some troubles with H/E staining of tissues fixed in Bouin's. In fact it worked very well during my first assays and now my tissue sections are torn and looks like bad. I think that the fixation time was not sufficient (4h for 3mmx2mmx2mm pieces). Thus is it possible to dewax, rehydrate and refix tissue in bouin's before sectioning ? I also have some pieces already sectioned and mounted on slide but not yet dewaxed and stained. Is it also possible to refix these sections or are they definitively lost ? Thanks to all for your comments and help. Benoit -- Benoit Loup, PhD UMR Biologie du D?veloppement et Reproduction Diff?renciation des Gonades et Perturbations INRA ? Domaine de Vilvert B?timent Jacques Poly 78350 Jouy en Josas France Tel: 33 1 34 65 25 38 Fax: 33 1 34 65 22 41 E-mail: benoit.loup@jouy.inra.fr From llewllew <@t> shaw.ca Thu Sep 9 11:43:03 2010 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Sep 9 11:43:08 2010 Subject: [Histonet] H&E+ Alcian Blue In-Reply-To: <8ED3F2CA5B78E142B8193376C57330F8EAE96449C5@EXCH2007.ad.stir.ac.uk> References: <8ED3F2CA5B78E142B8193376C57330F8EAE96449C5@EXCH2007.ad.stir.ac.uk> Message-ID: You are probably using a regressive hematoxylin. Most of those stain acid mucins purple-blue. To overcome it use a strictly progressive hematoxylin such as Mayer (hx 1 g., alum 50 g.) for 5 minutes. The order should be alcian blue, wash, H&E. Bryan Llewellyn ----- Original Message ----- From: "Deborah Faichney" To: Sent: Thursday, September 09, 2010 1:44 AM Subject: [Histonet] H&E+ Alcian Blue Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maggie.allen <@t> nicewareintl.com Thu Sep 9 12:31:03 2010 From: maggie.allen <@t> nicewareintl.com (Maggie Allen) Date: Thu Sep 9 12:31:12 2010 Subject: [Histonet] RE: Histonet Digest, Vol 82, Issue 11 In-Reply-To: <20100909170227.D3ED4E8162@spamfilter2.redanvil.net> References: <20100909170227.D3ED4E8162@spamfilter2.redanvil.net> Message-ID: Our software (NiceLabel / NiceWatch) has the ability to take in data from any system. Data can be text, XML, HL7, JOB files, open formatted files, etc. We can connect via TCP/IP, Web Services, COM Port or a file drop. The data is them parsed out and place onto a label template formatted in NiceLabel Pro. A GUI design tool. We support all barcode symbologies (1D and 2D). We can then output to any printer that has a Windows print driver. Thermal, Laser, Line Matrix, Ink Jet or devices like slide and cassette printers. Please contact me if you would like additional information, a demo or trial of our software. Thank you, Maggie Allen Healthcare Business Development Manager Niceware International, LLC Tel (810) 629-3930 Cell (215) 200-0268 Email: maggie.allen@nicewareintl.com www.nicewareintl.com http://healthcare.nicewareintl.com Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 Corporate Numbers : General: (262) 784-2456 Toll Free: (888) 894-NICE (6423) Fax: (262) 784-2495 Technical Support: (262) 784-2466 Email: maggie.allen@nicewareintl.com www.nicewareintl.com http://healthcare.nicewareintl.com FREE Webinars : September 17, 2010 - 10:30AM CST - 11:30AM CST Learn How to Recognize NiceForm Application Opportunities Register Today! September 24, 2010 - 10:30AM CST - 11:30AM CST High Quality Color Labeling with NiceLabel Register Today! "The information in this e-mail and any attachments is confidential and may be subject to legal professional privilege. It is intended solely for the attention and use of the named addressee(s). If you are not the intended recipient or person responsible for delivering this information to the intended recipient, please notify the sender immediately. Unless you are the intended recipient or his/her representative you are not authorized to, and must not, read, copy, distribute, use or retain this message or any part of it" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, September 09, 2010 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 82, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. H&E+ Alcian Blue (Deborah Faichney) 2. RE: bar coding specimens, slides, blocks (Tom McNemar) 3. FW: [Histonet] Odd question (Morken, Tim) 4. RE: bar coding specimens, slides, blocks (Feher, Stephen) 5. RE: Pathology billing for consultation (Feher, Stephen) 6. Re: FW: [Histonet] Odd question (Kim.Donadio@bhcpns.org) 7. giving back tissue (Tench, Bill) 8. Re: bar coding specimens, slides, blocks (Langenberg, Stacey) 9. HT position in Colorado (Hale, Meredith) 10. tissue refixation ? (Benoit Loup) 11. Re: H&E+ Alcian Blue (Bryan Llewellyn) ---------------------------------------------------------------------- Message: 1 Date: Thu, 9 Sep 2010 09:44:39 +0100 From: Deborah Faichney Subject: [Histonet] H&E+ Alcian Blue To: "histonet@lists.utsouthwestern.edu" Message-ID: <8ED3F2CA5B78E142B8193376C57330F8EAE96449C5@EXCH2007.ad.stir.ac.uk> Content-Type: text/plain; charset="us-ascii" Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. ------------------------------ Message: 2 Date: Thu, 9 Sep 2010 06:04:06 -0400 From: Tom McNemar Subject: [Histonet] RE: bar coding specimens, slides, blocks To: "Horn, Hazel V" , "HISTONET@PATHOLOGY.SWMED.EDU" Message-ID: Content-Type: text/plain; charset="us-ascii" Also interested in this... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 3 Date: Thu, 9 Sep 2010 08:48:05 -0700 From: "Morken, Tim" Subject: FW: [Histonet] Odd question To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4C0255010115@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii This link will take you to the article about legalisms of tissue and blocks: http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12 Tim >>> "Morken, Tim" 9/8/2010 6:49 PM >>> There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org). It also addresses wet tissue, other things taken out of people. The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials." So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 3:18 PM To: 'Kim.Donadio@bhcpns.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Odd question Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 9 Sep 2010 12:03:55 -0400 From: "Feher, Stephen" Subject: RE: [Histonet] bar coding specimens, slides, blocks To: "Horn, Hazel V" , Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F461@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" We have had a great deal of success using Leica's IPC and IPS cassette and slide labelers. We use Soft Path but I had heard that the Leica LIS experts have experience interfacing with a number of LIS systems. We set these up basically as just another printer that is interfaced with our LIS system. The software within the Leica labelers produces the cassettes and slides with any configuration of accession number and/or bar code that you would like. We chose to use 2d barcode to save space on the cassette and slide. We also print our ThinPrep slides this way. Leica and Hologic got together to make sure we were able to use the correct coding to use these slides on our ThinPrep Imager. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 9 Sep 2010 12:06:21 -0400 From: "Feher, Stephen" Subject: RE: [Histonet] Pathology billing for consultation To: "Demarinis, Carolyn" , Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F462@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" There are a couple of ways to handle this and most of them have already been mentioned. We have a contract Pathology group in our hospital so if the patient insurance does not pay, the consultant bills the Pathology Group. Unless your pathologists are completely sold on their expert consultants, outside labs such as Genzyme will do the third party billing if you use their consultants. I know that they accept insurance assignment and have relationships with most large insurers so the patient rarely gets stuck with a bill. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 9:22 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 9 Sep 2010 11:12:55 -0500 From: Kim.Donadio@bhcpns.org Subject: Re: FW: [Histonet] Odd question To: "Morken, Tim" Cc: "histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Thank you all for responding. All of you were very helpful. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Morken, Tim" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2010 10:48 AM To "histonet@lists.utsouthwestern.edu" cc Subject FW: [Histonet] Odd question This link will take you to the article about legalisms of tissue and blocks: http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12 Tim >>> "Morken, Tim" 9/8/2010 6:49 PM >>> There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org). It also addresses wet tissue, other things taken out of people. The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials." So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 3:18 PM To: 'Kim.Donadio@bhcpns.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Odd question Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 9 Sep 2010 09:14:12 -0700 From: "Tench, Bill" Subject: [Histonet] giving back tissue To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A55D6@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii In all of this discussion, it is important to understand that there are significant variations in state laws that relate to this issue, so if this problem arises, have your hospital/lab attorney check into state laws very carefully. In California, the laboratory may not "own" the tissue (again, depending on releases never read but signed by the patient on admission) but it clearly is the responsible "custodian." This applies to sending out slides and blocks for research and for clinical testing. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 8 Date: Thu, 9 Sep 2010 10:16:11 -0600 From: "Langenberg, Stacey" Subject: Re: [Histonet] bar coding specimens, slides, blocks To: "Feher, Stephen" , "histonet-bounces@lists.utsouthwestern.edu" , "Horn, Hazel V" , "HISTONET@PATHOLOGY.SWMED.EDU" Message-ID: <99316908.1375278.1284048976798.JavaMail.rim@bda2340.bisx.prod.on.blackberry> Content-Type: text/plain; charset="iso-8859-1" We too use both the IPS and IPC. These are on such an open system we created our own LIS and now have barcoding from grossing to slide signout. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: "Feher, Stephen" Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Thu, 9 Sep 2010 10:03:55 To: Horn, Hazel V; HISTONET@PATHOLOGY.SWMED.EDU Subject: RE: [Histonet] bar coding specimens, slides, blocks We have had a great deal of success using Leica's IPC and IPS cassette and slide labelers. We use Soft Path but I had heard that the Leica LIS experts have experience interfacing with a number of LIS systems. We set these up basically as just another printer that is interfaced with our LIS system. The software within the Leica labelers produces the cassettes and slides with any configuration of accession number and/or bar code that you would like. We chose to use 2d barcode to save space on the cassette and slide. We also print our ThinPrep slides this way. Leica and Hologic got together to make sure we were able to use the correct coding to use these slides on our ThinPrep Imager. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 9 Sep 2010 11:21:24 -0500 From: "Hale, Meredith" Subject: [Histonet] HT position in Colorado To: Cc: "Roupp, Kevin" Message-ID: <6F33D8418806044682A391273399860F051F3FA5@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Colorado GI Pathology (CGIP) is seeking an experienced histotech to complement its existing staff. CGIP is a specialized lab located in Centennial, CO that provides services to the three largest gastroenterology practices in Denver. Dr. Russell Nash is the chief pathologist and medical director. Tracy Wrinkle is the Operations Manager. The position can be filled by an experienced person who can work from 32 to 40 hours per week. The schedule is basically days, although coverage for other times during vacations will be needed. The pay range starts at $26.00 per hour with benefits. This is a small, congenial work place with an emphasis on team work and cooperation. If you are interested in learning more about the opportunity, please contact Tracy Wrinkle at twrinkle@cogipath.com or call her at (303) 770-4848 for a confidential interview and tour of the facility. Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com ------------------------------ Message: 10 Date: Thu, 09 Sep 2010 18:23:04 +0200 From: Benoit Loup Subject: [Histonet] tissue refixation ? To: histonet@lists.utsouthwestern.edu Message-ID: <4C8909E8.7040800@jouy.inra.fr> Content-Type: text/plain; charset=windows-1252; format=flowed Hi to all, I have some troubles with H/E staining of tissues fixed in Bouin's. In fact it worked very well during my first assays and now my tissue sections are torn and looks like bad. I think that the fixation time was not sufficient (4h for 3mmx2mmx2mm pieces). Thus is it possible to dewax, rehydrate and refix tissue in bouin's before sectioning ? I also have some pieces already sectioned and mounted on slide but not yet dewaxed and stained. Is it also possible to refix these sections or are they definitively lost ? Thanks to all for your comments and help. Benoit -- Benoit Loup, PhD UMR Biologie du D?veloppement et Reproduction Diff?renciation des Gonades et Perturbations INRA ? Domaine de Vilvert B?timent Jacques Poly 78350 Jouy en Josas France Tel: 33 1 34 65 25 38 Fax: 33 1 34 65 22 41 E-mail: benoit.loup@jouy.inra.fr ------------------------------ Message: 11 Date: Thu, 9 Sep 2010 09:43:03 -0700 From: "Bryan Llewellyn" Subject: Re: [Histonet] H&E+ Alcian Blue To: "Histonet" Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original You are probably using a regressive hematoxylin. Most of those stain acid mucins purple-blue. To overcome it use a strictly progressive hematoxylin such as Mayer (hx 1 g., alum 50 g.) for 5 minutes. The order should be alcian blue, wash, H&E. Bryan Llewellyn ----- Original Message ----- From: "Deborah Faichney" To: Sent: Thursday, September 09, 2010 1:44 AM Subject: [Histonet] H&E+ Alcian Blue Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 82, Issue 11 **************************************** From txbobcat00 <@t> gmail.com Thu Sep 9 12:54:10 2010 From: txbobcat00 <@t> gmail.com (Em) Date: Thu Sep 9 12:54:13 2010 Subject: [Histonet] Melanoma Markers Message-ID: Hello, Does anyone have experience working up the following antibodys on a Ventana automated platform and would be able to offer a protocol recommendation? KAT13B from Abcam or Wnt2 from BioVision? Thank you! From tanisha.neely <@t> covance.com Thu Sep 9 14:08:44 2010 From: tanisha.neely <@t> covance.com (Neely, Tanisha) Date: Thu Sep 9 14:08:59 2010 Subject: [Histonet] IMAGES NEEDED - Special Stains/Carbohydrates Message-ID: <816E3C72F855F14985FC31D7C963AE6F21DD28CB@indexch03.ent.covance.com> Hello Histonetters: I am in need of some microscopic images of special stains that can be used in a continuing education course I am developing. While there are many images on the internet, I must have copyright permission to use them. In particular, I need images of the following stains: PAS PAS with Diastase Mucicarmine Alcian Blue Colloidal Iron If you own any of these images and are willing to allow me to use them in the course, please contact me at tanisha.neely@covance.com Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From kacausem <@t> maxhealth.com Thu Sep 9 14:14:20 2010 From: kacausem <@t> maxhealth.com (Kala Causemaker) Date: Thu Sep 9 14:14:27 2010 Subject: [Histonet] Full time histo tech wanted Message-ID: <646A6ADE598DD243B697C1A1B468949C0C5BE9C7A5@EXCLUSTER02.maxhealth.com> Good Afternoon, My name is Kala Causemaker and I am a healthcare recruiter with Maxim Staffing Solutions. At this time, we have a full-time opening in histology that we are recruiting for in the Chicagoland area. At least one year of experience in a general histology department is required. Experience with Tissue-Tek equipment is a plus. The candidate will be responsible for embedding, cutting, staining, and cover slipping H&E slides. All special stains, IHC, cytology, and grossing assistance will be handled by other staff. The position is set to start on September 20th. If you are interested please contact Kala or Justin at 708-358-9100, toll free at 866-541-1234, or simply respond to this email. We look forward to hearing from you! Kala J. Causemaker Healthcare Recruiter Maxim Staffing Solutions 1011 Lake St., Ste 308 Oak Park, IL 60301 P:708.358.9100 F:877.306.6797 kacausem@maxhealth.com ________________________________ Confidentiality Statement: The information contained in this facsimile/email transmission is privileged and confidential and is intended only for the use of the recipient listed above. If you are neither the intended recipient or an employee or agent of the intended recipient responsible for the delivery of this information, you are hereby notified that the disclosure, copying, use or distribution of this information is strictly prohibited. If you have received this transmission in error, please notify us immediately to arrange for the return of the transmitted documents or to verify their destruction. From dholmes <@t> umc.edu Thu Sep 9 14:38:18 2010 From: dholmes <@t> umc.edu (Dianne E. Holmes) Date: Thu Sep 9 14:38:32 2010 Subject: [Histonet] decal arteries? Message-ID: Has anyone got a quick and easy protocol for removing plaque from an artery without damaging the walls of the vessel. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From Bill.Tench <@t> pph.org Thu Sep 9 14:42:44 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Thu Sep 9 14:42:55 2010 Subject: [Histonet] decal arteries Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A55DE@MAIL1.pph.local> We routinely decal these. Most often for part of a day, but if really dense, overnight. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From Laura.Miller <@t> leica-microsystems.com Thu Sep 9 16:02:01 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Thu Sep 9 16:02:09 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 09/09/2010 and will not return until 09/13/2010. I am on vaction. I will return to the office on Monday, September 13, 2010. I will respond to your message when I return. If you need immediate assistance, please email David Archambault. Thank you. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From macveigh <@t> usc.edu Thu Sep 9 16:47:29 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Thu Sep 9 16:47:21 2010 Subject: [Histonet] Histology stainers Message-ID: <356BA88EA30F44269DE017A15DB48CC3@DFS66DD1> Hi histoneters, We have an old Jung Autostainer XL, which has been great, but it is very old and on its way out. What kind of newer stainers are you guys using? Should I even look at something diferent? I also would like to hear from the people using Exelsior tissue processor. Do you guys like it and how does it compare to the good old VIP? Michelle Aloni MS HTL ASCP Research Specialist USC Keck School of Medicine LA, CA From AnthonyH <@t> chw.edu.au Thu Sep 9 18:32:38 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Sep 9 18:32:52 2010 Subject: [Histonet] H&E+ Alcian Blue In-Reply-To: <8ED3F2CA5B78E142B8193376C57330F8EAE96449C5@EXCH2007.ad.stir.ac.uk> Message-ID: Debbie, It seems that the eosin is masking the alcian blue staining (ie changing the colour of the alcian blue). Try differentiating (or removing) the eosin by prolonged washing in water after the eosin stain. Beware this might occur quite quickly so monitor it microscopically. If you inadverrtantly over-differentiate, then just restain in the eosin and differentiate again. The eosin counterstain should not be too strong (not as vivid as your normal H&E). It is also advisable to choose a puple Hx (like Harrises) rather than a blue HX (like Mayers). I am not sure what Haematoxylin Z is. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deborah Faichney Sent: Thursday, 9 September 2010 6:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E+ Alcian Blue Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Janice.Mahoney <@t> alegent.org Fri Sep 10 07:38:56 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Sep 10 07:39:04 2010 Subject: [Histonet] LIS, HIS question Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E79@EXCHMBC2.ad.ah.local> Good Morning Everyone, Happy Friday, I'm interested to know if anyone is paperless with Histology ordering from surgery. We still use requisitions and are beginning the quest to go paperless but are having difficulty with all the variables we have in regard to the specimens we receive. Jan Mahoney Omaha, NE GO HUSKERS! ________________________________ Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From sraibley <@t> yahoo.com Fri Sep 10 09:51:43 2010 From: sraibley <@t> yahoo.com (Susan Raibley) Date: Fri Sep 10 09:51:47 2010 Subject: [Histonet] Flat Ice??? Message-ID: <106514.19159.qm@web56003.mail.re3.yahoo.com> Hello! I would greatly appreciate any tips or tricks to getting ice to freeze flat in the pan! We are tired of opening the freezer to see mountains that have formed in our pans overnight, making it hard to try and fit all the blocks we need to microtome on them. We have two freezers and they both have this problem. Help please! Thanks! ? ? Susan Bincsik, HT (ASCP) ? From MSHERWOOD <@t> PARTNERS.ORG Fri Sep 10 09:58:01 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Sep 10 09:58:06 2010 Subject: [Histonet] Flat Ice??? In-Reply-To: <106514.19159.qm@web56003.mail.re3.yahoo.com> References: <106514.19159.qm@web56003.mail.re3.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24609@PHSXMB30.partners.org> We use a styrofoam container and have no problem with it freezing flat. Are you containers sitting flat in the freezer? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Raibley Sent: Friday, September 10, 2010 10:52 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Flat Ice??? Hello! I would greatly appreciate any tips or tricks to getting ice to freeze flat in the pan! We are tired of opening the freezer to see mountains that have formed in our pans overnight, making it hard to try and fit all the blocks we need to microtome on them. We have two freezers and they both have this problem. Help please! Thanks! ? ? Susan Bincsik, HT (ASCP) ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From sfeher <@t> CMC-NH.ORG Fri Sep 10 10:24:51 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Sep 10 10:24:56 2010 Subject: [Histonet] LIS, HIS question In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E79@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E79@EXCHMBC2.ad.ah.local> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F47B@exchange.cmc-nh.org> What LIS and HIS systems are you using Jan? We are beginning our quest to do electronic order entry between SoftPath and our Sunrise HIS system. I know that Dartmouth Hitchcock Med Ctr in NH was successful in doing this from Cerner Millennium as well. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Friday, September 10, 2010 8:39 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] LIS, HIS question Good Morning Everyone, Happy Friday, I'm interested to know if anyone is paperless with Histology ordering from surgery. We still use requisitions and are beginning the quest to go paperless but are having difficulty with all the variables we have in regard to the specimens we receive. Jan Mahoney Omaha, NE GO HUSKERS! ________________________________ Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robinsoc <@t> mercyhealth.com Fri Sep 10 10:50:17 2010 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Fri Sep 10 10:50:55 2010 Subject: [Histonet] LIS, HIS question In-Reply-To: <73A7ED895EE0C24D9267ED814911DF191773F47B@exchange.cmc-nh.org> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E79@EXCHMBC2.ad.ah.local> <73A7ED895EE0C24D9267ED814911DF191773F47B@exchange.cmc-nh.org> Message-ID: <4C8A0D69.59AC.00AF.0@mercyhealth.com> We have Cerner Millennium and have been successful having nursing order in PowerChart and the order comes over into Pathnet. Some of the information required doesn't transmit currently. However, it is available to the path in PowerChart. You can contact me off line if you like, Jan. Also, I will be at the NHS meeting and I heard you are going as well. If so I could bring some examples of specimen labels and how we built the generic order in the Clin Lab system for tissues, fluids, pap, and bone marrows and we could have dinner together. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> "Feher, Stephen" 09/10/2010 10:24 AM >>> What LIS and HIS systems are you using Jan? We are beginning our quest to do electronic order entry between SoftPath and our Sunrise HIS system. I know that Dartmouth Hitchcock Med Ctr in NH was successful in doing this from Cerner Millennium as well. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Friday, September 10, 2010 8:39 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] LIS, HIS question Good Morning Everyone, Happy Friday, I'm interested to know if anyone is paperless with Histology ordering from surgery. We still use requisitions and are beginning the quest to go paperless but are having difficulty with all the variables we have in regard to the specimens we receive. Jan Mahoney Omaha, NE GO HUSKERS! ________________________________ Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Fri Sep 10 11:03:16 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Sep 10 11:03:25 2010 Subject: [Histonet] Flat Ice??? In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24609@PHSXMB30.partners.org> References: <106514.19159.qm@web56003.mail.re3.yahoo.com>, <073AE2BEA1C2BA4A8837AB6C4B943D9703E24609@PHSXMB30.partners.org> Message-ID: For quality assurance and safety concerns, I would suggest moving away from the "wet ice" and go with a sealed freezing tray. We moved to the small Histo-Cool ice trays w/ tray. http://www.labstore.com/catalog/index.cfm?Category_ID=375 William DeSalvo, B.S., HTL(ASCP) > Date: Fri, 10 Sep 2010 10:58:01 -0400 > From: MSHERWOOD@PARTNERS.ORG > To: sraibley@yahoo.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Flat Ice??? > CC: > > We use a styrofoam container and have no problem with it freezing flat. Are you > containers sitting flat in the freezer? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Raibley > Sent: Friday, September 10, 2010 10:52 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Flat Ice??? > > Hello! I would greatly appreciate any tips or tricks to getting ice to freeze > flat in the pan! We are tired of opening the freezer to see mountains that have > formed in our pans overnight, making it hard to try and fit all the blocks we > need to microtome on them. We have two freezers and they both have this problem. > Help please! Thanks! > > > Susan Bincsik, HT (ASCP) > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Fri Sep 10 12:46:11 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Fri Sep 10 12:46:32 2010 Subject: [Histonet] H&E+ Alcian Blue In-Reply-To: <8ED3F2CA5B78E142B8193376C57330F8EAE96449C5@EXCH2007.ad.stir.ac.uk> Message-ID: It wont remain turquoise as long as you use the eosin. At least this is my experience. I have known some Pathologist to read the mucin that way (purple). Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Deborah Faichney Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2010 03:44 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] H&E+ Alcian Blue Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From tchilton <@t> kumc.edu Fri Sep 10 13:13:13 2010 From: tchilton <@t> kumc.edu (Timothy Chilton) Date: Fri Sep 10 13:13:39 2010 Subject: [Histonet] solvent recycling Message-ID: <4C8A2EE9.002D.0049.1@kumc.edu> I am researching information on solvent recovery systems and could use some help specifically on B/R Instruments. We currently use the CBG Biotech recycler for alcohol use only but have been given funds from our environment of safety department to purchase two solvent recyclers to be used for alcohol, xylene, and formalin. If anyone is using the B/R 9700 ProCycler Advantage to regulate all three waste solvents in one system I would greatly appreciate any feedback. Thanks in advance, Timothy Chilton, HT (ASCP) Histology Supervisor University of Kansas Hospital 3901 Rainbow Blvd Kansas City, Kansas 66160 phone: (913) 588-1134 fax: (913) 588-8780 tchilton@kumc.edu From Herrick.James <@t> mayo.edu Fri Sep 10 14:33:58 2010 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Fri Sep 10 14:34:05 2010 Subject: [Histonet] Liver Processing Question Message-ID: <7267A64D75F58241B577876D8A885631038FE2AB@msgebe41> Hello everyone, I am trying to process and embed liver tissue in MMA. I used the same protocol that has worked very well a hundred times over, for harder tissues, but when used on the liver tissue, polymerized into a relatively soft and rubbery textured plastic for one of the two specimens (the second of the two also being too soft to properly section). If anyone would have any ideas/suggestions or protocols for me to follow, it would be very much appreciated. Thanks and have a great weekend!! Jim From wdesalvo.cac <@t> hotmail.com Fri Sep 10 14:51:21 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Sep 10 14:51:26 2010 Subject: [Histonet] solvent recycling In-Reply-To: <4C8A2EE9.002D.0049.1@kumc.edu> References: <4C8A2EE9.002D.0049.1@kumc.edu> Message-ID: I suggest you look into Creative Waste Solutions Inc. for recycling (actually filtering through a slurry) your Formalin and Alcohols. I have over 8 years experience with the filtering systems. Dirty solution in, clean solution out and there is no breakdown of the chemical, just a cleaning (crystal clear and pure). For alcohol we combine several grades and then re-use them in all dilutions. Fresh alcohol is used for "100%" steps. For the Formalin, we combine filtered, test for concentration and buffering and add fresh 10% Formalin as needed to bring back into spec. Simple to use, very cost effective and the units are custom made to meet your volume of waste. William DeSalvo, B.S., HTL(ASCP) > Date: Fri, 10 Sep 2010 13:13:13 -0500 > From: tchilton@kumc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] solvent recycling > > I am researching information on solvent recovery systems and could use some help specifically on B/R Instruments. We currently use the CBG Biotech recycler for alcohol use only but have been given funds from our environment of safety department to purchase two solvent recyclers to be used for alcohol, xylene, and formalin. If anyone is using the B/R 9700 ProCycler Advantage to regulate all three waste solvents in one system I would greatly appreciate any feedback. > > Thanks in advance, > > Timothy Chilton, HT (ASCP) > Histology Supervisor > University of Kansas Hospital > 3901 Rainbow Blvd > Kansas City, Kansas 66160 > > phone: (913) 588-1134 > fax: (913) 588-8780 > > tchilton@kumc.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nalini.Makhijani <@t> va.gov Fri Sep 10 15:56:44 2010 From: Nalini.Makhijani <@t> va.gov (Makhijani, Nalini S) Date: Fri Sep 10 16:01:36 2010 Subject: [Histonet] RE: Ventana vs. Leica In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E6D@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E6D@EXCHMBC2.ad.ah.local> Message-ID: <715FA772CEA81A47B1A762AB6E45123A07E5C9C0@VHAV22MSGA3.v22.med.va.gov> Jan, What a wonderful way to put everything in perspective! Definitely one of the most wise quotes by the timeless Beatles! I've been repeating the "Life is very short..." mantra all day. Thank you. On a similar vein is their "Let it be, let it be.." words. Any body else with Beatle words to live (sing) by? Nalini -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, September 08, 2010 8:26 AM To: 'Nails, Felton'; 'Maria Katleba'; histonet Subject: [Histonet] RE: Ventana vs. Leica In My opinion (maybe we all need to start prefacing our comments with this), all of the Ventana reps I have encountered are very professional and would NEVER write something lilke this about a customer. I'm sure if it happened he would be fired on the spot. Perfect time for one of my favorite quotes of all time "Life is very short and there's no time for fussing and fighting my friends." -Lennon&Mccartney Jan Mahoney Omaha From SSCALISE <@t> beaumonthospitals.com Fri Sep 10 16:29:35 2010 From: SSCALISE <@t> beaumonthospitals.com (Sharon Scalise) Date: Fri Sep 10 16:29:50 2010 Subject: [Histonet] LIS, HIS question In-Reply-To: <4C8A0D69.59AC.00AF.0@mercyhealth.com> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E79@EXCHMBC2.ad.ah.local><73A7ED895EE0C24D9267ED814911DF191773F47B@exchange.cmc-nh.org><4C8A0D69.59AC.00AF.0@mercyhealth.com> Message-ID: <4C8A6AFE.1CA9.00C8.1@beaumonthospitals.com> We will be going live with Soft next March and I am looking for anyone currently using SoftPath to discuss some issues with. We are also interested in anyone using SoftPath that is doing immunohistochemistry. If you will be at the NSH meeting in Seattle we would love to meet with you and share information. Sharon E. Scalise, HTL (ASCP) Histology Supervisor William Beaumont Hospital Royal Oak, MI 48073 248 898-5981 sscalise@beaumonthospitals.com From YuJ2 <@t> upmc.edu Fri Sep 10 16:45:58 2010 From: YuJ2 <@t> upmc.edu (Yu, Jian) Date: Fri Sep 10 16:46:07 2010 Subject: [Histonet] Microm HM330 In-Reply-To: References: Message-ID: I have a used HM330 which has been working well for past several years. Recently, the quick release clamp does not lock the knife/holder down well, which is causing problems in knife consumption and quality of sections. I paid ~$3000 about 4 years ago for this one. Does anyone know whether anyone services this and sell replacement parts, or I am better off get another used one. I have a small research lab with 3-4 people using the microtome for cutting mouse tissues, not on a daily basis. I am at University of Pittsburgh. Any info will be greatly appreciated. Thanks and have a great weekend, Jian Yu **************************************************** Jian Yu, Ph.D. Assistant Professor of Pathology University of Pittsburgh Cancer Institute Hillman Cancer Center Research Pavilion Office Suite 2.26h, Laboratory 2.43 5117 Centre Avenue, Pittsburgh, PA 15213 ? Phone: 412-623-7786, (Lab) 412-623-3255 Fax:???412-623-7778 Email: yuj2@upmc.edu **************************************************** From sfeher <@t> CMC-NH.ORG Fri Sep 10 18:24:59 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Sep 10 18:25:03 2010 Subject: [Histonet] LIS, HIS question In-Reply-To: <4C8A6AFE.1CA9.00C8.1@beaumonthospitals.com> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43E79@EXCHMBC2.ad.ah.local><73A7ED895EE0C24D9267ED814911DF191773F47B@exchange.cmc-nh.org><4C8A0D69.59AC.00AF.0@mercyhealth.com> <4C8A6AFE.1CA9.00C8.1@beaumonthospitals.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F490@exchange.cmc-nh.org> Sharon, We are using SoftPath with the Bond Max system. I will be presenting WS#50 Designing a LEAN Pathology Lab from Scratch, on Monday, Sept 27 at 8 am. We can meet after the workshop or perhaps it may come up during the workshop. I have quite a few lessons learned and would have, could have, should have's from my experience in bringing Soft in without having an LIS that it was replacing. I would be happy to meet with you to discuss this. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Friday, September 10, 2010 5:30 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: RE: [Histonet] LIS, HIS question We will be going live with Soft next March and I am looking for anyone currently using SoftPath to discuss some issues with. We are also interested in anyone using SoftPath that is doing immunohistochemistry. If you will be at the NSH meeting in Seattle we would love to meet with you and share information. Sharon E. Scalise, HTL (ASCP) Histology Supervisor William Beaumont Hospital Royal Oak, MI 48073 248 898-5981 sscalise@beaumonthospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Fri Sep 10 18:29:24 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Sep 10 18:29:27 2010 Subject: [Histonet] solvent recycling In-Reply-To: References: <4C8A2EE9.002D.0049.1@kumc.edu> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F491@exchange.cmc-nh.org> I agree with William. We are currently using the Creative Waste Solutions recyclers pretty much the same way. One advantage is that Creative Waste will work with you using some pretty creative parameters to lease the recyclers. I suggest getting in touch with them. You will be surprised at the overall cost. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Friday, September 10, 2010 3:51 PM To: tchilton@kumc.edu; histonet Subject: RE: [Histonet] solvent recycling I suggest you look into Creative Waste Solutions Inc. for recycling (actually filtering through a slurry) your Formalin and Alcohols. I have over 8 years experience with the filtering systems. Dirty solution in, clean solution out and there is no breakdown of the chemical, just a cleaning (crystal clear and pure). For alcohol we combine several grades and then re-use them in all dilutions. Fresh alcohol is used for "100%" steps. For the Formalin, we combine filtered, test for concentration and buffering and add fresh 10% Formalin as needed to bring back into spec. Simple to use, very cost effective and the units are custom made to meet your volume of waste. William DeSalvo, B.S., HTL(ASCP) > Date: Fri, 10 Sep 2010 13:13:13 -0500 > From: tchilton@kumc.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] solvent recycling > > I am researching information on solvent recovery systems and could use some help specifically on B/R Instruments. We currently use the CBG Biotech recycler for alcohol use only but have been given funds from our environment of safety department to purchase two solvent recyclers to be used for alcohol, xylene, and formalin. If anyone is using the B/R 9700 ProCycler Advantage to regulate all three waste solvents in one system I would greatly appreciate any feedback. > > Thanks in advance, > > Timothy Chilton, HT (ASCP) > Histology Supervisor > University of Kansas Hospital > 3901 Rainbow Blvd > Kansas City, Kansas 66160 > > phone: (913) 588-1134 > fax: (913) 588-8780 > > tchilton@kumc.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elciba <@t> hotmail.com Sat Sep 11 12:25:28 2010 From: elciba <@t> hotmail.com (ricky hachy) Date: Sat Sep 11 12:25:34 2010 Subject: [Histonet] Reichert Frigocut 2800 Manuals Message-ID: Hello, Does anybody have the user or service manuals for the RIECHERT-JUNG FRIGOCUT 2800 ? Thanks Ricky From Larry_Farrand <@t> vwr.com Sat Sep 11 13:01:07 2010 From: Larry_Farrand <@t> vwr.com (Larry_Farrand@vwr.com) Date: Sat Sep 11 13:01:20 2010 Subject: [Histonet] Larry Farrand is out of the office Message-ID: I will be out of the office starting 09/10/2010 and will not return until 09/20/2010. I will not have access to e-mail. Please contact customer service with any questions. If you have an issue that needs to be addressed, Karen Longo my regional manager can be contacted to assist you (karen_longo@vwr.com or 1_847-412-8900) From Traczyk7 <@t> aol.com Sat Sep 11 18:21:27 2010 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Sat Sep 11 18:21:45 2010 Subject: [Histonet] Flat Ice??? Message-ID: <46abb.58819722.39bd68f7@aol.com> I had the same problem. Now I make sure the pan is covered when I put it in the freezer. I just use a stray plastic container cover and it works out just fine. Dorothy From histotech <@t> imagesbyhopper.com Sat Sep 11 21:22:50 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Sep 11 21:23:06 2010 Subject: [Histonet] Flat Ice??? In-Reply-To: Message-ID: <742760BA93F24A9B851A6A3376204083@hopperPC> I much prefer the "wet ice" approach to the more dry variety of the cool trays. The "wet" ice gets my blocks colder and I like the fact that it introduces a small amount of moisture into the already faced block. My blocks are not on the ice for long periods of time, so over-saturating them with water is not an issue. To keep my trays from growing mountains, I have found that if I fill them up 3/4 of the way and let them freeze and then follow up with the other 1/4 later in the day, I get nice, flat little "ice rinks" that I can lay my blocks on. It's not time consuming either, just have to remember to go back and pour more water on them! By the way, this works on ice cube trays as well as steel pans we have. I don't really care for the pans, but some of my techs do. They take a little more work to make them ice rinks. ;o) My $0.02. :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Friday, September 10, 2010 12:03 PM To: msherwood@partners.org; sraibley@yahoo.com; histonet Subject: RE: [Histonet] Flat Ice??? For quality assurance and safety concerns, I would suggest moving away from the "wet ice" and go with a sealed freezing tray. We moved to the small Histo-Cool ice trays w/ tray. http://www.labstore.com/catalog/index.cfm?Category_ID=375 William DeSalvo, B.S., HTL(ASCP) > Date: Fri, 10 Sep 2010 10:58:01 -0400 > From: MSHERWOOD@PARTNERS.ORG > To: sraibley@yahoo.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Flat Ice??? > CC: > > We use a styrofoam container and have no problem with it freezing > flat. Are you containers sitting flat in the freezer? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan > Raibley > Sent: Friday, September 10, 2010 10:52 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Flat Ice??? > > Hello! I would greatly appreciate any tips or tricks to getting ice to > freeze flat in the pan! We are tired of opening the freezer to see > mountains that have formed in our pans overnight, making it hard to > try and fit all the blocks we need to microtome on them. We have two > freezers and they both have this problem. Help please! Thanks! > > > Susan Bincsik, HT (ASCP) > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom > it is addressed. If you believe this e-mail was sent to you in error > and the e-mail contains patient information, please contact the > Partners Compliance HelpLine at http://www.partners.org/complianceline > . If the e-mail was sent to you in error but does not contain patient > information, please contact the sender and properly dispose of the > e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3117 - Release Date: 09/08/10 06:07:00 From c_m_hernandezjr <@t> yahoo.com Sat Sep 11 22:57:11 2010 From: c_m_hernandezjr <@t> yahoo.com (Carlos Hernandez) Date: Sat Sep 11 22:57:15 2010 Subject: [Histonet] Sakura X50 Microwave Tissue Processor Message-ID: <399458.78523.qm@web31804.mail.mud.yahoo.com> I'm looking for some feedback (pros & cons) from anyone using the Sakura X50. Especially any derm labs that have experience with it. Thanks, Carlos From ychen9 <@t> kent.edu Sun Sep 12 00:47:11 2010 From: ychen9 <@t> kent.edu (CHEN, YIJING) Date: Sun Sep 12 00:47:19 2010 Subject: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded) Message-ID: Hi, We are having difficulty sectioning paraffin-embedded decalcified adult mouse autopods (paws). The tissues shatter as soon as they hit the blade when sectioned. The autopods were soaked in CalEX for 4 days at room temp and felt extremely soft before embedding, suggesting effective decalcification. We use the Sturkey EXTREMUS low profile disposable knives on our microtome. These knives are said to be coated with the hardest nitride coating available. Any suggestions will be greatly appreciated. Sincerely, Yijing From rjbuesa <@t> yahoo.com Sun Sep 12 09:28:49 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Sep 12 09:28:54 2010 Subject: [Histonet] Sakura X50 Microwave Tissue Processor In-Reply-To: <399458.78523.qm@web31804.mail.mud.yahoo.com> Message-ID: <626067.66375.qm@web65716.mail.ac4.yahoo.com> Just a rhetorical question: do you need such an expensive instrument to process skin Bxs? Ren? J. --- On Sat, 9/11/10, Carlos Hernandez wrote: From: Carlos Hernandez Subject: [Histonet] Sakura X50 Microwave Tissue Processor To: histonet@lists.utsouthwestern.edu Date: Saturday, September 11, 2010, 11:57 PM I'm looking for some feedback (pros & cons) from anyone using the Sakura X50. Especially any derm labs that have experience with it. Thanks, Carlos ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> wfubmc.edu Sun Sep 12 12:11:19 2010 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Sun Sep 12 13:40:04 2010 Subject: [Histonet] Re: Histonet Digest, Vol 82, Issue 14 In-Reply-To: References: Message-ID: Any suggestions for staining nets for floating brain sections? I've looked at netwells, cell strainers and have even considered making my own. Most commercial options are way too expensive. I'd love to hear what everyone uses and if there is an easy way to make them. Thanks! On Sep 12, 2010, at 1:01 PM, "histonet-request@lists.utsouthwestern.edu" wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Reichert Frigocut 2800 Manuals (ricky hachy) > 2. Larry Farrand is out of the office (Larry_Farrand@vwr.com) > 3. Re: Flat Ice??? (Traczyk7@aol.com) > 4. RE: Flat Ice??? (histotech@imagesbyhopper.com) > 5. Sakura X50 Microwave Tissue Processor (Carlos Hernandez) > 6. Trouble shooting decalcified bone sections (paraffin > embedded) (CHEN, YIJING) > 7. Re: Sakura X50 Microwave Tissue Processor (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 11 Sep 2010 17:25:28 +0000 > From: ricky hachy > Subject: [Histonet] Reichert Frigocut 2800 Manuals > To: Histonet > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hello, > > Does anybody have the user or service manuals for the RIECHERT-JUNG FRIGOCUT 2800 ? > > Thanks > Ricky > > ------------------------------ > > Message: 2 > Date: Sat, 11 Sep 2010 14:01:07 -0400 > From: Larry_Farrand@vwr.com > Subject: [Histonet] Larry Farrand is out of the office > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 09/10/2010 and will not return until > 09/20/2010. > > I will not have access to e-mail. Please contact customer service with any > questions. If you have an issue that needs to be addressed, Karen Longo my > regional manager can be contacted to assist you (karen_longo@vwr.com or > 1_847-412-8900) > > > > > ------------------------------ > > Message: 3 > Date: Sat, 11 Sep 2010 19:21:27 EDT > From: Traczyk7@aol.com > Subject: Re: [Histonet] Flat Ice??? > To: sraibley@yahoo.com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46abb.58819722.39bd68f7@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > I had the same problem. Now I make sure the pan is covered when I put it > in the freezer. I just use a stray plastic container cover and it works > out just fine. > Dorothy > > > ------------------------------ > > Message: 4 > Date: Sat, 11 Sep 2010 22:22:50 -0400 > From: > Subject: RE: [Histonet] Flat Ice??? > To: "'WILLIAM DESALVO'" , > , , "'histonet'" > > Message-ID: <742760BA93F24A9B851A6A3376204083@hopperPC> > Content-Type: text/plain; charset=US-ASCII > > I much prefer the "wet ice" approach to the more dry variety of the cool > trays. The "wet" ice gets my blocks colder and I like the fact that it > introduces a small amount of moisture into the already faced block. My > blocks are not on the ice for long periods of time, so over-saturating them > with water is not an issue. > > To keep my trays from growing mountains, I have found that if I fill them up > 3/4 of the way and let them freeze and then follow up with the other 1/4 > later in the day, I get nice, flat little "ice rinks" that I can lay my > blocks on. It's not time consuming either, just have to remember to go back > and pour more water on them! By the way, this works on ice cube trays as > well as steel pans we have. I don't really care for the pans, but some of > my techs do. They take a little more work to make them ice rinks. ;o) > > My $0.02. :o) > > Michelle > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM > DESALVO > Sent: Friday, September 10, 2010 12:03 PM > To: msherwood@partners.org; sraibley@yahoo.com; histonet > Subject: RE: [Histonet] Flat Ice??? > > > > For quality assurance and safety concerns, I would suggest moving away from > the "wet ice" and go with a sealed freezing tray. We moved to the small > Histo-Cool ice trays w/ tray. > > http://www.labstore.com/catalog/index.cfm?Category_ID=375 > > William DeSalvo, B.S., HTL(ASCP) > > > > > >> Date: Fri, 10 Sep 2010 10:58:01 -0400 >> From: MSHERWOOD@PARTNERS.ORG >> To: sraibley@yahoo.com; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Flat Ice??? >> CC: >> >> We use a styrofoam container and have no problem with it freezing >> flat. Are you containers sitting flat in the freezer? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan >> Raibley >> Sent: Friday, September 10, 2010 10:52 AM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Flat Ice??? >> >> Hello! I would greatly appreciate any tips or tricks to getting ice to >> freeze flat in the pan! We are tired of opening the freezer to see >> mountains that have formed in our pans overnight, making it hard to >> try and fit all the blocks we need to microtome on them. We have two >> freezers and they both have this problem. Help please! Thanks! >> >> >> Susan Bincsik, HT (ASCP) >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> The information in this e-mail is intended only for the person to whom >> it is addressed. If you believe this e-mail was sent to you in error >> and the e-mail contains patient information, please contact the >> Partners Compliance HelpLine at http://www.partners.org/complianceline >> . If the e-mail was sent to you in error but does not contain patient >> information, please contact the sender and properly dispose of the >> e-mail. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.441 / Virus Database: 271.1.1/3117 - Release Date: 09/08/10 > 06:07:00 > > > > > ------------------------------ > > Message: 5 > Date: Sat, 11 Sep 2010 20:57:11 -0700 (PDT) > From: Carlos Hernandez > Subject: [Histonet] Sakura X50 Microwave Tissue Processor > To: histonet@lists.utsouthwestern.edu > Message-ID: <399458.78523.qm@web31804.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > I'm looking for some feedback (pros & cons) from anyone using the Sakura X50. > Especially any derm labs that have experience with it. > > Thanks, > > Carlos > > > > > ------------------------------ > > Message: 6 > Date: Sun, 12 Sep 2010 01:47:11 -0400 > From: "CHEN, YIJING" > Subject: [Histonet] Trouble shooting decalcified bone sections > (paraffin embedded) > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hi, > We are having difficulty sectioning paraffin-embedded decalcified adult > mouse autopods (paws). The tissues shatter as soon as they hit the blade > when sectioned. > > The autopods were soaked in CalEX for 4 days at room temp and felt extremely > soft before embedding, suggesting effective decalcification. We use the > Sturkey EXTREMUS low profile disposable knives on our microtome. These > knives are said to be coated with the hardest nitride coating available. > Any suggestions will be greatly appreciated. > Sincerely, > Yijing > > > > > ------------------------------ > > Message: 7 > Date: Sun, 12 Sep 2010 07:28:49 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Sakura X50 Microwave Tissue Processor > To: histonet@lists.utsouthwestern.edu, Carlos Hernandez > > Message-ID: <626067.66375.qm@web65716.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Just a rhetorical question: do you need such an expensive instrument to process skin Bxs? > Ren? J. > > --- On Sat, 9/11/10, Carlos Hernandez wrote: > > > From: Carlos Hernandez > Subject: [Histonet] Sakura X50 Microwave Tissue Processor > To: histonet@lists.utsouthwestern.edu > Date: Saturday, September 11, 2010, 11:57 PM > > > I'm looking for some feedback (pros & cons) from anyone using the Sakura X50. > Especially any derm labs that have experience with it. > > Thanks, > > Carlos > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 82, Issue 14 > **************************************** From gayle.callis <@t> bresnan.net Sun Sep 12 18:23:53 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sun Sep 12 18:24:02 2010 Subject: [Histonet] Tissue shrinkage revisited, Glycol Methacrylate embedding Message-ID: <000001cb52d1$9272a320$b757e960$@callis@bresnan.net> During reorganizing files, I came across this elegant publication addressing shrinkage of liver samples embedded in different mixtures of GMA. It was an extensive, excellent study that discussed shrinkage or swelling during NBF fixation (shrinkage), dehydration (shrinkage), dehydration followed by infiltration (swelling), and the final polymerization (shrinkage). There were also recommendations for using correction factors when doing morphometrical and stereological studies, including a reference on the latter two studies. Hanstede JG, Gerrits PO. The effects of embedding in water soluble plastics on the final dimensions of liver sections. J Microscopy 131(1): 79-86, 1983. If anyone would like to have the pdf, I will be happy to forward it to you. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From wdesalvo.cac <@t> hotmail.com Sun Sep 12 19:13:25 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Sun Sep 12 19:13:30 2010 Subject: [Histonet] Sakura X50 Microwave Tissue Processor In-Reply-To: <626067.66375.qm@web65716.mail.ac4.yahoo.com> References: <399458.78523.qm@web31804.mail.mud.yahoo.com>, <626067.66375.qm@web65716.mail.ac4.yahoo.com> Message-ID: Carlos, I think Rene is missing the point, anyone that has substantial derm work will know that workflow is king when the client demand is 1 day TAT. There are many conventional processing instruments and methods, but I think you are right to consider rapid continuous processing. You need a continuous process flow to be the most cost effective in processing the skin biopsies through the lab. You will be ale to remove large batching, waiting for instruments to clean and xylene from your processing. You will no longer wait until the next day to turn out cases to the pathologists or have to make your staff come in at 3-4 am, when they can work more desirable hours. Move to a continuous process flow and the cost of a LEAN instrument that functions efficiently in the process is worth every penny. I have used the X120 for 5+ yrs and there is great value in rapid, continuous processing. William DeSalvo, B.S., HTL(ASCP) > Date: Sun, 12 Sep 2010 07:28:49 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; c_m_hernandezjr@yahoo.com > Subject: Re: [Histonet] Sakura X50 Microwave Tissue Processor > CC: > > Just a rhetorical question: do you need such an expensive instrument to process skin Bxs? > Ren? J. > > --- On Sat, 9/11/10, Carlos Hernandez wrote: > > > From: Carlos Hernandez > Subject: [Histonet] Sakura X50 Microwave Tissue Processor > To: histonet@lists.utsouthwestern.edu > Date: Saturday, September 11, 2010, 11:57 PM > > > I'm looking for some feedback (pros & cons) from anyone using the Sakura X50. > Especially any derm labs that have experience with it. > > Thanks, > > Carlos > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Sep 13 07:09:48 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Sep 13 07:10:05 2010 Subject: [Histonet] new lab design Message-ID: <5A2BD13465E061429D6455C8D6B40E390EAF72C80B@IBMB7Exchange.digestivespecialists.com> Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From MW <@t> PersonifySearch.com Mon Sep 13 07:46:36 2010 From: MW <@t> PersonifySearch.com (Matthew Ward) Date: Mon Sep 13 07:46:45 2010 Subject: [Histonet] Histotechs Needed!! Message-ID: <004901cb5341$b42f75e0$1c8e61a0$@com> Good Morning, Our team here at Personify has recently been partnered with a World Leader in Cancer Diagnostics who is going through a large expansion due to their recent and continued success in the Immunohistochemistry market. Due to this expansion we are currently seeking Histotechs who have experience working with IHC analyzers that would be interested in Field Support roles. These Positions Offer: - Outstanding Base Salary and Bonus! - Gold Standard Benefits including but not limited to Medical, Cell Phone, Laptop, Car Allowance, Expenses, 401k, Paid Vacation! - Opportunity for Career Advancement! We currently have positions open in the following areas and will have more locations throughout the year! Southeast Florida (Miami/ FT. Lauderdale) Boston/ New England MD/VA/DC NYC/Bronx/NJ TN/MS/AR If you are interested in learning more please contact me directly at 800.875.6188 ext. 103 or mw@personifysearch.com Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From PVerden <@t> UROPARTNERS.COM Mon Sep 13 09:25:47 2010 From: PVerden <@t> UROPARTNERS.COM (Paul Verden) Date: Mon Sep 13 09:25:52 2010 Subject: [Histonet] re: new lab design Message-ID: Congratulations on your move to a new building and the opportunity to have a "fresh" start in a new lab. The first thing I would suggest is to NOT blindly depend on a laboratory design firm to design your lab. Consider the design and operation of your new lab like you would buying a new car. Would you buy a new car from a dealer just because they tell you that they know what they are doing and they know what you need? Or, would you prefer to a car built for you that has all the elements you need to function in the way you want it to function? Second, make sure you are involved in all the design aspects of your laboratory. Don't trust anyone but yourself and your techs to know exactly what you need. Third, and most important, be sure to involve your lab/cyto/histo technologists in the design. No one knows better about how they would like their work area to be designed than the people doing the actual work. You will probably get some of your best ideas from the troops. And, they will greatly appreciate the opportunity to input on the design and have a much greater "buy-in" when all is said and done. Good luck on your endeavor. You will get great advice from your peers on the histonet. Paul Supervisor - Cytology and molecular UroPartners, LLC From Kim.Donadio <@t> bhcpns.org Mon Sep 13 09:52:41 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Mon Sep 13 09:52:53 2010 Subject: [Histonet] Flat Ice??? In-Reply-To: <742760BA93F24A9B851A6A3376204083@hopperPC> Message-ID: Thanks. I am going to try that "ice rink: tip. :-) What I do to keep my blocks from sliding all over the place now is put a kimwipe on my wet ice. They can cool and I also like to hydrate my blocks a little. I feel it gives me a better looking section histologically. my 2 cents as well. ;o) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-bounces@lists.utsouthwestern.edu 09/11/2010 09:22 PM To "'WILLIAM DESALVO'" , , , "'histonet'" cc Subject RE: [Histonet] Flat Ice??? I much prefer the "wet ice" approach to the more dry variety of the cool trays. The "wet" ice gets my blocks colder and I like the fact that it introduces a small amount of moisture into the already faced block. My blocks are not on the ice for long periods of time, so over-saturating them with water is not an issue. To keep my trays from growing mountains, I have found that if I fill them up 3/4 of the way and let them freeze and then follow up with the other 1/4 later in the day, I get nice, flat little "ice rinks" that I can lay my blocks on. It's not time consuming either, just have to remember to go back and pour more water on them! By the way, this works on ice cube trays as well as steel pans we have. I don't really care for the pans, but some of my techs do. They take a little more work to make them ice rinks. ;o) My $0.02. :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Friday, September 10, 2010 12:03 PM To: msherwood@partners.org; sraibley@yahoo.com; histonet Subject: RE: [Histonet] Flat Ice??? For quality assurance and safety concerns, I would suggest moving away from the "wet ice" and go with a sealed freezing tray. We moved to the small Histo-Cool ice trays w/ tray. http://www.labstore.com/catalog/index.cfm?Category_ID=375 William DeSalvo, B.S., HTL(ASCP) > Date: Fri, 10 Sep 2010 10:58:01 -0400 > From: MSHERWOOD@PARTNERS.ORG > To: sraibley@yahoo.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Flat Ice??? > CC: > > We use a styrofoam container and have no problem with it freezing > flat. Are you containers sitting flat in the freezer? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan > Raibley > Sent: Friday, September 10, 2010 10:52 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Flat Ice??? > > Hello! I would greatly appreciate any tips or tricks to getting ice to > freeze flat in the pan! We are tired of opening the freezer to see > mountains that have formed in our pans overnight, making it hard to > try and fit all the blocks we need to microtome on them. We have two > freezers and they both have this problem. Help please! Thanks! > > > Susan Bincsik, HT (ASCP) > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom > it is addressed. If you believe this e-mail was sent to you in error > and the e-mail contains patient information, please contact the > Partners Compliance HelpLine at http://www.partners.org/complianceline > . If the e-mail was sent to you in error but does not contain patient > information, please contact the sender and properly dispose of the > e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3117 - Release Date: 09/08/10 06:07:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From akbitting <@t> geisinger.edu Mon Sep 13 10:11:21 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Sep 13 10:11:30 2010 Subject: [Histonet] SOX-10 Message-ID: <4C8E06D9.2B7F.00C9.1@geisinger.edu> Happy Monday Histonetters, I'm wondering if anyone can share a protocol for running SOX-10 on the Ventana BenchmarkXT? (On FFPE tissue) Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From klauing <@t> lumc.edu Mon Sep 13 10:13:00 2010 From: klauing <@t> lumc.edu (Kristen Lauing) Date: Mon Sep 13 10:13:27 2010 Subject: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded) Message-ID: <4C8DF92C020000FD0001EFDE@gwgwia2.luhs.org> I section EDTA-decalcified mouse tibias frequently, and I find it helps if the paraffin is very very cold during sectioning - if the tissue starts shredding again, I press a large piece of ice to the block for a minute to cool it down without having to remove the block from the microtome. It seems to work well for me, although it may not be the most technically sound way to get the job done. I can usually get a good ribbon of sections this way when I'm having difficulty with a particular sample. I have to use an old microtome and blades because I'm a student at a research institution, without the help of professional histotechs. Our tibias are decalcified in 10% EDTA for 7 days at 4 degrees with agitation with frequent solution changes. Kristen >>> "CHEN, YIJING" 09/12/10 12:50 AM >>> Hi, We are having difficulty sectioning paraffin-embedded decalcified adult mouse autopods (paws). The tissues shatter as soon as they hit the blade when sectioned. The autopods were soaked in CalEX for 4 days at room temp and felt extremely soft before embedding, suggesting effective decalcification. We use the Sturkey EXTREMUS low profile disposable knives on our microtome. These knives are said to be coated with the hardest nitride coating available. Any suggestions will be greatly appreciated. Sincerely, Yijing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jhisto37 <@t> yahoo.com Mon Sep 13 10:57:13 2010 From: jhisto37 <@t> yahoo.com (Jessica Piche) Date: Mon Sep 13 10:57:17 2010 Subject: [Histonet] Napsin A Message-ID: <107374.23832.qm@web120601.mail.ne1.yahoo.com> Hello Everyone, We are in the process of working up Napsin A. I was wondering what?tissue others?are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT From gayle.callis <@t> bresnan.net Mon Sep 13 11:23:32 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Sep 13 11:23:42 2010 Subject: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded) In-Reply-To: <4C8DF92C020000FD0001EFDE@gwgwia2.luhs.org> References: <4C8DF92C020000FD0001EFDE@gwgwia2.luhs.org> Message-ID: <002401cb5360$0407ffb0$0c17ff10$@callis@bresnan.net> YiJing, You could be under- processing the mouse paws. We extend processing time for mouse paws during dehydration, clearing and especially infiltration with paraffin and done with vacuum. Poor paraffin infiltration can really create shredding problems. There is a simple, cheap way to check endpoint of decalcification when decalcifying bones. It is called weight loss/weight gain method and works for both EDTA and any acid decalcification protocols. Endpoint determination is important to know when calcium is totally removed so processing and microtomy problems do not occur. Bending or "feeling soft" generally is a poor way to determine total calcium removal as small calcium deposits are not detectable this way. X-ray is the most sensitive, but not everyone has the technology/equipment for that. Chemical endpoint testing is also simple and easy to do. I doubt the blades are the problem, but more likely decalcification and processing. I would be happy to send the weight loss/weight gain and chemical test methods to you. Using a harder paraffin helps with bone, Tissue Prep 2 (Thermo Scientific under Fisher Scientific label) is one of several available although other paraffins should work if infiltration is done properly. You did not provide a processing schedule for these decalcified mouse paws. Is this done manually or on an automate processor? Times in each solution? Time of paraffin infiltration? Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen Lauing Sent: Monday, September 13, 2010 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded) I section EDTA-decalcified mouse tibias frequently, and I find it helps if the paraffin is very very cold during sectioning - if the tissue starts shredding again, I press a large piece of ice to the block for a minute to cool it down without having to remove the block from the microtome. It seems to work well for me, although it may not be the most technically sound way to get the job done. I can usually get a good ribbon of sections this way when I'm having difficulty with a particular sample. I have to use an old microtome and blades because I'm a student at a research institution, without the help of professional histotechs. Our tibias are decalcified in 10% EDTA for 7 days at 4 degrees with agitation with frequent solution changes. Kristen >>> "CHEN, YIJING" 09/12/10 12:50 AM >>> Hi, We are having difficulty sectioning paraffin-embedded decalcified adult mouse autopods (paws). The tissues shatter as soon as they hit the blade when sectioned. The autopods were soaked in CalEX for 4 days at room temp and felt extremely soft before embedding, suggesting effective decalcification. We use the Sturkey EXTREMUS low profile disposable knives on our microtome. These knives are said to be coated with the hardest nitride coating available. Any suggestions will be greatly appreciated. Sincerely, Yijing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5447 (20100913) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5447 (20100913) __________ The message was checked by ESET Smart Security. http://www.eset.com From sfeher <@t> CMC-NH.ORG Mon Sep 13 11:28:52 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Sep 13 11:28:58 2010 Subject: [Histonet] new lab design In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EAF72C80B@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E390EAF72C80B@IBMB7Exchange.digestivespecialists.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F4A4@exchange.cmc-nh.org> Hi Linda, We designed one from scratch without having a previous Path Lab in the hospital before. We are doing a workshop to that end at NSH in Seattle (WS 50). If you cannot attend the workshop, I will be happy to help in any that I can. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, September 13, 2010 8:10 AM To: 'Histonet' Subject: [Histonet] new lab design Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Sep 13 11:38:06 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Sep 13 11:38:11 2010 Subject: [Histonet] new lab design In-Reply-To: <73A7ED895EE0C24D9267ED814911DF191773F4A4@exchange.cmc-nh.org> References: <5A2BD13465E061429D6455C8D6B40E390EAF72C80B@IBMB7Exchange.digestivespecialists.com> <73A7ED895EE0C24D9267ED814911DF191773F4A4@exchange.cmc-nh.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5DF8373@LRGHEXVS1.practice.lrgh.org> FYI, I have been to Steve's lab. They have a great layout. A lot of time and effort was spent in the design of it and it shows. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, September 13, 2010 12:29 PM To: Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design Hi Linda, We designed one from scratch without having a previous Path Lab in the hospital before. We are doing a workshop to that end at NSH in Seattle (WS 50). If you cannot attend the workshop, I will be happy to help in any that I can. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, September 13, 2010 8:10 AM To: 'Histonet' Subject: [Histonet] new lab design Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From sfeher <@t> CMC-NH.ORG Mon Sep 13 11:38:46 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Sep 13 11:38:50 2010 Subject: [Histonet] new lab design In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323D5DF8373@LRGHEXVS1.practice.lrgh.org> References: <5A2BD13465E061429D6455C8D6B40E390EAF72C80B@IBMB7Exchange.digestivespecialists.com> <73A7ED895EE0C24D9267ED814911DF191773F4A4@exchange.cmc-nh.org> <38667E7FB77ECD4E91BFAEB8D986386323D5DF8373@LRGHEXVS1.practice.lrgh.org> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F4A6@exchange.cmc-nh.org> Thanks Tim! Steve -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Monday, September 13, 2010 12:38 PM To: Feher, Stephen; Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design FYI, I have been to Steve's lab. They have a great layout. A lot of time and effort was spent in the design of it and it shows. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, September 13, 2010 12:29 PM To: Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design Hi Linda, We designed one from scratch without having a previous Path Lab in the hospital before. We are doing a workshop to that end at NSH in Seattle (WS 50). If you cannot attend the workshop, I will be happy to help in any that I can. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, September 13, 2010 8:10 AM To: 'Histonet' Subject: [Histonet] new lab design Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Timothy.Morken <@t> ucsfmedctr.org Mon Sep 13 11:42:21 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Sep 13 11:42:36 2010 Subject: [Histonet] new lab design In-Reply-To: <73A7ED895EE0C24D9267ED814911DF191773F4A4@exchange.cmc-nh.org> References: <5A2BD13465E061429D6455C8D6B40E390EAF72C80B@IBMB7Exchange.digestivespecialists.com> <73A7ED895EE0C24D9267ED814911DF191773F4A4@exchange.cmc-nh.org> Message-ID: <1AAF670737F193429070841C6B2ADD4C025501012F@EXMBMCB15.ucsfmedicalcenter.org> Steve's workshop at NSH last year was a good intro to lab design. The most significant take-away was that they did many, many modifications on paper before they ever did anything for real...and all with LEAN principle driving the design. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, September 13, 2010 9:29 AM To: Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design Hi Linda, We designed one from scratch without having a previous Path Lab in the hospital before. We are doing a workshop to that end at NSH in Seattle (WS 50). If you cannot attend the workshop, I will be happy to help in any that I can. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, September 13, 2010 8:10 AM To: 'Histonet' Subject: [Histonet] new lab design Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Sep 13 12:29:58 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Sep 13 12:32:26 2010 Subject: [Histonet] Napsin A In-Reply-To: <107374.23832.qm@web120601.mail.ne1.yahoo.com> References: <107374.23832.qm@web120601.mail.ne1.yahoo.com> Message-ID: We are using a Lung Adenocarcinoma as a daily control. Although I have noticed that normal lung macrophages will pick it up. We ran our validation of the antibody using a multi tumor block (contains various tumors from various organs) and a multi normal tissue block to ensure that it stained only the expected things and not anything else. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche [jhisto37@yahoo.com] Sent: Monday, September 13, 2010 11:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Napsin A Hello Everyone, We are in the process of working up Napsin A. I was wondering what tissue others are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Herrick.James <@t> mayo.edu Mon Sep 13 12:47:36 2010 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Mon Sep 13 12:47:47 2010 Subject: FW: [Histonet] Liver Processing Question Message-ID: <7267A64D75F58241B577876D8A885631038FE2AD@msgebe41> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. (Jim) Sent: Friday, September 10, 2010 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Liver Processing Question Hello everyone, I am trying to process and embed liver tissue in MMA. I used the same protocol that has worked very well a hundred times over, for harder tissues, but when used on the liver tissue, polymerized into a relatively soft and rubbery textured plastic for one of the two specimens (the second of the two also being too soft to properly section). If anyone would have any ideas/suggestions or protocols for me to follow, it would be very much appreciated. Thanks and have a great day!! Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Mon Sep 13 13:12:59 2010 From: jstaruk <@t> masshistology.com (jstaruk) Date: Mon Sep 13 13:12:44 2010 Subject: [Histonet] new lab design In-Reply-To: <73A7ED895EE0C24D9267ED814911DF191773F4A6@exchange.cmc-nh.org> Message-ID: <97E76349977349218D89C43E81C21E82@JimPC> We recently built our new facility from the ground up. Having worked in various histology labs, I knew what works and what doesn't work. Planning the lab around work-flow is crucial. I started the design by simply writing the name of each room I wanted constructed on a sticky-note. These were initially all the same size. Then I placed each room where I wanted it so work would flow from room to room. Later, I adjusted the sizes to fit the square footage of the outside walls. I even planned on a few through the wall pass-throughs that I knew would help in work-flow. As the plans began to develop, I added where I wanted electrical plugs, lights, sinks, etc. When the architect drew up the official plans, I was still able to adjust some walls until everything was perfect. It took about 3 months to plan the lab and it's perfect for our work load. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, September 13, 2010 8:10 AM To: 'Histonet' Subject: [Histonet] new lab design Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com yahoo.com Mon Sep 13 13:26:55 2010 From: jhisto37 <@t> yahoo.com (Jessica Piche) Date: Mon Sep 13 13:27:02 2010 Subject: [Histonet] Napsin A In-Reply-To: References: <107374.23832.qm@web120601.mail.ne1.yahoo.com> Message-ID: <500742.57158.qm@web120618.mail.ne1.yahoo.com> Hi Loralee, Thanks for your response. What did you use for the multi normal tissue block? Kidney? Also for the multi-tumor block did you just use tumors from organs that? you knew were negative for Napsin or did you use both positive and negative tumors? Thanks, Jessica ________________________________ From: "McMahon, Loralee A" To: Jessica Piche ; "histonet@lists.utsouthwestern.edu" Sent: Mon, September 13, 2010 1:29:58 PM Subject: RE: [Histonet] Napsin A We are using a Lung Adenocarcinoma as a daily control.? Although I have noticed that normal lung macrophages will pick it up. We ran our validation of the antibody using a multi tumor block (contains various tumors from various organs) and a multi normal tissue block to ensure that it stained only the expected things and not anything else.? Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche [jhisto37@yahoo.com] Sent: Monday, September 13, 2010 11:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Napsin A Hello Everyone, We are in the process of working up Napsin A. I was wondering what tissue others are using for multi tissue blocks to validate the anitbody? Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital, CT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Mon Sep 13 14:47:56 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Sep 13 14:48:01 2010 Subject: [Histonet] coverslip removal Message-ID: I am using aqueous mounting media, faramount, for the first time. The slides I coverslipped several days ago look dry. Will soaking in water remove the coverslip? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From marktarango <@t> gmail.com Mon Sep 13 15:52:22 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Sep 13 15:52:26 2010 Subject: [Histonet] Napsin A In-Reply-To: <107374.23832.qm@web120601.mail.ne1.yahoo.com> References: <107374.23832.qm@web120601.mail.ne1.yahoo.com> Message-ID: Hi Jessica, Our control for this antibody is normal lung, adenocarcinoma of the lung, and thyroid. We use this same block for other stains too (TTF-1, sufactant-A, EMA, etc.) but if I were going to make a control block specific for this antibody, I would want to include a squamous cell carcinoma of the lung to show that it's negative for napsin-A. You may see a very tiny amount of staining on the squamous cell carcinomas where the pulmonary-aveolar macrophages are eating up the keratin, but the tumor cells will be negative. Ovarian carcinomas can also stain. I would include a few of those too so the docs can see what that looks like. I would also make sure to include thyroid and thyroid carcinomas. With a particular vendor's antibody, napsin-a seems to stain thyroid (I don't want to name names), but with Novocastra's antibody it doesn't. It was helpful to our pathologists to have an antibody that doesn't stain thyroid. Thanks Mark On Mon, Sep 13, 2010 at 8:57 AM, Jessica Piche wrote: > Hello Everyone, > > We are in the process of working up Napsin A. I was wondering what tissue > others are using for multi tissue blocks to validate the anitbody? Thanks, > > > Jessica Piche-Grocki, HT(ASCP) > Waterbury Hospital, CT > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From garret.t.miyamoto <@t> us.army.mil Mon Sep 13 15:58:43 2010 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Mon Sep 13 15:58:54 2010 Subject: [Histonet] Re: Solvent Recycler In-Reply-To: <0L8L000LREB1ZHA0@mail23.us.army.mil> References: <0L8L000LREB1ZHA0@mail23.us.army.mil> Message-ID: Timothy, We have the B/R 9700 ProCycler to recycle alcohol, xylene, and formalin. The Advantage must be a newer model. About 70 gal of alcohol, 40 gal of xylene, and 20 gal of formalin is recycled every 3 months. This recycler is easy to use and works well for us. As with any equipment, you need to maintain it by doing pm checks (replacing parts) as needed. Garret ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Saturday, September 11, 2010 7:09 am Subject: Histonet Digest, Vol 82, Issue 13 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: H&E+ Alcian Blue (Kim.Donadio@bhcpns.org) > 2. solvent recycling (Timothy Chilton) > 3. Liver Processing Question (Herrick, James L. (Jim)) > 4. RE: solvent recycling (WILLIAM DESALVO) > 5. RE: RE: Ventana vs. Leica (Makhijani, Nalini S) > 6. RE: LIS, HIS question (Sharon Scalise) > 7. Microm HM330 (Yu, Jian) > 8. RE: LIS, HIS question (Feher, Stephen) > 9. RE: solvent recycling (Feher, Stephen) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Fri, 10 Sep 2010 12:46:11 -0500 > From: Kim.Donadio@bhcpns.org > Subject: Re: [Histonet] H&E+ Alcian Blue > To: Deborah Faichney < > Cc: "histonet@lists.utsouthwestern.edu" > <, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > < > Content-Type: text/plain; charset="US-ASCII" > > It wont remain turquoise as long as you use the eosin. At least this is my > experience. I have known some Pathologist to read the mucin that way > (purple). > > > > > Kim Donadio > Pathology Supervisor > Baptist Hospital > 1000 W Moreno St. > Pensacola FL 32501 > Phone (850) 469-7718 > Fax (850) 434-4996 > > > > Deborah Faichney < > Sent by: histonet-bounces@lists.utsouthwestern.edu > 09/09/2010 03:44 AM > > To > "histonet@lists.utsouthwestern.edu" < > cc > > Subject > [Histonet] H&E+ Alcian Blue > > > > > > > Hello all, > > I have a request to carry out a combined staining with H&E + Alcian Blue > pH2.5. I have tried in vain to get this to work but regardless of the > order of staining the end result is dark blue/purple mucin. I have > carried out a parallel experiment whereby the staining has been checked > microscopically then stopped after each of the dyes. (Thus giving 3 > slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really > nicely stained but as soon as the eosin is added (using 2 different stains > and a variety of times) the mucin staining looks similar to the nuclear > stain. I am expecting the alcian blue to remain turquoise: should it? > > For information, I am trying to stain salmon intestine at 5 microns for > using the following: > > Alcian Blue 8GX (certified), pH has been checked > Haematoxylin Z (Cellpath Uk) > 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. > > Thanks from a frustrated technician! > > Debbie Faichney > Histopathology > Institute of Aquaculture > University of Stirling > Scotland > UK > > > > > > > > > -- > The Sunday Times Scottish University of the Year 2009/2010 > The University of Stirling is a charity registered in Scotland, > number SC 011159. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----------------------------------------- > All electronic data transmissions originating from or sent to > Baptist Health Care Corporation (BHC) are subject to monitoring. > This message along with any attached data, are the confidential and > proprietary communications of BHC and are intended to be received > only by the individual or individuals to whom the message has been > addressed. If the reader of this message is not the intended > recipient, please take notice that any use, copying, printing, > forwarding or distribution of this message, in any form, is > strictly prohibited and may violate State or Federal Law. If you > have received this transmission in error, please delete or destroy > all copies of this message. For questions, contact the BHC Privacy > Officer at (850) 434-4472. Rev.10/07. > > ------------------------------ > > Message: 2 > Date: Fri, 10 Sep 2010 13:13:13 -0500 > From: "Timothy Chilton" < > Subject: [Histonet] solvent recycling > To: < > Message-ID: < > Content-Type: text/plain; charset=US-ASCII > > I am researching information on solvent recovery systems and could use some help specifically on B/R Instruments. We currently use the CBG Biotech recycler for alcohol use only but have been given funds from our environment of safety department to purchase two solvent recyclers to be used for alcohol, xylene, and formalin. If anyone is using the B/R 9700 ProCycler Advantage to regulate all three waste solvents in one system I would greatly appreciate any feedback. > > Thanks in advance, > > Timothy Chilton, HT (ASCP) > Histology Supervisor > University of Kansas Hospital > 3901 Rainbow Blvd > Kansas City, Kansas 66160 > > phone: (913) 588-1134 > fax: (913) 588-8780 > > tchilton@kumc.edu > > > > > ------------------------------ > > Message: 3 > Date: Fri, 10 Sep 2010 14:33:58 -0500 > From: "Herrick, James L. (Jim)" < > Subject: [Histonet] Liver Processing Question > To: < > Message-ID: < > Content-Type: text/plain; charset="US-ASCII" > > Hello everyone, > > I am trying to process and embed liver tissue in MMA. I used the same > protocol that has worked very well a hundred times over, for harder > tissues, but when used on the liver tissue, polymerized into a > relatively soft and rubbery textured plastic for one of the two > specimens (the second of the two also being too soft to properly > section). If anyone would have any ideas/suggestions or protocols for me > to follow, it would be very much appreciated. Thanks and have a great > weekend!! > > Jim > > > > > ------------------------------ > > Message: 4 > Date: Fri, 10 Sep 2010 13:51:21 -0600 > From: WILLIAM DESALVO < > Subject: RE: [Histonet] solvent recycling > To: <, histonet < > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > > I suggest you look into Creative Waste Solutions Inc. for recycling (actually filtering through a slurry) your Formalin and Alcohols. I have over 8 years experience with the filtering systems. Dirty solution in, clean solution out and there is no breakdown of the chemical, just a cleaning (crystal clear and pure). For alcohol we combine several grades and then re-use them in all dilutions. Fresh alcohol is used for "100%" steps. For the Formalin, we combine filtered, test for concentration and buffering and add fresh 10% Formalin as needed to bring back into spec. Simple to use, very cost effective and the units are custom made to meet your volume of waste. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > Date: Fri, 10 Sep 2010 13:13:13 -0500 > > From: tchilton@kumc.edu > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] solvent recycling > > > > I am researching information on solvent recovery systems and could use some help specifically on B/R Instruments. We currently use the CBG Biotech recycler for alcohol use only but have been given funds from our environment of safety department to purchase two solvent recyclers to be used for alcohol, xylene, and formalin. If anyone is using the B/R 9700 ProCycler Advantage to regulate all three waste solvents in one system I would greatly appreciate any feedback. > > > > Thanks in advance, > > > > Timothy Chilton, HT (ASCP) > > Histology Supervisor > > University of Kansas Hospital > > 3901 Rainbow Blvd > > Kansas City, Kansas 66160 > > > > phone: (913) 588-1134 > > fax: (913) 588-8780 > > > > tchilton@kumc.edu > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Fri, 10 Sep 2010 13:56:44 -0700 > From: "Makhijani, Nalini S" < > Subject: RE: [Histonet] RE: Ventana vs. Leica > To: "Mahoney,Janice A" <, "Nails, Felton" > <, "Maria Katleba" > <, "histonet" > < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > Jan, > What a wonderful way to put everything in perspective! Definitely one > of the most wise quotes by the timeless Beatles! I've been repeating the > "Life is very short..." mantra all day. Thank you. > On a similar vein is their "Let it be, let it be.." words. > Any body else with Beatle words to live (sing) by? > Nalini > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Wednesday, September 08, 2010 8:26 AM > To: 'Nails, Felton'; 'Maria Katleba'; histonet > Subject: [Histonet] RE: Ventana vs. Leica > > In My opinion (maybe we all need to start prefacing our comments with > this), all of the Ventana reps I have encountered are very professional > and would NEVER write something lilke this about a customer. I'm sure > if it happened he would be fired on the spot. > Perfect time for one of my favorite quotes of all time > "Life is very short and there's no time for fussing and fighting my > friends." > -Lennon&Mccartney > Jan Mahoney > Omaha > > > > > ------------------------------ > > Message: 6 > Date: Fri, 10 Sep 2010 17:29:35 -0400 > From: "Sharon Scalise" < > Subject: RE: [Histonet] LIS, HIS question > To: < > Message-ID: < > Content-Type: text/plain; charset=US-ASCII > > We will be going live with Soft next March and I am looking for anyone currently using SoftPath to discuss some issues with. We are also interested in anyone using SoftPath that is doing immunohistochemistry. If you will be at the NSH meeting in Seattle we would love to meet with you and share information. > > > Sharon E. Scalise, HTL (ASCP) > Histology Supervisor > William Beaumont Hospital > Royal Oak, MI 48073 > 248 898-5981 > sscalise@beaumonthospitals.com > > > ------------------------------ > > Message: 7 > Date: Fri, 10 Sep 2010 17:45:58 -0400 > From: "Yu, Jian" < > Subject: [Histonet] Microm HM330 > To: "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > > Content-Type: text/plain; charset="iso-8859-1" > > I have a used HM330 which has been working well for past several years. Recently, the quick release clamp does not lock the knife/holder down well, which is causing problems in knife consumption and quality of sections. I paid ~$3000 about 4 years ago for this one. Does anyone know whether anyone services this and sell replacement parts, or I am better off get another used one. I have a small research lab with 3-4 people using the microtome for cutting mouse tissues, not on a daily basis. > > I am at University of Pittsburgh. Any info will be greatly appreciated. > > Thanks and have a great weekend, > > Jian Yu > **************************************************** > Jian Yu, Ph.D. > Assistant Professor of Pathology > University of Pittsburgh Cancer Institute > Hillman Cancer Center Research Pavilion > Office Suite 2.26h, Laboratory 2.43 > 5117 Centre Avenue, Pittsburgh, PA 15213 > ? > Phone: 412-623-7786, (Lab) 412-623-3255 > Fax:???412-623-7778 > Email: yuj2@upmc.edu > **************************************************** > > > > > > > ------------------------------ > > Message: 8 > Date: Fri, 10 Sep 2010 19:24:59 -0400 > From: "Feher, Stephen" < > Subject: RE: [Histonet] LIS, HIS question > To: "Sharon Scalise" <, > < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > Sharon, > > We are using SoftPath with the Bond Max system. I will be presenting > WS#50 Designing a LEAN Pathology Lab from Scratch, on Monday, Sept 27 at > 8 am. We can meet after the workshop or perhaps it may come up during > the workshop. I have quite a few lessons learned and would have, could > have, should have's from my experience in bringing Soft in without > having an LIS that it was replacing. > > I would be happy to meet with you to discuss this. > > > Steve > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon > Scalise > Sent: Friday, September 10, 2010 5:30 PM > To: HISTONET@PATHOLOGY.SWMED.EDU > Subject: RE: [Histonet] LIS, HIS question > > We will be going live with Soft next March and I am looking for anyone > currently using SoftPath to discuss some issues with. We are also > interested in anyone using SoftPath that is doing immunohistochemistry. > If you will be at the NSH meeting in Seattle we would love to meet with > you and share information. > > > Sharon E. Scalise, HTL (ASCP) > Histology Supervisor > William Beaumont Hospital > Royal Oak, MI 48073 > 248 898-5981 > sscalise@beaumonthospitals.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Fri, 10 Sep 2010 19:29:24 -0400 > From: "Feher, Stephen" < > Subject: RE: [Histonet] solvent recycling > To: "WILLIAM DESALVO" <, <, > "histonet" < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > I agree with William. We are currently using the Creative Waste > Solutions recyclers pretty much the same way. One advantage is that > Creative Waste will work with you using some pretty creative parameters > to lease the recyclers. I suggest getting in touch with them. You will > be surprised at the overall cost. > > > Steve > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM > DESALVO > Sent: Friday, September 10, 2010 3:51 PM > To: tchilton@kumc.edu; histonet > Subject: RE: [Histonet] solvent recycling > > > I suggest you look into Creative Waste Solutions Inc. for recycling > (actually filtering through a slurry) your Formalin and Alcohols. I have > over 8 years experience with the filtering systems. Dirty solution in, > clean solution out and there is no breakdown of the chemical, just a > cleaning (crystal clear and pure). For alcohol we combine several grades > and then re-use them in all dilutions. Fresh alcohol is used for "100%" > steps. For the Formalin, we combine filtered, test for concentration and > buffering and add fresh 10% Formalin as needed to bring back into spec. > Simple to use, very cost effective and the units are custom made to meet > your volume of waste. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > > Date: Fri, 10 Sep 2010 13:13:13 -0500 > > From: tchilton@kumc.edu > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] solvent recycling > > > > I am researching information on solvent recovery systems and could use > some help specifically on B/R Instruments. We currently use the CBG > Biotech recycler for alcohol use only but have been given funds from our > environment of safety department to purchase two solvent recyclers to be > used for alcohol, xylene, and formalin. If anyone is using the B/R 9700 > ProCycler Advantage to regulate all three waste solvents in one system I > would greatly appreciate any feedback. > > > > Thanks in advance, > > > > Timothy Chilton, HT (ASCP) > > Histology Supervisor > > University of Kansas Hospital > > 3901 Rainbow Blvd > > Kansas City, Kansas 66160 > > > > phone: (913) 588-1134 > > fax: (913) 588-8780 > > > > tchilton@kumc.edu > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 82, Issue 13 > **************************************** From MLashus <@t> pathgroup.com Mon Sep 13 18:24:48 2010 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Mon Sep 13 18:24:52 2010 Subject: [Histonet] Form for validating slides on digital slide scanner Message-ID: <197CD0B02A81F94994A285C59C8AE05C05E4B5F185@pgnexchange.pathgroup.com> We are just getting started with our Digital slide scanner and I am looking for help. Anything you would be willing to share in terms of SOPs, Policies in regards to CAP and validation forms used when you are initially validation the machine would be helpful. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From hlukey <@t> msn.com Mon Sep 13 22:02:47 2010 From: hlukey <@t> msn.com (Hugh Luk) Date: Mon Sep 13 22:02:53 2010 Subject: [Histonet] Snap freezing using a "Dry Shipper?" Message-ID: Hi Histonetters, I need some advice as my group is tasked to procure tissue from surgery sites off-campus. Logistics within those other hospitals do not allow a liquid nitrogen source, and my health and safety folks have asked us to consider using a "Dry Shippers" or cryoport for snap freezing. Does anyone do this? We are concerned that the vapor alone would be insufficient to preserve RNA, DNA, and morphology. We currently use a dewar for liquid nitrogen preservation, but it does not involve the whole 'transport issue'. Research or hospital perspectives are welcome. Thanks in advance and have a good week. Hugh Cancer research center of Hawaii From c_m_hernandezjr <@t> yahoo.com Mon Sep 13 22:38:38 2010 From: c_m_hernandezjr <@t> yahoo.com (Carlos Hernandez) Date: Mon Sep 13 22:38:43 2010 Subject: [Histonet] Sakura X50 Microwave Tissue Processor Message-ID: <753854.2311.qm@web31807.mail.mud.yahoo.com> Thanks to everyone who gave some input on the X50, it helped a lot and has me thinking twice about that particular piece of equipment. Carlos From ree3 <@t> leicester.ac.uk Tue Sep 14 03:23:34 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Tue Sep 14 03:24:01 2010 Subject: [Histonet] new lab design In-Reply-To: <73A7ED895EE0C24D9267ED814911DF191773F4A4@exchange.cmc-nh.org> References: <5A2BD13465E061429D6455C8D6B40E390EAF72C80B@IBMB7Exchange.digestivespecialists.com> <73A7ED895EE0C24D9267ED814911DF191773F4A4@exchange.cmc-nh.org> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8D9568307@EXC-MBX3.cfs.le.ac.uk> Just make sure you have many many many power sockets................. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: 13 September 2010 17:29 To: Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design Hi Linda, We designed one from scratch without having a previous Path Lab in the hospital before. We are doing a workshop to that end at NSH in Seattle (WS 50). If you cannot attend the workshop, I will be happy to help in any that I can. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, September 13, 2010 8:10 AM To: 'Histonet' Subject: [Histonet] new lab design Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Sep 14 03:25:34 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Tue Sep 14 03:25:49 2010 Subject: [Histonet] Flat Ice??? In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24609@PHSXMB30.partners.org> References: <106514.19159.qm@web56003.mail.re3.yahoo.com> <073AE2BEA1C2BA4A8837AB6C4B943D9703E24609@PHSXMB30.partners.org> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8D9568308@EXC-MBX3.cfs.le.ac.uk> If the top is lumpy bumpy, take it out of the "mold" and simply turn it over!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: 10 September 2010 15:58 To: Susan Raibley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flat Ice??? We use a styrofoam container and have no problem with it freezing flat. Are you containers sitting flat in the freezer? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Raibley Sent: Friday, September 10, 2010 10:52 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Flat Ice??? Hello! I would greatly appreciate any tips or tricks to getting ice to freeze flat in the pan! We are tired of opening the freezer to see mountains that have formed in our pans overnight, making it hard to try and fit all the blocks we need to microtome on them. We have two freezers and they both have this problem. Help please! Thanks! ? ? Susan Bincsik, HT (ASCP) ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From je2 <@t> sanger.ac.uk Tue Sep 14 03:45:33 2010 From: je2 <@t> sanger.ac.uk (Jeanne Estabel) Date: Tue Sep 14 03:45:45 2010 Subject: [Histonet] Trouble shooting decalcified bone sections (paraffinembedded) References: <4C8DF92C020000FD0001EFDE@gwgwia2.luhs.org> <002401cb5360$0407ffb0$0c17ff10$@callis@bresnan.net> Message-ID: <34627BB49F43DC43A4A7D90B1B6F739D677BFE@exchsrv4.internal.sanger.ac.uk> Hi YiJing, We are collecting knee joint from mouse and we decalcify in 10% EDTA (pH7.4). Place the pots on a rocking platform under gentle agitation for 72 hours at room temperature. Then change the EDTA solution and place the pots on the rocking platform under gentle agitation for a further 72 hours. The tissues can stay in EDTA for 6 to 7 days. I agree with Gayle that the processing could be also the step to look at. Regards Jeanne Jeanne Estabel, PhD Scientific Manager MGP Histology Operations Manager Wellcome Trust Sanger Institute Cambridge UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: 13 September 2010 17:24 To: 'Kristen Lauing'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Trouble shooting decalcified bone sections (paraffinembedded) YiJing, You could be under- processing the mouse paws. We extend processing time for mouse paws during dehydration, clearing and especially infiltration with paraffin and done with vacuum. Poor paraffin infiltration can really create shredding problems. There is a simple, cheap way to check endpoint of decalcification when decalcifying bones. It is called weight loss/weight gain method and works for both EDTA and any acid decalcification protocols. Endpoint determination is important to know when calcium is totally removed so processing and microtomy problems do not occur. Bending or "feeling soft" generally is a poor way to determine total calcium removal as small calcium deposits are not detectable this way. X-ray is the most sensitive, but not everyone has the technology/equipment for that. Chemical endpoint testing is also simple and easy to do. I doubt the blades are the problem, but more likely decalcification and processing. I would be happy to send the weight loss/weight gain and chemical test methods to you. Using a harder paraffin helps with bone, Tissue Prep 2 (Thermo Scientific under Fisher Scientific label) is one of several available although other paraffins should work if infiltration is done properly. You did not provide a processing schedule for these decalcified mouse paws. Is this done manually or on an automate processor? Times in each solution? Time of paraffin infiltration? Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen Lauing Sent: Monday, September 13, 2010 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded) I section EDTA-decalcified mouse tibias frequently, and I find it helps if the paraffin is very very cold during sectioning - if the tissue starts shredding again, I press a large piece of ice to the block for a minute to cool it down without having to remove the block from the microtome. It seems to work well for me, although it may not be the most technically sound way to get the job done. I can usually get a good ribbon of sections this way when I'm having difficulty with a particular sample. I have to use an old microtome and blades because I'm a student at a research institution, without the help of professional histotechs. Our tibias are decalcified in 10% EDTA for 7 days at 4 degrees with agitation with frequent solution changes. Kristen >>> "CHEN, YIJING" 09/12/10 12:50 AM >>> Hi, We are having difficulty sectioning paraffin-embedded decalcified adult mouse autopods (paws). The tissues shatter as soon as they hit the blade when sectioned. The autopods were soaked in CalEX for 4 days at room temp and felt extremely soft before embedding, suggesting effective decalcification. We use the Sturkey EXTREMUS low profile disposable knives on our microtome. These knives are said to be coated with the hardest nitride coating available. Any suggestions will be greatly appreciated. Sincerely, Yijing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5447 (20100913) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5447 (20100913) __________ The message was checked by ESET Smart Security. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE. From jstaruk <@t> masshistology.com Tue Sep 14 07:23:58 2010 From: jstaruk <@t> masshistology.com (jstaruk) Date: Tue Sep 14 07:23:42 2010 Subject: [Histonet] new lab design In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1E8D9568307@EXC-MBX3.cfs.le.ac.uk> Message-ID: <72A45E102F6C43B3A3B3DF382A7D74F7@JimPC> Yes! We ran power strips along every bench-top _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Tuesday, September 14, 2010 4:24 AM To: 'Feher, Stephen'; Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design Just make sure you have many many many power sockets................. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: 13 September 2010 17:29 To: Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design Hi Linda, We designed one from scratch without having a previous Path Lab in the hospital before. We are doing a workshop to that end at NSH in Seattle (WS 50). If you cannot attend the workshop, I will be happy to help in any that I can. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, September 13, 2010 8:10 AM To: 'Histonet' Subject: [Histonet] new lab design Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Checked by AVG - www.avg.com Version: 9.0.851 / Virus Database: 271.1.1/3119 - Release Date: 09/13/10 14:35:00 From mhale <@t> carisls.com Tue Sep 14 08:04:53 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Sep 14 08:05:01 2010 Subject: [Histonet] Great HT Position in Colorado Message-ID: <6F33D8418806044682A391273399860F052C48E4@s-irv-ex301.PathologyPartners.intranet> Colorado GI Pathology (CGIP) is seeking an experienced histotech to complement its existing staff. CGIP is a specialized lab located in Centennial, CO that provides services to the three largest gastroenterology practices in Denver. Dr. Russell Nash is the chief pathologist and medical director. Tracy Wrinkle is the Operations Manager. The position can be filled by an experienced person who can work from 32 to 40 hours per week. The schedule is basically days, although coverage for other times during vacations will be needed. The pay range starts at $26.00 per hour with benefits. This is a small, congenial work place with an emphasis on team work and cooperation. If you are interested in learning more about the opportunity, please contact Tracy Wrinkle at twrinkle@cogipath.com or call her at (303) 770-4848 for a confidential interview and tour of the facility. Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From jstaruk <@t> masshistology.com Tue Sep 14 08:20:26 2010 From: jstaruk <@t> masshistology.com (jstaruk) Date: Tue Sep 14 08:20:08 2010 Subject: [Histonet] Massachusetts opening In-Reply-To: <6F33D8418806044682A391273399860F052C48E4@s-irv-ex301.PathologyPartners.intranet> Message-ID: <8681A05321884B83BEEF964A40B413FB@JimPC> HISTOLOGIST - Mass Histology Service, Inc. is a busy, well established, rapidly growing, highly reputable private histopathology lab located in central MA. We are looking for an experienced histologist with a strong background in routine and special stains, frozen sections and general histology procedures. Must be HT certified or eligible. Hours are somewhat flexible and salary is based on experience. We offer great benefits and a pleasant work environment. This is NOT a production line "slide factory". Please e-mail your cover letter, resume and salary requirements to info@masshistology.com. _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From MadaryJ <@t> MedImmune.com Tue Sep 14 10:32:10 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Tue Sep 14 10:32:16 2010 Subject: [Histonet] macroscopic demonstration of lung mets in rodent lung Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1302614C5D@MD1EV002.medimmune.com> Hi netters, I am thinking an acetic based fixative would be used to inlfate the mouse lung to make the metastatic portions stand out next to normal tissue.. An investigator wants to do a lung inflation with a fixative that will allow him to view the lung mets and count areas as he breadloafs the lung(even though it is small). Bouins or Davidsons maybe? He asks if India ink perfusion would work too. Anyone try that? Any feedback is appreciated. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From dchihc <@t> yahoo.com Tue Sep 14 10:36:41 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Sep 14 10:36:46 2010 Subject: [Histonet] Sakura X50 Microwave Tissue Processor In-Reply-To: <399458.78523.qm@web31804.mail.mud.yahoo.com> References: <399458.78523.qm@web31804.mail.mud.yahoo.com> Message-ID: <163449.64110.qm@web43504.mail.sp1.yahoo.com> Hi Carlos, ??We have been using the X50 for about a year now.?I noticed a huge improvement in microtomy and stainability immediately in some tissue types; skins, bloody specimens, gi biopsies?(which were somewhat troublesome at times with conventional processing).?Also,?there is no need for facing blocks in and rehydrating before sectioning (except decals...but a decal is a decal is a decal). ??Aside from the quality improvements, we were also able to eliminate a shift. Our?day shift begins at 6 am and ends at 5 pm. ? Any questions or?specific concerns you have, Im more than happy to help. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: Carlos Hernandez To: histonet@lists.utsouthwestern.edu Sent: Sat, September 11, 2010 10:57:11 PM Subject: [Histonet] Sakura X50 Microwave Tissue Processor I'm looking for some feedback (pros & cons) from anyone using the Sakura X50. Especially any derm labs that have experience with it. Thanks, Carlos ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Tue Sep 14 10:56:01 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Sep 14 10:56:07 2010 Subject: [Histonet] Histology stainers In-Reply-To: <356BA88EA30F44269DE017A15DB48CC3@DFS66DD1> References: <356BA88EA30F44269DE017A15DB48CC3@DFS66DD1> Message-ID: <111041.6153.qm@web43505.mail.sp1.yahoo.com> Hi Michelle, ? We retired our old Jung about 5 or 6 years ago and bought a Sakura Prisma with the linking tape coverslipper (they have them now with glass coverslips now I think). It has been great, unlike the older? coverslippers, this one doesn't use the conveyor belt so there are no slide pile-ups. This is?a complete walk away system. Cut slides, put them on and the stainer does the rest. You put them on, then take them off after they are coverslipped. We do some special stains on it as well as H&E, easy to program (touch screen)...And like the old Jung, the Prisma ?IS a workhorse. ? We retired our Excelsior Processor for The Sakura XPress50. The advantages of the Excelsior is the reagent rotation (just change out bottles of reagent and paraffin and the Excelsior rotates them). We switched to the XPress because of shift staffing problems and our desire to eliminate as much xylene usage as possible. We solved our staffing problems (eliminated the need for a second shift that was necessary with conventional processing), and now all the xylene we use is on the H&E stainer....HUGE reduction. ? We still use the old VIP for specimens that come in late that require longer fixation time. We put them on a delay program to come off the following day. ? ? ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: Michelle MacVeigh-Aloni To: histonet@lists.utsouthwestern.edu Sent: Thu, September 9, 2010 4:47:29 PM Subject: [Histonet] Histology stainers Hi histoneters, We have an old Jung Autostainer XL, which has been great, but it is very old and on its way out. What kind of newer stainers are you guys using? Should I even look at something diferent? I also would like to hear from the people using Exelsior tissue processor. Do you guys like it and how does it compare to the good old VIP? Michelle Aloni MS HTL ASCP Research Specialist USC Keck School of Medicine LA, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Tue Sep 14 11:53:43 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Sep 14 11:53:47 2010 Subject: [Histonet] PA Message-ID: <700714.26154.qm@web43511.mail.sp1.yahoo.com> Would the recruiter that has a PA in Ohio that wants to move South, please resend, the email to me with the info...thanks! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From CThornton <@t> dahlchase.com Tue Sep 14 12:53:04 2010 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue Sep 14 12:53:10 2010 Subject: [Histonet] poor collagen staining on skins Message-ID: Hello all, Our skin sections occasionally contain collagen that looks "burnt", to quote the pathologist. We use a Symphony stainer and Ventana has been here frequently trying to figure out the cause of the problem to no avail. Does anyone have any ideas? Could it be due to inadequate deparaffinization, inadequate baking, section thickness, slide issues, any of the above? We are just looking for some new ideas and fresh viewpoints! Thanks, Clare Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From maggie.allen <@t> nicewareintl.com Tue Sep 14 13:03:46 2010 From: maggie.allen <@t> nicewareintl.com (Maggie Allen) Date: Tue Sep 14 13:03:57 2010 Subject: [Histonet] Barcoding In-Reply-To: <20100909170227.D3ED4E8162@spamfilter2.redanvil.net> References: <20100909170227.D3ED4E8162@spamfilter2.redanvil.net> Message-ID: Our software (NiceLabel / NiceWatch) has the ability to take in data from any system. Data can be text, XML, HL7, JOB files, open formatted files, etc. We can connect via TCP/IP, Web Services, COM Port or a file drop. The data is them parsed out and place onto a label template formatted in NiceLabel Pro. A GUI design tool. We support all barcode symbologies (1D and 2D). We can then output to any printer that has a Windows print driver. Thermal, Laser, Line Matrix, Ink Jet or devices like slide and cassette printers. Please contact me if you would like additional information, a demonstration or trial of our software. Thank you, Maggie Allen Healthcare Business Development Manager Niceware International, LLC Tel (810) 629-3930 Cell (215) 200-0268 Email: maggie.allen@nicewareintl.com www.nicewareintl.com http://healthcare.nicewareintl.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, September 09, 2010 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 82, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. H&E+ Alcian Blue (Deborah Faichney) 2. RE: bar coding specimens, slides, blocks (Tom McNemar) 3. FW: [Histonet] Odd question (Morken, Tim) 4. RE: bar coding specimens, slides, blocks (Feher, Stephen) 5. RE: Pathology billing for consultation (Feher, Stephen) 6. Re: FW: [Histonet] Odd question (Kim.Donadio@bhcpns.org) 7. giving back tissue (Tench, Bill) 8. Re: bar coding specimens, slides, blocks (Langenberg, Stacey) 9. HT position in Colorado (Hale, Meredith) 10. tissue refixation ? (Benoit Loup) 11. Re: H&E+ Alcian Blue (Bryan Llewellyn) ---------------------------------------------------------------------- Message: 1 Date: Thu, 9 Sep 2010 09:44:39 +0100 From: Deborah Faichney Subject: [Histonet] H&E+ Alcian Blue To: "histonet@lists.utsouthwestern.edu" Message-ID: <8ED3F2CA5B78E142B8193376C57330F8EAE96449C5@EXCH2007.ad.stir.ac.uk> Content-Type: text/plain; charset="us-ascii" Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. ------------------------------ Message: 2 Date: Thu, 9 Sep 2010 06:04:06 -0400 From: Tom McNemar Subject: [Histonet] RE: bar coding specimens, slides, blocks To: "Horn, Hazel V" , "HISTONET@PATHOLOGY.SWMED.EDU" Message-ID: Content-Type: text/plain; charset="us-ascii" Also interested in this... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 3 Date: Thu, 9 Sep 2010 08:48:05 -0700 From: "Morken, Tim" Subject: FW: [Histonet] Odd question To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4C0255010115@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii This link will take you to the article about legalisms of tissue and blocks: http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12 Tim >>> "Morken, Tim" 9/8/2010 6:49 PM >>> There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org). It also addresses wet tissue, other things taken out of people. The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials." So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 3:18 PM To: 'Kim.Donadio@bhcpns.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Odd question Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 9 Sep 2010 12:03:55 -0400 From: "Feher, Stephen" Subject: RE: [Histonet] bar coding specimens, slides, blocks To: "Horn, Hazel V" , Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F461@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" We have had a great deal of success using Leica's IPC and IPS cassette and slide labelers. We use Soft Path but I had heard that the Leica LIS experts have experience interfacing with a number of LIS systems. We set these up basically as just another printer that is interfaced with our LIS system. The software within the Leica labelers produces the cassettes and slides with any configuration of accession number and/or bar code that you would like. We chose to use 2d barcode to save space on the cassette and slide. We also print our ThinPrep slides this way. Leica and Hologic got together to make sure we were able to use the correct coding to use these slides on our ThinPrep Imager. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 9 Sep 2010 12:06:21 -0400 From: "Feher, Stephen" Subject: RE: [Histonet] Pathology billing for consultation To: "Demarinis, Carolyn" , Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F462@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" There are a couple of ways to handle this and most of them have already been mentioned. We have a contract Pathology group in our hospital so if the patient insurance does not pay, the consultant bills the Pathology Group. Unless your pathologists are completely sold on their expert consultants, outside labs such as Genzyme will do the third party billing if you use their consultants. I know that they accept insurance assignment and have relationships with most large insurers so the patient rarely gets stuck with a bill. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 9:22 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 9 Sep 2010 11:12:55 -0500 From: Kim.Donadio@bhcpns.org Subject: Re: FW: [Histonet] Odd question To: "Morken, Tim" Cc: "histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Thank you all for responding. All of you were very helpful. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Morken, Tim" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2010 10:48 AM To "histonet@lists.utsouthwestern.edu" cc Subject FW: [Histonet] Odd question This link will take you to the article about legalisms of tissue and blocks: http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12 Tim >>> "Morken, Tim" 9/8/2010 6:49 PM >>> There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org). It also addresses wet tissue, other things taken out of people. The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials." So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 3:18 PM To: 'Kim.Donadio@bhcpns.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Odd question Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 9 Sep 2010 09:14:12 -0700 From: "Tench, Bill" Subject: [Histonet] giving back tissue To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A55D6@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii In all of this discussion, it is important to understand that there are significant variations in state laws that relate to this issue, so if this problem arises, have your hospital/lab attorney check into state laws very carefully. In California, the laboratory may not "own" the tissue (again, depending on releases never read but signed by the patient on admission) but it clearly is the responsible "custodian." This applies to sending out slides and blocks for research and for clinical testing. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 8 Date: Thu, 9 Sep 2010 10:16:11 -0600 From: "Langenberg, Stacey" Subject: Re: [Histonet] bar coding specimens, slides, blocks To: "Feher, Stephen" , "histonet-bounces@lists.utsouthwestern.edu" , "Horn, Hazel V" , "HISTONET@PATHOLOGY.SWMED.EDU" Message-ID: <99316908.1375278.1284048976798.JavaMail.rim@bda2340.bisx.prod.on.blackberry> Content-Type: text/plain; charset="iso-8859-1" We too use both the IPS and IPC. These are on such an open system we created our own LIS and now have barcoding from grossing to slide signout. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: "Feher, Stephen" Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Thu, 9 Sep 2010 10:03:55 To: Horn, Hazel V; HISTONET@PATHOLOGY.SWMED.EDU Subject: RE: [Histonet] bar coding specimens, slides, blocks We have had a great deal of success using Leica's IPC and IPS cassette and slide labelers. We use Soft Path but I had heard that the Leica LIS experts have experience interfacing with a number of LIS systems. We set these up basically as just another printer that is interfaced with our LIS system. The software within the Leica labelers produces the cassettes and slides with any configuration of accession number and/or bar code that you would like. We chose to use 2d barcode to save space on the cassette and slide. We also print our ThinPrep slides this way. Leica and Hologic got together to make sure we were able to use the correct coding to use these slides on our ThinPrep Imager. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 9 Sep 2010 11:21:24 -0500 From: "Hale, Meredith" Subject: [Histonet] HT position in Colorado To: Cc: "Roupp, Kevin" Message-ID: <6F33D8418806044682A391273399860F051F3FA5@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Colorado GI Pathology (CGIP) is seeking an experienced histotech to complement its existing staff. CGIP is a specialized lab located in Centennial, CO that provides services to the three largest gastroenterology practices in Denver. Dr. Russell Nash is the chief pathologist and medical director. Tracy Wrinkle is the Operations Manager. The position can be filled by an experienced person who can work from 32 to 40 hours per week. The schedule is basically days, although coverage for other times during vacations will be needed. The pay range starts at $26.00 per hour with benefits. This is a small, congenial work place with an emphasis on team work and cooperation. If you are interested in learning more about the opportunity, please contact Tracy Wrinkle at twrinkle@cogipath.com or call her at (303) 770-4848 for a confidential interview and tour of the facility. Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com ------------------------------ Message: 10 Date: Thu, 09 Sep 2010 18:23:04 +0200 From: Benoit Loup Subject: [Histonet] tissue refixation ? To: histonet@lists.utsouthwestern.edu Message-ID: <4C8909E8.7040800@jouy.inra.fr> Content-Type: text/plain; charset=windows-1252; format=flowed Hi to all, I have some troubles with H/E staining of tissues fixed in Bouin's. In fact it worked very well during my first assays and now my tissue sections are torn and looks like bad. I think that the fixation time was not sufficient (4h for 3mmx2mmx2mm pieces). Thus is it possible to dewax, rehydrate and refix tissue in bouin's before sectioning ? I also have some pieces already sectioned and mounted on slide but not yet dewaxed and stained. Is it also possible to refix these sections or are they definitively lost ? Thanks to all for your comments and help. Benoit -- Benoit Loup, PhD UMR Biologie du D?veloppement et Reproduction Diff?renciation des Gonades et Perturbations INRA ? Domaine de Vilvert B?timent Jacques Poly 78350 Jouy en Josas France Tel: 33 1 34 65 25 38 Fax: 33 1 34 65 22 41 E-mail: benoit.loup@jouy.inra.fr ------------------------------ Message: 11 Date: Thu, 9 Sep 2010 09:43:03 -0700 From: "Bryan Llewellyn" Subject: Re: [Histonet] H&E+ Alcian Blue To: "Histonet" Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original You are probably using a regressive hematoxylin. Most of those stain acid mucins purple-blue. To overcome it use a strictly progressive hematoxylin such as Mayer (hx 1 g., alum 50 g.) for 5 minutes. The order should be alcian blue, wash, H&E. Bryan Llewellyn ----- Original Message ----- From: "Deborah Faichney" To: Sent: Thursday, September 09, 2010 1:44 AM Subject: [Histonet] H&E+ Alcian Blue Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 82, Issue 11 **************************************** From trathborne <@t> somerset-healthcare.com Tue Sep 14 13:20:14 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Sep 14 13:20:20 2010 Subject: [Histonet] Dictation systems Message-ID: I know there has been some fairly recent discussion on Histonet about dictation systems, however I'm looking for users that we can perhaps visit to see how these systems work. The companies that we've contacted so far do not visit the lab. Rather, they do a web based presentation. The ones we've seen have been somewhat disappointing. I'm also interested in hearing what Cerner Millennium users are working with. If there are any dictation companies out there reading this, please contact me so we can discuss your product. Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From sfeher <@t> CMC-NH.ORG Tue Sep 14 17:28:31 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Tue Sep 14 17:28:36 2010 Subject: [Histonet] new lab design In-Reply-To: <72A45E102F6C43B3A3B3DF382A7D74F7@JimPC> References: <7722595275A4DD4FA225B92CDBF174A1E8D9568307@EXC-MBX3.cfs.le.ac.uk> <72A45E102F6C43B3A3B3DF382A7D74F7@JimPC> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F4D3@exchange.cmc-nh.org> You will want to make sure which equipment will require it's own dedicated circuit and make sure that's marked on the plans accordingly. One example is the ThinPrep Imager, which does require a dedicated circuit. If you have the luxury, I would suggest trying to get dedicated circuits for as many pieces of sensitive equipment as possible to avoid future issues as the equipment is upgraded. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Tuesday, September 14, 2010 8:24 AM To: 'Histonet' Subject: RE: [Histonet] new lab design Yes! We ran power strips along every bench-top _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Tuesday, September 14, 2010 4:24 AM To: 'Feher, Stephen'; Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design Just make sure you have many many many power sockets................. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: 13 September 2010 17:29 To: Blazek, Linda; Histonet Subject: RE: [Histonet] new lab design Hi Linda, We designed one from scratch without having a previous Path Lab in the hospital before. We are doing a workshop to that end at NSH in Seattle (WS 50). If you cannot attend the workshop, I will be happy to help in any that I can. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, September 13, 2010 8:10 AM To: 'Histonet' Subject: [Histonet] new lab design Good morning all. I am in the process of designing a new lab. We have grown beyond our walls and will be moving to a new building. If anyone has any great suggestions or ideas they would like to share I'd love your input! I'm still looking for a couple of tech too! Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Checked by AVG - www.avg.com Version: 9.0.851 / Virus Database: 271.1.1/3119 - Release Date: 09/13/10 14:35:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Sep 14 19:15:42 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Sep 14 19:31:03 2010 Subject: [Histonet] After hours autopsies Message-ID: <4C8FD7ED.7400.0077.1@harthosp.org> How many of you (especially Pathology Laboratories located in or that cover Children's Hospitals) do "after-hours" autopsies? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From AnthonyH <@t> chw.edu.au Tue Sep 14 19:43:14 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Sep 14 19:43:31 2010 Subject: [Histonet] After hours autopsies In-Reply-To: <4C8FD7ED.7400.0077.1@harthosp.org> Message-ID: Richard, We do not do out of hours autopsies (too costly). We will only collect samples for metabolic studies (muscle, liver, skin) out of hours (since they need to be collected asap post-mortem (and then you probably knew that!)). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, 15 September 2010 10:16 AM To: Histonet Subject: [Histonet] After hours autopsies How many of you (especially Pathology Laboratories located in or that cover Children's Hospitals) do "after-hours" autopsies? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From mucram11 <@t> comcast.net Tue Sep 14 21:19:52 2010 From: mucram11 <@t> comcast.net (mucram11@comcast.net) Date: Tue Sep 14 21:19:59 2010 Subject: [Histonet] After hours autopsies In-Reply-To: References: <4C8FD7ED.7400.0077.1@harthosp.org> Message-ID: <920058240-1284517194-cardhu_decombobulator_blackberry.rim.net-898360080-@bda385.bisx.prod.on.blackberry> We do not do after hours autopsies for either Children's or UAMS as a routine. This is not say we would not consider it under special circumstances. Specimens can be taken at either institute if ordered and required. Pam Marcum UAMS Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Tony Henwood" Sender: histonet-bounces@lists.utsouthwestern.edu Date: Wed, 15 Sep 2010 10:43:14 To: Richard Cartun; Histonet Subject: RE: [Histonet] After hours autopsies Richard, We do not do out of hours autopsies (too costly). We will only collect samples for metabolic studies (muscle, liver, skin) out of hours (since they need to be collected asap post-mortem (and then you probably knew that!)). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, 15 September 2010 10:16 AM To: Histonet Subject: [Histonet] After hours autopsies How many of you (especially Pathology Laboratories located in or that cover Children's Hospitals) do "after-hours" autopsies? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adesupo2002 <@t> hotmail.com Tue Sep 14 22:11:26 2010 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Tue Sep 14 22:11:30 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: References: , Message-ID: Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). From c_m_hernandezjr <@t> yahoo.com Tue Sep 14 23:15:39 2010 From: c_m_hernandezjr <@t> yahoo.com (c_m_hernandezjr@yahoo.com) Date: Tue Sep 14 23:15:58 2010 Subject: [Histonet] Re: X50 for Derm Lab In-Reply-To: References: Message-ID: <1962096948-1284524151-cardhu_decombobulator_blackberry.rim.net-152897653-@bda516.bisx.prod.on.blackberry> Melinda, Thank you so much for the very informative feedback regarding your processing. It helps a lot! Carlos Sent via BlackBerry from T-Mobile -----Original Message----- From: Melinda Sokol Date: Tue, 14 Sep 2010 18:25:14 To: Subject: X50 for Derm Lab Hi Carlos, My name is Melinda Hamilton and I work for a company called West Dermatology Pathology in Placentia CA. We hace 2 VIP K Processors (our back-up workhorses), a Leica Peloris and an X-50 from Sakura. Due to staffing, we use the X-50 and the Peloris as we can run continuously. For the X-50, we start a batch of 40 cassettes after grossing into a Pre-processing solution for 30 minutes, This includes shaves, punches, excisions and minute bx's. We then place the tissue into a molecular solution for 5 minutes, then onto the X-50 which then takes 1 1/2 hours. At the same time we can run a 2-hour process on the Peloris but minus the excisions. All excisions go on a 6- hour process if going onto the Peloris. By using the X-50 it cuts down on chemical waste, The Techs have an easier time of cutting the tissue and the Pathologists don't know the difference on what machine was used for processing. If you have any further questions, please fill free to call or e-mail back. Regards, Melinda A Hamilton HT (ASCP) NSH Tissue Control Curator LA Chapter President West Derm Pathology 1041 E Yorba Linda Blvd Placentia CA 92870 440-773-1783-cell m.sokol37@gmail.com From Ronald.Houston <@t> nationwidechildrens.org Wed Sep 15 07:41:33 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Sep 15 07:41:46 2010 Subject: [Histonet] After hours autopsies In-Reply-To: <4C8FD7ED.7400.0077.1@harthosp.org> References: <4C8FD7ED.7400.0077.1@harthosp.org> Message-ID: Richard, we do autopsies regularly at the weekends (both Saturday and Sunday) and also metabolic cases as soon as we get the permit. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, September 14, 2010 8:16 PM To: Histonet Subject: [Histonet] After hours autopsies How many of you (especially Pathology Laboratories located in or that cover Children's Hospitals) do "after-hours" autopsies? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From sbreeden <@t> nmda.nmsu.edu Wed Sep 15 07:54:29 2010 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Sep 15 07:57:45 2010 Subject: [Histonet] I'm in the New Lab! Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47391@nmdamailsvr.nmda.ad.nmsu.edu> We are physically moved and Organized Chaos prevails (except in Histo, of course!). There's been some discussion about what would be needed when designing a new lab and I agree with what has been offered - and actually used this sort of input from Histonet, along with the "lab design" course at NSH (which was priceless), and visiting a couple of labs before meeting with the architects. It was a long and arduous process but, with the exception of the chairs chosen by the majority in this building (about 700 people), was absolutely worth all the pain and agony. Please make note of our new physical address and a direct line to the histo lab (which I'll answer just as soon as I figure out how to work all those buttons and knobs!). Now to find out where I set up the microtome so I can start cutting! Good day, all! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From sbaldwin <@t> mhhcc.org Wed Sep 15 08:07:10 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Sep 15 08:08:56 2010 Subject: [Histonet] WS 50 Message-ID: HISTONETTERS I was trying my best to get out to Seattle for the workshop Steve was doing on the lab design, our budget wouldn't allow it but any help I can get would be great we are going to start construction Jan 2011 We are getting 3 patient rooms and making them one big cytology/histology lab. Is there a powerpoint or something out there to help me? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From HornHV <@t> archildrens.org Wed Sep 15 08:40:59 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Sep 15 08:41:04 2010 Subject: [Histonet] WS 50 In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB717F9A81E63@EVS1.archildrens.org> I would love this same information. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Wednesday, September 15, 2010 8:07 AM To: Histonet Subject: [Histonet] WS 50 HISTONETTERS I was trying my best to get out to Seattle for the workshop Steve was doing on the lab design, our budget wouldn't allow it but any help I can get would be great we are going to start construction Jan 2011 We are getting 3 patient rooms and making them one big cytology/histology lab. Is there a powerpoint or something out there to help me? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From KMB01 <@t> grh.org Wed Sep 15 08:50:45 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Wed Sep 15 08:50:51 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: Message-ID: I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dolores_Fischer <@t> baxter.com Wed Sep 15 10:52:22 2010 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Wed Sep 15 10:57:28 2010 Subject: [Histonet] C3 and C5b-9 Message-ID: Hi fellow histonetters, I am trying to work out an assay for complement 3 and C5b-9 detection in rodent. I have moved on to mouse, but have done some work with rats also. I haven't felt all that successful in my endeavors. In mice, the tissue of interest is kidney and have thus far proceeded in the following manner: Section frozen kidneys, place on + slides, air dry overnight, fix in chilled acetone 10 min, proceed with a standard ABC protocol. I have found rabbit poly from Abcam against C5b-9 in mouse and two anti-C3 antibodies from Thermo against mouse. My results haven't been all that great and the tissue often comes off the slides, so my results are ugly and frustrating. Also, I can only go by what I am told and that is that my kidneys should be expressing complement, so I don't really have a good control. Anyone out there familiar with C3/C5b-9 detection in rodents who can offer any advise or suggestions? We would like to stay away from fluorescent procedures if possible. Any thoughts as to why I might be losing my tissue off the slides? Thank you for your help, Dolores The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From MadaryJ <@t> MedImmune.com Wed Sep 15 11:12:29 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Sep 15 11:12:34 2010 Subject: [Histonet] vendors send me a quote for a slide and cassette etcher Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1302615311@MD1EV002.medimmune.com> Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From tkngflght <@t> yahoo.com Wed Sep 15 11:25:03 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Sep 15 11:25:06 2010 Subject: [Histonet] barcode systems Message-ID: <281801.91152.qm@web50907.mail.re2.yahoo.com> Hazel- ? Try calling Ventana.? They were just in my lab (large reference lab in Houston) and their evaluation system includes process and space evaluation and suggestions.? Barcoding speeds some areas and slows others down--nice to have them come in and show you were to save a lot of time so you get a fair trade for the extra effort for specimen integrity. ? I don't have his direct phone but the guy who came in was effective and our people related to him really well - Hylton Surrett was his name.? ? Cheryl Kerry, HT(ASCP) Houston TX From itai.moshe <@t> mail.huji.ac.il Wed Sep 15 11:27:29 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Wed Sep 15 11:27:38 2010 Subject: [Histonet] Re: Problem with liver fixation In-Reply-To: References: Message-ID: Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai From itai.moshe <@t> mail.huji.ac.il Wed Sep 15 11:34:51 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Wed Sep 15 11:34:55 2010 Subject: [Histonet] Masson's trichrom - problem with nuclei staining Message-ID: Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 With sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai P.S By mistake I've post this before in another message with a wrong title. please respond to that message, so the title will be o.k for future archive searching. From PMonfils <@t> Lifespan.org Wed Sep 15 11:35:49 2010 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Sep 15 11:36:27 2010 Subject: [Histonet] dimethyl methylene blue?? Message-ID: <4EBFF65383B74D49995298C4976D1D5E0727101D@LSRIEXCH1.lsmaster.lifespan.org> Hello, A researcher wants me to do "dimethyl methylene blue" staining on some cartilage sections. Is anyone familiar with this? Is it just another name for standard methylene blue staining? Or is it actually another dye? If so, where can I get the dye? And, does anyone have a staining protocol they are willing to share? Paul Monfils From billodonnell <@t> catholichealth.net Wed Sep 15 11:44:50 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Sep 15 11:45:06 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: References: Message-ID: I simply added the requirements for daily, weekly or monthly mainenance, temp recording, cleaning under the procedure of using each piece of equipment. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 8:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihiggins <@t> gmail.com Wed Sep 15 11:49:59 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Wed Sep 15 11:50:07 2010 Subject: [Histonet] filtering cytology stains Message-ID: Hello All, I was wondering how you are filtering your cytology stains. We are a relatively small lab, so we have been gravity filtering our cytology stains, but as we are getting busier with a larger volume of slides, especially fna's this is becoming a very time consuming process. I think vacuum filtration would be almost as lengthy a process. It was suggested that we look into getting some 200ml syringes that can come with an attached filter to suck in and pump out the fluid for faster filtration...is anyone using such a process? If so, do you have product names/numbers for the filters or the syringes. If anyone has another method they are using I would like to hear any suggestions. Thanks in advance for you input, Brandi Higgins, BS, HT(ASCP) From gentras <@t> auburn.edu Wed Sep 15 11:50:16 2010 From: gentras <@t> auburn.edu (Atoska Gentry) Date: Wed Sep 15 11:50:29 2010 Subject: [Histonet] RE: Adipose Cryosections Fixation Message-ID: <4C90B2F8.C676.0026.0@auburn.edu> hello! Will you either provide me with info & protocol for fixation of adipose cryosections prior to staining and/or contact info for Dr. M. Rocio Sierra-Honigmann ASAP? Thank you kindly, Atoska From CThornton <@t> dahlchase.com Wed Sep 15 12:15:41 2010 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Sep 15 12:15:47 2010 Subject: [Histonet] Ventana Symphony Message-ID: Hello all, I have some questions for other Symphony users out there. Can someone please contact me off-list if you don't mind answering some questions for me? Thanks much! Clare Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From patrick.lewis <@t> seattlechildrens.org Wed Sep 15 12:53:41 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Sep 15 12:53:52 2010 Subject: [Histonet] NBF fixation and long term storage Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C05AC2487@s107.childrens.sea.kids> I have some questions about Fixing tissues for paraffin embedding when the ultimate purpose will be looking for viral antigens and cell surface markers. We have some monkey tissue that has been in NBF for over a year. and we are just now processing them and embedding them in to paraffin. We will be getting additional tissues from monkey necropsies and in an effort to avoid epitope damage from prolonged exposure to NBF I was wondering if anyone could recommend a fixation period. (I.E. no longer than 24 hours in NBF, 48 hours? ETC. I was thinking of replacing the NBF (Neutral buffered Formalin) with 70% Etoh. And using 70% Etoh as long term storage of tissue parts. The ideal situation would be to get the tissues from necropsy the same day of the necropsy and store the tissues overnight in NBF and then transfer them to 70% etoh and then process them on the tissue processer after 24 hours in Etoh. I'm not sure how many animals I've eventually get and I'm a little concerned over where we will put all the spare animal parts that I'm sure I'll accumulate. We don't want to throw anything away and for some tissues I'll have more than I can put into a single cassette. I may end up using more than 1 cassette for a single piece of tissue so that I have backup cassettes of it. Anyone storing research tissues has a suggestion about the best way to store them when space is a premium. Thanks Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Wed Sep 15 12:58:46 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 15 12:58:50 2010 Subject: [Histonet] NBF fixation and long term storage In-Reply-To: <7EA5752B2903B143A5B845DEA87D5D1C05AC2487@s107.childrens.sea.kids> Message-ID: <135971.17925.qm@web65704.mail.ac4.yahoo.com> Patrick: Under separate cover I am sending an article about NBF fixation and its comparison with other fixatives so you can make an "informed" decision. Ren? J. --- On Wed, 9/15/10, Lewis, Patrick wrote: From: Lewis, Patrick Subject: [Histonet] NBF fixation and long term storage To: Histonet@lists.utsouthwestern.edu Date: Wednesday, September 15, 2010, 1:53 PM I have some questions about Fixing tissues for paraffin embedding when the ultimate purpose will be looking for viral antigens and cell surface markers. We have some monkey tissue that has been in NBF for over a year. and we are just now processing them and embedding them in to paraffin. We will be getting additional tissues from monkey necropsies and in an effort to avoid epitope damage from prolonged exposure to NBF I was wondering if anyone could recommend a fixation period.? (I.E. no longer than 24 hours in NBF, 48 hours? ETC. I was thinking of replacing the NBF (Neutral buffered Formalin) with 70% Etoh.? And using 70% Etoh as long term storage of tissue parts.? The ideal situation would be to get the tissues from necropsy the same day of the necropsy and store the tissues overnight in NBF and then transfer them to 70% etoh and then process them on the tissue processer after 24 hours in Etoh.? I'm not sure how many animals I've eventually get and I'm a little concerned over where we will put all the spare animal parts that I'm sure I'll accumulate. We don't want to throw anything away and for some tissues I'll have more than I can put into a single cassette. I may end up using more than 1 cassette for a single piece of tissue so that I have backup cassettes of it.? Anyone storing research tissues? has a suggestion about the best way to store them when space is a premium. Thanks Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115? OFFICE 000-000-0000? PAGER 000-000-0000? CELL 206-884-7311? FAX patrick.lewis@seattlechildrens.org OFFICE? 1900 9th Avenue Seattle, WA 98101 MAIL? ? ? M/S C9S-8, Seattle, WA 98101 WWW? ???seattlechildrens.org CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.? Any unauthorized review, use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Wed Sep 15 12:59:17 2010 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Sep 15 12:59:30 2010 Subject: [Histonet] Re: Problem with liver fixation Message-ID: <4C90B515020000A80004B3D7@ns.luhcares.org> Itai, Are you post fixing in Bouins? Post fix in heated Bouins for 1hr, wash well and then follow your protocal. Hope this helps, Matt Lunetta HT (ASCP) Longmont United Hospital Message: 2 Date: Wed, 15 Sep 2010 18:27:29 +0200 From: Itai Moshe Subject: [Histonet] Re: Problem with liver fixation To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai From Margaret.Perry <@t> sdstate.edu Wed Sep 15 13:53:26 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Sep 15 13:53:32 2010 Subject: [Histonet] drying ovens Message-ID: Has anyone used the TDO66 Medite drying oven? Do you like it? Is the company good to work with? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From sbaldwin <@t> mhhcc.org Wed Sep 15 14:20:42 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Sep 15 14:21:42 2010 Subject: [Histonet] ANP 11605 & 11610 Message-ID: HISTONETTERS I was wondering what some of your thoughts on this. Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From laurie.colbert <@t> huntingtonhospital.com Wed Sep 15 15:51:12 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Sep 15 15:51:17 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D7683098EFBB2@EXCHANGE3.huntingtonhospital.com> I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jackie.puma <@t> sterlingpath.com Wed Sep 15 17:45:53 2010 From: jackie.puma <@t> sterlingpath.com (J Puma) Date: Wed Sep 15 17:45:58 2010 Subject: [Histonet] Lab Supervisor/Manager Position in Orange County CA Message-ID: <878793.3958.qm@web1006.biz.mail.sp1.yahoo.com> ?Laboratory Supervisor /Manager preferably with CLS Qualification The Manager is a key role within the pathology organization located in Orange County California.? This individual will join our expanding laboratory facilities, and will participate in daily operations of Histology, IHC, Flow, Cytogenetic and Molecular Diagnostics. The candidate will report to the CEO/Medical Director. The ideal candidate must be have previous management experience with 5-10 years practical experience in the areas of Biopsy Histology, Cytology, Bone Marrow Morphology, Flow Cytometry, IHC experience, Molecular Genetics or relevant experience. ?CLS certified is preferred.We are looking for an exceptional professional who enjoys the challenge of working with a cross section of professionals in a out patient-focused environment.? Superior communication, collaboration, business and clinical judgment, emphasis on turnaround time, and organizational development skills are absolutely essential for success.? The ideal candidate will possess exceptional interpersonal, communication, and partnering skills that will allow effective interaction with employees at all levels, with laboratory professional peers throughout the company and with clients, client services and sales professionals.? Being persuasive and leading to consensus, and a commitment to the highest standards of excellence in the successful implementation of the best industry standards of the Medical Laboratory operations. From tliglesias <@t> ucdavis.edu Wed Sep 15 21:29:54 2010 From: tliglesias <@t> ucdavis.edu (Teresa Iglesias) Date: Wed Sep 15 21:29:59 2010 Subject: [Histonet] Re: transporting sectioned tissues In-Reply-To: References: Message-ID: Is there any way to relax curled brain sections? They've been in cryoprotectant and I tried sitting them in PBS for a while. They're to be used in an IHC- can they be rescued? > Thanks, > -- > Teresa Iglesias > Graduate Group in Animal Behavior > University of California-Davis > > From tliglesias <@t> ucdavis.edu Wed Sep 15 21:32:47 2010 From: tliglesias <@t> ucdavis.edu (Teresa Iglesias) Date: Wed Sep 15 21:32:51 2010 Subject: [Histonet] Help! Curled cryosections Message-ID: Is there any way to relax curled brain sections? They've been in > cryoprotectant and I tried sitting them in PBS for a while. They're to be > used in an IHC- can they be rescued? > > > >> Thanks, >> -- >> Teresa Iglesias >> Graduate Group in Animal Behavior >> University of California-Davis >> >> > > > > From cheastys <@t> svm.vetmed.wisc.edu Wed Sep 15 21:49:15 2010 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Wed Sep 15 21:49:21 2010 Subject: [Histonet] Leica-Surgipath EM Paraffin Issue Message-ID: Has anyone else noticed a change in the EM400 embedding medium from Surgipath? We have not changed anything in our embedding, but now the EM400 results in cracks in the blocks. Leica says although the labeling has changed to indicate the EM400 is now also for infiltrating, the paraffin has not changed. Thanks, Sandy SVM ~ UW Madison From tliglesias <@t> ucdavis.edu Wed Sep 15 22:52:30 2010 From: tliglesias <@t> ucdavis.edu (Teresa Iglesias) Date: Wed Sep 15 22:52:34 2010 Subject: [Histonet] Help! Curled cryosections In-Reply-To: <389CB0AD-FC9E-4664-8173-AEF32B4FF7D6@pbrc.edu> References: <389CB0AD-FC9E-4664-8173-AEF32B4FF7D6@pbrc.edu> Message-ID: Thanks, Montina The sections are 40um and I had no trouble with all my other brains (sectioned myself). I had help with these last two and they are all curled. I think they just got sectioned too fast or something. I'll try the shaker and see if it helps. Thanks! -Teresa On Wed, Sep 15, 2010 at 8:47 PM, Montina Van Meter < Montina.VanMeter@pbrc.edu> wrote: > Teresa, > I would put the sections through several 5-10 min. washes on a shaker > table. It is very important to make sure you have all of the cryoprotectant > rinsed out of the tissue or it will inhibit IHC staining. How thick are the > sections? Were they cut on a cryostat or freezing microtome? I routinely > cut rat brain at 40um and don't have any curling issues. That sometimes > occurs when the knife has come through the section of brain too rapidly. A > slow and steady motion is needed when cutting frozens. > > Good luck! > Tina Van Meter > > > Sent from my iPhone > > On Sep 15, 2010, at 9:37 PM, "Teresa Iglesias" > wrote: > > >> -- ______________________________ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis One Shields Avenue 2320 Storer Hall Davis, CA 95616 Office: 530-754-7837 Fax: 530-752-1449 tliglesias@ucdavis.edu ______________________________ From bstephen <@t> fastmail.fm Thu Sep 16 00:17:54 2010 From: bstephen <@t> fastmail.fm (Birgitta Stephenson) Date: Thu Sep 16 00:17:57 2010 Subject: [Histonet] Picrosirius Red Staining of mixed archaeological residues. [SEC=UNCLASSIFIED] Message-ID: <1284614274.17258.1395270577@webmail.messagingengine.com> Hello Histonetters, I have been using Picrosirius Red stain to detect collagen fibers in residues lifted from archaeological artefacts. The protocol has been adapted for staining slides rather than tissue samples however I am curious if it is normal that everything on the slide stains red. I realise that the collagen fibers can be easily detected in cross polarised and part polarised light ( yellow and orange = large fibers and green the thinner fibers and all are highly birefringent). While only the collagen changes colours in cross polarised light I am wondering if it is normal that everything on the slide picks up the stain to some extent and turns red (pink) when viewed in plane polarised light? This includes seed parts, plant lipids and ochre? Any thoughts or ideas? Thanks Regards Birgitta Stephenson Archaeological Microscopy Research Lab University of Queensland Birgitta Stephenson Pharmacist Advisor Veteran Affairs Pharmacy Advisory Centre Phone (07) 3223 8435 Fax (07) 3223 8651 ______________________________________________________________________ IMPORTANT 1. Before opening any attachments, please check for viruses. 2. This e-mail (including any attachments) may contain confidential information for the intended recipient. If you are not the intended recipient, please contact the sender and delete all copies of this email. 3. Any views expressed in this e-mail are those of the sender and are not a statement of Australian Government Policy unless otherwise stated. 4. Electronic addresses published in this email are not conspicuous publications and DVA does not consent to the receipt of commercial electronic messages. 5. To unsubscribe from emails from the Department of Veterans' Affairs (DVA) please go to http://www.dva.gov.au/contact_us/Pages/feedback.aspx , and advise which mailing list you would like to unsubscribe from. 6. Finally, please do not remove this notice. -- Birgitta Stephenson bstephen@fastmail.fm -- http://www.fastmail.fm - Or how I learned to stop worrying and love email again From louise.renton <@t> gmail.com Thu Sep 16 02:15:41 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Sep 16 02:15:46 2010 Subject: [Histonet] filtering cytology stains In-Reply-To: References: Message-ID: Hi - why not look at something like a millipore (millivex) syringe filter? This website has the application choice on it - or perhaps speak to your Millipore rep? http://www.millipore.com/techpublications/tech1/pb1951en00 On Wed, Sep 15, 2010 at 6:49 PM, Brandi Higgins wrote: > Hello All, > > I was wondering how you are filtering your cytology stains. We are a > relatively small lab, so we have been gravity filtering our cytology > stains, > but as we are getting busier with a larger volume of slides, especially > fna's this is becoming a very time consuming process. I think vacuum > filtration would be almost as lengthy a process. > It was suggested that we look into getting some 200ml syringes that can > come with an attached filter to suck in and pump out the fluid for faster > filtration...is anyone using such a process? If so, do you have product > names/numbers for the filters or the syringes. If anyone has another > method > they are using I would like to hear any suggestions. > > Thanks in advance for you input, > Brandi Higgins, BS, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From W.E.J.Hoekert <@t> olvg.nl Thu Sep 16 06:29:17 2010 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Thu Sep 16 06:29:24 2010 Subject: [Histonet] PMS2 References: Message-ID: <1190CB05C44B13409483514729C2FC360C0B0C@PAIT42.olvg.nl> We used the same antibody but then at 1:1200, retrieved with PH9 (PT module buffer 4, thermo) on a labvision stainer. We had beautiful results. Now, we switched to Ventana Benchmark XT (1:50, 30' cc1 + amplification) and our results are quite poor for PMS2: many cytoplasmatic staining. So if anybody has a good protocol for the Benchmark XT, I am very interested. Regards, Willem Hoekert OLVG, Amsterdam ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Dana Settembre Verzonden: wo 25-8-2010 12:12 Aan: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] PMS2 Diane, When I was doing PMS2 I was using BD's mouse antibody @ 1:10 Retreived with Dako's TRS in a steamer for 40 min, incubated the antibody for 60 minutes and I used a labelled polymer for the detection (Dako's Envision + Mouse) It was difficult to work up. Good Luck, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> 08/24/10 8:47 PM >>> Can anyone share their protocol for PMS2? I just keep getting background staining. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From trathborne <@t> somerset-healthcare.com Thu Sep 16 07:13:49 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Sep 16 07:13:56 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: <57BE698966D5C54EAE8612E8941D7683098EFBB2@EXCHANGE3.huntingtonhospital.com> Message-ID: If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From mhale <@t> carisls.com Thu Sep 16 08:41:08 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Thu Sep 16 08:41:18 2010 Subject: [Histonet] Great Position in Irving , Tx Message-ID: <6F33D8418806044682A391273399860F0532C861@s-irv-ex301.PathologyPartners.intranet> Nueterra, a leading developer and manager of physician-owned surgery centers and specialty hospitals has an immediate opportunity available for a Histotechnician. Great opportunity for a Histotechnician in a brand new laboratory! Nueterra Pathology Services in Irving, TX is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers a competitive salary, medical/dental insurance, 401K plan, and vacation/sick leave. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email at mhale@carisdx.com . Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From mhale <@t> carisls.com Thu Sep 16 08:42:07 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Thu Sep 16 08:42:18 2010 Subject: [Histonet] Great HT Position in Colorado Message-ID: <6F33D8418806044682A391273399860F0532C864@s-irv-ex301.PathologyPartners.intranet> Colorado GI Pathology (CGIP) is seeking an experienced histotech to complement its existing staff. CGIP is a specialized lab located in Centennial, CO that provides services to the three largest gastroenterology practices in Denver. Dr. Russell Nash is the chief pathologist and medical director. Tracy Wrinkle is the Operations Manager. The position can be filled by an experienced person who can work from 32 to 40 hours per week. The schedule is basically days, although coverage for other times during vacations will be needed. The pay range starts at $26.00 per hour with benefits. This is a small, congenial work place with an emphasis on team work and cooperation. If you are interested in learning more about the opportunity, please contact Tracy Wrinkle at twrinkle@cogipath.com or call her at (303) 770-4848 for a confidential interview and tour of the facility. Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From laurie.colbert <@t> huntingtonhospital.com Thu Sep 16 09:09:59 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Sep 16 09:10:08 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D7683098EFC2A@EXCHANGE3.huntingtonhospital.com> Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From kryan <@t> nfderm.com Thu Sep 16 09:23:17 2010 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Thu Sep 16 09:23:25 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: <57BE698966D5C54EAE8612E8941D7683098EFC2A@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683098EFC2A@EXCHANGE3.huntingtonhospital.com> Message-ID: How do you handle this if you have no one that comes in on the weekend? Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Sep 16 09:27:17 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Sep 16 09:27:21 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D7683098EFC32@EXCHANGE3.huntingtonhospital.com> I guess there's nothing you can do. -----Original Message----- From: Kaye Ryan [mailto:kryan@nfderm.com] Sent: Thursday, September 16, 2010 7:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Sep 16 09:31:33 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Sep 16 09:32:44 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: <57BE698966D5C54EAE8612E8941D7683098EFC32@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683098EFC32@EXCHANGE3.huntingtonhospital.com> Message-ID: By using a thermometer that records high and low temperatures, you can see if your fridge or freezer went out-of-range over the weekend. This satisfies CAP Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:27 AM To: Kaye Ryan; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I guess there's nothing you can do. -----Original Message----- From: Kaye Ryan [mailto:kryan@nfderm.com] Sent: Thursday, September 16, 2010 7:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From marktarango <@t> gmail.com Thu Sep 16 09:35:40 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Sep 16 09:35:46 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: References: <57BE698966D5C54EAE8612E8941D7683098EFBB2@EXCHANGE3.huntingtonhospital.com> Message-ID: Wouldn't those min/max thermometers help with this? So you would know if it went out of range during the weekend... Mark On Thu, Sep 16, 2010 at 5:13 AM, Rathborne, Toni < trathborne@somerset-healthcare.com> wrote: > If you do not have staff in on weekends/holidays, how do you monitor your > temperatures then? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie > Colbert > Sent: Wednesday, September 15, 2010 4:51 PM > To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ANP. 23075 > > > I interpret this to mean that I, as the supervisor, need to document > monthly that temps have been recorded and instrument maintenance has > been recorded. I review and initial temp and maintenance charts at the > end of each month to make sure that it has been done and that temps are > in range. > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. > Gorham > Sent: Wednesday, September 15, 2010 6:51 AM > To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ANP. 23075 > > I would like this information also please. > Kathy, Gorham, H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > Financial Excellence Award 2010 awarded by the national Rural Health > Research & Policy Analysis Center > Healthcare Achievement Award for Quality in Patient Care Delivery and > Satisfaction 2010 awarded by Amerinet > > GRH Mission > We will ensure access to high-quality, cost-effective health services in > a safe, customer-friendly environment for all those in need of our > services. > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s > only > and should be protected against viewing by unauthorized persons. The > information > herein may have been disclosed from records whose confidentiality is > protected > by Federal and State Law. Federal regulations prohibit further > distribution or > copying of this information without permission. If you received this > e-mail > transmission in error, please notify the sender immediately to arrange > for return > or destruction of this information. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO > ADESUYI > Sent: Tuesday, September 14, 2010 8:11 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ANP. 23075 > > > > > > > Hi, > Does anyone have a procedure on the CAP ANP. 23075 that they would > like to share? > Thanking you all for your usual cooperation. > > Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Ronald.Houston <@t> nationwidechildrens.org Thu Sep 16 09:36:43 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Sep 16 09:37:54 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: <57BE698966D5C54EAE8612E8941D7683098EFC32@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683098EFC32@EXCHANGE3.huntingtonhospital.com> Message-ID: By using a thermometer that records high and low temperatures, you can see if your fridge or freezer went out-of-range over the weekend. This satisfies CAP and, should have mentioned it earlier, JCAHO Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:27 AM To: Kaye Ryan; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I guess there's nothing you can do. -----Original Message----- From: Kaye Ryan [mailto:kryan@nfderm.com] Sent: Thursday, September 16, 2010 7:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Sep 16 09:54:23 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Sep 16 09:56:56 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: References: <57BE698966D5C54EAE8612E8941D7683098EFC2A@EXCHANGE3.huntingtonhospital.com>, Message-ID: We use a thermometer that will indicate to us if the temperature went high over the weekend. There is a little group of pellets at one end that will drop to the other end if the temperature is out of range. We call them Visual Indicator. You can either buy them through one of the major distributers. They come for freezers or refrigerators. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan [kryan@nfderm.com] Sent: Thursday, September 16, 2010 10:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Thu Sep 16 09:58:55 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Sep 16 09:59:03 2010 Subject: [Histonet] filtering cytology stains In-Reply-To: References: Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F4F2@exchange.cmc-nh.org> Hi Brandi, Depending on your stain line set up and the number of FNA's that you are doing, you may want to consider having multiple stain set up's that you can switch to in a hurry. This works well if you only filter stains and change alcohols. You can keep several boats or the smaller manual staining dishes filled with Hematoxlyn, OG and EA. Even better and quicker if you use the EA/OG combo stain. The stain dishes or boats that were used for positive FNA's can be tagged with a colored sticker and set aside to be filtered all together. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Thursday, September 16, 2010 3:16 AM To: Brandi Higgins; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] filtering cytology stains Hi - why not look at something like a millipore (millivex) syringe filter? This website has the application choice on it - or perhaps speak to your Millipore rep? http://www.millipore.com/techpublications/tech1/pb1951en00 On Wed, Sep 15, 2010 at 6:49 PM, Brandi Higgins wrote: > Hello All, > > I was wondering how you are filtering your cytology stains. We > are a relatively small lab, so we have been gravity filtering our > cytology stains, but as we are getting busier with a larger volume of > slides, especially fna's this is becoming a very time consuming > process. I think vacuum filtration would be almost as lengthy a > process. > It was suggested that we look into getting some 200ml syringes > that can come with an attached filter to suck in and pump out the > fluid for faster filtration...is anyone using such a process? If so, > do you have product names/numbers for the filters or the syringes. If > anyone has another method they are using I would like to hear any > suggestions. > > Thanks in advance for you input, > Brandi Higgins, BS, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Sep 16 10:03:03 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Sep 16 10:03:12 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D7683098EFC39@EXCHANGE3.huntingtonhospital.com> We use a continuous-read thermometer to monitor the temp in our block/slide storage area. They probably make these for refrigerators. -----Original Message----- From: McMahon, Loralee A [mailto:Loralee_Mcmahon@URMC.Rochester.edu] Sent: Thursday, September 16, 2010 7:54 AM To: Kaye Ryan; Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 We use a thermometer that will indicate to us if the temperature went high over the weekend. There is a little group of pellets at one end that will drop to the other end if the temperature is out of range. We call them Visual Indicator. You can either buy them through one of the major distributers. They come for freezers or refrigerators. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan [kryan@nfderm.com] Sent: Thursday, September 16, 2010 10:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Albert.Santiago <@t> uphs.upenn.edu Thu Sep 16 10:21:00 2010 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Thu Sep 16 10:22:09 2010 Subject: [Histonet] RE: Histonet Digest, Vol 82, Issue 20 In-Reply-To: <1477820941276883084190649434018@psmtp.com> References: <1477820941276883084190649434018@psmtp.com> Message-ID: Hello my friends in histonet world, I'd like to offer my insight into the weekend/holiday temp.(refrigerator) recording issue. I use a traceable thermometer (Fisher Scientific) that is very simple to use and very reliable. It records the daily temp. and the weekend min./max. temp. on Friday we press and hold the "memory clear" button for a few seconds and on Monday you can clearly see the daily temp. and the w/e min./max. temp. I hope this helps, any questions please feels free to contact me....albert.santiago@uphs.upenn.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, September 16, 2010 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 82, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Ventana Symphony (Clare Thornton) 2. NBF fixation and long term storage (Lewis, Patrick) 3. Re: NBF fixation and long term storage (Rene J Buesa) 4. Re: Problem with liver fixation (Matthew Lunetta) 5. drying ovens (Perry, Margaret) 6. ANP 11605 & 11610 (Sara Baldwin/mhhcc.org) 7. RE: ANP. 23075 (Laurie Colbert) 8. Lab Supervisor/Manager Position in Orange County CA (J Puma) 9. Re: transporting sectioned tissues (Teresa Iglesias) 10. Help! Curled cryosections (Teresa Iglesias) 11. Leica-Surgipath EM Paraffin Issue (Sandra Cheasty) 12. Re: Help! Curled cryosections (Teresa Iglesias) 13. Picrosirius Red Staining of mixed archaeological residues. [SEC=UNCLASSIFIED] (Birgitta Stephenson) 14. Re: filtering cytology stains (louise renton) 15. RE: PMS2 (Hoekert, W.E.J.) 16. RE: ANP. 23075 (Rathborne, Toni) 17. Great Position in Irving , Tx (Hale, Meredith) 18. Great HT Position in Colorado (Hale, Meredith) 19. RE: ANP. 23075 (Laurie Colbert) 20. RE: ANP. 23075 (Kaye Ryan) 21. RE: ANP. 23075 (Laurie Colbert) ---------------------------------------------------------------------- Message: 1 Date: Wed, 15 Sep 2010 13:15:41 -0400 From: Clare Thornton Subject: [Histonet] Ventana Symphony To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, I have some questions for other Symphony users out there. Can someone please contact me off-list if you don't mind answering some questions for me? Thanks much! Clare Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ------------------------------ Message: 2 Date: Wed, 15 Sep 2010 10:53:41 -0700 From: "Lewis, Patrick" Subject: [Histonet] NBF fixation and long term storage To: Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C05AC2487@s107.childrens.sea.kids> Content-Type: text/plain; charset="us-ascii" I have some questions about Fixing tissues for paraffin embedding when the ultimate purpose will be looking for viral antigens and cell surface markers. We have some monkey tissue that has been in NBF for over a year. and we are just now processing them and embedding them in to paraffin. We will be getting additional tissues from monkey necropsies and in an effort to avoid epitope damage from prolonged exposure to NBF I was wondering if anyone could recommend a fixation period. (I.E. no longer than 24 hours in NBF, 48 hours? ETC. I was thinking of replacing the NBF (Neutral buffered Formalin) with 70% Etoh. And using 70% Etoh as long term storage of tissue parts. The ideal situation would be to get the tissues from necropsy the same day of the necropsy and store the tissues overnight in NBF and then transfer them to 70% etoh and then process them on the tissue processer after 24 hours in Etoh. I'm not sure how many animals I've eventually get and I'm a little concerned over where we will put all the spare animal parts that I'm sure I'll accumulate. We don't want to throw anything away and for some tissues I'll have more than I can put into a single cassette. I may end up using more than 1 cassette for a single piece of tissue so that I have backup cassettes of it. Anyone storing research tissues has a suggestion about the best way to store them when space is a premium. Thanks Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 3 Date: Wed, 15 Sep 2010 10:58:46 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] NBF fixation and long term storage To: Histonet@lists.utsouthwestern.edu, PatrickLewis Message-ID: <135971.17925.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Patrick: Under separate cover I am sending an article about NBF fixation and its comparison with other fixatives so you can make an "informed" decision. Ren? J. --- On Wed, 9/15/10, Lewis, Patrick wrote: From: Lewis, Patrick Subject: [Histonet] NBF fixation and long term storage To: Histonet@lists.utsouthwestern.edu Date: Wednesday, September 15, 2010, 1:53 PM I have some questions about Fixing tissues for paraffin embedding when the ultimate purpose will be looking for viral antigens and cell surface markers. We have some monkey tissue that has been in NBF for over a year. and we are just now processing them and embedding them in to paraffin. We will be getting additional tissues from monkey necropsies and in an effort to avoid epitope damage from prolonged exposure to NBF I was wondering if anyone could recommend a fixation period.? (I.E. no longer than 24 hours in NBF, 48 hours? ETC. I was thinking of replacing the NBF (Neutral buffered Formalin) with 70% Etoh.? And using 70% Etoh as long term storage of tissue parts.? The ideal situation would be to get the tissues from necropsy the same day of the necropsy and store the tissues overnight in NBF and then transfer them to 70% etoh and then process them on the tissue processer after 24 hours in Etoh.? I'm not sure how many animals I've eventually get and I'm a little concerned over where we will put all the spare animal parts that I'm sure I'll accumulate. We don't want to throw anything away and for some tissues I'll have more than I can put into a single cassette. I may end up using more than 1 cassette for a single piece of tissue so that I have backup cassettes of it.? Anyone storing research tissues? has a suggestion about the best way to store them when space is a premium. Thanks Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115? OFFICE 000-000-0000? PAGER 000-000-0000? CELL 206-884-7311? FAX patrick.lewis@seattlechildrens.org OFFICE? 1900 9th Avenue Seattle, WA 98101 MAIL? ? ? M/S C9S-8, Seattle, WA 98101 WWW? ???seattlechildrens.org CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.? Any unauthorized review, use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 15 Sep 2010 11:59:17 -0600 From: "Matthew Lunetta" Subject: [Histonet] Re: Problem with liver fixation To: Message-ID: <4C90B515020000A80004B3D7@ns.luhcares.org> Content-Type: text/plain; charset=US-ASCII Itai, Are you post fixing in Bouins? Post fix in heated Bouins for 1hr, wash well and then follow your protocal. Hope this helps, Matt Lunetta HT (ASCP) Longmont United Hospital Message: 2 Date: Wed, 15 Sep 2010 18:27:29 +0200 From: Itai Moshe Subject: [Histonet] Re: Problem with liver fixation To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai ------------------------------ Message: 5 Date: Wed, 15 Sep 2010 13:53:26 -0500 From: "Perry, Margaret" Subject: [Histonet] drying ovens To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Has anyone used the TDO66 Medite drying oven? Do you like it? Is the company good to work with? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ------------------------------ Message: 6 Date: Wed, 15 Sep 2010 15:20:42 -0400 From: "Sara Baldwin/mhhcc.org" Subject: [Histonet] ANP 11605 & 11610 To: Message-ID: Content-Type: text/plain; charset=ISO-8859-1 HISTONETTERS I was wondering what some of your thoughts on this. Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ------------------------------ Message: 7 Date: Wed, 15 Sep 2010 13:51:12 -0700 From: "Laurie Colbert" Subject: RE: [Histonet] ANP. 23075 To: "Kathy M. Gorham" , "ADESUPO ADESUYI" , Message-ID: <57BE698966D5C54EAE8612E8941D7683098EFBB2@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 15 Sep 2010 15:45:53 -0700 (PDT) From: J Puma Subject: [Histonet] Lab Supervisor/Manager Position in Orange County CA To: histonet@lists.utsouthwestern.edu Message-ID: <878793.3958.qm@web1006.biz.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ?Laboratory Supervisor /Manager preferably with CLS Qualification The Manager is a key role within the pathology organization located in Orange County California.? This individual will join our expanding laboratory facilities, and will participate in daily operations of Histology, IHC, Flow, Cytogenetic and Molecular Diagnostics. The candidate will report to the CEO/Medical Director. The ideal candidate must be have previous management experience with 5-10 years practical experience in the areas of Biopsy Histology, Cytology, Bone Marrow Morphology, Flow Cytometry, IHC experience, Molecular Genetics or relevant experience. ?CLS certified is preferred.We are looking for an exceptional professional who enjoys the challenge of working with a cross section of professionals in a out patient-focused environment.? Superior communication, collaboration, business and clinical judgment, emphasis on turnaround time, and organizational development skills are absolutely essential for success.? The ideal candidate will possess exceptional interpersonal, communication, and partnering skills that will allow effective interaction with employees at all levels, with laboratory professional peers throughout the company and with clients, client services and sales professionals.? Being persuasive and leading to consensus, and a commitment to the highest standards of excellence in the successful implementation of the best industry standards of the Medical Laboratory operations. ------------------------------ Message: 9 Date: Wed, 15 Sep 2010 19:29:54 -0700 From: Teresa Iglesias Subject: [Histonet] Re: transporting sectioned tissues To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Is there any way to relax curled brain sections? They've been in cryoprotectant and I tried sitting them in PBS for a while. They're to be used in an IHC- can they be rescued? > Thanks, > -- > Teresa Iglesias > Graduate Group in Animal Behavior > University of California-Davis > > ------------------------------ Message: 10 Date: Wed, 15 Sep 2010 19:32:47 -0700 From: Teresa Iglesias Subject: [Histonet] Help! Curled cryosections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Is there any way to relax curled brain sections? They've been in > cryoprotectant and I tried sitting them in PBS for a while. They're to be > used in an IHC- can they be rescued? > > > >> Thanks, >> -- >> Teresa Iglesias >> Graduate Group in Animal Behavior >> University of California-Davis >> >> > > > > ------------------------------ Message: 11 Date: Wed, 15 Sep 2010 21:49:15 -0500 From: "Sandra Cheasty" Subject: [Histonet] Leica-Surgipath EM Paraffin Issue To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" Has anyone else noticed a change in the EM400 embedding medium from Surgipath? We have not changed anything in our embedding, but now the EM400 results in cracks in the blocks. Leica says although the labeling has changed to indicate the EM400 is now also for infiltrating, the paraffin has not changed. Thanks, Sandy SVM ~ UW Madison ------------------------------ Message: 12 Date: Wed, 15 Sep 2010 20:52:30 -0700 From: Teresa Iglesias Subject: Re: [Histonet] Help! Curled cryosections To: Montina Van Meter , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thanks, Montina The sections are 40um and I had no trouble with all my other brains (sectioned myself). I had help with these last two and they are all curled. I think they just got sectioned too fast or something. I'll try the shaker and see if it helps. Thanks! -Teresa On Wed, Sep 15, 2010 at 8:47 PM, Montina Van Meter < Montina.VanMeter@pbrc.edu> wrote: > Teresa, > I would put the sections through several 5-10 min. washes on a shaker > table. It is very important to make sure you have all of the cryoprotectant > rinsed out of the tissue or it will inhibit IHC staining. How thick are the > sections? Were they cut on a cryostat or freezing microtome? I routinely > cut rat brain at 40um and don't have any curling issues. That sometimes > occurs when the knife has come through the section of brain too rapidly. A > slow and steady motion is needed when cutting frozens. > > Good luck! > Tina Van Meter > > > Sent from my iPhone > > On Sep 15, 2010, at 9:37 PM, "Teresa Iglesias" > wrote: > > >> -- ______________________________ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis One Shields Avenue 2320 Storer Hall Davis, CA 95616 Office: 530-754-7837 Fax: 530-752-1449 tliglesias@ucdavis.edu ______________________________ ------------------------------ Message: 13 Date: Thu, 16 Sep 2010 15:17:54 +1000 From: "Birgitta Stephenson" Subject: [Histonet] Picrosirius Red Staining of mixed archaeological residues. [SEC=UNCLASSIFIED] To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <1284614274.17258.1395270577@webmail.messagingengine.com> Content-Type: text/plain; charset="utf-8" Hello Histonetters, I have been using Picrosirius Red stain to detect collagen fibers in residues lifted from archaeological artefacts. The protocol has been adapted for staining slides rather than tissue samples however I am curious if it is normal that everything on the slide stains red. I realise that the collagen fibers can be easily detected in cross polarised and part polarised light ( yellow and orange = large fibers and green the thinner fibers and all are highly birefringent). While only the collagen changes colours in cross polarised light I am wondering if it is normal that everything on the slide picks up the stain to some extent and turns red (pink) when viewed in plane polarised light? This includes seed parts, plant lipids and ochre? Any thoughts or ideas? Thanks Regards Birgitta Stephenson Archaeological Microscopy Research Lab University of Queensland Birgitta Stephenson Pharmacist Advisor Veteran Affairs Pharmacy Advisory Centre Phone (07) 3223 8435 Fax (07) 3223 8651 ______________________________________________________________________ IMPORTANT 1. Before opening any attachments, please check for viruses. 2. This e-mail (including any attachments) may contain confidential information for the intended recipient. If you are not the intended recipient, please contact the sender and delete all copies of this email. 3. Any views expressed in this e-mail are those of the sender and are not a statement of Australian Government Policy unless otherwise stated. 4. Electronic addresses published in this email are not conspicuous publications and DVA does not consent to the receipt of commercial electronic messages. 5. To unsubscribe from emails from the Department of Veterans' Affairs (DVA) please go to http://www.dva.gov.au/contact_us/Pages/feedback.aspx , and advise which mailing list you would like to unsubscribe from. 6. Finally, please do not remove this notice. -- Birgitta Stephenson bstephen@fastmail.fm -- http://www.fastmail.fm - Or how I learned to stop worrying and love email again ------------------------------ Message: 14 Date: Thu, 16 Sep 2010 09:15:41 +0200 From: louise renton Subject: Re: [Histonet] filtering cytology stains To: Brandi Higgins , Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi - why not look at something like a millipore (millivex) syringe filter? This website has the application choice on it - or perhaps speak to your Millipore rep? http://www.millipore.com/techpublications/tech1/pb1951en00 On Wed, Sep 15, 2010 at 6:49 PM, Brandi Higgins wrote: > Hello All, > > I was wondering how you are filtering your cytology stains. We are a > relatively small lab, so we have been gravity filtering our cytology > stains, > but as we are getting busier with a larger volume of slides, especially > fna's this is becoming a very time consuming process. I think vacuum > filtration would be almost as lengthy a process. > It was suggested that we look into getting some 200ml syringes that can > come with an attached filter to suck in and pump out the fluid for faster > filtration...is anyone using such a process? If so, do you have product > names/numbers for the filters or the syringes. If anyone has another > method > they are using I would like to hear any suggestions. > > Thanks in advance for you input, > Brandi Higgins, BS, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 15 Date: Thu, 16 Sep 2010 13:29:17 +0200 From: "Hoekert, W.E.J." Subject: RE: [Histonet] PMS2 To: "Dana Settembre" , , Message-ID: <1190CB05C44B13409483514729C2FC360C0B0C@PAIT42.olvg.nl> Content-Type: text/plain; charset="iso-8859-1" We used the same antibody but then at 1:1200, retrieved with PH9 (PT module buffer 4, thermo) on a labvision stainer. We had beautiful results. Now, we switched to Ventana Benchmark XT (1:50, 30' cc1 + amplification) and our results are quite poor for PMS2: many cytoplasmatic staining. So if anybody has a good protocol for the Benchmark XT, I am very interested. Regards, Willem Hoekert OLVG, Amsterdam ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Dana Settembre Verzonden: wo 25-8-2010 12:12 Aan: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] PMS2 Diane, When I was doing PMS2 I was using BD's mouse antibody @ 1:10 Retreived with Dako's TRS in a steamer for 40 min, incubated the antibody for 60 minutes and I used a labelled polymer for the detection (Dako's Envision + Mouse) It was difficult to work up. Good Luck, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> 08/24/10 8:47 PM >>> Can anyone share their protocol for PMS2? I just keep getting background staining. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ------------------------------ Message: 16 Date: Thu, 16 Sep 2010 08:13:49 -0400 From: "Rathborne, Toni" Subject: RE: [Histonet] ANP. 23075 To: "Laurie Colbert" , "Kathy M. Gorham" , "ADESUPO ADESUYI" , Message-ID: Content-Type: text/plain; charset="utf-8" If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 17 Date: Thu, 16 Sep 2010 08:41:08 -0500 From: "Hale, Meredith" Subject: [Histonet] Great Position in Irving , Tx To: Message-ID: <6F33D8418806044682A391273399860F0532C861@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Nueterra, a leading developer and manager of physician-owned surgery centers and specialty hospitals has an immediate opportunity available for a Histotechnician. Great opportunity for a Histotechnician in a brand new laboratory! Nueterra Pathology Services in Irving, TX is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers a competitive salary, medical/dental insurance, 401K plan, and vacation/sick leave. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email at mhale@carisdx.com . Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com ------------------------------ Message: 18 Date: Thu, 16 Sep 2010 08:42:07 -0500 From: "Hale, Meredith" Subject: [Histonet] Great HT Position in Colorado To: Message-ID: <6F33D8418806044682A391273399860F0532C864@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Colorado GI Pathology (CGIP) is seeking an experienced histotech to complement its existing staff. CGIP is a specialized lab located in Centennial, CO that provides services to the three largest gastroenterology practices in Denver. Dr. Russell Nash is the chief pathologist and medical director. Tracy Wrinkle is the Operations Manager. The position can be filled by an experienced person who can work from 32 to 40 hours per week. The schedule is basically days, although coverage for other times during vacations will be needed. The pay range starts at $26.00 per hour with benefits. This is a small, congenial work place with an emphasis on team work and cooperation. If you are interested in learning more about the opportunity, please contact Tracy Wrinkle at twrinkle@cogipath.com or call her at (303) 770-4848 for a confidential interview and tour of the facility. Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com ------------------------------ Message: 19 Date: Thu, 16 Sep 2010 07:09:59 -0700 From: "Laurie Colbert" Subject: RE: [Histonet] ANP. 23075 To: "Rathborne, Toni" , "Kathy M. Gorham" , "ADESUPO ADESUYI" , Message-ID: <57BE698966D5C54EAE8612E8941D7683098EFC2A@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 20 Date: Thu, 16 Sep 2010 10:23:17 -0400 From: "Kaye Ryan" Subject: RE: [Histonet] ANP. 23075 To: "Laurie Colbert" , "Rathborne, Toni" , "Kathy M. Gorham" , "ADESUPO ADESUYI" , Message-ID: Content-Type: text/plain; charset="us-ascii" How do you handle this if you have no one that comes in on the weekend? Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 16 Sep 2010 07:27:17 -0700 From: "Laurie Colbert" Subject: RE: [Histonet] ANP. 23075 To: "Kaye Ryan" , "Rathborne, Toni" , "Kathy M. Gorham" , "ADESUPO ADESUYI" , Message-ID: <57BE698966D5C54EAE8612E8941D7683098EFC32@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" I guess there's nothing you can do. -----Original Message----- From: Kaye Ryan [mailto:kryan@nfderm.com] Sent: Thursday, September 16, 2010 7:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 82, Issue 20 **************************************** The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From arsenn <@t> hsh.org Thu Sep 16 10:38:08 2010 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Thu Sep 16 10:38:14 2010 Subject: [Histonet] Cutting, Processing, etc Message-ID: Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. From Dolores_Fischer <@t> baxter.com Thu Sep 16 10:54:16 2010 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Thu Sep 16 10:55:06 2010 Subject: [Histonet] Losing frozen sections Message-ID: Hello again, Besides my C3 and C5b-9 question I was wondering if anyone has ideas as to why I might be losing my frozen sections during IHC. I'm placing 5u sections on charged slides, air drying overnight, fixing in chilled acetone, air drying again, and proceeding with the protocol, which recently has been an ABC method. Slides I don't use get stored in a -70 freezer. I do these by hand. Could I just be rinsing too vigorously? Sometimes I look at these during the procedure and they look fine, but inevitably many of the sections look horrible- torn and missing tissue, by the time they are coverslipped. Hints and suggestions anyone? Thank you in advance, Dolores The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From Timothy.Morken <@t> ucsfmedctr.org Thu Sep 16 10:58:36 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Sep 16 10:58:57 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: References: <57BE698966D5C54EAE8612E8941D7683098EFBB2@EXCHANGE3.huntingtonhospital.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C025501014F@EXMBMCB15.ucsfmedicalcenter.org> A cheap solution for the weekend is to get some electronic thermometers that record the high and low (Fisher has certified electronic thermometers pretty cheap). Then for the weekend you can document what the high and low was for that time period. Also, for critical reagents like antibodies, a chart recorder gives more information. And an alarm is helpful if it actually breaks down. There are temperature-monitoring software/hardware packages that you can put on your equipment that will record all instruments at once and, when connected to the internet, will call or email you if something goes out of range. They start as low a $1,500. You can also get temperature data loggers. They look like small batteries. You just put them in the place you want to log a temperature and they log the temp at intervals you program them for - like every minute, hour, whatever. Then you put them in a reader and download the info to a software program that allows you to make graphs. They are also great for logging temps when shipping items that are temperature sensitive. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From flnails <@t> texaschildrens.org Thu Sep 16 11:06:23 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Sep 16 11:06:49 2010 Subject: [Histonet] RE: Cutting, Processing, etc In-Reply-To: References: Message-ID: what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From cbarone <@t> NEMOURS.ORG Thu Sep 16 11:39:14 2010 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Thu Sep 16 11:39:18 2010 Subject: [Histonet] Region II Special Invitation Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A8003@wlmmsx01.nemours.org> YOU ARE INVITED!!! For those of you from Region II, who will be attending the NSH S/C in Seattle Sept 24 -30th. I would like to invite you to a special meeting for Virginia, W. Virginia, Washington, D.C.members and any others interested, from Region II...on re-establishing the By-laws for the Virgina Society to complete making it a fully active Society and electing officers for the BOD in VA. The meeting will be held on Saturday 5:00- 5:30 pm, directly following the Region II meeting at the convention Center. Please check your program for the room #. I look forward to seeing all of our Region members, and talking with you about establishing regional goals for the next two years. What is it that you need and what is it that we should be accomplishing? This is your region and you are in the driver seat...but, only if you attend and let me hear your voice. Lets set goals together! I would also like to invite members from our region to attend the NSH House of Delagate, to be held on Wednesday Sept 29th at 6pm (check program). Come and sit in the gallary to learn about how NSH works and how your ideas are incorporated in future plans for the Society. Always wonder what they Board of Drectors, Regional Directors and Delegates actually do? Come and sit in the gallary at the 36th NSH House of Delegate to see and learn....and truly hear NSH in Action! C. Barone, Region II Director From pruegg <@t> ihctech.net Thu Sep 16 11:54:57 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Sep 16 11:55:39 2010 Subject: [Histonet] Path Course Message-ID: <0FF5EC0AA0274EBA8427E4EDF87113B1@Patsyoffice> Dear colleagues, We just finalized the schedule for our fifth annual course. Many of you have already attended our previous courses. Feel free to forward this email to any interested colleagues. Also, exhibitors are welcome for sponsorship opportunities. We have a limited room this year, based on first come-first serve basis. All information about schedule, location, time, faculty, exhibitors prospectus, contact info, registration form, are available and downloadable online from the course's website at www.pathlearning.com Thanks, Hadi ================================ Hadi Yaziji, MD I Medical Director Vitro Molecular Laboratories I www.vitromolecular.com 7480 SW 40th Street, Suite 700 Miami, FL 33155 Tel 305 267 7979 I Fax 786 513 0175 Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From Rcartun <@t> harthosp.org Thu Sep 16 12:14:21 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Sep 16 12:14:31 2010 Subject: [Histonet] HSV mAb Message-ID: <4C92182D.7400.0077.1@harthosp.org> Is anyone using a commercially-available monoclonal antibody to Herpes Simplex Virus (for immunohistochemistry) that works well on formalin-fixed, paraffin-embedded tissue? I have not been happy with my polyclonal cocktail recently; too much nonspecific staining. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From plambert <@t> wisc.edu Thu Sep 16 12:21:48 2010 From: plambert <@t> wisc.edu (Paul Lambert) Date: Thu Sep 16 12:21:58 2010 Subject: [Histonet] Surgipath print labeler Message-ID: Does anyone have a used Surgipath print labeler for sale???? Thanks, Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 53706 USA tel: 608-262-8533 fax: 608-262-2824 email: plambert@wisc.edu From arsenn <@t> hsh.org Thu Sep 16 12:46:44 2010 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Thu Sep 16 12:46:50 2010 Subject: [Histonet] what is happening to our field? Message-ID: From: "Nails, Felton" Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii what is happening to our field?????????????? -----I agree! I was told it's a waste of time to change your blade and/or put your blocks in/on water before cutting. My mouth dropped to the ground. This is NOT what I was taught at school - and some of these procedures are killing me! I just want other professionals to confirm what I've already told them...thank you so much for all your private emails! Amy Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. From dchihc <@t> yahoo.com Thu Sep 16 13:26:05 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Thu Sep 16 13:26:10 2010 Subject: [Histonet] PA in Ohio Message-ID: <791055.92720.qm@web43503.mail.sp1.yahoo.com> Once again, would the recruiter for the PA in Ohio please contact me again. I forwarded your last email then deleted everything. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From dchihc <@t> yahoo.com Thu Sep 16 13:36:47 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Thu Sep 16 13:36:51 2010 Subject: [Histonet] Alabama Histology Position Message-ID: <708340.3684.qm@web43509.mail.sp1.yahoo.com> ________________________________ FINALLY another HistoTech position has been approved at DCH Regional Medical Center in Tuscaloosa, Alabama. We are a not for profit facility in West Alabama about 50 miles from Birmingham, about 4 hours from the Gulf of Mexico, and about 3 hours from Atlanta. We process about 15000 surgicals per year using Sakura VIP conventional processor, and Sakura ExPress 50 Rapid Tissue Processor, Sakura Prisma H&E Stainer with tape coverslipper and Ventana IHC automation. Hopefully we will be automated in Special Stains after October 1. Interested candidates must be proficient in embedding, microtomy, frozen sections, and (for now) manual special staining. Please contact Michelle Fagin at 205-759-7762 or Fax 205-750-5224 OR Sherrie Faulkner at 205-750-5736 or email mfagin@dchsystem.com Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From MW <@t> PersonifySearch.com Thu Sep 16 15:44:34 2010 From: MW <@t> PersonifySearch.com (Matthew Ward) Date: Thu Sep 16 15:44:42 2010 Subject: [Histonet] Outstanding Histotechnologist Position in FL!! Message-ID: <005401cb55df$f8fe1460$eafa3d20$@com> Good Afternoon Everyone, Our team at Personify has uncovered an extremely strong lab based Histotechnologist position in Leesburg FL. The position offers a very competitive salary and package. Please contact me directly to learn more. Have a great day!!! Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com lab based in Leesburg, FL TITLE: Histotechnologist II LOCATION: SUMMARY OF PURPOSE: Reports to the Histology Manager and is responsible for processing surgical specimens by performing various Histology procedures, including grossing, chemical fixation, embedding, microtomy, and staining of tissues and fluids. ESSENTIAL FUNCTIONS: 1. Receives and logs specimens into the Anatomic Pathology computer system. 2. Assess quality and appropriateness of specimens received and takes appropriate action if acceptable standard is not met. 3. Performs embedding, microtomy and staining of Histology and Cytology Specimens to meet established productivity and quality standards. 4. Perform gross description on small biopsies. 5. Maintains neat, organized and legible written records 6. Maintains a clean organized work area 7. Maintains appropriate supply inventory at assigned area. Performs supply inventory. 8. Performs routine and special Histology staining procedures accurately and assists in recognition and resolution of potential problems. 9. Files a variety of Histology materials, including but not limited to slides and blocks. 10. Retains a variety of materials including slides, blocks, specimens, requisitions and reports for the appropriate amount of time to comply with inspecting agencies. 11. Follows specialized staining procedures. 12. Participates in in-service training as required. 13. Participates in continuing education as required. 14. Performs Cytology processing as necessary. 15. Prioritizes and reorganizes workload in response to emergency procedures. 16. Adheres to departmental policies and procedures. 17. Demonstrates flexibility in work schedule to include weekends and holidays. 18. Performs required maintenance on all Histology equipment. 19. Documents special stain Q.C. and notifies a level II or III Histotech when Q.C. parameters are not met. 20. Demonstrate professionalism as evidenced by the cooperative attitude of peers and associated staff. 21. Performs minor Histology procedures in conjunction with other Histology personnel such as specimen registration, specimen disposal, filing slides and completing appropriate paperwork for consults. 22. Demonstrates commitment to our mission and Customer Service by adhering to all facets of the Laboratory policies and procedures. 23. Performs other duties as assigned. POSITION REQUIREMENTS: Education and formal training: AS Degree required License: Certification by the Board of Registry of American Society of Clinical Pathologists (ASCP) required. Experience: At least 1 year work experience performing the essential functions of a Histotechnologist. Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From plambert <@t> wisc.edu Thu Sep 16 16:04:10 2010 From: plambert <@t> wisc.edu (Paul Lambert) Date: Thu Sep 16 16:04:29 2010 Subject: [Histonet] clarification; slide printer Message-ID: <87A8D541-039B-4410-A340-DD1766A121A2@wisc.edu> I am looking for a used Surgipath slide printer to buy. Thanks, Paul F. Lambert, Ph.D. Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 53706 USA tel: 608-262-8533 fax: 608-262-2824 email: plambert@wisc.edu From sfeher <@t> CMC-NH.ORG Thu Sep 16 16:28:01 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Sep 16 16:28:06 2010 Subject: [Histonet] Formalin Incidental Spill vs a Release Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F512@exchange.cmc-nh.org> I trying to see if there is an industry standard for pathology labs regarding formalin spills. Can you tell me what volume of a formalin spill is considered an incidental spill that can be cleaned up by staff versus the volume of formalin that would constitute a "Release" requiring a Haz Mat team or a responder (such as a fire department) from outside of the hospital or lab to clean it up. I will appreciate seeing how this is handled in your institution. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From catherinesimonson <@t> gmail.com Thu Sep 16 17:01:16 2010 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Thu Sep 16 17:01:20 2010 Subject: [Histonet] Cutting, Processing, etc In-Reply-To: References: Message-ID: Well, to answer you questions in order base on my experience in most of the labs I have worked in, except one: 1) after embedding we face in all our blocks. as we face them in, we place the blocks on a pan of melting ice to a) chill them and b) allow the tissue to absorb a bit of moisture. all tissues are treated the same, keeping in mind that some tissue may need a little longer to soak on ice pan than others, but not much. 2) a new blade is always used for taking the "keeper" sections. 3) except for one lab, "freezy spray" has never been used. too easy to over chill the block and cause freezing artifacts that way. which, oddly enough, does not happen on melting ice. 4) even though you did not ask, I will also say, that the slides when cut are placed standing up for a few minutes to properly drain before applying any heat. if the slides are not allowed to drain before placing in the oven and stained (this goes for routine H&E as well as immuno) the sections will not adhere to the slides properly and will be prone to floating off/fragmenting during staining. It should also be noted that if all slides are allowed to dry thourouly before heat and staining there is little to no need for adding adhesive to the water bath. Hope this helps. Catherine On Thu, Sep 16, 2010 at 11:38 AM, Senn, Amy R wrote: > Hello Histoland! > > > > I have some questions about procedures in different histo labs and I'd > like to have some 'backup' when people look at me like I'm crazy here... > > > > After embedding, you face (trim) your blocks, right? Do you take > sections right from that same blade, or move/change your blade? > > How many of you 'soak' your blocks in water/softblock before cutting > them? Do you put them on a cold plate/use ice and water, etc? Does this > depend on the type of tissue, or do you treat them all the same? > > > > How often is "freezy" spray used in your lab, and where and when do you > use it? > > > > How often do you rotate/change your reagents in your processors? Do you > calculate this by how many blocks/days/weeks of use? > > > > Thank you so much for your input!! > > > > Amy Senn, HT > > Holy Spirit Hospital, Camp Hill PA > > > > > > > > > > > > > > > > > > > > > > Attention: This Message is intended only for the use of the individual or > entity to which it is addressed, and may contain information that is > privileged, confidential and exempt from disclosure under applicable law. If > the reader of this message is not the intended recipient, you are hereby > notified that any dissemination or copying of this message or the taking of > any action in reliance on the contents of this message is strictly > prohibited. If you have received this message in error, please notify us > immediately and destroy the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CIngles <@t> uwhealth.org Thu Sep 16 17:42:01 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Sep 16 17:43:01 2010 Subject: [Histonet] RE: Cutting, Processing, etc References: Message-ID: DON'T get me started... And I have only been practicing 9 years! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Nails, Felton Sent: Thu 9/16/2010 11:06 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cutting, Processing, etc what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! From DianaRip1 <@t> aol.com Thu Sep 16 19:45:00 2010 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Thu Sep 16 19:45:21 2010 Subject: [Histonet] Cassette Labeler Message-ID: <1f824.1e3bf86f.39c4140c@aol.com> I work in a small lab and process approx 150 cassettes a day. We currently use a chemical resistant pen that works great. We write the number on top and the patient initials on the side. We are considering getting a cassette labeler. Is it really worth the expense for 150 blocks a day? Is it possible to enter information on the side of the cassette? Can you share some of your experience with different ones with me. From je2 <@t> sanger.ac.uk Fri Sep 17 02:57:02 2010 From: je2 <@t> sanger.ac.uk (Jeanne Estabel) Date: Fri Sep 17 02:57:12 2010 Subject: [Histonet] Path Course References: <0FF5EC0AA0274EBA8427E4EDF87113B1@Patsyoffice> Message-ID: <34627BB49F43DC43A4A7D90B1B6F739D6782CF@exchsrv4.internal.sanger.ac.uk> Hi, This looks really interesting. Do you know if there is the same type of path courses/workshop for rodents pathology? Regards Jeanne Jeanne Estabel, PhD Scientific Manager MGP Histology Operations Manager Wellcome Trust Sanger Institute Cambridge UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: 16 September 2010 17:55 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Path Course Dear colleagues, We just finalized the schedule for our fifth annual course. Many of you have already attended our previous courses. Feel free to forward this email to any interested colleagues. Also, exhibitors are welcome for sponsorship opportunities. We have a limited room this year, based on first come-first serve basis. All information about schedule, location, time, faculty, exhibitors prospectus, contact info, registration form, are available and downloadable online from the course's website at www.pathlearning.com Thanks, Hadi ================================ Hadi Yaziji, MD I Medical Director Vitro Molecular Laboratories I www.vitromolecular.com 7480 SW 40th Street, Suite 700 Miami, FL 33155 Tel 305 267 7979 I Fax 786 513 0175 Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE. From k84as <@t> yahoo.com Fri Sep 17 04:37:39 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Sep 17 04:37:45 2010 Subject: [Histonet] RE: Cutting, Processing, etc In-Reply-To: Message-ID: <916570.85095.qm@web112601.mail.gq1.yahoo.com> i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention:? This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abijag76 <@t> rediffmail.com Fri Sep 17 03:39:40 2010 From: abijag76 <@t> rediffmail.com (abijag ) Date: Fri Sep 17 04:40:56 2010 Subject: [Histonet] Rat heart valve histology trimming and sectioning reg Message-ID: <20100917083940.55478.qmail@f6mail-145-154.rediffmail.com> Dear Histonetters, Our pathologists are interested in rat heart valvular lesions. I am working on trimming and sectioning methods to get uniform and reproducible morphology of heart valves in paraffin sections. It seems that available literature dealing with this is limited. Requesting your valuable experience in this regard. Thanks a lot Abi jagannath From Sandra.Harrison3 <@t> va.gov Fri Sep 17 08:32:40 2010 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Sep 17 08:32:59 2010 Subject: [Histonet] Cassette Labeler In-Reply-To: <1f824.1e3bf86f.39c4140c@aol.com> References: <1f824.1e3bf86f.39c4140c@aol.com> Message-ID: We have the Leica Cassette Labeler. It works just fine. I have looked at the Thermo Fisher Cassette Printer, which had a much smaller footprint than the Leica. I would be tempted to go with the Thermo if I have to replace my current labeler. These are expensive instruments but worth having. It would be appropriate for your workload. Plus, these cassette labelers can print a bar code, in addition to the pt. id and name. Laboratories can eliminate numbering errors by utilizing bar code scanners throughout the entire work flow; at grossing, processing and microtomy. Bar coding can also assist you with implementing LEAN processes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DianaRip1@aol.com Sent: Thursday, September 16, 2010 7:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler I work in a small lab and process approx 150 cassettes a day. We currently use a chemical resistant pen that works great. We write the number on top and the patient initials on the side. We are considering getting a cassette labeler. Is it really worth the expense for 150 blocks a day? Is it possible to enter information on the side of the cassette? Can you share some of your experience with different ones with me. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Fri Sep 17 08:36:50 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Sep 17 08:36:54 2010 Subject: [Histonet] Cassette Labeler In-Reply-To: <1f824.1e3bf86f.39c4140c@aol.com> References: <1f824.1e3bf86f.39c4140c@aol.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F521@exchange.cmc-nh.org> Thermo has a relatively low cost labeler for smaller quantities of cassette printing. The cost of the labeler versus the cost and potential for errors (hand writing) needs to be considered when making this kind of decision. You might want to do a study where you actually measure the time it takes to label one cassette. Make sure you start the clock from the time the tech actually prepares to label the cassette until they are finished and place the cassette down. Multiply that times the number of cassettes. Take that answer and multiply it times the tech salary plus benefits and you get the actual cost of hand labeling the cassettes. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DianaRip1@aol.com Sent: Thursday, September 16, 2010 8:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler I work in a small lab and process approx 150 cassettes a day. We currently use a chemical resistant pen that works great. We write the number on top and the patient initials on the side. We are considering getting a cassette labeler. Is it really worth the expense for 150 blocks a day? Is it possible to enter information on the side of the cassette? Can you share some of your experience with different ones with me. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Fri Sep 17 08:37:11 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Sep 17 08:37:18 2010 Subject: [Histonet] Cassette Labeler In-Reply-To: <1f824.1e3bf86f.39c4140c@aol.com> References: <1f824.1e3bf86f.39c4140c@aol.com> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA139309A022@NADCWPMSGCMS03.hca.corpad.net> Good Morning, We process about that many blocks per day also, and I would say YES it is worth getting a cassette labeler. Leica/Surgipath has a small semi-automatic cassette labeler that we are in the process of buying and we LOVE it. It will print the accession# and the patient's name on the front of the cassette which complies with CAP's 2 pt identifiers. The automated part is you can set it to advance to the next acc # or advance the block or specimen numbers. Tonia Crook is our rep and her cell # is 803-237-9969 or call your Leica rep in your area. Happy cutting!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DianaRip1@aol.com Sent: Thursday, September 16, 2010 8:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler I work in a small lab and process approx 150 cassettes a day. We currently use a chemical resistant pen that works great. We write the number on top and the patient initials on the side. We are considering getting a cassette labeler. Is it really worth the expense for 150 blocks a day? Is it possible to enter information on the side of the cassette? Can you share some of your experience with different ones with me. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nyilmaz <@t> mersin.edu.tr Fri Sep 17 08:41:14 2010 From: nyilmaz <@t> mersin.edu.tr (Nejat Yilmaz) Date: Fri Sep 17 09:18:47 2010 Subject: [Histonet] caspase 3 ihc on rabbit tissue Message-ID: <001201cb566e$09011bd0$6601640a@nejat1> Dear Friends, We are planning to perform active caspase 3 immunohistochemistry on FFPE rabbit cornea tissues. Most antibodies I have found are not working on rabbit. Any suggestion??? Thanks in advance... Dr. Necat Yilmaz Mersin University TURKEY __________ ESET NOD32 Antivirus Ak?ll? G?venlik taraf?ndan sa?lanan bilgiler, vir?s imza veritaban? s?r?m?: 5458 (20100917) __________ ?leti ESET NOD32 Antivirus Ak?ll? G?venlik taraf?ndan denetlendi. http://www.nod32.com.tr From alyssa <@t> alliedsearchpartners.com Fri Sep 17 09:22:59 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Sep 17 09:23:03 2010 Subject: [Histonet] Position in OH for 6-8 week coverage Message-ID: Allied Search Partners has been retained to search for a Histotechnician or Histotechnologist who can remit their services to our client for 6-8 week coverage. The physician is looking for a qualified candidate to work ASAP. *Location of Laboratory:* Toledo, OH (local candidates please) *Environment:* Multi-specialty clinic serving the community with 108 physicians and 37 specialties. *Shift:* Full Time, Monday-Friday from 8am-5pm *Department:* Dermasurgery *Requirements: * HT ASCP Prior MOHS experienced preferred CLIA eligible to gross *Job Duties:* Grossing Assist physician Make permanent sections MOHS Histology Other related duties *Other:* * * This is not a temporary contract assignment. This is a 6-8 week coverage. You will be paid by the employer (our client), *so please submit your salary requirements with your resume* to be considered for this position. For more information and to schedule a phone interview with one of our recruiters please submit resume to Alyssa@alliedsearchpartners.com -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From amario3 <@t> uic.edu Fri Sep 17 09:31:16 2010 From: amario3 <@t> uic.edu (Andrea Marion) Date: Fri Sep 17 09:31:21 2010 Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining Message-ID: <3363065ee4098fba6e4ee9ad0804e9f8.squirrel@webmail.uic.edu> Hi Itai, I am interested to hear if you've resolved this problem. We use the same kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is similar to the one you mentioned. I cannot get nuclei staining with this method either. The nuclei are well stained (ie black) up to the PMA/PTA step, but during that step the nuclear stain is completely removed. I cannot shorten the PMA/PTA step without negatively effecting the collagen stain. I have in our original protocol that the Weigert's working solution is good for one month, but I cannot recall if this is from Sigma's specification sheet or a personal observation from a lab member. However, from reading online some say it is good up to 4 months, others say it needs to be prepared fresh each time. I have tried fresh preparations with the same results. My instinct is that something is off - the staining is just not stable enough to withstand the subsequent acid steps in Masson's trichrome. Can an expert weigh in on this? Is there a way to strengthen nuclear staining from Weigert's? Sigma's formulas and usage recommendations are: Part A: 1% w/v certified Hematoxylin in ethanol Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid Combine equal volumes Part A and Part B, stain sections for 5 minutes. Andrea Marion Graduate Student University of Illinois at Chicago amario3 /at/ uic /dot/ edu Itai Moshe itai.moshe <@t> mail.huji.ac.il Wed Sep 15 11:34:51 CDT 2010 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 With sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai P.S By mistake I've post this before in another message with a wrong title. please respond to that message, so the title will be o.k for future archive searching. From deannal78 <@t> verizon.net Fri Sep 17 10:08:11 2010 From: deannal78 <@t> verizon.net (Deanna Rhoads) Date: Fri Sep 17 10:08:15 2010 Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining In-Reply-To: <3363065ee4098fba6e4ee9ad0804e9f8.squirrel@webmail.uic.edu> References: <3363065ee4098fba6e4ee9ad0804e9f8.squirrel@webmail.uic.edu> Message-ID: <528158.30084.qm@web84308.mail.re1.yahoo.com> I do Weigert's staining for 10 minutes and use it for a week at the most.? I, too, have heard conflicting times about the stability of the Weigert's, but have the best results with using it no longer than a week. Deanna Rhoads HT (ASCP) ________________________________ From: Andrea Marion To: histonet@lists.utsouthwestern.edu Cc: itai.moshe@mail.huji.ac.il Sent: Fri, September 17, 2010 10:31:16 AM Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining Hi Itai, I am interested to hear if you've resolved this problem.? We use the same kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is similar to the one you mentioned. I cannot get nuclei staining with this method either. The nuclei are well stained (ie black) up to the PMA/PTA step, but during that step the nuclear stain is completely removed. I cannot shorten the PMA/PTA step without negatively effecting the collagen stain. I have in our original protocol that the Weigert's working solution is good for one month, but I cannot recall if this is from Sigma's specification sheet or a personal observation from a lab member.? However, from reading online some say it is good up to 4 months, others say it needs to be prepared fresh each time. I have tried fresh preparations with the same results. My instinct is that something is off - the staining is just not stable enough to withstand the subsequent acid steps in Masson's trichrome. Can an expert weigh in on this? Is there a way to strengthen nuclear staining from Weigert's? Sigma's formulas and usage recommendations are: Part A: 1% w/v certified Hematoxylin in ethanol Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid Combine equal volumes Part A and Part B, stain sections for 5 minutes. Andrea Marion Graduate Student University of Illinois at Chicago amario3 /at/ uic /dot/ edu Itai Moshe itai.moshe <@t> mail.huji.ac.il Wed Sep 15 11:34:51 CDT 2010 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 With sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a? jar with simple tap water for a few minutes ? Thank's Itai P.S By mistake I've post this before in another message with a wrong title. please respond to that message, so the title will be o.k for future archive searching. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Sep 17 11:40:13 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Sep 17 11:40:32 2010 Subject: [Histonet] Re: Poor Weigerts Hematoxylin staining with Massons Trichrome Message-ID: <000901cb5687$0240b580$06c22080$@callis@bresnan.net> In general, we found the original/classic Weigerts iron hematoxylin stain to be weak and almost washed out of tissues after staining with Massons Trichrome. We no longer use the original formula, but a more concentrated modified formulation that is not differentiated out as much by the acidifed solutions found in Mass Tri. We also found this problem with Massons Trichrome kit components, where companies probably package the original method's solutions. We make up Weigerts fresh each time, and if it will last for a week for you, fine......... but our work tends to be a one time staining with Mass Tri, then weeks before it was done again. The problem is: ferric chloride continues to oxidize the hematoxylin throughout over time, that week or longer, weakening the iron hematoxylin staining capabililty. This is discussed in Sheehan and Hrapchak Theory and Practice of Histotechnology. Acid decalcified bone presents even more of a challenge, since nuclei (DNA/RNA) in cells are hydrolyzed by acid decalcifiers, compromising staining of nuclei, a problem seen with routine H&E staining. Deanna was correct on her assessment of this stain for best results. This modified Weigerts Hematoxylin was published in J of Histotechnology in a paper on Kreybergs stain on skin. The second Extra Strength Weigerts was found on Histonet years ago and I have not tried the latter. I suggest you see which one you prefer, as we use the first Modified Weigerts. Over the years, the modified gave us far superior nuclear staining with Massons Trichrome. Weigert's Iron Hematoxyin MODIFIED (found in J of Histotechnology in a method for Kreybergs stain on mouse skin). Solution A. 2% Hematoxylin in 95% ethanol Solution B. 62% Ferric Chloride 4 ml Distilled water 95 ml Hydrochloric acid 1 ml Mix equal amounts of Solution A and Solution B MIX FRESH JUST BEFORE USE AND DISCARD AFTER USE. Extra Strength Weigerts Iron Hematoxylin from Histonet (reference unknown, but supposedly from J of Histotechnology and method originated by Mabel Myli, Mayo Clinic) Solution A: Hematoxylin 10 g 95% ethanol 100 ml Solution B: 11.6 g Ferric Chloride 980 ml Distilled water 10 ml 25% hydrochloric acid Working Stain Solution Solution A 5 ml Solution B 25 ml Absolute Ethanol 20 ml Staining time for both of these formulations is 10 minutes, followed by rinsing for 10 min in running tap water (hematoxylin will be black) Gayle M. Callis HTL/HT/MT(ASCP) ************************ You Wrote: I do Weigert's staining for 10 minutes and use it for a week at the most. I, too, have heard conflicting times about the stability of the Weigert's, but have the best results with using it no longer than a week. Deanna Rhoads HT (ASCP) ________________________________ From: Andrea Marion <@t> uic.edu> To: histonet <@t> lists.utsouthwestern.edu Cc: itai.moshe <@t> mail.huji.ac.il Sent: Fri, September 17, 2010 10:31:16 AM Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining Hi Itai, I am interested to hear if you've resolved this problem. We use the same kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is similar to the one you mentioned. I cannot get nuclei staining with this method either. The nuclei are well stained (ie black) up to the PMA/PTA step, but during that step the nuclear stain is completely removed. I cannot shorten the PMA/PTA step without negatively effecting the collagen stain.In general, we found the original/ I have in our original protocol that the Weigert's working solution is good for one month, but I cannot recall if this is from Sigma's specification sheet or a personal observation from a lab member. However, from reading online some say it is good up to 4 months, others say it needs to be prepared fresh each time. I have tried fresh preparations with the same results. My instinct is that something is off - the staining is just not stable enough to withstand the subsequent acid steps in Masson's trichrome. Can an expert weigh in on this? Is there a way to strengthen nuclear staining from Weigert's? Sigma's formulas and usage recommendations are: Part A: 1% w/v certified Hematoxylin in ethanol Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid Combine equal volumes Part A and Part B, stain sections for 5 minutes. Andrea Marion Graduate Student University of Illinois at Chicago amario3 /at/ uic /dot/ edu Itai Moshe itai.moshe <@t> mail.huji.ac.il Wed Sep 15 11:34:51 CDT 2010 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 With sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai From histotech <@t> imagesbyhopper.com Fri Sep 17 11:41:41 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Sep 17 11:42:04 2010 Subject: [Histonet] RE: Cutting, Processing, etc In-Reply-To: <916570.85095.qm@web112601.mail.gq1.yahoo.com> Message-ID: My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention:? This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.445 / Virus Database: 271.1.1/3130 - Release Date: 09/15/10 06:34:00 From flnails <@t> texaschildrens.org Fri Sep 17 12:03:04 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Sep 17 12:03:31 2010 Subject: [Histonet] RE: Cutting, Processing, etc In-Reply-To: References: <916570.85095.qm@web112601.mail.gq1.yahoo.com> Message-ID: As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention:? This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.445 / Virus Database: 271.1.1/3130 - Release Date: 09/15/10 06:34:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From JWeems <@t> sjha.org Fri Sep 17 12:07:16 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Sep 17 12:07:28 2010 Subject: [Histonet] RE: Cutting, Processing, etc In-Reply-To: References: <916570.85095.qm@web112601.mail.gq1.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403984C06EB@CHEXCMS10.one.ads.che.org> My 2 cents is that she needed to convince someone this was how it is done! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 13:03 To: 'histotech@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention:? This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.445 / Virus Database: 271.1.1/3130 - Release Date: 09/15/10 06:34:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rosenfeldtek <@t> hotmail.com Fri Sep 17 12:15:32 2010 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Sep 17 12:15:38 2010 Subject: [Histonet] Rat heart valve histology trimming and sectioning reg In-Reply-To: <20100917083940.55478.qmail@f6mail-145-154.rediffmail.com> References: <20100917083940.55478.qmail@f6mail-145-154.rediffmail.com> Message-ID: Assuming you are talking about the aortic valve.... Remove the heart and dissect away as much surrounding fat as you can. Leave some aorta attached. Make sure the heart is very well fixed in formalin. If the heart is soft and floppy rather than firm the next step wont go well. The next step is to remove most of the ventricles--you don't want to section through all that and it just gets in the way of proper orientation during embedding. There are two ways to trim off the ventricle. The critical thing is that your sectioning plane is perpendicular to the angle at which the aorta exits the heart. The classic way is to lay the heart in the anatomical position and use a straight blade, like a razor, to cut through it, guillotine-fashion along a line drawn between the lower margins of the atria and which is perpendicular to the angle at which the aorta exits the heart. I found that method to be not so reproducible so I came up with another way. Under a dissecting scope, I use my right hand and fine forceps to suspend the heart by the stub of the aorta. Gravity then automatically pulls the heart into almost the right position for the next step. Next I use my left hand and a straight back forceps to grasp the heart just below the atria. Then trim the aorta very close to the heart. Use your fine forceps to twiddle the angle that the heart sits in the straight forceps until you are looking directly down into the aortic sinus. You should be able to see the valves. Now take a number eleven scalpel and in one or two smooth strokes, run it along the edge of the straight forceps, separating the ventricles from the top of the heart. If you do it right you will have a sort of loaf shaped piece of tissue. If you lay it flat under the scope, the stub of the aorta will be pointing straight up and you will be able to see the valves. Embed the tissue so that the caudal side is at the bottom of the embedding mold. Throw away sections until you start to see the valves. Until you have cut a few and know what you are doing it is best to start saving sections early rather than later. I like to put three sections per slide and initially stain slides 1,3,4,7,9...etc, saving the rest for possible IHC. My prefered stain for lesions is the Modified Movat Pentachrome. It's kind of a pain to do but for lesion composition analysis it can't be beat. I start lesion analysis at the point where I can see all the leaflets. Stop when only the valve stems are visible. Anyway, it will take you a while before you get reproducible, nice cross sections. That first trimming away of the ventricles makes it or breaks it. Good luck and feel free to ask clarifying questions. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Fri, 17 Sep 2010 08:39:40 +0000 > To: histonet@lists.utsouthwestern.edu > From: abijag76@rediffmail.com > Subject: [Histonet] Rat heart valve histology trimming and sectioning reg > > Dear Histonetters, > > Our pathologists are interested in rat heart valvular lesions. I am working on trimming and sectioning methods to get uniform and reproducible morphology of heart valves in paraffin sections. It seems that available literature dealing with this is limited. Requesting your valuable experience in this regard. > > > > Thanks a lot > > > > Abi jagannath > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jleroux <@t> petroglyphpath.com Fri Sep 17 12:54:42 2010 From: jleroux <@t> petroglyphpath.com (james leroux) Date: Fri Sep 17 12:58:25 2010 Subject: [Histonet] RE: Cutting, Processing, etc In-Reply-To: Message-ID: <201009171758.o8HHw7CR085170@ame7.swcp.com> Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some "supervisors" around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 11:03 AM To: 'histotech@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention:? This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.445 / Virus Database: 271.1.1/3130 - Release Date: 09/15/10 06:34:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Fri Sep 17 13:19:31 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Sep 17 13:19:36 2010 Subject: [Histonet] RE: Cutting, Processing, etc Message-ID: <20100917111931.9e2d9aa830e8449a2412eb1e4f2f067e.726fc93a4f.wbe@email04.secureserver.net> They do say that in the next 10 years 70% or so of us will be ret ired. I am 30, and a rarity it seems. I think the main issue wi people not picking up histology is because it is a fairly unknown field. have a b graduate working at Walmart doe staying in histology. I k you make, but it still isn't enou world since '98 and the pay scales real much. I think the main solution would be educated, skilled HTs out there...pay them like a skill graduate!! 35K a year for some places to start? How $400 a month in student loans and live with that salary? J cents... Oh and Amy...there are no stupid histology it's a great field. talking to yourself...it's a histo. th Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg Austin, Texas 78744 < (512)386-5 -------- Original Message -------- Subject: RE: [Histonet] RE: Cutting, Processing, etc From: "Nails, Felton" <[1]fln Date: Fri, September 17, 2010 10:03 am To: "'[2]histotech@imagesbyhop @imagesbyhopper.com>, "'mohamed abd el razik'" <[4]k84as@yahoo "[5]Histonet@lists.utsout Histonet@lists.utsouthwestern.edu> As I look through and monitor questions, it is apparent that our field is d stains or IHC st taught in histology 101. My the field, who and what will be left who ask, don't take offense to my thought up a book and read. You will improve yourself an Just my thoughts, if I offended you it was not my intent. -----Original Message----- From: [7]histonet [[8]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of [9]histotech@ Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; [10]Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It see asked these qu post, I am not so sur potential shock and dismay to the i producing the quality work that most of us empl Amy, in answer to your questions, I will echo some of the sentiments that I 1. Facing of blocks. We use one blade to face blocks and another, new blad as I can, k facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, to chill th tissue. If I get a blo calcified tissues for example), I on my waterbath and allow the hot water for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As adding more "war blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time and it does 4. Tissue processor changes. This is definitely something that is "site s small volume machine weekly, but ever machine is changed weekly. Your mileage may vary! :o) Michelle -----Original Message----- From: [11]histonet [[12]mailto:histon mohamed abd el razik< To: [13]Histonet@lists.uts Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any and we should learned alot from the of antibodies and its use t all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton <[14]flnails@texaschildrens.org> wrote: From: Nails, Felton <[15]flnai Subject: [Histonet] RE: Cutting, Processing, etc To: "[16]Histonet@lists.ut <[17]Histonet@lists.utsouthwestern.edu> Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?????????????? -----Original Message----- From: [18]histonet [[19]mailto:histon Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: [20]Histonet@lists.uts Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like here... After embedding, you face (trim) your blocks, right? Do you take sections r How many of you 'soak' your blocks in water/softblock before cutting them?< Does this depend on same? How often is "freezy" spray used in your lab, and where and when do you use How often do you rotate/change your reagents in your processors? Do you cal Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention: This Message is intended only for the use of the individua information that is p disclosure under applicable law. If the intended recipient, you are hereby n dissemination or copying of this message or the taking of in reliance on the contents of this message is strictly prohibit If you have received this message in error, please notify us immediatel _______________________________________________ Histonet mailing list [21]Histonet@lists.utsouth [22]http: ---------------------------------------------------------------------- ----- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. representative of t that any review, disseminati attachments, if any, or the informati prohibited. If you have received this e-mail i immediately notify the sender by return e-mail and delete t e-mail from your computer system. Thank you. ======================= 3D= ======================= 3D= ======================= 3D= == _______________________________________________ Histonet mailing list [23]Histonet@lists.utsouth [24]http: _______________________________________________ Histonet mailing list [25]Histonet@lists.utsouth [26]http: No virus found in this incoming message. Checked by AVG - [27]www.avg.com Version: 8.5.445 / Virus Database: 271.1.1/3130 - Release Date: 09/15/10 06 _______________________________________________ Histonet mailing list [28]Histonet@lists.utsouth [29]http: ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. 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Thank you. ======================= 3D= ======================= 3D= _______________________________________________ Histonet mailing list [30]Histonet@lists.utsouth [31]http: References 1. 3D"mailto:flnails@texaschildrens.org" 2. 3D"mailto:histotech@imagesbyhopper.com" 3. 3D"mailto:histotech@imagesbyhopper.com" 4. 3D"mailto:k84as@yahoo.com" 5. 3D"mailto:Histonet@lists.utsouthwestern.edu" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 8. 3D"mailto:histonet-bounces@l 9. 3D"mailto:histotech@imagesbyhopper.com" 10. 3D"mailto:Histonet@lists.utsouthwestern 11. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 12. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" 14. 3D"mailto:flnails@texaschild 15. 3D"mailto:flnails@texaschildrens.org" 16. 3D"mailto:Histonet@lists.utsouthwestern.edu" 17. 3D"mailto:Histonet@lists.utsouthwestern.e 18. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 19. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 20. 3D"mailto:Histonet@lists.utsouthwestern.edu" 21. 3D"mailto:Histonet@lists.utsouthwestern.edu" 22. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 23. 3D"mailto:Histonet@lists.utsouthwestern.edu" 24. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 25. 3D"mailto:Histonet@lists.utsouthwestern.edu" 26. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 27. 3D"http://www.avg.com"/ 28. 3D"mailto:Histonet@lists.utsouthwestern.edu" 29. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 30. 3D"mailto:Histonet@lists.utsouthwestern.edu" 31. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From billodonnell <@t> catholichealth.net Fri Sep 17 13:22:09 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Sep 17 13:22:25 2010 Subject: [Histonet] RE: Cutting, Processing, etc In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16403984C06EB@CHEXCMS10.one.ads.che.org> References: <916570.85095.qm@web112601.mail.gq1.yahoo.com> <92AD9B20A6C38C4587A9FEBE3A30E16403984C06EB@CHEXCMS10.one.ads.che.org> Message-ID: I echo Joyce's point. W/o all info, I hesitate to jump to conclusions. I was had an efficiency expert following me around for a week at the insistance of some administrators (it wasn't just me, but the entire lab)These are the types of silly questions these "experts" might ask, wondering if they can save 47 seconds out of the day. If anything is happening to our field, it might be the tampering by non-technicians in our technical duties in the name of "stream-lining" or keeping their tush's covered for their boss who is keeping her tush covered from ad nausium. It's a cynical Friday....but the good news is that my Kindle is being delivered today! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 17, 2010 12:07 PM To: Nails, Felton; 'histotech@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My 2 cents is that she needed to convince someone this was how it is done! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 13:03 To: 'histotech@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention:? This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.445 / Virus Database: 271.1.1/3130 - Release Date: 09/15/10 06:34:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From janderson <@t> halozyme.com Fri Sep 17 13:36:04 2010 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Fri Sep 17 13:36:21 2010 Subject: [Histonet] unsubscribe Message-ID: <5F7CC9B788911848A79BC83453A3D65304147317E2@Tomlinson.hti.com> Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) janderson@halozyme.com ________________________________ The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. From gayle.callis <@t> bresnan.net Fri Sep 17 13:43:45 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Sep 17 13:44:02 2010 Subject: [Histonet] Rat heart valve histology trimming and sectioning reg In-Reply-To: References: <20100917083940.55478.qmail@f6mail-145-154.rediffmail.com> Message-ID: <000d01cb5698$43db89f0$cb929dd0$@callis@bresnan.net> I just sent Abi a pdf of the publication he wanted. Joanna Barton and her colleagues developed a very nice way to use Histogel in a special way to optimize examination of rodent heart valves. After initial preparation/fixation they sliced the rat hearts using an acrylic rat heart matrix (slicing device) fronm Zivic Instruments. They ended up with three heart sections for further processing into paraffin. The photomicrographs showed excellent preservation of these very delicate but totally intact structures. I was very impressed at the quality of their work. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, September 17, 2010 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Rat heart valve histology trimming and sectioning reg Assuming you are talking about the aortic valve.... Remove the heart and dissect away as much surrounding fat as you can. Leave some aorta attached. Make sure the heart is very well fixed in formalin. If the heart is soft and floppy rather than firm the next step wont go well. The next step is to remove most of the ventricles--you don't want to section through all that and it just gets in the way of proper orientation during embedding. There are two ways to trim off the ventricle. The critical thing is that your sectioning plane is perpendicular to the angle at which the aorta exits the heart. The classic way is to lay the heart in the anatomical position and use a straight blade, like a razor, to cut through it, guillotine-fashion along a line drawn between the lower margins of the atria and which is perpendicular to the angle at which the aorta exits the heart. I found that method to be not so reproducible so I came up with another way. Under a dissecting scope, I use my right hand and fine forceps to suspend the heart by the stub of the aorta. Gravity then automatically pulls the heart into almost the right position for the next step. Next I use my left hand and a straight back forceps to grasp the heart just below the atria. Then trim the aorta very close to the heart. Use your fine forceps to twiddle the angle that the heart sits in the straight forceps until you are looking directly down into the aortic sinus. You should be able to see the valves. Now take a number eleven scalpel and in one or two smooth strokes, run it along the edge of the straight forceps, separating the ventricles from the top of the heart. If you do it right you will have a sort of loaf shaped piece of tissue. If you lay it flat under the scope, the stub of the aorta will be pointing straight up and you will be able to see the valves. Embed the tissue so that the caudal side is at the bottom of the embedding mold. Throw away sections until you start to see the valves. Until you have cut a few and know what you are doing it is best to start saving sections early rather than later. I like to put three sections per slide and initially stain slides 1,3,4,7,9...etc, saving the rest for possible IHC. My prefered stain for lesions is the Modified Movat Pentachrome. It's kind of a pain to do but for lesion composition analysis it can't be beat. I start lesion analysis at the point where I can see all the leaflets. Stop when only the valve stems are visible. Anyway, it will take you a while before you get reproducible, nice cross sections. That first trimming away of the ventricles makes it or breaks it. Good luck and feel free to ask clarifying questions. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Fri, 17 Sep 2010 08:39:40 +0000 > To: histonet@lists.utsouthwestern.edu > From: abijag76@rediffmail.com > Subject: [Histonet] Rat heart valve histology trimming and sectioning reg > > Dear Histonetters, > > Our pathologists are interested in rat heart valvular lesions. I am working on trimming and sectioning methods to get uniform and reproducible morphology of heart valves in paraffin sections. It seems that available literature dealing with this is limited. Requesting your valuable experience in this regard. > > > > Thanks a lot > > > > Abi jagannath > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5458 (20100917) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5458 (20100917) __________ The message was checked by ESET Smart Security. http://www.eset.com From eberledelphine <@t> hotmail.com Fri Sep 17 14:13:21 2010 From: eberledelphine <@t> hotmail.com (delphine eberle) Date: Fri Sep 17 14:13:28 2010 Subject: [Histonet] Autofluorescence problem on atheroma - TUNEL staining Message-ID: Hi, Autofluorescence in atheroma is a well known problem for immunofluorescence staining. I would love to hear input about how to reduce it to a minimum (if possible!). So far, we are trying to detect apoptotic cells in atheroma by TUNEL staining (Roche, TMRred) and we found the interpretation very difficult as some area of the tissue show red dots, tat could be considered as apoptotic bodies. We are working with frozen sections, fixing with Formalin, quenching with Glycine etc... -I was wondering if acetone fixation (or other fixation methods) could decrease atheroma autofluorescence by extracting lipids and others materials? -Also, I am not sure that acetone fixation is recommended for TUNEL staining? Did anybody try? -Are there around any approved ways to decrease autofluorescence in atheroma? (I can not find anything!) Thanks! Delphine Delphine Eberl? PhD UCSF Department of Vascular Surgery VA Medical Center - NCIRE Building 2 - room 410 4150 Clement Street San Francisco, CA 94121, USA Tel: 415 221 4810 ext.2984 Cell: 857 453 0821 delphine.eberle@ucsfmedctr.org From Maria.Katleba <@t> stjoe.org Fri Sep 17 14:20:58 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Sep 17 14:23:21 2010 Subject: [Histonet] UKAIH- Temp or Traveling Histotech needed asap!!! In-Reply-To: References: Message-ID: Calling all histotechs who need a few extra hours!!!!!!! A very good friend of mine is in need of a temp or traveling histotech to cover the histology duties for 2 weeks... potentially one month. STARTING NOW! Low volume (40-60 blocks) For more details call Shawn 707-463-7301 Clinical Lab Supervisor or the histology lab asst Pam at 707-462-3111 x1480 The pathologist is very sweet, I love him! I have done work for him before. I live 2 hours away, so there's no way I can commute and manage my lab as well. Hours are 8am to 430pm... (or less if you can get it done faster) Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From thomas.crowell <@t> novartis.com Fri Sep 17 14:58:34 2010 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Fri Sep 17 14:58:41 2010 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 09/17/2010 and will not return until 09/28/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From higginst <@t> amapath.com Fri Sep 17 15:08:08 2010 From: higginst <@t> amapath.com (Tim Higgins) Date: Fri Sep 17 15:12:18 2010 Subject: [Histonet] Ventana Ultraview Detection Message-ID: <000001cb56a4$0d539d80$6a03a8c0@apg> Hello Histonetters, We are in the process of selling some of our Ventana Ultraview Detection kits. The kits still have a long shelf life and have not been registered. If you are interested in purchasing one of more of these kits please email me with questions and or contact info. Thanks, Timothy Higgins, HT (ASCP) QIHC Histology Manager APA Amarillo, TX From tjasper <@t> copc.net Fri Sep 17 16:08:56 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Sep 17 16:09:06 2010 Subject: [Histonet] RE: Cutting, Processing, etc References: <201009171758.o8HHw7CR085170@ame7.swcp.com> Message-ID: <90354A475B420441B2A0396E5008D49692BF99@copc-sbs.COPC.local> Hi James, I would take it a step farther with the continuing ed. I think it's beyond the supervisors it gets up into lab administration (clinical lab world). I personally know of a group of great folks that work hard and run a quality service. In the last 3 years they've had a major drop off in their continuing ed. And it, of course, is tied to the budget. Unfortunately, in this case (my view) those making the money decisions are missing the value. It seems they're unwilling to make the investment. I fear that in 5 years or less (if it continues) this service will suffer. I suspect there are other folks out there in the same boat. My hat is off to everyone out there working hard in our field and to the "enlightened" administrators and physicians that advocate continuing ed. Have a great weekend. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of james leroux Sent: Friday, September 17, 2010 10:55 AM To: 'Nails, Felton'; histotech@imagesbyhopper.com; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some "supervisors" around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 11:03 AM To: 'histotech@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention:? This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? 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Checked by AVG - www.avg.com Version: 8.5.445 / Virus Database: 271.1.1/3130 - Release Date: 09/15/10 06:34:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ctorrence <@t> kmcpa.com Fri Sep 17 16:14:07 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Fri Sep 17 16:14:21 2010 Subject: [Histonet] Out of Office Reply Message-ID: <55e0e5d1172240289a40da6d15e52b60@2b2d7372afb34816ae046d96501fa306> I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From macveigh <@t> usc.edu Fri Sep 17 16:20:31 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Fri Sep 17 16:20:29 2010 Subject: [Histonet] Is anybody using TBS ATP1 - 120 tissue processor? Message-ID: <46A700EDEA6641FD8BD364CDEEA54C9E@DFS66DD1> Hi all, Would you please let me know off line what do you think about this unit? macveigh@usc.edu Thank you very much in advance Michelle Aloni MS HTL ASCP From MW <@t> PersonifySearch.com Fri Sep 17 16:40:06 2010 From: MW <@t> PersonifySearch.com (Matthew Ward) Date: Fri Sep 17 16:40:16 2010 Subject: [Histonet] Histology Training Specialist in the Chicago Area!! Message-ID: <01a601cb56b0$e5bbbcf0$b13336d0$@com> Good Afternoon! Please contact me directly for more information on this position or to learn more about our other opportunities. Have a great weekend! Histology Training Specialist The Company: Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. The Opportunity: The company currently has an opening for a Histology Training Specialist. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: $55-60k Other: Full benefits - 401k program/matching Primary Responsibilities: The primary responsibility of this role will be to provide technical phone support by answering questions, troubleshooting problems, logging and closing complaint files and escalating major issues to appropriate company personnel. This role will also provide technical training on specified products in the company's newly constructed state-of-the-art Customer Support Laboratory. Training programs are designed for small groups to ensure maximum customer learning and satisfaction. Additional Responsibilities: - Provide product and applications phone support to end-users, field personnel and dealers for all product lines - Log all calls into Customer Support Database - Participate in development of training materials and conduct classes and labs for customers, employees and others as needed - Serve as technical liaison to Customer Service/Field Service/Product Management departments Education and Experience Required: Ability to interact with various people in a calm and positive fashion and the ability to effectively communicate information to groups of participants is required. Experience with data entry, MS Office programs (PowerPoint, Lotus Notes, Word) is also required. HT/HTL/QIHC (ASCP) is helpful but not required. Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From swongman <@t> yahoo.com Sat Sep 18 00:48:10 2010 From: swongman <@t> yahoo.com (Steve Wong) Date: Sat Sep 18 00:48:20 2010 Subject: [Histonet] In search of CRO specializing in volumetric determination via image analysis on serial sections. Message-ID: <531599.64936.qm@web56205.mail.re3.yahoo.com> Looking for a CRO experienced in determining the volume of an irregularly shaped object from serial sections. Automation experience a plus. From itai.moshe <@t> mail.huji.ac.il Sat Sep 18 09:03:23 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Sat Sep 18 09:04:44 2010 Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining In-Reply-To: <528158.30084.qm@web84308.mail.re1.yahoo.com> References: <3363065ee4098fba6e4ee9ad0804e9f8.squirrel@webmail.uic.edu> <528158.30084.qm@web84308.mail.re1.yahoo.com> Message-ID: Hi, and thank's for the quick replay. I'm using a fresh Weigert's prepared from the sigma kit every time. maybe it is possible to modify and to use sirius red to stain for collage. or to use methyl green for the nuclei. Maye the bluing part is not done o.k, maybe like Mr' Madary offered, a warm tap water will do the job ? is it that important to use running tap water, and not just to immerse the slides in tap water, because i don't have a proper bath to do this ? Itai 2010/9/17 Deanna Rhoads > I do Weigert's staining for 10 minutes and use it for a week at the most. > I, too, have heard conflicting times about the stability of the Weigert's, > but have the best results with using it no longer than a week. > > Deanna Rhoads HT (ASCP) > > ------------------------------ > *From:* Andrea Marion > > *To:* histonet@lists.utsouthwestern.edu > *Cc:* itai.moshe@mail.huji.ac.il > *Sent:* Fri, September 17, 2010 10:31:16 AM > *Subject:* [Histonet] Re: Masson's trichrom - problem with nuclei staining > > Hi Itai, > > I am interested to hear if you've resolved this problem. We use the same > kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is > similar to the one you mentioned. I cannot get nuclei staining with this > method either. The nuclei are well stained (ie black) up to the PMA/PTA > step, but during that step the nuclear stain is completely removed. I > cannot shorten the PMA/PTA step without negatively effecting the collagen > stain. > > I have in our original protocol that the Weigert's working solution is > good for one month, but I cannot recall if this is from Sigma's > specification sheet or a personal observation from a lab member. However, > from reading online some say it is good up to 4 months, others say it > needs to be prepared fresh each time. I have tried fresh preparations with > the same results. > > My instinct is that something is off - the staining is just not stable > enough to withstand the subsequent acid steps in Masson's trichrome. Can > an expert weigh in on this? Is there a way to strengthen nuclear staining > from Weigert's? > > Sigma's formulas and usage recommendations are: > > Part A: 1% w/v certified Hematoxylin in ethanol > Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid > > Combine equal volumes Part A and Part B, stain sections for 5 minutes. > > > Andrea Marion > Graduate Student > University of Illinois at Chicago > amario3 /at/ uic /dot/ edu > > 010/9/15 Madary, Joseph > I never use the Masson and expect decent nuclear staining. Do not forget > to mordant in Bouins, wash it out well, and the running water or sitting in > several changes of warm tap water is a bluing step. > > > > Nick Madary, HT/HTL(ASCP)QIHC > > Histology Mgr, Medimmune > > 301.398.6360(lab), 4745(vm),9745(fax) > 010/9/15 Patricia F Lott > Make sure when you mix solution a and solution b, you have a *very dark > purple color* solution that smells fruity, like wine. If not, check that > your solutions are not expired. I buy the Weigert?s kit (32 oz. of Sol. A > and 32 oz. of Sol.B) from *Poly Scientific*, and it works very well. > > > > The purpose of the tap water is for blueing, it will make the nuclei very > dark and crisp if you leave the whole slide rack in a sink with running tap > water for 10 minutes. > > > > Best Regards, > > Patty Lott > > 2010/9/16 Jack Ratliff > >> The tap water rinse is in reference to running tap water (not directly on >> the slide sections) while the slides sit in a make-shift water bath. This is >> done over a period of time to differentiate the nuclei staining similar to >> a "bluing" step when working with an alum hematoxylin in a standard H&E. >> Also keep in mind that improper fixation can also contribute to poor nuclei >> staining. >> >> Jack >> > > Itai Moshe itai.moshe <@t> mail.huji.ac.il > Wed Sep 15 11:34:51 CDT 2010 > > Hi Histonet's > > I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with > PFA. > I'm using this protocol: > http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 > With sigma masson's > kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) > The staining is beautiful, but i can't see the nuclei good enough. > 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only > reason that i"m not using the simpler sirius red and fast green staining.) > 2) What is the meaning for washing in running tap water washing, is it done > by putting the slides in a jar with simple tap water for a few minutes ? > > Thank's > Itai > > P.S > By mistake I've post this before in another message with a wrong title. > please respond to that message, so the title will be o.k for future archive > searching. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From itai.moshe <@t> mail.huji.ac.il Sat Sep 18 09:03:23 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Sat Sep 18 09:10:34 2010 Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining In-Reply-To: <528158.30084.qm@web84308.mail.re1.yahoo.com> References: <3363065ee4098fba6e4ee9ad0804e9f8.squirrel@webmail.uic.edu> <528158.30084.qm@web84308.mail.re1.yahoo.com> Message-ID: Hi, and thank's for the quick replay. I'm using a fresh Weigert's prepared from the sigma kit every time. maybe it is possible to modify and to use sirius red to stain for collage. or to use methyl green for the nuclei. Maye the bluing part is not done o.k, maybe like Mr' Madary offered, a warm tap water will do the job ? is it that important to use running tap water, and not just to immerse the slides in tap water, because i don't have a proper bath to do this ? Itai 2010/9/17 Deanna Rhoads > I do Weigert's staining for 10 minutes and use it for a week at the most. > I, too, have heard conflicting times about the stability of the Weigert's, > but have the best results with using it no longer than a week. > > Deanna Rhoads HT (ASCP) > > ------------------------------ > *From:* Andrea Marion > > *To:* histonet@lists.utsouthwestern.edu > *Cc:* itai.moshe@mail.huji.ac.il > *Sent:* Fri, September 17, 2010 10:31:16 AM > *Subject:* [Histonet] Re: Masson's trichrom - problem with nuclei staining > > Hi Itai, > > I am interested to hear if you've resolved this problem. We use the same > kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is > similar to the one you mentioned. I cannot get nuclei staining with this > method either. The nuclei are well stained (ie black) up to the PMA/PTA > step, but during that step the nuclear stain is completely removed. I > cannot shorten the PMA/PTA step without negatively effecting the collagen > stain. > > I have in our original protocol that the Weigert's working solution is > good for one month, but I cannot recall if this is from Sigma's > specification sheet or a personal observation from a lab member. However, > from reading online some say it is good up to 4 months, others say it > needs to be prepared fresh each time. I have tried fresh preparations with > the same results. > > My instinct is that something is off - the staining is just not stable > enough to withstand the subsequent acid steps in Masson's trichrome. Can > an expert weigh in on this? Is there a way to strengthen nuclear staining > from Weigert's? > > Sigma's formulas and usage recommendations are: > > Part A: 1% w/v certified Hematoxylin in ethanol > Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid > > Combine equal volumes Part A and Part B, stain sections for 5 minutes. > > > Andrea Marion > Graduate Student > University of Illinois at Chicago > amario3 /at/ uic /dot/ edu > > 010/9/15 Madary, Joseph > I never use the Masson and expect decent nuclear staining. Do not forget > to mordant in Bouins, wash it out well, and the running water or sitting in > several changes of warm tap water is a bluing step. > > > > Nick Madary, HT/HTL(ASCP)QIHC > > Histology Mgr, Medimmune > > 301.398.6360(lab), 4745(vm),9745(fax) > 010/9/15 Patricia F Lott > Make sure when you mix solution a and solution b, you have a *very dark > purple color* solution that smells fruity, like wine. If not, check that > your solutions are not expired. I buy the Weigert?s kit (32 oz. of Sol. A > and 32 oz. of Sol.B) from *Poly Scientific*, and it works very well. > > > > The purpose of the tap water is for blueing, it will make the nuclei very > dark and crisp if you leave the whole slide rack in a sink with running tap > water for 10 minutes. > > > > Best Regards, > > Patty Lott > > 2010/9/16 Jack Ratliff > >> The tap water rinse is in reference to running tap water (not directly on >> the slide sections) while the slides sit in a make-shift water bath. This is >> done over a period of time to differentiate the nuclei staining similar to >> a "bluing" step when working with an alum hematoxylin in a standard H&E. >> Also keep in mind that improper fixation can also contribute to poor nuclei >> staining. >> >> Jack >> > > Itai Moshe itai.moshe <@t> mail.huji.ac.il > Wed Sep 15 11:34:51 CDT 2010 > > Hi Histonet's > > I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with > PFA. > I'm using this protocol: > http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 > With sigma masson's > kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) > The staining is beautiful, but i can't see the nuclei good enough. > 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only > reason that i"m not using the simpler sirius red and fast green staining.) > 2) What is the meaning for washing in running tap water washing, is it done > by putting the slides in a jar with simple tap water for a few minutes ? > > Thank's > Itai > > P.S > By mistake I've post this before in another message with a wrong title. > please respond to that message, so the title will be o.k for future archive > searching. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amario3 <@t> uic.edu Sat Sep 18 11:34:42 2010 From: amario3 <@t> uic.edu (Andrea Marion) Date: Sat Sep 18 11:34:50 2010 Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining In-Reply-To: References: <3363065ee4098fba6e4ee9ad0804e9f8.squirrel@webmail.uic.edu> <528158.30084.qm@web84308.mail.re1.yahoo.com> Message-ID: Hi Itai, I am thinking that we will purchase a different Weigert's from another company, or try one of the modified ones that were recommended on the listserv. Running tap water only means that you are replacing the water the slides are sitting in continuously, so that they have a constant supply of fresh water. This removes any excess stain that may be bleeding from the slide, and prevents it from recoloring the water, and restaining the tissue. Essentially it helps pull more excess stain out of the slide. Leaving it in tap water does not have the same effect, because the dye will reach an equilibrium point whereby no more will be removed from the tissue. We use a coplin jar that we stain the slides in, and simply move it to the sink and let water run into the jar gently. You can achieve the same thing if you do not have a sink of some sort by constantly dumping out the water the slides are in, and replacing with clean water. It doesn't require any special equipment, just a source of running water and whatever container you normally stain slides in. Andrea On Sat, September 18, 2010 9:03 am, Itai Moshe wrote: > Hi, and thank's for the quick replay. > > I'm using a fresh Weigert's prepared from the sigma kit every time. > maybe it is possible to modify and to use sirius red to stain for > collage. or to use methyl green for the nuclei. > > Maye the bluing part is not done o.k, maybe like Mr' Madary offered, a > warm > tap water will do the job ? > is it that important to use running tap water, and not just to immerse the > slides in tap water, because i don't have a proper bath to do this ? > > Itai > > 2010/9/17 Deanna Rhoads > >> I do Weigert's staining for 10 minutes and use it for a week at the >> most. >> I, too, have heard conflicting times about the stability of the >> Weigert's, >> but have the best results with using it no longer than a week. >> >> Deanna Rhoads HT (ASCP) >> >> ------------------------------ >> *From:* Andrea Marion >> >> *To:* histonet@lists.utsouthwestern.edu >> *Cc:* itai.moshe@mail.huji.ac.il >> *Sent:* Fri, September 17, 2010 10:31:16 AM >> *Subject:* [Histonet] Re: Masson's trichrom - problem with nuclei >> staining >> >> Hi Itai, >> >> I am interested to hear if you've resolved this problem. We use the >> same >> kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is >> similar to the one you mentioned. I cannot get nuclei staining with this >> method either. The nuclei are well stained (ie black) up to the PMA/PTA >> step, but during that step the nuclear stain is completely removed. I >> cannot shorten the PMA/PTA step without negatively effecting the >> collagen >> stain. >> >> I have in our original protocol that the Weigert's working solution is >> good for one month, but I cannot recall if this is from Sigma's >> specification sheet or a personal observation from a lab member. >> However, >> from reading online some say it is good up to 4 months, others say it >> needs to be prepared fresh each time. I have tried fresh preparations >> with >> the same results. >> >> My instinct is that something is off - the staining is just not stable >> enough to withstand the subsequent acid steps in Masson's trichrome. Can >> an expert weigh in on this? Is there a way to strengthen nuclear >> staining >> from Weigert's? >> >> Sigma's formulas and usage recommendations are: >> >> Part A: 1% w/v certified Hematoxylin in ethanol >> Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid >> >> Combine equal volumes Part A and Part B, stain sections for 5 minutes. >> >> >> Andrea Marion >> Graduate Student >> University of Illinois at Chicago >> amario3 /at/ uic /dot/ edu >> >> 010/9/15 Madary, Joseph > >> I never use the Masson and expect decent nuclear staining. Do not >> forget >> to mordant in Bouins, wash it out well, and the running water or sitting >> in >> several changes of warm tap water is a bluing step. >> >> >> >> Nick Madary, HT/HTL(ASCP)QIHC >> >> Histology Mgr, Medimmune >> >> 301.398.6360(lab), 4745(vm),9745(fax) >> > > 010/9/15 Patricia F Lott > >> Make sure when you mix solution a and solution b, you have a *very dark >> purple color* solution that smells fruity, like wine. If not, check >> that >> your solutions are not expired. I buy the Weigert?s kit (32 oz. of Sol. >> A >> and 32 oz. of Sol.B) from *Poly Scientific*, and it works very well. >> >> >> >> The purpose of the tap water is for blueing, it will make the nuclei >> very >> dark and crisp if you leave the whole slide rack in a sink with running >> tap >> water for 10 minutes. >> >> >> >> Best Regards, >> >> Patty Lott >> > > >> 2010/9/16 Jack Ratliff >> >>> The tap water rinse is in reference to running tap water (not directly >>> on >>> the slide sections) while the slides sit in a make-shift water bath. >>> This is >>> done over a period of time to differentiate the nuclei staining similar >>> to >>> a "bluing" step when working with an alum hematoxylin in a standard >>> H&E. >>> Also keep in mind that improper fixation can also contribute to poor >>> nuclei >>> staining. >>> >>> Jack >>> >> > >> Itai Moshe itai.moshe <@t> mail.huji.ac.il >> Wed Sep 15 11:34:51 CDT 2010 >> >> Hi Histonet's >> >> I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with >> PFA. >> I'm using this protocol: >> http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 >> With sigma >> masson's >> kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) >> The staining is beautiful, but i can't see the nuclei good enough. >> 1) Is there a way to enhance the nuclei staining ? (the nuclei is the >> only >> reason that i"m not using the simpler sirius red and fast green >> staining.) >> 2) What is the meaning for washing in running tap water washing, is it >> done >> by putting the slides in a jar with simple tap water for a few minutes >> ? >> >> Thank's >> Itai >> >> P.S >> By mistake I've post this before in another message with a wrong title. >> please respond to that message, so the title will be o.k for future >> archive >> searching. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From ctorrence <@t> kmcpa.com Sat Sep 18 12:04:25 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Sat Sep 18 12:04:38 2010 Subject: [Histonet] Out of Office Reply Message-ID: I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From cdisbrow <@t> msn.com Sat Sep 18 12:25:37 2010 From: cdisbrow <@t> msn.com (Carrie Disbrow) Date: Sat Sep 18 12:25:41 2010 Subject: [Histonet] Posting comments In-Reply-To: References: Message-ID: As a newbie trying to learn as much as possible, I'm disappointed when questions are posted and the responses are sent to the individual. I learn more when the responses are shared. Also, an archive search on the subject will have limited information. I would think there are many in the same boat - those who want to learn more about the craft. Even a review of the basics is good reading for me. That is why I "reply all" instead of "reply." From c.m.vanderloos <@t> amc.uva.nl Sat Sep 18 12:53:02 2010 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Sat Sep 18 12:53:13 2010 Subject: [Histonet] RE: Autofluorescence problem on atheroma - TUNEL Message-ID: Dear Delphine, Reading your message I was wondering in the first place why TUNEL? Cleaved caspase-3 antibody available from several vendors is doing a marvelous job and is way much easier than TUNEL. Besides that there is literature available that caspase-3 IHC demonstrates less false positive signal than TUNEL. Unfortunately, there are still old fashioned reviewers who stick to TUNEL as 'gold standard'. I guess that formalin-fixation of the cryo's is needed and I am almost sure that acetone is not working for TUNEL. Paraffin tissue sections are to my idea much better for TUNEL than cryo's. Having said that you better switch to enzymatic visualization rather than fluorescence. The formalin causes massive autofluorescence that is hard to kill. And if you get it killed you may ask yourself if your specific signal is perhaps also killed. Use tonsil (follicle center) as positive control for TUNEL or caspase-3. Hope this helps a bit. Cheers, Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Fri, 17 Sep 2010 21:13:21 +0200 From: delphine eberle Subject: [Histonet] Autofluorescence problem on atheroma - TUNEL staining To: histonet histonet@lists.utsouthwestern.edu Hi, Autofluorescence in atheroma is a well known problem for immunofluorescence staining. I would love to hear input about how to reduce it to a minimum (if possible!). So far, we are trying to detect apoptotic cells in atheroma by TUNEL staining (Roche, TMRred) and we found the interpretation very difficult as some area of the tissue show red dots, tat could be considered as apoptotic bodies. We are working with frozen sections, fixing with Formalin, quenching with Glycine etc... -I was wondering if acetone fixation (or other fixation methods) could decrease atheroma autofluorescence by extracting lipids and others materials? -Also, I am not sure that acetone fixation is recommended for TUNEL staining? Did anybody try? -Are there around any approved ways to decrease autofluorescence in atheroma? (I can not find anything!) Thanks! Delphine Delphine Eberl? PhD UCSF Department of Vascular Surgery VA Medical Center - NCIRE Building 2 - room 410 4150 Clement Street San Francisco, CA 94121, USA Tel: 415 221 4810 ext.2984 Cell: 857 453 0821 delphine.eberle@ucsfmedctr.org From Ken_Marissael <@t> vwr.com Sat Sep 18 13:01:19 2010 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Sat Sep 18 13:01:31 2010 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 09/18/2010 and will not return until 09/27/2010. In my absense, Jackie Zerillo will be covering for me. She can be reached at jaclyn_zerillo@vwr.com or by cell at 609-410-6152. From MadaryJ <@t> MedImmune.com Sat Sep 18 13:19:54 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Sat Sep 18 13:20:03 2010 Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining In-Reply-To: References: <3363065ee4098fba6e4ee9ad0804e9f8.squirrel@webmail.uic.edu><528158.30084.qm@web84308.mail.re1.yahoo.com> Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1302681E2E@MD1EV002.medimmune.com> I do tap watewr because it works without wasting water and all of the water running could bring out some chemicals that affect the subsequent staining. Truth is most peopl;e never consider nulcei as an impolrtant step of the masson. If you want to do a faster stain do the van Gieson. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) ________________________________ From: Itai Moshe [mailto:itai.moshe@mail.huji.ac.il] Sent: Saturday, September 18, 2010 10:03 AM To: deannal78@verizon.net; amario3@uic.edu; histonet@lists.utsouthwestern.edu; Madary, Joseph; plott@uab.edu; atliffjack@hotmail.com Subject: Re: [Histonet] Re: Masson's trichrom - problem with nuclei staining Hi, and thank's for the quick replay. I'm using a fresh Weigert's prepared from the sigma kit every time. maybe it is possible to modify and to use sirius red to stain for collage. or to use methyl green for the nuclei. Maye the bluing part is not done o.k, maybe like Mr' Madary offered, a warm tap water will do the job ? is it that important to use running tap water, and not just to immerse the slides in tap water, because i don't have a proper bath to do this ? Itai 2010/9/17 Deanna Rhoads I do Weigert's staining for 10 minutes and use it for a week at the most. I, too, have heard conflicting times about the stability of the Weigert's, but have the best results with using it no longer than a week. Deanna Rhoads HT (ASCP) ________________________________ From: Andrea Marion To: histonet@lists.utsouthwestern.edu Cc: itai.moshe@mail.huji.ac.il Sent: Fri, September 17, 2010 10:31:16 AM Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining Hi Itai, I am interested to hear if you've resolved this problem. We use the same kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is similar to the one you mentioned. I cannot get nuclei staining with this method either. The nuclei are well stained (ie black) up to the PMA/PTA step, but during that step the nuclear stain is completely removed. I cannot shorten the PMA/PTA step without negatively effecting the collagen stain. I have in our original protocol that the Weigert's working solution is good for one month, but I cannot recall if this is from Sigma's specification sheet or a personal observation from a lab member. However, from reading online some say it is good up to 4 months, others say it needs to be prepared fresh each time. I have tried fresh preparations with the same results. My instinct is that something is off - the staining is just not stable enough to withstand the subsequent acid steps in Masson's trichrome. Can an expert weigh in on this? Is there a way to strengthen nuclear staining from Weigert's? Sigma's formulas and usage recommendations are: Part A: 1% w/v certified Hematoxylin in ethanol Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid Combine equal volumes Part A and Part B, stain sections for 5 minutes. Andrea Marion Graduate Student University of Illinois at Chicago amario3 /at/ uic /dot/ edu 010/9/15 Madary, Joseph I never use the Masson and expect decent nuclear staining. Do not forget to mordant in Bouins, wash it out well, and the running water or sitting in several changes of warm tap water is a bluing step. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) 010/9/15 Patricia F Lott Make sure when you mix solution a and solution b, you have a very dark purple color solution that smells fruity, like wine. If not, check that your solutions are not expired. I buy the Weigert's kit (32 oz. of Sol. A and 32 oz. of Sol.B) from Poly Scientific, and it works very well. The purpose of the tap water is for blueing, it will make the nuclei very dark and crisp if you leave the whole slide rack in a sink with running tap water for 10 minutes. Best Regards, Patty Lott 2010/9/16 Jack Ratliff The tap water rinse is in reference to running tap water (not directly on the slide sections) while the slides sit in a make-shift water bath. This is done over a period of time to differentiate the nuclei staining similar to a "bluing" step when working with an alum hematoxylin in a standard H&E. Also keep in mind that improper fixation can also contribute to poor nuclei staining. Jack Itai Moshe itai.moshe <@t> mail.huji.ac.il Wed Sep 15 11:34:51 CDT 2010 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 With sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai P.S By mistake I've post this before in another message with a wrong title. please respond to that message, so the title will be o.k for future archive searching. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From m.sokol37 <@t> gmail.com Sat Sep 18 22:45:52 2010 From: m.sokol37 <@t> gmail.com (Melinda Sokol) Date: Sat Sep 18 22:45:56 2010 Subject: [Histonet] CBG Recycler Message-ID: Hi All I would like Pros and Cons regarding a CBG Recycler. Please- No Vendors. Thanks Melinda A Hamilton HT (ASCP) From ctorrence <@t> kmcpa.com Sun Sep 19 12:05:48 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Sun Sep 19 12:05:59 2010 Subject: [Histonet] Out of Office Reply Message-ID: I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From max_histo_00 <@t> yahoo.it Sun Sep 19 17:41:13 2010 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sun Sep 19 17:41:19 2010 Subject: [Histonet] How long ... Message-ID: <434119.85179.qm@web29603.mail.ird.yahoo.com> Hi all, I found into a shelf of the laboratory a small flask containing mouse samples fixed in Bouin's fluid and preserved in ethanol at 70?, forgotten there for a few years. I wonder if it would be possible to continue the process up to paraffin embedding for histological preparations. Some time ago a professor of biology at the University of Florence told me that they could remain in alcohol for years, but on literature I found that the time can not be so long without altering tissues. Has anyone had a similar experience? It's better to throw everything away or not? Thank you in advance. Kind Regards, Massimo Tosi From max_histo_00 <@t> yahoo.it Sun Sep 19 17:41:13 2010 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sun Sep 19 17:41:21 2010 Subject: [Histonet] How long ... Message-ID: <434119.85179.qm@web29603.mail.ird.yahoo.com> Hi all, I found into a shelf of the laboratory a small flask containing mouse samples fixed in Bouin's fluid and preserved in ethanol at 70?, forgotten there for a few years. I wonder if it would be possible to continue the process up to paraffin embedding for histological preparations. Some time ago a professor of biology at the University of Florence told me that they could remain in alcohol for years, but on literature I found that the time can not be so long without altering tissues. Has anyone had a similar experience? It's better to throw everything away or not? Thank you in advance. Kind Regards, Massimo Tosi From max_histo_00 <@t> yahoo.it Sun Sep 19 17:48:44 2010 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sun Sep 19 17:48:48 2010 Subject: [Histonet] How long ... Message-ID: <783206.6696.qm@web29601.mail.ird.yahoo.com> I found into a shelf of the laboratory a small flask containing mouse samples fixed in Bouin's fluid and preserved in ethanol at 70?, forgotten there for a few years. I wonder if it would be possible to continue the process up to paraffin embedding for histological preparations. Some time ago a professor of biology at the University of Florence told me that they could remain in alcohol for years, but on literature I found that the time can not be so long without altering tissues. Has anyone had a similar experience? It's better to throw everything away or not? Thank you in advance. Kind Regards, Massimo From AnthonyH <@t> chw.edu.au Sun Sep 19 18:17:44 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Sep 19 18:18:08 2010 Subject: [Histonet] ANP. 23075 In-Reply-To: Message-ID: Hi all, Weekend fridge temps are a problem. For critical fridges (Mortuary, -70oC, antibody etc), I would attache the temp logger to an alarm so if the temp goes out of range, you will be warned out of hours and have time to save the day. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, 17 September 2010 12:32 AM To: 'Laurie Colbert'; Kaye Ryan; Rathborne,Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 By using a thermometer that records high and low temperatures, you can see if your fridge or freezer went out-of-range over the weekend. This satisfies CAP Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:27 AM To: Kaye Ryan; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I guess there's nothing you can do. -----Original Message----- From: Kaye Ryan [mailto:kryan@nfderm.com] Sent: Thursday, September 16, 2010 7:23 AM To: Laurie Colbert; Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 How do you handle this if you have no one that comes in on the weekend? Kaye -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 16, 2010 10:10 AM To: Rathborne, Toni; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 Someone from the Clinical Lab records temps. -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, September 16, 2010 5:14 AM To: Laurie Colbert; Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 If you do not have staff in on weekends/holidays, how do you monitor your temperatures then? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, September 15, 2010 4:51 PM To: Kathy M. Gorham; ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. 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Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. 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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From itai.moshe <@t> mail.huji.ac.il Mon Sep 20 03:45:58 2010 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Mon Sep 20 03:46:08 2010 Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining In-Reply-To: <-3224673474534940328@unknownmsgid> References: <3363065ee4098fba6e4ee9ad0804e9f8.squirrel@webmail.uic.edu> <528158.30084.qm@web84308.mail.re1.yahoo.com> <-3224673474534940328@unknownmsgid> Message-ID: Hi, What concentration of HCL are you using in solution B ? is it 1% in the end, so i will have to use 3ml of the standard 36% HCL in 100ml, not just 1ml as mentioned in the protocols ? Itai *gayle callis** **gayle.callis <@t> bresnan.net * * **Fri Sep 17 11:40:13 CDT 2010* - *Previous message: **[Histonet] Re: Masson's trichrom - problem with nuclei staining* - *Next message: **[Histonet] RE: Cutting, Processing, etc* - *Messages sorted by: **[ date ]* * **[ thread ]* * **[ subject ]* * **[ author ]* ------------------------------ *In general, we found the original/classic Weigerts iron hematoxylin stain to be weak and almost washed out of tissues after staining with Massons Trichrome. We no longer use the original formula, but a more concentrated modified formulation that is not differentiated out as much by the acidifed solutions found in Mass Tri. We also found this problem with Massons Trichrome kit components, where companies probably package the original method's solutions. We make up Weigerts fresh each time, and if it will last for a week for you, fine......... but our work tends to be a one time staining with Mass Tri, then weeks before it was done again. The problem is: ferric chloride continues to oxidize the hematoxylin throughout over time, that week or longer, weakening the iron hematoxylin staining capabililty. This is discussed in Sheehan and Hrapchak Theory and Practice of Histotechnology. Acid decalcified bone presents even more of a challenge, since nuclei (DNA/RNA) in cells are hydrolyzed by acid decalcifiers, compromising staining of nuclei, a problem seen with routine H&E staining. Deanna was correct on her assessment of this stain for best results. This modified Weigerts Hematoxylin was published in J of Histotechnology in a paper on Kreybergs stain on skin. The second Extra Strength Weigerts was found on Histonet years ago and I have not tried the latter. I suggest you see which one you prefer, as we use the first Modified Weigerts. Over the years, the modified gave us far superior nuclear staining with Massons Trichrome. Weigert's Iron Hematoxyin MODIFIED (found in J of Histotechnology in a method for Kreybergs stain on mouse skin). Solution A. 2% Hematoxylin in 95% ethanol Solution B. 62% Ferric Chloride 4 ml Distilled water 95 ml Hydrochloric acid 1 ml Mix equal amounts of Solution A and Solution B MIX FRESH JUST BEFORE USE AND DISCARD AFTER USE. Extra Strength Weigerts Iron Hematoxylin from Histonet (reference unknown, but supposedly from J of Histotechnology and method originated by Mabel Myli, Mayo Clinic) Solution A: Hematoxylin 10 g 95% ethanol 100 ml Solution B: 11.6 g Ferric Chloride 980 ml Distilled water 10 ml 25% hydrochloric acid Working Stain Solution Solution A 5 ml Solution B 25 ml Absolute Ethanol 20 ml Staining time for both of these formulations is 10 minutes, followed by rinsing for 10 min in running tap water (hematoxylin will be black) Gayle M. Callis HTL/HT/MT(ASCP) ************************ You Wrote: I do Weigert's staining for 10 minutes and use it for a week at the most. I, too, have heard conflicting times about the stability of the Weigert's, but have the best results with using it no longer than a week. Deanna Rhoads HT (ASCP) * *2010/9/18 Andrea Marion **** * > > *Hi Itai, > > I am thinking that we will purchase a different Weigert's from another > company, or try one of the modified ones that were recommended on the > listserv. > > Running tap water only means that you are replacing the water the slides > are sitting in continuously, so that they have a constant supply of fresh > water. This removes any excess stain that may be bleeding from the slide, > and prevents it from recoloring the water, and restaining the tissue. > Essentially it helps pull more excess stain out of the slide. Leaving it > in tap water does not have the same effect, because the dye will reach an > equilibrium point whereby no more will be removed from the tissue. We use > a coplin jar that we stain the slides in, and simply move it to the sink > and let water run into the jar gently. You can achieve the same thing if > you do not have a sink of some sort by constantly dumping out the water > the slides are in, and replacing with clean water. It doesn't require any > special equipment, just a source of running water and whatever container > you normally stain slides in. > ** > Andrea* * * ***2010/9/18 Madary, Joseph *** *I do tap watewr because it works without wasting water and all of the water > running could bring out some chemicals that affect the subsequent staining. > Truth is most peopl;e never consider nulcei as an impolrtant step of the > masson. If you want to do a faster stain do the van Gieson.* > > * * > > *Nick Madary, HT/HTL(ASCP)QIHC*** > > *Medimmune Histology Mgr,*** > > *OMW, Area 4, Lab 2438*** > > *301.398.4745(vm)*** > > *301.398.6360(lab)*** > > *301.398.9745(fax)* > * * *2010/9/18 Jack Ratliff *** > *Dorn and Hart Microedge is now selling a Weigert's Hematoxylin kit. Maybe > you could try it out and see if your results improve. > > **www.dornandhart.com* * > ** > Jack* * * *On Sat, September 18, 2010 9:03 am, Itai Moshe wrote: > Hi, and thank's for the quick replay. > > I'm using a fresh Weigert's prepared from the sigma kit every time. > maybe it is possible to modify and to use sirius red to stain for > collage. or to use methyl green for the nuclei. > > Maye the bluing part is not done o.k, maybe like Mr' Madary offered, a > warm > tap water will do the job ? > is it that important to use running tap water, and not just to immerse the > slides in tap water, because i don't have a proper bath to do this ? > * *> Itai** * *2010/9/18 gayle callis **** * > > *You cannot use methyl green with Massons. Just don't use that Weigerts > from > the kit, make it up in house, simple. Ferric chloride goes into solution > rapidly. Then use all the other kit components. I suggest you buy > separate > kit components from Newcomer Supply or Poly Scientific. I don't know where > you are located for ordering??? You didn't say. > > Just to let you know, Massons IS NOT SPECIFIC FOR COLLAGEN, but stains all > connective tissues in a section. If you want a stain specific for > collagen, > use van Giesons. That also uses Weigerts iron hematoxylin. The sections > must be blotted after coming out of the van Giesons stain, or it washes out > in alcohols. You then blot, air dry and cover slip - although you can dip > in to a clearing agent to make the mounting media flow. Sirius red is whole > different staining method for collagen, and final viewing of that should be > with polarized light also. > > Also, collagen is very birefringent, polarized light examination even with > an H&E works. > > When you do Weigerts hemtoxylin, the running tap water wash is important, > or > you will have a mess. Find a sink > > The problem with Weigerts and Massons, is as I explained before. All the > other stains used in this method are acidified, also use phosphotungstic > acid with phosphomolybdic acid, so there is a lot of acids to differentiate > out the Weigerts hematoxylin, that is why the stronger formula for Weigerts > works. > > There is no bluing with this hematoxylin, it is not that kind of > hematoxylin, as the end result is black. BUT you must wash with tap water > for at least 10 minutes to achieve correct staining. Immersion will NOT do > the job. You would be standing there changing the water constantly. > agitating for 10 minutes in many changes of tap water. Isn't there a sink > located somewhere in your facility you can use for the hematoxylin portion > of this staining? You can carry the slides back to finish the rest of the > staining in a lab that doesn't have a sink. > > Methyl green will not work with Massons, nor any other collagen stain, it > will wash out. > > Gayle Callis > * > * > -----Original Message----- > From: * *histonet-bounces@lists.utsouthwestern.edu* > * > [mailto:**histonet-bounces@lists.utsouthwestern.edu* > *] On Behalf Of Itai Moshe > Sent: Saturday, September 18, 2010 8:03 AM > To: **deannal78@verizon.net* *; **amario3@uic.edu* > *; > * *histonet@lists.utsouthwestern.edu* *; > **MadaryJ@medimmune.com* *; **plott@uab.edu* > *; > * *atliffjack@hotmail.com* * > Subject: Re: [Histonet] Re: Masson's trichrom - problem with nuclei > staining > > Hi, and thank's for the quick replay. > > I'm using a fresh Weigert's prepared from the sigma kit every time. > maybe it is possible to modify and to use sirius red to stain for > collage. or to use methyl green for the nuclei. > > Maye the bluing part is not done o.k, maybe like Mr' Madary offered, a warm > tap water will do the job ? > is it that important to use running tap water, and not just to immerse the > slides in tap water, because i don't have a proper bath to do this ? > > Itai > > 2010/9/17 Deanna Rhoads <* *deannal78@verizon.net* > *> > > > I do Weigert's staining for 10 minutes and use it for a week at the most. > > I, too, have heard conflicting times about the stability of the > Weigert's, > > but have the best results with using it no longer than a week. > > > > Deanna Rhoads HT (ASCP) > > > > ------------------------------ > > *From:* Andrea Marion <* *amario3@uic.edu* *> > > > > *To:* **histonet@lists.utsouthwestern.edu* > * > > *Cc:* **itai.moshe@mail.huji.ac.il* * > > *Sent:* Fri, September 17, 2010 10:31:16 AM > > *Subject:* [Histonet] Re: Masson's trichrom - problem with nuclei > staining > > > > Hi Itai, > > > > I am interested to hear if you've resolved this problem. We use the same > > kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is > > similar to the one you mentioned. I cannot get nuclei staining with this > > method either. The nuclei are well stained (ie black) up to the PMA/PTA > > step, but during that step the nuclear stain is completely removed. I > > cannot shorten the PMA/PTA step without negatively effecting the collagen > > stain. > > > > I have in our original protocol that the Weigert's working solution is > > good for one month, but I cannot recall if this is from Sigma's > > specification sheet or a personal observation from a lab member. > However, > > from reading online some say it is good up to 4 months, others say it > > needs to be prepared fresh each time. I have tried fresh preparations > with > > the same results. > > > > My instinct is that something is off - the staining is just not stable > > enough to withstand the subsequent acid steps in Masson's trichrome. Can > > an expert weigh in on this? Is there a way to strengthen nuclear staining > > from Weigert's? > > > > Sigma's formulas and usage recommendations are: > > > > Part A: 1% w/v certified Hematoxylin in ethanol > > Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid > > > > Combine equal volumes Part A and Part B, stain sections for 5 minutes. > > > > > > Andrea Marion > > Graduate Student > > University of Illinois at Chicago > > amario3 /at/ uic /dot/ edu > > > > 010/9/15 Madary, Joseph <**MadaryJ@medimmune.com* > *> > > > I never use the Masson and expect decent nuclear staining. Do not forget > > to mordant in Bouins, wash it out well, and the running water or sitting > in > > several changes of warm tap water is a bluing step. > > > > > > > > Nick Madary, HT/HTL(ASCP)QIHC > > > > Histology Mgr, Medimmune > > > > 301.398.6360(lab), 4745(vm),9745(fax) > > > > 010/9/15 Patricia F Lott <* *plott@uab.edu* *> > > > Make sure when you mix solution a and solution b, you have a *very dark > > purple color* solution that smells fruity, like wine. If not, check that > > your solutions are not expired. I buy the Weigert's kit (32 oz. of Sol. > A > > and 32 oz. of Sol.B) from *Poly Scientific*, and it works very well. > > > > > > > > The purpose of the tap water is for blueing, it will make the nuclei very > > dark and crisp if you leave the whole slide rack in a sink with running > tap > > water for 10 minutes. > > > > > > > > Best Regards, > > > > Patty Lott > > > > > > 2010/9/16 Jack Ratliff <* *ratliffjack@hotmail.com* > *> > > > >> The tap water rinse is in reference to running tap water (not directly > on > >> the slide sections) while the slides sit in a make-shift water bath. > This > is > >> done over a period of time to differentiate the nuclei staining similar > to > >> a "bluing" step when working with an alum hematoxylin in a standard H&E. > >> Also keep in mind that improper fixation can also contribute to poor > nuclei > >> staining. > >> > >> Jack > >> > > > > > Itai Moshe itai.moshe <@t> * *mail.huji.ac.il* * > > Wed Sep 15 11:34:51 CDT 2010 > > > > Hi Histonet's > > > > I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with > > PFA. > > I'm using this protocol: > > **http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997* > * > > <**http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997* > *>With sigma masson's > > kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) > > The staining is beautiful, but i can't see the nuclei good enough. > > 1) Is there a way to enhance the nuclei staining ? (the nuclei is the > only > > reason that i"m not using the simpler sirius red and fast green > staining.) > > 2) What is the meaning for washing in running tap water washing, is it > done > > by putting the slides in a jar with simple tap water for a few minutes ? > > > > Thank's > > Itai > * > * * From raestask <@t> grics.net Mon Sep 20 03:54:33 2010 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Mon Sep 20 03:54:47 2010 Subject: [Histonet] Cutting Microarrays Message-ID: Good Morning All, I am having some issues when cutting a microarray containing 4mm punches. The punches are rolling and separating from the paraffin during cutting. Anyone have any tips or ideas?? Rae Staskiewicz MMCI From ree3 <@t> leicester.ac.uk Mon Sep 20 04:20:04 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon Sep 20 04:20:11 2010 Subject: [Histonet] RE: Cutting, Processing, etc In-Reply-To: <90354A475B420441B2A0396E5008D49692BF99@copc-sbs.COPC.local> References: <201009171758.o8HHw7CR085170@ame7.swcp.com> <90354A475B420441B2A0396E5008D49692BF99@copc-sbs.COPC.local> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8D956835F@EXC-MBX3.cfs.le.ac.uk> Whilst value for money and costings rule then the "management" will always look to save $$$s by hiring semi/unskilled personnel.....................the other point in this thread is that people are increasingly using the Histonet as an on-line and up to date text, which is surely not a bad thing?..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: 17 September 2010 22:09 To: james leroux Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc Hi James, I would take it a step farther with the continuing ed. I think it's beyond the supervisors it gets up into lab administration (clinical lab world). I personally know of a group of great folks that work hard and run a quality service. In the last 3 years they've had a major drop off in their continuing ed. And it, of course, is tied to the budget. Unfortunately, in this case (my view) those making the money decisions are missing the value. It seems they're unwilling to make the investment. I fear that in 5 years or less (if it continues) this service will suffer. I suspect there are other folks out there in the same boat. My hat is off to everyone out there working hard in our field and to the "enlightened" administrators and physicians that advocate continuing ed. Have a great weekend. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of james leroux Sent: Friday, September 17, 2010 10:55 AM To: 'Nails, Felton'; histotech@imagesbyhopper.com; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some "supervisors" around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 11:03 AM To: 'histotech@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?????????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention:? This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.445 / Virus Database: 271.1.1/3130 - Release Date: 09/15/10 06:34:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Mon Sep 20 04:50:52 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Sep 20 04:51:01 2010 Subject: [Histonet] Cutting Microarrays In-Reply-To: References: Message-ID: hi, this sounds like the tissue was too cool when embedded in the wax - so it has not made a nice homogenous block that cuts as one. Can u re-embed? On Mon, Sep 20, 2010 at 10:54 AM, Rae Staskiewicz wrote: > > > Good Morning All, > > > > I am having some issues when cutting a microarray containing 4mm punches. > The punches are rolling and separating from the paraffin during cutting. > Anyone have any tips or ideas?? > > > > Rae Staskiewicz > > MMCI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From hfedor <@t> jhmi.edu Mon Sep 20 07:05:23 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Sep 20 07:05:32 2010 Subject: [Histonet] Cutting Microarrays In-Reply-To: References: Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D31775AB477F@JHEMTEXVS3.win.ad.jhu.edu> Hello, We have trouble with the larger size punches as well. In order to get the cores to stick to the size try warming the block in a oven at 37 degrees for 15 minutes, put the block face down on a clean slide. (providing the melting temperature of the paraffin is about 57 degrees). After 15 minutes remove the block/slide complex and gently press down on the block, then allow the block to cool. Repeat the process 3 or 4 times. We have also had some success by sliding the block face over the heating unit of the paraffin embedder. But this only works for a few sections and then you get the same problem back. Best Regards, Helen L. Fedor Tissue Microarray Lab, Manager Prostate Tissue Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, September 20, 2010 5:51 AM To: Rae Staskiewicz; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cutting Microarrays hi, this sounds like the tissue was too cool when embedded in the wax - so it has not made a nice homogenous block that cuts as one. Can u re-embed? On Mon, Sep 20, 2010 at 10:54 AM, Rae Staskiewicz wrote: > > > Good Morning All, > > > > I am having some issues when cutting a microarray containing 4mm punches. > The punches are rolling and separating from the paraffin during cutting. > Anyone have any tips or ideas?? > > > > Rae Staskiewicz > > MMCI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Mon Sep 20 07:05:56 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Sep 20 07:06:01 2010 Subject: [Histonet] Cutting Microarrays In-Reply-To: References: Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D31775AB4781@JHEMTEXVS3.win.ad.jhu.edu> Hello, We have trouble with the larger size punches as well. In order to get the cores to stick to the size try warming the block in a oven at 37 degrees for 15 minutes, put the block face down on a clean slide. (providing the melting temperature of the paraffin is about 57 degrees). After 15 minutes remove the block/slide complex and gently press down on the block, then allow the block to cool. Repeat the process 3 or 4 times. We have also had some success by sliding the block face over the heating unit of the paraffin embedder. But this only works for a few sections and then you get the same problem back. Best Regards, Helen L. Fedor Tissue Microarray Lab, Manager Prostate Tissue Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, September 20, 2010 5:51 AM To: Rae Staskiewicz; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cutting Microarrays hi, this sounds like the tissue was too cool when embedded in the wax - so it has not made a nice homogenous block that cuts as one. Can u re-embed? On Mon, Sep 20, 2010 at 10:54 AM, Rae Staskiewicz wrote: > > > Good Morning All, > > > > I am having some issues when cutting a microarray containing 4mm punches. > The punches are rolling and separating from the paraffin during cutting. > Anyone have any tips or ideas?? > > > > Rae Staskiewicz > > MMCI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Sep 20 08:39:42 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Sep 20 08:38:56 2010 Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining In-Reply-To: <528158.30084.qm@web84308.mail.re1.yahoo.com> Message-ID: <81662272882347668D26AB207E87CF9C@lurie.northwestern.edu> We use Weigert's for 7 minutes and never more than a week either. As a precaution we keep it in the dark in a non-transparent container. That's the way I was always taught (26 years and counting) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deanna Rhoads Sent: Friday, September 17, 2010 10:08 AM To: Andrea Marion; histonet@lists.utsouthwestern.edu Cc: itai.moshe@mail.huji.ac.il Subject: Re: [Histonet] Re: Masson's trichrom - problem with nuclei staining I do Weigert's staining for 10 minutes and use it for a week at the most.? I, too, have heard conflicting times about the stability of the Weigert's, but have the best results with using it no longer than a week. Deanna Rhoads HT (ASCP) ________________________________ From: Andrea Marion To: histonet@lists.utsouthwestern.edu Cc: itai.moshe@mail.huji.ac.il Sent: Fri, September 17, 2010 10:31:16 AM Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining Hi Itai, I am interested to hear if you've resolved this problem.? We use the same kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is similar to the one you mentioned. I cannot get nuclei staining with this method either. The nuclei are well stained (ie black) up to the PMA/PTA step, but during that step the nuclear stain is completely removed. I cannot shorten the PMA/PTA step without negatively effecting the collagen stain. I have in our original protocol that the Weigert's working solution is good for one month, but I cannot recall if this is from Sigma's specification sheet or a personal observation from a lab member.? However, from reading online some say it is good up to 4 months, others say it needs to be prepared fresh each time. I have tried fresh preparations with the same results. My instinct is that something is off - the staining is just not stable enough to withstand the subsequent acid steps in Masson's trichrome. Can an expert weigh in on this? Is there a way to strengthen nuclear staining from Weigert's? Sigma's formulas and usage recommendations are: Part A: 1% w/v certified Hematoxylin in ethanol Part B: 1.2% w/v Ferric chloride, 1% v/v Hydrochloric Acid Combine equal volumes Part A and Part B, stain sections for 5 minutes. Andrea Marion Graduate Student University of Illinois at Chicago amario3 /at/ uic /dot/ edu Itai Moshe itai.moshe <@t> mail.huji.ac.il Wed Sep 15 11:34:51 CDT 2010 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 With sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a? jar with simple tap water for a few minutes ? Thank's Itai P.S By mistake I've post this before in another message with a wrong title. please respond to that message, so the title will be o.k for future archive searching. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Mon Sep 20 08:44:48 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Sep 20 08:44:30 2010 Subject: [Histonet] Posting comments In-Reply-To: References: Message-ID: Agreed. --On Saturday, September 18, 2010 1:25 PM -0400 Carrie Disbrow wrote: > > > > > > As a newbie trying to learn as much as possible, I'm disappointed when > questions are posted and the responses are sent to the individual. I > learn more when the responses are shared. Also, an archive search on the > subject will have limited information. I would think there are many in > the same boat - those who want to learn more about the craft. Even a > review of the basics is good reading for me. That is why I "reply all" > instead of "reply." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From sgoebel <@t> xbiotech.com Mon Sep 20 09:05:35 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Sep 20 09:05:42 2010 Subject: [Histonet] How long ... Message-ID: <20100920070535.9e2d9aa830e8449a2412eb1e4f2f067e.270b4bd9c8.wbe@email04.secureserver.net> First off you shouldn't leave Bouin's sitting for that long out o shelf. Once the water evaporates out of the solution it is extrem ely flammable (explosive even I think?). Also, you shouldn't leave ti jerky" su make up an it if it is probably just chalk it u Luck...let me know what happens!! Sarah Histotechnician XBio 8201 East Riverside Dr. Bldg 4 Suite 100< Austin, Texas 78744 < < -------- Original Message -------- Subject: [Histonet] How long ... From: Massimo <[1]max_histo_00@yah Date: Sun, September 19, 2010 3:41 pm To: [2]histonet@lists.uts Cc: [3]histonet@lists.uts Hi all, I found into a shelf of the laboratory a small flask containing mouse sampl fixed in Bouin's fluid and preserved in ethanol at 70?, forgotten ther few years. I wonder if it would be possible to continue the process up to paraffin embedding for histological preparations. Some time ago a professor of biology at the University of Florence told me they could remain in alcohol for years, but on literature I found that the can not be so long without altering tissues. Has anyone had a similar experience? It's better to throw everything away or not? Thank you in advance. Kind Regards, Massimo Tosi _______________________________________________ Histonet mailing list [4]Histonet@lists.utsouth [5]http: References 1. 3D"mailto:max_histo_00@yahoo.it" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:Histonet@lists.utsouthwestern.edu" 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From joycefr <@t> frontiernet.net Mon Sep 20 09:15:49 2010 From: joycefr <@t> frontiernet.net (Joyce Friedland) Date: Mon Sep 20 09:09:58 2010 Subject: [Histonet] Job Opening Message-ID: <5D037249-F518-4284-9218-6F48B6307C36@frontiernet.net> Hi All, Our private derm-path lab, located in central-western New York State, has an immediate opening for a full time histotech to work days. A relocation bonus is negotiable. To learn more please contact me directly. Joyce From nancy_schmitt <@t> pa-ucl.com Mon Sep 20 10:00:41 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Sep 20 10:00:59 2010 Subject: [Histonet] RE: Histonet Digest, Vol 82, Issue 26 In-Reply-To: <20100919170422.D349F1559E8@mail.pa-ucl.com> References: <20100919170422.D349F1559E8@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC06443B280B55D@hestia.ad.pa-ucl.com> We have three CBG recyclers - two are used for formalin and one is used for alcohol/xylene. They run every day and we have very little trouble with them. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA ------------------------------ Message: 6 Date: Sat, 18 Sep 2010 20:45:52 -0700 From: Melinda Sokol Subject: [Histonet] CBG Recycler To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi All I would like Pros and Cons regarding a CBG Recycler. Please- No Vendors. Thanks Melinda A Hamilton HT (ASCP) ------------------------------ NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From GDawson <@t> dynacaremilwaukee.com Mon Sep 20 10:04:57 2010 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Sep 20 10:05:02 2010 Subject: FW: [Histonet] Posting comments Message-ID: -----Original Message----- From: Dawson, Glen Sent: Monday, September 20, 2010 9:10 AM To: 'Merced M Leiker' Subject: RE: [Histonet] Posting comments Carrie, Until you've had some psychopath threaten you with a lawsuit, or threaten to "tell on you" with your current employer, it will be difficult to understand why most of us choose to reply directly to whomever posts the question. I've had this happen to me on this list because "someone" out their did not agree with my general post & needed to act like a child to get his point across. I've responded directly to the person that posts the question ever since...basically to avoid the hassle. I realize that this isn't the best situation for the distribution of useful information, but all of us need to remember that there will always be a small percentage of bad apples in the basket that is the histonet and that those of us that won't hit "reply to all" don't do so of our own accord. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Monday, September 20, 2010 8:45 AM To: Carrie Disbrow; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Posting comments Agreed. * On Saturday, September 18, 2010 1:25 PM -0400 Carrie Disbrow wrote: > > > > > > As a newbie trying to learn as much as possible, I'm disappointed when > questions are posted and the responses are sent to the individual. I > learn more when the responses are shared. Also, an archive search on the > subject will have limited information. I would think there are many in > the same boat - those who want to learn more about the craft. Even a > review of the basics is good reading for me. That is why I "reply all" > instead of "reply." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ctorrence <@t> kmcpa.com Mon Sep 20 10:11:20 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Mon Sep 20 10:11:36 2010 Subject: [Histonet] Out of Office Reply Message-ID: <88abe6cf75594ade9f25a97274b65756@043bd27da50a440e9ee2cd7149ffef06> I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From arsenn <@t> hsh.org Mon Sep 20 10:19:08 2010 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Mon Sep 20 10:19:31 2010 Subject: [Histonet] Questions Message-ID: I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with "oh no" or "oh my gosh" - I feel as though I should apologize for my "stupid" question..... However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. From arsenn <@t> hsh.org Mon Sep 20 10:25:10 2010 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Mon Sep 20 10:25:30 2010 Subject: [Histonet] (no subject) Message-ID: James, Well said. I totally agree!!!! Thank you very much! Amy Message: 4 Date: Fri, 17 Sep 2010 11:54:42 -0600 From: "james leroux" Subject: RE: [Histonet] RE: Cutting, Processing, etc To: "'Nails, Felton'" , , "'mohamed abd el razik'" , Message-ID: <201009171758.o8HHw7CR085170@ame7.swcp.com> Content-Type: text/plain; charset="iso-8859-1" Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some "supervisors" around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 Joyce, As always, your 2 cents is 'right on'! Thank you! Amy Message: 2 Date: Fri, 17 Sep 2010 13:07:16 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] RE: Cutting, Processing, etc To: "Nails, Felton" , "'histotech@imagesbyhopper.com'" , 'mohamed abd el razik' , "Histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403984C06EB@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" My 2 cents is that she needed to convince someone this was how it is done! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. From POWELL_SA <@t> mercer.edu Mon Sep 20 10:32:35 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Sep 20 10:32:42 2010 Subject: [Histonet] RE: Questions In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE226901FD127@MERCERMAIL.MercerU.local> Amy, You do not need to apologize for asking a question to which you did not know the answer. This is an educational avenue, for histology, and there is no such thing as a "stupid" question if you need answers to solve a problem. Those of us who teach know questions are important, even if you think you know the answer but not exactly sure, or in your case you knew but needed documented verification from others in the field. I hope your fellow workers and supervisors got the message and please feel free to ask. There are those in the field who feel this is a social network for experts and that is okay too, but the real reason NSH and histosearch was started was to expand knowledge of the histology community and to improve our profession. Remembering when histology was in the basement and no one knew we were there, it makes me proud of the progress we have made in the 48 years I have been in the field. Keep asking and share what you know, no need for apologies. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, September 20, 2010 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Questions I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with "oh no" or "oh my gosh" - I feel as though I should apologize for my "stupid" question..... However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From higginst <@t> amapath.com Mon Sep 20 10:41:43 2010 From: higginst <@t> amapath.com (Tim Higgins) Date: Mon Sep 20 10:45:54 2010 Subject: [Histonet] RE: Questions In-Reply-To: <9BF995BC0E47744E9673A41486E24EE226901FD127@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE226901FD127@MERCERMAIL.MercerU.local> Message-ID: <000201cb58da$5450fe20$6a03a8c0@apg> Agreed! Thanks, Timothy Higgins, HT(ASCP) QIHC Histology Manager APA Amarillo, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, September 20, 2010 10:33 AM To: Senn, Amy R; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Questions Amy, You do not need to apologize for asking a question to which you did not know the answer. This is an educational avenue, for histology, and there is no such thing as a "stupid" question if you need answers to solve a problem. Those of us who teach know questions are important, even if you think you know the answer but not exactly sure, or in your case you knew but needed documented verification from others in the field. I hope your fellow workers and supervisors got the message and please feel free to ask. There are those in the field who feel this is a social network for experts and that is okay too, but the real reason NSH and histosearch was started was to expand knowledge of the histology community and to improve our profession. Remembering when histology was in the basement and no one knew we were there, it makes me proud of the progress we have made in the 48 years I have been in the field. Keep asking and share what you know, no need for apologies. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, September 20, 2010 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Questions I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with "oh no" or "oh my gosh" - I feel as though I should apologize for my "stupid" question..... However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From klauing <@t> lumc.edu Mon Sep 20 11:05:45 2010 From: klauing <@t> lumc.edu (Kristen Lauing) Date: Mon Sep 20 11:06:12 2010 Subject: [Histonet] RE: Questions Message-ID: <4C974009020000FD0001F382@gwgwia2.luhs.org> Shirley and Amy and others responding to this thread: thanks for your posts. I am not in the histotechnology field - I'm actually a graduate student at Loyola who does her own processing, cutting, and staining of bone tissue - so I really appreciate every so-called "dumb question", no matter how simple, since I have never been formally trained in the field. I assumed this was a great forum to safely ask those kinds of questions to advance my graduate student research. I'm learning new details about histology every day just by reading posts by helpful people like you. Thanks & have a great week, Kristen >>> "Shirley A. Powell" 09/20/10 10:35 AM >>> Amy, You do not need to apologize for asking a question to which you did not know the answer. This is an educational avenue, for histology, and there is no such thing as a "stupid" question if you need answers to solve a problem. Those of us who teach know questions are important, even if you think you know the answer but not exactly sure, or in your case you knew but needed documented verification from others in the field. I hope your fellow workers and supervisors got the message and please feel free to ask. There are those in the field who feel this is a social network for experts and that is okay too, but the real reason NSH and histosearch was started was to expand knowledge of the histology community and to improve our profession. Remembering when histology was in the basement and no one knew we were there, it makes me proud of the progress we have made in the 48 years I have been in the field. Keep asking and share what you know, no need for apologies. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, September 20, 2010 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Questions I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with "oh no" or "oh my gosh" - I feel as though I should apologize for my "stupid" question..... However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Mon Sep 20 11:09:41 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Mon Sep 20 11:09:51 2010 Subject: [Histonet] RE: Questions In-Reply-To: <9BF995BC0E47744E9673A41486E24EE226901FD127@MERCERMAIL.MercerU.local> Message-ID: Well said, Shirley! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Shirley A. Powell" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/20/2010 11:37 AM To "Senn, Amy R" , "Histonet@lists.utsouthwestern.edu" cc Subject [Histonet] RE: Questions Amy, You do not need to apologize for asking a question to which you did not know the answer. This is an educational avenue, for histology, and there is no such thing as a "stupid" question if you need answers to solve a problem. Those of us who teach know questions are important, even if you think you know the answer but not exactly sure, or in your case you knew but needed documented verification from others in the field. I hope your fellow workers and supervisors got the message and please feel free to ask. There are those in the field who feel this is a social network for experts and that is okay too, but the real reason NSH and histosearch was started was to expand knowledge of the histology community and to improve our profession. Remembering when histology was in the basement and no one knew we were there, it makes me proud of the progress we have made in the 48 years I have been in the field. Keep asking and share what you know, no need for apologies. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, September 20, 2010 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Questions I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with "oh no" or "oh my gosh" - I feel as though I should apologize for my "stupid" question..... However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Cathy.Crumpton <@t> tuality.org Mon Sep 20 12:05:49 2010 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Mon Sep 20 12:05:54 2010 Subject: [Histonet] Skills testing for a histo lab assistant Message-ID: We are going to be posting a histology lab assistant position soo for the first time. I would like to give them a skills assessment t est to see how well they can handle a computer, numbers, problem solving, a there do this f &nb Cathy Crumpton HT(ASCP), Histology Lead Tuality Community Hillsboro, OR 97123 (503)681-1292 From histo20 <@t> hotmail.com Mon Sep 20 12:11:46 2010 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Mon Sep 20 12:11:52 2010 Subject: [Histonet] multi-timer Message-ID: Hi everybody! Does anyone know where I can purchase a multi-timer (product used to be manufactured by Beckman Coulter electronics)? The company no longer manufactures them. Thank you so much! Paula Wilder St. Joseph Medical Center 410-337-1741 From MLashus <@t> pathgroup.com Mon Sep 20 12:16:52 2010 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Mon Sep 20 12:16:57 2010 Subject: [Histonet] multi-timer In-Reply-To: References: Message-ID: <197CD0B02A81F94994A285C59C8AE05C05E84AEC85@pgnexchange.pathgroup.com> Try Labsco. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Wilder Sent: Monday, September 20, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] multi-timer Hi everybody! Does anyone know where I can purchase a multi-timer (product used to be manufactured by Beckman Coulter electronics)? The company no longer manufactures them. Thank you so much! Paula Wilder St. Joseph Medical Center 410-337-1741 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From Luisa.Onstead-Cardinale <@t> jax.ufl.edu Mon Sep 20 14:18:10 2010 From: Luisa.Onstead-Cardinale <@t> jax.ufl.edu (Onstead-Cardinale, Luisa) Date: Mon Sep 20 14:18:48 2010 Subject: [Histonet] Steedman polyester wax Message-ID: I would like to know if anyone has a good protocol that includes tissue fixation (preferred fixative, fixation temperature etc) and tissue processing (time etc.) for low melting Steedman polyester wax . The tissue to be embedded is chick embryo brain. I am going to do all the tissue processing and embedding manually. Any suggestion? Looking forward to receive your advice Luisa Luisa Onstead-Cardinale, M.S. Research Associate UF Biomedical Research Laboratory, Room 121 655 West 11th Street Jacksonville, Fl 32206 phone (904) 633-0984 From gankam <@t> googlemail.com Mon Sep 20 17:49:55 2010 From: gankam <@t> googlemail.com (Fabrice GANKAM) Date: Mon Sep 20 17:50:45 2010 Subject: [Histonet] multi-timer In-Reply-To: References: Message-ID: Hey guys Was wondering if any of you ever used a phopho NFKB p65 antibodu and which one you prefer with what antigen retrieval method if applies. I'm working on FFPE rat tissue Thanks. Fabrice From gankam <@t> googlemail.com Mon Sep 20 17:49:55 2010 From: gankam <@t> googlemail.com (Fabrice GANKAM) Date: Mon Sep 20 17:58:16 2010 Subject: [Histonet] multi-timer In-Reply-To: References: Message-ID: Hey guys Was wondering if any of you ever used a phopho NFKB p65 antibodu and which one you prefer with what antigen retrieval method if applies. I'm working on FFPE rat tissue Thanks. Fabrice From AGleiberman <@t> cbiolabs.com Tue Sep 21 09:09:27 2010 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue Sep 21 09:09:37 2010 Subject: [Histonet] NFkB p65 In-Reply-To: References: Message-ID: Hi Fabrice, If you are looking for NFkB activation I suggest to use antibody against non-phosphorylated p65 (rabbit from Abcam, CHIP grade) and watch nuclear translocation of p65. Different anti-phospho-p65 did not give me anything, while anti-p65 clearly shows NFkB activation every time (and it correlates very well with biochemical data). The best results I got was with fresh-frozen sections (with subsequent formaldehyde fixation), next - cryo sections from formaldehyde perfused mice or rats, next - cryo from formaldehyde fixed tissues, and finally - sometimes got some positive results from FFPE after retrieval by citrate boiling. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fabrice GANKAM Sent: Monday, September 20, 2010 6:50 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] multi-timer Hey guys Was wondering if any of you ever used a phopho NFKB p65 antibodu and which one you prefer with what antigen retrieval method if applies. I'm working on FFPE rat tissue Thanks. Fabrice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From alyssa <@t> alliedsearchpartners.com Tue Sep 21 09:50:26 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Sep 21 09:50:38 2010 Subject: [Histonet] Correction To OH Position-Now Direct/Permanent Hire Message-ID: Allied Search Partners has been retained to search for a Histotechnician or Histotechnologist for Permanent Direct Hire. The physician is looking for a qualified candidate to work ASAP. Location of Laboratory: Toledo, OH (local candidates please) Environment: Multi-specialty clinic serving the community with 108 physicians and 37 specialties. Shift: Full Time, Monday-Friday from 8am-5pm Department: Dermasurgery Requirements: HT ASCP Prior MOHS experienced preferred CLIA eligible to gross Job Duties: Grossing Assist physician Make permanent sections MOHS Histology Other related duties Other: To schedule a phone interview with one of our recruiters please submit resume to Alyssa@alliedsearchpartners.com -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From cfrmd1 <@t> gmail.com Tue Sep 21 10:07:05 2010 From: cfrmd1 <@t> gmail.com (Carlos Rodriguez, MD) Date: Tue Sep 21 10:07:09 2010 Subject: [Histonet] Melanocytic IHC kits In-Reply-To: References: Message-ID: Hi all: can anyone who has experience using ihc kits in a smaller lab setting chime in on which kits they prefer from a quality and ease of use standpoint? Mainly melanocytic ihc would be needed including S100, melan a, mitf, tyrosinase. Thanks and have a great day. From kadamsplw <@t> gmail.com Tue Sep 21 10:12:24 2010 From: kadamsplw <@t> gmail.com (karen adams) Date: Tue Sep 21 10:12:53 2010 Subject: [Histonet] Freezer alarm Message-ID: Good Morning.......... Can anyone make a recommendation for an alrm system that will call me if the power goes out to our freezer??? Thanks! -- From ctorrence <@t> kmcpa.com Tue Sep 21 12:08:48 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Tue Sep 21 12:09:04 2010 Subject: [Histonet] Out of Office Reply Message-ID: <173d745005244864bbbb90da64159ed0@7f11dbd5158c4ee98c23ffead185044e> I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From srodriguez <@t> phenopath.com Tue Sep 21 13:35:17 2010 From: srodriguez <@t> phenopath.com (Stephanie Rodriguez) Date: Tue Sep 21 13:35:36 2010 Subject: [Histonet] Re: Histonet Digest, Vol 82, Issue 29 Message-ID: We use ADT. I don?t know where you?re located, so I don?t know if that?s an option for you, but we?ve used them for at least 8 years, and they?ve always been excellent. Stephanie Stephanie Rodriguez, HTL(ASCP), QIHC Lead Molecular Technologist-FISH IHC Technologist III Phenopath Laboratories Seattle, WA (206) 374-9000 On 9/21/10 10:06 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > Message: 10 > Date: Tue, 21 Sep 2010 11:12:24 -0400 > From: karen adams > Subject: [Histonet] Freezer alarm > To: Histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Good Morning.......... > > Can anyone make a recommendation for an alrm system that will call me > if the power goes out to our freezer??? > > > Thanks! This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Carol.Wilson <@t> ricerca.com Tue Sep 21 13:58:53 2010 From: Carol.Wilson <@t> ricerca.com (Wilson, Carol) Date: Tue Sep 21 13:59:05 2010 Subject: [Histonet] GGT IHC in NHP Message-ID: <29E866ED01CCE24093EF98B1F660136B45A9F0@Redwood.ricerca.com> Hi All, Does anyone out there have experience staining for GGT in NHP tissue, specifically Rhesus monkey? If so, could you please forward any information you may have. I was told by a business manager that this is "just a standard special stain" , like an iron, PAS or Trichrome, and should be able to accommodate within our standard histology offerings. Thanks in advance for all help. Carol Carol Wilson, HT(ASCP) Lead Technician/Histology Ricerca Biosciences, LLC From a.craig.mackinnon <@t> gmail.com Tue Sep 21 15:56:16 2010 From: a.craig.mackinnon <@t> gmail.com (craig mackinnon) Date: Tue Sep 21 15:56:21 2010 Subject: [Histonet] Please post JOB POSITION: MILWAUKEE WI Message-ID: PURPOSE OF POSITION Perform a variety of routine and special histological and immunohistochemical staining procedures to support clinical and translational research activities in a new core lab at MCW. Develop and implements new techniques. ESSENTIAL DUTIES 1. Direct daily operations of all phases of histology and immunohistochemistry of the Clincal and Translational Research Core Lab 2. Train and supervise histotechnologists and core lab users 3. Develop, write, and validate histology and immunohistochemistry protocols 4. Develop and introduce new methodologies and techniques to support client users 5. Operate and maintain histology equipment, perform/supervise repairs, update computer hardware and software 6. Responsible for managing, maintaining, tracking, and stocking supplies 7. Assist with fiscal budgeting, capital equipment forecasting, purchasing 8. Responsible for safety issues regarding biohazardous materials and histology equipment 9. Participate in regularly scheduled meetings to evaluate data, methodologies, and procedures to establish and maintain a quality assurance program 10. Handle compliance issues 11. Responsible for meeting regulatory requirements (eg, CAP, CLIA 88) 12. Assist with outreach programs, grant writings, presentations, and manuscript preparations Contact: Dr. Craig Mackinnon, MD, PhD a.craig.mackinnon@gmail.com From Luisa.Onstead-Cardinale <@t> jax.ufl.edu Wed Sep 22 07:46:12 2010 From: Luisa.Onstead-Cardinale <@t> jax.ufl.edu (Onstead-Cardinale, Luisa) Date: Wed Sep 22 07:46:31 2010 Subject: [Histonet] hematoxylin stain for polyester wax sections Message-ID: Can anyone suggest a good hematoxylin counterstain for central nervous system tissue embedded in polyester wax? And provide a recipe? Thank you Luisa Luisa Onstead-Cardinale, M.S. Research Associate UF Biomedical Research Laboratory, Room 121 655 West 11th Street Jacksonville, Fl 32206 phone (904) 633-0984 From leiker <@t> buffalo.edu Wed Sep 22 08:45:09 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Sep 22 08:45:17 2010 Subject: [Histonet] Re: Histonet Digest, Vol 82, Issue 29 In-Reply-To: References: Message-ID: <71D7A03012D5563B801499E0@CDYwxp1931.ad.med.buffalo.edu> We've used DSC (http://www.dsc.com/) since Feb 2007 and it's worked nicely. We have it tied in to our institution security force which calls us off-hours in case a freezer or incubator stops working. --On Tuesday, September 21, 2010 11:35 AM -0700 Stephanie Rodriguez wrote: > We use ADT. I don?t know where you?re located, so I don?t know if > that?s an option for you, but we?ve used them for at least 8 years, and > they?ve always been excellent. > > Stephanie > > > Stephanie Rodriguez, HTL(ASCP), QIHC > Lead Molecular Technologist-FISH > IHC Technologist III > Phenopath Laboratories > Seattle, WA > (206) 374-9000 > > > > > On 9/21/10 10:06 AM, "histonet-request@lists.utsouthwestern.edu" > wrote: > >> Message: 10 >> Date: Tue, 21 Sep 2010 11:12:24 -0400 >> From: karen adams >> Subject: [Histonet] Freezer alarm >> To: Histonet@lists.utsouthwestern.edu >> Message-ID: >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Good Morning.......... >> >> Can anyone make a recommendation for an alrm system that will call >> me if the power goes out to our freezer??? >> >> >> Thanks! > > > > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call > PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From flnails <@t> texaschildrens.org Wed Sep 22 09:53:25 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Sep 22 09:53:41 2010 Subject: [Histonet] Freezer alarm In-Reply-To: References: Message-ID: All of our freezers and refrigerators are hard wired and monitored by our security department and they call the responsible parties in the event that the temperature drops below a set temperature -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karen adams Sent: Tuesday, September 21, 2010 10:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezer alarm Good Morning.......... Can anyone make a recommendation for an alrm system that will call me if the power goes out to our freezer??? Thanks! -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From MW <@t> PersonifySearch.com Wed Sep 22 09:55:20 2010 From: MW <@t> PersonifySearch.com (Matthew Ward) Date: Wed Sep 22 09:55:25 2010 Subject: [Histonet] Histology Training Specialist Opportunity with a Global Leader! Message-ID: <001b01cb5a66$2db8f050$892ad0f0$@com> Good Morning, Our firm is currently partnered with a global leader in Histology that is going through a large expansion. We currently are searching for histology professionals who would be interested in working in house providing support and training to customers, clients, and current employees. Please contact me directly to learn more! Histology Training Specialist The Company: Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. The Opportunity: The company currently has an opening for a Histology Training Specialist. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: $55-60k+ Other: Full benefits - 401k program/matching Located in the Chicago area. Primary Responsibilities: The primary responsibility of this role will be to provide technical phone support by answering questions, troubleshooting problems, logging and closing complaint files and escalating major issues to appropriate company personnel. This role will also provide technical training on specified products in the company's newly constructed state-of-the-art Customer Support Laboratory. Training programs are designed for small groups to ensure maximum customer learning and satisfaction. Additional Responsibilities: - Provide product and applications phone support to end-users, field personnel and dealers for all product lines - Log all calls into Customer Support Database - Participate in development of training materials and conduct classes and labs for customers, employees and others as needed - Serve as technical liaison to Customer Service/Field Service/Product Management departments Education and Experience Required: Ability to interact with various people in a calm and positive fashion and the ability to effectively communicate information to groups of participants is required. Experience with data entry, MS Office programs (PowerPoint, Lotus Notes, Word) is also required. HT/HTL/QIHC (ASCP) is helpful but not required. Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From sbaldwin <@t> mhhcc.org Wed Sep 22 10:56:20 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Sep 22 10:57:21 2010 Subject: [Histonet] FORMALIN DISPOSAL Message-ID: Histonetters: Our disposal company for formalin wants us to separate our stored tissues from the formalin and then pour the formalin into a drum. Does anyone have a procedure that doesn't expose your histotechs when pouring formalin off stored tissues, the company states there isn't a container that we can use to put the formalin and tissues in without being non-complient. Does anyone have a suggestion? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From laurie.colbert <@t> huntingtonhospital.com Wed Sep 22 11:48:02 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Sep 22 11:48:06 2010 Subject: [Histonet] FORMALIN DISPOSAL In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D7683098F02F1@EXCHANGE3.huntingtonhospital.com> Kathy, I'm not sure where you're located, but I am in California and we use a disposal company called Stericycle. They separate the waste for us and take both the specimens and formalin. I believe Stericycle works in other states besides California. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Wednesday, September 22, 2010 8:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FORMALIN DISPOSAL Histonetters: Our disposal company for formalin wants us to separate our stored tissues from the formalin and then pour the formalin into a drum. Does anyone have a procedure that doesn't expose your histotechs when pouring formalin off stored tissues, the company states there isn't a container that we can use to put the formalin and tissues in without being non-complient. Does anyone have a suggestion? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Sep 22 12:00:05 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Sep 22 12:00:13 2010 Subject: [Histonet] FORMALIN DISPOSAL In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5DF8388@LRGHEXVS1.practice.lrgh.org> Our gross room is set up a with a fume hood that runs the entire length of one wall, this includes our sink. When we need to dump tissue, we plug the drain then lay down our formalin pads to collect any spills. We place a large specimen container on the pads add a large screen then pour off the formalin into the container. We use another container to collect the various parts and when full we cap it then place in the waste box that gets incinerated. The waste formalin is then de-formalized (Surgipath product), aldehyde and ph are check and if they pass we can then drain dispose. If not, we collect it as waste which is then stored in drums to be hauled away. This procedure was approved by our state DES. If you cannot dump the tissue under a hood like this, you will need to wear a chemical respirator, all of my staff have their own full face respirators and have been fit tested for them. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Wednesday, September 22, 2010 11:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FORMALIN DISPOSAL Histonetters: Our disposal company for formalin wants us to separate our stored tissues from the formalin and then pour the formalin into a drum. Does anyone have a procedure that doesn't expose your histotechs when pouring formalin off stored tissues, the company states there isn't a container that we can use to put the formalin and tissues in without being non-complient. Does anyone have a suggestion? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From ctorrence <@t> kmcpa.com Wed Sep 22 12:07:00 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Wed Sep 22 12:07:16 2010 Subject: [Histonet] Out of Office Reply Message-ID: <8c2cca8a5c724da7b3b2036599c6b1e1@c797310549a24c5db6e1c0abfaa9b358> I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From Fawn.Bomar <@t> HalifaxRegional.com Wed Sep 22 14:06:58 2010 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Wed Sep 22 14:07:23 2010 Subject: [Histonet] Her2 question Message-ID: Hi everyone, I was wondering how many of you all use the Cell Marque Her2 predilute? We have been using it at our facility for 2 years now and it has been great until we received a new lot. This new lot does not seem to be staining as well and I was wondering if anyone else was having the same problem and if they managed to get it to stain better. I called Cell Marque and they told me that they optimized the antibody again so it is different from what we have been using. I am adjusting a few things on our protocol to see if it works and I was wondering if anyone had any tips. We use the BenchMark LT and our protocol is as follows: Deparaffinization, Standard CC1, Antibody incubation 32 minutes (the maximum that the machine allows), A/B Block, Hematoxylin-6 minutes, and bluing-4 minutes. Thank you Fawn Bomar HT(ASCP) ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From Leiferman <@t> ortho.wisc.edu Wed Sep 22 14:16:35 2010 From: Leiferman <@t> ortho.wisc.edu (Leiferman, Ellen) Date: Wed Sep 22 14:16:41 2010 Subject: [Histonet] Technovit 9100 Destabilizing Basic Solution Message-ID: Hello, Has anyone had success at filtering the Basic Solution of the Technovit 9100 kit? Our lab is interested in embedding mineralized scaffolds in PMMA. Since the scaffold is mineralized, decided to go PMMA route although temperature is a concern so we purchased the Technovit 9100 kit. One of the first steps in kit requires you to destabilize the Basic Solution by passing it through a column of aluminum oxide. The directions that came with the kit suggested a chromatography column------we used a Sephadex column and loaded with 20 grams of aluminum oxide followed by the addition of Basic Solution. The gravity flow rate was very slow, probably obtained 10 ml over a few hours of operation. I noted in one of the papers a research lab used 20 grams aluminum oxide in a 60 cc syringe along with millipore .2 micrometer filter attached. My thinking was that maybe by being able to push the fluid through the "syringe& filter set-up" it may go faster than plain gravity feed. I was wrong as this set-up clogged up immediately and I was unable to push anything out. If anyone has had success at filtering the basic solution and wouldn't mind sharing their technique I would be most appreciative. Kind Regards, Ellen Leiferman From JMitchell <@t> uwhealth.org Wed Sep 22 14:30:48 2010 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Wed Sep 22 14:30:51 2010 Subject: [Histonet] Lysine Buffer Message-ID: <2108AECB05DBFF48A9C436A79215574002D2EBBB@UWHC-MAIL03.uwhis.hosp.wisc.edu> I use minimal quantities of lysine buffer in PLP fixative and my protocol notes a shelf life of one month for the buffer solution. Instead of making up so often has anyone frozen lysine buffer in small quantities and thawed prior to use? Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 From sbaldwin <@t> mhhcc.org Wed Sep 22 14:32:11 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Sep 22 14:33:40 2010 Subject: [Histonet] HER 2 Message-ID: Which detection kit do you use? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From Fawn.Bomar <@t> HalifaxRegional.com Wed Sep 22 14:40:50 2010 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Wed Sep 22 14:41:30 2010 Subject: [Histonet] HER 2 In-Reply-To: References: Message-ID: I-VIEW DAB Detection Kit from Ventena ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org [sbaldwin@mhhcc.org] Sent: Wednesday, September 22, 2010 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER 2 Which detection kit do you use? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From dprieto <@t> fcien.edu.uy Wed Sep 22 15:15:39 2010 From: dprieto <@t> fcien.edu.uy (Daniel Prieto) Date: Wed Sep 22 15:16:10 2010 Subject: [Histonet] FORMALIN DISPOSAL In-Reply-To: References: Message-ID: <4C9A63EB.6070506@fcien.edu.uy> You could try oxidizing formalin with 5% sodium hypochlorite to CO2 and formic acid, which in turn can be neutralized with sodium bicarbonate and poured down the drain. This can be performed under a regular fume hood which I assume is something you cannot do with a large drum. Of course disposal of tissues should be checked for compatibility with local regulations. cheers, Daniel ------------------------------------- Daniel Prieto Laboratorio de Cultivo de Tejidos Departamento de Biologia Celular y Molecular Facultad de Ciencias - Universidad de la Republica Uruguay El 22/09/10 12:56, Sara Baldwin/mhhcc.org escribi?: > Histonetters: > Our disposal company for formalin wants us to separate our stored tissues from the formalin and then pour the formalin into a drum. > Does anyone have a procedure that doesn't expose your histotechs when pouring formalin off stored tissues, the company states there isn't a container that we can use to put the formalin and tissues in without being non-complient. Does anyone have a suggestion? > > Thanks > Pathology Supervisor > Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-482-0210, 482-0216, Fax 812-482-0232, > Pager 812-481-0897 > Confidential information, Authorized use only. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From syinan <@t> ucalgary.ca Wed Sep 22 16:39:20 2010 From: syinan <@t> ucalgary.ca (Salim Yalcin Inan) Date: Wed Sep 22 16:39:38 2010 Subject: [Histonet] post-fixation question Message-ID: Hello, I have a question about post-fixation. As far as I know, for most of the immunohistochemistry studies in mouse/rat brain, post-fixation period is between 2 hours to 24 hours in 4% paraformaldehyde at +4 Celsius followed by cryoprotection with 20-30% sucrose solution. Are there any disadvantages to post-fix the tissues for more than 24 hours (2 days-1 week) for further immunohistochemistry experiments? Thank you very much in advance. Sincerely Yours, Salim Inan, PhD HBI, University of Calgary From Lesley.Smith <@t> ramsayhealth.co.uk Thu Sep 23 02:27:34 2010 From: Lesley.Smith <@t> ramsayhealth.co.uk (Smith, Lesley) Date: Thu Sep 23 02:28:04 2010 Subject: [Histonet] Leica XL autostainer wash pots Message-ID: <37ACDF51E6FCB146B2D92527B733EB35027146A8@uksbirs-ukmg01.ukramsay.rhc.local> We have just acquired a Leica XL Autostainer. However Leica have told us that the wash pots have been discontinued (and we need 5 of them!!) Does anyone have an unused XL gathering dust somewhere that we could recycle your wash pots! Fingers crossed and many thanks. Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk ************************************************ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. From jsjurczak <@t> comcast.net Thu Sep 23 08:17:14 2010 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Thu Sep 23 08:17:19 2010 Subject: [Histonet] Leica XL autostainer wash pots In-Reply-To: <37ACDF51E6FCB146B2D92527B733EB35027146A8@uksbirs-ukmg01.ukramsay.rhc.local> Message-ID: <1191565807.1794400.1285247834923.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Try Tech One or Marston, they are service companies and might have some. I can't believe Leica would discontinue parts for the XL. Are you sure this isn't just a temporary situation? That would be so unlike Leica to make things difficult for us..... ----- Original Message ----- From: "Lesley Smith" To: histonet@lists.utsouthwestern.edu Sent: Thursday, September 23, 2010 2:27:34 AM Subject: [Histonet] Leica XL autostainer wash pots We have just acquired a Leica XL Autostainer. However Leica have told us that the wash pots have been discontinued (and we need 5 of them!!) Does anyone have an unused XL gathering dust somewhere that we could recycle your wash pots! Fingers crossed and many thanks. ? Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW ? Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk ************************************************ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Thu Sep 23 08:35:14 2010 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Thu Sep 23 08:35:23 2010 Subject: [Histonet] post-fixation question In-Reply-To: References: Message-ID: The longer the tissue sits in paraformaldehyde the more the proteins in the tissues will become cross-linked due to multiple methylene bridge formations and could require very rigorous antigen retrieval or may even be beyond retrieval; the tissues could become brittle as well due to over-fixation. Just my 2 cents. Regards, Merced --On Wednesday, September 22, 2010 3:39 PM -0600 Salim Yalcin Inan wrote: > Hello, > > > > I have a question about post-fixation. As far as I know, for most of the > immunohistochemistry studies in mouse/rat brain, post-fixation period is > between 2 hours to 24 hours in 4% paraformaldehyde at +4 Celsius followed > by cryoprotection with 20-30% sucrose solution. Are there any > disadvantages to post-fix the tissues for more than 24 hours (2 days-1 > week) for further immunohistochemistry experiments? Thank you very much > in advance. > > Sincerely Yours, > > Salim Inan, PhD > > HBI, University of Calgary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From mcauliff <@t> umdnj.edu Thu Sep 23 08:58:49 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Sep 23 08:56:02 2010 Subject: [Histonet] post-fixation question In-Reply-To: References: Message-ID: <4C9B5D19.1000108@umdnj.edu> Merced is correct about longer fixation inducing more cross-linking, possibly making immunostaining more difficult. Much depends on the antigen you are looking for. I disagree that the tissue will become brittle, this is not my experience. Geoff Salim Yalcin Inan wrote: > Hello, > > > > I have a question about post-fixation. As far as I know, for most of the > immunohistochemistry studies in mouse/rat brain, post-fixation period is > between 2 hours to 24 hours in 4% paraformaldehyde at +4 Celsius followed by > cryoprotection with 20-30% sucrose solution. Are there any disadvantages to > post-fix the tissues for more than 24 hours (2 days-1 week) for further > immunohistochemistry experiments? Thank you very much in advance. > > Sincerely Yours, > > Salim Inan, PhD > > HBI, University of Calgary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From MSHERWOOD <@t> PARTNERS.ORG Thu Sep 23 09:02:38 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Sep 23 09:02:43 2010 Subject: [Histonet] Leica XL autostainer wash pots In-Reply-To: <1191565807.1794400.1285247834923.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> References: <37ACDF51E6FCB146B2D92527B733EB35027146A8@uksbirs-ukmg01.ukramsay.rhc.local> <1191565807.1794400.1285247834923.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24666@PHSXMB30.partners.org> I find it hard to believe as well. We have a refurbished unit and had to order a number of accessories (wash pots as well)within the past year. I will contact our sales rep and see what he says. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jsjurczak@comcast.net Sent: Thursday, September 23, 2010 9:17 AM To: Lesley Smith Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Leica XL autostainer wash pots Try Tech One or Marston, they are service companies and might have some. I can't believe Leica would discontinue parts for the XL. Are you sure this isn't just a temporary situation? That would be so unlike Leica to make things difficult for us..... ----- Original Message ----- From: "Lesley Smith" To: histonet@lists.utsouthwestern.edu Sent: Thursday, September 23, 2010 2:27:34 AM Subject: [Histonet] Leica XL autostainer wash pots We have just acquired a Leica XL Autostainer. However Leica have told us that the wash pots have been discontinued (and we need 5 of them!!) Does anyone have an unused XL gathering dust somewhere that we could recycle your wash pots! Fingers crossed and many thanks. ? Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW ? Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk ************************************************ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From AGleiberman <@t> cbiolabs.com Thu Sep 23 09:09:49 2010 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Thu Sep 23 09:09:55 2010 Subject: [Histonet] post-fixation question In-Reply-To: References: Message-ID: Hi Inan, 2h post-fixation for immunohistochemistry on mouse brain is usually more than enough. We use 1h post-fixation at room temp for mouse brain following storage (sometimes, for many weeks) at +4 C in PBS before sucrose cryo-protection, embedding and sectioning. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salim Yalcin Inan Sent: Wednesday, September 22, 2010 5:39 PM To: Histonet Subject: [Histonet] post-fixation question Hello, I have a question about post-fixation. As far as I know, for most of the immunohistochemistry studies in mouse/rat brain, post-fixation period is between 2 hours to 24 hours in 4% paraformaldehyde at +4 Celsius followed by cryoprotection with 20-30% sucrose solution. Are there any disadvantages to post-fix the tissues for more than 24 hours (2 days-1 week) for further immunohistochemistry experiments? Thank you very much in advance. Sincerely Yours, Salim Inan, PhD HBI, University of Calgary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From MW <@t> PersonifySearch.com Thu Sep 23 09:15:54 2010 From: MW <@t> PersonifySearch.com (Matthew Ward) Date: Thu Sep 23 09:16:01 2010 Subject: [Histonet] Outstanding Training Opportunity with a World Leader in Histology! Message-ID: <005f01cb5b29$d5be33f0$813a9bd0$@com> Good Morning, Our firm is currently partnered with a global leader in Histology that is going through a large expansion. We currently are searching for histology professionals who would be interested in working in house providing support and training to customers, clients, and current employees. Please contact me directly to learn more! Histology Training Specialist The Company: Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. The Opportunity: The company currently has an opening for a Histology Training Specialist. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: $55,000 - $80,000 Based on Experience Other: Full benefits - 401k program/matching Located in the Chicago area. Primary Responsibilities: The primary responsibility of this role will be to provide technical phone support by answering questions, troubleshooting problems, logging and closing complaint files and escalating major issues to appropriate company personnel. This role will also provide technical training on specified products in the company's newly constructed state-of-the-art Customer Support Laboratory. Training programs are designed for small groups to ensure maximum customer learning and satisfaction. Additional Responsibilities: - Provide product and applications phone support to end-users, field personnel and dealers for all product lines - Log all calls into Customer Support Database - Participate in development of training materials and conduct classes and labs for customers, employees and others as needed - Serve as technical liaison to Customer Service/Field Service/Product Management departments Education and Experience Required: Ability to interact with various people in a calm and positive fashion and the ability to effectively communicate information to groups of participants is required. Experience with data entry, MS Office programs (PowerPoint, Lotus Notes, Word) is also required. HT/HTL/QIHC (ASCP) is helpful but not required. Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From patrick.lewis <@t> seattlechildrens.org Thu Sep 23 10:05:33 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Thu Sep 23 10:05:49 2010 Subject: [Histonet] Sucrose cryoprotection Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C05BA3A2E@s107.childrens.sea.kids> Hi Histoneters Can someone explain sucrose cryoprotection to me. Why/when is it necessary? Tissue types that require it? Potential benefits/problems with its use or not use? Thanks. Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From SBarnes <@t> elch.org Thu Sep 23 10:11:56 2010 From: SBarnes <@t> elch.org (Sue Barnes) Date: Thu Sep 23 10:12:10 2010 Subject: [Histonet] Leica XL autostainer wash pots In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E24666@PHSXMB30.partners.org> Message-ID: <807C106E9A171C46B0CF253C2507971E15AD85@elchex01.elch.net> I just ordered and received wash vessels from Leica last week. The order number was 14045635268 The cost of each wash vessel was $165.00. I received them in about 8 days. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sherwood, Margaret Sent: Thursday, September 23, 2010 10:03 AM To: jsjurczak@comcast.net; Lesley Smith Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica XL autostainer wash pots I find it hard to believe as well. We have a refurbished unit and had to order a number of accessories (wash pots as well)within the past year. I will contact our sales rep and see what he says. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jsjurczak@comcast.net Sent: Thursday, September 23, 2010 9:17 AM To: Lesley Smith Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Leica XL autostainer wash pots Try Tech One or Marston, they are service companies and might have some. I can't believe Leica would discontinue parts for the XL. Are you sure this isn't just a temporary situation? That would be so unlike Leica to make things difficult for us..... ----- Original Message ----- From: "Lesley Smith" To: histonet@lists.utsouthwestern.edu Sent: Thursday, September 23, 2010 2:27:34 AM Subject: [Histonet] Leica XL autostainer wash pots We have just acquired a Leica XL Autostainer. However Leica have told us that the wash pots have been discontinued (and we need 5 of them!!) Does anyone have an unused XL gathering dust somewhere that we could recycle your wash pots! Fingers crossed and many thanks. ? Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW ? Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk ************************************************ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Sep 23 10:14:19 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Sep 23 10:14:24 2010 Subject: [Histonet] RELIA Special Job Alert 9/23/2010 Message-ID: Hi Histonetters!! I just had another 3 positions come in that I am pretty excited about. My clients offer excellent compensation, really nice benefits and great working environments. These are confirmed immediate full time permanent positions. While these are new clients for me, I have heard good things about them. If you are interested please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542 Here is the information on the positions: Histology Lab Manager - North Central Valley of CA Senior Immunohistochemistry Tech ? Southern Coastal GA Histotech with strong IHC experience ? Baton Rouge, LA I also have great opportunities in TX, NY, MA, TN and FL. If you know someone who might be interested please feel free to pass the information along to them as well. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From abijag76 <@t> rediffmail.com Thu Sep 23 11:42:42 2010 From: abijag76 <@t> rediffmail.com (abijag ) Date: Thu Sep 23 11:44:03 2010 Subject: [Histonet] rat kidney problems Message-ID: <20100923164242.47838.qmail@f6mail-145-190.rediffmail.com> Hello I need your advice regarding paraffin sections of rat kidney. Kidneys are collected, adequately fixed with 10 % NBF and undergone routine tissue processing in Sakura VIP 6 tissue processor.Sections are of 5 micron thickness. Strangely, in the H&E stained sections, our pathologists are complaining that typical morphology of tubules are not preserved ie. typical tubular patterns are missing (tubules with no lumen) in the cortical region (at the edges and slightly inwards). But the inner medulla and other regions are perfectly fine.This issue cropped up now only.Any body will give their expert advice to solve this issue. Thanks Abi From ctorrence <@t> kmcpa.com Thu Sep 23 12:05:49 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Thu Sep 23 12:06:04 2010 Subject: [Histonet] Out of Office Reply Message-ID: I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From schaundrawalton <@t> yahoo.com Thu Sep 23 12:45:00 2010 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Thu Sep 23 12:45:05 2010 Subject: [Histonet] Teaching Oportunity Message-ID: <771753.42567.qm@web120611.mail.ne1.yahoo.com> Keiser University is seeking a Program Director and a Clinical Coordinator for their AS in Histotechnology program at the Pembroke Pines campus.? Keiser University is a regionally accredited, private, career university that provides educational programs at the undergraduate and graduate levels for a diverse student body. The main campus is located in Fort Lauderdale with campuses located throughout the State of Florida and internationally. Through quality teaching, the University is committed to provide all students with opportunities to develop the knowledge, understanding, and skills necessary for successful employment. Committed to a students? first philosophy, Keiser University prepares graduates for careers in business, criminal justice, health care, technology, hospitality, education and career-focused general studies. Inherent in our Mission is service to the community. This service includes community partnerships, involvement with various constituencies and various continuing education programs. DESCRIPTION Program Directors are responsible for leveraging their expertise to develop, maintain and deliver education services to students through: Creating and Maintaining core curriculum across the institution Communicating and monitoring delivery of core curriculum Preparing course plans and material Delivering courses Monitoring progress/attendance Advising students Recording grades and submitting reports Histology Program Director must have a Bachelor's degree and 5 years of experience.? Must have HT or HTL (ASCP) and 2 years teaching/education experience. This is a full time position that requires day availability. The Pembroke Pines campus is also seeking a Histotechnology Clinical Coordinator. Keiser University offers competitive salaries and great benefits including medical, dental, vision, 401k, FSA, and much more. ? ? If interested please contact Tania Phillips-Associate Dean (954)431-4300 Schaundra Walton BS HTL(ASCP) University Department Chair-Histotechnology Keiser University 5600 Lake Underhill Rd. Orlando, FL 32807 From Dana.Spencer <@t> PCMH.COM Thu Sep 23 12:33:00 2010 From: Dana.Spencer <@t> PCMH.COM (Dana Spencer) Date: Thu Sep 23 13:04:40 2010 Subject: [Histonet] Job Opening Message-ID: <4C9B570C.536C.000A.0@PCMH.COM> Histotechnician II Pitt County Memorial Hospitalis an 861-bed, Level I Trauma Center, regional referral hospital and is the flagship hospital for University Health Systems of Eastern Carolina. We serve as the teaching hospital for the Brody School of Medicine at ECU. PCMH provides acute, intermediate, rehabilitation and outpatient services to more than 1.3 million people in 29 counties. The Histotechnician II should be competent in all areas of the Histology Lab including: embedding, cutting, special stains and immunohistochemistry. Qualified candidates must have a High School diploma, a minimum of two years? experience as a Histotechnician or related experience and be ASCP certified or eligible to certify. PittCounty Memorial Hospital offers all the benefits of Greenville, NC. The low cost of living is matched by a high quality of life in this progressive community in the sunny south, located only a short drive from Carolina?s magnificent seashore. For immediate consideration, please visit www.pcmhcareers.com to submit an online application or resume. For more information, call: 800-346-4307. We are diverse talents brought together by a common dedication: EOE. It all comes together@www.uhseast.com ------------------------------------------------------------------------------ The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. ============================================================================== From napoli <@t> siscom.net Thu Sep 23 13:07:49 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Thu Sep 23 13:07:54 2010 Subject: [Histonet] HM 500 M cryostat issue Message-ID: <4c9b9775.3ba.60d6.610384504@siscom.net> Hello all, I have recently been using an older model MICROM HM500 M cryostat and have been experiencing an annoying problem. When I cut a section I like to leave the top edge of the OCT anchored by not cutting through it all the way. This way I can pull tension on the bottom of the section with a paint brush and flatten it out before picking it up on the slide. The carriage that holds the chuck "drifts up" slightly when I cut the section, pulling the tissue and OCT up and away from the plate. I have never used a cryostat that had a carriage that drifted up in that position. Over the years, every cryostat I have used (including doing a lot of Mohs and other interoperative work)has enabled me to "stop" the handle at any position in which I need it to remain. Anyway, this made cutting some rather challenging sections of mouse kidney very difficult. I actually had someone stand next to me and hold the crank in place while I took the section (or else it would drift a bit). My sense is that the crank mechanism has something wrong that is causing it to not remain stationary once I take my hand off the wheel. Recently an equipment repair service came in and told me that the unit was designed that way and even that "if I talked to the Germans that made it, they would say it waas designed that way." (!!) wtf? At any rate, he took out the lead conterweight and said that he would have to modify the handle balance "to suit my needs" by cutting off some of the weight and proceeded to get out a hack saw and started trying to saw off some lead. He got nowhere with this and stated he would have to take the unit into the shop to do this. He was also kind of insulting in that he told me that what i wanted the thing to do wasnt what it was designed to do! Any thoughts? I have asked around a bit and other histology techs tell me that they see the "drifting" as an abnormal occurrence and that it shouldnt do it if it's working right. I think something is off with the coupling inside or with the calibration or balance of the wheel. Any one have a comment? First time in 16 years I have had a repair person tell me I am using the machine the wrong way. This repair service is not as experienced as the one I have used for many years and I think he knows what he is doing. Thanks From MariAnn.Mailhiot <@t> leica-microsystems.com Thu Sep 23 13:10:06 2010 From: MariAnn.Mailhiot <@t> leica-microsystems.com (MariAnn.Mailhiot@leica-microsystems.com) Date: Thu Sep 23 13:10:14 2010 Subject: [Histonet] Leica XL autostainer wash pots In-Reply-To: <37ACDF51E6FCB146B2D92527B733EB35027146A8@uksbirs-ukmg01.ukramsay.rhc.local> Message-ID: Hi Lesley I did check in our ordering system here at Leica and 14045635268 is still a viable number. I have copied a collegue of mine in Germany to help get the proper information to our Leica collegues in the UK. Stefanie will contact them with the information they need. You can use the part number I have provided to order the wash stations. Thanks Stepfanie for your assistance. Kindest Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Smith, Lesley" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Leica XL autostainer wash pots 09/23/2010 02:27 AM We have just acquired a Leica XL Autostainer. However Leica have told us that the wash pots have been discontinued (and we need 5 of them!!) Does anyone have an unused XL gathering dust somewhere that we could recycle your wash pots! Fingers crossed and many thanks. Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk ************************************************ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From napoli <@t> siscom.net Thu Sep 23 13:10:57 2010 From: napoli <@t> siscom.net (Andrew Burgeson) Date: Thu Sep 23 13:11:03 2010 Subject: [Histonet] Cryostat correction Message-ID: <4c9b9831.244.63f0.2014097781@siscom.net> lol...At the end of my last post I meant to say, "I DO NOT think he knows what he is doing!" oops From a.thotakura <@t> imperial.ac.uk Thu Sep 23 13:35:24 2010 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Thu Sep 23 13:35:52 2010 Subject: [Histonet] Necrotic and apoptotic cells Message-ID: Dear All, I want to do look for Necrotic and apoptotic cells on liver tumor sections. What staining and antibodies are good for this technique?. Please let me know. Thank you very much in advance. Many Thanks, Anil Kumar. From victor <@t> pathology.washington.edu Thu Sep 23 13:33:10 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Sep 23 13:36:10 2010 Subject: [Histonet] Fwd: [PPMA] See OmniTrax at UWMC Message-ID: <4C9B9D66.9000901@pathology.washington.edu> For any of you PowerPath users or anyone else curious about barcoding workflow, that are visiting Seattle, please see the message below. You may reply directly to Dr. Schmidt. Victor -------- Original Message -------- Subject: [PPMA] See OmniTrax at UWMC Date: Thu, 23 Sep 2010 10:31:29 -0700 (Pacific Daylight Time) From: Rodney Schmidt Reply-To: A mutual assistance forum for PowerPath users To: PowerPath Mutual Assistance There are several events in the next few weeks that may be bringing PowerPath users to Seattle. If any of you would like to visit the University of Washington Medical Center to see the best workflow-driving and comprehensive barcoding solution available anywhere (OmniTrax), please send me a private email. We?re anticipating being able to host tours on Monday, Tuesday and Wednesday during NSH and can work with your schedule if you?re in the area for other reasons (e.g. a users conference). Rodney A. Schmidt, M.D., Ph.D. Professor of Pathology Director of Medical Informatics University of Washington, Seattle, WA (206) 598-6462 (206) 344-0532 (pager) (206) 598-3803 (fax) ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -------------- next part -------------- _______________________________________________ PowerPath Mutual Assistance [PPMA] mailing list PPMA@u.washington.edu http://mailman2.u.washington.edu/mailman/listinfo/ppma From AGleiberman <@t> cbiolabs.com Thu Sep 23 13:44:27 2010 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Thu Sep 23 13:44:32 2010 Subject: [Histonet] Necrotic and apoptotic cells In-Reply-To: References: Message-ID: Anil, For apoptotic cells in the liver you can use In situ cell death detection kit, fluorescein (Roche, cat.#11 684 795 910) and use counterstain with different color (Cy3, AlexaFluor 546 or Texas Red) for the detection of endogenous IgG. Liver cells do not produce IgG, but necrotic cells are usually impregnated with plasma proteins - and bright staining for endogenous IgG is easy and reliable way to visualize such cells. Works well on FFPE sections after citrate boiling retrieval. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thotakura, Anil Kumar Sent: Thursday, September 23, 2010 2:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Necrotic and apoptotic cells Dear All, I want to do look for Necrotic and apoptotic cells on liver tumor sections. What staining and antibodies are good for this technique?. Please let me know. Thank you very much in advance. Many Thanks, Anil Kumar. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From macveigh <@t> usc.edu Thu Sep 23 15:02:39 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Thu Sep 23 15:02:28 2010 Subject: [Histonet] HM 500 M cryostat Message-ID: <3D36AE987F33438AB35038F412E5A4C9@DFS66DD1> Hi Andrew, I worked on different cryostats and I have memories of chuck going up. The section is cold and sturdy and I would just let it follow the chuck up as it is still attached to it. Then pull down, touch it to the knife so it sits there (it gets attached at the bottom) and pick it up. It is a bit annoying, but you get used to it. In your case, you will pay a fortune for service with no guarantee that the unit will work the way you want it. Who knows what lese will go wrong in the process... Michelle Aloni USC Keck School of Medicine From mcauliff <@t> umdnj.edu Thu Sep 23 15:48:36 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Sep 23 15:45:59 2010 Subject: [Histonet] rat kidney problems In-Reply-To: <20100923164242.47838.qmail@f6mail-145-190.rediffmail.com> References: <20100923164242.47838.qmail@f6mail-145-190.rediffmail.com> Message-ID: <4C9BBD24.30704@umdnj.edu> Greetings Abi: For cortical tubules (proximal convoluted tubules, PCT) to have a nice, open lumen one needs to fix by perfusion.This has been known for many years. Failing that, use a fix that works very rapidly in needed, alcoholic formalin for example, and slice the kidney into thin slices. What happens with less than optimal fixation is the brush border of the PCT collapses into the lumen, filling it. Regions that do not have a well developed brush border do not have this problem, as you see. Why you are seeing this "only now", I do not know. Geoff abijag wrote: > Hello > > I need your advice regarding paraffin sections of rat kidney. Kidneys are collected, adequately fixed with 10 % NBF and undergone routine tissue processing in Sakura VIP 6 tissue processor.Sections are of 5 micron thickness. Strangely, in the H&E stained sections, our pathologists are complaining that typical morphology of tubules are not preserved ie. typical tubular patterns are missing (tubules with no lumen) in the cortical region (at the edges and slightly inwards). But the inner medulla and other regions are perfectly fine.This issue cropped up now only.Any body will give their expert advice to solve this issue. > > > > Thanks > > > > Abi > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From AnthonyH <@t> chw.edu.au Thu Sep 23 17:52:54 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Sep 23 17:53:09 2010 Subject: [Histonet] post-fixation question In-Reply-To: <4C9B5D19.1000108@umdnj.edu> Message-ID: I agree with you Geoff Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Thursday, 23 September 2010 11:59 PM To: Salim Yalcin Inan Cc: Histonet Subject: Re: [Histonet] post-fixation question Merced is correct about longer fixation inducing more cross-linking, possibly making immunostaining more difficult. Much depends on the antigen you are looking for. I disagree that the tissue will become brittle, this is not my experience. Geoff Salim Yalcin Inan wrote: > Hello, > > > > I have a question about post-fixation. As far as I know, for most of > the immunohistochemistry studies in mouse/rat brain, post-fixation > period is between 2 hours to 24 hours in 4% paraformaldehyde at +4 > Celsius followed by cryoprotection with 20-30% sucrose solution. Are > there any disadvantages to post-fix the tissues for more than 24 hours > (2 days-1 week) for further immunohistochemistry experiments? Thank > you very much in advance. > > Sincerely Yours, > > Salim Inan, PhD > > HBI, University of Calgary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Tony_Reilly <@t> health.qld.gov.au Thu Sep 23 18:46:33 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Thu Sep 23 18:47:08 2010 Subject: [Histonet] HM 500 M cryostat issue In-Reply-To: <4c9b9775.3ba.60d6.610384504@siscom.net> References: <4c9b9775.3ba.60d6.610384504@siscom.net> Message-ID: <4C9C7378.471C.0039.0@health.qld.gov.au> Hi Andrew I have not used that model cryostat so this may not be the answer but I have experienced this on other cryostats when the microtome has been removed for cleaning and decontamination and reinstalled with the handle in the incorrect position resulting in the counterweight being totally out of whack. When the microtome is removed the handle will naturally fall to 6 o'clock however when it is reinstalled the handle needs to be at 12 o'clock for the balance to be correct. If this is not done correctly when the handle is released the chuck will always return to the up position. I hope this helps. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Andrew Burgeson" 24/09/2010 4:07 am >>> Hello all, I have recently been using an older model MICROM HM500 M cryostat and have been experiencing an annoying problem. When I cut a section I like to leave the top edge of the OCT anchored by not cutting through it all the way. This way I can pull tension on the bottom of the section with a paint brush and flatten it out before picking it up on the slide. The carriage that holds the chuck "drifts up" slightly when I cut the section, pulling the tissue and OCT up and away from the plate. I have never used a cryostat that had a carriage that drifted up in that position. Over the years, every cryostat I have used (including doing a lot of Mohs and other interoperative work)has enabled me to "stop" the handle at any position in which I need it to remain. Anyway, this made cutting some rather challenging sections of mouse kidney very difficult. I actually had someone stand next to me and hold the crank in place while I took the section (or else it would drift a bit). My sense is that the crank mechanism has something wrong that is causing it to not remain stationary once I take my hand off the wheel. Recently an equipment repair service came in and told me that the unit was designed that way and even that "if I talked to the Germans that made it, they would say it waas designed that way." (!!) wtf? At any rate, he took out the lead conterweight and said that he would have to modify the handle balance "to suit my needs" by cutting off some of the weight and proceeded to get out a hack saw and started trying to saw off some lead. He got nowhere with this and stated he would have to take the unit into the shop to do this. He was also kind of insulting in that he told me that what i wanted the thing to do wasnt what it was designed to do! Any thoughts? I have asked around a bit and other histology techs tell me that they see the "drifting" as an abnormal occurrence and that it shouldnt do it if it's working right. I think something is off with the coupling inside or with the calibration or balance of the wheel. Any one have a comment? First time in 16 years I have had a repair person tell me I am using the machine the wrong way. This repair service is not as experienced as the one I have used for many years and I think he knows what he is doing. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From ccrowder <@t> vetmed.lsu.edu Thu Sep 23 19:25:49 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Sep 23 19:26:16 2010 Subject: [Histonet] slide staining racks Message-ID: Hi - I am staining by hand for a research project and am in need of some 30 slide staining racks. If anyone has any that they want to get rid of, please contact me. I can use multiples so, even if you have only one. Cheryl From ctorrence <@t> kmcpa.com Fri Sep 24 12:09:37 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Fri Sep 24 12:09:49 2010 Subject: [Histonet] Out of Office Reply Message-ID: <112456ad568c476b9e2c8a127b81598d@51d4bc4741ae4f4799afeae489435228> I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From arany <@t> fas.harvard.edu Fri Sep 24 12:10:13 2010 From: arany <@t> fas.harvard.edu (Praveen Arany) Date: Fri Sep 24 12:10:11 2010 Subject: [Histonet] Plastic Sections Message-ID: <4C9CDB75.8000006@fas.harvard.edu> Hello, I was wondering if anyone knows of a commercial lab or academic institute that can help with plastic sections for mice/rat teeth? Thanks. Praveen Arany Grad Student, Harvard University, Cambridge MA. From lyork <@t> kwbpathology.com Fri Sep 24 12:24:08 2010 From: lyork <@t> kwbpathology.com (LYork) Date: Fri Sep 24 12:26:26 2010 Subject: [Histonet] Florida Histotech Message-ID: <40BF307CF78046D1A61EA7194E5BD069@KWBPA.local> KWB Pathology Associates in Tallahassee, Florida is currently seeking a full-time Histology Technician for a day shift position. Candidates must be HT or HTL (ASCP). Florida license (or eligibility) is required. We offer an excellent salary and benefits package. A drug screen and criminal background check are required. For more information contact Leigh York at 888-878-5143 ext. 7735 or lyork@kwbpathology.com. ************************************************************************************ This footnote confirms that this email message has been scanned by PineApp Mail-SeCure for the presence of malicious code, vandals & computer viruses. ************************************************************************************ From cls71877 <@t> sbcglobal.net Fri Sep 24 13:14:39 2010 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Fri Sep 24 13:14:45 2010 Subject: [Histonet] Per Diem Histotech Position in Reno Nevada Message-ID: <481900.42562.qm@web81203.mail.mud.yahoo.com> Hi all, We are currently seeking a per diem histotech for a small GI lab in Reno, Nevada.? If anyone is interested, please contact me. Thanks, Cristi From kblack <@t> digestivehlth.com Fri Sep 24 14:19:12 2010 From: kblack <@t> digestivehlth.com (Konni Black) Date: Fri Sep 24 14:19:25 2010 Subject: [Histonet] Part-time job opening Sumter, SC Message-ID: <26A53AFE9CEA4F4CA7D168A253C1BDE1@digestivehlth.com> Hello All, There is a part-time opening for an experienced HT or HTL in Sumter, SC which is located between Columbia and Florence, SC.. Create your own schedule, estimated 15 to 20 hours per week needed. Excellent pay, new space and equipment. Please contact Konni Black at 253-503-2560 or kblack@digestivehlth.com. Thank you kindly for your interest. From TGoins <@t> mt.gov Fri Sep 24 14:40:17 2010 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Sep 24 14:40:24 2010 Subject: [Histonet] West Nile IHC Message-ID: After many frustrating attempts, I would greatly appreciate any information on the successful application of IHC for the detection of West Nile Virus in fixed tissue. I have tried no retrieval, enzymatic and heat retrieval with no success on horse and bird tissues. Would someone please share successful protocols (retrieval) and antibody sources for equine and avian fixed tissues? Our tissues are formalin fixed and the detection method used is biotin-streptavidin, alkaline phosphatase with a new fuchsin chromogen (Vulcan Fast Red) Thank you in advance, Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana From sbaldwin <@t> mhhcc.org Fri Sep 24 15:13:04 2010 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Fri Sep 24 15:14:04 2010 Subject: [Histonet] GLASS SLIDES Message-ID: Histonetters I was wondering what histo labs do with their slides are they incinerated? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From amosbrooks <@t> gmail.com Fri Sep 24 18:38:42 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Sep 24 18:38:45 2010 Subject: [Histonet] Necrotic and apoptotic cells Message-ID: Hi, Tunel is commonly used to show both apoptosis & necrosis. Caspase 3 is good for Apoptosis. I just recently used a GR-1 antibody from AbCam that shows a lot of promise for necrosis. Amos Message: 7 Date: Thu, 23 Sep 2010 19:35:24 +0100 From: "Thotakura, Anil Kumar" Subject: [Histonet] Necrotic and apoptotic cells To: "Histonet@lists.utsouthwestern.edu" Message-ID: > Content-Type: text/plain; charset="iso-8859-1" Dear All, I want to do look for Necrotic and apoptotic cells on liver tumor sections. What staining and antibodies are good for this technique?. Please let me know. Thank you very much in advance. Many Thanks, Anil Kumar. From rjbuesa <@t> yahoo.com Sat Sep 25 09:56:02 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Sep 25 09:56:06 2010 Subject: [Histonet] GLASS SLIDES In-Reply-To: Message-ID: <99692.93335.qm@web65705.mail.ac4.yahoo.com> There are companies that dispose of them. Ren? J. --- On Fri, 9/24/10, Sara Baldwin/mhhcc.org wrote: From: Sara Baldwin/mhhcc.org Subject: [Histonet] GLASS SLIDES To: "Histonet" Date: Friday, September 24, 2010, 4:13 PM Histonetters I was wondering? what histo labs do with their slides are they incinerated? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216,? Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mimmopozzuoli <@t> gmail.com Sat Sep 25 10:04:30 2010 From: mimmopozzuoli <@t> gmail.com (Mimmo Pozzuoli) Date: Sat Sep 25 10:04:33 2010 Subject: [Histonet] FISH procedures Message-ID: A question for people working in urology and other types of histology labs... Do histotechnicians perform FISH procedures in your lab or is it required to have an MT on board to do such tests? I have heard of ISH being done in histology settings and imagine that the automation of them has lent a particular level of ease to the process. With more and more labs doing these types of procedures, what are the requirements for testing personnel? same as histology work under CLIA regs? From ctorrence <@t> kmcpa.com Sat Sep 25 12:05:16 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Sat Sep 25 12:05:32 2010 Subject: [Histonet] Out of Office Reply Message-ID: <71df7a364e484ae28450c7a90a2bb8cd@e9557774404f45edb0c841da3e56f1c6> I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From bstephen <@t> fastmail.fm Sat Sep 25 20:02:13 2010 From: bstephen <@t> fastmail.fm (Birgitta Stephenson) Date: Sat Sep 25 20:02:18 2010 Subject: [Histonet] Picrosirius Red and Plant material Message-ID: <1285462933.15148.1396889341@webmail.messagingengine.com> Hola Histonetters, I have been using Picrosirius Red stains on lifted archaeological reidues with great results. However I have a few questions. When I have mixed slides that have bone,muscle and plant material everything takes up the stain which I am presuming is normal and it is only the collagen fibers that change colour in cross polarised light.Is this correct that everything takes up the stain? On slides that have just seed products the slide residue does not fluoresce like the muscle tissue but it still glows slightly. I assume this is due to the properties of plant? However I have found that lignin when it takes up the dye turns yellow and displays pleochroism and birefringence (bright yellow)in cross polarised light. Has anyone ever used this dye for plant products or have any comments re these observations. Also some of the experimental slides with meat smears on them tend to glow bright red in cross polarised light but not yellow or orange...any thoughts? Again I am trying to differentiate plant and animal residues on the one slide preferably as easily as possible, however these residues are lifted from archaeological tools thousands of years old....very ancient CSI type work. Thanks Birgitta Stephenson, Archaeology Microscopy Research Lab University of Queensland -- Birgitta Stephenson bstephen@fastmail.fm -- http://www.fastmail.fm - Does exactly what it says on the tin From gu.lang <@t> gmx.at Sun Sep 26 04:03:30 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Sep 26 04:11:07 2010 Subject: AW: [Histonet] FISH procedures In-Reply-To: References: Message-ID: <51A3287C82844D1E805C29AD6236CE2F@dielangs.at> I work in a histolab in Austria as Biomedical Scientist. There's no differentiation between histotechs and MTs in this lab. We perform manual FISH on FFPET like Her2neu and others. The reading is done by pathologists. I think FISH belongs to high level duties in histolab and should be performed by well educated histo-people. This said, also FISH is a technique done step by step after a hopefully well-done protocol. The ability of troubleshooting is the point. Automatically performed FISH is a matter of instrument maintenance. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mimmo Pozzuoli Gesendet: Samstag, 25. September 2010 17:05 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH procedures A question for people working in urology and other types of histology labs... Do histotechnicians perform FISH procedures in your lab or is it required to have an MT on board to do such tests? I have heard of ISH being done in histology settings and imagine that the automation of them has lent a particular level of ease to the process. With more and more labs doing these types of procedures, what are the requirements for testing personnel? same as histology work under CLIA regs? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ctorrence <@t> kmcpa.com Sun Sep 26 12:07:20 2010 From: ctorrence <@t> kmcpa.com (ctorrence@kmcpa.com) Date: Sun Sep 26 12:07:37 2010 Subject: [Histonet] Out of Office Reply Message-ID: I will be out the office the week of September 20-24. I will return on Monday, September 27th. If you need laboratory assistance please call 785-273-2788 ext. 322. Thanks. From shive003 <@t> umn.edu Mon Sep 27 01:48:38 2010 From: shive003 <@t> umn.edu (shive003@umn.edu) Date: Mon Sep 27 01:48:42 2010 Subject: [Histonet] West Nile IHC In-Reply-To: References: Message-ID: Tresa, I've been staining for WNV for many years and we've had a number of papers published with our results. I'm currently at the NSH symnposium, but will forward our SOP to you when I return on Thursday. Just a quick note, though. Although we've had many positive results with birds (esp. raptors), I've only seen one positive cell in all the horses that I've tried staining. So the fact that you haven't had any positive equine tissues does not surprise me. It could be that the horse is an end-host of the virus, so there is no replication going on. The virus might be being degraded by cellular processes and thus, not recognized by the antibody. Jan Shivers Section Head IHC/Histo/EM Veterinary Diagnostic Laboratory University of Minnesota St. Paul, MN On Sep 24 2010, Goins, Tresa wrote: After many frustrating attempts, I would greatly appreciate any information on the successful application of IHC for the detection of West Nile Virus in fixed tissue. I have tried no retrieval, enzymatic and heat retrieval with no success on horse and bird tissues. > Would someone please share successful protocols (retrieval) and antibody sources for equine and avian fixed tissues? Our tissues are formalin fixed and the detection method used is biotin-streptavidin, alkaline phosphatase with a new fuchsin chromogen (Vulcan Fast Red) > >Thank you in advance, > >Tresa Goins >Veterinary Diagnostic Lab >Department of Livestock >Bozeman, Montana > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CThornton <@t> dahlchase.com Mon Sep 27 08:57:00 2010 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon Sep 27 08:57:06 2010 Subject: [Histonet] QA Questions Message-ID: I would love to hear some other labs' responses to these questions. For clarification, we are a private clinical lab, and we average about 50,000 cases/year. 1. What percentage of slides to you have that have greater than 1 fold? 2. On average what do your pathologists complain about as far as quality? 3. What is your Turn Around Time? What time do the pathologists start getting slides and what time do they have all of their work for the day? 4. What is your IHC turn around time? If the pathologists order IHC tests before what time do they have same day turn around time? Clare J.Thornton, HTL (ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From JWeems <@t> sjha.org Mon Sep 27 10:02:01 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Sep 27 10:02:09 2010 Subject: [Histonet] RE: QA Questions In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403986F35D7@CHEXCMS10.one.ads.che.org> 1. We don't count folds. 2. Quality of special stains and immunos. 3. First slides are ready by 8:00 and all out by 11:00. 4. IHCs ordered by noon are out same day. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Monday, September 27, 2010 09:57 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] QA Questions I would love to hear some other labs' responses to these questions. For clarification, we are a private clinical lab, and we average about 50,000 cases/year. 1. What percentage of slides to you have that have greater than 1 fold? 2. On average what do your pathologists complain about as far as quality? 3. What is your Turn Around Time? What time do the pathologists start getting slides and what time do they have all of their work for the day? 4. What is your IHC turn around time? If the pathologists order IHC tests before what time do they have same day turn around time? Clare J.Thornton, HTL (ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From kjones <@t> upei.ca Mon Sep 27 11:38:26 2010 From: kjones <@t> upei.ca (Kathleen Jones) Date: Mon Sep 27 11:38:46 2010 Subject: [Histonet] West Nile IHC Message-ID: <4CA09E410200008B0002D707@grpwise.novell.upei.ca> Hello Jan Could you please post this protocol on histonet, West Nile IHC testing is something out lab is definitely interested in pursuing. Much appreciated, and enjoy the S/C in Seattle! Kathy >>> 09/27/10 2:51 AM >>> Tresa, I've been staining for WNV for many years and we've had a number of papers published with our results. I'm currently at the NSH symnposium, but will forward our SOP to you when I return on Thursday. Just a quick note, though. Although we've had many positive results with birds (esp. raptors), I've only seen one positive cell in all the horses that I've tried staining. So the fact that you haven't had any positive equine tissues does not surprise me. It could be that the horse is an end-host of the virus, so there is no replication going on. The virus might be being degraded by cellular processes and thus, not recognized by the antibody. Jan Shivers Section Head IHC/Histo/EM Veterinary Diagnostic Laboratory University of Minnesota St. Paul, MN On Sep 24 2010, Goins, Tresa wrote: After many frustrating attempts, I would greatly appreciate any information on the successful application of IHC for the detection of West Nile Virus in fixed tissue. I have tried no retrieval, enzymatic and heat retrieval with no success on horse and bird tissues. > Would someone please share successful protocols (retrieval) and antibody sources for equine and avian fixed tissues? Our tissues are formalin fixed and the detection method used is biotin-streptavidin, alkaline phosphatase with a new fuchsin chromogen (Vulcan Fast Red) > >Thank you in advance, > >Tresa Goins >Veterinary Diagnostic Lab >Department of Livestock >Bozeman, Montana > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Fawn.Bomar <@t> HalifaxRegional.com Mon Sep 27 12:18:26 2010 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Mon Sep 27 12:22:16 2010 Subject: [Histonet] RE: QA Questions In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16403986F35D7@CHEXCMS10.one.ads.che.org> References: , <92AD9B20A6C38C4587A9FEBE3A30E16403986F35D7@CHEXCMS10.one.ads.che.org> Message-ID: 1. We don't count folds either. 2. Complains mainly about immunos 3. First slides come out about 8:30 and all are out by 10:30 4. If the IHC's are ordered by 11:30pm then we do same day turn around ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Monday, September 27, 2010 11:02 AM To: Clare Thornton; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: QA Questions 1. We don't count folds. 2. Quality of special stains and immunos. 3. First slides are ready by 8:00 and all out by 11:00. 4. IHCs ordered by noon are out same day. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Monday, September 27, 2010 09:57 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] QA Questions I would love to hear some other labs' responses to these questions. For clarification, we are a private clinical lab, and we average about 50,000 cases/year. 1. What percentage of slides to you have that have greater than 1 fold? 2. On average what do your pathologists complain about as far as quality? 3. What is your Turn Around Time? What time do the pathologists start getting slides and what time do they have all of their work for the day? 4. What is your IHC turn around time? If the pathologists order IHC tests before what time do they have same day turn around time? Clare J.Thornton, HTL (ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From Margaret.Perry <@t> sdstate.edu Mon Sep 27 14:32:48 2010 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Sep 27 14:32:53 2010 Subject: [Histonet] FW: WNV Message-ID: From: Perry, Margaret Sent: Monday, September 27, 2010 11:41 AM To: 'TGoins@mt.gov' Subject: WNV We have a problem with the equine tissue as do other labs. A lot of samples can be done and only one will come up positive. We have good luck with avian tissues. We use DAKO envision. The antibody we use is 7H2 (81-002) from BioReliance. Proteinase K for 6 min, antibody for 30 min, DAKO mouse envision for 30 min , nova red 4 min. Hope this helps. Our control tissue is avian heart and the cells that stain are very positive. However there are very few positive cells in our control and you really have to search to find them. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From collette2 <@t> llnl.gov Mon Sep 27 14:47:31 2010 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Mon Sep 27 14:47:43 2010 Subject: [Histonet] Blade sharpening service In-Reply-To: Message-ID: Hello, esteemed colleagues, I am in need of a service to have my 16cm tungsten carbide Profile D (a.k.a. "Sweeney Todd") blades resharpened. Can anyone recommend a service that is relatively accessible to the West coast? I looked in the archives and couldn't find any recent posts, and my Googling has been unproductive. I'd rather not send them back to Leica in Germany to have them sharpened if I don't have to. Thanks for all your help! Sincerely, Nicole Collette UC Berkeley/Lawrence Livermore National Lab From lentwistle <@t> ucsd.edu Mon Sep 27 15:20:46 2010 From: lentwistle <@t> ucsd.edu (Entwistle, Laura) Date: Mon Sep 27 15:23:23 2010 Subject: [Histonet] RE: Blade sharpening service In-Reply-To: References: , Message-ID: <0E51494EE610954592D9A04B07AAE35C13692A6A6B@MBX4.AD.UCSD.EDU> I believe McBain can sharpen them. They are located in the Los Angeles area. You can also try CIDCO. They are based out of San Diego. We have had our microtome blades sharpened by both places. Laura ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Collette, Nicole M. [collette2@llnl.gov] Sent: Monday, September 27, 2010 12:47 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Blade sharpening service Hello, esteemed colleagues, I am in need of a service to have my 16cm tungsten carbide Profile D (a.k.a. "Sweeney Todd") blades resharpened. Can anyone recommend a service that is relatively accessible to the West coast? I looked in the archives and couldn't find any recent posts, and my Googling has been unproductive. I'd rather not send them back to Leica in Germany to have them sharpened if I don't have to. Thanks for all your help! Sincerely, Nicole Collette UC Berkeley/Lawrence Livermore National Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From roosmith1 <@t> hotmail.com Mon Sep 27 18:01:56 2010 From: roosmith1 <@t> hotmail.com (Jackie Smith) Date: Mon Sep 27 18:02:00 2010 Subject: [Histonet] CAP Guideline: GEN.40942 Message-ID: Hello Histonetters, If anyone out in Histoland is willing to share a perspective regarding this CAP guidline question and its application in the histology lab I would greatly appreciate it. If there is a publication or reference available that would be equally appreciated! GEN.40942 Has the laboratory evaluated its specimen containers to ensure that they do not contribute to analytic interference in the assays to be performed? Kindest Regards, Eric Smith Celligent Diagnostics Charlotte, NC From louise.renton <@t> gmail.com Tue Sep 28 02:12:19 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Sep 28 02:12:44 2010 Subject: [Histonet] Blade sharpening service In-Reply-To: References: Message-ID: Delaware do ours ....and they're cheaper than leica On Mon, Sep 27, 2010 at 9:47 PM, Collette, Nicole M. wrote: > Hello, esteemed colleagues, > > I am in need of a service to have my 16cm tungsten carbide Profile D > (a.k.a. > "Sweeney Todd") blades resharpened. Can anyone recommend a service that is > relatively accessible to the West coast? I looked in the archives and > couldn't find any recent posts, and my Googling has been unproductive. I'd > rather not send them back to Leica in Germany to have them sharpened if I > don't have to. > > Thanks for all your help! > > Sincerely, > Nicole Collette > UC Berkeley/Lawrence Livermore National Lab > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From louise.renton <@t> gmail.com Tue Sep 28 02:12:19 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Sep 28 02:13:28 2010 Subject: [Histonet] Blade sharpening service In-Reply-To: References: Message-ID: Delaware do ours ....and they're cheaper than leica On Mon, Sep 27, 2010 at 9:47 PM, Collette, Nicole M. wrote: > Hello, esteemed colleagues, > > I am in need of a service to have my 16cm tungsten carbide Profile D > (a.k.a. > "Sweeney Todd") blades resharpened. Can anyone recommend a service that is > relatively accessible to the West coast? I looked in the archives and > couldn't find any recent posts, and my Googling has been unproductive. I'd > rather not send them back to Leica in Germany to have them sharpened if I > don't have to. > > Thanks for all your help! > > Sincerely, > Nicole Collette > UC Berkeley/Lawrence Livermore National Lab > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From tgenade <@t> gmail.com Tue Sep 28 02:43:25 2010 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Tue Sep 28 02:43:39 2010 Subject: [Histonet] gliotic plaques etc Message-ID: Hello, I'm interested in quantifying gliotic plaques, tangles and amyloid plaques in 5 um thick wax sections (fixed in Bouins). I'm planning on using Toluidine Blue for the amyloid and then Gallays Silver or Thioflavine S for the tangles. Any comments or suggestions? Any one have any experience with these stains for this purpose? The gliotic plaques are more confounding. I have seen a slide where H&E was used and the eosin stained the plaques bright pink-red. Is this typical? Otherwise, I was thinking about PTAH. Anyone have a favourite method for this purpose? I have about 400 slides to go through so an IHC method is out, being to expensive. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From louise.renton <@t> gmail.com Tue Sep 28 05:25:54 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Sep 28 05:25:59 2010 Subject: [Histonet] histomorphometry Message-ID: Hi all, I desperately need some advice from an experienced histomorphometrist....I am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From ratliffjack <@t> hotmail.com Tue Sep 28 08:36:29 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Sep 28 08:36:53 2010 Subject: [Histonet] histomorphometry In-Reply-To: References: Message-ID: At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanueva11@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathanael@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton wrote: > Hi all, > > I desperately need some advice from an experienced histomorphometrist....I > am trying to translate the old cumbersome visual measurement of using an > eypiece grid and doing point counting to an easier computer system - but I > am not sure how to do it - any help out there? > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nancy_schmitt <@t> pa-ucl.com Tue Sep 28 11:02:25 2010 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Sep 28 11:02:38 2010 Subject: [Histonet] RE: Histonet Digest, Vol 82, Issue 35 In-Reply-To: <20100927170316.77108158BE6@mail.pa-ucl.com> References: <20100927170316.77108158BE6@mail.pa-ucl.com> Message-ID: <737BD0BF52F0744B96B74B61756AC06443B280BA92@hestia.ad.pa-ucl.com> 1. We do not count folds. 2. Slide turnaround - but that is usually when we are short staffed - our pathology group does very little complaining - I know we are lucky! 3. First run of slide goes up at 730 and every 45 minutes after until all is out - we shoot for 1030. 4. Ordered by noon comes out the same day; after noon goes on the overnight run. Nancy Schmitt HT, MLT (ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext.142 Date: Mon, 27 Sep 2010 09:57:00 -0400 From: Clare Thornton Subject: [Histonet] QA Questions To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I would love to hear some other labs' responses to these questions. For clarification, we are a private clinical lab, and we average about 50,000 cases/year. 1. What percentage of slides to you have that have greater than 1 fold? 2. On average what do your pathologists complain about as far as quality? 3. What is your Turn Around Time? What time do the pathologists start getting slides and what time do they have all of their work for the day? 4. What is your IHC turn around time? If the pathologists order IHC tests before what time do they have same day turn around time? Clare J.Thornton, HTL (ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ------------------------------ Message: 4 Date: Mon, 27 Sep 2010 11:02:01 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: QA Questions To: Clare Thornton , "'histonet@lists.utsouthwestern.edu'" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403986F35D7@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" 1. We don't count folds. 2. Quality of special stains and immunos. 3. First slides are ready by 8:00 and all out by 11:00. 4. IHCs ordered by noon are out same day. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From sgoebel <@t> xbiotech.com Tue Sep 28 11:13:13 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Sep 28 11:13:17 2010 Subject: [Histonet] Tyramide Message-ID: <20100928091313.9e2d9aa830e8449a2412eb1e4f2f067e.ecd65be48a.wbe@email04.secureserver.net> Hello world...So trying to amplify my signal using tyramide. bought a kit from Invitrogen to use with fluorescence and the fluoresce said that h fluor. I called t never had my own questio guy was a moron on IHC, rude, and ya'll!! Any idea where I can b developer? My primary antibody is a just want to amplify with with the tyramide a DAB/hematoxylin. Any help would be awesome!!! Sarah Goebel, B.A., HT (ASCP) Histotechnician < 8201 East Riverside Austin, Texas& (512)386-5107 From anonwums1 <@t> gmail.com Tue Sep 28 11:21:19 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Tue Sep 28 11:21:23 2010 Subject: [Histonet] Tyramide In-Reply-To: <20100928091313.9e2d9aa830e8449a2412eb1e4f2f067e.ecd65be48a.wbe@email04.secureserver.net> References: <20100928091313.9e2d9aa830e8449a2412eb1e4f2f067e.ecd65be48a.wbe@email04.secureserver.net> Message-ID: I've had good luck with the biotinyl tyramide system from Perkin-Elmer Here is what I do using an unlabeled primary antibody 1) Block endogenous peroxide since you use peroxidase chemistry for this to work 2) Block avidin 3) Block biotin 4) Block with TNB buffer (the kit's equivalent of blocking serum) 5) Add primary antibody 6) Add biotinylated secondary antibody 7) Add SA-HRP (comes with kit) 8) Add biotinyl tyramide reagent. The HRP will deposit biotinylated tyramide near your antigen. 9) Add SA-HRP 10) Add DAB It works well. It also works if you replace step 9 with SA-fluorophore and don't use DAB at all. If your primary antibody is already labeled with HRP, you could do try this: 1) Block endogenous peroxide since you use peroxidase chemistry for this to work 2) Block avidin 3) Block biotin 4) Block with TNB buffer (the kit's equivalent of blocking serum) 5) Add primary HRP conjugated antibody 6) Add biotinyl tyramide reagent 7) Add SA-HRP 8) Add DAB I've never tried it, but I'd expect it won't amplify as well as my method. However, I see no reason why it shouldn't work. Good luck, Adam On Tue, Sep 28, 2010 at 11:13 AM, wrote: > > Hello world...So trying to amplify my signal using tyramide. ; I > bought a kit from Invitrogen to use with fluorescence and the > fluoresce nce didn't work at all! The PHD that is helping with this > said that h e thought we should try it using DAB instead of the > fluor. I called t he Invitrogen technical help...WOW!!! I have > never had my own questio n repeated back to me so many times, that > guy was a moron on IHC, rude, and should be fired!!! So I come to > ya'll!! Any idea where I can b uy tyramide to be used with a DAB > developer? My primary antibody is a lready conjugated with HRP, I > just want to amplify with with the tyramide a nd stain with > DAB/hematoxylin. Any help would be awesome!!! > > > Sarah Goebel, B.A., HT (ASCP) > > > > Histotechnician > > < i>X Biotech USA Inc. > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas& nbsp; 78744 > > (512)386-5107 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dchihc <@t> yahoo.com Tue Sep 28 14:22:28 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Sep 28 14:22:35 2010 Subject: [Histonet] HistoTech Position In Tuscaloosa Message-ID: <480630.811.qm@web43511.mail.sp1.yahoo.com> FINALLY another HistoTech position has been approved at DCH Regional Medical Center in Tuscaloosa, Alabama. We are a not for profit facility in West Alabama about 50 miles from Birmingham,?about 4 hours from the Gulf of Mexico, and about 3 hours from Atlanta. We process about 15000 surgicals per year using Sakura VIP conventional processor, and Sakura?ExPress 50 Rapid Tissue Processor, Sakura Prisma H&E Stainer with tape coverslipper and Ventana IHC automation. Hopefully we will be automated in Special Stains after October 1. Interested candidates must be proficient in embedding, microtomy, frozen sections, and (for now) manual special staining. Please contact Michelle Fagin at 205-759-7762 or Fax?205-750-5224 OR ????????????????????? Sherrie Faulkner at 205-750-5736 or email mfagin@dchsystem.com ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From Richard.Wagner <@t> BUEHLER.COM Tue Sep 28 15:47:55 2010 From: Richard.Wagner <@t> BUEHLER.COM (Wagner, Richard [BUL/LAK]) Date: Tue Sep 28 15:48:03 2010 Subject: FW: [Histonet] histomorphometry Message-ID: <1DCBB9383C918F46B98855D233367F670374C442@LAK01-EXCH.BUEHLER01.PRIV> Louise: Versatile image analysis software like OmniMet can solve this problem. Powerful digital image acquisition, object and field measurement options, and counting tools are standard features of the software. http://www.buehler.com/productinfo/biomedical/ia.htm Regards, Rick Wagner -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, September 28, 2010 8:36 AM To: louise renton Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histomorphometry At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanueva11@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathanael@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton wrote: > Hi all, > > I desperately need some advice from an experienced histomorphometrist....I > am trying to translate the old cumbersome visual measurement of using an > eypiece grid and doing point counting to an easier computer system - but I > am not sure how to do it - any help out there? > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ynwang <@t> u.washington.edu Tue Sep 28 17:14:50 2010 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Tue Sep 28 17:16:05 2010 Subject: [Histonet] histomorphometry In-Reply-To: <1DCBB9383C918F46B98855D233367F670374C442@LAK01-EXCH.BUEHLER01.PRIV> Message-ID: Hi Louise, If your lab is like other labs and are a bit strapped for money but need basic point counting, you may also be able do this using ImageJ. ImageJ is a free piece of software developed at and provided by NIH. It has grid and point overlays and counting tools. The learning curve isn't very steep, and it's free! http://rsbweb.nih.gov/ij/ There is also a pc version. Good luck Yak-Nam Wang On 9/28/10 1:47 PM, "Wagner, Richard [BUL/LAK]" wrote: > Louise: > > Versatile image analysis software like OmniMet can solve this problem. > Powerful digital image acquisition, object and field measurement > options, and counting tools are standard features of the software. > http://www.buehler.com/productinfo/biomedical/ia.htm > > Regards, > Rick Wagner > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack > Ratliff > Sent: Tuesday, September 28, 2010 8:36 AM > To: louise renton > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] histomorphometry > > At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva > demonstrated this very technique in his workshop. My best advice would > be to contact him directly (avillanueva11@cox.net) and see what he can > do for you. Another option would be to contact an image analysis vendor > like BIOQUANT (nathanael@bioquant.com). Hope this information helps! > > Regards, > > Jack > > On Sep 28, 2010, at 3:25 AM, louise renton > wrote: > >> Hi all, >> >> I desperately need some advice from an experienced > histomorphometrist....I >> am trying to translate the old cumbersome visual measurement of using > an >> eypiece grid and doing point counting to an easier computer system - > but I >> am not sure how to do it - any help out there? >> -- >> Louise Renton >> Bone Research Unit >> University of the Witwatersrand >> Johannesburg >> South Africa >> +27 11 717 2298 (tel & fax) >> 073 5574456 (emergencies only) >> "There are nights when the wolves are silent and only the moon howls". >> George Carlin >> No trees were killed in the sending of this message. >> However, many electrons were terribly inconvenienced. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From macveigh <@t> usc.edu Tue Sep 28 17:21:41 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Tue Sep 28 17:21:25 2010 Subject: [Histonet] Histomorphometry Message-ID: We use MetaMorph software. It comes with a digital camera for taking the pictures. You can get the software only if you have already the micrioscope and the camera. You can easily measure anything you want, but it is costly. Michelle USC School of Medicine LA CA From tliglesias <@t> ucdavis.edu Tue Sep 28 23:02:00 2010 From: tliglesias <@t> ucdavis.edu (Teresa Iglesias) Date: Tue Sep 28 23:02:04 2010 Subject: [Histonet] Vectastain elite ABC kit- multiple dips ok? Message-ID: Hi all, I have three 24-well plates with three brain sections in each well. For IHC, do I have to prepare three different/fresh ABC dips for each 24-well screen plate or can I incubate (30min) each successive screen plate into the already used dip of ABC? Do I have to leave it in longer to allow adequate binding? I know you can do this with DAB but you have a visual indicator that you've left it in long enough in that case. Any experience with this? Thanks, -Teresa -- ______________________________ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis ______________________________ From lksell <@t> aol.com Wed Sep 29 04:55:26 2010 From: lksell <@t> aol.com (lksell@aol.com) Date: Wed Sep 29 04:55:56 2010 Subject: [Histonet] (no subject) Message-ID: <8CD2DD3935C5761-1C94-8DD2@webmail-d035.sysops.aol.com> http://infioratadigerano.org/100298.html From mcauliff <@t> umdnj.edu Wed Sep 29 08:39:17 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Sep 29 08:36:29 2010 Subject: [Histonet] Vectastain elite ABC kit- multiple dips ok? In-Reply-To: References: Message-ID: <4CA34185.4080402@umdnj.edu> Teresa Iglesias wrote: > Hi all, > I have three 24-well plates with three brain sections in each well. For IHC, > do I have to prepare three different/fresh ABC dips for each 24-well screen > plate or can I incubate (30min) each successive screen plate into the > already used dip of ABC? I would not. > Do I have to leave it in longer to allow adequate > binding? > I may be that the ABC has been consumed by the first one or two 24 well plates? > I know you can do this with DAB but you have a visual indicator that you've > left it in long enough in that case. > Any experience with this? > Why not ask Vector? After all, they made the stuff. Geoff > Thanks, > -Teresa > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From cpyse <@t> x-celllab.com Wed Sep 29 09:12:57 2010 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Sep 29 09:14:51 2010 Subject: [Histonet] FW: DAB Message-ID: <000a01cb5fe0$6a6cf670$3f46e350$@com> From: Cynthia Pyse [mailto:cpyse@x-celllab.com] Sent: Tuesday, September 28, 2010 3:48 PM To: 'histonet-request@lists.utsouthwestern.edu' Subject: DAB Hello Histonetters I need some expert advice concerning the disposal of DAB. Our lab is in NYS and there has been some discussion on the proper way to dispose of DAB. I've done the research, and found household bleach is no longer acceptable. There is the potassium permanganate treatment, the horseradish peroxidase treatment, and also disposing of it the regulated medical waste that is incinerated. What is everyone using? Does NYS have different standards than other states or is this a federal regulation? If you dispose of the solution is the RMW, how do you protect the techs when pouring the waste DAB from our IHC waste container into bottles for disposal? If there is a spill can it be cleaned up within the lab or does a Hazmat team need to be called. I want to make sure we cover all our bases. I don't want any problems with NYS. Thank you in advance for all your input. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com From sfeher <@t> CMC-NH.ORG Wed Sep 29 10:49:35 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Sep 29 10:49:41 2010 Subject: [Histonet] Problems with listserv Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F5BD@exchange.cmc-nh.org> I am having some issues with receiving email from the listserv. I was successfully receiving email and it suddenly stopped. If I have been unsubscribed, please re subscribe my email address. sfeher@cmc-nh.org Thank you, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From marktarango <@t> gmail.com Wed Sep 29 11:05:53 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Sep 29 11:06:01 2010 Subject: [Histonet] Problems with listserv In-Reply-To: <73A7ED895EE0C24D9267ED814911DF191773F5BD@exchange.cmc-nh.org> References: <73A7ED895EE0C24D9267ED814911DF191773F5BD@exchange.cmc-nh.org> Message-ID: * *I think the chatter just died down since many of us are at the NSH symposium. Mark On Wed, Sep 29, 2010 at 8:49 AM, Feher, Stephen wrote: > I am having some issues with receiving email from the listserv. I was > successfully receiving email and it suddenly stopped. If I have been > unsubscribed, please re subscribe my email address. > > sfeher@cmc-nh.org > > Thank you, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histonet.nospam <@t> vneubert.com Wed Sep 29 11:06:35 2010 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Sep 29 11:06:40 2010 Subject: [Histonet] Problems with listserv In-Reply-To: <73A7ED895EE0C24D9267ED814911DF191773F5BD@exchange.cmc-nh.org> References: <73A7ED895EE0C24D9267ED814911DF191773F5BD@exchange.cmc-nh.org> Message-ID: <4CA3640B.7090209@vneubert.com> YMMD! You are the first I notice who wants to subscribe, not unscibe or unscribe or unsubscribe :D http://lists.utsouthwestern.edu/mailman/listinfo/histonet > I am having some issues with receiving email from the listserv. I was > successfully receiving email and it suddenly stopped. If I have been > unsubscribed, please re subscribe my email address. > > sfeher@cmc-nh.org > > Thank you, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Masterson_John <@t> Allergan.com Wed Sep 29 12:26:24 2010 From: Masterson_John <@t> Allergan.com (Masterson_John) Date: Wed Sep 29 12:28:24 2010 Subject: [Histonet] Bag Sealing system Message-ID: Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John

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From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Sep 29 12:47:17 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Sep 29 12:49:55 2010 Subject: [Histonet] RE: Bag Sealing system In-Reply-To: References: Message-ID: Milestone Medical has a really neat system. www.milesonemed.com It is called the TissueSAFE. You will find it under preanalytical tools. We would love to get one for our couriers and biospecimen repository. We haven't purchased it yet. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Masterson_John [Masterson_John@Allergan.com] Sent: Wednesday, September 29, 2010 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bag Sealing system Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John

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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Wed Sep 29 13:02:31 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Sep 29 13:02:36 2010 Subject: [Histonet] Bag Sealing system Message-ID: <20100929110231.9e2d9aa830e8449a2412eb1e4f2f067e.461feaca35.wbe@email04.secureserver.net> I have been using a food sealer from Walmart (or the likes) for y ears. You can buy the food seal bags in precut sizes or you can buy a the bags vary but are WAY less expansive then buying from a scientific su as time, if you budget saving Sarah Goebel, B.A., HT (ASCP) < Histotechnicia XBiotech USA Inc. < Austin, Texas 78744 < (512) -------- Original Message -------- Subject: [Histonet] Bag Sealing system From: Masterson_John <[1]Mas Date: Wed, September 29, 2010 10:26 am To: "[2]histonet@lists.ut <[3]histonet@lists.uts Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping John

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_______________________________________________ Histonet mailing list [4]Histonet@lists.utsouth [5]http: References 1. 3D"mailto:Masterson_John@Allergan.com" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:Histonet@lists.utsouthwestern.edu" 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From wdesalvo.cac <@t> hotmail.com Wed Sep 29 14:25:40 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Sep 29 14:25:45 2010 Subject: [Histonet] Bag Sealing system In-Reply-To: References: Message-ID: Cardinal sells the Kapac sealer and a variety of sizes of bags at 2 mm & 4 mm bags. This system is industrial and intended for laboratory use. We use to store tissue on-site and transport tissue and cassette between sites. has reduced storage space and incidence of fluid spills. William DeSalvo, B.S., HTL(ASCP) > From: Masterson_John@Allergan.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 29 Sep 2010 10:26:24 -0700 > Subject: [Histonet] Bag Sealing system > > Hello, > > Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. > > John > >

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> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdisbrow <@t> msn.com Wed Sep 29 14:26:10 2010 From: cdisbrow <@t> msn.com (Carrie Disbrow) Date: Wed Sep 29 14:26:14 2010 Subject: [Histonet] The Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Morphology Message-ID: HI! I was hoping to find out if I would be able to apply CEU's from the Feb 2011 Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Morphology to the State of Florida Department of Licensing. Does anyone know? There is not a lot of time to get the discounted rate for early registration and I would like to complete the process soon. Thanks for any info, Carrie Disbrow, BS, RVT, HTL (ASCP) From silvinamolinuevo <@t> yahoo.com.ar Wed Sep 29 14:36:47 2010 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Wed Sep 29 14:36:51 2010 Subject: [Histonet] histomorphometry Message-ID: <126836.10240.qm@web113620.mail.gq1.yahoo.com> Hi louise! like Yak-Nam WangI do use Image J. It is very easy to use, you can use just a picture and count particles/cells manually or do it? automated after defining the size threshold. The last way It will take you a bit more time until you validate the process, but it is no difficult. cheers, sil From Sharon.Davis-Devine <@t> carle.com Wed Sep 29 15:26:10 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Sep 29 15:26:16 2010 Subject: [Histonet] Used Microwave processor for sale Message-ID: We have a used Milestone RHS I Microwave Rapid Histoprocessor that we don't use anymore. We will be remodeling the histology lab in the near future and don't have room for it, so anyone out there in Histoland interested in this piece of equipment? It is in excellent shape and we have lots of extra parts for it. Contact me directly if interested. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From higginst <@t> amapath.com Wed Sep 29 16:05:54 2010 From: higginst <@t> amapath.com (Tim Higgins) Date: Wed Sep 29 16:10:20 2010 Subject: [Histonet] Ventana Ultraview DAB detection kits for sale. Message-ID: <000301cb601a$1c797850$6a03a8c0@apg> Hello Histonetters, We are overstocked on Ultraview DAB detection kits and looking to sale some for $2,300.00 per kit. We will pay for shipping within the continental US. The kits are NOT registered with the button still attached. We have 6 with the expiration Date: 4/2011 Lot #812955 We have 6 with the expiration Date: 5/2011 Lot # 812988 Please email me with your contact information if your interested. Thanks, Timothy Higgins, HT(ASCP) QIHC Histology Manager Amarillo Pathology Associates Amarillo, TX From alexia_fran <@t> hotmail.com Wed Sep 29 17:14:31 2010 From: alexia_fran <@t> hotmail.com (=?iso-8859-1?B?QWxleGlhIEZyYW5jaXNjYSBO+vFleiBQYXJyYQ==?=) Date: Wed Sep 29 17:14:36 2010 Subject: [Histonet] Free floating sections Message-ID: Hello Histonetters! I am currently running a immunofluorescence reaction using free floating brain sections and am having problems with the sections folding as I lift them to mount at the end of the reaction. To obtain the sections, I first perfused the mouse with 4%PFA, dissected the brain and postfixed it in 4%PFA for three hours, washed it twice in PBS solution, and stored the brain in PBS at 4 degrees celcius. Then, I used a vibratome to slice the brain in 100um sections (I have also tried using a cryostat - and tissue tek- but this immuno runs better on sections from the vibratome). After being sliced, the sections were stored at 4 degrees in PBS. I am running the immuno using a culture plate with incubation with a blocker (10%DS in PBS-T), primary antibody incubation overnight at RT, and incubation with secondary antibody at RT for two hours. All these incubations are made under agitation. Also, I am co-staining the cells with the nuclear stain TOPRO. To mount the sections, I am lifting them using a thin paintbrush and placing them into a petri dish filled with PBS. Then I drag the sections on top on a slide, apply Vectashield and the coverslip. Normally the sections should unfold as soon as they are placed in PBS solution, but many of them are staying folded on the brush. I have already tried incubating the primary antibody at 4C and I observed even more folding. Can you suggest anything to prevent this? Thank you very much Alexia Nunez-Parra Graduate Student University of Maryland From saby_joseph_a <@t> yahoo.com Wed Sep 29 18:37:51 2010 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Wed Sep 29 18:37:56 2010 Subject: [Histonet] Bag Sealing system In-Reply-To: References: Message-ID: <249753.21931.qm@web114420.mail.gq1.yahoo.com> Perhaps the best and least expensive tissue save bag sealer I have found can be purchased from ULINE.? There are benchtop models that easily fit in a hood, there are floor models.? The benchtop model we use cost less than $200 USD, easily fits in a hood, and has been totally trouble free for 2 years now. I hope this helps. Joe Saby, BA HT ________________________________ From: WILLIAM DESALVO To: masterson_john@allergan.com; histonet Sent: Wed, September 29, 2010 3:25:40 PM Subject: RE: [Histonet] Bag Sealing system Cardinal sells the Kapac sealer and a variety of sizes of bags at 2 mm & 4 mm bags. This system is industrial and intended for laboratory use. We use to store tissue on-site and transport tissue and cassette between sites. has reduced storage space and incidence of fluid spills. William DeSalvo, B.S., HTL(ASCP) > From: Masterson_John@Allergan.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 29 Sep 2010 10:26:24 -0700 > Subject: [Histonet] Bag Sealing system > > Hello, > > Can anyone recommend a bag and heat sealing system for archiving/shipping >tissue? Thanks in advance. > > John > >

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> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Wed Sep 29 18:41:28 2010 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Wed Sep 29 18:42:11 2010 Subject: [Histonet] RE: Bag Sealing system In-Reply-To: References: Message-ID: <4CA45B47.471C.0039.0@health.qld.gov.au> Hi Loralee Try a butcher's supply store. They sell devices they use for vacuum packing meat. By removing the air from the bag the evaporation of formalin is almost zero and the specimens will remain intact for many years. The bags are also more hardy than those sold with basic bag sealers. This is also a cheap way of preserving specimens for teaching purposes though depending on the shape of the specimen there can be some distortion due to the vacuum process. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "McMahon, Loralee A" 30/09/2010 3:47 am >>> Milestone Medical has a really neat system. www.milesonemed.com It is called the TissueSAFE. You will find it under preanalytical tools. We would love to get one for our couriers and biospecimen repository. We haven't purchased it yet. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Masterson_John [Masterson_John@Allergan.com] Sent: Wednesday, September 29, 2010 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bag Sealing system Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John

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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From smcbride <@t> andrew.cmu.edu Wed Sep 29 21:28:34 2010 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Wed Sep 29 21:28:38 2010 Subject: [Histonet] (no subject) Message-ID: Hello everyone, I have an old Leica RM 2065 microtome that is in need of repair (the clutch stopped working), but according to Leica, the machine is no longer supported by the company. Does anyone have any suggestions for a company or technician who might be able to repair the machine? Thanks in advance, ~Sean From gankam <@t> googlemail.com Wed Sep 29 21:55:02 2010 From: gankam <@t> googlemail.com (Fabrice GANKAM) Date: Wed Sep 29 21:55:13 2010 Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies In-Reply-To: References: Message-ID: Hey guys Just wanted to know if any of you had some luck with an antibody aigainst rat HMGB1 and its receptors RAGE, TLR2, TLR4. W Thanks Dr Fabrice GANKAM UTSW From histotech <@t> imagesbyhopper.com Thu Sep 30 05:39:32 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Sep 30 05:39:49 2010 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <3F3F35C1-5507-4680-BFE1-F2BF567D4D40@imagesbyhopper.com> Sean, what state are you in? If FL, there is a company called Southern Biomed who could probably help you out. Michelle On Sep 29, 2010, at 10:28 PM, "Sean McBride" wrote: > Hello everyone, > > I have an old Leica RM 2065 microtome that is in need of repair (the > clutch stopped working), but according to Leica, the machine is no longer > supported by the company. Does anyone have any suggestions for a company > or technician who might be able to repair the machine? > > Thanks in advance, > > ~Sean > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DKBoyd <@t> chs.net Thu Sep 30 07:46:00 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Sep 30 07:46:12 2010 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: Sean, Tech One @ 603-623-1271. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Sean McBride" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/29/2010 10:34 PM Please respond to smcbride@andrew.cmu.edu To histonet@lists.utsouthwestern.edu cc Subject [Histonet] (no subject) Hello everyone, I have an old Leica RM 2065 microtome that is in need of repair (the clutch stopped working), but according to Leica, the machine is no longer supported by the company. Does anyone have any suggestions for a company or technician who might be able to repair the machine? Thanks in advance, ~Sean _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From cstaak <@t> personifysearch.com Thu Sep 30 08:54:56 2010 From: cstaak <@t> personifysearch.com (Corey Staak) Date: Thu Sep 30 08:55:01 2010 Subject: [Histonet] Histology Professional needed in the Chicago Area!! Message-ID: <004a01cb60a7$10ec5600$32c50200$@com> World Leader in Cancer Diagnostics is looking for a Histology Trainer in Chicago. Great compensation ad Gold Standard Benefits!! Please contact: cstaak@personifysearch.com Corey Staak Executive Recruiter Laboratory Practice Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 112 (Fax) 919.460.8726 www.personifysearch.com Talent Management | Outsourced Recruitment From alyssa <@t> alliedsearchpartners.com Thu Sep 30 10:13:20 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Sep 30 10:13:33 2010 Subject: [Histonet] HT/HTL Job Opening in Florida Message-ID: Our client, located in Lakeland, FL has retained MPath Search Partners to assist in the search for a qualified Histotechnician/Histotechnologist in *Lakeland, FL*. This is a unique opportunity to join a financially strong and growing organization in a key role within the company. Shift: Full Time/Permanent, 3am-11am, Monday-Friday Environment: - Private Laboratory - Non-Stressed Environment & No Quotas - Team oriented and Friendly Laboratory Requirements: Florida Licensed Technician or Technologist ASCP certification Preferred Duties: - Routine Histology for Dermatology Clients - Grossing not required - IHC (willing to train if candidate is not experienced in IHC) Benefits: ? Three health plans to choose from - All through Blue Cross/Blue Shield ? 50% coverage for employee on Health and Dental Plan ? Long Term Disability ? 10 vacation/sick days (5 vacation days, 5 sick days the first year of employment) To apply: Please submit resume to alyssa@alliedsearchpartners.com . If all qualifications are met, we will schedule a phone interview between you and one of our recruiters. *Other Positions in Florida: Leesburg, FL, please ask for details if interested!* -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From rachel.paietta <@t> gmail.com Thu Sep 30 10:40:35 2010 From: rachel.paietta <@t> gmail.com (Rachel Paietta) Date: Thu Sep 30 10:40:40 2010 Subject: [Histonet] Bone Cracking During PMMA Embedding Message-ID: Hello, Our research group has been using a PMMA embedding protocol to embed bone samples and bone/soft tissue samples, but we have noticed recently in our BSE SEM images that we are getting an unacceptable amount of cracking. I am looking for some advice or any suggestions to prevent this and what other people have done if they have had a similar experience. We believe we are probably having some issues with the dehydration steps, but are unsure about what to change or what may be causing the issue. The procedure we use is as follows: Dehydration in Ethanol - 70% > 70% > 80% > 95% > 95% > 100%> 100% Acetone Monomer 1 (MMA, Benzoyl Peroxide and Dibutyl pthalate) Monomer 2 (MMA, Benzoyl Peroxid, Dibutyl Pthalate and PMMA beads) Monomer 3 (same as monomer 2 with more PMMA beads dissolved) Thanks in advance for your help. I am also posting a BSE image of one of our samples to illustrate the cracking. The file is:"PMMAembedded_sheep_spine_cracking.jpg" and is taken at 30x. Sincerely, Rachel -- Rachel Paietta PhD Candidate, Mechanical Engineering 427 UCB, University of Colorado at Boulder Boulder, CO 80301 paietta@colorado.edu From eridana <@t> cox.net Thu Sep 30 12:27:07 2010 From: eridana <@t> cox.net (Eridana) Date: Thu Sep 30 12:27:13 2010 Subject: [Histonet] Folding sections Message-ID: <20100930132708.XF1MD.357514.imail@fed1rmwml38> Hi Alexia I am not sure exactly what you are describing but my first question would be what is your nuclear stain in? If it is an alcoholic solution the sections may get shocked and stick together. I normally used DAPI in the secondary to counter-stain nuclei which is aqueous so that was less stress on the slices. I put 1-3ml of the 0.3% Triton in the mounting dish- it helped flatten and drag the slides on to the slides. For incubations I used micro centrifuge tubes with VERY slow rotation. Primary was ON at 4o. Rinses were in dishes from Brain Research. We changed the nets to panty hose and tightened them and glued them down with nail polish. I usually was staining 30u sliding microtome sections not vibrotome so may not cross over. I tried not to use paint brushes- they tended to damage the sections due to sticking to my sections. I made bent glass rods out of pasture pipettes with a bunsen burner. I once had the knife angle too steep on the sliding microtome, cut too fast and then had curled sections that never did flatten right. I do not know if that is is a possibility on the vibrotome. Good luck Donna Harclerode HT,HTL,QIHC (ASCP),SLS Histology Core Manager UCSD, Dept of Pathology 9500 Gillman Drive BSB 2009 San Diego, CA 92093 858 534 7438 Date: Wed, 29 Sep 2010 22:14:31 +0000 From: Alexia Francisca N??ez Parra Subject: [Histonet] Free floating sections To: lita de foro Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters! I am currently running a immunofluorescence reaction using free floating brain sections and am having problems with the sections folding as I lift them to mount at the end of the reaction. To obtain the sections, I first perfused the mouse with 4%PFA, dissected the brain and postfixed it in 4%PFA for three hours, washed it twice in PBS solution, and stored the brain in PBS at 4 degrees celcius. Then, I used a vibratome to slice the brain in 100um sections (I have also tried using a cryostat - and tissue tek- but this immuno runs better on sections from the vibratome). After being sliced, the sections were stored at 4 degrees in PBS. I am running the immuno using a culture plate with incubation with a blocker (10%DS in PBS-T), primary antibody incubation overnight at RT, and incubation with secondary antibody at RT for two hours. All these incubations are made under agitation. Also, I am co-staining the cells with the nuclear stain TOPRO. To mount the sections, I am lifting them using a thin paintbrush and placing them into a petri dish filled with PBS. Then I drag the sections on to a slide, apply Vectashield and the coverslip. Normally the sections should unfold as soon as they are placed in PBS solution, but many of them are staying folded on the brush. I have already tried incubating the primary antibody at 4C and I observed even more folding. Can you suggest anything to prevent this? Thank you very much Alexia Nunez-Parra Graduate Student University of Maryland From shive003 <@t> umn.edu Thu Sep 30 14:52:41 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Sep 30 14:52:45 2010 Subject: [Histonet] WNV IHC Message-ID: <6E02943B659445C881A08FF0882FB674@auxs.umn.edu> Hello everyone, A few people asked me to post some information on my West Nile Virus IHC test method, so here are some general comments. If you'd like more detailed information, please write to me directly. Tissue pretreatment - pressure cooker (Biocare Decloaking Chamber) with Target Retrieval solution (Dako) Primary antibody - anti-West Nile Virus (BioReliance, cat. # 81-002; clone 7H2; working dilution at 3-6 ug/ml Ab concentration, depending upon lot) Secondary linking antibody - EnVision+/HRP goat anti-mouse IgG (Dako; with 2-4% normal case species serum added per volume) Chromogen - AEC+ (Dako) Best regards, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.)