[Histonet] phospho-Akt antibody

[Walters, Katherine S]

Dear Histo-netters,

I am trying to work up a phosphor-akt antibody (Cell Signaling #3787)
for my FFPE blocks of mouse uterine tubules.  Fixation is in Z-fix for
48 hours.  So far I have tried HIER with both microwave retrieval  and a
pressure-cooker.  I am using DAKO's En-vision plus rabbit secondary HRP
conjugate and reacting with DAB.  The results are less than impressive,
although I am seeing some low level staining at 1:50.  Has anyone used
this antibody with better success?

Thanks for any help in this matter.

Katherine Walters 
Histology Director 
Central Microscopy Research Facilities 
85 Eckstein Medical Research Building 
University of Iowa 
Iowa City, Iowa 52242-1101 
phone: # 319-335-8142 
fax:      # 319-384-4469 
katherine-walters <@t> uiowa.edu 
www.uiowa.edu/~cemrf