From louise.renton <@t> gmail.com Fri Oct 1 05:59:01 2010 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Oct 1 05:59:07 2010 Subject: [Histonet] histomorphometry - clarified Message-ID: Dear all, just to clarify issues here - i have a histomorphometry analysis system -a very nice one, and i sort of know how to use it - what i DON'T know is what sort of measurements i should be doing. So thanks to all who suggested systems, i'm sorry if my initial e-mail was not all that clear. further commentary on WHAT to measure and what parametres are useful will be welcomed best regards On Fri, Oct 1, 2010 at 12:12 PM, Katie McKinley wrote: > Dear Louise, > > We have software which not only will allow the easy measurement of slides > but also the management of your images. We specialize in whole slide > scanning software, We can scan your slides and your images are saved on our > network of servers and can be accessed online using secure login details. > Because the software is online you can access your images from any > location. > The scan can be treated like a glass slide. You can zoom in to diagnostic > resolution and pan around the whole slide and move through the layers. > As well as measurements, you can annotate the slide, build up archives and > banks of information and share with colleagues around the world instantly. > We are currently in beta testing of our off the shelf imaging analysis > software, but we do work on a consultancy basis to create analysis > algorithms to suit our clients' needs specifically. > > Please visit our website for more info. www.i-path.co.uk > > Kind Regards > > Katie > > -----Original Message----- > From: Wagner, Richard [BUL/LAK] [mailto:Richard.Wagner@BUEHLER.COM] > Sent: 28 September 2010 21:48 > To: louise.renton@gmail.com > Cc: histonet@lists.utsouthwestern.edu > Subject: FW: [Histonet] histomorphometry > > Louise: > > Versatile image analysis software like OmniMet can solve this problem. > Powerful digital image acquisition, object and field measurement > options, and counting tools are standard features of the software. > http://www.buehler.com/productinfo/biomedical/ia.htm > > Regards, > Rick Wagner > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack > Ratliff > Sent: Tuesday, September 28, 2010 8:36 AM > To: louise renton > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] histomorphometry > > At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva > demonstrated this very technique in his workshop. My best advice would > be to contact him directly (avillanueva11@cox.net) and see what he can > do for you. Another option would be to contact an image analysis vendor > like BIOQUANT (nathanael@bioquant.com). Hope this information helps! > > Regards, > > Jack > > On Sep 28, 2010, at 3:25 AM, louise renton > wrote: > > > Hi all, > > > > I desperately need some advice from an experienced > histomorphometrist....I > > am trying to translate the old cumbersome visual measurement of using > an > > eypiece grid and doing point counting to an easier computer system - > but I > > am not sure how to do it - any help out there? > > -- > > Louise Renton > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > +27 11 717 2298 (tel & fax) > > 073 5574456 (emergencies only) > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Rcartun <@t> harthosp.org Fri Oct 1 09:41:08 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Oct 1 09:41:22 2010 Subject: [Histonet] Marking inks Message-ID: <4CA5BAC3.7400.0077.1@harthosp.org> What are labs using for "marking inks" for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Rcartun <@t> harthosp.org Fri Oct 1 09:43:15 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Oct 1 09:43:24 2010 Subject: [Histonet] PCR for Yersinia Message-ID: <4CA5BB42.7400.0077.1@harthosp.org> Anyone doing PCR for Yersinia on formalin-fixed, paraffin-embedded tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From tgenade <@t> gmail.com Fri Oct 1 10:11:50 2010 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Oct 1 10:11:59 2010 Subject: [Histonet] gliotic plaques etc Message-ID: Hello, Not to be a nag, but I am still looking forward to some comments regarding the below: > Message: 8 > Date: Tue, 28 Sep 2010 09:43:25 +0200 > From: Tyrone Genade > Subject: [Histonet] gliotic plaques etc > I'm interested in quantifying gliotic plaques, tangles and amyloid > plaques in 5 um thick wax sections (fixed in Bouins). > > I'm planning on using Toluidine Blue for the amyloid and then Gallays > Silver or Thioflavine S for the tangles. Any comments or suggestions? > Any one have any experience with these stains for this purpose? > > The gliotic plaques are more confounding. I have seen a slide where > H&E was used and the eosin stained the plaques bright pink-red. Is > this typical? Otherwise, I was thinking about PTAH. Anyone have a > favourite method for this purpose? > > I have about 400 slides to go through so an IHC method is out, being > to expensive. I have come across the paper by Manlow et al (J. Neuropath. & Expr Neurology, 51:298-302, 1992) for a modified PTAH stain. Anyone used their method? Its not mentioned in "Theory and practice of histological techniques". Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 1 10:45:29 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Oct 1 10:45:46 2010 Subject: Recall: [Histonet] FW: DAB Message-ID: <1AAF670737F193429070841C6B2ADD4C02550101AF@EXMBMCB15.ucsfmedicalcenter.org> Morken, Tim would like to recall the message, "[Histonet] FW: DAB". From flnails <@t> texaschildrens.org Fri Oct 1 10:53:53 2010 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Oct 1 10:54:13 2010 Subject: [Histonet] Marking inks In-Reply-To: <4CA5BAC3.7400.0077.1@harthosp.org> References: <4CA5BAC3.7400.0077.1@harthosp.org> Message-ID: We use marking ink from Statlab or Mercedes Medical. After applying the ink to the tissue, we take weak acetic acid solution and blot it over the ink which helps it adhere. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 01, 2010 9:41 AM To: Histonet Subject: [Histonet] Marking inks What are labs using for "marking inks" for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From RHayden <@t> olmmed.org Fri Oct 1 10:57:29 2010 From: RHayden <@t> olmmed.org (Hayden, Rebecca) Date: Fri Oct 1 10:57:33 2010 Subject: [Histonet] Marking inks In-Reply-To: <4CA5BAC3.7400.0077.1@harthosp.org> References: <4CA5BAC3.7400.0077.1@harthosp.org> Message-ID: <7DDA4291CC9470439735E1E13FF4F58C89878288E3@EXCHMX.olmmed.org> I use inks from 3 different companies just because I need the variety of colors offered from each one. Fisher, Cardinal and a company in California. I have found that if I ink a specimen and dip the specimen in a solution of 10% acetic acid for a couple of seconds all ink shows up after processing wonderfully. I used to use straight acetone to dip in but found that you must leave the specimen for 10 seconds or longer for it to stay on after processing. One of these techniques might solve the problem. Good luck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 01, 2010 9:41 AM To: Histonet Subject: [Histonet] Marking inks What are labs using for "marking inks" for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice of Legal Status and Confidential Information: This electronic mail message and any accompanying attachments may contain information that is privileged and CONFIDENTIAL. If you are not the intended recipient you are advised that any use, review, dissemination, distribution or reproduction of the information is strictly prohibited and may be unlawful. If you have received this communication in error, please notify the sender immediately and destroy the transmitted information. From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 1 11:02:13 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Oct 1 11:02:28 2010 Subject: [Histonet] Marking inks In-Reply-To: <7DDA4291CC9470439735E1E13FF4F58C89878288E3@EXCHMX.olmmed.org> References: <4CA5BAC3.7400.0077.1@harthosp.org> <7DDA4291CC9470439735E1E13FF4F58C89878288E3@EXCHMX.olmmed.org> Message-ID: <1AAF670737F193429070841C6B2ADD4C02550101B1@EXMBMCB15.ucsfmedicalcenter.org> We use Davidson inks, except for the green. The green was gumming up our VIP processors. In fact we found out that Dr. Davidson does not recommend using the green for tissues to go through a processor, but that was not in the datasheet. So we use Cancer Research for the green ink. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayden, Rebecca Sent: Friday, October 01, 2010 8:57 AM To: 'Richard Cartun'; Histonet Subject: RE: [Histonet] Marking inks I use inks from 3 different companies just because I need the variety of colors offered from each one. Fisher, Cardinal and a company in California. I have found that if I ink a specimen and dip the specimen in a solution of 10% acetic acid for a couple of seconds all ink shows up after processing wonderfully. I used to use straight acetone to dip in but found that you must leave the specimen for 10 seconds or longer for it to stay on after processing. One of these techniques might solve the problem. Good luck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 01, 2010 9:41 AM To: Histonet Subject: [Histonet] Marking inks What are labs using for "marking inks" for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice of Legal Status and Confidential Information: This electronic mail message and any accompanying attachments may contain information that is privileged and CONFIDENTIAL. If you are not the intended recipient you are advised that any use, review, dissemination, distribution or reproduction of the information is strictly prohibited and may be unlawful. If you have received this communication in error, please notify the sender immediately and destroy the transmitted information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From higginst <@t> amapath.com Fri Oct 1 11:10:08 2010 From: higginst <@t> amapath.com (Tim Higgins) Date: Fri Oct 1 11:14:38 2010 Subject: [Histonet] Marking inks In-Reply-To: References: <4CA5BAC3.7400.0077.1@harthosp.org> Message-ID: <000101cb6183$1f929020$6a03a8c0@apg> We use Statlab and Fisher marking inks. We dip our specimens in Bouin's or Vinegar to set the ink. We don't have any problems with the ink coming off. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, October 01, 2010 10:54 AM To: 'Richard Cartun'; Histonet Subject: RE: [Histonet] Marking inks We use marking ink from Statlab or Mercedes Medical. After applying the ink to the tissue, we take weak acetic acid solution and blot it over the ink which helps it adhere. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 01, 2010 9:41 AM To: Histonet Subject: [Histonet] Marking inks What are labs using for "marking inks" for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Oct 1 11:16:11 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Oct 1 11:16:14 2010 Subject: [Histonet] Seeking accession stickers - rainbow 1x10 Message-ID: <436218.23369.qm@web50908.mail.re2.yahoo.com> Hello everyone!? Help!! ? We're about to run out of our usual accessioning label stickers.? ? These are single lines of 10 stickers on a pin-fed continuous form dot matrix sheet.? Each line of labels is one color rotating through 5 colors, so we have a numbering program that prints each line with one accession number, giving us 10 separate single-color stickers to put on our requisition, specimen bottle and cap.? ? The colors help us keep things from getting mixed up but our usual source is no longer available. ? Does anyone know of a source or an alternate way to produce labels to achieve the same end? ? Cheryl Kerry, HT(ASCP) From R.C.Stone <@t> comcast.net Fri Oct 1 11:40:38 2010 From: R.C.Stone <@t> comcast.net (R.C.Stone@comcast.net) Date: Fri Oct 1 11:40:27 2010 Subject: [Histonet] PA hourly wage Message-ID: <46F2261A344647CBA14E890E5A04F5C1@CynthiaPC> What is the average hourly wage for a PA that grosses approx. 5000 cases a year. __________ Information from ESET NOD32 Antivirus, version of virus signature database 5495 (20101001) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com From lbustamante <@t> cvm.tamu.edu Fri Oct 1 12:20:51 2010 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Fri Oct 1 12:21:22 2010 Subject: [Histonet] Correlation studies Message-ID: <4CA5D223020000B9000E04B9@CVM.TAMU.EDU> Posting for a friend. Please replay to me. If you have switched from Ventana Benchmark to Ventana Ultra. Could you please let us know what kind of correlation studies you did between machines.? Thank you very much. Susan Baker Saint Joseph Histology lab Supervisor Bryan, Texas. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 From Bill.Tench <@t> pph.org Fri Oct 1 12:26:35 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Oct 1 12:26:45 2010 Subject: [Histonet] change in immuno machines Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5647@MAIL1.pph.local> If you changed machines, you need to validate the staining on that machine just as if you never had the first machine. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From rsrichmond <@t> gmail.com Fri Oct 1 12:47:48 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Oct 1 12:47:56 2010 Subject: [Histonet] Re: Marking inks Message-ID: Richard Cartun asks about marking inks used for surgical pathology specimens. if I just need one color (great majority of specimens) I use india ink bought from a stationer or craft supply store - cheap, easily replaced, and it gets the job done. If I need colors, I prefer the Davidson marking inks, available through some though not all vendors. They offer seven colors, including orange (they have to sell their product in Tennessee and Texas, after all!) and purple. (I have no commercial connection with Davidson inks.) I blot my specimens thoroughly dry, and don't use a fixative. If I did, I'd use white vinegar (5% acetic acid), possibly diluted 1:1 with water. Bouin's fixative and acetone both have obvious environmental problems. Some people use tattoo inks. Cheap and available in a huge number of colors, but you have to read some truly disgusting catalogs to get them. I didn't know about green Davidson inks gumming up processors - thanks for that tip. (I wonder why other inks should be different.) With all marking inks, it's very important to re-cap the bottle as soon as you're through with it. If you re-cap promptly, an opened bottle will keep for years. Bob Richmond Samurai Pathologist Knoxville TN From alyssa <@t> alliedsearchpartners.com Fri Oct 1 13:34:14 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Oct 1 13:34:18 2010 Subject: [Histonet] Histotech Job Opening on Long Island Message-ID: Allied Search Partners has been retained to prescreen and search for a qualified Histotechnician or Histotechnologist candidate in New York, NY. Location: On Long Island, near Hicksville, NY Shift: - Full Time/Permanent (Long Term) - Day Shift 8am-5pm, Monday-Friday Environment: - A premier medical practice with several locations throughout NY and NJ consisting of about 24 doctors. - Private Laboratory and Team Oriented/Family Oriented Environment - No Quotas! Have the flexibility to get your work done on your own time and no weekends - State of the art equipment Requirements: - Ability to work with little or no supervision - NY licensed - ASCP certified - Meet the CLIA eligibility to gross Benefits & Pay: Salary is dependent upon experience and is competitive to current market rate Benefits can be discussed with Hiring Manager during a phone screen To apply: Please first submit resume to Alyssa@alliedsearchpartners.com . At that time we will review your resume with our team of recruiters and if all requirements are met will schedule a phone screen with you to discuss further questions and concerns. * * -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From higginst <@t> amapath.com Fri Oct 1 13:37:12 2010 From: higginst <@t> amapath.com (Tim Higgins) Date: Fri Oct 1 13:41:40 2010 Subject: [Histonet] change in immuno machines In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5647@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A5647@MAIL1.pph.local> Message-ID: <000001cb6197$aabbc720$6a03a8c0@apg> Agreed. Thanks, Timothy Higgins, HT(ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, October 01, 2010 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] change in immuno machines If you changed machines, you need to validate the staining on that machine just as if you never had the first machine. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BBranton <@t> sarapath.com Fri Oct 1 14:28:47 2010 From: BBranton <@t> sarapath.com (Brian Branton) Date: Fri Oct 1 14:28:35 2010 Subject: [Histonet] Dako Autostainer Plus for sale Message-ID: Hello Everyone, We have several pieces of equipment for sale, including our Dako Autostainer Plus (3800 Series) staining system. Please check us out at www.sarapath.com/equipment. Thanks, Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology Sarasota Professional Enterprises II (941) 362-8963 (941) 362-8964 FAX From rjbuesa <@t> yahoo.com Fri Oct 1 15:09:02 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 1 15:09:16 2010 Subject: [Histonet] PA hourly wage In-Reply-To: <46F2261A344647CBA14E890E5A04F5C1@CynthiaPC> Message-ID: <153314.62011.qm@web65702.mail.ac4.yahoo.com> Usually the hourly wage has nothing to do with the workload. That is something the PA has to negotiate with the lab manager. The work load will determine the number of PAs hired, if any. Ren? J. --- On Fri, 10/1/10, R.C.Stone@comcast.net wrote: From: R.C.Stone@comcast.net Subject: [Histonet] PA hourly wage To: histonet@lists.utsouthwestern.edu Date: Friday, October 1, 2010, 12:40 PM What is the average hourly wage for a PA that grosses approx. 5000 cases a year. __________ Information from ESET NOD32 Antivirus, version of virus signature database 5495 (20101001) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Oct 1 16:18:48 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Oct 1 16:19:01 2010 Subject: [Histonet] The Fifth Annual International Retreat on Applied IHC and Molecular Pathology References: <4CA6123C020000770001C587@gwmail4.harthosp.org> <4CA617F8020000770001C591@gwmail4.harthosp.org> Message-ID: <4CA617F7.7400.0077.0@harthosp.org> I am forwarding this announcement on behalf of Dr. Hadi Yaziji: Dear colleagues, We just finalized the schedule for our fifth annual course. Many of you have already attended our previous courses. Feel free to forward this email to any interested colleagues. Also, exhibitors are welcome for sponsorship opportunities. We have a limited room this year, based on first come-first serve basis. All information about schedule, location, time, faculty, exhibitors prospectus, contact info, registration form, are available and downloadable online from the course's website at www.pathlearning.com Thanks, Hadi ================================ Hadi Yaziji, MD I Medical Director Vitro Molecular Laboratories I www.vitromolecular.com 7480 SW 40th Street, Suite 700 Miami, FL 33155 Tel 305 267 7979 I Fax 786 513 0175 From amosbrooks <@t> gmail.com Fri Oct 1 21:44:44 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Oct 1 21:44:47 2010 Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies Message-ID: Hi, I am currently working on HMGB1. It is a weird one! If you go by the data sheets you will get great nuclear staining on all the nuclei. This is not what you are after if you have necrotic tissue. What you want to see is the nuclei in necrotic tissue fade out and have the cytoplasm take it up. I am cutting the dilution to 1: 50 and 1:100 since we were just starting to see blushes of cytoplasmic staining and no nuclear label at higher dilutions. Usually you get staining that fades out at higher dilutions. This one was really odd. Good luck, Amos Message: 13 Date: Thu, 30 Sep 2010 04:55:02 +0200 From: Fabrice GANKAM Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hey guys Just wanted to know if any of you had some luck with an antibody aigainst rat HMGB1 and its receptors RAGE, TLR2, TLR4. W Thanks Dr Fabrice GANKAM UTSW From gankam <@t> googlemail.com Mon Oct 4 08:21:15 2010 From: gankam <@t> googlemail.com (Fabrice GANKAM) Date: Mon Oct 4 08:21:22 2010 Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies In-Reply-To: References: Message-ID: thanks Amos which one did you used ? you have catalog number ? On Fri, Oct 1, 2010 at 9:44 PM, Amos Brooks wrote: > Hi, > I am currently working on HMGB1. It is a weird one! If you go by the > data sheets you will get great nuclear staining on all the nuclei. This is > not what you are after if you have necrotic tissue. What you want to see is > the nuclei in necrotic tissue fade out and have the cytoplasm take it up. I > am cutting the dilution to 1: 50 and 1:100 since we were just starting to > see blushes of cytoplasmic staining and no nuclear label at higher > dilutions. Usually you get staining that fades out at higher dilutions. This > one was really odd. > > Good luck, > Amos > > > Message: 13 > Date: Thu, 30 Sep 2010 04:55:02 +0200 > From: Fabrice GANKAM > Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > > Hey guys > Just wanted to know if any of you had some luck with an antibody aigainst > rat HMGB1 and its receptors RAGE, TLR2, TLR4. > W > Thanks > Dr Fabrice GANKAM > UTSW > From MadaryJ <@t> MedImmune.com Mon Oct 4 09:55:59 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Oct 4 09:56:08 2010 Subject: [Histonet] 3-6 month temp position in Gaithesburg Maryland Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A1302809397@MD1EV002.medimmune.com> `HT or HTL ASCP-tech will perform duties in a veterinary histology lab at Medimmune in Gaithersburg Md. Tech should be able to perform full-tissue necropsies on rodents, subsequent trimming, perform manual and automated tissue processing, decals, embedding in OCT and paraffin, microtomy and cryotomy, manual and automated HE staining and coverslipping. Should be proficient in manually performed special stains and immunohistochemistry. Should possess excellent troubleshooting skills, be familiar with GLP procedures and documentation. Tech should be clean and organized in the performance of his or her duties. The tech will be an Aerotek employee/contractor for Medimmune, not a Medimmune employee. This would be a straight up temp position, no benefits such as 401K, medical or dental. 20-40 hours per week. Perfect position for a person in between jobs, semi-retired etc. If you bring an exceptional set of skills with you but do not possess one of the skills listed above, we may work around that say you are excellent at necropsy but are not good at specials or IHC. Please submit resumes to Aerotek on your won to get the ball rolling, and then send a resume for the hiring manager Nick Madary at madaryj@medimmune.com. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From laurie.colbert <@t> huntingtonhospital.com Mon Oct 4 11:43:27 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Oct 4 11:43:31 2010 Subject: [Histonet] PI for Pathologists Message-ID: <57BE698966D5C54EAE8612E8941D768309A5A99F@EXCHANGE3.huntingtonhospital.com> Our pathologists do not like the CAP survey for their performance improvement program in Surgical Pathology. Does anyone know of any other company/organization that might offer something like this for our pathologists? From bamoe <@t> gundluth.org Mon Oct 4 12:49:44 2010 From: bamoe <@t> gundluth.org (bamoe@gundluth.org) Date: Mon Oct 4 12:51:02 2010 Subject: [Histonet] Direct immunofluoresent question Message-ID: Hi all - The pathologist that reads our direct IF slides likes to have the sections of tissue circled on the slide so that they are easier to find. We currently use black marker on the back of the slide, but find that many times it smears and are looking for a better solution. What kind of slides do others use, and if you circle your sections what marker/pen/etcher do you use? Any thoughts are greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI From arvidsonkristen <@t> yahoo.com Mon Oct 4 13:30:04 2010 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Mon Oct 4 13:30:16 2010 Subject: [Histonet] DIF Transport Media Message-ID: <5146.83227.qm@web65706.mail.ac4.yahoo.com> Is Michel Medium the same as Zeus?? Do these need to be refrigerated? Thanks. From rjbuesa <@t> yahoo.com Mon Oct 4 14:21:26 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 4 14:21:29 2010 Subject: [Histonet] Direct immunofluoresent question In-Reply-To: Message-ID: <55376.11510.qm@web65710.mail.ac4.yahoo.com> I used to mark on the slide, around the section, with a wax marker. Once dried and the procedure completed, cover and observe. Ren? J. --- On Mon, 10/4/10, bamoe@gundluth.org wrote: From: bamoe@gundluth.org Subject: [Histonet] Direct immunofluoresent question To: histonet@lists.utsouthwestern.edu Date: Monday, October 4, 2010, 1:49 PM Hi all - The pathologist that reads our direct IF slides likes to have the sections of tissue circled on the slide so that they are easier to find.? We currently use black marker on the back of the slide, but find that many times it smears and are looking for a better solution. What kind of slides do others use, and if you circle your sections what marker/pen/etcher do you use? Any thoughts are greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From neil.fournier <@t> yale.edu Mon Oct 4 14:37:59 2010 From: neil.fournier <@t> yale.edu (Neil M. Fournier) Date: Mon Oct 4 14:38:06 2010 Subject: [Histonet] IHC unfixed human brain tissue Message-ID: <20101004153759.uv6u4yb6oggkkckw@www.mail.yale.edu> I have limited experience working with human brain tissue and would like to stain some human brain sections that I received. They were not fixed, but were flash frozen and sectioned on a cryostat, and mounted onto slides. Could someone give me a brief run down of their standard methodological staining procedure with unfixed human brain sections? Much appreciated Neil From rjbuesa <@t> yahoo.com Mon Oct 4 14:44:50 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 4 14:44:55 2010 Subject: [Histonet] IHC unfixed human brain tissue In-Reply-To: <20101004153759.uv6u4yb6oggkkckw@www.mail.yale.edu> Message-ID: <248558.13040.qm@web65710.mail.ac4.yahoo.com> 1- they are very delicate, so treat them accordingly! 2- you do not need to use antigen retrieval, since they have been sectioned frozen. 3- go directly to block?Ab?detection?chromogen? H&E counterstain. 4- the times could be shorter than with FFPE sections. 5- wash very delicately. 6- dehydrate and cover as usual. Ren? J. --- On Mon, 10/4/10, Neil M. Fournier wrote: From: Neil M. Fournier Subject: [Histonet] IHC unfixed human brain tissue To: histonet@lists.utsouthwestern.edu Date: Monday, October 4, 2010, 3:37 PM I have limited experience working with human brain tissue and would like to stain some human brain sections that I received. They were not fixed, but were flash frozen and sectioned on a cryostat, and mounted onto slides. Could someone give me a brief run down of their standard methodological staining procedure with unfixed human brain sections? Much appreciated Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Mon Oct 4 14:50:20 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon Oct 4 14:50:26 2010 Subject: [Histonet] IHC unfixed human brain tissue In-Reply-To: <20101004153759.uv6u4yb6oggkkckw@www.mail.yale.edu> References: <20101004153759.uv6u4yb6oggkkckw@www.mail.yale.edu> Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D7302BC0@usctmx1176.merck.com> Neil, You need to do some fixation. Hopefully they are mounted on charged slides. We routinely post-fix fresh-frozen human brain sections in cold Acetone/ETOH (3:1) for 10 min and then proceed with the IHC staining. Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Neil M. Fournier Sent: Monday, October 04, 2010 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC unfixed human brain tissue I have limited experience working with human brain tissue and would like to stain some human brain sections that I received. They were not fixed, but were flash frozen and sectioned on a cryostat, and mounted onto slides. Could someone give me a brief run down of their standard methodological staining procedure with unfixed human brain sections? Much appreciated Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From matt <@t> techoneweb.com Mon Oct 4 16:40:42 2010 From: matt <@t> techoneweb.com (Matthew Mincer) Date: Mon Oct 4 16:40:29 2010 Subject: [Histonet] Histo job in Chicago Message-ID: <4CAA49DA.4040305@techoneweb.com> Hey All, I met with a guy today who mentioned he was looking for 3 or 4 techs for a histology lab he is opening. I told him I would post the opening on histonet. His information is: Dr. Nabil Hatoum Experimur Toxicology 4045 S Morgan St Chicago, IL 60609 773-254-2700 www.experimur.com Good luck Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell (708) 822-3738 From rsrichmond <@t> gmail.com Mon Oct 4 16:46:20 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Oct 4 16:46:24 2010 Subject: [Histonet] Re: PI for Pathologists Message-ID: From: "Laurie Colbert" Subject: [Histonet] PI for Pathologists Laurie Colbert at Huntington Hospital in Pasadena CA asks: >>Our pathologists do not like the CAP survey for their performance improvement program in Surgical Pathology. Does anyone know of any other company/organization that might offer something like this for our pathologists?<< Do your pathologists have any input into this decision? I think there are some local programs in California. You're probably referring to the "PIP" - the Performance Improvement Program in Surgical Pathology of the College of American Pathologists. I've subscribed since 1993 and have never thought very much of it. The CAP need to avail themselves of the new virtual slide technology (such as Aperio's) - this will become more attractive with the new DICOM image standards for pathology - and re-do the PIP program. Bob Richmond Samurai Pathologist Knoxville TN From AnthonyH <@t> chw.edu.au Mon Oct 4 17:57:36 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Oct 4 17:57:55 2010 Subject: [Histonet] Re: Marking inks In-Reply-To: Message-ID: The following might be of use Marking surgical margins are often required for determining the adequacy of excision of lesions. Sometimes up to 6 margins (medial, lateral, superior, inferior, superial and deep margins) are required to be individually marked. The properties of a good marker include: 1. It is quick to dry. 2. It is easy to apply. 3. It has a long shelf life. 4. It is cheap. 5. It is non-toxic. 6. It does not spread beyond the edge of the marked area. 7. It is preserved during processing (no contamination of solutions). 8. It is visible macroscopically and microscopically. 9. Sectioning is not affected. 10. It is radiolucent. 11. There are multiple colours available. India Ink, a colloidal suspension of particulate carbon in aqueous gum has been used for many years, but is messy, slow to dry and spreads readily over the tissue surface. 10% Silver Nitrate in methanol has been suggested but it also spreads over the surface and is macroscopically invisible. 5% alcian blue in 80% ethanol is an excellent tissue marker. It is easy to apply, quick to dry, has a long shelf life and is preserved during processing. Birch et al (1990) have suggested that specimens can be "dunked" into 1% aqueous alcian blue. This method is quick, cheap and the coating is radiolucent. Harris (1990) has suggested the use of Tippex fluid. This is a solution of titanium dioxide and polyacrylate in trichloethane. It has a long shelf life, is easy to use, quick drying, non-toxic and the tissue processor is not affected. The marker does however have a tendency to lift and is radiodense. Hunter-Craig et al (1991) rolled blotted dry specimens in starch powder. The surface coating of starch is visible on light microscopy but strikingly apparent if crossed polarisers are used. Artist's pigments in acetone (a 50% solution) were used by Patterson and Davies (1988). The pigments are quick drying, have a good range of different colours that are visible both macroscopically and microscopically (especially using polarised light) and are resistant to processing. They are however, radiodense and have a short storage life. It must be remembered that the dry powders (which contain cobalt, manganese and cadmium) are toxic. The pigment granules are also of similar size and density to microcalcification and there may be confusion on subsequent specimen mammography after tissue slicing. Suggested pigments are as follows: PIGMENT COLOUR MACROSCOPIC MICROSCOPIC Cobalt Blue Blue Blue Alizarin Crimson Red Red Viridian Green Green Armstrong et al (1990) have described the use of organically coloured gelatines, where 8% solution of dye is prepared in 24% aqueous gelatine. They found it easier to discriminate between colours of particulate dyes (i.e. plant substances), with the gelatine staining with both haematoxylin and eosin giving a pink to purple colour. They found clear demarcation between two adjacent colours and only a modest knowledge of botany was required for the identification of the three plant materials. DYE COLOUR MACROSCOPIC MICROSCOPIC Janus Green Blue Purple India Ink Black Black Paprika Red/brown Red-pigmented cellulose Tumeric Yellow Cerebriform starch granules Henna Brown Brown-pigmented cellulose Bismark Brown Brown Brown particles Unfortunately tumeric causes knife scratching. Armstrong et al (1990) J.Clin.Pathol., 43:604-607 Birch et al (1990) J.Clin.Pathol 43:608-609 Harris (1990) J.Clin.Pathol 43:346 Hunter-Craig et al (1991) J.Clin.Pathol. 44:874-875 Patterson & Davies (1988) J.Clin.Pathol 41:1013-1016 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Saturday, 2 October 2010 3:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Marking inks Richard Cartun asks about marking inks used for surgical pathology specimens. if I just need one color (great majority of specimens) I use india ink bought from a stationer or craft supply store - cheap, easily replaced, and it gets the job done. If I need colors, I prefer the Davidson marking inks, available through some though not all vendors. They offer seven colors, including orange (they have to sell their product in Tennessee and Texas, after all!) and purple. (I have no commercial connection with Davidson inks.) I blot my specimens thoroughly dry, and don't use a fixative. If I did, I'd use white vinegar (5% acetic acid), possibly diluted 1:1 with water. Bouin's fixative and acetone both have obvious environmental problems. Some people use tattoo inks. Cheap and available in a huge number of colors, but you have to read some truly disgusting catalogs to get them. I didn't know about green Davidson inks gumming up processors - thanks for that tip. (I wonder why other inks should be different.) With all marking inks, it's very important to re-cap the bottle as soon as you're through with it. If you re-cap promptly, an opened bottle will keep for years. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Bill.Tench <@t> pph.org Mon Oct 4 18:01:45 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Mon Oct 4 18:01:52 2010 Subject: [Histonet] CAP programs Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5663@MAIL1.pph.local> There are now online CAP PIP programs. I have done one on prostate. Also there are online programs for the cytology part. Review the catalogue. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From gagnone <@t> KGH.KARI.NET Tue Oct 5 09:53:36 2010 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Oct 5 09:53:46 2010 Subject: [Histonet] Direct immunofluorescence question Message-ID: Barb, Have you tried a diamond pencil? Available from a variety of sources, these "pencils" can be used to etch a circle or other shape around the tissue to be viewed. I believe they use lesser-quality industrial diamonds to make the tips on the pencils (i.e. not the diamond ring quality). Coming across the etched line under the fluorescent microscope produces brightness that helps the pathologist find the tissue. We circle our DIF's on the back of the slide - it won't wash off and won't interfere with reagents this way, but is still visible in a darkened room. Hope this helps, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From igor.deyneko <@t> gmail.com Tue Oct 5 12:07:21 2010 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Tue Oct 5 12:07:25 2010 Subject: [Histonet] Fibronectin AB for human tissues Message-ID: Dear Histonetters! I've been looking for a good Fibronecting antibody to stain some human tumors for connective tissues. I've tried 6 from Abcam, all either not working or giving high background. if anyone knows of a good antibody, i would really appreciate it. Thank you. Igor Deyneko Infinity Pharmaceuticals Molecular Pathology Cambridge, MA From pruegg <@t> ihctech.net Tue Oct 5 12:33:44 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Oct 5 12:34:52 2010 Subject: [Histonet] Rio-Hortega Silver Carbonate Stain Polak's variant? Message-ID: <76FEDDA5869348D48C9CB3A64C3AB1C8@Patsyoffice> Hi All, Question - Has anyone heard of a Rio-Hortega Silver Carbonate stain (Polak's variant) for mitochondria staining? Any suggestions? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From Ronald.Houston <@t> nationwidechildrens.org Tue Oct 5 12:45:21 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Oct 5 12:45:27 2010 Subject: [Histonet] Fibronectin AB for human tissues In-Reply-To: References: Message-ID: I use AbCam 32419 (rabbit monoclonal, clone F1) on the Bond at a dilution of 1:300 after EDTA retrieval; very clean, crisp results Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Tuesday, October 05, 2010 1:07 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Fibronectin AB for human tissues Dear Histonetters! I've been looking for a good Fibronecting antibody to stain some human tumors for connective tissues. I've tried 6 from Abcam, all either not working or giving high background. if anyone knows of a good antibody, i would really appreciate it. Thank you. Igor Deyneko Infinity Pharmaceuticals Molecular Pathology Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From srodriguez <@t> phenopath.com Tue Oct 5 12:56:09 2010 From: srodriguez <@t> phenopath.com (Stephanie Rodriguez) Date: Tue Oct 5 12:56:28 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 4 Message-ID: Barb, Eric, all- We also use a diamond pen/pencil-and they do contain an industrial grade diamond, so they are quite reasonably priced; I looked one up on the VWR website (just as a reference, not necessarily an endorsement! :o) and they run about $20-30 each. They last a very long time, too-We?ve had ours for several years. We use them for our direct IFs, FISH, anything dark field. Stephanie Stephanie Rodriguez, HTL(ASCP), QIHC Lead Molecular Technologist-FISH IHC Technologist III Phenopath Laboratories Seattle, WA (206) 374-9000 On 10/5/10 10:15 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > Message: 11 > Date: Tue, 5 Oct 2010 10:53:36 -0400 > From: "Gagnon, Eric" > Subject: [Histonet] Direct immunofluorescence question > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Barb, > > Have you tried a diamond pencil? Available from a variety of sources, these > "pencils" can be used to etch a circle or other shape around the tissue to be > viewed. I believe they use lesser-quality industrial diamonds to make the > tips on the pencils (i.e. not the diamond ring quality). Coming across the > etched line under the fluorescent microscope produces brightness that helps > the pathologist find the tissue. > > We circle our DIF's on the back of the slide - it won't wash off and won't > interfere with reagents this way, but is still visible in a darkened room. > > Hope this helps, > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada ____________________________________________________________________________ _________________________________________ Message: 1 Date: Mon, 4 Oct 2010 12:49:44 -0500 From: bamoe@gundluth.org Subject: [Histonet] Direct immunofluoresent question To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi all - The pathologist that reads our direct IF slides likes to have the sections of tissue circled on the slide so that they are easier to find. We currently use black marker on the back of the slide, but find that many times it smears and are looking for a better solution. What kind of slides do others use, and if you circle your sections what marker/pen/etcher do you use? Any thoughts are greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From smcbride <@t> andrew.cmu.edu Tue Oct 5 13:22:57 2010 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Tue Oct 5 13:23:02 2010 Subject: [Histonet] Rabbit Brain Tissue Processing Schedule Message-ID: Hello everyone, While I am well versed in protocols to process bone from a variety of species, my experience in processing soft tissue is less extensive. Currently, I do have some soft tissue protocols which I inherited from my predecessor, and with which I have generally had success, but I am know that there exists more tissue specific protocols than I am currently using. More specifically, I would like to find a protocol to fix, infiltrate and embed rabbit brains in paraffin. Would anyone have a protocol that they would not mind sharing? Thanks again to all of the folks on histonet who share their vast knowledge with those of us who are still learning. Best regards to all, ~Sean Sean McBride Senior Researcher Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-9807 (fax) smcbride@andrew.cmu.edu From dellav <@t> musc.edu Tue Oct 5 14:09:44 2010 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Oct 5 14:11:19 2010 Subject: [Histonet] DIF Transport Media In-Reply-To: <5146.83227.qm@web65706.mail.ac4.yahoo.com> References: <5146.83227.qm@web65706.mail.ac4.yahoo.com> Message-ID: Zeus was a company that used to market michel's under their own label. I don't believe zeus still exists. Michel's is the name associated with the author of the original paper. I don't have the reference. This solution does not require refrigeration. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Monday, October 04, 2010 2:30 PM To: histonet Subject: [Histonet] DIF Transport Media Is Michel Medium the same as Zeus?? Do these need to be refrigerated? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Oct 5 15:13:32 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Oct 5 15:13:53 2010 Subject: [Histonet] Re: Rio Hortega (Polaks) Message-ID: <000e01cb64c9$ca8f3ca0$5fadb5e0$@callis@bresnan.net> Patsy, Here is THE citation and abstract for this publication in J Histochem Cytochem (found it by Googling staining method). You can download the pdf at no charge. The photographs are excellent. Journal of Histochemistry and Cytochemistry Volume 52 (2): 211-216, 2004 Silver Carbonate Staining Reveals Mitochondrial Heterogeneity Jos? M. L?pez?Cepero Summary Silver staining methods, when selective, yield a high-contrast and high-resolution image in optical microscopy. A classical method for silver impregnation of mitochondria has been applied to murine tissues and reveals a marked heterogeneity among mitochondria in single cells. This heterogeneity can be detected in the optical microscope but is even more evident at the ultrastructural level. The differences in staining intensity may reflect different stages in the mitochondrial life cycle. The progressive accumulation of uranyl?argyrophilic material may be a marker of mitochondrial aging. This highly selective staining procedure may be of use in studies of mitochondrial changes under pathological conditions and during apoptosis. (J Histochem Cytochem 52:211?216, 2004) Key Words: mitochondria ? silver carbonate ? mitochondrial life cycle ? mitochondrial heterogeneity ? mitochondrial fusion Gayle M. Callis HTL/HT/MT(ASCP) From lbustamante <@t> cvm.tamu.edu Tue Oct 5 15:58:06 2010 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Tue Oct 5 15:58:39 2010 Subject: [Histonet] Osteoclast Message-ID: <4CAB4B0E020000B9000E0D52@CVM.TAMU.EDU> We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 From vjp2105 <@t> columbia.edu Tue Oct 5 16:04:16 2010 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Tue Oct 5 16:04:23 2010 Subject: [Histonet] Osteoclast In-Reply-To: <4CAB4B0E020000B9000E0D52@CVM.TAMU.EDU> Message-ID: You can do TRAP ( Tartrate-resistant Acidic Phosphatase) stain for osteoclasts. On 10/5/10 4:58 PM, "Lin Bustamante" wrote: We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Farnsworth <@t> cls.ab.ca Tue Oct 5 16:14:09 2010 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Tue Oct 5 16:14:14 2010 Subject: [Histonet] embedding beads/tissue marking dyes Message-ID: Hi We may be encountering some issues with our tissue marking dyes 'clogging' up our processors. I"ve heard of a system where a coloured 'bead' is embedded right beside the tissue, and subsequently cut onto the slide. This does not mark the margins obviously, but is used as a method to track like specimens that are grossed, embedded and subsequently cut in a row. (red, orange, green, blue.....) I explored the archives for "embedding beads", but only found reference to a glass bead that is placed in the wax inside the block, but not subsequently cut. PS: we are still troubleshooting our dyes (dilutions, brand, etc.), but if anyone has a brand of dye that they like and have no issues with, I'd be thrilled to get the information as well! Thanks in advance, Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From Maria.Katleba <@t> stjoe.org Tue Oct 5 16:40:26 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Oct 5 16:40:34 2010 Subject: [Histonet] RE: embedding beads/tissue marking dyes In-Reply-To: References: Message-ID: When you place dye on any tissue, make sure you pipette some bouins on to the tissue... Apparently the bouins "sets" the stain... afterwards dab the tissue with paper towel to sop up the excess dye/bouins. Some dyes are better than others, but try the bouins first Call me or email me directly, I can help you with details :) Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqueline Farnsworth Sent: Tuesday, October 05, 2010 2:14 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding beads/tissue marking dyes Hi We may be encountering some issues with our tissue marking dyes 'clogging' up our processors. I"ve heard of a system where a coloured 'bead' is embedded right beside the tissue, and subsequently cut onto the slide. This does not mark the margins obviously, but is used as a method to track like specimens that are grossed, embedded and subsequently cut in a row. (red, orange, green, blue.....) I explored the archives for "embedding beads", but only found reference to a glass bead that is placed in the wax inside the block, but not subsequently cut. PS: we are still troubleshooting our dyes (dilutions, brand, etc.), but if anyone has a brand of dye that they like and have no issues with, I'd be thrilled to get the information as well! Thanks in advance, Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From AnthonyH <@t> chw.edu.au Tue Oct 5 18:43:45 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Oct 5 18:43:56 2010 Subject: [Histonet] DIF Transport Media In-Reply-To: Message-ID: Vinnie, I hope you are well. The following might be of use: Specimens for immunofluorescence are usually submitted to the laboratory in cell culture fluid (eg Hanks or RPMI) or on saline soaked gauze. For transport to other institutions, Michel's Transport Medium has often been used: Michel's Buffer 1M potassium citrate, pH 7.0 2.5 ml 0.1M magnesium sulphate 5.0 ml 0.1M N ethylmalemide 5.0 ml Distilled H2O 87.5 ml * Mix well and store at 2 8oC. Exp. 1 year Michel's Transport Medium Michel's Buffer 100ml Ammonium sulphate 55gm Adjust pH to 7.0 7.4 with 1M KOH. Store at 2 8oC. Exp. 1 year Unfortunately Michel's Transport Medium has erroneously been called Michel's Fixative. None of the components of Michel's Transport Medium is a fixative. Ammonium sulphate precipitates antigen-antibody complexes in diseased skin and renal tissues. N ethylmalemide modifies free sulphydryl groups of cysteine residues in proteins (Fischer 2006). Michel's Transport Medium has been shown to be deleterious to morphology. Ultrastructurally, complete destruction of plasma membranes and intracytoplasmic organelles occurs after 48 hours storage. On the other hand, antigenicity is well preserved even after many days storage (Fischer 2006). Fischer (2006) Intern J Surg Pathol 14(1):108. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Wednesday, 6 October 2010 6:10 AM To: 'kristen arvidson'; histonet Subject: RE: [Histonet] DIF Transport Media Zeus was a company that used to market michel's under their own label. I don't believe zeus still exists. Michel's is the name associated with the author of the original paper. I don't have the reference. This solution does not require refrigeration. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Monday, October 04, 2010 2:30 PM To: histonet Subject: [Histonet] DIF Transport Media Is Michel Medium the same as Zeus?? Do these need to be refrigerated? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From szigcs <@t> bio.u-szeged.hu Wed Oct 6 02:33:47 2010 From: szigcs <@t> bio.u-szeged.hu (szigcs@bio.u-szeged.hu) Date: Wed Oct 6 02:33:58 2010 Subject: [Histonet] AChE fiber staining protocoll? Message-ID: <20101006093347.swij9v9us04088g4@webmail.u-szeged.hu> Dear Histonet Members! We are going to investigate AChE fiber density changes in the cortex of transcardially perfused rat brain slices (30 micrometer, criostat sections, perfusing solution: 4% formaldehyde in PB ph 7.4). We need a protocoll to visualize FINE fiber structure to be able to count changes. We used modification of Hedreen and original Tago protocoll. Hedreen good results for general, but no fiber staining, Tago no sucsses. Could you please tell us some advise, protocolls functioning on teh above mentioned objects? Thank you all in advance... Csaba Szigeti ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Oct 6 07:43:08 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Oct 6 07:44:12 2010 Subject: [Histonet] Osteoclast In-Reply-To: <4CAB4B0E020000B9000E0D52@CVM.TAMU.EDU> References: <4CAB4B0E020000B9000E0D52@CVM.TAMU.EDU> Message-ID: You can do a TRAP stain on EDTA decaled bone. I haven't been able to get it to work on formic acid decaled bone. I also believe that there is an antibody for osteoclasts, but I have not tried it. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante [lbustamante@cvm.tamu.edu] Sent: Tuesday, October 05, 2010 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Osteoclast We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kjgada <@t> gmail.com Wed Oct 6 08:15:58 2010 From: kjgada <@t> gmail.com (Komal Gada) Date: Wed Oct 6 08:16:03 2010 Subject: [Histonet] Question about Oil Red O controls Message-ID: Hello Histonetters, I am trying to find a procedure for using butter and egg yolks as controls for the Oil Red O stain (to show the fat). Does anyone have something they would be able to share with me? Thanks, Komal From arany <@t> fas.harvard.edu Wed Oct 6 08:17:15 2010 From: arany <@t> fas.harvard.edu (Praveen Arany) Date: Wed Oct 6 08:17:02 2010 Subject: [Histonet] Osteoclast In-Reply-To: References: <4CAB4B0E020000B9000E0D52@CVM.TAMU.EDU> Message-ID: <4CAC76DB.1070604@fas.harvard.edu> Hi Loralee, I am having similar problems with Formic acid Decal'd sections for TRAP. After EDTA, are you doing a TRAP IHC or the enzyme assay from Sigma? As for molecular markers, Cathepsin K is pretty good for Osteoclasts but I do westerns in a research setting......has anyone had good success with any antibody for immunostaining? Best, Praveen Arany, Graduate Student, Harvard University, Cambridge MA On 10/6/2010 8:43 AM, McMahon, Loralee A wrote: > You can do a TRAP stain on EDTA decaled bone. I haven't been able to get it to work on formic acid decaled bone. I also believe that there is an antibody for osteoclasts, but I have not tried it. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante [lbustamante@cvm.tamu.edu] > Sent: Tuesday, October 05, 2010 4:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Osteoclast > > We need to find a way to stain mainly Osteoclast. > Any suggestions? > Thank you very much. > Lin > > Lin S. Bustamante, B.Sc.; HT(ASCP) > Research Associate > Histology Lab Supervisor > Veterinary Integrative Bioscience > Texas A&M University > College Station, TX 77843-4458 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 41dmb41 <@t> gmail.com Wed Oct 6 08:22:52 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Wed Oct 6 08:23:18 2010 Subject: [Histonet] Question about Oil Red O controls In-Reply-To: References: Message-ID: Just use Mayo (not fat free, of course!) and smear it on the slide like you would a blood smear. It stains beautifully. Drew On Wed, Oct 6, 2010 at 09:15, Komal Gada wrote: > Hello Histonetters, > > I am trying to find a procedure for using butter and egg yolks as controls > for the Oil Red O stain (to show the fat). > > Does anyone have something they would be able to share with me? > > Thanks, > Komal > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Oct 6 08:25:17 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Oct 6 08:27:59 2010 Subject: [Histonet] Osteoclast In-Reply-To: <4CAC76DB.1070604@fas.harvard.edu> References: <4CAB4B0E020000B9000E0D52@CVM.TAMU.EDU> , <4CAC76DB.1070604@fas.harvard.edu> Message-ID: We were never able to get the TRAP working with Formic Acid decal. Using a home brewed TRAP kit or the Sigma Kit. But Cell Marque has a TRAcP antibody (catalog 341M-95) that is used as a hairy cell marker. But it also stains macrophages and osteoclasts. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Praveen Arany [arany@fas.harvard.edu] Sent: Wednesday, October 06, 2010 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Osteoclast Hi Loralee, I am having similar problems with Formic acid Decal'd sections for TRAP. After EDTA, are you doing a TRAP IHC or the enzyme assay from Sigma? As for molecular markers, Cathepsin K is pretty good for Osteoclasts but I do westerns in a research setting......has anyone had good success with any antibody for immunostaining? Best, Praveen Arany, Graduate Student, Harvard University, Cambridge MA On 10/6/2010 8:43 AM, McMahon, Loralee A wrote: > You can do a TRAP stain on EDTA decaled bone. I haven't been able to get it to work on formic acid decaled bone. I also believe that there is an antibody for osteoclasts, but I have not tried it. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante [lbustamante@cvm.tamu.edu] > Sent: Tuesday, October 05, 2010 4:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Osteoclast > > We need to find a way to stain mainly Osteoclast. > Any suggestions? > Thank you very much. > Lin > > Lin S. Bustamante, B.Sc.; HT(ASCP) > Research Associate > Histology Lab Supervisor > Veterinary Integrative Bioscience > Texas A&M University > College Station, TX 77843-4458 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Wed Oct 6 08:49:14 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Oct 6 08:49:25 2010 Subject: [Histonet] RE: embedding beads/tissue marking dyes Message-ID: <20101006064914.9e2d9aa830e8449a2412eb1e4f2f067e.03cfca699d.wbe@email04.secureserver.net> TBS has good dyes...you can mordant them (what Bouins does) in fo rmalin-aceto-alcohol. This is less toxic then Bouins and works well.& you don't wan is not known if it ef Happy Fixing!! PS-W =) Sarah Histotechnician < XBiot 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 [DEL: (512)386-5107 :DEL] -------- Original Message -------- Subject: [Histonet] RE: embedding beads/tissue marking dyes From: Maria Katleba <[1]Maria.Ka Date: Tue, October 05, 2010 2:40 pm To: Jacqueline Farnsworth <[2]Jacqueline.Farnsworth@cls.ab.ca>, "[3]Histonet@lists.utsout Histonet@lists.utsouthwestern.edu> When you place dye on any tissue, make sure you pipette some bouins on to t afterwards dab the t dye/bouins. Some dyes are better than others, but try the bouins first Call me or email me directly, I can help you with details :) Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: [5]histonet [[6]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqueline Farnsworth Sent: Tuesday, October 05, 2010 2:14 PM To: [7]Histonet@lists.uts Subject: [Histonet] embedding beads/tissue marking dyes Hi We may be encountering some issues with our tissue marking dyes 'clogging' coloured 'bead' is embed subsequently cut onto the slide. This doe obviously, but is used as a method to track like spe grossed, embedded and subsequently cut in a row. (red, oran green, blue.....) I explored the archives for "embedding beads", but only found reference to block, but not subsequent PS: we are still troubleshooting our dyes (dilutions, brand, etc.), but if with, I'd be th Thanks in advance, Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intende information. An disclosure is strictly in error, please notify the original message. Thank you. _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouth [9]http: Notice from St. Joseph Health System: Please note that the information contained in this message may be privilege _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouth [11]http: References 1. 3D"mailto:Maria.Katleba@stjoe.org" 2. 3D"mailto:Jacqueline.Farnsworth@cls.a 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"mailto:Histonet@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:histonet-bounces@l 7. 3D"mailto:Histonet@lists.utsouthwestern.edu" 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 10. 3D"mailto:Histonet@lists.utsouthwestern.edu" 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Ronald.Houston <@t> nationwidechildrens.org Wed Oct 6 08:50:07 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Oct 6 09:00:27 2010 Subject: [Histonet] AChE fiber staining protocoll? In-Reply-To: <20101006093347.swij9v9us04088g4@webmail.u-szeged.hu> References: <20101006093347.swij9v9us04088g4@webmail.u-szeged.hu> Message-ID: I have had great success in fine fibers with the modification of Martucciello et al, Eur J Pediatr Surg 2001; 11: 300-304, but only in 15 micron intestine sections, so I do not know how it will transpose to brain studies and the thicker sections Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of szigcs@bio.u-szeged.hu Sent: Wednesday, October 06, 2010 3:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AChE fiber staining protocoll? Dear Histonet Members! We are going to investigate AChE fiber density changes in the cortex of transcardially perfused rat brain slices (30 micrometer, criostat sections, perfusing solution: 4% formaldehyde in PB ph 7.4). We need a protocoll to visualize FINE fiber structure to be able to count changes. We used modification of Hedreen and original Tago protocoll. Hedreen good results for general, but no fiber staining, Tago no sucsses. Could you please tell us some advise, protocolls functioning on teh above mentioned objects? Thank you all in advance... Csaba Szigeti ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From SAllen <@t> exchange.hsc.mb.ca Wed Oct 6 09:02:54 2010 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Wed Oct 6 09:03:12 2010 Subject: [Histonet] FW: goat anti-rabbit FITC Message-ID: Hi, Could anyone please help us out? Does anyone use goat anti-rabbit FITC from Abcam? We would like to know the dilution used & any other hints you have. We will be using it on the Dako autostainer to detect rabbit C4d in renal frozen sections. I am sending this on behalf or our IHC lab, please respond to: dwatson@hsc.mb.ca or to me & I can pass it along. Thanks for any help you can provide. Sharon Allen HSC, Wpg, MB, Ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From KMB01 <@t> grh.org Wed Oct 6 09:19:53 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Wed Oct 6 09:19:59 2010 Subject: [Histonet] Helicobacter pylori Message-ID: Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From trathborne <@t> somerset-healthcare.com Wed Oct 6 09:25:37 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Oct 6 09:25:44 2010 Subject: [Histonet] Helicobacter pylori In-Reply-To: Message-ID: We use an Acridine Orange stain. You will need a fluorescent scope for it, though. It's a very simple procedure. Depariffinize slides, air dry, apply AO solution, let stand for 7 minutes, rinse, dry, coverslip from xylene. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kathy M. Gorham Sent: Wednesday, October 06, 2010 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter pylori Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From laurie.colbert <@t> huntingtonhospital.com Wed Oct 6 09:48:25 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Oct 6 09:48:32 2010 Subject: [Histonet] Helicobacter pylori In-Reply-To: Message-ID: <57BE698966D5C54EAE8612E8941D768309A5ACBC@EXCHANGE3.huntingtonhospital.com> We basically do a dif quik stain. I buy a kit called "Three Step Stain" from Cardinal. The total staining process takes about 1 minute. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, October 06, 2010 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter pylori Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From szigcs <@t> bio.u-szeged.hu Wed Oct 6 10:00:35 2010 From: szigcs <@t> bio.u-szeged.hu (szigcs@bio.u-szeged.hu) Date: Wed Oct 6 10:00:42 2010 Subject: [Histonet] AChE fiber staining protocoll? In-Reply-To: References: <20101006093347.swij9v9us04088g4@webmail.u-szeged.hu> Message-ID: <20101006170035.j2waztulso4480gg@webmail.u-szeged.hu> Id??zet ("Houston, Ronald" ): Thank you for the answer. We will try this protocol and i will reply the results. Csaba Szigeti > I have had great success in fine fibers with the modification of > Martucciello et al, Eur J Pediatr Surg 2001; 11: 300-304, but only > in 15 micron intestine sections, so I do not know how it will > transpose to brain studies and the thicker sections > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > szigcs@bio.u-szeged.hu > Sent: Wednesday, October 06, 2010 3:34 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AChE fiber staining protocoll? > > Dear Histonet Members! > > We are going to investigate AChE fiber density changes in the cortex > of transcardially perfused rat brain slices (30 micrometer, criostat > sections, perfusing solution: 4% formaldehyde in PB ph 7.4). We need a > protocoll to visualize FINE fiber structure to be able to count changes. > > We used modification of Hedreen and original Tago protocoll. Hedreen > good results for general, but no fiber staining, Tago no sucsses. > > Could you please tell us some advise, protocolls functioning on teh > above mentioned objects? > > Thank you all in advance... > Csaba Szigeti > > ---------------------------------------------------------------- > This message was sent using IMP, the Internet Messaging Program. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From JWeems <@t> sjha.org Wed Oct 6 10:01:52 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Oct 6 10:01:58 2010 Subject: [Histonet] RE: Helicobacter pylori In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640398777983@CHEXCMS10.one.ads.che.org> We use what we call a modified Genta. That is a modified Steiner if done manually. Because we have the Artisan and DAKO didn't have a Genta kit, I tried using the Warthin Starry and adding the Alcian blue and H&E manually and it worked fine. So we have not renamed it a modified Warthin Starry! But the H. pylori are very visible as well as the mucin and the morphology. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, October 06, 2010 10:20 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter pylori Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From dmccaig <@t> ckha.on.ca Wed Oct 6 10:03:05 2010 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Oct 6 10:03:16 2010 Subject: [Histonet] PAS STAIN Message-ID: Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana From JABiedermann <@t> uams.edu Wed Oct 6 10:04:55 2010 From: JABiedermann <@t> uams.edu (Biedermann, JoAnn) Date: Wed Oct 6 10:04:20 2010 Subject: [Histonet] Histokinette 2000 Message-ID: <833890C7E1BE584B926A2584E6B37ADC6B03F42E@MAIL2.ad.uams.edu> I have a Leica Histokinette 2000 that needs one beaker too be usable. Does anyone know where I can buy one of these? IMEB, inc does not have them. Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173 Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 JABiedermann@uams.edu Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From trathborne <@t> somerset-healthcare.com Wed Oct 6 10:06:12 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Oct 6 10:06:18 2010 Subject: [Histonet] PAS STAIN In-Reply-To: Message-ID: Were the new bottles from the same lot? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Wednesday, October 06, 2010 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From jclark <@t> pcnm.com Wed Oct 6 10:22:12 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Oct 6 10:22:19 2010 Subject: [Histonet] Query Special Stainers Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01375230@mail.pcnm.com> Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico From brandihiggins <@t> gmail.com Wed Oct 6 10:27:59 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Wed Oct 6 10:28:05 2010 Subject: [Histonet] Helicobacter pylori In-Reply-To: References: Message-ID: Hello, We use what is pretty much a diff quik stain, the stain is called QW and it is from poly scientific. The procedure is really easy. Deparaffinize, alcohols, methanol, water and then we do 15 slow dips in the QW2, 15 slow dips in the QW3, wash in water, 2 quick dips in alcohol and then dry in the oven, coverslip. Depending on your pathologist's preference you may need to adjust the dips in QW3 or the dips in the alcohol (which will decolorize slightly). Hope this helps! Brandi Higgins, BS, HT(ASCP) On Wed, Oct 6, 2010 at 10:19 AM, Kathy M. Gorham wrote: > Good Morning Histo land. I would like to know what others are using for > a special stain for Helicobacter pylori not IHC. Do you use a kit? From > where? Make up your own? Procedure. Thanks you. You have always been > so helpful. Kathy Gorham H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > Financial Excellence Award 2010 awarded by the national Rural Health > Research & Policy Analysis Center > Healthcare Achievement Award for Quality in Patient Care Delivery and > Satisfaction 2010 awarded by Amerinet > > GRH Mission > We will ensure access to high-quality, cost-effective health services in a > safe, customer-friendly environment for all those in need of our services. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s > only > and should be protected against viewing by unauthorized persons. The > information > herein may have been disclosed from records whose confidentiality is > protected > by Federal and State Law. Federal regulations prohibit further distribution > or > copying of this information without permission. If you received this > e-mail > transmission in error, please notify the sender immediately to arrange for > return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Wed Oct 6 10:39:03 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 6 10:39:10 2010 Subject: AW: [Histonet] PAS STAIN In-Reply-To: References: Message-ID: <986B2C6B449344EBAECEB4080A6E542D@dielangs.at> You can test the activity of the Schiffs. Add a few drops of formalin to a small amount of Schiffs. There should be the typical pink stain. The pH should be about 2. If there are white flakes in the bottle, this could be a sign for a too high pH. Is your periodic acid ok? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Diana McCaig Gesendet: Mittwoch, 06. Oktober 2010 17:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Oct 6 10:40:17 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Oct 6 10:40:38 2010 Subject: Where to buy Michels RE: [Histonet] DIF Transport Media In-Reply-To: References: Message-ID: <002801cb656c$c8bd8cd0$5a38a670$@callis@bresnan.net> Dear Tony, Thank you for the fine reply on Michel's Transport Media. We had excellent results with human renal biopsies destined for immunofluorescence staining. I don't recall ever exceeding 48 hours in Michels as 72 hours was allowed per recommendation from the original and Elias's publications. The kidney morphology didn't suffer excessively from this transport media as seen on our H&E stained frozen sections from Liquid Nitrogen cooled isopentane snap frozen needle biopsies. My renal pathologist always commented that the frozen section H&E looked very much like his the FFPE H&E section. This must vary from laboratory to laboratory and also what tissue was transported e.g. skin biopsies versus kidney biopsies. We always were very careful with good Michels buffer rinses. As for where to access Michels Transport Media and Michels buffer, we purchased these from Poly Scientific in much larger volumes at a cheaper price than Wampole(?)(Zeus). Aliquots were made and distributed to laboratories although that may not be ideal since busy laboratories may find this a bit labor intensive. I know of laboratories who make up Michels with great success. As for transporting tissue on saline soaked gauze, I can't stress the importance enough to NEVER let the tissue dry out, and snap freeze it as soon as it arrives in the laboratory. I would prefer receiving a tissue in cell culture fluid to ensure no drying. The reference is much appreciated. G'day and missed seeing you at NSH symposium/convention this year. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, October 05, 2010 5:44 PM To: Della Speranza, Vinnie; kristen arvidson; histonet Subject: RE: [Histonet] DIF Transport Media Vinnie, I hope you are well. The following might be of use: Specimens for immunofluorescence are usually submitted to the laboratory in cell culture fluid (eg Hanks or RPMI) or on saline soaked gauze. For transport to other institutions, Michel's Transport Medium has often been used: Michel's Buffer 1M potassium citrate, pH 7.0 2.5 ml 0.1M magnesium sulphate 5.0 ml 0.1M N ethylmalemide 5.0 ml Distilled H2O 87.5 ml * Mix well and store at 2 8oC. Exp. 1 year Michel's Transport Medium Michel's Buffer 100ml Ammonium sulphate 55gm Adjust pH to 7.0 7.4 with 1M KOH. Store at 2 8oC. Exp. 1 year Unfortunately Michel's Transport Medium has erroneously been called Michel's Fixative. None of the components of Michel's Transport Medium is a fixative. Ammonium sulphate precipitates antigen-antibody complexes in diseased skin and renal tissues. N ethylmalemide modifies free sulphydryl groups of cysteine residues in proteins (Fischer 2006). Michel's Transport Medium has been shown to be deleterious to morphology. Ultrastructurally, complete destruction of plasma membranes and intracytoplasmic organelles occurs after 48 hours storage. On the other hand, antigenicity is well preserved even after many days storage (Fischer 2006). Fischer (2006) Intern J Surg Pathol 14(1):108. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Wednesday, 6 October 2010 6:10 AM To: 'kristen arvidson'; histonet Subject: RE: [Histonet] DIF Transport Media Zeus was a company that used to market michel's under their own label. I don't believe zeus still exists. Michel's is the name associated with the author of the original paper. I don't have the reference. This solution does not require refrigeration. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Monday, October 04, 2010 2:30 PM To: histonet Subject: [Histonet] DIF Transport Media Is Michel Medium the same as Zeus?? Do these need to be refrigerated? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com From Janice.Mahoney <@t> alegent.org Wed Oct 6 10:49:22 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Oct 6 10:49:30 2010 Subject: [Histonet] RE: Query Special Stainers In-Reply-To: <0CDA5E1E01301F4880A8A7A8BCBDA39C01375230@mail.pcnm.com> References: <0CDA5E1E01301F4880A8A7A8BCBDA39C01375230@mail.pcnm.com> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F40@EXCHMBC2.ad.ah.local> We have 2 Ventana special stainers and they are very reliable work horses. They run every day over half the day and put out very consistent results. Very user friendly too. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, October 06, 2010 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Query Special Stainers Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From gayle.callis <@t> bresnan.net Wed Oct 6 11:04:44 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Oct 6 11:05:03 2010 Subject: [Histonet] PAS STAIN In-Reply-To: References: Message-ID: <003401cb6570$33037c00$990a7400$@callis@bresnan.net> I suspect bad Periodic acid. We never buy periodic acid already in solution and if that has been sitting around even in a kit, it may have gone bad. In a workshop taught by Charles Culling, an expert on PAS staining, he stressed making periodic acid fresh daily or each time the stain is done. It is not expensive, goes into solution readily, then toss the periodic acid to never be reused with exception of that one day. Also, you can test your Schiffs reagent by putting a few drops of Schiffs into 10 mls NBF, watch it turn a very bright pink red instantly. If it turns purplish, it is not good. It may be a bad kit, but you never said you were using a kit only "new bottles of ....." Consequently we buy periodic acid in crystalline form, make up 1% Periodic acid, and buy the Schiffs Reagent from Fisher, never a kit. Sigma also sells good Schiffs, but Fisher Scientific aka Thermo has larger quantity for less price. We also store our Schiffs in the refrigerator rather than room temperature, a habit left over from days when we made this reagent up in house. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, October 06, 2010 9:06 AM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PAS STAIN Were the new bottles from the same lot? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Wednesday, October 06, 2010 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com From FUNKM <@t> mercyhealth.com Wed Oct 6 11:08:04 2010 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Oct 6 11:08:15 2010 Subject: [Histonet] RE: Query Special Stainers In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F40@EXCHMBC2.ad.ah.local> References: <0CDA5E1E01301F4880A8A7A8BCBDA39C01375230@mail.pcnm.com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F40@EXCHMBC2.ad.ah.local> Message-ID: <4CAC5893.9B87.00AC.0@mercyhealth.com> We also have 2 Vantnan Special stainers and totally agree. Marcia M mason City Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> "Mahoney,Janice A" 10/06/2010 10:49 AM >>> We have 2 Ventana special stainers and they are very reliable work horses. They run every day over half the day and put out very consistent results. Very user friendly too. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, October 06, 2010 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Query Special Stainers Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jankeeping <@t> gmail.com Wed Oct 6 11:43:23 2010 From: jankeeping <@t> gmail.com (Janet Keeping) Date: Wed Oct 6 11:43:28 2010 Subject: [Histonet] PAS staining Message-ID: I have for some time had a problem with Schiff's reagent and PAS staining. - I have tested each new, unopened bottle of Schiff's reagent with formaldehyde and always the color development was immediate, but purple, definately not pink.This result has been quite consistant. - PAS staining for glycogen using the method in *Histotechnology a self Instructional text,* would fail to demonstrate any glycogen in autopsy liver specimens. I teach Histology at a community college and this problem has driven me crazy for a number of years. I have tried several brands of Schiff with the same results. Recently I obtained sheep tissues which were promptly refrigerated and fixed after death, and I had hoped these tissues would demonstrate glycogen. ( My thinking was that perhaps delay before autopsy was somehow diminishing the glycogen in the specimens that I had.or that perhaps long term NBF fixation had hampered staining.) Basement membranes were stained with the Schiff reagent as expected despite the purple color in the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced beautiful staining of fungus a lovely magenta color. A search of the web made me suspiciious when I noted that Schiff added to 37-40% formaldehyde should produce a pink or red color, however, A spot check of formalin using Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to ask if he could make any recommendation. Brian was not surprised by the purple color devopment in testing, He suggested that a large number of available aldehyde groups would be expected to produce this color. He suggested that I increase my periodate oxidation to 20-30 minutes and my Schiff application to 30 minutes. This worked and I am extremely grateful!. Has anyone else had an experience like this? Janet From Janice.Mahoney <@t> alegent.org Wed Oct 6 12:19:36 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Oct 6 12:23:37 2010 Subject: [Histonet] PAS staining In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F43@EXCHMBC2.ad.ah.local> Yes, I have experienced the same thing. Many people forget about the impact temperature has on some staining reactions. If you keep your Schiffs and Periodic acid in the refrigerator it may take longer to stain than it would if the reagents were at room temp. Many old procedures are written with the reagents at room temp (even the ones requiring refrigeration). This was one of those "live and learn" situations for me. Now temp and time are the first things I look at when a stain does not work on a known control. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: Wednesday, October 06, 2010 11:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS staining I have for some time had a problem with Schiff's reagent and PAS staining. - I have tested each new, unopened bottle of Schiff's reagent with formaldehyde and always the color development was immediate, but purple, definately not pink.This result has been quite consistant. - PAS staining for glycogen using the method in *Histotechnology a self Instructional text,* would fail to demonstrate any glycogen in autopsy liver specimens. I teach Histology at a community college and this problem has driven me crazy for a number of years. I have tried several brands of Schiff with the same results. Recently I obtained sheep tissues which were promptly refrigerated and fixed after death, and I had hoped these tissues would demonstrate glycogen. ( My thinking was that perhaps delay before autopsy was somehow diminishing the glycogen in the specimens that I had.or that perhaps long term NBF fixation had hampered staining.) Basement membranes were stained with the Schiff reagent as expected despite the purple color in the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced beautiful staining of fungus a lovely magenta color. A search of the web made me suspiciious when I noted that Schiff added to 37-40% formaldehyde should produce a pink or red color, however, A spot check of formalin using Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to ask if he could make any recommendation. Brian was not surprised by the purple color devopment in testing, He suggested that a large number of available aldehyde groups would be expected to produce this color. He suggested that I increase my periodate oxidation to 20-30 minutes and my Schiff application to 30 minutes. This worked and I am extremely grateful!. Has anyone else had an experience like this? Janet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Laura.Miller <@t> leica-microsystems.com Wed Oct 6 12:42:24 2010 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Wed Oct 6 12:42:32 2010 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 10/06/2010 and will not return until 10/07/2010. I am out sick today. I will respond to your message tomorrow. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From JanKeeping <@t> gmail.com Wed Oct 6 12:44:30 2010 From: JanKeeping <@t> gmail.com (JanKeeping@gmail.com) Date: Wed Oct 6 12:44:33 2010 Subject: [Histonet] PAS staining Message-ID: <001485e773625f4f520491f65410@google.com> Although I have allowed Schiff's and Periodic acid to room temperature, temperature may indeed be a factor. The ventilation system within this lab is extremely effficient and takes in a great deal of outside air. I am in Newfoundland, and lower outside temperatures are more common than we would like. :( Especially during the winter of course.) I just turned on the ventilation and the temperature dropped 2 degrees C in about five minutes. I will watch my room temperature more closely. Janet From gayle.callis <@t> bresnan.net Wed Oct 6 12:54:35 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Oct 6 12:54:55 2010 Subject: [Histonet] PAS staining In-Reply-To: References: Message-ID: <001a01cb657f$8b355010$a19ff030$@callis@bresnan.net> I was taught to avoid aqueous fixatives when trying to retain glycogen, soluble in NBF. Carnoys, Gendres fluid or acid alcohol formalin are three recommendations (Sheehan and Hrapchak Theory and Practice of Histotechnology) followed by starting processing in 95 to 100% alcohols. Alcoholic formalin should also work. It could very well be your long term storage in NBF has removed the glycogen, although basement membranes or fungus would not be affected. This was very apparent in a study done here where they wanted to see glycogen storage in mouse livers fixed in NBF for over a week and routinely processed starting in 70% alcohol. The glycogen, for all purposes, was removed even in experimental animals who had large quantity of glycogen in the cells (faintly stained but not what expected). Certainly increasing time in periodic acid and Schiffs can help. Also, one can increase the percentage of periodic acid from 0.5% to 1%, a hint Culling gave, as long as this is freshly made. However, we never use periodic acid for fungus staining, only chromic acid since periodic acid oxidation can give false negative results with Schiffs reagent. This is published in J Histotechnology by Carson and Fredenburgh. Interesting, but I still get a bright red pink color with Neutral buffered formalin test. I have never used concentrated 37% to 40% stock formaldehyde, only neutral buffered formalin (fixative) which would have fewer aldehyde groups available. Outdated, bad Schiffs always has the obvious purplish color with NBF. One thing we never allow is return used Schiffs back into stock Schiffs. Stock stain solutions are never contaminated with depleted, used solutions. We date when the Schiffs was used, and not reused within the week, this is discarded. This was particularly important with human renal biopsy work with the renal pathologist recommending disposing of used Schiffs. Our biopsy service did not handle a large number of biopsies in a year and this was a way to ensure consistent PAS staining by using only Schiffs. Successful PAS staining of basement membrane on 1 to 2 um zinc formalin or FFPE sections were never a problem. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: Wednesday, October 06, 2010 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS staining I have for some time had a problem with Schiff's reagent and PAS staining. - I have tested each new, unopened bottle of Schiff's reagent with formaldehyde and always the color development was immediate, but purple, definately not pink.This result has been quite consistant. - PAS staining for glycogen using the method in *Histotechnology a self Instructional text,* would fail to demonstrate any glycogen in autopsy liver specimens. I teach Histology at a community college and this problem has driven me crazy for a number of years. I have tried several brands of Schiff with the same results. Recently I obtained sheep tissues which were promptly refrigerated and fixed after death, and I had hoped these tissues would demonstrate glycogen. ( My thinking was that perhaps delay before autopsy was somehow diminishing the glycogen in the specimens that I had.or that perhaps long term NBF fixation had hampered staining.) Basement membranes were stained with the Schiff reagent as expected despite the purple color in the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced beautiful staining of fungus a lovely magenta color. A search of the web made me suspiciious when I noted that Schiff added to 37-40% formaldehyde should produce a pink or red color, however, A spot check of formalin using Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to ask if he could make any recommendation. Brian was not surprised by the purple color devopment in testing, He suggested that a large number of available aldehyde groups would be expected to produce this color. He suggested that I increase my periodate oxidation to 20-30 minutes and my Schiff application to 30 minutes. This worked and I am extremely grateful!. Has anyone else had an experience like this? Janet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com From gayle.callis <@t> bresnan.net Wed Oct 6 12:58:12 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Oct 6 12:58:31 2010 Subject: [Histonet] PAS staining In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F43@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F43@EXCHMBC2.ad.ah.local> Message-ID: <001b01cb6580$0c99b3d0$25cd1b70$@callis@bresnan.net> Jan is correct, and unless specified in a staining protocol, room temperature is used. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, October 06, 2010 11:20 AM To: 'Janet Keeping'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PAS staining Yes, I have experienced the same thing. Many people forget about the impact temperature has on some staining reactions. If you keep your Schiffs and Periodic acid in the refrigerator it may take longer to stain than it would if the reagents were at room temp. Many old procedures are written with the reagents at room temp (even the ones requiring refrigeration). This was one of those "live and learn" situations for me. Now temp and time are the first things I look at when a stain does not work on a known control. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: Wednesday, October 06, 2010 11:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS staining I have for some time had a problem with Schiff's reagent and PAS staining. - I have tested each new, unopened bottle of Schiff's reagent with formaldehyde and always the color development was immediate, but purple, definately not pink.This result has been quite consistant. - PAS staining for glycogen using the method in *Histotechnology a self Instructional text,* would fail to demonstrate any glycogen in autopsy liver specimens. I teach Histology at a community college and this problem has driven me crazy for a number of years. I have tried several brands of Schiff with the same results. Recently I obtained sheep tissues which were promptly refrigerated and fixed after death, and I had hoped these tissues would demonstrate glycogen. ( My thinking was that perhaps delay before autopsy was somehow diminishing the glycogen in the specimens that I had.or that perhaps long term NBF fixation had hampered staining.) Basement membranes were stained with the Schiff reagent as expected despite the purple color in the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced beautiful staining of fungus a lovely magenta color. A search of the web made me suspiciious when I noted that Schiff added to 37-40% formaldehyde should produce a pink or red color, however, A spot check of formalin using Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to ask if he could make any recommendation. Brian was not surprised by the purple color devopment in testing, He suggested that a large number of available aldehyde groups would be expected to produce this color. He suggested that I increase my periodate oxidation to 20-30 minutes and my Schiff application to 30 minutes. This worked and I am extremely grateful!. Has anyone else had an experience like this? Janet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com From JanKeeping <@t> gmail.com Wed Oct 6 13:04:11 2010 From: JanKeeping <@t> gmail.com (JanKeeping@gmail.com) Date: Wed Oct 6 13:04:15 2010 Subject: [Histonet] PAS staining In-Reply-To: <8280747504890101688@unknownmsgid> Message-ID: <005045014788c14e910491f69aca@google.com> The interesting thing about the test for Schiff reagent is that both texts (Freida Carson's and Sheehan Hrapchak) both specify 37-40% formaldehyde. Hence my confusion re; the color development On Oct 6, 2010 3:24pm, gayle callis wrote: > I was taught to avoid aqueous fixatives when trying to retain glycogen, > soluble in NBF. Carnoys, Gendres fluid or acid alcohol formalin are three > recommendations (Sheehan and Hrapchak Theory and Practice of > Histotechnology) followed by starting processing in 95 to 100% alcohols. > Alcoholic formalin should also work. It could very well be your long term > storage in NBF has removed the glycogen, although basement membranes or > fungus would not be affected. This was very apparent in a study done here > where they wanted to see glycogen storage in mouse livers fixed in NBF for > over a week and routinely processed starting in 70% alcohol. The glycogen, > for all purposes, was removed even in experimental animals who had large > quantity of glycogen in the cells (faintly stained but not what expected). > Certainly increasing time in periodic acid and Schiffs can help. Also, one > can increase the percentage of periodic acid from 0.5% to 1%, a hint > Culling > gave, as long as this is freshly made. > However, we never use periodic acid for fungus staining, only chromic acid > since periodic acid oxidation can give false negative results with Schiffs > reagent. This is published in J Histotechnology by Carson and Fredenburgh. > Interesting, but I still get a bright red pink color with Neutral buffered > formalin test. I have never used concentrated 37% to 40% stock > formaldehyde, only neutral buffered formalin (fixative) which would have > fewer aldehyde groups available. Outdated, bad Schiffs always has the > obvious purplish color with NBF. > One thing we never allow is return used Schiffs back into stock Schiffs. > Stock stain solutions are never contaminated with depleted, used > solutions. > We date when the Schiffs was used, and not reused within the week, this is > discarded. This was particularly important with human renal biopsy work > with > the renal pathologist recommending disposing of used Schiffs. Our biopsy > service did not handle a large number of biopsies in a year and this was a > way to ensure consistent PAS staining by using only Schiffs. Successful > PAS > staining of basement membrane on 1 to 2 um zinc formalin or FFPE sections > were never a problem. > Gayle M. Callis > HTL/HT/MT(ASCP) > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet > Keeping > Sent: Wednesday, October 06, 2010 10:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] PAS staining > I have for some time had a problem with Schiff's reagent and PAS staining. > - I have tested each new, unopened bottle of Schiff's reagent with > formaldehyde and always the color development was immediate, but purple, > definately not pink.This result has been quite consistant. > - PAS staining for glycogen using the method in *Histotechnology a self > Instructional text,* would fail to demonstrate any glycogen in autopsy > liver specimens. > I teach Histology at a community college and this problem has driven me > crazy for a number of years. I have tried several brands of Schiff with > the > same results. Recently I obtained sheep tissues which were promptly > refrigerated and fixed after death, and I had hoped these tissues would > demonstrate glycogen. ( My thinking was that perhaps delay before autopsy > was somehow diminishing the glycogen in the specimens that I had.or that > perhaps long term NBF fixation had hampered staining.) Basement membranes > were stained with the Schiff reagent as expected despite the purple color > in > the formaldehyde test. Hotchkiss Mcmanus with the same reagent also > produced > beautiful staining of fungus a lovely magenta color. A search of the web > made me suspiciious when I noted that Schiff added to 37-40% formaldehyde > should produce a pink or red color, however, A spot check of formalin > using > Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to > ask if he could make any recommendation. > Brian was not surprised by the purple color devopment in testing, He > suggested that a large number of available aldehyde groups would be > expected > to produce this color. He suggested that I increase my periodate oxidation > to 20-30 minutes and my Schiff application to 30 minutes. > This worked and I am extremely grateful!. Has anyone else had an > experience > like this? > Janet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________ Information from ESET Smart Security, version of virus > signature > database 5510 (20101006) __________ > The message was checked by ESET Smart Security. > http://www.eset.com > __________ Information from ESET Smart Security, version of virus > signature > database 5510 (20101006) __________ > The message was checked by ESET Smart Security. > http://www.eset.com > __________ Information from ESET Smart Security, version of virus > signature > database 5510 (20101006) __________ > The message was checked by ESET Smart Security. > http://www.eset.com From gayle.callis <@t> bresnan.net Wed Oct 6 13:05:18 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Oct 6 13:05:37 2010 Subject: [Histonet] PAS staining References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F43@EXCHMBC2.ad.ah.local> Message-ID: <001c01cb6581$0a97d3e0$1fc77ba0$@callis@bresnan.net> Sorry folks, hit the send button before finishing the message. As said previously, Jan is correct about temperature although RT can vary from lab to lab - ours has been tropical lately. Our Schiffs is brought from 4C to room temperature before staining and periodic acid is made up, fresh, in RT distilled water just before use. Unless a different temperature is specified in a staining protocol, room temperature is used for most special stains. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: gayle callis [mailto:gayle.callis@bresnan.net] Sent: Wednesday, October 06, 2010 11:58 AM To: 'Mahoney,Janice A'; 'Janet Keeping'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] PAS staining Jan is correct, and unless specified in a staining protocol, room temperature is used. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, October 06, 2010 11:20 AM To: 'Janet Keeping'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PAS staining Yes, I have experienced the same thing. Many people forget about the impact temperature has on some staining reactions. If you keep your Schiffs and Periodic acid in the refrigerator it may take longer to stain than it would if the reagents were at room temp. Many old procedures are written with the reagents at room temp (even the ones requiring refrigeration). This was one of those "live and learn" situations for me. Now temp and time are the first things I look at when a stain does not work on a known control. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: Wednesday, October 06, 2010 11:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS staining I have for some time had a problem with Schiff's reagent and PAS staining. - I have tested each new, unopened bottle of Schiff's reagent with formaldehyde and always the color development was immediate, but purple, definately not pink.This result has been quite consistant. - PAS staining for glycogen using the method in *Histotechnology a self Instructional text,* would fail to demonstrate any glycogen in autopsy liver specimens. I teach Histology at a community college and this problem has driven me crazy for a number of years. I have tried several brands of Schiff with the same results. Recently I obtained sheep tissues which were promptly refrigerated and fixed after death, and I had hoped these tissues would demonstrate glycogen. ( My thinking was that perhaps delay before autopsy was somehow diminishing the glycogen in the specimens that I had.or that perhaps long term NBF fixation had hampered staining.) Basement membranes were stained with the Schiff reagent as expected despite the purple color in the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced beautiful staining of fungus a lovely magenta color. A search of the web made me suspiciious when I noted that Schiff added to 37-40% formaldehyde should produce a pink or red color, however, A spot check of formalin using Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to ask if he could make any recommendation. Brian was not surprised by the purple color devopment in testing, He suggested that a large number of available aldehyde groups would be expected to produce this color. He suggested that I increase my periodate oxidation to 20-30 minutes and my Schiff application to 30 minutes. This worked and I am extremely grateful!. Has anyone else had an experience like this? Janet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com From JanKeeping <@t> gmail.com Wed Oct 6 13:06:05 2010 From: JanKeeping <@t> gmail.com (JanKeeping@gmail.com) Date: Wed Oct 6 13:06:10 2010 Subject: [Histonet] PAS staining In-Reply-To: <8280747504890101688@unknownmsgid> Message-ID: <00221538fc5e96ee3e0491f6a1d1@google.com> The interesting thing about the test for Schiff reagent is that both texts (Freida Carson's and Sheehan Hrapchak) both specify 37-40% formaldehyde. Hence my confusion re; the color development On Oct 6, 2010 3:24pm, gayle callis wrote: > I was taught to avoid aqueous fixatives when trying to retain glycogen, > soluble in NBF. Carnoys, Gendres fluid or acid alcohol formalin are three > recommendations (Sheehan and Hrapchak Theory and Practice of > Histotechnology) followed by starting processing in 95 to 100% alcohols. > Alcoholic formalin should also work. It could very well be your long term > storage in NBF has removed the glycogen, although basement membranes or > fungus would not be affected. This was very apparent in a study done here > where they wanted to see glycogen storage in mouse livers fixed in NBF for > over a week and routinely processed starting in 70% alcohol. The glycogen, > for all purposes, was removed even in experimental animals who had large > quantity of glycogen in the cells (faintly stained but not what expected). > Certainly increasing time in periodic acid and Schiffs can help. Also, one > can increase the percentage of periodic acid from 0.5% to 1%, a hint > Culling > gave, as long as this is freshly made. > However, we never use periodic acid for fungus staining, only chromic acid > since periodic acid oxidation can give false negative results with Schiffs > reagent. This is published in J Histotechnology by Carson and Fredenburgh. > Interesting, but I still get a bright red pink color with Neutral buffered > formalin test. I have never used concentrated 37% to 40% stock > formaldehyde, only neutral buffered formalin (fixative) which would have > fewer aldehyde groups available. Outdated, bad Schiffs always has the > obvious purplish color with NBF. > One thing we never allow is return used Schiffs back into stock Schiffs. > Stock stain solutions are never contaminated with depleted, used > solutions. > We date when the Schiffs was used, and not reused within the week, this is > discarded. This was particularly important with human renal biopsy work > with > the renal pathologist recommending disposing of used Schiffs. Our biopsy > service did not handle a large number of biopsies in a year and this was a > way to ensure consistent PAS staining by using only Schiffs. Successful > PAS > staining of basement membrane on 1 to 2 um zinc formalin or FFPE sections > were never a problem. > Gayle M. Callis > HTL/HT/MT(ASCP) > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet > Keeping > Sent: Wednesday, October 06, 2010 10:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] PAS staining > I have for some time had a problem with Schiff's reagent and PAS staining. > - I have tested each new, unopened bottle of Schiff's reagent with > formaldehyde and always the color development was immediate, but purple, > definately not pink.This result has been quite consistant. > - PAS staining for glycogen using the method in *Histotechnology a self > Instructional text,* would fail to demonstrate any glycogen in autopsy > liver specimens. > I teach Histology at a community college and this problem has driven me > crazy for a number of years. I have tried several brands of Schiff with > the > same results. Recently I obtained sheep tissues which were promptly > refrigerated and fixed after death, and I had hoped these tissues would > demonstrate glycogen. ( My thinking was that perhaps delay before autopsy > was somehow diminishing the glycogen in the specimens that I had.or that > perhaps long term NBF fixation had hampered staining.) Basement membranes > were stained with the Schiff reagent as expected despite the purple color > in > the formaldehyde test. Hotchkiss Mcmanus with the same reagent also > produced > beautiful staining of fungus a lovely magenta color. A search of the web > made me suspiciious when I noted that Schiff added to 37-40% formaldehyde > should produce a pink or red color, however, A spot check of formalin > using > Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to > ask if he could make any recommendation. > Brian was not surprised by the purple color devopment in testing, He > suggested that a large number of available aldehyde groups would be > expected > to produce this color. He suggested that I increase my periodate oxidation > to 20-30 minutes and my Schiff application to 30 minutes. > This worked and I am extremely grateful!. Has anyone else had an > experience > like this? > Janet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________ Information from ESET Smart Security, version of virus > signature > database 5510 (20101006) __________ > The message was checked by ESET Smart Security. > http://www.eset.com > __________ Information from ESET Smart Security, version of virus > signature > database 5510 (20101006) __________ > The message was checked by ESET Smart Security. > http://www.eset.com > __________ Information from ESET Smart Security, version of virus > signature > database 5510 (20101006) __________ > The message was checked by ESET Smart Security. > http://www.eset.com From ander093 <@t> umn.edu Wed Oct 6 13:25:28 2010 From: ander093 <@t> umn.edu (LuAnn Anderson) Date: Wed Oct 6 13:25:33 2010 Subject: [Histonet] PAS staining In-Reply-To: References: Message-ID: <4CACBF18.1030709@umn.edu> I still make my own Schiff's reagent. It is easy to make and I've never had a problem with it. Keeps well in refrigerator. I do make the periodic acid fresh each time. If anyone wants the recipe, let me know. LuAnn On 10/6/2010 11:43 AM, Janet Keeping wrote: > I have for some time had a problem with Schiff's reagent and PAS staining. > > - I have tested each new, unopened bottle of Schiff's reagent with > formaldehyde and always the color development was immediate, but purple, > definately not pink.This result has been quite consistant. > - PAS staining for glycogen using the method in *Histotechnology a self > Instructional text,* would fail to demonstrate any glycogen in autopsy > liver specimens. > > I teach Histology at a community college and this problem has driven me > crazy for a number of years. I have tried several brands of Schiff with the > same results. Recently I obtained sheep tissues which were promptly > refrigerated and fixed after death, and I had hoped these tissues would > demonstrate glycogen. ( My thinking was that perhaps delay before autopsy > was somehow diminishing the glycogen in the specimens that I had.or that > perhaps long term NBF fixation had hampered staining.) Basement membranes > were stained with the Schiff reagent as expected despite the purple color in > the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced > beautiful staining of fungus a lovely magenta color. A search of the web > made me suspiciious when I noted that Schiff added to 37-40% formaldehyde > should produce a pink or red color, however, A spot check of formalin using > Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to > ask if he could make any recommendation. > > Brian was not surprised by the purple color devopment in testing, He > suggested that a large number of available aldehyde groups would be expected > to produce this color. He suggested that I increase my periodate oxidation > to 20-30 minutes and my Schiff application to 30 minutes. > > This worked and I am extremely grateful!. Has anyone else had an experience > like this? > Janet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Wed Oct 6 14:42:11 2010 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Wed Oct 6 14:42:15 2010 Subject: [Histonet] Question about Oil Red O controls In-Reply-To: References: Message-ID: <9404D5E5-AD1D-442C-9801-8E1C40B78019@email.arizona.edu> Komal, I don't know what kind of lab you are in, I'm in a core facility and I do histology on research projects. When I get an ORO this is what I do for a control: I get a piece of tissue like mouse kidney with some fat attached or maybe some muscle with fat and have it snap frozen. I have found that the frozen blocks stay good for a long time at -80?C and so do the frozen sections on slides. I always cut a bunch of slides and store them and take one out when I have the stain ordered. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Oct 6, 2010, at 6:15 AM, Komal Gada wrote: > Hello Histonetters, > > I am trying to find a procedure for using butter and egg yolks as > controls > for the Oil Red O stain (to show the fat). > > Does anyone have something they would be able to share with me? > > Thanks, > Komal > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mcauliff <@t> umdnj.edu Wed Oct 6 15:28:23 2010 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Oct 6 15:25:34 2010 Subject: [Histonet] PAS STAIN In-Reply-To: References: Message-ID: <4CACDBE7.1010203@umdnj.edu> I make my Schiff's from scratch and store it in the refrigerator. I use a minimum amount of charcoal and I heat it in the oven at over 100 C the night before to be sure it is nice and dry. I make periodic acid fresh that day. I make bisulfite rinses fresh that day. Aqueous formalin for 48 hours at room temp. is OK for rat and mouse liver glycogen, I don't know about other species. Slices of liver should be thin. When in doubt formalin+alcohol+acetic acid is an excellent fixative. Geoff Diana McCaig wrote: > Hi > We have been doing a PAS stain on the same control block and same > reagent supplier for a long time. > Yesterday when we ran the slides, they failed to stain > > We opened new bottles of Periodic Acid and Schiff's Reagent and recut > fresh controls > > Still, we are unable to get any staining. > > Suggestions to help would be appreciated > > Diana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From sgoebel <@t> xbiotech.com Wed Oct 6 15:31:25 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Oct 6 15:31:30 2010 Subject: [Histonet] PAS STAIN Message-ID: <20101006133125.9e2d9aa830e8449a2412eb1e4f2f067e.36c961be12.wbe@email04.secureserver.net> I know there were a ton of posts about this, but I deleted them o accident...Did you test your Schiff's? Put a drop of 10% formalin i n it...if it turns pink, it's good...if not, throw it out =) Sarah Goebel Histotechnician XBiotech US 8201 East Riverside Dr. Bldg 4 Suite 100 A (512)386-5107 -------- Original Message -------- Subject: Re: [Histonet] PAS STAIN From: Geoff McAuliffe <[1]mcauliff@um Date: Wed, October 06, 2010 1:28 pm To: Diana McCaig <[2]dmccaig@ckha.on. <[3]histonet@lists.uts I make my Schiff's from scratch and store it in the refrigerator. I use a minimum amount of charcoal and I heat it in the oven at over 100 C the night before to be sure it is nice and dry. I make periodic acid fresh that day. I make bisulfite rinses fresh that day. Aqueous formalin for 48 hours at room temp. is OK for rat and mouse liver glycogen, I don't know about other species. Slices of liver should be thin. When in doubt formalin+alcohol+acetic acid is an excellent fixative. Geoff Diana McCaig wrote: > Hi > We have been doing a PAS stain on the same control block and same > reagent supplier for a long time. > Yesterday when we ran the slides, they failed to stain > > We opened new bottles of Periodic Acid and Schiff's Reagent and recut< > > Still, we are unable to get any staining. > > Suggestions to help would be appreciated > > Diana > _______________________________________________ > Histonet mailing list > [4]Histonet@lists.ut > [5] > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 [6]mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list [7]Histonet@lists.utsouth [8]http: References 1. 3D"mailto:mcauliff@umdnj.edu" 2. 3D"mailto:dmccaig@ckha.on.ca" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:Histonet@lists.utsouthwestern.edu" 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 6. 3D"mailto:mcauliff@umdnj.edu" 7. 3D"mailto:Histonet@lists.utsouthwestern.edu" 8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From sgoebel <@t> xbiotech.com Wed Oct 6 16:17:28 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Oct 6 16:17:36 2010 Subject: [Histonet] IL1a Message-ID: <20101006141728.9e2d9aa830e8449a2412eb1e4f2f067e.c522e83ed7.wbe@email04.secureserver.net> Has anybody ever worked with IL1-alpha for IHC? Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 From AnthonyH <@t> chw.edu.au Wed Oct 6 18:16:46 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Oct 6 18:16:59 2010 Subject: [Histonet] PAS staining In-Reply-To: Message-ID: Janet, The major problem I have encounted with the PAS stain is breakdown of the Schiff's reagent (white precipitate). Replacement of the Schiff's reagent usually (?always) solves this. I am not surprised that glycogen in autopsy tissues is difficult to demonstrate. Post-mortem seems to decrease the glycogen levels. I prefer to use bowel and kidney (mucin & basement membranes) to initially check the solutions. Interestingly we include glycogen-rich liver in our PAS control block and if you audit the PAS controls from when the Schiff's bottle is opened until it is empty, or when staining decreases, you will notice a gradual "loss" of glycogen staining. Decrease in PAS staining of Fungi and basement membranes is often not as apparent. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Keeping Sent: Thursday, 7 October 2010 3:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS staining I have for some time had a problem with Schiff's reagent and PAS staining. - I have tested each new, unopened bottle of Schiff's reagent with formaldehyde and always the color development was immediate, but purple, definately not pink.This result has been quite consistant. - PAS staining for glycogen using the method in *Histotechnology a self Instructional text,* would fail to demonstrate any glycogen in autopsy liver specimens. I teach Histology at a community college and this problem has driven me crazy for a number of years. I have tried several brands of Schiff with the same results. Recently I obtained sheep tissues which were promptly refrigerated and fixed after death, and I had hoped these tissues would demonstrate glycogen. ( My thinking was that perhaps delay before autopsy was somehow diminishing the glycogen in the specimens that I had.or that perhaps long term NBF fixation had hampered staining.) Basement membranes were stained with the Schiff reagent as expected despite the purple color in the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced beautiful staining of fungus a lovely magenta color. A search of the web made me suspiciious when I noted that Schiff added to 37-40% formaldehyde should produce a pink or red color, however, A spot check of formalin using Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to ask if he could make any recommendation. Brian was not surprised by the purple color devopment in testing, He suggested that a large number of available aldehyde groups would be expected to produce this color. He suggested that I increase my periodate oxidation to 20-30 minutes and my Schiff application to 30 minutes. This worked and I am extremely grateful!. Has anyone else had an experience like this? Janet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From amosbrooks <@t> gmail.com Wed Oct 6 18:21:13 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Oct 6 18:21:17 2010 Subject: [Histonet] HMGB Message-ID: Hi, Sorry about taking so long to get back to you. The antibody I'm using is from abcam, cat# ab18256. I really think the 1:100 or 1:200 is the best dilution so far. Amos On Mon, Oct 4, 2010 at 1:01 PM, wrote: > Message: 1 > Date: Mon, 4 Oct 2010 08:21:15 -0500 > From: Fabrice GANKAM > Subject: Re: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies > To: Amos Brooks > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > thanks Amos > which one did you used ? you have catalog number ? > From sfeher <@t> CMC-NH.ORG Wed Oct 6 18:46:25 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Oct 6 18:46:29 2010 Subject: [Histonet] Question about Oil Red O controls In-Reply-To: <9404D5E5-AD1D-442C-9801-8E1C40B78019@email.arizona.edu> References: <9404D5E5-AD1D-442C-9801-8E1C40B78019@email.arizona.edu> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F6BC@exchange.cmc-nh.org> Believe it or not, mayonnaise makes a great control for Oil Red O. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, October 06, 2010 3:42 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question about Oil Red O controls Komal, I don't know what kind of lab you are in, I'm in a core facility and I do histology on research projects. When I get an ORO this is what I do for a control: I get a piece of tissue like mouse kidney with some fat attached or maybe some muscle with fat and have it snap frozen. I have found that the frozen blocks stay good for a long time at -80?C and so do the frozen sections on slides. I always cut a bunch of slides and store them and take one out when I have the stain ordered. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Oct 6, 2010, at 6:15 AM, Komal Gada wrote: > Hello Histonetters, > > I am trying to find a procedure for using butter and egg yolks as > controls for the Oil Red O stain (to show the fat). > > Does anyone have something they would be able to share with me? > > Thanks, > Komal > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From travers.3 <@t> osu.edu Thu Oct 7 07:44:31 2010 From: travers.3 <@t> osu.edu (Susan Travers) Date: Thu Oct 7 07:44:35 2010 Subject: [Histonet] chicken EGFP aby In-Reply-To: References: Message-ID: <04A71EFE05FE5F4DAA05D0500FD05970035B9499@Distal.dentnet.dent.ohio-state.edu> Does anyone have experience with detecting EGFP with some of the current aby's made in chicken? We have used aby's from both AVES labs and Millipore with negative results. In both cases we used the biotinylated secondary aby from AVES followed by a fluorescent streptavidin. Absolutely no staining. Using the same tissue, we were able to use a different aby made in rabbit and it worked great. However, because of double-labeling needs we'd really like to get the chicken to work. Perhaps someone has experience or insights? Thanks! Susan Travers Division of Oral Biology College of Dentistry The Ohio State University From anonwums1 <@t> gmail.com Thu Oct 7 08:23:16 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Oct 7 08:23:20 2010 Subject: [Histonet] chicken EGFP aby In-Reply-To: <04A71EFE05FE5F4DAA05D0500FD05970035B9499@Distal.dentnet.dent.ohio-state.edu> References: <04A71EFE05FE5F4DAA05D0500FD05970035B9499@Distal.dentnet.dent.ohio-state.edu> Message-ID: I've had good luck using Abcam's chicken polyclonal: http://www.abcam.com/GFP-antibody-ab13970.html I use a directly conjugated secondary, and the staining is usually pretty bright. But then again, my GFP expression is high. Adam On Thu, Oct 7, 2010 at 7:44 AM, Susan Travers wrote: > Does anyone have experience with detecting EGFP with some of the current > aby's made in chicken? We have used aby's from both AVES labs and Millipore > with negative results. In both cases we used the biotinylated secondary aby > from AVES followed by a fluorescent streptavidin. Absolutely no staining. > > Using the same tissue, we were able to use a different aby made in rabbit > and it worked great. However, because of double-labeling needs we'd really > like to get the chicken to work. > > Perhaps someone has experience or insights? > > Thanks! > Susan Travers > Division of Oral Biology > College of Dentistry > The Ohio State University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From CarterK <@t> MedImmune.com Thu Oct 7 08:24:23 2010 From: CarterK <@t> MedImmune.com (Carter, Kendra) Date: Thu Oct 7 08:24:55 2010 Subject: [Histonet] Training In-Reply-To: References: Message-ID: Does anyone know of any GLP training in El Paso, TX? Kendra Leigh Carter -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, October 06, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 83, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Helicobacter pylori (Laurie Colbert) 2. RE: AChE fiber staining protocoll? (szigcs@bio.u-szeged.hu) 3. RE: Helicobacter pylori (Weems, Joyce) 4. PAS STAIN (Diana McCaig) 5. Histokinette 2000 (Biedermann, JoAnn) 6. RE: PAS STAIN (Rathborne, Toni) 7. Query Special Stainers (Joanne Clark) 8. Re: Helicobacter pylori (Brandi Higgins) 9. AW: [Histonet] PAS STAIN (Gudrun Lang) 10. Where to buy Michels RE: [Histonet] DIF Transport Media (gayle callis) 11. RE: Query Special Stainers (Mahoney,Janice A) 12. RE: PAS STAIN (gayle callis) 13. RE: Query Special Stainers (Marcia Funk) 14. PAS staining (Janet Keeping) ---------------------------------------------------------------------- Message: 1 Date: Wed, 6 Oct 2010 07:48:25 -0700 From: "Laurie Colbert" Subject: RE: [Histonet] Helicobacter pylori To: "Kathy M. Gorham" , Message-ID: <57BE698966D5C54EAE8612E8941D768309A5ACBC@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" We basically do a dif quik stain. I buy a kit called "Three Step Stain" from Cardinal. The total staining process takes about 1 minute. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, October 06, 2010 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter pylori Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 06 Oct 2010 17:00:35 +0200 From: szigcs@bio.u-szeged.hu Subject: RE: [Histonet] AChE fiber staining protocoll? To: "Houston, Ronald" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <20101006170035.j2waztulso4480gg@webmail.u-szeged.hu> Content-Type: text/plain; charset=ISO-8859-2; DelSp="Yes"; format="flowed" Id??zet ("Houston, Ronald" ): Thank you for the answer. We will try this protocol and i will reply the results. Csaba Szigeti > I have had great success in fine fibers with the modification of > Martucciello et al, Eur J Pediatr Surg 2001; 11: 300-304, but only > in 15 micron intestine sections, so I do not know how it will > transpose to brain studies and the thicker sections > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > szigcs@bio.u-szeged.hu > Sent: Wednesday, October 06, 2010 3:34 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AChE fiber staining protocoll? > > Dear Histonet Members! > > We are going to investigate AChE fiber density changes in the cortex > of transcardially perfused rat brain slices (30 micrometer, criostat > sections, perfusing solution: 4% formaldehyde in PB ph 7.4). We need a > protocoll to visualize FINE fiber structure to be able to count changes. > > We used modification of Hedreen and original Tago protocoll. Hedreen > good results for general, but no fiber staining, Tago no sucsses. > > Could you please tell us some advise, protocolls functioning on teh > above mentioned objects? > > Thank you all in advance... > Csaba Szigeti > > ---------------------------------------------------------------- > This message was sent using IMP, the Internet Messaging Program. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. ------------------------------ Message: 3 Date: Wed, 6 Oct 2010 11:01:52 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Helicobacter pylori To: "Kathy M. Gorham" , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640398777983@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We use what we call a modified Genta. That is a modified Steiner if done manually. Because we have the Artisan and DAKO didn't have a Genta kit, I tried using the Warthin Starry and adding the Alcian blue and H&E manually and it worked fine. So we have not renamed it a modified Warthin Starry! But the H. pylori are very visible as well as the mucin and the morphology. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, October 06, 2010 10:20 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter pylori Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Wed, 6 Oct 2010 11:03:05 -0400 From: "Diana McCaig" Subject: [Histonet] PAS STAIN To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana ------------------------------ Message: 5 Date: Wed, 6 Oct 2010 10:04:55 -0500 From: "Biedermann, JoAnn" Subject: [Histonet] Histokinette 2000 To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <833890C7E1BE584B926A2584E6B37ADC6B03F42E@MAIL2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" I have a Leica Histokinette 2000 that needs one beaker too be usable. Does anyone know where I can buy one of these? IMEB, inc does not have them. Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173 Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 JABiedermann@uams.edu Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ------------------------------ Message: 6 Date: Wed, 6 Oct 2010 11:06:12 -0400 From: "Rathborne, Toni" Subject: RE: [Histonet] PAS STAIN To: "Diana McCaig" , Message-ID: Content-Type: text/plain; charset="utf-8" Were the new bottles from the same lot? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Wednesday, October 06, 2010 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 7 Date: Wed, 6 Oct 2010 09:22:12 -0600 From: "Joanne Clark" Subject: [Histonet] Query Special Stainers To: Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01375230@mail.pcnm.com> Content-Type: text/plain; charset="us-ascii" Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 8 Date: Wed, 6 Oct 2010 11:27:59 -0400 From: Brandi Higgins Subject: Re: [Histonet] Helicobacter pylori To: "Kathy M. Gorham" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, We use what is pretty much a diff quik stain, the stain is called QW and it is from poly scientific. The procedure is really easy. Deparaffinize, alcohols, methanol, water and then we do 15 slow dips in the QW2, 15 slow dips in the QW3, wash in water, 2 quick dips in alcohol and then dry in the oven, coverslip. Depending on your pathologist's preference you may need to adjust the dips in QW3 or the dips in the alcohol (which will decolorize slightly). Hope this helps! Brandi Higgins, BS, HT(ASCP) On Wed, Oct 6, 2010 at 10:19 AM, Kathy M. Gorham wrote: > Good Morning Histo land. I would like to know what others are using for > a special stain for Helicobacter pylori not IHC. Do you use a kit? From > where? Make up your own? Procedure. Thanks you. You have always been > so helpful. Kathy Gorham H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > Financial Excellence Award 2010 awarded by the national Rural Health > Research & Policy Analysis Center > Healthcare Achievement Award for Quality in Patient Care Delivery and > Satisfaction 2010 awarded by Amerinet > > GRH Mission > We will ensure access to high-quality, cost-effective health services in a > safe, customer-friendly environment for all those in need of our services. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s > only > and should be protected against viewing by unauthorized persons. The > information > herein may have been disclosed from records whose confidentiality is > protected > by Federal and State Law. Federal regulations prohibit further distribution > or > copying of this information without permission. If you received this > e-mail > transmission in error, please notify the sender immediately to arrange for > return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Wed, 6 Oct 2010 17:39:03 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] PAS STAIN To: "'Diana McCaig'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <986B2C6B449344EBAECEB4080A6E542D@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" You can test the activity of the Schiffs. Add a few drops of formalin to a small amount of Schiffs. There should be the typical pink stain. The pH should be about 2. If there are white flakes in the bottle, this could be a sign for a too high pH. Is your periodic acid ok? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Diana McCaig Gesendet: Mittwoch, 06. Oktober 2010 17:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 6 Oct 2010 09:40:17 -0600 From: "gayle callis" Subject: Where to buy Michels RE: [Histonet] DIF Transport Media To: "'Tony Henwood'" , "'Della Speranza, Vinnie'" , "'kristen arvidson'" , "'histonet'" Message-ID: <002801cb656c$c8bd8cd0$5a38a670$@callis@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" Dear Tony, Thank you for the fine reply on Michel's Transport Media. We had excellent results with human renal biopsies destined for immunofluorescence staining. I don't recall ever exceeding 48 hours in Michels as 72 hours was allowed per recommendation from the original and Elias's publications. The kidney morphology didn't suffer excessively from this transport media as seen on our H&E stained frozen sections from Liquid Nitrogen cooled isopentane snap frozen needle biopsies. My renal pathologist always commented that the frozen section H&E looked very much like his the FFPE H&E section. This must vary from laboratory to laboratory and also what tissue was transported e.g. skin biopsies versus kidney biopsies. We always were very careful with good Michels buffer rinses. As for where to access Michels Transport Media and Michels buffer, we purchased these from Poly Scientific in much larger volumes at a cheaper price than Wampole(?)(Zeus). Aliquots were made and distributed to laboratories although that may not be ideal since busy laboratories may find this a bit labor intensive. I know of laboratories who make up Michels with great success. As for transporting tissue on saline soaked gauze, I can't stress the importance enough to NEVER let the tissue dry out, and snap freeze it as soon as it arrives in the laboratory. I would prefer receiving a tissue in cell culture fluid to ensure no drying. The reference is much appreciated. G'day and missed seeing you at NSH symposium/convention this year. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, October 05, 2010 5:44 PM To: Della Speranza, Vinnie; kristen arvidson; histonet Subject: RE: [Histonet] DIF Transport Media Vinnie, I hope you are well. The following might be of use: Specimens for immunofluorescence are usually submitted to the laboratory in cell culture fluid (eg Hanks or RPMI) or on saline soaked gauze. For transport to other institutions, Michel's Transport Medium has often been used: Michel's Buffer 1M potassium citrate, pH 7.0 2.5 ml 0.1M magnesium sulphate 5.0 ml 0.1M N ethylmalemide 5.0 ml Distilled H2O 87.5 ml * Mix well and store at 2 8oC. Exp. 1 year Michel's Transport Medium Michel's Buffer 100ml Ammonium sulphate 55gm Adjust pH to 7.0 7.4 with 1M KOH. Store at 2 8oC. Exp. 1 year Unfortunately Michel's Transport Medium has erroneously been called Michel's Fixative. None of the components of Michel's Transport Medium is a fixative. Ammonium sulphate precipitates antigen-antibody complexes in diseased skin and renal tissues. N ethylmalemide modifies free sulphydryl groups of cysteine residues in proteins (Fischer 2006). Michel's Transport Medium has been shown to be deleterious to morphology. Ultrastructurally, complete destruction of plasma membranes and intracytoplasmic organelles occurs after 48 hours storage. On the other hand, antigenicity is well preserved even after many days storage (Fischer 2006). Fischer (2006) Intern J Surg Pathol 14(1):108. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Wednesday, 6 October 2010 6:10 AM To: 'kristen arvidson'; histonet Subject: RE: [Histonet] DIF Transport Media Zeus was a company that used to market michel's under their own label. I don't believe zeus still exists. Michel's is the name associated with the author of the original paper. I don't have the reference. This solution does not require refrigeration. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Monday, October 04, 2010 2:30 PM To: histonet Subject: [Histonet] DIF Transport Media Is Michel Medium the same as Zeus?? Do these need to be refrigerated? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com ------------------------------ Message: 11 Date: Wed, 6 Oct 2010 10:49:22 -0500 From: "Mahoney,Janice A" Subject: [Histonet] RE: Query Special Stainers To: 'Joanne Clark' , "histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F40@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" We have 2 Ventana special stainers and they are very reliable work horses. They run every day over half the day and put out very consistent results. Very user friendly too. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, October 06, 2010 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Query Special Stainers Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 12 Date: Wed, 6 Oct 2010 10:04:44 -0600 From: "gayle callis" Subject: RE: [Histonet] PAS STAIN To: "'Rathborne, Toni'" , "'Diana McCaig'" , Message-ID: <003401cb6570$33037c00$990a7400$@callis@bresnan.net> Content-Type: text/plain; charset="utf-8" I suspect bad Periodic acid. We never buy periodic acid already in solution and if that has been sitting around even in a kit, it may have gone bad. In a workshop taught by Charles Culling, an expert on PAS staining, he stressed making periodic acid fresh daily or each time the stain is done. It is not expensive, goes into solution readily, then toss the periodic acid to never be reused with exception of that one day. Also, you can test your Schiffs reagent by putting a few drops of Schiffs into 10 mls NBF, watch it turn a very bright pink red instantly. If it turns purplish, it is not good. It may be a bad kit, but you never said you were using a kit only "new bottles of ....." Consequently we buy periodic acid in crystalline form, make up 1% Periodic acid, and buy the Schiffs Reagent from Fisher, never a kit. Sigma also sells good Schiffs, but Fisher Scientific aka Thermo has larger quantity for less price. We also store our Schiffs in the refrigerator rather than room temperature, a habit left over from days when we made this reagent up in house. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, October 06, 2010 9:06 AM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PAS STAIN Were the new bottles from the same lot? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Wednesday, October 06, 2010 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com ------------------------------ Message: 13 Date: Wed, 06 Oct 2010 12:08:04 -0400 From: "Marcia Funk" Subject: [Histonet] RE: Query Special Stainers To: "Janice A Mahoney" , "histonet@lists.utsouthwestern.edu" , "'Joanne Clark'" Message-ID: <4CAC5893.9B87.00AC.0@mercyhealth.com> Content-Type: text/plain; charset=US-ASCII We also have 2 Vantnan Special stainers and totally agree. Marcia M mason City Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> "Mahoney,Janice A" 10/06/2010 10:49 AM >>> We have 2 Ventana special stainers and they are very reliable work horses. They run every day over half the day and put out very consistent results. Very user friendly too. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, October 06, 2010 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Query Special Stainers Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 6 Oct 2010 14:13:23 -0230 From: Janet Keeping Subject: [Histonet] PAS staining To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I have for some time had a problem with Schiff's reagent and PAS staining. - I have tested each new, unopened bottle of Schiff's reagent with formaldehyde and always the color development was immediate, but purple, definately not pink.This result has been quite consistant. - PAS staining for glycogen using the method in *Histotechnology a self Instructional text,* would fail to demonstrate any glycogen in autopsy liver specimens. I teach Histology at a community college and this problem has driven me crazy for a number of years. I have tried several brands of Schiff with the same results. Recently I obtained sheep tissues which were promptly refrigerated and fixed after death, and I had hoped these tissues would demonstrate glycogen. ( My thinking was that perhaps delay before autopsy was somehow diminishing the glycogen in the specimens that I had.or that perhaps long term NBF fixation had hampered staining.) Basement membranes were stained with the Schiff reagent as expected despite the purple color in the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced beautiful staining of fungus a lovely magenta color. A search of the web made me suspiciious when I noted that Schiff added to 37-40% formaldehyde should produce a pink or red color, however, A spot check of formalin using Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to ask if he could make any recommendation. Brian was not surprised by the purple color devopment in testing, He suggested that a large number of available aldehyde groups would be expected to produce this color. He suggested that I increase my periodate oxidation to 20-30 minutes and my Schiff application to 30 minutes. This worked and I am extremely grateful!. Has anyone else had an experience like this? Janet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 83, Issue 6 *************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From srishan <@t> mail.holyname.org Thu Oct 7 09:32:13 2010 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Thu Oct 7 09:32:37 2010 Subject: [Histonet] problems with staining IHC Message-ID: Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From settembr <@t> umdnj.edu Thu Oct 7 09:41:59 2010 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Thu Oct 7 09:42:14 2010 Subject: [Histonet] problems with staining IHC In-Reply-To: References: Message-ID: Are you staining by hand or are you automated? Dana Settembre University Hospital Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Thursday, October 07, 2010 10:32 AM To: histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Oct 7 09:47:45 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 7 09:48:02 2010 Subject: [Histonet] problems with staining IHC In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390EB2133FC2@IBMB7Exchange.digestivespecialists.com> Hi Mala, I had this same problem. It was solved by adding Tween to the buffer. It made the reagents flow much better over the slides. Also before you stain, put the slides in a rinse of buffer/tween and agitate them for about 30 seconds. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Thursday, October 07, 2010 10:32 AM To: histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Oct 7 09:55:58 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Oct 7 09:56:02 2010 Subject: [Histonet] problems with staining IHC In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E273839A014@UWHC-MAIL01.uwhis.hosp.wisc.edu> What kind of stainers are you using and is it happening on all your stainers? Or are you staining by hand? Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Thursday, October 07, 2010 9:32 AM To: histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Thu Oct 7 10:18:10 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Oct 7 10:18:17 2010 Subject: [Histonet] problems with staining IHC References: Message-ID: <09C6DAC9CD014952B08597927EDA288A@auxs.umn.edu> Mala, If your main problem is that the reagents aren't spreading across your slides, I agree that you should put Tween 20 (0.05% per volume) in all of your rinse buffers between steps, regardless of if you do your IHC stains manually or with an autostainer. If this doesn't solve the problem, post your method on histonet so that we know more about your procedure steps and can send additional advice. Jan Shivers UMN Vet Diag Lab ----- Original Message ----- From: To: ; Sent: Thursday, October 07, 2010 9:32 AM Subject: [Histonet] problems with staining IHC > Hi All, > > Our pathologists have made a complain that the staining of slides have > been a problem. Same antibodies which stain one day does not work the > following day. This has been going on for a few months. When we repeat > stain they either work or not work and we have been sending them to > reference labs. > > Our tech support person was here and told us that the slides ( fisher plus > slides) have been a problem with some of her customers. Meanwhile our > surrounding labs which are using the same slides have no issues. She > showed us how the reagents were not spreading properly on the slides. > > Is anyone confronted this issue? If so, how was it solved? > > Thanks in advance. > > Mala Srishan > Supervisor, Histology > Holy Name Medical Center > Teaneck NJ 07666 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > Holy Name Medical Center is the recipient of: > > Magnet Recognition for Excellence in Patient Care, American Nurses > Credentialing Center > > 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern > Healthcare > > Best Places to Work in New Jersey, NJBIZ > > Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. > Power > > Distinguished Hospital Awards for Clinical Excellence, HealthGrades > > Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, > HealthGrades > > Best in Value Award, Data Advantage, LLC > > Chest Pain Center Accreditation, Society of Chest Pain Centers > > Primary Stroke Center Designation, The Joint Commission and NJ Department > of Health and Human Services > > > **** Warning: The information contained in this message is privileged and > CONFIDENTIAL and is intended only for the use of the addressee above. If > you are not the intended recipient, you are hereby notified that any > disclosure, copying, distribution, or taking of any action in reliance on > the content of this message is strictly prohibited. If you have received > this communication in error, please notify the sender by replying to this > message, and then delete it from your system. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From higginst <@t> amapath.com Thu Oct 7 10:52:43 2010 From: higginst <@t> amapath.com (Tim Higgins) Date: Thu Oct 7 10:57:19 2010 Subject: [Histonet] problems with staining IHC In-Reply-To: References: Message-ID: <000701cb6637$aeccc180$6a03a8c0@apg> Hi Mala, We've used the Fisher superfrost plus slides for years and have never had that issue, it sounds more like you need to add some Tween to your buffer. That will help the solutions spread along your slide. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Thursday, October 07, 2010 9:32 AM To: histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kcruise <@t> path.wustl.edu Thu Oct 7 11:09:20 2010 From: kcruise <@t> path.wustl.edu (Cruise, Karen) Date: Thu Oct 7 11:09:50 2010 Subject: [Histonet] Equipment Purchase Questions Message-ID: <6428E3A48152704F838E99641B0973FA2EBF9A@PATHEXCH.wusm-path.wustl.edu> Hello Histo Community, We are currently looking to purchase several items. I'm hoping someone can shed light on whether or not we are headed in the right direction. Processor: Initially we were leaning towards the Leica ASP 300, now I'm wondering if we are about to purchase to much processor for the needs of our lab. We process maybe 50 blocks per month. We are unable to use a microwave processor. We process about 95% breast tissue. Someone mentioned the TP1020. Has anyone any comments on this processor. We are also looking to purchase a ph meter and a fume adsorber, any recommendations ? Your responses will be greatly appreciated as we are looking to purchase before the end of the month. Thanks for all your help and suggestions, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcruise@path.wustl.edu From sfeher <@t> CMC-NH.ORG Thu Oct 7 11:21:03 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Thu Oct 7 11:21:08 2010 Subject: [Histonet] Equipment Purchase Questions In-Reply-To: <6428E3A48152704F838E99641B0973FA2EBF9A@PATHEXCH.wusm-path.wustl.edu> References: <6428E3A48152704F838E99641B0973FA2EBF9A@PATHEXCH.wusm-path.wustl.edu> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F6C9@exchange.cmc-nh.org> Karen, I would highly recommend Leica's Peloris processor. We use it for rapid tissue processing (2 hour processing time for cores and small specimens). It gives us the option of using one retort for rapid processing and the other for more conventional processing. We are also saving on reagents since we do not have to change out the entire processor weekly or twice per week. Peloris keeps track of the reagents and lets us know when one of them needs to be changed. We have been using our two units for a little over 9 months and we have yet to have anything either over or under processed. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cruise, Karen Sent: Thursday, October 07, 2010 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment Purchase Questions Hello Histo Community, We are currently looking to purchase several items. I'm hoping someone can shed light on whether or not we are headed in the right direction. Processor: Initially we were leaning towards the Leica ASP 300, now I'm wondering if we are about to purchase to much processor for the needs of our lab. We process maybe 50 blocks per month. We are unable to use a microwave processor. We process about 95% breast tissue. Someone mentioned the TP1020. Has anyone any comments on this processor. We are also looking to purchase a ph meter and a fume adsorber, any recommendations ? Your responses will be greatly appreciated as we are looking to purchase before the end of the month. Thanks for all your help and suggestions, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcruise@path.wustl.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Thu Oct 7 11:23:41 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Thu Oct 7 11:23:50 2010 Subject: [Histonet] Equipment Purchase Questions In-Reply-To: <73A7ED895EE0C24D9267ED814911DF191773F6C9@exchange.cmc-nh.org> References: <6428E3A48152704F838E99641B0973FA2EBF9A@PATHEXCH.wusm-path.wustl.edu> <73A7ED895EE0C24D9267ED814911DF191773F6C9@exchange.cmc-nh.org> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F4E@EXCHMBC2.ad.ah.local> I completely agree with Steve. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Thursday, October 07, 2010 11:21 AM To: Cruise, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Equipment Purchase Questions Karen, I would highly recommend Leica's Peloris processor. We use it for rapid tissue processing (2 hour processing time for cores and small specimens). It gives us the option of using one retort for rapid processing and the other for more conventional processing. We are also saving on reagents since we do not have to change out the entire processor weekly or twice per week. Peloris keeps track of the reagents and lets us know when one of them needs to be changed. We have been using our two units for a little over 9 months and we have yet to have anything either over or under processed. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cruise, Karen Sent: Thursday, October 07, 2010 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment Purchase Questions Hello Histo Community, We are currently looking to purchase several items. I'm hoping someone can shed light on whether or not we are headed in the right direction. Processor: Initially we were leaning towards the Leica ASP 300, now I'm wondering if we are about to purchase to much processor for the needs of our lab. We process maybe 50 blocks per month. We are unable to use a microwave processor. We process about 95% breast tissue. Someone mentioned the TP1020. Has anyone any comments on this processor. We are also looking to purchase a ph meter and a fume adsorber, any recommendations ? Your responses will be greatly appreciated as we are looking to purchase before the end of the month. Thanks for all your help and suggestions, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcruise@path.wustl.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From mpence <@t> grhs.net Thu Oct 7 11:36:00 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Oct 7 11:36:07 2010 Subject: [Histonet] Equipment Purchase Questions In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F4E@EXCHMBC2.ad.ah.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974A47@is-e2k3.grhs.net> I third that! I have had my Peloris for about 2 years. The key is thin, uniform sections. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Thursday, October 07, 2010 11:24 AM To: 'Feher, Stephen'; Cruise, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Equipment Purchase Questions I completely agree with Steve. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Thursday, October 07, 2010 11:21 AM To: Cruise, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Equipment Purchase Questions Karen, I would highly recommend Leica's Peloris processor. We use it for rapid tissue processing (2 hour processing time for cores and small specimens). It gives us the option of using one retort for rapid processing and the other for more conventional processing. We are also saving on reagents since we do not have to change out the entire processor weekly or twice per week. Peloris keeps track of the reagents and lets us know when one of them needs to be changed. We have been using our two units for a little over 9 months and we have yet to have anything either over or under processed. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cruise, Karen Sent: Thursday, October 07, 2010 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment Purchase Questions Hello Histo Community, We are currently looking to purchase several items. I'm hoping someone can shed light on whether or not we are headed in the right direction. Processor: Initially we were leaning towards the Leica ASP 300, now I'm wondering if we are about to purchase to much processor for the needs of our lab. We process maybe 50 blocks per month. We are unable to use a microwave processor. We process about 95% breast tissue. Someone mentioned the TP1020. Has anyone any comments on this processor. We are also looking to purchase a ph meter and a fume adsorber, any recommendations ? Your responses will be greatly appreciated as we are looking to purchase before the end of the month. Thanks for all your help and suggestions, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcruise@path.wustl.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SEsparza <@t> seton.org Thu Oct 7 11:36:55 2010 From: SEsparza <@t> seton.org (Esparza, Sandra) Date: Thu Oct 7 11:37:11 2010 Subject: [Histonet] problems with staining IHC In-Reply-To: <000701cb6637$aeccc180$6a03a8c0@apg> References: <000701cb6637$aeccc180$6a03a8c0@apg> Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB041DFD17@AUSEX2VS1.seton.org> If Erie is the manufacture of your Fisher slides then yes we have had the same problem with our IHC's. We use the Ventana Benchmark and have had this problem off and on for about 8 months. There seems to be no consistency with the quality of slides from Erie. We have just switched to the TruBond 200 slides which are distributed by STATLABS. These are working great for our IHC's. Sandra Sandra Esparza HT(ASCP)QIHC Lead Technologist Dell Children's Medical Center of Central Texas 512-324-0000 x87061 sesparza@seton.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Thursday, October 07, 2010 10:53 AM To: srishan@mail.holyname.org; histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] problems with staining IHC Hi Mala, We've used the Fisher superfrost plus slides for years and have never had that issue, it sounds more like you need to add some Tween to your buffer. That will help the solutions spread along your slide. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Thursday, October 07, 2010 9:32 AM To: histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Thu Oct 7 11:45:08 2010 From: plucas <@t> biopath.org (Paula Lucas) Date: Thu Oct 7 11:39:09 2010 Subject: [Histonet] Coverslip Film Message-ID: Hello Does anyone use a non-Sakura coverslip film on your Sakura coverslipper? My boss handed me a flyer from a company that sells film and it's a lot less money than the Sakura brand. Thanks, Paula Lab Manager Path Lab From kcruise <@t> path.wustl.edu Thu Oct 7 12:06:30 2010 From: kcruise <@t> path.wustl.edu (Cruise, Karen) Date: Thu Oct 7 12:06:50 2010 Subject: [Histonet] Responses Message-ID: <6428E3A48152704F838E99641B0973FA2EBF9B@PATHEXCH.wusm-path.wustl.edu> Thanks so much for your quick responses .Let me elaborate on my specific needs. We are a research lab that deals mainly with breast tissue. I have been led to believe that microwave processing may not be the way to go because of our specimens are being used for RNA and DNA studies. A quick turn around time is not a concern of ours since we process and hold specimens for future use based upon requests throughout the US. Our main concern is not purchasing more processor than what we need since we only process maybe 5-10 cassettes per week. Thanks again, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcruise@path.wustl.edu From michelecarr10 <@t> yahoo.com Thu Oct 7 12:15:50 2010 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Thu Oct 7 12:15:52 2010 Subject: [Histonet] p16 antibody Message-ID: <301798.64159.qm@web120720.mail.ne1.yahoo.com> Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw that MTM labs has it but it seems as though they sell it as a kit. Does anyone know where I could find this antibody that I could use on the bond autostaining machine. Thank you, Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Oct 7 12:28:14 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Oct 7 12:30:12 2010 Subject: [Histonet] p16 antibody In-Reply-To: <301798.64159.qm@web120720.mail.ne1.yahoo.com> References: <301798.64159.qm@web120720.mail.ne1.yahoo.com> Message-ID: If you want the IVD form of the antibody you have to go with MTM labs, they hold the patent. We buy the kit and take out the antibody and use the detection for research. It works very very well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Carr [michelecarr10@yahoo.com] Sent: Thursday, October 07, 2010 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw that MTM labs has it but it seems as though they sell it as a kit. Does anyone know where I could find this antibody that I could use on the bond autostaining machine. Thank you, Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Thu Oct 7 12:30:40 2010 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Thu Oct 7 12:30:50 2010 Subject: [Histonet] p16 antibody In-Reply-To: <301798.64159.qm@web120720.mail.ne1.yahoo.com> References: <301798.64159.qm@web120720.mail.ne1.yahoo.com> Message-ID: Hi Michele, Yes, it's true, only MTM labs sells it and they Sell it as a kit and they also sell it along with a negative control reagent. That's how I buy it. They have a "license" or something and no else can sell it now. Their cat# is 9518 and I think it's called the CINteck Histology V-kit, (you should double check with them.) They will make you "open up an account..." Good Luck Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Carr Sent: Thursday, October 07, 2010 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw that MTM labs has it but it seems as though they sell it as a kit. Does anyone know where I could find this antibody that I could use on the bond autostaining machine. Thank you, Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Thu Oct 7 12:32:11 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Thu Oct 7 12:32:17 2010 Subject: [Histonet] RE: Histonet Digest, Vol 83, Issue 8 Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C013753F3@mail.pcnm.com> Another consideration as a few have mentioned could be with your automated stainer (if you use one). We have a DAKO autostainer and we found our staining had become very sporadic. When I did a repeat run manually, everything was beautiful. We found that a pump on the stainer was going and it wasn't washing all the reagents off the slide between steps. In addition the probe needed to be replaced as it was drawing up air into the lines and the volumes it was dispensing on the slides was variable from slide to slide depending on how much air was in the probe lines at any given time. If you do use automation, when was the last time the machine had a PM? I agree with the others that tween in your wash buffer is a must. Joanne Clark, HT Pathology Consultants of New Mexico ---------------------------------------------------------------------- Message: 1 Date: Thu, 7 Oct 2010 07:32:13 -0700 From: srishan@mail.holyname.org Subject: [Histonet] problems with staining IHC To: histonet-bounces@lists.utsouthwestern.edu, Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 From kcruise <@t> path.wustl.edu Thu Oct 7 13:00:47 2010 From: kcruise <@t> path.wustl.edu (Cruise, Karen) Date: Thu Oct 7 13:00:54 2010 Subject: [Histonet] Equipment purchases responses Message-ID: <6428E3A48152704F838E99641B0973FA2EBF9D@PATHEXCH.wusm-path.wustl.edu> Thanks so much for your quick responses .Let me elaborate on my specific needs. We are a research lab that deals mainly with breast tissue. I have been led to believe that microwave processing may not be the way to go because of our specimens are being used for RNA and DNA studies. A quick turn around time is not a concern of ours since we process and hold specimens for future use based upon requests throughout the US. Our main concern is not purchasing more processor than what we need since we only process maybe 5-10 cassettes per week. Thanks again, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcruise@path.wustl.edu From rjbuesa <@t> yahoo.com Thu Oct 7 14:04:11 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 7 14:04:14 2010 Subject: [Histonet] Coverslip Film In-Reply-To: Message-ID: <230803.55111.qm@web65704.mail.ac4.yahoo.com> Once I tried one and it was harder, reacted less readily with xylene, and tended to peel-off easier. You could ask for a sample and try it. Ren? J. --- On Thu, 10/7/10, Paula Lucas wrote: From: Paula Lucas Subject: [Histonet] Coverslip Film To: histonet@lists.utsouthwestern.edu Date: Thursday, October 7, 2010, 12:45 PM Hello Does anyone use a non-Sakura coverslip film on your Sakura coverslipper?? My boss handed me a flyer from a company that sells film and it's a lot less money than the Sakura brand. Thanks, Paula Lab Manager Path Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vjp2105 <@t> columbia.edu Thu Oct 7 14:13:33 2010 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Thu Oct 7 14:13:42 2010 Subject: [Histonet] Bone IHC Message-ID: Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. From liz <@t> premierlab.com Thu Oct 7 14:19:38 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Oct 7 14:17:31 2010 Subject: [Histonet] ECM stain for growing cartilage Message-ID: Hello Everyone Is anyone out there aware of an ECM stain that can be used on growing tissue culture cells in a hydrogel. What I'm really looking for is a live stain I can use on the whole gel and see with a light microscope. I want to get a general idea of the amount of ECM that is growing. So it could just be a general bulk ECM (proteoglycan or collagen) stain that will show a contrast from the gel. And I want to be able to return the gel to the incubator and do the stain again multiple days later. I have searched extensively for something that would do this and I have come up with nothing. Any advice is appreciated and thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From liz <@t> premierlab.com Thu Oct 7 14:20:23 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Oct 7 14:18:28 2010 Subject: [Histonet] Bone IHC In-Reply-To: Message-ID: We lower the temp of retrieval to 70C for 2 hours and have good success with that. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa J. Phelan Sent: Thursday, October 07, 2010 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone IHC Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Oct 7 14:29:54 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 7 14:29:59 2010 Subject: [Histonet] Bone IHC In-Reply-To: Message-ID: <778481.86236.qm@web65712.mail.ac4.yahoo.com> If you have a bone that will require IHC, you will have to make sure that the Gross section is no more than 1.5 mm thick and?decalcify it in EDTA. The you have to make sure that the infiltration is complete. These are the 2 initial factors. Later you will have to section it as thin as you can, place the sections in (+) slides let them drain completely before going into the oven. Extra time in the oven is not really required if the aforementioned steps are done. Then add enough dishwasher soap to the HIER solution to get a solution of 2% and you will dewax and retrieve the antigen simultaneously. The sections will survive to complete the IHC procedure.Ren? J. --- On Thu, 10/7/10, Vanessa J. Phelan wrote: From: Vanessa J. Phelan Subject: [Histonet] Bone IHC To: "histonet@lists.utsouthwestern.edu" Date: Thursday, October 7, 2010, 3:13 PM Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval? (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anonwums1 <@t> gmail.com Thu Oct 7 15:08:29 2010 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Oct 7 15:08:36 2010 Subject: [Histonet] Bone IHC In-Reply-To: References: Message-ID: I've been experimenting with different ways to solve this problem myself. I fix my tissues in 10% zinc buffered formalin and decalcify in formic acid for 72 hours, followed by embedding and cutting 5 uM sections. >From trial and error, I've determined that incubating the slides flat on a slide warmer at 37C (or in my case, the bottom of an unused bacterial incubator) can prevent nearly all the detachment you observe but it's time dependent. I think the problem is that the sections get small amounts of water underneath them when you scoop them up off the water surface during sectioning and during HIER, that water boils and shears off the slide. If you leave the slides overnight, the slides were get destroyed during HIER. However, if you leave them for a week, they tend to be nearly untouched even at 95C for 10 mins. I'm currently in the process of determining if a few days is enough time. Adam On Thu, Oct 7, 2010 at 2:20 PM, Liz Chlipala wrote: > We lower the temp of retrieval to 70C for 2 hours and have good success > with that. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, Colorado 80308 > office (303) 682-3949 > fax (303) 682-9060 > www.premierlab.com > > > Ship to Address: > 1567 Skyway Drive, Unit E > Longmont, Colorado 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa > J. Phelan > Sent: Thursday, October 07, 2010 1:14 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone IHC > > Hi everyone, > > I was wondering if anyone has any tricks on how to get bone sections to > stop lifting off the slide through the IHC process? I leave them in the > oven for quite a while to make sure they are baked on, however after > antigen retrieval (pressure cooker for 20mins) most of the boney part > of the tissue comes off and the marrow and muscle stays put! The > sections are cut onto superfrost plus slides. > > Any help would be much appreciated, thanks. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From alisha <@t> ka-recruiting.com Thu Oct 7 16:12:10 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Oct 7 16:10:13 2010 Subject: [Histonet] Histology Supervisor Job in Texas! Message-ID: <1988412550.1286485930429.JavaMail.cfservice@SL4APP4> Hi Histonet Members I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working with a client in the Midland/Odessa Texas area to locate a Histology Supervisor. This 350+ bed community teaching hospital system has a very strong reputation in area. My client is looking for someone who is HT or HTL certified, has 5 + years for histology experience, and prefers someone with supervisory or lead experience. This hospital system offers a very competitive base salary/hourly rate, an outstanding benefits and retirement package, and relocation assistance. Please email me asap if you are interested in more details. Email alisha@ka-recruiting.com. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Laboratory Opportunities: Histotechnologist: 1. Histotech - Syracuse NY 2. Histology Manager - Michigan 3. Histotech - NYC 4. Histology Supervisor - GA 5. Histotech - NV (Must have Bachelors Degree) 6. Nashville, TN - Histotech 7. Histotech - Cape Cod, MA 8. Histology Supervisor - TX 9. General Manager of Anatomic Pathology - NJ If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From rsrichmond <@t> gmail.com Thu Oct 7 21:16:14 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Oct 7 21:16:19 2010 Subject: [Histonet] cassette labelers Message-ID: I'm advising a locum tenens client of mine about acquiring a cassette labeler. The only one I'm familiar with is Thermo Scientific's Cassette MicroWriter. Some questions for HistoNet: 1) A JCAHO inspector informed them that a cassette labeler will soon be required. Does anyone know if this is in fact the case? 2) Will the labeler print patients' names, or other second identifiers such as JCAHO now requires? 3) Are these labelers available in the used instrument market? 4) Is there any competition, or is the Thermo product all there is? 5) How well do these labelers withstand use? Bob Richmond Samurai Pathologist Knoxville TN From hlukey <@t> msn.com Thu Oct 7 21:41:34 2010 From: hlukey <@t> msn.com (Hugh Luk) Date: Thu Oct 7 21:41:39 2010 Subject: [Histonet] Equipment purchases responses Message-ID: Hi Karen, I am in a the same "Research" situation as you. If you are sure that all you will need is small (<100) tissue cassette processings, the Leica TP1020 should suffice. We call them "Dip-and-Dunks", as the tissue carousels from bucket to bucket. It is economical on reagents and is easy to use. Plan on 12+ hour processing runs for tissue with fat. Get the extra paraffin bucket (3) if you can. It is not a necessity for the routine processing run, but it is 'Handy' to have. However for fatty tissues like breast, the tissue processing is superior in vacuum infiltration units like the Leica ASP300S or a Tissue Tek VIP series. If you have the money, I recommend one of these. Both are work-horses and will give you practically no trouble. However, you will hear new terms like "Warm Water Flush", "Clean the Retort", and "Annual preventive maintenance." You will also triple your "Waste" reagents compared to the TP1020. I have a Leica ASP300. It was a refurbished unit, which I regret every so often, but it was necessary to procure due to money and time. Mostly money. It has saved my bu** more often than not. I can offer some recommendations for vendors like this if you wish. I also have access to the TP1020, ASP300S, Peloris, and the Tissue Teks 300, 5, and 6. I prefer the Tissue Tek 6, but all units have worked well for me. I have read other recommendations for the Leica Peloris or some variety of microwave tissue processor. These processors are absolutely fantastic and fast, but I have the same red-flags as you with RNA/DNA damage. No microwaves. The Peloris, in theory, will not cause further RNA/DNA damage, but it is advertised as "Faster Than Microwaves," so I would use caution pursuing this model until someone documents what it is doing to the nucleotides. Also, the Peloris is more expensive and it's 600 block capacity is too big for my (your?) needs. As for your portable fume hood and pH meter. pH meters range from cheap to super-expensive. The pocket version is cheap but falls into disrepair quickly. The bench-top models, requiring pH "Standard" calibrations and probe care, are great, but a pain to maintain and expensive to buy. You need to define what you need it for. Translational Pathology? Perhaps you can borrow? It seems to be a waste to have an expensive pH meter if (for example) you only measure buffers once a week. We have a Checkmate from Cardinal scientific, but I think this model has been discontinued. Fume hood; If you cannot get your facilities folks to put a real hood in, try Labconco or Thermo. Get one that can be used as a "Laminar Flow" (air filter parallel to desk), as formalin and xylene fumes lay close to the desk-top. There are lots of brands with different kinds and sizes of hoods. I believe that filtered hoods are not very efficient at cleaning the air of formalin (potassium permanganate filter) or xylene (carbon), simply because there is not enough air exchange. An example of our 36"x24"x30" self contained hood is in the link below: http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=82010-736&inE=1&highlight=82010-736 Hope this helps, Hugh Luk, HTL (ASCP) Pathology shared resource lab manager 1236 Lauhala street, Honolulu, HI 96813 > Date: Thu, 7 Oct 2010 13:00:47 -0500 > From: kcruise@path.wustl.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Equipment purchases responses > > Thanks so much for your quick responses .Let me elaborate on my specific needs. We are a research lab that deals mainly with breast tissue. I have been led to believe that microwave processing may not be the way to go because of our specimens are being used for RNA and DNA studies. A quick turn around time is not a concern of ours since we process and hold specimens for future use based upon requests throughout the US. Our main concern is not purchasing more processor than what we need since we only process maybe 5-10 cassettes per week. > > Thanks again, > Karen > > > > > > Karen E. Cruise > Histologist / Research Technician II > Washington University School of Medicine > Laboratory for Translational Pathology > 216 S. Kingshighway Rm #2332 > St Louis, MO 63110 > 314-454-8636 Office > 314-454-5525 Fax > kcruise@path.wustl.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Kuhnla <@t> chsli.org Fri Oct 8 05:38:28 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri Oct 8 05:39:29 2010 Subject: [Histonet] Bone IHC In-Reply-To: References: Message-ID: Are you placing this bone section on a pre-cut control slides? The section will adhere better on its own slide. Are you drying your slides at room temp prior to baking? It always helps to remove all trapped water from under the section prior to baking. Post fixing with formalin fumes is said to help. Remove slides from oven. Place in a rack in a staining cup w formalin just covering the bottom of the cup (not covering the section). Cover and allow the fumes to 'post fix' for 15 minutes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa J. Phelan Sent: Thursday, October 07, 2010 3:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone IHC Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From Farnana <@t> nehealth.com Fri Oct 8 07:14:40 2010 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Fri Oct 8 07:14:51 2010 Subject: [Histonet] mislabeling of slides and blocks Message-ID: <4CAED2F0.26ED.00D9.1@nehealth.com> Hello everyone, I was wondering if you could share your policy for employee infractions of mislabeling blocks and slides? I would like to get an idea of what the standard is out there. Any help would be appreciated. Thank you Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From christen <@t> vet.k-state.edu Fri Oct 8 08:05:12 2010 From: christen <@t> vet.k-state.edu (Shelly Christenson) Date: Fri Oct 8 08:05:17 2010 Subject: [Histonet] cassette labelers In-Reply-To: References: Message-ID: <06E342B6098ED9478347E1407764C80402807453@VETMXHT.ads.vet.k-state.edu> Surgipath/Leica Biosystems has a cassette printer you may want to look at. You can set it up to type a double line on the cassette. We have two of them and have had no problems with them. Shelly Christenson HT(ASCP) Veterinary Diagnostic Labortory Kansas State University Mosier Hall L-216 Manhattan, Kansas 66506 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Robert Richmond [rsrichmond@gmail.com] Sent: Thursday, October 07, 2010 9:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labelers I'm advising a locum tenens client of mine about acquiring a cassette labeler. The only one I'm familiar with is Thermo Scientific's Cassette MicroWriter. Some questions for HistoNet: 1) A JCAHO inspector informed them that a cassette labeler will soon be required. Does anyone know if this is in fact the case? 2) Will the labeler print patients' names, or other second identifiers such as JCAHO now requires? 3) Are these labelers available in the used instrument market? 4) Is there any competition, or is the Thermo product all there is? 5) How well do these labelers withstand use? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Oct 8 08:23:06 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Oct 8 08:23:11 2010 Subject: [Histonet] cassette labelers In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640398777DA2@CHEXCMS10.one.ads.che.org> I haven't heard anything about the requirement. Leica and Sakura sell the exact same instrument, so both are excellent. They can be interfaced with LIS and barcoded, etc. We've had the Sakura cassette and slide printers for 6 or 7 years. TBS has good instruments also. Not sure about how cassettes print, but they are very legible and can include the name. Slides are etched and are very neat and readable. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, October 07, 2010 22:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labelers I'm advising a locum tenens client of mine about acquiring a cassette labeler. The only one I'm familiar with is Thermo Scientific's Cassette MicroWriter. Some questions for HistoNet: 1) A JCAHO inspector informed them that a cassette labeler will soon be required. Does anyone know if this is in fact the case? 2) Will the labeler print patients' names, or other second identifiers such as JCAHO now requires? 3) Are these labelers available in the used instrument market? 4) Is there any competition, or is the Thermo product all there is? 5) How well do these labelers withstand use? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Wanda.Smith <@t> HCAhealthcare.com Fri Oct 8 09:05:07 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Oct 8 09:05:15 2010 Subject: [Histonet] cassette labelers In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1394CA2C3C@NADCWPMSGCMS03.hca.corpad.net> We have a new Leica/Surgipath cassette labeler after using the old Surgipath cassette labeler for approximately 10 years with no problems. Our Biomed would change the print head ever year or two because the pins would wear our. The new Leica/Surgipath labeler prints accession number and patient's name. You print cassettes one at a time and you can set the labeler to automatically advance the case number or block number. It's fast and easy! We love it!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, October 07, 2010 10:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labelers I'm advising a locum tenens client of mine about acquiring a cassette labeler. The only one I'm familiar with is Thermo Scientific's Cassette MicroWriter. Some questions for HistoNet: 1) A JCAHO inspector informed them that a cassette labeler will soon be required. Does anyone know if this is in fact the case? 2) Will the labeler print patients' names, or other second identifiers such as JCAHO now requires? 3) Are these labelers available in the used instrument market? 4) Is there any competition, or is the Thermo product all there is? 5) How well do these labelers withstand use? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Oct 8 10:41:04 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Oct 8 10:41:13 2010 Subject: [Histonet] antibody GCET2 Message-ID: Hi! Has anybody experiences with this antibody for IHC on human FFPET? I found only Sigma-Aldrich as supplier and their website doesn't show a datasheet with recommended titer. I'm thankful for any information. Gudrun Lang From KMB01 <@t> grh.org Fri Oct 8 10:45:11 2010 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Fri Oct 8 10:45:15 2010 Subject: [Histonet] THANKS Message-ID: Thank you to all who answered about Helicobacter stains. I knew I could count on the HistoNet to help. Have a great week end. Kathy Gorham GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From crochieresteve <@t> aol.com Fri Oct 8 10:54:08 2010 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Fri Oct 8 10:54:32 2010 Subject: [Histonet] To histonet@lists.utsouthwestern.edu! Message-ID: <8CD35182D6DD475-C5C-851B@webmail-d047.sysops.aol.com> hi! histonet@lists.utsouthwestern.edu!http://www.paolaguerriero.it/to.php Best regards, crochieresteve@aol.com From Janice.Mahoney <@t> alegent.org Fri Oct 8 11:40:37 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Oct 8 11:42:48 2010 Subject: [Histonet] mislabeling of slides and blocks In-Reply-To: <4CAED2F0.26ED.00D9.1@nehealth.com> References: <4CAED2F0.26ED.00D9.1@nehealth.com> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CD99928@EXCHMBC2.ad.ah.local> I'd be happy to share mine but it is very strict. People are given a final written warning for the first mislabel not caught in the department and can be fired for the second within a year. I highly suggest the Ventana Vantage (there are others but in my opinion Vantage is the best) bar code system for patient safety. It also relieves the stress on your Histo techs because the bar coding helps them assure they have the correct slide, block, etc. Techs are hard to find and if we start firing for inevitable human error we will cause even more tech shortages. When you look at all the costs that a mislabel can incur, (law suits, replacing personnel, rework, etc.) the cost of the software is not too bad. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Farnan [Farnana@nehealth.com] Sent: Friday, October 08, 2010 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mislabeling of slides and blocks Hello everyone, I was wondering if you could share your policy for employee infractions of mislabeling blocks and slides? I would like to get an idea of what the standard is out there. Any help would be appreciated. Thank you Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From kreaser <@t> vet.upenn.edu Fri Oct 8 11:58:03 2010 From: kreaser <@t> vet.upenn.edu (Karie Reaser) Date: Fri Oct 8 11:58:06 2010 Subject: [Histonet] GLP Compliant Laboratory Message-ID: <528881174.1967071286557083477.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Hi, I was wondering if anyone runs a GLP Compliant laboratory and is willing to advise. Thanks Have a great day all you Histonetters! -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:kreaser@vet.upenn.edu From MadaryJ <@t> MedImmune.com Fri Oct 8 12:19:54 2010 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Oct 8 12:20:00 2010 Subject: [Histonet] glp lab Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A130287C660@MD1EV002.medimmune.com> We run one here and trust me when I tell you the best thing to do is to actually go to one and see for yourself what the deal is. There is a lot to it. I suggest you take some classes as well in GxP. Imagine this, an FDA inspector comes in to the lab and picks out one HE slide. That slide has several SOP's attached to it and those SOP need to be in NAACLS format, the folks who did all the work on the HE from necropsy through labeling need to have a cv on file showing relevant training and experience that states they are trained to do whatever their part of the HE they did, and that they read the SOP used on the process. Which hurts people like Joe Nocito who still can't read(hi Joe). If they cut the section there needs to be proof they are trained to section. Then oh yeah all of the reagents used to perform necropsy, trimming, embedding, microtomy, staining, coverlsipping, labeling, archiving(another GLP situation) need to be dated and labeled with lot numbers, batch numbers. There needs to be logsheets on everything from temps of the water bath temps and water checks, when the slides were stained, when the tissue was run on the processor and all of the maintenance as well as proper use of maintenance SOP's. Did you use a scale or pipette? Where are the calibration logs? When you calibrated the Ph meter did you use outdated ph buffers, I hope not loser cuz you just failed that part of the inspection. Take any CAP inspection and multiply it 5 fold on the level of detail on everything. Running a GLP lab is doable and a great way to run a lab, but you will need to increase your staff in the lab just to cover the documentation. Good Luck. This is as far as we can go here due to the nature of our business. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From sgoebel <@t> xbiotech.com Fri Oct 8 12:41:43 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 8 12:41:49 2010 Subject: [Histonet] mislabeling of slides and blocks Message-ID: <20101008104143.9e2d9aa830e8449a2412eb1e4f2f067e.b9f20bea0d.wbe@email04.secureserver.net> Holy cow man!! Fired for mislabeling 2 times in 260 days!!& Have you never been human and made a mistake?! I could understa if it was a tech on a regular basis messing up, but that seems a little WOW!! Just my opinion... Sarah Goebel, B.A., HT (ASCP) < Histo XBiotech USA Inc. 8201 East Rivers Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] mislabeling of slides and blocks From: "Mahoney,Janice A" <[1] Date: Fri, October 08, 2010 9:40 am To: Amy Farnan <[2]Farnana@nehealth "[3]histonet@lists.utsout histonet@lists.utsouthwestern.edu> Cc: "Dean, Sherry" <[5]she I'd be happy to share mine but it is very strict. People are given a final department and ca I highly suggest the Ventana Vantage (there are others but in my opinion Va also relieves th helps them assure they hard to find and if we start will cause even more tech shortages.< costs that a mislabel can incur, (law suits, repla rework, etc.) the cost of the software is not too bad. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: [6]histonet [[7]histonet-bounces@lists.utsouthwestern.edu] On Farnan [[8]Farnana@nehea Sent: Friday, October 08, 2010 7:14 AM To: [9]histonet@lists.uts Subject: [Histonet] mislabeling of slides and blocks Hello everyone, I was wondering if you could share your policy for employee infractions of idea of what the stan appreciated. Thank you Disclaimer: The information in this message is confidential. If you are no this message _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouth [11]http: Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is fa quality care The information contained in this communication, including attachments, is use of the addressees. copying is strictly prohibite this communication in error, please delivery by return e-mail message from your com although all attachments have been scanned at the sou viruses, the recipient should check any attachments for the presenc of viruses before opening. Alegent Health accepts no liability for any d your c _______________________________________________ Histonet mailing list [12]Histonet@lists.utsouth [13]http: References 1. 3D"mailto:Janice.Mahoney@alegent.org" 2. 3D"mailto:Farnana@nehealth.com" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet@lists.utsouthwestern.edu" 5. 3D"mailto:sherry.dean@ventana.roche.com" 6. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 7. 3D"mailto:histonet-bounces@l 8. 3D"mailto:Farnana@nehealth.com" 9. 3D"mailto:histonet@lists.utsouthwestern.edu" 10. 3D"mailto:Histonet@lists.utsouthwestern.edu" 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 12. 3D"mailto:Histonet@lists.utsouthwestern.edu" 13. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From sgoebel <@t> xbiotech.com Fri Oct 8 12:42:02 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 8 12:42:06 2010 Subject: [Histonet] GLP Compliant Laboratory Message-ID: <20101008104202.9e2d9aa830e8449a2412eb1e4f2f067e.b33a34a24b.wbe@email04.secureserver.net> What are you needing? Sarah Goebel, B.A., HT (ASCP) XBiotech USA Inc. 8201 East R Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] GLP Compliant Laboratory From: Karie Reaser <[1]kreaser@vet Date: Fri, October 08, 2010 9:58 am To: [2]Histonet@lists.uts Hi, I was wondering if anyone runs a GLP Compliant laboratory and is willing to -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:[3]kreaser@vet.upenn.edu _______________________________________________ Histonet mailing list [4]Histonet@lists.utsouth [5]http: References 1. 3D"mailto:kreaser@vet.upenn.edu" 2. 3D"mailto:Histonet@lists.utsouthwestern.edu" 3. 3D"mailto:kreaser@vet.upenn.edu" 4. 3D"mailto:Histonet@lists.utsouthwestern.edu" 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Farnana <@t> nehealth.com Fri Oct 8 12:52:33 2010 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Fri Oct 8 12:52:39 2010 Subject: [Histonet] mislabeling of slides and blocks In-Reply-To: <20101008104143.9e2d9aa830e8449a2412eb1e4f2f067e.b9f20bea0d.wbe@email04.secureserver.net> References: <20101008104143.9e2d9aa830e8449a2412eb1e4f2f067e.b9f20bea0d.wbe@email04.secureserver.net> Message-ID: <4CAF2221.26ED.00D9.1@nehealth.com> Sarah, What is your policy? Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From mucram11 <@t> comcast.net Fri Oct 8 13:02:15 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Oct 8 13:02:19 2010 Subject: [Histonet] mislabeling of slides and blocks In-Reply-To: <20101008104143.9e2d9aa830e8449a2412eb1e4f2f067e.b9f20bea0d.wbe@email04.secureserver.net> Message-ID: <588443311.1143.1286560935310.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I agree with Janice.? Except we need three incidents and the person is fired on the third.??If it is a pathologist or the case is called negative when it was not and the patient goes untreated we are in a legal battle.? We also have pathologist and doctors who can make it happen faster if it is one of their cases that is called wrong.? We can't have patient care and diagnosis compromised because someone was not paying attention.? Yes, I have bad days, too.??If I am having one I?have someone else check me if there is any quesiton.? ? Pam Marcum UAMS AP Manager ----- Original Message ----- From: sgoebel@xbiotech.com To: "Janice A Mahoney" Cc: histonet@lists.utsouthwestern.edu, "Sherry Dean" , "Amy Farnan" Sent: Friday, October 8, 2010 12:41:43 PM Subject: RE: [Histonet] mislabeling of slides and blocks ?? Holy ?cow ?man!! ? Fired for mislabeling 2 times in 260 days!!&= nbsp; ?? Have ?you ?never been human and made a mistake?! ?I could understa= nd ?? if ?it ?was ?a ?tech ?on ?a regular basis messing up, but that seems a ?? little = crazy, especially if you are in a high volume hospital!! ?? WOW!! ?? Just my opinion... ?? Sarah Goebel, B.A., HT (ASCP) ?? <= div> ?? Histo= technician ?? XBiotech USA Inc. ?? 8201 East Rivers= ide Dr. Bldg 4 Suite 100 ?? Austin, Texas ?78744 ?? (512)386-5107 ?? -------- Original Message -------- ?? Subject: RE: [Histonet] mislabeling of slides and blocks ?? From: "Mahoney,Janice A" <[1]= Janice.Mahoney@alegent.org> ?? Date: Fri, October 08, 2010 9:40 am ?? To: Amy Farnan <[2]Farnana@nehealth= .com>, ?? "[3]histonet@lists.utsout= ? ? ? ? ? ? hwestern.edu" ? ? ? ? ? ? <[4] ? histonet@lists.utsouthwestern.edu> ?? Cc: "Dean, Sherry" <[5]she= rry.dean@ventana.roche.com> ?? I'd ?be ?happy to share mine but it is very strict. People are given a ?? final= ?written ?warning ?for ?the ?first ?mislabel ?not caught in the ?? department and ca= n be fired for the second within a year. ?? I ?highly ?suggest ?the ?Ventana ?Vantage ?(there are others but in my ?? opinion ?Va= ntage is the best) bar code system for patient safety. It ?? also ?relieves th= e stress on your Histo techs because the bar coding ?? helps them assure they = have the correct slide, block, etc. Techs are ?? hard ?to ?find ?and ?if we start= firing for inevitable human error we ?? will ?cause ?even ?more tech shortages.<= br> When you look at all the ?? costs ?that ?a ?mislabel can incur, (law suits, repla= cing personnel, ?? rework, etc.) the cost of the software is not too bad. ?? Janice Mahoney HT(ASCP) ?? Histology/Cytology Coordinator ?? Alegent Health Laboratory ?? 4955 F Street ?? Omaha, NE ?? (402)717-2889 ?? fax(402)717-5231 ?? ________________________________________ ?? From: ? ? ? ? ?[6]histonet= ? ? ? ? ?-bounces@lists.utsouthwestern.edu ?? [[7]histonet-bounces@lists.utsouthwestern.edu] ?On ?= ?Behalf ?Of ?Amy ?? Farnan [[8]Farnana@nehea= lth.com] ?? Sent: Friday, October 08, 2010 7:14 AM ?? To: [9]histonet@lists.uts= outhwestern.edu ?? Subject: [Histonet] mislabeling of slides and blocks ?? Hello everyone, ?? I ? was ?wondering ?if ?you ?could ?share ?your ?policy ?for ?employee ?? infractions of = mislabeling blocks and slides? I would like to get an ?? idea ?of ?what ?the ?stan= ?dard ?is ?out ?there. ?Any ?help ?would be ?? appreciated. ?? Thank you ?? Disclaimer: ?The ?information ?in this message is confidential. If you ?? are no= t the intended recipient, do not disclose, copy, or distribute ?? this message= , and please immediately contact the sender. ?? _______________________________________________ ?? Histonet mailing list ?? [10]Histonet@lists.utsouth= western.edu ?? [11]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet ?? Sponsored ?by Catholic Health Initiatives and Immanuel, Alegent Health ?? is ?fa= ithful to the healing ministry of Jesus Christ, providing high ?? quality care= for the body, mind and spirit of every person. ?? The ? information ? contained ? in ? this ? communication, ? including ?? attachments, ?is ?= confidential and private and intended only for the ?? use of the addressees. = Unauthorized use, disclosure, distribution or ?? copying ?is strictly prohibite= d and may be unlawful. If you received ?? this ?communication ?in ?error, ?please= ?inform ?us ?of the erroneous ?? delivery ?by return e-mail message from your com= puter. Additionally, ?? although ?all ?attachments ?have ?been ?scanned ?at ?the ?sou= rce for ?? viruses, the recipient should check any attachments for the presenc= e ?? of viruses before opening. Alegent Health accepts no liability for any ?? d= amage caused by any virus transmitted by this e-mail. Thank you for ?? your c= ooperation. ?? _______________________________________________ ?? Histonet mailing list ?? [12]Histonet@lists.utsouth= western.edu ?? [13]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References ?? 1. 3D"mailto:Janice.Mahoney@alegent.org" ?? 2. 3D"mailto:Farnana@nehealth.com" ?? 3. 3D"mailto:histonet@lists.utsouthwestern.edu" ?? 4. 3D"mailto:histonet@lists.utsouthwestern.edu" ?? 5. 3D"mailto:sherry.dean@ventana.roche.com" ?? 6. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" ?? 7. 3D"mailto:histonet-bounces@l ? 8. 3D"mailto:Farnana@nehealth.com" ?? 9. 3D"mailto:histonet@lists.utsouthwestern.edu" ??10. 3D"mailto:Histonet@lists.utsouthwestern.edu" ??11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ??12. 3D"mailto:Histonet@lists.utsouthwestern.edu" ??13. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: sgoebel@xbiotech.com To: "Janice A Mahoney" Cc: histonet@lists.utsouthwestern.edu, "Sherry Dean" , "Amy Farnan" Sent: Friday, October 8, 2010 12:41:43 PM Subject: RE: [Histonet] mislabeling of slides and blocks ?? Holy ?cow ?man!! ? Fired for mislabeling 2 times in 260 days!!&= nbsp; ?? Have ?you ?never been human and made a mistake?! ?I could understa= nd ?? if ?it ?was ?a ?tech ?on ?a regular basis messing up, but that seems a ?? little = crazy, especially if you are in a high volume hospital!! ?? WOW!! ?? Just my opinion... ?? Sarah Goebel, B.A., HT (ASCP) ?? <= div> ?? Histo= technician ?? XBiotech USA Inc. ?? 8201 East Rivers= ide Dr. Bldg 4 Suite 100 ?? Austin, Texas ?78744 ?? (512)386-5107 ?? -------- Original Message -------- ?? Subject: RE: [Histonet] mislabeling of slides and blocks ?? From: "Mahoney,Janice A" <[1]= Janice.Mahoney@alegent.org> ?? Date: Fri, October 08, 2010 9:40 am ?? To: Amy Farnan <[2]Farnana@nehealth= .com>, ?? "[3]histonet@lists.utsout= ? ? ? ? ? ? hwestern.edu" ? ? ? ? ? ? <[4] ? histonet@lists.utsouthwestern.edu> ?? Cc: "Dean, Sherry" <[5]she= rry.dean@ventana.roche.com> ?? I'd ?be ?happy to share mine but it is very strict. People are given a ?? final= ?written ?warning ?for ?the ?first ?mislabel ?not caught in the ?? department and ca= n be fired for the second within a year. ?? I ?highly ?suggest ?the ?Ventana ?Vantage ?(there are others but in my ?? opinion ?Va= ntage is the best) bar code system for patient safety. It ?? also ?relieves th= e stress on your Histo techs because the bar coding ?? helps them assure they = have the correct slide, block, etc. Techs are ?? hard ?to ?find ?and ?if we start= firing for inevitable human error we ?? will ?cause ?even ?more tech shortages.<= br> When you look at all the ?? costs ?that ?a ?mislabel can incur, (law suits, repla= cing personnel, ?? rework, etc.) the cost of the software is not too bad. ?? Janice Mahoney HT(ASCP) ?? Histology/Cytology Coordinator ?? Alegent Health Laboratory ?? 4955 F Street ?? Omaha, NE ?? (402)717-2889 ?? fax(402)717-5231 ?? ________________________________________ ?? From: ? ? ? ? ?[6]histonet= ? ? ? ? ?-bounces@lists.utsouthwestern.edu ?? [[7]histonet-bounces@lists.utsouthwestern.edu] ?On ?= ?Behalf ?Of ?Amy ?? Farnan [[8]Farnana@nehea= lth.com] ?? Sent: Friday, October 08, 2010 7:14 AM ?? To: [9]histonet@lists.uts= outhwestern.edu ?? Subject: [Histonet] mislabeling of slides and blocks ?? Hello everyone, ?? I ? was ?wondering ?if ?you ?could ?share ?your ?policy ?for ?employee ?? infractions of = mislabeling blocks and slides? I would like to get an ?? idea ?of ?what ?the ?stan= ?dard ?is ?out ?there. ?Any ?help ?would be ?? appreciated. ?? Thank you ?? Disclaimer: ?The ?information ?in this message is confidential. If you ?? are no= t the intended recipient, do not disclose, copy, or distribute ?? this message= , and please immediately contact the sender. ?? _______________________________________________ ?? Histonet mailing list ?? [10]Histonet@lists.utsouth= western.edu ?? [11]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet ?? Sponsored ?by Catholic Health Initiatives and Immanuel, Alegent Health ?? is ?fa= ithful to the healing ministry of Jesus Christ, providing high ?? quality care= for the body, mind and spirit of every person. ?? The ? information ? contained ? in ? this ? communication, ? including ?? attachments, ?is ?= confidential and private and intended only for the ?? use of the addressees. = Unauthorized use, disclosure, distribution or ?? copying ?is strictly prohibite= d and may be unlawful. If you received ?? this ?communication ?in ?error, ?please= ?inform ?us ?of the erroneous ?? delivery ?by return e-mail message from your com= puter. Additionally, ?? although ?all ?attachments ?have ?been ?scanned ?at ?the ?sou= rce for ?? viruses, the recipient should check any attachments for the presenc= e ?? of viruses before opening. Alegent Health accepts no liability for any ?? d= amage caused by any virus transmitted by this e-mail. Thank you for ?? your c= ooperation. ?? _______________________________________________ ?? Histonet mailing list ?? [12]Histonet@lists.utsouth= western.edu ?? [13]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References ?? 1. 3D"mailto:Janice.Mahoney@alegent.org" ?? 2. 3D"mailto:Farnana@nehealth.com" ?? 3. 3D"mailto:histonet@lists.utsouthwestern.edu" ?? 4. 3D"mailto:histonet@lists.utsouthwestern.edu" ?? 5. 3D"mailto:sherry.dean@ventana.roche.com" ?? 6. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" ?? 7. 3D"mailto:histonet-bounces@l ? 8. 3D"mailto:Farnana@nehealth.com" ?? 9. 3D"mailto:histonet@lists.utsouthwestern.edu" ??10. 3D"mailto:Histonet@lists.utsouthwestern.edu" ??11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ??12. 3D"mailto:Histonet@lists.utsouthwestern.edu" ??13. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Fri Oct 8 13:08:14 2010 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Oct 8 13:08:17 2010 Subject: [Histonet] mislabeling of slides and blocks In-Reply-To: <4CAED2F0.26ED.00D9.1@nehealth.com> References: <4CAED2F0.26ED.00D9.1@nehealth.com> Message-ID: Our policy deals with general "serious infractions" which are any error which could potentially harm the patient, 2 examples are embedding floaters and slide mislabeling. We use bar-coding for our blocks and slides as well as a rather sophisticated in-house-made tracking software. The way it works here, you scan a block and the label prints out telling you what is ordered and needs to be cut. We implemented a strict policy of making sure you match the block profile with the tissue profile on the slide a second time before it leaves the cutting bench. The first mistake is a meeting. Second mistake is a verbal warning. Third and fourth mistake is a written warning, and the final one is termination. With over 100,000 blocks cut and well over 150,000 slides and 20 employees, since we established this policy, I have not had two mistakes by the same person. This policy as well as the fantastic hard work by our employees has been quite successful. Good luck. On Fri, Oct 8, 2010 at 5:14 AM, Amy Farnan wrote: > Hello everyone, > > I was wondering if you could share your policy for employee infractions of > mislabeling blocks and slides? I would like to get an idea of what the > standard is out there. Any help would be appreciated. > > Thank you > > Disclaimer: The information in this message is confidential. If you are > not the intended recipient, do not disclose, copy, or distribute this > message, and please immediately contact the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From higginst <@t> amapath.com Fri Oct 8 13:10:27 2010 From: higginst <@t> amapath.com (Tim Higgins) Date: Fri Oct 8 13:15:09 2010 Subject: [Histonet] mislabeling of slides and blocks In-Reply-To: <20101008104143.9e2d9aa830e8449a2412eb1e4f2f067e.b9f20bea0d.wbe@email04.secureserver.net> References: <20101008104143.9e2d9aa830e8449a2412eb1e4f2f067e.b9f20bea0d.wbe@email04.secureserver.net> Message-ID: <000001cb6714$17a83ea0$6a03a8c0@apg> Glad I never worked at that lab, never would have made to a supervisory role. I have to agree with Sarah, two infractions in a 12 month period is extremely severe. I don't care if you have Vantage or any one of the million tracking software packages out there. Especially with the shortage of qualified Histotechs out there to be so picky is not good. Anyone that has worked for a busy Histology lab knows mistakes happen, we all want them caught prior to going out but there are so many areas that these mistakes can happen. We do have a policy on mislabeled slides (or any infraction), there is a verbal warning, written warning, final warning and then if it happens again we would go to more of an extreme conclusion. That being said, we also would go over with them how the mistake happened, ways to avoid that type of mistake and then track that employee's slides more closely. Now if an employee made the first mistake in January and the 4th one in December of that same year I am still not sure we would dismiss that person. Especially if than employee in good standing, maybe there are extenuating circumstances going on. Two and out is pretty severe. Just my two cents! Thanks, Timothy Higgins, HT(ASCP) QIHC Histology Manager APA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Friday, October 08, 2010 12:42 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Dean, Sherry; Amy Farnan Subject: RE: [Histonet] mislabeling of slides and blocks Holy cow man!! Fired for mislabeling 2 times in 260 days!!&=bsp; Have you never been human and made a mistake?! I could understa=d if it was a tech on a regular basis messing up, but that seems a little =razy, especially if you are in a high volume hospital!! WOW!! Just my opinion... Sarah Goebel, B.A., HT (ASCP) <=iv> Histo=echnician XBiotech USA Inc. 8201 East Rivers=de Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] mislabeling of slides and blocks From: "Mahoney,Janice A" <[1]=anice.Mahoney@alegent.org> Date: Fri, October 08, 2010 9:40 am To: Amy Farnan <[2]Farnana@nehealth=com>, "[3]histonet@lists.utsout= hwestern.edu" <[4] histonet@lists.utsouthwestern.edu> Cc: "Dean, Sherry" <[5]she=ry.dean@ventana.roche.com> I'd be happy to share mine but it is very strict. People are given a final=written warning for the first mislabel not caught in the department and ca= be fired for the second within a year. I highly suggest the Ventana Vantage (there are others but in my opinion Va=tage is the best) bar code system for patient safety. It also relieves th= stress on your Histo techs because the bar coding helps them assure they =ave the correct slide, block, etc. Techs are hard to find and if we start=iring for inevitable human error we will cause even more tech shortages.<=r> When you look at all the costs that a mislabel can incur, (law suits, repla=ing personnel, rework, etc.) the cost of the software is not too bad. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: [6]histonet= -bounces@lists.utsouthwestern.edu [[7]histonet-bounces@lists.utsouthwestern.edu] On =Behalf Of Amy Farnan [[8]Farnana@nehea=th.com] Sent: Friday, October 08, 2010 7:14 AM To: [9]histonet@lists.uts=uthwestern.edu Subject: [Histonet] mislabeling of slides and blocks Hello everyone, I was wondering if you could share your policy for employee infractions of =islabeling blocks and slides? I would like to get an idea of what the stan=dard is out there. Any help would be appreciated. Thank you Disclaimer: The information in this message is confidential. If you are no= the intended recipient, do not disclose, copy, or distribute this message= and please immediately contact the sender. _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouth=estern.edu [11]http:=/lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is fa=thful to the healing ministry of Jesus Christ, providing high quality care=or the body, mind and spirit of every person. The information contained in this communication, including attachments, is =onfidential and private and intended only for the use of the addressees. =nauthorized use, disclosure, distribution or copying is strictly prohibite= and may be unlawful. If you received this communication in error, please=inform us of the erroneous delivery by return e-mail message from your com=uter. Additionally, although all attachments have been scanned at the sou=ce for viruses, the recipient should check any attachments for the presenc= of viruses before opening. Alegent Health accepts no liability for any d=mage caused by any virus transmitted by this e-mail. Thank you for your c=operation. _______________________________________________ Histonet mailing list [12]Histonet@lists.utsouth=estern.edu [13]http:=/lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:Janice.Mahoney@alegent.org" 2. 3D"mailto:Farnana@nehealth.com" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet@lists.utsouthwestern.edu" 5. 3D"mailto:sherry.dean@ventana.roche.com" 6. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 7. 3D"mailto:histonet-bounces@l 8. 3D"mailto:Farnana@nehealth.com" 9. 3D"mailto:histonet@lists.utsouthwestern.edu" 10. 3D"mailto:Histonet@lists.utsouthwestern.edu" 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 12. 3D"mailto:Histonet@lists.utsouthwestern.edu" 13. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Fri Oct 8 13:31:45 2010 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Oct 8 13:32:38 2010 Subject: [Histonet] mislabeling of slides and blocks In-Reply-To: <000001cb6714$17a83ea0$6a03a8c0@apg> References: <20101008104143.9e2d9aa830e8449a2412eb1e4f2f067e.b9f20bea0d.wbe@email04.secureserver.net> <000001cb6714$17a83ea0$6a03a8c0@apg> Message-ID: <00e301cb6717$1c163fc0$5442bf40$@com> Tim, I would both agree and disagree with your statement. We're all human. We all make mistakes. However, I do believe that 'systems' can be put in place which can render the likelihood of errors to almost zero. By 'systems', I mean either computer systems or some manual mechanism that may require checking and rechecking, perhaps by different parties. While I'm not an expert on other people's tracking systems, I do believe I know my own system pretty well, and I would tell you that it would be very, very difficult, if not impossible to mislabel blocks and slides in our system. Could you do it? Yes, I can imagine anyone can defeat a computerized system or a manual system if that is their intent, but then that's another question. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Friday, October 08, 2010 1:10 PM To: sgoebel@xbiotech.com; 'Mahoney,Janice A' Cc: histonet@lists.utsouthwestern.edu; 'Dean, Sherry'; 'Amy Farnan' Subject: RE: [Histonet] mislabeling of slides and blocks Glad I never worked at that lab, never would have made to a supervisory role. I have to agree with Sarah, two infractions in a 12 month period is extremely severe. I don't care if you have Vantage or any one of the million tracking software packages out there. Especially with the shortage of qualified Histotechs out there to be so picky is not good. Anyone that has worked for a busy Histology lab knows mistakes happen, we all want them caught prior to going out but there are so many areas that these mistakes can happen. We do have a policy on mislabeled slides (or any infraction), there is a verbal warning, written warning, final warning and then if it happens again we would go to more of an extreme conclusion. That being said, we also would go over with them how the mistake happened, ways to avoid that type of mistake and then track that employee's slides more closely. Now if an employee made the first mistake in January and the 4th one in December of that same year I am still not sure we would dismiss that person. Especially if than employee in good standing, maybe there are extenuating circumstances going on. Two and out is pretty severe. Just my two cents! Thanks, Timothy Higgins, HT(ASCP) QIHC Histology Manager APA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@xbiotech.com Sent: Friday, October 08, 2010 12:42 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Dean, Sherry; Amy Farnan Subject: RE: [Histonet] mislabeling of slides and blocks Holy cow man!! Fired for mislabeling 2 times in 260 days!!&=bsp; Have you never been human and made a mistake?! I could understa=d if it was a tech on a regular basis messing up, but that seems a little =razy, especially if you are in a high volume hospital!! WOW!! Just my opinion... Sarah Goebel, B.A., HT (ASCP) <=iv> Histo=echnician XBiotech USA Inc. 8201 East Rivers=de Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] mislabeling of slides and blocks From: "Mahoney,Janice A" <[1]=anice.Mahoney@alegent.org> Date: Fri, October 08, 2010 9:40 am To: Amy Farnan <[2]Farnana@nehealth=com>, "[3]histonet@lists.utsout= hwestern.edu" <[4] histonet@lists.utsouthwestern.edu> Cc: "Dean, Sherry" <[5]she=ry.dean@ventana.roche.com> I'd be happy to share mine but it is very strict. People are given a final=written warning for the first mislabel not caught in the department and ca= be fired for the second within a year. I highly suggest the Ventana Vantage (there are others but in my opinion Va=tage is the best) bar code system for patient safety. It also relieves th= stress on your Histo techs because the bar coding helps them assure they =ave the correct slide, block, etc. Techs are hard to find and if we start=iring for inevitable human error we will cause even more tech shortages.<=r> When you look at all the costs that a mislabel can incur, (law suits, repla=ing personnel, rework, etc.) the cost of the software is not too bad. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: [6]histonet= -bounces@lists.utsouthwestern.edu [[7]histonet-bounces@lists.utsouthwestern.edu] On =Behalf Of Amy Farnan [[8]Farnana@nehea=th.com] Sent: Friday, October 08, 2010 7:14 AM To: [9]histonet@lists.uts=uthwestern.edu Subject: [Histonet] mislabeling of slides and blocks Hello everyone, I was wondering if you could share your policy for employee infractions of =islabeling blocks and slides? I would like to get an idea of what the stan=dard is out there. Any help would be appreciated. Thank you Disclaimer: The information in this message is confidential. If you are no= the intended recipient, do not disclose, copy, or distribute this message= and please immediately contact the sender. _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouth=estern.edu [11]http:=/lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is fa=thful to the healing ministry of Jesus Christ, providing high quality care=or the body, mind and spirit of every person. The information contained in this communication, including attachments, is =onfidential and private and intended only for the use of the addressees. =nauthorized use, disclosure, distribution or copying is strictly prohibite= and may be unlawful. If you received this communication in error, please=inform us of the erroneous delivery by return e-mail message from your com=uter. Additionally, although all attachments have been scanned at the sou=ce for viruses, the recipient should check any attachments for the presenc= of viruses before opening. Alegent Health accepts no liability for any d=mage caused by any virus transmitted by this e-mail. Thank you for your c=operation. _______________________________________________ Histonet mailing list [12]Histonet@lists.utsouth=estern.edu [13]http:=/lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:Janice.Mahoney@alegent.org" 2. 3D"mailto:Farnana@nehealth.com" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet@lists.utsouthwestern.edu" 5. 3D"mailto:sherry.dean@ventana.roche.com" 6. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 7. 3D"mailto:histonet-bounces@l 8. 3D"mailto:Farnana@nehealth.com" 9. 3D"mailto:histonet@lists.utsouthwestern.edu" 10. 3D"mailto:Histonet@lists.utsouthwestern.edu" 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 12. 3D"mailto:Histonet@lists.utsouthwestern.edu" 13. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Fri Oct 8 13:39:28 2010 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Oct 8 13:40:05 2010 Subject: [Histonet] cassette labelers In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1394CA2C3C@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1394CA2C3C@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <00e701cb6718$2a89efb0$7f9dcf10$@com> In addition to Leica, TBS, and Thermo, there is also General Data and Brady. They each have their pro's and con's. If you'd like to hear more, please respond to me 'off line'. I would be shocked if any of these labelers cannot print 2 identifiers. The question really is how does this data get to the labeler? Does someone manually type it in or does it come from your LIS or tracking software? In the latter case, you need to ask your software vendor about their capabilities. P.S. I rarely see a name on a cassette. It's desired, but it just takes up too much space once you have a barcode and a case number on the cassette Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Friday, October 08, 2010 9:05 AM To: rsrichmond@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cassette labelers We have a new Leica/Surgipath cassette labeler after using the old Surgipath cassette labeler for approximately 10 years with no problems. Our Biomed would change the print head ever year or two because the pins would wear our. The new Leica/Surgipath labeler prints accession number and patient's name. You print cassettes one at a time and you can set the labeler to automatically advance the case number or block number. It's fast and easy! We love it!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, October 07, 2010 10:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labelers I'm advising a locum tenens client of mine about acquiring a cassette labeler. The only one I'm familiar with is Thermo Scientific's Cassette MicroWriter. Some questions for HistoNet: 1) A JCAHO inspector informed them that a cassette labeler will soon be required. Does anyone know if this is in fact the case? 2) Will the labeler print patients' names, or other second identifiers such as JCAHO now requires? 3) Are these labelers available in the used instrument market? 4) Is there any competition, or is the Thermo product all there is? 5) How well do these labelers withstand use? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thomas.crowell <@t> novartis.com Fri Oct 8 14:25:42 2010 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Fri Oct 8 14:25:48 2010 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 10/08/2010 and will not return until 10/12/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From sgoebel <@t> xbiotech.com Fri Oct 8 14:30:42 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 8 14:30:47 2010 Subject: [Histonet] mislabeling of slides and blocks Message-ID: <20101008123042.9e2d9aa830e8449a2412eb1e4f2f067e.5e689e78b2.wbe@email04.secureserver.net> Currrently I am working in a lab where I am the only tech., but in previous labs when mislabeling happened, the tech would be written up for it after one formal verbal warning. If they were written up 3 times in 6 months, then a suspension was possible (although never happens), then if the problem continued could lead to being fired. So, basically you would have to mislabel something or another 6 times in one year to be fired. Again...just my opinion... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] mislabeling of slides and blocks From: "Amy Farnan" Date: Fri, October 08, 2010 10:52 am To: "Janice A Mahoney" , Cc: "histonet@lists.utsouthwestern.edu" , "Sherry Dean" Sarah, What is your policy? From sfeher <@t> CMC-NH.ORG Fri Oct 8 16:07:28 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Oct 8 16:07:34 2010 Subject: [Histonet] cassette labelers In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1394CA2C3C@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1394CA2C3C@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F705@exchange.cmc-nh.org> We are using Leica's IPC and IPS slide labelers. We do not hand write labels but use bar code scanners to implement LEAN protocols and to add to our patient safety efforts. These units are set up as one would configure any other printer on a network. They can also be set up to operate on an individual PC. We set ours up with it's own CPU so it is recognized as a network printer by our LIS and can be accessed by anyone in the lab. The slide labeler has been configured to print the correct ThinPrep Imager required codes as well. A bar code and an accession number equate to two unique patient identifiers which complies with CAP and Joint Commission. Thermo has and Bradley have smaller units for low use areas. Leica's have a larger footprint but are primarily for high use labs. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Friday, October 08, 2010 10:05 AM To: rsrichmond@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cassette labelers We have a new Leica/Surgipath cassette labeler after using the old Surgipath cassette labeler for approximately 10 years with no problems. Our Biomed would change the print head ever year or two because the pins would wear our. The new Leica/Surgipath labeler prints accession number and patient's name. You print cassettes one at a time and you can set the labeler to automatically advance the case number or block number. It's fast and easy! We love it!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, October 07, 2010 10:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette labelers I'm advising a locum tenens client of mine about acquiring a cassette labeler. The only one I'm familiar with is Thermo Scientific's Cassette MicroWriter. Some questions for HistoNet: 1) A JCAHO inspector informed them that a cassette labeler will soon be required. Does anyone know if this is in fact the case? 2) Will the labeler print patients' names, or other second identifiers such as JCAHO now requires? 3) Are these labelers available in the used instrument market? 4) Is there any competition, or is the Thermo product all there is? 5) How well do these labelers withstand use? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Fri Oct 8 16:08:25 2010 From: kdboydhisto <@t> yahoo.com (kdboydhisto@yahoo.com) Date: Fri Oct 8 16:08:29 2010 Subject: [Histonet] To histonet!, very important information!! Message-ID: <344145.57840.qm@web58606.mail.re3.yahoo.com> hi! histonet! http://patinoires-synthetique.com/to.php Best regards, From Marilyn.A.Weiss <@t> kp.org Fri Oct 8 18:02:48 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Oct 8 18:03:28 2010 Subject: [Histonet] I will be out of office beginning the afternoon of 8/23 and returning 8/31 4 2010 and returning 8/13/2010 Message-ID: I will be out of the office starting 10/07/2010 and will not return until 10/11/2010. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. From cfitz <@t> 007group.com Fri Oct 8 21:22:06 2010 From: cfitz <@t> 007group.com (Cathy) Date: Fri Oct 8 21:22:13 2010 Subject: [Histonet] problems with staining IHC In-Reply-To: References: Message-ID: <505F7060B8054B1CA779A1CA1AD234F8@your8ba846406f> We and several other sites (in British Columbia and Alberta) that I have contacted have been experiencing IHC staining problems. Ours started in August while others have been seeing this since May. We have had conference calls with Ventana about this and they have said that it is a problem with the SuperFrost Plus slides; the slides seem to be hydrophobic. The staining that we are seeing is negative controls/positive patient, positive control/negative patient as well as variable counterstain. We have control tissue on every slide. From what we have learned from Fisher and Ventana they are working together with Erie Scientific (manufacturer of the Plus slides) to find out what is causing the problem. The slides appear to be vary from box to box and within each box. Some lot numbers are worse than others; our most recent lot number was 062510-9. I would suggest that you contact your sale person as well as the platform vendor (if your staining is automated) so they are aware of how large the problem may be. We have not found another slide that is any better than the Plus. Ventana is working on an official communication for their clients. We are very frustrated with the lack of progress in solving the problem. As anyone noticed that the Plus slides don't seem to have the same adhesive properties that they used to? Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital Kelowna, B.C. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Thursday, October 07, 2010 7:32 AM To: histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Sat Oct 9 12:07:37 2010 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Sat Oct 9 12:07:45 2010 Subject: [Histonet] mislabeling of slides and blocks In-Reply-To: <20101008123042.9e2d9aa830e8449a2412eb1e4f2f067e.5e689e78b2.wbe@email04.secureserver.net> References: <20101008123042.9e2d9aa830e8449a2412eb1e4f2f067e.5e689e78b2.wbe@email04.secureserver.net> Message-ID: If mistakes occur, the lab protocols must be altered to prevent the mistakes. If personnel can not or will not follow protocol to eliminate mistakes, then employment must be terminated. This is not being "picky" or unreasonable - ask any potential cancer patient relying on an accurate diagnosis. Tresa Goins Bozeman, Montana ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of sgoebel@xbiotech.com [sgoebel@xbiotech.com] Sent: Friday, October 08, 2010 1:30 PM To: Amy Farnan Cc: histonet@lists.utsouthwestern.edu; Sherry Dean Subject: RE: [Histonet] mislabeling of slides and blocks Currrently I am working in a lab where I am the only tech., but in previous labs when mislabeling happened, the tech would be written up for it after one formal verbal warning. If they were written up 3 times in 6 months, then a suspension was possible (although never happens), then if the problem continued could lead to being fired. So, basically you would have to mislabel something or another 6 times in one year to be fired. Again...just my opinion... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] mislabeling of slides and blocks From: "Amy Farnan" Date: Fri, October 08, 2010 10:52 am To: "Janice A Mahoney" , Cc: "histonet@lists.utsouthwestern.edu" , "Sherry Dean" Sarah, What is your policy? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plabruyere632 <@t> aol.com Sat Oct 9 20:04:20 2010 From: plabruyere632 <@t> aol.com (plabruyere632@aol.com) Date: Sat Oct 9 20:04:49 2010 Subject: [Histonet] Searching For Labs In Central Iowa Message-ID: <8CD362E3491ECC8-1DA0-199EE@webmail-d063.sysops.aol.com> I am looking to get back into the lab and dust off my skills. Was wondering if anyone had any type of information on labs that could use even volunteer type work in Central Iowa. Thanks, Paddie E. LaBruyere' From k84as <@t> yahoo.com Sun Oct 10 15:59:26 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sun Oct 10 16:06:12 2010 Subject: [Histonet] red and white muscle Message-ID: <837287.65097.qm@web112613.mail.gq1.yahoo.com> Dear all how can i differentiate between Red Muscle and white one (formalin fixed)? would you mind to share your protocol ? thanx From k84as <@t> yahoo.com Sun Oct 10 15:59:26 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sun Oct 10 16:26:12 2010 Subject: [Histonet] red and white muscle Message-ID: <837287.65097.qm@web112613.mail.gq1.yahoo.com> Dear all how can i differentiate between Red Muscle and white one (formalin fixed)? would you mind to share your protocol ? thanx From FARNANA <@t> nehealth.com Sun Oct 10 18:58:00 2010 From: FARNANA <@t> nehealth.com (Amy Farnan) Date: Sun Oct 10 18:58:07 2010 Subject: [Histonet] mislabeling of slides and blocks Message-ID: <4CB21AC8020000D9000405FD@neh_domain_app_srv.nehealth.com> Well, thank you to those that shared your policies. To those that just shared criticizism I wish you had shared your policies as well. It would have been more helpful and maybe others would have benefited as well. Remember folks the histonet if for sharing information not negativity. Again, thank you to those that shared or emailed me offline. >>> "Goins, Tresa" 10/09/10 1:08 PM >>> If mistakes occur, the lab protocols must be altered to prevent the mistakes. If personnel can not or will not follow protocol to eliminate mistakes, then employment must be terminated. This is not being "picky" or unreasonable - ask any potential cancer patient relying on an accurate diagnosis. Tresa Goins Bozeman, Montana ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of sgoebel@xbiotech.com [sgoebel@xbiotech.com] Sent: Friday, October 08, 2010 1:30 PM To: Amy Farnan Cc: histonet@lists.utsouthwestern.edu; Sherry Dean Subject: RE: [Histonet] mislabeling of slides and blocks Currrently I am working in a lab where I am the only tech., but in previous labs when mislabeling happened, the tech would be written up for it after one formal verbal warning. If they were written up 3 times in 6 months, then a suspension was possible (although never happens), then if the problem continued could lead to being fired. So, basically you would have to mislabel something or another 6 times in one year to be fired. Again...just my opinion... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] mislabeling of slides and blocks From: "Amy Farnan" Date: Fri, October 08, 2010 10:52 am To: "Janice A Mahoney" , Cc: "histonet@lists.utsouthwestern.edu" , "Sherry Dean" Sarah, What is your policy? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From cdisbrow <@t> msn.com Sun Oct 10 23:46:52 2010 From: cdisbrow <@t> msn.com (Carrie Disbrow) Date: Sun Oct 10 23:46:56 2010 Subject: [Histonet] Labeling issues In-Reply-To: References: Message-ID: Hi All, We are writing more numbers as the year goes..... S10-26,262 A1 - L1, has a combination of 12 numbers and letters with a hyphen and comma. Only three more numbers compared to the beginning of the year with S10-126 A1 - L1 but the potential for more mistakes. And what about the ergonomic issues of hand writing hundreds of slides per shift? And what about the stress of mislabeling that puts on the tech? Anyone ever done any serious studies in their labs to see when errors occur? How was the need for bar coding presented to your institutions to convince the department of the need for the equipment? Carrie From meljdelbarrio <@t> yahoo.com Mon Oct 11 01:42:26 2010 From: meljdelbarrio <@t> yahoo.com (Mel John del Barrio) Date: Mon Oct 11 01:42:32 2010 Subject: [Histonet] Xylol, Alcohol, Formalin Recycling Message-ID: <310131.95554.qm@web114508.mail.gq1.yahoo.com> Hi, Is there any sales reps/laboratory sup's here that can give me some info about recyling solvents and formalin? Thanks MJ Image by FlamingText.com From Melissa.Kuhnla <@t> chsli.org Mon Oct 11 05:26:28 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Mon Oct 11 05:26:35 2010 Subject: [Histonet] Labeling issues In-Reply-To: References: Message-ID: As a patient safety issue. We have had local hospitals end up in the new because of specimen mislabels. Very dangerous stuff. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Monday, October 11, 2010 12:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Labeling issues Hi All, We are writing more numbers as the year goes..... S10-26,262 A1 - L1, has a combination of 12 numbers and letters with a hyphen and comma. Only three more numbers compared to the beginning of the year with S10-126 A1 - L1 but the potential for more mistakes. And what about the ergonomic issues of hand writing hundreds of slides per shift? And what about the stress of mislabeling that puts on the tech? Anyone ever done any serious studies in their labs to see when errors occur? How was the need for bar coding presented to your institutions to convince the department of the need for the equipment? Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From CThornton <@t> dahlchase.com Mon Oct 11 07:11:41 2010 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon Oct 11 07:11:55 2010 Subject: [Histonet] problems with staining IHC In-Reply-To: <505F7060B8054B1CA779A1CA1AD234F8@your8ba846406f> References: <505F7060B8054B1CA779A1CA1AD234F8@your8ba846406f> Message-ID: Yes, we've been having the same problems with Erie slides. We use Ventana for IHC staining as well. We've actually met with Erie representatives and they took some of our "bad" lots back with them to test. They do admit there is a problem, I'm just not sure exactly what that problem is. We had issues in July/August, then September got much better, but last week we did over 30 repeats due to badly stained slides. It's frustrating, I know! We tried some other slides but they seemed to stain worse than the Erie. We're going to stick with Erie for now and just try to ride out the problems. At least now, we're very aware and extremely cautious, if something just doesn't look right we repeat it. Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Sent: Friday, October 08, 2010 10:22 PM To: srishan@mail.holyname.org; histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] problems with staining IHC We and several other sites (in British Columbia and Alberta) that I have contacted have been experiencing IHC staining problems. Ours started in August while others have been seeing this since May. We have had conference calls with Ventana about this and they have said that it is a problem with the SuperFrost Plus slides; the slides seem to be hydrophobic. The staining that we are seeing is negative controls/positive patient, positive control/negative patient as well as variable counterstain. We have control tissue on every slide. From what we have learned from Fisher and Ventana they are working together with Erie Scientific (manufacturer of the Plus slides) to find out what is causing the problem. The slides appear to be vary from box to box and within each box. Some lot numbers are worse than others; our most recent lot number was 062510-9. I would suggest that you contact your sale person as well as the platform vendor (if your staining is automated) so they are aware of how large the problem may be. We have not found another slide that is any better than the Plus. Ventana is working on an official communication for their clients. We are very frustrated with the lack of progress in solving the problem. As anyone noticed that the Plus slides don't seem to have the same adhesive properties that they used to? Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital Kelowna, B.C. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Thursday, October 07, 2010 7:32 AM To: histonet-bounces@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bridget.maryott <@t> ventana.roche.com Mon Oct 11 08:11:24 2010 From: bridget.maryott <@t> ventana.roche.com (Maryott, Bridget) Date: Mon Oct 11 08:11:36 2010 Subject: [Histonet] Leica EG1150 Tissue Embedding Center Message-ID: Is anyone using the new Leica EG1150 tissue embedding center? Any pros/cons? Is the cold plate area sufficient? Is it that much better than the EG1160? Thank you kindly for your time, Bridget Maryott, HT (ASCP) Advanced Staining Ventana Medical Systems, Inc. a member of the Roche Group 1910 E. Innovation Park Drive Tucson, Arizona 85755 Tel: +1 520 229 4022 mailto: bridget.maryott@ventana.roche.com Confidentiality Note: This message is intended only for the use of the named recipient(s) and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. From rmweber113 <@t> comcast.net Mon Oct 11 10:04:18 2010 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Mon Oct 11 10:04:21 2010 Subject: [Histonet] 2 DERM PATHOLOGIST NEEDED In-Reply-To: <873967628.72722.1286809264772.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: <1195172571.72975.1286809458010.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> I have 2 derm practices looking for a derm pathologist in Cumberland County NJ.? This will be a part time position for each.? One practice is at 3000 bx/yr and the other is 3500 bx/yr.? If you know of anyone interested please have them call at the number below. Thanks, Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 From laurie.colbert <@t> huntingtonhospital.com Mon Oct 11 10:08:24 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Oct 11 10:08:29 2010 Subject: [Histonet] Quality Assurance for Histology Message-ID: <57BE698966D5C54EAE8612E8941D768309B95CB3@EXCHANGE3.huntingtonhospital.com> I am revising our daily QA sheet that we hand out to the pathologists with the H&E's in the morning. I would like to gather some ideas from other sites. Does anyone have a form/chart that they would be willing to share with me? Laurie Colbert Huntington Hospital Pasadena CA From tpodawiltz <@t> lrgh.org Mon Oct 11 10:15:50 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Oct 11 10:15:56 2010 Subject: [Histonet] mislabeling of slides and blocks In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CD99928@EXCHMBC2.ad.ah.local> References: <4CAED2F0.26ED.00D9.1@nehealth.com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CD99928@EXCHMBC2.ad.ah.local> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5DF8392@LRGHEXVS1.practice.lrgh.org> So who gets fired the person that made the error or the person who let the slide out of the lab after verifying that the block matched the number and tissue on the slide? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Friday, October 08, 2010 12:41 PM To: Amy Farnan; histonet@lists.utsouthwestern.edu Cc: Dean, Sherry Subject: RE: [Histonet] mislabeling of slides and blocks I'd be happy to share mine but it is very strict. People are given a final written warning for the first mislabel not caught in the department and can be fired for the second within a year. I highly suggest the Ventana Vantage (there are others but in my opinion Vantage is the best) bar code system for patient safety. It also relieves the stress on your Histo techs because the bar coding helps them assure they have the correct slide, block, etc. Techs are hard to find and if we start firing for inevitable human error we will cause even more tech shortages. When you look at all the costs that a mislabel can incur, (law suits, replacing personnel, rework, etc.) the cost of the software is not too bad. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Farnan [Farnana@nehealth.com] Sent: Friday, October 08, 2010 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mislabeling of slides and blocks Hello everyone, I was wondering if you could share your policy for employee infractions of mislabeling blocks and slides? I would like to get an idea of what the standard is out there. Any help would be appreciated. Thank you Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From rjbuesa <@t> yahoo.com Mon Oct 11 10:21:09 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 11 10:21:15 2010 Subject: [Histonet] Quality Assurance for Histology In-Reply-To: <57BE698966D5C54EAE8612E8941D768309B95CB3@EXCHANGE3.huntingtonhospital.com> Message-ID: <403454.19447.qm@web65705.mail.ac4.yahoo.com> After many different forms and many efforts to make the pathologists to provide feed back about the quality of the sections and procedures, this is what I finished doing: to ask the pathologists to simply separate the slides they considered of poor quality and those unacceptable for diagnoses. It was then my job to define the problem and to addressed it with the histotech who made the slide, to determine the re-training or any other administrative action deemed necessary. After I did that I started to receive slides?while before seldom any pathologist was willing to use any time to evaluate the slides. In all reality they are quite busy to take time to fill forms that, in any event, I also had to review, re-evaluate and discuss with the histotech. This procedure worked very well for me and the quality of the work was improved considerably, as well as the rejections diminished. Try this approach. Ren? J. --- On Mon, 10/11/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Quality Assurance for Histology To: histonet@lists.utsouthwestern.edu Date: Monday, October 11, 2010, 11:08 AM I am revising our daily QA sheet that we hand out to the pathologists with the H&E's in the morning. I would like to gather some ideas from other sites.? Does anyone have a form/chart that they would be willing to share with me? Laurie Colbert Huntington Hospital Pasadena CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Mon Oct 11 10:25:46 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Oct 11 10:27:15 2010 Subject: [Histonet] mislabeling of slides and blocks In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323D5DF8392@LRGHEXVS1.practice.lrgh.org> References: <4CAED2F0.26ED.00D9.1@nehealth.com> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CD99928@EXCHMBC2.ad.ah.local> <38667E7FB77ECD4E91BFAEB8D986386323D5DF8392@LRGHEXVS1.practice.lrgh.org> Message-ID: I would say the person who did the QC.... After all, isn't that their job? But then again, 2 mislabeled slides does not justify termination (well, depending on the frequency)... I guess I would be looking at performance improvement. Janice hit the nail on the head and is very right... bar-coding is the way to go! That was one of the big topics at this year's NSH. Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, October 11, 2010 8:16 AM To: 'Mahoney,Janice A'; Amy Farnan; histonet@lists.utsouthwestern.edu Cc: Dean, Sherry Subject: RE: [Histonet] mislabeling of slides and blocks So who gets fired the person that made the error or the person who let the slide out of the lab after verifying that the block matched the number and tissue on the slide? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Friday, October 08, 2010 12:41 PM To: Amy Farnan; histonet@lists.utsouthwestern.edu Cc: Dean, Sherry Subject: RE: [Histonet] mislabeling of slides and blocks I'd be happy to share mine but it is very strict. People are given a final written warning for the first mislabel not caught in the department and can be fired for the second within a year. I highly suggest the Ventana Vantage (there are others but in my opinion Vantage is the best) bar code system for patient safety. It also relieves the stress on your Histo techs because the bar coding helps them assure they have the correct slide, block, etc. Techs are hard to find and if we start firing for inevitable human error we will cause even more tech shortages. When you look at all the costs that a mislabel can incur, (law suits, replacing personnel, rework, etc.) the cost of the software is not too bad. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Farnan [Farnana@nehealth.com] Sent: Friday, October 08, 2010 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mislabeling of slides and blocks Hello everyone, I was wondering if you could share your policy for employee infractions of mislabeling blocks and slides? I would like to get an idea of what the standard is out there. Any help would be appreciated. Thank you Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From tpodawiltz <@t> lrgh.org Mon Oct 11 10:27:40 2010 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Oct 11 10:30:28 2010 Subject: [Histonet] Quality Assurance for Histology In-Reply-To: <403454.19447.qm@web65705.mail.ac4.yahoo.com> References: <57BE698966D5C54EAE8612E8941D768309B95CB3@EXCHANGE3.huntingtonhospital.com> <403454.19447.qm@web65705.mail.ac4.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323D5DF8394@LRGHEXVS1.practice.lrgh.org> I actually randomly review the slides before they are sent to the Pathologist any slide with incomplete sections, chatter or other major defects get re-cut at that time. Since doing this complaints from the Pathologist disappeared about the quality of the slides they were getting. They get the QA form with the last book of slides for the day. They fill it out then give it back to me. Works well for us. I do know this will not work for others, but it works for us. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, October 11, 2010 11:21 AM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Quality Assurance for Histology After many different forms and many efforts to make the pathologists to provide feed back about the quality of the sections and procedures, this is what I finished doing: to ask the pathologists to simply separate the slides they considered of poor quality and those unacceptable for diagnoses. It was then my job to define the problem and to addressed it with the histotech who made the slide, to determine the re-training or any other administrative action deemed necessary. After I did that I started to receive slides?while before seldom any pathologist was willing to use any time to evaluate the slides. In all reality they are quite busy to take time to fill forms that, in any event, I also had to review, re-evaluate and discuss with the histotech. This procedure worked very well for me and the quality of the work was improved considerably, as well as the rejections diminished. Try this approach. Ren? J. --- On Mon, 10/11/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Quality Assurance for Histology To: histonet@lists.utsouthwestern.edu Date: Monday, October 11, 2010, 11:08 AM I am revising our daily QA sheet that we hand out to the pathologists with the H&E's in the morning. I would like to gather some ideas from other sites.? Does anyone have a form/chart that they would be willing to share with me? Laurie Colbert Huntington Hospital Pasadena CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From patrick.lewis <@t> seattlechildrens.org Mon Oct 11 10:35:27 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Mon Oct 11 10:35:58 2010 Subject: [Histonet] Superforst plus slides issues Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C05E8508E@s107.childrens.sea.kids> Hi histonetters Has anyone got more info on the superfrost plus poor quality issue? Are there specific lot # that are going to be recalled? Is there a way to find out if any of our boxes of slides are likely to be bad/poor adherence? I just started doing some IHCs and I am crossing my fingers that my slides are ok. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Bill.Tench <@t> pph.org Mon Oct 11 11:02:01 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Mon Oct 11 11:02:08 2010 Subject: [Histonet] Quality Assurance for Histology In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323D5DF8394@LRGHEXVS1.practice.lrgh.org> References: <57BE698966D5C54EAE8612E8941D768309B95CB3@EXCHANGE3.huntingtonhospital.com><403454.19447.qm@web65705.mail.ac4.yahoo.com> <38667E7FB77ECD4E91BFAEB8D986386323D5DF8394@LRGHEXVS1.practice.lrgh.org> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56A1@MAIL1.pph.local> Why would you want to have the pathologists fill out a QA sheet for a function you have already performed (and should document). This would seem to be a meaningless exercise (ie, waste of time) for the pathologist. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, October 11, 2010 8:28 AM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: RE: [Histonet] Quality Assurance for Histology I actually randomly review the slides before they are sent to the Pathologist any slide with incomplete sections, chatter or other major defects get re-cut at that time. Since doing this complaints from the Pathologist disappeared about the quality of the slides they were getting. They get the QA form with the last book of slides for the day. They fill it out then give it back to me. Works well for us. I do know this will not work for others, but it works for us. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, October 11, 2010 11:21 AM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Quality Assurance for Histology After many different forms and many efforts to make the pathologists to provide feed back about the quality of the sections and procedures, this is what I finished doing: to ask the pathologists to simply separate the slides they considered of poor quality and those unacceptable for diagnoses. It was then my job to define the problem and to addressed it with the histotech who made the slide, to determine the re-training or any other administrative action deemed necessary. After I did that I started to receive slides?while before seldom any pathologist was willing to use any time to evaluate the slides. In all reality they are quite busy to take time to fill forms that, in any event, I also had to review, re-evaluate and discuss with the histotech. This procedure worked very well for me and the quality of the work was improved considerably, as well as the rejections diminished. Try this approach. Ren? J. --- On Mon, 10/11/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Quality Assurance for Histology To: histonet@lists.utsouthwestern.edu Date: Monday, October 11, 2010, 11:08 AM I am revising our daily QA sheet that we hand out to the pathologists with the H&E's in the morning. I would like to gather some ideas from other sites.? Does anyone have a form/chart that they would be willing to share with me? Laurie Colbert Huntington Hospital Pasadena CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From JEllin <@t> yumaregional.org Mon Oct 11 11:06:13 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Oct 11 11:05:19 2010 Subject: [Histonet] Quality Assurance for Histology In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A56A1@MAIL1.pph.local> References: <57BE698966D5C54EAE8612E8941D768309B95CB3@EXCHANGE3.huntingtonhospital.com><403454.19447.qm@web65705.mail.ac4.yahoo.com><38667E7FB77ECD4E91BFAEB8D986386323D5DF8394@LRGHEXVS1.practice.lrgh.org> <2820431BF953BB4DA3E9E1A5882265FD034A56A1@MAIL1.pph.local> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68BA@EXCHANGECLUSTER.yumaregional.local> We have a place in out LIS that the Pathologist can choose and document everything, then we run a report that tabulates and give us the values and solutions. One thing I am seeing here is that we also need to address the solution. A lot of people that I have inspected do great with marking the issues, but solutions and tracking they do not do well. Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Monday, October 11, 2010 9:02 AM To: Podawiltz, Thomas; Rene J Buesa; histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: RE: [Histonet] Quality Assurance for Histology Why would you want to have the pathologists fill out a QA sheet for a function you have already performed (and should document). This would seem to be a meaningless exercise (ie, waste of time) for the pathologist. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, October 11, 2010 8:28 AM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: RE: [Histonet] Quality Assurance for Histology I actually randomly review the slides before they are sent to the Pathologist any slide with incomplete sections, chatter or other major defects get re-cut at that time. Since doing this complaints from the Pathologist disappeared about the quality of the slides they were getting. They get the QA form with the last book of slides for the day. They fill it out then give it back to me. Works well for us. I do know this will not work for others, but it works for us. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, October 11, 2010 11:21 AM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Quality Assurance for Histology After many different forms and many efforts to make the pathologists to provide feed back about the quality of the sections and procedures, this is what I finished doing: to ask the pathologists to simply separate the slides they considered of poor quality and those unacceptable for diagnoses. It was then my job to define the problem and to addressed it with the histotech who made the slide, to determine the re-training or any other administrative action deemed necessary. After I did that I started to receive slides?while before seldom any pathologist was willing to use any time to evaluate the slides. In all reality they are quite busy to take time to fill forms that, in any event, I also had to review, re-evaluate and discuss with the histotech. This procedure worked very well for me and the quality of the work was improved considerably, as well as the rejections diminished. Try this approach. Ren? J. --- On Mon, 10/11/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Quality Assurance for Histology To: histonet@lists.utsouthwestern.edu Date: Monday, October 11, 2010, 11:08 AM I am revising our daily QA sheet that we hand out to the pathologists with the H&E's in the morning. I would like to gather some ideas from other sites.? Does anyone have a form/chart that they would be willing to share with me? Laurie Colbert Huntington Hospital Pasadena CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From laurie.colbert <@t> huntingtonhospital.com Mon Oct 11 11:06:51 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Oct 11 11:06:57 2010 Subject: [Histonet] Quality Assurance for Histology In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A56A1@MAIL1.pph.local> Message-ID: <57BE698966D5C54EAE8612E8941D768309B95CE6@EXCHANGE3.huntingtonhospital.com> Actually, it is a CAP requirement: ANP.11713 "There is documented evidence of daily review of the technical quality of histologic preparations by the pathologist." -----Original Message----- From: Tench, Bill [mailto:Bill.Tench@pph.org] Sent: Monday, October 11, 2010 9:02 AM To: Podawiltz, Thomas; Rene J Buesa; histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: RE: [Histonet] Quality Assurance for Histology Why would you want to have the pathologists fill out a QA sheet for a function you have already performed (and should document). This would seem to be a meaningless exercise (ie, waste of time) for the pathologist. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, October 11, 2010 8:28 AM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: RE: [Histonet] Quality Assurance for Histology I actually randomly review the slides before they are sent to the Pathologist any slide with incomplete sections, chatter or other major defects get re-cut at that time. Since doing this complaints from the Pathologist disappeared about the quality of the slides they were getting. They get the QA form with the last book of slides for the day. They fill it out then give it back to me. Works well for us. I do know this will not work for others, but it works for us. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, October 11, 2010 11:21 AM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Quality Assurance for Histology After many different forms and many efforts to make the pathologists to provide feed back about the quality of the sections and procedures, this is what I finished doing: to ask the pathologists to simply separate the slides they considered of poor quality and those unacceptable for diagnoses. It was then my job to define the problem and to addressed it with the histotech who made the slide, to determine the re-training or any other administrative action deemed necessary. After I did that I started to receive slides?while before seldom any pathologist was willing to use any time to evaluate the slides. In all reality they are quite busy to take time to fill forms that, in any event, I also had to review, re-evaluate and discuss with the histotech. This procedure worked very well for me and the quality of the work was improved considerably, as well as the rejections diminished. Try this approach. Ren? J. --- On Mon, 10/11/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Quality Assurance for Histology To: histonet@lists.utsouthwestern.edu Date: Monday, October 11, 2010, 11:08 AM I am revising our daily QA sheet that we hand out to the pathologists with the H&E's in the morning. I would like to gather some ideas from other sites.? Does anyone have a form/chart that they would be willing to share with me? Laurie Colbert Huntington Hospital Pasadena CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From Bill.Tench <@t> pph.org Mon Oct 11 11:25:14 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Mon Oct 11 11:25:19 2010 Subject: [Histonet] Quality Assurance for Histology In-Reply-To: <57BE698966D5C54EAE8612E8941D768309B95CE6@EXCHANGE3.huntingtonhospital.com> References: <2820431BF953BB4DA3E9E1A5882265FD034A56A1@MAIL1.pph.local> <57BE698966D5C54EAE8612E8941D768309B95CE6@EXCHANGE3.huntingtonhospital.com> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56A4@MAIL1.pph.local> Sorry, you are right about that. But, one wonders, if the problem has been solved before they get the slides, what's the usefulness of the activity (that would be a CAP debate) Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Monday, October 11, 2010 9:07 AM To: Tench, Bill; Podawiltz, Thomas; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Quality Assurance for Histology Actually, it is a CAP requirement: ANP.11713 "There is documented evidence of daily review of the technical quality of histologic preparations by the pathologist." -----Original Message----- From: Tench, Bill [mailto:Bill.Tench@pph.org] Sent: Monday, October 11, 2010 9:02 AM To: Podawiltz, Thomas; Rene J Buesa; histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: RE: [Histonet] Quality Assurance for Histology Why would you want to have the pathologists fill out a QA sheet for a function you have already performed (and should document). This would seem to be a meaningless exercise (ie, waste of time) for the pathologist. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, October 11, 2010 8:28 AM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: RE: [Histonet] Quality Assurance for Histology I actually randomly review the slides before they are sent to the Pathologist any slide with incomplete sections, chatter or other major defects get re-cut at that time. Since doing this complaints from the Pathologist disappeared about the quality of the slides they were getting. They get the QA form with the last book of slides for the day. They fill it out then give it back to me. Works well for us. I do know this will not work for others, but it works for us. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, October 11, 2010 11:21 AM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: Re: [Histonet] Quality Assurance for Histology After many different forms and many efforts to make the pathologists to provide feed back about the quality of the sections and procedures, this is what I finished doing: to ask the pathologists to simply separate the slides they considered of poor quality and those unacceptable for diagnoses. It was then my job to define the problem and to addressed it with the histotech who made the slide, to determine the re-training or any other administrative action deemed necessary. After I did that I started to receive slides?while before seldom any pathologist was willing to use any time to evaluate the slides. In all reality they are quite busy to take time to fill forms that, in any event, I also had to review, re-evaluate and discuss with the histotech. This procedure worked very well for me and the quality of the work was improved considerably, as well as the rejections diminished. Try this approach. Ren? J. --- On Mon, 10/11/10, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Quality Assurance for Histology To: histonet@lists.utsouthwestern.edu Date: Monday, October 11, 2010, 11:08 AM I am revising our daily QA sheet that we hand out to the pathologists with the H&E's in the morning. I would like to gather some ideas from other sites.? Does anyone have a form/chart that they would be willing to share with me? Laurie Colbert Huntington Hospital Pasadena CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From jclark <@t> pcnm.com Mon Oct 11 11:33:03 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon Oct 11 11:33:10 2010 Subject: [Histonet] RE: Histonet Digest, Vol 83, Issue 13 Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C0139A10B@mail.pcnm.com> We use the CBG recycling system and it does a great job. It will do alcohol, xylene and formalin; however, we do not bother with the formalin since to have to re-pH it after (too much of a hassle when formalin is so cheap to purchase). Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Message: 5 Date: Sun, 10 Oct 2010 23:42:26 -0700 (PDT) From: Mel John del Barrio Subject: [Histonet] Xylol, Alcohol, Formalin Recycling To: histonet@lists.utsouthwestern.edu Message-ID: <310131.95554.qm@web114508.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Hi, Is there any sales reps/laboratory sup's here that can give me some info about recyling solvents and formalin? Thanks MJ Image by FlamingText.com ------------------------------ From rsrichmond <@t> gmail.com Mon Oct 11 11:51:13 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Oct 11 11:51:20 2010 Subject: [Histonet] Re: Quality Assurance for Histology Message-ID: Quality Assurance for Histology: I've been a locum tenens pathologist for nearly 30 years, and have worked on more than 60 pathology services in my "career". I'll make some melancholy observations on this subject. The more "quality assurance" paperwork I have to do, the worse the quality of the slides. I always get a lot of angry response when I say this on Histonet, but I assure you that the great majority of small pathology service histotechs take great pride in not having a microscope and never looking at a slide. The most common problems I see are excessive variability of staining, and venetian-blind artifact in Gi biopsy sections. (I don't see problems with inadequate processing, because I don't overload cassettes and I insist on adequate time for fixation.) If I ran the zoo, I would want to review some selected slides with a senior histotech at the end of every day, using a double-headed microscope. Some written record would need to be generated, I imagine. I have NEVER seen this sort of review done. Pathologists bear the ultimate responsibility for solving these problems. Residency programs have been completely ineffective in training pathologists how to do this. I wish I knew more about how radiology services do quality assurance. I notice that I don't see out-of-focus X-ray films. The MBA's drove Edwards Deming to Japan more than half a century ago rather than adopt it, but effective feedback is necessary in any manufacturing process. Bob Richmond Samurai Pathologist Knoxville TN From JWeems <@t> sjha.org Mon Oct 11 12:02:52 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Oct 11 12:02:58 2010 Subject: [Histonet] Re: Quality Assurance for Histology In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640398778058@CHEXCMS10.one.ads.che.org> I wish more pathologists had your mindset... Sigh... J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, October 11, 2010 12:51 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Quality Assurance for Histology Quality Assurance for Histology: I've been a locum tenens pathologist for nearly 30 years, and have worked on more than 60 pathology services in my "career". I'll make some melancholy observations on this subject. The more "quality assurance" paperwork I have to do, the worse the quality of the slides. I always get a lot of angry response when I say this on Histonet, but I assure you that the great majority of small pathology service histotechs take great pride in not having a microscope and never looking at a slide. The most common problems I see are excessive variability of staining, and venetian-blind artifact in Gi biopsy sections. (I don't see problems with inadequate processing, because I don't overload cassettes and I insist on adequate time for fixation.) If I ran the zoo, I would want to review some selected slides with a senior histotech at the end of every day, using a double-headed microscope. Some written record would need to be generated, I imagine. I have NEVER seen this sort of review done. Pathologists bear the ultimate responsibility for solving these problems. Residency programs have been completely ineffective in training pathologists how to do this. I wish I knew more about how radiology services do quality assurance. I notice that I don't see out-of-focus X-ray films. The MBA's drove Edwards Deming to Japan more than half a century ago rather than adopt it, but effective feedback is necessary in any manufacturing process. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rsrichmond <@t> gmail.com Mon Oct 11 12:14:40 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Oct 11 12:14:43 2010 Subject: [Histonet] Re: Quality Assurance for Histology In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640398778058@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640398778058@CHEXCMS10.one.ads.che.org> Message-ID: Quality Assurance for Histology: I meant to add: one way pathologists are part of the problem when they think they are part of the solution is - and I am very frequently guilty of this - is thinking "This is a totally crappy slide, but it makes the diagnosis, so what the hey, let's push it on out the door." When I do this, Edwards Deming worked in vain. Bob Richmond Samurai Pathologist Knoxville TN From sgoebel <@t> xbiotech.com Mon Oct 11 12:15:46 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Oct 11 12:15:51 2010 Subject: [Histonet] Pathologists vs. Surgeons Message-ID: <20101011101546.9e2d9aa830e8449a2412eb1e4f2f067e.02888e5b02.wbe@email04.secureserver.net> Hey Ya'll, A friend of mine forwarded me this. We both are HTs and have had to deal with frozens a lot on a daily basis. Thought everyone might need a good chuckle on a Monday!! Cheers!! http://www.xtranormal.com/watch/6847761/ Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 From Beth.Fye <@t> HCAhealthcare.com Mon Oct 11 12:36:02 2010 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Mon Oct 11 12:36:08 2010 Subject: [Histonet] Quality Assurance for Histology Message-ID: <938F8EC5A524D34EB5796E23E52781D34E9E344C6F@NADCWPMSGCMS05.hca.corpad.net> This is the form that we use. We have a separate log sheet for H&E control slides done each morning. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From Beth.Fye <@t> HCAhealthcare.com Mon Oct 11 12:49:29 2010 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Mon Oct 11 12:49:39 2010 Subject: [Histonet] FW: Quality Assurance for Histology Message-ID: <938F8EC5A524D34EB5796E23E52781D34E9E344CB2@NADCWPMSGCMS05.hca.corpad.net> I guess the attachment didn't work. So I will copy and paste. I hope the formatting does not get misaligned. QUALITY ASSURANCE HISTOLOGY SLIDES DATE: STAINING: POOR GOOD EXCELLENT COMMENTS: ARTIFACTS: TISSUE TEARS: VENETIAN BLIND EFFECT: NONE FEW MANY OTHERS: LABELING ERRORS: PHYSICIAN'S COMMENTS: CORRECTIVE ACTION: PHYSICIAN SIGNATURE: H&E CONTROL SLIDE: DIFF QUIK CONTROL SLIDE: CASE RANGE: Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _____________________________________________ From: Fye Beth Sent: Monday, October 11, 2010 1:36 PM To: 'Laurie Colbert' Cc: 'histonet@lists.utsouthwestern.edu' Subject: Quality Assurance for Histology This is the form that we use. We have a separate log sheet for H&E control slides done each morning. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From Beth.Fye <@t> HCAhealthcare.com Mon Oct 11 13:43:12 2010 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Mon Oct 11 13:43:18 2010 Subject: [Histonet] RE: Quality Assurance for Histology In-Reply-To: <57BE698966D5C54EAE8612E8941D768309B95D9F@EXCHANGE3.huntingtonhospital.com> References: <938F8EC5A524D34EB5796E23E52781D34E9E344CB2@NADCWPMSGCMS05.hca.corpad.net> <57BE698966D5C54EAE8612E8941D768309B95D9F@EXCHANGE3.huntingtonhospital.com> Message-ID: <938F8EC5A524D34EB5796E23E52781D34E9E344DE7@NADCWPMSGCMS05.hca.corpad.net> I forgot to mention that we do Histology for 3 different sites. This form is sent to all 3 sites. It is interesting to see the difference in the grading of the stain between pathologists on the same day from the same stainer. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Monday, October 11, 2010 2:40 PM To: Fye Beth Subject: RE: Quality Assurance for Histology Thanks! ________________________________ From: Beth.Fye@HCAhealthcare.com [mailto:Beth.Fye@HCAhealthcare.com] Sent: Monday, October 11, 2010 10:49 AM To: histonet@lists.utsouthwestern.edu; Laurie Colbert Subject: FW: Quality Assurance for Histology I guess the attachment didn't work. So I will copy and paste. I hope the formatting does not get misaligned. QUALITY ASSURANCE HISTOLOGY SLIDES DATE: STAINING: POOR GOOD EXCELLENT COMMENTS: ARTIFACTS: TISSUE TEARS: VENETIAN BLIND EFFECT: NONE FEW MANY OTHERS: LABELING ERRORS: PHYSICIAN'S COMMENTS: CORRECTIVE ACTION: PHYSICIAN SIGNATURE: H&E CONTROL SLIDE: DIFF QUIK CONTROL SLIDE: CASE RANGE: Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _____________________________________________ From: Fye Beth Sent: Monday, October 11, 2010 1:36 PM To: 'Laurie Colbert' Cc: 'histonet@lists.utsouthwestern.edu' Subject: Quality Assurance for Histology This is the form that we use. We have a separate log sheet for H&E control slides done each morning. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From foreightl <@t> gmail.com Mon Oct 11 16:42:14 2010 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Mon Oct 11 16:42:20 2010 Subject: [Histonet] Molecular & FISH technologist needed in Seattle, WA Message-ID: *Molecular & FISH technologist* * * *SUMMARY* Under the supervision of the Histology Supervisor the Molecular & FISH Technologist performs the tests and procedures assigned to the Molecular Pathology Department. The position is responsible for specimen accessioning, preservation, processing, testing, reagent preparation, quality assurance, equipment maintenance, operation, reading of slides, initial report preparation and communication with clients and pathologists and all other duties assigned by the Supervisor. * * *ESSENTIAL FUNCTIONS* ? Performs all routine and complex special molecular and FISH procedures with the understanding of molecular diseases, cellular and tissue structures, techniques, principles, theory and instrumentation. ? With general direction from the Medical Director and the Molecular consultant is responsible for set up and validation of new FISH procedures. ? Assures specimen integrity, labeling, and preservation requirements are met and gathers necessary clinical information as needed. ? Fully responsible for operation, troubleshooting and maintenance of the molecular and FISH related equipment in the department. Completes all instrument function verification, maintenance, and documents according to procedure in department. Ensure that equipment defects and malfunctions are reported and repaired. ? Demonstrates general knowledge of pathological and physiological conditions that affect test results and tissue staining. ? Uses and documents controls, proficiency test samples and maintains quality assurance records appropriately. Monitors quality control results and takes immediate and proper action when controls are unacceptable. Follow defined procedures with only supervisor or pathologist approved modifications or deviations. ? Recognizes and troubleshoots routine and complex problems and assist other department staff and lab assistants with technical problems. ? Responsible for the proper handling and disposal of all biohazardous materials and chemically hazardous materials including the neutralization or recycling of chemicals before disposal or reuse. Always maintains a safe work environment and attends all safety training classes and conforms to all company safety guidelines and requirements. ? Takes on additional responsibility for training Laboratory personnel including Laboratory Assistants, NRT?s and other Histotechs in molecular and FISH procedures. ? Will be responsible for writing/updating Operating Procedures. ? Assists supervisory staff in monitoring workflow and insuring that work priorities are met for the department. ? Participates in special projects or other duties as assigned by supervisor staff or company. ? Always maintains a pleasant, courteous attitude when answering the telephone. ? Participates in weekend and holiday schedules as staffing requirements dictate. Remains flexible and works a share of overtime or different shifts if necessary during staffing shortages or emergencies. ? Participates in continuing education classes and courses. Strongly encouraged to keep updated on recent advances in the field of FISH and to take at least 10 credit hours of continuing education a year. ? Conforms to established and new procedures and policies instituted by the company. * * *EDUCATION and/or EXPERIENCE* ? MT (ASCP) or equivalent, Canadian or Foreign registered with college degree required. ? 3-5 years experience in molecular techniques and FISH method set up, validation and screening/reading of FISH slides required. ? Experience using automated imaging and FISH analysis software required. Please send Resume or CV to: careers@cellnetix.com Thanks, -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From k84as <@t> yahoo.com Mon Oct 11 17:31:12 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Mon Oct 11 17:31:47 2010 Subject: [Histonet] red and white muscle? Message-ID: <403630.74784.qm@web112601.mail.gq1.yahoo.com> Dear all how can i differentiate between Red Muscle and white one (formalin fixed)? would you mind to share your protocol ? thanx From Beth.Fye <@t> HCAhealthcare.com Tue Oct 12 09:01:23 2010 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Tue Oct 12 09:01:31 2010 Subject: [Histonet] Defrosting Cryostat Message-ID: <938F8EC5A524D34EB5796E23E52781D34E9E345412@NADCWPMSGCMS05.hca.corpad.net> How are institutions handling the requirement for defrosting the cryostat during disinfection? Do most sites have a spare cryostat to use when one is defrosting or do you pay staff to come in on the weekends to do this? I know this requirement has been out for over a year and hoping for some good feedback now. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From trathborne <@t> somerset-healthcare.com Tue Oct 12 09:06:43 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Oct 12 09:06:50 2010 Subject: [Histonet] Defrosting Cryostat In-Reply-To: <938F8EC5A524D34EB5796E23E52781D34E9E345412@NADCWPMSGCMS05.hca.corpad.net> Message-ID: We have a second cryostat. This is also used during our scheduled pm's each year when a contracted company comes in for this purpose. Since we have frozens most days, we really can't be without a cryostat. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Tuesday, October 12, 2010 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Defrosting Cryostat How are institutions handling the requirement for defrosting the cryostat during disinfection? Do most sites have a spare cryostat to use when one is defrosting or do you pay staff to come in on the weekends to do this? I know this requirement has been out for over a year and hoping for some good feedback now. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From barrickstacey <@t> yahoo.com Tue Oct 12 09:20:07 2010 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Tue Oct 12 09:20:19 2010 Subject: [Histonet] SDS in ICC protocols? Message-ID: <867288.4551.qm@web54304.mail.re2.yahoo.com> Hello, Has anyone ever used SDS along with trition in ICC protocols? We see very different results when we use Triton alone without SDS. Thanks!! From talulahgosh <@t> gmail.com Tue Oct 12 09:31:20 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Oct 12 09:31:26 2010 Subject: [Histonet] Defrosting Cryostat In-Reply-To: <938F8EC5A524D34EB5796E23E52781D34E9E345412@NADCWPMSGCMS05.hca.corpad.net> References: <938F8EC5A524D34EB5796E23E52781D34E9E345412@NADCWPMSGCMS05.hca.corpad.net> Message-ID: Our cryostat is a CM3050--we let it defrost overnight with the glass door open and it's fine the next morning. alternatively, you could defrost it over a weekend. i don't know how long disinfecting takes, though, so this may not be feasible for you. also, it only takes a few hours more to get back down to the working temperature, so it's ready to use the next day, just later, at say noon or so. Emily -- Correction: This blog post originally stated that one in three black men who have sex with me is HIV positive. In fact, the statistic applies to black men who have sex with men. http://www.tbd.com/blogs/amanda-hess/2010/10/hiv-positive-black-gay-men-to-get-the-bayard-rustin-project-a-district-campaign-against-aids-2873.html From sgoebel <@t> xbiotech.com Tue Oct 12 09:33:42 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Oct 12 09:33:47 2010 Subject: [Histonet] Defrosting Cryostat Message-ID: <20101012073342.9e2d9aa830e8449a2412eb1e4f2f067e.494f9c7598.wbe@email04.secureserver.net> With one cryostat it is a little bit difficult, but not impossibl Turn it off at the end of the day (after all the possible frozen s urgeries are done) and let it defrost overnight. Have someone come in or both minutes or s time then just co dealt with never come Good luck!! < Sarah Goeb Histotechnician XBiotech 8201 East Riverside Dr. Bldg 4 Suite 100< Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] Defrosting Cryostat From: <[1]Beth.Fye@HCAhealthc Date: Tue, October 12, 2010 7:01 am To: <[2]histonet@lists How are institutions handling the requirement for defrosting the cryostat d use when one i weekends to do this? I year and hoping for some good Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 <[3]mailto:Beth.Fye@hcahealth _______________________________________________ Histonet mailing list [4]Histonet@lists.utsouth [5]http: References 1. 3D"mailto:Beth.Fye@HCAhealthcare.com" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Beth.Fye@hcahealthcare.com" 4. 3D"mailto:Histonet@lists.utsouthwestern.edu" 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From relia1 <@t> earthlink.net Tue Oct 12 09:47:24 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Oct 12 09:47:37 2010 Subject: [Histonet] RELIA Histology Careers Bulletin 10-12-10 Did you make it to the NSH in Seattle this year? Message-ID: Hello Histonetters!, How are you doing? Did you make it to the NSH in Seattle? If so how was it? What was your favorite thing about it? I haven?t been since the convention in 2005 in Ft. Lauderdale. I really enjoyed meeting people, learning new things and the party at the Hard Rock Casino. I am planning on attending the 2011 meeting. Will I see you there? While I have your attention ;0) here is a list of my current openings all of the positions are full time permanent positions. And my clients offer excellent compensation, benefits and relocation assistance. And they are ready to interview and hire right away! Here is a list of my current openings: HISTOLOGY SUPERVISORS/MANAGERS: Histology Lab Manager Central Valley CA Director of Quality Assurance ? Orange/Rockland County NY HISTOTECHNICIANS/HISTOTECHNOLOGISTS: TN ? Nashville Histotech day and night shifts avail. IN ? South of Chicago in Northern IN Histology Tech GA ? Southern Coastal area ? Histotech with IHC NY-Orange/Rockland County Brand New Lab NYS license/Elig day shift TX ? Dallas Night shift IHC exper req MA ? Boston Area several shifts available dermpath and general MA ? Cape Cod ASCP or elig. Awesome Hospital Lab. FL ? Orlando FL Technologist license required. OTHER OPPORTUNITIES TX- Austin Cytotech NY-Orange/Rockland County Lab Systems Administrator Of course I can?t put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Remember it never hurts to look. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From rjbuesa <@t> yahoo.com Tue Oct 12 10:16:40 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 12 10:16:45 2010 Subject: [Histonet] Defrosting Cryostat In-Reply-To: Message-ID: <131955.43108.qm@web65704.mail.ac4.yahoo.com> The best solution is to buy a "self-defrosting" cryostat. They work in the same way as your home freezer and do not build-up ice. Ren? J. --- On Tue, 10/12/10, Emily Sours wrote: From: Emily Sours Subject: Re: [Histonet] Defrosting Cryostat To: Beth.Fye@hcahealthcare.com, histonet@lists.utsouthwestern.edu Date: Tuesday, October 12, 2010, 10:31 AM Our cryostat is a CM3050--we let it defrost overnight with the glass door open and it's fine the next morning.? alternatively, you could defrost it over a weekend.? i don't know how long disinfecting takes, though, so this may not be feasible for you. also, it only takes a few hours more to get back down to the working temperature, so it's ready to use the next day, just later, at say noon or so. Emily -- Correction: This blog post originally stated that one in three black men who have sex with me is HIV positive. In fact, the statistic applies to black men who have sex with men. http://www.tbd.com/blogs/amanda-hess/2010/10/hiv-positive-black-gay-men-to-get-the-bayard-rustin-project-a-district-campaign-against-aids-2873.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Cline <@t> wchsys.org Tue Oct 12 10:10:05 2010 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Tue Oct 12 10:20:30 2010 Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Message-ID: Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Janice.Mahoney <@t> alegent.org Tue Oct 12 10:24:54 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Oct 12 10:25:02 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local> We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From sgoebel <@t> xbiotech.com Tue Oct 12 10:29:01 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Oct 12 10:29:05 2010 Subject: [Histonet] Defrosting Cryostat Message-ID: <20101012082901.9e2d9aa830e8449a2412eb1e4f2f067e.084757cbf8.wbe@email04.secureserver.net> Even with a self-defrosting cryostat, if you are regulated by CAP still have to decontaminate it. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 E Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: Re: [Histonet] Defrosting Cryostat From: Rene J Buesa <[1]rjbuesa@yahoo.c Date: Tue, October 12, 2010 8:16 am To: [2]Beth.Fye@hcahealthcare.co utsouthwestern.edu, Emily Sours <[4]talulahgosh@gmail. The best solution is to buy a "self-defrosting" cryostat. They work in the Ren? J. --- On Tue, 10/12/10, Emily Sours <[5]talulahgosh@gmail.com> wrote: From: Emily Sours <[6]talulahgosh@ Subject: Re: [Histonet] Defrosting Cryostat To: [7]Beth.Fye@hcahealthcare.co utsouthwestern.edu Date: Tuesday, October 12, 2010, 10:31 AM Our cryostat is a CM3050--we let it defrost overnight with the glass door open and it's fine the next morning. alternatively, you could defrost over a weekend. i don't know how long disinfecting takes, though, so may not be feasible for you. also, it only takes a few hours more to get back down to the working temperature, so it's ready to use the next day, just later, at say noon or< Emily -- Correction: This blog post originally stated that one in three black men who have sex with me is HIV positive. In fact, the statistic applies to black men who have sex with men. [9]http://www.tbd.com/blogs/amanda-hess/2010/10/hiv-positive-black-g ay-men-to-get-the-bayard-rustin-project-a-district-campaign-against-ai ds-28 _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouth [11]http: _______________________________________________ Histonet mailing list [12]Histonet@lists.utsouth [13]http: References 1. 3D"mailto:rjbuesa@yahoo.com" 2. 3D"mailto:Beth.Fye@hcahealthcare.com" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:talulahgosh@gmail.com" 5. 3D"mailto:talulahgosh@gmail.c 6. 3D"mailto:talulahgosh@gmail.com" 7. 3D"mailto:Beth.Fye@hcahealthcare.com" 8. 3D"mailto:histonet@lists.utsouthwestern.edu" 9. 3D"http://www.tbd.com/blogs/amanda-hess/2010/10/hiv-positive-black- 10. 3D"mailto:Histonet@lists.utsouthwestern.edu" 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 12. 3D"mailto:Histonet@lists.utsouthwestern.edu" 13. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From rjbuesa <@t> yahoo.com Tue Oct 12 10:36:21 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 12 10:36:26 2010 Subject: [Histonet] Defrosting Cryostat In-Reply-To: <20101012082901.9e2d9aa830e8449a2412eb1e4f2f067e.084757cbf8.wbe@email04.secureserver.net> Message-ID: <662152.66632.qm@web65714.mail.ac4.yahoo.com> CAP deals with disinfecting/cleaning that has to be done daily. They do not care about defrosting that was the core of the initial comment. Ren? J. --- On Tue, 10/12/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: RE: [Histonet] Defrosting Cryostat To: "Rene J Buesa" Cc: Beth.Fye@hcahealthcare.com, histonet@lists.utsouthwestern.edu, "Emily Sours" Date: Tuesday, October 12, 2010, 11:29 AM Even with a self-defrosting cryostat, if you are regulated by CAP you still have to decontaminate it. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas? 78744 (512)386-5107 -------- Original Message -------- Subject: Re: [Histonet] Defrosting Cryostat From: Rene J Buesa Date: Tue, October 12, 2010 8:16 am To: Beth.Fye@hcahealthcare.com, histonet@lists.utsouthwestern.edu, Emily Sours The best solution is to buy a "self-defrosting" cryostat. They work in the same way as your home freezer and do not build-up ice. Ren? J. --- On Tue, 10/12/10, Emily Sours wrote: From: Emily Sours Subject: Re: [Histonet] Defrosting Cryostat To: Beth.Fye@hcahealthcare.com, histonet@lists.utsouthwestern.edu Date: Tuesday, October 12, 2010, 10:31 AM Our cryostat is a CM3050--we let it defrost overnight with the glass door open and it's fine the next morning.? alternatively, you could defrost it over a weekend.? i don't know how long disinfecting takes, though, so this may not be feasible for you. also, it only takes a few hours more to get back down to the working temperature, so it's ready to use the next day, just later, at say noon or so. Emily -- Correction: This blog post originally stated that one in three black men who have sex with me is HIV positive. In fact, the statistic applies to black men who have sex with men. http://www.tbd.com/blogs/amanda-hess/2010/10/hiv-positive-black-gay-men-to-get-the-bayard-rustin-project-a-district-campaign-against-aids-2873.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Oct 12 10:59:42 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Oct 12 10:59:47 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Tue Oct 12 11:05:06 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Oct 12 11:05:51 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local> <661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From JWeems <@t> sjha.org Tue Oct 12 11:13:53 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 12 11:13:58 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local> <661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640398778201@CHEXCMS10.one.ads.che.org> And the 48 hours is critical to the patient who might qualify for a clinical trial. If it is not met the patient will not be allowed to participate. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, October 12, 2010 12:00 To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Teri.Hallada <@t> midmichigan.org Tue Oct 12 11:57:10 2010 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Tue Oct 12 11:57:26 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local> <661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994C49C@MAILSRV01.midmichigan.net> Sorry Jan, ASCO/CAP Guideline Recommendations for HER2 in Breast Cancer Article Table 6. Sample Exclusion Criteria to Perform or Interpret a HER2 FISH Assay Item #3 Tissue fixed for prolonged intervals in formalin (greater than 48 hours) Teresa Hallada MTCT (ASCP) MidMichigan Health Gratiot ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mahoney,Janice A Sent: Tue 10/12/2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. 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From Janice.Mahoney <@t> alegent.org Tue Oct 12 11:59:18 2010 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Oct 12 11:59:32 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <8839B08E3ED7364E8CBBD53882C984D50994C49C@MAILSRV01.midmichigan.net> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local> <661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> <8839B08E3ED7364E8CBBD53882C984D50994C49C@MAILSRV01.midmichigan.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D46@EXCHMBC2.ad.ah.local> Thank you. Jan From: Teri.Hallada@midmichigan.org [mailto:Teri.Hallada@midmichigan.org] Sent: Tuesday, October 12, 2010 11:57 AM To: Mahoney,Janice A; mpence@grhs.net; Joyce.Cline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Sorry Jan, ASCO/CAP Guideline Recommendations for HER2 in Breast Cancer Article Table 6. Sample Exclusion Criteria to Perform or Interpret a HER2 FISH Assay Item #3 Tissue fixed for prolonged intervals in formalin (greater than 48 hours) Teresa Hallada MTCT (ASCP) MidMichigan Health Gratiot ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mahoney,Janice A Sent: Tue 10/12/2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From NGUPTA1 <@t> hfhs.org Tue Oct 12 12:02:23 2010 From: NGUPTA1 <@t> hfhs.org (Gupta, Nilesh) Date: Tue Oct 12 12:02:35 2010 Subject: [Histonet] Optimal processing for prostate needle biopsies Message-ID: I have a few questions regrading processing of prostate needle biopsies. 1. What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3. What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From Melissa.Kuhnla <@t> chsli.org Tue Oct 12 12:02:32 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Tue Oct 12 12:02:42 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local><661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> Message-ID: I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From p_bourne_14526 <@t> yahoo.com Tue Oct 12 12:25:54 2010 From: p_bourne_14526 <@t> yahoo.com (Patricia Bourne) Date: Tue Oct 12 12:26:06 2010 Subject: [Histonet] Leica EG1150 Tissue Embedding Center In-Reply-To: References: Message-ID: <970880.88981.qm@web51907.mail.re2.yahoo.com> What is the best embedding center?? Also, is anyone using the Biocare Autostainer and if so how do you like it. Thanks ________________________________ From: "Maryott, Bridget" To: "histonet@lists.utsouthwestern.edu" Sent: Mon, October 11, 2010 6:11:24 AM Subject: [Histonet] Leica EG1150 Tissue Embedding Center Is anyone using the new Leica EG1150 tissue embedding center? Any pros/cons? Is the cold plate area sufficient? Is it that much better than the EG1160? Thank you kindly for your time, Bridget Maryott, HT (ASCP) Advanced Staining Ventana Medical Systems, Inc. a member of the Roche Group 1910 E. Innovation Park Drive Tucson, Arizona? 85755 Tel: +1 520 229 4022 mailto: bridget.maryott@ventana.roche.com Confidentiality Note: This message is intended only for the use of the named recipient(s) and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arodrigues <@t> cvm.tamu.edu Tue Oct 12 12:28:42 2010 From: arodrigues <@t> cvm.tamu.edu (Aline Rodrigues) Date: Tue Oct 12 12:28:50 2010 Subject: [Histonet] Immunofluorescence double labeling with two rabbit antibodies Message-ID: <748C42C8-ADAB-4688-8283-A35C7A436169@cvm.tamu.edu> Hello all, I using formalin fixed paraffin embedded specimens from fetuses from sheep to examine which cells are virally infected within the CNS. I am using IBA-1 to identify microglial cells. The IBA-1 antibody is a rabbit antibody and it works really well in my specimens. At the same time I am also using an antibody against the virus that I am studying that is also a rabbit Ab. For these samples I am using double labeling to determine if cells are infected or not. Both antibodies work, but I can use them together otherwise there is x- reaction. Does anyone has any suggestions of how to label tissues with two different antibodies that come from the same species. On immunoportal someone suggested to use 4%PFA to fix the tissues in between the first (primary and secondary Ab) and the second (primary and secondary Ab), however it did not work. PFA did not block the previously bound Abs. The other option that I would have would be to use IBA-1 from goat. My problem with this antibody coming from goat is that I can have x- reaction and moderate background since goat and sheep are very closely related. Please, if someone has any suggestions I would immensely appreciate! Thank you very much! I hope you have a wonderful day! Aline From RCazares <@t> schosp.org Tue Oct 12 13:17:43 2010 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Tue Oct 12 13:17:52 2010 Subject: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject In-Reply-To: <8839B08E3ED7364E8CBBD53882C984D50994C49C@MAILSRV01.midmichigan.net> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local><661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net><8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> <8839B08E3ED7364E8CBBD53882C984D50994C49C@MAILSRV01.midmichigan.net> Message-ID: <572D1F45B44B3D4096D554B4CB40639C032028C6E3@EXCHCCRMB.schosp.org> In the summer of this year, ASCO/CAP issued new guidelines for proper handling of breast specimens to improve the accuracy of ER and PR status. It states that the fixation of breast specimens must extend for at least six hours and no more than 72 hours. It stands to reason that these guidelines should also stand for Her2 testing. On the second to the last paragraph before the authors disclaimer it reads, "We are confident that these guidelines and measures developed for testing of ER, PgR, and HER2 will improve performance of laboratories using these and future predictive testing methods." You can find the guidelines at this website, click on FULL TEXT (on the right). www.asco.org/guidelines/erpr Comments? Opinions? Anyone? Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Tuesday, October 12, 2010 11:57 AM To: Janice.Mahoney@alegent.org; mpence@grhs.net; Joyce.Cline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject Sorry Jan, ASCO/CAP Guideline Recommendations for HER2 in Breast Cancer Article Table 6. Sample Exclusion Criteria to Perform or Interpret a HER2 FISH Assay Item #3 Tissue fixed for prolonged intervals in formalin (greater than 48 hours) Teresa Hallada MTCT (ASCP) MidMichigan Health Gratiot ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mahoney,Janice A Sent: Tue 10/12/2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From JWeems <@t> sjha.org Tue Oct 12 13:30:36 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 12 13:30:42 2010 Subject: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject In-Reply-To: <572D1F45B44B3D4096D554B4CB40639C032028C6E3@EXCHCCRMB.schosp.org> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local><661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net><8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> <8839B08E3ED7364E8CBBD53882C984D50994C49C@MAILSRV01.midmichigan.net> <572D1F45B44B3D4096D554B4CB40639C032028C6E3@EXCHCCRMB.schosp.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164039877827A@CHEXCMS10.one.ads.che.org> Yes, but they didn't include HER2 officially. Those guidelines are still to come is my understanding. And then there is documenting the "cold ishemic time". The nursing staff here have been really good about it. They have to document when it was removed from the body and when it was put in formalin. There is not much difference unless it goes for xray before being put in formalin. Always something new to document! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: Tuesday, October 12, 2010 14:18 To: Teri.Hallada@midmichigan.org; Janice.Mahoney@alegent.org; mpence@grhs.net; Joyce.Cline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: RE: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject In the summer of this year, ASCO/CAP issued new guidelines for proper handling of breast specimens to improve the accuracy of ER and PR status. It states that the fixation of breast specimens must extend for at least six hours and no more than 72 hours. It stands to reason that these guidelines should also stand for Her2 testing. On the second to the last paragraph before the authors disclaimer it reads, "We are confident that these guidelines and measures developed for testing of ER, PgR, and HER2 will improve performance of laboratories using these and future predictive testing methods." You can find the guidelines at this website, click on FULL TEXT (on the right). www.asco.org/guidelines/erpr Comments? Opinions? Anyone? Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Tuesday, October 12, 2010 11:57 AM To: Janice.Mahoney@alegent.org; mpence@grhs.net; Joyce.Cline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject Sorry Jan, ASCO/CAP Guideline Recommendations for HER2 in Breast Cancer Article Table 6. Sample Exclusion Criteria to Perform or Interpret a HER2 FISH Assay Item #3 Tissue fixed for prolonged intervals in formalin (greater than 48 hours) Teresa Hallada MTCT (ASCP) MidMichigan Health Gratiot ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mahoney,Janice A Sent: Tue 10/12/2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mpence <@t> grhs.net Tue Oct 12 13:30:38 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Oct 12 13:30:45 2010 Subject: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject In-Reply-To: <572D1F45B44B3D4096D554B4CB40639C032028C6E3@EXCHCCRMB.schosp.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974A51@is-e2k3.grhs.net> I do not see anything in this article that states the CAP will accept 72hr fixation for HER2. -----Original Message----- From: Cazares, Ruth [mailto:RCazares@schosp.org] Sent: Tuesday, October 12, 2010 1:18 PM To: Teri.Hallada@midmichigan.org; Janice.Mahoney@alegent.org; Mike Pence; Joyce.Cline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: RE: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject In the summer of this year, ASCO/CAP issued new guidelines for proper handling of breast specimens to improve the accuracy of ER and PR status. It states that the fixation of breast specimens must extend for at least six hours and no more than 72 hours. It stands to reason that these guidelines should also stand for Her2 testing. On the second to the last paragraph before the authors disclaimer it reads, "We are confident that these guidelines and measures developed for testing of ER, PgR, and HER2 will improve performance of laboratories using these and future predictive testing methods." You can find the guidelines at this website, click on FULL TEXT (on the right). www.asco.org/guidelines/erpr Comments? Opinions? Anyone? Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Tuesday, October 12, 2010 11:57 AM To: Janice.Mahoney@alegent.org; mpence@grhs.net; Joyce.Cline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject Sorry Jan, ASCO/CAP Guideline Recommendations for HER2 in Breast Cancer Article Table 6. Sample Exclusion Criteria to Perform or Interpret a HER2 FISH Assay Item #3 Tissue fixed for prolonged intervals in formalin (greater than 48 hours) Teresa Hallada MTCT (ASCP) MidMichigan Health Gratiot ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mahoney,Janice A Sent: Tue 10/12/2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From AGleiberman <@t> cbiolabs.com Tue Oct 12 14:22:41 2010 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue Oct 12 14:22:46 2010 Subject: [Histonet] Immunofluorescence double labeling with two rabbitantibodies In-Reply-To: <748C42C8-ADAB-4688-8283-A35C7A436169@cvm.tamu.edu> References: <748C42C8-ADAB-4688-8283-A35C7A436169@cvm.tamu.edu> Message-ID: Aline, Jackson Immunoresearch provides protocol and reagents that are useful for solving your problem: http://www.jacksonimmuno.com/technical/fab-blok.asp I have used this approach to localize at the same sample simultaneously two different isoforms of a transcription factor with two different mouse monoclonals. Worked for me. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aline Rodrigues Sent: Tuesday, October 12, 2010 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunofluorescence double labeling with two rabbitantibodies Hello all, I using formalin fixed paraffin embedded specimens from fetuses from sheep to examine which cells are virally infected within the CNS. I am using IBA-1 to identify microglial cells. The IBA-1 antibody is a rabbit antibody and it works really well in my specimens. At the same time I am also using an antibody against the virus that I am studying that is also a rabbit Ab. For these samples I am using double labeling to determine if cells are infected or not. Both antibodies work, but I can use them together otherwise there is x- reaction. Does anyone has any suggestions of how to label tissues with two different antibodies that come from the same species. On immunoportal someone suggested to use 4%PFA to fix the tissues in between the first (primary and secondary Ab) and the second (primary and secondary Ab), however it did not work. PFA did not block the previously bound Abs. The other option that I would have would be to use IBA-1 from goat. My problem with this antibody coming from goat is that I can have x- reaction and moderate background since goat and sheep are very closely related. Please, if someone has any suggestions I would immensely appreciate! Thank you very much! I hope you have a wonderful day! Aline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From rjbuesa <@t> yahoo.com Tue Oct 12 14:24:56 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 12 14:25:04 2010 Subject: [Histonet] Optimal processing for prostate needle biopsies In-Reply-To: Message-ID: <742914.19162.qm@web65708.mail.ac4.yahoo.com> Nilesh: 1- if you are fixing with NBF do not think that because the biopsies are small require less time in the fixative. An "acceptable" fixing time will be 8 hours 2- the XpressX120 should give you good results IF the biopsies are well fixed. 3- Harris is a better hematoxylin because it is regressive and can be controlled better than Harris that is progressive and will need more staining time as it gets older/weaker Ren? J. --- On Tue, 10/12/10, Gupta, Nilesh wrote: From: Gupta, Nilesh Subject: [Histonet] Optimal processing for prostate needle biopsies To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, October 12, 2010, 1:02 PM I have a few questions regrading processing of prostate needle biopsies. 1.? What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2.? Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3.? What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 12 14:24:56 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 12 14:25:08 2010 Subject: [Histonet] Optimal processing for prostate needle biopsies In-Reply-To: Message-ID: <742914.19162.qm@web65708.mail.ac4.yahoo.com> Nilesh: 1- if you are fixing with NBF do not think that because the biopsies are small require less time in the fixative. An "acceptable" fixing time will be 8 hours 2- the XpressX120 should give you good results IF the biopsies are well fixed. 3- Harris is a better hematoxylin because it is regressive and can be controlled better than Harris that is progressive and will need more staining time as it gets older/weaker Ren? J. --- On Tue, 10/12/10, Gupta, Nilesh wrote: From: Gupta, Nilesh Subject: [Histonet] Optimal processing for prostate needle biopsies To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, October 12, 2010, 1:02 PM I have a few questions regrading processing of prostate needle biopsies. 1.? What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2.? Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3.? What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Christina.Wilson <@t> leica-microsystems.com Tue Oct 12 15:10:52 2010 From: Christina.Wilson <@t> leica-microsystems.com (Christina.Wilson@leica-microsystems.com) Date: Tue Oct 12 15:10:56 2010 Subject: [Histonet] AUTO: is out of the office. (returning Thu 10/21/2010) Message-ID: I am out of the office from Tue 10/12/2010 until Thu 10/21/2010. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mills@leica-microsystems.com, for product management support or Karen Niewerth, karen.niewerth@leica-microsystems.com, for customer service support. Note: This is an automated response to your message "Histonet Digest, Vol 83, Issue 15" sent on 10/12/2010 11:06:31 AM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From macveigh <@t> usc.edu Tue Oct 12 17:07:51 2010 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Tue Oct 12 17:07:46 2010 Subject: [Histonet] Need maintenance contract in LA Message-ID: <5533A0101A3F416FBFCFB87C7A935CBC@DFS66DD1> Hi all, We have a very small University lab. We don't work with human tissue, so we don't actually need to show record of maintenance. We would like to cover our histology equipment with a maintenance contract just in case because it is mostly old. Does someone in LA area offer maintenance for histo equipment and how much would this be. Beside all the old stuff we also have a new Leica Bond, which we would like to cover with someone different then Leica. Please contact me off the list. Michelle Aloni Research Specialist macveigh@usc.edu From laurie.colbert <@t> huntingtonhospital.com Tue Oct 12 17:40:49 2010 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Oct 12 17:40:55 2010 Subject: [Histonet] Nitroblue Tetrazolium Chloride (NBTC) Message-ID: <57BE698966D5C54EAE8612E8941D768309B96087@EXCHANGE3.huntingtonhospital.com> Does anyone know where I can get this dye? It is to be used by a research doc - he plans on putting the tissue in this dye and then into 10% formalin. Laurie Colbert From pruegg <@t> ihctech.net Tue Oct 12 20:03:08 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Oct 12 20:03:52 2010 Subject: SPAM-LOW: RE: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164039877827A@CHEXCMS10.one.ads.che.org> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local><661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net><8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local><8839B08E3ED7364E8CBBD53882C984D50994C49C@MAILSRV01.midmichigan.net><572D1F45B44B3D4096D554B4CB40639C032028C6E3@EXCHCCRMB.schosp.org> <92AD9B20A6C38C4587A9FEBE3A30E164039877827A@CHEXCMS10.one.ads.che.org> Message-ID: The ASCO/CAP Guidelines for ER/Pr may indeed eventually include Her2 but that has not been published yet as far as I know so we still have to wait for the 6-72 hr fixation guidelines to be decreed for Her2, now for Her2 it is 6-48 hours. They are asking that you record cold ischemic time and time in fixation for ER/PR and it is sure to come for Her2 so if your staff are being good at providing that you are ahead of the game. Regards, Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 12, 2010 12:31 PM To: Cazares, Ruth; Teri.Hallada@midmichigan.org; Janice.Mahoney@alegent.org; mpence@grhs.net; Joyce.Cline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject Yes, but they didn't include HER2 officially. Those guidelines are still to come is my understanding. And then there is documenting the "cold ishemic time". The nursing staff here have been really good about it. They have to document when it was removed from the body and when it was put in formalin. There is not much difference unless it goes for xray before being put in formalin. Always something new to document! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: Tuesday, October 12, 2010 14:18 To: Teri.Hallada@midmichigan.org; Janice.Mahoney@alegent.org; mpence@grhs.net; Joyce.Cline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: RE: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject In the summer of this year, ASCO/CAP issued new guidelines for proper handling of breast specimens to improve the accuracy of ER and PR status. It states that the fixation of breast specimens must extend for at least six hours and no more than 72 hours. It stands to reason that these guidelines should also stand for Her2 testing. On the second to the last paragraph before the authors disclaimer it reads, "We are confident that these guidelines and measures developed for testing of ER, PgR, and HER2 will improve performance of laboratories using these and future predictive testing methods." You can find the guidelines at this website, click on FULL TEXT (on the right). www.asco.org/guidelines/erpr Comments? Opinions? Anyone? Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Tuesday, October 12, 2010 11:57 AM To: Janice.Mahoney@alegent.org; mpence@grhs.net; Joyce.Cline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject Sorry Jan, ASCO/CAP Guideline Recommendations for HER2 in Breast Cancer Article Table 6. Sample Exclusion Criteria to Perform or Interpret a HER2 FISH Assay Item #3 Tissue fixed for prolonged intervals in formalin (greater than 48 hours) Teresa Hallada MTCT (ASCP) MidMichigan Health Gratiot ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mahoney,Janice A Sent: Tue 10/12/2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___________________________________ Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Oct 12 20:25:03 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Oct 12 20:25:52 2010 Subject: SPAM-LOW: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local><661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net><8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> Message-ID: <8212B7B2807F4D43B9A6733E975DFE3D@prueggihctechlt> The problem here is that the studies have not been done or at least not published to say if 6-72 hours fixation in formalin is what we should be using for ER/PR/Her2. The ASCO/CAP Guidelines for ER/PR (not Her2) were based upon literature searches not lab data. The only reference I found regarding not extending fixation past 72 hours was from Anthony Leon in 1998, pre antigen retrieval and pre the literature search for the guidelines which limited themselves to literature published after 2000. It would be nice if the guidelines set forth by ASCO/CAP were actually based upon data tested scientifically but it is not. It is up to individual labs to do their own studies and if possible have low expression control tissue that is fixed at intervals from 6-72 hours which can be used as control indications for what ever fixation time we come across. My two sense worth. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Tuesday, October 12, 2010 11:03 AM To: Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Wed Oct 13 04:41:51 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Wed Oct 13 04:43:07 2010 Subject: [Histonet] Nitroblue Tetrazolium Chloride (NBTC) Message-ID: Laurie - Tru Sigma/Aldrich. I used to get mine from them. Cheryl From JanKeeping <@t> gmail.com Wed Oct 13 08:34:57 2010 From: JanKeeping <@t> gmail.com (JanKeeping@gmail.com) Date: Wed Oct 13 08:35:03 2010 Subject: [Histonet] Type II-A Alpha amylase derived from Bacillis species Message-ID: <90e6ba6e8ca4d16b0804927fa892@google.com> I was wondering if anyone is using this enzyme. If so, where do you buy it? Is it extremely expensive? Could you share your procedure for preparation? Janet From JanKeeping <@t> gmail.com Wed Oct 13 08:46:00 2010 From: JanKeeping <@t> gmail.com (JanKeeping@gmail.com) Date: Wed Oct 13 08:46:04 2010 Subject: [Histonet] Alpha amylase Message-ID: <0016e6d284ea5b1fd804927fd074@google.com> Sorry, I was referring to glycogen digestion and PAS staining. From Jackie.Fleming <@t> allina.com Wed Oct 13 08:54:05 2010 From: Jackie.Fleming <@t> allina.com (Fleming, Jackie M) Date: Wed Oct 13 08:54:16 2010 Subject: [Histonet] Amputation transport Message-ID: Does anyone have a transport/ carrier for transporting amputations.? We have specimens funneled into one site for grossing, and need to transport amputations AK and BK. Thanks for any help! This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. From Kim.Donadio <@t> bhcpns.org Wed Oct 13 09:09:32 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Oct 13 09:09:46 2010 Subject: [Histonet] Position opening up in Pensacola In-Reply-To: <153314.62011.qm@web65702.mail.ac4.yahoo.com> Message-ID: Hi Histonetters! We have someone retiring soon and will have a position for a histotech coming up. We do about 12,000 cases a year( 50,000 or so blocks). We have a Benchmark XT IHC, Dako special stainer and we are in the middle of getting a Vantage tracking system. We are like family here, close and care about each other. We work to tend to our patients and we also laugh together ( usually at me, but that's ok, I can hold my own :-) If any of you are interested reply, call or send me a resume. Thanks. Have a great week! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From liz <@t> premierlab.com Wed Oct 13 09:10:52 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Oct 13 09:12:46 2010 Subject: [Histonet] Amputation transport Message-ID: <002401cb6ae1$0329a3bc$0600a8c0@PremierLab.local> Try mopec -----Original Message----- From: Fleming, Jackie M Sent: Wednesday, October 13, 2010 7:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amputation transport Does anyone have a transport/ carrier for transporting amputations.? We have specimens funneled into one site for grossing, and need to transport amputations AK and BK. Thanks for any help! This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Wed Oct 13 09:15:22 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Oct 13 09:15:42 2010 Subject: [Histonet] RE: Amputation transport In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1394E0D7B8@NADCWPMSGCMS03.hca.corpad.net> Good Morning to All, We have an off site hospital and the courier has a large deep plastic storage bin, like you get at Walmart, that he brings the amputations in. It is a blue bin (of course, not clear). It works very well. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M Sent: Wednesday, October 13, 2010 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amputation transport Does anyone have a transport/ carrier for transporting amputations.? We have specimens funneled into one site for grossing, and need to transport amputations AK and BK. Thanks for any help! This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Wed Oct 13 09:32:29 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Oct 13 09:32:39 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local><661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> Message-ID: <944721.35254.qm@web43515.mail.sp1.yahoo.com> ?We run a weekend (Friday til Monday AM) ?breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree.? Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time.? We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing?? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time.? Does 70% affect antigenicity for either Her2 or ER/PR?? Any information or suggestions will be greatly appreciated.? Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.? Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.? Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail? and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Wed Oct 13 09:37:11 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Oct 13 09:37:16 2010 Subject: [Histonet] Optimal processing for prostate needle biopsies In-Reply-To: References: Message-ID: <760058.38095.qm@web43509.mail.sp1.yahoo.com> How?long are you pre-processing the prostate biopsies before processing?on the XPress120? We use the XPress50 we?fix the biopsies in Hollandes for 2 hours, then wash for 20 minutes, pre-processing solution for 10 minutes then process. We use Harris Hematoxylin?in our H&E and morphology is good. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Gupta, Nilesh" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, October 12, 2010 12:02:23 PM Subject: [Histonet] Optimal processing for prostate needle biopsies I have a few questions regrading processing of prostate needle biopsies. 1.? What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2.? Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3.? What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Oct 13 09:39:21 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 13 09:39:28 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <944721.35254.qm@web43515.mail.sp1.yahoo.com> Message-ID: <159385.56883.qm@web65703.mail.ac4.yahoo.com> Phyllis: The time required in NBF is not 8 hours only, that is exactly what the regulations are trying to avoid, i.e. incomplete fixation. Ren??J. --- On Wed, 10/13/10, Phyllis Thaxton wrote: From: Phyllis Thaxton Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Mahoney,Janice A" , "Mike Pence" , "Joyce Cline" , histonet@lists.utsouthwestern.edu Date: Wednesday, October 13, 2010, 10:32 AM ?We run a weekend (Friday til Monday AM) ?breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree.? Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time.? We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing?? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time.? Does 70% affect antigenicity for either Her2 or ER/PR?? Any information or suggestions will be greatly appreciated.? Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.? Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.? Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail? and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Wed Oct 13 09:39:46 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Oct 13 09:39:51 2010 Subject: [Histonet] FW: Joint Commission seeks NSH Member Response Message-ID: <73A7ED895EE0C24D9267ED814911DF191773F76E@exchange.cmc-nh.org> Hey Histonetters. How about all of us getting involved in providing some input to the Joint Commission on their accreditation standards with regard to Histopathology. Apparently, there has not been a great response from the histology community to this request. This is our opportunity to impact the way we are looked at by this accreditation agency. Thanks, Steve ________________________________ From: Carrie Diamond [mailto:carrie@nsh.org] Sent: Tuesday, October 12, 2010 9:28 PM To: Feher, Stephen Subject: Joint Commission seeks NSH Member Response Dear Stephen: The Joint Commission is requesting your response to the new standards' revisions, especially in the area of histopathology. The Joint Commission Laboratory Accreditation Program recently revised the existing laboratory accreditation standards and developed new standards in several specialty areas. These changes were made in response to feedback from customer surveys conducted in the fall of 2009, as well as an expert panel review in collaboration with the ASCP. More information on these revisions and the field review response process, please visit: http://www.jointcommission.org/Standards/FieldReviews/ The changes in these standards will directly impact the day to day activities for Joint Commission accredited laboratories. Participation in this survey gives you the opportunity to have an influence on your accreditation standards and elements of performance. The survey was opened on Sept. 8, 2010 and will be available until October 20, 2010. At this time, the Joint Commission has received very few responses for the histopathology section, so we are asking for your help to spread the word and to gather responses for the survey. Thank you, Carrie Diamond, Executive Director On behalf of the Board of Directors From Bill.Tench <@t> pph.org Wed Oct 13 09:42:58 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Oct 13 09:43:06 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <944721.35254.qm@web43515.mail.sp1.yahoo.com> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local><661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net><8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> <944721.35254.qm@web43515.mail.sp1.yahoo.com> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56B4@MAIL1.pph.local> Have you validated this processing? Leaving the tissue in 70% alcohol for 48 hours is not standard, and thus requires all of the hassles associated with validation. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Wednesday, October 13, 2010 7:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR ?We run a weekend (Friday til Monday AM) ?breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree.? Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time.? We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing?? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time.? Does 70% affect antigenicity for either Her2 or ER/PR?? Any information or suggestions will be greatly appreciated.? Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.? Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees.? Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail? and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From Melissa.Kuhnla <@t> chsli.org Wed Oct 13 09:45:04 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Oct 13 09:45:20 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <944721.35254.qm@web43515.mail.sp1.yahoo.com> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local><661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local> <944721.35254.qm@web43515.mail.sp1.yahoo.com> Message-ID: For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From FMonson <@t> wcupa.edu Wed Oct 13 10:00:55 2010 From: FMonson <@t> wcupa.edu (Monson, Frederick) Date: Wed Oct 13 10:01:03 2010 Subject: [Histonet] RE: Nitroblue Tetrazolium Chloride (NBTC) In-Reply-To: <57BE698966D5C54EAE8612E8941D768309B96087@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768309B96087@EXCHANGE3.huntingtonhospital.com> Message-ID: <86FEE8BCF5652949A08D1EB0545EB424075379C279@WCU-EX-EMP1-MB.PASSHE.LCL> Just Google: "nitroblue tetraqzonium chloride". Lots of sources. Cheers, Fred Monson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, October 12, 2010 6:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nitroblue Tetrazolium Chloride (NBTC) Does anyone know where I can get this dye? It is to be used by a research doc - he plans on putting the tissue in this dye and then into 10% formalin. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Oct 13 10:00:55 2010 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Oct 13 10:01:06 2010 Subject: [Histonet] Formalin Fixation - HER2 testing Message-ID: <4CB59167.7400.0077.1@harthosp.org> Hopefully, ASCO and CAP will relax their fixation guidelines for HER2 testing in the near future to reflect that prolonged fixation in formalin has little, if any, affect on HER2 protein overexpression (my personal experience). Another article (see below) just appeared in the October issue of the American Journal of Clinical Pathology that supports this observation. Please keep in mind that making sure that the tissue doesn't dry out, minimizing the "cold" ischemic time to under 1 hour, and submitting "thin" (2-3 mm) sections for histological processing are probably more important than time in formalin. Ibarra JA and Rogers LW: Fixation time does not affect expression of HER2/neu. Am J Clin Pathol 2010;134:594-596. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From mpence <@t> grhs.net Wed Oct 13 10:04:04 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Oct 13 10:04:14 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974A52@is-e2k3.grhs.net> How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than "normal processing" time in 70% (ie. more than 1 hr). -----Original Message----- From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From JEllin <@t> yumaregional.org Wed Oct 13 10:05:27 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Oct 13 10:06:48 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D44@EXCHMBC2.ad.ah.local><661949901A768E4F9CC16D8AF8F2838C03974A4E@is-e2k3.grhs.net><8F0EE4144E8E2F4CA1F6B051A2E5BFEE02B5354D45@EXCHMBC2.ad.ah.local><944721.35254.qm@web43515.mail.sp1.yahoo.com> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.local> OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, October 13, 2010 7:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Bill.Tench <@t> pph.org Wed Oct 13 10:12:01 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Oct 13 10:12:09 2010 Subject: [Histonet] relaxation of her2 standards Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56B8@MAIL1.pph.local> Don't hold your breath. I have had multiple conversations with at least one of the primary individuals who set the standards and i have not detected ANY willingness to modify the standards and that includes a hard and fast insistence on good validation for ANYTHING that differs from standard processing. If you are not in compliance you are asking for a world of hurt (which may extend to the patient). Of course, your other comments about the tissue management are right on. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From Timothy.Morken <@t> ucsfmedctr.org Wed Oct 13 10:15:13 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Oct 13 10:15:24 2010 Subject: [Histonet] Formalin Fixation - HER2 testing In-Reply-To: <4CB59167.7400.0077.1@harthosp.org> References: <4CB59167.7400.0077.1@harthosp.org> Message-ID: <1AAF670737F193429070841C6B2ADD4C026967E878@EXMBMCB15.ucsfmedicalcenter.org> Just a cautionary note about the article mentioned by Richard: The study only tested known 3+ samples. Any validation of fixation time reduction or extension would need to test the process on the expected range of expressions " Ibarra JA and Rogers LW: Fixation time does not affect expression of HER2/neu. Am J Clin Pathol 2010;134:594-596." Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, October 13, 2010 8:01 AM To: Histonet Cc: Hadi Yaziji; Richard Eisen, M.D. Subject: [Histonet] Formalin Fixation - HER2 testing Hopefully, ASCO and CAP will relax their fixation guidelines for HER2 testing in the near future to reflect that prolonged fixation in formalin has little, if any, affect on HER2 protein overexpression (my personal experience). Another article (see below) just appeared in the October issue of the American Journal of Clinical Pathology that supports this observation. Please keep in mind that making sure that the tissue doesn't dry out, minimizing the "cold" ischemic time to under 1 hour, and submitting "thin" (2-3 mm) sections for histological processing are probably more important than time in formalin. Ibarra JA and Rogers LW: Fixation time does not affect expression of HER2/neu. Am J Clin Pathol 2010;134:594-596. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Oct 13 10:23:55 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Oct 13 10:23:59 2010 Subject: [Histonet] Type II-A Alpha amylase derived from Bacillis species In-Reply-To: <90e6ba6e8ca4d16b0804927fa892@google.com> References: <90e6ba6e8ca4d16b0804927fa892@google.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164039877841E@CHEXCMS10.one.ads.che.org> Sigma-Aldrich has it.j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JanKeeping@gmail.com Sent: Wednesday, October 13, 2010 09:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Type II-A Alpha amylase derived from Bacillis species I was wondering if anyone is using this enzyme. If so, where do you buy it? Is it extremely expensive? Could you share your procedure for preparation? Janet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From JWeems <@t> sjha.org Wed Oct 13 10:41:17 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Oct 13 10:41:25 2010 Subject: [Histonet] Bladder Diverticuli Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164039877842B@CHEXCMS10.one.ads.che.org> If TUR is 88307 and resection is 88309, what CPT code do you use for bladder diverticuli? Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From dchihc <@t> yahoo.com Wed Oct 13 10:58:31 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Oct 13 10:58:37 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <159385.56883.qm@web65703.mail.ac4.yahoo.com> References: <159385.56883.qm@web65703.mail.ac4.yahoo.com> Message-ID: <744248.34579.qm@web43513.mail.sp1.yahoo.com> Rene, ?8 hours is what is on the processor. We keep a log book on all breast tissue. The OR nurse documents on the label; ?the time of excision (placed in 10% NBF)?. The gross assistant documents the time of dissection, Then the time the tissues are placed on the processor.?Our fixation times?for breast average about 12?to 14 hours.?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: Rene J Buesa To: MelissaKuhnla ; Janice AMahoney ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu; Phyllis Thaxton Sent: Wed, October 13, 2010 9:39:21 AM Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Phyllis: The time required in NBF is not 8 hours only, that is exactly what the regulations are trying to avoid, i.e. incomplete fixation. Ren??J. --- On Wed, 10/13/10, Phyllis Thaxton wrote: >From: Phyllis Thaxton >Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR >To: "Kuhnla, Melissa" , "Mahoney,Janice A" >, "Mike Pence" , "Joyce Cline" >, histonet@lists.utsouthwestern.edu >Date: Wednesday, October 13, 2010, 10:32 AM > > > >?We run a weekend (Friday til Monday AM) ?breast run where the tissues are in >10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete >processing on Monday morning. So far no problems. >Phyllis Thaxton HT(ASCP)QIHC >DCH Regional Medical Center >Tuscaloosa, AL > > > > >________________________________ >From: "Kuhnla, Melissa" >To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu >Sent: Tue, October 12, 2010 12:02:32 PM >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > >I disagree.? Prolonged formalin fixation (over 48 hrs), diminishes >signals > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Mahoney,Janice A >Sent: Tuesday, October 12, 2010 12:05 PM >To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > >Formalin fixation time does not impact the results of FISH as it does >IHC. >Jan M >Omaha > >-----Original Message----- >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Tuesday, October 12, 2010 11:00 AM >To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > >I don't think it matters if you do Her2 by FISH or IHC the time is still >48hr. I hope I am wrong, but I don't think I am. > >Mike > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Mahoney,Janice A >Sent: Tuesday, October 12, 2010 10:25 AM >To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu >Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > >We have decided to reflex to FISH those breasts that do not fall within >the recommended formalin fixation time.? We do work on Saturdays so it >is only the rare 3 day weekends that this comes into play. Jan M Omaha > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce >Cline >Sent: Tuesday, October 12, 2010 10:10 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > >Does anyone have any experience with storing formalin fixed breast >tissue in 70% before processing?? I am trying to comply with the new >guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and >since my lab does not operate on the weekend we have been well above the >48 hour recommended formalin fixation time.? Does 70% affect >antigenicity for either Her2 or ER/PR?? Any information or suggestions >will be greatly appreciated.? Thanks :) > > >Ronda Souders >Hagerstown Medical Laboratory >301-665-4980 >fax 301-665-4941 >ronda.souders@wchsys.org > >________________________________ >***** CONFIDENTIALITY NOTICE ***** This message contains confidential >information and is intended only for the individual named. If you are >not the named addressee you should not disseminate, distribute or copy >this e-mail. Please notify the sender immediately by e-mail if you have >received this e-mail by mistake and delete this e-mail from your system. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is >faithful to the healing ministry of Jesus Christ, providing high quality >care for the body, mind and spirit of every person. > >The information contained in this communication, including attachments, >is confidential and private and intended only for the use of the >addressees.? Unauthorized use, disclosure, distribution or copying is >strictly prohibited and may be unlawful.? If you received this >communication in error, please inform us of the erroneous delivery by >return e-mail message from your computer.? Additionally, although all >attachments have been scanned at the source for viruses, the recipient >should check any attachments for the presence of viruses before opening. >Alegent Health accepts no liability for any damage caused by any virus >transmitted by this e-mail.? Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is >faithful to the healing ministry of Jesus Christ, providing high quality >care for the body, mind and spirit of every person. > >The information contained in this communication, including attachments, >is confidential and private and intended only for the use of the >addressees.? Unauthorized use, disclosure, distribution or copying is >strictly prohibited and may be unlawful.? If you received this >communication in error, please inform us of the erroneous delivery by >return e-mail message from your computer.? Additionally, although all >attachments have been scanned at the source for viruses, the recipient >should check any attachments for the presence of viruses before opening. >Alegent Health accepts no liability for any damage caused by any virus >transmitted by this e-mail.? Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >The information in this e-mail, and any attachments therein, is confidential and > >for use by the intended addressee only. If this message is received by you in >error please do not disseminate or read further. Please reply to the sender that > >you have received the message in error, then delete the message. Although >Catholic Health Services of Long Island attempts to sweep e-mail? and >attachments for viruses, it does not guarantee that either are virus-free and >accepts no liability for any damage sustained as a result of viruses. Thank you. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Oct 13 10:59:15 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Oct 13 10:59:20 2010 Subject: [Histonet] Hepatitis C Immuno staining in FFPE Liver Message-ID: Hi Histonet, I have been trying to work up HCV antibody without much success. Anyone out there successfully doing this that would like to share their protocol with me? I have many cases that have been diagnosed with HCV infection, so the control tissue is not an issue. We are using the Dako Flex Detection. Thank you in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From ktuttle <@t> umm.edu Wed Oct 13 11:07:37 2010 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed Oct 13 11:07:42 2010 Subject: [Histonet] IHC OOps wrong secondary In-Reply-To: References: Message-ID: <4CB5A11B.90CE.001A.3@umm.edu> Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From BoozerKA <@t> ah.org Wed Oct 13 11:10:54 2010 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Wed Oct 13 11:11:07 2010 Subject: [Histonet] Amputation transport In-Reply-To: <002401cb6ae1$0329a3bc$0600a8c0@PremierLab.local> References: <002401cb6ae1$0329a3bc$0600a8c0@PremierLab.local> Message-ID: <4CB5779D.4AA8.00C0.1@ah.org> If you go to a general store like Fred Meyers, Target...you can get the plastic containers that hold wrapping paper rolls that are opaque and put appropriate stickers on them for less than $20.00 I have been using 3 of them for years now. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 1-503-251-6266 ex. 10246 boozerka@ah.org >>> "Liz Chlipala" 10/13/2010 7:10 AM >>> Try mopec -----Original Message----- From: Fleming, Jackie M Sent: Wednesday, October 13, 2010 7:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amputation transport Does anyone have a transport/ carrier for transporting amputations.? We have specimens funneled into one site for grossing, and need to transport amputations AK and BK. Thanks for any help! This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Oct 13 11:17:11 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 13 11:17:16 2010 Subject: [Histonet] IHC OOps wrong secondary In-Reply-To: <4CB5A11B.90CE.001A.3@umm.edu> Message-ID: <558540.41540.qm@web65707.mail.ac4.yahoo.com> Yes Ren? J. --- On Wed, 10/13/10, Kimberly Tuttle wrote: From: Kimberly Tuttle Subject: [Histonet] IHC OOps wrong secondary To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, October 13, 2010, 12:07 PM Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle? HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Oct 13 11:42:16 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Oct 13 11:43:26 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.local> Message-ID: <1E0A9694A88D47B0A2E14496A7947381@lurie.northwestern.edu> All, I've been watching this thread for a couple of days now and would like all to know that had they attended workshop 17, provided they were in Seattle, it would have been most insightful regarding all these guidelines. It was titled "Quality Biospecimens for Personalized Molecular Healthacare" It addressed fixation as well as cold ischemia time. Dr. Robb also commented that as a governor for CAP,there will be CAP guidelines for tissue banking and procurement at some point in time. I only have the handout but James Robb, the speaker, would have is e-mail listed in the program we received in Seattle (it's at home, not here at work) I can look it up or if someone has it handy, please chime in for those interested. Unofortunately, this was an underpopulated workshop, but very informative. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Wednesday, October 13, 2010 10:05 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, October 13, 2010 7:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed Oct 13 12:06:50 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Oct 13 12:06:55 2010 Subject: [Histonet] pathology reports Message-ID: <25A4DE08332B19499904459F00AAACB7181249D05B@EVS1.archildrens.org> When you have results from an outside lab, i.e. flow results, bone marrows, ect. How do you integrate this into your path report? It is a verbatim copy inside the report in the same format as the outside lab? Or can it be a copy with the results in a different format? A discussion has occurred within in or transcription department over this matter. We do attach the outside report to the hard copy report in our paper file. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From MLashus <@t> pathgroup.com Wed Oct 13 12:21:05 2010 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Wed Oct 13 12:21:09 2010 Subject: [Histonet] Seeking Grossing/Histo Tech Message-ID: <197CD0B02A81F94994A285C59C8AE05C05F49D4CDB@pgnexchange.pathgroup.com> We are seeking a qualified candidate for the position of Grossing/Lead Histo Tech in our Chattanooga location. We are a state of art laboratory using the Ventana Vantage system to maintain specimen integrity; we also have Ventana special stainers and the Benchmark Ultra and XTs for our Immunohistochemistry stains. We offer a friendly working environment along with an excellent benefit package. You may obtain more information about this position at CareerBuilders.com. We also have an opening for a cytotechnologist at our Pathology office on the Erlanger Health System campus in Chattanooga, TN. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From rsrichmond <@t> gmail.com Wed Oct 13 12:26:33 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Oct 13 12:26:40 2010 Subject: [Histonet] Re: Urinary bladder diverticula Message-ID: Joyce Weems in Atlanta asks: >>If TUR is 88307 and resection is 88309, what CPT code do you use for bladder diverticuli?<< A transurethral resection of the prostate (TURP) is coded 88305 no matter how many blocks. A TUR of the bladder (presumably a TURBT, that is, a TUR of a bladder tumor) is 88307. A cystectomy specimen for cancer (resection) is 88309. There are no specific instructions for a diverticulum of the urinary bladder. Diverticula of the GI tract are however coded 88305, and I would code a urinary tract diverticulum (bladder or urethra) as 88305 also. The singular is diverticulum, the plural is diverticula. There is no such word as *diverticuli. If you don't have a copy of the anatomic pathology CPT codes, 2010 edition, I can send you a PDF of a scan of those pages. Bob Richmond Samurai Pathologist Knoxville TN From Bill.Tench <@t> pph.org Wed Oct 13 12:30:24 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Oct 13 12:30:33 2010 Subject: [Histonet] pathology reports Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56C2@MAIL1.pph.local> We scan all outside laboratory reports (including consultations, flow cytometry, special immunohistochemistry, molecular tests, etc) and insert the scanned material into an addendum which is electronically tied to the primary report. If the outside report has graphic material (ie, material that cannot be converted into a "WORD" format) it has to be excised because at least our vision of Cerner Millenium will not permit non-text material to be inserted into the report document (We use an Epson scanner and Epson program that does a very handy job of converting PDF documents into WORD documents and allows for the excision of non-WORD material---like fancy headers). When appropriate, ie, it is not obvious what the results mean, we may add our own comment about how these results should be interpreted in the setting of our other material. This saves a lot of transcription (which use to be the way we accomplished this) and avoids transcriptional errors. It generally allows capture of the name and address of the outside laboratory, which is a CLIA requirement. Incorporating the results of special tests into the report is a CAP requirement. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From Cathy.Crumpton <@t> tuality.org Wed Oct 13 12:40:39 2010 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Wed Oct 13 12:40:45 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 19 In-Reply-To: <20101013170130.2413917C8CF8@tumbleweed.corp.tuality.net> References: <20101013170130.2413917C8CF8@tumbleweed.corp.tuality.net> Message-ID: We are not open on weekends and worked out a deal with our core l that is open 24/7. When we have a breast on the processer we will run a Sat. program that ends at 21:00. They added a task on their dai They j were being embed the embedding cent embedding. No harm Cathy Tuality Community Hospital Hillsbo (503)681-1292 From Melissa.Kuhnla <@t> chsli.org Wed Oct 13 12:51:45 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Oct 13 12:52:02 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974A52@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974A52@is-e2k3.grhs.net> Message-ID: Fixation prior to the processor will not exce4ed 12 hrs. That is why we program the processor for 36...not to exceed 48. ________________________________ From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, October 13, 2010 11:04 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than "normal processing" time in 70% (ie. more than 1 hr). -----Original Message----- From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From bill501 <@t> mindspring.com Wed Oct 13 14:12:59 2010 From: bill501 <@t> mindspring.com (Bill B.) Date: Wed Oct 13 14:13:23 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C03974A52@is-e2k3.grhs.net> Message-ID: How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Oct 13 14:23:10 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Oct 13 14:25:52 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C03974A52@is-e2k3.grhs.net> , Message-ID: If our cases are needle cores they are almost immediately put into formalin from the patient. That time is recorded by the nurse or the clinician that took the specimen. The larger breast samples are received fresh from the OR and are immediately grossed either by a PA or resident. Those samples are examined, sliced (to expose the surface area of the specimen) and placed into formalin. That is when the fixation time is recorded for the larger specimens. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. [bill501@mindspring.com] Sent: Wednesday, October 13, 2010 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Wed Oct 13 16:03:28 2010 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Oct 13 16:04:07 2010 Subject: [Histonet] FW: How to remove Hematoxilin Message-ID: Hi histoworld, I would like to repeat my staining on the slides already coverslipped but need to remove hematoxilin first. How to remove hematoxilin? Is it need to repeat Antigen retrieval? Thanks in advance, Naira From ROrr <@t> northshore.org Wed Oct 13 16:29:49 2010 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Wed Oct 13 16:29:54 2010 Subject: [Histonet] I agree with Jose, etal. In-Reply-To: <7457042b-60a4-4d6c-b66c-68caa482010e@EXCHCAS02.enhnet.org> Message-ID: Thanks for your response, Jose, I agree with your points. I see these guidelines as the first attempt to standardize something that has numerous variables. How can we expect to have the manufacturers of these markers offer (or be required by the FDA) consistent results when our end of this process is "all over the board"? An ER, PR Her2 that we both buy from the same manufacturer should work the exact same way in my lab as it does in yours, IF we both process in the exact same way. I doubt very much if histology and IHC testing will ever be as exact as a clinical chemistry assay for example, but it's a start. I forsee the time gap in formalin shortening as labs get used to this first step. This would mean more validation, but since we should be doing validation each year, then this may not be such a giant task. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 From Timothy.Morken <@t> ucsfmedctr.org Wed Oct 13 17:12:54 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Wed Oct 13 17:13:03 2010 Subject: [Histonet] Training labs in the San Francisco Bay Area? Message-ID: <1AAF670737F193429070841C6B2ADD4C026967E880@EXMBMCB15.ucsfmedicalcenter.org> To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org From amosbrooks <@t> gmail.com Wed Oct 13 17:21:22 2010 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Oct 13 17:21:26 2010 Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Message-ID: Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.local > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin From JMacDonald <@t> mtsac.edu Wed Oct 13 17:30:21 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Oct 13 17:30:29 2010 Subject: [Histonet] Training labs in the San Francisco Bay Area? In-Reply-To: <1AAF670737F193429070841C6B2ADD4C026967E880@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: With the on-line programs the training facility will need to be approved by the program. They will need to sign an affiliation agreement between the program (school) and the training site. "Morken, Tim" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/13/2010 03:17 PM To Histonet cc Subject [Histonet] Training labs in the San Francisco Bay Area? To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ktuttle <@t> umm.edu Wed Oct 13 18:14:57 2010 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed Oct 13 18:15:08 2010 Subject: [Histonet] IHC OOps wrong secondary In-Reply-To: <4CB5A11B.90CE.001A.3@umm.edu> References: <4CB5A11B.90CE.001A.3@umm.edu> Message-ID: <4CB60536.90CE.001A.3@umm.edu> Thanks to everyone who responded. I removed the coverslip, and ran it back to water and re applied the secondary, dab and counterstain. It worked like a charm. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >>> "Kimberly Tuttle" 10/13/2010 12:07 pm >>> Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From histologyinfo <@t> gmail.com Wed Oct 13 19:34:17 2010 From: histologyinfo <@t> gmail.com (Pedro Louro) Date: Wed Oct 13 19:34:27 2010 Subject: [Histonet] "2010 Focus on IHC" one day event comes to NJ Message-ID: The date is set, the topics are in place, and the money is just right, all we need....is you to attend. When: Friday, November 5, 2010 Where: Somerset Medical Center 110 Rehill Ave Somerville, NJ 08876 *"2010 Focus on IHC"* presented by NJSH and sponsored by BioCare Medical, LLC $10.00(members) $40.00 (Non-members) gets you: - 6 CEU's - Breakfast, Lunch and Snacks - Great learning experience Hope to see you there, Pedro Louro President (New Jersey Society for Histotechnology) Co-chair Membership Committee ___________________________________________________________________________ *Meeting Schedule * 7:30-8:30AM Registration and Continental Breakfast * 8:30-12:00 *AM Session with Coffee Break * Seminars * Mouse Models and IHC; Linda Dean Antibodies 2010; Fatima Natar * OR ** Wet Workshop * Multiplex Staining in the Anatomic Laboratory; Tara Kennedy * 12:00-1:00 *Lunch (wraps, salad, soup, dessert) * 1:00-4:30 *PM Session with Coffee Break * Seminars * CAP Regulations; Terry Murphy Validation in the IHC Laboratory; George Hoernig * OR ** Wet Workshop * Rapid In Situ Hybridization; Will Chappell From A.Salih <@t> uws.edu.au Wed Oct 13 23:34:27 2010 From: A.Salih <@t> uws.edu.au (Anya Salih) Date: Wed Oct 13 23:34:34 2010 Subject: [Histonet] Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. Message-ID: <512D5A4F81BF054F9735F9C0AC45AB3B0281BC51@VIOLA.AD.UWS.EDU.AU> > You and your students are invited to attend the 3rd Advanced > Bio-Imaging Workshop at the University of Western Sydney on 16 - 19 > November, 2010 and the Bio-Imaging Expo (free event on 16th November > 2010) > > Tracking Molecules with Light > > Training in confocal imaging and protein 3D tracking, aggregation, > diffusion analyses. Experiments will involve mammalian cell lines, > invertebrate (coral) cells, plant and algae, bacteria and other > samples. > > Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop. > > Places limited to 35 and only 20 places left so register now. > > > Location: Confocal Bio-Imaging Facility, Building S8, Hawkesbury > Campus, University of Western Sydney > Organiser: Dr Anya Salih > Training by: Dr Salih UWS; Prof. E. Gratton and Dr M. Digman Univ. > California; Prof. Guy Cox Uni Sydney; Dr Wolfgang Becker, Becker & > Hickl GmbH Germany; Dr C Thoni Leica Microsystems, G. Symonds Zeiss) > > > Lectures and intensive hands-on training on confocal microscopes by > top researchers in the field. Learn how to explore and analyse the 3D > structural complexity of invertebrate, animal & plant cells, tissues > and micro-organisms, visualize and analyse movement of organelles and > molecules. Trial a range of novel GFP-type protein constructs. Discuss > your experiments and trial new approaches. Workshop emphasis on > advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B, > FLIM, photoactivatable fluorescent proteins. > > Invited speakers > > * Professor Enrico Gratton, Director Laboratory for Fluorescence > Dynamics, University of California, Irvine > * Professor Takeharu Nagai, Laboratory for Nanosystems > Physiology & Nikon Imaging Center, Hokkaido University Research > Institute Electronic Science, Japan > * Dr. Michelle Digman, Director Optical Biology Core Facility, > University of California, Irvine > * A/Professor Guy Cox, Australian Centre for Microscopy & > Microanalysis, University of Sydney > * Professor Leann Tilley, Department of Biochemistry, D/Director > Centre of Excellence in coherent X-ray Science, La Trobe University > * Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging > Facility, Garvan Institute of Medical Research, University of Sydney > * Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, > University of Sydney > * Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin > > Training sessions cover the following: > > Multi-colour Fluorescent proteins > Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby, > pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B > histone, actin, tubulin, Golgi, membrane); novel Photoactive > Fluorescent Proteins (EosFP, AmilRFP, kindling proteins, Phamret) from > reef corals. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein > localization & diffusion. > > Confocal Spectral Imaging > Acquisition of microspectral data (x, y, lambda) in 3D image stacks > from samples with multiple fluorescent probes or from fluorescent > coral tissues expressing a variety of GFP-type proteins (A. Salih > fluorescent corals > http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=he > alth)- Spectral unmixing, analysis, spectral FRET. > > Analysis of molecular movement and diffusion > Track proteins and other molecules in live cells. Measure protein > femtoliter concentrations. Monitor mobility and binding using > fluorescence correlation spectroscopy (FCS), scanning FCS, raster > image correlation spectroscopy (RICS), number & brightness (N&B) and > photon counting histogram (PCH). > > Fluorescence Lifetime Imaging Microscopy (FLIM) > - a powerful tool to analyse spatial distribution of excited state > lifetimes in samples: studied examples will include FRET-FLIM to study > protein interactions (e.g., DNA-Protein) in cells, imaging of > photoactivatable FPs, quenching of chlorophyll in plants, etc. > Hands-on training in FLIM and Phasor FLIM. > > > Workshop microscopes & companies > > Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto > optical beam splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B > Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging, > photon counting, FCS Nikon A1, Coherent Scientific Pty. Ltd - rapid > image acquisition, resonant scanner & 32 channel microspectral > detection Olymus FluoView FV1000 - variable barrier filter (VBF), > spectral detection, RICS and N&B > Ultra VIEW VoX spinning disc confocal microscope, high speed, > multichannel, 2D and 3D, FRAP, FLIP, photoactivation experiments, > PerkinElmer. > Confocal FLIM system, Becker & Hickl GmbH, Berlin A range of new > GFP-type protein constructs of many colours (cyan to far red) linked > to a variety of cellular proteins for in vivo protein localization > and dynamic studies, Evrogen And many more other instruments. > > > Registration > > Full workshop registration (lectures + training) will be limited to 35 > participants. > Registration will be on a first come first served basis. > > Contact Anya Salih a.salih@uws.edu.au to reserve your workshop place > Students $650 (GST inclusive) > All other $850 (GST inclusive) > > > Attendance of the BioImaging Expo on 16th November does not require > registration by please rsvp Pamela McMurtry > [pamela.mcmurtry@theconferenceteam.com.au] > Accommodation details at registration website, from $260 per week per > single room at UWS College. > Bus shuttle will be available between Sydney airport and the workshop > venue at UWS campus. > > Kind regards, Anya Dr Anya Salih Confocal Bio-Imaging Facility University Western Sydney Australia > 61 2 45701452 > a.salih@uws.edu.au From ree3 <@t> leicester.ac.uk Thu Oct 14 06:07:46 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Oct 14 06:07:53 2010 Subject: [Histonet] fridge/freezer storage space Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC@EXC-MBX3.cfs.le.ac.uk> Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. From pruegg <@t> ihctech.net Thu Oct 14 07:49:54 2010 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 14 07:50:35 2010 Subject: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR In-Reply-To: References: Message-ID: <26E0799EA0D8454A86A3F86DF8A6F22D@prueggihctechlt> Here here Amos. This is why I am for using multi tissue controls that have considered a range for most of the differences in tissues, fixation and processing encountered. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, October 13, 2010 4:21 PM To: JEllin@yumaregional.org; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.local > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> sanfordburnham.org Thu Oct 14 07:58:25 2010 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Thu Oct 14 07:58:32 2010 Subject: [Histonet] RE: fridge/freezer storage space In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC@EXC-MBX3.cfs.le.ac.uk> Message-ID: Hi Richard, We are a research facility and have the same issue with not always knowing where and whose stuff is in the freezer or refrigerators. Even with the best system though you still have to have people who communicate that they are taking stuff out and also replacing back where it was removed from. We have instituted the use of a system called Freezer Pro. Here is the web address http://www.ruro.com/products/freezerpro.html At least this is a start, hope it helps. Kind Regards! ? John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Thursday, October 14, 2010 7:08 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] fridge/freezer storage space Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NGUPTA1 <@t> hfhs.org Thu Oct 14 08:08:17 2010 From: NGUPTA1 <@t> hfhs.org (Gupta, Nilesh) Date: Thu Oct 14 08:13:11 2010 Subject: [Histonet] Optimal processing for prostate needle biopsies In-Reply-To: <760058.38095.qm@web43509.mail.sp1.yahoo.com> References: , <760058.38095.qm@web43509.mail.sp1.yahoo.com> Message-ID: Phyllis, We hold all our needle cores overnight and pre-process for 15 min. The problems we are having is shrunken nuclei and nucleoli are not distinct even in carcinoma cases. Cracking artifacts of cores (longitudinal), darker and paler staining along the length of the cores. We tried Harris but staining with Mayer's brings out nucleoli better. ________________________________ From: Phyllis Thaxton [dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:37 AM To: Gupta, Nilesh; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Optimal processing for prostate needle biopsies How long are you pre-processing the prostate biopsies before processing on the XPress120? We use the XPress50 we fix the biopsies in Hollandes for 2 hours, then wash for 20 minutes, pre-processing solution for 10 minutes then process. We use Harris Hematoxylin in our H&E and morphology is good. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Gupta, Nilesh" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, October 12, 2010 12:02:23 PM Subject: [Histonet] Optimal processing for prostate needle biopsies I have a few questions regrading processing of prostate needle biopsies. 1. What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3. What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. 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If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From talulahgosh <@t> gmail.com Thu Oct 14 08:15:44 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Oct 14 08:15:52 2010 Subject: [Histonet] RE: fridge/freezer storage space In-Reply-To: References: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC@EXC-MBX3.cfs.le.ac.uk> Message-ID: If you don't want to pay $2000 for software, you should just make your own excel worksheet. Have one person be in charge of it and no one is allowed to put anything in the freezer without asking for permission. I had to clean a departmental freezer once, and it was awful. So much stuff no one knew about, so I just chucked it. My motto is if it's not been claimed by three days, chuck it. Also, make everyone label their stuff with their PI's name. Or if you're not in research, with your lab's room number, or something like that. My other motto, if it doesn't have a PI's name on it, it goes in the trash. People were really whiny at first, but now there's no extra crap in the cold room or warm room unless it's labeled. I rule with an iron fist!! Don't cross the departmental manager!! Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Thu, Oct 14, 2010 at 8:58 AM, John Shelley wrote: > Hi Richard, > > We are a research facility and have the same issue with not always knowing > where and whose stuff is in the freezer or refrigerators. Even with the best > system though you still have to have people who communicate that they are > taking stuff out and also replacing back where it was removed from. > > We have instituted the use of a system called Freezer Pro. Here is the web > address http://www.ruro.com/products/freezerpro.html > > At least this is a start, hope it helps. > > Kind Regards! > > John J Shelley > Senior Research Associate, Histology Core Facility > Sanford-Burnham Medical Research Institute at Lake Nona > 6400 Sanger Road > Orlando, FL 32827 > Tel: (407) 745-2000 Ext.2517 > Fax: (407) 745-2001 > email: jshelley@sanfordburnham.org > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard > E. > Sent: Thursday, October 14, 2010 7:08 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] fridge/freezer storage space > > > Does anyone out there have an effective policy for monitoring the > storage of material at 4C,-20C or -80C. At the moment if any more > space is required the solution is usually to buy another fridge or > freezer, which then takes up more floor space, uses more electricity > and quickly fills as individuals use the space that has become available. > So can anyone suggest/ or has in place a system whereby the contents > of fridges/freezers can be monitored on a regular basis and old/out of > date stuff is disposed off. I expect that all you GLP laboratories have > it sorted, and I would welcome any input from you. Ours is a > university lab where no such rules/regulations seem to exist and > often when people leave they forget to throw out their stuff which can > then remain for years. > > Many thanks > > Richard Edwards > Leicester > University > U.K. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Thu Oct 14 09:16:37 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 14 09:23:23 2010 Subject: [Histonet] FW: How to remove Hematoxilin In-Reply-To: Message-ID: <626400.4559.qm@web65714.mail.ac4.yahoo.com> 1% hydrochloric acid will eliminate the hematoxylin. You do not need to repeat HIER (if the section has already gone throught it). Ren? J. --- On Wed, 10/13/10, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] FW: How to remove Hematoxilin To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, October 13, 2010, 5:03 PM Hi histoworld, I would like to repeat my staining on the slides already coverslipped but need to remove hematoxilin first. How to remove hematoxilin? Is it need to repeat Antigen retrieval? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cevanish <@t> mont-hosp.com Thu Oct 14 10:06:40 2010 From: cevanish <@t> mont-hosp.com (Chris Evanish) Date: Thu Oct 14 10:07:15 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <20101014130415337@smtp642.redcondor.net> References: <20101014130415337@smtp642.redcondor.net> Message-ID: <4CB6E43E.6034.00E2.0@mont-hosp.com> Ibarra JA and Rogers LW: Fixation time does not affect expression of HER2/neu. Am J Clin Pathol 2010;134:594-596. If you leave the tissue in the 70% for 36 hours, doesn't it cause the tissue to become extra hardened do to the alcohol? Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. >>> 10/14/2010 9:04 AM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. pathology reports (Horn, Hazel V) 2. Seeking Grossing/Histo Tech (Mighnon Lashus) 3. Re: Urinary bladder diverticula (Robert Richmond) 4. pathology reports (Tench, Bill) 5. Re: Histonet Digest, Vol 83, Issue 19 (Cathy.Crumpton@tuality.org) 6. RE: RE: New Cap Guidelines for Her2 and ER/PR (Kuhnla, Melissa) 7. RE: RE: New Cap Guidelines for Her2 and ER/PR (Bill B.) 8. RE: RE: New Cap Guidelines for Her2 and ER/PR (McMahon, Loralee A) 9. FW: How to remove Hematoxilin (Margaryan, Naira) 10. I agree with Jose, etal. (Orr, Rebecca) 11. Training labs in the San Francisco Bay Area? (Morken, Tim) 12. RE: New Cap Guidelines for Her2 and ER/PR (Amos Brooks) 13. Re: Training labs in the San Francisco Bay Area? (Jennifer MacDonald) 14. Re: IHC OOps wrong secondary (Kimberly Tuttle) 15. "2010 Focus on IHC" one day event comes to NJ (Pedro Louro) 16. Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. (Anya Salih) 17. fridge/freezer storage space (Edwards, Richard E.) 18. RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR (Patsy Ruegg) 19. RE: fridge/freezer storage space (John Shelley) ---------------------------------------------------------------------- Message: 1 Date: Wed, 13 Oct 2010 12:06:50 -0500 From: "Horn, Hazel V" Subject: [Histonet] pathology reports To: "Histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB7181249D05B@EVS1.archildrens.org> Content-Type: text/plain; charset="ISO-8859-1" When you have results from an outside lab, i.e. flow results, bone marrows, ect. How do you integrate this into your path report? It is a verbatim copy inside the report in the same format as the outside lab? Or can it be a copy with the results in a different format? A discussion has occurred within in or transcription department over this matter. We do attach the outside report to the hard copy report in our paper file. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 2 Date: Wed, 13 Oct 2010 12:21:05 -0500 From: Mighnon Lashus Subject: [Histonet] Seeking Grossing/Histo Tech To: "histonet@lists.utsouthwestern.edu" Cc: Dewayne Belew Message-ID: <197CD0B02A81F94994A285C59C8AE05C05F49D4CDB@pgnexchange.pathgroup.com> Content-Type: text/plain; charset="us-ascii" We are seeking a qualified candidate for the position of Grossing/Lead Histo Tech in our Chattanooga location. We are a state of art laboratory using the Ventana Vantage system to maintain specimen integrity; we also have Ventana special stainers and the Benchmark Ultra and XTs for our Immunohistochemistry stains. We offer a friendly working environment along with an excellent benefit package. You may obtain more information about this position at CareerBuilders.com. We also have an opening for a cytotechnologist at our Pathology office on the Erlanger Health System campus in Chattanooga, TN. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ------------------------------ Message: 3 Date: Wed, 13 Oct 2010 13:26:33 -0400 From: Robert Richmond Subject: [Histonet] Re: Urinary bladder diverticula To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Joyce Weems in Atlanta asks: >>If TUR is 88307 and resection is 88309, what CPT code do you use for bladder diverticuli?<< A transurethral resection of the prostate (TURP) is coded 88305 no matter how many blocks. A TUR of the bladder (presumably a TURBT, that is, a TUR of a bladder tumor) is 88307. A cystectomy specimen for cancer (resection) is 88309. There are no specific instructions for a diverticulum of the urinary bladder. Diverticula of the GI tract are however coded 88305, and I would code a urinary tract diverticulum (bladder or urethra) as 88305 also. The singular is diverticulum, the plural is diverticula. There is no such word as *diverticuli. If you don't have a copy of the anatomic pathology CPT codes, 2010 edition, I can send you a PDF of a scan of those pages. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Wed, 13 Oct 2010 10:30:24 -0700 From: "Tench, Bill" Subject: [Histonet] pathology reports To: Histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56C2@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii We scan all outside laboratory reports (including consultations, flow cytometry, special immunohistochemistry, molecular tests, etc) and insert the scanned material into an addendum which is electronically tied to the primary report. If the outside report has graphic material (ie, material that cannot be converted into a "WORD" format) it has to be excised because at least our vision of Cerner Millenium will not permit non-text material to be inserted into the report document (We use an Epson scanner and Epson program that does a very handy job of converting PDF documents into WORD documents and allows for the excision of non-WORD material---like fancy headers). When appropriate, ie, it is not obvious what the results mean, we may add our own comment about how these results should be interpreted in the setting of our other material. This saves a lot of transcription (which use to be the way we accomplished this) and avoids transcriptional errors. It generally allows capture of the name and address of the outside laboratory, which is a CLIA requirement. Incorporating the results of special tests into the report is a CAP requirement. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 5 Date: Wed, 13 Oct 2010 10:40:39 -0700 From: Cathy.Crumpton@tuality.org Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 19 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" We are not open on weekends and worked out a deal with our core l that is open 24/7. When we have a breast on the processer we will run a Sat. program that ends at 21:00. They added a task on their dai They j were being embed the embedding cent embedding. No harm Cathy Tuality Community Hospital Hillsbo (503)681-1292 ------------------------------ Message: 6 Date: Wed, 13 Oct 2010 13:51:45 -0400 From: "Kuhnla, Melissa" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Mike Pence , Phyllis Thaxton , "Mahoney,Janice A" , Joyce Cline , Message-ID: Content-Type: text/plain; charset="US-ASCII" Fixation prior to the processor will not exce4ed 12 hrs. That is why we program the processor for 36...not to exceed 48. ________________________________ From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, October 13, 2010 11:04 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than "normal processing" time in 70% (ie. more than 1 hr). -----Original Message----- From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. 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Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ------------------------------ Message: 7 Date: Wed, 13 Oct 2010 14:12:59 -0500 From: "Bill B." Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Message-ID: Content-Type: text/plain; charset="us-ascii" How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org ------------------------------ Message: 8 Date: Wed, 13 Oct 2010 15:23:10 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Bill B." , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" If our cases are needle cores they are almost immediately put into formalin from the patient. That time is recorded by the nurse or the clinician that took the specimen. The larger breast samples are received fresh from the OR and are immediately grossed either by a PA or resident. Those samples are examined, sliced (to expose the surface area of the specimen) and placed into formalin. That is when the fixation time is recorded for the larger specimens. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. [bill501@mindspring.com] Sent: Wednesday, October 13, 2010 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 13 Oct 2010 16:03:28 -0500 From: "Margaryan, Naira" Subject: [Histonet] FW: How to remove Hematoxilin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histoworld, I would like to repeat my staining on the slides already coverslipped but need to remove hematoxilin first. How to remove hematoxilin? Is it need to repeat Antigen retrieval? Thanks in advance, Naira ------------------------------ Message: 10 Date: Wed, 13 Oct 2010 16:29:49 -0500 From: "Orr, Rebecca" Subject: [Histonet] I agree with Jose, etal. To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Thanks for your response, Jose, I agree with your points. I see these guidelines as the first attempt to standardize something that has numerous variables. How can we expect to have the manufacturers of these markers offer (or be required by the FDA) consistent results when our end of this process is "all over the board"? An ER, PR Her2 that we both buy from the same manufacturer should work the exact same way in my lab as it does in yours, IF we both process in the exact same way. I doubt very much if histology and IHC testing will ever be as exact as a clinical chemistry assay for example, but it's a start. I forsee the time gap in formalin shortening as labs get used to this first step. This would mean more validation, but since we should be doing validation each year, then this may not be such a giant task. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ------------------------------ Message: 11 Date: Wed, 13 Oct 2010 15:12:54 -0700 From: "Morken, Tim" Subject: [Histonet] Training labs in the San Francisco Bay Area? To: Histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C026967E880@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org ------------------------------ Message: 12 Date: Wed, 13 Oct 2010 18:21:22 -0400 From: Amos Brooks Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: JEllin@yumaregional.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.local > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin ------------------------------ Message: 13 Date: Wed, 13 Oct 2010 15:30:21 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Training labs in the San Francisco Bay Area? To: "Morken, Tim" Cc: Histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" With the on-line programs the training facility will need to be approved by the program. They will need to sign an affiliation agreement between the program (school) and the training site. "Morken, Tim" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/13/2010 03:17 PM To Histonet cc Subject [Histonet] Training labs in the San Francisco Bay Area? To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 13 Oct 2010 19:14:57 -0400 From: "Kimberly Tuttle" Subject: Re: [Histonet] IHC OOps wrong secondary To: "histonet@lists.utsouthwestern.edu" , "Kimberly Tuttle" Message-ID: <4CB60536.90CE.001A.3@umm.edu> Content-Type: text/plain; charset=US-ASCII Thanks to everyone who responded. I removed the coverslip, and ran it back to water and re applied the secondary, dab and counterstain. It worked like a charm. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >>> "Kimberly Tuttle" 10/13/2010 12:07 pm >>> Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 15 Date: Wed, 13 Oct 2010 20:34:17 -0400 From: Pedro Louro Subject: [Histonet] "2010 Focus on IHC" one day event comes to NJ To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The date is set, the topics are in place, and the money is just right, all we need....is you to attend. When: Friday, November 5, 2010 Where: Somerset Medical Center 110 Rehill Ave Somerville, NJ 08876 *"2010 Focus on IHC"* presented by NJSH and sponsored by BioCare Medical, LLC $10.00(members) $40.00 (Non-members) gets you: - 6 CEU's - Breakfast, Lunch and Snacks - Great learning experience Hope to see you there, Pedro Louro President (New Jersey Society for Histotechnology) Co-chair Membership Committee ___________________________________________________________________________ *Meeting Schedule * 7:30-8:30AM Registration and Continental Breakfast * 8:30-12:00 *AM Session with Coffee Break * Seminars * Mouse Models and IHC; Linda Dean Antibodies 2010; Fatima Natar * OR ** Wet Workshop * Multiplex Staining in the Anatomic Laboratory; Tara Kennedy * 12:00-1:00 *Lunch (wraps, salad, soup, dessert) * 1:00-4:30 *PM Session with Coffee Break * Seminars * CAP Regulations; Terry Murphy Validation in the IHC Laboratory; George Hoernig * OR ** Wet Workshop * Rapid In Situ Hybridization; Will Chappell ------------------------------ Message: 16 Date: Thu, 14 Oct 2010 15:34:27 +1100 From: "Anya Salih" Subject: [Histonet] Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. To: Message-ID: <512D5A4F81BF054F9735F9C0AC45AB3B0281BC51@VIOLA.AD.UWS.EDU.AU> Content-Type: text/plain; charset="us-ascii" > You and your students are invited to attend the 3rd Advanced > Bio-Imaging Workshop at the University of Western Sydney on 16 - 19 > November, 2010 and the Bio-Imaging Expo (free event on 16th November > 2010) > > Tracking Molecules with Light > > Training in confocal imaging and protein 3D tracking, aggregation, > diffusion analyses. Experiments will involve mammalian cell lines, > invertebrate (coral) cells, plant and algae, bacteria and other > samples. > > Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop. > > Places limited to 35 and only 20 places left so register now. > > > Location: Confocal Bio-Imaging Facility, Building S8, Hawkesbury > Campus, University of Western Sydney > Organiser: Dr Anya Salih > Training by: Dr Salih UWS; Prof. E. Gratton and Dr M. Digman Univ. > California; Prof. Guy Cox Uni Sydney; Dr Wolfgang Becker, Becker & > Hickl GmbH Germany; Dr C Thoni Leica Microsystems, G. Symonds Zeiss) > > > Lectures and intensive hands-on training on confocal microscopes by > top researchers in the field. Learn how to explore and analyse the 3D > structural complexity of invertebrate, animal & plant cells, tissues > and micro-organisms, visualize and analyse movement of organelles and > molecules. Trial a range of novel GFP-type protein constructs. Discuss > your experiments and trial new approaches. Workshop emphasis on > advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B, > FLIM, photoactivatable fluorescent proteins. > > Invited speakers > > * Professor Enrico Gratton, Director Laboratory for Fluorescence > Dynamics, University of California, Irvine > * Professor Takeharu Nagai, Laboratory for Nanosystems > Physiology & Nikon Imaging Center, Hokkaido University Research > Institute Electronic Science, Japan > * Dr. Michelle Digman, Director Optical Biology Core Facility, > University of California, Irvine > * A/Professor Guy Cox, Australian Centre for Microscopy & > Microanalysis, University of Sydney > * Professor Leann Tilley, Department of Biochemistry, D/Director > Centre of Excellence in coherent X-ray Science, La Trobe University > * Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging > Facility, Garvan Institute of Medical Research, University of Sydney > * Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, > University of Sydney > * Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin > > Training sessions cover the following: > > Multi-colour Fluorescent proteins > Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby, > pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B > histone, actin, tubulin, Golgi, membrane); novel Photoactive > Fluorescent Proteins (EosFP, AmilRFP, kindling proteins, Phamret) from > reef corals. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein > localization & diffusion. > > Confocal Spectral Imaging > Acquisition of microspectral data (x, y, lambda) in 3D image stacks > from samples with multiple fluorescent probes or from fluorescent > coral tissues expressing a variety of GFP-type proteins (A. Salih > fluorescent corals > http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=he > alth)- Spectral unmixing, analysis, spectral FRET. > > Analysis of molecular movement and diffusion > Track proteins and other molecules in live cells. Measure protein > femtoliter concentrations. Monitor mobility and binding using > fluorescence correlation spectroscopy (FCS), scanning FCS, raster > image correlation spectroscopy (RICS), number & brightness (N&B) and > photon counting histogram (PCH). > > Fluorescence Lifetime Imaging Microscopy (FLIM) > - a powerful tool to analyse spatial distribution of excited state > lifetimes in samples: studied examples will include FRET-FLIM to study > protein interactions (e.g., DNA-Protein) in cells, imaging of > photoactivatable FPs, quenching of chlorophyll in plants, etc. > Hands-on training in FLIM and Phasor FLIM. > > > Workshop microscopes & companies > > Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto > optical beam splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B > Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging, > photon counting, FCS Nikon A1, Coherent Scientific Pty. Ltd - rapid > image acquisition, resonant scanner & 32 channel microspectral > detection Olymus FluoView FV1000 - variable barrier filter (VBF), > spectral detection, RICS and N&B > Ultra VIEW VoX spinning disc confocal microscope, high speed, > multichannel, 2D and 3D, FRAP, FLIP, photoactivation experiments, > PerkinElmer. > Confocal FLIM system, Becker & Hickl GmbH, Berlin A range of new > GFP-type protein constructs of many colours (cyan to far red) linked > to a variety of cellular proteins for in vivo protein localization > and dynamic studies, Evrogen And many more other instruments. > > > Registration > > Full workshop registration (lectures + training) will be limited to 35 > participants. > Registration will be on a first come first served basis. > > Contact Anya Salih a.salih@uws.edu.au to reserve your workshop place > Students $650 (GST inclusive) > All other $850 (GST inclusive) > > > Attendance of the BioImaging Expo on 16th November does not require > registration by please rsvp Pamela McMurtry > [pamela.mcmurtry@theconferenceteam.com.au] > Accommodation details at registration website, from $260 per week per > single room at UWS College. > Bus shuttle will be available between Sydney airport and the workshop > venue at UWS campus. > > Kind regards, Anya Dr Anya Salih Confocal Bio-Imaging Facility University Western Sydney Australia > 61 2 45701452 > a.salih@uws.edu.au ------------------------------ Message: 17 Date: Thu, 14 Oct 2010 12:07:46 +0100 From: "Edwards, Richard E." Subject: [Histonet] fridge/freezer storage space To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. ------------------------------ Message: 18 Date: Thu, 14 Oct 2010 06:49:54 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "'Amos Brooks'" , , Message-ID: <26E0799EA0D8454A86A3F86DF8A6F22D@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" Here here Amos. This is why I am for using multi tissue controls that have considered a range for most of the differences in tissues, fixation and processing encountered. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, October 13, 2010 4:21 PM To: JEllin@yumaregional.org; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.local > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 14 Oct 2010 08:58:25 -0400 From: John Shelley Subject: [Histonet] RE: fridge/freezer storage space To: "Edwards, Richard E." , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Richard, We are a research facility and have the same issue with not always knowing where and whose stuff is in the freezer or refrigerators. Even with the best system though you still have to have people who communicate that they are taking stuff out and also replacing back where it was removed from. We have instituted the use of a system called Freezer Pro. Here is the web address http://www.ruro.com/products/freezerpro.html At least this is a start, hope it helps. Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Thursday, October 14, 2010 7:08 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] fridge/freezer storage space Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 83, Issue 20 **************************************** From cevanish <@t> mont-hosp.com Thu Oct 14 10:10:12 2010 From: cevanish <@t> mont-hosp.com (Chris Evanish) Date: Thu Oct 14 10:10:50 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <20101014130415337@smtp642.redcondor.net> References: <20101014130415337@smtp642.redcondor.net> Message-ID: <4CB6E512.6034.00E2.0@mont-hosp.com> Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. Thanks, Chris Evanish Montgomery Hospital Norristown PA Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. >>> 10/14/2010 9:04 AM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. pathology reports (Horn, Hazel V) 2. Seeking Grossing/Histo Tech (Mighnon Lashus) 3. Re: Urinary bladder diverticula (Robert Richmond) 4. pathology reports (Tench, Bill) 5. Re: Histonet Digest, Vol 83, Issue 19 (Cathy.Crumpton@tuality.org) 6. RE: RE: New Cap Guidelines for Her2 and ER/PR (Kuhnla, Melissa) 7. RE: RE: New Cap Guidelines for Her2 and ER/PR (Bill B.) 8. RE: RE: New Cap Guidelines for Her2 and ER/PR (McMahon, Loralee A) 9. FW: How to remove Hematoxilin (Margaryan, Naira) 10. I agree with Jose, etal. (Orr, Rebecca) 11. Training labs in the San Francisco Bay Area? (Morken, Tim) 12. RE: New Cap Guidelines for Her2 and ER/PR (Amos Brooks) 13. Re: Training labs in the San Francisco Bay Area? (Jennifer MacDonald) 14. Re: IHC OOps wrong secondary (Kimberly Tuttle) 15. "2010 Focus on IHC" one day event comes to NJ (Pedro Louro) 16. Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. (Anya Salih) 17. fridge/freezer storage space (Edwards, Richard E.) 18. RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR (Patsy Ruegg) 19. RE: fridge/freezer storage space (John Shelley) ---------------------------------------------------------------------- Message: 1 Date: Wed, 13 Oct 2010 12:06:50 -0500 From: "Horn, Hazel V" Subject: [Histonet] pathology reports To: "Histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB7181249D05B@EVS1.archildrens.org> Content-Type: text/plain; charset="ISO-8859-1" When you have results from an outside lab, i.e. flow results, bone marrows, ect. How do you integrate this into your path report? It is a verbatim copy inside the report in the same format as the outside lab? Or can it be a copy with the results in a different format? A discussion has occurred within in or transcription department over this matter. We do attach the outside report to the hard copy report in our paper file. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 2 Date: Wed, 13 Oct 2010 12:21:05 -0500 From: Mighnon Lashus Subject: [Histonet] Seeking Grossing/Histo Tech To: "histonet@lists.utsouthwestern.edu" Cc: Dewayne Belew Message-ID: <197CD0B02A81F94994A285C59C8AE05C05F49D4CDB@pgnexchange.pathgroup.com> Content-Type: text/plain; charset="us-ascii" We are seeking a qualified candidate for the position of Grossing/Lead Histo Tech in our Chattanooga location. We are a state of art laboratory using the Ventana Vantage system to maintain specimen integrity; we also have Ventana special stainers and the Benchmark Ultra and XTs for our Immunohistochemistry stains. We offer a friendly working environment along with an excellent benefit package. You may obtain more information about this position at CareerBuilders.com. We also have an opening for a cytotechnologist at our Pathology office on the Erlanger Health System campus in Chattanooga, TN. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ------------------------------ Message: 3 Date: Wed, 13 Oct 2010 13:26:33 -0400 From: Robert Richmond Subject: [Histonet] Re: Urinary bladder diverticula To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Joyce Weems in Atlanta asks: >>If TUR is 88307 and resection is 88309, what CPT code do you use for bladder diverticuli?<< A transurethral resection of the prostate (TURP) is coded 88305 no matter how many blocks. A TUR of the bladder (presumably a TURBT, that is, a TUR of a bladder tumor) is 88307. A cystectomy specimen for cancer (resection) is 88309. There are no specific instructions for a diverticulum of the urinary bladder. Diverticula of the GI tract are however coded 88305, and I would code a urinary tract diverticulum (bladder or urethra) as 88305 also. The singular is diverticulum, the plural is diverticula. There is no such word as *diverticuli. If you don't have a copy of the anatomic pathology CPT codes, 2010 edition, I can send you a PDF of a scan of those pages. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Wed, 13 Oct 2010 10:30:24 -0700 From: "Tench, Bill" Subject: [Histonet] pathology reports To: Histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56C2@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii We scan all outside laboratory reports (including consultations, flow cytometry, special immunohistochemistry, molecular tests, etc) and insert the scanned material into an addendum which is electronically tied to the primary report. If the outside report has graphic material (ie, material that cannot be converted into a "WORD" format) it has to be excised because at least our vision of Cerner Millenium will not permit non-text material to be inserted into the report document (We use an Epson scanner and Epson program that does a very handy job of converting PDF documents into WORD documents and allows for the excision of non-WORD material---like fancy headers). When appropriate, ie, it is not obvious what the results mean, we may add our own comment about how these results should be interpreted in the setting of our other material. This saves a lot of transcription (which use to be the way we accomplished this) and avoids transcriptional errors. It generally allows capture of the name and address of the outside laboratory, which is a CLIA requirement. Incorporating the results of special tests into the report is a CAP requirement. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 5 Date: Wed, 13 Oct 2010 10:40:39 -0700 From: Cathy.Crumpton@tuality.org Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 19 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" We are not open on weekends and worked out a deal with our core l that is open 24/7. When we have a breast on the processer we will run a Sat. program that ends at 21:00. They added a task on their dai They j were being embed the embedding cent embedding. No harm Cathy Tuality Community Hospital Hillsbo (503)681-1292 ------------------------------ Message: 6 Date: Wed, 13 Oct 2010 13:51:45 -0400 From: "Kuhnla, Melissa" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Mike Pence , Phyllis Thaxton , "Mahoney,Janice A" , Joyce Cline , Message-ID: Content-Type: text/plain; charset="US-ASCII" Fixation prior to the processor will not exce4ed 12 hrs. That is why we program the processor for 36...not to exceed 48. ________________________________ From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, October 13, 2010 11:04 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than "normal processing" time in 70% (ie. more than 1 hr). -----Original Message----- From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ------------------------------ Message: 7 Date: Wed, 13 Oct 2010 14:12:59 -0500 From: "Bill B." Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Message-ID: Content-Type: text/plain; charset="us-ascii" How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org ------------------------------ Message: 8 Date: Wed, 13 Oct 2010 15:23:10 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Bill B." , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" If our cases are needle cores they are almost immediately put into formalin from the patient. That time is recorded by the nurse or the clinician that took the specimen. The larger breast samples are received fresh from the OR and are immediately grossed either by a PA or resident. Those samples are examined, sliced (to expose the surface area of the specimen) and placed into formalin. That is when the fixation time is recorded for the larger specimens. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. [bill501@mindspring.com] Sent: Wednesday, October 13, 2010 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 13 Oct 2010 16:03:28 -0500 From: "Margaryan, Naira" Subject: [Histonet] FW: How to remove Hematoxilin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histoworld, I would like to repeat my staining on the slides already coverslipped but need to remove hematoxilin first. How to remove hematoxilin? Is it need to repeat Antigen retrieval? Thanks in advance, Naira ------------------------------ Message: 10 Date: Wed, 13 Oct 2010 16:29:49 -0500 From: "Orr, Rebecca" Subject: [Histonet] I agree with Jose, etal. To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Thanks for your response, Jose, I agree with your points. I see these guidelines as the first attempt to standardize something that has numerous variables. How can we expect to have the manufacturers of these markers offer (or be required by the FDA) consistent results when our end of this process is "all over the board"? An ER, PR Her2 that we both buy from the same manufacturer should work the exact same way in my lab as it does in yours, IF we both process in the exact same way. I doubt very much if histology and IHC testing will ever be as exact as a clinical chemistry assay for example, but it's a start. I forsee the time gap in formalin shortening as labs get used to this first step. This would mean more validation, but since we should be doing validation each year, then this may not be such a giant task. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ------------------------------ Message: 11 Date: Wed, 13 Oct 2010 15:12:54 -0700 From: "Morken, Tim" Subject: [Histonet] Training labs in the San Francisco Bay Area? To: Histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C026967E880@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org ------------------------------ Message: 12 Date: Wed, 13 Oct 2010 18:21:22 -0400 From: Amos Brooks Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: JEllin@yumaregional.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.local > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin ------------------------------ Message: 13 Date: Wed, 13 Oct 2010 15:30:21 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Training labs in the San Francisco Bay Area? To: "Morken, Tim" Cc: Histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" With the on-line programs the training facility will need to be approved by the program. They will need to sign an affiliation agreement between the program (school) and the training site. "Morken, Tim" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/13/2010 03:17 PM To Histonet cc Subject [Histonet] Training labs in the San Francisco Bay Area? To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 13 Oct 2010 19:14:57 -0400 From: "Kimberly Tuttle" Subject: Re: [Histonet] IHC OOps wrong secondary To: "histonet@lists.utsouthwestern.edu" , "Kimberly Tuttle" Message-ID: <4CB60536.90CE.001A.3@umm.edu> Content-Type: text/plain; charset=US-ASCII Thanks to everyone who responded. I removed the coverslip, and ran it back to water and re applied the secondary, dab and counterstain. It worked like a charm. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >>> "Kimberly Tuttle" 10/13/2010 12:07 pm >>> Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 15 Date: Wed, 13 Oct 2010 20:34:17 -0400 From: Pedro Louro Subject: [Histonet] "2010 Focus on IHC" one day event comes to NJ To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The date is set, the topics are in place, and the money is just right, all we need....is you to attend. When: Friday, November 5, 2010 Where: Somerset Medical Center 110 Rehill Ave Somerville, NJ 08876 *"2010 Focus on IHC"* presented by NJSH and sponsored by BioCare Medical, LLC $10.00(members) $40.00 (Non-members) gets you: - 6 CEU's - Breakfast, Lunch and Snacks - Great learning experience Hope to see you there, Pedro Louro President (New Jersey Society for Histotechnology) Co-chair Membership Committee ___________________________________________________________________________ *Meeting Schedule * 7:30-8:30AM Registration and Continental Breakfast * 8:30-12:00 *AM Session with Coffee Break * Seminars * Mouse Models and IHC; Linda Dean Antibodies 2010; Fatima Natar * OR ** Wet Workshop * Multiplex Staining in the Anatomic Laboratory; Tara Kennedy * 12:00-1:00 *Lunch (wraps, salad, soup, dessert) * 1:00-4:30 *PM Session with Coffee Break * Seminars * CAP Regulations; Terry Murphy Validation in the IHC Laboratory; George Hoernig * OR ** Wet Workshop * Rapid In Situ Hybridization; Will Chappell ------------------------------ Message: 16 Date: Thu, 14 Oct 2010 15:34:27 +1100 From: "Anya Salih" Subject: [Histonet] Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. To: Message-ID: <512D5A4F81BF054F9735F9C0AC45AB3B0281BC51@VIOLA.AD.UWS.EDU.AU> Content-Type: text/plain; charset="us-ascii" > You and your students are invited to attend the 3rd Advanced > Bio-Imaging Workshop at the University of Western Sydney on 16 - 19 > November, 2010 and the Bio-Imaging Expo (free event on 16th November > 2010) > > Tracking Molecules with Light > > Training in confocal imaging and protein 3D tracking, aggregation, > diffusion analyses. Experiments will involve mammalian cell lines, > invertebrate (coral) cells, plant and algae, bacteria and other > samples. > > Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop. > > Places limited to 35 and only 20 places left so register now. > > > Location: Confocal Bio-Imaging Facility, Building S8, Hawkesbury > Campus, University of Western Sydney > Organiser: Dr Anya Salih > Training by: Dr Salih UWS; Prof. E. Gratton and Dr M. Digman Univ. > California; Prof. Guy Cox Uni Sydney; Dr Wolfgang Becker, Becker & > Hickl GmbH Germany; Dr C Thoni Leica Microsystems, G. Symonds Zeiss) > > > Lectures and intensive hands-on training on confocal microscopes by > top researchers in the field. Learn how to explore and analyse the 3D > structural complexity of invertebrate, animal & plant cells, tissues > and micro-organisms, visualize and analyse movement of organelles and > molecules. Trial a range of novel GFP-type protein constructs. Discuss > your experiments and trial new approaches. Workshop emphasis on > advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B, > FLIM, photoactivatable fluorescent proteins. > > Invited speakers > > * Professor Enrico Gratton, Director Laboratory for Fluorescence > Dynamics, University of California, Irvine > * Professor Takeharu Nagai, Laboratory for Nanosystems > Physiology & Nikon Imaging Center, Hokkaido University Research > Institute Electronic Science, Japan > * Dr. Michelle Digman, Director Optical Biology Core Facility, > University of California, Irvine > * A/Professor Guy Cox, Australian Centre for Microscopy & > Microanalysis, University of Sydney > * Professor Leann Tilley, Department of Biochemistry, D/Director > Centre of Excellence in coherent X-ray Science, La Trobe University > * Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging > Facility, Garvan Institute of Medical Research, University of Sydney > * Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, > University of Sydney > * Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin > > Training sessions cover the following: > > Multi-colour Fluorescent proteins > Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby, > pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B > histone, actin, tubulin, Golgi, membrane); novel Photoactive > Fluorescent Proteins (EosFP, AmilRFP, kindling proteins, Phamret) from > reef corals. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein > localization & diffusion. > > Confocal Spectral Imaging > Acquisition of microspectral data (x, y, lambda) in 3D image stacks > from samples with multiple fluorescent probes or from fluorescent > coral tissues expressing a variety of GFP-type proteins (A. Salih > fluorescent corals > http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=he > alth)- Spectral unmixing, analysis, spectral FRET. > > Analysis of molecular movement and diffusion > Track proteins and other molecules in live cells. Measure protein > femtoliter concentrations. Monitor mobility and binding using > fluorescence correlation spectroscopy (FCS), scanning FCS, raster > image correlation spectroscopy (RICS), number & brightness (N&B) and > photon counting histogram (PCH). > > Fluorescence Lifetime Imaging Microscopy (FLIM) > - a powerful tool to analyse spatial distribution of excited state > lifetimes in samples: studied examples will include FRET-FLIM to study > protein interactions (e.g., DNA-Protein) in cells, imaging of > photoactivatable FPs, quenching of chlorophyll in plants, etc. > Hands-on training in FLIM and Phasor FLIM. > > > Workshop microscopes & companies > > Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto > optical beam splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B > Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging, > photon counting, FCS Nikon A1, Coherent Scientific Pty. Ltd - rapid > image acquisition, resonant scanner & 32 channel microspectral > detection Olymus FluoView FV1000 - variable barrier filter (VBF), > spectral detection, RICS and N&B > Ultra VIEW VoX spinning disc confocal microscope, high speed, > multichannel, 2D and 3D, FRAP, FLIP, photoactivation experiments, > PerkinElmer. > Confocal FLIM system, Becker & Hickl GmbH, Berlin A range of new > GFP-type protein constructs of many colours (cyan to far red) linked > to a variety of cellular proteins for in vivo protein localization > and dynamic studies, Evrogen And many more other instruments. > > > Registration > > Full workshop registration (lectures + training) will be limited to 35 > participants. > Registration will be on a first come first served basis. > > Contact Anya Salih a.salih@uws.edu.au to reserve your workshop place > Students $650 (GST inclusive) > All other $850 (GST inclusive) > > > Attendance of the BioImaging Expo on 16th November does not require > registration by please rsvp Pamela McMurtry > [pamela.mcmurtry@theconferenceteam.com.au] > Accommodation details at registration website, from $260 per week per > single room at UWS College. > Bus shuttle will be available between Sydney airport and the workshop > venue at UWS campus. > > Kind regards, Anya Dr Anya Salih Confocal Bio-Imaging Facility University Western Sydney Australia > 61 2 45701452 > a.salih@uws.edu.au ------------------------------ Message: 17 Date: Thu, 14 Oct 2010 12:07:46 +0100 From: "Edwards, Richard E." Subject: [Histonet] fridge/freezer storage space To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. ------------------------------ Message: 18 Date: Thu, 14 Oct 2010 06:49:54 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "'Amos Brooks'" , , Message-ID: <26E0799EA0D8454A86A3F86DF8A6F22D@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" Here here Amos. This is why I am for using multi tissue controls that have considered a range for most of the differences in tissues, fixation and processing encountered. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, October 13, 2010 4:21 PM To: JEllin@yumaregional.org; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.local > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 14 Oct 2010 08:58:25 -0400 From: John Shelley Subject: [Histonet] RE: fridge/freezer storage space To: "Edwards, Richard E." , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Richard, We are a research facility and have the same issue with not always knowing where and whose stuff is in the freezer or refrigerators. Even with the best system though you still have to have people who communicate that they are taking stuff out and also replacing back where it was removed from. We have instituted the use of a system called Freezer Pro. Here is the web address http://www.ruro.com/products/freezerpro.html At least this is a start, hope it helps. Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Thursday, October 14, 2010 7:08 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] fridge/freezer storage space Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 83, Issue 20 **************************************** From jo-ann.bader <@t> mcgill.ca Thu Oct 14 10:20:52 2010 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Thu Oct 14 10:25:15 2010 Subject: [Histonet] Histo-Processor Message-ID: <76D119EF12C904418800ED67CCB2062929974E62E1@EXMBXVS1.campus.mcgill.ca> We are looking for a new, smaller histo-processor. We would like a closed system, I really don't want one of those small round things that has to be kept under a hood. Anyone out there has a recommendation for a table top model. It is for student use so there would, probably never be more than 100 samples in the machine at one time. Thanks in advance Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From JWeems <@t> sjha.org Thu Oct 14 10:25:15 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Oct 14 10:25:20 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 References: <20101014130415337@smtp642.redcondor.net> <4CB6E512.6034.00E2.0@mont-hosp.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403987E61CD@CHEXCMS10.one.ads.che.org> The change is that you can bill per block now and not per specimen. This is for immunos and special stains. It does make a huge difference! Best, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Thursday, October 14, 2010 11:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. Thanks, Chris Evanish Montgomery Hospital Norristown PA Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mpence <@t> grhs.net Thu Oct 14 10:31:07 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Oct 14 10:31:14 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16403987E61CD@CHEXCMS10.one.ads.che.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974A57@is-e2k3.grhs.net> Can you site your source, please. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, October 14, 2010 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 The change is that you can bill per block now and not per specimen. This is for immunos and special stains. It does make a huge difference! Best, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Thursday, October 14, 2010 11:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. Thanks, Chris Evanish Montgomery Hospital Norristown PA Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michelecarr10 <@t> yahoo.com Thu Oct 14 10:40:30 2010 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Thu Oct 14 10:40:36 2010 Subject: [Histonet] Immunohistochemistry images Message-ID: <550632.33224.qm@web120719.mail.ne1.yahoo.com> Hi everyone, I was wondering if anyone knew of a website that I can view and save the IHC images.? We are putting together a new procedure manual and the pathogists want images of the antibodies we use to be included in the manual. Thanks in advance for your responses. Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca From RCazares <@t> schosp.org Thu Oct 14 10:45:45 2010 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Thu Oct 14 10:45:50 2010 Subject: [Histonet] Change in IHC billing Message-ID: <572D1F45B44B3D4096D554B4CB40639C032028CE01@EXCHCCRMB.schosp.org> Can anyone give a reference for this change in IHC billing. I'd like to have it in writing when I suggest the change for our lab. Thank you. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL ________________________________ *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From shive003 <@t> umn.edu Thu Oct 14 10:46:15 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Oct 14 10:46:21 2010 Subject: [Histonet] Immunohistochemistry images References: <550632.33224.qm@web120719.mail.ne1.yahoo.com> Message-ID: <37A6B945C1A24863A9589DD3580B095C@auxs.umn.edu> Try IHCWORLD Image Gallery. http://www.ihcworld.com/imagegallery/ Jan Shivers UMN VDL ----- Original Message ----- From: "Michele Carr" To: Sent: Thursday, October 14, 2010 10:40 AM Subject: [Histonet] Immunohistochemistry images Hi everyone, I was wondering if anyone knew of a website that I can view and save the IHC images. We are putting together a new procedure manual and the pathogists want images of the antibodies we use to be included in the manual. Thanks in advance for your responses. Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Thu Oct 14 10:26:55 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Oct 14 10:52:29 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <4CB6E512.6034.00E2.0@mont-hosp.com> References: <20101014130415337@smtp642.redcondor.net> <4CB6E512.6034.00E2.0@mont-hosp.com> Message-ID: <61135F0455D33347B5AAE209B903A30433DEBE6E@EXCHVS2.medctr.ad.wfubmc.edu> We have been told the same thing and just recently changed our billing practice. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Thursday, October 14, 2010 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. Thanks, Chris Evanish Montgomery Hospital Norristown PA Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. >>> 10/14/2010 9:04 AM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. pathology reports (Horn, Hazel V) 2. Seeking Grossing/Histo Tech (Mighnon Lashus) 3. Re: Urinary bladder diverticula (Robert Richmond) 4. pathology reports (Tench, Bill) 5. Re: Histonet Digest, Vol 83, Issue 19 (Cathy.Crumpton@tuality.org) 6. RE: RE: New Cap Guidelines for Her2 and ER/PR (Kuhnla, Melissa) 7. RE: RE: New Cap Guidelines for Her2 and ER/PR (Bill B.) 8. RE: RE: New Cap Guidelines for Her2 and ER/PR (McMahon, Loralee A) 9. FW: How to remove Hematoxilin (Margaryan, Naira) 10. I agree with Jose, etal. (Orr, Rebecca) 11. Training labs in the San Francisco Bay Area? (Morken, Tim) 12. RE: New Cap Guidelines for Her2 and ER/PR (Amos Brooks) 13. Re: Training labs in the San Francisco Bay Area? (Jennifer MacDonald) 14. Re: IHC OOps wrong secondary (Kimberly Tuttle) 15. "2010 Focus on IHC" one day event comes to NJ (Pedro Louro) 16. Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. (Anya Salih) 17. fridge/freezer storage space (Edwards, Richard E.) 18. RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR (Patsy Ruegg) 19. RE: fridge/freezer storage space (John Shelley) ---------------------------------------------------------------------- Message: 1 Date: Wed, 13 Oct 2010 12:06:50 -0500 From: "Horn, Hazel V" Subject: [Histonet] pathology reports To: "Histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB7181249D05B@EVS1.archildrens.org> Content-Type: text/plain; charset="ISO-8859-1" When you have results from an outside lab, i.e. flow results, bone marrows, ect. How do you integrate this into your path report? It is a verbatim copy inside the report in the same format as the outside lab? Or can it be a copy with the results in a different format? A discussion has occurred within in or transcription department over this matter. We do attach the outside report to the hard copy report in our paper file. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 2 Date: Wed, 13 Oct 2010 12:21:05 -0500 From: Mighnon Lashus Subject: [Histonet] Seeking Grossing/Histo Tech To: "histonet@lists.utsouthwestern.edu" Cc: Dewayne Belew Message-ID: <197CD0B02A81F94994A285C59C8AE05C05F49D4CDB@pgnexchange.pathgroup.com> Content-Type: text/plain; charset="us-ascii" We are seeking a qualified candidate for the position of Grossing/Lead Histo Tech in our Chattanooga location. We are a state of art laboratory using the Ventana Vantage system to maintain specimen integrity; we also have Ventana special stainers and the Benchmark Ultra and XTs for our Immunohistochemistry stains. We offer a friendly working environment along with an excellent benefit package. You may obtain more information about this position at CareerBuilders.com. We also have an opening for a cytotechnologist at our Pathology office on the Erlanger Health System campus in Chattanooga, TN. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ------------------------------ Message: 3 Date: Wed, 13 Oct 2010 13:26:33 -0400 From: Robert Richmond Subject: [Histonet] Re: Urinary bladder diverticula To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Joyce Weems in Atlanta asks: >>If TUR is 88307 and resection is 88309, what CPT code do you use for bladder diverticuli?<< A transurethral resection of the prostate (TURP) is coded 88305 no matter how many blocks. A TUR of the bladder (presumably a TURBT, that is, a TUR of a bladder tumor) is 88307. A cystectomy specimen for cancer (resection) is 88309. There are no specific instructions for a diverticulum of the urinary bladder. Diverticula of the GI tract are however coded 88305, and I would code a urinary tract diverticulum (bladder or urethra) as 88305 also. The singular is diverticulum, the plural is diverticula. There is no such word as *diverticuli. If you don't have a copy of the anatomic pathology CPT codes, 2010 edition, I can send you a PDF of a scan of those pages. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Wed, 13 Oct 2010 10:30:24 -0700 From: "Tench, Bill" Subject: [Histonet] pathology reports To: Histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56C2@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii We scan all outside laboratory reports (including consultations, flow cytometry, special immunohistochemistry, molecular tests, etc) and insert the scanned material into an addendum which is electronically tied to the primary report. If the outside report has graphic material (ie, material that cannot be converted into a "WORD" format) it has to be excised because at least our vision of Cerner Millenium will not permit non-text material to be inserted into the report document (We use an Epson scanner and Epson program that does a very handy job of converting PDF documents into WORD documents and allows for the excision of non-WORD material---like fancy headers). When appropriate, ie, it is not obvious what the results mean, we may add our own comment about how these results should be interpreted in the setting of our other material. This saves a lot of transcription (which use to be the way we accomplished this) and avoids transcriptional errors. It generally allows capture of the name and address of the outside laboratory, which is a CLIA requirement. Incorporating the results of special tests into the report is a CAP requirement. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 5 Date: Wed, 13 Oct 2010 10:40:39 -0700 From: Cathy.Crumpton@tuality.org Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 19 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" We are not open on weekends and worked out a deal with our core l that is open 24/7. When we have a breast on the processer we will run a Sat. program that ends at 21:00. They added a task on their dai They j were being embed the embedding cent embedding. No harm Cathy Tuality Community Hospital Hillsbo (503)681-1292 ------------------------------ Message: 6 Date: Wed, 13 Oct 2010 13:51:45 -0400 From: "Kuhnla, Melissa" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Mike Pence , Phyllis Thaxton , "Mahoney,Janice A" , Joyce Cline , Message-ID: Content-Type: text/plain; charset="US-ASCII" Fixation prior to the processor will not exce4ed 12 hrs. That is why we program the processor for 36...not to exceed 48. ________________________________ From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, October 13, 2010 11:04 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than "normal processing" time in 70% (ie. more than 1 hr). -----Original Message----- From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. 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Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ------------------------------ Message: 7 Date: Wed, 13 Oct 2010 14:12:59 -0500 From: "Bill B." Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Message-ID: Content-Type: text/plain; charset="us-ascii" How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org ------------------------------ Message: 8 Date: Wed, 13 Oct 2010 15:23:10 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Bill B." , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" If our cases are needle cores they are almost immediately put into formalin from the patient. That time is recorded by the nurse or the clinician that took the specimen. The larger breast samples are received fresh from the OR and are immediately grossed either by a PA or resident. Those samples are examined, sliced (to expose the surface area of the specimen) and placed into formalin. That is when the fixation time is recorded for the larger specimens. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. [bill501@mindspring.com] Sent: Wednesday, October 13, 2010 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 13 Oct 2010 16:03:28 -0500 From: "Margaryan, Naira" Subject: [Histonet] FW: How to remove Hematoxilin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histoworld, I would like to repeat my staining on the slides already coverslipped but need to remove hematoxilin first. How to remove hematoxilin? Is it need to repeat Antigen retrieval? Thanks in advance, Naira ------------------------------ Message: 10 Date: Wed, 13 Oct 2010 16:29:49 -0500 From: "Orr, Rebecca" Subject: [Histonet] I agree with Jose, etal. To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Thanks for your response, Jose, I agree with your points. I see these guidelines as the first attempt to standardize something that has numerous variables. How can we expect to have the manufacturers of these markers offer (or be required by the FDA) consistent results when our end of this process is "all over the board"? An ER, PR Her2 that we both buy from the same manufacturer should work the exact same way in my lab as it does in yours, IF we both process in the exact same way. I doubt very much if histology and IHC testing will ever be as exact as a clinical chemistry assay for example, but it's a start. I forsee the time gap in formalin shortening as labs get used to this first step. This would mean more validation, but since we should be doing validation each year, then this may not be such a giant task. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ------------------------------ Message: 11 Date: Wed, 13 Oct 2010 15:12:54 -0700 From: "Morken, Tim" Subject: [Histonet] Training labs in the San Francisco Bay Area? To: Histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C026967E880@EXMBMCB15.ucsfmedicalcenter. org> Content-Type: text/plain; charset=us-ascii To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org ------------------------------ Message: 12 Date: Wed, 13 Oct 2010 18:21:22 -0400 From: Amos Brooks Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: JEllin@yumaregional.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.l ocal > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin ------------------------------ Message: 13 Date: Wed, 13 Oct 2010 15:30:21 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Training labs in the San Francisco Bay Area? To: "Morken, Tim" Cc: Histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" With the on-line programs the training facility will need to be approved by the program. They will need to sign an affiliation agreement between the program (school) and the training site. "Morken, Tim" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/13/2010 03:17 PM To Histonet cc Subject [Histonet] Training labs in the San Francisco Bay Area? To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 13 Oct 2010 19:14:57 -0400 From: "Kimberly Tuttle" Subject: Re: [Histonet] IHC OOps wrong secondary To: "histonet@lists.utsouthwestern.edu" , "Kimberly Tuttle" Message-ID: <4CB60536.90CE.001A.3@umm.edu> Content-Type: text/plain; charset=US-ASCII Thanks to everyone who responded. I removed the coverslip, and ran it back to water and re applied the secondary, dab and counterstain. It worked like a charm. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >>> "Kimberly Tuttle" 10/13/2010 12:07 pm >>> Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 15 Date: Wed, 13 Oct 2010 20:34:17 -0400 From: Pedro Louro Subject: [Histonet] "2010 Focus on IHC" one day event comes to NJ To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The date is set, the topics are in place, and the money is just right, all we need....is you to attend. When: Friday, November 5, 2010 Where: Somerset Medical Center 110 Rehill Ave Somerville, NJ 08876 *"2010 Focus on IHC"* presented by NJSH and sponsored by BioCare Medical, LLC $10.00(members) $40.00 (Non-members) gets you: - 6 CEU's - Breakfast, Lunch and Snacks - Great learning experience Hope to see you there, Pedro Louro President (New Jersey Society for Histotechnology) Co-chair Membership Committee ________________________________________________________________________ ___ *Meeting Schedule * 7:30-8:30AM Registration and Continental Breakfast * 8:30-12:00 *AM Session with Coffee Break * Seminars * Mouse Models and IHC; Linda Dean Antibodies 2010; Fatima Natar * OR ** Wet Workshop * Multiplex Staining in the Anatomic Laboratory; Tara Kennedy * 12:00-1:00 *Lunch (wraps, salad, soup, dessert) * 1:00-4:30 *PM Session with Coffee Break * Seminars * CAP Regulations; Terry Murphy Validation in the IHC Laboratory; George Hoernig * OR ** Wet Workshop * Rapid In Situ Hybridization; Will Chappell ------------------------------ Message: 16 Date: Thu, 14 Oct 2010 15:34:27 +1100 From: "Anya Salih" Subject: [Histonet] Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. To: Message-ID: <512D5A4F81BF054F9735F9C0AC45AB3B0281BC51@VIOLA.AD.UWS.EDU.AU> Content-Type: text/plain; charset="us-ascii" > You and your students are invited to attend the 3rd Advanced > Bio-Imaging Workshop at the University of Western Sydney on 16 - 19 > November, 2010 and the Bio-Imaging Expo (free event on 16th November > 2010) > > Tracking Molecules with Light > > Training in confocal imaging and protein 3D tracking, aggregation, > diffusion analyses. Experiments will involve mammalian cell lines, > invertebrate (coral) cells, plant and algae, bacteria and other > samples. > > Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop. > > Places limited to 35 and only 20 places left so register now. > > > Location: Confocal Bio-Imaging Facility, Building S8, Hawkesbury > Campus, University of Western Sydney > Organiser: Dr Anya Salih > Training by: Dr Salih UWS; Prof. E. Gratton and Dr M. Digman Univ. > California; Prof. Guy Cox Uni Sydney; Dr Wolfgang Becker, Becker & > Hickl GmbH Germany; Dr C Thoni Leica Microsystems, G. Symonds Zeiss) > > > Lectures and intensive hands-on training on confocal microscopes by > top researchers in the field. Learn how to explore and analyse the 3D > structural complexity of invertebrate, animal & plant cells, tissues > and micro-organisms, visualize and analyse movement of organelles and > molecules. Trial a range of novel GFP-type protein constructs. Discuss > your experiments and trial new approaches. Workshop emphasis on > advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B, > FLIM, photoactivatable fluorescent proteins. > > Invited speakers > > * Professor Enrico Gratton, Director Laboratory for Fluorescence > Dynamics, University of California, Irvine > * Professor Takeharu Nagai, Laboratory for Nanosystems > Physiology & Nikon Imaging Center, Hokkaido University Research > Institute Electronic Science, Japan > * Dr. Michelle Digman, Director Optical Biology Core Facility, > University of California, Irvine > * A/Professor Guy Cox, Australian Centre for Microscopy & > Microanalysis, University of Sydney > * Professor Leann Tilley, Department of Biochemistry, D/Director > Centre of Excellence in coherent X-ray Science, La Trobe University > * Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging > Facility, Garvan Institute of Medical Research, University of Sydney > * Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, > University of Sydney > * Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin > > Training sessions cover the following: > > Multi-colour Fluorescent proteins > Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby, > pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B > histone, actin, tubulin, Golgi, membrane); novel Photoactive > Fluorescent Proteins (EosFP, AmilRFP, kindling proteins, Phamret) from > reef corals. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein > localization & diffusion. > > Confocal Spectral Imaging > Acquisition of microspectral data (x, y, lambda) in 3D image stacks > from samples with multiple fluorescent probes or from fluorescent > coral tissues expressing a variety of GFP-type proteins (A. Salih > fluorescent corals > http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=he > alth)- Spectral unmixing, analysis, spectral FRET. > > Analysis of molecular movement and diffusion > Track proteins and other molecules in live cells. Measure protein > femtoliter concentrations. Monitor mobility and binding using > fluorescence correlation spectroscopy (FCS), scanning FCS, raster > image correlation spectroscopy (RICS), number & brightness (N&B) and > photon counting histogram (PCH). > > Fluorescence Lifetime Imaging Microscopy (FLIM) > - a powerful tool to analyse spatial distribution of excited state > lifetimes in samples: studied examples will include FRET-FLIM to study > protein interactions (e.g., DNA-Protein) in cells, imaging of > photoactivatable FPs, quenching of chlorophyll in plants, etc. > Hands-on training in FLIM and Phasor FLIM. > > > Workshop microscopes & companies > > Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto > optical beam splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B > Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging, > photon counting, FCS Nikon A1, Coherent Scientific Pty. Ltd - rapid > image acquisition, resonant scanner & 32 channel microspectral > detection Olymus FluoView FV1000 - variable barrier filter (VBF), > spectral detection, RICS and N&B > Ultra VIEW VoX spinning disc confocal microscope, high speed, > multichannel, 2D and 3D, FRAP, FLIP, photoactivation experiments, > PerkinElmer. > Confocal FLIM system, Becker & Hickl GmbH, Berlin A range of new > GFP-type protein constructs of many colours (cyan to far red) linked > to a variety of cellular proteins for in vivo protein localization > and dynamic studies, Evrogen And many more other instruments. > > > Registration > > Full workshop registration (lectures + training) will be limited to 35 > participants. > Registration will be on a first come first served basis. > > Contact Anya Salih a.salih@uws.edu.au to reserve your workshop place > Students $650 (GST inclusive) > All other $850 (GST inclusive) > > > Attendance of the BioImaging Expo on 16th November does not require > registration by please rsvp Pamela McMurtry > [pamela.mcmurtry@theconferenceteam.com.au] > Accommodation details at registration website, from $260 per week per > single room at UWS College. > Bus shuttle will be available between Sydney airport and the workshop > venue at UWS campus. > > Kind regards, Anya Dr Anya Salih Confocal Bio-Imaging Facility University Western Sydney Australia > 61 2 45701452 > a.salih@uws.edu.au ------------------------------ Message: 17 Date: Thu, 14 Oct 2010 12:07:46 +0100 From: "Edwards, Richard E." Subject: [Histonet] fridge/freezer storage space To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. ------------------------------ Message: 18 Date: Thu, 14 Oct 2010 06:49:54 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "'Amos Brooks'" , , Message-ID: <26E0799EA0D8454A86A3F86DF8A6F22D@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" Here here Amos. This is why I am for using multi tissue controls that have considered a range for most of the differences in tissues, fixation and processing encountered. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, October 13, 2010 4:21 PM To: JEllin@yumaregional.org; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.l ocal > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 14 Oct 2010 08:58:25 -0400 From: John Shelley Subject: [Histonet] RE: fridge/freezer storage space To: "Edwards, Richard E." , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Richard, We are a research facility and have the same issue with not always knowing where and whose stuff is in the freezer or refrigerators. Even with the best system though you still have to have people who communicate that they are taking stuff out and also replacing back where it was removed from. We have instituted the use of a system called Freezer Pro. Here is the web address http://www.ruro.com/products/freezerpro.html At least this is a start, hope it helps. Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Thursday, October 14, 2010 7:08 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] fridge/freezer storage space Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 83, Issue 20 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Oct 14 10:55:28 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Oct 14 10:55:40 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <61135F0455D33347B5AAE209B903A30433DEBE6E@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974A58@is-e2k3.grhs.net> Did you verify this before you made such a huge change. I would like to see a source before I make any changes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Thursday, October 14, 2010 10:27 AM To: Chris Evanish; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 We have been told the same thing and just recently changed our billing practice. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Thursday, October 14, 2010 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. Thanks, Chris Evanish Montgomery Hospital Norristown PA Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. >>> 10/14/2010 9:04 AM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. pathology reports (Horn, Hazel V) 2. Seeking Grossing/Histo Tech (Mighnon Lashus) 3. Re: Urinary bladder diverticula (Robert Richmond) 4. pathology reports (Tench, Bill) 5. Re: Histonet Digest, Vol 83, Issue 19 (Cathy.Crumpton@tuality.org) 6. RE: RE: New Cap Guidelines for Her2 and ER/PR (Kuhnla, Melissa) 7. RE: RE: New Cap Guidelines for Her2 and ER/PR (Bill B.) 8. RE: RE: New Cap Guidelines for Her2 and ER/PR (McMahon, Loralee A) 9. FW: How to remove Hematoxilin (Margaryan, Naira) 10. I agree with Jose, etal. (Orr, Rebecca) 11. Training labs in the San Francisco Bay Area? (Morken, Tim) 12. RE: New Cap Guidelines for Her2 and ER/PR (Amos Brooks) 13. Re: Training labs in the San Francisco Bay Area? (Jennifer MacDonald) 14. Re: IHC OOps wrong secondary (Kimberly Tuttle) 15. "2010 Focus on IHC" one day event comes to NJ (Pedro Louro) 16. Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. (Anya Salih) 17. fridge/freezer storage space (Edwards, Richard E.) 18. RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR (Patsy Ruegg) 19. RE: fridge/freezer storage space (John Shelley) ---------------------------------------------------------------------- Message: 1 Date: Wed, 13 Oct 2010 12:06:50 -0500 From: "Horn, Hazel V" Subject: [Histonet] pathology reports To: "Histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB7181249D05B@EVS1.archildrens.org> Content-Type: text/plain; charset="ISO-8859-1" When you have results from an outside lab, i.e. flow results, bone marrows, ect. How do you integrate this into your path report? It is a verbatim copy inside the report in the same format as the outside lab? Or can it be a copy with the results in a different format? A discussion has occurred within in or transcription department over this matter. We do attach the outside report to the hard copy report in our paper file. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 2 Date: Wed, 13 Oct 2010 12:21:05 -0500 From: Mighnon Lashus Subject: [Histonet] Seeking Grossing/Histo Tech To: "histonet@lists.utsouthwestern.edu" Cc: Dewayne Belew Message-ID: <197CD0B02A81F94994A285C59C8AE05C05F49D4CDB@pgnexchange.pathgroup.com> Content-Type: text/plain; charset="us-ascii" We are seeking a qualified candidate for the position of Grossing/Lead Histo Tech in our Chattanooga location. We are a state of art laboratory using the Ventana Vantage system to maintain specimen integrity; we also have Ventana special stainers and the Benchmark Ultra and XTs for our Immunohistochemistry stains. We offer a friendly working environment along with an excellent benefit package. You may obtain more information about this position at CareerBuilders.com. We also have an opening for a cytotechnologist at our Pathology office on the Erlanger Health System campus in Chattanooga, TN. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ------------------------------ Message: 3 Date: Wed, 13 Oct 2010 13:26:33 -0400 From: Robert Richmond Subject: [Histonet] Re: Urinary bladder diverticula To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Joyce Weems in Atlanta asks: >>If TUR is 88307 and resection is 88309, what CPT code do you use for bladder diverticuli?<< A transurethral resection of the prostate (TURP) is coded 88305 no matter how many blocks. A TUR of the bladder (presumably a TURBT, that is, a TUR of a bladder tumor) is 88307. A cystectomy specimen for cancer (resection) is 88309. There are no specific instructions for a diverticulum of the urinary bladder. Diverticula of the GI tract are however coded 88305, and I would code a urinary tract diverticulum (bladder or urethra) as 88305 also. The singular is diverticulum, the plural is diverticula. There is no such word as *diverticuli. If you don't have a copy of the anatomic pathology CPT codes, 2010 edition, I can send you a PDF of a scan of those pages. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Wed, 13 Oct 2010 10:30:24 -0700 From: "Tench, Bill" Subject: [Histonet] pathology reports To: Histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56C2@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii We scan all outside laboratory reports (including consultations, flow cytometry, special immunohistochemistry, molecular tests, etc) and insert the scanned material into an addendum which is electronically tied to the primary report. If the outside report has graphic material (ie, material that cannot be converted into a "WORD" format) it has to be excised because at least our vision of Cerner Millenium will not permit non-text material to be inserted into the report document (We use an Epson scanner and Epson program that does a very handy job of converting PDF documents into WORD documents and allows for the excision of non-WORD material---like fancy headers). When appropriate, ie, it is not obvious what the results mean, we may add our own comment about how these results should be interpreted in the setting of our other material. This saves a lot of transcription (which use to be the way we accomplished this) and avoids transcriptional errors. It generally allows capture of the name and address of the outside laboratory, which is a CLIA requirement. Incorporating the results of special tests into the report is a CAP requirement. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 5 Date: Wed, 13 Oct 2010 10:40:39 -0700 From: Cathy.Crumpton@tuality.org Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 19 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" We are not open on weekends and worked out a deal with our core l that is open 24/7. When we have a breast on the processer we will run a Sat. program that ends at 21:00. They added a task on their dai They j were being embed the embedding cent embedding. No harm Cathy Tuality Community Hospital Hillsbo (503)681-1292 ------------------------------ Message: 6 Date: Wed, 13 Oct 2010 13:51:45 -0400 From: "Kuhnla, Melissa" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Mike Pence , Phyllis Thaxton , "Mahoney,Janice A" , Joyce Cline , Message-ID: Content-Type: text/plain; charset="US-ASCII" Fixation prior to the processor will not exce4ed 12 hrs. That is why we program the processor for 36...not to exceed 48. ________________________________ From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, October 13, 2010 11:04 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than "normal processing" time in 70% (ie. more than 1 hr). -----Original Message----- From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ------------------------------ Message: 7 Date: Wed, 13 Oct 2010 14:12:59 -0500 From: "Bill B." Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Message-ID: Content-Type: text/plain; charset="us-ascii" How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > For the weekend, we have our processor set for 36 hours in formalin >and then a hold in 70%. This allows for complete fixation and cuts down >on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the tissues >are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order >to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > Formalin fixation time does not impact the results of FISH as it does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time is >still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org ------------------------------ Message: 8 Date: Wed, 13 Oct 2010 15:23:10 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Bill B." , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" If our cases are needle cores they are almost immediately put into formalin from the patient. That time is recorded by the nurse or the clinician that took the specimen. The larger breast samples are received fresh from the OR and are immediately grossed either by a PA or resident. Those samples are examined, sliced (to expose the surface area of the specimen) and placed into formalin. That is when the fixation time is recorded for the larger specimens. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. [bill501@mindspring.com] Sent: Wednesday, October 13, 2010 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 13 Oct 2010 16:03:28 -0500 From: "Margaryan, Naira" Subject: [Histonet] FW: How to remove Hematoxilin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histoworld, I would like to repeat my staining on the slides already coverslipped but need to remove hematoxilin first. How to remove hematoxilin? Is it need to repeat Antigen retrieval? Thanks in advance, Naira ------------------------------ Message: 10 Date: Wed, 13 Oct 2010 16:29:49 -0500 From: "Orr, Rebecca" Subject: [Histonet] I agree with Jose, etal. To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Thanks for your response, Jose, I agree with your points. I see these guidelines as the first attempt to standardize something that has numerous variables. How can we expect to have the manufacturers of these markers offer (or be required by the FDA) consistent results when our end of this process is "all over the board"? An ER, PR Her2 that we both buy from the same manufacturer should work the exact same way in my lab as it does in yours, IF we both process in the exact same way. I doubt very much if histology and IHC testing will ever be as exact as a clinical chemistry assay for example, but it's a start. I forsee the time gap in formalin shortening as labs get used to this first step. This would mean more validation, but since we should be doing validation each year, then this may not be such a giant task. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ------------------------------ Message: 11 Date: Wed, 13 Oct 2010 15:12:54 -0700 From: "Morken, Tim" Subject: [Histonet] Training labs in the San Francisco Bay Area? To: Histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C026967E880@EXMBMCB15.ucsfmedicalcenter. org> Content-Type: text/plain; charset=us-ascii To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org ------------------------------ Message: 12 Date: Wed, 13 Oct 2010 18:21:22 -0400 From: Amos Brooks Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: JEllin@yumaregional.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.l ocal > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin ------------------------------ Message: 13 Date: Wed, 13 Oct 2010 15:30:21 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Training labs in the San Francisco Bay Area? To: "Morken, Tim" Cc: Histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" With the on-line programs the training facility will need to be approved by the program. They will need to sign an affiliation agreement between the program (school) and the training site. "Morken, Tim" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/13/2010 03:17 PM To Histonet cc Subject [Histonet] Training labs in the San Francisco Bay Area? To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 13 Oct 2010 19:14:57 -0400 From: "Kimberly Tuttle" Subject: Re: [Histonet] IHC OOps wrong secondary To: "histonet@lists.utsouthwestern.edu" , "Kimberly Tuttle" Message-ID: <4CB60536.90CE.001A.3@umm.edu> Content-Type: text/plain; charset=US-ASCII Thanks to everyone who responded. I removed the coverslip, and ran it back to water and re applied the secondary, dab and counterstain. It worked like a charm. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >>> "Kimberly Tuttle" 10/13/2010 12:07 pm >>> Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 15 Date: Wed, 13 Oct 2010 20:34:17 -0400 From: Pedro Louro Subject: [Histonet] "2010 Focus on IHC" one day event comes to NJ To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The date is set, the topics are in place, and the money is just right, all we need....is you to attend. When: Friday, November 5, 2010 Where: Somerset Medical Center 110 Rehill Ave Somerville, NJ 08876 *"2010 Focus on IHC"* presented by NJSH and sponsored by BioCare Medical, LLC $10.00(members) $40.00 (Non-members) gets you: - 6 CEU's - Breakfast, Lunch and Snacks - Great learning experience Hope to see you there, Pedro Louro President (New Jersey Society for Histotechnology) Co-chair Membership Committee ________________________________________________________________________ ___ *Meeting Schedule * 7:30-8:30AM Registration and Continental Breakfast * 8:30-12:00 *AM Session with Coffee Break * Seminars * Mouse Models and IHC; Linda Dean Antibodies 2010; Fatima Natar * OR ** Wet Workshop * Multiplex Staining in the Anatomic Laboratory; Tara Kennedy * 12:00-1:00 *Lunch (wraps, salad, soup, dessert) * 1:00-4:30 *PM Session with Coffee Break * Seminars * CAP Regulations; Terry Murphy Validation in the IHC Laboratory; George Hoernig * OR ** Wet Workshop * Rapid In Situ Hybridization; Will Chappell ------------------------------ Message: 16 Date: Thu, 14 Oct 2010 15:34:27 +1100 From: "Anya Salih" Subject: [Histonet] Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. To: Message-ID: <512D5A4F81BF054F9735F9C0AC45AB3B0281BC51@VIOLA.AD.UWS.EDU.AU> Content-Type: text/plain; charset="us-ascii" > You and your students are invited to attend the 3rd Advanced > Bio-Imaging Workshop at the University of Western Sydney on 16 - 19 > November, 2010 and the Bio-Imaging Expo (free event on 16th November > 2010) > > Tracking Molecules with Light > > Training in confocal imaging and protein 3D tracking, aggregation, > diffusion analyses. Experiments will involve mammalian cell lines, > invertebrate (coral) cells, plant and algae, bacteria and other > samples. > > Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop. > > Places limited to 35 and only 20 places left so register now. > > > Location: Confocal Bio-Imaging Facility, Building S8, Hawkesbury > Campus, University of Western Sydney > Organiser: Dr Anya Salih > Training by: Dr Salih UWS; Prof. E. Gratton and Dr M. Digman Univ. > California; Prof. Guy Cox Uni Sydney; Dr Wolfgang Becker, Becker & > Hickl GmbH Germany; Dr C Thoni Leica Microsystems, G. Symonds Zeiss) > > > Lectures and intensive hands-on training on confocal microscopes by > top researchers in the field. Learn how to explore and analyse the 3D > structural complexity of invertebrate, animal & plant cells, tissues > and micro-organisms, visualize and analyse movement of organelles and > molecules. Trial a range of novel GFP-type protein constructs. Discuss > your experiments and trial new approaches. Workshop emphasis on > advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B, > FLIM, photoactivatable fluorescent proteins. > > Invited speakers > > * Professor Enrico Gratton, Director Laboratory for Fluorescence > Dynamics, University of California, Irvine > * Professor Takeharu Nagai, Laboratory for Nanosystems > Physiology & Nikon Imaging Center, Hokkaido University Research > Institute Electronic Science, Japan > * Dr. Michelle Digman, Director Optical Biology Core Facility, > University of California, Irvine > * A/Professor Guy Cox, Australian Centre for Microscopy & > Microanalysis, University of Sydney > * Professor Leann Tilley, Department of Biochemistry, D/Director > Centre of Excellence in coherent X-ray Science, La Trobe University > * Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging > Facility, Garvan Institute of Medical Research, University of Sydney > * Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, > University of Sydney > * Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin > > Training sessions cover the following: > > Multi-colour Fluorescent proteins > Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby, > pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B > histone, actin, tubulin, Golgi, membrane); novel Photoactive > Fluorescent Proteins (EosFP, AmilRFP, kindling proteins, Phamret) from > reef corals. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein > localization & diffusion. > > Confocal Spectral Imaging > Acquisition of microspectral data (x, y, lambda) in 3D image stacks > from samples with multiple fluorescent probes or from fluorescent > coral tissues expressing a variety of GFP-type proteins (A. Salih > fluorescent corals > http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=he > alth)- Spectral unmixing, analysis, spectral FRET. > > Analysis of molecular movement and diffusion > Track proteins and other molecules in live cells. Measure protein > femtoliter concentrations. Monitor mobility and binding using > fluorescence correlation spectroscopy (FCS), scanning FCS, raster > image correlation spectroscopy (RICS), number & brightness (N&B) and > photon counting histogram (PCH). > > Fluorescence Lifetime Imaging Microscopy (FLIM) > - a powerful tool to analyse spatial distribution of excited state > lifetimes in samples: studied examples will include FRET-FLIM to study > protein interactions (e.g., DNA-Protein) in cells, imaging of > photoactivatable FPs, quenching of chlorophyll in plants, etc. > Hands-on training in FLIM and Phasor FLIM. > > > Workshop microscopes & companies > > Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto > optical beam splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B > Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging, > photon counting, FCS Nikon A1, Coherent Scientific Pty. Ltd - rapid > image acquisition, resonant scanner & 32 channel microspectral > detection Olymus FluoView FV1000 - variable barrier filter (VBF), > spectral detection, RICS and N&B > Ultra VIEW VoX spinning disc confocal microscope, high speed, > multichannel, 2D and 3D, FRAP, FLIP, photoactivation experiments, > PerkinElmer. > Confocal FLIM system, Becker & Hickl GmbH, Berlin A range of new > GFP-type protein constructs of many colours (cyan to far red) linked > to a variety of cellular proteins for in vivo protein localization > and dynamic studies, Evrogen And many more other instruments. > > > Registration > > Full workshop registration (lectures + training) will be limited to 35 > participants. Registration will be on a first come first served basis. > > Contact Anya Salih a.salih@uws.edu.au to reserve your workshop place > Students $650 (GST inclusive) > All other $850 (GST inclusive) > > > Attendance of the BioImaging Expo on 16th November does not require > registration by please rsvp Pamela McMurtry > [pamela.mcmurtry@theconferenceteam.com.au] > Accommodation details at registration website, from $260 per week per > single room at UWS College. Bus shuttle will be available between > Sydney airport and the workshop venue at UWS campus. > > Kind regards, Anya Dr Anya Salih Confocal Bio-Imaging Facility University Western Sydney Australia > 61 2 45701452 > a.salih@uws.edu.au ------------------------------ Message: 17 Date: Thu, 14 Oct 2010 12:07:46 +0100 From: "Edwards, Richard E." Subject: [Histonet] fridge/freezer storage space To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. ------------------------------ Message: 18 Date: Thu, 14 Oct 2010 06:49:54 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "'Amos Brooks'" , , Message-ID: <26E0799EA0D8454A86A3F86DF8A6F22D@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" Here here Amos. This is why I am for using multi tissue controls that have considered a range for most of the differences in tissues, fixation and processing encountered. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, October 13, 2010 4:21 PM To: JEllin@yumaregional.org; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.l ocal > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 14 Oct 2010 08:58:25 -0400 From: John Shelley Subject: [Histonet] RE: fridge/freezer storage space To: "Edwards, Richard E." , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Richard, We are a research facility and have the same issue with not always knowing where and whose stuff is in the freezer or refrigerators. Even with the best system though you still have to have people who communicate that they are taking stuff out and also replacing back where it was removed from. We have instituted the use of a system called Freezer Pro. Here is the web address http://www.ruro.com/products/freezerpro.html At least this is a start, hope it helps. Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Thursday, October 14, 2010 7:08 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] fridge/freezer storage space Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 83, Issue 20 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Thu Oct 14 11:02:38 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Thu Oct 14 11:02:47 2010 Subject: [Histonet] change in CPT coding rule Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56CD@MAIL1.pph.local> The change in place now allows for billing multiple blocks from the same specimen for the same special stain (it isn't just immunos) BUT you must justify this (should be done by a statement in the report micro). You are not permitted just because you wanted to stain multiple blocks to increase your chance of identifying the target, rather you must indicate that EACH additional block presents additional information. This could be assumed to always be the case for sentinel nodes (you are specifically looking for tumor in each block), but would not be true if you have a multiple blocks of a lung tumor that you were testing for some specifc marker. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From STACEY.LANGENBERG <@t> UCDENVER.EDU Thu Oct 14 11:03:01 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Thu Oct 14 11:03:11 2010 Subject: [Histonet] Histo-Processor In-Reply-To: <76D119EF12C904418800ED67CCB2062929974E62E1@EXMBXVS1.campus.mcgill.ca> References: <76D119EF12C904418800ED67CCB2062929974E62E1@EXMBXVS1.campus.mcgill.ca> Message-ID: <1230312577.2252712.1287072184786.JavaMail.rim@bda2340.bisx.prod.on.blackberry> Hi Jo-Ann We are trying to sell a VIP 3000 right now. It is closed and has really cool magnets to operate it. It is in good shape and a real work horse. It holds 300 blocks and is a benchtop model. Please let me know if you are interested. Thanks Stacey Langenberg CU Dermpath Sent via BlackBerry from T-Mobile -----Original Message----- From: "Jo-Ann Bader, Ms." Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Thu, 14 Oct 2010 09:20:52 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histo-Processor We are looking for a new, smaller histo-processor. We would like a closed system, I really don't want one of those small round things that has to be kept under a hood. Anyone out there has a recommendation for a table top model. It is for student use so there would, probably never be more than 100 samples in the machine at one time. Thanks in advance Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Oct 14 11:04:55 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 14 11:05:00 2010 Subject: [Histonet] Immunohistochemistry images In-Reply-To: <550632.33224.qm@web120719.mail.ne1.yahoo.com> References: <550632.33224.qm@web120719.mail.ne1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EB2133FEA@IBMB7Exchange.digestivespecialists.com> Try the web site of the vender that you get your antibodies from. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Carr Sent: Thursday, October 14, 2010 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunohistochemistry images Hi everyone, I was wondering if anyone knew of a website that I can view and save the IHC images.? We are putting together a new procedure manual and the pathogists want images of the antibodies we use to be included in the manual. Thanks in advance for your responses. Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Oct 14 11:22:41 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Oct 14 11:22:48 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974A57@is-e2k3.grhs.net> References: <92AD9B20A6C38C4587A9FEBE3A30E16403987E61CD@CHEXCMS10.one.ads.che.org> <661949901A768E4F9CC16D8AF8F2838C03974A57@is-e2k3.grhs.net> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403987E6200@CHEXCMS10.one.ads.che.org> CMS/NCCI Update Dated October 1, 2009 8. The unit of service for special stains (CPT codes 88312-88313) and immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If it is medically reasonable and necessary to perform the same stain on more than one specimen or more than one block of tissue from the same specimen, additional units of service may be reported for the additional specimen(s) or block(s). Physicians should not report more than one unit of service for a stain performed on a single tissue block. For example it is common practice to cut multiple levels from a tissue block and stain each level with the same stain. The multiple levels from the same block of tissue stained with the same stain should not be reported as additional units of service. Only one unit of service should be reported for the stain on multiple levels from the single tissue block. Additionally, controls performed with special stains should not be reported as separate units of service for the stain. -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Thursday, October 14, 2010 11:31 To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you site your source, please. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, October 14, 2010 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 The change is that you can bill per block now and not per specimen. This is for immunos and special stains. It does make a huge difference! Best, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Thursday, October 14, 2010 11:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. Thanks, Chris Evanish Montgomery Hospital Norristown PA Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From alyssa <@t> alliedsearchpartners.com Thu Oct 14 11:29:51 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Oct 14 11:29:56 2010 Subject: [Histonet] Dermpath Job Openings Message-ID: Hello, I am just sending this email out because I am working on a couple Dermatopathologist job openings. Please let me know if anyone out there is interested or knows someone interested (we offer referral bonuses) in a Dermatopathologist poistion in Atlanta, GA area or in the Milwaukee, WI area. One of the main requirements is that we are looking for a Dermatopathologist with at least Dermatology residency, they do not necessarily have to have experience working as a Dermatologist, however, we are looking for at least the residency. Thank you in advance for sending anyone my way, and I will give them more details! -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From RCazares <@t> schosp.org Thu Oct 14 11:29:58 2010 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Thu Oct 14 11:30:04 2010 Subject: [SPAM-HC] - RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 - Email found in subject In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16403987E6200@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E16403987E61CD@CHEXCMS10.one.ads.che.org><661949901A768E4F9CC16D8AF8F2838C03974A57@is-e2k3.grhs.net> <92AD9B20A6C38C4587A9FEBE3A30E16403987E6200@CHEXCMS10.one.ads.che.org> Message-ID: <572D1F45B44B3D4096D554B4CB40639C032028CE77@EXCHCCRMB.schosp.org> Thank you so much for the source information! Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, October 14, 2010 11:23 AM To: Mike Pence; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 - Email found in subject CMS/NCCI Update Dated October 1, 2009 8. The unit of service for special stains (CPT codes 88312-88313) and immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If it is medically reasonable and necessary to perform the same stain on more than one specimen or more than one block of tissue from the same specimen, additional units of service may be reported for the additional specimen(s) or block(s). Physicians should not report more than one unit of service for a stain performed on a single tissue block. For example it is common practice to cut multiple levels from a tissue block and stain each level with the same stain. The multiple levels from the same block of tissue stained with the same stain should not be reported as additional units of service. Only one unit of service should be reported for the stain on multiple levels from the single tissue block. Additionally, controls performed with special stains should not be reported as separate units of service for the stain. -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Thursday, October 14, 2010 11:31 To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you site your source, please. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, October 14, 2010 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 The change is that you can bill per block now and not per specimen. This is for immunos and special stains. It does make a huge difference! Best, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Thursday, October 14, 2010 11:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. Thanks, Chris Evanish Montgomery Hospital Norristown PA Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From victoria.spoon <@t> bassett.org Thu Oct 14 11:53:21 2010 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Thu Oct 14 11:53:36 2010 Subject: [Histonet] Locking up formalin Message-ID: Is anyone aware of regulations stating that formalin has to be locked up- put in locked cabinets when not under direct supervision? Applying to either clinics where specimens are collected into formalin containers or in the pathology lab? Thank you Victoria Spoon Anatomic Pathology Manager Bassett Medical Center Cooperstown NY 13326 victoria.spoon@bassett.org Tel(607) 547-6357 Fax(607) 547-3203 From foreightl <@t> gmail.com Thu Oct 14 12:02:59 2010 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Thu Oct 14 12:03:06 2010 Subject: [Histonet] Molecular & FISH technologist needed in Seattle, WA In-Reply-To: References: Message-ID: *Molecular & FISH technologist* * * *SUMMARY* Under the supervision of the Histology Supervisor the Molecular & FISH Technologist performs the tests and procedures assigned to the Molecular Pathology Department. The position is responsible for specimen accessioning, preservation, processing, testing, reagent preparation, quality assurance, equipment maintenance, operation, reading of slides, initial report preparation and communication with clients and pathologists and all other duties assigned by the Supervisor. * * *ESSENTIAL FUNCTIONS* ? Performs all routine and complex special molecular and FISH procedures with the understanding of molecular diseases, cellular and tissue structures, techniques, principles, theory and instrumentation. ? With general direction from the Medical Director and the Molecular consultant is responsible for set up and validation of new FISH procedures. ? Assures specimen integrity, labeling, and preservation requirements are met and gathers necessary clinical information as needed. ? Fully responsible for operation, troubleshooting and maintenance of the molecular and FISH related equipment in the department. Completes all instrument function verification, maintenance, and documents according to procedure in department. Ensure that equipment defects and malfunctions are reported and repaired. ? Demonstrates general knowledge of pathological and physiological conditions that affect test results and tissue staining. ? Uses and documents controls, proficiency test samples and maintains quality assurance records appropriately. Monitors quality control results and takes immediate and proper action when controls are unacceptable. Follow defined procedures with only supervisor or pathologist approved modifications or deviations. ? Recognizes and troubleshoots routine and complex problems and assist other department staff and lab assistants with technical problems. ? Responsible for the proper handling and disposal of all biohazardous materials and chemically hazardous materials including the neutralization or recycling of chemicals before disposal or reuse. Always maintains a safe work environment and attends all safety training classes and conforms to all company safety guidelines and requirements. ? Takes on additional responsibility for training Laboratory personnel including Laboratory Assistants, NRT?s and other Histotechs in molecular and FISH procedures. ? Will be responsible for writing/updating Operating Procedures. ? Assists supervisory staff in monitoring workflow and insuring that work priorities are met for the department. ? Participates in special projects or other duties as assigned by supervisor staff or company. ? Always maintains a pleasant, courteous attitude when answering the telephone. ? Participates in weekend and holiday schedules as staffing requirements dictate. Remains flexible and works a share of overtime or different shifts if necessary during staffing shortages or emergencies. ? Participates in continuing education classes and courses. Strongly encouraged to keep updated on recent advances in the field of FISH and to take at least 10 credit hours of continuing education a year. ? Conforms to established and new procedures and policies instituted by the company. * * *EDUCATION and/or EXPERIENCE* ? MT (ASCP) or equivalent, Canadian or Foreign registered with college degree required. ? 3-5 years experience in molecular techniques and FISH method set up, validation and screening/reading of FISH slides required. ? Experience using automated imaging and FISH analysis software required. Please send Resume or CV to: careers@cellnetix.com Thanks, -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From katherine-walters <@t> uiowa.edu Thu Oct 14 14:10:09 2010 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Thu Oct 14 14:11:09 2010 Subject: [Histonet] phospho-Akt antibody Message-ID: Dear Histo-netters, I am trying to work up a phosphor-akt antibody (Cell Signaling #3787) for my FFPE blocks of mouse uterine tubules. Fixation is in Z-fix for 48 hours. So far I have tried HIER with both microwave retrieval and a pressure-cooker. I am using DAKO's En-vision plus rabbit secondary HRP conjugate and reacting with DAB. The results are less than impressive, although I am seeing some low level staining at 1:50. Has anyone used this antibody with better success? Thanks for any help in this matter. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf From hardin <@t> oncology.wisc.edu Thu Oct 14 15:38:27 2010 From: hardin <@t> oncology.wisc.edu (Joe Hardin) Date: Thu Oct 14 15:37:11 2010 Subject: [Histonet] viral inactivation with JB fixative Message-ID: <4CB76A43.6020201@oncology.wisc.edu> Histonetters, Does anyone know if Zinc Fixative(J B Fixative) inactivates viruses and prions? I need a reference and haven't found one in numerous literature searches. Our lab is concerned that there may still be active virus in zinc fixed tissue since it doesn't contain formalin. Thanks, From mpence <@t> grhs.net Thu Oct 14 15:57:16 2010 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Oct 14 15:57:27 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <4CB6E43E.6034.00E2.0@mont-hosp.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974A5B@is-e2k3.grhs.net> Now for someone to do the same study using FISH! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Thursday, October 14, 2010 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Ibarra JA and Rogers LW: Fixation time does not affect expression of HER2/neu. Am J Clin Pathol 2010;134:594-596. If you leave the tissue in the 70% for 36 hours, doesn't it cause the tissue to become extra hardened do to the alcohol? Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email " to your outgoing mail. >>> 10/14/2010 9:04 AM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. pathology reports (Horn, Hazel V) 2. Seeking Grossing/Histo Tech (Mighnon Lashus) 3. Re: Urinary bladder diverticula (Robert Richmond) 4. pathology reports (Tench, Bill) 5. Re: Histonet Digest, Vol 83, Issue 19 (Cathy.Crumpton@tuality.org) 6. RE: RE: New Cap Guidelines for Her2 and ER/PR (Kuhnla, Melissa) 7. RE: RE: New Cap Guidelines for Her2 and ER/PR (Bill B.) 8. RE: RE: New Cap Guidelines for Her2 and ER/PR (McMahon, Loralee A) 9. FW: How to remove Hematoxilin (Margaryan, Naira) 10. I agree with Jose, etal. (Orr, Rebecca) 11. Training labs in the San Francisco Bay Area? (Morken, Tim) 12. RE: New Cap Guidelines for Her2 and ER/PR (Amos Brooks) 13. Re: Training labs in the San Francisco Bay Area? (Jennifer MacDonald) 14. Re: IHC OOps wrong secondary (Kimberly Tuttle) 15. "2010 Focus on IHC" one day event comes to NJ (Pedro Louro) 16. Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. (Anya Salih) 17. fridge/freezer storage space (Edwards, Richard E.) 18. RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR (Patsy Ruegg) 19. RE: fridge/freezer storage space (John Shelley) ---------------------------------------------------------------------- Message: 1 Date: Wed, 13 Oct 2010 12:06:50 -0500 From: "Horn, Hazel V" Subject: [Histonet] pathology reports To: "Histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB7181249D05B@EVS1.archildrens.org> Content-Type: text/plain; charset="ISO-8859-1" When you have results from an outside lab, i.e. flow results, bone marrows, ect. How do you integrate this into your path report? It is a verbatim copy inside the report in the same format as the outside lab? Or can it be a copy with the results in a different format? A discussion has occurred within in or transcription department over this matter. We do attach the outside report to the hard copy report in our paper file. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 2 Date: Wed, 13 Oct 2010 12:21:05 -0500 From: Mighnon Lashus Subject: [Histonet] Seeking Grossing/Histo Tech To: "histonet@lists.utsouthwestern.edu" Cc: Dewayne Belew Message-ID: <197CD0B02A81F94994A285C59C8AE05C05F49D4CDB@pgnexchange.pathgroup.com> Content-Type: text/plain; charset="us-ascii" We are seeking a qualified candidate for the position of Grossing/Lead Histo Tech in our Chattanooga location. We are a state of art laboratory using the Ventana Vantage system to maintain specimen integrity; we also have Ventana special stainers and the Benchmark Ultra and XTs for our Immunohistochemistry stains. We offer a friendly working environment along with an excellent benefit package. You may obtain more information about this position at CareerBuilders.com. We also have an opening for a cytotechnologist at our Pathology office on the Erlanger Health System campus in Chattanooga, TN. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ------------------------------ Message: 3 Date: Wed, 13 Oct 2010 13:26:33 -0400 From: Robert Richmond Subject: [Histonet] Re: Urinary bladder diverticula To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Joyce Weems in Atlanta asks: >>If TUR is 88307 and resection is 88309, what CPT code do you use for >>bladder diverticuli?<< A transurethral resection of the prostate (TURP) is coded 88305 no matter how many blocks. A TUR of the bladder (presumably a TURBT, that is, a TUR of a bladder tumor) is 88307. A cystectomy specimen for cancer (resection) is 88309. There are no specific instructions for a diverticulum of the urinary bladder. Diverticula of the GI tract are however coded 88305, and I would code a urinary tract diverticulum (bladder or urethra) as 88305 also. The singular is diverticulum, the plural is diverticula. There is no such word as *diverticuli. If you don't have a copy of the anatomic pathology CPT codes, 2010 edition, I can send you a PDF of a scan of those pages. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Wed, 13 Oct 2010 10:30:24 -0700 From: "Tench, Bill" Subject: [Histonet] pathology reports To: Histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56C2@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii We scan all outside laboratory reports (including consultations, flow cytometry, special immunohistochemistry, molecular tests, etc) and insert the scanned material into an addendum which is electronically tied to the primary report. If the outside report has graphic material (ie, material that cannot be converted into a "WORD" format) it has to be excised because at least our vision of Cerner Millenium will not permit non-text material to be inserted into the report document (We use an Epson scanner and Epson program that does a very handy job of converting PDF documents into WORD documents and allows for the excision of non-WORD material---like fancy headers). When appropriate, ie, it is not obvious what the results mean, we may add our own comment about how these results should be interpreted in the setting of our other material. This saves a lot of transcription (which use to be the way we accomplished this) and avoids transcriptional errors. It generally allows capture of the name and address of the outside laboratory, which is a CLIA requirement. Incorporating the results of special tests into the report is a CAP requirement. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 5 Date: Wed, 13 Oct 2010 10:40:39 -0700 From: Cathy.Crumpton@tuality.org Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 19 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" We are not open on weekends and worked out a deal with our core l that is open 24/7. When we have a breast on the processer we will run a Sat. program that ends at 21:00. They added a task on their dai They j were being embed the embedding cent embedding. No harm Cathy Tuality Community Hospital Hillsbo (503)681-1292 ------------------------------ Message: 6 Date: Wed, 13 Oct 2010 13:51:45 -0400 From: "Kuhnla, Melissa" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Mike Pence , Phyllis Thaxton , "Mahoney,Janice A" , Joyce Cline , Message-ID: Content-Type: text/plain; charset="US-ASCII" Fixation prior to the processor will not exce4ed 12 hrs. That is why we program the processor for 36...not to exceed 48. ________________________________ From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, October 13, 2010 11:04 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than "normal processing" time in 70% (ie. more than 1 hr). -----Original Message----- From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% ________________________________ From: Phyllis Thaxton [mailto:dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Kuhnla, Melissa" To: "Mahoney,Janice A" ; Mike Pence ; Joyce Cline ; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.souders@wchsys.org ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ------------------------------ Message: 7 Date: Wed, 13 Oct 2010 14:12:59 -0500 From: "Bill B." Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Message-ID: Content-Type: text/plain; charset="us-ascii" How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why >we program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the >processor? Your total time in formalin can not exceed 48 hr. And you >will still need to validate your process if you hold your tissue longer >than "normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > For the weekend, we have our processor set for 36 hours in formalin >and then a hold in 70%. This allows for complete fixation and cuts down >on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the tissues >are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order >to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > Formalin fixation time does not impact the results of FISH as it does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time is >still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org ------------------------------ Message: 8 Date: Wed, 13 Oct 2010 15:23:10 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Bill B." , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" If our cases are needle cores they are almost immediately put into formalin from the patient. That time is recorded by the nurse or the clinician that took the specimen. The larger breast samples are received fresh from the OR and are immediately grossed either by a PA or resident. Those samples are examined, sliced (to expose the surface area of the specimen) and placed into formalin. That is when the fixation time is recorded for the larger specimens. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. [bill501@mindspring.com] Sent: Wednesday, October 13, 2010 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why >we program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpence@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the >processor? Your total time in formalin can not exceed 48 hr. And you >will still need to validate your process if you hold your tissue longer >than "normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:Melissa.Kuhnla@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dchihc@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" > To: "Mahoney,Janice A" ; Mike Pence >; Joyce Cline ; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.souders@wchsys.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 13 Oct 2010 16:03:28 -0500 From: "Margaryan, Naira" Subject: [Histonet] FW: How to remove Hematoxilin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histoworld, I would like to repeat my staining on the slides already coverslipped but need to remove hematoxilin first. How to remove hematoxilin? Is it need to repeat Antigen retrieval? Thanks in advance, Naira ------------------------------ Message: 10 Date: Wed, 13 Oct 2010 16:29:49 -0500 From: "Orr, Rebecca" Subject: [Histonet] I agree with Jose, etal. To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Thanks for your response, Jose, I agree with your points. I see these guidelines as the first attempt to standardize something that has numerous variables. How can we expect to have the manufacturers of these markers offer (or be required by the FDA) consistent results when our end of this process is "all over the board"? An ER, PR Her2 that we both buy from the same manufacturer should work the exact same way in my lab as it does in yours, IF we both process in the exact same way. I doubt very much if histology and IHC testing will ever be as exact as a clinical chemistry assay for example, but it's a start. I forsee the time gap in formalin shortening as labs get used to this first step. This would mean more validation, but since we should be doing validation each year, then this may not be such a giant task. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ------------------------------ Message: 11 Date: Wed, 13 Oct 2010 15:12:54 -0700 From: "Morken, Tim" Subject: [Histonet] Training labs in the San Francisco Bay Area? To: Histonet Message-ID: <1AAF670737F193429070841C6B2ADD4C026967E880@EXMBMCB15.ucsfmedicalcenter. org> Content-Type: text/plain; charset=us-ascii To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org ------------------------------ Message: 12 Date: Wed, 13 Oct 2010 18:21:22 -0400 From: Amos Brooks Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: JEllin@yumaregional.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.l ocal > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin ------------------------------ Message: 13 Date: Wed, 13 Oct 2010 15:30:21 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Training labs in the San Francisco Bay Area? To: "Morken, Tim" Cc: Histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" With the on-line programs the training facility will need to be approved by the program. They will need to sign an affiliation agreement between the program (school) and the training site. "Morken, Tim" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/13/2010 03:17 PM To Histonet cc Subject [Histonet] Training labs in the San Francisco Bay Area? To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 13 Oct 2010 19:14:57 -0400 From: "Kimberly Tuttle" Subject: Re: [Histonet] IHC OOps wrong secondary To: "histonet@lists.utsouthwestern.edu" , "Kimberly Tuttle" Message-ID: <4CB60536.90CE.001A.3@umm.edu> Content-Type: text/plain; charset=US-ASCII Thanks to everyone who responded. I removed the coverslip, and ran it back to water and re applied the secondary, dab and counterstain. It worked like a charm. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >>> "Kimberly Tuttle" 10/13/2010 12:07 pm >>> Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 15 Date: Wed, 13 Oct 2010 20:34:17 -0400 From: Pedro Louro Subject: [Histonet] "2010 Focus on IHC" one day event comes to NJ To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The date is set, the topics are in place, and the money is just right, all we need....is you to attend. When: Friday, November 5, 2010 Where: Somerset Medical Center 110 Rehill Ave Somerville, NJ 08876 *"2010 Focus on IHC"* presented by NJSH and sponsored by BioCare Medical, LLC $10.00(members) $40.00 (Non-members) gets you: - 6 CEU's - Breakfast, Lunch and Snacks - Great learning experience Hope to see you there, Pedro Louro President (New Jersey Society for Histotechnology) Co-chair Membership Committee ________________________________________________________________________ ___ *Meeting Schedule * 7:30-8:30AM Registration and Continental Breakfast * 8:30-12:00 *AM Session with Coffee Break * Seminars * Mouse Models and IHC; Linda Dean Antibodies 2010; Fatima Natar * OR ** Wet Workshop * Multiplex Staining in the Anatomic Laboratory; Tara Kennedy * 12:00-1:00 *Lunch (wraps, salad, soup, dessert) * 1:00-4:30 *PM Session with Coffee Break * Seminars * CAP Regulations; Terry Murphy Validation in the IHC Laboratory; George Hoernig * OR ** Wet Workshop * Rapid In Situ Hybridization; Will Chappell ------------------------------ Message: 16 Date: Thu, 14 Oct 2010 15:34:27 +1100 From: "Anya Salih" Subject: [Histonet] Advanced workshop in 3D live cell imaging in Sydney on 16-19 November. To: Message-ID: <512D5A4F81BF054F9735F9C0AC45AB3B0281BC51@VIOLA.AD.UWS.EDU.AU> Content-Type: text/plain; charset="us-ascii" > You and your students are invited to attend the 3rd Advanced > Bio-Imaging Workshop at the University of Western Sydney on 16 - 19 > November, 2010 and the Bio-Imaging Expo (free event on 16th November > 2010) > > Tracking Molecules with Light > > Training in confocal imaging and protein 3D tracking, aggregation, > diffusion analyses. Experiments will involve mammalian cell lines, > invertebrate (coral) cells, plant and algae, bacteria and other > samples. > > Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop. > > Places limited to 35 and only 20 places left so register now. > > > Location: Confocal Bio-Imaging Facility, Building S8, Hawkesbury > Campus, University of Western Sydney > Organiser: Dr Anya Salih > Training by: Dr Salih UWS; Prof. E. Gratton and Dr M. Digman Univ. > California; Prof. Guy Cox Uni Sydney; Dr Wolfgang Becker, Becker & > Hickl GmbH Germany; Dr C Thoni Leica Microsystems, G. Symonds Zeiss) > > > Lectures and intensive hands-on training on confocal microscopes by > top researchers in the field. Learn how to explore and analyse the 3D > structural complexity of invertebrate, animal & plant cells, tissues > and micro-organisms, visualize and analyse movement of organelles and > molecules. Trial a range of novel GFP-type protein constructs. Discuss > your experiments and trial new approaches. Workshop emphasis on > advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B, > FLIM, photoactivatable fluorescent proteins. > > Invited speakers > > * Professor Enrico Gratton, Director Laboratory for Fluorescence > Dynamics, University of California, Irvine > * Professor Takeharu Nagai, Laboratory for Nanosystems > Physiology & Nikon Imaging Center, Hokkaido University Research > Institute Electronic Science, Japan > * Dr. Michelle Digman, Director Optical Biology Core Facility, > University of California, Irvine > * A/Professor Guy Cox, Australian Centre for Microscopy & > Microanalysis, University of Sydney > * Professor Leann Tilley, Department of Biochemistry, D/Director > Centre of Excellence in coherent X-ray Science, La Trobe University > * Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging > Facility, Garvan Institute of Medical Research, University of Sydney > * Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, > University of Sydney > * Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin > > Training sessions cover the following: > > Multi-colour Fluorescent proteins > Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby, > pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B > histone, actin, tubulin, Golgi, membrane); novel Photoactive > Fluorescent Proteins (EosFP, AmilRFP, kindling proteins, Phamret) from > reef corals. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein > localization & diffusion. > > Confocal Spectral Imaging > Acquisition of microspectral data (x, y, lambda) in 3D image stacks > from samples with multiple fluorescent probes or from fluorescent > coral tissues expressing a variety of GFP-type proteins (A. Salih > fluorescent corals > http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=he > alth)- Spectral unmixing, analysis, spectral FRET. > > Analysis of molecular movement and diffusion > Track proteins and other molecules in live cells. Measure protein > femtoliter concentrations. Monitor mobility and binding using > fluorescence correlation spectroscopy (FCS), scanning FCS, raster > image correlation spectroscopy (RICS), number & brightness (N&B) and > photon counting histogram (PCH). > > Fluorescence Lifetime Imaging Microscopy (FLIM) > - a powerful tool to analyse spatial distribution of excited state > lifetimes in samples: studied examples will include FRET-FLIM to study > protein interactions (e.g., DNA-Protein) in cells, imaging of > photoactivatable FPs, quenching of chlorophyll in plants, etc. > Hands-on training in FLIM and Phasor FLIM. > > > Workshop microscopes & companies > > Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto > optical beam splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B > Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging, > photon counting, FCS Nikon A1, Coherent Scientific Pty. Ltd - rapid > image acquisition, resonant scanner & 32 channel microspectral > detection Olymus FluoView FV1000 - variable barrier filter (VBF), > spectral detection, RICS and N&B > Ultra VIEW VoX spinning disc confocal microscope, high speed, > multichannel, 2D and 3D, FRAP, FLIP, photoactivation experiments, > PerkinElmer. > Confocal FLIM system, Becker & Hickl GmbH, Berlin A range of new > GFP-type protein constructs of many colours (cyan to far red) linked > to a variety of cellular proteins for in vivo protein localization > and dynamic studies, Evrogen And many more other instruments. > > > Registration > > Full workshop registration (lectures + training) will be limited to 35 > participants. Registration will be on a first come first served basis. > > Contact Anya Salih a.salih@uws.edu.au to reserve your workshop place > Students $650 (GST inclusive) > All other $850 (GST inclusive) > > > Attendance of the BioImaging Expo on 16th November does not require > registration by please rsvp Pamela McMurtry > [pamela.mcmurtry@theconferenceteam.com.au] > Accommodation details at registration website, from $260 per week per > single room at UWS College. Bus shuttle will be available between > Sydney airport and the workshop venue at UWS campus. > > Kind regards, Anya Dr Anya Salih Confocal Bio-Imaging Facility University Western Sydney Australia > 61 2 45701452 > a.salih@uws.edu.au ------------------------------ Message: 17 Date: Thu, 14 Oct 2010 12:07:46 +0100 From: "Edwards, Richard E." Subject: [Histonet] fridge/freezer storage space To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. ------------------------------ Message: 18 Date: Thu, 14 Oct 2010 06:49:54 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "'Amos Brooks'" , , Message-ID: <26E0799EA0D8454A86A3F86DF8A6F22D@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" Here here Amos. This is why I am for using multi tissue controls that have considered a range for most of the differences in tissues, fixation and processing encountered. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, October 13, 2010 4:21 PM To: JEllin@yumaregional.org; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Hi, I'm going to have to disagree with this approach. Lumping all specimens into the same procedure (fixation/processing time) is in no way a step toward individualized care that is so often discussed. Doing this ignores the basic fundamental differences between the specimens. A liver is different from a breast and a brain. Likewise a particularly fatty breast is different from a fiberous one, or one that is cut smaller than the one the other pathologist (or PA) stuffed into a cassette. Each of these situations needs to be addressed differently from fixation to processing times and to be entirely honest staining times in many cases. I fear you may be oversimplifying the situation and calling it standardization. All the best, Amos Date: Wed, 13 Oct 2010 08:05:27 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: "Kuhnla, Melissa" , "Phyllis Thaxton" , "Mahoney,Janice A" < Janice.Mahoney@alegent.org>, "Mike Pence" , "Joyce Cline" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C68C9@EXCHANGECLUSTER.yumaregional.l ocal > Content-Type: text/plain; charset="US-ASCII" OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 14 Oct 2010 08:58:25 -0400 From: John Shelley Subject: [Histonet] RE: fridge/freezer storage space To: "Edwards, Richard E." , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Richard, We are a research facility and have the same issue with not always knowing where and whose stuff is in the freezer or refrigerators. Even with the best system though you still have to have people who communicate that they are taking stuff out and also replacing back where it was removed from. We have instituted the use of a system called Freezer Pro. Here is the web address http://www.ruro.com/products/freezerpro.html At least this is a start, hope it helps. Kind Regards! John J Shelley Senior Research Associate, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Thursday, October 14, 2010 7:08 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] fridge/freezer storage space Does anyone out there have an effective policy for monitoring the storage of material at 4C,-20C or -80C. At the moment if any more space is required the solution is usually to buy another fridge or freezer, which then takes up more floor space, uses more electricity and quickly fills as individuals use the space that has become available. So can anyone suggest/ or has in place a system whereby the contents of fridges/freezers can be monitored on a regular basis and old/out of date stuff is disposed off. I expect that all you GLP laboratories have it sorted, and I would welcome any input from you. Ours is a university lab where no such rules/regulations seem to exist and often when people leave they forget to throw out their stuff which can then remain for years. Many thanks Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 83, Issue 20 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From k84as <@t> yahoo.com Thu Oct 14 16:03:30 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Thu Oct 14 16:03:34 2010 Subject: [Histonet] alkaline phosphatase Message-ID: <336633.16621.qm@web112611.mail.gq1.yahoo.com> ?dear all i'm going to detect alkaline phsphatase in avian intestine and i didn't do that befor so any help with protocol?? or kits for that and how to?use?it can we detect it in FFPT? ? by the way i have sent a question twice befor about?how to ?diff.?between red muscle and white one and have no response!! any help please ? thanx From histologyinfo <@t> gmail.com Thu Oct 14 16:31:19 2010 From: histologyinfo <@t> gmail.com (Pedro Louro) Date: Thu Oct 14 16:31:23 2010 Subject: [Histonet] 2010 Focus on IHC comes to NJ Message-ID: The date is set, the topics are in place, and the money is just right, all we need....is you to attend. When: Friday, November 5, 2010 Where: Somerset Medical Center 110 Rehill Ave Somerville, NJ 08876 "2010 Focus on IHC" presented by NJSH and sponsored by BioCare Medical, LLC $10.00(members) $40.00 (Non-members) gets you: - 6 CEU's - Breakfast, Lunch and Snacks - Great learning experience Hope to see you there, Pedro Louro President (New Jersey Society for Histotechnology) Co-chair Membership Committee ___________________________________________________________________________ Meeting Schedule 7:30-8:30AM Registration and Continental Breakfast 8:30-12:00 AM Session with Coffee Break Seminars Mouse Models and IHC; Linda Dean Antibodies 2010; Fatima Natar OR Wet Workshop Multiplex Staining in the Anatomic Laboratory; Tara Kennedy 12:00-1:00 Lunch (wraps, salad, soup, dessert) 1:00-4:30 PM Session with Coffee Break Seminars CAP Regulations; Terry Murphy Validation in the IHC Laboratory; George Hoernig OR Wet Workshop Rapid In Situ Hybridization; Will Chappell From patrick.lewis <@t> seattlechildrens.org Thu Oct 14 18:11:59 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Thu Oct 14 18:12:08 2010 Subject: [Histonet] I have a couple of quick questions Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C05F5A165@s107.childrens.sea.kids> If I want to remove a cover slip that is coversliped with permount. Do I need to use xylene to get rid of the semi hardened permount gunk? Also, If I am heating paraffin sectioned slides overnight at 37C to adhere them what happens if I over cook them by leaving them at 37C for more than one day? Say 3-4 days at 37C It probably wont hurt them as they haven't been epitope retrieved yet. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From godsgalnow <@t> aol.com Thu Oct 14 19:22:14 2010 From: godsgalnow <@t> aol.com (=?utf-8?B?Z29kc2dhbG5vd0Bhb2wuY29t?=) Date: Thu Oct 14 19:22:15 2010 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gSSBoYXZlIGEgY291cGxlIG9mIHF1aWNrIHF1ZXN0aW9ucw==?= Message-ID: <201010150021.o9F0Lxae002290@imr-ma05.mx.aol.com> Yes, soak slides in xylene to remove coverslip. A few days in 37 degree oven won't hurt Roxanne Sent from my Verizon Wireless Phone ----- Reply message ----- From: "Lewis, Patrick" Date: Thu, Oct 14, 2010 7:11 pm Subject: [Histonet] I have a couple of quick questions To: If I want to remove a cover slip that is coversliped with permount. Do I need to use xylene to get rid of the semi hardened permount gunk? Also, If I am heating paraffin sectioned slides overnight at 37C to adhere them what happens if I over cook them by leaving them at 37C for more than one day? Say 3-4 days at 37C It probably wont hurt them as they haven't been epitope retrieved yet. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Fri Oct 15 02:51:07 2010 From: member <@t> linkedin.com (Tyrone Genade via LinkedIn) Date: Fri Oct 15 02:51:15 2010 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <490406874.6140284.1287129067178.JavaMail.app@ech3-cdn08.prod> LinkedIn ------------Tyrone Genade requested to add you as a connection on LinkedIn: ------------------------------------------ Jackie, I'd like to add you to my professional network on LinkedIn. - Tyrone Accept invitation from Tyrone Genade http://www.linkedin.com/e/yvpgd1-gfarewfb-54/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I901563316_3/pmpxnSRJrSdvj4R5fnhv9ClRsDgZp6lQs6lzoQ5AomZIpn8_cRYScjcPdzkNc3B9bRATu3tIkSBibPgUejcUdjcUdP4LrCBxbOYWrSlI/EML_comm_afe/ View invitation from Tyrone Genade http://www.linkedin.com/e/yvpgd1-gfarewfb-54/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I901563316_3/0PnPoNcPcSdj4MekALqnpPbOYWrSlI/svi/ ------------------------------------------ DID YOU KNOW you can conduct a more credible and powerful reference check using LinkedIn? Enter the company name and years of employment or the prospective employee to find their colleagues that are also in your network. This provides you with a more balanced set of feedback to evaluate that new hire. http://www.linkedin.com/e/yvpgd1-gfarewfb-54/rsr/inv-27/ -- (c) 2010, LinkedIn Corporation From BSullivan <@t> shorememorial.org Fri Oct 15 06:09:14 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Fri Oct 15 06:12:05 2010 Subject: [Histonet] I have a couple of quick questions In-Reply-To: <7EA5752B2903B143A5B845DEA87D5D1C05F5A165@s107.childrens.sea.kids> Message-ID: Yes..... to remove mounting media you need to leave them in the Xylene after the coverslip is removed. If the mounting media is not totally removed your stain, whatever one you are doing, will not give you the results you need. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Lewis, Patrick" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] I have a couple of quick questions 10/14/2010 07:11 PM If I want to remove a cover slip that is coversliped with permount. Do I need to use xylene to get rid of the semi hardened permount gunk? Also, If I am heating paraffin sectioned slides overnight at 37C to adhere them what happens if I over cook them by leaving them at 37C for more than one day? Say 3-4 days at 37C It probably wont hurt them as they haven't been epitope retrieved yet. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 15 08:31:48 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 15 08:31:53 2010 Subject: [Histonet] I have a couple of quick questions In-Reply-To: <7EA5752B2903B143A5B845DEA87D5D1C05F5A165@s107.childrens.sea.kids> Message-ID: <906664.81043.qm@web65713.mail.ac4.yahoo.com> Yes, xylene is the way to go. No, 37?C will not hurt the sections no matter for how long they are at that temp. Ren? J. --- On Thu, 10/14/10, Lewis, Patrick wrote: From: Lewis, Patrick Subject: [Histonet] I have a couple of quick questions To: Histonet@lists.utsouthwestern.edu Date: Thursday, October 14, 2010, 7:11 PM If I want to remove a cover slip that is coversliped with permount.? Do I need to use xylene to get rid of the semi hardened permount gunk? Also,? If I am heating paraffin sectioned slides overnight at 37C to adhere them what happens if I over cook them by leaving them at 37C for more than one day? Say 3-4 days at 37C? It probably wont hurt them as they haven't been epitope retrieved yet. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115? OFFICE 000-000-0000? PAGER 000-000-0000? CELL 206-884-7311? FAX patrick.lewis@seattlechildrens.org OFFICE? 1900 9th Avenue Seattle, WA 98101 MAIL? ? ? M/S C9S-8, Seattle, WA 98101 WWW? ???seattlechildrens.org CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.? Any unauthorized review, use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Fri Oct 15 08:53:42 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 15 08:53:47 2010 Subject: [Histonet] Immunohistochemistry images Message-ID: <20101015065342.9e2d9aa830e8449a2412eb1e4f2f067e.900cf55e04.wbe@email04.secureserver.net> Are you wanting to save your specific images, or just need a refe rence guide for QC? Photobucket or Snapfish are two sites that are pr Sarah Goebel, B.A., HT (ASCP) Histotec XBiotech USA Inc. < 8201 East Riverside Austin, Texas 78744 ( -------- Original Message -------- Subject: RE: [Histonet] Immunohistochemistry images From: "Blazek, Linda" <[1]lblazek@digestivespecialists.com> Date: Thu, October 14, 2010 9:04 am To: 'Michele Carr' <[2]micheleca "[3]histonet@lists.utsout histonet@lists.utsouthwestern.edu> Try the web site of the vender that you get your antibodies from. -----Original Message----- From: [5]histonet [[6]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Carr Sent: Thursday, October 14, 2010 11:41 AM To: [7]histonet@lists.uts Subject: [Histonet] Immunohistochemistry images Hi everyone, I was wondering if anyone knew of a website that I can view an save the IHC images. We are putting together a new procedure manual a pathogists want images of the antibodies we use to be included in the manua Thanks in advance for your responses. Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouth [9]http: _______________________________________________ Histonet mailing list [10]Histonet@lists.utsouth [11]http: References 1. 3D"mailto:lblazek@digestivespecialists.co 2. 3D"mailto:michelecarr10@yahoo.com" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:histonet-bounces@l 7. 3D"mailto:histonet@lists.utsouthwestern.edu" 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 10. 3D"mailto:Histonet@lists.utsouthwestern.edu" 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From histotech <@t> imagesbyhopper.com Fri Oct 15 08:59:27 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Oct 15 09:00:06 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16403987E6200@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E16403987E61CD@CHEXCMS10.one.ads.che.org> <661949901A768E4F9CC16D8AF8F2838C03974A57@is-e2k3.grhs.net> <92AD9B20A6C38C4587A9FEBE3A30E16403987E6200@CHEXCMS10.one.ads.che.org> Message-ID: Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" wrote: > > CMS/NCCI Update Dated October 1, 2009 > > 8. The unit of service for special stains (CPT codes 88312-88313) and > immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If > it is medically reasonable and necessary to perform the same stain on > more than one specimen or more than one block of tissue from the same > specimen, additional units of service may be reported for the > additional specimen(s) or block(s). Physicians should not report more > than one unit of service for a stain performed on a single tissue > block. For example it is common practice to cut multiple levels from a > tissue block and stain each level with the same stain. The multiple > levels from the same block of tissue stained with the same stain > should not be reported as additional units of service. Only one unit > of service should be reported for the stain on multiple levels from > the single tissue block. Additionally, controls performed with special > stains should not be reported as separate units of service for the > stain. > > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Thursday, October 14, 2010 11:31 > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Can you site your source, please. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce > Sent: Thursday, October 14, 2010 10:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > > > > The change is that you can bill per block now and not per specimen. This is for immunos and special stains. It does make a huge difference! > > Best, > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish > Sent: Thursday, October 14, 2010 11:10 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. > > Thanks, > Chris Evanish > Montgomery Hospital > Norristown PA > > Chris D. Evanish > Histology Supervisor > Montgomery Hospital > 610-270-2379 > > Please consider the environment before printing this email " to your outgoing mail. > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dchihc <@t> yahoo.com Fri Oct 15 09:05:22 2010 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Fri Oct 15 09:05:32 2010 Subject: [Histonet] Optimal processing for prostate needle biopsies In-Reply-To: References: , <760058.38095.qm@web43509.mail.sp1.yahoo.com> Message-ID: <785089.10425.qm@web43510.mail.sp1.yahoo.com> Nilesh, ?This doesn't sound like a staining problem, it sounds like a processing problem. Try preprocessing for 5 minutes instead of 15 and see if that helps.?OR what we had to do with GI biopsies, we stopped using any preprocessing with them. After fixation, they are placed in molecular fixative for 10 minutes before processing. ? If that doesn't work, I would investigate further?to where the biopsy was actually obtained. We had a problem with urologists obtaining the core, then swishing the needle gun around in a container of saline, then ADDING formalin to the saline after the biopsy was obtained. The biopsies?were horrible. ? Hope this helps. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Gupta, Nilesh" To: Phyllis Thaxton ; "histonet@lists.utsouthwestern.edu" Sent: Thu, October 14, 2010 8:08:17 AM Subject: RE: [Histonet] Optimal processing for prostate needle biopsies Phyllis, We hold all our needle cores overnight and pre-process for 15 min. The problems we are having is shrunken nuclei and nucleoli are not?distinct?even in carcinoma cases. Cracking artifacts of cores (longitudinal), darker and paler staining along the length of the cores. We tried Harris but staining with Mayer's brings out nucleoli better. ? ? ________________________________ From: Phyllis Thaxton [dchihc@yahoo.com] Sent: Wednesday, October 13, 2010 10:37 AM To: Gupta, Nilesh; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Optimal processing for prostate needle biopsies How?long are you pre-processing the prostate biopsies before processing?on the XPress120? We use the XPress50 we?fix the biopsies in Hollandes for 2 hours, then wash for 20 minutes, pre-processing solution for 10 minutes then process. We use Harris Hematoxylin?in our H&E and morphology is good. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Gupta, Nilesh" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, October 12, 2010 12:02:23 PM Subject: [Histonet] Optimal processing for prostate needle biopsies I have a few questions regrading processing of prostate needle biopsies. 1.? What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2.? Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3.? What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. 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If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From bakevictoria <@t> gmail.com Fri Oct 15 09:25:34 2010 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Oct 15 09:25:44 2010 Subject: [Histonet] negative controls Message-ID: Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki From BSullivan <@t> shorememorial.org Fri Oct 15 09:37:55 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Fri Oct 15 09:40:43 2010 Subject: [Histonet] negative controls In-Reply-To: Message-ID: To my knowledge for this stain to be legitimate and meet criteria you must run your positive and negative controls on the same run. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Victoria Baker To Sent by: Histo Net list server histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] negative controls 10/15/2010 10:25 AM Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Oct 15 09:45:14 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Oct 15 09:45:21 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E16403987E61CD@CHEXCMS10.one.ads.che.org> <661949901A768E4F9CC16D8AF8F2838C03974A57@is-e2k3.grhs.net> <92AD9B20A6C38C4587A9FEBE3A30E16403987E6200@CHEXCMS10.one.ads.che.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403A6810936@CHEXCMS10.one.ads.che.org> http://www.flpath.org/rli2.asp Here is a link that will help.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" wrote: > > CMS/NCCI Update Dated October 1, 2009 > > 8. The unit of service for special stains (CPT codes 88312-88313) and > immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If > it is medically reasonable and necessary to perform the same stain on > more than one specimen or more than one block of tissue from the same > specimen, additional units of service may be reported for the > additional specimen(s) or block(s). Physicians should not report more > than one unit of service for a stain performed on a single tissue > block. For example it is common practice to cut multiple levels from a > tissue block and stain each level with the same stain. The multiple > levels from the same block of tissue stained with the same stain > should not be reported as additional units of service. Only one unit > of service should be reported for the stain on multiple levels from > the single tissue block. Additionally, controls performed with special > stains should not be reported as separate units of service for the > stain. > > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Thursday, October 14, 2010 11:31 > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Can you site your source, please. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Thursday, October 14, 2010 10:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > > > > The change is that you can bill per block now and not per specimen. This is for immunos and special stains. It does make a huge difference! > > Best, > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris > Evanish > Sent: Thursday, October 14, 2010 11:10 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. > > Thanks, > Chris Evanish > Montgomery Hospital > Norristown PA > > Chris D. Evanish > Histology Supervisor > Montgomery Hospital > 610-270-2379 > > Please consider the environment before printing this email " to your outgoing mail. > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sgoebel <@t> xbiotech.com Fri Oct 15 09:52:08 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 15 09:52:13 2010 Subject: [Histonet] negative controls Message-ID: <20101015075208.9e2d9aa830e8449a2412eb1e4f2f067e.a92881fa6c.wbe@email04.secureserver.net> Pretty sure you have to run your positive with your negative to keep the conditions 100% the same. You don't have to put them on the same slide necessarily, but they need to be on the same run. Also, say you are running 15 HP patient slides, you can have one negative control for all of these as long as it is on the same run. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] negative controls From: Victoria Baker Date: Fri, October 15, 2010 7:25 am To: Histo Net list server Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WBRID <@t> capefearvalley.com Fri Oct 15 09:52:49 2010 From: WBRID <@t> capefearvalley.com (Wendy Bridges) Date: Fri Oct 15 09:52:55 2010 Subject: [Histonet] FT HT needed in Fayetteville, NC Message-ID: We're more than a job...we're a family. Cape Fear Valley Health is a regional health system serving a six-county region of Southeastern North Carolina, with more than 935,000 patient visits annually. A private not-for-profit organization and the state's ninth largest health system, it includes: Cape Fear Valley Medical Center, Highsmith-Rainey Specialty Hospital, Cape Fear Valley Rehabilitation Center, Behavioral Health Care and Bladen County Hospital. We are centrally located, in a diverse community of 300,000 residents, just two hours from North Carolina's pristine beaches and four hours from the majestic Great Smoky Mountains! Excellent Benefits!! To learn more or to talk with a recruiter, call 877-7-CAPEFEAR or you can apply online at www.capefearcareers.com . * We're looking for HISTOTECHNOLOGIST/HISTOTECHNICIAN to work in our busy, state-of-the art CAP accredited laboratory. Bachelor or Associate degree in relevant science and appropriate clinical training in the testing of neonatal, pediatric, adolescent and adult patients. HTL (ASCP)/HT (ASCP) or equivalent registration. 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From marktarango <@t> gmail.com Fri Oct 15 10:02:44 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Oct 15 10:02:50 2010 Subject: [Histonet] negative controls In-Reply-To: References: Message-ID: Hi Vikki, It's best to run it all together and run a negative control for each detection kit. Mark On Fri, Oct 15, 2010 at 7:25 AM, Victoria Baker wrote: > Hi > I have a hypothetical question to those who run IHC on Ventana instruments. > Are you running your negatives with your patient/test cases or on a > separate > run? Also, if you are doing this and have to use a different detection kit > how do you work the QA/QC portion of this for CAP requirements. > > Thanks > > Vikki > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LSebree <@t> uwhealth.org Fri Oct 15 10:08:28 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Oct 15 10:08:34 2010 Subject: [Histonet] negative controls In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E273839A04D@UWHC-MAIL01.uwhis.hosp.wisc.edu> We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Fri Oct 15 10:17:53 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 15 10:17:58 2010 Subject: [Histonet] negative controls Message-ID: <20101015081753.9e2d9aa830e8449a2412eb1e4f2f067e.0dbca7bc5b.wbe@email04.secureserver.net> Why do you need a negative control for each block if you are runn the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co slides you d that control? Sarah Goebel, B.A., HT (ASCP) Histotechnician< XBiotech USA Inc. 8201 East Riverside Dr. Bld Austin, Texas 78744 (512)386- -------- Original Message -------- Subject: RE: [Histonet] negative controls From: "Sebree Linda A" <[1]LSebree@ Date: Fri, October 15, 2010 8:08 am To: "Victoria Baker" <[2]bakevict server" <[3]HistoNet@lists.uts We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [4]histonet [[5]mailto:histon Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list [6]Histonet@lists.utsouth [7]http: _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouth [9]http: References 1. 3D"mailto:LSebree@uwhealth.org" 2. 3D"mailto:bakevictoria@gmail.com" 3. 3D"mailto:HistoNet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From rjbuesa <@t> yahoo.com Fri Oct 15 10:33:37 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 15 10:40:22 2010 Subject: [Histonet] negative controls In-Reply-To: <20101015081753.9e2d9aa830e8449a2412eb1e4f2f067e.0dbca7bc5b.wbe@email04.secureserver.net> Message-ID: <276458.11208.qm@web65714.mail.ac4.yahoo.com> Because each tissue block?has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can?you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. Ren? J.? --- On Fri, 10/15/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: RE: [Histonet] negative controls To: "Sebree Linda A" Cc: "Histo Net list server" Date: Friday, October 15, 2010, 11:17 AM ???Why do you need a negative control for each block if you are runn= ing ???the? same? antibody? on each patient block?? Is it just for case by c???ase? reference? so? the negative is filed with the patient slide?? Why ???co=? uldn't? you? have? a control slide bank that was dated so all the ???slides you d= id on that day, on that run, could be referenced back to ???that control? = ; Just curious? ???Sarah Goebel, B.A., HT (ASCP) ???Histotechnician<= br> ???XBiotech USA Inc. ???8201 East Riverside Dr. Bld= g 4 Suite 100 ???Austin, Texas? 78744 ???= ???(512)386-= 5107 ???-------- Original Message -------- ???Subject: RE: [Histonet] negative controls ???From: "Sebree Linda A" <[1]LSebree@= uwhealth.org> ???Date: Fri, October 15, 2010 8:08 am ???To:? "Victoria? Baker"? <[2]bakevict= oria@gmail.com>, "Histo Net list ???server" ???<[3]HistoNet@lists.uts= outhwestern.edu> ???We run negative controls on every block of a case within the same run. ???On autopsy cases, we only run 1 negative per tissue type, within the ???same run...this is the only exception to the rule of 1 negative per ???block. ???Linda A. Sebree ???University of Wisconsin Hospital & Clinics ???IHC/ISH Laboratory ???DB1-223 VAH ???600 Highland Ave. ???Madison, WI 53792 ???(608)265-6596 ???-----Original Message----- ???From: [4]histonet= -bounces@lists.utsouthwestern.edu ???[[5]mailto:histon=? et-bounces@lists.utsouthwestern.edu]? On Behalf Of ???Victoria ???Baker ???Sent: Friday, October 15, 2010 9:26 AM ???To: Histo Net list server ???Subject: [Histonet] negative controls ???Hi ???I have a hypothetical question to those who run IHC on Ventana ???instruments. ???Are you running your negatives with your patient/test cases or on a ???separate ???run? Also, if you are doing this and have to use a different detection ???kit ???how do you work the QA/QC portion of this for CAP requirements. ???Thanks ???Vikki ???_______________________________________________ ???Histonet mailing list ???[6]Histonet@lists.utsouth= western.edu ???[7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet ???_______________________________________________ ???Histonet mailing list ???[8]Histonet@lists.utsouth= western.edu ???[9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References ???1. 3D"mailto:LSebree@uwhealth.org" ???2. 3D"mailto:bakevictoria@gmail.com" ???3. 3D"mailto:HistoNet@lists.utsouthwestern.edu" ???4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" ???5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" ???6. 3D"mailto:Histonet@lists.utsouthwestern.edu" ???7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ???8. 3D"mailto:Histonet@lists.utsouthwestern.edu" ???9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Fri Oct 15 10:47:50 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 15 10:47:56 2010 Subject: [Histonet] negative controls Message-ID: <20101015084750.9e2d9aa830e8449a2412eb1e4f2f067e.1f487b8a54.wbe@email04.secureserver.net> So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: Rene J Buesa Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A , sgoebel@xbiotech.com Cc: Histo Net list server Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. Ren? J. --- On Fri, 10/15/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: RE: [Histonet] negative controls To: "Sebree Linda A" Cc: "Histo Net list server" Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician<= br> XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744 = (512)386-= 5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: "Sebree Linda A" <[1]LSebree@= uwhealth.org> Date: Fri, October 15, 2010 8:08 am To: "Victoria Baker" <[2]bakevict= oria@gmail.com>, "Histo Net list server" <[3]HistoNet@lists.uts= outhwestern.edu> We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [4]histonet= -bounces@lists.utsouthwestern.edu [[5]mailto:histon= et-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list [6]Histonet@lists.utsouth= western.edu [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouth= western.edu [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:LSebree@uwhealth.org" 2. 3D"mailto:bakevictoria@gmail.com" 3. 3D"mailto:HistoNet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Oct 15 10:56:57 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Oct 15 10:57:06 2010 Subject: [Histonet] negative controls In-Reply-To: <20101015084750.9e2d9aa830e8449a2412eb1e4f2f067e.1f487b8a54.wbe@email04.secureserver.net> References: <20101015084750.9e2d9aa830e8449a2412eb1e4f2f067e.1f487b8a54.wbe@email04.secureserver.net> Message-ID: Hi Sarah, It's better to have the control on the same slide. There are slides that work and slides that don't. You know which ones are good and which ones are bad because you have that control on each slide. It's not always a complete run that fails. Yes, you CAN have a single batch control (it's not against the rules), but best practice is to have a control on each slide. You don't process a control for each case, you use a similarly processed piece of positive tissue placed on each slide (except the negative which should just have the patient tissue). Mark On Fri, Oct 15, 2010 at 8:47 AM, wrote: > So for every HP you do, you process a control cassette with the patient > tissue cassette? That seems like alot? How do you get that many > control tissues on a daily basis? What do you do with the remaining > tissue in the control block? If you throw them away everyday, I would > be interested in some of them. How do you know what IHC stains the > pathologist is going to order to know what control tissue to fix and > process at the exact same time? We have always just had a bunch of > blocks that you cut a control from? I understand that there is > variability with processing, age, etc. not trying to be dense just still > don't understand... Most places I have ever worked have control blocks > that they cut a fresh control from everyday, then stain with the patient > tissue. If there are 3 HP cases, from what I am understanding, you guys > are saying you need 3 controls for slides that are on the same machine, > with the same reagents, same antibody, and same times. Why couldn't you > just have one for all 3 cases? Then the next day have a fresh ONE for > that day, date them, and file them. So if you needed to see the HP > control for October 15th, you could go pull the control for that day... > > Sarah Goebel, B.A., HT (ASCP) > Histotechnician > > > XBiotech USA Inc. > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas 78744 > > (512)386-5107 > > > > > -------- Original Message -------- > Subject: RE: [Histonet] negative controls > From: Rene J Buesa > Date: Fri, October 15, 2010 8:33 am > To: Sebree Linda A , sgoebel@xbiotech.com > Cc: Histo Net list server > > Because each tissue block has its own characteristics regarding fixation > and processing some of which can influence the reactivity. If you have a > bank of negative controls, how can you be sure that any of those blocks > have received exactly the same treatment and reacted in the same way to > the test block? > The same goes for any bank of positives, so that is why you should have > a positive control section in the same slide as the test section. > Ren? J. > > --- On Fri, 10/15/10, sgoebel@xbiotech.com wrote: > > > From: sgoebel@xbiotech.com > Subject: RE: [Histonet] negative controls > To: "Sebree Linda A" > Cc: "Histo Net list server" > Date: Friday, October 15, 2010, 11:17 AM > > > Why do you need a negative control for each block if you are runn= > ing > the same antibody on each patient block? Is it just for case by c > ase reference so the negative is filed with the patient slide? Why > co= uldn't you have a control slide bank that was dated so all > the > slides you d= id on that day, on that run, could be referenced back > to > that control? = ; Just curious? > > Sarah Goebel, B.A., HT (ASCP) > > Histotechnician<= br> > > XBiotech USA Inc. > > 8201 East Riverside Dr. Bld= g 4 Suite 100 > > Austin, Texas 78744 > > = > > (512)386-= 5107 > > -------- Original Message -------- > Subject: RE: [Histonet] negative controls > From: "Sebree Linda A" <[1]LSebree@= uwhealth.org> > Date: Fri, October 15, 2010 8:08 am > To: "Victoria Baker" <[2]bakevict= oria@gmail.com>, "Histo Net > list > server" > <[3]HistoNet@lists.uts= outhwestern.edu> > We run negative controls on every block of a case within the same > run. > On autopsy cases, we only run 1 negative per tissue type, within the > same run...this is the only exception to the rule of 1 negative per > block. > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > -----Original Message----- > From: [4]histonet= -bounces@lists.utsouthwestern.edu > [[5]mailto:histon= et-bounces@lists.utsouthwestern.edu] On Behalf > Of > Victoria > Baker > Sent: Friday, October 15, 2010 9:26 AM > To: Histo Net list server > Subject: [Histonet] negative controls > Hi > I have a hypothetical question to those who run IHC on Ventana > instruments. > Are you running your negatives with your patient/test cases or on a > separate > run? Also, if you are doing this and have to use a different > detection > kit > how do you work the QA/QC portion of this for CAP requirements. > Thanks > Vikki > _______________________________________________ > Histonet mailing list > [6]Histonet@lists.utsouth= western.edu > [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > [8]Histonet@lists.utsouth= western.edu > [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet > > References > > 1. 3D"mailto:LSebree@uwhealth.org" > 2. 3D"mailto:bakevictoria@gmail.com" > 3. 3D"mailto:HistoNet@lists.utsouthwestern.edu" > 4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" > 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" > 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" > 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BSullivan <@t> shorememorial.org Fri Oct 15 11:00:39 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Fri Oct 15 11:03:31 2010 Subject: [Histonet] negative controls In-Reply-To: <20101015084750.9e2d9aa830e8449a2412eb1e4f2f067e.1f487b8a54.wbe@email04.secureserver.net> Message-ID: We're talking NEGATIVE controls here folks and if you use internal tissue for your positive and negative controls...........they should all be processed and blocked in the same fashion as your actual patient material. We have scrutinized the negative control issue on the CAP checklist and have decided that if tissue warrants it, we cut an extra patient slide and it is run as the negative for that case. All is processed the same and stained identically with exception of the primary antibody. If there is not adequate material we use the Positive control and treat it negatively. This is reflected in our policy and procedure. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Sent by: To histonet-bounces@ "Rene J Buesa" lists.utsouthwest cc ern.edu Histo Net list server , Sebree Linda A 10/15/2010 11:47 AM Subject RE: [Histonet] negative controls So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: Rene J Buesa Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A , sgoebel@xbiotech.com Cc: Histo Net list server Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. Ren? J. --- On Fri, 10/15/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: RE: [Histonet] negative controls To: "Sebree Linda A" Cc: "Histo Net list server" Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician<= br> XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744 = (512)386-= 5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: "Sebree Linda A" <[1]LSebree@= uwhealth.org> Date: Fri, October 15, 2010 8:08 am To: "Victoria Baker" <[2]bakevict= oria@gmail.com>, "Histo Net list server" <[3]HistoNet@lists.uts= outhwestern.edu> We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [4]histonet= -bounces@lists.utsouthwestern.edu [[5]mailto:histon= et-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list [6]Histonet@lists.utsouth= western.edu [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouth= western.edu [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:LSebree@uwhealth.org" 2. 3D"mailto:bakevictoria@gmail.com" 3. 3D"mailto:HistoNet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Oct 15 11:07:59 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Oct 15 11:08:05 2010 Subject: [Histonet] negative controls In-Reply-To: <20101015084750.9e2d9aa830e8449a2412eb1e4f2f067e.1f487b8a54.wbe@email04.secureserver.net> References: <20101015084750.9e2d9aa830e8449a2412eb1e4f2f067e.1f487b8a54.wbe@email04.secureserver.net> Message-ID: <8C023B4AB999614BA4791BAEB26E273839A051@UWHC-MAIL01.uwhis.hosp.wisc.edu> To clarify even further: we cut 2 patient sections, put one on a slide with a section of positive control already on it. This slide gets stained with the antibody The other section goes on another slide and is run as a corresponding negative control using the same antibody protocol but substituting a negative control serum for the antibody, thus this is a "negative reagent control" slide. Elements within the patient slide that received antibody and are expected to be negative, serve as a "negative tissue control". Again, we run 1 negative control slide for EVERY 1 patient block in a run but only 1 negative control per any number of antibodies run on that same block, using the harshest protocol. Only autopsy cases differ in that we run 1 negative control per TISSUE TYPE. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: sgoebel@xbiotech.com [mailto:sgoebel@xbiotech.com] Sent: Friday, October 15, 2010 10:48 AM To: Rene J Buesa Cc: Histo Net list server; Sebree Linda A Subject: RE: [Histonet] negative controls So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: Rene J Buesa Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A , sgoebel@xbiotech.com Cc: Histo Net list server Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. Ren? J. --- On Fri, 10/15/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: RE: [Histonet] negative controls To: "Sebree Linda A" Cc: "Histo Net list server" Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician<= br> XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744 = (512)386-= 5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: "Sebree Linda A" <[1]LSebree@= uwhealth.org> Date: Fri, October 15, 2010 8:08 am To: "Victoria Baker" <[2]bakevict= oria@gmail.com>, "Histo Net list server" <[3]HistoNet@lists.uts= outhwestern.edu> We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [4]histonet= -bounces@lists.utsouthwestern.edu [[5]mailto:histon= et-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list [6]Histonet@lists.utsouth= western.edu [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouth= western.edu [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:LSebree@uwhealth.org" 2. 3D"mailto:bakevictoria@gmail.com" 3. 3D"mailto:HistoNet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Oct 15 11:13:11 2010 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Oct 15 11:13:14 2010 Subject: [Histonet] negative controls In-Reply-To: References: <20101015084750.9e2d9aa830e8449a2412eb1e4f2f067e.1f487b8a54.wbe@email04.secureserver.net> Message-ID: <8C023B4AB999614BA4791BAEB26E273839A052@UWHC-MAIL01.uwhis.hosp.wisc.edu> Beatrice, If there is not adequate tissue to cut a negative control, the pathologist has to make a "priority" decision so as to allow 1 slide to be treated as the negative control. If for some reason, there is only 1 slide available, i.e. no slide available to use as a negative control, we do not run the test. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: BSullivan@shorememorial.org [mailto:BSullivan@shorememorial.org] Sent: Friday, October 15, 2010 11:01 AM To: sgoebel@xbiotech.com Cc: Histo Net list server; histonet-bounces@lists.utsouthwestern.edu; Sebree Linda A; Rene J Buesa Subject: RE: [Histonet] negative controls We're talking NEGATIVE controls here folks and if you use internal tissue for your positive and negative controls...........they should all be processed and blocked in the same fashion as your actual patient material. We have scrutinized the negative control issue on the CAP checklist and have decided that if tissue warrants it, we cut an extra patient slide and it is run as the negative for that case. All is processed the same and stained identically with exception of the primary antibody. If there is not adequate material we use the Positive control and treat it negatively. This is reflected in our policy and procedure. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Sent by: To histonet-bounces@ "Rene J Buesa" lists.utsouthwest cc ern.edu Histo Net list server , Sebree Linda A 10/15/2010 11:47 AM Subject RE: [Histonet] negative controls So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: Rene J Buesa Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A , sgoebel@xbiotech.com Cc: Histo Net list server Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. Ren? J. --- On Fri, 10/15/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: RE: [Histonet] negative controls To: "Sebree Linda A" Cc: "Histo Net list server" Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician<= br> XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744 = (512)386-= 5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: "Sebree Linda A" <[1]LSebree@= uwhealth.org> Date: Fri, October 15, 2010 8:08 am To: "Victoria Baker" <[2]bakevict= oria@gmail.com>, "Histo Net list server" <[3]HistoNet@lists.uts= outhwestern.edu> We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [4]histonet= -bounces@lists.utsouthwestern.edu [[5]mailto:histon= et-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list [6]Histonet@lists.utsouth= western.edu [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouth= western.edu [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:LSebree@uwhealth.org" 2. 3D"mailto:bakevictoria@gmail.com" 3. 3D"mailto:HistoNet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Fri Oct 15 11:15:46 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Oct 15 11:15:51 2010 Subject: [Histonet] Update On New Job Openings Message-ID: Allied Search Partners has been retained for the following searches. 1. Please email a copy of updated resume to Alyssa@alliedsearchpartners.com for a full job description. 2. Please send availability for a phone screen with one of our recruiters. 3. Please indicate which position(s) you are interested in and which City/State We have the following positions available: 1. *Immuniohistochemistry Lead Tech (III) (2 positions available)* LOCATION: Port Chester, NY area (Long Island) SCHEDULE & DEPARTMENT: 9:30PM-6AM Monday-Friday (IHC Department) * * 1. *Immuniohistochemistry Tech II * LOCATION: Port Chester, NY area (Long Island) SCHEDULE & DEPARTMENT: 1pm-9:30pm Monday-Friday (IHC Department) 1. *Grossing Tech Lead (III)* LOCATION: Port Chester, NY area (Long Island) SCHEDULE AND DEPARTMENT: 8AM-4:30PM Tuesday-Saturday (Histology) 1. *Histotech I* LOCATION: Port Chester, NY area (Long Island) SCHEDULE & DEPARTMENT: 3PM-11:30PM Tuesday-Saturday (Histology) 1. *Histotechnician/Histotechnologist* LOCATION: Hicksville, NY area (Long Island) SCHEDULE & DEPARTMENT: 8AM-4:30PM Monday-Friday (Histology) 1. *Histotechnician/Histotechnologist* * * LOCATION: Baton Rouge, LA SCHEDULE & DEPARTMENT: 12PM-8:30AM Monday-Friday (Histology & IHC) 1. *Histotechnician/Histotechnologist* * * LOCATION: Lakeland, FL SCHEDULE & DEPARMENT: 3AM-11:30AM Monday-Friday (Histology & IHC) 1. *Cytoprep Technician (3 positions available)* LOCATION: Port Chester, NY area (Long Island) SCHEDULE & DEPARTMENT: 10PM-6:20 AM Monday-Friday (Cytology Department) 9PM-5:30AM Monday-Friday (Cytology Department) Day Shift Monday-Friday (Integrated Diagnostics Department) -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From Vickroy.Jim <@t> mhsil.com Fri Oct 15 11:37:22 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Oct 15 11:37:31 2010 Subject: [Histonet] Bone Marrow Trephines Message-ID: <24A4826E8EF0964D86BC5317306F58A554FE28E49F@mmc-mail.ad.mhsil.com> I sent out an email around two months ago regarding a problem with our bone marrow trephines. Our pathologist says that many of the trephines appear to be fragmented and although this has not prohibited determining a diagnosis it is still a problem. Currently we use AZF as a fixative and decal the trephines in Rapid Immuno Cal by BBC. We have investigated our times in each solution and have found that our hematopathologists often want us to rush the cases and the time in AZF is probably less than some other institutions. We use a minimum time of 3 hours and try to have most of the biopsies fix for around 6 -8 hours. Our standard of time in the decal solution is 1.5 hrs. (Variable depending on the amount of hard bone in the biopsy). I have also wondered whether the "fragmentation" may be a result of the sectioning technique, time the sections float on the waterbath, or temperature of the waterbath. We have not noticed this artifact in the routine tissues and on recutting sometimes the artifact is diminished but not completely gone. Would anybody be willing to share with me their protocols so that we can continue to investigate a cause of the 'fragmentation"? Any help would be appreciated. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Farnana <@t> nehealth.com Fri Oct 15 11:39:45 2010 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Fri Oct 15 11:39:51 2010 Subject: [Histonet] AFB stain Message-ID: <4CB84B91.26ED.00D9.1@nehealth.com> Have any of you know heard of AFB will picking up in any other organisms beside mycobacterium? Amy Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From Melissa.Kuhnla <@t> chsli.org Fri Oct 15 11:49:35 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri Oct 15 11:50:06 2010 Subject: [Histonet] negative controls In-Reply-To: References: Message-ID: Hi Vikki, I have 1 Ventana XT and 3 Ultras. I have certain antibodies designated to each ultra. This means not all slides from any case are guaranteed to be on the same machine. They obviously all have their own detection kit. My theory is that our detection kits are QCd prior to initial load onto an instrument. The patient block was treated the same prior to cutting. The slides receive solutions that are QCd...I think we are covered. What are your thoughts? Melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 10:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From Marilyn.A.Weiss <@t> kp.org Fri Oct 15 12:00:18 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Oct 15 12:00:36 2010 Subject: [Histonet] I Message-ID: I will be out of the office starting 10/14/2010 and will not return until 10/18/2010. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. From tjasper <@t> copc.net Fri Oct 15 12:32:28 2010 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Oct 15 12:32:34 2010 Subject: [Histonet] Locking up formalin References: Message-ID: <90354A475B420441B2A0396E5008D49692BFA4@copc-sbs.COPC.local> Hi Victoria, I've never heard of this, of course it could exist somewhere. I wonder what would prompt this. There are obvious safety issues to consider. Perhaps an unauthorized party, somewhere, got into some formalin and caused problems? Locked up or not, proper spill containment is mandated by OSHA (I believe) so, again perhaps an unauthorized personnel issue? I've worked in the Upper Midwest and Pacific Northwest and have not heard of this. I regularly attend the NSH and have not heard anything at the meetings either. Thomas Jasper Histology Supervisor Central Oregon Regional Path -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Spoon, Victoria Sent: Thursday, October 14, 2010 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Locking up formalin Is anyone aware of regulations stating that formalin has to be locked up- put in locked cabinets when not under direct supervision? Applying to either clinics where specimens are collected into formalin containers or in the pathology lab? Thank you Victoria Spoon Anatomic Pathology Manager Bassett Medical Center Cooperstown NY 13326 victoria.spoon@bassett.org Tel(607) 547-6357 Fax(607) 547-3203 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Debora.Probst <@t> crhs.net Fri Oct 15 12:53:30 2010 From: Debora.Probst <@t> crhs.net (Debora Probst) Date: Fri Oct 15 12:53:22 2010 Subject: [Histonet] IHC FILD Message-ID: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net> Can anyone tell me if once you have taken the IHC certification test and passed dose the administration consider that a specialty field and give you a pay increase? Or is it just for a persons own gratification to take it and pass? From BSullivan <@t> shorememorial.org Fri Oct 15 12:56:03 2010 From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org) Date: Fri Oct 15 12:58:52 2010 Subject: [Histonet] IHC FILD In-Reply-To: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net> Message-ID: I have never received any additional pay for any of my certifications. It is something I did for myself. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Debora Probst" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] IHC FILD 10/15/2010 01:53 PM Can anyone tell me if once you have taken the IHC certification test and passed dose the administration consider that a specialty field and give you a pay increase? Or is it just for a persons own gratification to take it and pass? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Fri Oct 15 13:35:18 2010 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Oct 15 13:35:27 2010 Subject: [Histonet] IHC FILD In-Reply-To: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net> References: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net> Message-ID: <4CB866A6.2B7F.00C9.1@geisinger.edu> In my case, it was for my own personal gratification. >>> "Debora Probst" 10/15/2010 1:53 PM >>> Can anyone tell me if once you have taken the IHC certification test and passed dose the administration consider that a specialty field and give you a pay increase? Or is it just for a persons own gratification to take it and pass? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From scorpionrider <@t> cox.net Fri Oct 15 13:46:59 2010 From: scorpionrider <@t> cox.net (Mark Turner) Date: Fri Oct 15 13:47:03 2010 Subject: [Histonet] IHC FILD In-Reply-To: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net> Message-ID: <20101015144659.DY0Z9.727743.imail@fed1rmwml29> Since it wasn't a requirement for my position, I took the exam for my own personal satisfaction. I highly recommend taking it if you have the chance and not worrying about whether you will receive extra money. Successfully passing the exam is a personal milestone and may not be recognized by your current employer, however it just might make a future one consider investing a little stronger in you because you have invested in yourself. Good luck! Mark Turner ---- Debora Probst wrote: > Can anyone tell me if once you have taken the IHC certification test and > passed dose the administration consider that a specialty field and give > you a pay increase? Or is it just for a persons own gratification to > take it and pass? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Fri Oct 15 13:49:39 2010 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Fri Oct 15 13:49:43 2010 Subject: [Histonet] High Paying Jobs in Laboratory Message-ID: <186875010.1287168625857.JavaMail.cfservice@sl4app2> Dear Beatrice, I hope you are doing well. I am a recruiter at a highly successful and well respected healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to job seekers and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working with a private non-profit acute care hospital in Vermont. My client is looking to hire a full-time Medical Technologist for their 3rd shift. The shift is composed of three 12.5 hour shifts. The ideal candidate must be a certified Medical Technologist by the ASCP or equivalent and have 2+ years experience as a generalist. This is a fantastic opportunity at an outstanding hospital with a great reputation in this community. My clients offers some of the best benefits and compensation package around, including childcare reimbursement, commuter assistance, a retirement plan with a generous match, relocation assistance and tuition reimbursement. Additionally, the night shift comes with a generous shift differential. If you are interested in learning more about this position, please call or email me. Below is a list of some of the other great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Laboratory Opportunities (I just updated this list TODAY!!!): Medical Technologist/ Clinical Lab Scientist: * NY - Rochester - Medical Technologist (3rd shift) * NY - North of Albany - Medical Technologist (2nd shift) * NY - Lake Placid - Medical Technologist (2nd or 3rd shift) * NY - West of Albany - Medical Technologist (All Shifts) * NY - Syracuse - Medical Technologist (2nd or 3rd shift) * NY - NYC - Lead Medical Technologist (2nd shift) * NY - Long Island - Medical Technologist (1st shift) * PA - Lehigh Valley - Medical Technologist (2nd shift) * CT - Medical Technologist (3rd shift) * CT - MLT (1st shift) * VT - Medical Technologist (3rd shift with 12 hours shifts) * GA - Hematology Medical Technologist (1st shift) * FL - Treasure Coast - Medical Technologist (3rd shift) * OR - Coastal - Medical Technologist (3rd shift) * OR - Medical Technologist (all shifts) * WA - Medical Technologist * AK - Medical Technologist * NM - Medical Technologist (1st shift) * CA - Los Angeles - Medical Technologist (all shifts) * CA - Central Valley (rural) - Medical Technologist (3rd shift) * CA - Sacramento - Laboratory Specialist Lab Supervisor/Management Opportunities: * MS - 2nd/3rd shift Laboratory Supervisor * CT - Hematology Supervisor * TX - Director of Laboratory Operations for Hospital System * KY - Laboratory Supervisor * CA - Los Angeles - Laboratory Supervisor * TX - San Antonio - Laboratory Supervisor * GA - HLA Supervisor * PA - Director of Laboratory Operations * NJ - General Manager of Anatomic Pathology * WA - Quality Control Supervisor Blood Bank (Bench and Management) * WA - Transfusion Services Supervisor * WA - Blood Bank Specialist * WA - Blood Bank Educator * IA - Blood Bank Technologist (2nd shift) * NY - NYC - Immunohematologist (4pm12am shift) * CA - San Fran - Specialist in Blood Bank * MN - Component Laboratory Manager/ Blood Bank Supervisor * NY - Syracuse - Blood Bank Specialist * VA - Blood Bank Supervisor Microbiology (Bench and Management) * OR - Microbiology Technologist * NY - Microbiology Lead Technologist * MS - Microbiology Supervisor * OR - Manager of Microbiology and Molecular Diagnostics * CA - Los Angeles - Mycobacteriology Technologist * NY - Microbiology Manager * OH - Cinci - Microbiology Technologist (1st shift) Cytotech and Histotech (Bench and Management) * NY - NYC - Cytology Supervisor * NY - Syracuse - Histotech * NY - NYC - Histotech (3rd shift) * NY - Long Island - Histology Manager with Derm experience * MI - Histology Manager with Derm experience * OK - Histology Supervisor * GA - Histology Supervisor * NV - Histotech (3rd shift) * TN - Histotech (1st shift) * MA - Cape Cod - Histotech * TX - Western - Histology Supervisor Cytogenetics and Other Specialities: * NY - Toxicology Technologist * NY - Bone Marrow Technologist * OR - Toxicology Technologist * MA - Cytogenetics Technologist * NJ - Northern - Cytogenetics Technologist * MD - Cytogenetics Technologist * NJ - Northern - FISH Supervisor Pathologist Assistant: * NV - Pathologist Assistant Pathologist Jobs: * AZ - Dermatopathologist * NJ - Medical Director (licensed in anatomic and clinical pathology) * NJ - Hematopathologist * NJ - Cytopathologist * NY - Chief Pathologist If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com This email was sent to histonet@lists.utsouthwestern.edu, by Alisha Dynan To remove your email address permanently from future mailings, please go to the following URL: http://cls6.bullhornstaffing.com/MailerUnsubscribe.cfm?privateLabelID=2533&email=histonet%40lists%2Eutsouthwestern%2Eedu&updKey=L%28U%27%2C%20%21A%3BIJKBE%29%228%22LC8%5FU%3A7GKM%3FTB%22R%2E%29M%2A2%3AN%2ALHY%5FC%5C%3FSC24%3A%23K%5B9JVH%20%0A From Kim.Donadio <@t> bhcpns.org Fri Oct 15 14:23:32 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Fri Oct 15 14:23:37 2010 Subject: [Histonet] Histotech needed in awesome Pensacola Florida In-Reply-To: <186875010.1287168625857.JavaMail.cfservice@sl4app2> Message-ID: Just a reminder for the weekend for all the Histotechs who are seeking a great position in a great place to live. I have a position coming available Very soon. I'm not a recruiter, just a supervisor who works the bench with you daily looking for someone smart and fun :-) Have a great weekend everyone! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From STACEY.LANGENBERG <@t> UCDENVER.EDU Fri Oct 15 14:25:33 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Fri Oct 15 14:25:43 2010 Subject: [Histonet] IHC FILD In-Reply-To: <20101015144659.DY0Z9.727743.imail@fed1rmwml29> References: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net><20101015144659.DY0Z9.727743.imail@fed1rmwml29> Message-ID: <1225598847.2287268.1287170737616.JavaMail.rim@bda2340.bisx.prod.on.blackberry> Well said Mark! Sent via BlackBerry from T-Mobile -----Original Message----- From: Mark Turner Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Fri, 15 Oct 2010 12:46:59 To: Debora Probst; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC FILD Since it wasn't a requirement for my position, I took the exam for my own personal satisfaction. I highly recommend taking it if you have the chance and not worrying about whether you will receive extra money. Successfully passing the exam is a personal milestone and may not be recognized by your current employer, however it just might make a future one consider investing a little stronger in you because you have invested in yourself. Good luck! Mark Turner ---- Debora Probst wrote: > Can anyone tell me if once you have taken the IHC certification test and > passed dose the administration consider that a specialty field and give > you a pay increase? Or is it just for a persons own gratification to > take it and pass? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Oct 15 16:11:47 2010 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Oct 15 16:11:52 2010 Subject: [Histonet] negative controls References: <20101015084750.9e2d9aa830e8449a2412eb1e4f2f067e.1f487b8a54.wbe@email04.secureserver.net> <8C023B4AB999614BA4791BAEB26E273839A051@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: Linda, My system of negative controls is identical to yours. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Sebree Linda A" To: ; "Rene J Buesa" Cc: "Histo Net list server" Sent: Friday, October 15, 2010 11:07 AM Subject: RE: [Histonet] negative controls To clarify even further: we cut 2 patient sections, put one on a slide with a section of positive control already on it. This slide gets stained with the antibody The other section goes on another slide and is run as a corresponding negative control using the same antibody protocol but substituting a negative control serum for the antibody, thus this is a "negative reagent control" slide. Elements within the patient slide that received antibody and are expected to be negative, serve as a "negative tissue control". Again, we run 1 negative control slide for EVERY 1 patient block in a run but only 1 negative control per any number of antibodies run on that same block, using the harshest protocol. Only autopsy cases differ in that we run 1 negative control per TISSUE TYPE. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: sgoebel@xbiotech.com [mailto:sgoebel@xbiotech.com] Sent: Friday, October 15, 2010 10:48 AM To: Rene J Buesa Cc: Histo Net list server; Sebree Linda A Subject: RE: [Histonet] negative controls So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: Rene J Buesa Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A , sgoebel@xbiotech.com Cc: Histo Net list server Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. Ren? J. --- On Fri, 10/15/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: RE: [Histonet] negative controls To: "Sebree Linda A" Cc: "Histo Net list server" Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician<= br> XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744 = (512)386-= 5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: "Sebree Linda A" <[1]LSebree@= uwhealth.org> Date: Fri, October 15, 2010 8:08 am To: "Victoria Baker" <[2]bakevict= oria@gmail.com>, "Histo Net list server" <[3]HistoNet@lists.uts= outhwestern.edu> We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [4]histonet= -bounces@lists.utsouthwestern.edu [[5]mailto:histon= et-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list [6]Histonet@lists.utsouth= western.edu [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [8]Histonet@lists.utsouth= western.edu [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:LSebree@uwhealth.org" 2. 3D"mailto:bakevictoria@gmail.com" 3. 3D"mailto:HistoNet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:Histonet@lists.utsouthwestern.edu" 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 8. 3D"mailto:Histonet@lists.utsouthwestern.edu" 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Oct 15 17:22:41 2010 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Oct 15 17:22:56 2010 Subject: [Histonet] IHC FILD In-Reply-To: References: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net> Message-ID: I did not receive a pay increas. Personal gratification and a sense of accomplishment. It was not a walk in the park by any stretch of the imagination. I learned a great deal from taking it. - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BSullivan@shorememorial.org Sent: Friday, October 15, 2010 12:56 PM To: Debora Probst Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC FILD I have never received any additional pay for any of my certifications. It is something I did for myself. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "Debora Probst" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] IHC FILD 10/15/2010 01:53 PM Can anyone tell me if once you have taken the IHC certification test and passed dose the administration consider that a specialty field and give you a pay increase? Or is it just for a persons own gratification to take it and pass? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abeharry798 <@t> gmail.com Fri Oct 15 19:58:26 2010 From: abeharry798 <@t> gmail.com (Andrea) Date: Fri Oct 15 19:52:46 2010 Subject: [Histonet] IHC FILD In-Reply-To: <1225598847.2287268.1287170737616.JavaMail.rim@bda2340.bisx.prod.on.blackberry> References: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net><20101015144659.DY0Z9.727743.imail@fed1rmwml29> <1225598847.2287268.1287170737616.JavaMail.rim@bda2340.bisx.prod.on.blackberry> Message-ID: Hi, how do you go about taking this test. are there prerequisite courses to take? On 2010-10-15, at 3:25 PM, "Langenberg, Stacey" wrote: > Well said Mark! > Sent via BlackBerry from T-Mobile > > -----Original Message----- > From: Mark Turner > Sender: "histonet-bounces@lists.utsouthwestern.edu" > > Date: Fri, 15 Oct 2010 12:46:59 > To: Debora Probst; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC FILD > > Since it wasn't a requirement for my position, I took the exam for my own personal satisfaction. I highly recommend taking it if you have the chance and not worrying about whether you will receive extra money. Successfully passing the exam is a personal milestone and may not be recognized by your current employer, however it just might make a future one consider investing a little stronger in you because you have invested in yourself. > > Good luck! > > Mark Turner > > > ---- Debora Probst wrote: >> Can anyone tell me if once you have taken the IHC certification test and >> passed dose the administration consider that a specialty field and give >> you a pay increase? Or is it just for a persons own gratification to >> take it and pass? >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Oct 15 23:55:46 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Oct 15 23:55:55 2010 Subject: [Histonet] IHC FILD In-Reply-To: Message-ID: http://www.ascp.org/FunctionalNavigation/certification/QualificationinImmunohistochemistryQIHC.aspx Here is the information from the ASCP website. Andrea Sent by: histonet-bounces@lists.utsouthwestern.edu 10/15/2010 05:56 PM To "Langenberg, Stacey" cc Debora Probst , "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Subject Re: [Histonet] IHC FILD Hi, how do you go about taking this test. are there prerequisite courses to take? On 2010-10-15, at 3:25 PM, "Langenberg, Stacey" wrote: > Well said Mark! > Sent via BlackBerry from T-Mobile > > -----Original Message----- > From: Mark Turner > Sender: "histonet-bounces@lists.utsouthwestern.edu" > > Date: Fri, 15 Oct 2010 12:46:59 > To: Debora Probst; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC FILD > > Since it wasn't a requirement for my position, I took the exam for my own personal satisfaction. I highly recommend taking it if you have the chance and not worrying about whether you will receive extra money. Successfully passing the exam is a personal milestone and may not be recognized by your current employer, however it just might make a future one consider investing a little stronger in you because you have invested in yourself. > > Good luck! > > Mark Turner > > > ---- Debora Probst wrote: >> Can anyone tell me if once you have taken the IHC certification test and >> passed dose the administration consider that a specialty field and give >> you a pay increase? Or is it just for a persons own gratification to >> take it and pass? >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Sat Oct 16 07:16:57 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Oct 16 07:17:05 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16403A6810936@CHEXCMS10.one.ads.che.org> Message-ID: <9B23F2471BAD4C7D8B76BFCDB1225445@hopperPC> Joyce, THANK YOU for the link! :o) Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update? This is really going to help the "widget" count in my lab! My paths frequently want to test more than one block on their tumor cases and now I can charge for them! :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Friday, October 15, 2010 10:45 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 http://www.flpath.org/rli2.asp Here is a link that will help.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" wrote: > > CMS/NCCI Update Dated October 1, 2009 > > 8. The unit of service for special stains (CPT codes 88312-88313) and > immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If > it is medically reasonable and necessary to perform the same stain on > more than one specimen or more than one block of tissue from the same > specimen, additional units of service may be reported for the > additional specimen(s) or block(s). Physicians should not report more > than one unit of service for a stain performed on a single tissue > block. For example it is common practice to cut multiple levels from a > tissue block and stain each level with the same stain. The multiple > levels from the same block of tissue stained with the same stain > should not be reported as additional units of service. Only one unit > of service should be reported for the stain on multiple levels from > the single tissue block. Additionally, controls performed with special > stains should not be reported as separate units of service for the > stain. > > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Thursday, October 14, 2010 11:31 > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Can you site your source, please. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Thursday, October 14, 2010 10:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > > > > The change is that you can bill per block now and not per specimen. > This is for immunos and special stains. It does make a huge difference! > > Best, > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris > Evanish > Sent: Thursday, October 14, 2010 11:10 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Has anyone heard of a cpt coding change that allows us to bill 88342 > per slide run instead of per antibody? One of our Pathologist was at a > conference and was told that we could do that. It makes a big > difference with running cytokeratins on multiple blocks and levels of > sentinel nodes. > > Thanks, > Chris Evanish > Montgomery Hospital > Norristown PA > > Chris D. Evanish > Histology Supervisor > Montgomery Hospital > 610-270-2379 > > Please consider the environment before printing this email " to your > outgoing mail. > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 From ccrowder <@t> vetmed.lsu.edu Sat Oct 16 09:48:53 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Sat Oct 16 09:50:06 2010 Subject: [Histonet] AFB Message-ID: Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast bacteria. The Fite's stain is usually considered specific for mycobacteria. Cheryl From pruegg <@t> ihctech.net Sat Oct 16 10:19:02 2010 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat Oct 16 10:19:47 2010 Subject: [Histonet] IHC FILD Message-ID: <77767910$512c0944$37ef194b$@com> it depends on who you are working with, it would be a requirement to work for me since i specialize in IHC, but other employers may not value it as much, in any case as Mark said, it is investing in yourself. ---------------------------------------- From: "Langenberg, Stacey" Sent: Friday, October 15, 2010 1:29 PM To: "Mark Turner" , "histonet-bounces@lists.utsouthwestern.edu" , "Debora Probst" , "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC FILD Well said Mark! Sent via BlackBerry from T-Mobile -----Original Message----- From: Mark Turner Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Fri, 15 Oct 2010 12:46:59 To: Debora Probst; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC FILD Since it wasn't a requirement for my position, I took the exam for my own personal satisfaction. I highly recommend taking it if you have the chance and not worrying about whether you will receive extra money. Successfully passing the exam is a personal milestone and may not be recognized by your current employer, however it just might make a future one consider investing a little stronger in you because you have invested in yourself. Good luck! Mark Turner ---- Debora Probst wrote: > Can anyone tell me if once you have taken the IHC certification test and > passed dose the administration consider that a specialty field and give > you a pay increase? Or is it just for a persons own gratification to > take it and pass? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Sat Oct 16 10:32:33 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Oct 16 10:32:38 2010 Subject: [Histonet] negative controls In-Reply-To: Message-ID: I run both positive and negative controls. Wherever possible, I put the positive control on the same slide as the test patient (this makes it easier for the pathologist and it also saves us the cost of on slide being tested). I always run a negative patient control on the exact same run. If I have more slides than will fit on one run, I will hold the specific case back, as I won't separate them. Matters not if there is more than one machine, the case will stay together. As far as negatives, I will run an extra negative if my run has both poly and mono antibodies. So, there are many times, I have two negatives running per case. I run only one detection system, so I don't have that variable to deal with. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Friday, October 15, 2010 12:50 PM To: Victoria Baker; Histo Net list server Subject: RE: [Histonet] negative controls Hi Vikki, I have 1 Ventana XT and 3 Ultras. I have certain antibodies designated to each ultra. This means not all slides from any case are guaranteed to be on the same machine. They obviously all have their own detection kit. My theory is that our detection kits are QCd prior to initial load onto an instrument. The patient block was treated the same prior to cutting. The slides receive solutions that are QCd...I think we are covered. What are your thoughts? Melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 10:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 From aazath <@t> hotmail.com Sat Oct 16 14:35:28 2010 From: aazath <@t> hotmail.com (Aazath Raj) Date: Sat Oct 16 14:35:33 2010 Subject: [Histonet] Microsporidia In-Reply-To: References: Message-ID: Dear Friends, Can anyone tell me how to demonstrate Micrsporidia in Deudenal biopsy.For me the modified Brown and bren gram is not working.if u have any other method pls let me know. with regrads, Aazath From JWeems <@t> sjha.org Sun Oct 17 17:46:54 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sun Oct 17 17:47:01 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <9B23F2471BAD4C7D8B76BFCDB1225445@hopperPC> References: <92AD9B20A6C38C4587A9FEBE3A30E16403A6810936@CHEXCMS10.one.ads.che.org> <9B23F2471BAD4C7D8B76BFCDB1225445@hopperPC> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403A6810A2F@CHEXCMS10.one.ads.che.org> I posted it when I found out about it last October. Sorry to everyone that didn't see it! j -----Original Message----- From: histotech@imagesbyhopper.com [mailto:histotech@imagesbyhopper.com] Sent: Saturday, October 16, 2010 08:17 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Joyce, THANK YOU for the link! :o) Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update? This is really going to help the "widget" count in my lab! My paths frequently want to test more than one block on their tumor cases and now I can charge for them! :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Friday, October 15, 2010 10:45 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 http://www.flpath.org/rli2.asp Here is a link that will help.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" wrote: > > CMS/NCCI Update Dated October 1, 2009 > > 8. The unit of service for special stains (CPT codes 88312-88313) and > immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If > it is medically reasonable and necessary to perform the same stain on > more than one specimen or more than one block of tissue from the same > specimen, additional units of service may be reported for the > additional specimen(s) or block(s). Physicians should not report more > than one unit of service for a stain performed on a single tissue > block. For example it is common practice to cut multiple levels from a > tissue block and stain each level with the same stain. The multiple > levels from the same block of tissue stained with the same stain > should not be reported as additional units of service. Only one unit > of service should be reported for the stain on multiple levels from > the single tissue block. Additionally, controls performed with special > stains should not be reported as separate units of service for the > stain. > > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Thursday, October 14, 2010 11:31 > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Can you site your source, please. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Thursday, October 14, 2010 10:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > > > > The change is that you can bill per block now and not per specimen. > This is for immunos and special stains. It does make a huge difference! > > Best, > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris > Evanish > Sent: Thursday, October 14, 2010 11:10 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Has anyone heard of a cpt coding change that allows us to bill 88342 > per slide run instead of per antibody? One of our Pathologist was at a > conference and was told that we could do that. It makes a big > difference with running cytokeratins on multiple blocks and levels of > sentinel nodes. > > Thanks, > Chris Evanish > Montgomery Hospital > Norristown PA > > Chris D. Evanish > Histology Supervisor > Montgomery Hospital > 610-270-2379 > > Please consider the environment before printing this email " to your > outgoing mail. > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From JWeems <@t> sjha.org Sun Oct 17 17:47:41 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sun Oct 17 17:47:46 2010 Subject: [Histonet] AFB In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403A6810A30@CHEXCMS10.one.ads.che.org> I thought Fite's was more specific for leprosy.. whatever that bug is called now. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Saturday, October 16, 2010 10:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast bacteria. The Fite's stain is usually considered specific for mycobacteria. Cheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From histotech <@t> imagesbyhopper.com Sun Oct 17 18:15:29 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun Oct 17 18:15:44 2010 Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16403A6810A2F@CHEXCMS10.one.ads.che.org> Message-ID: Joyce, Certainly not *your* fault that *I* missed it! THANK YOU for reposting the link. :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Sunday, October 17, 2010 6:47 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 I posted it when I found out about it last October. Sorry to everyone that didn't see it! j -----Original Message----- From: histotech@imagesbyhopper.com [mailto:histotech@imagesbyhopper.com] Sent: Saturday, October 16, 2010 08:17 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Joyce, THANK YOU for the link! :o) Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update? This is really going to help the "widget" count in my lab! My paths frequently want to test more than one block on their tumor cases and now I can charge for them! :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Friday, October 15, 2010 10:45 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 http://www.flpath.org/rli2.asp Here is a link that will help.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" wrote: > > CMS/NCCI Update Dated October 1, 2009 > > 8. The unit of service for special stains (CPT codes 88312-88313) and > immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If > it is medically reasonable and necessary to perform the same stain on > more than one specimen or more than one block of tissue from the same > specimen, additional units of service may be reported for the > additional specimen(s) or block(s). Physicians should not report more > than one unit of service for a stain performed on a single tissue > block. For example it is common practice to cut multiple levels from a > tissue block and stain each level with the same stain. The multiple > levels from the same block of tissue stained with the same stain > should not be reported as additional units of service. Only one unit > of service should be reported for the stain on multiple levels from > the single tissue block. Additionally, controls performed with special > stains should not be reported as separate units of service for the > stain. > > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Thursday, October 14, 2010 11:31 > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Can you site your source, please. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Thursday, October 14, 2010 10:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > > > > The change is that you can bill per block now and not per specimen. > This is for immunos and special stains. It does make a huge difference! > > Best, > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris > Evanish > Sent: Thursday, October 14, 2010 11:10 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Has anyone heard of a cpt coding change that allows us to bill 88342 > per slide run instead of per antibody? One of our Pathologist was at a > conference and was told that we could do that. It makes a big > difference with running cytokeratins on multiple blocks and levels of > sentinel nodes. > > Thanks, > Chris Evanish > Montgomery Hospital > Norristown PA > > Chris D. Evanish > Histology Supervisor > Montgomery Hospital > 610-270-2379 > > Please consider the environment before printing this email " to your > outgoing mail. > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). It may contain information that is privileged and > confidential. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you are not the intended recipient, > please delete this message, and reply to the sender regarding the > error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/17/10 18:33:00 From lpwenk <@t> sbcglobal.net Sun Oct 17 21:02:23 2010 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Oct 17 21:02:27 2010 Subject: [Histonet] AFB In-Reply-To: References: Message-ID: <474B6E602A164B3C90484AFE22963783@HP2010> Hi - Can't find the original question - must have deleted it, so I'll answer through Cheryl's, if that's OK with her. If I remember, the question was something like - are there other microorganisms besides TB that stain with Kinyoun (or something like that). First, any mycobacterium (acid fast bacteria AFB) will stain with Ziehl-Neelsen, Kinyoun, Auramine-Rhodamine or Fites (hence anything staining with these stains is called AFB positive, meaning staining with these AF stains) . So that means Mycobacterium tuberculosis (TB), Mycobacterium avium intracellular (found in birds and I've seen it in spleen and bone marrow of immune suppressed patients), Mycobacterium paratuberculosis (found in intestine of infected cows and goats, causing something similar to Crone's disease), and a lot of other Mycobacterium are AFB positive staining with these procedures. Mycobacterium leprae (leprosy) is a very thin walled mycobacterium, so needs the Fites stain with the peanut oil (or any other oil, such as mineral oil) to coat the leprosy microorganism, so it can withstand decolorization with aqueous alcohol. But Fites can also be used to demonstrate any of the Mycobacterium. These AFB stains will also demonstrate the sulfur clubs of actinomyces, and the filamentous strands of nocardia are also weakly AFB positive. (Sort of bacteria with some fungus characteristics.) Cryptosporidium found in the intestine (causing severe diarrhea) can also stain AFB positive. And there are bacterium (mycobacterium or others) in tap water that are AFB positive, which can adhere to your slides from the flotation bath water, or from the tap water. Usually non-pathogenic AFB, but I don't know their names. And I'm sure there are other less common bacteria that I've forgotten or never known about that will stain AFB positive, which are not in the Mycobacterium genus. And let's remember that hair follicles, sperm (heads with lipids), lipofucsin (wear and tear pigment of lipids) also stain AFB positive. And also Russell bodies (inclusions of antibodies in plasma cells) will stain AFB positive. And keratin which is dense can simply require longer differentiation, or else it will retain the red color of the dye. So, yes, Amy, there are other things that stain positive with Kinyoun that are not TB/mycobacterium. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Cheryl Crowder" Sent: Saturday, October 16, 2010 10:48 AM To: Subject: [Histonet] AFB > Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast > bacteria. The Fite's stain is usually considered specific for > mycobacteria. > Cheryl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Oct 18 04:51:36 2010 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon Oct 18 04:51:44 2010 Subject: [Histonet] autofluorescence Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9DF@EXC-MBX3.cfs.le.ac.uk> Any information on current methods to counter autoflourescence in formalin fixed paraffin processed tissues would be much appreciated. Many thanks Richard Edwards Leicester University U.K................ From stephanie.d.rivera <@t> gsk.com Mon Oct 18 08:13:07 2010 From: stephanie.d.rivera <@t> gsk.com (Stephanie Rivera) Date: Mon Oct 18 08:13:20 2010 Subject: [Histonet] RE: IHC FILD In-Reply-To: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net> References: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A785@crhs_exch.nterprise.crhs.net> Message-ID: <14A77A757E86BC499A34E86B47649B64054FC6BA7D@019D-NAMSG-05.019D.MGD.MSFT.NET> My company does not recognize the certification. It was just for my personal development. Some hospitals do recognize it as a specialty but I don't know if you get an increase. Check with your manager. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debora Probst Sent: Friday, October 15, 2010 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC FILD Can anyone tell me if once you have taken the IHC certification test and passed dose the administration consider that a specialty field and give you a pay increase? Or is it just for a persons own gratification to take it and pass? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Debora.Probst <@t> crhs.net Mon Oct 18 09:51:08 2010 From: Debora.Probst <@t> crhs.net (Debora Probst) Date: Mon Oct 18 09:50:58 2010 Subject: [Histonet] (no subject) Message-ID: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A93F@crhs_exch.nterprise.crhs.net> I would just like to thank everyone for their response back on IHC certification. What a great resource for our field (Histology). Again thank you everyone. Debora Probst Columbus Regional, Columbus Ga. From sgoebel <@t> xbiotech.com Mon Oct 18 09:53:00 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Oct 18 09:53:05 2010 Subject: [Histonet] Eosin Message-ID: <20101018075300.9e2d9aa830e8449a2412eb1e4f2f067e.1df96a717f.wbe@email04.secureserver.net> Once upon a time I heard that eosin fluoresces is this true? color does it show up? Could this be used as a sort of back gr stain so you can tell exactly how many cells are in the field? Sarah Goebel, B.A., HT (ASCP) Histotechnician 8201 East Riversid Austin, Texas (512)386-2907 From b-frederick <@t> northwestern.edu Mon Oct 18 10:04:23 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Oct 18 10:04:48 2010 Subject: [Histonet] Block release Message-ID: <0AD641BB647144BCBC1AE4CD68C18B22@lurie.northwestern.edu> Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From hfedor <@t> jhmi.edu Mon Oct 18 10:12:08 2010 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Oct 18 10:12:27 2010 Subject: [Histonet] Block release In-Reply-To: <0AD641BB647144BCBC1AE4CD68C18B22@lurie.northwestern.edu> References: <0AD641BB647144BCBC1AE4CD68C18B22@lurie.northwestern.edu> Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D31781F6FE65@JHEMTEXVS3.win.ad.jhu.edu> Hello, Our institution does release blocks, the requirement is that a patient consent is signed and we have an MTA (Material Transfer Agreement) For the study and appropriate IRB's in place for the study. It is a lot to set up, but after this is done for one study any following studies are easier to handle. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Mon Oct 18 10:22:33 2010 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Oct 18 10:22:44 2010 Subject: [Histonet] Block release In-Reply-To: <0AD641BB647144BCBC1AE4CD68C18B22@lurie.northwestern.edu> References: <0AD641BB647144BCBC1AE4CD68C18B22@lurie.northwestern.edu> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1395E57654@NADCWPMSGCMS03.hca.corpad.net> Good Morning to All, We, as often as possible, try not to release blocks for studies or legal cases. Most studies will let you submit 10-20 unstained recut slides on Plus slides. On legal cases, we always give recuts if there is enough tissue. When slides will not suffice, we will send the block and keep a record of the block being sent. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Oct 18 10:51:43 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 18 10:51:51 2010 Subject: [Histonet] Eosin In-Reply-To: <20101018075300.9e2d9aa830e8449a2412eb1e4f2f067e.1df96a717f.wbe@email04.secureserver.net> Message-ID: <9500.31952.qm@web65703.mail.ac4.yahoo.com> Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if you observe a solution with transmitted light. Ren? J. --- On Mon, 10/18/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: [Histonet] Eosin To: histonet@lists.utsouthwestern.edu Date: Monday, October 18, 2010, 10:53 AM ???Once? upon a time I heard that eosin fluoresces is this true? = ; What ???color? does it show up?? Could this be used as a sort of back gr= ound ???stain so you can tell exactly how many cells are in the field? ???Sarah Goebel, B.A., HT (ASCP) ???Histotechnician ???= = XBiotech USA Inc. ???8201 East Riversid= e Dr. Bldg 4 Suite 100 ???Austin, Texas=???78744 ???(512)386-2907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Mon Oct 18 10:58:44 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Mon Oct 18 10:58:52 2010 Subject: [Histonet] clinical trial blocks Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56FE@MAIL1.pph.local> The fundamental philosophy that we have is that we do not want to stand in the way of the patient receiving any kind of treatment. That being said, we also have the policy that whether or not we "own" the material (patients are actually suppose to sign an admission consent that says they give up any ownership rights), we are clearly the legal custodians of the material, and as such are responsible for insuring that no irreparable distruction occurs. So, we prefer to not send out blocks unless the clilnical trial says there are no alternatives. We contact them and ask if we can send recuts from which they can obtain the information they desire. We also review what we have. If we have only one section, we cut a recut for the file before sending out any blocks. If we have only one block, with not much in it, we again discuss the issue with the trial coordinator. We do insist on return of the block, and assuming that there is an outside review of the material, we insist on a copy of the report. We also have the patient sign a release informing them that they need to understand that this process may exhaust any tissue that would be useful for some other new treatment which may come up down the line. Since we have to pull this material for review and usually make recuts, we bill the clinical trial. Years back, they never had any budget allowances for this, and it was always a hassle. More recently, we get a lot less BS. They know they have to pay, and they do. Unfortunately, nothing is free any more. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From JABiedermann <@t> uams.edu Mon Oct 18 14:04:58 2010 From: JABiedermann <@t> uams.edu (Biedermann, JoAnn) Date: Mon Oct 18 14:03:58 2010 Subject: [Histonet] RE: Autofluorescence In-Reply-To: References: Message-ID: <833890C7E1BE584B926A2584E6B37ADC7D129233@MAIL2.ad.uams.edu> Richard, we had trouble with autofluorescence in brain tissue of the elderly. We have found that 1% Sudan Black B in 70% EtOH for 2 minutes before the secondary antibody works very well. Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173 Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 JABiedermann@uams.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, October 18, 2010 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 83, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Re: Histonet Digest, Vol 83, Issue 20 (Weems, Joyce) 2. RE: AFB (Weems, Joyce) 3. RE: Re: Histonet Digest, Vol 83, Issue 20 (histotech@imagesbyhopper.com) 4. Re: AFB (Lee & Peggy Wenk) 5. autofluorescence (Edwards, Richard E.) 6. RE: IHC FILD (Stephanie Rivera) 7. (no subject) (Debora Probst) 8. Eosin (sgoebel@xbiotech.com) 9. Block release (Bernice Frederick) 10. RE: Block release (Helen Fedor) 11. RE: Block release (Wanda.Smith@HCAhealthcare.com) 12. Re: Eosin (Rene J Buesa) 13. clinical trial blocks (Tench, Bill) ---------------------------------------------------------------------- Message: 1 Date: Sun, 17 Oct 2010 18:46:54 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 To: "histotech@imagesbyhopper.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403A6810A2F@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I posted it when I found out about it last October. Sorry to everyone that didn't see it! j -----Original Message----- From: histotech@imagesbyhopper.com [mailto:histotech@imagesbyhopper.com] Sent: Saturday, October 16, 2010 08:17 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Joyce, THANK YOU for the link! :o) Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update? This is really going to help the "widget" count in my lab! My paths frequently want to test more than one block on their tumor cases and now I can charge for them! :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Friday, October 15, 2010 10:45 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 http://www.flpath.org/rli2.asp Here is a link that will help.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" wrote: > > CMS/NCCI Update Dated October 1, 2009 > > 8. The unit of service for special stains (CPT codes 88312-88313) and > immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If > it is medically reasonable and necessary to perform the same stain on > more than one specimen or more than one block of tissue from the same > specimen, additional units of service may be reported for the > additional specimen(s) or block(s). Physicians should not report more > than one unit of service for a stain performed on a single tissue > block. For example it is common practice to cut multiple levels from a > tissue block and stain each level with the same stain. The multiple > levels from the same block of tissue stained with the same stain > should not be reported as additional units of service. Only one unit > of service should be reported for the stain on multiple levels from > the single tissue block. Additionally, controls performed with special > stains should not be reported as separate units of service for the > stain. > > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Thursday, October 14, 2010 11:31 > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Can you site your source, please. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Thursday, October 14, 2010 10:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > > > > The change is that you can bill per block now and not per specimen. > This is for immunos and special stains. It does make a huge difference! > > Best, > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris > Evanish > Sent: Thursday, October 14, 2010 11:10 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Has anyone heard of a cpt coding change that allows us to bill 88342 > per slide run instead of per antibody? One of our Pathologist was at a > conference and was told that we could do that. It makes a big > difference with running cytokeratins on multiple blocks and levels of > sentinel nodes. > > Thanks, > Chris Evanish > Montgomery Hospital > Norristown PA > > Chris D. Evanish > Histology Supervisor > Montgomery Hospital > 610-270-2379 > > Please consider the environment before printing this email " to your > outgoing mail. > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 2 Date: Sun, 17 Oct 2010 18:47:41 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] AFB To: Cheryl Crowder , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403A6810A30@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I thought Fite's was more specific for leprosy.. whatever that bug is called now. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Saturday, October 16, 2010 10:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast bacteria. The Fite's stain is usually considered specific for mycobacteria. Cheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Sun, 17 Oct 2010 19:15:29 -0400 From: Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 To: "'Weems, Joyce'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Joyce, Certainly not *your* fault that *I* missed it! THANK YOU for reposting the link. :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Sunday, October 17, 2010 6:47 PM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 I posted it when I found out about it last October. Sorry to everyone that didn't see it! j -----Original Message----- From: histotech@imagesbyhopper.com [mailto:histotech@imagesbyhopper.com] Sent: Saturday, October 16, 2010 08:17 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Joyce, THANK YOU for the link! :o) Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update? This is really going to help the "widget" count in my lab! My paths frequently want to test more than one block on their tumor cases and now I can charge for them! :o) Michelle -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Friday, October 15, 2010 10:45 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 http://www.flpath.org/rli2.asp Here is a link that will help.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" wrote: > > CMS/NCCI Update Dated October 1, 2009 > > 8. The unit of service for special stains (CPT codes 88312-88313) and > immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If > it is medically reasonable and necessary to perform the same stain on > more than one specimen or more than one block of tissue from the same > specimen, additional units of service may be reported for the > additional specimen(s) or block(s). Physicians should not report more > than one unit of service for a stain performed on a single tissue > block. For example it is common practice to cut multiple levels from a > tissue block and stain each level with the same stain. The multiple > levels from the same block of tissue stained with the same stain > should not be reported as additional units of service. Only one unit > of service should be reported for the stain on multiple levels from > the single tissue block. Additionally, controls performed with special > stains should not be reported as separate units of service for the > stain. > > > -----Original Message----- > From: Mike Pence [mailto:mpence@grhs.net] > Sent: Thursday, October 14, 2010 11:31 > To: Weems, Joyce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Can you site your source, please. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Thursday, October 14, 2010 10:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > > > > The change is that you can bill per block now and not per specimen. > This is for immunos and special stains. It does make a huge difference! > > Best, > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris > Evanish > Sent: Thursday, October 14, 2010 11:10 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 > > Has anyone heard of a cpt coding change that allows us to bill 88342 > per slide run instead of per antibody? One of our Pathologist was at a > conference and was told that we could do that. It makes a big > difference with running cytokeratins on multiple blocks and levels of > sentinel nodes. > > Thanks, > Chris Evanish > Montgomery Hospital > Norristown PA > > Chris D. Evanish > Histology Supervisor > Montgomery Hospital > 610-270-2379 > > Please consider the environment before printing this email " to your > outgoing mail. > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). It may contain information that is privileged and > confidential. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you are not the intended recipient, > please delete this message, and reply to the sender regarding the > error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/17/10 18:33:00 ------------------------------ Message: 4 Date: Sun, 17 Oct 2010 22:02:23 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] AFB To: "Cheryl Crowder" , Message-ID: <474B6E602A164B3C90484AFE22963783@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi - Can't find the original question - must have deleted it, so I'll answer through Cheryl's, if that's OK with her. If I remember, the question was something like - are there other microorganisms besides TB that stain with Kinyoun (or something like that). First, any mycobacterium (acid fast bacteria AFB) will stain with Ziehl-Neelsen, Kinyoun, Auramine-Rhodamine or Fites (hence anything staining with these stains is called AFB positive, meaning staining with these AF stains) . So that means Mycobacterium tuberculosis (TB), Mycobacterium avium intracellular (found in birds and I've seen it in spleen and bone marrow of immune suppressed patients), Mycobacterium paratuberculosis (found in intestine of infected cows and goats, causing something similar to Crone's disease), and a lot of other Mycobacterium are AFB positive staining with these procedures. Mycobacterium leprae (leprosy) is a very thin walled mycobacterium, so needs the Fites stain with the peanut oil (or any other oil, such as mineral oil) to coat the leprosy microorganism, so it can withstand decolorization with aqueous alcohol. But Fites can also be used to demonstrate any of the Mycobacterium. These AFB stains will also demonstrate the sulfur clubs of actinomyces, and the filamentous strands of nocardia are also weakly AFB positive. (Sort of bacteria with some fungus characteristics.) Cryptosporidium found in the intestine (causing severe diarrhea) can also stain AFB positive. And there are bacterium (mycobacterium or others) in tap water that are AFB positive, which can adhere to your slides from the flotation bath water, or from the tap water. Usually non-pathogenic AFB, but I don't know their names. And I'm sure there are other less common bacteria that I've forgotten or never known about that will stain AFB positive, which are not in the Mycobacterium genus. And let's remember that hair follicles, sperm (heads with lipids), lipofucsin (wear and tear pigment of lipids) also stain AFB positive. And also Russell bodies (inclusions of antibodies in plasma cells) will stain AFB positive. And keratin which is dense can simply require longer differentiation, or else it will retain the red color of the dye. So, yes, Amy, there are other things that stain positive with Kinyoun that are not TB/mycobacterium. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Cheryl Crowder" Sent: Saturday, October 16, 2010 10:48 AM To: Subject: [Histonet] AFB > Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast > bacteria. The Fite's stain is usually considered specific for > mycobacteria. > Cheryl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 18 Oct 2010 10:51:36 +0100 From: "Edwards, Richard E." Subject: [Histonet] autofluorescence To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8DB32C9DF@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Any information on current methods to counter autoflourescence in formalin fixed paraffin processed tissues would be much appreciated. Many thanks Richard Edwards Leicester University U.K................ ------------------------------ Message: 6 Date: Mon, 18 Oct 2010 08:13:07 -0500 From: Stephanie Rivera Subject: [Histonet] RE: IHC FILD To: Debora Probst , "histonet@lists.utsouthwestern.edu" Message-ID: <14A77A757E86BC499A34E86B47649B64054FC6BA7D@019D-NAMSG-05.019D.MGD.MSFT.NET> Content-Type: text/plain; charset="us-ascii" My company does not recognize the certification. It was just for my personal development. Some hospitals do recognize it as a specialty but I don't know if you get an increase. Check with your manager. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debora Probst Sent: Friday, October 15, 2010 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC FILD Can anyone tell me if once you have taken the IHC certification test and passed dose the administration consider that a specialty field and give you a pay increase? Or is it just for a persons own gratification to take it and pass? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 18 Oct 2010 10:51:08 -0400 From: "Debora Probst" Subject: [Histonet] (no subject) To: Message-ID: <4843CFFC8DC9A54E88E4AB3F5A50689819C1A93F@crhs_exch.nterprise.crhs.net> Content-Type: text/plain; charset="us-ascii" I would just like to thank everyone for their response back on IHC certification. What a great resource for our field (Histology). Again thank you everyone. Debora Probst Columbus Regional, Columbus Ga. ------------------------------ Message: 8 Date: Mon, 18 Oct 2010 07:53:00 -0700 From: Subject: [Histonet] Eosin To: histonet@lists.utsouthwestern.edu Message-ID: <20101018075300.9e2d9aa830e8449a2412eb1e4f2f067e.1df96a717f.wbe@email04.secureserver.net> Content-Type: text/plain; charset="utf-8" Once upon a time I heard that eosin fluoresces is this true? color does it show up? Could this be used as a sort of back gr stain so you can tell exactly how many cells are in the field? Sarah Goebel, B.A., HT (ASCP) Histotechnician 8201 East Riversid Austin, Texas (512)386-2907 ------------------------------ Message: 9 Date: Mon, 18 Oct 2010 10:04:23 -0500 From: "Bernice Frederick" Subject: [Histonet] Block release To: Message-ID: <0AD641BB647144BCBC1AE4CD68C18B22@lurie.northwestern.edu> Content-Type: text/plain; charset="US-ASCII" Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ------------------------------ Message: 10 Date: Mon, 18 Oct 2010 11:12:08 -0400 From: Helen Fedor Subject: RE: [Histonet] Block release To: Bernice Frederick , "histonet@lists.utsouthwestern.edu" Message-ID: <3201CF51728F6048A24FA3AFFFEEF1D31781F6FE65@JHEMTEXVS3.win.ad.jhu.edu> Content-Type: text/plain; charset="us-ascii" Hello, Our institution does release blocks, the requirement is that a patient consent is signed and we have an MTA (Material Transfer Agreement) For the study and appropriate IRB's in place for the study. It is a lot to set up, but after this is done for one study any following studies are easier to handle. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 18 Oct 2010 10:22:33 -0500 From: Subject: RE: [Histonet] Block release To: , Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA1395E57654@NADCWPMSGCMS03.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Good Morning to All, We, as often as possible, try not to release blocks for studies or legal cases. Most studies will let you submit 10-20 unstained recut slides on Plus slides. On legal cases, we always give recuts if there is enough tissue. When slides will not suffice, we will send the block and keep a record of the block being sent. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 18 Oct 2010 08:51:43 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Eosin To: histonet@lists.utsouthwestern.edu, sgoebel@xbiotech.com Message-ID: <9500.31952.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if you observe a solution with transmitted light. Ren? J. --- On Mon, 10/18/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: [Histonet] Eosin To: histonet@lists.utsouthwestern.edu Date: Monday, October 18, 2010, 10:53 AM ???Once? upon a time I heard that eosin fluoresces is this true? = ; What ???color? does it show up?? Could this be used as a sort of back gr= ound ???stain so you can tell exactly how many cells are in the field? ???Sarah Goebel, B.A., HT (ASCP) ???Histotechnician ???= = XBiotech USA Inc. ???8201 East Riversid= e Dr. Bldg 4 Suite 100 ???Austin, Texas=???78744 ???(512)386-2907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 18 Oct 2010 08:58:44 -0700 From: "Tench, Bill" Subject: [Histonet] clinical trial blocks To: histonet@lists.utsouthwestern.edu Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56FE@MAIL1.pph.local> Content-Type: text/plain; charset=us-ascii The fundamental philosophy that we have is that we do not want to stand in the way of the patient receiving any kind of treatment. That being said, we also have the policy that whether or not we "own" the material (patients are actually suppose to sign an admission consent that says they give up any ownership rights), we are clearly the legal custodians of the material, and as such are responsible for insuring that no irreparable distruction occurs. So, we prefer to not send out blocks unless the clilnical trial says there are no alternatives. We contact them and ask if we can send recuts from which they can obtain the information they desire. We also review what we have. If we have only one section, we cut a recut for the file before sending out any blocks. If we have only one block, with not much in it, we again discuss the issue with the trial coordinator. We do insist on return of the block, and assuming that there is an outside review of the material, we insist on a copy of the report. We also have the patient sign a release informing them that they need to understand that this process may exhaust any tissue that would be useful for some other new treatment which may come up down the line. Since we have to pull this material for review and usually make recuts, we bill the clinical trial. Years back, they never had any budget allowances for this, and it was always a hassle. More recently, we get a lot less BS. They know they have to pay, and they do. Unfortunately, nothing is free any more. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 83, Issue 29 **************************************** Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From ann.bennettann <@t> yahoo.com Mon Oct 18 16:24:24 2010 From: ann.bennettann <@t> yahoo.com (Ann Bennett) Date: Mon Oct 18 16:24:27 2010 Subject: [Histonet] Un related to actual histology Message-ID: <578742.8728.qm@web114319.mail.gq1.yahoo.com> Hello everyone! ? Our lab is having a laboratory related costume contest for Halloween.? I'd love to hear your ideas! From sgoebel <@t> xbiotech.com Mon Oct 18 16:28:59 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Oct 18 16:29:03 2010 Subject: [Histonet] Un related to actual histology Message-ID: <20101018142859.9e2d9aa830e8449a2412eb1e4f2f067e.a02f77a53b.wbe@email04.secureserver.net> I dressed up as Abby from CSI last year. Black wig, bright red lipstick, and a lab coat is all you need =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] Un related to actual histology From: Ann Bennett Date: Mon, October 18, 2010 2:24 pm To: histonet@lists.utsouthwestern.edu Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Mon Oct 18 16:55:09 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Oct 18 16:55:20 2010 Subject: [Histonet] Un related to actual histology Message-ID: <20101018145509.9e2d9aa830e8449a2412eb1e4f2f067e.4d7bcb59bf.wbe@email04.secureserver.net> Actually it's NCIS... =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: RE: [Histonet] Un related to actual histology From: Date: Mon, October 18, 2010 2:28 pm To: "Ann Bennett" Cc: histonet@lists.utsouthwestern.edu I dressed up as Abby from CSI last year. Black wig, bright red lipstick, and a lab coat is all you need =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] Un related to actual histology From: Ann Bennett Date: Mon, October 18, 2010 2:24 pm To: histonet@lists.utsouthwestern.edu Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Mon Oct 18 17:37:03 2010 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Oct 18 17:37:11 2010 Subject: [Histonet] Un related to actual histology In-Reply-To: <578742.8728.qm@web114319.mail.gq1.yahoo.com> References: <578742.8728.qm@web114319.mail.gq1.yahoo.com> Message-ID: I don't know if you're a fan of Mystery Science Theater 3000, but you could be Dr Clayton Forrester! Or you could be Dexter or House. Dexter would be fun. Better yet, be Frankenstein, the doctor. That'll confuse people who aren't aware the monster had no name. Or even, FrankenSTEEN. Stab a scapel in your thigh for full effect. Also bring a recording of horses neighing so you can say "Frau Bleuker!" Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Mon, Oct 18, 2010 at 5:24 PM, Ann Bennett wrote: > Hello everyone! > > Our lab is having a laboratory related costume contest for Halloween. I'd > love to hear your ideas! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CIngles <@t> uwhealth.org Mon Oct 18 18:56:01 2010 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Mon Oct 18 18:56:06 2010 Subject: [Histonet] Un related to actual histology References: <578742.8728.qm@web114319.mail.gq1.yahoo.com> Message-ID: Ah---A woman after my own heart. I always thought of going as Bunson Honeydew or (especially) Beaker from the muppet show. I work in a clinic setting and have always thought of coming in as the nurse/ Daryl Hannah character from Kill Bill would be interesting, but I don't want to give the patients heart attacks or have all the nurses get offended and get me fired. I would also of course bring a tape recorder to play her theme as well as wearing the obligatory katana. Or even, FrankenSTEEN. Stab a scapel in your thigh for full effect. Also bring a recording of horses neighing so you can say "Frau Bleuker!" Emily -- From deannal78 <@t> verizon.net Mon Oct 18 20:42:45 2010 From: deannal78 <@t> verizon.net (Deanna Rhoads) Date: Mon Oct 18 20:42:48 2010 Subject: [Histonet] Un related to actual histology In-Reply-To: References: <578742.8728.qm@web114319.mail.gq1.yahoo.com> Message-ID: <487658.27019.qm@web84306.mail.re1.yahoo.com> They have some really neat Dexter costumes on Showtime's website!? I'm so upset that I just discovered Dexter!! I'm addicted :o) I like the Abby costume idea too. ________________________________ From: Emily Sours To: Ann Bennett ; histonet@lists.utsouthwestern.edu Sent: Mon, October 18, 2010 6:37:03 PM Subject: Re: [Histonet] Un related to actual histology I don't know if you're a fan of Mystery Science Theater 3000, but you could be Dr Clayton Forrester! Or you could be Dexter or House.? Dexter would be fun. Better yet, be Frankenstein, the doctor.? That'll confuse people who aren't aware the monster had no name. Or even, FrankenSTEEN.? Stab a scapel in your thigh for full effect.? Also bring a recording of horses neighing so you can say "Frau Bleuker!" Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Mon, Oct 18, 2010 at 5:24 PM, Ann Bennett wrote: > Hello everyone! > > Our lab is having a laboratory related costume contest for Halloween.? I'd > love to hear your ideas! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Tue Oct 19 02:34:24 2010 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Tue Oct 19 02:34:43 2010 Subject: [Histonet] Eosin In-Reply-To: <9500.31952.qm@web65703.mail.ac4.yahoo.com> References: <9500.31952.qm@web65703.mail.ac4.yahoo.com> Message-ID: <20592.1302.qm@web29602.mail.ird.yahoo.com> Yes. Especially when you watch into a test tube an 1% eosin solution and it is going to be diluted with distilled water. At that point it can be used for the staining of histological slices. Best Regards, Massimo ________________________________ Da: Rene J Buesa A: histonet@lists.utsouthwestern.edu; sgoebel@xbiotech.com Inviato: Lun 18 ottobre 2010, 17:51:43 Oggetto: Re: [Histonet] Eosin Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if you observe a solution with transmitted light. Ren? J. --- On Mon, 10/18/10, sgoebel@xbiotech.com wrote: From: sgoebel@xbiotech.com Subject: [Histonet] Eosin To: histonet@lists.utsouthwestern.edu Date: Monday, October 18, 2010, 10:53 AM Once upon a time I heard that eosin fluoresces is this true? = ; What color does it show up? Could this be used as a sort of back gr= ound stain so you can tell exactly how many cells are in the field? Sarah Goebel, B.A., HT (ASCP) Histotechnician = = XBiotech USA Inc. 8201 East Riversid= e Dr. Bldg 4 Suite 100 Austin, Texas= 78744 (512)386-2907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MMargiotta <@t> bmhmc.org Tue Oct 19 07:14:17 2010 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Tue Oct 19 07:14:22 2010 Subject: [Histonet] hi-profile blades Message-ID: Hi All, One of my techs loves using hi-profile blades but recently she has been having problems with the quality and consistency of the blades. One will cut great and the next one will give her problems cutting. I'd appreciate any suggestions for blade type/company that histotechs are happy with so we can try them out. Thanks, Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From WBRID <@t> capefearvalley.com Tue Oct 19 07:18:43 2010 From: WBRID <@t> capefearvalley.com (Wendy Bridges) Date: Tue Oct 19 07:18:48 2010 Subject: [Histonet] hi-profile blades Message-ID: I have always found Accu-Cut blades by Sakura to be the best blades. Wendy Bridges, HT (ASCP) Lead Tech Specialist Cape Fear Valley Hospital Fayetteville, NC CONFIDENTIALITY NOTICE: This electronic mail transmission may contain information that is privileged and/or confidential. Additionally, this communication may contain individual protected health information ("PHI") that is subject to protection under state and federal laws, or other privileged, confidential or proprietary information of Cape Fear Valley Health System that may not be further disclosed. Please be advised that any disclosure, copying, distribution or other use of the contents of this message by anyone other than the intended recipient is prohibited. If you have received this communication in error, please notify the sender immediately by replying to the message and deleting it from your computer. From jtaramona70 <@t> gmail.com Tue Oct 19 08:15:39 2010 From: jtaramona70 <@t> gmail.com (Jose Taramona) Date: Tue Oct 19 08:15:42 2010 Subject: [Histonet] 2 Histoech positions open at PBM Dallas Tx Message-ID: Looking for 2 excellent, reliable histotechs for routine histology department. Also looking for an excellent histotech with Immuno experience. Competitive pay. Interested? Need more information contact Jose Taramona at: Email: joset@pbmlabs.com Ph# 214-818-9138 Fax# 214-818-9170 attn: Jose From sgoebel <@t> xbiotech.com Tue Oct 19 08:49:57 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Oct 19 08:50:02 2010 Subject: [Histonet] Un related to actual histology Message-ID: <20101019064957.9e2d9aa830e8449a2412eb1e4f2f067e.f20541e76b.wbe@email04.secureserver.net> Roll Roll Roll in the hay =) One of my all time favorite movies!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] Un related to actual histology From: Emily Sours Date: Mon, October 18, 2010 3:37 pm To: Ann Bennett , histonet@lists.utsouthwestern.edu I don't know if you're a fan of Mystery Science Theater 3000, but you could be Dr Clayton Forrester! Or you could be Dexter or House. Dexter would be fun. Better yet, be Frankenstein, the doctor. That'll confuse people who aren't aware the monster had no name. Or even, FrankenSTEEN. Stab a scapel in your thigh for full effect. Also bring a recording of horses neighing so you can say "Frau Bleuker!" Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Mon, Oct 18, 2010 at 5:24 PM, Ann Bennett wrote: > Hello everyone! > > Our lab is having a laboratory related costume contest for Halloween. I'd > love to hear your ideas! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Kuhnla <@t> chsli.org Tue Oct 19 09:49:56 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Tue Oct 19 09:50:05 2010 Subject: [Histonet] kappa and lambda by CISH Message-ID: Hello All, Anyone currently running Kappa and Lambda by CISH? I am in the process of validating the Ventana products. Due to the ASR status of these products the vendor is extremely 'tight lipped'. I have the stains worked up and have completed a 25 case validation. Does anyone know if this test is not to be used on decaled specimens?? Thank you The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From mhale <@t> carisls.com Tue Oct 19 09:53:38 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Oct 19 09:53:44 2010 Subject: [Histonet] TN - Great HT Opprotunity Message-ID: <6F33D8418806044682A391273399860F059AFAEA@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From Kim.Donadio <@t> bhcpns.org Tue Oct 19 10:04:54 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Oct 19 10:05:02 2010 Subject: [Histonet] DAKO Coverslipper Message-ID: Has anyone ever used the Dako coverslipper? If so, what did you think? Thanks Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Oct 19 10:04:49 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Oct 19 10:06:12 2010 Subject: [Histonet] RE: kappa and lambda by CISH In-Reply-To: References: Message-ID: I have been using the RISH from Biocare and you can use it on Decaled specimens. they have a slightly different protocol (pretreatment) but we have found that you don't need to adjust with our form of decal. Not completely finished with the validation process on these, but that is what we have found so far. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa [Melissa.Kuhnla@chsli.org] Sent: Tuesday, October 19, 2010 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] kappa and lambda by CISH Hello All, Anyone currently running Kappa and Lambda by CISH? I am in the process of validating the Ventana products. Due to the ASR status of these products the vendor is extremely 'tight lipped'. I have the stains worked up and have completed a 25 case validation. Does anyone know if this test is not to be used on decaled specimens?? Thank you The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Oct 19 10:39:53 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Oct 19 10:40:02 2010 Subject: AW: [Histonet] kappa and lambda by CISH In-Reply-To: References: Message-ID: <0353E09776024B15A11DD4B5DC7F738F@dielangs.at> I've found, that kappa and lambda mRNA CISH is reliable for specimen decalcified with 5-10% formic acid. Decal with harsher acids like hydrochloric acid should be validated, because any acid will cause degradation of nucleic acids. We use the CISH kit from Zytovision. Journal of Histotechnol 33(1):9-13, 2010 Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kuhnla, Melissa Gesendet: Dienstag, 19. Oktober 2010 16:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] kappa and lambda by CISH Hello All, Anyone currently running Kappa and Lambda by CISH? I am in the process of validating the Ventana products. Due to the ASR status of these products the vendor is extremely 'tight lipped'. I have the stains worked up and have completed a 25 case validation. Does anyone know if this test is not to be used on decaled specimens?? Thank you The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Oct 19 10:44:12 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Oct 19 10:44:45 2010 Subject: [Histonet] hi-profile blades In-Reply-To: Message-ID: <67893F847B3D4CB9848EB1763C8F2ECD@lurie.northwestern.edu> I've been using Accu-edge blades from Sakura and have found nothing to equal them. We use low profile here, but high are the same quality. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Tuesday, October 19, 2010 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hi-profile blades Hi All, One of my techs loves using hi-profile blades but recently she has been having problems with the quality and consistency of the blades. One will cut great and the next one will give her problems cutting. I'd appreciate any suggestions for blade type/company that histotechs are happy with so we can try them out. Thanks, Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 41dmb41 <@t> gmail.com Tue Oct 19 10:47:49 2010 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Tue Oct 19 10:48:14 2010 Subject: [Histonet] hi-profile blades In-Reply-To: <67893F847B3D4CB9848EB1763C8F2ECD@lurie.northwestern.edu> References: <67893F847B3D4CB9848EB1763C8F2ECD@lurie.northwestern.edu> Message-ID: I agree with Bernice... I've used both the low and high profile Sakura AccuEdge.... nothing comes close to their quality and consistency. Drew On Tue, Oct 19, 2010 at 11:44, Bernice Frederick < b-frederick@northwestern.edu> wrote: > I've been using Accu-edge blades from Sakura and have found nothing to > equal > them. We use low profile here, but high are the same quality. > > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > ECOGPCO-RL > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, > Michele > Sent: Tuesday, October 19, 2010 7:14 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] hi-profile blades > > Hi All, > > One of my techs loves using hi-profile blades but recently she has been > having problems with the quality and consistency of the blades. One will > cut great and the next one will give her problems cutting. I'd appreciate > any suggestions for blade type/company that histotechs are happy with so we > can try them out. > > Thanks, > Michele Margiotta > BMHMC > Histology Supervisor > 631-654-7192 > > > > > DISCLAIMER: > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they are > addressed. This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or the > person responsible for delivering the e-mail to the intended recipient, be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. If you have received this e-mail in error, please immediately > notify the sender via return e-mail or call Brookhaven Memorial Hospital > Medical Center at (631) 654-7282. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From marktarango <@t> gmail.com Tue Oct 19 10:48:32 2010 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Oct 19 10:48:40 2010 Subject: [Histonet] kappa and lambda by CISH In-Reply-To: References: Message-ID: Hi Melissa, We use it on bone marrow biopsies all the time on the Ventana XT. We made sure to include BMBXs in the validation. We do get strange staining from time to time, so it's particularily important to look at the negative control for these. Mark Tarango On Tue, Oct 19, 2010 at 7:49 AM, Kuhnla, Melissa wrote: > Hello All, > Anyone currently running Kappa and Lambda by CISH? I am in the process > of validating the Ventana products. Due to the ASR status of these > products the vendor is extremely 'tight lipped'. I have the stains > worked up and have completed a 25 case validation. Does anyone know if > this test is not to be used on decaled specimens?? > Thank you > > > The information in this e-mail, and any attachments therein, is > confidential and for use by the intended addressee only. If this message is > received by you in error please do not disseminate or read further. Please > reply to the sender that you have received the message in error, then delete > the message. Although Catholic Health Services of Long Island attempts to > sweep e-mail and attachments for viruses, it does not guarantee that either > are virus-free and accepts no liability for any damage sustained as a result > of viruses. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AGleiberman <@t> cbiolabs.com Tue Oct 19 11:00:01 2010 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue Oct 19 11:00:06 2010 Subject: [Histonet] hi-profile blades In-Reply-To: References: <67893F847B3D4CB9848EB1763C8F2ECD@lurie.northwestern.edu> Message-ID: We use AccuEdge low and high profile from Sakura and several low and high profile blades from Sturkey (Silver, Gold and Extremus) and found that Sturkey knifes are as good as AccuEdge (both for paraffin and cryo sections) and significantly cheaper if you buy it directly from the company. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, October 19, 2010 11:48 AM To: Bernice Frederick Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] hi-profile blades I agree with Bernice... I've used both the low and high profile Sakura AccuEdge.... nothing comes close to their quality and consistency. Drew On Tue, Oct 19, 2010 at 11:44, Bernice Frederick < b-frederick@northwestern.edu> wrote: > I've been using Accu-edge blades from Sakura and have found nothing to > equal > them. We use low profile here, but high are the same quality. > > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > ECOGPCO-RL > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, > Michele > Sent: Tuesday, October 19, 2010 7:14 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] hi-profile blades > > Hi All, > > One of my techs loves using hi-profile blades but recently she has been > having problems with the quality and consistency of the blades. One will > cut great and the next one will give her problems cutting. I'd appreciate > any suggestions for blade type/company that histotechs are happy with so we > can try them out. > > Thanks, > Michele Margiotta > BMHMC > Histology Supervisor > 631-654-7192 > > > > > DISCLAIMER: > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they are > addressed. This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or the > person responsible for delivering the e-mail to the intended recipient, be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. If you have received this e-mail in error, please immediately > notify the sender via return e-mail or call Brookhaven Memorial Hospital > Medical Center at (631) 654-7282. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From STACEY.LANGENBERG <@t> UCDENVER.EDU Tue Oct 19 11:21:34 2010 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Tue Oct 19 11:21:52 2010 Subject: [Histonet] hi-profile blades In-Reply-To: References: <67893F847B3D4CB9848EB1763C8F2ECD@lurie.northwestern.edu> Message-ID: <355579523.73469.1287505304394.JavaMail.rim@bda341.bisx.prod.on.blackberry> I too swear by Sturkey. It is the only blade my lab buys and uses. My techs could use any blade they want but they all love Sturkey! Stacey Langenberg Sent via BlackBerry from T-Mobile -----Original Message----- From: Anatoli Gleiberman Sender: "histonet-bounces@lists.utsouthwestern.edu" Date: Tue, 19 Oct 2010 10:00:01 To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] hi-profile blades We use AccuEdge low and high profile from Sakura and several low and high profile blades from Sturkey (Silver, Gold and Extremus) and found that Sturkey knifes are as good as AccuEdge (both for paraffin and cryo sections) and significantly cheaper if you buy it directly from the company. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, October 19, 2010 11:48 AM To: Bernice Frederick Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] hi-profile blades I agree with Bernice... I've used both the low and high profile Sakura AccuEdge.... nothing comes close to their quality and consistency. Drew On Tue, Oct 19, 2010 at 11:44, Bernice Frederick < b-frederick@northwestern.edu> wrote: > I've been using Accu-edge blades from Sakura and have found nothing to > equal > them. We use low profile here, but high are the same quality. > > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > ECOGPCO-RL > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, > Michele > Sent: Tuesday, October 19, 2010 7:14 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] hi-profile blades > > Hi All, > > One of my techs loves using hi-profile blades but recently she has been > having problems with the quality and consistency of the blades. One will > cut great and the next one will give her problems cutting. I'd appreciate > any suggestions for blade type/company that histotechs are happy with so we > can try them out. > > Thanks, > Michele Margiotta > BMHMC > Histology Supervisor > 631-654-7192 > > > > > DISCLAIMER: > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they are > addressed. This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or the > person responsible for delivering the e-mail to the intended recipient, be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. If you have received this e-mail in error, please immediately > notify the sender via return e-mail or call Brookhaven Memorial Hospital > Medical Center at (631) 654-7282. > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Oct 19 11:45:29 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 19 11:45:34 2010 Subject: [Histonet] CLIA Inspection Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16403A6810E31@CHEXCMS10.one.ads.che.org> Would someone who has been CLIA inspected, please contact me privately? Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jcampbell_mdl <@t> frontier.com Tue Oct 19 12:16:13 2010 From: jcampbell_mdl <@t> frontier.com (Jen Campbell) Date: Tue Oct 19 12:16:18 2010 Subject: [Histonet] Fwd: IHC coverglassing In-Reply-To: <1516815007.661479.1287501398604.JavaMail.root@cl02-host03.roch.ny.frontiernet.net> Message-ID: <1138837970.663741.1287508572943.JavaMail.root@cl02-host03.roch.ny.frontiernet.net> Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 ----- Forwarded Message ----- From: "Jen Campbell" To: "histonet" Sent: Tuesday, October 19, 2010 11:16:38 AM Subject: IHC coverglassing Greetings to all the histonet followers!! A question was recently brought forth in the lab I work at. Can "non-automated" IHC slides be dehydrated and cleared(xylene substitute) after staining and be placed on an automated coverglasser? Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 From HParker <@t> Skaggs.Net Tue Oct 19 12:18:03 2010 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Tue Oct 19 12:18:07 2010 Subject: [Histonet] High Profile Blades In-Reply-To: <0c41fd36-be5b-42c7-86fb-571fa4c0a159@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F5060045F4136CB@email1.skaggs.net> I used to love the TBS blades (green- I think), but we use low profile here. We use Thermo and Accu Edge. Helayne Parker CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From Maria.Katleba <@t> stjoe.org Tue Oct 19 12:27:34 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Oct 19 12:27:45 2010 Subject: [Histonet] Frozen sections In-Reply-To: <20101018145509.9e2d9aa830e8449a2412eb1e4f2f067e.4d7bcb59bf.wbe@email04.secureserver.net> References: <20101018145509.9e2d9aa830e8449a2412eb1e4f2f067e.4d7bcb59bf.wbe@email04.secureserver.net> Message-ID: Question: When doing frozen section on a lung mass (not fatty- just regular), and it chatters, what is the cause? The cryostat was set at -26, we used the normal OCT media, and cut at 5microns..... I prefer not so cold (like -23 to -24) and I cut sections at 4, 5 , and even 6 to see if it would help... still chatter! The pathologist said it looked all necrotic-like!! Without knowing for sure if the tissue's morphology is due to its real state (ie- full of cancer) or a cause of freezing artifact, I need some help from any histotech that is a Frozen Section Guru :) Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From nto <@t> stowers.org Tue Oct 19 12:41:15 2010 From: nto <@t> stowers.org (Thomas, Nancy) Date: Tue Oct 19 12:41:23 2010 Subject: [Histonet] Fwd: IHC coverglassing In-Reply-To: <1138837970.663741.1287508572943.JavaMail.root@cl02-host03.roch.ny.frontiernet.net> Message-ID: Hi Jen, We do it that way routinely. No problems. Nancy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jen Campbell Sent: Tuesday, October 19, 2010 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: IHC coverglassing Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 ----- Forwarded Message ----- From: "Jen Campbell" To: "histonet" Sent: Tuesday, October 19, 2010 11:16:38 AM Subject: IHC coverglassing Greetings to all the histonet followers!! A question was recently brought forth in the lab I work at. Can "non-automated" IHC slides be dehydrated and cleared(xylene substitute) after staining and be placed on an automated coverglasser? Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Tue Oct 19 12:49:37 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Oct 19 12:50:10 2010 Subject: [Histonet] Fwd: IHC coverglassing In-Reply-To: <1138837970.663741.1287508572943.JavaMail.root@cl02-host03.roch.ny.frontiernet.net> References: <1516815007.661479.1287501398604.JavaMail.root@cl02-host03.roch.ny.frontiernet.net> <1138837970.663741.1287508572943.JavaMail.root@cl02-host03.roch.ny.frontiernet.net> Message-ID: absolutely Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jen Campbell Sent: Tuesday, October 19, 2010 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: IHC coverglassing Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 ----- Forwarded Message ----- From: "Jen Campbell" To: "histonet" Sent: Tuesday, October 19, 2010 11:16:38 AM Subject: IHC coverglassing Greetings to all the histonet followers!! A question was recently brought forth in the lab I work at. Can "non-automated" IHC slides be dehydrated and cleared(xylene substitute) after staining and be placed on an automated coverglasser? Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From sgoebel <@t> xbiotech.com Tue Oct 19 13:00:40 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Oct 19 13:00:46 2010 Subject: [Histonet] Fwd: IHC coverglassing Message-ID: <20101019110040.9e2d9aa830e8449a2412eb1e4f2f067e.ee29d6c038.wbe@email04.secureserver.net> Absolutely!! After the buffer rinse (after the counterstain), rinse in water, then 95, then 100, then xylene, and coverslip away!! I do it everyday and it works fine =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] Fwd: IHC coverglassing From: Jen Campbell Date: Tue, October 19, 2010 10:16 am To: histonet@lists.utsouthwestern.edu Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 ----- Forwarded Message ----- From: "Jen Campbell" To: "histonet" Sent: Tuesday, October 19, 2010 11:16:38 AM Subject: IHC coverglassing Greetings to all the histonet followers!! A question was recently brought forth in the lab I work at. Can "non-automated" IHC slides be dehydrated and cleared(xylene substitute) after staining and be placed on an automated coverglasser? Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Tue Oct 19 13:01:57 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Tue Oct 19 13:02:03 2010 Subject: [Histonet] FX Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5710@MAIL1.pph.local> it's too cold. set your cryostat at 20. put your tumb on the block Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From sgoebel <@t> xbiotech.com Tue Oct 19 13:03:32 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Oct 19 13:03:37 2010 Subject: [Histonet] Frozen sections Message-ID: <20101019110332.9e2d9aa830e8449a2412eb1e4f2f067e.4fcc676645.wbe@email04.secureserver.net> Chatter can also be caused by your blade angle. Try making the angle more obtuse, just don't over do it or you will get venitian (sp?) blind. The temperature is also a little cold. Try putting your thumb on the chuck, on top of the tissue, for a few sections before cutting and see if that helps any. If the tissue is necrotic, it's kind of like fat...not much you can do? Good Luck!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] Frozen sections From: Maria Katleba Date: Tue, October 19, 2010 10:27 am To: "histonet@lists.utsouthwestern.edu" Question: When doing frozen section on a lung mass (not fatty- just regular), and it chatters, what is the cause? The cryostat was set at -26, we used the normal OCT media, and cut at 5microns..... I prefer not so cold (like -23 to -24) and I cut sections at 4, 5 , and even 6 to see if it would help... still chatter! The pathologist said it looked all necrotic-like!! Without knowing for sure if the tissue's morphology is due to its real state (ie- full of cancer) or a cause of freezing artifact, I need some help from any histotech that is a Frozen Section Guru :) Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Tue Oct 19 13:05:14 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Tue Oct 19 13:05:19 2010 Subject: [Histonet] fx Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5712@MAIL1.pph.local> that's suppose to be put your "thumb" on the block. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From JMacDonald <@t> mtsac.edu Tue Oct 19 13:12:31 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Oct 19 13:12:41 2010 Subject: [Histonet] hi-profile blades In-Reply-To: Message-ID: Is the inconsistency between brands of blades, the same brand, or within an individual dispenser? "Margiotta, Michele" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/19/2010 05:18 AM To cc Subject [Histonet] hi-profile blades Hi All, One of my techs loves using hi-profile blades but recently she has been having problems with the quality and consistency of the blades. One will cut great and the next one will give her problems cutting. I'd appreciate any suggestions for blade type/company that histotechs are happy with so we can try them out. Thanks, Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbrooks <@t> incytepathology.com Tue Oct 19 13:20:02 2010 From: mbrooks <@t> incytepathology.com (Matt Brooks) Date: Tue Oct 19 13:20:07 2010 Subject: [Histonet] Tissue Processors Message-ID: <706224670091FE47997AEF88EFADE7CA0194E9B1@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello All, I am in the process of developing the Histology budget for next three years. There have been some positive posts about a few processors recently; but if there are more opinions out there (and I know that there are) please let me know. At NSH I had the opportunity to view and hear about the most of the tissue processors on the market. There are several features that I need to consider price, reliability, ease of use/maintenance, timely and quality service, and processing TAT, just to name a few. Fellow Histology professionals you can email/contact me directly and your input is greatly appreciated. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 From NSEARCY <@t> swmail.sw.org Tue Oct 19 13:22:21 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Oct 19 13:22:30 2010 Subject: [Histonet] Cassette Marking Message-ID: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From Kim.Donadio <@t> bhcpns.org Tue Oct 19 13:25:39 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Oct 19 13:25:47 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> Message-ID: IF I had to. pencil. And I am very funny about making sure it has a nice sharp dark lead. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Nita Searcy" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/19/2010 01:22 PM To cc Subject [Histonet] Cassette Marking ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Kim.Donadio <@t> bhcpns.org Tue Oct 19 13:28:42 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Oct 19 13:28:46 2010 Subject: [Histonet] FX In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5710@MAIL1.pph.local> Message-ID: I agree. -20 is good Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Tench, Bill" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/19/2010 01:01 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] FX it's too cold. set your cryostat at 20. put your tumb on the block Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Maria.Katleba <@t> stjoe.org Tue Oct 19 13:34:04 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Oct 19 13:34:22 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> Message-ID: Try www.MercedesMedical.com item # MER MARKER called Platinum Marker These pens are dark and keep their colour, pens outlast the Secureline marker and this company is very competitive with the price of this marker.... Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From KSly <@t> cmch.org Tue Oct 19 13:35:44 2010 From: KSly <@t> cmch.org (Karen Sly) Date: Tue Oct 19 13:36:40 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> Message-ID: <4CBDACBF.BAF2.00B1.0@cmch.org> We use Cancer Diagnostics, Moist Mark Plus. Karen Sly Histology Dept. Central Michigan Community Hospital Mount Pleasant, Michigan Please consider the environment before printing this e-mail Please consider the environment before printing this e-mail From MSHERWOOD <@t> PARTNERS.ORG Tue Oct 19 13:38:06 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Oct 19 13:38:10 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5110@PHSXMB30.partners.org> The marking pencils work fine and we just ordered some marking pens from StatLab which work great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Maria.Katleba <@t> stjoe.org Tue Oct 19 13:42:56 2010 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Oct 19 13:43:10 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5110@PHSXMB30.partners.org> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5110@PHSXMB30.partners.org> Message-ID: Yes- I love Statlab! These marking pencils are great Maria -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, October 19, 2010 11:38 AM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette Marking The marking pencils work fine and we just ordered some marking pens from StatLab which work great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Jacqueline.Farnsworth <@t> cls.ab.ca Tue Oct 19 13:42:44 2010 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Tue Oct 19 13:43:42 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5110@PHSXMB30.partners.org> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org>, <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5110@PHSXMB30.partners.org> Message-ID: Surgipath 01880 Permanent Ink . Ultra fine point. Non-toxic Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret [MSHERWOOD@PARTNERS.ORG] Sent: October 19, 2010 12:38 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette Marking The marking pencils work fine and we just ordered some marking pens from StatLab which work great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From sgoebel <@t> xbiotech.com Tue Oct 19 13:47:57 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Oct 19 13:48:02 2010 Subject: [Histonet] Cassette Marking Message-ID: <20101019114757.9e2d9aa830e8449a2412eb1e4f2f067e.d96b9e52a7.wbe@email04.secureserver.net> I've used this one too, the tip is very tiny, but the pen is awesome! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: RE: [Histonet] Cassette Marking From: Maria Katleba Date: Tue, October 19, 2010 11:34 am To: Nita Searcy , "histonet@lists.utsouthwestern.edu" Try www.MercedesMedical.com item # MER MARKER called Platinum Marker These pens are dark and keep their colour, pens outlast the Secureline marker and this company is very competitive with the price of this marker.... Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Tue Oct 19 13:49:51 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Oct 19 13:49:55 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5112@PHSXMB30.partners.org> Nita, I would say you have quite a choice! For the longest time, we had so much trouble with so many pens, we went to the pencil for the cassettes which are the best. But we still needed pens for the slides; that's when we switched to StatLab. We still use the pencil for the cassettes. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From KJohnson <@t> med.miami.edu Tue Oct 19 13:50:08 2010 From: KJohnson <@t> med.miami.edu (Johnson, Kevin) Date: Tue Oct 19 13:50:14 2010 Subject: [Histonet] Plain Vanilla Autostainer? Message-ID: <59F7809368E0084C8AE6B38C93D735EB0924579D77@MEDEXMB02.ad.med.miami.edu> I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL From sgoebel <@t> xbiotech.com Tue Oct 19 14:05:55 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Oct 19 14:05:59 2010 Subject: [Histonet] Plain Vanilla =?UTF-8?Q?Autostainer=3F?= Message-ID: <20101019120555.9e2d9aa830e8449a2412eb1e4f2f067e.856c9f4fda.wbe@email04.secureserver.net> What about just a simple old school ski stainer? Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] Plain Vanilla Autostainer? From: "Johnson, Kevin" Date: Tue, October 19, 2010 11:50 am To: "'histonet@lists.utsouthwestern.edu'" I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Tue Oct 19 14:30:15 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Tue Oct 19 14:30:39 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5112@PHSXMB30.partners.org> Message-ID: Yes, that's the reason we chose pencil's. We would have so many different marking pens around, some would wash off or smudge. One of the bad ones would end up in a drawer and get used later on down the line giving us a major headache. So pencil it is if we ever needed to. We have a cassette marker. But if it broke the handy dandy pencil would be our sure known tool of choice. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Sherwood, Margaret " Sent by: histonet-bounces@lists.utsouthwestern.edu 10/19/2010 01:49 PM To "Nita Searcy" , cc Subject RE: [Histonet] Cassette Marking Nita, I would say you have quite a choice! For the longest time, we had so much trouble with so many pens, we went to the pencil for the cassettes which are the best. But we still needed pens for the slides; that's when we switched to StatLab. We still use the pencil for the cassettes. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From hymclab.hymclab <@t> ministryhealth.org Tue Oct 19 14:49:18 2010 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Tue Oct 19 14:49:26 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5110@PHSXMB30.partners.org> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5110@PHSXMB30.partners.org> Message-ID: We use the pens from Statlab also and love them. Dawn D. Schneider, HT(ASCP) Lead Histology Tech Howard Young Medical Center 240 Maple St. Woodruff, WI 54568 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, October 19, 2010 1:38 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette Marking The marking pencils work fine and we just ordered some marking pens from StatLab which work great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From gayle.callis <@t> bresnan.net Tue Oct 19 15:06:29 2010 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Oct 19 15:06:38 2010 Subject: [Histonet] Autofluorescence and literature for getting rid of the problem Message-ID: <006501cb6fc9$20059ee0$6010dca0$@callis@bresnan.net> Richard, You will find what you need in a free pdf on autofluorescence from Wright Imaging Facility in Toronto Canada. They have a website, then download Autofluorescence: Causes and Cures. Also, if you can't get rid of autofluorescence , use a near infrared fluorophore e.g. Alexa 750. There is no autofluorescence seen in the NIR range. Just Google the title and it will come up instantly. They also have a pdf on Mounting Media for fluorescence work. For the little FFPE fluorescent work we do, you can try 100 to 300 mM glycine in TRIS buffer pH 7.4 for 20 to 30 minutes before embarking on immunostaining. Glycine binds free aldehydes to reduce the autofluorescence but doesn't always work 100%. It may reduce the problem but not totally eliminate it. There are other references on getting rid of autofluorescence, one for GFP, a review, but it applies to FFPE tissue even not containing GFP. I will be happy to send the pdf if you wish. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From smcbride <@t> andrew.cmu.edu Tue Oct 19 15:38:33 2010 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Tue Oct 19 15:38:41 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> Message-ID: Nita, We use HistoTec pens by Newcomer Supply ~Sean On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > ** High Priority ** > > If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? > > Anything else on the market? > > Thanks > > > > Nita Searcy, HT/HTL (ASCP) > Scott and White Hospital > Division Manager, Anatomic Pathology > 2401 S. 31st. Street > 254-724-2438 > Temple, Texas, 76502 > nsearcy@swmail.sw.org > > > 254-724-2438 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Oct 19 15:56:58 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Oct 19 15:59:54 2010 Subject: [Histonet] Cassette Marking In-Reply-To: References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> Message-ID: <4CBE061A.7070702@pathology.washington.edu> I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: > Nita, > > We use HistoTec pens by Newcomer Supply > > ~Sean > > > On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > >> ** High Priority ** >> >> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? >> >> Anything else on the market? >> >> Thanks >> >> >> >> Nita Searcy, HT/HTL (ASCP) >> Scott and White Hospital >> Division Manager, Anatomic Pathology >> 2401 S. 31st. Street >> 254-724-2438 >> Temple, Texas, 76502 >> nsearcy@swmail.sw.org >> >> >> 254-724-2438 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Tue Oct 19 16:04:22 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Oct 19 16:04:27 2010 Subject: [Histonet] Cassette Marking Message-ID: <20101019140422.9e2d9aa830e8449a2412eb1e4f2f067e.54130242e4.wbe@email04.secureserver.net> As compared to a lawsuit, yes it's cheaper. But, when trying to explain to a budget committee that you need something to label things that you can do by hand...they usually don't see the point. This brings me back to my original point of mislabelling things 2 times in a year... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] Cassette Marking From: Victor Tobias Date: Tue, October 19, 2010 1:56 pm To: histonet@lists.utsouthwestern.edu I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: > Nita, > > We use HistoTec pens by Newcomer Supply > > ~Sean > > > On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > >> ** High Priority ** >> >> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? >> >> Anything else on the market? >> >> Thanks >> >> >> >> Nita Searcy, HT/HTL (ASCP) >> Scott and White Hospital >> Division Manager, Anatomic Pathology >> 2401 S. 31st. Street >> 254-724-2438 >> Temple, Texas, 76502 >> nsearcy@swmail.sw.org >> >> >> 254-724-2438 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos <@t> allergydermatology.com Tue Oct 19 15:26:01 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Tue Oct 19 16:09:47 2010 Subject: [Histonet] Cassette Marking In-Reply-To: References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org>, <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5110@PHSXMB30.partners.org> Message-ID: <000f01cb6fcb$dad7efa0$9087cee0$@com> Most places will also send you a sample. This way you can run test blocks in your processor to make sure you are happy w/ the results. Just remember to label the block w/ the pen name so you wont get confused! :) I currently use Mercedes Platinum Marker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqueline Farnsworth Sent: Tuesday, October 19, 2010 11:43 AM To: Sherwood, Margaret ; Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette Marking Surgipath 01880 Permanent Ink . Ultra fine point. Non-toxic Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret [MSHERWOOD@PARTNERS.ORG] Sent: October 19, 2010 12:38 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette Marking The marking pencils work fine and we just ordered some marking pens from StatLab which work great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Tue Oct 19 16:23:46 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Oct 19 16:23:51 2010 Subject: [Histonet] Autofluorescence and literature for getting rid of the problem In-Reply-To: <006501cb6fc9$20059ee0$6010dca0$@callis@bresnan.net> References: <006501cb6fc9$20059ee0$6010dca0$@callis@bresnan.net> Message-ID: I would suggest using a sigma-aldrich product, Evans Blue (E2129). This product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988). Quick and very easy. William DeSalvo, B.S., HTL(ASCP) > From: gayle.callis@bresnan.net > To: histonet@lists.utsouthwestern.edu > Date: Tue, 19 Oct 2010 14:06:29 -0600 > Subject: [Histonet] Autofluorescence and literature for getting rid of the problem > > Richard, > > > > You will find what you need in a free pdf on autofluorescence from Wright > Imaging Facility in Toronto Canada. They have a website, then download > Autofluorescence: Causes and Cures. Also, if you can't get rid of > autofluorescence , use a near infrared fluorophore e.g. Alexa 750. There is > no autofluorescence seen in the NIR range. Just Google the title and it > will come up instantly. They also have a pdf on Mounting Media for > fluorescence work. > > > > > > For the little FFPE fluorescent work we do, you can try 100 to 300 mM > glycine in TRIS buffer pH 7.4 for 20 to 30 minutes before embarking on > immunostaining. Glycine binds free aldehydes to reduce the > autofluorescence but doesn't always work 100%. It may reduce the problem > but not totally eliminate it. > > > > There are other references on getting rid of autofluorescence, one for GFP, > a review, but it applies to FFPE tissue even not containing GFP. I will be > happy to send the pdf if you wish. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > Bozeman MT > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Tue Oct 19 16:26:23 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Oct 19 16:26:28 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <4CBE061A.7070702@pathology.washington.edu> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> <4CBE061A.7070702@pathology.washington.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5118@PHSXMB30.partners.org> Victor, We are a research lab and don't generate near the amount of specimens that a clinical lab would. We were happy to get an automatic stainer and coverslipper! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Tuesday, October 19, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette Marking I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: > Nita, > > We use HistoTec pens by Newcomer Supply > > ~Sean > > > On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > >> ** High Priority ** >> >> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? >> >> Anything else on the market? >> >> Thanks >> >> >> >> Nita Searcy, HT/HTL (ASCP) >> Scott and White Hospital >> Division Manager, Anatomic Pathology >> 2401 S. 31st. Street >> 254-724-2438 >> Temple, Texas, 76502 >> nsearcy@swmail.sw.org >> >> >> 254-724-2438 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From histotech <@t> imagesbyhopper.com Tue Oct 19 18:08:28 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Oct 19 18:08:54 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5118@PHSXMB30.partners.org> References: <4CBD9B8C.5D38.00EF.0@swmail.sw.org> <4CBE061A.7070702@pathology.washington.edu> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5118@PHSXMB30.partners.org> Message-ID: <2D6B56AD-4A9C-404D-81FA-44BA2E311249@imagesbyhopper.com> Statlab pens for us. We have a cassette printer, but we hand write our slides. I *love* the statlab pens!! Sent from my iPod On Oct 19, 2010, at 5:26 PM, "Sherwood, Margaret " wrote: > Victor, > > We are a research lab and don't generate near the amount of specimens that a > clinical lab would. We were happy to get an automatic stainer and coverslipper! > > Peggy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias > Sent: Tuesday, October 19, 2010 4:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Cassette Marking > > > I'm somewhat surprised that many labs are still handwriting blocks and > slides. If you are using a LIS, can it integrate printing blocks and > slides? Is the cost too high to add the printing capability? The cost of > equipment is so cheap compared with one lawsuit. > > It would also reduce the stress of loosing your job over a labeling > mistake. Just seems like a win win for everyone. > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > On 10/19/2010 1:38 PM, Sean McBride wrote: >> Nita, >> >> We use HistoTec pens by Newcomer Supply >> >> ~Sean >> >> >> On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: >> >>> ** High Priority ** >>> >>> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? > Pencils ? What is the rest of the world doing? >>> >>> Anything else on the market? >>> >>> Thanks >>> >>> >>> >>> Nita Searcy, HT/HTL (ASCP) >>> Scott and White Hospital >>> Division Manager, Anatomic Pathology >>> 2401 S. 31st. Street >>> 254-724-2438 >>> Temple, Texas, 76502 >>> nsearcy@swmail.sw.org >>> >>> >>> 254-724-2438 >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cbass <@t> wfubmc.edu Tue Oct 19 19:11:43 2010 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Tue Oct 19 19:12:37 2010 Subject: [Histonet] Coverslipping video Message-ID: Hello, I?m wondering if anyone could direct me to an online video of coverslipping, preferably permount. I?d like a reference to show some newbies in the lab. Thanks, Caroline From Susan.Walzer <@t> HCAHealthcare.com Wed Oct 20 02:19:14 2010 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Wed Oct 20 02:19:21 2010 Subject: [Histonet] RE: Plain Vanilla Autostainer? In-Reply-To: <59F7809368E0084C8AE6B38C93D735EB0924579D77@MEDEXMB02.ad.med.miami.edu> References: <59F7809368E0084C8AE6B38C93D735EB0924579D77@MEDEXMB02.ad.med.miami.edu> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2B3608C72A@FWDCWPMSGCMS09.hca.corpad.net> Leica Auto Stainer is the only way to go. It does all that you ask for and ours is old and still a work horse. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Tuesday, October 19, 2010 2:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Plain Vanilla Autostainer? I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Wed Oct 20 06:30:42 2010 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed Oct 20 06:30:57 2010 Subject: [Histonet] Cassette Marking In-Reply-To: <20101019140422.9e2d9aa830e8449a2412eb1e4f2f067e.54130242e4.wbe@email04.secureserver.net> References: <20101019140422.9e2d9aa830e8449a2412eb1e4f2f067e.54130242e4.wbe@email04.secureserver.net> Message-ID: <4CBE8C90.5D38.00EF.0@swmail.sw.org> As I sent to Victor- only "mission critical " items are being bought and as yet, I have not proved mission critical! One can;'t show errors not caught until way after the fact! Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> 10/19/2010 4:04 PM >>> As compared to a lawsuit, yes it's cheaper. But, when trying to explain to a budget committee that you need something to label things that you can do by hand...they usually don't see the point. This brings me back to my original point of mislabelling things 2 times in a year... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] Cassette Marking From: Victor Tobias Date: Tue, October 19, 2010 1:56 pm To: histonet@lists.utsouthwestern.edu I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: > Nita, > > We use HistoTec pens by Newcomer Supply > > ~Sean > > > On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > >> ** High Priority ** >> >> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? >> >> Anything else on the market? >> >> Thanks >> >> >> >> Nita Searcy, HT/HTL (ASCP) >> Scott and White Hospital >> Division Manager, Anatomic Pathology >> 2401 S. 31st. Street >> 254-724-2438 >> Temple, Texas, 76502 >> nsearcy@swmail.sw.org >> >> >> 254-724-2438 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From Kjones <@t> upei.ca Wed Oct 20 07:34:38 2010 From: Kjones <@t> upei.ca (Kathleen Jones) Date: Wed Oct 20 07:34:56 2010 Subject: [Histonet] m.bovis Message-ID: <4CBEB7AE0200008B0003522A@grpwise.novell.upei.ca> Hello Histonet! I know this has come up in the past, but I have not been able to find a solution in the archives...I am trying to get a mycoplasma bovis protocol worked up and am having tremendous difficulties. My antibody strain is M23 but it does not seem to be working very well for my IHC applications. I have tried several antigen retrieval methods, antibody dilutions and detection methods to no avail. I am thinking I need a new source of antibody...Can anyone suggest a commercially available m.bovis antibody that works for them? Any suggestions at all would be greatly appreciated! Thanks!! Kathleen Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)566-0595 From cmiller <@t> physlab.com Wed Oct 20 07:45:01 2010 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Oct 20 07:45:07 2010 Subject: [Histonet] RE: Plain Vanilla Autostainer? In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2B3608C72A@FWDCWPMSGCMS09.hca.corpad.net> References: <59F7809368E0084C8AE6B38C93D735EB0924579D77@MEDEXMB02.ad.med.miami.edu> <4BF03F5404EBDE409AF9232DA74B9DED2B3608C72A@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: I will second that! Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, October 20, 2010 2:19 AM To: KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? Leica Auto Stainer is the only way to go. It does all that you ask for and ours is old and still a work horse. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Tuesday, October 19, 2010 2:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Plain Vanilla Autostainer? I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From mhale <@t> carisls.com Wed Oct 20 08:01:40 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Wed Oct 20 08:01:45 2010 Subject: [Histonet] TN - HT Opprotunity Message-ID: <6F33D8418806044682A391273399860F05A28C39@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From MSHERWOOD <@t> PARTNERS.ORG Wed Oct 20 08:37:51 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Oct 20 08:38:01 2010 Subject: [Histonet] RE: Plain Vanilla Autostainer? In-Reply-To: References: <59F7809368E0084C8AE6B38C93D735EB0924579D77@MEDEXMB02.ad.med.miami.edu><4BF03F5404EBDE409AF9232DA74B9DED2B3608C72A@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB511C@PHSXMB30.partners.org> We also love our Leica Autostainer XL (refurbished). Kevin, that is the way to go if money is tight. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, October 20, 2010 8:45 AM To: Susan.Walzer@HCAHealthcare.com; KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? I will second that! Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, October 20, 2010 2:19 AM To: KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? Leica Auto Stainer is the only way to go. It does all that you ask for and ours is old and still a work horse. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Tuesday, October 19, 2010 2:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Plain Vanilla Autostainer? I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From b-frederick <@t> northwestern.edu Wed Oct 20 09:28:12 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Oct 20 09:28:37 2010 Subject: [Histonet] RE: Plain Vanilla Autostainer? In-Reply-To: Message-ID: <840407B459F6406BBD7FFF9C4C155DBB@lurie.northwestern.edu> We use the Autostainer XL as well. Just got a new one with the attached coverslipper. The old one is now in IHC for deparaffinization and running down before coverslipping. We have a program on the new autostainer that picks up from the load drawer and goes to the last xylene for the coverslipper to do its thing! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, October 20, 2010 7:45 AM To: Susan.Walzer@HCAHealthcare.com; KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? I will second that! Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, October 20, 2010 2:19 AM To: KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? Leica Auto Stainer is the only way to go. It does all that you ask for and ours is old and still a work horse. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Tuesday, October 19, 2010 2:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Plain Vanilla Autostainer? I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Wed Oct 20 09:48:14 2010 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Wed Oct 20 09:48:38 2010 Subject: [Histonet] Laser Capture Machine Message-ID: <4CBEBADE020000C500085471@mail.TSRH.ORG> To anybody using a laser capture machine, I want to ask your opinion on the following machine which one is better with regard to their instrumentation and cost of consumables because we are in the process of purchasing one and your honest opinion with regards to handling this machine is very much accountable. 1. Arcturus XT 2. Leica AS LMD 3. P.A.L.M LPC 4. MMI Cell Cut 5. Arcturus PixCell lle Thank you very much. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From relia1 <@t> earthlink.net Wed Oct 20 09:50:43 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Oct 20 09:50:55 2010 Subject: [Histonet] Histotech needed can you help? Message-ID: Hi Histonetters!! I hope you are enjoying a beautiful Fall Day. . I am currently working with a hospital in Northern Indiana about an hour south of Chicago that is in need of a histo tech. This is a permanent full time dayshift position. The client offers a great environment, a great crew to work with and excellent salary, benefits and relocation assistance. My question is do you know of anyone who might be interested in this position? If you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From alyssa <@t> alliedsearchpartners.com Wed Oct 20 10:03:06 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Oct 20 10:03:13 2010 Subject: [Histonet] Job Opening in San Diego Message-ID: Allied Search Partners is currently accepting resumes for a histotechnician/histotechnologist in San Diego, CA *Shift:* Full Time, Day Shift, Monday-Friday *Environment:* New State of the art Laboratory. Flexible hours. Ability to work side by side with Pathologist. No quotas. Family oriented environment. *Location:* San Diego, CA *Essential Functions and Duties:* Must be able to work independently under minimal supervision, maintain laboratory supplies, equipment, and QC/QA records The person should be reliable with great inter-personal communication skills, and willing to coordinate with Pathologist *Requirements:* Must meet the CLIA standards to gross** HT (ASCP) preferred. To apply for this position please submit resume to alyssa@alliedsearchpartners.com for initial prescreening. No resume will be submitted to client until we speak to you for a phone interview. All resumes kept confidential. -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From bcdukes <@t> lexhealth.org Wed Oct 20 10:08:05 2010 From: bcdukes <@t> lexhealth.org (Blake Taylor) Date: Wed Oct 20 10:08:12 2010 Subject: [Histonet] Job Opening in West Columbia, SC Message-ID: Hello everyone, we have an open position here at Lexington Medical Center in West Columbia, South Carolina for a full-time histotech. If you are interested or know anyone who may be please contact me or apply through our website. www.lexmed.com thanks Blake Taylor Section Supervisor Dept. of Pathology Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org ______________________________________________________________________ PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From drmtech09 <@t> gmail.com Wed Oct 20 11:10:18 2010 From: drmtech09 <@t> gmail.com (Eric Baltazar) Date: Wed Oct 20 11:10:23 2010 Subject: [Histonet] IEC-CTD & IEC Minotome Cryostat Wanted!!! Message-ID: Greetings to you all fellow Histonetters! Our company is currently looking to buy any available *Cryostat model IEC-CTD & Minotome *for use in our mobile Mohs laboratoy service business. If you still have these units in your offices and storages or probably know somebody who have this equipment and have not use it for years bec. they now have a much modern unit to use, contact us *ASAP* through this email add or on the phone listed below so we can coordinate purchasing and shipping procedure. We will take care of the shipping cost on these machines. This *Cryostat IEC-CTD* are the ones that looks like a mini-ref w/ caster wheels produced in the 70's,the Minotome would be the updated model w/ wider body and both are use to cut frozen sections. These are vintage machines but we can surely utilize them for our mobile Mohs lab business. TURN THAT SLEEPING MACHINE INTO *GREEN BUCKS* AND *WE WILL BUY THESE UNITS REGARDLESS OF THEIR CONDITIONS!!!!* So act and call us now! Sincerely, Eric T. Baltazar Dermtech Mohs Services Group Inc. Tel.no.:707-651-9420 From adam <@t> sensorhealth.com Wed Oct 20 11:52:36 2010 From: adam <@t> sensorhealth.com (Adam Harris) Date: Wed Oct 20 11:52:48 2010 Subject: [Histonet] RE: Cassette Marking Message-ID: Hi All, If anyone would like to try a free sample of the KP Lab marker for cassette, slide, or general purpose labeling, feel free to contact me and I will be more than happy to send you one. It's a win-win situation!! Adam Harris Sales Associate Sensor Health Inc. 110-6 Turnbull Crt. Cambridge, ON N1T 1K6 T: 1-888-777-7080 T: 519-621-1515 F: 519-621-8778 www.sensorhealth.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 83, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Cassette Marking (hymclab) 2. Autofluorescence and literature for getting rid of the problem (gayle callis) 3. Re: Cassette Marking (Sean McBride) 4. Re: Cassette Marking (Victor Tobias) 5. RE: Cassette Marking (sgoebel@xbiotech.com) 6. RE: Cassette Marking (Vanessa Avalos) 7. RE: Autofluorescence and literature for getting rid of the problem (WILLIAM DESALVO) 8. RE: Cassette Marking (Sherwood, Margaret ) 9. Re: Cassette Marking (histotech@imagesbyhopper.com) 10. Coverslipping video (Caroline Bass) 11. RE: Plain Vanilla Autostainer? (Susan.Walzer@HCAHealthcare.com) 12. RE: Cassette Marking (Nita Searcy) 13. m.bovis (Kathleen Jones) 14. RE: Plain Vanilla Autostainer? (Cheri Miller) 15. TN - HT Opprotunity (Hale, Meredith) 16. RE: RE: Plain Vanilla Autostainer? (Sherwood, Margaret ) 17. RE: RE: Plain Vanilla Autostainer? (Bernice Frederick) 18. Laser Capture Machine (Reuel Cornelia) 19. Histotech needed can you help? (Pam Barker) 20. Job Opening in San Diego (Alyssa Peterson) ---------------------------------------------------------------------- Message: 1 Date: Tue, 19 Oct 2010 14:49:18 -0500 From: hymclab Subject: RE: [Histonet] Cassette Marking To: "'Sherwood, Margaret '" , Nita Searcy , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We use the pens from Statlab also and love them. Dawn D. Schneider, HT(ASCP) Lead Histology Tech Howard Young Medical Center 240 Maple St. Woodruff, WI 54568 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, October 19, 2010 1:38 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette Marking The marking pencils work fine and we just ordered some marking pens from StatLab which work great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ------------------------------ Message: 2 Date: Tue, 19 Oct 2010 14:06:29 -0600 From: "gayle callis" Subject: [Histonet] Autofluorescence and literature for getting rid of the problem To: "Histonet" Message-ID: <006501cb6fc9$20059ee0$6010dca0$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" Richard, You will find what you need in a free pdf on autofluorescence from Wright Imaging Facility in Toronto Canada. They have a website, then download Autofluorescence: Causes and Cures. Also, if you can't get rid of autofluorescence , use a near infrared fluorophore e.g. Alexa 750. There is no autofluorescence seen in the NIR range. Just Google the title and it will come up instantly. They also have a pdf on Mounting Media for fluorescence work. For the little FFPE fluorescent work we do, you can try 100 to 300 mM glycine in TRIS buffer pH 7.4 for 20 to 30 minutes before embarking on immunostaining. Glycine binds free aldehydes to reduce the autofluorescence but doesn't always work 100%. It may reduce the problem but not totally eliminate it. There are other references on getting rid of autofluorescence, one for GFP, a review, but it applies to FFPE tissue even not containing GFP. I will be happy to send the pdf if you wish. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ------------------------------ Message: 3 Date: Tue, 19 Oct 2010 16:38:33 -0400 From: Sean McBride Subject: Re: [Histonet] Cassette Marking To: Nita Searcy Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Nita, We use HistoTec pens by Newcomer Supply ~Sean On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > ** High Priority ** > > If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? > > Anything else on the market? > > Thanks > > > > Nita Searcy, HT/HTL (ASCP) > Scott and White Hospital > Division Manager, Anatomic Pathology > 2401 S. 31st. Street > 254-724-2438 > Temple, Texas, 76502 > nsearcy@swmail.sw.org > > > 254-724-2438 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 19 Oct 2010 13:56:58 -0700 From: Victor Tobias Subject: Re: [Histonet] Cassette Marking To: histonet@lists.utsouthwestern.edu Message-ID: <4CBE061A.7070702@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: > Nita, > > We use HistoTec pens by Newcomer Supply > > ~Sean > > > On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > >> ** High Priority ** >> >> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? >> >> Anything else on the market? >> >> Thanks >> >> >> >> Nita Searcy, HT/HTL (ASCP) >> Scott and White Hospital >> Division Manager, Anatomic Pathology >> 2401 S. 31st. Street >> 254-724-2438 >> Temple, Texas, 76502 >> nsearcy@swmail.sw.org >> >> >> 254-724-2438 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 19 Oct 2010 14:04:22 -0700 From: Subject: RE: [Histonet] Cassette Marking To: "Victor Tobias" Cc: histonet@lists.utsouthwestern.edu Message-ID: <20101019140422.9e2d9aa830e8449a2412eb1e4f2f067e.54130242e4.wbe@email04.secu reserver.net> Content-Type: text/plain; charset="utf-8" As compared to a lawsuit, yes it's cheaper. But, when trying to explain to a budget committee that you need something to label things that you can do by hand...they usually don't see the point. This brings me back to my original point of mislabelling things 2 times in a year... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] Cassette Marking From: Victor Tobias Date: Tue, October 19, 2010 1:56 pm To: histonet@lists.utsouthwestern.edu I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: > Nita, > > We use HistoTec pens by Newcomer Supply > > ~Sean > > > On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > >> ** High Priority ** >> >> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? >> >> Anything else on the market? >> >> Thanks >> >> >> >> Nita Searcy, HT/HTL (ASCP) >> Scott and White Hospital >> Division Manager, Anatomic Pathology >> 2401 S. 31st. Street >> 254-724-2438 >> Temple, Texas, 76502 >> nsearcy@swmail.sw.org >> >> >> 254-724-2438 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 19 Oct 2010 13:26:01 -0700 From: "Vanessa Avalos" Subject: RE: [Histonet] Cassette Marking To: Message-ID: <000f01cb6fcb$dad7efa0$9087cee0$@com> Content-Type: text/plain; charset="us-ascii" Most places will also send you a sample. This way you can run test blocks in your processor to make sure you are happy w/ the results. Just remember to label the block w/ the pen name so you wont get confused! :) I currently use Mercedes Platinum Marker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqueline Farnsworth Sent: Tuesday, October 19, 2010 11:43 AM To: Sherwood, Margaret ; Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette Marking Surgipath 01880 Permanent Ink . Ultra fine point. Non-toxic Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret [MSHERWOOD@PARTNERS.ORG] Sent: October 19, 2010 12:38 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette Marking The marking pencils work fine and we just ordered some marking pens from StatLab which work great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 19 Oct 2010 15:23:46 -0600 From: WILLIAM DESALVO Subject: RE: [Histonet] Autofluorescence and literature for getting rid of the problem To: , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I would suggest using a sigma-aldrich product, Evans Blue (E2129). This product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988). Quick and very easy. William DeSalvo, B.S., HTL(ASCP) > From: gayle.callis@bresnan.net > To: histonet@lists.utsouthwestern.edu > Date: Tue, 19 Oct 2010 14:06:29 -0600 > Subject: [Histonet] Autofluorescence and literature for getting rid of the problem > > Richard, > > > > You will find what you need in a free pdf on autofluorescence from Wright > Imaging Facility in Toronto Canada. They have a website, then download > Autofluorescence: Causes and Cures. Also, if you can't get rid of > autofluorescence , use a near infrared fluorophore e.g. Alexa 750. There is > no autofluorescence seen in the NIR range. Just Google the title and it > will come up instantly. They also have a pdf on Mounting Media for > fluorescence work. > > > > > > For the little FFPE fluorescent work we do, you can try 100 to 300 mM > glycine in TRIS buffer pH 7.4 for 20 to 30 minutes before embarking on > immunostaining. Glycine binds free aldehydes to reduce the > autofluorescence but doesn't always work 100%. It may reduce the problem > but not totally eliminate it. > > > > There are other references on getting rid of autofluorescence, one for GFP, > a review, but it applies to FFPE tissue even not containing GFP. I will be > happy to send the pdf if you wish. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > Bozeman MT > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 19 Oct 2010 17:26:23 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] Cassette Marking To: "Victor Tobias" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5118@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" Victor, We are a research lab and don't generate near the amount of specimens that a clinical lab would. We were happy to get an automatic stainer and coverslipper! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Tuesday, October 19, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette Marking I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: > Nita, > > We use HistoTec pens by Newcomer Supply > > ~Sean > > > On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > >> ** High Priority ** >> >> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? >> >> Anything else on the market? >> >> Thanks >> >> >> >> Nita Searcy, HT/HTL (ASCP) >> Scott and White Hospital >> Division Manager, Anatomic Pathology >> 2401 S. 31st. Street >> 254-724-2438 >> Temple, Texas, 76502 >> nsearcy@swmail.sw.org >> >> >> 254-724-2438 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 9 Date: Tue, 19 Oct 2010 19:08:28 -0400 From: "histotech@imagesbyhopper.com" Subject: Re: [Histonet] Cassette Marking To: "Sherwood, Margaret" Cc: "" Message-ID: <2D6B56AD-4A9C-404D-81FA-44BA2E311249@imagesbyhopper.com> Content-Type: text/plain; charset=us-ascii Statlab pens for us. We have a cassette printer, but we hand write our slides. I *love* the statlab pens!! Sent from my iPod On Oct 19, 2010, at 5:26 PM, "Sherwood, Margaret " wrote: > Victor, > > We are a research lab and don't generate near the amount of specimens that a > clinical lab would. We were happy to get an automatic stainer and coverslipper! > > Peggy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias > Sent: Tuesday, October 19, 2010 4:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Cassette Marking > > > I'm somewhat surprised that many labs are still handwriting blocks and > slides. If you are using a LIS, can it integrate printing blocks and > slides? Is the cost too high to add the printing capability? The cost of > equipment is so cheap compared with one lawsuit. > > It would also reduce the stress of loosing your job over a labeling > mistake. Just seems like a win win for everyone. > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > On 10/19/2010 1:38 PM, Sean McBride wrote: >> Nita, >> >> We use HistoTec pens by Newcomer Supply >> >> ~Sean >> >> >> On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: >> >>> ** High Priority ** >>> >>> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? > Pencils ? What is the rest of the world doing? >>> >>> Anything else on the market? >>> >>> Thanks >>> >>> >>> >>> Nita Searcy, HT/HTL (ASCP) >>> Scott and White Hospital >>> Division Manager, Anatomic Pathology >>> 2401 S. 31st. Street >>> 254-724-2438 >>> Temple, Texas, 76502 >>> nsearcy@swmail.sw.org >>> >>> >>> 254-724-2438 >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Tue, 19 Oct 2010 20:11:43 -0400 From: Caroline Bass Subject: [Histonet] Coverslipping video To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Hello, I9m wondering if anyone could direct me to an online video of coverslipping, preferably permount. I9d like a reference to show some newbies in the lab. Thanks, Caroline ------------------------------ Message: 11 Date: Wed, 20 Oct 2010 02:19:14 -0500 From: Subject: [Histonet] RE: Plain Vanilla Autostainer? To: , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2B3608C72A@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Leica Auto Stainer is the only way to go. It does all that you ask for and ours is old and still a work horse. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Tuesday, October 19, 2010 2:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Plain Vanilla Autostainer? I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 20 Oct 2010 06:30:42 -0500 From: "Nita Searcy" Subject: RE: [Histonet] Cassette Marking To: "Victor Tobias" , Cc: histonet@lists.utsouthwestern.edu Message-ID: <4CBE8C90.5D38.00EF.0@swmail.sw.org> Content-Type: text/plain; charset="us-ascii" As I sent to Victor- only "mission critical " items are being bought and as yet, I have not proved mission critical! One can;'t show errors not caught until way after the fact! Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 >>> 10/19/2010 4:04 PM >>> As compared to a lawsuit, yes it's cheaper. But, when trying to explain to a budget committee that you need something to label things that you can do by hand...they usually don't see the point. This brings me back to my original point of mislabelling things 2 times in a year... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: Re: [Histonet] Cassette Marking From: Victor Tobias Date: Tue, October 19, 2010 1:56 pm To: histonet@lists.utsouthwestern.edu I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: > Nita, > > We use HistoTec pens by Newcomer Supply > > ~Sean > > > On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: > >> ** High Priority ** >> >> If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? >> >> Anything else on the market? >> >> Thanks >> >> >> >> Nita Searcy, HT/HTL (ASCP) >> Scott and White Hospital >> Division Manager, Anatomic Pathology >> 2401 S. 31st. Street >> 254-724-2438 >> Temple, Texas, 76502 >> nsearcy@swmail.sw.org >> >> >> 254-724-2438 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ------------------------------ Message: 13 Date: Wed, 20 Oct 2010 09:34:38 -0300 From: "Kathleen Jones" Subject: [Histonet] m.bovis To: Message-ID: <4CBEB7AE0200008B0003522A@grpwise.novell.upei.ca> Content-Type: text/plain; charset=US-ASCII Hello Histonet! I know this has come up in the past, but I have not been able to find a solution in the archives...I am trying to get a mycoplasma bovis protocol worked up and am having tremendous difficulties. My antibody strain is M23 but it does not seem to be working very well for my IHC applications. I have tried several antigen retrieval methods, antibody dilutions and detection methods to no avail. I am thinking I need a new source of antibody...Can anyone suggest a commercially available m.bovis antibody that works for them? Any suggestions at all would be greatly appreciated! Thanks!! Kathleen Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)566-0595 ------------------------------ Message: 14 Date: Wed, 20 Oct 2010 07:45:01 -0500 From: Cheri Miller Subject: [Histonet] RE: Plain Vanilla Autostainer? To: "Susan.Walzer@HCAHealthcare.com" , "KJohnson@med.miami.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I will second that! Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, October 20, 2010 2:19 AM To: KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? Leica Auto Stainer is the only way to go. It does all that you ask for and ours is old and still a work horse. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Tuesday, October 19, 2010 2:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Plain Vanilla Autostainer? I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 15 Date: Wed, 20 Oct 2010 08:01:40 -0500 From: "Hale, Meredith" Subject: [Histonet] TN - HT Opprotunity To: Message-ID: <6F33D8418806044682A391273399860F05A28C39@s-irv-ex301.PathologyPartners.intr anet> Content-Type: text/plain; charset="us-ascii" Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com ------------------------------ Message: 16 Date: Wed, 20 Oct 2010 09:37:51 -0400 From: "Sherwood, Margaret " Subject: RE: [Histonet] RE: Plain Vanilla Autostainer? To: "Cheri Miller" , , , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB511C@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" We also love our Leica Autostainer XL (refurbished). Kevin, that is the way to go if money is tight. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, October 20, 2010 8:45 AM To: Susan.Walzer@HCAHealthcare.com; KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? I will second that! Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, October 20, 2010 2:19 AM To: KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? Leica Auto Stainer is the only way to go. It does all that you ask for and ours is old and still a work horse. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Tuesday, October 19, 2010 2:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Plain Vanilla Autostainer? I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 17 Date: Wed, 20 Oct 2010 09:28:12 -0500 From: "Bernice Frederick" Subject: RE: [Histonet] RE: Plain Vanilla Autostainer? To: "'Cheri Miller'" , , , Message-ID: <840407B459F6406BBD7FFF9C4C155DBB@lurie.northwestern.edu> Content-Type: text/plain; charset="us-ascii" We use the Autostainer XL as well. Just got a new one with the attached coverslipper. The old one is now in IHC for deparaffinization and running down before coverslipping. We have a program on the new autostainer that picks up from the load drawer and goes to the last xylene for the coverslipper to do its thing! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, October 20, 2010 7:45 AM To: Susan.Walzer@HCAHealthcare.com; KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? I will second that! Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, October 20, 2010 2:19 AM To: KJohnson@med.miami.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plain Vanilla Autostainer? Leica Auto Stainer is the only way to go. It does all that you ask for and ours is old and still a work horse. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Tuesday, October 19, 2010 2:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Plain Vanilla Autostainer? I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization --> H&E; (3) H&E only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30" X 33" X 34" vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 20 Oct 2010 09:48:14 -0500 From: "Reuel Cornelia" Subject: [Histonet] Laser Capture Machine To: Message-ID: <4CBEBADE020000C500085471@mail.TSRH.ORG> Content-Type: text/plain; charset=US-ASCII To anybody using a laser capture machine, I want to ask your opinion on the following machine which one is better with regard to their instrumentation and cost of consumables because we are in the process of purchasing one and your honest opinion with regards to handling this machine is very much accountable. 1. Arcturus XT 2. Leica AS LMD 3. P.A.L.M LPC 4. MMI Cell Cut 5. Arcturus PixCell lle Thank you very much. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ------------------------------ Message: 19 Date: Wed, 20 Oct 2010 10:50:43 -0400 From: "Pam Barker" Subject: [Histonet] Histotech needed can you help? To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! I hope you are enjoying a beautiful Fall Day. . I am currently working with a hospital in Northern Indiana about an hour south of Chicago that is in need of a histo tech. This is a permanent full time dayshift position. The client offers a great environment, a great crew to work with and excellent salary, benefits and relocation assistance. My question is do you know of anyone who might be interested in this position? If you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 20 Date: Wed, 20 Oct 2010 11:03:06 -0400 From: Alyssa Peterson Subject: [Histonet] Job Opening in San Diego To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Allied Search Partners is currently accepting resumes for a histotechnician/histotechnologist in San Diego, CA *Shift:* Full Time, Day Shift, Monday-Friday *Environment:* New State of the art Laboratory. Flexible hours. Ability to work side by side with Pathologist. No quotas. Family oriented environment. *Location:* San Diego, CA *Essential Functions and Duties:* Must be able to work independently under minimal supervision, maintain laboratory supplies, equipment, and QC/QA records The person should be reliable with great inter-personal communication skills, and willing to coordinate with Pathologist *Requirements:* Must meet the CLIA standards to gross** HT (ASCP) preferred. To apply for this position please submit resume to alyssa@alliedsearchpartners.com for initial prescreening. No resume will be submitted to client until we speak to you for a phone interview. All resumes kept confidential. -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 83, Issue 33 **************************************** From Sara.Phinney <@t> leica-microsystems.com Wed Oct 20 13:24:43 2010 From: Sara.Phinney <@t> leica-microsystems.com (Sara.Phinney@leica-microsystems.com) Date: Wed Oct 20 13:24:51 2010 Subject: [Histonet] Sara Phinney-availability Message-ID: I will be out of the office starting 10/20/2010 and will not return until 10/22/2010. I will be in the office but with limited availability from October18th till Friday October 22. I will be checking voicemail and email periodically throughout the day. My best, Sara Please contact Mark Burton in my absence: mark.burton@leica-microsystems.Com If you require technical assistance please dial 1-800-248-0123. For all other matters please call Timm Piper at 617-653-2605. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From kenneth.a.troutman <@t> Vanderbilt.Edu Wed Oct 20 13:44:47 2010 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Wed Oct 20 13:46:13 2010 Subject: [Histonet] IHC techs needed Message-ID: <7B310892042DA74CB3590053F424CFE61381DA02AD@ITS-HCWNEM06.ds.Vanderbilt.edu> Vanderbilt University is seeking two certified techs for 2nd shift. Primary duties will be IHC staining. Anyone interested please visit www.mc.vanderbilt.edu and click on "Careers at Vanderbilt". Select "Current staff openings" and type "Histotech" in the keyword search. Thank you! Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 From sbruce <@t> vetpathservicesinc.com Wed Oct 20 15:52:26 2010 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Wed Oct 20 15:55:09 2010 Subject: [Histonet] Celestine Blue for Technovit 7200 Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A20104546E@vpss1.VetPathServicesInc.local> Hi everyone, I'm searching for a procedure or help w/a stain for thick ground sections using Technovit 7200. The stain in question is a celestine blue/alum hematoxylin w/a Van Gieson counterstain. Anyone have a procedure or recommendations? Thanks in advance, Suzanne sbruce@vetpathservicesinc.com From sgoebel <@t> xbiotech.com Wed Oct 20 15:55:22 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Wed Oct 20 15:55:27 2010 Subject: [Histonet] Human anti human Message-ID: <20101020135522.9e2d9aa830e8449a2412eb1e4f2f067e.191adcd28c.wbe@email04.secureserver.net> Hey ya'll I was going through the archives to try and find a solution to this, but the last post was in 2008, so I thought I would throw it back out there. I need a human anti human polymer...is there one yet? I looked and couldn't find one through my normal places. As to not start the conversation that is on the archive up again. I have tried several approaches already to no avail. I tried FITC, I conjugated the primary human anti human with HRP, I've tried TSA (and tons of other companies version of TSA)...no luck... My antibody just isn't staining...I even tried putting it on the tissue and PBMCs at a 1:1 dilution...nope...any thoughts out there? I want to rip my hair out Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 From ratliffjack <@t> hotmail.com Wed Oct 20 16:04:21 2010 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Oct 20 16:04:27 2010 Subject: [Histonet] Celestine Blue for Technovit 7200 In-Reply-To: <26DB1FDFBF9EE14AB3AACC873519A4A20104546E@vpss1.VetPathServicesInc.local> References: <26DB1FDFBF9EE14AB3AACC873519A4A20104546E@vpss1.VetPathServicesInc.local> Message-ID: Why not use Sanderson's Rapid Bone Stain with Van Gieson picrofuchsin? You can get the Sanderson's and one or all of three counterstains for this particular kind of staining in a kit from Dorn and Hart Microedge (www.dornandhart.com). Jack > Date: Wed, 20 Oct 2010 16:52:26 -0400 > From: sbruce@vetpathservicesinc.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Celestine Blue for Technovit 7200 > > Hi everyone, > > I'm searching for a procedure or help w/a stain for thick ground sections using Technovit 7200. The stain in question is a celestine blue/alum hematoxylin w/a Van Gieson counterstain. Anyone have a procedure or recommendations? > > Thanks in advance, > Suzanne > sbruce@vetpathservicesinc.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aaperghis <@t> uspath.com Wed Oct 20 18:04:22 2010 From: aaperghis <@t> uspath.com (Adrienne Aperghis Kavanagh) Date: Wed Oct 20 18:04:26 2010 Subject: [Histonet] QMS MANUAL In-Reply-To: <6388062.379090.1287615661530.JavaMail.root@mail3d.brinkster.com> Message-ID: <7180861.379098.1287615862813.JavaMail.root@mail3d.brinkster.com> Does anyone have a basic format or table of contents they would be willing to share regarding a QMS (Quality Management Systems) Manual? I am in the process of getting one together and am a little overwhelmed. We are supposed to base our QMS on NYSDOH...as if that helps. Even a link in the right direction would be MUCH appreciated. You can message me privately, if you wish. Thanks in advance, friends! ADRIENNE From sbruce <@t> vetpathservicesinc.com Wed Oct 20 18:49:31 2010 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Wed Oct 20 18:51:43 2010 Subject: [Histonet] Celestine Blue for Technovit 7200 References: <26DB1FDFBF9EE14AB3AACC873519A4A20104546E@vpss1.VetPathServicesInc.local> Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A201045477@vpss1.VetPathServicesInc.local> Thanks Jack, what's the difference between the celestine blue w/alum hematoxylin and the Sanderson's? What's the difference between the Sanderson's and the Masson's Goldner's? Have you tried the celestine blue stain? Thanks in advance, Suzanne ________________________________ From: Jack Ratliff [mailto:ratliffjack@hotmail.com] Sent: Wed 10/20/2010 5:04 PM To: Suzanne Bruce; Histonet Subject: RE: [Histonet] Celestine Blue for Technovit 7200 Why not use Sanderson's Rapid Bone Stain with Van Gieson picrofuchsin? You can get the Sanderson's and one or all of three counterstains for this particular kind of staining in a kit from Dorn and Hart Microedge (www.dornandhart.com ). Jack > Date: Wed, 20 Oct 2010 16:52:26 -0400 > From: sbruce@vetpathservicesinc.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Celestine Blue for Technovit 7200 > > Hi everyone, > > I'm searching for a procedure or help w/a stain for thick ground sections using Technovit 7200. The stain in question is a celestine blue/alum hematoxylin w/a Van Gieson counterstain. Anyone have a procedure or recommendations? > > Thanks in advance, > Suzanne > sbruce@vetpathservicesinc.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Thu Oct 21 07:57:23 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Oct 21 07:57:31 2010 Subject: [Histonet] Histology Supervisor Opening in the Southeast Message-ID: Allied Search Partners has been retained in the search for a Histology Supervisor for a Baton Rouge, LA client. *Position Title: *Histology Supervisor *Lab Environment: *Private Practice, State of the art Equipment, Family Oriented Envronment *Job Description: *The is a ?working supervisor? position so this individual with have a little bit of bench work on a daily basis including routine histology, special staining, and IHC. *Shifts:* 2am-10am, Monday-Friday, Permanent/Long Term Benefits: Fully benefited with Medical Insurance, Paid Time Off, Relocation Assistance, and more to be discussed with the HR manager if phone interview is requested. *Requirements: * At least 2-5 years experience in a lead/supervisory role and HTL or HT ASCP certified *To apply:* - Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com - Please also email some availability over the course of the next few days for a phone screen with one of our recruiters. Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for a generous bonus, and have your resume reviewed for free by our career advisors. -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From Kim.Donadio <@t> bhcpns.org Thu Oct 21 09:21:19 2010 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Thu Oct 21 09:21:27 2010 Subject: [Histonet] Histotechnologist position in Pensacola Fl Message-ID: Histological Technologist Job Code: 200410300405 Baptist Hospital, Pensacola, FL Dept: Pathology (4103) - Full Time - 4 10hr shifts or 5/8 hr days - Day Shift SUMMARY OF MAJOR FUNCTIONS: The Histological Technologist is a clinical laboratory technologist with a specialty licensure in histology who works with in the pathology department to prepare samples for evaluation by the pathologist using specialized knowledge and skills. This individual may direct and guide the activities of ancillary personnel regarding pathology samples while maintaining the standards of Baptist Health Care. The person in this position works under general supervision, is responsible for various shifts, may be subject to over 40 hours per week and/or callback as required, and may also be required to remain on campus immediately before, during and after severe weather and/or disasters. QUALIFICATIONS FOR JOB: ? AS degree in Histology perferred ? Current Florida clinical laboratory license in the technologist level ? HT(ASCP) preferred ? 1 year experience preferred Salary and Benefits are competitive Shifts are flexible Please contact me directly or go to our Baptist web site and fill out a application. http://www.ebaptisthealthcare.org/Careers/ Have a great week everyone! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From JWatson <@t> gnf.org Thu Oct 21 10:10:27 2010 From: JWatson <@t> gnf.org (James Watson) Date: Thu Oct 21 10:10:42 2010 Subject: [Histonet] test Message-ID: Testing other messages have not been posted James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From JWeems <@t> sjha.org Thu Oct 21 12:26:24 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Oct 21 12:26:29 2010 Subject: [Histonet] New CPT codes Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640448AF218F@CHEXCMS10.one.ads.che.org> Do ya'll use the new codes, 88387 and 88388, when you collect tissue for research purposes? Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Cathy.Crumpton <@t> tuality.org Thu Oct 21 12:33:20 2010 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Thu Oct 21 12:33:26 2010 Subject: [Histonet] CAP question ANP.22970 Message-ID: How is everyone handling this new question about Annual Result Co mparison for ER and PR? The question states "...the laboratory at lea benchmarks, and eva pathologists in the laboratory.. program or do this by hand? < Cathy Crumpton HT(ASCP), Histology Lead Tuality Hillsboro, OR 97123 (503)681-1292 From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Oct 21 12:56:50 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Oct 21 12:57:48 2010 Subject: [Histonet] CAP question ANP.22970 In-Reply-To: References: Message-ID: We do this by hand. Usually it involves a summer student to compile all of the data on a spread sheet and a formula to compute the percentage of positive cases. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org [Cathy.Crumpton@tuality.org] Sent: Thursday, October 21, 2010 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question ANP.22970 How is everyone handling this new question about Annual Result Co mparison for ER and PR? The question states "...the laboratory at lea= annually compares its patient results with published benchmarks, and eva=ates interobserver variability among the pathologists in the laboratory..= Do you have a separate computer program or do this by hand? <=V> Cathy Crumpton HT(ASCP), Histology Lead Tuality=mmunity Hospital Hillsboro, OR 97123 (503)681-1292 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Thu Oct 21 13:07:26 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Oct 21 13:10:26 2010 Subject: [Histonet] CAP question ANP.22970 In-Reply-To: References: Message-ID: <61135F0455D33347B5AAE209B903A30433DEBEBE@EXCHVS2.medctr.ad.wfubmc.edu> We are doing it by hand as well, into a spread sheet, and then doing the calculations. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, October 21, 2010 1:57 PM To: Cathy.Crumpton@tuality.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question ANP.22970 We do this by hand. Usually it involves a summer student to compile all of the data on a spread sheet and a formula to compute the percentage of positive cases. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org [Cathy.Crumpton@tuality.org] Sent: Thursday, October 21, 2010 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question ANP.22970 How is everyone handling this new question about Annual Result Co mparison for ER and PR? The question states "...the laboratory at lea= annually compares its patient results with published benchmarks, and eva=ates interobserver variability among the pathologists in the laboratory..= Do you have a separate computer program or do this by hand? <=V> Cathy Crumpton HT(ASCP), Histology Lead Tuality=mmunity Hospital Hillsboro, OR 97123 (503)681-1292 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Oct 21 13:33:19 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Oct 21 13:33:35 2010 Subject: [Histonet] interfacing the IHC bond and Cassette labelers to Co-Path Message-ID: <047F1D56-97D8-4A6D-BC0A-1E8F599BE4A3@yahoo.com> Hi out there in Histo Land! I would like your assistance in answering a question that was proposed by a friend who is not a histonet member. I don't have the answer, but know that one of you would. Below is the question: Could you help me justify the importance of interfacing our IHC bond and Cassette labelers to Co-Path? A simple paragraph, or if you have, a white paper, that would be great. I am attempting to get the interfaces approved through our IT Department and running up against some roadblocks. Thank you in advance for your assistance, Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From silvinamolinuevo <@t> yahoo.com.ar Thu Oct 21 13:47:19 2010 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Thu Oct 21 13:59:36 2010 Subject: [Histonet] Resorption pits Message-ID: <594308.8855.qm@web113608.mail.gq1.yahoo.com> Hi!! I am working with dentina slice to study resorption pits. I am not very sure how to analyze the results. Has anyone experience and can help me? thank you very much! silvina molinuevo From mollie <@t> phylogenyinc.com Thu Oct 21 14:02:39 2010 From: mollie <@t> phylogenyinc.com (Mollie Hannon) Date: Thu Oct 21 14:03:28 2010 Subject: [Histonet] Histotechnologist position in Columbus, Ohio Message-ID: <1D0FA32F-EFC0-426F-9574-87EE0B0A6B3B@phylogenyinc.com> Small Columbus-based biotech company is searching for an experienced, fun loving histotech. The ideal candidate will be a personable, able to adjust quickly to fluctuating priorities and the responsibilities that come with helping a company grow. We?d like more than just your average ?histotech.? We?re looking for a unique individual who has keen business sense, project management experience, excellent coomunication skills & the ability to effectively and compassionately work with others both inside and outside of the office. Essentially, we give you the ball and you run with a "whatever it takes" attitude. Skills & responsibilities should include, but are not limited to: -Routine cutting and embedding tissue -Staining Slides -Lab management -Project management This is a full time position, but part-time will be considered for the right candidate. Please send resume and references to mollie@phylogenyinc.com. Competitive compensation with full benefits. From mward <@t> wfubmc.edu Thu Oct 21 14:07:17 2010 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Oct 21 14:07:45 2010 Subject: [Histonet] paperless requisitions Message-ID: <61135F0455D33347B5AAE209B903A30433DEBEC3@EXCHVS2.medctr.ad.wfubmc.edu> I am posting this question for our AP manager and supervisor. Our anatomic pathology lab is starting to market their services, along with our clinical laboratories. Our LIS would like us to move toward paperless surgical pathology requisitions, to more closely mimic some of the larger outside reference labs. Is anyone out there currently paperless? We would be interested in seeing how you accomplished it, any pitfalls, examples of your on-line req, etc. Thanks in advance for any responses. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 From akemiat3377 <@t> yahoo.com Thu Oct 21 15:01:23 2010 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Oct 21 15:01:43 2010 Subject: [Histonet] Clarification-interfacing the IHC bond and Cassette labelers to Co-Path In-Reply-To: <0B8979A204680A42B93A52B486088CD9030489DD8F@CUAEXH1.GCU-MD.local> References: <047F1D56-97D8-4A6D-BC0A-1E8F599BE4A3@yahoo.com> <0B8979A204680A42B93A52B486088CD9030489DD8F@CUAEXH1.GCU-MD.local> Message-ID: <58CEE549-91D2-40DD-8554-9C071B5D783A@yahoo.com> Hi Walter and Histo-subscribers, Ist I want to thank Walter for his quick reply. I appreciate your answer! 2nd, I appreciate any and all replies, but does anyone have an "article" that addresses issues that can occur such as: Efficiency Omitting Duplication of Tests ordered: Additional Slides, Special Stains, IHC, FISH, CISH, Cost effectiveness due to omission of errors Patient Safety Thanks Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Oct 21, 2010, at 11:38 AM, Walter Benton wrote: > Efficiency > Patient Safety > Orders for the Bond come directly from the LIS and can not be > misunderstood due to poor handwriting, since they are interfaced > with the LIS. > > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 126 > (All Deliveries to Suite 127) > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > wbenton@cua.md > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison > [akemiat3377@yahoo.com] > Sent: Thursday, October 21, 2010 2:33 PM > To: Histonet > Subject: [Histonet] interfacing the IHC bond and Cassette labelers > to Co-Path > > Hi out there in Histo Land! > > I would like your assistance in answering a question that was > proposed by a friend who is not a histonet member. I don't have the > answer, but know that one of you would. Below is the question: > > Could you help me justify the importance of interfacing our IHC bond > and Cassette labelers to Co-Path? A simple paragraph, or if you > have, a white paper, that would be great. I am attempting to get the > interfaces approved through our IT Department and running up against > some roadblocks. > > Thank you in advance for your assistance, > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Thu Oct 21 15:33:28 2010 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Oct 21 15:33:31 2010 Subject: [Histonet] Eosin to dye small Biopsies Message-ID: <1872B4A455B7974391609AD8034C79FC026DFCFD@LBEXCH01.hchd.local> Hello to all in histoland. Are any of you using eosin on the processor to dye your small bx's? If so, are you putting it in the 100% alcohol to do so? Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From kimd <@t> slonepartners.com Thu Oct 21 15:34:37 2010 From: kimd <@t> slonepartners.com (Kim Wilson) Date: Thu Oct 21 15:34:42 2010 Subject: [Histonet] Looking for a new opportunity in the DC area? Message-ID: Hi, I'm looking to fill a Histotech, Pathology Assistant, and HLA Lead position in the D.C./Baltimore area. My client has an excellent benefits package and offers the ability to be a part of an innovative, growing laboratory. Please give me a call or send me an email if you'd like more information. Thanks! Kim Wilson, Executive Recruiter with Slone Partners - 877-436-8105 or email kimd@slonepartners.com SLONEPARTNERS KIM WILSON - EXECUTIVE RECRUITER FORT WORTH, TX Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877-436-8105 DIRECT: 817-595-2227 KIMD@SLONEPARTNERS.COM SLONEPARTNERS.COM The information contained is privileged and confidential information owned by Slone Partners and is only for the individual or entry named above. If you receive this in error please discard-do not transmit further. From HornHV <@t> archildrens.org Thu Oct 21 15:39:31 2010 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Oct 21 15:40:00 2010 Subject: [Histonet] RE: Eosin to dye small Biopsies In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFCFD@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFCFD@LBEXCH01.hchd.local> Message-ID: <25A4DE08332B19499904459F00AAACB7181249D0A0@EVS1.archildrens.org> We put it in our 95% Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, October 21, 2010 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin to dye small Biopsies Hello to all in histoland. Are any of you using eosin on the processor to dye your small bx's? If so, are you putting it in the 100% alcohol to do so? Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From histotech <@t> imagesbyhopper.com Thu Oct 21 15:39:38 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Oct 21 15:40:06 2010 Subject: [Histonet] Eosin to dye small Biopsies In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFCFD@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFCFD@LBEXCH01.hchd.local> Message-ID: <1BCE9617-D148-4EA6-9A11-3E886226FF3E@imagesbyhopper.com> We use about 40ml of eosin in the first 100% alcohol in both of our "large specimen" & "small biopsy" machines. Michelle On Oct 21, 2010, at 4:33 PM, "Scott, Allison D" wrote: > Hello to all in histoland. Are any of you using eosin on the processor > to dye your small bx's? If so, are you putting it in the 100% alcohol > to do so? Any help in this matter will be greatly appreciated. > > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jbaez <@t> interscopepath.com Thu Oct 21 15:40:04 2010 From: jbaez <@t> interscopepath.com (Baez, Janet) Date: Thu Oct 21 15:43:55 2010 Subject: [Histonet] Eosin to dye small Biopsies Message-ID: <9E956D8FEB06C2408B08AC16498325E9255ECD@scopemx1.interscope.com> We put the eosin in the last 100% alcohol on the processor for all specimens. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, October 21, 2010 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin to dye small Biopsies Hello to all in histoland. Are any of you using eosin on the processor to dye your small bx's? If so, are you putting it in the 100% alcohol to do so? Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmorales <@t> adrian.edu Thu Oct 21 16:22:09 2010 From: mmorales <@t> adrian.edu (Marti Morales) Date: Thu Oct 21 16:22:14 2010 Subject: [Histonet] Leica / Reichert-Jung 2030 Biocut Microtome Message-ID: <4CC0AF01.7060608@adrian.edu> Does anyone use a Leica / Reichert-Jung 2030 Biocut Microtome? I am looking for some information on this piece of equipment. If anyone can help, I would greatly appreciate it! Kind regards, Marti Morales-Ensign From Traczyk7 <@t> aol.com Thu Oct 21 16:49:52 2010 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Thu Oct 21 16:50:07 2010 Subject: [Histonet] Looking for Jodelle Sparks Message-ID: <1aceec.7034dddb.39f20f80@aol.com> Jodelle if you're reading this, please let me know how to get a hold of you now. If anyone knows how to reach her would you please pass this request along to her for me. Thanks, Dorothy From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 21 16:52:25 2010 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Thu Oct 21 16:52:34 2010 Subject: [Histonet] interfacing the IHC bond and Cassette labelers to Co-Path In-Reply-To: <047F1D56-97D8-4A6D-BC0A-1E8F599BE4A3@yahoo.com> References: <047F1D56-97D8-4A6D-BC0A-1E8F599BE4A3@yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4C0303F309B6@EXMBMCB15.ucsfmedicalcenter.org> Akemi, here are a couple papers on barcoding in the AP lab: Reengineered Workflow in the Anatomic Pathology Laboratory: Costs and Benefits. Archives of pathology & laboratory medicine (2009) volume: 133 issue: 4 page: 601 The Henry Ford Production System: reduction of surgical pathology in-process misidentification defects by bar code-specified work process standardization. Am J Clin Pathol. 2009 Apr;131(4):468-77. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Thursday, October 21, 2010 11:33 AM To: Histonet Subject: [Histonet] interfacing the IHC bond and Cassette labelers to Co-Path Hi out there in Histo Land! I would like your assistance in answering a question that was proposed by a friend who is not a histonet member. I don't have the answer, but know that one of you would. Below is the question: Could you help me justify the importance of interfacing our IHC bond and Cassette labelers to Co-Path? A simple paragraph, or if you have, a white paper, that would be great. I am attempting to get the interfaces approved through our IT Department and running up against some roadblocks. Thank you in advance for your assistance, Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Thu Oct 21 21:45:17 2010 From: jclark <@t> pcnm.com (Joanne Clark) Date: Thu Oct 21 21:45:23 2010 Subject: [Histonet] Grossing Station Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C0139AB04@mail.pcnm.com> Does anyone have a used grossing station sitting around in storage that they would like to get rid of? Joanne Clark, HT Histo Supervisor Pathology Consultants of New Mexico From Susan.Walzer <@t> HCAHealthcare.com Fri Oct 22 02:28:37 2010 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Oct 22 02:28:53 2010 Subject: [Histonet] Eosin to dye small Biopsies In-Reply-To: <1BCE9617-D148-4EA6-9A11-3E886226FF3E@imagesbyhopper.com> References: <1872B4A455B7974391609AD8034C79FC026DFCFD@LBEXCH01.hchd.local> <1BCE9617-D148-4EA6-9A11-3E886226FF3E@imagesbyhopper.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2B361EBD88@FWDCWPMSGCMS09.hca.corpad.net> We use about the same amount but in our last 100% Alc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, October 21, 2010 4:40 PM To: Scott, Allison D Cc: Subject: Re: [Histonet] Eosin to dye small Biopsies We use about 40ml of eosin in the first 100% alcohol in both of our "large specimen" & "small biopsy" machines. Michelle On Oct 21, 2010, at 4:33 PM, "Scott, Allison D" wrote: > Hello to all in histoland. Are any of you using eosin on the processor > to dye your small bx's? If so, are you putting it in the 100% alcohol > to do so? Any help in this matter will be greatly appreciated. > > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alina211 <@t> student.liu.se Fri Oct 22 05:29:05 2010 From: alina211 <@t> student.liu.se (Ali Nasr Esfahani) Date: Fri Oct 22 05:29:11 2010 Subject: [Histonet] Endothelial histochemistry Message-ID: Hi, I am just new in this group, so I hope this is the way I can get help. I am doing histochemistry on human hypothalamus and I need to stain endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as the whole and like a line. But I would like to have an image of the endothelial cells of even larger vessels, the way that I can show cell by cell in a circular shape of a vessel. Could you help me and tell me what antibody or lectin helps me best. Regards Ali From godsgalnow <@t> aol.com Fri Oct 22 06:06:33 2010 From: godsgalnow <@t> aol.com (=?utf-8?B?Z29kc2dhbG5vd0Bhb2wuY29t?=) Date: Fri Oct 22 06:37:35 2010 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gRW9zaW4gdG8gZHllIHNtYWxsIEJpb3BzaWVz?= Message-ID: <201010221106.o9MB6FMs008416@imr-da05.mx.aol.com> We use cobalt blue as it does not interfere with FISH Sent from my Verizon Wireless Phone ----- Reply message ----- From: Susan.Walzer@HCAHealthcare.com Date: Fri, Oct 22, 2010 3:28 am Subject: [Histonet] Eosin to dye small Biopsies To: , Cc: We use about the same amount but in our last 100% Alc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, October 21, 2010 4:40 PM To: Scott, Allison D Cc: Subject: Re: [Histonet] Eosin to dye small Biopsies We use about 40ml of eosin in the first 100% alcohol in both of our "large specimen" & "small biopsy" machines. Michelle On Oct 21, 2010, at 4:33 PM, "Scott, Allison D" wrote: > Hello to all in histoland. Are any of you using eosin on the processor > to dye your small bx's? If so, are you putting it in the 100% alcohol > to do so? Any help in this matter will be greatly appreciated. > > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Fri Oct 22 06:55:42 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Oct 22 06:55:56 2010 Subject: [Histonet] Eosin to dye small Biopsies In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFCFD@LBEXCH01.hchd.local> Message-ID: We use eosin at the grossing bench on endoscopic specimens and hematoxylin on prostate/liver biopsies. The pathologist drops a drop of stain on each specimen. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Scott, Allison D" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/21/2010 04:37 PM To cc Subject [Histonet] Eosin to dye small Biopsies Hello to all in histoland. Are any of you using eosin on the processor to dye your small bx's? If so, are you putting it in the 100% alcohol to do so? Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From k84as <@t> yahoo.com Fri Oct 22 07:36:15 2010 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Oct 22 07:36:20 2010 Subject: [Histonet] pituitary stain Message-ID: <817566.83408.qm@web112620.mail.gq1.yahoo.com> --- dear all can you share me pituitary stain protocol called PAS/ celestine blue / Orange G? ? thanks in advance ? mohamed From Ronald.Houston <@t> nationwidechildrens.org Fri Oct 22 09:23:38 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Oct 22 09:23:49 2010 Subject: [Histonet] pituitary stain In-Reply-To: <817566.83408.qm@web112620.mail.gq1.yahoo.com> References: <817566.83408.qm@web112620.mail.gq1.yahoo.com> Message-ID: Mohamed, The University of Nottingham Histology department has an extensive website of histology protocols: http://www.nottingham.ac.uk/pathology/default.html There is a protocol for PAS/OG on that site. I do not have any experience using it, but as it comes out of John Bancroft's old lab it will undoubtedly be very sound. It does differ somewhat from the method of AGE Pearse that I followed many, many moons ago in Glasgow. We also always performed an OFG (orange-fuchsin-green) method on all pituitary bxs (if you need a copy of that let me know).As I'm sure you are aware, tinctorial stains for pituitary adenomas have been replaced with immunohistochemistry for the hormones ACTH, FSH, GH, LH, prolactin and TSH: PAS/OG 1. Section to 70% alcohol 2. leave in 70% alcohol for 5 minutes 3. transfer to Solution "A" for 15 minutes at room temp 4. Wash in 70% alcohol for 5 minutes 5. transfer to reducing rinse "B" for 5 minutes 6. 70% alcohol for 3 minutes 7. rinse in running tap water 8. Schiff's reagent 20-30 minutes 9. wash in water 3 minutes 10. Celestin Blue solution 10 minutes 11. Rinse well in water 12. Hematoxylin 10 minutes 13. wash in water 14. 2% Orange G in 5% phosphotungstic acid for 3 minutes 15. wash in water 16. dehydrate, clear and mount Result Nuclei - black RBC's - yellow-orange Alpha granules - orange Beta granules - magenta (PAS +ve) Solution "A" Periodic acid - 400mg Distilled water - 15 ml 100% alcohol - 35 ml Reducing Solution "B" Sodium thiosulphate - 1gm Distilled water - 20ml 100% alcohol - 30ml HCl - 0.5ml Rationale PAS converts 1,2 glycols to aldehyde. The reducing step is thought to block the subsequent reaction of collagen and retic fibers with the Schiff's reagent. Beta granules contain muco- or glycoproteins that react with Schiff's to give their magenta staining. Orange G, which is an acidic dye, stains the alpha granules. As RBC's are also acidophilic in nature, they also pick up the orange coloration. Reference Pearse AGE. The cytochemical demonstration of gonadotrophic hormone in the human anterior hypophysis. J Pathol Bacteriol. 1949: 61; 195-202 If you need a recipe for the celestin blue solution, drop me a line and I'll get it to you, but you should find it in most good histotechnology text-books, or on-line Best wishes Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, October 22, 2010 8:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] pituitary stain --- dear all can you share me pituitary stain protocol called PAS/ celestine blue / Orange G? thanks in advance mohamed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From neelyk <@t> shands.ufl.edu Fri Oct 22 09:31:31 2010 From: neelyk <@t> shands.ufl.edu (Kendall Neely) Date: Fri Oct 22 09:31:42 2010 Subject: [Histonet] Need opinions on H&E stainers please! Message-ID: <4CC16808.3024.0059.1@shands.ufl.edu> Our laboratory will be purchasing new H&E stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 From rsrichmond <@t> gmail.com Fri Oct 22 09:41:00 2010 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Oct 22 09:41:05 2010 Subject: [Histonet] Re: Eosin to dye small Biopsies Message-ID: Allison Scott at LBJ Hospital in Houston, Texas asks about the use of eosin to dye small biopsy specimens. Several replies mention addition of eosin to one of the processing alcohols. I have never seen this done, in maybe 60 pathology services I've worked in. (I'd know, because I nearly always examine the paraffin block when I order recuts or send a case out for consultation.) It's a fine time-waster for the pathologist to mark small specimens with dye while grossing. I've used Mercurochrome (merbromin, related to eosin but with 26% mercury) which fortunately was banned in the USA about ten years ago. I've used eosin, and I've used safranin (from the microbiology lab's Gram stain setup). I don't know whether safranin interferes with FISH, as eosin is well known to, nor do I know if you can put safranin in the processing alcohol. And I've used Davidson tissue marking inks. I've never seen or heard of cobalt blue used for this purpose - is this the insoluble coloring material, chemically cobalt aluminate? Bob Richmond Samurai Pathologist Knoxville TN From Bill.Tench <@t> pph.org Fri Oct 22 09:51:37 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Oct 22 09:51:43 2010 Subject: [Histonet] eosin Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5751@MAIL1.pph.local> I think the use in a processor becomes a local culture. I think everyone in my community does it (someone spread the word), so now we all do. I believe that we are all putting it in the last alcohol. It really makes facing a block on minute pieces a whole lot easier. We found marking the tissue directly tedious, and it didn't persist as well in the processing. Because there is often so little visible material in our FNA cell blocks, we mark the histogel pellet with Davidson ink, and when the tech has just faced off the ink, he/she knows its time to start collecting sections. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From klauing <@t> lumc.edu Fri Oct 22 10:01:56 2010 From: klauing <@t> lumc.edu (Kristen Lauing) Date: Fri Oct 22 10:02:26 2010 Subject: [Histonet] Endothelial histochemistry Message-ID: <4CC16114020000FD000204FA@gwgwia2.luhs.org> Hi Ali, Although I do not ever perform endothelial immunohistochemistry in our laboratory, I know it is common to use an antibody against CD31 to selectively stain endothelial cells and this may work well in pituitary tissue. Hope that helps, Kristen >>> Ali Nasr Esfahani 10/22/10 5:31 AM >>> Hi, I am just new in this group, so I hope this is the way I can get help. I am doing histochemistry on human hypothalamus and I need to stain endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as the whole and like a line. But I would like to have an image of the endothelial cells of even larger vessels, the way that I can show cell by cell in a circular shape of a vessel. Could you help me and tell me what antibody or lectin helps me best. Regards Ali _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Fri Oct 22 10:10:54 2010 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Oct 22 10:11:06 2010 Subject: [Histonet] Re: Eosin to dye small Biopsies In-Reply-To: Message-ID: As much as I respect Dr. Richmond, I would have to disagree that staining bx's with eosin is a waste of pathologist time. It helps the embedding tech and cutting tech see the minute pieces, which may be otherwise lost. Sometimes that is the diagnostic material. We would not want to put a patient through another procedure because we couldn't recover the tissue submitted. We use a vial with a dropper. Once the biopsy is placed in the cassette you take 1 second more to drop a drop of eosin on the specimen. Well worth everyone's time in my humble opinion. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Robert Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 10/22/2010 10:44 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Eosin to dye small Biopsies Allison Scott at LBJ Hospital in Houston, Texas asks about the use of eosin to dye small biopsy specimens. Several replies mention addition of eosin to one of the processing alcohols. I have never seen this done, in maybe 60 pathology services I've worked in. (I'd know, because I nearly always examine the paraffin block when I order recuts or send a case out for consultation.) It's a fine time-waster for the pathologist to mark small specimens with dye while grossing. I've used Mercurochrome (merbromin, related to eosin but with 26% mercury) which fortunately was banned in the USA about ten years ago. I've used eosin, and I've used safranin (from the microbiology lab's Gram stain setup). I don't know whether safranin interferes with FISH, as eosin is well known to, nor do I know if you can put safranin in the processing alcohol. And I've used Davidson tissue marking inks. I've never seen or heard of cobalt blue used for this purpose - is this the insoluble coloring material, chemically cobalt aluminate? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From sgoebel <@t> xbiotech.com Fri Oct 22 10:15:08 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 22 10:15:12 2010 Subject: [Histonet] Need opinions on H&E stainers please! Message-ID: <20101022081508.9e2d9aa830e8449a2412eb1e4f2f067e.5c0f7dbef8.wbe@email04.secureserver.net> For the love of your sanity (and keeping your pathologists happy) NOT BUY A SYMPHONY!!!! Their reagents are all proprietary and so troubleshooting is almost impossible without paying Ventana to have tech he before y (depending on yo before you can file. trying this thing out hated t consistent from slide to slide. N the thing and the reagents are insane ex Just my opinion =) Sarah Goebel, B.A., H Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 < Austin, Te (512)386-2907 -------- Original Message -------- Subject: [Histonet] Need opinions on H&E stainers please! From: "Kendall Neely" <[1]neelyk@s Date: Fri, October 22, 2010 7:31 am To: <[2]histonet@lists Our laboratory will be purchasing new H&E stain equipment in the near f Thermo-Sh Ventana Symphony. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 _______________________________________________ Histonet mailing list [3]Histonet@lists.utsouth [4]http: References 1. 3D"mailto:neelyk@shands.ufl.edu" 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From histotech <@t> imagesbyhopper.com Fri Oct 22 10:17:31 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Oct 22 10:17:59 2010 Subject: [Histonet] eosin In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5751@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A5751@MAIL1.pph.local> Message-ID: <1F4763A3-EFC6-49A0-A77A-31146427AC54@imagesbyhopper.com> It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol! I agree with Bill, it really makes a HUGE difference in small, minute tissues. We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes! We have since switched to an aqua color and no more hiding specimens! ;o) Michelle On Oct 22, 2010, at 10:51 AM, "Tench, Bill" wrote: > I think the use in a processor becomes a local culture. I think > everyone in my community does it (someone spread the word), so now we > all do. I believe that we are all putting it in the last alcohol. It > really makes facing a block on minute pieces a whole lot easier. We > found marking the tissue directly tedious, and it didn't persist as well > in the processing. > Because there is often so little visible material in our FNA cell > blocks, we mark the histogel pellet with Davidson ink, and when the tech > has just faced off the ink, he/she knows its time to start collecting > sections. > > Bill Tench > Associate Dir. Laboratory Services > Chief, Cytology Services > Palomar Medical Center > 555 E. Valley Parkway > Escondido, California 92025 > Bill.Tench@pph.org > Voice: 760- 739-3037 > Fax: 760-739-2604 > > > [None] made the following annotations > --------------------------------------------------------------------- > Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lblazek <@t> digestivespecialists.com Fri Oct 22 10:22:51 2010 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Oct 22 10:22:57 2010 Subject: [Histonet] eosin In-Reply-To: <1F4763A3-EFC6-49A0-A77A-31146427AC54@imagesbyhopper.com> References: <2820431BF953BB4DA3E9E1A5882265FD034A5751@MAIL1.pph.local> <1F4763A3-EFC6-49A0-A77A-31146427AC54@imagesbyhopper.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EB213402D@IBMB7Exchange.digestivespecialists.com> We put eosin in the first 100% alcohol. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, October 22, 2010 11:18 AM To: Tench, Bill Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] eosin It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol! I agree with Bill, it really makes a HUGE difference in small, minute tissues. We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes! We have since switched to an aqua color and no more hiding specimens! ;o) Michelle On Oct 22, 2010, at 10:51 AM, "Tench, Bill" wrote: > I think the use in a processor becomes a local culture. I think > everyone in my community does it (someone spread the word), so now we > all do. I believe that we are all putting it in the last alcohol. It > really makes facing a block on minute pieces a whole lot easier. We > found marking the tissue directly tedious, and it didn't persist as well > in the processing. > Because there is often so little visible material in our FNA cell > blocks, we mark the histogel pellet with Davidson ink, and when the tech > has just faced off the ink, he/she knows its time to start collecting > sections. > > Bill Tench > Associate Dir. Laboratory Services > Chief, Cytology Services > Palomar Medical Center > 555 E. Valley Parkway > Escondido, California 92025 > Bill.Tench@pph.org > Voice: 760- 739-3037 > Fax: 760-739-2604 > > > [None] made the following annotations > --------------------------------------------------------------------- > Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Oct 22 10:44:50 2010 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Oct 22 10:44:44 2010 Subject: [Histonet] Need opinions on H&E stainers please! In-Reply-To: <4CC16808.3024.0059.1@shands.ufl.edu> References: <4CC16808.3024.0059.1@shands.ufl.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5146@PHSXMB30.partners.org> We have a (refurbished) Lecia Autostainer XL and really like it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kendall Neely Sent: Friday, October 22, 2010 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need opinions on H&E stainers please! Our laboratory will be purchasing new H&E stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From cfitz <@t> 007group.com Fri Oct 22 10:46:33 2010 From: cfitz <@t> 007group.com (Cathy) Date: Fri Oct 22 10:46:37 2010 Subject: [Histonet] eosin In-Reply-To: <1F4763A3-EFC6-49A0-A77A-31146427AC54@imagesbyhopper.com> References: <2820431BF953BB4DA3E9E1A5882265FD034A5751@MAIL1.pph.local> <1F4763A3-EFC6-49A0-A77A-31146427AC54@imagesbyhopper.com> Message-ID: <1C6133BCDE0C4CD088C3E580362285E5@your8ba846406f> We add 1.0 mls of 10% aqueous Eosin to the first 100% Ethanol; we will then have Eosin in all of the Ethanol containers after they have been rotated. This removed the time consuming task of dotting each tissue at grossing and allows for easy visualization at embedding and sectioning. I have not had a complaint about the eosin interfering with FISH from our referral lab. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital _____ From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, October 22, 2010 8:18 AM To: Tench, Bill Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] eosin It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol! I agree with Bill, it really makes a HUGE difference in small, minute tissues. We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes! We have since switched to an aqua color and no more hiding specimens! ;o) Michelle On Oct 22, 2010, at 10:51 AM, "Tench, Bill" wrote: > I think the use in a processor becomes a local culture. I think > everyone in my community does it (someone spread the word), so now we > all do. I believe that we are all putting it in the last alcohol. It > really makes facing a block on minute pieces a whole lot easier. We > found marking the tissue directly tedious, and it didn't persist as well > in the processing. > Because there is often so little visible material in our FNA cell > blocks, we mark the histogel pellet with Davidson ink, and when the tech > has just faced off the ink, he/she knows its time to start collecting > sections. > > Bill Tench > Associate Dir. Laboratory Services > Chief, Cytology Services > Palomar Medical Center > 555 E. Valley Parkway > Escondido, California 92025 > Bill.Tench@pph.org > Voice: 760- 739-3037 > Fax: 760-739-2604 > > > [None] made the following annotations > --------------------------------------------------------------------- > Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1136 / Virus Database: 422/3212 - Release Date: 10/22/10 From mucram11 <@t> comcast.net Fri Oct 22 10:51:26 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Oct 22 10:51:30 2010 Subject: [Histonet] Need opinions on H&E stainers please! In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5146@PHSXMB30.partners.org> Message-ID: <476612518.114420.1287762686096.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We have two leica XL Stainers and love them.? We don't have the unit to transfer to the coverslippers yet and that would be great but minor overall.? We are just setting up some special stains on it and the run up- run down programs for special stains.? Pam Marcum UAMS ----- Original Message ----- From: "Margaret Sherwood" To: "Kendall Neely" , histonet@lists.utsouthwestern.edu Sent: Friday, October 22, 2010 10:44:50 AM Subject: RE: [Histonet] Need opinions on H&E stainers please! We have a (refurbished) Lecia Autostainer XL and really like it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kendall Neely Sent: Friday, October 22, 2010 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need opinions on H&E stainers please! Our laboratory will be purchasing new H&E stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Margaret Sherwood" To: "Kendall Neely" , histonet@lists.utsouthwestern.edu Sent: Friday, October 22, 2010 10:44:50 AM Subject: RE: [Histonet] Need opinions on H&E stainers please! We have a (refurbished) Lecia Autostainer XL and really like it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kendall Neely Sent: Friday, October 22, 2010 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need opinions on H&E stainers please! Our laboratory will be purchasing new H&E stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfitz <@t> 007group.com Fri Oct 22 10:52:51 2010 From: cfitz <@t> 007group.com (Cathy) Date: Fri Oct 22 10:52:55 2010 Subject: [Histonet] Need opinions on H&E stainers please! In-Reply-To: <4CC16808.3024.0059.1@shands.ufl.edu> References: <4CC16808.3024.0059.1@shands.ufl.edu> Message-ID: <94AAF15B1C414D8D9BCAE5E46325E743@your8ba846406f> We are using the Sakura Prisma with attached coverslipper; it is a workhorse and allows for the use of what ever reagents/stains that you prefer. We are running our H&E and H Pylori stains simultaneously. We experienced a small problem with the leveling of the instrument at the initial set up but once that was corrected it has been working well since then. The only draw back that I can see with the attached coverslipper is that the slides take longer to dry than with the older model. The slides are kept horizontal rather than vertical so the xylene doesn't run off. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital _____ From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kendall Neely Sent: Friday, October 22, 2010 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need opinions on H&E stainers please! Our laboratory will be purchasing new H&E stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1136 / Virus Database: 422/3212 - Release Date: 10/22/10 From a.thotakura <@t> imperial.ac.uk Fri Oct 22 10:57:46 2010 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Fri Oct 22 10:58:29 2010 Subject: [Histonet] Endothelial histochemistry In-Reply-To: Message-ID: Hi Ali, I tried FITC cd31 on mouse liver frozen section with tumors it worked really well. Good luck, Anil Kumar. On 22/10/10 11:29, "Ali Nasr Esfahani" wrote: Hi, I am just new in this group, so I hope this is the way I can get help. I am doing histochemistry on human hypothalamus and I need to stain endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as the whole and like a line. But I would like to have an image of the endothelial cells of even larger vessels, the way that I can show cell by cell in a circular shape of a vessel. Could you help me and tell me what antibody or lectin helps me best. Regards Ali _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kcruise <@t> path.wustl.edu Fri Oct 22 11:16:55 2010 From: kcruise <@t> path.wustl.edu (Cruise, Karen) Date: Fri Oct 22 11:17:34 2010 Subject: [Histonet] Processors Message-ID: <6428E3A48152704F838E99641B0973FA2EBFA7@PATHEXCH.wusm-path.wustl.edu> Greetings Histologist, Does anyone have positive or negative comments on the Thermo Citadel 1000 ? Is there an abundance of fumes during processing? Thanks again, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcruise@path.wustl.edu From ahacking <@t> yahoo.com Fri Oct 22 11:42:52 2010 From: ahacking <@t> yahoo.com (Adam Hacking) Date: Fri Oct 22 11:42:56 2010 Subject: [Histonet] Microtome for undecalcified tissues Message-ID: <650431.99045.qm@web30804.mail.mud.yahoo.com> Hi, ? I'm looking for a good used sledge or rotary microtome capable of cutting MMA emebdded bone specimens. Can anyone reccomend a good model, manufacturer, supplier or know of a good used machine ? ? Many thanks. ? Adam From alyssa <@t> alliedsearchpartners.com Fri Oct 22 11:45:29 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Oct 22 11:45:54 2010 Subject: [Histonet] Histo Opening in MA Message-ID: MPath Search Partners is looking for a Histotechnician/Histotechnologist candidate for permanent direct hire in Massachusetts. *Shift/Schedule:* Monday-Friday, Day Shift Hours * * *Position: *Histotechnician/Histotechnologist *Location: * Pharmaceutical Company in the Burlington, MA area *Requirements:* HT/HTL ASCP preferred Degree preferred Must have experience with working with Animal Tissue *Benefits:* Generous PTO accrual schedule 401k Competitive wages/benefits Paid training Health Benefits *Application:* To be considered please submit your resume in order to schedule a phone screen with one of our recruiters. Please be aware that all resumes submitted to Allied Search Partners are confidential. *Serious inquiries only please.* 1. No resume will be submitted until we speak to you for a phone screen. 2. No resume will be submitted to client if you are currently or previously worked for client. 3. Name, and contact information will not be given out without your consent. Please submit resume to Alyssa@alliedsearchpartners.com -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From mucram11 <@t> comcast.net Fri Oct 22 11:49:31 2010 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Oct 22 11:49:33 2010 Subject: [Histonet] Microtome for undecalcified tissues In-Reply-To: <650431.99045.qm@web30804.mail.mud.yahoo.com> Message-ID: <513444361.116148.1287766171353.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Depending on the size of the bone the Leica 2255 is a great mictorome to use with tungsten carbide blades.? It is heavy enough to larger blocks and is motorized.? If it is not motorized I would not try it for as that will give you the best results. Pam Marcum ----- Original Message ----- From: "Adam Hacking" To: Histonet@lists.utsouthwestern.edu Sent: Friday, October 22, 2010 11:42:52 AM Subject: [Histonet] Microtome for undecalcified tissues Hi, ? I'm looking for a good used sledge or rotary microtome capable of cutting MMA emebdded bone specimens. Can anyone reccomend a good model, manufacturer, supplier or know of a good used machine ? ? Many thanks. ? Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- Original Message ----- From: "Adam Hacking" To: Histonet@lists.utsouthwestern.edu Sent: Friday, October 22, 2010 11:42:52 AM Subject: [Histonet] Microtome for undecalcified tissues Hi, ? I'm looking for a good used sledge or rotary microtome capable of cutting MMA emebdded bone specimens. Can anyone reccomend a good model, manufacturer, supplier or know of a good used machine ? ? Many thanks. ? Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Fri Oct 22 12:00:15 2010 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Oct 22 12:00:35 2010 Subject: [Histonet] I Message-ID: I will be out of the office starting 10/21/2010 and will not return until 10/25/2010. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. From alyssa <@t> alliedsearchpartners.com Fri Oct 22 12:04:30 2010 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Oct 22 12:04:35 2010 Subject: [Histonet] Histotech Opening in the Midwest Message-ID: MPath Search Partners is looking for a Histotechncian/Histotechnologist candidate for permanent direct hire in Wisconsin. *Shift/Schedule:* Monday-Friday, Day Shift Hours, 38 hours per week * * *Position: *Histotechnician/Histotechnologist *Location: * State of the art laboratory in Manitowoc, Wisconsin 10 Dermatology offices throughout WI One centralized physician office laboratory *Requirements:* HT/HTL ASCP preferred Degree preferred Mohs experience *Benefits:* * Generous PTO accrual schedule * 401k match & profit sharing * Competitive wages/benefits * Paid holidays * Paid training *Application:* To be considered please submit your resume in order to schedule a phone screen with one of our recruiters. Please be aware that all resumes submitted to Allied Search Partners are confidential. *Serious inquiries only please.* 1. No resume will be submitted until we speak to you for a phone screen. 2. No resume will be submitted to client if you are currently or previously worked for client. 3. Name, and contact information will not be given out without your consent. Please submit resume to Alyssa@alliedsearchpartners.com -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From sfeher <@t> CMC-NH.ORG Fri Oct 22 12:12:32 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Oct 22 12:12:35 2010 Subject: [Histonet] QMS MANUAL In-Reply-To: <7180861.379098.1287615862813.JavaMail.root@mail3d.brinkster.com> References: <6388062.379090.1287615661530.JavaMail.root@mail3d.brinkster.com> <7180861.379098.1287615862813.JavaMail.root@mail3d.brinkster.com> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2426C73D@exchange.cmc-nh.org> I would recommend a CAP publication, "Quality Management in Anatomic Pathology; Promoting Patient Safety Through Systems Improvement and Error Reduction", Raouf E. Nakhleh, MD, Patrick L. Fitzgibbons, MD, Editors. You should be able to order it on the CAP website. It's not very expensive and does a good job of outlining a great QM Manual. There are lots of sample pages that can be readily adapted to your particular lab. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Aperghis Kavanagh Sent: Wednesday, October 20, 2010 7:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QMS MANUAL Does anyone have a basic format or table of contents they would be willing to share regarding a QMS (Quality Management Systems) Manual? I am in the process of getting one together and am a little overwhelmed. We are supposed to base our QMS on NYSDOH...as if that helps. Even a link in the right direction would be MUCH appreciated. You can message me privately, if you wish. Thanks in advance, friends! ADRIENNE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Fri Oct 22 12:31:14 2010 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Oct 22 12:31:20 2010 Subject: [Histonet] Re: Eosin to dye small Biopsies In-Reply-To: References: Message-ID: We have our grossing staff cover all GI biopsies, breast biopsies and endometrial curettings or any small colorless tissue with 0.1% basic fuchsin. It leaves it a nice pinkish tint after processing, the specimens are visible in the paraffin, and we haven't had any pathologist complaints. On Fri, Oct 22, 2010 at 8:10 AM, wrote: > As much as I respect Dr. Richmond, I would have to disagree that staining > bx's with eosin is a waste of pathologist time. It helps the embedding > tech and cutting tech see the minute pieces, which may be otherwise lost. > Sometimes that is the diagnostic material. > We would not want to put a patient through another procedure because we > couldn't recover the tissue submitted. > We use a vial with a dropper. Once the biopsy is placed in the cassette > you take 1 second more to drop a drop of eosin on the specimen. > Well worth everyone's time in my humble opinion. > > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkboyd@chs.net > > > > > > > > Robert Richmond > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/22/2010 10:44 AM > > To > histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] Re: Eosin to dye small Biopsies > > > > > > > Allison Scott at LBJ Hospital in Houston, Texas asks about the use of > eosin to dye small biopsy specimens. > > Several replies mention addition of eosin to one of the processing > alcohols. I have never seen this done, in maybe 60 pathology services > I've worked in. (I'd know, because I nearly always examine the > paraffin block when I order recuts or send a case out for > consultation.) > > It's a fine time-waster for the pathologist to mark small specimens > with dye while grossing. I've used Mercurochrome (merbromin, related > to eosin but with 26% mercury) which fortunately was banned in the USA > about ten years ago. I've used eosin, and I've used safranin (from the > microbiology lab's Gram stain setup). I don't know whether safranin > interferes with FISH, as eosin is well known to, nor do I know if you > can put safranin in the processing alcohol. And I've used Davidson > tissue marking inks. > > I've never seen or heard of cobalt blue used for this purpose - is > this the insoluble coloring material, chemically cobalt aluminate? > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From sfeher <@t> CMC-NH.ORG Fri Oct 22 12:32:07 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Oct 22 12:32:11 2010 Subject: [Histonet] Clarification-interfacing the IHC bond and Cassettelabelers to Co-Path In-Reply-To: <58CEE549-91D2-40DD-8554-9C071B5D783A@yahoo.com> References: <047F1D56-97D8-4A6D-BC0A-1E8F599BE4A3@yahoo.com><0B8979A204680A42B93A52B486088CD9030489DD8F@CUAEXH1.GCU-MD.local> <58CEE549-91D2-40DD-8554-9C071B5D783A@yahoo.com> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2426C73E@exchange.cmc-nh.org> We have interfaced our Bonds with Soft Path LIS system. My justification for this started with using it for LEAN processes in that the orders for IHC went directly from the Pathologist to the Bond and eliminated the need for my techs to have to input individual orders for IHC by hand. Since we have set up our slide labelers to be recognized as just another printer as far as the LIS is concerned, we do not use paper labels at all but have 2d barcodes printed directly on our slides. When an order is put in by the pathologist for IHC, my techs can see the order, cut the section and print the slide with the correct bar code. Bond recognizes the barcode and initializes the tests that have been ordered and transferred from the pathologist. This has accomplished the following: No tech time lost in printing labels for slides to go on the bond. No ambiguity or lost IHC orders due to hand writing orders by the pathologist. No chance of keystroke errors on the part of my IHC tech while putting manual orders into the Bond. In addition to eliminating hand writing and manual keystrokes, which are distinct patient safety issue, I have calculated that having the interface has saved me approximately 0.7 FTE. Instead of having to hire extra staff to cover increased workloads or wasting existing staff on extraneous tasks (hand labeling, manually entering orders, etc), I can utilize them in other areas. The patient safety aspect of eliminating extra tasks involving manual data entry is huge. A majority of the lawsuits against pathology labs involve some aspect of human error resulting from manual tasks in labeling or data entry. In addition to being able to market my lab as patient safety focused, we have eliminated a major source of potential lawsuits. It's hard to put a price tag on what that saves other than to say that the costs are sometimes much more than the dollar figures paid out. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Thursday, October 21, 2010 4:01 PM To: Walter Benton Cc: Histonet Subject: Re: [Histonet] Clarification-interfacing the IHC bond and Cassettelabelers to Co-Path Hi Walter and Histo-subscribers, Ist I want to thank Walter for his quick reply. I appreciate your answer! 2nd, I appreciate any and all replies, but does anyone have an "article" that addresses issues that can occur such as: Efficiency Omitting Duplication of Tests ordered: Additional Slides, Special Stains, IHC, FISH, CISH, Cost effectiveness due to omission of errors Patient Safety Thanks Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Oct 21, 2010, at 11:38 AM, Walter Benton wrote: > Efficiency > Patient Safety > Orders for the Bond come directly from the LIS and can not be > misunderstood due to poor handwriting, since they are interfaced with > the LIS. > > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 126 > (All Deliveries to Suite 127) > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > wbenton@cua.md > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison > [akemiat3377@yahoo.com] > Sent: Thursday, October 21, 2010 2:33 PM > To: Histonet > Subject: [Histonet] interfacing the IHC bond and Cassette labelers > to Co-Path > > Hi out there in Histo Land! > > I would like your assistance in answering a question that was > proposed by a friend who is not a histonet member. I don't have the > answer, but know that one of you would. Below is the question: > > Could you help me justify the importance of interfacing our IHC bond > and Cassette labelers to Co-Path? A simple paragraph, or if you > have, a white paper, that would be great. I am attempting to get the > interfaces approved through our IT Department and running up against > some roadblocks. > > Thank you in advance for your assistance, > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri Oct 22 12:43:52 2010 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Oct 22 12:44:00 2010 Subject: [Histonet] formalin specimen containers Message-ID: Does anyone know of or have access to changes in regulations for formalin specimen containers? At our recent CAP inspection, we were told that JCAHO and OSHA were mandating that formalin specimen containers should now have "locking" lids. This is the first I have heard of this. Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From cbass <@t> wfubmc.edu Fri Oct 22 12:50:09 2010 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Fri Oct 22 12:50:32 2010 Subject: [Histonet] Cheap slide scanner for rat brains? Message-ID: Hello Everyone, Does anyone have a good way to get low magnification images from slides? I have some DAB stained rat coronal sections that I would like to digitize. Basically, I want to have a picture of the entire section. It doesn?t have to be high resolution, just enough to make out the basic structures. Can someone recommend a way to do this? I?ve used a flat bed scanner in the past, and I know some folks use an actual film slide scanner. What I?m interested in are tips for finding a solution, particularly what to avoid, and model numbers that people have used. I don?t want to keep buying scanners until I found one that works. And obviously I?d like to keep the expenses to a minimum. Finally, has anyone tried this scanner? It?s a little pricey for me, but I do like the idea behind it, although I wonder if it?s just one of their stock scanners that they have repainted, added an adapter and jacked up the price for. It looks identical to other scanners that they sell for $300. http://www.meyerinst.com/html/oem/pse4/ Thanks, Caroline From trathborne <@t> somerset-healthcare.com Fri Oct 22 12:56:30 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Oct 22 12:57:08 2010 Subject: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC2426C73E@exchange.cmc-nh.org> Message-ID: I'd be interested in knowing how this has impacted your pathologists. Were they resistant at first? The extra time inputting requests would now fall on them. And there is still a chance of keystroke errors with pathologist entry. I'm not disagreeing with the comments you made, only wondering since you were able to save .7 FTE, where the .7 pathologist came from. Or is it the interface itself that saves the time, and not who enters it? Also, did you have the slide printers before the LEAN process was implemented? or was it done because of LEAN and is part of the FTE savings? I'm very interested in this process, but know the types of questions I'll need to have answers for. We're a Cerner facility, so some things will be different but the principal will be the same. Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Feher, Stephen Sent: Friday, October 22, 2010 1:32 PM To: Akemi Allison; Walter Benton Cc: Histonet Subject: RE: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path We have interfaced our Bonds with Soft Path LIS system. My justification for this started with using it for LEAN processes in that the orders for IHC went directly from the Pathologist to the Bond and eliminated the need for my techs to have to input individual orders for IHC by hand. Since we have set up our slide labelers to be recognized as just another printer as far as the LIS is concerned, we do not use paper labels at all but have 2d barcodes printed directly on our slides. When an order is put in by the pathologist for IHC, my techs can see the order, cut the section and print the slide with the correct bar code. Bond recognizes the barcode and initializes the tests that have been ordered and transferred from the pathologist. This has accomplished the following: No tech time lost in printing labels for slides to go on the bond. No ambiguity or lost IHC orders due to hand writing orders by the pathologist. No chance of keystroke errors on the part of my IHC tech while putting manual orders into the Bond. In addition to eliminating hand writing and manual keystrokes, which are distinct patient safety issue, I have calculated that having the interface has saved me approximately 0.7 FTE. Instead of having to hire extra staff to cover increased workloads or wasting existing staff on extraneous tasks (hand labeling, manually entering orders, etc), I can utilize them in other areas. The patient safety aspect of eliminating extra tasks involving manual data entry is huge. A majority of the lawsuits against pathology labs involve some aspect of human error resulting from manual tasks in labeling or data entry. In addition to being able to market my lab as patient safety focused, we have eliminated a major source of potential lawsuits. It's hard to put a price tag on what that saves other than to say that the costs are sometimes much more than the dollar figures paid out. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Thursday, October 21, 2010 4:01 PM To: Walter Benton Cc: Histonet Subject: Re: [Histonet] Clarification-interfacing the IHC bond and Cassettelabelers to Co-Path Hi Walter and Histo-subscribers, Ist I want to thank Walter for his quick reply. I appreciate your answer! 2nd, I appreciate any and all replies, but does anyone have an "article" that addresses issues that can occur such as: Efficiency Omitting Duplication of Tests ordered: Additional Slides, Special Stains, IHC, FISH, CISH, Cost effectiveness due to omission of errors Patient Safety Thanks Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Oct 21, 2010, at 11:38 AM, Walter Benton wrote: > Efficiency > Patient Safety > Orders for the Bond come directly from the LIS and can not be > misunderstood due to poor handwriting, since they are interfaced with > the LIS. > > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 126 > (All Deliveries to Suite 127) > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > wbenton@cua.md > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison > [akemiat3377@yahoo.com] > Sent: Thursday, October 21, 2010 2:33 PM > To: Histonet > Subject: [Histonet] interfacing the IHC bond and Cassette labelers > to Co-Path > > Hi out there in Histo Land! > > I would like your assistance in answering a question that was > proposed by a friend who is not a histonet member. I don't have the > answer, but know that one of you would. Below is the question: > > Could you help me justify the importance of interfacing our IHC bond > and Cassette labelers to Co-Path? A simple paragraph, or if you > have, a white paper, that would be great. I am attempting to get the > interfaces approved through our IT Department and running up against > some roadblocks. > > Thank you in advance for your assistance, > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From jshea121 <@t> roadrunner.com Fri Oct 22 13:06:31 2010 From: jshea121 <@t> roadrunner.com (Shea's) Date: Fri Oct 22 13:06:33 2010 Subject: [Histonet] eosin in processor Message-ID: <505DF12A430A4FD9AE81005A5BBD65D6@JoannePC> This is the best kept secret....For those who have never tried this, you don't know what you are missing in the ease of embedding and cutting. We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100% alcohols, leaving the last 100% pure (of course there is carry over during the week), then we dump the first and rotate the other 2 each week. The one that we now moved into position #2 will get 10cc eosin). We send our IHC & FISH to a HUGE reference lab. I specifically asked about this interfering and the technical rep said that it is not a problem esp they see a lot of it and they know to avoid non specific perimeter staining (the Pathologists know how to read around this because many labs are using Eosin in the processors). J From liz <@t> premierlab.com Fri Oct 22 13:11:03 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 22 13:08:34 2010 Subject: [Histonet] Cheap slide scanner for rat brains? In-Reply-To: Message-ID: Caroline The older models of the Nikon scanner for kodachromes had an attachment that would scan glass slides, I'm not sure about the new ones but we had one back in the late 90's early 2000's that would hold a slide and we could scan that. You could also take some low mag images on a microscope with a camera and them merge the images in adobe elements. It will work but the method can be a bit crude and you have to watch your illumination through the entire process. I have even used a regular digital camera and taken images of the glass slide, that worked too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: Friday, October 22, 2010 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cheap slide scanner for rat brains? Hello Everyone, Does anyone have a good way to get low magnification images from slides? I have some DAB stained rat coronal sections that I would like to digitize. Basically, I want to have a picture of the entire section. It doesn?t have to be high resolution, just enough to make out the basic structures. Can someone recommend a way to do this? I?ve used a flat bed scanner in the past, and I know some folks use an actual film slide scanner. What I?m interested in are tips for finding a solution, particularly what to avoid, and model numbers that people have used. I don?t want to keep buying scanners until I found one that works. And obviously I?d like to keep the expenses to a minimum. Finally, has anyone tried this scanner? It?s a little pricey for me, but I do like the idea behind it, although I wonder if it?s just one of their stock scanners that they have repainted, added an adapter and jacked up the price for. It looks identical to other scanners that they sell for $300. http://www.meyerinst.com/html/oem/pse4/ Thanks, Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Oct 22 13:09:36 2010 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Oct 22 13:10:11 2010 Subject: [Histonet] eosin in processor In-Reply-To: <505DF12A430A4FD9AE81005A5BBD65D6@JoannePC> Message-ID: Does anyone use eosin in their Peloris? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shea's Sent: Friday, October 22, 2010 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin in processor This is the best kept secret....For those who have never tried this, you don't know what you are missing in the ease of embedding and cutting. We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100% alcohols, leaving the last 100% pure (of course there is carry over during the week), then we dump the first and rotate the other 2 each week. The one that we now moved into position #2 will get 10cc eosin). We send our IHC & FISH to a HUGE reference lab. I specifically asked about this interfering and the technical rep said that it is not a problem esp they see a lot of it and they know to avoid non specific perimeter staining (the Pathologists know how to read around this because many labs are using Eosin in the processors). J _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From victor <@t> pathology.washington.edu Fri Oct 22 13:15:12 2010 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Oct 22 13:15:22 2010 Subject: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path In-Reply-To: References: Message-ID: <4CC1D4B0.1020308@pathology.washington.edu> Toni, We will be going live with the Bond shortly and will have the same workflow with a different LIS. Our pathologists have been ordering their own tests for years so there is no impact, except to save time in the Immuno Lab. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/22/2010 10:56 AM, Rathborne, Toni wrote: > I'd be interested in knowing how this has impacted your pathologists. Were they resistant at first? The extra time inputting requests would now fall on them. And there is still a chance of keystroke errors with pathologist entry. > I'm not disagreeing with the comments you made, only wondering since you were able to save .7 FTE, where the .7 pathologist came from. Or is it the interface itself that saves the time, and not who enters it? > Also, did you have the slide printers before the LEAN process was implemented? or was it done because of LEAN and is part of the FTE savings? > I'm very interested in this process, but know the types of questions I'll need to have answers for. We're a Cerner facility, so some things will be different but the principal will be the same. > > Toni > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Feher, > Stephen > Sent: Friday, October 22, 2010 1:32 PM > To: Akemi Allison; Walter Benton > Cc: Histonet > Subject: RE: [Histonet] Clarification-interfacing the IHC bond > andCassettelabelers to Co-Path > > > We have interfaced our Bonds with Soft Path LIS system. My > justification for this started with using it for LEAN processes in that > the orders for IHC went directly from the Pathologist to the Bond and > eliminated the need for my techs to have to input individual orders for > IHC by hand. Since we have set up our slide labelers to be recognized > as just another printer as far as the LIS is concerned, we do not use > paper labels at all but have 2d barcodes printed directly on our slides. > When an order is put in by the pathologist for IHC, my techs can see the > order, cut the section and print the slide with the correct bar code. > Bond recognizes the barcode and initializes the tests that have been > ordered and transferred from the pathologist. > > This has accomplished the following: > > No tech time lost in printing labels for slides to go on the bond. No > ambiguity or lost IHC orders due to hand writing orders by the > pathologist. No chance of keystroke errors on the part of my IHC tech > while putting manual orders into the Bond. In addition to eliminating > hand writing and manual keystrokes, which are distinct patient safety > issue, I have calculated that having the interface has saved me > approximately 0.7 FTE. Instead of having to hire extra staff to cover > increased workloads or wasting existing staff on extraneous tasks (hand > labeling, manually entering orders, etc), I can utilize them in other > areas. > > The patient safety aspect of eliminating extra tasks involving manual > data entry is huge. A majority of the lawsuits against pathology labs > involve some aspect of human error resulting from manual tasks in > labeling or data entry. In addition to being able to market my lab as > patient safety focused, we have eliminated a major source of potential > lawsuits. It's hard to put a price tag on what that saves other than to > say that the costs are sometimes much more than the dollar figures paid > out. > > > Steve > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi > Allison > Sent: Thursday, October 21, 2010 4:01 PM > To: Walter Benton > Cc: Histonet > Subject: Re: [Histonet] Clarification-interfacing the IHC bond and > Cassettelabelers to Co-Path > > Hi Walter and Histo-subscribers, > > Ist I want to thank Walter for his quick reply. I appreciate your > answer! 2nd, I appreciate any and all replies, but does anyone have an > "article" that addresses issues that can occur such as: > > Efficiency > Omitting Duplication of Tests ordered: Additional Slides, Special > Stains, IHC, FISH, CISH, Cost effectiveness due to omission of errors > Patient Safety > > Thanks > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Oct 21, 2010, at 11:38 AM, Walter Benton wrote: > >> Efficiency >> Patient Safety >> Orders for the Bond come directly from the LIS and can not be >> misunderstood due to poor handwriting, since they are interfaced with >> the LIS. >> >> >> Walter Benton HT(ASCP)QIHC >> Histology Supervisor >> Chesapeake Urology Associates >> 806 Landmark Drive, Suite 126 >> (All Deliveries to Suite 127) >> Glen Burnie, MD 21061 >> 443-471-5850 (Direct) >> 410-768-5961 (Lab) >> 410-768-5965 (Fax) >> wbenton@cua.md >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu [histonet- >> bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison >> [akemiat3377@yahoo.com] >> Sent: Thursday, October 21, 2010 2:33 PM >> To: Histonet >> Subject: [Histonet] interfacing the IHC bond and Cassette labelers >> to Co-Path >> >> Hi out there in Histo Land! >> >> I would like your assistance in answering a question that was >> proposed by a friend who is not a histonet member. I don't have the >> answer, but know that one of you would. Below is the question: >> >> Could you help me justify the importance of interfacing our IHC bond >> and Cassette labelers to Co-Path? A simple paragraph, or if you >> have, a white paper, that would be great. I am attempting to get the >> interfaces approved through our IT Department and running up against >> some roadblocks. >> >> Thank you in advance for your assistance, >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Fri Oct 22 13:42:05 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 22 13:42:16 2010 Subject: [Histonet] Cheap slide scanner for rat =?UTF-8?Q?brains=3F?= Message-ID: <20101022114205.9e2d9aa830e8449a2412eb1e4f2f067e.3010ec4cab.wbe@email04.secureserver.net> Do you have a dissecting scope you could use? You can grab one with a pretty low resolution camera for about $800. If you need more details let me know. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] Cheap slide scanner for rat brains? From: Caroline Bass Date: Fri, October 22, 2010 10:50 am To: "histonet@lists.utsouthwestern.edu" Hello Everyone, Does anyone have a good way to get low magnification images from slides? I have some DAB stained rat coronal sections that I would like to digitize. Basically, I want to have a picture of the entire section. It doesn?t have to be high resolution, just enough to make out the basic structures. Can someone recommend a way to do this? I?ve used a flat bed scanner in the past, and I know some folks use an actual film slide scanner. What I?m interested in are tips for finding a solution, particularly what to avoid, and model numbers that people have used. I don?t want to keep buying scanners until I found one that works. And obviously I?d like to keep the expenses to a minimum. Finally, has anyone tried this scanner? It?s a little pricey for me, but I do like the idea behind it, although I wonder if it?s just one of their stock scanners that they have repainted, added an adapter and jacked up the price for. It looks identical to other scanners that they sell for $300. http://www.meyerinst.com/html/oem/pse4/ Thanks, Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Fri Oct 22 14:44:34 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Fri Oct 22 14:44:46 2010 Subject: [Histonet] formalin containers Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5758@MAIL1.pph.local> We haven't heard anything about this (and we were inspected at the end of July). If you have any additional information, please post it. It is a little hard to image a "locking Lid" on a disposable plastic container of reasonable cost that is large enough to hold a a total colon resection or large mastectomy. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From Sandra.Harrison3 <@t> va.gov Fri Oct 22 14:54:53 2010 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Oct 22 14:54:59 2010 Subject: [Histonet] Block release In-Reply-To: <0AD641BB647144BCBC1AE4CD68C18B22@lurie.northwestern.edu> References: <0AD641BB647144BCBC1AE4CD68C18B22@lurie.northwestern.edu> Message-ID: Here's what the recently revised CAP checklist says about releasing blocks; scroll down to Note 2: **REVISED** 06/17/2010 ANP.12500 Record Retention Phase II Surgical pathology records and materials are retained for an appropriate period. NOTE 1: Minimum requirements for surgical pathology, providing these are not less stringent than state and federal regulations, are: 1. Accession log records - 2 years 2. Wet tissue (stock bottle) - 2 weeks after final report 3. Paraffin blocks - 10 years (subject to Note 2, below) 4. Glass slides (including control slides) and reports - 10 years (slides must remain readable for this period) 5. Surgical pathology reports - 10 years (see Notes 3 and 4, below) 6. Fluorochrome-stained slides - at the discretion of the laboratory director 7. Fine needle aspiration slides - 10 years 8. Images of FISH studies - 10 years (see Note 5, below) There must be a documented policy for protecting and preserving the integrity and retrieval of surgical pathology materials and records.The retention period should be extended, when appropriate, to provide documentation for adequate quality control and medical care. NOTE 2: Regarding release of blocks for research purposes: Federal regulations require that a laboratory retain paraffin blocks for two years. The CLA requires, however, that they must be kept for at least 10 years. Nevertheless, blocks may be released for research purposes after the two-year regulatory requirement if all of the following criteria are met: 1. The written consent of the patient is obtained. For laboratories subject to U.S. regulations, the consent must include formal authorization in accordance with the requirements of HIPAA, if identifiable patient information is released. 2. The laboratory retains sufficient blocks to support the diagnosis for the full 10-year period. 3. Provision is made for retrieval by the laboratory of any blocks or material that remain after use in research, if the blocks or material are needed for diagnostic, legal, or other legitimate purposes. 4. The laboratory meets other relevant requirements including but not limited to the requirements of the institution, the directives of any applicable institutional review board (IRB) or similar entity; and state and local laws and regulations. NOTE 3: Pathology reports may be retained in either paper or electronic format. If retained in electronic format alone, however, the electronic reports must include a secure pathologist electronic signature. Images of paper reports--such as microfiche or PDF files--are acceptable. NOTE 4: Reports of outside consultations performed on cases from the laboratory (whether or not such consultation was requested by the laboratory) must be retained for 10 years after the date on which the original report was issued. NOTE 5: There is no retention requirement for images when the source slides remain readable for the required 10-year retention period. The 10-year retention requirement applies to images of slide preparations that are not readable for the 10-year period (e.g. FISH studies). 22 of 44 VA Med Ctr - Minneapolis Path & Lab Med Service 113 Anatomic Pathology Checklist 06.17.2010 Evidence of Compliance: ? Written record and specimen retention policy(ies) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis <@t> seattlechildrens.org Fri Oct 22 14:55:20 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri Oct 22 14:55:28 2010 Subject: [Histonet] Coverslip removal question Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C06055C8B@s107.childrens.sea.kids> Hi Histonetters When I soak my slides in xylene to remove the coverslip, do I then need to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and then H20. And then drying and re applying permount? Or can I go straight from xylene to drying to re-coversliping with permount? Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Fri Oct 22 14:59:38 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 22 14:59:43 2010 Subject: [Histonet] Coverslip removal question In-Reply-To: <7EA5752B2903B143A5B845DEA87D5D1C06055C8B@s107.childrens.sea.kids> Message-ID: <968826.46233.qm@web65712.mail.ac4.yahoo.com> No, once you have removed the coverslip, just wash?the section?thoroughly in xylene to remove any thick remnant of the old permount,?and reapply permount and cover. Ren? J. --- On Fri, 10/22/10, Lewis, Patrick wrote: From: Lewis, Patrick Subject: [Histonet] Coverslip removal question To: Histonet@lists.utsouthwestern.edu Date: Friday, October 22, 2010, 3:55 PM Hi Histonetters When I soak my slides in xylene to remove the coverslip, do I then need to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and then H20. And then drying and re applying permount? Or can I go straight from xylene to drying to re-coversliping with permount? Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115? OFFICE 000-000-0000? PAGER 000-000-0000? CELL 206-884-7311? FAX patrick.lewis@seattlechildrens.org OFFICE? 1900 9th Avenue Seattle, WA 98101 MAIL? ? ? M/S C9S-8, Seattle, WA 98101 WWW? ???seattlechildrens.org CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.? Any unauthorized review, use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Fri Oct 22 15:00:53 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Oct 22 15:01:00 2010 Subject: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path In-Reply-To: References: <0908FC0A43B87A4FB60EDCCA06AABC2426C73E@exchange.cmc-nh.org> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2426C744@exchange.cmc-nh.org> Our LIS specialists built a template into the pathologist screen for each case where they simply check off the boxes of the IHC they want on a case. It took them a little while to get used to not using post it notes for their orders but once they got used to it, they like it. It only takes them a second or two to check what they want. Once they click the "send order" box, the order goes directly to the IHC worksheet for that day. When the stains are done and the qc tech scans their bar codes, the "finished cases" icon will appear in the pathologists queue. The interface itself saves us tech time since most pathologist are going to simply fill in a form or use a post it to convey their orders. A tech then has to enter this info into a worksheet and into the Bond prior to running the IHC. We built our lab (only opened since Jan 19, 2010) and equipped it based on LEAN processes. In some areas the LIS supported LEAN, in other areas we needed to use work arounds (like connecting each slide and cassette labeler to it's own CPU so that to the system, they appear as another printer with an IP address). I am a firm believer that any lab (brand new or a retrofit) can be adapted to LEAN through process changes, equipment adaptations, and/or using existing software more fully. Steve -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Friday, October 22, 2010 1:57 PM To: Feher, Stephen; Akemi Allison; Walter Benton Cc: Histonet Subject: RE: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path I'd be interested in knowing how this has impacted your pathologists. Were they resistant at first? The extra time inputting requests would now fall on them. And there is still a chance of keystroke errors with pathologist entry. I'm not disagreeing with the comments you made, only wondering since you were able to save .7 FTE, where the .7 pathologist came from. Or is it the interface itself that saves the time, and not who enters it? Also, did you have the slide printers before the LEAN process was implemented? or was it done because of LEAN and is part of the FTE savings? I'm very interested in this process, but know the types of questions I'll need to have answers for. We're a Cerner facility, so some things will be different but the principal will be the same. Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Feher, Stephen Sent: Friday, October 22, 2010 1:32 PM To: Akemi Allison; Walter Benton Cc: Histonet Subject: RE: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path We have interfaced our Bonds with Soft Path LIS system. My justification for this started with using it for LEAN processes in that the orders for IHC went directly from the Pathologist to the Bond and eliminated the need for my techs to have to input individual orders for IHC by hand. Since we have set up our slide labelers to be recognized as just another printer as far as the LIS is concerned, we do not use paper labels at all but have 2d barcodes printed directly on our slides. When an order is put in by the pathologist for IHC, my techs can see the order, cut the section and print the slide with the correct bar code. Bond recognizes the barcode and initializes the tests that have been ordered and transferred from the pathologist. This has accomplished the following: No tech time lost in printing labels for slides to go on the bond. No ambiguity or lost IHC orders due to hand writing orders by the pathologist. No chance of keystroke errors on the part of my IHC tech while putting manual orders into the Bond. In addition to eliminating hand writing and manual keystrokes, which are distinct patient safety issue, I have calculated that having the interface has saved me approximately 0.7 FTE. Instead of having to hire extra staff to cover increased workloads or wasting existing staff on extraneous tasks (hand labeling, manually entering orders, etc), I can utilize them in other areas. The patient safety aspect of eliminating extra tasks involving manual data entry is huge. A majority of the lawsuits against pathology labs involve some aspect of human error resulting from manual tasks in labeling or data entry. In addition to being able to market my lab as patient safety focused, we have eliminated a major source of potential lawsuits. It's hard to put a price tag on what that saves other than to say that the costs are sometimes much more than the dollar figures paid out. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Thursday, October 21, 2010 4:01 PM To: Walter Benton Cc: Histonet Subject: Re: [Histonet] Clarification-interfacing the IHC bond and Cassettelabelers to Co-Path Hi Walter and Histo-subscribers, Ist I want to thank Walter for his quick reply. I appreciate your answer! 2nd, I appreciate any and all replies, but does anyone have an "article" that addresses issues that can occur such as: Efficiency Omitting Duplication of Tests ordered: Additional Slides, Special Stains, IHC, FISH, CISH, Cost effectiveness due to omission of errors Patient Safety Thanks Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Oct 21, 2010, at 11:38 AM, Walter Benton wrote: > Efficiency > Patient Safety > Orders for the Bond come directly from the LIS and can not be > misunderstood due to poor handwriting, since they are interfaced with > the LIS. > > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 126 > (All Deliveries to Suite 127) > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > wbenton@cua.md > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison > [akemiat3377@yahoo.com] > Sent: Thursday, October 21, 2010 2:33 PM > To: Histonet > Subject: [Histonet] interfacing the IHC bond and Cassette labelers to > Co-Path > > Hi out there in Histo Land! > > I would like your assistance in answering a question that was proposed > by a friend who is not a histonet member. I don't have the answer, > but know that one of you would. Below is the question: > > Could you help me justify the importance of interfacing our IHC bond > and Cassette labelers to Co-Path? A simple paragraph, or if you have, > a white paper, that would be great. I am attempting to get the > interfaces approved through our IT Department and running up against > some roadblocks. > > Thank you in advance for your assistance, > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From patrick.lewis <@t> seattlechildrens.org Fri Oct 22 15:06:10 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri Oct 22 15:06:18 2010 Subject: [Histonet] Recommendations for decal solution Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C06055C9F@s107.childrens.sea.kids> Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From liz <@t> premierlab.com Fri Oct 22 15:13:57 2010 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 22 15:11:44 2010 Subject: [Histonet] Recommendations for decal solution In-Reply-To: <7EA5752B2903B143A5B845DEA87D5D1C06055C9F@s107.childrens.sea.kids> Message-ID: I like 5 to 10% formic acid Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Friday, October 22, 2010 2:06 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Recommendations for decal solution Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kc <@t> ka-recruiting.com Fri Oct 22 15:15:14 2010 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Fri Oct 22 15:15:20 2010 Subject: [Histonet] Lab Manager and other Histology Jobs Message-ID: <228115031.1287778514061.JavaMail.cfservice@SL4APP4> I am one of the founders of a healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you or anyone you know is interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Laboratory Manager opening at a Dermpath Lab. My client is a Pre-IPO Pathology company who is quickly establishing itself as an industry leader. They are looking for someone to step into a Lab Manager position that they have open in their Dermpath Lab in Long Island, NY. This state of the art lab s looking for someone with prior management experience to run and manage a lab consisting of 10 FTE's. This Manager will be responsible for overseeing the day to day operations of the lab. My clients offers one of the best compensation & benefits package around including relocation assistance when necessary. For consideration you must have a Bachelor's Degree, be HT or HTL (ASCP) certified and licensed in the State of NY. A minimum of 2 years of prior supervisory or management experience is preferred. If you are interested in learning more about this position, please call or email me at kc@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Histology Openings: NY - Upstate - Histotechnologist MI - Histology Manager NY - New York City - Histotechnologist - 3rd shift OK - Histology Supervisor GA - Histology Supervisor NV - Las Vegas - Histotechnologist - 3rd shift TN - Histotechnologist TX - Histology Supervisor If you're interested in any of these opportunities or are currently looking for employment then please send me an updated resume and let me know when you're available to talk. If this is not the right time for you to make a career change then please let me know if you can refer someone who is. We offer a generous referral bonus for anyone you refer to us that we place into any position across the country. To learn more about job openings in other parts of the country please contact me or visit our website at www.ka-recruiting.com. Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From caymanfleck <@t> gmail.com Fri Oct 22 15:16:50 2010 From: caymanfleck <@t> gmail.com (caymanfleck@gmail.com) Date: Fri Oct 22 15:16:53 2010 Subject: [Histonet] Tissue Processor Advice Message-ID: We are in need of some advice regarding rapid tissue processors. Models we are considering: Sakura Xpress Leica Peloris Thermo STP 420 It seems none of these models are perfect in every respect. I'm interested in anyone's opinions of these processors and your experience with them. All input is appreciated! From foreightl <@t> gmail.com Fri Oct 22 15:28:32 2010 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Oct 22 15:28:37 2010 Subject: [Histonet] eosin in processor In-Reply-To: References: <505DF12A430A4FD9AE81005A5BBD65D6@JoannePC> Message-ID: We don't, Leica recommends that you don't. We have our grossing group squirt a 0.1% Basic Fuchsin on the small translucent or white specimens. It leaves it light pink like the eosin. On Fri, Oct 22, 2010 at 11:09 AM, Rathborne, Toni < trathborne@somerset-healthcare.com> wrote: > Does anyone use eosin in their Peloris? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shea's > Sent: Friday, October 22, 2010 2:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] eosin in processor > > > This is the best kept secret....For those who have never tried this, you > don't know what you are missing in the ease of embedding and cutting. > > We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100% > alcohols, leaving the last 100% pure (of course there is carry over during > the week), then we dump the first and rotate the other 2 each week. The one > that we now moved into position #2 will get 10cc eosin). > > We send our IHC & FISH to a HUGE reference lab. I specifically asked about > this interfering and the technical rep said that it is not a problem esp > they see a lot of it and they know to avoid non specific perimeter staining > (the Pathologists know how to read around this because many labs are using > Eosin in the processors). > > J > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From patrick.lewis <@t> seattlechildrens.org Fri Oct 22 15:28:34 2010 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri Oct 22 15:28:44 2010 Subject: [Histonet] decal solution help Message-ID: <7EA5752B2903B143A5B845DEA87D5D1C06055CBF@s107.childrens.sea.kids> Dear Histonetters Yes I should have said, I'll be doing IHC for viral proteins with both polyclonal (rabbit) and monoclonal (Rat/Mouse) primary antibodies and HRP-labeled secondary antibodies with DAKO AEC Substrate. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sgoebel <@t> xbiotech.com Fri Oct 22 15:54:16 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Fri Oct 22 15:54:22 2010 Subject: [Histonet] Recommendations for decal solution Message-ID: <20101022135416.9e2d9aa830e8449a2412eb1e4f2f067e.77a189c146.wbe@email04.secureserver.net> Best decal in the world...Formical-4. It has formalin in it so it fixes and decals at the same time. You can get plain Jane decal too. The company is called decal. Here is the website. I think you can also get it from Fisher? http://www.decal-bone.com/ Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] Recommendations for decal solution From: "Lewis, Patrick" Date: Fri, October 22, 2010 1:06 pm To: Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 22 15:53:37 2010 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 22 16:00:24 2010 Subject: [Histonet] Recommendations for decal solution In-Reply-To: <7EA5752B2903B143A5B845DEA87D5D1C06055C9F@s107.childrens.sea.kids> Message-ID: <75443.40364.qm@web65713.mail.ac4.yahoo.com> For the specimens you mention use EDTA pH7 Ren? J. --- On Fri, 10/22/10, Lewis, Patrick wrote: From: Lewis, Patrick Subject: [Histonet] Recommendations for decal solution To: Histonet@lists.utsouthwestern.edu Date: Friday, October 22, 2010, 4:06 PM Hi guys, Can anyone recommend a good decal solution.? I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115? OFFICE 000-000-0000? PAGER 000-000-0000? CELL 206-884-7311? FAX patrick.lewis@seattlechildrens.org OFFICE? 1900 9th Avenue Seattle, WA 98101 MAIL? ? ? M/S C9S-8, Seattle, WA 98101 WWW? ???seattlechildrens.org CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.? Any unauthorized review, use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfeher <@t> CMC-NH.ORG Fri Oct 22 16:11:49 2010 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Oct 22 16:11:53 2010 Subject: [Histonet] Tissue Processor Advice In-Reply-To: References: Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2426C749@exchange.cmc-nh.org> We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol. We run 2 hour protocols throughout the day with an average of 4-5 runs per day depending on specimen volume. We really like this processor. We have had them for 10 months now, are using factory protocols and have not had any specimens that have been either under or over processed. The techs and the pathologists are very pleased with it. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caymanfleck@gmail.com Sent: Friday, October 22, 2010 4:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Advice We are in need of some advice regarding rapid tissue processors. Models we are considering: Sakura Xpress Leica Peloris Thermo STP 420 It seems none of these models are perfect in every respect. I'm interested in anyone's opinions of these processors and your experience with them. All input is appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Fri Oct 22 16:15:37 2010 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Oct 22 16:14:35 2010 Subject: [Histonet] Tissue Processor Advice In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC2426C749@exchange.cmc-nh.org> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C6923@EXCHANGECLUSTER.yumaregional.local> How are you meeting the hours of fixation requirement for Breast? With 2 and 4 and 8 hours,, But recently there are articles calling for Her 2 to be done on GI cases. Just want to know you insight on this? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, October 22, 2010 2:12 PM To: caymanfleck@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processor Advice We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol. We run 2 hour protocols throughout the day with an average of 4-5 runs per day depending on specimen volume. We really like this processor. We have had them for 10 months now, are using factory protocols and have not had any specimens that have been either under or over processed. The techs and the pathologists are very pleased with it. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caymanfleck@gmail.com Sent: Friday, October 22, 2010 4:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Advice We are in need of some advice regarding rapid tissue processors. Models we are considering: Sakura Xpress Leica Peloris Thermo STP 420 It seems none of these models are perfect in every respect. I'm interested in anyone's opinions of these processors and your experience with them. All input is appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From PMonfils <@t> Lifespan.org Fri Oct 22 17:27:16 2010 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Oct 22 17:27:24 2010 Subject: [Histonet] Aqua-Mount? Message-ID: <4EBFF65383B74D49995298C4976D1D5E074E9405@LSRIEXCH1.lsmaster.lifespan.org> Is Aqua-Mount aqueous mounting medium still available? If so, who sells it? If not, recommendations for a similar product? Thanks Paul From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Oct 22 19:08:03 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Oct 22 19:08:53 2010 Subject: [Histonet] RE: Aqua-Mount? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E074E9405@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E074E9405@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: We use an aqueous mounting media from Diagnostic Biosystems. Available in two sizes. Same as the aqua mount Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul [PMonfils@Lifespan.org] Sent: Friday, October 22, 2010 6:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Aqua-Mount? Is Aqua-Mount aqueous mounting medium still available? If so, who sells it? If not, recommendations for a similar product? Thanks Paul _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Fri Oct 22 23:40:53 2010 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Oct 22 23:41:00 2010 Subject: [Histonet] Tissue Processor Advice In-Reply-To: References: Message-ID: There are so many good to great processors on the market, but all have their plus and minus issues. You really have to decide what your two or three most important issues will be and then rank them. With the trend in becoming more efficient/cost effective, reducing TAT and LEAN process improvement, I suggest you look to improve your process to match these trends and you will be lead to rapid tissue processing in a LEAN way. Couple the previously mentioned trends/issues with versatility of processing with your routine formalin fixed samples with molecular fixed samples on the same instrument, I suggest the Sakura Xpress (X50 or X120) rapid processors. These instruments provide continuous loading, small batch and require a small volume of reagent for processing and then discard. The instruments do have a required reagent kit and there is a variable pre-processing protocol, depending on the tissue type and fat content. Using the reagent kit does allow for cost savings over conventional processing and I find the pre-processing allows for better precision processing techniques and protocols, we have never over processed tissues. Another great advantage is the increased velocity of the workflow as the instruments are continuous load (no cleaning cycle between batches) and small batch (1 to 40 cassettes). Loading 1 or 2 cassettes when a STAT or RUSH cases arrives and completes fixation does not interrupt the process or require special handling. An important factor to consider is that continuous load processing does assist in workload leveling, which can assist in reducing employee stress, increase productivity and error reduction. All these things lead directly to reduced TAT. Add the often overlooked advantage of removing Xylene from your tissue processing, and again, I suggest you consider the Xpress. I was an early adopter (5+ yrs use) and continue to use the X120 (2 units). I have not experienced any instrument performance or maintenance issues. I have had three software upgrades and the instruments had to go down for several hours to install the upgrade. The X120 and now the X50 have two programming options 1+ hour or 2+ hour processing. The most LEAN factor is that after the first basket of up to 40 cassettes, the next one comes off 20 or 40 minutes later and you can continuously load. There is no other instrument that can allow you to process in as small as batch or provide the continuous delivery of cassettes. You can do rapid processing with all of the instruments you are considering, but conventional, one reaction chamber instruments will limit the number of times the instrument can be run each day and that increases the batch size. Rapid processing does demand change in the way your lab does it's work. The first is standardization of gross dissection to <3 mm thickness. It does not matter the instrument, if you want to truly move into rapid processing, then you have to standardize your process in the gross room. The process of retraining and standardizing your gross room is well worth the effort. Another issue that you have to fully consider is how will specific fixation times affect your workflow. We now have specific guidelines for ER/PgR; Her2 and if you follow the NCI protocols for cancer tumors, you have to record your actual fixation times. Trying to manage all the different fixation times becomes difficult and will slow down your process. If you use your tissue processor to complete fixation, the processor will force you into larger batches. When you have to wait for tissues to complete fixation before starting your processing program, you also limit the number of times the processor can be run in a day. I find that separating fixation from processing is the best approach. You process specimens only when the optimal fixation time has been completed. Stop and consider all the different tissue types, size, fat content and required protocols, and you will see the value of a rapid processor that has small batch and continuous load capabilities. Meet all your fixation requirements and needs and only use the tissue processor for processing, not fixation. This is very LEAN concept and a concept that I believe you will need to embrace. Whenever you have the opportunity to change your process, I always suggest you look to improve the process, use the latest process improvement techniques and select an instrument that will assist in the change and prepare you for future change. My philosophy is we cannot continue to do tomorrow what we do today and expect a different outcome or result. Just my thoughts and experience, I hope this will help you. Do not hesitate to contact me if you have any questions. William DeSalvo, B.S., HTL(ASCP) > Date: Fri, 22 Oct 2010 16:16:50 -0400 > From: caymanfleck@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Tissue Processor Advice > > We are in need of some advice regarding rapid tissue processors. Models we > are considering: > > Sakura Xpress > Leica Peloris > Thermo STP 420 > > It seems none of these models are perfect in every respect. I'm interested > in anyone's opinions of these processors and your experience with them. > > All input is appreciated! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Sat Oct 23 08:26:15 2010 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Sat Oct 23 08:26:28 2010 Subject: [Histonet] Wear gloves when handling / cutting paraffin blocks. Message-ID: <22328.119.152.84.168.1287840375.squirrel@brain.net.pk> Dear All, I am looking some kind of references out there that addresses that it is not a requirement to wear gloves when handling / cutting paraffin blocks. Any help would be greatly appreciated Thank in advance, Muhammad Tahseen Senior Supervisor Histology SKMCH&RC Pakistan From asmith <@t> mail.barry.edu Sat Oct 23 13:33:09 2010 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Sat Oct 23 13:36:37 2010 Subject: [Histonet] Wear gloves when handling / cutting paraffin blocks. In-Reply-To: <22328.119.152.84.168.1287840375.squirrel@brain.net.pk> References: <22328.119.152.84.168.1287840375.squirrel@brain.net.pk> Message-ID: Because the number of things that are required is finite, requirements can be documented. Because the number of things that are not required is infinite, it is usually impossible to find a printed statement that something is unnecessary. (I cannot document that gloves are unnecessary for handling paraffin blocks. I cannot document that a gas mask is unnecessary for handling paraffin blocks. I cannot document that a crash helmet is unnecessary for handling paraffin blocks. I cannot document that is unnecessary for the hospital to fly a purple flag while paraffin blocks are being cut.) I have never worn gloves when handling paraffin blocks. I have never seen anyone wear gloves to handle paraffin blocks. I suppose it might be wise to wear gloves while working with a paraffin block of a suspected case of Creutzfeld-Jacob disease or MDX tuberculosis. In all other cases, all organisms in the block are dead. Why bother with gloves? Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk Sent: Saturday, October 23, 2010 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wear gloves when handling / cutting paraffin blocks. Dear All, I am looking some kind of references out there that addresses that it is not a requirement to wear gloves when handling / cutting paraffin blocks. Any help would be greatly appreciated Thank in advance, Muhammad Tahseen Senior Supervisor Histology SKMCH&RC Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Sat Oct 23 18:33:36 2010 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sat Oct 23 18:33:40 2010 Subject: [Histonet] handling blocks (Muhammad's inquiry) Message-ID: <487023.52959.qm@web50907.mail.re2.yahoo.com> in consideration of the status of paraffin blocks... ? Assuming you've done a good job in fixing prior to and during processing and you don't encounter prion diseases, processed/blocked tissues?are?inert.? Theoretically, you could eat them with no adverse issues other than paraffin sticking to your teeth.??We've all?heard the story of a safety officer trying to convince a histology manager that blocks were biohazardous.? The safety officer was?certain gloves were needed and wasn't allowing any discussion until the manager picked up a block,? looked the S.O. in the eye?and licked the cut surface of the block.? End of discussion.? ? Have you considered if you declare these blocks contaminated, the impact for discarding them over time?? Cutting would then be a situation creating?aerosols so you'd need masks and negative pressure on the cutting room.?If you call them biohazardous, then you also have to discard them as biohazard waste, as well as all the fluids and paraffins and extraneous?waste?involved in processing and cutting?them.? Storage and transport of blocks, such as?to a?reference lab, would require biohazard tracking....etc. ?Do you really want to impact your lab this significantly for something that?has no documented history of?biohazard? (Unless you work with prion disease!!) ? Any verified cases of infection or problems out there?? ? We do know many people who cut and embed?with gloves?by choice, not as a requirement.? Most of us have decades of experience cutting and handling blocks barehanded...why create more work & expense?for no documented, proven reason? ? Cheryl Kerry, HT(ASCP) Houston, TX From JWeems <@t> sjha.org Sat Oct 23 18:39:17 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sat Oct 23 18:39:23 2010 Subject: [Histonet] handling blocks (Muhammad's inquiry) In-Reply-To: <487023.52959.qm@web50907.mail.re2.yahoo.com> References: <487023.52959.qm@web50907.mail.re2.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640448AF245E@CHEXCMS10.one.ads.che.org> The one reason other that prion potential diseases, is to eliminate epithelial from dry skin, if necessary. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Saturday, October 23, 2010 19:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] handling blocks (Muhammad's inquiry) in consideration of the status of paraffin blocks... ? Assuming you've done a good job in fixing prior to and during processing and you don't encounter prion diseases, processed/blocked tissues?are?inert.? Theoretically, you could eat them with no adverse issues other than paraffin sticking to your teeth.??We've all?heard the story of a safety officer trying to convince a histology manager that blocks were biohazardous.? The safety officer was?certain gloves were needed and wasn't allowing any discussion until the manager picked up a block,? looked the S.O. in the eye?and licked the cut surface of the block.? End of discussion.? ? Have you considered if you declare these blocks contaminated, the impact for discarding them over time?? Cutting would then be a situation creating?aerosols so you'd need masks and negative pressure on the cutting room.?If you call them biohazardous, then you also have to discard them as biohazard waste, as well as all the fluids and paraffins and extraneous?waste?involved in processing and cutting?them.? Storage and transport of blocks, such as?to a?reference lab, would require biohazard tracking....etc. ?Do you really want to impact your lab this significantly for something that?has no documented history of?biohazard? (Unless you work with prion disease!!) ? Any verified cases of infection or problems out there?? ? We do know many people who cut and embed?with gloves?by choice, not as a requirement.? Most of us have decades of experience cutting and handling blocks barehanded...why create more work & expense?for no documented, proven reason? ? Cheryl Kerry, HT(ASCP) Houston, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ccrowder <@t> vetmed.lsu.edu Sat Oct 23 22:31:30 2010 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Sat Oct 23 22:32:57 2010 Subject: [Histonet] Decal solution Message-ID: Hi - There are a myriad of decal solutions. You could contact Gayle Callis - she is an expert and has given workshops on bone and decaling for some time. We have used her suggestions of 15-20% formic acid for years, with no detrimental outcomes even for IHC. However, if you don't want to make the solution yourself, call several manufacturers, get samples and compare the results. Cheryl From lhadley <@t> iupui.edu Mon Oct 25 08:02:30 2010 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Mon Oct 25 08:02:37 2010 Subject: [Histonet] RE: Recommendations for decal solution In-Reply-To: <7EA5752B2903B143A5B845DEA87D5D1C06055C9F@s107.childrens.sea.kids> References: <7EA5752B2903B143A5B845DEA87D5D1C06055C9F@s107.childrens.sea.kids> Message-ID: <8638FBDA16B0584D82AA21CD236FF97F105E0FF1@IU-MSSG-MBX110.ads.iu.edu> 5% Formic Acid Thanks Lee Ann -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Friday, October 22, 2010 4:06 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Recommendations for decal solution Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.lewis@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Mon Oct 25 08:19:11 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Oct 25 08:19:15 2010 Subject: [Histonet] Great position for an HT in Nashville, TN Message-ID: <6F33D8418806044682A391273399860F05AF11CA@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From b-frederick <@t> northwestern.edu Mon Oct 25 08:29:26 2010 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Oct 25 08:29:49 2010 Subject: [Histonet] Wear gloves when handling / cutting paraffin blocks. In-Reply-To: <22328.119.152.84.168.1287840375.squirrel@brain.net.pk> Message-ID: We wear gloves we have to sterile section blocks for DNA/RNA extraction. Keeps the slides free from contamination if we are placing sections on slides, otherwise we are sticking them in a vial. All equipment associated with the sterile sectioning is cleaned between each block (block holder, blade holder, forceps etc) and gloves are changed. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen@brain.net.pk Sent: Saturday, October 23, 2010 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wear gloves when handling / cutting paraffin blocks. Dear All, I am looking some kind of references out there that addresses that it is not a requirement to wear gloves when handling / cutting paraffin blocks. Any help would be greatly appreciated Thank in advance, Muhammad Tahseen Senior Supervisor Histology SKMCH&RC Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Oct 25 08:49:39 2010 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCZVED)) Date: Mon Oct 25 08:51:28 2010 Subject: [Histonet] paraffin block mailers Message-ID: Hi everyone, Does anyone know of a source for packaging exclusive to mailing paraffin blocks? Vendors please feel free to reply as well. Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From AGleiberman <@t> cbiolabs.com Mon Oct 25 09:11:11 2010 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Mon Oct 25 09:11:16 2010 Subject: [Histonet] Aqua-Mount? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E074E9405@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E074E9405@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: It is available from this site: http://www.egeneralmedical.com/vwr-41799-008.html Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, October 22, 2010 6:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Aqua-Mount? Is Aqua-Mount aqueous mounting medium still available? If so, who sells it? If not, recommendations for a similar product? Thanks Paul _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From POWELL_SA <@t> mercer.edu Mon Oct 25 09:42:51 2010 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Oct 25 09:42:52 2010 Subject: [Histonet] RE: paraffin block mailers In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE22691FC2572@MERCERMAIL.MercerU.local> Hi Jeanine, At the NSH vendors I got a sample of a neat clear plastic holder from Source Medical Products, www.sourcemp.com, 1866-735-9965, holds 4 maybe 8 blocks for mailing, depending upon the thickness. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCZVED) Sent: Monday, October 25, 2010 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin block mailers Hi everyone, Does anyone know of a source for packaging exclusive to mailing paraffin blocks? Vendors please feel free to reply as well. Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Mon Oct 25 09:54:51 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Oct 25 09:55:00 2010 Subject: [Histonet] Microwave protocols In-Reply-To: Message-ID: <10539A8B16F24A408092413A3431F8C5@hopperPC> Hi Histonetters! I am back with some questions on protocols for microwave processing. We have the Milestone processor. For any of you out there who use the Milestone, do you find the pre-programmed processing runs reasonable for your use or have you modified them? If modified, would you be willing to share your modifications? Next thought, do you process your breast needle core biopsies/lumpectomies/mastectomies etc in the microwave? I understand that they would have to properly fix in formalin for the minimum of 6 hours, but do you then process conventionally or with the MW? Does anyone have any thoughts as to whether or not the microwave would compromise the Her2/ER/PR results? Does anyone have any other pitfalls/thoughts/concerns that I need to keep in mind when using the microwave processors? THANKS for your help! Michelle From JWatson <@t> gnf.org Mon Oct 25 10:04:23 2010 From: JWatson <@t> gnf.org (James Watson) Date: Mon Oct 25 10:04:49 2010 Subject: [Histonet] Temporary Scientific Associate, Histology, spring 2011 Message-ID: GNF is currently seeking a temporary Scientific Associate to join the Histology group, for the beginning of March 2011 through the end of July 2011. Perform necropsies, tissue processing, slide sectioning, routine staining, special staining, immunohistochemical staining and complex procedures necessary in preparing specimens of animal tissue in a research environment. Qualifications: Associate's or B.S. degree in a biological science or completion of a NAACLS accredited School of Histotechnology is required. American Society of Clinical Pathologist (ASCP) certification as a HT or HTL is required. Applicants must demonstrate the potential ability to perform the essential functions of the job as outlined in the position description. The employee must have 2-5 years of experience in the Histology lab performing animal techniques, a wide variety of manual histochemical and enzymatic staining, automated and manual immunohistochemistry, and automated and manual in-situ hybridization. Essential Functions: 1. Identifies significant tissue elements microscopically to determine quality of staining. 2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. 3. Performs Microtomy on rotary microtome, cryostat, and be able to use a sliding microtome. 4. Prepares dyes and solutions in order to perform routine, special, and complex procedures. 5. Have experience in histochemical stains and enzymatic histochemical stains. Be able to trouble shoot and correct problems with histochemical stains. Have experience in Immunohistochemical staining and other advanced histological procedures. 6. Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. 7. Operate Slide scanning Instrumentation. Interested candidates please send a copy of your CV with a summary of research experience, and three references. Job Code: JW10-013 Contact:E-mail: jobs@gnf.org James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From Valerie.Hannen <@t> parrishmed.com Mon Oct 25 10:39:56 2010 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Oct 25 10:39:59 2010 Subject: [Histonet] FW: Saponin Technique Message-ID: <5680DA93771F0C48954CC8D38425E72401AB3516@ISMAIL.parrishmed.local> ________________________________ From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histonet@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From TMcNemar <@t> lmhealth.org Mon Oct 25 10:52:31 2010 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Oct 25 10:52:16 2010 Subject: [Histonet] RE: Saponin Technique In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB3516@ISMAIL.parrishmed.local> Message-ID: I don't know about the procedure you have but ours only uses a 1% solution of saponin, a 3% solution of calcium gluconate, and a balanced salt solution. We get the balanced salt solution from our pharmacy. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, October 25, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Saponin Technique ________________________________ From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histonet@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Oct 25 10:51:11 2010 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Oct 25 10:54:45 2010 Subject: [Histonet] RE: Saponin Technique In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB3516@ISMAIL.parrishmed.local> References: <5680DA93771F0C48954CC8D38425E72401AB3516@ISMAIL.parrishmed.local> Message-ID: It's been a while but I think that we used to add a little diluted glacial acetic acid to our cytology specimens to lyse the red cells. Not sure on the dilution, but maybe someone else knows. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [Valerie.Hannen@parrishmed.com] Sent: Monday, October 25, 2010 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Saponin Technique ________________________________ From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histonet@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bill.Tench <@t> pph.org Mon Oct 25 10:55:56 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Mon Oct 25 10:56:05 2010 Subject: [Histonet] Saponin Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5764@MAIL1.pph.local> Lots of blood can be a problem in cyto specimens, especially smears. If you are making smears from fresh specimens in particular you may elute the obscuring hemoglobin by dipping the smear directly in acid alcohol (i think 5% hcl-95% etoh). We do this frequently for CT guided FNA's (particularly of the liver and thyroid, which tend to be bloody). We also do this "post facto" on those smears even when they have already been stained with H and E. The red cell stroma disappears into the background). Just lift the coverslip and back up to 95%, use acid alcohol ( i think it is sold on the commercial market as "differentiating agent"), and then start staining process all over. With fresh specimens you can see the hemoglobin elute from the surface while you dip the slide. We typically go back into regular 95% Etoh just to get rid of the acid background (effects the blueing down the line). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From candice_camille <@t> yahoo.com Mon Oct 25 11:08:15 2010 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Mon Oct 25 11:08:19 2010 Subject: [Histonet] A Good Human Brain Control Message-ID: <465164.93851.qm@web62207.mail.re1.yahoo.com> Hi Histonetters, I was wondering if any one could tell me where can I find a human brain amyloid control. I just need some suggestions on where to order them ( control slides) from. Please feel free to list any suggestions. Thanks From brandihiggins <@t> gmail.com Mon Oct 25 11:08:58 2010 From: brandihiggins <@t> gmail.com (Brandi Higgins) Date: Mon Oct 25 11:09:05 2010 Subject: Fwd: [Histonet] FW: Saponin Technique In-Reply-To: References: <5680DA93771F0C48954CC8D38425E72401AB3516@ISMAIL.parrishmed.local> Message-ID: ---------- Forwarded message ---------- From: Brandi Higgins Date: Mon, Oct 25, 2010 at 12:08 PM Subject: Re: [Histonet] FW: Saponin Technique To: "Hannen, Valerie" We use Carnoy's to lyse the red blood cells. We get thyroid and lymph node FNA's regularly and we always process the slides through the Carnoy's. The solution we use is 120 ml 95% alcohol, 60 ml chloroform, and 20 ml glacial acetic acid. This 200 ml solution is good for the regular sized container for a full rack of slides, but if you only have a few slides and not a whole rack you can use 12 ml, 6 ml, 2ml (or any ratio you want obviously) so as not to waste solution. We place slides in Carnoy's for 5 minutes. The literature says 3-5 minutes, and not to exceed 15 minutes and you may require more time in the hematoxlin following this procedure, although we have not found that to be necessary. Also please note that the solution must be prepared fresh before use. We already had glacal acetic acid on hand when we started using Carnoy's so we just had to order chloroform. Brandi Higgins, BS, H(ASCP) On Mon, Oct 25, 2010 at 11:39 AM, Hannen, Valerie < Valerie.Hannen@parrishmed.com> wrote: > > > ________________________________ > > From: Hannen, Valerie > Sent: Monday, October 25, 2010 11:39 AM > To: 'histonet@list.utsouthwestern.edu' > Subject: Saponin Technique > > > Hi folks.. > I am hoping someone out there in Histo-land can help. One of our > Pathologists is asking us to check into doing the Saponin Technique to > lyse the red cells in some of our cytology specimens. She gave a copy of > one of the pages from one of her manuals, and it shows that in order for > us to do this we would need to buy alot of reagents that we currently do > not have. Being that we would probably not be doing this technique very > often, and not sure how cost effective it will be for us to buy the > reagents, we are wondering if anyone knows of a commercial product that > we can buy that would be a cheaper way out. > > Thanks in advance!! > Valerie Hannen, Histotechnologist > Parrish Medical Center > Titusville,Florida > > > ************************************************************** > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > ************************************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Sharon.Davis-Devine <@t> carle.com Mon Oct 25 11:11:43 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Mon Oct 25 11:11:48 2010 Subject: [Histonet] RE: Saponin In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5764@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A5764@MAIL1.pph.local> Message-ID: Why use Saponin when you can get a commercially prepared solution such as Lysing 1000, which was specifically designed to remove rbc's from Cytology specimens. Lysing 1000 not only lyses the rbc's but gives you exceptionally preserved cellular material which is amiable with IHC staining. Lysing 1000 is available from Obiter Research, LLC (www.obires.com) and only costs $25 per gallon. We have used it on all of our non-Gyn and FNA specimens for over 12 years now. We use it instead of 95% alcohol for our FNA smears. We prepare a smear, air dry one for a diff quik stain and immediately immerse the other on in a Coplin jar of Lysing 1000. It removes the rbc's while immediately fixing the cells present on the slide. I recommend using plus slides so the cells don't fall off. We then rinse the needle in a container of Lysing 1000 which lyses the rbc's and leaves you with a very cellular specimen for a cell block. We have valildated it's use with IHC staining using our Ventana Benchmarks and seem to get consistently excellent staining results on our cell blocks. I highly recommend it over Carnoys and Saponin. Let me know if you have any questions. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Monday, October 25, 2010 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Saponin Lots of blood can be a problem in cyto specimens, especially smears. If you are making smears from fresh specimens in particular you may elute the obscuring hemoglobin by dipping the smear directly in acid alcohol (i think 5% hcl-95% etoh). We do this frequently for CT guided FNA's (particularly of the liver and thyroid, which tend to be bloody). We also do this "post facto" on those smears even when they have already been stained with H and E. The red cell stroma disappears into the background). Just lift the coverslip and back up to 95%, use acid alcohol ( i think it is sold on the commercial market as "differentiating agent"), and then start staining process all over. With fresh specimens you can see the hemoglobin elute from the surface while you dip the slide. We typically go back into regular 95% Etoh just to get rid of the acid background (effects the blueing down the line). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> xbiotech.com Mon Oct 25 11:26:30 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Mon Oct 25 11:26:34 2010 Subject: [Histonet] A Good Human Brain Control Message-ID: <20101025092629.9e2d9aa830e8449a2412eb1e4f2f067e.15242e7617.wbe@email04.secureserver.net> American Matertech Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] A Good Human Brain Control From: Candice Smoots Date: Mon, October 25, 2010 9:08 am To: histonet@lists.utsouthwestern.edu Hi Histonetters, I was wondering if any one could tell me where can I find a human brain amyloid control. I just need some suggestions on where to order them ( control slides) from. Please feel free to list any suggestions. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From neelyk <@t> shands.ufl.edu Mon Oct 25 12:12:35 2010 From: neelyk <@t> shands.ufl.edu (Kendall Neely) Date: Mon Oct 25 12:12:41 2010 Subject: [Histonet] Thanks everyone! Message-ID: <4CC58243.3024.0059.1@shands.ufl.edu> Thank you to everyone who provided input on preferences for H&E stainers last week. You were all very helpful! Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 From relia1 <@t> earthlink.net Mon Oct 25 12:31:07 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Oct 25 12:31:49 2010 Subject: [Histonet] Director of Clinical Lab needed can you help? Message-ID: Hi Histonetters! I hope everyone is having a great day. I just got a new position that I want to tell you about . The position is Clinical Lab Director. The position is with a hospital system and you would have the lab managers from the 5 affiliated labs reporting to you. The position is in AR. Please let me know if you would be interested or know of someone who might be interested. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From vavalos <@t> allergydermatology.com Mon Oct 25 14:05:52 2010 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Mon Oct 25 14:06:01 2010 Subject: [Histonet] Pouring Chemicals in Stainer Message-ID: <000301cb7477$a8a61ce0$f9f256a0$@com> I was curious about any tricks out there for avoiding spills when setting up the stainer. We will be receiving a new stainer soon which will be taller and larger than our current one. I'm just worried I will spill chemical into the surrounding dishes. Would using wash bottles work or are there any other ideas out there? V.Avalos ADS, INC Fax:602-277-2134 From micropathlabs <@t> yahoo.com Mon Oct 25 14:07:10 2010 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Mon Oct 25 14:07:13 2010 Subject: [Histonet] Xylene Substitute Message-ID: <212063.2826.qm@web57805.mail.re3.yahoo.com> Hi all! I'm looking for recommendations for a xylene substitute for our processing and staining. We have two VIP's and a Leica stainer. We use Permount for coverslipping (manually with glass). Does anyone?use a?xylene substitute that?you would recommend for this combination? Thank?you! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From histotech <@t> imagesbyhopper.com Mon Oct 25 15:13:05 2010 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Oct 25 15:13:21 2010 Subject: [Histonet] Xylene Substitute In-Reply-To: <212063.2826.qm@web57805.mail.re3.yahoo.com> Message-ID: Sheila, You might want to talk to the Leica rep. My guy was here a couple of weeks ago and they have a xylene sub and a mouting media that plays nicely with it. I don't recall the name of the product, but it might be worth talking to him about it. Good Luck! Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, October 25, 2010 3:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute Hi all! I'm looking for recommendations for a xylene substitute for our processing and staining. We have two VIP's and a Leica stainer. We use Permount for coverslipping (manually with glass). Does anyone?use a?xylene substitute that?you would recommend for this combination? Thank?you! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3214 - Release Date: 10/24/10 18:34:00 From bbroders <@t> unlnotes.unl.edu Mon Oct 25 16:36:10 2010 From: bbroders <@t> unlnotes.unl.edu (Bruce W Brodersen) Date: Mon Oct 25 16:36:15 2010 Subject: [Histonet] Rubber cutting mats with adhesive backs Message-ID: Why did they quit making them and what are others using as a replacement? Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094 From TGoins <@t> mt.gov Mon Oct 25 17:06:43 2010 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Oct 25 17:06:49 2010 Subject: [Histonet] Xylene Substitute In-Reply-To: <212063.2826.qm@web57805.mail.re3.yahoo.com> References: <212063.2826.qm@web57805.mail.re3.yahoo.com> Message-ID: We use Clear-Rite 3 (Richard Allan Scientific 6901) from Fisher Scientific in our VIP processor and for staining. I am not familiar with Permount, but we use Richard-Allan Mounting Medium (4111; also available from Fisher Scientific) that is toluene based - works great in combination with Clear-Rite 3. Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, October 25, 2010 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute Hi all! I'm looking for recommendations for a xylene substitute for our processing and staining. We have two VIP's and a Leica stainer. We use Permount for coverslipping (manually with glass). Does anyone?use a?xylene substitute that?you would recommend for this combination? Thank?you! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Oct 25 17:42:36 2010 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Oct 25 17:42:50 2010 Subject: [Histonet] RE: Saponin Technique In-Reply-To: Message-ID: The following has been found useful in my hands: Removal of Excess Blood Haemolysing haemorrhagic specimens cause fewer problems in identifying individual cells. The removal of red blood cells can be achieved using Ficoll-Hypaque gradient separation or using a lysis solution such as isotonic ammonium chloride (Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479.): SOLUTIONS: 1. Lysis solution Ammonium Chloride 4.5g Potassium carbonate 0.5g EDTA 0.0186g Distilled water 500mls 2. Hanks medium METHOD: 1. Centrifuge bloody fluid. 2. Remove supernatant and add equal volume of lysis solution. 3. Resuspend and incubate for 5 minutes at 4oC. 4. Centrifuge, if blood still remains, then repeat from step 2. 5. Resuspend pellet in Hanks. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Tuesday, 26 October 2010 2:51 AM To: Hannen, Valerie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Saponin Technique It's been a while but I think that we used to add a little diluted glacial acetic acid to our cytology specimens to lyse the red cells. Not sure on the dilution, but maybe someone else knows. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [Valerie.Hannen@parrishmed.com] Sent: Monday, October 25, 2010 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Saponin Technique ________________________________ From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histonet@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From micropathlabs <@t> yahoo.com Tue Oct 26 06:38:49 2010 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Oct 26 06:38:52 2010 Subject: [Histonet] Thanks Message-ID: <526564.74797.qm@web57805.mail.re3.yahoo.com> Thanks to everyone for the information on xylene substitutes. This should help me get the ball rolling. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From kmerriam2003 <@t> yahoo.com Tue Oct 26 08:31:57 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Oct 26 08:32:01 2010 Subject: [Histonet] cryojane problems Message-ID: <983813.11449.qm@web50306.mail.re2.yahoo.com> Hi All, I have been using the cryojane to cut human articular cartilage, with excellent results.? Now I need to cut frozen sections of rat articular cartilage that?contain some underlying bone.??I am getting nice sections, but when I?peel the tape off the slide, a bunch of my tissue is staying on the tape.? I?have tried increasing the number of flashes (to a whopping 10!), hoping that this will help adhere the sections, but it hasn't. Any tips for me? Kim? ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From brett_connolly <@t> merck.com Tue Oct 26 08:41:18 2010 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Oct 26 08:41:23 2010 Subject: [Histonet] cryojane problems In-Reply-To: <983813.11449.qm@web50306.mail.re2.yahoo.com> References: <983813.11449.qm@web50306.mail.re2.yahoo.com> Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D73BD8A8@usctmx1176.merck.com> Kim, I would recommend using their '4x' slides - they have more adhesive on them. I don't think flashing more than once has any benefit. Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, October 26, 2010 9:32 AM To: Histonet Subject: [Histonet] cryojane problems Hi All, I have been using the cryojane to cut human articular cartilage, with excellent results.? Now I need to cut frozen sections of rat articular cartilage that?contain some underlying bone.??I am getting nice sections, but when I?peel the tape off the slide, a bunch of my tissue is staying on the tape.? I?have tried increasing the number of flashes (to a whopping 10!), hoping that this will help adhere the sections, but it hasn't. Any tips for me? Kim? ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From mhale <@t> carisls.com Tue Oct 26 10:23:09 2010 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Oct 26 10:23:17 2010 Subject: [Histonet] Nashville TN HT Position Message-ID: <6F33D8418806044682A391273399860F05B70DDF@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From relia1 <@t> earthlink.net Tue Oct 26 11:20:16 2010 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Oct 26 11:20:22 2010 Subject: [Histonet] RELIA Histology Careers Bulletin 10-26-10 Happy Halloween and Trick or Treat! Message-ID: Hi Histonetters! The leaves are changing; there is a crispness in the air. I hope you are enjoying a wonderful Autumn. Halloween is just around the corner TRICK or TREAT!!!!! The TRICK is finding the right job opportunity for you. The TREAT is with my help it can be relatively painless. * I will help you with your resume, * Coach you through the interview and offer process * And refer you to positions based on the criteria you give me. All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. Here is a list of my current openings: MANAGEMENT AR ? Clinical Lab Director CA ? Histology Lab Manager NY-Quality Assurance Manager HISTOTECHNICIANS/HISTOTECHNOLOGISTS MD- Near Hagerstown day shift state of the art lab TN ? Nashville Histotech day and night shifts avail. FL- Central Florida Histology Product Sales IN ? Indianapolis Dermpath exper preferred IN ? South of Chicago in Northern IN Histology Tech GA ? Southern Coastal area ? Histotech with IHC ASCP HTL required. NY- Suffern NYS license/Elig day and night shift MA ? Boston Area several shifts available dermpath and general FL ? Orlando State of FLTechnologist license and 2 yrs exper req. RESEARCH CA ? Redding Inside Technical Support with TEM WI ? Research Histology Tech If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open even if you are happy in your present job. Happy Halloween!!!!!!!!!!!! Pam ? 866-607-3542 (866-60RELIA) p.s. What are you going to be for Halloween? Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Mandy.Bell <@t> chomp.org Tue Oct 26 11:24:51 2010 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Tue Oct 26 11:25:21 2010 Subject: [Histonet] M. Tuberculosis antibody Message-ID: Hi Histonetters, I was wondering if anyone knew of a Mycobacterium Tuberculosis antibody that wasn't an RUO, and could be used on the Ventana Benchmark XT. Thanks for your help! Mandy M Bell , HTL (ASCP) P Please consider the environment before printing this e-mail From ahacking <@t> yahoo.com Tue Oct 26 14:21:06 2010 From: ahacking <@t> yahoo.com (Adam Hacking) Date: Tue Oct 26 14:21:10 2010 Subject: [Histonet] (no subject) Message-ID: <234859.63869.qm@web30803.mail.mud.yahoo.com> Hi, ? Does anyone have a blade holder, tungesten blade and clamp for holding specimens that would fit on a Leica 2265 ? I'm trying to upgrade?a?microtome set up for paraffin sectioning to one that can handle resin embedded sections. ? Many thanks ? Adam From njblademaster <@t> gmail.com Tue Oct 26 14:43:28 2010 From: njblademaster <@t> gmail.com (Nathan Jentsch) Date: Tue Oct 26 14:53:32 2010 Subject: [Histonet] RE: xylene substitute Message-ID: We use a product called pro-par, produced by Anatech. We don't use permount routinely, but a few of our specials require it. We dehydrate and then clear with the pro-par, and it works fine for those stains. It is much less harsh on tissue than xylene; we have three VIP's, and our specimens sit in it for several hours with no negative effects on the tissue or the processors. Nathan Jentsch BS HT(ASCP) From Jacqueline.Farnsworth <@t> cls.ab.ca Tue Oct 26 15:56:02 2010 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Tue Oct 26 15:56:17 2010 Subject: [Histonet] gram stain/label issues Message-ID: HI all, We are doing gram stains manually, and the acetone is removing some of the 'ink' from the printed labels making them difficult to read--let alone scan the barcode. Does anyone have any suggestions/hints on what they are using for their gram stain that may lessen the acetone removal of the print on the labels? Is there a system that will not affect the slide labels? Is there a gram stain that anyone could recommend that does not use acetone? Thank you in advance, Jacqueline Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From sgoebel <@t> xbiotech.com Tue Oct 26 16:09:21 2010 From: sgoebel <@t> xbiotech.com (sgoebel@xbiotech.com) Date: Tue Oct 26 16:09:26 2010 Subject: [Histonet] gram stain/label issues Message-ID: <20101026140920.9e2d9aa830e8449a2412eb1e4f2f067e.c539b72ccb.wbe@email04.secureserver.net> Maybe try handwriting with a pencil then putting the label on aft erwards. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. < Bldg 4 Sui Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] gram stain/label issues From: Jacqueline Farnsworth <[1]Jacqueline.Farnsworth@cls.ab.ca> Date: Tue, October 26, 2010 1:56 pm To: "[2]Histonet@lists.ut <[3]Histonet@lists.uts HI all, We are doing gram stains manually, and the acetone is removing some of the alone scan what they are usin removal of the print on affect the slide labels? Is the recommend that does not use acetone? Thank you in advance, Jacqueline Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intende information. An disclosure is strictly in error, please notify the original message. Thank you. _______________________________________________ Histonet mailing list [4]Histonet@lists.utsouth [5]http: References 1. 3D"mailto:Jacqueline.Farnsworth@cls 2. 3D"mailto:Histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"mailto:Histonet@lists.utsouthwestern.edu" 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From JMacDonald <@t> mtsac.edu Tue Oct 26 22:49:29 2010 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Oct 26 22:49:34 2010 Subject: [Histonet] xylene substitute Message-ID: We routinely use Richard-Allan Clear-Rite 3 with Permount. We have for years. Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From gu.lang <@t> gmx.at Wed Oct 27 02:19:29 2010 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 27 02:19:37 2010 Subject: [Histonet] pyrosequencing Message-ID: <977F094105EB441FBB1E0A72DB21D349@dielangs.at> Dear Histonetters! Is anyone with experiences on pyrosequencing kras out there? I would be happy to have someone I could share my problems with. ;-) Gudrun Lang Histolab Linz, Austria From kmerriam2003 <@t> yahoo.com Wed Oct 27 06:44:28 2010 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Oct 27 06:44:35 2010 Subject: [Histonet] cryojane problems In-Reply-To: <9FE33752FA3F3647BC85BCDC3EA6C3D73BD8A8@usctmx1176.merck.com> References: <983813.11449.qm@web50306.mail.re2.yahoo.com> <9FE33752FA3F3647BC85BCDC3EA6C3D73BD8A8@usctmx1176.merck.com> Message-ID: <564167.87801.qm@web50304.mail.re2.yahoo.com> Brett- I have been using?the 4X slides, but they don't seem to help.? I dont have this problem with the cartilage alone, but when there is bone attached, it is a nightmare. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Connolly, Brett M" To: Kim Merriam ; Histonet Sent: Tue, October 26, 2010 9:41:18 AM Subject: RE: [Histonet] cryojane problems Kim, I would recommend using their '4x' slides - they have more adhesive on them. I don't think flashing more than once has any benefit. Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, October 26, 2010 9:32 AM To: Histonet Subject: [Histonet] cryojane problems Hi All, I have been using the cryojane to cut human articular cartilage, with excellent results.? Now I need to cut frozen sections of rat articular cartilage that?contain some underlying bone.??I am getting nice sections, but when I?peel the tape off the slide, a bunch of my tissue is staying on the tape.? I?have tried increasing the number of flashes (to a whopping 10!), hoping that this will help adhere the sections, but it hasn't. Any tips for me? Kim? ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From theresec <@t> slonepartners.com Wed Oct 27 07:30:24 2010 From: theresec <@t> slonepartners.com (Therese Cook) Date: Wed Oct 27 07:30:31 2010 Subject: [Histonet] Be recognized for making a difference! Anatomic Pathology Manager position Message-ID: <0015A905-3E16-4220-A3EC-9C491FB6C8A0@slonepartners.com> Good Morning! Slone Partners seeks an Anatomic Pathology Manager for a fast-paced laboratory in a nationally recognized teaching hospital in the Chicago area. Three to five years of supervisory or management experience is required. The ideal candidate will have histology management experience in an academic medical center environment. Special features of this position. The hospital is looking for a strong leader who wants to be recognized for the difference he or she can make. It offers excellent benefits and a stable work environment, and encourages innovation, embraces diversity, and values human dignity. If you meet these qualifications and would like to be considered for this opportunity, please contact Therese Cook at theresec@slonepartners.com. Best Regards, Therese Cook MT (ASCP) SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX: 330.232.9333 www.slonepartners.com From WVanTilburg <@t> GENERAL-DATA.com Wed Oct 27 08:03:28 2010 From: WVanTilburg <@t> GENERAL-DATA.com (VanTilburg, Walt) Date: Wed Oct 27 08:01:50 2010 Subject: [Histonet] Histonet mailing list submissions Message-ID: To whom ever, Please let me know if this is acceptable. Our company is looking for a HT, MT or PA to represent our unique products in the western United States. This person will use their knowledge of APLAB workflow, verbal and written skills to help labs move to a more error proof specimen tracking system. This position will require 60% travel within the western states. To learn more about our products visit our web site Http://www.general-data.com/healthcare. If you are interested send your resume with salary requirements to me. Walt Walter Van Tilburg Director of Healthcare sales General Data Company, Inc. 26500 Bruce Road Bay Village, Ohio 44140 440-823-5495 - Mobile 440-808-8983 - Office 440-808-8995 - Fax WVanTilburg@general-data.com Visit our web site http://www.general-data.com/healthcare/ Walt Walter Van Tilburg Director of Healthcare sales General Data Company, Inc. 26500 Bruce Road Bay Village, Ohio 44140 440-823-5495 - Mobile 440-808-8983 - Office 440-808-8995 - Fax WVanTilburg@general-data.com Visit our web site http://www.general-data.com/healthcare/ ________________________________ This email may contain confidential General Data Company, Inc. information: any unauthorized or improper disclosure, copying, distribution or use of the contents of this email and attached document(s) is prohibited and may be a criminal offense. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you received this email in error, please reply immediately to the sender & delete this message and the attached document(s) without disclosure. From Sharon.Davis-Devine <@t> carle.com Wed Oct 27 08:37:28 2010 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Oct 27 08:37:33 2010 Subject: [Histonet] Telepathology for frozen sections Message-ID: We have several surgicenters which our pathologists must travel to perform frozen sections a couple of times a week. We are looking into a telepathology system in which a PA would travel to the surgicenter, prepare the specimen and transmit the image (live) to the pathologist in their office. The telepathology system allows the pathologist, using the computer keyboard, to manipulate the slide by moving it around, focusing, making a diagnosis and then communicating that diagnosis to the surgeon in the operating room. My question to all of you our their in Histoland is: are any of you out there using a system like this? And if you do, what CPT do you use to charge for it? Is this an acceptable standard of care for our patients, remaining in compliance with all of the regulartory agencies out there? I have heard of telepathology for review and diagnosis of surgical cases but not for frozen section. Any information or advise will be greatly appreciated. Thanks so much. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From jcampbell_mdl <@t> frontier.com Wed Oct 27 09:28:35 2010 From: jcampbell_mdl <@t> frontier.com (Jen Campbell) Date: Wed Oct 27 09:29:52 2010 Subject: [Histonet] (no subject) In-Reply-To: <518756125.794109.1288189647141.JavaMail.root@cl02-host03.roch.ny.frontiernet.net> Message-ID: <496880451.794132.1288189715774.JavaMail.root@cl02-host03.roch.ny.frontiernet.net> Good day, Our private dermpath lab, located in central/western NY is eager to fill an immediate opening for a full time histotech. A relocation bonus is negotiable. To learn more please contact me directly. Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 From Vickroy.Jim <@t> mhsil.com Wed Oct 27 10:32:46 2010 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Oct 27 10:32:56 2010 Subject: [Histonet] breast core fixation times Message-ID: <24A4826E8EF0964D86BC5317306F58A554FF78333E@mmc-mail.ad.mhsil.com> Question ( I know this gets discussed every week, however please humor me.) On breast tissue specimens the fixation time guidelines we have been using is 6 hours to 48 hours. We have exempted breast core biopsies in the past and stated that the fixation time can be less than 6 hours. I have asked my pathologists if we need to adhere to the 6 hr minimum with breast core biopsies. I also know that new guidelines including time before fixation is also coming soon. Can someone give me the "reader's digest version" of the guidelines on fixation times? Now and starting in Jan. 1, 2011. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Bill.Tench <@t> pph.org Wed Oct 27 10:50:11 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Oct 27 10:50:21 2010 Subject: [Histonet] breast fixation times Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5780@MAIL1.pph.local> There is no exception for core biopsies, as reasonable as that may seem. I have had that discussion with the purveyors of the guidelines. 6-48 is the current standard. there was a lot of discussion about exceeding 48 and using the FISH option. My colleague responsible for this wrote: It is in the CAP checklist, ANP 22998: If the laboratory assesses Her2 by IHC or Her2 gene amplification by in-situ hybridization (FISH, CISH, SISH), does the lab have a documented procedure for ensuring appropriate length of fixation of specimens tested? Specimens subject to Her2 testing should be fixed in 10% neutral buffered formalin for at least 6 hours and no longer than 48 hours. While fixation outside of these time limits is not an absolute exclusion criterion for Her2 testing, labs should qualify any negative results for specimens fixed less than 6 hours or longer than 48 hours. For cases with negative results by IHC, consideration should be given to performing confirmatory analysis by in-situ hybridization. There is also a table in the original ASCO/CAP Guideline Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol 131, Jan 2007) that states that tissue fixed in formalin for greater than 48 hours is not an absolute exclusion criterion, but if known to be fixed longer than 48 hours or unknown, the report should qualify any negative result with this information (table 6). As for upcoming changes, i don't know other than these time limitations are suppose to be more rigorously applied to ER and PR, along with the newly instituted documentation of time between excision and time placed in fixative. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From Melissa.Kuhnla <@t> chsli.org Wed Oct 27 11:20:52 2010 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Oct 27 11:21:10 2010 Subject: [Histonet] breast fixation times In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5780@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A5780@MAIL1.pph.local> Message-ID: CAP/ASCO released guidelines this summer for ER and PR. Fixation should be between 6 and 72 hours. Time before fixation should be less than one hour. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, October 27, 2010 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] breast fixation times There is no exception for core biopsies, as reasonable as that may seem. I have had that discussion with the purveyors of the guidelines. 6-48 is the current standard. there was a lot of discussion about exceeding 48 and using the FISH option. My colleague responsible for this wrote: It is in the CAP checklist, ANP 22998: If the laboratory assesses Her2 by IHC or Her2 gene amplification by in-situ hybridization (FISH, CISH, SISH), does the lab have a documented procedure for ensuring appropriate length of fixation of specimens tested? Specimens subject to Her2 testing should be fixed in 10% neutral buffered formalin for at least 6 hours and no longer than 48 hours. While fixation outside of these time limits is not an absolute exclusion criterion for Her2 testing, labs should qualify any negative results for specimens fixed less than 6 hours or longer than 48 hours. For cases with negative results by IHC, consideration should be given to performing confirmatory analysis by in-situ hybridization. There is also a table in the original ASCO/CAP Guideline Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol 131, Jan 2007) that states that tissue fixed in formalin for greater than 48 hours is not an absolute exclusion criterion, but if known to be fixed longer than 48 hours or unknown, the report should qualify any negative result with this information (table 6). As for upcoming changes, i don't know other than these time limitations are suppose to be more rigorously applied to ER and PR, along with the newly instituted documentation of time between excision and time placed in fixative. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From JWeems <@t> sjha.org Wed Oct 27 11:22:08 2010 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Oct 27 11:22:15 2010 Subject: [Histonet] RE: breast fixation times In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5780@MAIL1.pph.local> References: <2820431BF953BB4DA3E9E1A5882265FD034A5780@MAIL1.pph.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640448AF2A61@CHEXCMS10.one.ads.che.org> Thanks for this good explanation, Bill. One can not follow the guidelines and document the variant in the report, but not following them could hurt the patient if there is a clinical trial they might participate in. Clinical trials follow the protocol to the letter and if the FDA requirement is not met, the patient can not participate. The times were extended for ER and PR to 72 hours, but NOT yet for Her2. So...because the tissue is all the same, we must follow the 48 hour limit. We just had a case this weekend. Had the clinical staff remove it from the processor on Sun morning and embedded it Monday. We don't ususally have this problem as we are a 6-day lab, but it was finished too late on Fri. Cheers,j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, October 27, 2010 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] breast fixation times There is no exception for core biopsies, as reasonable as that may seem. I have had that discussion with the purveyors of the guidelines. 6-48 is the current standard. there was a lot of discussion about exceeding 48 and using the FISH option. My colleague responsible for this wrote: It is in the CAP checklist, ANP 22998: If the laboratory assesses Her2 by IHC or Her2 gene amplification by in-situ hybridization (FISH, CISH, SISH), does the lab have a documented procedure for ensuring appropriate length of fixation of specimens tested? Specimens subject to Her2 testing should be fixed in 10% neutral buffered formalin for at least 6 hours and no longer than 48 hours. While fixation outside of these time limits is not an absolute exclusion criterion for Her2 testing, labs should qualify any negative results for specimens fixed less than 6 hours or longer than 48 hours. For cases with negative results by IHC, consideration should be given to performing confirmatory analysis by in-situ hybridization. There is also a table in the original ASCO/CAP Guideline Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol 131, Jan 2007) that states that tissue fixed in formalin for greater than 48 hours is not an absolute exclusion criterion, but if known to be fixed longer than 48 hours or unknown, the report should qualify any negative result with this information (table 6). As for upcoming changes, i don't know other than these time limitations are suppose to be more rigorously applied to ER and PR, along with the newly instituted documentation of time between excision and time placed in fixative. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Bill.Tench <@t> pph.org Wed Oct 27 11:25:38 2010 From: Bill.Tench <@t> pph.org (Tench, Bill) Date: Wed Oct 27 11:25:48 2010 Subject: [Histonet] RE: breast fixation times In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640448AF2A61@CHEXCMS10.one.ads.che.org> References: <2820431BF953BB4DA3E9E1A5882265FD034A5780@MAIL1.pph.local> <92AD9B20A6C38C4587A9FEBE3A30E1640448AF2A61@CHEXCMS10.one.ads.che.org> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5783@MAIL1.pph.local> My apologies for not including the updates accurate for ER and PR. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Wednesday, October 27, 2010 9:22 AM To: Tench, Bill; histonet@lists.utsouthwestern.edu Subject: RE: breast fixation times Thanks for this good explanation, Bill. One can not follow the guidelines and document the variant in the report, but not following them could hurt the patient if there is a clinical trial they might participate in. Clinical trials follow the protocol to the letter and if the FDA requirement is not met, the patient can not participate. The times were extended for ER and PR to 72 hours, but NOT yet for Her2. So...because the tissue is all the same, we must follow the 48 hour limit. We just had a case this weekend. Had the clinical staff remove it from the processor on Sun morning and embedded it Monday. We don't ususally have this problem as we are a 6-day lab, but it was finished too late on Fri. Cheers,j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Wednesday, October 27, 2010 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] breast fixation times There is no exception for core biopsies, as reasonable as that may seem. I have had that discussion with the purveyors of the guidelines. 6-48 is the current standard. there was a lot of discussion about exceeding 48 and using the FISH option. My colleague responsible for this wrote: It is in the CAP checklist, ANP 22998: If the laboratory assesses Her2 by IHC or Her2 gene amplification by in-situ hybridization (FISH, CISH, SISH), does the lab have a documented procedure for ensuring appropriate length of fixation of specimens tested? Specimens subject to Her2 testing should be fixed in 10% neutral buffered formalin for at least 6 hours and no longer than 48 hours. While fixation outside of these time limits is not an absolute exclusion criterion for Her2 testing, labs should qualify any negative results for specimens fixed less than 6 hours or longer than 48 hours. For cases with negative results by IHC, consideration should be given to performing confirmatory analysis by in-situ hybridization. There is also a table in the original ASCO/CAP Guideline Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol 131, Jan 2007) that states that tissue fixed in formalin for greater than 48 hours is not an absolute exclusion criterion, but if known to be fixed longer than 48 hours or unknown, the report should qualify any negative result with this information (table 6). As for upcoming changes, i don't know other than these time limitations are suppose to be more rigorously applied to ER and PR, along with the newly instituted documentation of time between excision and time placed in fixative. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 Bill.Tench@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- From Audrey.Pagan <@t> nyumc.org Wed Oct 27 12:07:50 2010 From: Audrey.Pagan <@t> nyumc.org (Pagan, Audrey) Date: Wed Oct 27 12:07:59 2010 Subject: [Histonet] Histotechnicians and Lead Histotechnician Openings Message-ID: Hello Members, NYU Langone Medical Center currently has 3 openings in our Histology Lab in the Department of Anatomic Pathology. We are located on First Ave in Manhattan, NY. Please see position descriptions below. Thanks, Lead Histotechnician Position Summary: Prepares tissue specimens for pathological examination. Assists in the supervision and training of histology technicians in the anatomic pathology laboratory. Scope of services includes: histology, immunohistochemistry, renal, & immunoflourescence. Minimum Qualifications: Bachelor's degree in the major of biology, chemistry or physical sciences from an accredited college or university. License as a Clinical Laboratory Technician by the State of New York. Minimum 4 years of experience in a clinical laboratory required. Effective oral, written, communication and interpersonal skills. Demonstrates above average knowledge or expertise in dealing with scientific principals involved in the methodology performed; as well as alternative methodologies and their application to a given situation. Preferred Qualifications: ASCP - HTL Certificate. Excellent computer knowledge using Microsoft word, excel, and outlook. Position Hours: 2:00 PM - 10:00 PM Interested: Please email resume to Audrey Pagan Audrey.Pagan@nyumc.org POSTED: 10/27/10 Histotechnician (2 postitions) Position Summary: Prepares tissue specimens for pathological examination. Scope of services includes: histology, immunohistochemistry, renal, & immunoflourescence. Minimum Qualifications: License as a Clinical Laboratory Technician by the State of New York. Displays competency in embedding, microtomy, routine, special stain and IHC/ISH staining techniques for clinical tissue samples. Preferred Qualifications: ASCP - HT Certificate. 1 year of experience in a clinical laboratory. Computer literacy using Microsoft word, excel, and outlook. Position Hours: 10:00AM - 6:00 PM and 1:00PM - 9:00PM Interested: Please email resume to Audrey Pagan Audrey.Pagan@nyumc.org POSTED: 10/27/10 ------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
From TJJ <@t> stowers.org  Wed Oct 27 12:10:35 2010
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Wed Oct 27 12:10:42 2010
Subject: [Histonet] Re: xylene substitute
Message-ID: 

Hi all,

I just wanted to let folks know that if you plan to switch to a xylene substitute, realize that the substitutes are more intolerant of water than xylene is. Your last alcohol will have to be as anhydrous as you can get it or you will have water carryover that may cause some problems. If you live in a humid climate, this might end up being problematic for you.

Having said that, we prefer using ProPar or Clear-Rite 3 (aliphatic hydrocarbon reagents) over the limonenes.

Carry on!

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO


From akemiat3377 <@t> yahoo.com  Wed Oct 27 13:07:07 2010
From: akemiat3377 <@t> yahoo.com (Akemi Allison)
Date: Wed Oct 27 13:07:19 2010
Subject: [Histonet] Thanks interfacing the IHC bond and Cassettelabelers to
	Co-Path 
Message-ID: 

Hi Histonet Land,

I just wanted to drop you a line and thank everyone that sent me  
direct and indirect information regarding my question "interfacing  
the IHC bond and Cassettelabelers to Co-Path"  I forwarded the  
information to the administrator that had proposed the question.  She  
was able to prose her information to the proper channels to make her  
case for justification.

Thank you again!

Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377@yahoo.com

From JMacDonald <@t> mtsac.edu  Wed Oct 27 13:22:29 2010
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Wed Oct 27 13:22:37 2010
Subject: [Histonet] Re: xylene substitute
In-Reply-To: 
Message-ID: 

xylene substitutes also require more time than xylene





"Johnson, Teri"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
10/27/2010 10:13 AM

To
"'histonet@lists.utsouthwestern.edu'" 
cc

Subject
[Histonet] Re: xylene substitute






Hi all,

I just wanted to let folks know that if you plan to switch to a xylene 
substitute, realize that the substitutes are more intolerant of water than 
xylene is. Your last alcohol will have to be as anhydrous as you can get 
it or you will have water carryover that may cause some problems. If you 
live in a humid climate, this might end up being problematic for you.

Having said that, we prefer using ProPar or Clear-Rite 3 (aliphatic 
hydrocarbon reagents) over the limonenes.

Carry on!

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From lbustamante <@t> cvm.tamu.edu  Wed Oct 27 15:19:36 2010
From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante)
Date: Wed Oct 27 15:20:17 2010
Subject: [Histonet] Absolute with methanol
Message-ID: <4CC84309020000B9000E3D5E@CVM.TAMU.EDU>

Does anyone knows if there is any problem using Absolute ETOH with
Methanol instead Absolute alone when it comes to make any staining
solutions?
Thank you.
Lin.

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas A&M University
College Station, TX 77843-4458
From lbustamante <@t> cvm.tamu.edu  Wed Oct 27 15:21:37 2010
From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante)
Date: Wed Oct 27 15:22:09 2010
Subject: [Histonet] Parafolicular cells, "C" cells
Message-ID: <4CC84381020000B9000E3D6A@CVM.TAMU.EDU>

Do you know a good specific special stain (not IHC stain) for this cells
of the thyroid gland?

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas A&M University
College Station, TX 77843-4458
From lbustamante <@t> cvm.tamu.edu  Wed Oct 27 15:23:13 2010
From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante)
Date: Wed Oct 27 15:23:53 2010
Subject: [Histonet] GMA - 100 um sections
Message-ID: <4CC843E1020000B9000E3D73@CVM.TAMU.EDU>

Anyone have done this? Please let us know what you did.
Thank you.
Lin.

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas A&M University
College Station, TX 77843-4458
From mucram11 <@t> comcast.net  Wed Oct 27 15:46:44 2010
From: mucram11 <@t> comcast.net (Pamela Marcum)
Date: Wed Oct 27 15:46:48 2010
Subject: [Histonet] Absolute with methanol
In-Reply-To: <4CC84309020000B9000E3D5E@CVM.TAMU.EDU>
Message-ID: <1886227106.59430.1288212404358.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>



It should not be a problem as most reagent alcohol contains both methanol and isopropanol at roughly 90% ETOH with 5% of each of the others.? This way the alcohol is not taxed as it is unfit for human consumption according to the ATF.? The goal was to make an alcohol?we could afford to buy in the for profit sector that met the non-consumption rule, since it is poison, so to speak. 



Pam Marcum 

UAMS 


----- Original Message ----- 
From: "Lin Bustamante"  
To: histonet@lists.utsouthwestern.edu 
Sent: Wednesday, October 27, 2010 3:19:36 PM 
Subject: [Histonet] Absolute with methanol 

Does anyone knows if there is any problem using Absolute ETOH with 
Methanol instead Absolute alone when it comes to make any staining 
solutions? 
Thank you. 
Lin. 

Lin S. Bustamante, B.Sc.; HT(ASCP) 
Research Associate 
Histology Lab Supervisor 
Veterinary Integrative Bioscience 
Texas A&M University 
College Station, TX 77843-4458 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



----- Original Message ----- 
From: "Lin Bustamante"  
To: histonet@lists.utsouthwestern.edu 
Sent: Wednesday, October 27, 2010 3:19:36 PM 
Subject: [Histonet] Absolute with methanol 

Does anyone knows if there is any problem using Absolute ETOH with 
Methanol instead Absolute alone when it comes to make any staining 
solutions? 
Thank you. 
Lin. 

Lin S. Bustamante, B.Sc.; HT(ASCP) 
Research Associate 
Histology Lab Supervisor 
Veterinary Integrative Bioscience 
Texas A&M University 
College Station, TX 77843-4458 
_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
From historsd <@t> aol.com  Wed Oct 27 20:44:07 2010
From: historsd <@t> aol.com (historsd@aol.com)
Date: Wed Oct 27 20:40:04 2010
Subject: [Histonet] lever vs handwheel?
Message-ID: <8CD4458BDAF5082-F0C-75ED@webmail-m034.sysops.aol.com>

Can someone tell me the difference between a handwheel driven cryostat and a 'lever' driven cryostat?


From mpence <@t> grhs.net  Thu Oct 28 08:12:51 2010
From: mpence <@t> grhs.net (Mike Pence)
Date: Thu Oct 28 08:12:57 2010
Subject: [Histonet] Finesse ME+
Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974A6F@is-e2k3.grhs.net>

Hey Everyone,
I feel like a nut just asking this question, but I do not have an
answer.  We have a Finesse ME+ microtome that the techs use in automatic
mode 95% of the time. They will however, hand trim blocks sometimes that
may be difficult to cut. I have 1 tech that turns the hand wheel
counterclockwise to face the block then turns it clockwise to cut.  Does
anyone know if this can affect the inter workings of the microtome?
 
Mike
From candice_camille <@t> yahoo.com  Thu Oct 28 08:48:09 2010
From: candice_camille <@t> yahoo.com (Candice Smoots)
Date: Thu Oct 28 08:48:12 2010
Subject: Fw: [Histonet] cryojane problems
In-Reply-To: <9FE33752FA3F3647BC85BCDC3EA6C3D73BD8A8@usctmx1176.merck.com>
References: <983813.11449.qm@web50306.mail.re2.yahoo.com>
	<9FE33752FA3F3647BC85BCDC3EA6C3D73BD8A8@usctmx1176.merck.com>
Message-ID: <177573.11999.qm@web62203.mail.re1.yahoo.com>



 I remain yours truely, 

Candice Camille 



----- Forwarded Message ----
From: Candice Smoots 
To: "Connolly, Brett M" 
Cc: histonet 
Sent: Thu, October 28, 2010 8:43:52 AM
Subject: Re: [Histonet] cryojane problems


Hi Guys

I am new to histology. Could you please explain what are flashes?  Thanks

 I remain yours truely, 

Candice Camille 




________________________________
From: "Connolly, Brett M" 
To: Kim Merriam ; Histonet 

Sent: Tue, October 26, 2010 8:41:18 AM
Subject: RE: [Histonet] cryojane problems

Kim,

I would recommend using their '4x' slides - they have more adhesive on them.

I don't think flashing more than once has any benefit.

Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly@merck.com
T- 215-652-2501
F- 215-993-6803




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Tuesday, October 26, 2010 9:32 AM
To: Histonet
Subject: [Histonet] cryojane  problems

Hi All,

I have been using the cryojane to cut human articular cartilage, with excellent 
results.  Now I need to cut frozen sections of rat articular cartilage 
that contain some underlying bone.  I am getting nice sections, but when I peel 
the tape off the slide, a bunch of my tissue is staying on the tape.  I have 
tried increasing the number of flashes (to a whopping 10!), hoping that this 
will help adhere the sections, but it hasn't.

Any tips for me?

Kim 
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 


      
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your system.


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Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From mcauliff <@t> umdnj.edu  Thu Oct 28 09:20:45 2010
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Thu Oct 28 09:17:58 2010
Subject: [Histonet] Parafolicular cells, "C" cells
In-Reply-To: <4CC84381020000B9000E3D6A@CVM.TAMU.EDU>
References: <4CC84381020000B9000E3D6A@CVM.TAMU.EDU>
Message-ID: <4CC986BD.1080500@umdnj.edu>

Lead hematoxylin.
See Solcia et al. Histochemie 20:116-126, 1969
or google lead hematoxylin and see what pops up.

Geoff

Lin Bustamante wrote:
> Do you know a good specific special stain (not IHC stain) for this cells
> of the thyroid gland?
>
> Lin S. Bustamante, B.Sc.; HT(ASCP)
> Research Associate
> Histology Lab Supervisor
> Veterinary Integrative Bioscience
> Texas A&M University
> College Station, TX 77843-4458
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



From eroy <@t> illinois.edu  Tue Oct 19 10:26:50 2010
From: eroy <@t> illinois.edu (Ed Roy)
Date: Thu Oct 28 09:20:36 2010
Subject: [Histonet] lymph nodes peeling out of OCT
Message-ID: <4CBDB8BA.9090205@illinois.edu>

  Hi
We do a lot of IHC on mouse lymph nodes, unfixed, fresh-frozen in OCT. 
Sometimes individual lymph nodes will peel away from the OCT as they are 
being cut, and then curl up, making them impossible to get a good 
section.  In the same mold several will be fine and one will curl up.  
Cutting further on the same block, the problem lymph node may be fine.  
Any ideas on what variables determine this, either in the initial 
freezing, or in the cutting? Would it help to soak the tissue at room 
temp in the OCT for a period of time before freezing? Dip in PBS before 
freezing?
Thanks for any insights.
Ed

-- 

Edward Roy, PhD
Professor of Pathology
Professor of Molecular and Integrative Physiology
University of Illinois at Urbana-Champaign
506 S. Mathews Ave.
Urbana, IL 61801
217 333-3375


From eroy <@t> illinois.edu  Thu Oct 28 09:38:23 2010
From: eroy <@t> illinois.edu (Ed Roy)
Date: Thu Oct 28 09:38:27 2010
Subject: [Histonet] lymph nodes peeling out of OCT
Message-ID: <4CC98ADF.3010907@illinois.edu>

Hi
We do a lot of IHC on mouse lymph nodes, unfixed, fresh-frozen in OCT. 
Sometimes individual lymph nodes will peel away from the OCT as they are 
being cut, and then curl up, making it impossible to get a good 
section.  In the same mold several will be fine and one will curl up.  
Cutting further on the same block, the problem lymph node may be fine.  
Any ideas on what variables determine this, either in the initial 
freezing, or in the cutting? Would it help to soak the tissue at room 
temp in the OCT for a period of time before freezing? Dip in PBS before 
freezing?
Thanks for any insights.
Ed

-- 

Edward Roy, PhD
Professor of Pathology
Professor of Molecular and Integrative Physiology
University of Illinois at Urbana-Champaign
506 S. Mathews Ave.
Urbana, IL 61801
217 333-3375


From alyssa <@t> alliedsearchpartners.com  Thu Oct 28 10:25:24 2010
From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson)
Date: Thu Oct 28 10:25:29 2010
Subject: [Histonet] Histology Technician Needed-Long Island, NY
Message-ID: 

Allied Search Partners has been retained to prescreen and search for a
qualified Histology Technician candidate in New York, NY.



Location: On Long Island, near Hicksville, NY



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   of a total of about 24 doctors.



   - Private Laboratory and Team Oriented/Family Oriented Environment



   - No Quotas! Have the flexibility to get your work done on your own time



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   - High Volume Laboratory





Requirements:



   - Ability to work with little or no supervision
   - NY licensed
   - ASCP certified
   - Meet the CLIA eligibility to gross



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   - Salary is dependent upon experience and is competitive to current
   market rate
   - Benefits include paid time off, 401k, life, health, long term
   disability insurance, additional days off for all Jewish holidays (8-15 days
   a year)



To apply:



Please first submit resume to Alyssa@alliedsearchpartners.com   At that time
we will review your resume with our team of recruiters and if all
requirements are met will schedule a phone screen with you to discuss
further questions and concerns.


-- 
Alyssa Peterson, Director of Candidate Recruitment
LinkedIN:http://www.linkedin.com/in/alyssapetersonasp

Allied Search Partners

T: 888.388.7571

F: 888.388.7572

www.alliedsearchpartners.com

This email including its attachments is intended only for the confidential
use of the individual to whom it is addressed. If you are not the intended
recipient, any use, dissemination, distribution or copying of this message
or its attachments is prohibited.  If you have received this message in
error, please notify us immediately, and delete this message and its
attachments permanently from your system.
From sbaldwin <@t> mhhcc.org  Thu Oct 28 12:03:04 2010
From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org)
Date: Thu Oct 28 12:04:35 2010
Subject: [Histonet] BLOCK PRICE
Message-ID: 

HISTONETTERS
Does anyone know what a fair price for a block, cut, stained and coverslipped would be??

Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-482-0210, 482-0216,  Fax 812-482-0232, 
Pager 812-481-0897
Confidential information, Authorized use only.
From kenneth.a.troutman <@t> Vanderbilt.Edu  Thu Oct 28 13:21:08 2010
From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A)
Date: Thu Oct 28 13:21:28 2010
Subject: [Histonet] Parafolicular cells, "C" cells
Message-ID: <7B310892042DA74CB3590053F424CFE61381DA02D2@ITS-HCWNEM06.ds.Vanderbilt.edu>

Hi Lin,

I am not aware of a special stain that is that specific for C-cells, but a calcitonin immunostain is pretty specific.

Good luck

Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN  37232

Message: 6
Date: Wed, 27 Oct 2010 15:21:37 -0500
From: "Lin Bustamante" 
Subject: [Histonet] Parafolicular cells, "C" cells
To: 
Message-ID: <4CC84381020000B9000E3D6A@CVM.TAMU.EDU>
Content-Type: text/plain; charset=US-ASCII

Do you know a good specific special stain (not IHC stain) for this cells
of the thyroid gland?

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas A&M University
College Station, TX 77843-4458


From laurie.colbert <@t> huntingtonhospital.com  Thu Oct 28 13:51:59 2010
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Thu Oct 28 13:52:05 2010
Subject: [Histonet] Nitroblue Tetrazolium Chloride
Message-ID: <57BE698966D5C54EAE8612E8941D768309D02881@EXCHANGE3.huntingtonhospital.com>

I previously posted a question regarding Nitroblue Tetrazolium Chloride,
but I didn't really receive the info that I needed - so I thought I
would ask again.

 

I need to purchase this item for a research doc.  He wants to immerse
tissue in this solution for 24 hours and then process as usual.  It is
my understanding that this is some kind of dye.  I see that I can order
it from Sigma in tablet form.  I've seen it from other companies in a
powder form.  

 

Has anyone ever used this reagent in the capacity that I describe?  Is
it available as a ready-to-use solution/liquid?  Is there a certain
strength/percentage that I should use?

 

Thanks,

Laurie Colbert

From sfeher <@t> CMC-NH.ORG  Thu Oct 28 16:50:11 2010
From: sfeher <@t> CMC-NH.ORG (Feher, Stephen)
Date: Thu Oct 28 16:50:15 2010
Subject: [Histonet] RE: breast fixation times
In-Reply-To: <2820431BF953BB4DA3E9E1A5882265FD034A5783@MAIL1.pph.local>
References: <2820431BF953BB4DA3E9E1A5882265FD034A5780@MAIL1.pph.local><92AD9B20A6C38C4587A9FEBE3A30E1640448AF2A61@CHEXCMS10.one.ads.che.org>
	<2820431BF953BB4DA3E9E1A5882265FD034A5783@MAIL1.pph.local>
Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC2426C7E4@exchange.cmc-nh.org>

Great discussion, comprehensive yet concise.  Thanks Bill and Joyce and
Melissa. 


Steve

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Wednesday, October 27, 2010 12:26 PM
To: Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: breast fixation times

My apologies for not including the updates accurate for ER and PR. 


Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

-----Original Message-----
From: Weems, Joyce [mailto:JWeems@sjha.org]
Sent: Wednesday, October 27, 2010 9:22 AM
To: Tench, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: breast fixation times

Thanks for this good explanation, Bill.

One can not follow the guidelines and document the variant in the
report, but not following them could hurt the patient if there is a
clinical trial they might participate in. Clinical trials follow the
protocol to the letter and if the FDA requirement is not met, the
patient can not participate. 

The times were extended for ER and PR to 72 hours, but NOT yet for Her2.
So...because the tissue is all the same, we must follow the 48 hour
limit. We just had a case this weekend. Had the clinical staff remove it
from the processor on Sun morning and embedded it Monday. We don't
ususally have this problem as we are a 6-day lab, but it was finished
too late on Fri. 

Cheers,j

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax 



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Wednesday, October 27, 2010 11:50
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] breast fixation times

There is no exception for core biopsies, as reasonable as that may seem.
I have had that discussion with the purveyors of the guidelines.  6-48
is the current standard.  there was a lot of discussion about exceeding
48 and using the FISH option.  My colleague responsible for this wrote:
It is in the CAP checklist, ANP 22998:

If the laboratory assesses Her2 by IHC or Her2 gene amplification by
in-situ hybridization (FISH, CISH, SISH), does the lab have a documented
procedure for ensuring appropriate length of fixation of specimens
tested? 

Specimens subject to Her2 testing should be fixed in 10% neutral
buffered formalin for at least 6 hours and no longer than 48 hours.
While fixation outside of these time limits is not an absolute exclusion
criterion for Her2 testing, labs should qualify any negative results for
specimens fixed less than 6 hours or longer than 48 hours. For cases
with negative results by IHC, consideration should be given to
performing confirmatory analysis by in-situ hybridization. 

 There is also a table in the original ASCO/CAP Guideline
Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol 131,
Jan 2007) that states that tissue fixed in formalin for greater than 48
hours is not an absolute exclusion criterion, but if known to be fixed
longer than 48 hours or unknown, the report should qualify any negative
result with this information (table 6). 

As for upcoming changes, i don't know other than these time limitations
are suppose to be more rigorously applied to ER and PR, along with the
newly instituted documentation of time between excision and time placed
in fixative.
 
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

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From bouchalr <@t> rcbhsc.wvu.edu  Thu Oct 28 16:16:29 2010
From: bouchalr <@t> rcbhsc.wvu.edu (Bouchal, Rena L)
Date: Thu Oct 28 16:59:11 2010
Subject: [Histonet] Technical Specialist- Histology
Message-ID: 

Unique job opportunity in West Virginia college town.

West Virginia University Hospital is looking for a Technical
Specialist/Supervisor for the Histopathology Department.  B.A. or B.S.,
and HTL required. QIHC or QIHC-eligible mandatory.  The Histology
Supervisor is responsible for day-to-day technical supervision of
Histology, the Gross Room, the Morgue, Immunohistochemistry and Special
Stains. WVUH is a teaching hospital and includes interactions with the
Medical School, Residents, Histotechnology School, and Pathology
Assistant school. Salary commensurate with experience.

 Our large University provides many opportunities, including
nationally-ranked athletic teams, and arts and cultural events. The
rural area provides a safe, outdoors-type of environment with unique
beauty and activities. Check us out at  www.wvuh.com
 , or phone 304-293-7765.

 

 Anatomic Pathology Manager

West Virginia University Hospitals

304-293-7765

 

 

 

 



-----------------------------------------
Confidentiality Notice: This e-mail message, including any
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may contain confidential and privileged information.  Any
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 If you are not the intended recipient, please contact the sender
by reply e-mail and destroy all copies of the original message.
From louise.renton <@t> gmail.com  Fri Oct 29 02:04:28 2010
From: louise.renton <@t> gmail.com (louise renton)
Date: Fri Oct 29 02:04:33 2010
Subject: [Histonet] Nitroblue Tetrazolium Chloride
In-Reply-To: <57BE698966D5C54EAE8612E8941D768309D02881@EXCHANGE3.huntingtonhospital.com>
References: <57BE698966D5C54EAE8612E8941D768309D02881@EXCHANGE3.huntingtonhospital.com>
Message-ID: 

I used to do a "stain" on post mortem heart slices to look for recent
infarctions. the entire tissue turned a sort of bluey colour which remained
through formain fixation and processing. the tissue however was incubated in
 solution of NBT in a sodium cyanide containing buffer - not sure if that
would still be allowed these days - but if you want - i could send you the
protocol we used

best regards

On Thu, Oct 28, 2010 at 8:51 PM, Laurie Colbert <
laurie.colbert@huntingtonhospital.com> wrote:

> I previously posted a question regarding Nitroblue Tetrazolium Chloride,
> but I didn't really receive the info that I needed - so I thought I
> would ask again.
>
>
>
> I need to purchase this item for a research doc.  He wants to immerse
> tissue in this solution for 24 hours and then process as usual.  It is
> my understanding that this is some kind of dye.  I see that I can order
> it from Sigma in tablet form.  I've seen it from other companies in a
> powder form.
>
>
>
> Has anyone ever used this reagent in the capacity that I describe?  Is
> it available as a ready-to-use solution/liquid?  Is there a certain
> strength/percentage that I should use?
>
>
>
> Thanks,
>
> Laurie Colbert
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
From Melissa.Kuhnla <@t> chsli.org  Fri Oct 29 04:50:30 2010
From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa)
Date: Fri Oct 29 04:51:02 2010
Subject: [Histonet] RE: breast fixation times
In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC2426C7E4@exchange.cmc-nh.org>
References: <2820431BF953BB4DA3E9E1A5882265FD034A5780@MAIL1.pph.local><92AD9B20A6C38C4587A9FEBE3A30E1640448AF2A61@CHEXCMS10.one.ads.che.org><2820431BF953BB4DA3E9E1A5882265FD034A5783@MAIL1.pph.local>
	<0908FC0A43B87A4FB60EDCCA06AABC2426C7E4@exchange.cmc-nh.org>
Message-ID: 

One more question regarding ER/PR/Her2..who am I kidding, we will be
talking about them forever!!  With regards to the ER/PR publication: The
PR clone (Ventana 1E2) that we currently use is not listed as
'acceptable'. Is anyone else in this situation?  Are you switching to
another clone?? Have you come across anything speaking of this scenario?
What to do if you currently run a clone not mentioned?  It will be a
relatively easy validation for me, but I can't image I am alone here.
Thanks, Melissa

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher,
Stephen
Sent: Thursday, October 28, 2010 5:50 PM
To: Tench, Bill; Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: breast fixation times

Great discussion, comprehensive yet concise.  Thanks Bill and Joyce and
Melissa. 


Steve

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Wednesday, October 27, 2010 12:26 PM
To: Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: breast fixation times

My apologies for not including the updates accurate for ER and PR. 


Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

-----Original Message-----
From: Weems, Joyce [mailto:JWeems@sjha.org]
Sent: Wednesday, October 27, 2010 9:22 AM
To: Tench, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: breast fixation times

Thanks for this good explanation, Bill.

One can not follow the guidelines and document the variant in the
report, but not following them could hurt the patient if there is a
clinical trial they might participate in. Clinical trials follow the
protocol to the letter and if the FDA requirement is not met, the
patient can not participate. 

The times were extended for ER and PR to 72 hours, but NOT yet for Her2.
So...because the tissue is all the same, we must follow the 48 hour
limit. We just had a case this weekend. Had the clinical staff remove it
from the processor on Sun morning and embedded it Monday. We don't
ususally have this problem as we are a 6-day lab, but it was finished
too late on Fri. 

Cheers,j

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax 



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Wednesday, October 27, 2010 11:50
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] breast fixation times

There is no exception for core biopsies, as reasonable as that may seem.
I have had that discussion with the purveyors of the guidelines.  6-48
is the current standard.  there was a lot of discussion about exceeding
48 and using the FISH option.  My colleague responsible for this wrote:
It is in the CAP checklist, ANP 22998:

If the laboratory assesses Her2 by IHC or Her2 gene amplification by
in-situ hybridization (FISH, CISH, SISH), does the lab have a documented
procedure for ensuring appropriate length of fixation of specimens
tested? 

Specimens subject to Her2 testing should be fixed in 10% neutral
buffered formalin for at least 6 hours and no longer than 48 hours.
While fixation outside of these time limits is not an absolute exclusion
criterion for Her2 testing, labs should qualify any negative results for
specimens fixed less than 6 hours or longer than 48 hours. For cases
with negative results by IHC, consideration should be given to
performing confirmatory analysis by in-situ hybridization. 

 There is also a table in the original ASCO/CAP Guideline
Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol 131,
Jan 2007) that states that tissue fixed in formalin for greater than 48
hours is not an absolute exclusion criterion, but if known to be fixed
longer than 48 hours or unknown, the report should qualify any negative
result with this information (table 6). 

As for upcoming changes, i don't know other than these time limitations
are suppose to be more rigorously applied to ER and PR, along with the
newly instituted documentation of time between excision and time placed
in fixative.
 
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

[None] made the following annotations
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---------------------------------------------------------------------

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It may contain information that is privileged and confidential.  Any
unauthorized review, use, disclosure, or distribution is prohibited. If
you are not the intended recipient, please delete this message, and
reply to the sender regarding the error in a separate email. 
 


[None] made the following annotations
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From irena.kirbis <@t> hotmail.com  Fri Oct 29 08:14:29 2010
From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS)
Date: Fri Oct 29 08:14:35 2010
Subject: [Histonet] RE: Saponin Technique
In-Reply-To: 
References: <5680DA93771F0C48954CC8D38425E72401AB3516@ISMAIL.parrishmed.local>,
	
Message-ID: 


for removing  erythrocytes from hemorrhagic cytology samples we use filtration of bloody suspension through membrane filter with pore size 20 mikrons, you can find an article in Cytopathology. 2007 Jun;18(3):175-9. Epub 2007 Mar 27.
it is worth trying!
regards
Irena
 
> From: TMcNemar@lmhealth.org
> To: Valerie.Hannen@parrishmed.com; histonet@lists.utsouthwestern.edu
> Date: Mon, 25 Oct 2010 11:52:31 -0400
> CC: 
> Subject: [Histonet] RE: Saponin Technique
> 
> I don't know about the procedure you have but ours only uses a 1% solution of saponin, a 3% solution of calcium gluconate, and a balanced salt solution. We get the balanced salt solution from our pharmacy.
> 
> 
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar@lmhealth.org
> www.LMHealth.org
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
> Sent: Monday, October 25, 2010 11:40 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] FW: Saponin Technique
> 
> 
> 
> ________________________________
> 
> From: Hannen, Valerie
> Sent: Monday, October 25, 2010 11:39 AM
> To: 'histonet@list.utsouthwestern.edu'
> Subject: Saponin Technique
> 
> 
> Hi folks..
> I am hoping someone out there in Histo-land can help. One of our
> Pathologists is asking us to check into doing the Saponin Technique to
> lyse the red cells in some of our cytology specimens. She gave a copy of
> one of the pages from one of her manuals, and it shows that in order for
> us to do this we would need to buy alot of reagents that we currently do
> not have. Being that we would probably not be doing this technique very
> often, and not sure how cost effective it will be for us to buy the
> reagents, we are wondering if anyone knows of a commercial product that
> we can buy that would be a cheaper way out.
> 
> Thanks in advance!!
> Valerie Hannen, Histotechnologist
> Parrish Medical Center
> Titusville,Florida
> 
> 
> **************************************************************
> "This email is intended solely for the use of the individual to
> whom it is addressed and may contain information that is
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
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From mhale <@t> carisls.com  Fri Oct 29 08:54:30 2010
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Fri Oct 29 08:54:36 2010
Subject: [Histonet] Nashville HT Position 
Message-ID: <6F33D8418806044682A391273399860F05C38C06@s-irv-ex301.PathologyPartners.intranet>

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mhale@carisls.com

 

 

 

 

*** Please no recruiter's or vendors , HT applicant's only , thank you .


 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mhale@carisls.com 

 

 

 

 

 

 

From Ronald.Houston <@t> nationwidechildrens.org  Fri Oct 29 09:27:54 2010
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Fri Oct 29 09:28:04 2010
Subject: [Histonet] RE: Nitroblue Tetrazolium Chloride
In-Reply-To: <57BE698966D5C54EAE8612E8941D768309D02881@EXCHANGE3.huntingtonhospital.com>
References: <57BE698966D5C54EAE8612E8941D768309D02881@EXCHANGE3.huntingtonhospital.com>
Message-ID: 

Nitro BT is a tetrazolium salt used in many dehydrogenase enzyme histochemical techniques. It is used in powder form (I would recommend the Nitrotetrazolium Blue chloride from Sigma, cat N6876).



The Nitro BT test is used for the demonstration of early myocardial infarction in gross hearts at necropsy by a dehydrogenase enzyme histochemical reaction on slices on unfixed heart.

In brief, a redox indicator is reduced by mitochondrial enzymes in cardiac muscle cells producing a blue staining reaction in all but infarcted areas. The loss of enzyme activity from the tissue defines the damaged heart muscle



Incubation Medium:



      Nitro BT                            50mg

      0.2M Tris-HCl buffer, pH 7.4        100ml

      Sodium cyanide                      50mg

      ?-NAD                               100mg

   Adjust pH to 7.1

The stock solution can be kept for several weeks at 4C



Protocol:

1.                Rinse slices of myocardium in cold running water to remove the blood.

2.                Incubate slices at 37C for 30 minutes

3.                Wash in running water

4.                Store in formol saline


Result:
      Normal myocardium       - dark blue
Areas of infarcted muscle show diminished staining, the degree of which depends on age of infarct

Notes:
Loss of staining can be detected as early as 1-5 hr after clinically determined onset of infarct. Material can be tested for 3 days after death and even longer if stored at 4C

Cyanide is added to the incubation medium to bind keto acids, produced during the dehydrogenation of some hydroxy acids, which may inhibit the dehydrogenase reaction.

NAD is added to the reaction medium as it is lost from the tissue rapidly post-mortem, and greatly improves the sensitivity of the reaction.

Some reaction medium call for the addition of the electron transfer mediator phenazine methosulfate (PMS), ostensibly to increase the reaction velocity of the enzyme reaction. This is used to by-pass the reaction of tetrazolium reductases, mediating between the reduced coenzymes and the tetrazolium salt. However, if PMS is used, any active cytochrome oxidase must be inhibited, as it can either oxidize the PMS reducing the amount of formazan deposited at the site of the enzyme reaction or even reverse the pattern producing false non-selective staining and obscuring areas of recent myocardial damage.


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Thursday, October 28, 2010 2:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nitroblue Tetrazolium Chloride



I previously posted a question regarding Nitroblue Tetrazolium Chloride,

but I didn't really receive the info that I needed - so I thought I

would ask again.







I need to purchase this item for a research doc.  He wants to immerse

tissue in this solution for 24 hours and then process as usual.  It is

my understanding that this is some kind of dye.  I see that I can order

it from Sigma in tablet form.  I've seen it from other companies in a

powder form.







Has anyone ever used this reagent in the capacity that I describe?  Is

it available as a ready-to-use solution/liquid?  Is there a certain

strength/percentage that I should use?







Thanks,



Laurie Colbert



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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From PMonfils <@t> Lifespan.org  Fri Oct 29 12:13:44 2010
From: PMonfils <@t> Lifespan.org (Monfils, Paul)
Date: Fri Oct 29 12:14:03 2010
Subject: [Histonet] lymph nodes peeling out of OCT
In-Reply-To: <4CC98ADF.3010907@illinois.edu>
References: <4CC98ADF.3010907@illinois.edu>
Message-ID: <4EBFF65383B74D49995298C4976D1D5E075E08F9@LSRIEXCH1.lsmaster.lifespan.org>

I place all tissues in liquid OCT for at least 15 minutes before
freezing.  This virtually eliminates the problem of tissue sections
separating from the OCT after sectioning.



From ttruscot <@t> vetmed.wsu.edu  Fri Oct 29 12:55:45 2010
From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom)
Date: Fri Oct 29 12:55:35 2010
Subject: [Histonet] PLP fixative
Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB4011B20F1B562@CVMMBX.vetmed.wsu.edu>

I am seeking basic information on PLP fixative. What is it? Can I make it or buy it? How long does fixation take for lymph node? Thanks in advance, Tom Truscott
From micropathlabs <@t> yahoo.com  Fri Oct 29 12:56:14 2010
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Fri Oct 29 12:56:18 2010
Subject: [Histonet] Precipitating Silver
Message-ID: <450265.9811.qm@web57802.mail.re3.yahoo.com>

Has anyone heard of precipitating silver out of a solution by adding table salt? 
I read this somewhere as a means of disposing of silver easily. Any info would 
be appreciated.
Thanks,
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.


      
From PMonfils <@t> Lifespan.org  Fri Oct 29 13:02:26 2010
From: PMonfils <@t> Lifespan.org (Monfils, Paul)
Date: Fri Oct 29 13:02:43 2010
Subject: [Histonet] Precipitating Silver
In-Reply-To: <450265.9811.qm@web57802.mail.re3.yahoo.com>
References: <450265.9811.qm@web57802.mail.re3.yahoo.com>
Message-ID: <4EBFF65383B74D49995298C4976D1D5E075E090C@LSRIEXCH1.lsmaster.lifespan.org>

Yes, it is easy to do. Since silver chloride is insoluble in water, chloride added to a solution containing ionic silver will cause the white compound to precipitate out immediately.  It will settle out onto the bottom of the container overnight, or can be filtered out.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Friday, October 29, 2010 1:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Precipitating Silver

Has anyone heard of precipitating silver out of a solution by adding table salt? 
I read this somewhere as a means of disposing of silver easily. Any info would 
be appreciated.
Thanks,
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From wdesalvo.cac <@t> hotmail.com  Fri Oct 29 13:52:06 2010
From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO)
Date: Fri Oct 29 13:52:12 2010
Subject: [Histonet] PLP fixative
In-Reply-To: <44F1D6D7EB8CC84F92859EE5C4E6ECB4011B20F1B562@CVMMBX.vetmed.wsu.edu>
References: <44F1D6D7EB8CC84F92859EE5C4E6ECB4011B20F1B562@CVMMBX.vetmed.wsu.edu>
Message-ID: 


Follow the links for a receipe.
 
www.pathology.ufl.edu/~molecular/PLP%20Fixative.doc
 
http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/reagents.cfm
 
www.hopkinsmedicine.org/.../multimedia/text_documents/Recipes_For_Making_PLP_Fixative.doc


William DeSalvo, B.S., HTL(ASCP)




 
> From: ttruscot@vetmed.wsu.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Fri, 29 Oct 2010 10:55:45 -0700
> Subject: [Histonet] PLP fixative
> 
> I am seeking basic information on PLP fixative. What is it? Can I make it or buy it? How long does fixation take for lymph node? Thanks in advance, Tom Truscott
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From jhill <@t> vet.k-state.edu  Fri Oct 29 15:05:13 2010
From: jhill <@t> vet.k-state.edu (Jennifer Hill)
Date: Fri Oct 29 15:05:19 2010
Subject: [Histonet] IHC Staining Problem
Message-ID: <8AA2173DC209CA438077A832FF98BD7F02806442@VETMXHT.ads.vet.k-state.edu>

 I hope someone can help me with a problem I've been having with my CD79a hand stain.  This is a stain that was validated and working perfectly, when a few months ago I started having reduced, to no staining on my controls, with the occasional run staining a little stronger. I followed the discussion not too long ago with the person who was having similar problems, but the suggestions didn't seem to apply (I use Tween 20 in my PBS rinses, and we use APEX slides from Surgipath-not Fischer Plus).  I've tried everything I can think of and I hope someone may have some other ideas.  For reference this is for a veterinary diagnostic lab, not a human lab, and this is the only stain we've been having this issue with.

My protocol is as follows: After deparfinization/rehydration,
AR for 20 min in the steamer in Biogenex Citra Solution (I've gotten new Citra Solution, tried a pressure cooker)
5 min peroxide quench, followed by a 10 min protein block using Dako's Protein Block-Serum Free
60 min Primary incubation (Dako CD79a) at 37 C (decreased dilution from 1:100 to 1:65, new bottle of antibody)
30 min incubation at room temp of ImmPRESS anti-Mouse Reagent from Vector
Develop stain with DAB chromogen from Vector

Washes of PBS/Tween 20 follow between steps

Thank you,
Jennifer Hill
Research Assistant
Kansas State University
Veterinary Diagnostic Lab

From Laura.Miller <@t> leica-microsystems.com  Fri Oct 29 17:34:50 2010
From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com)
Date: Fri Oct 29 17:34:58 2010
Subject: [Histonet] Laura Miller is Out of  the Office.
Message-ID: 


I will be out of the office starting  10/29/2010 and will not return until
11/01/2010.

I am out of the office for the rest of the day.  I will be back on Monday!


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________

From pruegg <@t> ihctech.net  Fri Oct 29 17:38:02 2010
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Fri Oct 29 17:38:45 2010
Subject: [Histonet] RE: breast fixation times
In-Reply-To: 
References: <2820431BF953BB4DA3E9E1A5882265FD034A5780@MAIL1.pph.local><92AD9B20A6C38C4587A9FEBE3A30E1640448AF2A61@CHEXCMS10.one.ads.che.org><2820431BF953BB4DA3E9E1A5882265FD034A5783@MAIL1.pph.local><0908FC0A43B87A4FB60EDCCA06AABC2426C7E4@exchange.cmc-nh.org>
	
Message-ID: <0AD2F85778424E90B81B4CA2D469CDDC@prueggihctechlt>

Melissa,

Just because the clone you are using is not recommended in the guidelines
does not mean that you cannot use it.  You have to validate it the same as
using a clone they do recommend, but the bottom line is you can use what
ever you want in what ever way you want as long as you validate the protocol
on samples generated in your lab.  If you want to use a fixation or
processing different from what is recommended you have to compare it to
formalin fixation and "standard" (whatever that is) paraffin processing, you
would have to do a side by side comparison on the same tissue.  As long as
you follow the fixation and processing guidelines you can use the ab u want
to use without comparing it to abs they recommend, as far as I can tell.
You must validate that ab on at least 25 samples generated in your
institution just as you would have to validate an antibody they do
recommend. 

Regards,

Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla,
Melissa
Sent: Friday, October 29, 2010 3:51 AM
To: Feher, Stephen; Tench, Bill; Weems, Joyce;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: breast fixation times

One more question regarding ER/PR/Her2..who am I kidding, we will be
talking about them forever!!  With regards to the ER/PR publication: The
PR clone (Ventana 1E2) that we currently use is not listed as
'acceptable'. Is anyone else in this situation?  Are you switching to
another clone?? Have you come across anything speaking of this scenario?
What to do if you currently run a clone not mentioned?  It will be a
relatively easy validation for me, but I can't image I am alone here.
Thanks, Melissa

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher,
Stephen
Sent: Thursday, October 28, 2010 5:50 PM
To: Tench, Bill; Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: breast fixation times

Great discussion, comprehensive yet concise.  Thanks Bill and Joyce and
Melissa. 


Steve

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Wednesday, October 27, 2010 12:26 PM
To: Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: breast fixation times

My apologies for not including the updates accurate for ER and PR. 


Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

-----Original Message-----
From: Weems, Joyce [mailto:JWeems@sjha.org]
Sent: Wednesday, October 27, 2010 9:22 AM
To: Tench, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: breast fixation times

Thanks for this good explanation, Bill.

One can not follow the guidelines and document the variant in the
report, but not following them could hurt the patient if there is a
clinical trial they might participate in. Clinical trials follow the
protocol to the letter and if the FDA requirement is not met, the
patient can not participate. 

The times were extended for ER and PR to 72 hours, but NOT yet for Her2.
So...because the tissue is all the same, we must follow the 48 hour
limit. We just had a case this weekend. Had the clinical staff remove it
from the processor on Sun morning and embedded it Monday. We don't
ususally have this problem as we are a 6-day lab, but it was finished
too late on Fri. 

Cheers,j

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax 



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Wednesday, October 27, 2010 11:50
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] breast fixation times

There is no exception for core biopsies, as reasonable as that may seem.
I have had that discussion with the purveyors of the guidelines.  6-48
is the current standard.  there was a lot of discussion about exceeding
48 and using the FISH option.  My colleague responsible for this wrote:
It is in the CAP checklist, ANP 22998:

If the laboratory assesses Her2 by IHC or Her2 gene amplification by
in-situ hybridization (FISH, CISH, SISH), does the lab have a documented
procedure for ensuring appropriate length of fixation of specimens
tested? 

Specimens subject to Her2 testing should be fixed in 10% neutral
buffered formalin for at least 6 hours and no longer than 48 hours.
While fixation outside of these time limits is not an absolute exclusion
criterion for Her2 testing, labs should qualify any negative results for
specimens fixed less than 6 hours or longer than 48 hours. For cases
with negative results by IHC, consideration should be given to
performing confirmatory analysis by in-situ hybridization. 

 There is also a table in the original ASCO/CAP Guideline
Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol 131,
Jan 2007) that states that tissue fixed in formalin for greater than 48
hours is not an absolute exclusion criterion, but if known to be fixed
longer than 48 hours or unknown, the report should qualify any negative
result with this information (table 6). 

As for upcoming changes, i don't know other than these time limitations
are suppose to be more rigorously applied to ER and PR, along with the
newly instituted documentation of time between excision and time placed
in fixative.
 
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

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From historsd <@t> aol.com  Fri Oct 29 21:10:21 2010
From: historsd <@t> aol.com (historsd@aol.com)
Date: Fri Oct 29 21:10:51 2010
Subject: [Histonet] cryostat question
Message-ID: <8CD45EEBD9B9E32-EA4-3DF2@webmail-d038.sysops.aol.com>


Does anyone have 'hands on'  experience performing Mohs using a countertop cryostat?  Can the specimen head be manipulated to acccomodate a badly embedded block? Does the machine stay cold enough to perform Mohs? Any advice would be appreciated.  
My e-mail is historsd@aol.com. Thanks, Susan
From JMacDonald <@t> mtsac.edu  Sat Oct 30 00:44:58 2010
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Sat Oct 30 00:45:07 2010
Subject: [Histonet] IHC Staining Problem
In-Reply-To: <8AA2173DC209CA438077A832FF98BD7F02806442@VETMXHT.ads.vet.k-state.edu>
Message-ID: 

How far in advance are your controls being cut?  Are your patient tissues 
staining?




Jennifer Hill  
Sent by: histonet-bounces@lists.utsouthwestern.edu
10/29/2010 01:11 PM

To
histonet 
cc

Subject
[Histonet] IHC Staining Problem






 I hope someone can help me with a problem I've been having with my CD79a 
hand stain.  This is a stain that was validated and working perfectly, 
when a few months ago I started having reduced, to no staining on my 
controls, with the occasional run staining a little stronger. I followed 
the discussion not too long ago with the person who was having similar 
problems, but the suggestions didn't seem to apply (I use Tween 20 in my 
PBS rinses, and we use APEX slides from Surgipath-not Fischer Plus).  I've 
tried everything I can think of and I hope someone may have some other 
ideas.  For reference this is for a veterinary diagnostic lab, not a human 
lab, and this is the only stain we've been having this issue with.

My protocol is as follows: After deparfinization/rehydration,
AR for 20 min in the steamer in Biogenex Citra Solution (I've gotten new 
Citra Solution, tried a pressure cooker)
5 min peroxide quench, followed by a 10 min protein block using Dako's 
Protein Block-Serum Free
60 min Primary incubation (Dako CD79a) at 37 C (decreased dilution from 
1:100 to 1:65, new bottle of antibody)
30 min incubation at room temp of ImmPRESS anti-Mouse Reagent from Vector
Develop stain with DAB chromogen from Vector

Washes of PBS/Tween 20 follow between steps

Thank you,
Jennifer Hill
Research Assistant
Kansas State University
Veterinary Diagnostic Lab

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From miranda.felton <@t> gmail.com  Sat Oct 30 12:42:44 2010
From: miranda.felton <@t> gmail.com (Miranda D. Felton)
Date: Sat Oct 30 12:42:42 2010
Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 46
In-Reply-To: <4ccc4f42.4420ec0a.3409.1973SMTPIN_ADDED@mx.google.com>
References: <4ccc4f42.4420ec0a.3409.1973SMTPIN_ADDED@mx.google.com>
Message-ID: <8593425C-2AC9-4DE2-A3FD-6DBD73EDFEE1@gmail.com>

Does anyone know of a cytology listserv?

Thank you
Miranda

Sent from my iPod

On Oct 30, 2010, at 1:00 PM, histonet-request@lists.utsouthwestern.edu  
wrote:

> Send Histonet mailing list submissions to
>    histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
>    http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>   1. RE: lymph nodes peeling out of OCT (Monfils, Paul)
>   2. PLP fixative (Truscott, Tom)
>   3. Precipitating Silver (Sheila Haas)
>   4. RE: Precipitating Silver (Monfils, Paul)
>   5. RE: PLP fixative (WILLIAM DESALVO)
>   6. IHC Staining Problem (Jennifer Hill)
>   7. Laura Miller is Out of  the Office.
>      (Laura.Miller@leica-microsystems.com)
>   8. RE: RE: breast fixation times (Patsy Ruegg)
>   9. cryostat question (historsd@aol.com)
>  10. Re: IHC Staining Problem (Jennifer MacDonald)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 29 Oct 2010 13:13:44 -0400
> From: "Monfils, Paul" 
> Subject: RE: [Histonet] lymph nodes peeling out of OCT
> To: 
> Message-ID:
>    <4EBFF65383B74D49995298C4976D1D5E075E08F9@LSRIEXCH1.lsmaster.lifespan.org 
> >
>
> Content-Type: text/plain;    charset="us-ascii"
>
> I place all tissues in liquid OCT for at least 15 minutes before
> freezing.  This virtually eliminates the problem of tissue sections
> separating from the OCT after sectioning.
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 29 Oct 2010 10:55:45 -0700
> From: "Truscott, Tom" 
> Subject: [Histonet] PLP fixative
> To: "histonet@lists.utsouthwestern.edu"
>    
> Message-ID:
>     
> <44F1D6D7EB8CC84F92859EE5C4E6ECB4011B20F1B562@CVMMBX.vetmed.wsu.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> I am seeking basic information on PLP fixative. What is it? Can I  
> make it or buy it? How long does fixation take for lymph node?  
> Thanks in advance, Tom Truscott
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 29 Oct 2010 10:56:14 -0700 (PDT)
> From: Sheila Haas 
> Subject: [Histonet] Precipitating Silver
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <450265.9811.qm@web57802.mail.re3.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Has anyone heard of precipitating silver out of a solution by adding  
> table salt?
> I read this somewhere as a means of disposing of silver easily. Any  
> info would
> be appreciated.
> Thanks,
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 29 Oct 2010 14:02:26 -0400
> From: "Monfils, Paul" 
> Subject: RE: [Histonet] Precipitating Silver
> To: 
> Message-ID:
>    <4EBFF65383B74D49995298C4976D1D5E075E090C@LSRIEXCH1.lsmaster.lifespan.org 
> >
>
> Content-Type: text/plain;    charset="iso-8859-1"
>
> Yes, it is easy to do. Since silver chloride is insoluble in water,  
> chloride added to a solution containing ionic silver will cause the  
> white compound to precipitate out immediately.  It will settle out  
> onto the bottom of the container overnight, or can be filtered out.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- 
> bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
> Sent: Friday, October 29, 2010 1:56 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Precipitating Silver
>
> Has anyone heard of precipitating silver out of a solution by adding  
> table salt?
> I read this somewhere as a means of disposing of silver easily. Any  
> info would
> be appreciated.
> Thanks,
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 29 Oct 2010 12:52:06 -0600
> From: WILLIAM DESALVO 
> Subject: RE: [Histonet] PLP fixative
> To: , histonet
>    
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Follow the links for a receipe.
>
> www.pathology.ufl.edu/~molecular/PLP%20Fixative.doc
>
> http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/reagents.cfm
>
> www.hopkinsmedicine.org/.../multimedia/text_documents/Recipes_For_Making_PLP_Fixative.doc
>
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>
>
>> From: ttruscot@vetmed.wsu.edu
>> To: histonet@lists.utsouthwestern.edu
>> Date: Fri, 29 Oct 2010 10:55:45 -0700
>> Subject: [Histonet] PLP fixative
>>
>> I am seeking basic information on PLP fixative. What is it? Can I  
>> make it or buy it? How long does fixation take for lymph node?  
>> Thanks in advance, Tom Truscott
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 29 Oct 2010 20:05:13 +0000
> From: Jennifer Hill 
> Subject: [Histonet] IHC Staining Problem
> To: histonet 
> Message-ID:
>    <8AA2173DC209CA438077A832FF98BD7F02806442@VETMXHT.ads.vet.k-state.edu 
> >
> Content-Type: text/plain; charset="iso-8859-1"
>
> I hope someone can help me with a problem I've been having with my  
> CD79a hand stain.  This is a stain that was validated and working  
> perfectly, when a few months ago I started having reduced, to no  
> staining on my controls, with the occasional run staining a little  
> stronger. I followed the discussion not too long ago with the person  
> who was having similar problems, but the suggestions didn't seem to  
> apply (I use Tween 20 in my PBS rinses, and we use APEX slides from  
> Surgipath-not Fischer Plus).  I've tried everything I can think of  
> and I hope someone may have some other ideas.  For reference this is  
> for a veterinary diagnostic lab, not a human lab, and this is the  
> only stain we've been having this issue with.
>
> My protocol is as follows: After deparfinization/rehydration,
> AR for 20 min in the steamer in Biogenex Citra Solution (I've gotten  
> new Citra Solution, tried a pressure cooker)
> 5 min peroxide quench, followed by a 10 min protein block using  
> Dako's Protein Block-Serum Free
> 60 min Primary incubation (Dako CD79a) at 37 C (decreased dilution  
> from 1:100 to 1:65, new bottle of antibody)
> 30 min incubation at room temp of ImmPRESS anti-Mouse Reagent from  
> Vector
> Develop stain with DAB chromogen from Vector
>
> Washes of PBS/Tween 20 follow between steps
>
> Thank you,
> Jennifer Hill
> Research Assistant
> Kansas State University
> Veterinary Diagnostic Lab
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 29 Oct 2010 17:34:50 -0500
> From: Laura.Miller@leica-microsystems.com
> Subject: [Histonet] Laura Miller is Out of  the Office.
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>     >
>
> Content-Type: text/plain; charset=US-ASCII
>
>
> I will be out of the office starting  10/29/2010 and will not return  
> until
> 11/01/2010.
>
> I am out of the office for the rest of the day.  I will be back on  
> Monday!
>
>
> ______________________________________________________________________
> This email has been scanned by the MessageLabs Email Security System.
> For more information please visit http://www.messagelabs.com/email
> ______________________________________________________________________
>
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 29 Oct 2010 16:38:02 -0600
> From: "Patsy Ruegg" 
> Subject: RE: [Histonet] RE: breast fixation times
> To: "'Kuhnla, Melissa'" ,    "'Feher,  
> Stephen'"
>    ,    "'Tench, Bill'" ,  
> "'Weems,
>    Joyce'" ,    
> Message-ID: <0AD2F85778424E90B81B4CA2D469CDDC@prueggihctechlt>
> Content-Type: text/plain;    charset="us-ascii"
>
> Melissa,
>
> Just because the clone you are using is not recommended in the  
> guidelines
> does not mean that you cannot use it.  You have to validate it the  
> same as
> using a clone they do recommend, but the bottom line is you can use  
> what
> ever you want in what ever way you want as long as you validate the  
> protocol
> on samples generated in your lab.  If you want to use a fixation or
> processing different from what is recommended you have to compare it  
> to
> formalin fixation and "standard" (whatever that is) paraffin  
> processing, you
> would have to do a side by side comparison on the same tissue.  As  
> long as
> you follow the fixation and processing guidelines you can use the ab  
> u want
> to use without comparing it to abs they recommend, as far as I can  
> tell.
> You must validate that ab on at least 25 samples generated in your
> institution just as you would have to validate an antibody they do
> recommend.
>
> Regards,
>
> Patsy
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of  
> Kuhnla,
> Melissa
> Sent: Friday, October 29, 2010 3:51 AM
> To: Feher, Stephen; Tench, Bill; Weems, Joyce;
> histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: breast fixation times
>
> One more question regarding ER/PR/Her2..who am I kidding, we will be
> talking about them forever!!  With regards to the ER/PR publication:  
> The
> PR clone (Ventana 1E2) that we currently use is not listed as
> 'acceptable'. Is anyone else in this situation?  Are you switching to
> another clone?? Have you come across anything speaking of this  
> scenario?
> What to do if you currently run a clone not mentioned?  It will be a
> relatively easy validation for me, but I can't image I am alone here.
> Thanks, Melissa
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Feher,
> Stephen
> Sent: Thursday, October 28, 2010 5:50 PM
> To: Tench, Bill; Weems, Joyce; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: breast fixation times
>
> Great discussion, comprehensive yet concise.  Thanks Bill and Joyce  
> and
> Melissa.
>
>
> Steve
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
> Bill
> Sent: Wednesday, October 27, 2010 12:26 PM
> To: Weems, Joyce; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: breast fixation times
>
> My apologies for not including the updates accurate for ER and PR.
>
>
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> Bill.Tench@pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604
>
> -----Original Message-----
> From: Weems, Joyce [mailto:JWeems@sjha.org]
> Sent: Wednesday, October 27, 2010 9:22 AM
> To: Tench, Bill; histonet@lists.utsouthwestern.edu
> Subject: RE: breast fixation times
>
> Thanks for this good explanation, Bill.
>
> One can not follow the guidelines and document the variant in the
> report, but not following them could hurt the patient if there is a
> clinical trial they might participate in. Clinical trials follow the
> protocol to the letter and if the FDA requirement is not met, the
> patient can not participate.
>
> The times were extended for ER and PR to 72 hours, but NOT yet for  
> Her2.
> So...because the tissue is all the same, we must follow the 48 hour
> limit. We just had a case this weekend. Had the clinical staff  
> remove it
> from the processor on Sun morning and embedded it Monday. We don't
> ususally have this problem as we are a 6-day lab, but it was finished
> too late on Fri.
>
> Cheers,j
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 678-843-7376 - Phone
> 678-843-7831 - Fax
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tench,
> Bill
> Sent: Wednesday, October 27, 2010 11:50
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] breast fixation times
>
> There is no exception for core biopsies, as reasonable as that may  
> seem.
> I have had that discussion with the purveyors of the guidelines.  6-48
> is the current standard.  there was a lot of discussion about  
> exceeding
> 48 and using the FISH option.  My colleague responsible for this  
> wrote:
> It is in the CAP checklist, ANP 22998:
>
> If the laboratory assesses Her2 by IHC or Her2 gene amplification by
> in-situ hybridization (FISH, CISH, SISH), does the lab have a  
> documented
> procedure for ensuring appropriate length of fixation of specimens
> tested?
>
> Specimens subject to Her2 testing should be fixed in 10% neutral
> buffered formalin for at least 6 hours and no longer than 48 hours.
> While fixation outside of these time limits is not an absolute  
> exclusion
> criterion for Her2 testing, labs should qualify any negative results  
> for
> specimens fixed less than 6 hours or longer than 48 hours. For cases
> with negative results by IHC, consideration should be given to
> performing confirmatory analysis by in-situ hybridization.
>
> There is also a table in the original ASCO/CAP Guideline
> Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol  
> 131,
> Jan 2007) that states that tissue fixed in formalin for greater than  
> 48
> hours is not an absolute exclusion criterion, but if known to be fixed
> longer than 48 hours or unknown, the report should qualify any  
> negative
> result with this information (table 6).
>
> As for upcoming changes, i don't know other than these time  
> limitations
> are suppose to be more rigorously applied to ER and PR, along with the
> newly instituted documentation of time between excision and time  
> placed
> in fixative.
>
>
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> Bill.Tench@pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604
>
>
> [None] made the following annotations
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> prohibited. If you have received this e-mail in error, please notify  
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> ---------------------------------------------------------------------
>
>
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> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Fri, 29 Oct 2010 22:10:21 -0400
> From: historsd@aol.com
> Subject: [Histonet] cryostat question
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <8CD45EEBD9B9E32-EA4-3DF2@webmail-d038.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Does anyone have 'hands on'  experience performing Mohs using a  
> countertop cryostat?  Can the specimen head be manipulated to  
> acccomodate a badly embedded block? Does the machine stay cold  
> enough to perform Mohs? Any advice would be appreciated.
> My e-mail is historsd@aol.com. Thanks, Susan
>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 29 Oct 2010 22:44:58 -0700
> From: Jennifer MacDonald 
> Subject: Re: [Histonet] IHC Staining Problem
> To: Jennifer Hill 
> Cc: histonet ,
>    histonet-bounces@lists.utsouthwestern.edu
> Message-ID:
>     >
> Content-Type: text/plain; charset="US-ASCII"
>
> How far in advance are your controls being cut?  Are your patient  
> tissues
> staining?
>
>
>
>
> Jennifer Hill 
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 10/29/2010 01:11 PM
>
> To
> histonet 
> cc
>
> Subject
> [Histonet] IHC Staining Problem
>
>
>
>
>
>
> I hope someone can help me with a problem I've been having with my  
> CD79a
> hand stain.  This is a stain that was validated and working perfectly,
> when a few months ago I started having reduced, to no staining on my
> controls, with the occasional run staining a little stronger. I  
> followed
> the discussion not too long ago with the person who was having similar
> problems, but the suggestions didn't seem to apply (I use Tween 20  
> in my
> PBS rinses, and we use APEX slides from Surgipath-not Fischer  
> Plus).  I've
> tried everything I can think of and I hope someone may have some other
> ideas.  For reference this is for a veterinary diagnostic lab, not a  
> human
> lab, and this is the only stain we've been having this issue with.
>
> My protocol is as follows: After deparfinization/rehydration,
> AR for 20 min in the steamer in Biogenex Citra Solution (I've gotten  
> new
> Citra Solution, tried a pressure cooker)
> 5 min peroxide quench, followed by a 10 min protein block using Dako's
> Protein Block-Serum Free
> 60 min Primary incubation (Dako CD79a) at 37 C (decreased dilution  
> from
> 1:100 to 1:65, new bottle of antibody)
> 30 min incubation at room temp of ImmPRESS anti-Mouse Reagent from  
> Vector
> Develop stain with DAB chromogen from Vector
>
> Washes of PBS/Tween 20 follow between steps
>
> Thank you,
> Jennifer Hill
> Research Assistant
> Kansas State University
> Veterinary Diagnostic Lab
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 83, Issue 46
> ****************************************

From Gina.Rodriguez <@t> leica-microsystems.com  Sat Oct 30 16:01:54 2010
From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com)
Date: Sat Oct 30 16:02:03 2010
Subject: [Histonet] Gina Rodriguez is out of the office.
Message-ID: 


I will be out of the office starting  10/29/2010 and will not return until
11/08/2010.

I will respond to your message when I return.


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________

From Carol.Fields <@t> Northside.com  Sat Oct 30 16:23:12 2010
From: Carol.Fields <@t> Northside.com (Carol Fields)
Date: Sat Oct 30 16:23:26 2010
Subject: [Histonet] Medite Microtomes
Message-ID: <731941C266951A47BEF11E5EFAAED9C90655967D@nsmvexch01.northside.local>

Hi Netters,

Does anyone out there use the Medite Microtomes?  If so, have you had
any problems with them?  I purchased 3 of them less than a year ago and
I have already sent them back to Germany because of advancing (back and
forth) issues.  They are telling me (of course) no one else has had
these issues and they want to send me a third set of them (the 2nd set
are demos that are having some of the same issues).  I would appreciate
any feed back on their microtomes and company.
Thank you in advance....I'm afraid I have made a big mistake in buying
these.
Carole

Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fields@northside.com



CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege.

From jhill <@t> vet.k-state.edu  Sun Oct 31 14:29:59 2010
From: jhill <@t> vet.k-state.edu (Jennifer Hill)
Date: Sun Oct 31 14:30:04 2010
Subject: [Histonet] IHC Staining Problem
In-Reply-To: <544027.5024.qm@web120219.mail.ne1.yahoo.com>
References: <8AA2173DC209CA438077A832FF98BD7F02806442@VETMXHT.ads.vet.k-state.edu>,
	<544027.5024.qm@web120219.mail.ne1.yahoo.com>
Message-ID: <8AA2173DC209CA438077A832FF98BD7F02806542@VETMXHT.ads.vet.k-state.edu>

Thanks to everyone who has responded so far.  To answer the questions asked, our controls are either being cut the same day or the day or two before, so they are not setting for extended periods.  We have also used at least three or four different controls, all with the same results. (The controls stain fine for other tests: CD20, CD3, etc.)The pathologists are saying that the patient slides are not staining as well.  So it doesn't seem to be something so simple as a control issue.  As to the dehydration/deparafinization, that takes place on an automated stainer, and no other stains are having issues so I don't believe that is the issue either.


Jennifer Hill
Kansas State University
Veterinary Diagnostic Lab