[Histonet] Optimal processing for prostate needle biopsies

[Rene J Buesa]

Nilesh:
1- if you are fixing with NBF do not think that because the biopsies are small require less time in the fixative. An "acceptable" fixing time will be 8 hours
2- the XpressX120 should give you good results IF the biopsies are well fixed.
3- Harris is a better hematoxylin because it is regressive and can be controlled better than Harris that is progressive and will need more staining time as it gets older/weaker
René J.

--- On Tue, 10/12/10, Gupta, Nilesh <NGUPTA1 <@t> hfhs.org> wrote:


From: Gupta, Nilesh <NGUPTA1 <@t> hfhs.org>
Subject: [Histonet] Optimal processing for prostate needle biopsies
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, October 12, 2010, 1:02 PM


I have a few questions regrading processing of prostate needle biopsies.
1.  What is optimal fixation time that the needle cores should be fixed for before loading these on the processors.
2.  Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides
3.  What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies.

Thanks

N.Gupta
Henry Ford Hospital

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