[Histonet] Coverslip removal question

Lewis, Patrick patrick.lewis <@t> seattlechildrens.org
Fri Oct 22 14:55:20 CDT 2010


Hi Histonetters

 

When I soak my slides in xylene to remove the coverslip, do I then need
to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and
then H20.

And then drying and re applying permount?

 

Or can I go straight from xylene to drying to re-coversliping with
permount?

 

 

 

Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

206-884-1115  OFFICE 

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206-884-7311  FAX

patrick.lewis <@t> seattlechildrens.org

OFFICE  1900 9th Avenue Seattle, WA 98101

MAIL      M/S C9S-8, Seattle, WA 98101

WWW     seattlechildrens.org <http://seattlechildrens.org/> 

 



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